LOCUS       DAOTAX010000001        87496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_197_length_87496_cov_30.413971, whole genome shotgun sequence.
ACCESSION   DAOTAX010000001 DAOTAX010000000
VERSION     DAOTAX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 87496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87496
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197_length_87496_cov_30.413971"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..233)
                     /locus_tag="PLM04_00005"
     tRNA            complement(159..233)
                     /locus_tag="PLM04_00005"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:His,seq:gtg)
     gene            345..1025
                     /locus_tag="PLM04_00010"
     CDS             345..1025
                     /locus_tag="PLM04_00010"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HON01501.1"
                     /translation="MNKQTIEQNNEVKEILQKLWLSRNGEVASQMRQYGMVYRKNLGV
                     AITRIREIASDYEPNEETAQQLWTLHGRETMLMAILLQPVNNFSWEMAQSWAEDCDNI
                     ETIEFACMHLFSKLPFVNELCCDWCASDKVQLQIMGFILSARNYQNFSDVDRNLIIEK
                     AFENSTTDNFHLYKAISLALCRFCRNGKSIADFILEKMSNFLLQPTAGQQYIMEEVKS
                     EMNFLELL"
     gene            1059..1532
                     /locus_tag="PLM04_00015"
     CDS             1059..1532
                     /locus_tag="PLM04_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HON01502.1"
                     /translation="MDENKEKEENSFEVVKKIFNDYLAAHQHRKTPERYAILEQIYTH
                     EGHFNAELLYNAMQKDYRVSLATIYNTLDLLLECKLIVKHQFGDQLAQYEKTFGTNIH
                     HHLICTQCGKIKEFSDKNIKAAIQSKHFQTFQMSHYSLYIYGICSKCLKKKKKTS"
     gene            1657..2925
                     /locus_tag="PLM04_00020"
     CDS             1657..2925
                     /locus_tag="PLM04_00020"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HON01503.1"
                     /translation="MKADVLLGLQWGDEGKGKVVDVLTPHYDIVARFQGGPNAGHTLE
                     FKGKKFVLRSVPSGIFQGDKINVIGNGMVIDPALFKEEVEALEAAGEPLHQRLKISKK
                     AHLILPTHRLLDLASEAAKGTGKIGTTGKGIGPAYTDKISRNGLRIGDILHRFDEKYR
                     EVVARHTEMLRQYNFEYDLAALEKTFFEGIECIKRFDLIDSEYFINHSLEEGKKILAE
                     GAQGTLLDIDFGSYPFVTSSNTICAGACTGLGLSPQKIGEVYGIFKAYCTRVGSGPFP
                     TELKDETGELIRKKGCEFGAVTGRPRRCGWIDLVALRYSIMLNGVTQLIMMKSDVLDD
                     FDTIKACVAYRIDGKETEEFPFEITENLEPVYVELPGWKTKISNLQSEDEFPEELNAY
                     LNFLEEELNVPIKIVSVGPDREQTVIRYQE"
     gene            3026..3703
                     /locus_tag="PLM04_00025"
     CDS             3026..3703
                     /locus_tag="PLM04_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HON01504.1"
                     /translation="MIYIIFIVFALISFLLQNRLQSKFKRYSKLPVPNGMTGKDVAEK
                     MLHDNGIYDVKVISTSGHLTDHYNPANKTINLSEQVYASSNVAAAAIAAHETGHAVQH
                     AYAYAPLTLRSKLVPVVSFASMWMQWVLLAGIIFLQSFPQLMLTGIILFALTTLFSFI
                     TLPVEINASQRALAWLNNAGITNYETHEPAKDALKTAAYTYVVAALGSLATLVYYILI
                     FMGGSRR"
     gene            3811..3884
                     /locus_tag="PLM04_00030"
     tRNA            3811..3884
                     /locus_tag="PLM04_00030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3845..3847,aa:Arg,seq:ccg)
     gene            4066..5472
                     /gene="rlmD"
                     /locus_tag="PLM04_00035"
     CDS             4066..5472
                     /gene="rlmD"
                     /locus_tag="PLM04_00035"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HON01505.1"
                     /translation="MRKKKQLPVLEKVTITDIGAEGKALTKINDMVVFVPLVVPGDVV
                     DLQVTQKKKNYMEARAVKFHHYSEQRCEPFCQHFGICGGCKWQMLPYAEQLKYKQKQV
                     EDHLKHIGKVQLPEISPILPSEKTVFYRNKLEFTFSNRRWRSFEEISSAQNIEDENAL
                     GFHIPGHFDKVLNIEKCWLQDDISNKIRNEIRRYALENELEFFDLRHQEGFLRTMMVR
                     TSTTGEVMLVVVFFREDEPKRKALLQHIADTFPEITSLLYVINSKANDTITDQEIISF
                     TGNDSIYEKMEDLTFKIGAKSFFQTNSAQACRLYEKARDFAALTGNELVYDLYTGTGT
                     IANFVARKAKKVIGIEYVPEAVEDAVNNSKLNNITNTFFYAGDLKEVMTANFIREQGN
                     PDVIITDPPRAGMHGDVVDAILTTNAARIVYISCNSATQARDLQLLDSKYKVTAVQPV
                     DMFPHTHHVENVVLLEHR"
     gene            5693..7123
                     /locus_tag="PLM04_00040"
     CDS             5693..7123
                     /locus_tag="PLM04_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HON01506.1"
                     /translation="MISFFISIGLLIAGYFIYGKIAERIFGIEPNRPTPALANPDGVD
                     YIPLPWWRVFLIQFLNIAGLGPIFGAIMGIMFGESAFLWIVFGTIFAGGVHDYLSGMM
                     SIRNNGTSLPELVGNELGNGIKQFMRVFSLALMILVGAVFVSGPAQLLANFTPHAINV
                     SGWIVIVFTYYILATLLPIDKIIAKIYPFFGFVLLFMAVGIFLALILNKAPIPEFTEG
                     FANLHPNKLPIFPIMFVSIACGAISGFHATQSPLMARCIQNEKLGRRIFYGSMVAEGI
                     VALIWAAAASSFFGSIQGLQEFLGTLPATANKPAVVVDRICRDWLGNIGGVITLLGVI
                     AAPLTSGDTALRSARLIVADFIHLEQKSWKKRLMISIPIFILTFIVLQINFDALWRYF
                     AWCNQTLAVFTLWSITVYLAKRHKGYWISLIPAVFMTCVSVSYICIAPEGFQLPQYLS
                     YLISAIACVVVIFFFYRFIYLNKEKY"
     gene            7338..8468
                     /locus_tag="PLM04_00045"
     CDS             7338..8468
                     /locus_tag="PLM04_00045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON01507.1"
                     /translation="MAITVREVTTKKQLKQFVWFGINLYKDNEYAAPPLFIDDYTNLT
                     KGKNPSLDFCEAAYFLAYNEKNEIVGRIAAIINPVANETWNQKNARFGWVDFIDDYEV
                     SKALFDAAVEWARAKGMTAMQGPLGFTDFDHEGTLIEGFDRLGTLAGIYNYPYYSQHI
                     EKYGFVKDADWVEFLITIPEKFPDKYFRIAELVKQKYHLTPRHFKSRKKLVNGYGNKI
                     FDLLNTCYQHLYGFTKLNEAQKQFYIKLYFSFFRLDTVSVVTDENDDVVAVGIAMPSL
                     TKALQKAKGKLFPLGWFYLLKSIYYKNDIVDLYLIAVHPRYQGKGVNSILFAELMVNF
                     AKNGYKFAESNPELETNTQVSSQWSDFEHINHKRRRAYIKQI"
     gene            8633..10219
                     /gene="nadB"
                     /locus_tag="PLM04_00050"
     CDS             8633..10219
                     /gene="nadB"
                     /locus_tag="PLM04_00050"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HON01508.1"
                     /translation="MTRKFDFLVIGGGIAGISYALKVAEYGKVALICKTTLEESNTSL
                     AQGGMAAVLYPPDNFEKHIQDTLIAGDYINNIEAVKKVVYEAPKQIEELIRWGVKFDK
                     AEDGKFDLHREGGHSEFRILHHKDNTGAEVQKRLNKKVLHHPNIEIFEYHFALEILTQ
                     HHLGQIVTHHTPNIECYGAYILDQKNNQIITFLSKVTMLATGGIGGAYLTSTNPPTAT
                     GDGIAMVHRARGVIKDMEFVQFHPTALYHPGDKPSFLISEAIRGYGAILRTKDGQKFM
                     HKYDERKELAPRDIVARAIDNEMKIRGDEYVYLDVTHKDPKETKKHFPTIYKRCRELG
                     INITKDYIPVAPTQHYLCGGIAVDLNAESTIHRLYAAGECSCTGLHGANRLASNSLIE
                     AIVYADAAAKHSVVRLNSLGFQENIPDWDDEGTTWPEEMILITQSAREVEQIMNNYVG
                     IVRSNLRLKRAMSRLEILFRETEDLYERSVVSRQICELRNIISVSYLIIKQATQRKES
                     RGLHYNIDYPGHAEDLKNKE"
     gene            10364..10753
                     /locus_tag="PLM04_00055"
     CDS             10364..10753
                     /locus_tag="PLM04_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON01509.1"
                     /translation="MKRTMLIVFWLALATMLLAGSYSPDDPRSSAQQVVVKSDVAVGQ
                     NWLRQDNSSGASDNQNQNIDNVSFSATSEGIYLSLGSGTYNVKLFALTGQLLWSGDLV
                     QGKFFIPVRKGIYFLRINGKSFKVVCK"
     gene            10825..11130
                     /locus_tag="PLM04_00060"
     CDS             10825..11130
                     /locus_tag="PLM04_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HON01510.1"
                     /translation="MVKHIVMFQLSDFSEGKTKKENATLIKSQLENLQHLIPELKKIE
                     VGIQMEETAAENYDLVLYTEFENVEDLQIYINHPEHKKVSAYIGKIKTGRVAVDYKV"
     gene            complement(11222..13681)
                     /gene="pheT"
                     /locus_tag="PLM04_00065"
     CDS             complement(11222..13681)
                     /gene="pheT"
                     /locus_tag="PLM04_00065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HON01511.1"
                     /translation="MNISYNWLKKYINIDLPVEKVSEILTSIGLEVGSTEEVQTVKGG
                     LEGLVIGEVLTCVPHENSDHLHVTTVNIGSGEPLQIVCGAPNVASGQKVVVATIGTKL
                     YAGDESFTIKRSKIRGVESFGMICAEDEIGIGTSHEGIIVLPDDAKPGMLAKDYYGIK
                     SDYVMEIDLTPNRVDAASHFGVARDLAAYFSLRDKSIKLTLPSVDNFQVQQHGLTIPV
                     TVENPEACPRYSSVTITGVKVGESPKWLKNYLTTIGLRPINNVVDATNFVLYELGQPL
                     HSFDADKIKGGKVIVRHMPEGTTFVTLDGVERKLSAEDLMICNAEEPMCIGGVFGGLD
                     SGVTESTQNVFLESAYFNPVYIRKTARRHGLSTDASFRFERGCDPNNTIYALKRCALL
                     IQEIAGGEIAGEIVDVYPKKILPFEVDITIAKINALIGKNFEKDTIQTILSALEIEIK
                     SSSEEGFVLKVPTYRVDVQRDVDVIEDILRIYGYNNVEIGEKLVSTLSYRPKPNNFRI
                     QNIISEQLTAQGFNEVMNNSLTKRNYYALSATLPLEKCVKIMNPLSSDLSVMRQTLLF
                     GGLENIVYNNNRKNFDIKFYEFGNCYFYLPENKNSSDPLSAYLEEFHLGLWLSGNKHP
                     QSWTSSEEKSSIFELKAYVENIFRRLGLKLQHFHFEEISNDLFSQALSFRNAAGKQLA
                     LYGIVNAALLKSFDIENEVFYADIDWNLLIEETKGKQVSFAEISKFPEVKRDLALLLD
                     KSVTFAEIEKIAYQTERKLLKKVQLFDVYEGKNIEAGKKSYAVSFLLQDETKTLTDSQ
                     IDAIMQKLLKNFTEKLGAKLR"
     gene            14019..14091
                     /locus_tag="PLM04_00070"
     tRNA            14019..14091
                     /locus_tag="PLM04_00070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14052..14054,aa:Gly,seq:gcc)
     gene            14127..14210
                     /locus_tag="PLM04_00075"
     tRNA            14127..14210
                     /locus_tag="PLM04_00075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14161..14163,aa:Leu,seq:cag)
     gene            14235..14318
                     /locus_tag="PLM04_00080"
     tRNA            14235..14318
                     /locus_tag="PLM04_00080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14269..14271,aa:Leu,seq:gag)
     gene            complement(14336..15253)
                     /locus_tag="PLM04_00085"
     CDS             complement(14336..15253)
                     /locus_tag="PLM04_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HON01512.1"
                     /translation="MKNEDRTSQAMHATWMGFWVNFTLTVFKLLAGFLGKSTAMIADG
                     VHSLSDFITDLIVIIFVRIAGKEKDQDHEYGHGKFETFATFLVSLALAAVGVYIFING
                     LNKVIQVTQGEIIEPPTIIALIAALVSIIVKEGLYRYTARIGKKINNQAIVANAWHHR
                     SDALSSIGTGLGIAGAIFFGEQARILDPIAGIIVSFFIIGVALKILLPSVNELLERSL
                     PEETKKEIFEIIESNPEILSYHNLKTRKVGNTFAIDIHIKLDKTTTFLRSHDIATEIE
                     NKLRNRFGKQTIINIHTEPFNEEKDQKRR"
     gene            15806..17020
                     /locus_tag="PLM04_00090"
     CDS             15806..17020
                     /locus_tag="PLM04_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HON01513.1"
                     /translation="MFRNQKITDDIYYVGVNDRQKAKFENLIPLYDGVSYNAYLIVDE
                     KTALIDTVDIHFGEILIQKILSQLGDKGLDYLIINHMEPDHSGSLRLLRKYFPNMTIV
                     GNERTLSMVKGFYEIDGSLLKVSDEESLSLGRHQLQFFLTPMVHWPETMMTFDHADKV
                     LFSGDAFGSFGALNGSILDENLDIDKYWDEMYRYYANVIGKYSSPVQKAIKKLEHLPY
                     EIICSTHGPVWKINLKKAVEIYDRLSSFQAEEGVVIVYGSMYGNTELMAETVAQGLAE
                     AGVKPVIIHNISTVDLSIILRDIYRYRGCIFGSPTYNNGLFPEMEDLIRKIESRGLKN
                     RIFGCFGSYTWSGAVVRNLTEFANRLQWPMSDVMVEEKQGLKKDNYESCLQLGHQIAE
                     MLQNPVQFNKGC"
     gene            17093..17320
                     /locus_tag="PLM04_00095"
     CDS             17093..17320
                     /locus_tag="PLM04_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HON01514.1"
                     /translation="MENRHSSGLGKINLVLIASGFAIIVIGFFLMTGSSTQVEFNPDI
                     FSFRRITVGPMVSFFGFLFIIFAILFKPKSK"
     gene            17323..18207
                     /locus_tag="PLM04_00100"
     CDS             17323..18207
                     /locus_tag="PLM04_00100"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HON01515.1"
                     /translation="MTFWQTIIIAIVEGLTEFLPVSSTGHMIITQSFLGIESTEFVKA
                     FTVIIQFGAILSVIVLYWNRFFRLQPLDEFNVPTYSEGKKFVKQRVIIRKFFYRYDFY
                     WKLLIGFLPAAVIGFLAGDFIDVLLGDVFVVALMLFIGGFVMLFVDKWFSASSQDQSI
                     GWQRALKIGFFQCIAMIPGVSRSMATIVGGMAVKLTRKNAAEFSFFLAVPTMAAASGY
                     KLFQLLSEQGGKDLLVQNWLLLLLGNVIAFIVALVAIRFFIDFISRYGFKAFGYYRIA
                     VGGVILLLLGMGYNLNMM"
     gene            18208..18906
                     /gene="truB"
                     /locus_tag="PLM04_00105"
     CDS             18208..18906
                     /gene="truB"
                     /locus_tag="PLM04_00105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HON01516.1"
                     /translation="MDVFKGEVFYVDKPLHLTSFNVVNRLRWKIKKVAGIKKIKVGHA
                     GTLDPEATGVMIICTGKATKQIESFQSQTKEYIAELKLGATTPSFDMELPVNAVYPTE
                     HITRELIDQVVSTFIGEIEQIPPIFSAVKIDGKRAYEYARNGQEIELKPKKLVIDEIE
                     ILNFVPPTLTLRIVCSKGTYIRALARDIGFALNSGSFLTSLRRIRIGNVRIDDCWDIE
                     NLLNHLDKELKSTE"
     gene            18968..20017
                     /gene="queA"
                     /locus_tag="PLM04_00110"
     CDS             18968..20017
                     /gene="queA"
                     /locus_tag="PLM04_00110"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HON01517.1"
                     /translation="MKLSQFKFKLPDELIAQYPPKHRDEARLMVVHKKTGEIEHRIFK
                     DVLDYFNENDLFIFNDTMVFPARLYGNKEKTGAQIEVFLLRELNAEMRLWDVLVEPAR
                     KIRIGNKLYFGEDDSIVAEVIDNTTSRGRTLRFLFDGTHEEFKKALYALGETPLPHYI
                     KRQVEAADAENFQTIFAKNEGAVAVPAAGLHFSRELLKRMEIKGIDMAFVTSHMSLGI
                     FRDIDVEDLTKHKMDSEQIIVPEETAKKVNKAQEEGRNICAVGVTVMRALETVAGTEG
                     KIKSFDGWTNKFIFPPYDFSVANAMITNFQFPYSTLLMLTAAFGGYDTIMHAYEVAIE
                     EKYRFGVYGDAMLIV"
     gene            20200..21399
                     /locus_tag="PLM04_00115"
     CDS             20200..21399
                     /locus_tag="PLM04_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HON01518.1"
                     /translation="MPHTSQRGQLMPESPIRKLVPLADKAKARGVKVYHLNIGQPDLK
                     TPQVALDTLKNIDLKILEYSPSDGFKSLRSKLAGYYDKFNIEATEEDIIITTGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAIAAGVVVRPVVASIEDGFALPPIEKFEELI
                     TPKTKGILICNPNNPTGYLYTRAEMNKIRDLVKKYDLYLFSDEVYREFCYTGGPYISA
                     FHLDGIDENVILVDSFSKRYSECGIRVGALVTKNKEVRKNVMKFCQARLSPPLIGQII
                     AEASIDTPAEYMLEMYNEYLERRKFLIDGLNRISGVYSPIPMGAFYTVARLPVDDADK
                     FCAWLLSDFDYEGSTVMLAPGSGFYTIEGMGKNEVRIAYVLEKEDLKKALVVLEKALQ
                     AYPGKTI"
     gene            21482..22723
                     /locus_tag="PLM04_00120"
     CDS             21482..22723
                     /locus_tag="PLM04_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON01519.1"
                     /translation="MNVEYFIAKRIHFQQGKKNVSRPAVRIATIGIALGLAVMLISVC
                     VVIGFKSQIENKIIGFGGHIQITNFDNNNTYEMNPIKMDAALIQKIRQISGVVHVQRF
                     ATKPGIIKTDNEFQGIILKGIDKDFDWNFFRSNLLEGNILNVGSDTLKNEILLSKKLS
                     DLLGLKLGDELFMYFIQDQVRARKFKIVGIYSTNFVDYDQLFVLTDIRHVQRLNNWDS
                     ASFGGLEVLIDNFDKIDERGEAVYEATANIFSKDGNAYYTQTIKQLNPQIFSWLDLLD
                     MNVWVILLLMLSVAGFNMISGLLILILERTNMIGILKSLGATNWSVRKIFLYHSFFLI
                     AKGMFWGNLIGLSICALQYFTGIIPLDPEAYYVASVPVAFPWGYIVLLNIGTFVASIL
                     MMIAPSYLITKISPAKIIRYE"
     gene            complement(22902..23966)
                     /gene="corA"
                     /locus_tag="PLM04_00125"
     CDS             complement(22902..23966)
                     /gene="corA"
                     /locus_tag="PLM04_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HON01520.1"
                     /translation="MAKYIKKRKKDIGVSPFELIFRGEKKMEKPRLYLMTYDENSVVE
                     TEIKSLRELNFNHSKDKFHWLNVEGLHEVKLMEKIAESFDIPFNIMSNIMDPMTRPQV
                     QDYEKGLFIVIKMLTYNEKLDKLDVENLSFILTKNTLILFQENQGDAFDPIRERLRKH
                     HHRIRTAGIDYLACALLDVVIDNYLYLIGQLGDKIEDVEELMTKELDNDIPSLINRYK
                     RELNYLRKYIMPAKEMILNLSKLESEFIRDDNHPYFKELQVNLNEVSELMDSYREILY
                     DQLSIYHNTLSSRLNDIIKILTIFSVLFIPLTFIVGVYGTNFDYFPEIHWQYGYFAMW
                     AVMLLIAGVMLWYFKKKKWL"
     gene            24232..24513
                     /locus_tag="PLM04_00130"
     CDS             24232..24513
                     /locus_tag="PLM04_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HON01521.1"
                     /translation="MNLDVSVIGYLAGFCTAVAQFPQAYKVYRTKDTRSISLGMYAIM
                     TFGVFLWLIYGILIQDWPMMLANGIGLIPCTFNLIITFQNVFRERKKMI"
     gene            24591..25013
                     /gene="aroQ"
                     /locus_tag="PLM04_00135"
     CDS             24591..25013
                     /gene="aroQ"
                     /locus_tag="PLM04_00135"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HON01522.1"
                     /translation="MKHIQIINGPNLNLLGEREPSIYGNKSFNGFFEELKKQYPDVKL
                     EYFQSNNEGEIIDKLQEIGFSADGIILNAGAYTHTSIAIADAIRSIQSPVIEVHISNV
                     FQRESFRHHSFLSEVCKGCIIGFGLNSYRLALEALLLI"
     gene            25062..26504
                     /gene="pyk"
                     /locus_tag="PLM04_00140"
     CDS             25062..26504
                     /gene="pyk"
                     /locus_tag="PLM04_00140"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HON01523.1"
                     /translation="MPKFTKIIATISDKRCDPDFIRDLFEAGMNVVRMNSAHLNREGF
                     LKIINSVRSVSNEIALLIDTKGPEVRTTIAENDKIILHAGDTIKIIGDPDKISTNECV
                     AVSYPFFVRDLHVGDDILFDDGEIELKVAGKDEQYLYCNVLNDGVLGSKKSVNVPGVR
                     INLPSLSARDKENILFAIENNLDFIAHSFVRNKQDILDIQQILDQHNSPIKIISKIEN
                     QEGVDNIEEILEYTYGVMIARGDLGIEVPQEKIPGIQRLLIRKCVMAKKPVIVATQML
                     HSMITNPRPTRAEVTDVANAIYYRTDALMLSGETAYGKYPVESVRTMSKIAEEAEKTK
                     LTENDIPIPTATVDITSFLANAAVEASSKFGIRAIITDSFTGSTARNLAAYRGKNPVY
                     AICYRERLTRELALSYGVFPKYQEEKRTNQEYLLLALRDLLDRKLLTEQDFFCYLSGS
                     FGEGHGTTFLEINQIEKVLKALNNYQLPSF"
     gene            26847..29273
                     /locus_tag="PLM04_00145"
     CDS             26847..29273
                     /locus_tag="PLM04_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON01524.1"
                     /translation="MKKVFFISFVFLFLSFEILASTVIKGRVIDARSGTPLEFVNVAL
                     YQLGSSVPSVGLTTDAKGNFEISSISSGKYVLKISMVGYQAYDKEVDIRSNILELGVI
                     RLEEESKVLNEVVVVAQGSQMKFDIDKKVFSVDQNIASSGGSATDVLQNIPSVEVDNE
                     GNVSLRNDANVEVWINGKPSGLTVENRAQVLQQMPAESIESIEIITNPSAKFNPEGTA
                     GIINLVLKKNRRGGYYGSLSAGVMYPDGAKLGSNGGINFNYSSSKIDGYVNMGYRSMN
                     MKGGGWNNRYNYLENDTTLLKQNNSSENNFDGVFMRAGLDWHLNDNNTFGISGFGMTH
                     NEDGNNNINYGLYDVDNFLLKNYSRYNLDNGNHPSFHLNVDYQHEFGRKDNNIMASIS
                     YSKHNRKSDDQYIQRDYLLDLTSSDITQTTDNRNHQWEFKIDYSNKITENSRLEAGWQ
                     SNLQERNNLTSAIDNLNNGVEIFSYSDNFDYKEQIHAAYLTYGNKFDQLSLQAGLRGE
                     YLEKKINNLETQSYFELFPSFYLSYSLPEQNELQLSYTRRINRPRGRMINPFRDYSDS
                     TSISYGNPQLLPEFASSLELNYLKTWNQHSLSASAYYRFTDNVIENVRYMNGQTMEST
                     YFNITKSKNAGLELVAKNNLFKILSLTSSLNLYYSQLDSASFTSPYNTTVTIPNQDDF
                     SWSGRLLANLMLSKNTSAQITGRYLSPRIISQGERAANYSLDLGFRQTFFDRKLNLNL
                     MVRDLLNSRKWKSTTWGTDFYQVSESYFHGRMVGMTLSYNFGNMKPKQTVKKRAESES
                     PLNMEMENIE"
     gene            complement(29306..30064)
                     /locus_tag="PLM04_00150"
     CDS             complement(29306..30064)
                     /locus_tag="PLM04_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HON01525.1"
                     /translation="MLSKNKIKFINSLNLKKFRDETNMFIAEGTKLINDLSVTFHCEL
                     LVVTPEWLENNTPIRTKELLEVNFEEFKKISNQKNPQGILAVFGKATDIALSLEEIGS
                     ELSLALDEIQDPGNMGTIIRIADWFGIKHIFASQTSADAFAPKVVQASMGALARVQVQ
                     EVDLENFLLPLKDKLPIYGTFLKGENIYTTPLSSNGIIVLGNEGNGISPAIEKLVTER
                     LFIPNYPPGNTTSESLNVSVAAALVCAEFRRRLK"
     gene            complement(30080..30529)
                     /gene="rpiB"
                     /locus_tag="PLM04_00155"
     CDS             complement(30080..30529)
                     /gene="rpiB"
                     /locus_tag="PLM04_00155"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HON01526.1"
                     /translation="MKPLNELKIAICSDHAGFELKNKLIEYLKSKNVGQIKDFGAYTA
                     ESSDYPDFAHPMASAVENGEFDFGISLCATGNGINMTVNKHKGIRAALCWMPEIASLA
                     RRHNNANVLSLPARFINENEAKQIVDVFFETDFEGGRHQRRIDKISC"
     gene            complement(30653..31804)
                     /locus_tag="PLM04_00160"
     CDS             complement(30653..31804)
                     /locus_tag="PLM04_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HON01527.1"
                     /translation="MAKILIIDDERSIRNTLKDILEFEKHQVSLAENGLKGLEAAQNE
                     MFDLIFSDIKMPEMDGIELLTQLKEKDIEAPIVMISGHGNIETAVECIKKGAFDFIEK
                     PIDLNRLLITVRNALEKKDLVVETKNLKKKTAKKYEMIGQSAALENVRQMIERVAPTD
                     ARVLITGENGTGKELVARLIHDKSNRANAPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIRQRIGKFEQADNGTIFLDEIGDMSLSAQTKVLRVLQENELSRVGSDKSIKVNVRVI
                     AATNKDLKKEIEKGNFREDLFHRLNVIPIHVPTLDERKEDIPLLVEHFTELICSEEGI
                     HPKKFEPDAIKALQQRSWTGNIRELRNVVERLIILGSPMITKDDVEKFA"
     gene            complement(31956..32975)
                     /gene="porQ"
                     /locus_tag="PLM04_00165"
     CDS             complement(31956..32975)
                     /gene="porQ"
                     /locus_tag="PLM04_00165"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HON01528.1"
                     /translation="MIKRIVLFLWFLITFVALVYPQAGKGVYQFLDLPVSSRIAAMGG
                     TNVSLKDNDINFSFCNPSLLTDKTNDFLSLSMANYLADIQYGSAVFGKNFKNKHYMAF
                     GMQYVDYGTFQETTEQNEIIGEFTAKDMALYIMYAYPITEKISVGGTFKPIYSAYERY
                     SSFGAAIDAGISYNDPTNFFSAGLVLRNIGSQIKGYYSDEEGQHYEPLPFNIQLGITK
                     KLAYAPLRFTGTLHNLQHWDLSYASSTQNETKKINFFDMALRHFIFNAEFIPSNNFYL
                     TLGYNFRRAKEMKVEDFKSQAGLSFGGGIKLYKFNVGFGMSTYQVGNSVYQFSVGTSL
                     SEFGL"
     gene            33269..33952
                     /locus_tag="PLM04_00170"
     CDS             33269..33952
                     /locus_tag="PLM04_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HON01529.1"
                     /translation="MEEKKSPKASLENKKSLFILIGLVMALSFIYIALEWTAREITIY
                     EGVQTDLKTEEELDIIQTTQDETPPPPPPPAPEVIEVLNVVENTVETQTINIETEDTK
                     NKAVDIIAAPVAAPIHEEDEQVIFQVVETMPQFPGGEQALFKFLNENIKYPVIAQENG
                     IQGRVICQFVVNTDGSIVDIQVVRGVDPSLDKEAVRVIQSMPKWIPGKQRGKPVRVRF
                     TLPINFKLQ"
     gene            34067..35290
                     /gene="hflX"
                     /locus_tag="PLM04_00175"
     CDS             34067..35290
                     /gene="hflX"
                     /locus_tag="PLM04_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HON01530.1"
                     /translation="METNNYQEKTILVGLVTPQQNREKAKEYIEELAFLAKTAGAVPE
                     KIFLQQLDHPNPETFVGPGKLEQIKNYLADHPVDMAVFDDELSARQIRNLESELKIKI
                     LDRTNLILDIFAKRAKTASAKTQVELAQYQYLLPRLTRMWTHLERQQGGIGTRGPGET
                     QIETDRRIILSKIAQLKQNLKEIDRQMSTQRKNRGKLVRVALVGYTNVGKSTLMNLLS
                     KSNIFAEDKLFATLDTTVRKVIIDNLPFLLTDTVGFIRKLPHHLIESFKSTLDEVREA
                     DLLIHVVDVSHPNFEEQIQVVNQTLKEITNEEKPMILVFNKIDAYNHKAKDPDDLTPK
                     KPENITLEEIQKTWMAKIHEDCVFISATKKQNIEELKKIMYQKIKAIHVQRYPYNDFL
                     YQDFEMEENETDEVE"
     gene            35320..37308
                     /locus_tag="PLM04_00180"
     CDS             35320..37308
                     /locus_tag="PLM04_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HON01531.1"
                     /translation="MKNYRLLTENEIHLLTSKGCTAENWEMVRVSEGFKPDFIADVHF
                     SGNIFLGNFNKIFVLAGGLQRHAGIRRCCLHNCTIEDDVYIDQVANYIANYHIGEGSY
                     IENVNLLVVEKDSTFGNGVKVPVMNEGGGREIPIFDFLSAPLAYVLTLYRHRPGLIQK
                     IQKLIDEYACKLKSDTGTIGKNVRIVNAGSIKNVRIGDCANIEGASCLINGSINSNAF
                     APVTIGAGVKCDDFIISSGVSVTDSTLISRCFIGQGCILGKHYSVLDSLFFSNCQGMH
                     GEATAIFAGPYTVSHHKSTLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGLAERGAKT
                     TSDSYLLWPAKIGAFTLVMGRHTKHSDTSDLPFSYLIENATDSFLVPAVNLRSVGTIR
                     DAQKWPKRDNRKDPKKLDPINFNLLSPYTIQKMMKGVEVLKNLQKISGETTEIYTYQN
                     CSIKNSSLLKGIDLYHIGIYKFLGNSLISRLKDKHFNTIEDIREILKPDTSVGSGEWI
                     DLSGLIAPKTEIEQLMKKIEDQPITLEDIQSEFEKMHEAYYNYEWTWAKDKLEIYWRK
                     PIAEVTREDLIQMVELWKNSVVTLDHLIYDDAKKEFSLNAKTGFGVDGDEQQKSLDFE
                     SVRGSFENNPFVLEVINHIKIKSELGDELLDRLKKVIN"
     gene            37338..38504
                     /locus_tag="PLM04_00185"
     CDS             37338..38504
                     /locus_tag="PLM04_00185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HON01532.1"
                     /translation="MNNFNFYSPTYFVFGKGRENEAGKYVKRFGGTKVLLHFGGNSAI
                     KSGLLDRVKKSLTQEGISFVELGGVLPNPRSGLVYKGIEICKNEGVDFILAVGGGSVI
                     DSAKAIAAGALYDGDFWDFYTGKKVEKALPVGTILTIAAAGSEGSSSSVITHENGLLK
                     RSAEGNALRPVFSILNPELTFTLPAYQTACGITDMMAHVMERYFTNTREVEITDRLCE
                     GILLTIIKEAPVAIAEPTNYGARANIMWAGMVAHNDICGVGREQDWATHQMEHELSAL
                     YDVAHGAGLAVMFPAWMKYVMNHDIMRFAQFAVRVWGCEMDFQHPEITAKEGIAKFEQ
                     FLISIGMPIRFAQLGAKAEDIPTLVKNMRLGNCTVGSFVKLTEKDVAEIYKLAV"
     gene            38613..39851
                     /locus_tag="PLM04_00190"
     CDS             38613..39851
                     /locus_tag="PLM04_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HON01533.1"
                     /translation="MEPKHLYFPSADIHLDILLPASKSISNRALIINALSGSRSEIRN
                     LSDCDDTKVMLRALKLDQSIIDVGAAGTAMRFLTAYLAMTEEEWTLTGSERMKQRPVR
                     LLVEALNDLGAEIEYLEKSGFPPLSIKGKKLTGGTIHFDGSVSSQYISALMMTAPYMN
                     EGLNIILEGEIISLPYILMTKNMMKEFGVEVNFLTNRIEIAPQFYKPVDFTVESDWSA
                     ASYWFEILSIAGEGEIFLKGLHQNSYQGDSKVAELFENLGVAVQYFSDGVLLTPNRKK
                     IDFFNYDFINQPDLAQTFAVTCCLKNIPFHLKGLKSLKIKETDRISALKTELAKFGFL
                     LTEPAEGELSWSGEKKDACHFEETGEISIKTYDDHRMAMAFAPAALLFPIAIEHPEVV
                     SKSYPTFWEDVEKIKKGKVN"
     gene            complement(40445..42574)
                     /gene="scpA"
                     /locus_tag="PLM04_00195"
     CDS             complement(40445..42574)
                     /gene="scpA"
                     /locus_tag="PLM04_00195"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HON01534.1"
                     /translation="MKPNFNNIDVSNITSSGAAMLQTEENWLTPEQIPVKPFYEKNDW
                     KGMEHLHYAAGIPPFLRGPYSSMYVTRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLPTHRGYDADHERVVGDVGKAGVSICSVEDMKILFDGIPLNKMSVSMTMNGAV
                     LPILAFYIVAGLEQGAKLEELSGTIQNDILKEFMVRNTYIYPPQFSMRIIADIFKYTS
                     QNMPKFNSISISGYHMQEAGATADLELAYTLADGLEYLRTGINAGLDIDAFAPRLSFF
                     WGIGMNHFMEIAKMRAARLLWAKIVKQFHPKNPKSMALRTHSQTSGWSLTEQDPFNNV
                     GRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICRQIDPW
                     AGSYYVESLTKELAEKAWDLIQEIENLGGMAKAIETGIPKMKIEEAAARTQARIDSGK
                     QIIVGVNRYTLEKEEPIEILEIDNTAVRMQQIERLKQLRASRNNEEVKKALDAITECA
                     RTGNGNLLELAVEAARVRATLGEISDACEKVAGRYKAIIRTISNVYSSETKNDANFQR
                     ASQLTEEFAKKEGRRPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEA
                     AKQAVENDVHVVGVSSLAAGHKTLIPQLIEELKRLGREDILVVAGGVIPPQDYDFLYK
                     AGVAAIFGPGTPVTKAACTIMEMLLAD"
     gene            complement(42749..43363)
                     /locus_tag="PLM04_00200"
     CDS             complement(42749..43363)
                     /locus_tag="PLM04_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HON01535.1"
                     /translation="MKRIIIPILIAIIFWFVMFSPWTKEYVNFWLTMTAAGITLTLMS
                     AFLGKDFKKQFSLSLKDVLIGLGSAVVLYVVFFLGDFFSKLLFDFAKDQVGQIYMMKE
                     GENPLLLSLLLILLIGPAEEIFWRGYVQRMLEPKFGSWMALIVTTLIYTLVHIWSFNF
                     MLIMSAMVCGAFWGLLYKYNKNLVTLIVSHAVWDVSVFILFPIV"
     gene            complement(43360..44262)
                     /locus_tag="PLM04_00205"
     CDS             complement(43360..44262)
                     /locus_tag="PLM04_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HON01536.1"
                     /translation="MKEKIKNGLLATRPWSFPASTMPALLTISYVFFLKNELGSEVNW
                     WFGVLALLGAAIFQASGNLIGDYFDYQHHVDRKETFGSSRMLVDQVFQPKTILHFGIV
                     LLAVGVVLGLFLCYRTGIDLLWIGAIGAMATYFYYKLKFAALGDLTIFIIYGPLIGLG
                     TAYVMTGQLMWFAVVLNIPVAFLVVNILHANNTRDIRDDGKANIKTQAMLLGIKGAKI
                     QYVVLSIGAYVGVVLMIIFKMIHPVALLMLLSLPLAVKNIKKMMTAEVEKPEKIFDLD
                     AQTAQLVLVFSLLFSISNFIAAWL"
     gene            complement(44339..46234)
                     /gene="mutA"
                     /locus_tag="PLM04_00210"
     CDS             complement(44339..46234)
                     /gene="mutA"
                     /locus_tag="PLM04_00210"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HON01537.1"
                     /translation="MAEKKLNLLADFPPVSTQEWLDKIIADLKGADFEKKMIWRTNEG
                     FNVRPFYREEDVDDLKITELLPGAFPYVRGTKSDNEWFVRQDIKVENAQEANAKALYI
                     LNRGVNSIGFQLKKADLSKEFVATLLKDIAADCVELNFTICISKAAELADILSEYFIA
                     QNYEVKKLQGSINFDPINRMLLAGKKLTKEEIAEKAKAVVQAISRLPFYRVIGVNATT
                     LSNAGSTITQELGYALAWGNEYLSMLIDAGVDKAVAAKKIKFNFGIGSNYFMEIAKFR
                     AARMLWALIVDAYQPPCPRPDCPNTVNGICKCAAKMRIHAETTIFNQTIYDAYVNMLR
                     TQTEAMSATIGGVDSLSVLGYDTAFKPSDEFSERIARNQQLLLKEEAHFSEVVDPAAG
                     SYYIETLTAEIANQAWKLFLNVENEGGFLAAVQNGKVQQTIKATANERLKAISNRKEI
                     LVGTNQYPNLLETAAEKIDKELQQCGCCSHEEGTEKLLPLRGAVEFENLRLAVEKTSK
                     RPKVFMLTVGNLAMRSARAQFSGNFFGCAGYEIIDNLGFSSVEEGVKAALDAKAEIVV
                     LCSSDEEYATLAPEAYKLLKDKTIFVVAGAPACMEDLKKEGIENFISIKSNVLETLKD
                     FNKKLGL"
     gene            46532..47758
                     /locus_tag="PLM04_00215"
     CDS             46532..47758
                     /locus_tag="PLM04_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HON01538.1"
                     /translation="MQKLIIFIFSVLFAFPLYAKRVMTGIEVLRADNFKLLEGKRVGL
                     ITNPTGVDGNLKSTIDILYEARNVKLVALYGPEHGVRGDVYAGDKVVNATDEKTGLPV
                     YSLYGTTRKPTSEMLKGIDVLVYDIQDVGCRSYTYISTMGLAMEAAAENDIEFIVLDR
                     PNPLGGEKIEGNLTEEPFISFVSQFKIPYIYGQTCGELALMLNEENALKKKCKLTVVK
                     MKNWKRSMKWEDTGLEWVIASPHIPHKHSAYYYPVTGILGELGYVSVGVGYTLPFQVV
                     AAPWIKAQEFADAMNRLHLPGFIFRPIFFTPFYGTLKGENCQGVQIHIVDFKKAHLSL
                     VQFYIMQELARLYPDKKVFDNANKERFSMFDKVSGTDQMRKLFQVNNSVSDIQHYWDK
                     DIQSYYQLSKKYYLYH"
     gene            47768..50602
                     /locus_tag="PLM04_00220"
     CDS             47768..50602
                     /locus_tag="PLM04_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HON01539.1"
                     /translation="MINSFLSEIKSFIPEKRIYTDELRLLAWGTDAGFYRLIPKAVVQ
                     SASEMEVSLLLRAATKHHLPVTFRAAGTSLSGQAISDSVLILAGSNWERYELAPDASF
                     IRLQPGIVGERVNQILKPFRKKFPPDPASIKSAMVGGIIVNNASGMSCGIRENSYRML
                     ISTRIVLPDGSVLDTGDEESKAVFAKTHPEFLRKIEDLRDEIRNDVTLKQLIEKKYRI
                     KNVTGLSINSFIDFDDPFDIITHLMVGSEGTLAFLSEAVMKTTTDYPYKASAMAYFDN
                     MHLACETVKNLKSFPVSAVEFFDRKAIRSVEDKFETISELKTLPDNSAALLIKIEASD
                     GNELNERIRQVEKVLNDAHTLLPVSFTDKESEYKKYWDMRSGIFPSVGSTRPVGTTCL
                     IEDIAFCIEDLAEATDDLHEIFIKNHYEDAVIYGHALDGNLHFIISQRFDTEEYIHQY
                     EKMMNEVIEMVVKKYHGSLKAEHGTGRNMAPFVKKEWGEKAFDIMLQVKQLFDPEGIL
                     NPGVIFNDNPRCHLENIKPMPAVHPLIDKCIECGFCEVNCVSAGFTMSSRQRIVVQRE
                     IKRLKTSDENSRRLKELEKSYRFMGEQSCAGDGLCATSCPVSINVGDYIHVLRNENMK
                     NKTMAQFLGRWTAKHFNWVASGLKSVLYIASAAQHILGNKAMGAITRGIHKWSGGVFP
                     LWTPAMPKRRNKIEVSTSTSTDSSLKVVYFPSCLNQMMGVSKNDPELTPLVEKTVSLL
                     HKAGYQVVFPKNMEKMCCGSIWESKGLLSVDEQKSSELEKALREASENGKIPILCDQS
                     PCLYHMRKSMKQLSLYEPVEFIHDFLLDKLDFYPIDEPITIHATCSTIKMGLQPKLVE
                     IAKLCSTQVLVPEEVGCCGFAGDKGFTMPELNAHALRKLRPQIEKAHVKVGYSNSRTC
                     EIGLNTNTGIPYQSIVYLVDKCTKNKINRS"
     gene            50623..52140
                     /locus_tag="PLM04_00225"
     CDS             50623..52140
                     /locus_tag="PLM04_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HON01540.1"
                     /translation="MRKFRLSVVLLFFAFSFLQAQPKREMRAVWIATVANIDWPSSNR
                     LLPTQQRIEIVNMLDRLTENNINAIVLQIRPMADAFYPSNLEPWSHWLTGRQGVRPSP
                     FYDPLQFIIEEAHKRCMEVHVWLNPYRVTTTGDSMMLSKEHLFYKQPELFVRYGGKYY
                     FNPGLDETRNFLNKVVEDIVEHYDVDGVHFDDYFYPYPVKGEDFPDDETFMKFPRGFA
                     PNQKADWRRNNVNMAIAEVQQTIKNLKPWVEFGVSPFGVWRNDNVDPRGSATRAGIQN
                     YDDLYADILKWLAEGTIDYVTPQLYWEIGKKVANYEVLLKWWIENSYGKNLYIGLIPS
                     SLGDKKNPAWTTGNEIIRQIKMNRNSSQVDGAVFFSAKTFLQNRQGLNDSLQSNYYRY
                     PALCPINRNILGEPSAQPQNLRVLRDGKDAFLLWDEVNEEGGCQTAYYVVYAFKGKEV
                     GDLNDPVNIIARTTENCIDLRILPHKFKGNYTFVVTAVNRFRQESIPTQGITRKL"
     gene            complement(52165..53589)
                     /gene="cls"
                     /locus_tag="PLM04_00230"
     CDS             complement(52165..53589)
                     /gene="cls"
                     /locus_tag="PLM04_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HON01541.1"
                     /translation="MSEIISIFFIALYVYTILTTISVLLLENRNPSKSLSWMLIILFL
                     PVIGLILYLIFGQDLRKQKIISRKSIHSLDHRDISSFDFSKINSDLLDAHQMNLIKLL
                     YRNSEAVGYTYNKIEVFSDGKEAFESIFQAVEQAREHIHLEFYIISDDSITNRLKDLL
                     IKKSHEGVRIRIIYDFIGSFNLSRKFLKTLTKAGIYVRPFFPLRFRISRSRINYRNHR
                     KLLVVDGKVAFTGGMNIADRYVYGNRLGYWRDTFLKIQGSAVHGLQHLFLSDWYFVEK
                     KLITDKKYYPVPAQFDNNLVQIVSSGPDTEWEAIMQGIAKAIMSAKKYIYIQSPYYMP
                     TELIEGCLQLAALSGIDVRIMIPTQSDSPIVIASTSSYLGKVLQAGVRVFYYRKGFLH
                     AKTVVMDDFVSIVGSCNIDYRSFYQNFEANAFVYEEKTALTLKELFLEDIHYCEEITL
                     EQWNNRKKTKKLMDSVSRLFSPLL"
     gene            complement(53590..54645)
                     /gene="ribD"
                     /locus_tag="PLM04_00235"
     CDS             complement(53590..54645)
                     /gene="ribD"
                     /locus_tag="PLM04_00235"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HON01542.1"
                     /translation="MDIEIKYMQRCLQLASLGDGYTAPNPLVGAVVVYNDTIIGEGFH
                     QHFGQAHAERNAIFSVKQPELLKDATLYVNLEPCSHYGNTPPCASLIVEKGIPRVVIG
                     TIDPNPKVSGKGIQILKDAGIETYVGVLEEKCRELNKRFFTFQEQKRPFIVLKWAQTI
                     DGFIDRIRNNVSEPPLIISNEITKQLTHKMRSENQSIMVSTNTVLLDNPSLTVRNWIG
                     KTPIRITLDRQGRIPENYHLLDGLVPTLVFTERQPAKLNNVEYIQIDFKKEGLRGILK
                     EIFDRNIHSVLVEGGAQLLNSFIAENLWDEANVEVAPIEIGNGIAAPVMTCQPASRKN
                     FDGHEWLHFYNPKKTIK"
     gene            54711..55574
                     /gene="prmC"
                     /locus_tag="PLM04_00240"
     CDS             54711..55574
                     /gene="prmC"
                     /locus_tag="PLM04_00240"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HON01543.1"
                     /translation="MDILYLCIMENILQYIHSELKGIYPESEIRFIGYLLLEKLTGFS
                     RTEILLNKNTQISCEAAKQLDGFLKKLKNHEPLQYVLGATEFCGLSFNVNPSVLIPRP
                     ETEELVEWICSENDRSKPLKMLDIGTGSGCIAISLKKNFPLAEVDAFDVSPEALKVAK
                     ENAKLNRVNVNFREIDIFNPPVFSKKWDLIVSNPPYVPENEKSEMQRNVTDYEPSLAL
                     FVPDNHPLKFYETITNFARLNLAKGGKLYFEIHYRSGKNMLDLLSQNGFREVQLKKDL
                     SGKDRMISGIF"
     gene            complement(55756..56394)
                     /locus_tag="PLM04_00245"
     CDS             complement(55756..56394)
                     /locus_tag="PLM04_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HON01544.1"
                     /translation="MNQRFVINIGRQLGSGGHLIGQGIARELGISFYDKELIQIASQE
                     SGLAKEFLEQLDEKKGQGFFRGMFGLRNSIMIDEYYFNDNYPSSEMLFKIQSDVIRSL
                     AEKQSCVFVGRCADYVLKDHPRAVNIFITADLPDRIARVCRNQKMTEEKAMDYIKKMD
                     KKRADYYNYYTDKQWGAAASYHLCINSSALGIEETISYIIEFVEKKLHFDKK"
     gene            complement(56785..57915)
                     /locus_tag="PLM04_00250"
     CDS             complement(56785..57915)
                     /locus_tag="PLM04_00250"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HON01545.1"
                     /translation="MKKIFLYLATVLMAGCSSSIKTIEISNTGSFDRNDEIIQINDKE
                     FLQLFEESDKWILKNNHNQEIGYQLVKNENGNLETFIFQAAVPANKKVAYHLKKGNPS
                     PVEPKTFARFVPERKDDFAWENDLAAYRMYGPALAGENPSNGVDLWLKRTKELIVDTF
                     YFNELQKGQSYHIDHGKGLDCYKVGHTLGAGGIAPFWNDSLWIGNYFNSYKVHYVGPL
                     QSKFSLFYDSIKVGDKFYKEQLTITVSAGTLLNKAEVTFNGYQQSLPLAAGIFLHENT
                     GKLSMNQEEGTAAYCEDAVSDAKVPSGRNYVGIFVPAPITKAMIKNNHALLISEYPPG
                     NTFVYFFGGGWNKWGFPTDNDWINAVNLFAQSYRSPLEVLIK"
     gene            complement(57990..58769)
                     /locus_tag="PLM04_00255"
     CDS             complement(57990..58769)
                     /locus_tag="PLM04_00255"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HON01546.1"
                     /translation="MNQFDLKGKIALITGASYGIGYAIAKAFHAAGAVIVFNDINQEK
                     VDMGLASYKADGIDAHGYVFDVTDEEAVTEAIAKITKEVGIIDILVNNAGIIKRIPAL
                     EMSAKDFKQVVDIDLNGPFIVSKAVAPGMIAKGGGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNLACEWGAFNIQVNGIGPGYISTPQTAPLREEGHPFNTFIVTRTPAGRW
                     GTPEDLQGPAVFLASSASDFVNGHILYVDGGILAYLGKQPS"
     gene            complement(58849..59691)
                     /gene="kduI"
                     /locus_tag="PLM04_00260"
     CDS             complement(58849..59691)
                     /gene="kduI"
                     /locus_tag="PLM04_00260"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HON01547.1"
                     /translation="MKTNYEIRYASHPQDAKNYDTSRLRKEFLVERIFTINEVNMVYS
                     LFDRMLVGGAMPVAEVLTLESIDPLKSDFFLERREIGIFNVGGKGCINVDGTNYELDY
                     KEALYIGRGNKKVTFESVDGSNPAKFYFNSATAHKACPTRKITKENAVILEMGSLEGS
                     NHRFINRMMVSQVLETCQLQMGMTELEPGSVWNTMPAHTHSRRMEAYFYFELPDDQAV
                     CHFMGEPEETRHIWMKNDQAVISPQWSIHSAAATSNYTFIWGMAGENLDYDDQDFYKK
                     TDLK"
     gene            complement(59976..60605)
                     /locus_tag="PLM04_00265"
     CDS             complement(59976..60605)
                     /locus_tag="PLM04_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HON01548.1"
                     /translation="MRKISIFLCIFLFSANLFAQPSSEKPEIYFGTTQGISASMINFS
                     PSVNQGMLLGYNGGLIFRHITEKNVGLQVELNFFQRGWKETDSIYSRRLNYIELPFMT
                     HIYFGNKSRFFFNIGPKISYLLTENVLKNETVSSEKVQHVKSVENPFDYGICGGLGFM
                     FRIRKNVLQLDARANYSLSDIFSNNKTDFFDSSNNINFSVNLGWLWQVK"
     gene            complement(60610..62547)
                     /locus_tag="PLM04_00270"
     CDS             complement(60610..62547)
                     /locus_tag="PLM04_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HON01549.1"
                     /translation="MKKNIFCLLTVIILSLTGFSMEQTNSYPIKKVNGIEYYVYTVQS
                     GDGLYAISRKFGIAPEEISKVNPEIQKGLKSGQKILIPIQKNVQENEDASQRLKNELS
                     LLQEKSFEENQKNLHQQTDNSLPSTTLFLQHKVEKNQTLFGISKKYNVSEEEIIQYNP
                     QSKNILREGDVLFIPYKTQTTKNETEIKGDLVEVITPSGKKELNEKQLPSKDKPQYIK
                     HLVKPNETLFSISRTYNVTIDDILKLNPEAFPVLKIGTELTIPTSILKKDSIPSTTLN
                     NNEMDSALQDIENPEITTALFPENNIKIAFLLPFNLDTPSQQNANGRFRDFYAGSLLA
                     IKKAKENNYSLEIFTFDTEKSDEKLKEILAKPEMKSVDLIVGPAYSNQISLISDFAKE
                     NKINVLIPFSSKVYEIGINPYLFQFNPNDTQIEIDFLVDKIIQKTYRNANIVFVNLPG
                     ISILDEGNLFMERLKNRLLKERHKFNEFNFSSSNKNQSIPEVLKRNQKNLLFFNTDNS
                     SLITPFLKEFYVYIDEFDMTLYQEYGWENQHIERFDRIYVSPFKPEIDASALEEYQKT
                     FHQFFEWEVESERPRYDLLGYDLLNCFIPILKNYENNLTKKLKEITFDEGIQSDFHFK
                     KQSEFSGYINDKLYMGERKAK"
     gene            62744..63991
                     /locus_tag="PLM04_00275"
     CDS             62744..63991
                     /locus_tag="PLM04_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HON01550.1"
                     /translation="MKKLTISFCFYLIVLSVSAQLVATDKYAVYQNFTGVDYIFVFNG
                     ILPGTEIRFTGSGDNVKWYRFSDTTTPYSTLHYIFPEDASGYILDVDGHQTSVWVIDY
                     QNYLPVFHTLASDESSDNRCNELTLLLDANVPELYYYTLSGIRRKINRNFTLSYQTLE
                     WDNEWKTVETTKNVTLPVTEITVPAPLCNTTFSLQGDQFTSEPSLGIQPIGIISSMYA
                     AVSLECHIKTSTTARTEKNEDERPTSDALSGSAPLEMLFNAEVNNAGTIFYKWDIYKD
                     NQLLVSRSDKEHRYTFTEAGTYNVKLTVNNDFCSSSDSVSISVSTSDLQVPNVFTPNG
                     DGINDEFRVAYKSLISFDARIYNRWGRMVYHWNDPQKGWDGTIGGKKAPVGAYFYVIK
                     AIGADGIKYSKKGDINLLRGREN"
     gene            complement(64051..64833)
                     /gene="ubiE"
                     /locus_tag="PLM04_00280"
     CDS             complement(64051..64833)
                     /gene="ubiE"
                     /locus_tag="PLM04_00280"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HON01551.1"
                     /translation="MNKEKTTSQYTFQEVKPYNEKEGKTEQLVRMFDTIAAQYDKFND
                     LMSWGFAKLWRKRALSTLKRFRPKHILDVAAGTADMCIKAFRLLNPDKVTGIDISPKM
                     LEIGKEKIEHAQLSDKIELKVMDCAQLNFDDNSFDAVTIAFGVRNFEKLEESLHQIFR
                     VLKPGGHLLILELNEPQKGWLMKGYQLYIKLFVHQTAKYLSSDQNAYRYLTSSMHAFP
                     KGKEMIEILQNNGFELVECKKFTFGVCSMYLMEKPTHSKIDG"
     regulatory      65014..65233
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            65399..66127
                     /locus_tag="PLM04_00285"
     CDS             65399..66127
                     /locus_tag="PLM04_00285"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HON01552.1"
                     /translation="MKKKSLIVLSTLLLIFFTSCENDSDFKLKTTVDFEDVELGENGY
                     WNGSDFSKKFVSNGFIFLNDFTSSPWGDFWSGFACSSLTDTITAGYDNQYSVAAGSGA
                     WGSNKFAVAFDDSATVNCPKNELGTYEAKSIMLCNSTYAYLDMRNGSAFSKKFADGDW
                     FKVTIKGFLNNNATGAVDYYLADFRNGKHYLCHQWVKVDLSSLGKVDQLIFLFDSSDK
                     GTFGVNTPKYVCIDNLEFEQDAEN"
     gene            66482..67066
                     /locus_tag="PLM04_00290"
     CDS             66482..67066
                     /locus_tag="PLM04_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HON01553.1"
                     /translation="MEYTTLQGKLIMPEYGRNIQQMIAYAMTIEDREERTRCVKTIIN
                     IMGNLFPYLRDVNDFKHKLWDHVAIMSDFKLDIDFPYEVLRPENLYTRPEKIPYKNSD
                     IRYRHYGRTLEEMIEKVADYPDGEEKNELIRLIANQMKKCFLTWNKEVVDDRKIFDDL
                     RELSKGKLDISEDFLKLVESKEVLHNKKNKKKNK"
     gene            67117..68427
                     /gene="murA"
                     /locus_tag="PLM04_00295"
     CDS             67117..68427
                     /gene="murA"
                     /locus_tag="PLM04_00295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HON01554.1"
                     /translation="MSSFQIEGGHRLSGSITPQGAKNEALQVICATLLTSESVIIRNI
                     PDILDVSHLLSLLKDLGVKISKIEKGTYSFKADSLNMDYLQSEDFYDKSSSLRGSVMI
                     LGPLLARFGKALVPKPGGDKIGRRRLDTHFSGIQKLGADFRYNETKKQYEVSAKKLKG
                     TYMLLDEASVTGTANILMASVLAEGKTTIYNAACEPYIQQLCRMLIAMGAKISGVGSN
                     LLTIEGVKQLNGCEHTILPDMIEVGSFIGMAAMTGSEITIKNVAYDELGIIPDSFRRM
                     GIQVLQKNDDIYIPAHESYTIETFIDGSIMTIADAPWPGLTPDLLSVFLVIAAKANGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGNQLRLRASVMSSPDIRAGI
                     ALLIAALAADGTSIIHNIDQIDRGYEHIDTRLNALGANIERIQF"
     gene            68463..68975
                     /locus_tag="PLM04_00300"
     CDS             68463..68975
                     /locus_tag="PLM04_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HON01555.1"
                     /translation="MLNDKITEMLNNQINKEFFSWYLYLDISNYYANKNLDGFANWFS
                     IQAQEEYAHAQLFLKYILNNGEKVKLLPIAAPESNFVDFKAPLSAAFEHELFITASIN
                     EIYAEAYAQKDFKTMQFLDWFVKEQGEEEKNVDDLCKRFDLFGTDPRGLYLLDNELAS
                     RVYTAPSLVI"
     gene            69761..71020
                     /locus_tag="PLM04_00305"
     CDS             69761..71020
                     /locus_tag="PLM04_00305"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HON01556.1"
                     /translation="MKNILSTVVFIFLFVNSSLTAANPYRTKILKPNIKTLQIRISDT
                     DFGFPILKLNGTETLTVEFDEMSHNVRSFGYTVLHCNADCTVSNLNSNEYVDGFTRGN
                     IDDYAFSQNTTFQYIHYRFTIPNQDMRFKISGNYVVLIYEDNRVDDPVATACFSIVEP
                     KVEIDARVRANTDIELSGRYQQLDIEVNLANYVVQDPASEVKLVVRQNNRFDNEVANI
                     TPTFFNAGKLSFVNNKALIFEGGNEYRHFDISSFYAAGEGVDRIIFNRKYYEAFLKED
                     LMQKSRTYISQPDANGRFIINFQEAFQDVQTEADYVQVHFSLLSEQPFFDGQVFIGGD
                     YNYNLLDDTARMQYDFDAQRYTKTLLLKQGGYSYQYWFLPKGKNSASTGKIEGSFWQT
                     KNEYTIYFYHRAWGERYDRLIGYSVID"
     gene            71323..72819
                     /locus_tag="PLM04_00310"
     CDS             71323..72819
                     /locus_tag="PLM04_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON01557.1"
                     /translation="MNQEDTFPTTDSLKDFQQNFTKEPTIENLKNALNNEIFNIISDI
                     ADEAHQECYVIGGFVRDLLLRRPSKDIDILVVGNGIELAEKVVNQLKQLNKPCKFAIF
                     KNFGTAQIKLKEMDVEFVGARKESYRSDSRKPIVEDGTLQDDQNRRDFTINTLALCLN
                     KSRFGELVDPFNGLRDLQNKVIRTPLNPDITFSDDPLRMMRCIRFATQLNFFIETNTF
                     EAIGRNKKRIEIISKERITDELNKIILSPKPSIGFILLDKSGLLDFILPELSALKGVE
                     TKDGIGHKDIFYHTLMVLDNLSQTSDSLWLRWAALLHDIGKPRTKRFDKRQGWTFYNH
                     NIVGEKMTADIFRRMKLPTNEKMKYVQKMVSLHMRPIVLSEEEVTDSAVRRLLFDAGD
                     DIDDLMLLCEADITSKNPEKVKRYRENFQLVRKKLKEIEEKDRIRNFQPPIDGKEIME
                     TFHLPPCALVGEIKMKIKDAILDGIIPNEYEAARSYMMQIAAEMGLTN"
     gene            72833..74107
                     /locus_tag="PLM04_00315"
     CDS             72833..74107
                     /locus_tag="PLM04_00315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HON01558.1"
                     /translation="MKKIFYFIPLLLLSCATMAKNNGNPLKFKHSERSFAAFDTERLA
                     RLDTVFQDLVRKGEIPHAVTFVAHRGNIVHFKAFGWRDKEKNIPCQRDDIFRMASQTK
                     AITATGVLMLMEEGKILLDDPVKKYIPEFAHPQVLVSFNEKDSTYTTRPANKDITIRH
                     LLTHTSGISYGTDPNNPMMKIFAKKGIPPLNSLDPVTLKEVVKKLASCPLEHDPGERF
                     TYGMSVDVLGYLIEVVSKMPVDQYFKEKIFMPLGMNDTYFYVPKEKQNRMVTLYQKVQ
                     GKTLQPSSSVLYQTFPYSGAQTLFSTGAGLSGTIEDYAKFCQMILNKGEFNGKRILGR
                     KTVELMCRNQVGDLRGEIGFGLAFDDFRPQYLHLTMASEGSLRWGGMFGTDYLIDPKE
                     DLILLFYINWQTDNSVPDVKVLFHNLVYQALK"
     gene            74139..76253
                     /locus_tag="PLM04_00320"
     CDS             74139..76253
                     /locus_tag="PLM04_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HON01559.1"
                     /translation="MKAVFILSFSIVFMAACHSFSKIAYPHTQKENVTDHYFGTDVAD
                     PYRWLENDTSAATAAWVKAQNEVTFKYLEKIPFRNALKNRLTQLNDYPKLGAPFKKHG
                     KYYFFKNNGLQPQSILYQQSTLYSEPEILLDPNTFSSDGTVALSSVSFSNVGNYMAYS
                     VAKSGSDWNEIFVLNLDNRQTLSDHIQWTKFWNVAWKDNGFYYIAYDAPQKGKELSAK
                     NEFPKVYYHQIGQLQSKDLLIYQDKNHPLREFGPFVTEDEKYLFLIITETTTGNALLM
                     KDLTDPKAKFVSLVDGFENDYSVIDHINGKIYILTNWKAPNKRIMEVDPAHPSRENWR
                     EIIPEQKTVLESVEIIDGKFFCLYLKDAAHRLYAYDLSGKLLYEVKLPALGSVVSLTG
                     NKDDSEAFFAFSSFTYPPTIYRLDAGKNVSEIFQKAQVKFKPEDFVTEQVFYTAKDGT
                     KIPMFLSYRKGIKKDGKNPLMLYGYGGFNISMTPSFSISRIPFLENGGIYAVANIRGG
                     GEYGESWHMAGTKMQKKNVFDDFIAAAEFLIAEGYTSSKKLAINGGSNGGLLVGACLT
                     QRPDLFAVAVPEVGVLDMLRYHLFTIGWSWASDYGTSEESREMFEYLKSYSPLHNVVK
                     GTKYPAVLIMTGDHDDRVVPAHSFKFAATLQEANAGNKPVLIRVDNNAGHGAGKPLGK
                     LIDAQTDMWSFVMYNLGMKPKW"
     gene            complement(76308..77999)
                     /locus_tag="PLM04_00325"
     CDS             complement(76308..77999)
                     /locus_tag="PLM04_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HON01560.1"
                     /translation="MNDSYPYRNFGFRNNLDKVKRSRQIISVFVKFGLDYLFEASRVN
                     FLSKLRKRRKVYHKLSTAERLRLAFEELGPTFIKFGQILSTRPDFLPAEYIRELEKLQ
                     DQVLPMETPLVRRCIEKELKHTLEELFLDFDQSPVASASLSQVHKAVLQNGDTVALKI
                     QRPDIHKTIETDLSILESFAEILEKRLRNSSLYHPTAIVAELKKTIRKELDFMNEAHN
                     FEKFRKNFKNVDYIQVPKVYWQFTTEKLLTMEFIEGVKITEITQEKYEHRYEPKKVAD
                     RAAKALLKQILIDGFFHADPHPGNLFIQPPATIVMLDVGMTGHLDEESIITIARILRA
                     VNEKDSNHVIKGLQELGIVVKETDKRQLRHDLTELIEMYADRPFRTISLNRMNQQLLE
                     IMARHDMALPSNLVMMLRAMSIAESIGQQLEPDFDMFAIVEPFVKRIYLKLYSPKSFR
                     KRSMNVMDESIELIELFPQNISDLLGKLKEGQFEIKLEHQNLENLQDEMKSSSNRISA
                     SLIIAALIVGSSLILQQRIGPLISDISILGVIGYLLAGIMGLGLLLTIFKTRRKK"
     gene            complement(78004..78321)
                     /locus_tag="PLM04_00330"
     CDS             complement(78004..78321)
                     /locus_tag="PLM04_00330"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HON01561.1"
                     /translation="MADVIKNAFLAGLGLVSLTREKAEEFAKDLIKRGELTETEKPKF
                     IKDLLEQSEKTKTEMEEKVEKAVDAILKKMNIPSRKEFEELKNKVEELTQLLNKKQEE
                     SEN"
     gene            complement(79063..79629)
                     /locus_tag="PLM04_00335"
     CDS             complement(79063..79629)
                     /locus_tag="PLM04_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HON01562.1"
                     /translation="MACIWKKRLKRNFSKLQVGDKVIGYFSGKSKSFVSIGVIEKSLS
                     QDPNKKEIAIRKICDLQSPMSLGRVRQIKPFDNKLSNNGKLNTTVIPLTLDEYNLLMS
                     IIHGAFLDVYDDVDSDLNSVDMPIKEALKLRPAKQISNSTTWIRDSRISKAALKHANY
                     ICEFATKSDNHESFIANYTKKIMLKHTI"
     gene            complement(79871..80605)
                     /locus_tag="PLM04_00340"
     CDS             complement(79871..80605)
                     /locus_tag="PLM04_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01563.1"
                     /translation="MSKSYLIVFHGQIVNDKIVDIGDDPCFDCPPSWGICRPSTRKSV
                     KVGDTLIFIAKVDNDYFLKGWFKVGNKLDYPFALQQFPSRQNVIISKTISSHPIKWRY
                     KNLQKSYYKTHGQTTPNFLIQLDTGSGTFYQSQTDDHETDNWKCRRIFHCTSKQFEKC
                     IAANNCLKSSELLTANQYKNYVVADKNHWADLDNLRITFDEIKKATNFPKKIRTPKGQ
                     HNVMRFDDYKEKLFAFIEKRKNDAKK"
     gene            complement(80793..81731)
                     /locus_tag="PLM04_00345"
     CDS             complement(80793..81731)
                     /locus_tag="PLM04_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON01564.1"
                     /translation="MKQILITGGAGFVGSHLCERLLNEGNKVICLDNFLTGNKQNINH
                     LIENPNFKIVEHDICEPYFTEIDEIYNLACPASPIHYQYDAIKTVKTSVLGTINVLGL
                     AKRTNAKILQASTSEIYGDPIAHPQNESYWGNVNPIGIRSCYDEGKRCAETLFMDYHR
                     QNNVRIKIARIFNTYGTHMQANDGRVVSNFIVEALQNKDITIYGNGQQTRSFQYVDDL
                     IDGLIRLMNTRDNFVSPVNIGNPTEFTILELAEKIIDLTNSKSKIKFFSAVQDDPKQR
                     CPDISLAKEVLNWQPKIDLESGLKKTIEYFENILTD"
     gene            complement(81728..82687)
                     /locus_tag="PLM04_00350"
     CDS             complement(81728..82687)
                     /locus_tag="PLM04_00350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON01565.1"
                     /translation="MSYLSNVPYGKILYYWYEKLSHNPYRKKFLWTKIIEIIAFKRHS
                     SKQNNKIYQYVDFLQPIANDISENPIVSIVVPTFIRNQKDINDIENLLLSISKQTVKP
                     DNIIIVDDFSPISYSFPEHIQVYRQTKNSGPAKARNWAKKNAVENKSDIIAFTDTDCI
                     LSENWVETIIKSFQQSKDFQILSGNTISYDKNWFGIYHNINGTLNGRKFKDSERLLYG
                     TTANLSTTRQVAENIDFSEEFSLAAGEDIEFCFRANHDCFAIKHIPQMIVFHNYGYTN
                     GLWKNLKSFTRQFMRYGQGEVTLLQEIPEYYVYFDKTEEITAV"
     gene            complement(82691..83512)
                     /locus_tag="PLM04_00355"
     CDS             complement(82691..83512)
                     /locus_tag="PLM04_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HON01566.1"
                     /translation="MSFEKYKTILHQIGDKTKTIRLNGRGESTIHPDFVEILNYTKQH
                     FPQLNINLFSNFSFNNKKIIDALIANGVQLFISMDSPKADELSAIRKGANFQFIENNI
                     KQLQELPNRPFVIFTIQEANINRIFEMAEFAFENNCQILYNTIRRDEGVEVFIEIVKQ
                     NYQSILEQFKQIGKLYANSELQFFYPDQLAGVELQTEKPTKTHGTMKQCPALEKELCI
                     LYDGTATPCNMFNPYTYGNIFNQSLSEIWESKERKIFLSSHKCHYYCQNCANLGV"
     gene            complement(83974..84972)
                     /locus_tag="PLM04_00360"
     CDS             complement(83974..84972)
                     /locus_tag="PLM04_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01567.1"
                     /translation="MEILRNRLKLTLLITIFLFPTLIFGQNPKSIIKDFNNDGYKDTL
                     ETNYDGGSGFGGTYLSLINGKTKEIFELNDFGCFCDIKKIILIPPKLMQLRNKPFLEA
                     IKKELLPARRNFPDASLQWLISANINNKKLSNNSYYDLFIKTPPQWIPGKIELPNTYY
                     IDVSGDTLQKLYFADNEKPTWYNPKKNKGWLVYYAHNHYQNQKGNSLVLVDASTKYKV
                     FRTFHGVVVTKGNLHTWVFVTDYQLTGAPEKLRWKSIEDVVLIDKYLILQLINSKAFS
                     NPIFIIDIEKGFTGRLRCNDNFFKSYRIDKENFIVESNKATKTYKLQKLFSELSKQ"
     gene            complement(85198..85440)
                     /locus_tag="PLM04_00365"
     CDS             complement(85198..85440)
                     /locus_tag="PLM04_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HON01568.1"
                     /translation="MLIVAFKIFKTALAAANIKKKVSIHSLRHCFATHLLDNGTDLKY
                     IQSLLGHSSSKTTEIYTHITAKGFDQIKNPLDKLDI"
     gene            complement(85786..86019)
                     /locus_tag="PLM04_00370"
     CDS             complement(85786..86019)
                     /locus_tag="PLM04_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01569.1"
                     /translation="MYYKEESQSIGVSTFAITHELYIHLVFVNFVSHKPTPNPKIAKE
                     LPPLSPEGKIIFLKSLPSKIFRTASTQTKQTEI"
     gene            complement(86009..87055)
                     /locus_tag="PLM04_00375"
     CDS             complement(86009..87055)
                     /locus_tag="PLM04_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01570.1"
                     /translation="MKELSKNLIKINQQLKRIEIKEFEIENQIVFNYFDKLPESERGE
                     KLLKAIYIGVLAFMEDRISTFLSKTSNELGTELESLKMIFEMKKELFYKSTIKGSLAE
                     NEIAEFLNQYFADKKLNDKAFLTGNTAGKLPKNKTGDIICEVNGTPDLKIAIECKFDK
                     SVDLGDIQSKDIFTRKSDTAWSQLIEAQANRDSIVSLIVFDISLVHKSILNNFENVGY
                     IPGVGFVAIINSQKGDYSNLTIAYMLARDIALNAKEIELDKDILAIIVNRIIKDINEV
                     LTIKSLVQKNIDNNKEILKQLEKSILLMEFNQEYLKRFLREGTLTKKDLLDFYSGEDI
                     KDKYRLIEKEINVL"
     gene            complement(87039..87260)
                     /locus_tag="PLM04_00380"
     CDS             complement(87039..87260)
                     /locus_tag="PLM04_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HON01571.1"
                     /translation="MSVPEMVPVSSSNIESIGYDEQNEQVYVKFLNGSLYVYKDVPLH
                     EFENLRDAPSLGSYLHRNYKNVYPYERIE"
BASE COUNT        26672 a        15939 c        16846 g        28039 t
ORIGIN      
        1 taatttgtgg tgtaaaaatt tgaattttca ataagaaaat ctacagaatg actaaaaata
       61 cgtaaatttg cagaaagtgg ttatttttga atctcaatat tcttttcagc aattacttat
      121 tttaaacaca aaaaggcagc ctttttgctg ccctttgtgg gtggaggacg ggactcgaac
      181 ccgcgacatt cggaaccaca atccgacgct ctaccagctg agctacgacc accatttttt
      241 gcgagtgcaa agataacaca gcatttgatt ctgtgcaact attttaaaaa aaaattgacg
      301 tcttttggtt tattgccgat tttttggata aatgtttaaa tgaaatgaat aagcagacaa
      361 tcgaacaaaa caatgaggtt aaggagattt tacaaaaact ttggctttcc cgaaacggag
      421 aagtagcttc ccaaatgcga cagtacggga tggtgtaccg caaaaatctg ggcgtcgcga
      481 taacccgcat aagggaaata gcttccgatt atgagccaaa cgaagaaaca gcacaacaac
      541 tttggacact tcacggcaga gaaaccatgt tgatggcaat cctgcttcag cctgtaaaca
      601 attttagttg ggaaatggct caaagttggg ccgaagactg cgacaacata gaaacgattg
      661 aatttgcgtg catgcatctt ttctcaaaac tcccttttgt caacgaactt tgctgtgatt
      721 ggtgcgcttc ggacaaagtt caattgcaaa taatgggatt tattttatcg gcacgcaatt
      781 atcaaaattt ttcggatgtg gatagaaatc ttattattga aaaggctttt gaaaattcga
      841 caaccgataa tttccatctg tataaagcca tcagtttggc gctttgcaga ttttgcagaa
      901 atggaaaatc catcgcagat tttattctcg aaaaaatgag caacttcctt cttcaaccaa
      961 ctgccggtca acaatatatc atggaagaag tgaaatcgga aatgaatttt ttggaacttt
     1021 tataacaatt atattctaaa aaatcttacc tttgttctat ggatgaaaac aaagaaaaag
     1081 aagaaaattc ctttgaagtg gtcaagaaaa tttttaacga ttatttggct gcccatcagc
     1141 atcgcaaaac gcccgaacgt tacgcaattc tggagcaaat ttacacccac gaaggacatt
     1201 tcaatgccga gctgctttac aatgccatgc aaaaggatta tcgtgtcagt ttggccacta
     1261 tttacaatac gctcgatttg ctgctcgagt gcaaattaat tgtaaaacat caatttggcg
     1321 atcaactggc acaatatgaa aaaactttcg gcaccaacat tcatcatcat cttatttgta
     1381 cgcaatgtgg caaaataaag gaattttccg acaaaaatat aaaagcagcc atccagtcga
     1441 agcattttca aacttttcaa atgtcgcatt actcgctgta tatttatgga atttgcagta
     1501 aatgcctgaa aaaaaagaaa aaaacttcgt aaaaagtgtt gtttcaatga aaaaaattac
     1561 cttatttttc accttttctg taaatggatg ccgtaagcaa acggattctg aagcaaggaa
     1621 aggttttatt ttgcaaaatc ataaaaattt aaacgaatga aagcagatgt tttattaggc
     1681 cttcagtggg gcgacgaagg aaaaggaaaa gttgtggatg tactgacgcc acattacgat
     1741 attgtggctc gatttcaagg cggccccaat gccggtcata ctttggagtt taaaggaaaa
     1801 aaatttgtat tgcgttcggt tccgtcggga atttttcagg gcgacaaaat caacgtgatt
     1861 ggcaacggga tggtgattga ccctgccctt ttcaaggaag aagtggaagc tctcgaagca
     1921 gcaggcgaac ccttgcatca gcggctgaaa atttccaaaa aagctcatct cattcttccg
     1981 acgcatcgat tgctcgatct ggcttccgaa gctgccaaag gaacaggcaa aattggtaca
     2041 acgggcaaag gtattggccc tgcttataca gacaaaataa gcagaaatgg acttcggata
     2101 ggtgatattc tgcatcgttt cgacgaaaaa tatcgtgaag tcgttgctcg ccatacggaa
     2161 atgttgcgtc aatacaattt tgaatacgat ttggctgcat tggaaaaaac tttttttgaa
     2221 ggaatagaat gtatcaaacg ttttgatttg attgacagcg aatactttat caaccactcg
     2281 ctggaagaag ggaaaaaaat tttggccgaa ggtgctcagg gaacgttgct cgacattgat
     2341 ttcggttcgt atccttttgt aacttcttcc aacacgattt gtgccggcgc ttgcaccggg
     2401 cttggcctct ctccgcaaaa aatcggagaa gtttacggta ttttcaaagc ttattgcacg
     2461 cgcgtgggaa gcggtccttt tccaaccgag ttgaaagacg aaaccggaga actgattcgc
     2521 aaaaaaggtt gtgagtttgg tgctgtaacc ggccgtcctc gtcgttgtgg ttggatcgat
     2581 ttggttgcat tgcgctattc aatcatgctc aatggcgtta ctcagttgat tatgatgaaa
     2641 agcgacgtgc tggatgattt tgataccata aaggcttgtg tggcatatcg tatcgacgga
     2701 aaggaaacgg aagaatttcc atttgaaatt accgaaaatc tcgaacccgt ttatgttgaa
     2761 ttgcccggtt ggaaaaccaa aatatcgaac ttgcaaagtg aagacgaatt tcccgaagaa
     2821 ttgaatgcct atttgaattt tttggaagaa gaattgaatg tcccgataaa gattgtttct
     2881 gtaggacccg accgtgaaca aaccgtcatt cgctatcagg aatagttttc caatttttat
     2941 gcactacaaa ctgccaatct gtcaattaaa tttttggcaa acattttgca tgaaataaaa
     3001 tgttaattat aaaattgaaa agactatgat ttatattatt tttattgttt ttgcgctgat
     3061 tagttttttg cttcagaaca ggttgcagtc aaaattcaaa cgttactcga aattacctgt
     3121 accaaatggt atgacgggaa aagatgtagc cgaaaaaatg ctgcacgaca acggcattta
     3181 tgatgtgaag gttatttcca cctccggtca tctcaccgat cattataatc cggcaaacaa
     3241 aaccattaat ttgagcgaac aggtgtacgc ctcgagtaat gtggcggcgg cggccattgc
     3301 tgctcatgaa acagggcacg cggttcagca cgcttatgct tatgctccac tgactctgcg
     3361 ttcaaaactg gttccggtgg taagttttgc ttccatgtgg atgcaatggg tactgttggc
     3421 aggtattatt tttcttcaat cttttcctca actgatgttg acgggaatta ttctttttgc
     3481 gctgaccacg cttttcagtt tcataacttt accggtagaa atcaatgcca gtcaacgtgc
     3541 gttggcctgg ctcaacaatg ccggcattac caattatgaa acccacgaac cggccaaaga
     3601 tgctttgaaa acggcagctt acacttatgt ggtagccgct ttgggatcgt tggcaacgct
     3661 ggtttattat atcctgattt tcatgggtgg aagccgaaga taaatataaa aattcataca
     3721 ttttgataag gaaaaccgac ttttttaaga gaaggttttc cttatttctt ttttcaaatc
     3781 gggaaaaatc actacatttg catccgcatt ggctccgtag ttcagttgta tagaacgtca
     3841 gattccggtt ctgaaggtct tgggttagaa tcccaacggg gtcacacaca gccaacctta
     3901 acatgtttgt tcgggttggc ttttttggta agaaaatttt tatttgaaaa taattgtatg
     3961 ttcttcactt tcagccgatt ggctcaaagt tgttacactt ccaatttttt cgttcaaaat
     4021 cactaatttt gcagaaaaat tttaatattg aatcctgctc aatcaatgag aaaaaagaaa
     4081 cagctccccg ttttggaaaa ggtaaccatt accgatattg gagccgaagg caaagcgctg
     4141 acaaaaataa acgatatggt cgtttttgtt cctttggtgg tgccgggcga tgtagtggac
     4201 ttgcaagtta cgcaaaagaa aaaaaattac atggaagccc gagctgtaaa atttcatcac
     4261 tattcggaac aacgttgtga gcctttttgt caacattttg gcatttgcgg cggctgcaag
     4321 tggcaaatgt taccttatgc agaacagttg aaatacaaac aaaaacaggt agaagatcat
     4381 ttgaagcaca ttggaaaagt tcaattgccg gaaatttcgc ccattctccc ttccgaaaaa
     4441 accgtttttt acagaaataa acttgaattt accttttcca atcgccgttg gcgaagtttt
     4501 gaagaaatat cttctgcaca gaatattgaa gatgaaaatg cactcggatt tcacatcccg
     4561 ggacatttcg acaaagtgtt gaacattgaa aaatgctggt tgcaggacga tatttccaat
     4621 aaaatcagaa acgaaattcg tcgttatgct ttggagaatg aattggaatt ctttgatctg
     4681 cgccatcagg aaggattttt gcgaacaatg atggtaagaa cttccaccac tggtgaagtg
     4741 atgctggtag tcgttttttt tcgggaagac gaaccgaaac gaaaagctct tttgcaacac
     4801 atagccgaca cttttcctga aattacttcc cttttgtatg taatcaattc caaagccaac
     4861 gataccatta ccgatcagga aataatttcc ttcactggaa atgatagtat ttacgaaaaa
     4921 atggaagatt taactttcaa aattggggca aagtccttct ttcaaaccaa ttctgcacaa
     4981 gcttgcaggt tgtatgaaaa agcccgcgac tttgcagcat tgacgggaaa tgaacttgtt
     5041 tacgatttat acaccggtac gggcaccata gccaattttg tggcgcgaaa ggcaaagaaa
     5101 gtgattggaa tagaatatgt acctgaagca gtggaagatg ccgtgaataa ttcaaaactt
     5161 aacaacatta ccaatacatt tttttatgcg ggtgatctga aagaagtaat gacagccaat
     5221 tttattcgtg agcagggaaa tcctgatgtc attatcactg atccgccaag agccggaatg
     5281 catggggatg tagtggatgc cattctgaca accaatgccg caagaattgt ttatatcagt
     5341 tgcaattcgg caacacaagc ccgtgatttg caacttttgg acagcaaata taaagttact
     5401 gccgttcagc cggtagatat gtttcctcat acgcatcatg ttgaaaatgt agtattgctt
     5461 gaacatcggt aaacagaata ttttaaatgt atttgttttg tacgagcctt tggaatcttt
     5521 tgttatcatt ataaccaatt gttaatcagt tataaagata caaaatttca aggtgttcac
     5581 caaaaatgac aattatttta ttgattatca gataattata taactataat ataatttttc
     5641 tcatgtgcta aaatatattt gaaaaaactt atagcttaaa ttttaaatta tcatgattag
     5701 tttttttatt tccattggac ttttaatagc aggttatttt atttatggaa aaattgctga
     5761 acgtattttt ggcattgagc cgaacagacc tactcctgct cttgccaatc ctgatggcgt
     5821 agattatatt cctttgccat ggtggcgggt atttttgatt caatttttaa acattgctgg
     5881 tttaggacct atctttggtg caattatggg tattatgttt ggtgaatcag cttttttatg
     5941 gattgtattt ggaaccattt tcgctggtgg agttcatgat tatctttctg gtatgatgtc
     6001 cattcgtaac aatggtacaa gtttacctga attggtagga aatgaactgg gaaatggtat
     6061 caagcaattt atgagggttt tttcgctggc tttgatgata ctggtaggtg cagtttttgt
     6121 ttcaggtcct gcacaacttc tggcaaattt tactcctcat gctattaatg taagtggatg
     6181 gattgtaatc gtatttacct attatatttt agctactctg ttgcccattg ataaaattat
     6241 tgctaaaatt tatccatttt ttggttttgt cttattattt atggctgttg gaattttttt
     6301 agctcttata ctaaataaag ctcctatccc ggaatttaca gaaggatttg ccaatcttca
     6361 tcctaataag ttacctattt tccctattat gtttgtttcc attgcttgtg gtgctatttc
     6421 aggatttcat gcaacccaat ctccacttat ggctcgctgt attcaaaatg aaaaattagg
     6481 acgtcgtatt ttttatggca gcatggttgc cgaaggaata gtagctttaa tttgggctgc
     6541 agcagcttcc agtttttttg gttccattca aggattacaa gaatttctgg gaactcttcc
     6601 ggctactgct aataaacctg ctgtggtggt agatcgtatt tgtagagatt ggctgggaaa
     6661 tattggagga gttataacat tattaggtgt aatagctgct ccactcactt caggcgatac
     6721 ggcattgcgt tcagcgcgac tgattgtagc tgactttatt catctggagc aaaaatcttg
     6781 gaaaaaaagg ttgatgattt ctattcctat cttcattttg acttttattg tcctacaaat
     6841 taattttgat gctttgtgga gatattttgc ctggtgtaat cagacattgg ctgtttttac
     6901 actctggtct atcactgttt atctggcaaa aaggcacaag ggttattgga tttctcttat
     6961 cccagcagta tttatgactt gcgttagtgt aagttatatt tgcattgcac ctgaaggatt
     7021 tcaattacct cagtatcttt cttatcttat cagtgcaatt gcttgtgttg tggttatatt
     7081 tttcttttat cgttttattt accttaataa ggaaaaatat taactgaaaa tcaattattt
     7141 aataaaaaat gattcaaaaa agttcaaaat tgattctttc ccgttgaaaa ttgactaatt
     7201 ttacattttt ttttaacaga taaaaagtac tttctaaaaa gcagtattac tgtaatttct
     7261 tatttttggc aaagaatgaa aaaagacatt ctgatgaaaa agcaaataaa atattttttt
     7321 taaaaaccta aacggatatg gcgataactg ttcgagaagt aactaccaaa aaacaattga
     7381 aacaatttgt ctggtttggg atcaatttgt acaaggataa tgaatatgca gctccaccgc
     7441 tgtttataga cgactatacc aacctgacta aaggaaaaaa cccttcgctt gatttttgcg
     7501 aagctgctta ttttcttgcc tacaacgaaa aaaatgaaat tgttggtcgc attgcagcca
     7561 taatcaatcc ggttgccaat gaaacatgga atcagaaaaa tgcacgtttc gggtgggtgg
     7621 attttatcga cgactatgaa gtttcgaaag ccctgtttga tgcggcagtg gaatgggcaa
     7681 gagccaaagg catgacagcc atgcaaggcc cactcggatt taccgatttt gaccacgaag
     7741 gtacgcttat cgaaggtttt gaccgacttg gaacattggc cggcatatat aattatcctt
     7801 attattcaca gcacatcgaa aaatatggtt ttgtgaaaga tgccgattgg gttgaatttc
     7861 tcattaccat tcccgaaaaa tttcccgata aatatttccg tatcgctgag ctggtgaaac
     7921 aaaaatatca cttaacacct cgacatttta aaagccgaaa aaaactggta aacggctatg
     7981 gaaacaaaat ttttgatttg ctcaatactt gctaccagca tttatacggt ttcaccaaat
     8041 tgaacgaagc ccaaaagcaa ttttacataa aactctattt cagctttttc aggctggata
     8101 cggtttcggt ggtaaccgac gaaaatgatg atgtagtagc agtgggtatt gccatgccaa
     8161 gtctgaccaa agctttgcaa aaagcaaaag ggaaactatt cccgctgggt tggttttatt
     8221 tgctgaaatc tatttattat aaaaacgata tcgtggattt gtatctgata gcagtacatc
     8281 ctcgttatca gggaaaaggt gtcaattcta tcctttttgc cgaattgatg gtcaattttg
     8341 caaaaaatgg atataaattt gcagaaagta atccggagtt ggaaaccaat actcaggttt
     8401 cctctcagtg gagtgatttt gaacatatca atcacaagcg cagaagagct tacattaaac
     8461 aaatctgaaa atttactttt tcttgaaaaa attatttaat tcgttttttt gaccaactat
     8521 gaaatttttt gaatggttgg actccaccta tcaacttctt tcaacttttt tagctcgaat
     8581 tttgtttgaa tagtgtgaag tcaataaaca attgacttaa tacatattga ttatgacacg
     8641 aaaatttgat tttttggtaa taggaggtgg aatagctggc atcagttatg cactcaaagt
     8701 agctgaatac ggaaaagttg ccctcatttg taaaaccaca cttgaagaat cgaatacttc
     8761 tttggctcag ggaggtatgg cagccgttct ttatccaccc gacaattttg aaaaacatat
     8821 tcaggatact ttaattgcag gtgattacat aaataatatc gaagctgtaa aaaaagttgt
     8881 ttacgaagcc cccaaacaaa ttgaagaatt gattcgctgg ggtgtgaaat tcgataaggc
     8941 ggaagacgga aaattcgatt tgcatcgcga aggcggtcat tcggaatttc gtattctcca
     9001 ccataaggac aatacaggag cggaagttca aaaaagattg aacaaaaaag ttctacacca
     9061 tcccaatatc gaaatttttg aatatcattt tgctctcgaa attttaactc agcatcattt
     9121 gggtcaaatt gttacgcacc atactccaaa tatcgaatgt tatggcgctt acattcttga
     9181 ccaaaaaaac aatcaaatta ttacttttct ttcaaaggtt accatgttgg caaccggagg
     9241 tattggaggt gcatatctga cttctaccaa tccgcccact gccactggcg atggcattgc
     9301 aatggtacac agagccagag gcgtaataaa agacatggaa tttgtgcagt ttcatcccac
     9361 tgcactttac catccgggtg ataaaccctc tttcctgatt tcggaagcca tacgtggcta
     9421 tggagcgatt ttgcgtacca aagacggaca aaaattcatg cacaagtatg acgaaagaaa
     9481 agagctggct ccccgagaca ttgtagcccg cgctatcgac aacgaaatga aaatacgcgg
     9541 tgacgaatat gtttatttgg atgttacgca caaagaccca aaagaaacaa aaaaacattt
     9601 ccccaccatt tataaaagat gccgcgagtt gggtatcaac attaccaaag attatattcc
     9661 cgtagctcca acacagcatt atttgtgtgg tggcattgct gtggatttga acgccgaatc
     9721 aaccattcat cgtctgtatg cggcaggaga atgttcctgt accggtttac atggagccaa
     9781 ccgtttggct tccaattctt tgatcgaggc aattgtttat gctgatgctg cagccaagca
     9841 ttctgttgtc cgacttaatt cacttggttt tcaggaaaat attcccgatt gggatgatga
     9901 aggcacaact tggcctgaag aaatgatttt gattactcaa agtgcgcggg aagtagaaca
     9961 aatcatgaat aactatgtag gaattgttcg ttccaatttg cgtttgaagc gggctatgag
    10021 ccgattggaa attcttttca gggaaacgga ggatttatat gagcgatcgg tggtttcgcg
    10081 ccaaatttgc gaacttcgaa acattatcag cgtttcgtac ttgattatca agcaggctac
    10141 acagcgaaaa gaaagtcgcg gattgcatta caacatcgat tatccgggac atgcagaaga
    10201 tttaaaaaat aaggaataac gacctgaata tttttcgata acgaatcaca aaataaactg
    10261 aaagtttatg aaccggcgat aaattttttc ccttttttct ttggacgttt ttcactattt
    10321 ttgcagtaaa ttatttctca acctgttaaa ttttctattt gcgatgaaac gtacgatgtt
    10381 gattgtgttt tggcttgctt tggccacgat gcttttagcc ggaagctatt ctcccgacga
    10441 tccgcgttca tcagcccaac aagtcgttgt taaatctgat gttgctgtgg gacaaaactg
    10501 gttgagacag gataactctt ccggtgcatc tgacaatcaa aatcaaaata tcgacaatgt
    10561 aagttttagc gctacatccg aaggaattta tttgtcattg ggcagtggaa catataatgt
    10621 gaaacttttt gcactcaccg gacagttgtt gtggagtggc gatttggttc aggggaaatt
    10681 ttttattccg gtgcgaaaag gaatttattt tttaagaatc aacgggaaaa gttttaaagt
    10741 ggtttgcaaa taaaactaat acttttgccc ccattaaaag aaaccggaaa aaactctatc
    10801 cgataaatta taaagaaatt ttttatggtt aaacacatcg ttatgtttca gctttccgat
    10861 ttttcggaag gaaagacaaa aaaagaaaat gcaacgctca tcaagtcgca attggaaaat
    10921 cttcaacact tgatacctga attgaaaaaa atcgaggtcg gtattcaaat ggaagaaacg
    10981 gctgcagaaa attatgattt ggtattgtac accgaatttg aaaatgtaga ggatctgcaa
    11041 atttatatca accatcccga gcacaagaaa gtcagcgcat atatcggtaa aattaaaacc
    11101 ggcagagtag cagtagacta caaagtttga tcaaaaaaaa acaggaaaaa gaaaagattt
    11161 ttctttatca caagcaaagg tgaaaagaaa aatctttttt tatgtatcgg tttgttctgc
    11221 gttaacggag tttagcaccc agtttttcgg tgaaattttt cagaagtttt tgcataatgg
    11281 catcaatctg actgtcggta agtgtttttg tttcatcctg caataaaaag ctcactgcat
    11341 aagatttttt acctgcttcg atatttttgc cttcgtacac gtcgaacaac tgaacttttt
    11401 tcaaaagttt tctttcggtt tgataggcta ttttctcaat ttcggcaaat gttacgcttt
    11461 tatcgagcaa cagggccaaa tcacgtttta cttcaggaaa ttttgatatt tcggcaaaac
    11521 taacctgttt ccctttcgtt tcttctatca acaaattcca atcaatatcg gcataaaaaa
    11581 cttcattttc aatatcaaaa cttttcagca gtgcagcatt cactattccg tacaatgcca
    11641 gttgtttacc ggctgcattg cgaaagctca aagcttgtga aaacaaatcg ttagaaatct
    11701 cctcgaagtg aaaatgctgc aatttcaatc ccagcctacg aaaaatattt tccacgtatg
    11761 ctttcagttc aaaaatactt gatttttcct ccgaagaagt ccacgattgc ggatgtttgt
    11821 tgcccgacaa ccaaagtccc aaatgaaatt cttccaaata tgccgataaa ggatcggatg
    11881 aatttttatt ttcgggaaga tagaaatagc aattcccaaa ttcataaaac tttatgtcga
    11941 aatttttccg attgttgttg taaacaatgt tttccaaacc tccaaaaagc agagtttgtc
    12001 tcatcacgct caagtccgaa ctcaacgggt tcattatttt tacacatttt tctaaaggca
    12061 aagttgcaga aagtgcgtaa taatttcttt tggtcagcga attgttcatt acttcattaa
    12121 aaccctgagc tgtcagttgc tcggaaataa tgttctgaat acggaaattg ttcggtttag
    12181 gtcgatagct caacgtggaa accagttttt ctccgatttc tacattgttg taaccataaa
    12241 tacgaagaat atcttcaatg acatccacat cgcgttgcac atccaccctg taggtcggga
    12301 ctttcaaaac aaaaccttct tcggaagaag attttatctc aatttccaaa gccgaaagaa
    12361 tggtctgaat ggtgtccttt tcaaaatttt ttcctatcaa agcgttgatt ttcgcaatgg
    12421 tgatgtccac ctcgaaagga agtatttttt tggggtaaac atcaacaatt tctcctgcga
    12481 tttctcctcc ggcaatttcc tgaatcagca aagcacagcg cttcaaagcg taaatggtgt
    12541 tgttggggtc gcagcctcgc tcaaagcgga aagaagcatc ggtactcaat ccgtgccgac
    12601 gagccgtttt tcggatataa acgggattga aataggcact ttccagaaaa acgttttgtg
    12661 ttgattctgt aactcccgaa tcgagtccac caaaaacgcc tccgatacac atgggctctt
    12721 ctgcattgca aatcatcaaa tcttcggcgc tcaatttacg ctctactcca tccagcgtta
    12781 caaaagtcgt tccttcaggc atgtgtcgca caataacttt tccgcctttt attttatcgg
    12841 catcaaaaga atgaagcggc tgccccaatt catacaacac aaaattggtg gcatccacca
    12901 cgttgttgat gggacgcaag ccaatggttg tcaggtaatt tttcagccat ttgggcgatt
    12961 cgccaacctt tacgccggta atggttacag aagaatatcg cggacaagct tccggatttt
    13021 ctactgttac cggaatggta agcccatgtt gttgaacctg aaaattatct accgacggca
    13081 gggtaagttt tatcgatttg tctcttaacg aaaaataggc agccaaatca cgagcaacgc
    13141 cgaaatgtga agcagcatcc acgcggttgg gtgtcagatc tatttccatc acataatcgc
    13201 tttttatgcc gtaataatct tttgccaaca ttccgggttt tgcatcgtcg ggcaaaacta
    13261 tgatgccttc gtgactcgtt ccaattccta tttcatcttc ggcacaaatc attccgaaag
    13321 attccacacc gcgaattttg gagcgtttta tggtaaaact ttcgtcgccg gcatacagtt
    13381 ttgtaccaat ggtagccaca accacttttt gtccggaagc cacattcgga gcgccgcaaa
    13441 caatctgcaa aggttcaccg gagccaatat tgacagttgt tacgtgcaaa tgatccgaat
    13501 tttcgtgagg aacgcaagta agcacttcac caataaccaa cccttccaaa ccgcctttca
    13561 cagtttgaac ttcttcggtg ctgccaactt ccaatccgat cgaagtcaga atttccgaaa
    13621 ccttttctac cggcaaatcg atgttgatgt attttttgag ccaattatac gagatattca
    13681 tatttgtttg tttttatcgt caattatttg gaaagggtct tgatttttcc gctgcaaaag
    13741 tacatgaaaa tcgaggtatt tccaacttcc aactataaaa tcttgaacaa aaccttaata
    13801 gcttgccata ggataatagt tgaacagttt ctattgatag gttgcattta atcaattaca
    13861 gaaggataca gctacggaat atagaaagaa aaaagttttt gaagaattag ttacttgtat
    13921 tttttaaaag aaaaatatca agatttttcc tttgaaattt tttgcaaatt agcaaaaaga
    13981 aagtatcttt gctgcgcttt tccaaaagat caaattgtgc gaaaatagct cagttggtag
    14041 agcacgacct tgccaaggtc ggggtcgcgg gttcgagtcc cgtttttcgc tcaaaactta
    14101 tccctgcaat tttgaaaaca ggaatagccc aaatggcgga attggtagac gcgctagttt
    14161 cagggactag tggccgttag gctgtgcagg ttcgagtcct gttttgggca caccaagttt
    14221 tcattttgtt ttttgcgcag atggcggaat tggtagacgc gctactttga gggggtagtg
    14281 ccggttacgg cgtgtgagtt cgagtctcat tctgcgcacc ctgagtaaat cttgtttatc
    14341 ttctcttttg gtctttttct tcgttaaatg gttcggtatg aatattaatg atggtttgtt
    14401 taccaaatcg atttcttagc ttattttcaa tttcagttgc aatatcgtga gaacgtaaaa
    14461 aggttgtagt tttgtcgagt ttaatgtgta tatcgatggc aaaagtgttt cctacttttc
    14521 tggttttgag gttatggtaa gacagtattt cagggttcga ttcgataatt tcaaatattt
    14581 cctttttcgt ttcttctggt aatgaccttt ctaaaagttc attgacgctg ggtaataaaa
    14641 ttttcagggc tactcctatt ataaaaaagc tgacaatgat acccgctatc ggatctaaaa
    14701 ttcgagcctg ctcaccaaaa aaaattgctc cggcaatacc taatccggta cctattgatg
    14761 ataaggcatc ggagcgatgg tgccatgcat ttgctacaat ggcttgattg ttaatttttt
    14821 tcccaattct tgctgtatat cggtataatc cttccttaac aataatactc accagagctg
    14881 ctatcaatgc tataatggtt ggtggttcaa ttatttctcc ctgtgttacc tgtataactt
    14941 tgtttagacc gtttatgaaa atgtaaactc ctacagcagc gagtgcaagg ctgaccagaa
    15001 aagttgcaaa tgtttcaaat tttccgtgtc cgtattcatg gtcttgatct ttttcttttc
    15061 cagcaattct tacaaatata ataacaatca aatctgttat aaaatcggat agtgagtgta
    15121 ctccatctgc aatcatggca gtacttttac ccaaaaaacc tgccaaaagt ttaaatactg
    15181 ttaaggtaaa attaacccaa aaacccatcc acgtagcatg catggcttgt gatgttctgt
    15241 cttcattttt catatttcac ttcaatgata gaaaaatatt catgaatgca aaaataataa
    15301 ataataaatg gttttttcac ctatttttga agtaacaagc taatttataa gaagtttaaa
    15361 taatatttta aactattcta aattacttta ttccagtcaa aattcactta aaagcagtaa
    15421 tcaaggaatc aagtaagaaa attctttttc aacagaatat aatttatttt taaaggtctg
    15481 atggaaccca catgtctgaa tattatattc attcttgttt gtgtatgcaa tacatgtggg
    15541 tttcataaac aactctgtaa aacctcacag ctatttggtt ttctacaaag ttaaaacttt
    15601 ttcttttagg aatttttgtt ttgatttacc aaaaatgagt ttttcaaaca ttttatctaa
    15661 tttcgcattt gtcggttgaa ttttcctcgt cataaggctg actctatatt tttcctattt
    15721 ggagaattta ttttatcttt gtagttcgat tttttatttt tgaaatcact atcattacaa
    15781 actgagaagt taaaattgac taaaaatgtt ccgtaaccaa aagataacag acgatatata
    15841 ttatgtaggc gttaacgata gacagaaagc gaaattcgag aatttaattc ccttatacga
    15901 tggtgtttct tacaatgcat atttgattgt agatgaaaaa accgctttga ttgatacggt
    15961 agatattcat tttggcgaaa tattgataca aaaaatactc tcacaattgg gcgataaagg
    16021 attagactat ctgattatca atcacatgga accagatcat tcgggttcct tgcggttgtt
    16081 gagaaaatat tttccaaata tgactattgt aggcaatgaa agaacattga gcatggtgaa
    16141 aggattttat gaaattgatg gatcgctttt gaaagtttcc gatgaagaaa gcctttcttt
    16201 agggcgccat caattacagt tttttctgac tccgatggtt cattggccag aaacaatgat
    16261 gacttttgat catgccgata aggtgctttt ttcaggcgat gcttttggaa gctttggtgc
    16321 acttaatgga agtatattgg atgaaaacct tgatatagac aaatattggg atgaaatgta
    16381 tcggtattat gctaatgtta ttggtaaata tagttcgccg gtacaaaaag ccataaaaaa
    16441 attggagcat ttaccttatg aaataatttg cagcacacac ggccccgtgt ggaaaatcaa
    16501 tctaaaaaaa gctgtagaaa tatatgatag actaagcagt ttccaagcag aagaaggagt
    16561 agtgatagtt tatggatcaa tgtatggaaa tacagaacta atggctgaaa cggtagcgca
    16621 aggtttggca gaagcaggtg ttaaacccgt gattattcac aatatttcaa cagtcgatct
    16681 ttccatcatt ttgcgagaca tttaccgata tagaggttgt atttttggga gtccaactta
    16741 taacaacgga ctatttcctg aaatggaaga tttaattaga aaaattgaaa gtcgtggatt
    16801 aaaaaacagg atattcggtt gttttggttc ctatacgtgg tcgggcgcag tggtgagaaa
    16861 cttgactgaa tttgcaaatc gcttgcaatg gcctatgtct gatgtgatgg tggaagaaaa
    16921 acagggtcta aaaaaagata attatgaatc ctgcctacaa ttaggtcatc aaatagcaga
    16981 aatgttacaa aatcctgttc aattcaataa gggctgttaa ccgcgtaact ttttaaaaaa
    17041 ggtaatatgt ttcttcctgt gtatatttta acttactaaa ttttttaacg taatggaaaa
    17101 tagacattca tccggacttg gcaaaataaa tttggtgctt atagcatccg gatttgcaat
    17161 tattgttata ggtttttttc taatgacagg ttcttctacc caagtggaat ttaatccgga
    17221 tattttcagt tttcgtcgca taactgtagg cccaatggtt tcattttttg gttttttgtt
    17281 tatcatattt gccattcttt ttaaaccaaa aagtaaataa tgatgacttt ttggcagaca
    17341 attataatcg ccattgttga aggactaacg gaatttcttc ctgtttcttc aactggacac
    17401 atgataatta cacaaagttt tctgggtata gaaagtacgg aatttgtaaa agctttcact
    17461 gttattattc aatttggagc tattctttct gtgattgttc tttattggaa cagatttttt
    17521 cggttgcaac cactggatga atttaatgtg ccgacctatt ctgaagggaa aaaatttgtg
    17581 aaacaacgtg ttattatccg aaaatttttt tatcgatacg atttttactg gaaactgtta
    17641 attggttttt tacctgctgc tgtcataggt tttttagcag gcgattttat tgatgttttg
    17701 ctcggagatg tttttgttgt tgctttgatg ctctttatag gaggctttgt gatgcttttt
    17761 gtagataaat ggttttcagc ctcttctcaa gaccagtcaa ttggttggca aagagcttta
    17821 aaaatcggtt tttttcaatg tatagccatg attcctggag tttcccgttc aatggccacc
    17881 atagtaggtg gaatggcggt aaaattaacg cgaaagaatg cggcagaatt ttcgtttttc
    17941 cttgctgttc ctactatggc tgcagcttca gggtacaaac tttttcagct tctcagcgaa
    18001 cagggtggaa aggatttgct ggtgcagaac tggttgttgc tgttattggg taatgtaata
    18061 gcttttattg ttgcattggt tgccattcgt ttttttatcg attttataag tcgttatgga
    18121 ttcaaggcat tcggttatta tcgcatagca gtaggaggag ttattttatt gttgttggga
    18181 atgggttata atttaaatat gatgtaaatg gatgttttca aaggcgaagt tttttatgtg
    18241 gataaacctt tgcacttgac atcattcaat gttgttaata ggttacgctg gaaaattaaa
    18301 aaagttgccg gaataaaaaa aattaaagtt ggccatgccg gcacgcttga tccggaagca
    18361 acgggcgtga tgattatttg tactggtaag gcaaccaaac aaattgaaag ttttcagtcc
    18421 caaaccaaag aatatattgc cgaattaaaa ttaggtgcaa ctacaccatc cttcgatatg
    18481 gaacttccag taaatgcggt ttatccaacc gaacatatca cgcgagaatt gatagatcaa
    18541 gttgtgtcaa cctttattgg tgaaatagaa caaatccctc cgatattttc agcggtgaaa
    18601 atagatggaa aaagagctta tgaatatgca agaaatggac aggaaattga actaaaacca
    18661 aaaaaattgg ttatagatga gattgaaata ctcaattttg ttcctcctac attaacgctt
    18721 cgaattgttt gcagtaaagg aacttatatt agggctttag ctcgcgatat tggttttgct
    18781 cttaactcgg gatcctttct tacttccttg agacgcatac gcattggaaa tgttcgaatc
    18841 gatgattgtt gggacatcga aaatttgttg aaccatttgg ataaagaact aaaatcgaca
    18901 gaataaaaat tcttacatta taaggctttt ggaacaactt taaaaattaa atctttagct
    18961 gataaatatg aaattatctc aatttaaatt taaattaccc gatgaattga ttgcccagta
    19021 tcctcctaaa catcgagatg aggcacgatt aatggtagtt cacaaaaaaa ccggagaaat
    19081 tgaacatcgt atttttaaag atgtgttgga ctatttcaac gaaaatgatc tctttatttt
    19141 caacgatacg atggtttttc ccgcccgtct ttatggaaat aaagaaaaaa caggagctca
    19201 aattgaggtt tttttgcttc gtgagttaaa tgctgaaatg cgtttgtggg atgttttggt
    19261 agaacccgcc cgtaaaattc gaataggaaa taaactttat tttggggaag atgattccat
    19321 tgtggcggaa gttatcgata atactacttc acgcggtcga accctacgtt ttttgtttga
    19381 tggcacacac gaagaattta aaaaagcgct ttatgcctta ggagaaacac ctcttcctca
    19441 ttatatcaag cgacaagttg aagctgccga tgcagaaaat ttccaaacaa tttttgccaa
    19501 aaatgaagga gcagttgccg ttcctgcggc aggtttgcat tttagtagag aattgttaaa
    19561 aaggatggaa attaagggaa tagatatggc ttttgttact tctcacatga gcttgggtat
    19621 atttcgagac attgacgttg aagatttgac caagcataaa atggattccg agcaaattat
    19681 tgttcctgaa gaaacggcca aaaaagtcaa caaagctcaa gaggaaggaa gaaatatctg
    19741 tgcagtaggg gttacggtaa tgcgagcact ggaaactgta gccggaacag aaggaaaaat
    19801 taaatccttt gatggctgga caaataagtt tatatttcct ccctatgatt tttcagttgc
    19861 taatgcaatg attaccaact ttcagtttcc atattccact ttgctcatgc taacagctgc
    19921 ttttggcgga tatgatacca ttatgcatgc ctatgaggtt gctattgaag aaaaatatcg
    19981 atttggagta tacggagatg ctatgttaat tgtttaatta ttgacttcct actcaatgtc
    20041 atatttcatt gacaggaaaa ttttcaatac gataaaaaaa caacatttca tttttatcgg
    20101 gtaaattcat taattttgga aatcggttga aatggtaaat ttcatttatt cagtaccgaa
    20161 taattttata tttgttaatt tgcaaattta aaacaacaca tgcctcacac ttcacaacgt
    20221 gggcaactca tgcccgaatc tcctatccgc aagcttgttc ctttagccga taaagccaaa
    20281 gcacgcggag taaaagttta tcatttgaac attggccagc ccgatttgaa aacgccacaa
    20341 gttgctttag acactttaaa aaacatagat cttaaaattt tggaatacag ccctagcgat
    20401 ggttttaaaa gtttacgcag caaattggcc ggttattacg acaagttcaa cattgaagcg
    20461 acggaagaag atattattat cactaccgga ggttcggagg cggtaaattt tgcctttatg
    20521 agttgcctcg atccgggcga cgaaattatt gtaccggagc ctgcttatgc caattacacg
    20581 gcttttgcca ttgctgccgg agtggtggta cgtccggttg tggcctccat tgaagatggc
    20641 tttgctctgc cacctattga gaaatttgaa gaactgataa ctccaaaaac caaaggaatt
    20701 ttaatttgca atcccaataa tcctaccggc tatctttaca cgcgtgctga aatgaataaa
    20761 attcgtgatt tggtgaagaa atacgatttg tatctctttt ccgacgaagt ttatcgtgaa
    20821 ttctgttata cgggtggtcc ttatatttcg gcttttcatc tcgatggcat tgacgaaaat
    20881 gtgatattgg tcgattcttt ttccaaacgt tacagtgaat gtggtatccg tgttggtgca
    20941 ctggtaacga aaaacaagga agtgagaaag aatgtgatga aattttgcca ggctcgtctc
    21001 agccctccgc ttataggtca aattattgcc gaagcttcta tcgacacgcc tgctgaatac
    21061 atgctcgaaa tgtacaatga atatctcgaa cggagaaagt ttttgatcga cggattgaat
    21121 cgtatttcgg gagtttattc tccaattccg atgggtgctt tttatacagt cgctcgctta
    21181 ccggttgatg atgccgataa attttgtgca tggctgcttt ccgatttcga ctacgaaggt
    21241 tctaccgtga tgctggctcc aggctccggt ttttatacca tcgaagggat ggggaaaaat
    21301 gaagttcgta tcgcttacgt gctggaaaaa gaagatttga aaaaagctct tgtcgttctc
    21361 gaaaaagctt tacaagccta tcccggaaaa acaatctgat gttggcagat gaaaaagatt
    21421 tgaacatttc tctgaaaatt caaaatattt ttgttcatct taatcgataa ttcttattga
    21481 tttgaatgtt gaatatttca tagcaaagcg aattcatttt cagcagggga agaaaaatgt
    21541 ttcccgccct gcggtgcgta ttgcaaccat cggtattgcg ttgggattgg ccgtaatgtt
    21601 gatttcggtt tgtgtggtga ttggtttcaa aagccaaatc gagaacaaaa tcatcggttt
    21661 tggaggacac attcaaatca ccaatttcga caacaacaat acctacgaaa tgaatcccat
    21721 aaaaatggat gctgctctga tacaaaagat tcggcaaatt tcgggtgtag ttcacgtgca
    21781 gcgttttgcc accaaaccgg gtattatcaa aaccgataat gaatttcagg gcattattct
    21841 aaaaggtatt gacaaggatt ttgactggaa ttttttccgt tccaatttgc tggaaggaaa
    21901 tattttaaat gttggcagcg atacgctgaa aaacgaaatt ttgctttcta aaaaattgtc
    21961 cgatttactg ggattgaaat tgggcgacga gctttttatg tatttcattc aggatcaagt
    22021 tagagctcga aaatttaaaa tcgtcggcat atattccacc aattttgttg attacgatca
    22081 gctctttgtg cttaccgaca tccgacatgt tcagcggttg aacaattggg acagtgcatc
    22141 gttcggtgga cttgaggtgt tgatagacaa ttttgacaaa atagatgaac ggggagaagc
    22201 tgtttatgaa gccacagcca atattttcag caaagatggc aatgcttact acacccaaac
    22261 cattaagcag ttgaatcctc agattttcag ttggctcgat ttattggata tgaacgtttg
    22321 ggtaattttg ctattgatgc tttccgttgc gggttttaac atgatttccg gtttgctgat
    22381 tttaattctt gaacgtacca atatgattgg cattcttaaa tcgttgggtg caaccaactg
    22441 gtcggtaaga aagatttttc tttatcattc ttttttcctg attgcaaaag gaatgttttg
    22501 gggtaacctc attggccttt ccatttgtgc tcttcagtat tttacgggta ttattccgct
    22561 cgatcccgaa gcttattatg tggcttccgt accggttgct tttccgtggg gttatattgt
    22621 attgctgaat atcggaactt ttgtggcatc aattttaatg atgattgcgc catcttatct
    22681 tatcaccaaa atttctccag caaaaattat tcgttacgaa taggtgcatt ttctattaac
    22741 aaccgaaaaa ttattgcttt tcaaattaag tggtaataac cacatacatt ttaatggatt
    22801 gccaaattaa tttctaaatt aatacgccta atagtagtcg aacaaaatca tttttaatgg
    22861 tttttccttc tcaaaaaaat tctagttttt taggggcttt tttacagcca ttttttcttt
    22921 ttgaaatacc acaacattac tccggcaatg agtagcatga cggcccacat ggcaaagtag
    22981 ccatattgcc agtggatttc aggaaaataa tcaaaatttg tcccgtacac tccaacaatg
    23041 aaagtaagtg gaatgaacag taccgaaaaa atggtcagta ttttgattat atcattcaaa
    23101 cgggagctta gggtattgtg ataaatgctc aactggtcgt atagaatttc gcggtagcta
    23161 tccattaatt cgcttacttc attaagattt acctgtaact ccttgaaata gggatggttg
    23221 tcatcccgaa taaattccga ttcgagtttg gacaggttca gaatcatttc tttggccggc
    23281 ataatatatt ttctcaaata attcaattct cttttatacc gatttatcag cgaaggaata
    23341 tcgttatcca attctttggt catgagttct tccacatctt caattttatc tcccaattgt
    23401 ccgataaggt acaaataatt atcaattacc acatcgagca atgcacaagc caaataatcg
    23461 atgccggcag tacgaattcg atgatgatgt tttcgtaagc gttcgcgaat gggatcaaag
    23521 gcatcgccct gattttcctg aaatagtatc aaggtgtttt tggtgagtat aaaactcaaa
    23581 ttttccacgt ccaatttgtc aagcttttca ttataagtaa gcattttgat tacgataaaa
    23641 agcccttttt cgtagtcctg aacttgaggg cgcgtcatgg gatccatgat gttcgacatg
    23701 atattgaagg gaatatcgaa agattcggca attttttcca tcagttttac ttcatggagt
    23761 ccttcaacgt ttaaccagtg aaatttatct ttgctatgat tgaaattcaa ttcgcgtaaa
    23821 gatttaattt ctgtttccac cacgctgttt tcatcatagg tcatcaaata cagacgtggt
    23881 ttttccattt ttttttcgcc acgaaaaatc aattcaaaag gagagacgcc aatatctttt
    23941 ttcctttttt tgatatattt tgccatatga cttatttttt tagttcaata acaaagatat
    24001 acgataattt tgaattctga tgcagctttt tcaattttga tgataaagag aagacgaaca
    24061 aaaaaaaatt gttaaatgag taacaggttt tgcagagaaa tccgtctttt tctctgttat
    24121 ctttatttga aaatagtgaa gacattaaat attgaaatga aacgttaact tctcgtgttt
    24181 gtcggttttt cattattgca ttttttctta tttttgcata aaaatcttat catgaattta
    24241 gatgtttcgg tgattggcta tttggcgggg ttttgtactg ctgtggcaca gtttccacaa
    24301 gcatacaaag tttacagaac caaagataca cgaagtattt cgctgggtat gtacgcaatt
    24361 atgacttttg gcgttttcct gtggctaatt tacggaattt tgattcagga ctggccaatg
    24421 atgttggcaa acggaattgg gttaattccc tgtacattca atttaatcat aacctttcag
    24481 aacgtttttc gtgaacgaaa gaaaatgata taaattggag taattaaaaa aattatcagt
    24541 ttaggttttc gaaactgaga aaaaatttaa cgtttcatta tcaatacgct atgaaacata
    24601 ttcaaattat caatgggcct aatctcaatc ttttgggaga aagagagcct tccatttatg
    24661 gaaacaaaag ttttaacggt ttttttgaag agttgaaaaa gcaatatccc gatgtgaaat
    24721 tggaatattt ccaatccaac aatgaaggtg aaattatcga taagttgcaa gaaattggat
    24781 tttcggctga cggaataatt ttaaatgcag gagcttatac gcatacttct attgcaattg
    24841 ccgatgccat tcgctccatt caatcgcctg tgatcgaagt gcacatttcc aatgtttttc
    24901 agcgcgaaag ttttcgtcat cattcttttt tgtccgaagt atgtaaaggc tgcattatcg
    24961 gattcggact gaactcttat cgactggctc tagaagcttt gctgcttatt taaaacggat
    25021 taggaagctg gttgaatttc aaatttattc attaattttt tatgccaaaa tttacaaaaa
    25081 taatagctac cattagcgat aaaagatgcg atcccgattt tattcgcgat ttgtttgaag
    25141 cagggatgaa tgtggttcgg atgaattcgg cacatctcaa cagggaagga tttctgaaaa
    25201 ttatcaatag tgttcgttcg gtgagcaacg aaattgccct gttgatcgat accaaaggtc
    25261 ctgaagtgcg aacgaccatt gccgaaaacg ataaaataat tttacatgcc ggcgacacca
    25321 ttaaaattat tggcgatcca gacaaaatct cgaccaatga atgtgttgcg gtcagttatc
    25381 cgttttttgt gcgcgattta catgtaggcg atgatattct tttcgacgat ggagaaatcg
    25441 agttgaaagt ggcagggaaa gatgagcagt atctttattg taatgtgttg aatgatggtg
    25501 tattgggaag taaaaaaagt gtgaatgttc ccggtgtacg catcaatctg ccatccttgt
    25561 cagcacgcga caaggaaaat attttatttg ccattgaaaa caatcttgat tttatagctc
    25621 attcatttgt tcgaaacaag caggatattc tcgatattca gcaaattctg gatcaacaca
    25681 acagtccgat caaaattatt tcgaaaatcg aaaatcagga aggcgtggac aatatagaag
    25741 aaattcttga atacacctat ggggtaatga tagctcgcgg cgatttggga atagaagtgc
    25801 ctcaggaaaa aattccgggt attcagcgcc ttctgatcag aaaatgtgtt atggcaaaaa
    25861 aaccggttat tgtggctact cagatgcttc attccatgat taccaatcca cgtcccacgc
    25921 gggcagaagt tacggatgtg gccaacgcca tttattatcg caccgatgcc ttgatgttga
    25981 gcggagaaac tgcttatggc aagtatccgg ttgaatcggt acgaaccatg tcgaaaattg
    26041 ccgaagaagc cgaaaaaacg aaactgacgg aaaacgatat tccgattcct acggcaacag
    26101 tcgatattac ttcttttctg gcaaatgctg ctgtggaagc cagcagtaaa tttggtattc
    26161 gggctattat taccgactcg tttaccggat cgacggccag aaatttggcg gcctatcgag
    26221 ggaaaaatcc tgtttatgcc atttgttatc gtgaaagatt gacgcgcgaa ttggctttgt
    26281 cttatggtgt ttttcccaaa tatcaggaag aaaaacgaac caatcaggaa tatttattgt
    26341 tggctttaag ggatttactg gatagaaaac ttttgaccga acaggatttc ttctgttatt
    26401 tgagcggaag ttttggagaa ggtcacggaa ccacttttct ggaaatcaat caaattgaaa
    26461 aagttcttaa agcattgaat aattaccagt taccgagttt ttagccttat ttaaaaattc
    26521 cttttttcta atatccgaaa aacatcaaac agttaaaacg ctaaaatgtt taactgaata
    26581 agtaaatgtg attatcattt cgttaataaa tgattggata gtatttttat agaagtttat
    26641 gcaattacat aacatttaaa aacctatcga aaattaaact ttgttggtgt tttgaagtcc
    26701 attaaatatg taagttttca taaacactta taaaacgaaa agatatttgt atgaaaccca
    26761 catgtattgc atacacaaac acgaatgaat ataatatgtc agacatgttg gttccatcag
    26821 acctttaaaa aataaattat attctgatga aaaaagtatt ttttatttcc tttgtttttt
    26881 tattcttaag ttttgaaatc ttagcttcaa cggttataaa gggaagagta attgatgcca
    26941 ggtctggcac tccattagag tttgtaaatg ttgctttata tcaattaggt tcttctgttc
    27001 cctctgtcgg attaaccacc gatgcaaaag ggaatttcga aatttcctct attagcagtg
    27061 gtaaatatgt tctcaaaatc agtatggtag gctatcaggc ttatgacaaa gaagtagata
    27121 taagaagcaa tattttagaa cttggagtca ttcgtttgga agaagaatcg aaagtattga
    27181 acgaagtggt tgttgtagct caaggttctc aaatgaaatt cgacatcgat aaaaaagttt
    27241 ttagtgttga tcagaacatc gcttcttcag gaggctcagc aacggatgtt cttcagaata
    27301 tcccttcggt agaagttgat aatgaaggaa atgtgtcttt aagaaacgat gcaaatgttg
    27361 aagtttggat caacggtaaa ccttctggac ttacagttga gaatcgtgct caagttttac
    27421 aacaaatgcc tgccgaaagc attgaatcta ttgaaattat tactaatcct tctgcaaaat
    27481 tcaatcctga aggaacagcc ggcattatca accttgtttt gaaaaaaaac agacgtggtg
    27541 gttattatgg tagcctttcg gccggagtaa tgtatccgga tggagcaaaa ttaggtagta
    27601 atggcggaat aaattttaat tatagtagca gcaaaataga tggttatgtc aacatgggtt
    27661 atcgatcgat gaacatgaaa ggcgggggtt ggaacaatcg atacaattat ctcgaaaacg
    27721 atactacttt gctgaaacaa aataattcat cagaaaataa ttttgatggt gtctttatgc
    27781 gtgcaggtct cgactggcat ttgaacgata ataacacttt tggtatctcg ggctttggaa
    27841 tgacacacaa tgaagatgga aacaacaaca ttaattatgg tttatatgat gttgataatt
    27901 ttcttctgaa aaactatagc cgttataatt tggataatgg aaatcatcca agctttcatc
    27961 tgaatgttga ttatcaacac gaatttggaa gaaaagataa taatattatg gccagtattt
    28021 cttattcaaa acataataga aaatctgatg accagtatat tcaaagggat tatctattag
    28081 atcttacctc aagcgacata acacaaacta ccgacaatcg gaatcatcag tgggaattta
    28141 aaatcgacta ctcgaataaa attacggaaa acagcagatt ggaggccggt tggcaaagta
    28201 atcttcagga gcgaaacaat ttaaccagtg ccatagacaa tttgaacaat ggagtggaaa
    28261 tttttagtta ttcagacaat ttcgattata aagaacagat acatgctgcc tatcttacct
    28321 atggtaacaa atttgaccag ttgagtttgc aagccggact tcggggtgaa tatttggaaa
    28381 aaaaaatcaa caatttggag actcaatctt attttgaatt atttcccagt ttttatcttt
    28441 cctattcatt acctgaacaa aacgagttgc aattgagcta tacccgtcga ataaaccgtc
    28501 ccagaggacg catgatcaat cctttcagag attattcgga ttcgaccagc atatcgtatg
    28561 gaaatccaca acttttgccg gaatttgcca gttcgttgga actcaattat ttgaaaacat
    28621 ggaatcaaca ttctctttcg gcgtcggcct attatcgctt tacagataat gttattgaaa
    28681 atgtgcgtta tatgaacgga caaacgatgg aaagtacgta ctttaacatc acaaaatcaa
    28741 aaaatgccgg tcttgagttg gttgcaaaga acaatttgtt taagatattg agtctgactt
    28801 cttctttaaa tttatattac agtcagttgg attcggcttc atttaccagt ccgtacaata
    28861 cgactgttac cattccaaat caggatgatt tttcatggag tggaagattg ctggcaaatt
    28921 taatgctcag taaaaatact tctgcacaaa tcaccggccg ttatttatct ccccgcatca
    28981 tctcacaggg ggaaagagct gccaattatt ctcttgacct gggttttaga caaacatttt
    29041 ttgatagaaa gttaaatctc aacctaatgg ttcgtgattt gttaaattcg agaaaatgga
    29101 aatcaacaac atggggaacc gatttttatc aggtatcgga atcttatttt catggaagaa
    29161 tggtgggcat gactctatct tacaatttcg gcaacatgaa acccaaacaa acagttaaaa
    29221 aaagagcgga atcggaaagt cctttgaata tggaaatgga aaatatagag taagaaggaa
    29281 aggcattgta gttgccaaaa gctttttatt tgagtctgcg ccgaaattcc gcacaaacca
    29341 aagctgccgc tacgcttacg ttgagcgatt cggaagtggt atttccgggt ggatagtttg
    29401 gaataaaaag acgttcggta accaattttt caattgccgg agagatgccg ttcccttcgt
    29461 ttccgagaac aataatgcca tttgaagata ggggagtagt ataaatattt tctcctttca
    29521 gaaaagttcc gtaaataggc agtttatctt tcaatggcaa cagaaaattt tccagatcga
    29581 cttcctgaac ttgtacgcgt gccagcgctc ccatactggc ttgtacaact ttaggagcaa
    29641 aagcatctgc cgaagtttga gaagcaaaaa tatgtttaat gccgaaccag tcggcaattc
    29701 gaataatggt acccatattg ccgggatcct gaatttcatc cagcgccaac gataattctg
    29761 agccgatttc ttccaacgat aaagcgatgt cggtcgcttt gccgaaaacg gccaatatac
    29821 cttgaggatt tttttgattg gaaattttct tgaactcttc aaaatttact tccaatagtt
    29881 cttttgtccg aatgggcgta ttgttttcga gccattcagg tgtaaccact aaaagttcgc
    29941 aatgaaatgt taccgataaa tcgttaatca attttgttcc ttcagcaata aacatattgg
    30001 tttcatctct gaattttttt aggttcaaag aattgataaa cttgatttta tttttcgata
    30061 acatgagttt ttcgtttttt cagcaggata ttttgtctat tcgtcgctga tgtcttcctc
    30121 cttcaaaatc ggtttcaaag aacacatcca cgatttgttt ggcttcattt tcgttgataa
    30181 atcttgccgg caacgaaagt acattggcgt tgttgtgtct gcgtgccagc gatgcgattt
    30241 cgggcatcca gcaaagcgca gcgcgaattc ctttgtgttt gttcaccgtc atgttgatgc
    30301 cgtttcccgt agcacacagg gaaatgccaa aatcaaattc tccgttttct actgccgaag
    30361 ccataggatg ggcgaaatcg ggataatcgg aactttctgc cgtataggcg ccaaaatctt
    30421 ttatttgccc aacatttttc gatttcagat actcaatcag tttgttttta agttcaaaac
    30481 ctgcatgatc gctacaaatg gcaattttaa gttcgttgag tggtttcatg tgattgaatt
    30541 aattttaaag aaattgatga aattccttgc attgatttat ttgtccggaa aaaatttttt
    30601 ctgactgttt gaaaaaattt ggttgctgca ccggcagtgg attgtgtacg attcatgcaa
    30661 atttttccac atcatctttg gtaatcatcg gagaaccgag aataatcaat ctttccacca
    30721 cattgcgcag ttcgcgtata tttcccgtcc acgagcgttg ttgtagagcc tttatggcgt
    30781 ccggttcaaa ttttttggga tgaatacctt cctcagaaca gattaattcc gtgaaatgtt
    30841 ctaccagcaa gggaatatct tcttttcgtt cgtccaaagt tggaacatga attgggataa
    30901 cgttaagccg atgaaacaaa tcttcgcgga aattcccttt ttcgatttct tttttcaaat
    30961 ccttgttggt tgcggcaatt actctgacat tgactttgat acttttgtcg ctccccacac
    31021 gcgaaagttc attttcctgc aaaacccgca aaacttttgt ttgagccgaa agactcatat
    31081 ctccaatttc gtcgagaaag atggttccgt tgtcggcttg ttcaaattta ccgatccttt
    31141 gtcggatggc agaagtgaaa gctccttttt cgtgtccgaa aagttcactt tcaatcagtt
    31201 ccgaaggaat ggccgcacag tttacttcaa caaatggagc attggcccgg ttgcttttat
    31261 catgtattaa tctggctacc aattcttttc ccgttccatt ttcaccggta atcaaaacgc
    31321 gcgcgtcagt gggagctacg cgctcaatca tttggcgaac gttttccaat gcggctgatt
    31381 gaccaatcat ttcatatttt tttgctgtct tctttttcag atttttggtt tccacaacca
    31441 aatctttctt ttccagcgca ttacgtactg taatcaagag acggttcaaa tcgatgggtt
    31501 tttcgataaa gtcaaaagct ccttttttta tacattctac ggctgtttct atatttccat
    31561 gtccggaaat catcacgata ggagcttcaa tgtctttttc tttcagttgc gtaagcagtt
    31621 caataccatc catttcgggc atttttatat cggaaaaaat caaatcaaac atttcgtttt
    31681 gagctgcttc aaggcctttt aaaccgtttt cggcaagtga aacctgatgt ttttcaaatt
    31741 ccagaatatc tttcaaagta ttacggatac ttcgttcgtc atcaataatt aatattttgg
    31801 ccattttcaa attatttctt tcggggtttg tttttcagaa acaaagttaa aaaatatctt
    31861 tgttatttaa ttgcagaagc tgacggactt taaaatttta aaaatttccg gatgctttga
    31921 atgatttcag gaaaaagtcg aaagtaggaa acagactaca gtccaaactc ggagagcgat
    31981 gtgccaacag aaaattgata tacgctgttt ccaacctgat aggtagacat gccaaaacca
    32041 acattgaatt tgtataattt aatgcctccg ccaaaagaaa gacctgcctg cgatttaaaa
    32101 tcttctactt tcatttcttt tgcacgccta aaattatatc ccaatgtcag ataaaaatta
    32161 ttggaaggaa taaattcggc attgaaaata aaatgacgca aagccatgtc aaaaaaatta
    32221 atttttttcg tttcattctg cgtggaagaa gcgtaagata aatcccagtg ctgcaaattg
    32281 tgtaaagtcc ccgtaaaacg cagcggcgca taagccaatt ttttggttat tcccaattgg
    32341 atgttaaatg gaagcggttc gtaatgctgt ccttcttcat cggaataata accttttatt
    32401 tggctgccta tgttgcgcaa taccaatcct gccgaaaaaa aattggttgg gtcgttataa
    32461 ctgatgcccg catctatggc tgccccaaaa ctcgaataac gttcataagc cgagtaaatc
    32521 ggtttaaaag ttcccccaac agaaattttt tcggtaatcg ggtaggcata cattatgtaa
    32581 agtgccatgt ctttggctgt aaattcacca ataatttcgt tttgttccgt tgtttcctga
    32641 aaagtgccgt agtcgacata ctgcatgcca aaagccatgt aatgcttgtt tttgaaattt
    32701 tttccaaaca cggcacttcc atactgaatg tcagccaaat aattggccat gcttaatgaa
    32761 agaaaatcat ttgttttgtc ggtaagcagc gacggattgc aaaaagaaaa gtttatatcg
    32821 ttgtccttca gcgaaacatt ggttcctccc attgcggcaa tacgtgaaga tacaggcaaa
    32881 tccagaaatt gatatacacc tttgcctgcc tgtggataaa ctaaggcaac aaaagttatt
    32941 aggaaccata aaaacaaaac aattcttttt atcattagtt tcttcaaaaa aatttttaaa
    33001 aatattgtag aataaagatt ttttccgttc tatgcaaaat atatcaaagt tttggtaatt
    33061 ttgcaatgca tttaagaact accaatgtta attgaaaaat aacggcaaaa ttaagaaaaa
    33121 ggtacgttcg gagatgactt tttatgaaaa atataatttt gttatcgatt ttgtttaaaa
    33181 gaaaaatttt ttttacccat aaaacatcaa ctattaaaaa aaaattgtac atttgtacca
    33241 tctgaaaaat aaaaaagact ttatcattat ggaagaaaaa aaatcaccaa aagcctcatt
    33301 agaaaataag aaatcattat ttattctgat cggtttggtt atggcattgt catttattta
    33361 tattgctctg gagtggacag ccagagaaat taccatttac gaaggcgttc aaactgatct
    33421 aaaaacggaa gaagagttgg atatcattca aacaacacag gatgaaactc ctccaccacc
    33481 acctcctcct gcacctgaag tgattgaagt actcaatgtg gtggaaaata cggtggaaac
    33541 ccaaaccatt aacatagaaa cagaagatac caaaaataaa gccgttgata ttatagccgc
    33601 gcctgtcgct gcacctattc acgaggaaga tgaacaagtt atttttcagg tggttgaaac
    33661 catgcctcaa tttcctggtg gagaacaagc tttattcaaa tttttaaatg aaaatatcaa
    33721 atacccggtg attgctcagg aaaacggaat tcagggacgt gttatttgcc aatttgttgt
    33781 gaataccgat ggttcaatag ttgacattca ggttgtaaga ggtgttgatc ctagtctgga
    33841 taaggaagcg gttagggtta ttcaatccat gcccaaatgg attccgggta agcaacgagg
    33901 aaaacctgta agagtaagat ttacgcttcc cattaatttt aaattacagt aaattttatt
    33961 tttccttaca ttatactatt tcggttgacc tttcatatcg aaaaaaatat attaaaccgt
    34021 ttggccaatt tatccaaacg gttttttgtt aaagagaata ttaaaattgg aaacaaataa
    34081 ctatcaggaa aaaactattt tggtcggttt agtaacaccg caacaaaaca gggaaaaagc
    34141 aaaagaatat attgaagaat tggctttttt ggcaaaaacc gccggagcag ttcccgaaaa
    34201 aatttttttg caacagttgg atcatcccaa tcccgaaact ttcgtcggtc cgggaaaact
    34261 ggaacaaata aaaaactatt tggcggatca tcccgttgat atggccgtgt ttgatgacga
    34321 actttcagca cgacaaatca gaaatctcga atcggaatta aaaatcaaaa ttttggatcg
    34381 taccaatctg attcttgata tttttgccaa gcgcgcaaaa actgccagtg ccaaaactca
    34441 ggttgaattg gctcagtatc aatatctttt gcctcgccta acacgtatgt ggactcacct
    34501 tgaacgtcaa cagggaggaa ttggcaccag aggacccggc gaaacccaaa ttgaaaccga
    34561 tcgaagaatt attctttcaa aaattgcgca attgaaacaa aacttaaagg aaatcgaccg
    34621 tcagatgagc acacagcgaa aaaatcgcgg aaaattggtg cgggtggcgt tggttggcta
    34681 cactaatgtt ggaaaatcga cgttaatgaa tttgctgagc aagtcgaata tttttgccga
    34741 agataagctt ttcgctacac tcgatacgac tgtcagaaag gtgattatag ataatttgcc
    34801 ttttcttctt accgatacgg tcggttttat cagaaagttg cctcatcatc ttatcgaatc
    34861 tttcaaatcg acactcgatg aagttcgcga agccgatttg ttgattcatg tagtggatgt
    34921 gtctcacccg aattttgaag aacagattca ggttgtcaac caaacgctga aggaaattac
    34981 caatgaagaa aagcccatga tcctggtttt caataaaatt gatgcttaca atcacaaagc
    35041 caaagaccct gatgatttga ctccgaaaaa acctgagaat attactttgg aggaaatcca
    35101 aaaaacttgg atggctaaaa ttcatgaaga ttgtgtgttc atttcagcca caaaaaaaca
    35161 aaatatcgag gaactgaaaa aaattatgta tcagaaaatc aaggctattc atgttcaacg
    35221 ttatccttac aacgattttc tttatcagga ttttgaaatg gaggaaaatg aaacggatga
    35281 ggttgagtaa aataaattaa caattcaatt cttacactta tgaaaaatta tcgacttctt
    35341 actgaaaatg aaattcattt gctcacttct aaaggttgca cggccgaaaa ctgggaaatg
    35401 gtgagagttt cagaaggttt taaaccggat tttatcgctg atgtccattt ttccggaaat
    35461 atttttctcg gtaacttcaa taaaattttt gtgttggcag gaggattgca aagacatgcc
    35521 ggcattcgtc ggtgttgtct gcataattgc acgatagaag atgatgttta tattgatcag
    35581 gtagccaatt atatagccaa ctatcatatt ggcgaaggtt cttacattga aaatgtaaat
    35641 ttgctggtgg tagaaaagga ttcaactttc ggcaacggag taaaagtgcc tgtaatgaat
    35701 gaaggaggag gaagagaaat tccaattttt gattttcttt cggcgccgtt ggcatatgta
    35761 cttactttgt atcgccatcg tccggggtta attcaaaaaa ttcagaagct tattgatgaa
    35821 tacgcatgca aattaaaatc cgataccggc acaattggta aaaatgtcag gattgtgaat
    35881 gccggcagta taaaaaatgt tcgcattggc gattgtgcca atatagaagg tgcttcgtgc
    35941 ctgatcaacg gaagtatcaa cagtaatgct tttgctcccg taaccattgg agccggcgta
    36001 aaatgcgacg attttattat cagttctgga gtttcggtta ccgattccac attgatttcc
    36061 cgttgtttta tcggtcaggg ttgtattttg gggaaacatt attctgtgct cgattcactt
    36121 ttcttttcca actgtcaggg catgcacgga gaagcaaccg ccatttttgc cggtccttat
    36181 acggtatcgc atcacaaatc tactttactc attgcaggaa tgttttcttt tctcaatgca
    36241 ggcagcggtt ccaaccaaag caatcacatg tataaacttg gtcccattca tcagggactg
    36301 gccgaacgcg gagcaaaaac taccagcgat tcttatttgc tttggccggc taaaattggt
    36361 gcttttacgc tggttatggg gcgacacacc aaacattccg atacttccga tttgcctttt
    36421 tcttatctga tagaaaatgc taccgacagt tttttggtgc cggcagtgaa tttgcgaagc
    36481 gtgggaacca ttcgagatgc ccagaaatgg ccaaaaaggg ataaccgaaa agatcccaaa
    36541 aaactcgatc cgattaattt taatctttta agtccctaca ccattcagaa aatgatgaaa
    36601 ggagttgaag tcctgaaaaa cttacagaaa atttcgggag aaacgactga aatttatact
    36661 taccaaaatt gcagcataaa aaattcgtct ttactcaaag gtattgacct gtatcacatc
    36721 ggaatatata aatttttggg taattcgctt atcagcagat tgaaagataa acattttaat
    36781 accattgaag atattcggga aattttgaag cccgatactt ctgtaggtag tggcgaatgg
    36841 attgatttat cgggactgat tgcgcccaaa acggaaattg aacagttgat gaagaaaatc
    36901 gaagatcaac cgattacgct cgaagatatt caaagcgaat ttgaaaaaat gcacgaagcg
    36961 tattacaatt atgaatggac ttgggcaaaa gataagttgg aaatttattg gagaaaacca
    37021 attgctgaag ttacccgtga agatttgata cagatggtgg aactgtggaa aaattctgtt
    37081 gttacactcg atcatcttat ttatgatgat gccaaaaaag aatttagttt gaacgctaaa
    37141 accggatttg gtgtcgatgg tgatgaacag cagaaatctc ttgatttcga atcggttcgt
    37201 ggaagttttg aaaacaatcc ttttgtgctg gaagtcatta accatataaa aataaaaagc
    37261 gaattgggtg atgaactttt agatagattg aaaaaagtta tcaattaaaa aagcagattt
    37321 tttaaaactt attgattatg aacaatttta atttttatag tccaacttat ttcgttttcg
    37381 gaaaaggacg agaaaacgag gcggggaaat acgttaaacg ttttggagga acaaaggtac
    37441 tgcttcattt tggaggaaat tcagccatta aaagcggatt attggacaga gtcaagaaat
    37501 cgctgactca agaaggaatt tcttttgtgg aattaggcgg tgtattgccc aatccgcgta
    37561 gtggtttggt ttacaaagga atagaaattt gtaaaaacga aggtgtagat tttatcctgg
    37621 ctgttggcgg cggaagtgta attgactcgg caaaagctat tgcagccgga gcgttgtatg
    37681 atggtgattt ttgggatttt tataccggga aaaaagttga aaaagctttg ccggtaggaa
    37741 cgattcttac cattgcagca gccggaagcg aaggatccag cagttcagtt attacccacg
    37801 aaaatggctt attaaaacgt tcggcagaag gaaatgcctt gcgccctgta ttttccatcc
    37861 tcaatccgga acttacgttt actttgccag cttatcaaac ggcctgcggc attaccgaca
    37921 tgatggccca cgtgatggaa agatatttta ccaatacccg ggaagtggaa attaccgatc
    37981 gcctttgtga aggaattttg ctgacaatta taaaagaagc tcccgttgca atagccgaac
    38041 caacaaatta tggagcccgt gccaacatta tgtgggcagg aatggtggct cacaacgata
    38101 tttgtggagt aggacgggag caggattggg caactcatca aatggaacac gagttgtcgg
    38161 cactttacga tgtagctcat ggtgcgggtt tggctgttat gtttccggca tggatgaagt
    38221 atgtaatgaa ccacgacatt atgcgctttg ctcaatttgc cgtgcgcgtt tggggctgtg
    38281 aaatggattt ccaacatccc gaaataacag caaaagaagg aatagccaaa tttgaacagt
    38341 ttttaatttc aatcggcatg cctatccgct ttgcccagct gggagctaaa gctgaggata
    38401 ttcctacatt ggtgaaaaac atgagacttg gtaactgtac ggttggaagt tttgtaaaac
    38461 tcaccgaaaa agatgttgcg gaaatttaca aactggccgt ttaaaatttt tatatccggc
    38521 gcaattcagt aattttgtag caatattctc cttttcgata taaggagaat gttgcttttt
    38581 tttattcaat agtaaaatga atagttcttg ttatggaacc caaacattta tattttcctt
    38641 cagccgatat tcatctcgat attttgctcc cggcttcaaa aagcattagc aatcgtgcgc
    38701 taatcataaa tgctttatcg ggttcacgat ctgaaattcg gaatctttcg gactgcgatg
    38761 acacgaaagt tatgctgcgg gcgttgaagc tggatcaatc tatcatagat gttggagctg
    38821 ccggaacagc catgcgtttt ttgactgctt atttggccat gacagaagaa gaatggacgc
    38881 ttaccggaag tgaacgaatg aaacaaagac ctgtgagact gttggtagaa gccctgaatg
    38941 atttgggcgc agaaatagaa tatcttgaga agtcgggttt tccaccttta tcaattaagg
    39001 gaaaaaaatt gacaggtgga acgatacatt tcgatggaag tgtaagcagc caatatattt
    39061 cggctttgat gatgacggcg ccttacatga acgaaggatt gaatataatt ttggaaggtg
    39121 aaattatttc gcttccttat atactgatga ccaagaatat gatgaaagaa tttggtgttg
    39181 aggtcaattt cttgacaaac aggattgaaa ttgctccaca gttttacaaa ccggtagatt
    39241 ttacggttga gtctgattgg tcggcagctt catactggtt tgaaatactg agcattgcag
    39301 gagaaggaga aatttttctg aagggtttgc atcaaaatag ttatcaaggc gacagtaaag
    39361 tggcggaatt atttgaaaat ttaggtgttg ctgttcaata tttttcggac ggagttttat
    39421 taacgcccaa caggaaaaag atagatttct ttaattatga ttttatcaat caacccgatt
    39481 tggctcaaac ttttgcggtt acctgttgtc tgaaaaatat tccgttccat ttaaaagggc
    39541 ttaaaagttt gaaaatcaaa gaaaccgatc gaatctcagc tttaaaaaca gaattggcaa
    39601 aatttggctt tttgcttacc gaacctgccg aaggagagtt gtcatggagt ggagaaaaaa
    39661 aagacgcttg tcattttgaa gagacaggcg aaatttctat taaaacttat gacgatcatc
    39721 gtatggcaat ggcttttgct cccgctgcct tgctgtttcc cattgcgatt gaacatcccg
    39781 aagtggtcag caaatcctac ccaacatttt gggaagatgt agaaaaaatt aaaaagggaa
    39841 aagttaatta atagagcctt aaaaagtttt tttgtcttag tacatgacaa aaaaatttca
    39901 aaacatttat attctttatt ttcagatatt taataacggg actttgcctc aaaccttcac
    39961 ttactttttt tgtcttgaaa caaaaaagta agcaaaaaaa attaatttag ttgaagcagc
    40021 tacgctcaac ataaatcaaa taaatttgat tttgttttcg cttaaacgct gctttcaaga
    40081 ctgtgtccac ttcactcgaa aaacttacgc tcgaaaagct gaaatcgccc aaactcattc
    40141 cgacgaaata cgtcggaact caaacaagga cgatttttaa acgcttttct cacttgtttt
    40201 tcggctcacc ggacaaggtc aaccccaaga gcttgactta tatgtcctgt tttacacata
    40261 aacaaatgtc tcaatttttg tcatgtacta aaaaaggttt taatgacgac cctggctttc
    40321 cggcccatgg tagatttctt ctaagtttga tttccccttt aaattgacta atgagtaatc
    40381 tgggataaaa aaaaaacagg caacttttgg ggaagcctgt tttttgtttc agtaaaaagt
    40441 tcatttaatc agccaacagc atttccatga tggtgcaggc agctttggta acgggagttc
    40501 cgggtccaaa aatagcggct actccggctt tataaaggaa gtcgtagtct tgtggaggaa
    40561 taactccacc cgcaacaacc agaatatctt cacgtcccag cctcttcaat tcttcaataa
    40621 gttgtggaat caaggtttta tgcccggcag caagtgaaga aacacccact acatgaacat
    40681 cgttttccac agcttgcttg gcggcttctg ccggagtttg gaacagcggc cccatatcca
    40741 catcaaaacc acaatctgca tagcccgtag ctacaacttt tgcaccgcgg tcgtgtccat
    40801 cttgccccat tttggcaatc ataatacgtg gacgacggcc ttcctttttg gcaaattctt
    40861 cagtaagctg cgaagcccgc tggaaatttg catcgttttt tgtttctgat gaatacacgt
    40921 ttgaaatagt tcttatgatt gctttataac gacctgccac tttttcgcag gcatcggaaa
    40981 tttcgcccag tgttgctctg acacgtgctg cttctactgc aagctcaagc aagttgccgt
    41041 taccggttct ggcacattct gtaatggcat ccaaagcttt tttcacttct tcgttgtttc
    41101 tggaagctcg gagctgtttc agtctttcaa tctgttgcat gcgtactgcc gtattgtcga
    41161 tttcgagaat ttcgatgggt tcttcttttt ccagcgtata tcggttaact ccaacaatta
    41221 tttgtttccc ggaatcgatg cgtgcttgtg tacgggcagc agcttcttca attttcattt
    41281 tcgggatacc ggtttcaatg gctttggcca ttcctcccag attttcgatt tcctgtatca
    41341 agtcccatgc tttttcggcc aattctttgg tcaaggattc tacataataa gaacctgccc
    41401 aggggtcaat ttgtctgcaa atattggttt cttcctgaag ataaatctgc gtgttgcggg
    41461 caatacgtgc cgaaaagtcg gtaggcaagg caatagcttc atccaaagcg ttggtatgca
    41521 gcgattgtgt atgacccagc gctgctgcca tggcttcgat acaggttctt cctacattgt
    41581 tgaaagggtc ctgttctgta agtgaccatc ccgaagtttg ggagtgcgta cgaagtgcca
    41641 tagactttgg atttttagga tgaaactgtt tcacgatttt agcccagaga agtctggccg
    41701 cacgcatttt ggcaatttcc ataaaatgat tcattccgat accccagaag aaagatagac
    41761 gaggtgcaaa agcatcgatg tcgagccctg cattgatacc ggtacgcaaa tattccaaac
    41821 catcggcaag tgtataggcc agttccaaat cggcagtagc acctgcttcc tgcatgtggt
    41881 aaccggaaat ggagatggaa ttgaacttgg gcatattttg cgaagtatat ttgaaaatat
    41941 cggcaataat tctcatggaa aattgtggcg gataaatata ggtgtttcgc accataaact
    42001 ccttcaaaat atcgttctgg atggttccgg aaagttcttc gagtttagct ccctgttcga
    42061 gccctgccac aatatagaaa gcaagaatgg gcagtacggc gccgttcatg gtcatggaaa
    42121 cggacatttt attcaaggga attccgtcaa agagaatttt catatcctct acggaacaaa
    42181 tggatacgcc tgctttcccc acatcgccca caactctttc atggtcggca tcgtaaccgc
    42241 gatgcgttgg caagtcgaat gctacggaaa gccctttttg acctgcagcc aaattacgac
    42301 gataaaaagc attggactct tcagcagtag aaaaacctgc atattggcga atagtccacg
    42361 gacgcgttac atacatgctg ctgtaaggac ctctcaaaaa aggaggaatt ccggctgcat
    42421 aatgtaaatg ttccattcct ttccagtcgt ttttctcata aaaaggctta acgggaattt
    42481 gttcgggcgt tagccaattt tcttcagtct gaagcatggc agctccactt gaagtaatgt
    42541 tgctgacatc aatgttgtta aaattcggtt tcatattttt tttgtttttt gaatcagact
    42601 ttccgacgac gaaaattcaa tggtcagtcg aaaatactgt atttcaatgt ttactttttt
    42661 ctaaaataaa atcattaatc gataataaaa aatctaatag gacaagttat tgtaattttt
    42721 cagttttgga cgttgataat ttcatttttc acacgatagg gaataatata aaaacggaaa
    42781 catcccacac cgcatgtgaa acaatcaagg ttaccaaatt tttattgtat ttgtaaagca
    42841 atccccaaaa tgcaccgcaa accatggcag acataatcag catgaagtta aacgaccaaa
    42901 tgtgtaccaa agtataaatc agcgttgtaa ctatcaatgc catccatgaa ccaaattttg
    42961 gttccagcat tcgctgaacg tatcctctcc aaaaaatttc ctctgccggg ccaatcaaaa
    43021 gaatgagcaa taaactcaac aacaaaggat tttctccttc tttcatcatg taaatctgcc
    43081 caacctgatc ttttgcaaaa tcgaacagca attttgaaaa gaaatcgccc aagaaaaaca
    43141 ccacatataa aacaacagcc gatcccagac caatgagtac atctttcagc gaaagcgaaa
    43201 attgtttttt gaaatctttt ccgagaaaag cactcatcaa agtcagcgtt atgccggcag
    43261 ccgtcatggt cagccagaag ttgacatatt ctttggtcca gggagaaaac atgacaaacc
    43321 aaaaaatgat agcaatcagt atgggaataa taattctttt cataaccatg cagcaataaa
    43381 atttgaaata gagaacaaca agctgaatac aagtaccaac tgtgcagttt gtgcatccaa
    43441 atcaaaaatc ttttcgggtt tttccacttc tgcggtcatc atttttttga tgtttttcac
    43501 agccaaaggc aacgaaagca acatcagcaa agcaaccgga tgaatcattt tgaaaataat
    43561 catcaacacc acacccacat aagctccaat actcaaaaca acatactgta tttttgcacc
    43621 tttgataccc aacagcatgg cttgtgtttt gatgttggct tttccgtcgt cgcgaatgtc
    43681 tcttgtgttg ttggcatgta aaatgttgac aaccaagaag gcaacaggta tattcaacac
    43741 cactgcaaac cacatgagtt gtccggtcat gacgtaagct gtacccaacc cgatcagcgg
    43801 cccgtagatg ataaatatag tcaaatcacc tagcgcagcg aatttcaatt tgtaatagaa
    43861 ataagttgcc attgcgccaa tcgctccgat ccacaacaaa tcgatgcccg ttcggtaaca
    43921 aagaaaaagt cccagcacaa cacctaccgc cagcaaaaca atgccaaaat gcaaaatcgt
    43981 tttgggttga aaaacctgat cgaccaacat tcggcttgaa ccaaacgtct ctttacggtc
    44041 cacatggtgt tgatagtcga aataatcgcc aatcagattt ccgctggctt ggaaaatggc
    44101 ggcacccaaa agtgccaaca ccccaaacca ccagttaacc tcgcttccaa gctcattttt
    44161 cagaaaaaag acatacgaaa tggtcaacaa tgcaggcatg gttgaagcag gaaaagacca
    44221 gggcctggta gccagcaatc cgtttttaat tttttctttc attttgaaga tgttggatag
    44281 aaaaaatttt ttgactggag aatttatttt ttataaatga aaatgatata caaaaacttt
    44341 acagacccag ttttttgttg aaatccttca aggtttccaa cacattggat tttatgctga
    44401 taaagttctc aatcccttcc tttttcaaat cttccataca agcgggagcg cctgccacaa
    44461 caaaaatcgt tttgtccttc aacaatttgt aagcttcggg ggctaatgtg gcatattctt
    44521 cgtcgctgga gcaaagcaca acaatttcgg cttttgcatc caacgctgct tttacgcctt
    44581 cttccacaga agagaatccg agattgtcga taatttcgta tccggcacat ccaaagaaat
    44641 tgcccgaaaa ttgagctctt gccgagcgca tggctaaatt tccaacggtc aacataaaca
    44701 cttttggacg cttggacgtt ttttcgaccg ctaagcgtaa gttttcaaat tccacagcac
    44761 cacgcaacgg aagaagcttt tctgttcctt cttcatgcga acagcaacca cactgttgca
    44821 attccttgtc gattttttcg gcagcagttt ccaataaatt tggatattga ttggtgccta
    44881 ccaaaatttc tttccggttc gagatagctt ttaaacgttc attggctgta gccttgatgg
    44941 tctgttgaac ctttccgttt tgaacggcag caaggaatcc gccttcgttt tccacattga
    45001 ggaaaagttt ccaagcctga ttggctattt cggcagttaa agtttcgata taataggaac
    45061 cggctgccgg atcaacaact tcactgaaat gagcttcttc tttgaggagt aattgctgat
    45121 tgcgggcaat tctttccgaa aattcgtcgg aaggtttgaa agcagtgtca taaccgagca
    45181 ccgacaaaga atcaacacct ccaatagttg cactcatggc ttcggtttgt gtacgaagca
    45241 tgttgacata agcatcgtaa atggtttgat tgaaaatggt cgtttcggcg tggatacgca
    45301 tttttgctgc acatttgcaa attccattta ctgtattggg acagtcggga cgaggacagg
    45361 gaggttgata agcatcaaca attaatgccc acaacatgcg tgcagcacgg aattttgcaa
    45421 tttccataaa atagttgctg ccgataccaa aattgaattt tatttttttg gcggcaaccg
    45481 ctttatccac tcctgcgtct atcaacatgg aaaggtattc gtttccccat gccaaagcgt
    45541 aaccgagttc ctgggtaatg gtagaacctg cgttggagag agtggtggca ttcacgccaa
    45601 ttacacgata aaaaggcaat ctgcttattg cctgaacaac agctttagcc ttttcggcaa
    45661 tctcttcttt cgtcaatttt ttgcctgcca gcaacatgcg attaatcggg tcgaaattga
    45721 tagaaccctg caatttcttg acctcataat tttgagcaat gaaatactcg gagagaatat
    45781 cggcaagttc ggcagctttg ctgatgcaaa tcgtgaaatt caactccaca cagtcagctg
    45841 caatatcctt gagcaaagtg gcaacaaatt ctttagatag atctgctttt ttcagctgaa
    45901 atccaatgga gttcacccct cgatttagaa tatacagggc tttagcattg gcttcctgtg
    45961 cgttttcaac ttttatgtcc tgccgtacaa accattcatt atcgcttttt gtccccctca
    46021 cataagggaa agctccgggc aacaattcgg tgattttcag atcatccaca tcttcctcgc
    46081 ggtaaaaggg tctcacattg aaaccttcat tggttctcca gatcattttt ttctcaaaat
    46141 cggctccctt gaggtctgca atgattttat ccagccattc ctgcgtggag acaggaggaa
    46201 aatcagctaa taaatttaat tttttttctg ccataatatc aaatttttat cgaaatttct
    46261 ttgagctgca aaattactac tttaaatcga ttctttaaga ttttttggga ttaaataaag
    46321 aaaaaaagtg gtaattagtt ttgaatctaa gctgaattta tataaacaat gacgagaaat
    46381 ttttcctaag aaaaaaatta aatacttggc atacaattat ctgacaaaaa atgaactaaa
    46441 aaatatttca actgccttta ttcaattcaa tttttttaat tacatttgtt tttcaatgca
    46501 aacgtttgcg tcaatttaaa aaatatgaat catgcaaaaa ctaattattt ttattttttc
    46561 ggttttattt gcctttcctt tatacgccaa acgcgtaatg acagggattg aagtactcag
    46621 agcagataat ttcaaattgt tggaaggcaa aagagtaggt ttgattacca atccgacagg
    46681 tgttgatgga aatttaaaat ctaccatcga tattttatat gaagcacgca acgtaaaact
    46741 ggtagccttg tacgggcctg aacacggcgt aagaggtgat gtttatgcag gcgataaagt
    46801 tgtgaatgcc acagatgaga aaacaggttt acctgtgtat tcgctttatg gaactacacg
    46861 caaaccaact tctgaaatgt taaaaggaat tgatgtgctg gtttatgaca ttcaagatgt
    46921 gggttgtcgt tcctatacct atatcagcac catgggttta gctatggaag ctgctgccga
    46981 aaacgatata gaatttattg tgctcgatcg tcccaatcca ctcggtggag aaaaaattga
    47041 aggcaacctg acggaagaac cttttatttc ttttgtcagt cagtttaaaa ttccttatat
    47101 ttacggtcaa acttgtggag aactggccct aatgttaaat gaagaaaacg cactgaaaaa
    47161 gaaatgcaaa ctgacggttg tcaaaatgaa gaactggaaa agaagtatga aatgggaaga
    47221 caccggattg gaatgggtca ttgcttcacc tcacattccg cataaacatt cagcttacta
    47281 ctatccggtt accggaattt tgggtgagct gggttacgta tcggtaggcg ttggctacac
    47341 ccttccattt caagttgttg cagcgccctg gataaaagca caggaatttg ccgatgccat
    47401 gaatcgttta catttgccgg gcttcatttt tcgacccatc tttttcactc cattttatgg
    47461 aactttaaaa ggagaaaatt gtcagggagt gcagattcac attgtcgatt tcaaaaaagc
    47521 ccatctgtct ttggttcaat tttatattat gcaggagttg gcacgtttgt atccggataa
    47581 gaaagtattc gacaatgcca acaaagaaag attttccatg tttgataagg taagcggaac
    47641 cgatcaaatg agaaaactgt ttcaagttaa caattcggta tccgacattc agcactattg
    47701 ggacaaagat attcagtcct attatcaact ttcaaaaaaa tattatttgt atcactgatc
    47761 aaatgaaatg ataaactcct ttttatccga aattaaaagt tttatccccg aaaaacgaat
    47821 ttacaccgac gaattacgtt tactcgcatg gggcacggat gccggcttct atcgtttgat
    47881 accgaaagca gttgttcaat ctgccagcga aatggaagtc agtttgcttt tacgcgctgc
    47941 aaccaaacat catcttcccg taacttttcg tgctgccggt accagccttt cgggacaggc
    48001 aatttccgat tcggtgctga ttcttgccgg tagcaactgg gaaagatacg aacttgctcc
    48061 cgatgcttct ttcatccgcc tgcaaccggg aattgtggga gaacgcgtta atcagatttt
    48121 aaaacctttt agaaaaaaat ttccacccga ccctgcttca attaaatcgg ccatggtggg
    48181 aggaataatc gttaacaatg cttcgggaat gagttgcgga atacgcgaaa acagttatag
    48241 aatgttgatt tcgacaagaa tagttcttcc cgatggcagt gtgttggata cgggcgacga
    48301 agaaagtaaa gcggtttttg ctaaaactca tcccgaattt cttcgcaaaa tagaagattt
    48361 gcgagacgaa atacgcaacg acgtaacatt aaaacaactg attgaaaaga aataccggat
    48421 aaagaatgtt accggtttat ccatcaattc ttttatcgat tttgacgacc cattcgatat
    48481 tattacacat ttaatggtcg gttcggaagg aacactcgct tttctttcgg aagcagtaat
    48541 gaaaacaact accgactatc cgtacaaggc aagtgctatg gcatatttcg acaacatgca
    48601 tttagcttgt gaaaccgtaa aaaatttaaa atcttttcct gtcagtgctg tcgaattttt
    48661 cgaccgtaaa gccattcgtt cagtagaaga caaatttgaa acgatatctg agctaaaaac
    48721 tttgccggat aatagtgcag ctttacttat aaaaattgaa gcatcagatg gaaatgaatt
    48781 gaatgaaaga attcgacaag tggaaaaagt cctgaatgat gctcatacgc ttctacctgt
    48841 gagctttacc gacaaagaat ccgaatacaa aaaatattgg gacatgcgtt caggaatttt
    48901 tccttcggtg ggtagcaccc ggccagtagg aacaacttgc ctgatagaag atatagcttt
    48961 ctgcatagaa gatttagcgg aagcaacaga cgatttgcac gaaattttta taaaaaacca
    49021 ctatgaagat gccgtcattt acggacatgc actcgatgga aatcttcatt tcattatcag
    49081 tcagcgtttt gatacggaag aatatattca tcagtatgaa aaaatgatga atgaagtaat
    49141 tgaaatggtg gtaaaaaaat atcacggttc gctcaaagcc gaacacggaa cgggcagaaa
    49201 tatggctcca tttgtaaaaa aggaatgggg agaaaaggct ttcgacatta tgctgcaagt
    49261 gaaacaactt ttcgatccgg aaggaatcct gaacccggga gtaatattca atgacaatcc
    49321 gcgttgtcat ttggaaaata ttaaacccat gccggctgtg catccgctta tcgataaatg
    49381 tatcgaatgc ggcttttgcg aagtaaattg tgtaagtgcc ggtttcacca tgtcttcgcg
    49441 gcaacgaatt gttgtgcaac gcgaaattaa aagattgaaa acttctgatg aaaattcccg
    49501 acgcttgaag gaattggaga aaagttatcg ctttatggga gaacaaagtt gtgccggcga
    49561 tgggctgtgt gcaacatctt gtccggtttc gatcaatgtt ggcgactata tccatgtgct
    49621 tcgcaatgag aacatgaaaa acaaaacaat ggctcaattt ttgggacgct ggactgccaa
    49681 acatttcaat tgggtggctt caggtttgaa atcggtactc tatattgctt ccgctgcaca
    49741 acacatattg ggtaacaaag caatgggtgc aattactcgt ggaatacaca aatggagtgg
    49801 cggtgttttc cctttgtgga caccggccat gcccaaacga agaaataaaa tagaagtatc
    49861 aacttcaacc tcgactgact catcattgaa agtagtttac tttccaagtt gtttgaatca
    49921 gatgatgggc gtaagtaaaa atgacccaga gctgacgcca cttgtagaaa aaaccgtatc
    49981 tttgctccat aaagcaggct accaagttgt ttttccaaaa aacatggaaa aaatgtgctg
    50041 tggaagcatt tgggaaagta agggattgct ttcggtggac gaacagaaat cgtcagaatt
    50101 ggaaaaagca ctacgtgaag cttccgaaaa cggaaaaatt ccgattcttt gtgatcaaag
    50161 tccttgtctt tatcacatgc gcaagtcgat gaaacaactc tcactatatg aaccggttga
    50221 atttatacac gattttctac tggataaatt ggatttttac cctatcgatg agcccattac
    50281 tatccatgcc acttgctcca ccatcaaaat gggattgcag ccaaagcttg tagaaatagc
    50341 caaactttgc agtactcagg ttttggtacc tgaagaagtt ggatgttgcg gctttgccgg
    50401 cgacaaagga tttaccatgc cggaactgaa tgctcatgcg ttgagaaaac ttcgcccaca
    50461 aatagaaaaa gcccatgtta aagtgggtta cagcaacagc cgtacctgcg aaattggttt
    50521 aaataccaac accggaattc cataccagtc gatagtatat ttggtggata aatgcacaaa
    50581 aaataaaata aacagatctt aaaaaatttc acgaataaaa tcatgagaaa atttcgtttg
    50641 tcagtagttt tattattctt tgcattctcg tttttgcagg ctcagccgaa gcgggaaatg
    50701 agggcagtgt ggatagcaac agttgccaac atcgattggc ccagctccaa tcggttgtta
    50761 cccacccagc agcgaatcga aattgtgaac atgctcgatc ggctgactga aaacaacatt
    50821 aacgccatag ttttgcaaat tcgtccgatg gctgacgctt tttatccttc taatctggag
    50881 ccctggtccc attggcttac tggccgacag ggcgtgcggc cttcaccttt ttacgatccg
    50941 cttcagttta ttatagaaga agctcacaaa cgctgcatgg aagttcatgt ctggctaaat
    51001 ccctatcggg taacaacaac cggtgattcc atgatgctat cgaaagagca tttgttttac
    51061 aaacaaccgg agctttttgt tcgttatggc ggaaaatatt atttcaatcc cggactggat
    51121 gaaacacgga attttttaaa caaagtggtg gaagatattg tagaacatta tgatgtagat
    51181 ggggtacatt ttgatgatta tttttatcct tatccggtga aaggtgaaga ttttccggat
    51241 gatgaaactt tcatgaaatt tccaagaggt tttgctccca atcagaaagc cgactggaga
    51301 cgtaacaatg taaatatggc tattgccgaa gtacaacaaa ccattaaaaa cctcaaacct
    51361 tgggttgaat ttggcgtaag tccttttggc gtatggagaa acgacaatgt cgatccacgt
    51421 ggctctgcaa ctcgcgctgg cattcaaaat tacgacgatt tgtatgccga tatattgaaa
    51481 tggctggccg aaggaacgat agattatgtt acaccgcaat tgtattggga aatcggtaaa
    51541 aaagttgcta actacgaagt tttgttgaaa tggtggatag aaaattctta cggaaaaaat
    51601 ctgtatatcg gattaattcc gtcctctttg ggcgataaaa aaaatccggc atggacaaca
    51661 ggaaatgaaa ttattcgaca aatcaaaatg aatagaaatt cctcacaagt ggacggagcc
    51721 gttttcttca gtgcaaaaac ctttctgcaa aacagacagg ggttaaatga cagcttgcaa
    51781 tcgaactatt atcgttatcc ggcactatgt ccaataaaca gaaatatttt aggcgaacct
    51841 tctgcgcagc cgcaaaattt gcgggttttg agagatggca aggatgcttt tctgttgtgg
    51901 gatgaagtga atgaagaagg aggctgccaa actgcctatt atgtggtgta tgcttttaag
    51961 ggaaaagaag ttggcgactt gaatgatccg gtcaacatta tagctcgaac aaccgaaaat
    52021 tgcatcgatt tgcgaatcct accccacaaa tttaagggaa attatacatt tgtggttact
    52081 gctgtcaatc gtttcaggca agaaagtatt ccgacgcaag gaatcacacg taagttgtaa
    52141 tttctgattt gcgaaaaaat aattttaaag aagaggactg aaaagacgcg aaacggaatc
    52201 catcagtttt tttgtttttt ttcggttgtt ccactgttcc agcgtaattt cctcacagta
    52261 atgtatatct tccaagaaca gctctttaag tgtaagggct gttttttctt catatacaaa
    52321 agcattcgct tcgaaatttt ggtaaaaact tcgataatca atgttacaag agccaacaat
    52381 ggaaacaaaa tcatccatca ccacagtttt tgcatgtaaa aaaccttttc gataataaaa
    52441 aacacgaact ccggcctgca gcacttttcc cagataactg gaagtactgg caatgacaat
    52501 tggcgaatcg gattgtgtcg gaatcattat cctcacatct atgccactca aggcagccaa
    52561 ctgcaaacag ccttctatca gttccgtcgg catgtaatag ggtgactgaa tataaatata
    52621 tttttttgcc gacataatgg ctttggctat gccttgcata atggcttccc attcggtgtc
    52681 ggggccactc gaaacaattt gtaccagatt gttgtcaaac tgcgctggta ccggataata
    52741 ctttttgtcg gtaatcagtt ttttttccac aaaataccag tcgctcagaa aaagatgctg
    52801 taatccatga acagcagaac cttgaatttt gagaaaagta tcgcgccaat aacccagacg
    52861 gttgccatac acgtatcggt cggcaatatt cattcctccg gtaaaagcta cttttccgtc
    52921 aacaacaagt aatttgcgat gattgcggta attgatccgg ctgcgcgaaa ttcggaatcg
    52981 aagcgggaaa aagggacgaa cgtaaattcc tgcttttgtc aaagttttta aaaattttcg
    53041 agaaagatta aaacttccaa taaaatcgta aataattctg attctgactc cttcgtgcga
    53101 tttttttatc aataaatctt tcagccgatt tgtaatgctg tcgtcgctga ttatgtaaaa
    53161 ctccaaatga atatgctccc tggcctgttc cacagcttga aaaatggatt caaaagcttc
    53221 tttcccgtca gaaaaaactt ctattttatt gtaggtataa cctactgctt cgctattacg
    53281 gtacaacagt ttgattaaat tcatttgatg ggcatccagc aaatccgaat tgattttact
    53341 gaaatcgaat gaagaaatgt cgcggtgatc gagactgtga atacttttac gtgaaataat
    53401 tttctgtttg cgcaaatcct gtccgaaaat caggtaaaga atcaatccaa taaccggcag
    53461 aaaaagaata atcagcatcc acgacaagga tttcgacgga ttcctgtttt cgagcaaaag
    53521 cacagaaatt gttgtcagta tggtatagac atacaaggct ataaaaaaaa tcgaaataat
    53581 ttccgacatt tacttaattg tttttttcgg gttataaaaa tgaagccatt cgtgtccgtc
    53641 aaaatttttt ctgctcgccg gttgacaagt cataactggt gcggcaattc cgttgccaat
    53701 ttcgattgga gcaacttcta cattggcttc atcccacaaa ttttcggcta taaaactatt
    53761 cagcaattgc gctccacctt ccaccagaac agaatgaata ttccgatcaa aaatttcctt
    53821 taaaattccg cgtaatcctt cttttttaaa atcaatttga atatattcca cattgttaag
    53881 ttttgcaggt tgcctttcgg taaaaactaa cgtaggaacc aaaccatcga gcaaatgata
    53941 attttccgga atccttccct gtctgtcgag cgtaattctg atgggtgttt ttcctatcca
    54001 attcctcacg gtcaaacttg ggttgtcgag gagcacagta ttggtgctca ccataataga
    54061 ctgattttca gaacgcattt tgtgggtcag ttgtttggtg atttcgtttg aaattatcaa
    54121 aggcggttcg ctgacattat ttctaatgcg atcaataaat ccatcaatgg tttgtgccca
    54181 ttttaaaaca atgaagggtc gtttttgttc ctgaaaggta aaaaaacgtt tgtttagttc
    54241 tctacatttt tcttcaagta ctcccacata cgtttcaatg ccggcatctt tcagtatttg
    54301 gattcctttt cccgaaactt tgggattggg gtcaatcgtg ccgataacaa cgcggggaat
    54361 acctttttca acaattaaac ttgcacatgg aggcgtattg ccatagtgcg aacagggttc
    54421 caaattaacg tacagcgtag catctttcag taattcgggt tgttttacgg aaaaaatagc
    54481 attgcgctcg gcgtgtgctt gcccaaaatg ttggtgaaat ccctctccaa taatggtatc
    54541 gttataaacc actaccgcac caacaagcgg atttggagca gtgtatccat ccccaagcga
    54601 agccagttgc aggcatcgtt gcatatattt gatttcaata tccatgtcgg ttctttataa
    54661 aatcagttct aaacaaaaga aattgtaaca aaaaaagcta acgagaaatt ttggatattc
    54721 tttatctttg tatcatggaa aatattcttc aatacataca ttccgagctg aaaggaatct
    54781 atcctgaaag tgaaattcgg tttatcggct atttgttgct cgaaaaattg accggttttt
    54841 cacgaacaga aattttgcta aacaaaaata cacaaatttc ttgtgaagca gccaaacaat
    54901 tggatggttt tttaaaaaaa ctaaaaaatc atgagccgct acagtatgtt ttaggagcta
    54961 ccgaattctg cggactgagt ttcaacgtga atccgtcggt actaattcca cgacccgaaa
    55021 cagaagaact ggtggaatgg atatgttctg aaaacgaccg aagcaaaccg ttgaaaatgc
    55081 tggatattgg caccggaagc ggatgtatag ctatcagtct taaaaaaaac tttccattgg
    55141 ccgaagtgga cgcttttgat gtttcgccag aagctttgaa agtggcaaaa gaaaatgcca
    55201 aactgaatcg tgtaaatgtc aatttcagag aaatagatat ttttaatcct cctgtattca
    55261 gtaaaaaatg ggatttgatt gtcagcaatc cgccttatgt gcccgaaaac gaaaaaagtg
    55321 aaatgcaacg caatgttacc gattatgagc cttccttggc attgtttgtg cctgacaatc
    55381 atcctctgaa attttatgaa acaattacca actttgctcg attaaacttg gcaaaaggcg
    55441 gaaaactata ttttgaaatt cactatcgaa gtggaaagaa catgctggat ttgctttccc
    55501 aaaacggatt cagagaagtt caattgaaaa aggacctttc cggaaaagac cgaatgataa
    55561 gtggaatttt ttagaatttg tttgaaattt ccgatgattt ttctgctgac aaaagccgaa
    55621 aaaaacatac agcaaatttt taataaacca atccgttgga aagaacccgg ttcaatcatg
    55681 aaacaactct actttaaagg gtctttaaat gatatttgaa tgctttttaa atgttgtttt
    55741 ttgtggaaag catatttatt ttttgtcgaa atgtaatttt ttctcaacaa attcaataat
    55801 ataggagatt gtttcttcga ttcccaatgc ggacgaattg atgcacaaat gataggaagc
    55861 agccgcaccc cattgtttat cggtataata attgtaataa tcggctcttt tcttatccat
    55921 tttttttatg tagtccatcg ctttttcttc tgtcattttc tgattgcggc atacgcgggc
    55981 aattctgtca ggcaaatcgg cagtaataaa tatatttact gctcttggat gatcctttag
    56041 cacataatcc gcacaccgac ctacaaaaac gcatgattgc ttttcggcca aactgcgaat
    56101 cacatcgctt tgaatcttaa acagcatttc actgctcgga taattatcat taaaataata
    56161 ttcatcaatc ataatggaat ttcggagtcc aaacattcct ctgaaaaatc cctgaccttt
    56221 tttttcgtcc agctgttcca gaaattcttt tgccagccca ctttcctgcg aggcgatctg
    56281 aatcagttcc ttgtcataaa acgatattcc caattcgcgg gcaattcctt gcccgatcaa
    56341 atgaccgccg ctacccaatt ggcgtccaat gttgataaca aatctttgat tcataaatat
    56401 agttaataac ttccactctt tttcgacaaa tttttacttc atttattcgg tttttaaagc
    56461 aaaacaaaat attttttccc cttttgctgt aaaaaaatat tttattggtc ttcattcttt
    56521 aaagaaacaa ggatgaggat gaaaaatttt aacataaaac aaaagtacaa aatttggatt
    56581 aaaattcaaa tgcgatcgaa ataaaaaact cctgtcagat gaaactttgt tcttaatttt
    56641 gcttgttgcc tattataaat ttcttcaaga aaaatgtcga gactgacaaa gaaaatatac
    56701 aataggatga attctggcgt ttgctttttt tactttttat caaaatgttt atcggtcaat
    56761 agtttattgt attgatatga ttttttattt tatcaacact tctaatggag aacgataact
    56821 ttgtgcaaaa agatttactg catttatcca gtcgttgtcc gttggaaaac cccatttgtt
    56881 ccaacctcca ccaaaaaaat agacaaaagt atttcccggt ggatattcgg aaataagcag
    56941 agcgtgattg tttttaatca ttgctttcgt aatgggagcc ggtacaaaaa ttccaacgta
    57001 atttctgcct gaaggaactt tggcatccga aacggcatct tcacaataag ctgctgttcc
    57061 ttcttcttgg ttcatagaga gtttaccggt attttcatgg agaaatattc cggctgccaa
    57121 tggtaaggac tgttgatagc cgttaaaggt aacttcggct ttatttagca atgttcccgc
    57181 actgacggta atagttaatt gttctttgta gaacttgtct ccaaccttta tcgaatcgta
    57241 aaataaagag aatttcgatt gaagcggccc aacataatga actttgtaac tgttgaaata
    57301 attcccaatc caaagagaat cgttccaaaa aggagcaatg ccgccagccc ccaatgtgtg
    57361 cccaactttg tagcagtcaa gtcctttccc atgatcaatg tggtaggatt gtcctttttg
    57421 aagttcgtta aagtaaaatg tatcaacaat gagttctttg gtacgtttta gccataaatc
    57481 tactccgttg cttggatttt ctccggccaa agccggcccg tacattcgat aggctgccaa
    57541 gtcgttttcc catgcaaaat catcttttct ctcaggtaca aagcgagcaa aagtctttgg
    57601 ttctaccggc gaaggatttc cttttttcaa gtgataggca acttttttgt ttgcagggac
    57661 agcagcctga aaaataaaag tttcaagatt tccattctca tttttaacca attggtaacc
    57721 aatttcctga ttgtgattgt tctttaaaat ccatttgtcg ctttcttcaa acagttgcaa
    57781 aaattctttg tcatttattt gaattatttc gtcgtttcga tcaaatgaac ccgtattgga
    57841 aatttcaatt gtttttattg aagacgaaca accggccatg agtacagtag ccagatagag
    57901 gaatattttt ttcatgatct aaaagaattg gagtcaatta tttttcctgt acggaaattt
    57961 aattgactcg ttgaaaaatc tttgtttaat taagaaggtt gttttcccaa ataagccaaa
    58021 attccgccat ctacatacag aatatgccca tttacaaaat cggaagcgct ggaagccaga
    58081 aaaacagcag gtccctgcaa atcttcagga gttccccaac gtccggcagg tgtgcgggta
    58141 acaataaatg tattgaaagg atggccttct tcgcgcaaag gagcagtttg aggggttgag
    58201 atataacccg gcccgattcc gtttacctgg atattgaaag ctccccattc acaagccaaa
    58261 ttttttgtaa gcattttcaa tccacctttg gcagcagcat aagccgaaac ggtttcacga
    58321 cccaattcgc tcatcatgga acagatattg ataatttttc cgccgccttt ggctatcata
    58381 cccggagcaa cggcttttga aacaataaaa ggaccgttca aatcaatatc caccacttgt
    58441 ttaaaatctt tggcgctcat ttccaatgct ggaattcgct taatgatacc ggcattgttt
    58501 accagaatat ctatgattcc gacttctttg gtgattttgg caatggcttc tgttactgct
    58561 tcttcgtccg taacatcaaa aacgtaacca tgtgcatcaa tgccatcggc tttgtaactg
    58621 gccaatccca tgtcaacttt ttcctgattg atatcgttga aaactattac tgctcctgca
    58681 gcatgaaaag cttttgcaat ggcatatcca atgccgtagg atgctcctgt aatcagagca
    58741 atttttcctt ttaaatcgaa ttgattcatg tcttttaatt gttattttga ttcgttgata
    58801 atttattgat tttcatgtaa ataattaaat attaatcgaa tttaaatatt atttcagatc
    58861 ggtttttttg taaaaatcct gatcatcata atccaaattt tcacctgcca ttccccaaat
    58921 aaaagtataa ttgcttgtag cagcagccga atggatggac cattgtggag aaataacagc
    58981 ctgatcgttt ttcatccaga tatggcgggt ttcctcgggt tcgcccataa aatgacaaac
    59041 agcttgatca tcaggcaact caaaataaaa ataggcttcc attctacgac tgtgagtatg
    59101 ggcaggcatg gtattccaaa cacttcccgg ctcaagttcg gtcattccca tttgcagttg
    59161 gcaggtttcc agaacctgag aaaccatcat tctgttgata aaccgatgat tggagccttc
    59221 taaactcccc atttccaaaa ttaccgcatt ctcttttgtg atttttctgg tggggcaggc
    59281 tttatgagct gtagctgaat taaagtaaaa ttttgcggga ttggaaccat caacgctttc
    59341 aaaagtaact tttttgttcc ctctgccaat gtacaaagct tccttataat ccaattcata
    59401 atttgtcccg tcaacgttga tacatccttt accaccaaca ttgaaaatgc caatttctct
    59461 ccgttcgagg aaaaaatcgg attttaaagg atcaatcgat tccaatgtaa gtacttcggc
    59521 aacaggcatg gcaccgccca ccaacatcct gtcgaacagg gagtaaacca tattgacctc
    59581 atttatggtg aaaattcttt caaccaaaaa ttctttcctt agtcgtgagg tgtcgtaatt
    59641 ttttgcatct tgtggatgag aagcataccg aatttcataa ttagttttca taattgattt
    59701 ttttgaatgt tatttgtaat ttttatgtgt gcatgcacat aaaacattgt atgatatacg
    59761 ttttaatttt tgattttcat ttttagttca acgaaatttt gttgattgag aaaatttcat
    59821 tatagtttcc cgataaacca aattgtagtg taactctcag taaactaaac tattatgact
    59881 aaaaaataaa aagtaaaata attttcgttt gaataaacaa agttacaaaa attttcgaga
    59941 ccagagaatt tcaagttcca tgaatttaat gctgtttatt ttacctgcca caaccagcct
    60001 aaattaacgg aaaaatttat attgttggaa ctgtcaaaaa aatctgtttt attgttggaa
    60061 aaaatatcgc tcaagctgta attggctcga gcatcaagct gcaaaacgtt ttttctgatt
    60121 ctgaacataa atcccaatcc tccacaaatg ccgtaatcga aaggattttc aacagatttg
    60181 acatgttgga ctttttcaga agaaacggtt tcgtttttta aaacattttc cgtaagtaaa
    60241 taactgattt taggcccaat attgaaaaaa aatcttgatt tattgccaaa ataaatatgc
    60301 gtcataaatg gaagttcaat ataattcagc cggcgactgt aaattgaatc ggtttctttc
    60361 catccacgtt gaaaaaagtt caattcaacc tgtaaaccta catttttttc ggtgatatgc
    60421 cggaatatca atcctccatt ataaccaagc agcatgccct gattgaccga tggcgaaaaa
    60481 ttgatcatgg aagcagatat tccctgagtg gttccaaaat aaatttccgg tttttcagag
    60541 ctgggttgtg cgaataaatt ggcagaaaat aaaaaaatac aaaggaaaat ggatattttg
    60601 cgcatgacat tatttggctt ttcgttctcc catataaagt ttgtcgttga tataaccgga
    60661 aaattccgat tgttttttga aatgaaaatc tgattgtatt ccttcatcaa aagttatttc
    60721 cttcaacttt ttagtaagat tattttcgta atttttcaaa atgggaataa aacagttcaa
    60781 caaatcgtat ccgagcaaat cataacgtgg tctttcactt tctacttccc attcaaagaa
    60841 ttgatgaaat gttttttggt attcttcaag agcggaagca tcgatttcag gtttaaaagg
    60901 agacacataa attctgtcaa acctttctat atgttgattt tcccagccat attcctgata
    60961 aagcgtcata tcgaactcat caatataaac atagaattct ttcaaaaatg gagtaattag
    61021 gcttgaatta tctgtattga aaaagagaag atttttttga tttcttttta aaacttcagg
    61081 aatactttga tttttgttag aagaagaaaa attaaattcg ttaaacttat gacgttcttt
    61141 caacagcctg tttttcaatc tttccataaa cagattccct tcatcaagaa tgctgatgcc
    61201 cggtagattc acaaaaacaa tattagcatt gcgataagtt ttctgaataa ttttatctac
    61261 aagaaaatct atctctattt gagtatcgtt gggattgaat tgaaaaagat agggattaat
    61321 gcctatttca tacacttttg aagaaaaagg aataagaacg tttattttgt tttcttttgc
    61381 aaaatcactt atcaacgaaa tttgattgct gtaagcaggc ccaacaatca aatccacact
    61441 tttcatttct ggttttgcta aaatttcttt caatttttcg tccgattttt cggtatcgaa
    61501 agtgaaaatt tccaatgaat aattattctc tttggccttt tttattgcca gtaagctgcc
    61561 ggcataaaaa tctctaaagc gtccgtttgc attttgctga gaaggagtgt caagattaaa
    61621 aggcaacaaa aaagctattt tgatattatt ttcaggaaaa agagccgtag taatttcagg
    61681 attttcaata tcttgcaatg cagaatccat ttcattgtta ttcagagttg ttgatggtat
    61741 cgaatctttt ttcaaaatag aagtcggaat cgttagttcc gtgccgattt tcagtacggg
    61801 aaaggcttcc ggatttagct tcaaaatatc atcaattgta acattatacg ttctgctgat
    61861 agaaaataat gtttcattgg gttttaccaa atgtttgata tactgaggtt tatcttttga
    61921 tggcagctgt ttttcgttca attccttttt tccacttgga gtaatcactt caactaagtc
    61981 tccttttatt tctgtctcat tttttgttgt ttgggtttta tatggaataa aaaggacgtc
    62041 tccttcccta agaatatttt tagattgtgg attatattgg attatttcct cttcactgac
    62101 attgtatttt ttactgatac cgaaaagtgt ttgatttttt tcaaccttat gctgtaagaa
    62161 taaagttgtt gaaggaaggg agttgtccgt ttgctgatga agattttttt gattttcttc
    62221 aaaagatttt tcttgcagta atgaaagctc atttttcaat ctctggcttg catcctcatt
    62281 ttcctgaaca tttttttgta ttggaatcaa aattttttgc cctgatttta gtcctttttg
    62341 tatttcagga ttaactttgc taatttcttc aggggcaatg ccgaatttgc gacttatggc
    62401 atacaaacca tctcccgatt gcaccgtgta cacatagtat tcaataccat tcactttttt
    62461 aatagggtag gagttggttt gttccattga aaaacccgta agcgacaata tgattacagt
    62521 cagcaaacag aagatgtttt ttttcatttg atggatattt ggagaaaaat acttgtttta
    62581 tgacaattga taaagtttct atgaatccgg ctgctaaacc ttgcaaaaat ataaaaaatt
    62641 aatgaaaaga ggaaagcagg caataaatca aaggtttttt tgtttttgaa aagaaaaatg
    62701 tacctttgcc aaactgtaaa tctgacgtta tcattcaggt tatatgaaaa aacttacgat
    62761 ttccttttgt ttctatctta ttgttttatc cgtttcggct caacttgttg caacagataa
    62821 atatgctgta tatcaaaatt ttacaggtgt cgattatatt ttcgttttta atggaatttt
    62881 acccggaacc gaaatacgct ttacaggaag tggtgataat gtgaagtggt acagattttc
    62941 tgatacgact acaccataca gtactttaca ctatatattt cctgaagatg caagcggata
    63001 tattttggat gtcgatggtc atcaaacttc tgtctgggtg attgattacc aaaattattt
    63061 gcccgttttt catacgctcg cttctgacga atcgagcgat aatcggtgca acgagttaac
    63121 tttattgctc gatgctaatg tgcctgaact gtattactat accttatcgg gcattcgtag
    63181 aaaaatcaat agaaatttca cgctcagtta ccaaactttg gaatgggaca acgaatggaa
    63241 aacagtcgaa acaacaaaaa acgttactct tccggttaca gagattactg tgccggctcc
    63301 tttgtgtaac accacatttt ctttgcaggg tgatcaattt acatccgaac catctttggg
    63361 tattcagcca atcggaataa tttcttcaat gtacgctgcc gtttctctgg aatgtcatat
    63421 aaaaacctct accacagcac gtacagaaaa aaatgaagat gaacgtccaa cttcagatgc
    63481 tttgagcggt tcagctccat tggaaatgct tttcaatgcc gaagtcaaca atgcaggaac
    63541 cattttttac aaatgggata tttataaaga caatcagttg cttgtttccc gttccgataa
    63601 agaacatcgt tatactttca cagaggccgg cacttataat gttaagctga cggtaaacaa
    63661 tgatttttgt tcgtcatccg attcggtatc gatttcagtc agcacttcag atcttcaagt
    63721 tccaaatgtg tttactccca atggagatgg aatcaacgac gaattcaggg tggcttataa
    63781 atcactgatt agttttgatg cgcgtattta caatcgatgg ggaagaatgg tttatcattg
    63841 gaacgatcca caaaaaggtt gggatggaac cataggtggg aaaaaagcgc ctgtcggagc
    63901 ttatttctat gtgataaaag ctattggtgc cgatggtatt aaatattcca aaaaaggcga
    63961 cattaatttg ttgagaggaa gagaaaatta aaatgtaaac ttttccgttc tccaaaaaaa
    64021 ttttcggaaa aaattcttga aatgtgcttt tcaaccatca atttttgaat gggttggttt
    64081 ttccatcaaa tacatactgc aaacaccaaa agtaaatttt ttgcattcaa ccaattcaaa
    64141 accattgttt tgaagaattt caatcatttc ctttcctttg gggaaagcgt gcatggaaga
    64201 ggtgaggtaa cgataggcgt tttggtcgga agaaagatat tttgccgttt ggtggacaaa
    64261 aagtttgatg taaagttggt atcctttcat caaccagcct ttttgaggtt cattcagttc
    64321 aagtatcaat aaatgacctc ccggtttcag cacacgaaaa atttgatgca agctttcttc
    64381 caatttttca aaatttcgta ccccaaaagc tatggttacg gcgtcaaaag agttgtcgtc
    64441 aaaattgagt tgagcgcagt ccatcacctt cagttctatt ttatccgaaa gttgggcatg
    64501 ttcaattttt tctttgccta tttcaagcat tttgggagaa atatctatcc ctgtaacttt
    64561 atcgggattc agcaaccgaa aagctttgat acacatatcg gcagttcctg cagccacatc
    64621 aagaatatgt ttggggcgga agcgctttag tgtacttaat gcacgttttc tccataattt
    64681 tgcaaaaccc cacgacatca aatcgttaaa tttatcgtac tgggcagcta tggtgtcgaa
    64741 catgcgcacc aattgttctg tttttccttc tttttcgttg tatggtttta cttcctgaaa
    64801 agtatattgg cttgtggttt tttctttatt catttgaatt gtaatttgtt tgcaaaatta
    64861 attacaaatt tcggtaatca aagcatttca ttttatcaaa caataaccaa agaaatttca
    64921 cttatctatg aatctatcag tttcttaaaa gtaaaattta aattttcgtg aaaagttgat
    64981 gaaaaaaatt ttgtgttttg accttatttt gtaattttgc aatcgattat tggtgtttat
    65041 tttttgttct gaaaagtaaa caaaagggaa tccggtgaaa gtccggaaca gtacccgctg
    65101 ctgtaagctc ctttataacg tactgaacca tactttggcc actttttcca caaaaagaat
    65161 ctattttggg aaatgggaag gcgttcagat acgggagcaa gtcagaagac ctgccaatat
    65221 catttttgct tttttgcttt cgggaataaa tggcaaagaa agtcgaaaca tatctccctg
    65281 attgagaata tattttttca tctgatgtgt ttttcttctt ttttattttc atgaattttc
    65341 cttaacaaat ttagcaagtt tttgtagtcg aattgtttaa ttttaaaatg gaataaaaat
    65401 gaaaaagaaa agtttgattg ttttatctac attattgttg atttttttta cttcttgcga
    65461 aaatgattcg gactttaagc tcaagaccac tgtagatttt gaggatgttg agctgggaga
    65521 aaatggttat tggaatggat ccgatttttc gaagaaattc gtttccaacg gttttatttt
    65581 tcttaatgat tttacttcat cgccctgggg cgatttttgg tcggggtttg cctgctcatc
    65641 tttaaccgat accattactg ccggttacga taatcagtac agtgtggctg ccggttcagg
    65701 tgcatggggc tcaaataagt ttgctgttgc ctttgatgat tcggccactg tgaattgtcc
    65761 gaaaaatgaa ttgggaactt acgaagcaaa aagtatcatg ctttgcaatt caacttatgc
    65821 atatctcgat atgcgcaatg gatcggcttt cagtaaaaaa tttgccgatg gagactggtt
    65881 taaggttacc ataaaaggct ttttgaataa taatgctaca ggtgcggttg attattattt
    65941 ggccgatttt cggaacggga aacattatct ttgccatcaa tgggtaaaag ttgacctttc
    66001 ttctcttgga aaagtagatc agttgatttt tcttttcgat tccagcgata aaggaacttt
    66061 tggagtgaat acaccaaaat atgtttgcat agataatctt gaatttgagc aagatgctga
    66121 aaattaattt ttcaaaagac tttcaacgta tgatattgct tttacctgcc ataatatcag
    66181 catttcatac ttttgagacc gggtgtaaaa tcccggtttt tttctttttg gaagtttgac
    66241 ttttagagaa attgctcctg tttttgaaat gaatgatgtg cagcattaaa atattcaact
    66301 tgaaaaaact cttcaataga ttaataaaat tcaattcttt tgcaataaaa tagataaatt
    66361 caaaaactta tcgctatttt tgcatagtac aatagtgaaa aaatggttat caaataatta
    66421 aatgattgat aatcaaaatt taattttgtt tccaatcaag taacacctat ttataaacga
    66481 catggaatat acaactttgc agggaaaact gatcatgcct gaatatgggc gtaatattca
    66541 gcagatgata gcttatgcca tgacgataga agacagggaa gagcgaaccc gttgtgtaaa
    66601 aaccattatc aacattatgg gaaatttgtt cccatatctg cgcgatgtga acgatttcaa
    66661 acataaattg tgggatcacg tggctatcat gtccgatttt aaactggata tcgattttcc
    66721 ttatgaggtt cttcggcctg aaaatcttta tacccgtccc gaaaagattc catataaaaa
    66781 ttcagatatt cgatatcgac actatggccg cacgttggaa gaaatgatag aaaaagtagc
    66841 cgattatccc gatggagaag aaaaaaacga attaatacgc ctgatagcca atcagatgaa
    66901 aaaatgtttc cttacatgga ataaggaagt agttgatgac cgaaaaattt tcgacgattt
    66961 gcgtgaatta tccaaaggaa agctcgatat ttcggaagat tttctgaagt tggttgaatc
    67021 gaaagaagtg ttacacaaca agaaaaataa aaagaaaaac aaatagtttt cttttacccg
    67081 tttccttttt ttaatcatca tccaaccaca aattatatgt cgtcatttca aatagaaggc
    67141 ggtcatcgac tttcgggatc aattacaccg cagggagcca aaaatgaagc gttgcaggtt
    67201 atttgtgcaa ccttgcttac atccgagtca gtcattattc gaaacattcc cgatattctt
    67261 gatgttagcc atttactgag tctgttaaaa gatttgggtg taaaaatttc aaaaatagaa
    67321 aagggaactt attcgtttaa agccgactcc ctaaacatgg actatttgca atcggaagat
    67381 ttttatgaca aaagttcttc gctgcgtggt tcggtgatga ttttgggccc gcttctggct
    67441 cgatttggaa aagcgttggt tccaaaaccg ggaggagaca aaataggacg tcgacgtttg
    67501 gatacccatt tttcgggcat tcagaagctg ggtgccgatt ttcgatacaa tgaaacaaaa
    67561 aaacagtatg aagtatctgc aaaaaaactg aaaggcacct acatgctgct ggatgaagcc
    67621 tcggtaaccg gaacggcaaa tattttaatg gcttcagtgc tggccgaagg taaaactaca
    67681 atttacaatg ctgcctgcga accctatatt caacagcttt gcagaatgtt gatagcaatg
    67741 ggtgcaaaga tttctggagt tggctccaat ttattgacta ttgaaggagt gaaacagttg
    67801 aatggctgcg aacacactat tttgccggat atgatagaag ttggcagttt tatcggcatg
    67861 gcagccatga ccggttctga aatcaccata aaaaatgtgg catacgatga actgggcatt
    67921 attcccgaca gttttcggcg aatgggtatt caagtgcttc agaaaaatga tgatatttat
    67981 attccggctc acgaaagcta taccatcgaa acatttattg atggctccat aatgactatc
    68041 gctgatgcgc cctggcccgg actgacaccc gatttgttga gcgtttttct ggtaattgct
    68101 gcaaaagcga acggaagtgt tttgattcat caaaaaatgt ttgaaagcag attgtttttt
    68161 gtagataaac tgatcgacat gggtgcgcag attattcttt gcgatccaca tcgggcaacc
    68221 gttatcggac tgggaaatca gcttcgttta cgggcatcgg taatgagttc gcctgatatt
    68281 cgggcaggaa ttgccttgct tatagctgca cttgcggccg atggaacaag tattattcac
    68341 aatatcgacc aaattgacag gggctatgag catattgata cccgtttgaa cgctttggga
    68401 gcaaatatcg agagaatcca attttaaaaa tttcatatac ttaattttag ggaggaaata
    68461 ttatgctgaa cgataaaatt accgaaatgt tgaacaatca aatcaacaag gaattttttt
    68521 catggtatct gtatttggat atttcaaatt attacgccaa caaaaatttg gacggatttg
    68581 ccaactggtt ctccattcag gcgcaggaag aatatgctca cgctcagcta ttcctgaaat
    68641 atattttaaa caatggtgaa aaggtcaaac ttttacctat tgcagctccg gaaagtaatt
    68701 ttgtggattt taaagctcct ttgtcggctg cttttgaaca cgaattgttt atcacagctt
    68761 ccatcaacga aatttatgcc gaggcatacg ctcagaaaga ttttaaaacc atgcagtttc
    68821 tcgactggtt tgtgaaggaa cagggagaag aagaaaaaaa cgtggacgat ttgtgcaaac
    68881 ggttcgacct tttcgggacc gatccacgcg gtttgtattt gcttgataac gaattggctt
    68941 cccgtgtata tactgctccg tcgctggtaa tttaaagaaa ttttttttct gctgaagtta
    69001 aaccactcca atcaaatgag ttaagggatt attttgcaac aaaaaatttt ctttattacg
    69061 ttgaaactta tcggaatttt cacctcaacc agaatatagg tttggggttt tgttgattta
    69121 ctcttttgac ttttaaagga tggcttaatc attcaaaatc gacaaattgt agtatagaca
    69181 gataggtatg ggtaaaaatt agaaggcttg gcaggtatta acgatttgat atcgaatggt
    69241 tttctcctgt tagaaaaaaa cacaacaatt tggttcatag taactatgtt ataaagtttt
    69301 ttccaccgta caaattctga ctaaattgaa attaaaattt tccatcttat tatttggaaa
    69361 aacagttgct ctgtccattt ttcacaacaa tgatttcact cattcaactg atatagtatt
    69421 tgggattgta aaaacgaaaa aacacattgt tttttagaat atgaacagat gaaatacttc
    69481 tataggaaca tacaagaaaa aggatgaaga ctgtcgtcaa ttactttgta gaagaaaacc
    69541 tacggaacat atctaacgca aaagccttat tactatcgac agagttacct ctggatttag
    69601 cagaaaggaa tgcataacat tagaagaatt taataaaaaa atgaacttga attggttttg
    69661 gttattaaaa cggtttggaa gtgagcgtag aactaaatct gtaaattgct aaattatccc
    69721 gtacatttgt aaaaacgatt tttttgaaac tgtgagaatc atgaaaaata ttttgtccac
    69781 tgttgttttt atttttttat ttgttaattc tagtttaaca gccgccaatc cctatcgaac
    69841 caaaattttg aaacccaata tcaaaacttt gcaaatcaga atttcggata ccgacttcgg
    69901 tttccccatt ttgaaattaa atggaacaga aacacttact gtggagtttg atgaaatgtc
    69961 gcacaatgtt cgctctttcg gttacacggt tttgcattgc aatgctgact gcacggtttc
    70021 caatttaaac agcaacgaat atgtcgatgg ttttacgcgt ggtaatatcg acgattacgc
    70081 cttttcgcaa aatactactt tccaatatat tcattatcgg tttacgatac cgaaccaaga
    70141 catgcgtttt aaaatttccg gaaactatgt ggtactcatt tatgaagaca atcgtgtgga
    70201 tgatccggta gccactgcct gtttttccat tgttgagcca aaagtagaga tagatgcccg
    70261 tgtcagagcc aataccgata tcgaattgag tggacgctat cagcagctcg acatagaagt
    70321 gaatctggca aattatgttg ttcaggatcc tgcttcggaa gttaaactcg ttgttcgaca
    70381 aaataacagg ttcgataacg aggtggcaaa cataacgccg acttttttca atgccgggaa
    70441 gttaagtttt gtcaataaca aagcattgat ttttgaagga ggaaacgaat accgtcattt
    70501 tgatatttcg tctttttatg cagcaggaga gggggtggac agaattattt ttaatcgcaa
    70561 gtattatgaa gcttttctta aggaagattt gatgcaaaag tcgcgtacct atatttcgca
    70621 acctgatgcc aatggccgat ttatcataaa ttttcaggaa gcttttcagg acgtacaaac
    70681 ggaagccgat tatgttcagg tacatttttc tttactttcg gaacaaccgt tttttgatgg
    70741 acaggtattc attggtggcg attacaatta taatttgctg gatgatacag cgcgaatgca
    70801 atatgatttc gatgctcaac gttataccaa aacgcttttg ctgaaacagg gcggctacag
    70861 ttatcaatac tggtttttgc caaaaggtaa aaattctgct tcaaccggaa aaattgaagg
    70921 cagtttctgg caaactaaaa acgaatacac catttatttt taccatcggg cttggggtga
    70981 acgttacgac cgattgatcg gttacagcgt aatcgactga ctataatgat tgaacaaaac
    71041 attttatttt gtaacgatgg ttttgttgga agcaacacca gtttttttgg atgatggagg
    71101 ttttttgaag tttaatttta ttgacctacc tttgtataag ggaaatttca tcagaatata
    71161 atttattttt taaaggtctg atggaaccca cctgtctgaa tattatattc attcgtgttt
    71221 gtgtatgcaa tacatgtggg attcatattt cagaaaaacg ataaatataa ttgtttggaa
    71281 ggcaattaat tatttttatt aagttaaata tcagatttct acatgaatca ggaagacaca
    71341 tttccgacaa ccgactccct aaaagatttt caacaaaatt ttaccaaaga accgacaatc
    71401 gaaaacctga aaaatgcttt aaacaatgaa attttcaaca ttatttcgga tattgccgat
    71461 gaagcacatc aggaatgtta tgtaattggc ggtttcgtgc gcgatttgct gctgcgccga
    71521 ccatcaaagg atattgatat tcttgtggtg ggcaatggta ttgaattggc agagaaagtg
    71581 gtcaatcaac tgaagcaact gaacaagccc tgcaaatttg ccatttttaa aaattttggt
    71641 acggcacaaa tcaagctgaa agaaatggat gttgaatttg ttggcgcccg caaggaatcg
    71701 tatcgttccg attcgagaaa accgattgtg gaagatggaa ctttgcagga cgaccagaat
    71761 cgccgggatt ttaccatcaa cacccttgcc ctgtgcctga ataaaagtcg tttcggtgaa
    71821 ttagtcgatc cattcaacgg tctcagggat ttgcaaaaca aagttattcg cacccctttg
    71881 aatcccgata ttactttttc cgacgatccg ttgcgaatga tgcgatgtat tcgttttgcg
    71941 acacaactca atttttttat tgaaaccaat acttttgaag ccattggtcg gaataagaag
    72001 cgcatcgaaa ttatttcgaa agaaagaatt acagacgaat tgaacaaaat tattctttct
    72061 cccaaacctt ctatcggctt tattttgctc gacaagtcgg gtttgttgga ttttatttta
    72121 cccgaacttt cggccctcaa aggtgtagaa accaaagatg ggattgggca caaagatatt
    72181 ttttatcata ccctcatggt gttggacaat ctcagtcaga cttcggattc actttggctg
    72241 cgatgggctg ctttgcttca cgatatagga aaaccgcgca ccaagcgctt tgacaaacgt
    72301 cagggctgga ccttttataa tcataacata gttggtgaaa aaatgacagc cgatattttc
    72361 agaagaatga agcttcccac caacgaaaaa atgaaatatg ttcagaaaat ggtttccctg
    72421 cacatgcgtc ccattgtact cagtgaagaa gaggttaccg attcggccgt cagaagactg
    72481 ctttttgatg cgggcgacga cattgacgac ctaatgctgc tttgcgaagc cgatattacc
    72541 tctaaaaatc cggaaaaggt aaaacgttac agggaaaatt ttcagctggt tcggaaaaag
    72601 ttgaaggaaa tagaagaaaa agacaggata aggaattttc aacctcccat tgatggaaaa
    72661 gaaatcatgg aaacttttca tcttcctccc tgtgctttgg ttggagaaat taaaatgaaa
    72721 attaaggatg ccattctgga tggtattatt cccaacgaat atgaagctgc acgaagctat
    72781 atgatgcaga ttgccgcaga aatgggatta accaattaaa aacgttataa aaatgaaaaa
    72841 aattttttat tttattccgc tgttgctttt gtcatgtgcg acaatggcaa aaaataatgg
    72901 aaatcctctg aaatttaagc attccgaaag aagttttgct gctttcgata ccgaacgttt
    72961 ggctcggttg gacactgttt ttcaggattt ggtaaggaaa ggcgaaattc ctcatgctgt
    73021 tacttttgtg gcacatcgtg gcaacattgt tcatttcaaa gcatttggct ggcgcgataa
    73081 ggagaaaaat attccatgcc aaagagacga tatttttcgt atggcttctc aaaccaaagc
    73141 tattaccgca acaggtgtgc tgatgttgat ggaagaagga aaaattcttt tggatgatcc
    73201 ggtaaaaaaa tatattcccg aatttgccca tccccaagtt ctggtgtcgt ttaacgaaaa
    73261 agattccact tatactacca ggcctgcaaa caaggatatt accattcgtc atttgttgac
    73321 acacacatca ggaatttctt atggaacaga tcccaataac cctatgatga aaatttttgc
    73381 taaaaaggga attccaccct tgaacagctt ggatccggtt acattgaaag aagtggtgaa
    73441 aaaacttgct tcctgtcctt tggagcacga tcccggagaa agatttacct atggaatgag
    73501 cgttgacgtg ttgggttatt tgattgaagt ggtttcaaaa atgcctgttg accaatattt
    73561 taaggagaaa atttttatgc cgcttggaat gaacgatact tatttttatg ttcctaagga
    73621 aaaacaaaac cgtatggtta cactttatca aaaagtgcag ggcaaaactt tgcagcccag
    73681 ttcaagtgtg ctttatcaga catttccgta cagtggcgct caaactctgt tttcgaccgg
    73741 tgccggctta tcgggaacga ttgaagatta tgcaaaattt tgtcaaatga ttctcaacaa
    73801 gggagaattt aacgggaaac gcatactggg aagaaaaacg gtcgaattga tgtgtcgcaa
    73861 tcaggttgga gatttacgcg gagaaattgg ctttggtttg gcattcgacg attttcgtcc
    73921 tcaatatttg catcttacaa tggcttccga aggttcttta cgttggggtg gcatgttcgg
    73981 aaccgattat cttatcgatc cgaaagaaga tttgatttta cttttttata tcaattggca
    74041 aaccgataac agtgtacccg atgtcaaagt tttgttccac aatttggtct atcaggcatt
    74101 gaaatgattg cataatattt ttataatttt acagtaaaat gaaagcagtt tttatccttt
    74161 ctttttccat tgtttttatg gcagcttgtc attctttttc aaaaattgct tatccacata
    74221 cccaaaagga aaacgtaacc gatcattatt ttggcactga tgttgccgat ccatatcgct
    74281 ggctcgaaaa tgatacgtcg gcagcaactg cggcttgggt aaaagctcag aatgaagtta
    74341 ctttcaaata tctggaaaaa attccttttc gaaatgcgct gaaaaaccgt ctgactcaac
    74401 tgaacgatta tcctaaactg ggtgctccgt ttaaaaaaca cggcaagtat tattttttca
    74461 aaaataatgg attacaaccg caaagtattc tttatcagca gtcaacctta tattcggaac
    74521 ctgaaatttt gctcgatccc aacacttttt cttccgatgg tacggttgcc ttgtcatccg
    74581 tttcgttttc aaacgttggc aattatatgg cttattcggt ggcaaaaagc ggttctgatt
    74641 ggaatgaaat ttttgtctta aatctggata accgacaaac tttatccgat cacattcagt
    74701 ggacaaaatt ctggaatgtt gcatggaaag acaacggatt ttactatata gcctacgatg
    74761 ctccgcaaaa aggcaaggaa ttgtcggcaa agaacgaatt tccaaaagtt tattatcacc
    74821 agataggaca acttcaaagc aaggatttgt tgatttatca ggataaaaat catccgttgc
    74881 gcgaatttgg cccatttgta acggaagatg aaaaatatct ttttttgatc attacggaaa
    74941 caaccaccgg caacgctctg ctgatgaagg atttaaccga tcctaaagct aaatttgttt
    75001 cattggtgga tggttttgaa aatgattatt cggtcatcga ccacataaac ggcaaaattt
    75061 atattcttac caattggaaa gcgcccaata agcggattat ggaagttgac ccggcccatc
    75121 cttcgcgcga aaactggcgt gaaattattc ccgaacaaaa aacggtactc gaatcggttg
    75181 aaattatcga tggaaagttt ttttgccttt atctgaaaga tgctgctcat cgtctgtatg
    75241 cttacgattt aagcgggaaa cttctttacg aagtaaaatt gccggcactt ggctcggttg
    75301 taagcctgac gggaaacaaa gacgacagtg aggctttttt tgctttcagt tcttttacct
    75361 atccacctac catttatcgc ttggatgccg gcaagaatgt ttccgaaatt tttcaaaaag
    75421 cgcaggtgaa atttaagccg gaagattttg ttaccgaaca ggttttttat acggctaagg
    75481 acggtacaaa aattcccatg tttctttcct atcgcaaggg aataaaaaaa gacggaaaaa
    75541 atcctttgat gctttatggt tatggaggtt tcaatatcag catgacgcct tctttcagta
    75601 tttcgcgtat tccttttctc gaaaatggag gtatttatgc cgttgccaac attcgtggtg
    75661 gcggagagta tggagaatcg tggcacatgg caggaacaaa aatgcagaaa aagaacgttt
    75721 tcgatgattt tattgccgcg gcggaatttt taattgcaga aggttatact tcttcaaaaa
    75781 aactggccat taatggtggt tcaaatggcg gtttgttggt aggagcttgt ttgacgcaac
    75841 gtcccgattt gtttgcagtg gctgttcctg aagtaggtgt gctggatatg cttcgttacc
    75901 atttgtttac tattggctgg tcgtgggcaa gcgattacgg tacgagcgaa gaaagcaggg
    75961 aaatgtttga atatctgaaa tcctattctc cgttgcataa tgtggtaaaa ggcacaaaat
    76021 atcctgcagt tctgattatg acaggtgatc acgatgaccg tgttgtgccg gctcattcat
    76081 tcaaatttgc tgctactttg caggaagcca atgccggtaa taagcctgta ttgattcgcg
    76141 tggataacaa tgcgggacac ggagcgggga aacctttggg aaaactaatt gatgcacaaa
    76201 cagatatgtg gtcgtttgta atgtataatt tgggaatgaa gcccaaatgg tgaaaaggtt
    76261 tattaggtaa ggaagtaaaa atcagatttc aaaccatgta ggaaaactta tttttttctg
    76321 cgggttttga aaatggtcaa aagaagtcct aaacccatga tgccggccaa caagtaaccg
    76381 ataacgccca aaatggaaat atcggaaatc agggggccga ttctttgttg caaaattaac
    76441 gaagaaccta caatcagtgc cgcaataatc aaactggccg atattctgtt gctcgaactt
    76501 ttcatttcat cctgcaaatt ttcgaggttt tgatgttcca gtttgatttc aaactgtccc
    76561 tctttcaact ttccaagcaa atcggatata ttttgtggaa aaagttctat tagttcaatg
    76621 ctctcatcca tcacgttcat gctgcgtttt ctgaaacttt ttggagaata gagtttcaga
    76681 taaattcttt tcacaaacgg ctcaacaatg gcaaacatat cgaagtcagg ctccagttgc
    76741 tgcccgattg attcggcgat agacatggct cgcagcatca tcaccaaatt ggaaggaagc
    76801 gccatgtcat gacgagccat aatttccagc aactgctggt tcatccggtt caggctaatg
    76861 gttctgaatg gtctgtcggc atacatttcg atcaattcgg tgaggtcgtg ccgcaattga
    76921 cgcttgtcgg tttctttaac cacgattcca agttcttgta agcccttgat tacgtgattg
    76981 ctgtcttttt cgttgacggc acgtaaaatt cgggcaatgg taatgatgga ttcttcgtcg
    77041 agatgtcccg tcatacccac atccaacatc acgatggtgg caggaggttg gataaaaaga
    77101 tttcccggat gcggatcggc atggaaaaaa ccatctatca gaatttgttt cagcaatgct
    77161 tttgccgctc tgtcggcaac ttttttgggc tcgtagcggt gttcgtattt ttcctgtgtg
    77221 atttcggtaa ttttcactcc ttcaatgaat tccattgtca gtaatttctc ggtagtaaat
    77281 tgccaataca cttttggaac ttgtatgtaa tccacatttt tgaaattttt cctgaatttt
    77341 tcaaagttgt gggcttcatt catgaaatcc agttcttttc gtatggtttt cttcagttcg
    77401 gccacaattg cagttggatg atacagggaa ctgttgcgaa gtcttttttc cagtatttcg
    77461 gcaaagcttt cgaggatgga gaggtctgtt tcgatggttt tatgaatatc aggccgttga
    77521 attttcaggg ctaccgtatc gccattttgc agaactgctt tgtgcacctg acttaaagaa
    77581 gcggaagcaa ccggcgattg gtcgaaatcc aaaaaaagtt cttcaagggt atgtttcagc
    77641 tctttttcaa tacaccgcct caccagcgga gtttccatag gtaaaacctg atcctgaagt
    77701 ttttccaatt cacgaatata ttccgcagga agaaaatcgg gacgggtact gagtatttgc
    77761 ccaaatttta tgaaagttgg gcccaattct tcaaaagcca gacgaagcct ttcggcagtt
    77821 gaaagtttgt gataaacttt tcgtcgtttc ctcaattttg aaagaaagtt cactcgagaa
    77881 gcttcaaaaa gataatccag accaaatttc acaaaaacgg aaatgatctg acggcttcgt
    77941 ttgactttat ccaagttgtt tctgaaaccg aaattcctgt atggatagga atcgttcatt
    78001 ggtttaattt tctgattctt cctgtttttt gttgagcagt tgcgtcaact cttctacttt
    78061 gtttttcagt tcttcgaatt cttttcgtga aggaatattc atttttttta gaattgcatc
    78121 gactgccttt tcaacctttt cctccatttc ggttttggtt ttttctgatt gttccagcaa
    78181 atctttgata aatttaggtt tctccgtttc ggttaattct ccgcgtttaa tcaggtcttt
    78241 agcaaattct tcggcttttt ctcgcgtaag cgaaaccaat cccagccctg ccaaaaaagc
    78301 atttttaatt acgtctgcca tagtgatatc ctcctgtttt tagaatattg atgatgtttt
    78361 tctttgaaaa gttttaattg gaaaaaatgt cggtatccga cttcaaactt aagatattag
    78421 ttttgaattt acaagatttg tactaaaaat ttttatcggt ttcattcagc gatttcgaag
    78481 cgttgtttga tgaatgaaac aaggtgggcc aacggcaaga atttgggatt cggagcaatg
    78541 ttagcggtaa attattcgat ctacaataat tgattgttgg ttggatgaaa aagaaaactt
    78601 acagattcct gtaacctgct atgtttcttg gaataggtgc tgattttcca ggtcgatgga
    78661 atttgcaatt tcatctataa ttatcaggaa ttgaattttg aaaaaaataa acgtctaagt
    78721 aattagcaat actggattcc aaatcctcgc ttcgatggct acaacccttg atattaacca
    78781 cttaccctgc cattacttat acaatagttg ttggctggtt ttttgacctt ttagaaggct
    78841 ttctcacatc tttctataca ttatacattc gatataaatc ggataaagtt atcttgtaag
    78901 catctaaatc tttttttcgt ttttcaaata tttcagtaat tttcgctttc cgaatcgatt
    78961 gttcagagta atgtatcatt ctgtgacaat ttggacataa agcaataata ttccttgcat
    79021 tatcaagtga gtatttgaaa ttttcttgct ctttcatcgg aatcaaatgg tgtgcttcaa
    79081 cataattttt tttgtgtaat ttgcaataaa actttcatgg ttatctgatt tagtagcaaa
    79141 ttcacaaata tagtttgcgt gtttcagagc tgcttttgaa attcttgaat cccgaatcca
    79201 tgtggttgaa ttacttatct gcttggctgg tctgagtttt aaagcttctt taatcggcat
    79261 atccaccgag tttaaatcgg aatcaacatc atcataaaca tctaaaaaag caccgtgaat
    79321 aattgacatt agaagattat actcatcaag ggttaaagga attacggttg tatttagctt
    79381 accattatta cttaatttat tgtcaaaagg ttttatttgc cgtactctgc caagactcat
    79441 aggactttgt aaatcgcaga tttttctaat agcaatttct tttttatttg gatcttgtga
    79501 aagagatttt tcaatcactc caattgaaac aaaagatttt gatttccctg agaaatatcc
    79561 aattacttta tcgcctactt gtagctttga aaaatttctt ttgagtctct ttttccatat
    79621 gcaggccaat tttcaatttc accaactttt tgattggttc gccaatccca atcgttgtca
    79681 tttgcaataa atatccaatg tttcattttt tttatttatg attgattgtt taatatcaca
    79741 ttatctaaac acattcttaa tcacatcaat tttcaccaat ttattcccgt taaaatgcaa
    79801 atactcaaac gggccatcta cttgcccgcg gaccagggta tgggcttctg tctgtccgaa
    79861 attatgttgc ctatttttta gcatcatttt tccttttttc aatgaatgca aaaagttttt
    79921 ctttataatc gtcaaaccgc ataacattgt gttgtccttt tggtgtccga atttttttcg
    79981 gaaagtttgt tgcttttttt atttcatcaa aagtgattct caaattatcc aagtcagccc
    80041 aatgattttt gtcagcaaca acataatttt tgtattgatt agctgtaagt aattcactac
    80101 tttttaaaca attgtttgcg gctatacatt tctcaaattg tttagaagtg caatgaaaaa
    80161 ttcttctaca cttccaattg tccgtttcgt ggtcgtcagt ttggctttga taaaaagttc
    80221 cactacctgt atcaagttgg attaagaaat ttggcgttgt ttgtccgtgt gtcttgtagt
    80281 aacttttctg taagttttta taacgccatt tgatggggtg gcttgaaatt gttttagaaa
    80341 taataacatt ttgtcttgaa ggaaattgtt gtaaagcaaa gggatagtcc aatttattgc
    80401 caaccttaaa ccatcctttt agaaaatagt cgttgtcaac ctttgcaata aaaattaaag
    80461 tatctccaac tttcacactt tttcttgtcg atggtctgca aatgccccaa cttggtggac
    80521 agtcaaaaca tgggtcgtca ccaatgtcaa cgattttgtc gttaacgatt tgtccgtgaa
    80581 atactataag ataactttta ctcattgcac tttctgtctc gtttgcatta tcgtttagcc
    80641 tgacccctaa cgcccgagtc tatggacatt ggcggcttgc gggagcgttc actgtcgcca
    80701 agctacaaag tgaatgcggg agcaaaacct ccaaattaca cgtcagccag cctgtgacca
    80761 taggcgtgtg ttagcagcag tggatttagt ctttaatcgg ttagtatatt ttcaaaatac
    80821 tcaattgttt ttttcaatcc gctttcaagg tcaatttttg gttgccaatt caaaacttct
    80881 ttcgctaacg aaatgtcagg acagcgttgt ttcgggtcgt cttgaacggc tgaaaagaat
    80941 ttgatttttg atttagaatt tgtcaaatct attatttttt ccgctaattc caaaattgta
    81001 aactctgttg gattgccgat atttacaggc gaaacgaagt tgtcacgcgt gttcattaaa
    81061 cgaatcagtc cgtcaattag atcatcaaca tattgaaaac ttcgggtttg ttgaccgttt
    81121 ccgtaaatgg ttatatcctt gttttgcaat gcttcaacga taaaatttga aacaactcga
    81181 ccgtcatttg cttgcatatg agttccgtaa gtgttgaaaa ttcttgctat tttgatacga
    81241 acattgtttt gtcggtggta atccataaat agcgtttcgg cacagcgttt gccctcgtca
    81301 tagcaagaac gaataccaat cggatttaca tttccccaat aactttcgtt ttgagggtgc
    81361 gcaattgggt cgccgtagat ttcgctcgtg gaagcctgta aaattttcgc atttgtccgc
    81421 tttgccaacc ccaaaacatt aattgttcct aataccgaag ttttgacggt ttttatagca
    81481 tcgtattgat aatgaatagg cgaggcagga cacgccaaat tataaatttc gtcaatttcc
    81541 gtaaaatacg gctcgcaaat gtcgtgttct acaattttaa aatttgggtt ttctattaaa
    81601 tgattgatat tttgcttgtt acctgttaaa aaattatcta aacaaattac tttatttcct
    81661 tcgtttagta atctctcgca caaatgagag ccgacaaaac ctgcaccgcc tgttattaaa
    81721 atttgtttca tactgctgtt atttcttctg ttttgtcaaa ataaacgtaa tattctggaa
    81781 tttcttgtaa taaagtaact tcgccctgtc cgtatctcat gaattgacga gtaaatgatt
    81841 ttaggttttt ccaaagtccg tttgtgtaac cgtaattgtg aaaaacaatc atttgaggaa
    81901 tgtgttttat cgcaaaacaa tcgtgatttg cccgaaaaca aaactcaatg tcttcgcctg
    81961 cggctaacga aaactcttcg ctaaaatcaa tattttcagc aacttgtcga gttgtagaca
    82021 aatttgctgt tgttccgtac aaaagtcgct cactgtcttt gaattttcga ccgttcaaag
    82081 ttccgttaat attgtgatat attccaaacc aatttttatc ataagaaatt gtgttgcccg
    82141 acaatatctg aaaatctttt gactgctgaa atgattttat tatcgtttca acccaatttt
    82201 ctgaaagtat gcaatcggta tcggtaaaag cgataatgtc cgatttattt tcaacagcat
    82261 tttttttggc ccaatttcgt gctttcgctg gtcccgaatt tttggtttgt cgataaactt
    82321 gtatgtgttc ggggaatgaa taagaaattg gcgaaaaatc atctacgata ataatattgt
    82381 cgggttttac cgtttgcttt gaaatagaca gcaaaagatt ttcaatgtca tttatatctt
    82441 tttgatttct aataaaagtc ggcacaacaa tgctgacaat cggattttcc gaaatgtcgt
    82501 ttgcgattgg ttgcagaaaa tctacatatt gataaatttt attgttttgc ttgctactat
    82561 gccgtttgaa ggcgattatt tcaataattt tagtccataa gaacttcttt ctgtatggat
    82621 tatgcgataa tttttcgtac caataatata aaatcttgcc gtatggaaca tttgataaat
    82681 aactcatatt ttaaactcct aaatttgcac aattctgaca ataataatga cacttgtgag
    82741 aactcaaaaa aatttttctt tctttacttt cccatatttc acttaacgat tgattaaaaa
    82801 tatttccgta agtgtatgga ttaaacatat tacagggtgt tgctgtaccg tcgtacaaaa
    82861 tgcacaattc tttttctaaa gcaggacatt gtttcattgt tccgtgcgtt ttagtcggtt
    82921 tttcggtttg caattcaaca cctgcgagtt ggtcgggata aaaaaattgc aattctgaat
    82981 tagcgtataa ttttccgatt tgcttaaatt gttctaaaat tgactgataa ttttgtttga
    83041 caatttcaat aaatacttca acgccttcat ctctgcgaat ggtgttatac aaaatttgac
    83101 aattattttc aaacgcaaac tccgccattt caaaaatacg gtttatattc gcctcttgta
    83161 tagtaaaaat aacaaaaggg cgattgggca actcttggag ttgcttgata ttattttcaa
    83221 taaattgaaa atttgctcct tttctgattg ctgacaattc atctgctttc gggctgtcca
    83281 ttgaaataaa aagttgaaca ccatttgcaa tcaaagcatc aataattttt ttgttattga
    83341 atgaaaaatt tgaaaacaaa tttatattga gttgcggaaa atgttgtttt gtgtaattca
    83401 aaatctcaac aaaatcgggg tgtattgtgc tttctccacg accatttagg cggattgttt
    83461 ttgtcttgtc gccaatttgg tgcaaaattg ttttgtattt ctcaaaagac aaaaatttgt
    83521 ttttattgaa tggatttgcg tctataccaa atccgcacat tattcaactc atattgcagt
    83581 tgtaacttaa ttctatgatt atttcttcaa atcgcataag ttatttcgtg ttgcatttta
    83641 aaaccttgca aggcaaatta caaattttca ttttctcgca aataaataaa gaataatcgg
    83701 taagcacctc aggtcgctgg aatttgcaat cccagctctt tagttattag gatttttaat
    83761 cctgattaat taacagggtt caaactagcg ttacatccat taccgtgttt gttaaaaaat
    83821 acacttcccc tgttatgcgg ttctgtatat tcatatcaca tatatatgaa aattgactgg
    83881 aattatgttt aaatcggatt ataaatcctt atagaatcta aaaacccgca ctatctcggc
    83941 aataaaatat accgcctgtt atgcactggc ggttcattgt ttgctcaatt cactaaagag
    84001 tttttgtaat ttatatgttt tagttgcttt gttactttct acaataaaat tttctttatc
    84061 aattctatat gatttgaaaa aattatcatt acatcttaat ctgccagtaa atcctttttc
    84121 aatatctata atgaaaatag gatttgaaaa tgcctttgag ttaatcaatt gtaagataag
    84181 atatttatca attaatacaa cgtcttcaat agatttccat cttaattttt ccggcgctcc
    84241 agtaagttgg tagtcagtaa caaaaaccca ggtatgtaaa ttcccttttg ttacaactac
    84301 accatgaaat gttcgaaaca ctttatattt tgtggaagca tcaactaaaa ccaaactgtt
    84361 tcccttttga ttttgataat gattatgagc ataataaact aaccatcctt tattcttttt
    84421 tggattatac catgtgggtt tttcattatc agcaaaatac aatttttgta gagtgtctcc
    84481 cgaaacatca atatagtaag tgttgggtag ttctattttt cctggaatcc attgaggtgg
    84541 ggtttttatg aacaagtcgt aataactgtt atttgataat ttcttgttat ttatatttgc
    84601 cgaaatgagc cattgcaagg aagcatctgg gaaatttctt cttgcaggta acaattcttt
    84661 tttaatcgct tctaaaaacg gtttatttct taattgcatc agttttggag gaattaaaat
    84721 aattttcttt atgtcgcaaa aacaaccaaa atcattaagt tcaaaaattt cttttgtctt
    84781 tccatttatt aagctgagat aagtgccgcc aaaaccactg ccaccgtcat aattagtttc
    84841 taaggtgtct ttgtatccat cattgttaaa gtccttaata atacttttag gattctgccc
    84901 aaatattaat gtagggaaca gaaagattgt tattaaaagt gttagtttta acctatttct
    84961 taatatttcc atattcgtat agcatttttg caaataagtc ataatttgaa attttcaagt
    85021 tttctccgct tgtgcataac atctgtgtaa gtgcacttac ggtgcatatg ttttgacttt
    85081 atattattgt ttgtgttatt tatgtagctg ataattatgc tattaaattt agtatgcaat
    85141 tgaaataagg tacacctatc caatttgtta atttcaaatt tgtgtgcttt aaattcatta
    85201 tatatctaat ttatccaacg gattttttat ttgatcgaat ccttttgctg taatatgtgt
    85261 gtatatttca gtagttttac tgctcgagtg tcccaacaaa ctttgaatat atttcaaatc
    85321 agtaccatta tctaataaat gagttgcaaa acaatgccga agactgtgta ttgaaacctt
    85381 cttttttata ttggcagcag ctaaagctgt tttaaaaatt ttaaatgcta ctatcagcat
    85441 actgcccaac attttcaccc tcaaacaacc atttccgtgg tttaaattcc ttaaaatatt
    85501 cgcgtaaatt tattaaagtt tttttactta acaaggtgta acggtctttt tttccttcga
    85561 ttgtgaaact tttataatat tatgtataac gttttgccgc ttgccgcagt ggcgtatttc
    85621 ggagcactaa actgtcaata caccacaaaa gttgatgcgc ggtagaatgt tcaattcacc
    85681 acgtcatcca ccattgagcc aaacgcctgt tagcggctta cattctgtcc tccatcagtg
    85741 tcaagtcgct gtattgctat gtttttgctg tcattttgtt agtctctata tttctgtctg
    85801 tttggtttgt gtactagctg ttctaaaaat tttagaggga agggatttta aaaaaataat
    85861 ttttccttcg ggtgacaaag gtggaagctc ttttgcaatt tttggattgg gtgtgggctt
    85921 gtgcgaaaca aagttcacga acaccaaatg aatatacaac tcgtgagtaa ttgcaaatgt
    85981 gcttacacct atggattggc tttcctcttt atagtacatt tatttccttt tcaattaaac
    86041 ggtatttgtc ttttatatct tctcccgaat agaagtcaag caagtctttt tttgttaatg
    86101 ttccttccct taaaaagcgt tttaaatatt cttggttgaa ttccatcaat aagattgatt
    86161 tttccaactg ctttaaaatt tctttgttgt tgtcaatgtt tttttgaaca agtgatttta
    86221 ttgtcaatac ttcgttgatg tctttgatga ttcggtttac tattattgcc aaaatatctt
    86281 tgtcgagttc gatttctttt gcatttaaag caatgtctct cgctagcata taagcaatag
    86341 ttaaattgct gtaatcgcct ttttgtgaat tgattatagc aacaaaacca actcccggaa
    86401 tgtaacccac attttcaaaa ttattaagaa tagatttatg tactaaggaa atatcaaaaa
    86461 cgatcaaact cactatgctg tctctgttgg cttgtgcttc tatcagctga ctccaagctg
    86521 tatcagattt acgtgtgaaa atatctttac tttgaatgtc tcctaaatca acacttttgt
    86581 caaacttaca ttcaatagct attttcaaat caggcgttcc gtttacttca caaattatat
    86641 cgcctgtttt gtttttcggt aatttacctg ctgtattacc cgttaaaaac gctttgtcat
    86701 ttagtttttt gtctgcaaag tattggttta aaaattctgc aatttcattt tctgctaaac
    86761 ttcctttgat agttgattta tagaaaagtt ctttcttcat ttcaaatatc attttcaaac
    86821 tttcgagttc tgttcccaat tcattggatg tttttgaaag aaaagttgaa atacggtctt
    86881 ccataaatgc caatacacca atgtaaattg cctttagcaa tttttcacct ctttcacttt
    86941 caggaagttt gtcaaagtaa ttgaaaacga tttgattttc gatttcaaat tctttgattt
    87001 caattcgttt gagttgttgg tttattttta ttaggttttt actcaattct ttcatatgga
    87061 taaacatttt tatagtttct atggagatat gaacctaacg aaggtgcatc tcgtaaattt
    87121 tcaaattcgt gtaaaggaac atctttataa acataaagac ttccattaag gaatttcaca
    87181 taaacttgtt cgttttgttc gtcataacca atactttcaa tattggaaga actaactgga
    87241 accatttctg gtacactcat aatttaaaat ttttatttct ccaagatttt aaaaaaattt
    87301 tgtattcgtt ttcactattt tcaatttttt tgaagccatt tttctgatgc ttttgggtca
    87361 gaacttttgg gagcattaga tattttaaag aatttaattc ttgaaagtat ttttactgct
    87421 tccctcaggt cgctaagttt tgtaatccca gctctttagt tattaggatt tttaatcccg
    87481 attaataaac tgggtt
//
LOCUS       DAOTAX010000002        76568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_263_length_76568_cov_30.128749, whole genome shotgun sequence.
ACCESSION   DAOTAX010000002 DAOTAX010000000
VERSION     DAOTAX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 76568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76568
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_76568_cov_30.128749"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3149
                     /locus_tag="PLM04_00385"
     CDS             <1..3149
                     /locus_tag="PLM04_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HON01572.1"
                     /translation="KLTFLFACLFMIGVGLVNAQSLTVQGKVISDEDGQPVIGASVLV
                     KGTTTGTITGTDGSFSITVPAGNRTLVVSYIGMKSVEVPAENGMVVRLQPQTLEIDEV
                     VVTAIGIKRSEKALGYSASAVSSQDITMTGNRSALNALQGKVAGVEITSASGAPGSST
                     RVIMRGYSSLGGSNQPLYVVDGVPISNGQIGSTSINGGLDFGNRANDINPEDIESMTI
                     LKGGSATALYGSRAASGVILITTKKGEGLKNKPKVEVSSTSTFDSPLRLPYFQNEFGQ
                     GWYDRGGGEQDLMENGSWGPRFDGKIRPWGFVVDNQQQIKPYVALPTNIKDFFDIGTN
                     YNNTVSVSNGDADKSYYISYGNVTADGIMPTDADYYNRNTVSMRGSAKFLKIFNISGS
                     LNYVRKDMRFTETGQDQAALDGLWQSPRDISIVDLKDYNNKFNNVDNYYTIYAQNPYY
                     ILNEHGNKFQENRIFGNISLDAQITPSLSATFRLGDDVANSTLKTWRAITNSARADYN
                     DEVGRVGENAYYSSELNTDFFLNFNPKLNNDFAISVIVGHNFNQRDAQSQGSEVIGLD
                     IPKFYNLSNSSATPSIYGTLSQRRLIGVYGNLDVSFKDMLFLNITGRNDWSSTLPANN
                     RSFFYPSISGSFLFSELIQNKDVLSYGKLRASFARTGKDADPYLIYPVLLQTSITDGY
                     RSLDFPLAGPVNGFTISNVIGNDQLQPEISQDFEIGTNLKLFKNLLDIDFTWYNKKIT
                     ALIWTATLPSSTGYTSQTQNLGEITNKGVELSLSVTPIRRKNIEWEVFANYSKNNNLL
                     KSLISGLDEIVLGGTRGFPFVARPGYELGLFKGNVPETDPQGRIVVNAQGLPVFKAEQ
                     EIIGSSQNKYRLGGGTSLKIKNFRVHAAFDYRNGGKMYSRTAEIAYFTGNAQPTTYND
                     RQPFIIPNSVQLVGGQYIENTTPIAGFTNNLNLYYNQTYKAGIGDAYALIDKTFFKLR
                     ELSISYSFPKSVLKNAFINSVDLSLVGNNLFLWTPDSNLYTDPELTTFGNDLAADYGE
                     FGATPSTRSLGFNIKLSF"
     gene            3172..4569
                     /locus_tag="PLM04_00390"
     CDS             3172..4569
                     /locus_tag="PLM04_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HON01573.1"
                     /translation="MKRLKFYNIAAFIGLLFLSSCSESFFDINHDPNNPSEATPGLVL
                     PSALANSAYVMGGYYHALGSFWTQQYAQAPAASQWADWESYNLTNVDFDRQFTLLYAG
                     GLNDYEYVRKNTAETGNWKFYAIATLMQAYTFQVLADLYDQIPFREALKGNVIPHYDN
                     GQLVYDSLLARIDYAMSKDFTGSTVENPGSSDLIFGGNIDDWKAFANTLKLKMYLRYV
                     NVNPNTYRTEIEALLAENKFLTKDAKFTAFKEEQTGYNPFYNTFVDRLSGNVVANKTF
                     IDLLKDKNDPRMAKIFNPSVTGSLYNGIAAGDNKNHPTETSKNYATPAIKGTSPVYFF
                     SKEEVLFLIAEAQARYGTASDAESTYKTAIRASLTSLGLAADAIDIDATYPYNGIRSI
                     IEQKWIAQANKNGIEAFFDYNRTGYPNFFTRSLTSVLNGDERPKRLYFPSREVNSNPY
                     TPARVDLTEPVWWAK"
     gene            4598..5308
                     /locus_tag="PLM04_00395"
     CDS             4598..5308
                     /locus_tag="PLM04_00395"
                     /inference="COORDINATES: protein motif:HMM:NF027719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5012 domain-containing protein"
                     /protein_id="HON01574.1"
                     /translation="MKKIILMTLAFVAFVFTSCEKESLGVSRVTTYATMELNGPEELF
                     WPLNSPFVDPGCKAFEGTTDISGNIEVISNVNATKGGKYSITYKVKNSDGFYAQTSRT
                     VYVYEQNAPLNGYYQSRIRRNNNGTIANRGPFTILVFGVGNDQYWIEDLLGGWYNYGS
                     GYGPAYAGPGIIKLNSDNTFEIVSAEPLAWGYPCILTENTVSTYDPSTKTIVLNTRMK
                     DTPLMLFTVTLTNPQPLN"
     gene            5322..5789
                     /locus_tag="PLM04_00400"
     CDS             5322..5789
                     /locus_tag="PLM04_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HON01575.1"
                     /translation="MRINKFFILSILSLALFSCEPKYEKEYNWAYPVCGDWMVKAYLD
                     GTEIYGPFEIMTYNTSFGQDSIWIHDYATTATNGHFWSFKVKAAVDMKTLTFQTEGSI
                     NAIAGYPIVIKVSNGRVINKDSITMELEFEDDPGTIYQLAGHRETSYEEYMEQ"
     gene            complement(5864..6934)
                     /gene="gcvT"
                     /locus_tag="PLM04_00405"
     CDS             complement(5864..6934)
                     /gene="gcvT"
                     /locus_tag="PLM04_00405"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HON01576.1"
                     /translation="MKITPFTHFHIALGAKMTEFAGFYMPIEYTGINEEHLTVRRAAG
                     VFDVSHMGEFLVKGPHALAFLQKMTTNDVAALPVGKAQYSCFPNGRGGIVDDLLVYRL
                     DKENYLLVVNAANIEKDWNWCMQNNTENAELVNISEQVAQLAVQGPKATQILQKLTPV
                     RLSEIPYYSFAVGTFAGVEPVLLSNTGYTGAGGFELYFRPEYADTLWNAIFEAGAEEG
                     IRPIGLGARDTLRLEMGYCLYGNDIDDTTSPIEAGLGWITKFVEGKNFIDCPLLEKQK
                     REGVTRKLVRFELTERGIPRHGYPIVNNENKPVGQVTSGTMLPDSKKGIGMGYVATEY
                     AQPGTKIYIQIRNKNLEAIVRK"
     gene            7287..7418
                     /locus_tag="PLM04_00410"
     CDS             7287..7418
                     /locus_tag="PLM04_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01577.1"
                     /translation="MNIIFRHVGSIRPLKNKLYSDENGQSQSDGSMRGITGKGMSRV"
     regulatory      7685..7925
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8026..10065
                     /locus_tag="PLM04_00415"
     CDS             8026..10065
                     /locus_tag="PLM04_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON01578.1"
                     /translation="MHKNFFFYLIEIFFLFFFIPVRAEILFQDMGMDTAKTYSIPEIV
                     VVEKFQNSEIRSTMPLQILSAKKLENLNVLQLSDAIKYFAGVNVKDYGGIGGLKTVSV
                     RSLGAEHTAVSYDGIMLTDYQTGQIDLGRFSLDNVDMVSLNNGQSDNIFLPARSFASS
                     SVLNIRTLPPRFEEEKSIHGKITCKVGSFGLINPSLFIRQQMSRKISAVFSGEWLTAE
                     GEYPYLLIYGTQTGDSSSIETRKNTDVETLRLEGTIFANFFHRGSGYLKTYFYESERG
                     LPGAVIYYMNENTNHQRSKDQTFFTQAHYESSFSSLLSFQANTKYQNGYLHYVDPNYN
                     NSGGKIDNTYSQQEFYFSASFLYKAFENLSFSASTDYSLNTLNANLPEFAFPKRNTVL
                     MVLNAKYVSNQFLATAGGLQTFVNEKVKFGSSPNDKKHFSPFFSLSVKPFEQYDFRIR
                     SFYKNVFRMPTFNDLYYSYVGNRNLEPENAQQFNAGITWSFSSESISLLSVTLDAFHN
                     AIRDKIVAFPNKDIFNWTMKNCGKVSINGLDVAMESGISITRNIGLVFGGSYTYQQAL
                     NVTDPNAEDYLHQIPYTPRISGSGKAALEMPWVNLSYSFIWSGHRYTLFQNYASNRLP
                     GYADHNLSLSRSFKKSSYSCFFKLEINNFLDKNYEIVRGFPMPGRSFRSIVAIEF"
     gene            complement(10088..10693)
                     /locus_tag="PLM04_00420"
     CDS             complement(10088..10693)
                     /locus_tag="PLM04_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01579.1"
                     /translation="MKILYKIISIILQPILLPTYGILLLIQIPVFANLPMQWKAIAVG
                     GTFLFTALLPSIPVVIMYFRKEVSDLFISNREERTLPYLFSFLAYVFWTLFLVRTMHL
                     PMFIGGLGIGSSISILLITFINVKWKISAHLASLGGLAGGIFGICYRYSINPLWLFIL
                     ILLLAALSAISRVALKAHTPMQTIAGFALGFLCVFLPVLLL"
     gene            complement(10732..12204)
                     /gene="rpoN"
                     /locus_tag="PLM04_00425"
     CDS             complement(10732..12204)
                     /gene="rpoN"
                     /locus_tag="PLM04_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HON01580.1"
                     /translation="MLKQQLQQKLQQKLSPAQIQVIKMLEIPTLELEERIRQELEENP
                     VLEIGLEPEEEKKELDDFGEDYEETGNDEGESNDEIDFEEYMSDDDIPDYKLKANNTS
                     KDDKYEEIPFSAGSTFHDFLMEQLNLVPLSERDRALAEFVIGNIDEEGYLRRTPEALA
                     DDVAFQANMDTNVKEIEKIVKIIQQFDPPGVGASSLQECLLLQLEKKRNEKEIEIPKR
                     IIQDYFEEFSKKHYDKIINGLEIDGNTLKNAINEIIRLNPKPGNAWSGNILEKNMSII
                     VPDFIVENNDGELTVHLNNSNIPYLRVNAAYSEMFQDYTSNKENQTPQMKEAILFVKQ
                     KIDSARWFIDAIKQRQQTLLTTMTAIVEFQKDFFIEGDEIYLKPMILKNIADRTGYDI
                     STISRVSNNKYVQTEFGVFPLKYFFSEAMTNDAGEEISTREIKKILQECVDNEDKRHP
                     LNDEMLAEVLKQKGYLIARRTVAKYREQLNIPVARLRKEI"
     gene            12442..13506
                     /locus_tag="PLM04_00430"
     CDS             12442..13506
                     /locus_tag="PLM04_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HON01581.1"
                     /translation="MLQIFQPFRIKDITLKNRLGMSPMCQFSAQDGLANDWHLVHYGT
                     RGVGGIALIIFEATGVSPVGRISPEDLGLWKDEQIEPLKKIVDFIHQQDSKAGIQIAH
                     AGRKGSRSSPFKGEKQLTNEEGGWQTIAPSAIPFRPEEIPPHELTKSEIKKVVNQFKD
                     AALRAFQAGFDVLEIHAAHGYLIHEFLSPISNFRKDEYGGSFKGRIRLLQEIVSAVQT
                     VWPERLPLFVRLSATDWTDQGWRLEDTVELAKILYLEGVDLIDCSSGGILPNVHIPLA
                     PCYQVPFSEQIRKTGILTAAVGLITKPEEIQDILRTEKADIVLLGRELLRNPYFALHC
                     ARHVKENSKWPQQYLRAKLE"
     gene            13662..14417
                     /locus_tag="PLM04_00435"
     CDS             13662..14417
                     /locus_tag="PLM04_00435"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HON01582.1"
                     /translation="MKVNFCTLFVLFNILFYSCSPKTWQVVEATSVKIPIDSTTEKFA
                     DKEYEAALQPVKERIDAEMAVVIGYSAENMRSFAPESPLSNFCADVFRRAASDFLHQE
                     VDVSVVNLGGLRTEIPAGNITIRNIYELMPFENELVILWVRGDKLIQLFQFFASVGGE
                     GVSGISMKIKNGKATQILVGTHPIDPEKIYTVATSDYLAEGNDQMTALSEYEKISGTG
                     LKLRNVLIDFIRNETLKGHVIQSKIDGRISMEN"
     gene            14430..15278
                     /locus_tag="PLM04_00440"
     CDS             14430..15278
                     /locus_tag="PLM04_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HON01583.1"
                     /translation="MKTIQNTAILVFLLAFNSILYGGERIKLTILHTNDTHSQIEPTE
                     KSNLPVSDKGGYARRMGIIEQIRQQNKNVLLFDAGDFWQGTPYFNFYNGRIEVDGMNK
                     MKYDAVTLGNHEFDNGLDTMATVLKNAQFPILTSNYQMDSTAMATLVKPYLIIRKAGL
                     KIGVMALNVNPKGLIFTNNYKKMNYIDPVETAINISNFLKRSEKCDLIICLSHLGADT
                     LKQTLTDYDIIRKTRYIDVMIGGHSHQIITQTNLKNAAGKPVVLAQMGKSGLYLGRID
                     LELEKK"
     gene            complement(15288..16280)
                     /locus_tag="PLM04_00445"
     CDS             complement(15288..16280)
                     /locus_tag="PLM04_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HON01584.1"
                     /translation="MAVTTKMTTDKSKLWNIFKWSCLTAAFLFLGYRLICFQHYGRLL
                     SQFKEISFWKTWYLWGVILLLPANWLLESMKWQRLVRNSEKINMALAFQSVMAGFLTA
                     FFTPNRVGELAGRIAYLQPENRKAGITLSFVNSITQNIIMAICGIPAAILFFLSNKNS
                     EFAGENNFFGYFLIVCGILLLLSGIYFSLPPLVGRIKQKKLSAKWINFIACLADYSLA
                     DLLKILLLSFLRYLVFSFQFYLMLRFFGVEINGLAAAIAIPTTYLFVTFTPSMAFSEA
                     AIRSSYSVLVIGAFSGQTVQIILASTFIWVINFVIPMFVGSFVMMQTHPLRSNK"
     gene            complement(16314..16757)
                     /locus_tag="PLM04_00450"
     CDS             complement(16314..16757)
                     /locus_tag="PLM04_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HON01585.1"
                     /translation="MPLQFFRYGVSGAANMVFDWVLYFVVYNFVLRHQMLHLGFITMS
                     SHIAALALTFPVSFMTGFLLQKYVTFSASYLRGHVQLRRYLLVVIINLGLNYAGLKFF
                     VEIAHFYPTPSKMLITIITTLFSYFAQKKFTFKTASLSLNKESNH"
     gene            17022..17804
                     /gene="rsmA"
                     /locus_tag="PLM04_00455"
     CDS             17022..17804
                     /gene="rsmA"
                     /locus_tag="PLM04_00455"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HON01586.1"
                     /translation="MTLVRPKKYLGQHFLKSMDVARRIAGSLPVGSPASVLEIGPGMG
                     MLTQFLLQQPQIDLKVVEIDHESVVYLQQHFPQLQILEKDFLQLDLNQIFSHPFSIIG
                     NFPYNISSQIFFKVLDNKDLIPSVVGMLQKEVAERLASPPGNKAYGILSVLLQANYDI
                     NYLFTVDETVFYPPPKVKSAVISLKRKENPEVDYDEQLFRRVVKTAFNQRRKTLRNSL
                     KSIIPFHPMNADPLFDLRPEQLDVKTFVELTNKIGSALKEKQ"
     gene            17878..18828
                     /gene="corA"
                     /locus_tag="PLM04_00460"
     CDS             17878..18828
                     /gene="corA"
                     /locus_tag="PLM04_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HON01587.1"
                     /translation="MSELRIFYHENDGKLKISKSIDTLKKINLKDIIWIDMNDVSENI
                     ESELEQFLKIYIQEDEEIEEIEMSSRYIQTDDSIVANSNFLQDNYVMQPVSFIIKNGI
                     LVSVRNTELKSFNETVKKIFVNTSNFPSGYHVMVSLFETRVEYDADMIEDVTDQITNL
                     SKNLNTESDLDQDILLQIKELQEKVMIIRQNIVDKQRVVSNMLKCNFFPEDLIPRLNI
                     ILKDINSLFEYTRFDFDRLDYLQDTFLGLVNLQQNNIIKIFTVVSVIFLPPTLIASMY
                     GMNFKYMPELDWKFGYPFSILLMISFTVGVLVFFKRKKWL"
     gene            complement(19085..21952)
                     /gene="polA"
                     /locus_tag="PLM04_00465"
     CDS             complement(19085..21952)
                     /gene="polA"
                     /locus_tag="PLM04_00465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HON01588.1"
                     /translation="MKKLFLIDAHGLIYRSYYAFIRSPRINSKGLNTSAIFGFVNTLE
                     EVLKNEQPTHLAVVFDPEGKTFRHEAYEQYKAQREKTPEDIRKALPIIKELVAAYNIP
                     VIEVTGYEADDVIGTLAKQAEKKGFEVYLLTSDKDLGQLVSDHIFMFRPHHNGGFEVL
                     GPDQIKEKYQLENQQQVIDLLGLMGDSSDNIPGCPGIGEKTAVKLLQEFGSIDNLLQN
                     TDKLKGSLKTKIEENKDLINFSRFLATIKTDVPVALDEEALKVGPADSEKLRALFQEL
                     EFRTLLNNKFGNTFSSFPPDYPLLQTPSGNKKSSKAEQMNLFGETNRPERTQPEKEKI
                     LSKPEENIEEISPVASNGFMALKNIPHRYQLIDDKVKRSYLVSQLFLQKSVCFDTETT
                     GLDVFSADLVGLSFCFREGEAYFVTLPDNREEATKILNEFKAFFLSEQIEKIGHNMKF
                     DLLMLSNYGIDVKGKLFDTMIAHYLLQPELRHGMDYLAEIYLHYHTIHYEDLVGAKGK
                     NQLDIRNVEISKLTEYAAEDADVTFRLKQILEKELAANELEKLFYEIEMPLMKVLVIM
                     EKTGVRIDSEALRQSSEILTENMLKLEKEIYEMAGGEFNINSPVQVGEVLFDRLHLDD
                     KAKKTKTGQYSTSEDVLEKLRHKHPIVDKILEYRGLKKLLSTYIDALPQLIHPKTGKI
                     HTSYNQTNTATGRLSSSNPNLQNIPIRDELGKEIRKAFIPEENSLFLSADYSQIELRV
                     MAHLSNDQNMIEAFRNGLDIHAATAAKIYKVPLEKVTPEMRRHAKTANFGIIYGISAF
                     GLAERLGISRTEAKTLIDEYFLAYPQIKTYIESAIKKAKENGYVETLLGRKRFLPDIN
                     SQNSVVRGFAERNAVNAPIQGSAADIIKIAMVNIQRKLEKENLQAKMILQVHDELNFT
                     VPVNELETVRKLVVYEMEHAFPLSVPLIVDCGVGNNWLEAH"
     gene            22206..23180
                     /locus_tag="PLM04_00470"
     CDS             22206..23180
                     /locus_tag="PLM04_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HON01589.1"
                     /translation="MNLIEKIRKPIEKEIEQFEKSMTEELQTENPLLKNISNYLQLGK
                     GKQLRPILVILSAKLCGEVTESTIYGAISLELIHTASLIHDDVVDNTFQRRGQPSINA
                     QWNNKVAILVGDHILSKSLSSGIKADNIKILETIANVGMNLTDGELLQLDKVQHAKIS
                     EMDYFEIIQKKTALLFSSCALIGGLSVKADKNTLKHLYHFGEYAGICFQMKDDLLDYS
                     ENNEIGKPTRNDIKDGKITLPLIYALKNATNHQKHEIEQWIENSDFSDLHIEKIVQFV
                     QENGGLEYTLQKMEEYKNKAIEELNGFAEGELKQSMILFAEFMSNRDY"
     gene            complement(23185..24072)
                     /gene="deoC"
                     /locus_tag="PLM04_00475"
     CDS             complement(23185..24072)
                     /gene="deoC"
                     /locus_tag="PLM04_00475"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HON01590.1"
                     /translation="MKDRYEELLELYQCESVDDEAIKIDIDNILAEHFAENASKDVYA
                     KCLNLIDLTSLNSTDTEGEIVSMVEKVNHFRGTFPHLSNVAAICVYPALIPAVKSHLT
                     EENVGIASVAAGFPSSQTFIEVKIAETSMVVNEGASEVDVVISVGKFLEGNYGEVYEE
                     LSEIKAACRNAHLKVILETGALKNSVNIKKAAILAMSAGADFVKTSTGKIQVSATLEA
                     TYIISHAIKEWYEKTGQKIGYKPAGGISTTEEAVKHYTLVKHILGEEWLNNRLFRFGA
                     SRLANHLLSSIEGKEIKYF"
     gene            complement(24096..24422)
                     /locus_tag="PLM04_00480"
     CDS             complement(24096..24422)
                     /locus_tag="PLM04_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HON01591.1"
                     /translation="MTLQEAQQIVDNWIKTYGVRYFGELTNMAILTEEVGELARVMAR
                     TYGEQSYKAEDEKYDLADEMADVLWVLLCLANQTGVDLTDAFSKNIEKKTLRDAQRHI
                     QNKKLY"
     gene            complement(24434..25105)
                     /locus_tag="PLM04_00485"
     CDS             complement(24434..25105)
                     /locus_tag="PLM04_00485"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HON01592.1"
                     /translation="MKKSIRIHKEGWFILTGLLLLLFAVNLIIYMEFPGPAMAINTVL
                     SAIILCFFVFFFRNPKRIVEIDDPSFVIAPADGTVVVVEKTLEPEYFGDERIQVSIFM
                     SIFNVHANWYPVSGTVVKTEHHNGRHLAAYLPKSSRENERSTVVIETPGKTQILVRQI
                     AGALAQRIVTYAHPGKECHVNEHLGFIKFGSRVDLFLPLDSEIFVQVGEKTTGNETII
                     ARLNE"
     gene            25397..26740
                     /gene="purB"
                     /locus_tag="PLM04_00490"
     CDS             25397..26740
                     /gene="purB"
                     /locus_tag="PLM04_00490"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HON01593.1"
                     /translation="MNLSPLTAISPVDGRYRSKTEKYAEYFSEFALIRFRVMVEVEYF
                     ISLCEIPLKELQSVPKDCFDKLRAVYIHFQESDAEKIKEIEQTTNHDVKAVEYFLKQQ
                     FDLLGLSDYKEFIHFGLTSQDINNTAVPVSIKKALEDVYYPEMENLLKKMRELADEWK
                     DIPMLARTHGQPASPTRLGKEIMVFVYRLDKQLSLLRAVPISGKFGGATGNFNAHSVA
                     YPSIEWKNFADKFLAEKLGIQREEWTTQISNYDHLAALLDSLKRIDTILIDFCRDVWT
                     YVSMEYFKQKIKPGEVGSSAMPHKVNPIDFENAEGNLGMANAVLDFLSAKLPISRLQR
                     DLTDSTVIRNIGLPFSYILIATQSLMKGMNKLVLNESAIRRDLENNWAVVAEAIQTIL
                     RREGYPAPYETLKALTRTNEVITEQTIHDFIDSLQVSEEVKNELRKISPFSYTGI"
     gene            26778..28616
                     /locus_tag="PLM04_00495"
     CDS             26778..28616
                     /locus_tag="PLM04_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON01594.1"
                     /translation="MFKFFELLKRFIPPYKKYVFLNLLFNLLSTVFSLFSFAAIIPVL
                     QILFGLQQSNLSYQAWTWGDGWNQIISALKNNIFYYLEQMMTTAGPSMTLLYLGLFLI
                     VMTVFKTGTSYLSSYYIIPIRTGVLRDLRNQLYKKILSLPIGFFTEERKGDIMSRMTS
                     DVTEIETSIISSLEMIFKNPIIILIYLTVMFAMSWKLTFFVLILLPLSGWLIGMIGKS
                     LKQRSTLGQQQTGELLSQIEETLSGLRVIKAFNAEKKLEKRFAALNEKLRRTLNRINR
                     RYTLAHPLSELLGTVVIAILLWFGGVLILENHSSINAAEFIYYLVIFYSIINPAKDLS
                     KAGYSVQKGLASLERVDKILKAENTLPEPTESKKINSFNDRIEFKNVNFRYQSDWVLQ
                     DINLTIPKGKTVALVGQSGAGKSTLVDLLPRFYDVTEGEILIDGINIKDLNTKDIRSL
                     MGNVNQEAILFNDTFFNNIAFGVENATLEQVMEAAKIANAHDFIMASENGYQTTIGDR
                     GSKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKMVQEAIEKLMMNRTTLVI
                     AHRLSTIKRADEICVMSEGKIVERGKHDELIALNGYYKRLYDMQSF"
     gene            28884..30227
                     /locus_tag="PLM04_00500"
     CDS             28884..30227
                     /locus_tag="PLM04_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HON01595.1"
                     /translation="MSKIYDFTLSYSLFRSYVMFAFKKFFSRYIVMGKENIPETGPVI
                     FAPNHLNALMDALAVISVVPHKMPVVFLARSDYFNNKLIARFLHFCKILPAFRMREGL
                     ENVDKNNEIFEACVDVLNHNQAIGIMPEGNQGSKRKLRPLAKGIFRIAFMAQQKYGNQ
                     PGVKIVPVGLDWGNIDKYGEHIIINVGKPIEVCEYMNEYEENQPAAINQLRDRLRDGL
                     SEVTLDIATEKNYECFETATIAASAAMVEKMKLPNTEKYRFLARQKTARQLLEMEKNK
                     PETIDKLNVICKEYRENLRKLNVPSWLLDKNLKVSSLLLESLLLVITFPFFLIGLVLN
                     ALPFFSPDIIMKAFHINFPGGKGSLRFGLGMVTFPLFYILQSVLFYKKVKDSLWAVAV
                     FLPLQYYLGKCAFLWHKKFRKLKVKFRYHKLQVKNSLILQRTKLLHEEILQLVQS"
     gene            complement(30330..31148)
                     /gene="tsf"
                     /locus_tag="PLM04_00505"
     CDS             complement(30330..31148)
                     /gene="tsf"
                     /locus_tag="PLM04_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HON01596.1"
                     /translation="MAVTLQEITKLRTMTGAGLMDCKNALIEANNDLEKAIEIIRKKG
                     QAIAAKRSGREASEGCVLAAAKDGFAAVLALKCETDFVAKNADFMALTQSILDVAMDK
                     KPATLDELKAFTINGRTIADLVVDESAITGEKVELDDYNFVKGGTTAAYIHQGNKLAT
                     IVAFKEENVDHQVARDIAMQIAAMNPIAIDRASVPAHIIETELEIGREKAREEGKPEN
                     IIDKIAEGRLNKFFQESVLLEQTSVKDSKITIGEYLKAHHATIIDFKRVSLNLD"
     gene            complement(31368..32204)
                     /gene="rpsB"
                     /locus_tag="PLM04_00510"
     CDS             complement(31368..32204)
                     /gene="rpsB"
                     /locus_tag="PLM04_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HON01597.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTAAKIDEAAQAMKQIAKSGRKILFVATKKQAKDLVAEKAKAVGMPYITERWPGGMLT
                     NFPTIRKAIKKMSTIDKMMTDGTFDNISKREKLQITREREKLEKNLGSITDLTRLPAA
                     IFVVDVMKEHIAVKEAEKLGIPIFGIVDTNSDPSNIDFVIPANDDATKSIEVILNALI
                     DAIQEGIEERKLEKVETEAAEAPVVKERKSRVNKRPRTQREDDEALKANVVNKFIKEE
                     EN"
     gene            complement(32373..32759)
                     /gene="rpsI"
                     /locus_tag="PLM04_00515"
     CDS             complement(32373..32759)
                     /gene="rpsI"
                     /locus_tag="PLM04_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HON01598.1"
                     /translation="MEIVNALGRRKAAVARVFVNEGTGKIIINKRELSVYFPSLLLQY
                     IVKQPLDKLNVTEKYDIKVNLKGGGFKGQAEALRLAIARALVKINPEDKPALKSEGFL
                     TRDPRIVERKKPGRPKARKRFQFSKR"
     gene            complement(32765..33220)
                     /gene="rplM"
                     /locus_tag="PLM04_00520"
     CDS             complement(32765..33220)
                     /gene="rplM"
                     /locus_tag="PLM04_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HON01599.1"
                     /translation="MDTLSYKTISANKNTVQKEWVLIDASDEILGRMGTKVAKLLRGK
                     YKPNFTPHVDCGDNVIIINASKIKLTGNKWTDRIYLRHTGYPGGQRETTPAQMLAKDP
                     ERLIRKVIKGMLPKNRLGAKLLKNLYVFPNAEHNMQAQQPKQIDLNSLK"
     gene            33574..34455
                     /gene="era"
                     /locus_tag="PLM04_00525"
     CDS             33574..34455
                     /gene="era"
                     /locus_tag="PLM04_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HON01600.1"
                     /translation="MHKSGFINLVGNPNVGKSTLMNALVGEKLSIITSKSQTTRHRIL
                     GIVNEENYQMIFSDTPGILKPKYALQESMLQFSRSALTDADILMYVTDVYDAEPKDMD
                     FVQKINSDNVPLLVLINKIDLINQEKLELLVEKWHGIFPRGEILPISALEKFNVDYLM
                     KRIKELLPESPPFFDKDQLTDKPVRFFISEIIREKVLLNYQKEVPYAVEVEIEEFHEE
                     ENLVRIGAVIYVERDSQKGIIIGKGGKSLKKVGVQARKDIEEFLGQKVFLSLYVKVEK
                     DWRNKEKKLKDFGYLHD"
     gene            34561..35190
                     /gene="pdxH"
                     /locus_tag="PLM04_00530"
     CDS             34561..35190
                     /gene="pdxH"
                     /locus_tag="PLM04_00530"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HON01601.1"
                     /translation="MLKDLRKQYDRSTLNESNILKNPFQQFKLWLSEAINTEESEPNA
                     MVLSTVDAEWQPHGRMVLLKELTDEGFVFFTNYKGNKGLQILHNQRVSLLFFWPVLER
                     QVRITGNAEKIAEQASDEYFKMRPLFSQIGAWASPQSRKIPSRDFLDKQFEYYKHKFE
                     TEVPRPPFWGVFLVRPKTFEFWQGRPDRMHDRLFYTKNDQQWEISRLAP"
     gene            35580..36800
                     /locus_tag="PLM04_00535"
     CDS             35580..36800
                     /locus_tag="PLM04_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HON01602.1"
                     /translation="MKKILLSVMLLAVAVTYASAQSTAETKAKADIAHWSAGIKAGVD
                     YYRVTPSSTVTDYLFFENSWARYANNAAWTAPQIYVEYTFNPLFGLGADFSYLTYGRN
                     TGAGHTLDFTLYGSTNLSNLLAPKRAGFWGKVNIYGNLGAGLGFYSYSGVAGVTDGDN
                     LKSPLAFAALNAEYRLSKVLSLGVEGQYRYYMRENLGGLSNNSLGSDALIGTIGLRYK
                     FGGATKSHVRDMSLEEYYPAPAPVVIEKTVQDGKVDANTENRLKALENDNAMIKNKLQ
                     QLESDLKALSQKSTGTVNATFQNIEFEFGSSQLTKQAYDLLDQIYAILNSNSTAWTKI
                     IVSGHTDNIGPKSVNQQLSIARAQAVKDYLVKKGIPASAITVAGYGEDKPIASNDTPA
                     GRQKNRRVEFEISK"
     gene            complement(36882..37718)
                     /gene="pyrF"
                     /locus_tag="PLM04_00540"
     CDS             complement(36882..37718)
                     /gene="pyrF"
                     /locus_tag="PLM04_00540"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HON01603.1"
                     /translation="MNRKELFENIKRKKSFLCIGLDTDINKIPIYLKEKSSNPIFDFN
                     KVIVDATADLCVAYKPNLAFYESLGIEGLKALNETVAYIRNHHPDQFLIADAKRGDIG
                     NTSAMYAQAFFEKMNFDAITVSPYMGEDSIKPFLSYKNKWTIILALTSNKGAFDFQLI
                     KEENTRERLFEKVLKTSTNWGTEENIMYVVGATKTEYLKSVREIVPEHFLLIPGIGAQ
                     GGSLEETAKFGLTSQCGLLVNSSRQIIYASSDENFAEAARFEALQLQQAMENILLKSG
                     II"
     gene            complement(37748..38833)
                     /gene="prfA"
                     /locus_tag="PLM04_00545"
     CDS             complement(37748..38833)
                     /gene="prfA"
                     /locus_tag="PLM04_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HON01604.1"
                     /translation="MAEKTLLEKLEGLQNKFDEISTLITDPNVISDQKRFAKLNKEYH
                     DLREILNVKNEYELALSHLTEAKQMVETESDTEMRELARSEIEELEQKIPELENKLKF
                     LLIPADPEDRKNAIVEIRGGTGGDEAAIFAGDLFHMYAKFCESKGWKIEVTDYNEGTA
                     GGYKEIIFTVSGENVYGTLKYESGVHRVQRVPQTETQGRIHTSAATVAVFPEAEEFDV
                     DLKESDIRVDIYCSSGPGGQSVNTTYSAVRLIHIPTGITVQCQDEKSQHKNKAKAMIE
                     LRSRIYAIEHQKYLDEIGSRRKTMVSTGDRSAKIRTYNYPQGRVTDHRINYTIYNLPA
                     FMNGEIQTMIDQLMIAENAERLKDAEL"
     gene            complement(38960..40384)
                     /locus_tag="PLM04_00550"
     CDS             complement(38960..40384)
                     /locus_tag="PLM04_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HON01605.1"
                     /translation="MAFLNNTLIVRRDLMTKLVSLFNEGVLLSEIDRVPLKMSPKKAQ
                     ARGRCCIHKERAVLKYKIMPILGYSIEEEEDELTPLSEYARKALLRTEKNKSIFTVVD
                     EACTACVKTNYVVTNLCKGCVARACYMNCPREAVFFKENGQAGIDHNKCINCGICKEA
                     CPYHSIVFVPVPCEESCPVKAIQKDENGIEHIDESKCIFCGKCINACPFGSIFEISQI
                     FDVLSAIQRNEKITAIVAPSVQSQFEHSFSDIATAIKNIGFSEIIEVALGAMETAFHE
                     GEELQQKLEQGQQFMTTSCCPSYVELTKKHLIEMKPFVSQTNSPMYYASKIAKNKYPD
                     SKVCFIGPCVGKRKEAADDQTIDYVLTFEELDAILKGMKIEIIPTENKDDAEIKAGRG
                     FAQSGGVIAAVMESYPNVGIKPLIVTNIDKKNIALLKAYAKGKAPGNFIEVMACEGGC
                     ISGPCTINTYKDAQKIFKKVVEEN"
     gene            40792..41946
                     /locus_tag="PLM04_00555"
     CDS             40792..41946
                     /locus_tag="PLM04_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HON01606.1"
                     /translation="MFSFNKIYRLSQRLRLVFIGSAALIVTASLFFTNRLTRQLATEE
                     HKKMEIWAGAMRQVILAGENTDINFLSEIISGNTTIPVIMTDENDNLISSLNVNEPAS
                     KTNDYYRREIERLKKKNSHIEVRIDNTLQHIYYDDSLLLKQLRYFPYIQLGVIFIFLL
                     IAYLAFSGTKKAEQNQVWVGLSKETAHQLGTPISSLLAWLDLLRIRNDDKLLTEMEKD
                     VNRLKIIAERFSKIGSAPDLQLVKLNETITNAVQYISHRSSQKVIITCHFQTSDNDLI
                     RMNVPLFEWVIENLCKNAIDAMNGNGKIDIFVQKKSNEYIVDVKDTGKGIEKNKFKAV
                     FSTGYTTKPRGWGLGLSLAKRIIEEYHKGKIFVKQSEIDKGTTFRILMKA"
     gene            42070..42639
                     /locus_tag="PLM04_00560"
     CDS             42070..42639
                     /locus_tag="PLM04_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HON01607.1"
                     /translation="MSKFELYNIVLKDLTNEKRIVEFDLDDAYFQKIDSPEVQQGNVH
                     AKITVQKKTTVYKLDFELNGFITLPCSRCLEDMQQLIHYKESLEVKFGNKFSEENDIV
                     IVPEADGAINVAWFLYEFIVLNIPLKHVHAPGECNKAMITKLKKHISSTKEYEDETSL
                     ELEDTNEDDYFSNNQFIDLEFDNLQNIDN"
     gene            42665..42850
                     /gene="rpmF"
                     /locus_tag="PLM04_00565"
     CDS             42665..42850
                     /gene="rpmF"
                     /locus_tag="PLM04_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HON01608.1"
                     /translation="MAHPKRKHSNQRTAKRRTHDKAKLPTLAICSNCGATYIYHTVCG
                     ECGYYRGKLAIEKLANA"
     gene            42961..43959
                     /locus_tag="PLM04_00570"
     CDS             42961..43959
                     /locus_tag="PLM04_00570"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HON01609.1"
                     /translation="MSKINAVITGVGGYVPDYVLTNEELSTMMDTNDEWITTRVGIKE
                     RRILKDKELGTSFMASKAVEDLLSKSGTNPDEIDMVLCATTTPDYIFPACAAIVTQNV
                     GIKNAVAFDIQAACSGFIVGLTTATGLIESGRFKKILVIGAEKMSSITNYEDRTTAPL
                     FGDAAGAVLVEPTTENVGIIDSILFTDGVGISHLNLKGGGSVYPASHETVDKRMHYIY
                     QEGQFVFKHAVSDMSDATVKILERNHLTKDDIAWLIPHQANLRIIDAVAQRIGLEYEK
                     VIINISRYGNTSAATIPLCMWESEKKFKKGDNIILTAFGAGFTWGAVYLKWGYTKE"
     gene            44532..46250
                     /locus_tag="PLM04_00575"
     CDS             44532..46250
                     /locus_tag="PLM04_00575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HON01610.1"
                     /translation="MKNYVVSARKYRPTTFKSVVGQTALTNTLKNAIKSNHLAHAYLF
                     CGTRGVGKTTCARIFAKTINCEHLTEDFEACNECESCKAFNEQRSYNIHELDAASNNS
                     VEGIRSLIDQVRIPPQIGKYSVYIIDEVHMLSSSAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFNRISVSDIVGHLQYVAHEEGITVDVNGLNVIAQKCDGGMRD
                     ALSIFDQLVSLCGNNISYQDVLTNLNVLDYDYYFQLIEAFMKNDVNTSLLIFNDIIQH
                     GFDAHHFVSGLSSHFRDLLVSKDPQTIQLLEVGADIGKHYQMQAQQCPEDFIFKALKI
                     CNDCDLNYNISKNKRLLVEIMLIRLCQLVKQEDKLNGEEKNEPLKKVVDELKTNNVVL
                     AKNSPSSTKVQDSTSNPMSTDVDAKQVKTERPTSISIKNNVVSSPSSSYEAPQKEQPD
                     IRNEIFTQDDLQKAWAELAKTLVDEARMTAFMQSNRPELISNTEFRILVTNELQEKEL
                     KRLKPTILNFLKTALKNSNIKMSIQIQEDRSVQKGRTPEEKYKIMAEENPALAKLRND
                     LQLEID"
     gene            complement(46591..49323)
                     /locus_tag="PLM04_00580"
     CDS             complement(46591..49323)
                     /locus_tag="PLM04_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HON01611.1"
                     /translation="MKKILFTFSLLLCYLLSWGQIITTNPTFITKDYPGEIEIIYDAT
                     QGTAGLKDYTGDVYAHTGVITSSSTSPSDWKHAPVWGDNSPKYKLTSLGNNKWKLLIT
                     PNMAGYYNLSPGEVVRKLAFVFRNSTGTLQGKDIGGADIFVTVYESNFNVAFTFPTSN
                     QSVAIGSSIDFTVSSSMSAELKLLLNNTVLATQQAATSLTYSYNFSVAGDYTFIASAK
                     VDNNTVYDTLLVCVPEPVVNQTRPAGTKLGINYIDNTTVTLVFHAPNKTNVFLIGEFN
                     DWAQLNAYQLKKDGEYWWITLTGLTPGKIYAYQYLVDGTIRCSDPYTELVLDPWNDKW
                     INQYYTIYPNLKPYPEGKTEGLVATFQTAKPAYNWEITNFSMPAKENLVIYEMLLRDF
                     TTEKSLQAAIGKLDYLKNLGITAVELMPIQEFDGNNSWGYNPNHYFAPDKAYGTPEMY
                     KKFIDECHKRGMAVILDMVFNQASGICPFALLYWDSTKNRPAADNPWMNPVAPHQFSV
                     LNDFNHSYSGTKEYFKRVLQYWITEYKVDGYRMDLTKGFTQNSGTESTWDQTRINNLK
                     DYYYAVTDVKPDALFILEHFVGGSEEDYLAAQGMYLWRNLNNAYSQAAMGFQSNSDFS
                     GMNTSPRRWVGYAESHDEERNFYKAKTYGNGTVKTDSLYRISRVPLVVAFATLMPGPK
                     MLWQFEEMGYDYSIDSFGGRTNPKPHAWGWLNLPHRKAAADACAKIISMRKLYPNAFL
                     NGNYSLQVAYNDWSQGRRIALSHADLNMVVLGNFQSDASITANPSFQRTGTWYNLLTG
                     ETLNVSNTNMTINMQPGQLLIFTDRNVNFTNDNREISYNVSDYVYPTLTSGKLFISST
                     ANIQSIRIYNLQGSLLKQQNNDIKEIDLSMLKKGFYLVELETANGKSAHKIIKE"
     gene            complement(49554..49721)
                     /locus_tag="PLM04_00585"
     CDS             complement(49554..49721)
                     /locus_tag="PLM04_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01612.1"
                     /translation="MVEWITDDATPYNNLTKNVKSHHAQVIKKDDMPKILPWLYIAIG
                     NVKRLLLDVHH"
     gene            49990..51708
                     /locus_tag="PLM04_00590"
     CDS             49990..51708
                     /locus_tag="PLM04_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HON01613.1"
                     /translation="MKFSFLSSLSLIIVSANICLSGFAQCGKGEYTISAFPCLNCAVT
                     CQKPAGPEWIHNATFYQIYPQSFKDTNGDGIGDLKGVISQLDYVKSLGVDAIWINPFY
                     VSPFRDAGYDVADYYHVDPRYGTDQDAKDLFRIAKQKGLRIIVDFVPGHTSIDHPWFK
                     ASCSSTPTKYSNWYIWTNGTWFDDENGKYKANFIQGFCDRDGNYMINFFWHQPALNYG
                     YGQPDPKQPWQLSTTHPDVVALRTEMKNVMRYWLEMGCDGFRVDMAGSLVKNDPEGSI
                     KYFWQDIRKMLDQEYPDAFMVSEWSESATAVNAGFHADFMLWQEGYQDLFMHENSYFR
                     SAGIGSISHFMWRYNEQSTETNGKGYISLTVGNHDLERINNRGRNRADLEIIQVFNFT
                     MPNIPFIYYGDEITMRQLPLVTGKEGCYGTRAGARTPMQWDTTANLGFSTSAPERLYL
                     PVDSSRNAPNVLAQENDPDSFLNFMRKLVLLHKTEPALTNYAKFVPVYVQKNKYPFIF
                     MRIFGNEKLLVILNPDSKPATAIVEGNLKGKRKLLLGKLPVWKQNTGTTEIQVDGQSY
                     SVIKLK"
     gene            51721..53157
                     /locus_tag="PLM04_00595"
     CDS             51721..53157
                     /locus_tag="PLM04_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HON01614.1"
                     /translation="MKKVSRYYFIFSALALLLLFSGCRSSQKNSKTSKTGFTPEAYTA
                     LYADKWSAINDSVKAGWKNHSGKPSNNAVPDTFAYAFNEWTLFYWDLYFTQIGLLKHN
                     ELKLAKGGINNLKAELDKRGFIPNANTDWGDNRSQPPYFGIMVKEFYDFTKDKKWLKT
                     MYAAVLKEYKFWTDTSANRIEANTTELGLQRYFHHATETELLELYDQELTSRFDFPKD
                     IEKSKKLNIASQFAAEAESGMDFTPRFENRCSEFIAVDLNSNLYLYEKYLAYFEQELK
                     IGNGQQWLAKARKRKALIQKYCWNDERGLFLDYDFVNNRSSKVAAATAFSPLFAGLAS
                     KYQADRMRENLHLFESENGIVACESTDEKRIYQWDHISVWSPMQSLVIMALDNYGFKE
                     DACRIAMKHLDLITANFFNPNPSTYVDKSGKKFYRLPGGIYEKYTRFGTINDREYNAS
                     LMYGWSAGAFAFAYDYLIVNSQHNFTIK"
     gene            53154..54548
                     /locus_tag="PLM04_00600"
     CDS             53154..54548
                     /locus_tag="PLM04_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HON01615.1"
                     /translation="MKKIRILFIITFLFISLFGQKILCQSELKITSDHHYLSYKNGDP
                     FFWLGDTAWELFHRLKLEEIETYLENRKQKGFNVVQVVVLPEMDGLRVPNRYGDVPLI
                     DMDPTKPNEKYFQFVDTVVKLAAKKGIFMGLLPTWGDKVTQLWGEGPVVFNPENAYKY
                     GKWIGERYKSYKNIIWILGGDRPPMKDSADWRPVWRNMAKGIEKATNHSALISYHIWG
                     GEMSTSQYIHSEKWLDINMMQSGHGSGKDVPVWEWIKRDYCMKPTKPTLDSEPNYEDH
                     PVNPWPSWNPENGYFRDYDVRKQTYRSVFAGACGVTYGNHSIWQFRNERDPNINFAER
                     YWTEALDRPGAFQVGYLKKLIESRPSSSRIPDQSIILEGQGEKEEYICAFRNRSNDYA
                     MIYIPVGKTITIKTSFIKTKKLKISWFNPQTCETIQSIETINTGKMCVTTPTTGFGKD
                     WVLIVEKDLVNTAK"
     gene            55051..56352
                     /locus_tag="PLM04_00605"
     CDS             55051..56352
                     /locus_tag="PLM04_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HON01616.1"
                     /translation="MLTAQNPICPPGLNIADPSARVWNDGRLYVYGSRDESFNYYCSF
                     DYWVLSTDDLIHWQYTPNVFASKGQYDQVPFNDNLLFAPDCMYKNGTYYLYYCQPDGI
                     QGVATSLSPIGPFTNAKEIYLKNKNQIDPAVFVDDDGQAYYMWGQFQAKIAKLKPNMT
                     EIDTTTIVDNLVTEKEHFFHEGGYMVKRNGIYYFVYAHVGRKNMPTCIGYSTSKSPLG
                     PFKYGGVIIDNDGCDPANWNNHGSIVEFKGQWYVFYHRTTNGCVNSRKACIEPIYFNT
                     DGSITEVEMTSQGAGKPLDAFSKIQAEHACLLSGHVRVESYSFDTNNPLNPTNSDQLG
                     QIEDKDRAMYKYIDFGEGVDSVSIRIAPGLNSGKISFILDNSWSLPFAEIAVDGGGDG
                     KTWKIFSCKVKRVNGIHALNLTFSSTGSNTFNIDWFTFKHQ"
     gene            56500..58143
                     /locus_tag="PLM04_00610"
     CDS             56500..58143
                     /locus_tag="PLM04_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01617.1"
                     /translation="MKKLVLLAFIFSTIFAFGQLPTFKISKKVIADIDDKLFGQFMEK
                     PSWDGEIGGDGAIDKKTGSVSPDVLAYLKEMKIPIIRYPGGTDVDYYQWYNLIDNVPG
                     KHQKRPAYRNYSGTGKITSDNRLGLDAFLTLCSELKSEPLLVVNIGDAFFRKISIEES
                     AKNAAGFVAYCNSKVGTKVSEGMPDWPAIRAKNGHTKPYNVRYFEIGNEIQLFKSMKE
                     KDVNDSLVNHYVNCLEAIVNAMLDVDPNIKIITDGEIKEVSEQIKRRLGNKISYLACH
                     YYLPWEINTVKLDTTTIDIKSLTQADIWNAWVATPYIHPETGQSYFPKTGKYNHAADA
                     GYDLAITEWNWNGWWNKKILPDAALNSLFAKGIGAAGFIHAMMRDAGRMKIGCQSMLV
                     GNVWDINAIRVDTTGKMKARFFPTGKVTTLYSKYHGNQLLEMSEKNQLFYTQPYKMNS
                     IEAVPRVTYVDALASRNETTLYISFINRNFANDMDVEIDLSDFKTDSKYKQHIFTTND
                     MITNELGYFEDKTEKVKNHKPLIVHLPKHSLSIFEIKIF"
     gene            complement(58670..60004)
                     /locus_tag="PLM04_00615"
     CDS             complement(58670..60004)
                     /locus_tag="PLM04_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HON01618.1"
                     /translation="MRFLFITLLFSNFVLSSICQQNHTLHPDKRWFKDAKYGVFVHWT
                     LNVSHKDRERMPYEEFQIKCFDEAKKFTANNYDPKLWAKMFKSWGAKYVVLTTKHHLG
                     FALYDSEYNQFTALKSTPANRDLVREFCDAMRYEGIKVGLYFSLPDKFNYDYPTLVTQ
                     NNGKEVIWQQSDFVAWKRFTDNMLNEIKHLCTNYGRIDLFWFDGDWERNADMWRSKEI
                     VDTINKYQPWAVLNNRLRSANIGDFSTPEMVIPIDKRNGMWELCTTLGYNWDGPESQK
                     SIKKPSETVRILGDVITQGGNLLLNISPDYSGSISQPQIKSMELLGKWITEHAEAIYD
                     TEQGLPLGLFNGGSTRRGSTIYLIAYETAPELVLKNIEGEIESIVHLKSGKHLTWRNM
                     DDYHSDNNRKGWRFISLPTNLIEPYATVLKITFKDKKVKVKKHDGTYLEWKE"
     gene            complement(60022..61533)
                     /locus_tag="PLM04_00620"
     CDS             complement(60022..61533)
                     /locus_tag="PLM04_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HON01619.1"
                     /translation="MSKFSWFIVVFLFLSSCKSLSGYYDVTDFGAKNDSTKLSTKAIQ
                     KAIDKANANGGGKVVFPQGVYLTGTIVLKDNVTLYFNEGSRLKGSDKHADYLFHKTKI
                     SVYPNPNRAYLIYADGVKNISIEGKGVIDGNGTKFWEPFDTLPRWIKPLNGRVSNMIE
                     INNSKNIVIKGVTLSNSPEWTCHLFDCDDVLVEGIRLVNNLYGPNNDGIDISGCNNVM
                     IANSYIETCDDAVCIKTFPSSRESSNITVTNCIMKTTCVALKLGENFKRIADVTFSNC
                     IIYESSRAIGIYGDCGGTTENINISNIVCNTNAPLILNRPIQIGIWKGKGNPGKIRNI
                     SISNFTATTEGRIMLTAIEGSLIENITLRDVNLVYPMIEDPSKYAEGTTSNQFSSIDI
                     DAKKAKAAIVAQNVNGLVIHNLNITWPHDTIPTQWKFVERIENGSNRVHRPDYATPKA
                     TEFSIVWAKNVDSGYIYAPLAKPSAHNVKDYLLINSNIKIKQTDTIRYDEKPL"
     gene            complement(61582..63765)
                     /locus_tag="PLM04_00625"
     CDS             complement(61582..63765)
                     /locus_tag="PLM04_00625"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HON01620.1"
                     /translation="MKKLLFILILIPILLPAQKNDEFLHALYDKLHDSPMQQKIREIA
                     PMPFGVVFLPWAGCTEQDMRQHFRMMKRLGFHNLKQTMATPEWSEKEILRIALEEDVI
                     PFWYGEGGWEPITTDLLRKLNIPLSATKEEIRKNPKMLEYQKNVFQKQLADWKKPISD
                     TTLIPYRHEPDAYLRPSDINLFKQWVKNKYQSIDQLVTAWNQYEVGIKKIPYRSWNDF
                     ENDKFLFSAPSNDEIIPSSGREYGFVKDVLRFKADMYLKNVKNFVVSNHPEQPVRAGG
                     EMGLFLPFASRAIDMEGIADVMKDRGSFYPSIHLAWHFEEVDYEVTRCVYMQSSLATD
                     WFKGGWAAVWESTGGPQQFSGGKGWDRKGAESTAGFTVDGGTITQMLLSYLAGGLKGV
                     GLWAWNYRRAGWESGEYALLNRQNEPGERAIRAGQIAQAAEKYRDELWAAHKEPYVGV
                     FVNWDNEAIWAACAVPNRTHFKNYPVQARIGISRALINANIPWEHVTPSDLKAGLAMR
                     YKVIYLPAQVAINEDLFPIFEQYVRDGGRLVLDAPGAWWNEQGKVLDTGKGSAFERIF
                     GASIADYQYANNVKPQLANLNLTGFLLDIKPTSADVLQKFNNNAAAVTEHRLDKGSAI
                     ILSPDASFSMKNKGNEPMENWVINVIMQDIRKPYECKNAIVYRLGSEMADHYFILNDG
                     SAKNVSLKLNRYKYRHAIDAVTQQKVSISSINIEANGGRWIRCEK"
     gene            complement(63808..65325)
                     /locus_tag="PLM04_00630"
     CDS             complement(63808..65325)
                     /locus_tag="PLM04_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HON01621.1"
                     /translation="MRKKFVLFATVVTLFSTTPTAYPNIYKPTQLGAIPDGKTLNTAV
                     IQQAIDKANSHGGGTVLLDSGVFVSGTIFLKSNVTLHIAAGATLLGSPRIEDYTELTW
                     GHNVDRQPYHLIVADNAQNITIEGKGTIDGNGEYFWEEYERNADGNMVIPRWIKAKKL
                     KVSPLIEITRSQNIYIHDVTIKTGGGWNLHLFDCDVAKIHGVNIINNLYSPNSDGIDI
                     TGCNDIIISDCYIKTCDDAICLKTTPDSRSCHRVTVTNCVIETLCVALKLGCTESSKD
                     ITDVTFNNCVVNKSSRAVMIGVRDGATYDRIAIINIVANTNAPLVFNRPIQILVNKLN
                     PDSKAGTVRNVLISNFICETEGRILLTSAEGGLIENVILRDIVLRYPMIESPQRYIGA
                     KVSSQFPKPTDHPEAVTANAALVAENIKNLVIENMIVDWPLTDSTPINWQHPERIENG
                     TSHIHKPDYTKARQTEFSAIWARNVQGGYFRTPLLKSSSKTKPVFDIKKSNIKIL"
     gene            complement(65366..67672)
                     /locus_tag="PLM04_00635"
     CDS             complement(65366..67672)
                     /locus_tag="PLM04_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HON01622.1"
                     /translation="MNKTFLLICLFLFQLFNYSPLSAKTKFPAYKDPSQPIDNRVKDL
                     LKRMTLEEKIGQMSQISSSELNDSKPGDPKSRRFRPYLDPEKAKKLIKDYHIGSFLVG
                     FAVEPEQWYKFTYELQSIAIQNSRLGIPMIYGNDHIHGANYVIGATIFPQALGLGCTF
                     NKSLAAKMGEVTALEIADLGQPWNFAPVLDLARNPYWPRMYESFGESTHLISEMGTSY
                     IKAMQTAQAALPYKIAATAKHFIGYGESKTGWDRTPAVISDQELYEYHVPPFREAIKA
                     GVKTFMINSSEVNGVPVHASQRLINGLLRKELGFDGVVVTDWADILQLIGQHKVARDE
                     REATKMALLAGIDMSMIATQTTFCDVVKDLVLSKEVPMSVIDKSVSRILKLKFELGLF
                     ENPYPRNDRFKRIGLPENNLAALQAATESMVLLQNDGVLPLKDIKNILAVGPTAHSKR
                     NICGAWTLEWGGAPEEMFPADMETVFTALLKKYPSAVVDTISNPLINLSAFQNKAQIS
                     DIIIVAVGEEPHAEGRGNIEDLTLPVDQLEIIKIVQATGRKHILSIFSGRPRVITSVA
                     EKANAIIWAGLPGYEGATALANILNGDANPSGKLCFTYPRSVGHITPYNVKVHDTYTY
                     KWPFGHGLSYSKYEYGNLILSDSIVKPGQTLKISVQVTNKSEVGGSEAVLWYLSDVVR
                     SISPARTNLVGFDKIYLEPGESKTVNFDITPEKQLTFPNEKGEMLLEDGEFVIKVGDK
                     KQSFYFTKSGHTTPKSGKEGRSHLQEEI"
     gene            complement(67729..69300)
                     /locus_tag="PLM04_00640"
     CDS             complement(67729..69300)
                     /locus_tag="PLM04_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON01623.1"
                     /translation="MPKLHTISFIVTLFLVSTVFGKKTTSVDICIYGANSGGVIAAYT
                     AQKSGKSVLLIEPSSNLGGLSTDGLGYTDIGNKYVVKGLSRDFYRRLGTHYGKLEQWI
                     FEPSVADGIFNDYVKEADLNVLYNMRLNKVNKSKNTIQSIVIENSIKPSKATNQTISA
                     KIFIDCSYEGDLMARAGASYTIGRESNTLYNETYNGVQLLDAHQFPDGIDPYKEKGNP
                     TSGLLWGISPNPVLPNGTGDKKVQAYNYRICLTDKPSNKVEITKPDNYQPEKYELLIR
                     LKEKQPWNSLNDIFIWSMMPNRKTDINNRGGFSTDMIGMNWNYADASYDERKRIIKEH
                     EDYTKGLLYFVGHDERIPLKIRNEMLKWGYPKDEYIGNSNWSPQLYVREARRLVGEKV
                     MTQHHCQGKEIVSDPIAWAAYTMDSHNCDRHVVNGMVKNEGNVEVGGFPPYPISYQSL
                     VPKRKEASNLIVPVCMSASHIAYGSIRMEPVFMVMSQSAAIEACQAIDAKVGVQDVDA
                     NKVLSEQPMLYYLSL"
     gene            complement(69304..70842)
                     /locus_tag="PLM04_00645"
     CDS             complement(69304..70842)
                     /locus_tag="PLM04_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HON01624.1"
                     /translation="MKRNFALLFILSIFIFSANAIEINVLNEGVVSDSNLVQTAKIQQ
                     LIDKCDKAGGGKIIFPAGAYKTGSLVLKSNITFRVETGATIIASRDTNDYKAVQNNSG
                     DIIPCLLYAKDAQNISIEGNGVIDGKATHYWGDLDMVDGFIKDITENAQKAGVPMKRY
                     YHIKPYTCMIYFENCKFVSIRDITLNASQFWTLHARWTDYMTISGVKVFSDLEKGINA
                     DGIDIDGCRNVTISDCIVETGDDAIVLKTTNLHGESRSCENVTVNNCVLQSSSTALKL
                     GTESHADFRYISFNNCVIRNSNRGLSIVIRDGGTAENIQFSNIMVECSRRHFNWWGNA
                     DPIWLVVLKRRPDSRVGSIRNITFNNIIAHGMGTSKIEGFEDKPLENIRMNNVQFFMH
                     PENYIDKRADDAFRAVNVNDLWLTDVSVKWDTTSVEPLWRNAFTFEKVNVLVLDKLSG
                     EEAPTAKGSFIELRNTDNVLIENCITNKESNVFLKSVSKTNKRVSVGKNIGIGKSKLT
                     ILKK"
     gene            complement(70973..72514)
                     /locus_tag="PLM04_00650"
     CDS             complement(70973..72514)
                     /locus_tag="PLM04_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HON01625.1"
                     /translation="MRKFFYVLIACLLVADLSFSKELFYITKFGAKADSKTNNTKAIQ
                     KAIDAAAVKGGIVIVPAGEFLSGTIFLKSNTELHLMQGAKLIASPRIEDYAHLSWGHH
                     EDRTPWHLIVAKDQKNVTISGKGEIYGNGPAYRDPNRKNEFSFFREIEYRPSPLIEIQ
                     GCQQVQIKDILITESPGWTIHLYDTDNAKVRGITIENSLYGPNSDGIDITGSHEVIIS
                     DCNIKSGDDAIAVKTTEDSRSCERIVVTNCVFETNCVAVRIGFESRKDISDITVSNCV
                     IKNTSRAIDVRTVEGGNIRNVFVSGITGKIDSGWAVDRIIEVHSNRIDNPYDIAIKEH
                     PNFGKAKPVEKAGVIENIHFSNMHFRTSGRILVSAISEGLIDNISFENINLTYNLLED
                     AREIGKVAQSVGFARNLPFVRSQNATFVVENAANVRIQNYTVKWPVYPVPDDCLLLTS
                     PNRLYNKWFFDGNEAKIKSGEMRVKYDVFAFRNAQNIQLQNTTSVNSENGAEYFSSEN
                     SIILK"
     gene            complement(72517..75135)
                     /locus_tag="PLM04_00655"
     CDS             complement(72517..75135)
                     /locus_tag="PLM04_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HON01626.1"
                     /translation="MLNVNAAYACQTINEGWQFVKTPGNKTVIPTFENAEKVHLPHTW
                     NMADVWDDVKGYYRDAAWYRKSILVADSLKNKKIFLFFEGANQEAAVYVNGQKAGEHK
                     GGYTAFRVDITNLVDFGKSNRIDVMVDNSYSTEIAPISGDFSMMGGIYRDVYLEVSEK
                     IHFDKMNYAGQGVFVKSFLDGANAGFQIDANIVNSSGETKKLMLRTRITDQKGKIISA
                     QISKSINLKNAMQQNVKQLFAIPDYQLWSPDNPYLYTVECEILDAKTKTVLDTYCVRS
                     GIRTIAKDEKGNILLNGKVIKLIGVNRHQDFDGLSNALPDAIHRRDVEIIKEIGANFL
                     RTSHYPHDPAIYDACDELGILVWSEISVVNQIGINEHFEQNTLNNMKEMILQHFNHPS
                     VVFWGFMNEVLLAANSFPENERGNLFVKTRELAQKLNDVAKSLDNTRLTTIAHHGDFE
                     KYEKAGLNNITDVCGYNLYFGWYVNGFGGFDDFVDNFKQKYPTKPLIISEYGAGSNKG
                     IYARLPRRFDNSMQWAEKLHEIYLDKIIRNPKIAGSSLWNFADFSSEGRKDTDPHMNN
                     KGLLYYDRTPKDVFYLYKAKLQNEPALKIAAGKWLKRPVEVPVEARTFTDTIKIYSNC
                     QQVTLQLNGKTVGNFQPDSICRIFAPLNFVNGKNQLVAIGRSTNGKTITDVVNFDYHF
                     IPFPLNNNSFRELCVNVGGHFDYVDTKSNEVWMPDRPFRPGGWGYVGGEEFKIWKGQR
                     TGGERVIYNTINDPLYQTQRVGLTEYRFDVAAGWYEVELLFAELQSAKELKDAFLNNL
                     GNETKTELLKDGRIFNVSINNQLVLSELNLLESVGENTAANFTFRVKAADNGVVVKFD
                     KIRSETVLNGIKIRKF"
     gene            complement(75514..75663)
                     /locus_tag="PLM04_00660"
                     /pseudo
     CDS             complement(75514..75663)
                     /locus_tag="PLM04_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT        23527 a        14472 c        14263 g        24306 t
ORIGIN      
        1 gaaaactaac attcttattt gcctgcctat ttatgatagg agttggtttg gtcaacgccc
       61 aatctctgac tgtccaagga aaggtgattt cggatgaaga tgggcaacct gttattggcg
      121 cctctgtact ggtaaaaggt accactactg gcacaattac cgggactgat ggaagttttt
      181 ccattactgt gccggctggc aaccgaacac tggttgtttc ctacattgga atgaaatcag
      241 tggaagttcc agctgaaaac gggatggtag taaggctgca acctcaaacg ttggaaattg
      301 acgaagttgt ggttacggca atcggcatca aacgctcaga aaaagctttg ggatattcgg
      361 cttcggcagt gagctctcaa gacattacca tgaccggaaa cagaagtgca ttaaatgcat
      421 tgcaaggtaa ggtagcgggc gtcgaaatta cttccgcttc tggagctccc ggatcttcta
      481 ctcgcgttat catgcgtggt tactcttcat tgggaggaag caatcaacct ttgtatgttg
      541 ttgatggagt tcccatcagc aatggacaaa ttggatcgac aagcataaat ggaggattgg
      601 attttggtaa ccgtgccaat gatattaatc cggaagatat tgaatcgatg actatactta
      661 aaggcggttc ggctactgca ttgtatggat ctcgtgcagc ttcgggtgtt attttgataa
      721 ctaccaaaaa aggtgaagga ttaaaaaata aacctaaagt tgaagtttct tcaacttcta
      781 ctttcgactc tccacttcgt ttgccctatt ttcagaacga atttggtcag ggttggtacg
      841 atagaggtgg cggagaacaa gatttgatgg aaaatggaag ctggggaccc cgctttgatg
      901 gcaagattcg cccatggggc tttgtagttg acaaccaaca acaaataaaa ccttatgtgg
      961 ctttaccaac caatataaaa gatttcttcg acatcggtac caattataat aatacggttt
     1021 ctgtttcaaa tggcgatgct gataaaagct attatatttc ttacggaaat gtaacagccg
     1081 atggaattat gccaaccgat gctgattatt ataatcgtaa tacagtttcc atgcgcggtt
     1141 cggcaaagtt cctgaaaatt tttaatattt ccggttcgtt gaattatgtt cgaaaggata
     1201 tgagattcac agaaaccgga caggatcagg ctgcattgga tggactttgg caatcgccac
     1261 gcgatatcag tattgttgat ttaaaagatt acaacaacaa attcaacaat gtagataatt
     1321 attacacaat ctatgcccaa aatccttact atatattgaa tgaacatggc aataaatttc
     1381 aggaaaaccg catttttgga aatatcagcc tcgatgcgca gataacccca tcattgagtg
     1441 ccaccttcag attgggcgat gatgtggcca acagtacatt gaaaacatgg agagccataa
     1501 ccaacagtgc aagagctgat tacaacgatg aagtgggtag agtaggagaa aatgcttatt
     1561 actcatccga attgaatacc gatttctttt taaatttcaa tccaaaatta aataatgatt
     1621 ttgcgattag tgtcattgtg ggccataatt ttaatcagcg cgatgctcaa tctcaaggtt
     1681 ctgaagtaat tggcctcgat attcccaaat tctataattt gtcaaacagt tctgctactc
     1741 cttctattta tggaactttg tctcaacgta gattaattgg cgtttatggc aatttggatg
     1801 tgagctttaa ggatatgttg tttttgaaca taaccggtag aaacgactgg tcatctactt
     1861 taccagcaaa taatcgttca ttcttttatc caagcataag cggaagcttt ttgttttctg
     1921 aattgattca aaacaaagat gtactttctt atggaaaact tagagccagc tttgctcgta
     1981 ctggtaaaga tgctgaccca tatttaattt atcctgtact gcttcaaacc agtattacgg
     2041 atggctatcg ttctttggat tttcctttgg ctggtcctgt gaatggtttt accatttcca
     2101 acgttattgg taacgaccaa ttacaacccg aaatatctca agactttgaa ataggtacca
     2161 atttgaaact tttcaaaaat ttattggata tagatttcac atggtacaac aaaaaaatca
     2221 ctgctttaat ttggactgca acccttccat cttctaccgg ttatacttca caaactcaaa
     2281 acctgggaga aattaccaat aagggagttg aactgagttt aagtgtgaca cctatacgaa
     2341 gaaaaaatat agaatgggag gtttttgcca attattccaa aaacaacaat ttacttaaat
     2401 ctttaatttc tggtttggat gaaattgtgt tgggaggaac aagaggcttc ccatttgtgg
     2461 ctcgtccggg ctatgaattg ggacttttta aaggcaatgt tcctgagacc gatccacaag
     2521 gacgtattgt tgtaaatgca caaggattac ctgttttcaa agccgaacag gaaattattg
     2581 ggtcttctca aaataaatac cgactgggtg gcggaacttc tttgaaaatt aaaaatttca
     2641 gggtacatgc tgcatttgat tatagaaatg gaggaaaaat gtattcccgc acagcagaaa
     2701 ttgcttattt tactggtaat gcacaaccta ctacttacaa cgacagacaa ccatttatta
     2761 ttccgaattc tgtacagtta gttggaggac aatatattga aaacaccact cctatcgcag
     2821 gttttactaa caacttgaat ttgtattaca atcaaactta caaggcagga attggggatg
     2881 cttatgcgtt aattgacaaa acattcttta aactgagaga actcagcatt tcctattctt
     2941 tcccgaaatc ggttttgaag aatgctttta tcaattcggt tgatttgtct ttggtgggaa
     3001 ataacctgtt cttgtggact ccagactcga atttgtacac ggatcctgaa ttaactactt
     3061 ttgggaacga tttggctgcc gattatggtg aattcggggc aactccatca acaagaagtt
     3121 tgggatttaa tattaaattg agtttctaaa aaacataaaa acgaattata tatgaaacga
     3181 ttaaaatttt ataatatagc tgcttttatt ggcttgctat tcttaagcag ttgcagcgaa
     3241 agcttttttg acataaatca tgacccaaac aatccatccg aagcaactcc gggattagta
     3301 cttccaagtg ctttggctaa ttcggcctat gtaatgggtg gttattatca tgctttggga
     3361 agtttctgga cacaacaata tgctcaggct cctgcagcgt ctcaatgggc tgattgggaa
     3421 tcctacaatt tgacaaatgt ggactttgat cgtcaattta cacttttata tgcaggcgga
     3481 ttaaatgatt acgaatatgt tcgtaaaaat actgccgaga ctgggaactg gaaattctat
     3541 gccatcgcta ccttaatgca ggcatatact tttcaggtat tggctgattt gtatgatcaa
     3601 attccattta gagaggcttt aaaggggaat gttatacctc attatgataa tggccagctg
     3661 gtttatgata gcctacttgc acggattgat tatgcaatgt ctaaagattt taccggaagc
     3721 acagttgaaa atcccggctc ttctgatctc atttttggcg gaaatataga tgattggaaa
     3781 gcatttgcca atacattgaa attaaaaatg tatttacgat atgtgaatgt taatccaaac
     3841 acatatagaa cagagattga agctttgttg gctgaaaata agtttttgac aaaagatgcc
     3901 aagtttactg cctttaaaga ggaacaaacc ggatataatc ctttctacaa tacttttgtt
     3961 gatcgattgt caggtaatgt tgtagccaat aaaactttta ttgacttgct aaaagataag
     4021 aacgatccac gaatggcaaa aatttttaat ccttcggtta ccggtagctt gtataatggt
     4081 attgctgcag gtgataataa aaatcacccc actgaaacca gtaaaaatta tgcgactcca
     4141 gctattaagg gtacaagtcc cgtttatttt ttctcaaagg aagaagtact ctttttgatt
     4201 gctgaagctc aagctcgtta tgggacggct tctgatgctg agagcaccta taaaactgcc
     4261 atacgggctt ccttgacaag tctcggttta gcagcagacg ccatcgacat agatgcaacc
     4321 tatccctata atggaattcg gtcgataatt gagcaaaaat ggatcgctca agccaataaa
     4381 aacggaatag aagcattttt cgattacaat cgtacgggat atcccaattt cttcactcgt
     4441 tcacttactt ctgttttgaa tggagatgaa agacctaaac gcttgtactt ccccagcaga
     4501 gaagtcaatt caaaccccta tactcctgca agagtggatc ttacagaacc tgtatggtgg
     4561 gctaagtaaa acaattaaca attaaaatta attcgttatg aaaaaaataa tattaatgac
     4621 tttggcattt gtggcttttg tttttacttc ctgtgaaaaa gagtcgcttg gtgtttcaag
     4681 agttacaact tatgctacta tggaattgaa tgggcctgaa gaattgtttt ggcctttaaa
     4741 ttcccccttt gtagatccgg gatgtaaagc ttttgaagga acaactgaca tttcaggaaa
     4801 tatagaggtt atttcaaatg taaatgcaac aaaaggaggt aaatattcca ttacctataa
     4861 agttaaaaat tctgatggct tttacgctca aacatcgcga acagtgtatg tatatgaaca
     4921 aaatgcccct ttaaatgggt attatcagtc tcgtataagg cgaaataata atggaactat
     4981 agctaatcgt ggtccattta ctatccttgt ttttggagta ggtaatgatc aatattggat
     5041 agaagatcta ttaggcgggt ggtataatta tggttcagga tatggtccgg cttatgcagg
     5101 cccaggcatc attaagttga atagtgataa tacttttgaa attgtatccg ctgaacctct
     5161 cgcttgggga tatccatgta ttttaactga aaatacagta tccacttatg atccttctac
     5221 aaaaaccata gtgcttaata caagaatgaa agatacccca ctaatgttgt ttacggtaac
     5281 attaactaat ccacaaccat taaactaaag gagatataac tatgcgtata aataaatttt
     5341 ttatcctatc tattttatca ttggctcttt tttcctgcga gccaaagtat gaaaaggaat
     5401 ataattgggc ttatcctgtt tgcggcgatt ggatggtaaa agcctatttg gatggaacag
     5461 aaatatatgg gccatttgaa attatgactt ataatacttc gtttggtcaa gattccatct
     5521 ggattcacga ttatgctacc actgctacta atggacattt ttggtcgttc aaagtgaagg
     5581 cggccgtaga tatgaagaca cttacgtttc aaacagaagg ctcaattaat gctattgcag
     5641 gttatcccat tgtaatcaaa gtttctaatg gtcgggtaat caacaaggac agcataacta
     5701 tggaactaga gtttgaagat gatccgggaa caatttatca attagccggt catcgtgaaa
     5761 cttcctacga agaatacatg gagcaataaa catgctgtcg attaattatt gaaaaggctg
     5821 cggaaaaccg cagccttttt cattcctgta cttaacaatg tgtttatttc cgcactatcg
     5881 cttccaaatt cttgtttcgg atttgaatat aaattttcgt accgggttgc gcatattccg
     5941 ttgccacata tcccatcccg attccctttt tgctatcggg cagcatggtg cccgaagtaa
     6001 cctgtccaac gggtttgttt tcattgttta caattgggta gccatgacgc ggaatacctc
     6061 gttcggtaag ttcaaaacgt accagttttc gcgttacgcc ttctcttttc tgcttttcga
     6121 gcaacggaca gtcgatgaaa ttctttcctt ctacaaattt ggtaatccag cccaaaccgg
     6181 cttctatggg agaagtggta tcgtcaatat cgttcccgta aagacaataa cccatttcga
     6241 gccgcaaagt atctcgggct cccagtccaa tggggcgaat gccttcctct gctccggctt
     6301 caaaaatggc attccatagc gtatcggcat actcgggacg gaaataaagt tcaaatccgc
     6361 cggctcccgt ataaccggta ttggacagca acaccggctc cacaccggca aacgttccta
     6421 cagcaaatga gtaataagga atttcggaaa gccgaacagg cgtgagtttc tgaagaattt
     6481 gcgtagcttt gggaccttgc acggccaatt gagccacctg ttccgaaata tttaccagct
     6541 ctgcattttc cgtattgttc tgcatgcacc aattccaatc tttttcgata ttggctgcat
     6601 ttaccaccag caaataattt tccttatcga ggcgatacac cagcaaatcg tccacaattc
     6661 cacctctccc gttcgggaaa caggaatatt gcgcttttcc caccgggagg gcagccacat
     6721 cgttggtagt cattttttga agaaaagcca atgcatgagg ccctttcacc agaaattcgc
     6781 ccatgtggga cacgtcaaaa acgccggcag cccgtctgac agtcaaatgt tcttcgttga
     6841 tgcctgtata ttcaatcggc atgtaaaaac ctgcaaactc ggtcattttt gctccaagag
     6901 cgatgtgaaa atgcgtaaat ggtgtaatct tcataagctt ggttgaattt gtatgaaaaa
     6961 aatcgatact tgtttttatt tgaaaattaa atgataatcg attggatgta cttgcgtttg
     7021 gagtattatg atttttcgta gttagtcatg aaaaaaatat tcaaaaacac tttgcccgac
     7081 aagaccaaac ttttccactt tattggaatg tatcagtttt gggtttatga tttgttagta
     7141 taaggcattt aatgttaatt tcaatgcaaa ttttaataaa tacaaaagta tgaaaaaaat
     7201 taatttgttg gaactaattt agtaccgtaa gttcaaaagg atttttttat ctaagaaacc
     7261 cacatgtatt gcatacacaa acacgaatga atataatatt cagacatgtg ggttccatca
     7321 gacctttaaa aaataaatta tattctgatg aaaacggaca atcacagtct gatggatcga
     7381 tgagaggaat aacgggaaaa gggatgagtc gtgtttgaca caggtatcaa aaaaaaccaa
     7441 cctgaaagca acccatattt tgtgtttttt aacccaaata attggagttg accatatgta
     7501 gcaaacaatt ttttccatta aaatgtattt tttacaaacc gaaaaataca tgcatttaac
     7561 tgtaaaataa attcttatgt atgtttattt tagcggattt ctgcatgttg atggtttgac
     7621 ttgttagctc ttttccatct gaattaacta atatttgtct gtgcgtcgat ttgaaatttt
     7681 catgtatctt tgcagaagca ttggtttcaa tcaaccatat ccggtcaatt gatgaaaagg
     7741 gaaagccgtg tcaatcggct gcagtcccgc tgctgtaagc tcttgtagtt tttttgaaca
     7801 atactgttgc cactgttcag gggttgtcga aaaaaataat atatcttttt ttcaatttcc
     7861 cattacggga aggcgttcaa aaaaaagagc aagccagaag acctgccatg cttttattca
     7921 gttaaaaagc tttcgaggaa agagctttaa ccatctacac atttttattt atgttgattc
     7981 tcttttttta atgaggctgt ttttgtaaat gattacaaac cggttatgca caaaaatttt
     8041 ttcttttatc tgatagaaat tttttttctg ttttttttta ttcctgtaag agcagagata
     8101 ttatttcagg atatgggtat ggatacggca aaaacatatt ccattcccga aatagtagta
     8161 gttgaaaaat ttcaaaacag cgaaattcgt tcaactatgc cattgcagat tctttcggca
     8221 aaaaaattag aaaatctgaa tgtattgcaa ctttctgatg ccataaaata ttttgcgggt
     8281 gtcaacgtaa aagattatgg aggtattggc ggtttgaaaa ctgtctcagt tcgaagtctg
     8341 ggtgctgaac atacagcagt gagttatgat gggataatgc taaccgatta tcagacgggg
     8401 caaattgatc ttgggcgttt ttcgcttgac aatgtggata tggtctcatt aaataatgga
     8461 caaagcgaca acatttttct tcccgctcgt tcttttgctt cgtcttccgt gttaaatatt
     8521 cgcaccttac caccacgatt tgaagaggaa aaatcgatac acggaaaaat tacctgcaaa
     8581 gtaggttctt ttggtcttat taatccttct ctttttatac gacagcaaat gagcagaaaa
     8641 atttctgccg tttttagtgg tgaatggctt actgctgagg gtgagtatcc ttatctgctg
     8701 atttatggta cgcagacagg ggacagttct tcaatagaaa caagaaaaaa taccgatgtt
     8761 gaaaccttgc gattggaagg aacaattttt gccaattttt ttcatcgtgg ttcaggctat
     8821 ctgaaaacct atttttatga atcagaaaga ggcttgccgg gggcagttat ttattacatg
     8881 aacgaaaata ccaaccatca gcgaagcaaa gatcaaactt tttttactca ggcacattat
     8941 gaaagtagct tttcttcctt gttatctttt caagccaaca ccaaatatca aaatggttat
     9001 ttgcattatg tcgatccaaa ttataacaat tcgggaggaa aaatagacaa tacatattca
     9061 caacaggaat tttacttttc tgcttcgttc ctttacaagg ctttcgaaaa tttatctttt
     9121 tcagcatcaa cagattattc gttgaatacg ctgaatgcca acttgccgga atttgccttt
     9181 ccgaaaagaa acaccgtatt gatggtttta aacgccaaat atgtatccaa tcaatttttg
     9241 gctactgctg gtggtttaca aacatttgtg aatgaaaaag ttaagtttgg atcatctccc
     9301 aacgacaaaa agcatttttc accttttttc agtttgtctg ttaaaccttt tgaacagtat
     9361 gacttcagga tacgcagttt ttataaaaat gttttcagaa tgccaacttt caacgatctt
     9421 tattattcat acgtgggcaa cagaaatttg gagcccgaaa atgcgcaaca attcaatgcc
     9481 ggcattactt ggtctttttc atcggaaagt atttcattgc tttcagtaac attagatgcc
     9541 tttcacaatg ccattagaga taaaattgtt gcatttccta acaaggatat ttttaactgg
     9601 acgatgaaaa attgtggtaa agtctctatc aatgggttgg atgtggcaat ggaaagcgga
     9661 atttcgataa caaggaatat tggactggta tttggaggat cgtatactta ccagcaagca
     9721 ctgaatgtta ctgatcctaa cgccgaagat tacctgcatc aaattccgta tacgccaaga
     9781 atttccggtt ccggaaaagc tgctttggaa atgccttggg ttaatctttc ttattcattt
     9841 atctggtcag ggcatcggta taccttgttt caaaattatg cttccaaccg tttgccgggt
     9901 tatgccgatc ataatttgtc gctttcacgg agttttaaaa aatcatctta ttcctgcttc
     9961 ttcaagctgg aaataaacaa ttttctcgac aaaaattatg aaattgttcg aggttttccc
    10021 atgccgggac gttctttcag atctattgtt gcgatagaat tttagttaac ctgcaaagaa
    10081 gattttttta aagcagtaaa acaggtaaaa aaacgcataa aaaacccaaa gcaaaaccgg
    10141 ctatggtttg cattggagtg tgggccttga gggcaacacg tgaaattgca gaaagcgctg
    10201 ccaaaagcag tataagaata aaaagccaaa gaggatttat ggaatatcgg taacaaattc
    10261 cgaatattcc tccggccaaa cctcccaaac ttgccagatg agcactgatt ttccacttga
    10321 cattgataaa agtaataaga agaatggata tggaagaccc aatgcccaat ccgccaataa
    10381 acattggcaa gtgcatggta cgtaccagga aaagcgtcca gaacacataa gccaaaaagg
    10441 aaaacagata aggtagggtt ctttcttcgc gattggaaat aaacaaatcg ctcacttcct
    10501 tcctaaagta cattataaca acaggtatag acggcaacaa agcggtgaac aaaaatgttc
    10561 ctccaacagc aatggctttc cactgcatcg gaaggtttgc aaaaacggga atttgtatca
    10621 gcagcaatat gccataagtt ggcaacaata taggctgtaa aataatcgaa ataattttat
    10681 agaggatttt cataaatgtt atattttttt aaaaacgaca aatctttaaa ttcatatttc
    10741 ttttcttaat cgagccaccg gaatatttag ttgctctcga tatttagcaa ccgtacgccg
    10801 ggctattaag tagccctttt gttttaatac ttcagccagc atttcatcat ttaatggatg
    10861 tcgtttatct tcgttatcaa cgcattcctg aagaattttt tttatttctc gagtcgaaat
    10921 ttcttctccg gcatcgttgg tcatggcttc agaaaagaaa tatttcagag gaaaaactcc
    10981 aaattctgtt tgcacatatt tattgttgct tactctggaa atagtagaaa tgtcatagcc
    11041 ggttctgtcg gctatatttt taagtatcat tggttttaga taaatttcat ctccttcgat
    11101 gaaaaaatct ttttgaaatt cgacaatggc agtcatggta gtcaataatg tctgctgcct
    11161 ctgttttatg gcatcaataa accaacgtgc agaatcgatt ttttgtttga caaacagaat
    11221 agcttctttc atctgaggtg tctgattttc cttgttggaa gtataatcct gaaacatttc
    11281 ggaataagca gcattgactc gcaaataggg aatattgctg ttgtttaaat gtacggttag
    11341 ttcaccatcg ttattttcca caataaagtc aggtacaata atcgacatgt ttttttccaa
    11401 gatgtttccg ctccatgcat tgccgggttt cggattcaga cgaataattt cgtttatggc
    11461 attctttagt gtattaccgt caatttcgag gccgtttata attttatcgt aatgcttttt
    11521 tgaaaattct tcaaaatagt cttgaattat gcgttttggt atttcaattt ccttctcgtt
    11581 tcttttcttt tccaactgta gtaacagaca ttcctgtaaa gaggaagctc ctacgcccgg
    11641 aggatcaaac tgttgaataa ttttaacgat tttttctatt tcctttacat tagtgtccat
    11701 attggcctga aaggctacat catctgccaa tgcttcaggt gttctacgca aataaccttc
    11761 ttcatcaata ttgcctatga caaactctgc caaagccctg tccctttccg aaagcggaac
    11821 aagattcaac tgttccatta agaaatcatg aaaagtggac ccggcggaga aaggaatttc
    11881 ttcgtatttg tcgtcttttg aagtgttgtt tgcctttaac ttgtaatcgg gtatgtcatc
    11941 atccgacata tattcttcaa aatcaatttc atcgttgctt tctccctcgt catttcctgt
    12001 ttcttcgtaa tcttcaccaa agtcatccaa ttcctttttt tcttcttcgg gttctaagcc
    12061 tatttccaaa actggatttt cttccaattc ctgacgaata cgttcttcaa gttccaaagt
    12121 aggaatctca agcattttta ttacttgaat ttgtgccggt gaaagttttt gttgaagttt
    12181 ttgttgtaat tgttgtttaa gcattgaaat aatgattgag tttatgcaaa gataaatgtt
    12241 ttttgtaaat ttagttttta tggagtgttt tttgtctttg taatttttgc taaatatttc
    12301 gaaaagtttt tctgatacga tatataaaaa atacgaattt ttttaccaat tcgatgtttt
    12361 tttgttgttt tgtataaaac aattgttaat cttttactgt ttcctattca aaaagcaaac
    12421 taagatataa ttatttgtgt tatgttgcaa atttttcaac catttcgcat aaaggatata
    12481 actttaaaaa atcgtctggg catgtcaccc atgtgccaat tttctgcgca agatggattg
    12541 gcaaacgatt ggcatctggt tcattatgga acgagaggtg taggaggaat agctttaata
    12601 atttttgaag caacgggagt tagtcctgtt ggccgtattt cgcccgaaga tttgggattg
    12661 tggaaagatg aacaaatcga accattgaag aaaatagttg attttattca tcaacaggat
    12721 tcaaaagcag ggattcaaat tgcccatgcc ggaagaaaag gctctcgttc ttctcctttc
    12781 aaaggtgaaa aacagctcac caatgaagag ggcggctggc aaacgattgc tccttctgct
    12841 attccttttc gtcctgaaga aattcctcca catgaattga ccaagtctga aataaaaaag
    12901 gtagttaatc agttcaaaga tgcagcactg agggcgtttc aagctggatt cgatgttctt
    12961 gaaattcatg ctgcacatgg gtaccttatt catgaatttc tttcgccaat cagtaatttt
    13021 aggaaagatg aatacggcgg ttcttttaaa ggacgcatac gtttgttaca ggaaattgtt
    13081 tccgccgttc aaaccgtttg gcctgaaaga ttgccccttt ttgtgaggct ttcagctacc
    13141 gattggactg atcaaggatg gcgtttggaa gatacggttg agttggctaa aattctttat
    13201 ctggaaggag tagacttaat tgattgttcg tcgggcggaa ttttaccaaa tgtgcatatt
    13261 cccttagctc cttgttatca ggtcccattt tcggaacaaa tccgtaaaac aggcattctt
    13321 acggcagcag ttggattaat taccaagcct gaagaaatac aagatattct acgaaccgaa
    13381 aaagccgata tagtgctttt gggaagagaa ttactgcgaa atccctattt tgctcttcat
    13441 tgtgctcggc atgtaaaaga aaactcaaaa tggccacaac aatatttaag agcaaaattg
    13501 gaataaaatt tataatttgc atgcaaaaac acgagagaaa agagattttg aaatcaatct
    13561 attattatga aacccccatg tattgcgtac acaactacga atgaatataa tattcataca
    13621 ggtgggttcc atcagacctt taaaaaataa attatattct gatgaaagtt aatttttgta
    13681 ctttatttgt gctgtttaat atcttgtttt actcttgcag ccccaaaacg tggcaggtag
    13741 tggaagcaac atcagtaaaa atacctatcg acagtactac tgaaaagttt gccgacaaag
    13801 aatatgaggc tgctcttcaa ccggtgaaag agagaattga tgctgaaatg gctgttgtaa
    13861 ttggctattc agcagaaaat atgagaagtt ttgcaccaga aagtccactt tcaaattttt
    13921 gtgccgatgt gtttcgtcgt gcagcttcgg attttttaca tcaagaggta gatgtgtctg
    13981 tggtaaatct tggcggatta cgaactgaaa ttcctgccgg taatattacc attcgcaata
    14041 tctacgaatt aatgcctttt gaaaatgagt tggtaattct ttgggtcaga ggagataagt
    14101 taattcaact tttccaattt tttgcttctg tcggaggaga gggagtttcc ggtatctcga
    14161 tgaaaataaa aaacggcaaa gccacccaaa ttctggtcgg tacacatccg attgacccgg
    14221 aaaaaattta taccgtagct accagtgatt atctcgccga agggaatgac caaatgacgg
    14281 ctttatcaga atatgaaaaa atttcaggta ccggattaaa acttcgaaat gtccttatcg
    14341 atttcattcg gaatgaaact ttgaagggac atgttataca atccaaaatt gatggcagaa
    14401 taagtatgga aaactaaaaa ctatttttta tgaaaactat ccaaaatact gcgattcttg
    14461 tttttttgtt ggcgttcaac agtattcttt atggaggaga aagaataaag ttgaccattc
    14521 tccacacaaa cgacacccac agtcagatag agccgacgga aaaatcaaat cttcctgttt
    14581 ccgataaagg cggttatgct cgtcgaatgg gaataattga acaaatccgg caacaaaata
    14641 aaaatgtgtt gttgttcgat gccggcgatt tttggcaggg aacgccttat tttaattttt
    14701 acaatggcag aatagaggtg gatgggatga acaaaatgaa atacgatgct gtaactttgg
    14761 ggaatcacga attcgacaat gggttggaca ctatggcaac ggtgttgaaa aacgctcaat
    14821 ttcccatatt aacttccaat tatcagatgg acagcactgc aatggcaact ttggtaaaac
    14881 cttacctcat tattcgcaaa gctggtttga aaatcggagt aatggctttg aatgtcaatc
    14941 caaaaggtct gattttcacg aataactaca agaaaatgaa ttacatcgat cctgtggaaa
    15001 cggctatcaa tatctccaac tttttaaaaa gaagcgaaaa gtgcgatttg attatttgcc
    15061 tgtcgcattt gggtgccgac acgctgaagc aaaccctgac cgactacgat ataatacgta
    15121 aaaccaggta tattgatgtg atgattggag gtcattctca tcaaattatt actcaaacca
    15181 acttaaaaaa tgcagccggt aaacctgttg tattggcaca gatggggaaa agcggacttt
    15241 atctgggaag aatagatttg gaactcgaga aaaaatgacg taaaaattta tttgttcgat
    15301 ctcagcggat gcgtttgcat cattacgaaa gaccctacaa acatgggaat tacaaaatta
    15361 atgacccata taaaagtact tgccagaata atttgaaccg tttgacctga gaaggcgccg
    15421 ataaccaaaa ccgaatagga gcttcggatg gcagcttctg aaaaagccat agaaggagta
    15481 aaagttacaa acaagtaagt agtgggaata gctattgccg cagccaaacc gttgatttct
    15541 acgccaaaaa atcgcaacat cagataaaac tgaaaagaga aaaccaaata tcttaaaaaa
    15601 gaaagtagca gaatttttaa taaatcggca agagaataat cggccagaca agcaataaaa
    15661 tttatccatt ttgctgacaa ctttttttgt tttattcgcc caaccaatgg gggcagagaa
    15721 aaataaattc cactcaataa caacaaaatt ccgcaaacta tcagaaaata tccgaaaaaa
    15781 ttattctctc ctgcaaattc agagttttta ttactcaaaa aaaataaaat ggccgccgga
    15841 ataccgcaaa tagccattat gatgttttgc gtaatactgt taacaaaact taatgttatt
    15901 cctgctttcc tgttttcggg ctgcaggtag gcaattctgc cggccagttc tcccacacga
    15961 ttcggggtga agaaggcagt aagaaatccc gccataacac tctgaaaagc caaagccata
    16021 tttatttttt ccgaatttct cactaaccgt tgccatttca tcgattccaa tagccaattg
    16081 gcaggcaaca acaaaattac accccacaaa taccaagttt tccaaaatga tatttctttg
    16141 aattgagaaa gcaatcgccc gtaatgttga aagcaaatca gccggtaacc caaaaaaagg
    16201 aatgctgccg tcaggcaact ccacttgaaa atgttccaca acttcgattt gtcggttgtc
    16261 attttagtgg ttacagccat gtttttcata ggttgaaaca agaaaatgtt gttttaatga
    16321 ttcgactctt tgttcaaaga cagagaagcg gttttgaaag tgaatttctt ctgcgcaaaa
    16381 tagctgaaaa gtgttgttat aattgtgata agcattttag aaggagtagg ataaaaatgg
    16441 gcaatttcga caaaaaactt caaaccggca taattcaagc ccagatttat aatgacgacc
    16501 aacaaatacc ggcgaagttg aacatgacct cgcagatagg aagcagaaaa agttacgtat
    16561 ttttgcaaaa ggaaacccgt catgaacgaa acaggaaatg tcagagcaag tgccgcaata
    16621 tgcgaactca tggttataaa acccaagtga agcatttgat ggcgaagtac gaaattgtaa
    16681 actacaaaat acaataccca gtcgaaaacc atgtttgcag ctccggatac tccatagcga
    16741 aaaaactgta aaggcatgaa tttatggaaa ggcgggtaaa aaaaatcgat taattttgta
    16801 atcgagcttc ctgtgtttcg aaaaaatttt ctcattaaca acaattaaat gttcaattcg
    16861 ggcaattttt ttttataaat aaaattatca aacaattttt gattaatttt gtggcgaaaa
    16921 aatttctaca aagatacgaa ttaatttaaa ttccattttt tagtttttct catttttatt
    16981 tattcagtta aatactcata tgtctgacga ttatcacagt aatgacttta gtaagaccaa
    17041 aaaaatactt gggtcaacat ttcctgaaaa gcatggatgt tgcccgtcgt atagccggca
    17101 gtttacccgt cggcagtcct gcttcagtat tggaaatagg gccgggcatg ggtatgctta
    17161 ctcagttttt gcttcagcag ccacaaattg atcttaaagt tgtagaaatt gaccacgaat
    17221 cggttgttta cctgcagcaa cacttccccc agcttcaaat tttagaaaaa gattttcttc
    17281 aacttgactt aaatcagatt ttttctcatc ctttcagcat aataggaaat tttccctaca
    17341 atatttccag tcagattttc tttaaagttc tcgataacaa agatttaatt ccttctgttg
    17401 tgggaatgct tcagaaagaa gtggccgaac ggctggcttc tccacccggc aataaggctt
    17461 acggaatact aagtgttttg ctgcaggcaa attacgatat caactatctt ttcactgtag
    17521 acgaaactgt tttttaccct ccacctaaag taaaatcggc tgtcatcagc ctcaagcgca
    17581 aagaaaatcc cgaagtggac tatgacgaac aactattcag gcgagttgtg aaaacggctt
    17641 tcaatcaaag aagaaaaacg ttgcgtaatt cattgaaatc aatcattccc tttcatccca
    17701 tgaatgcaga tcctcttttc gatttgcggc ctgaacagtt ggatgttaaa acatttgttg
    17761 agttgaccaa taaaattggt tcggctttga aagaaaaaca ataattcgtt tatccggtga
    17821 caaaatagcc accactgctg cttcgaaaca gcagaaaaaa aagaaaggag caaaattatg
    17881 tcggaattaa gaatttttta ccacgaaaat gatgggaaac tgaaaatttc caaaagtatc
    17941 gatacgctca aaaagatcaa ccttaaagat attatttgga ttgacatgaa tgatgtttcc
    18001 gaaaatatag aatcagaact cgagcaattt cttaaaattt acattcagga agatgaagaa
    18061 attgaagaaa ttgagatgag ttcccgctac attcagaccg atgacagcat tgtggcgaat
    18121 tccaattttt tgcaggacaa ttatgttatg cagccggttt cgtttatcat aaaaaacgga
    18181 attttggttt cggttcgcaa taccgagctg aaatccttta acgaaaccgt aaaaaaaatc
    18241 tttgtcaata ccagtaattt tccttcaggc tatcatgtga tggtttctct ctttgaaacg
    18301 cgagtagaat acgatgccga catgatagaa gatgttaccg accaaatcac caatttaagt
    18361 aagaatttga atacggaatc cgatctcgat caagacattt tactgcaaat aaaggaatta
    18421 caggaaaagg taatgattat cagacaaaat attgtggata agcagcgtgt ggtttccaac
    18481 atgctgaaat gcaatttctt tcccgaagat ttgataccgc gtcttaacat cattctaaaa
    18541 gacattaatt ctttgtttga atatacccgt tttgatttcg atcgactgga ttatttgcag
    18601 gatacttttc tcgggttagt aaatttacag caaaacaaca ttattaaaat cttcacggtg
    18661 gtttccgtta tattccttcc acctacgctt attgcaagca tgtatggcat gaactttaaa
    18721 tacatgcccg agctcgattg gaaatttggg tatccttttt ccattttatt gatgatttcc
    18781 ttcacggtag gagtcttggt atttttcaaa aggaaaaaat ggctgtaaac ggcaagatga
    18841 taaaattttt tgtcgaaaat ataattattg agtctcctcc gaaaagtttc tcaggaaaag
    18901 agttttaaaa aatggataaa aagtgagaaa cgacaaaaaa tcgaacattt ctcttcaaaa
    18961 aatcttattt ctcccttctt aagaaaataa taaggtttta agtaggggtt cattatttat
    19021 ttgcgggtac aggtaaattg actgtagcca aaacaaaaaa cctatccgta accgacagaa
    19081 aattttaatg tgcctcgagc cagttgttgc caacgccaca atctacaatt aacggaacac
    19141 tgagaggaaa agcatgttcc atttcgtaaa caacaagttt gcgcaccgtt tccaattcgt
    19201 ttacaggaac ggtaaaattc aattcgtcgt gcacctgcaa aatcattttt gcctgcaaat
    19261 tttctttttc caattttcgc tgaatgttga ccatcgcaat ttttataata tcggctgccg
    19321 agccctgaat cggtgcatta accgcatttc gttcggcaaa acctctcacc acgctgtttt
    19381 gtgaattgat atcgggcaaa aagcgttttc ttccgagcag cgtctccaca taaccgtttt
    19441 ctttggcttt tttgatggca ctttcgatgt aagtttttat ctgcggatag gctagaaaat
    19501 actcatcaat cagagttttg gcttctgtgc gtgaaatgcc gagccgttcc gccagaccaa
    19561 aagccgaaat tccgtaaata attccgaaat tggctgtttt ggcatgacga cgcatttcgg
    19621 gagttacttt ttccagcgga actttgtaaa ttttggctgc tgtagcggca tgtatatcca
    19681 aaccgtttct gaaagcctct atcatatttt ggtcgttgct caaatgagcc atcacacgaa
    19741 gttcaatctg cgaatagtcg gcactgagaa acaagctgtt ttcctccggg ataaaggctt
    19801 ttctgatttc ttttcccaat tcgtcgcgaa tgggaatatt ttgcaaattg ggattgctcg
    19861 aactcaatcg accggtggcg gtgttggttt ggttgtaaga tgtatgtatt ttccccgttt
    19921 tcggatgaat cagttgagga agcgcatcaa tgtaggtgct caacaatttt ttcaatcctc
    19981 tgtattccaa tattttatcc acaatcggat gtttgtgccg aagtttttcc agcacatctt
    20041 ccgaagtaga atactgtccg gtctttgttt tcttggcttt gtcatccaaa tgtaaccgat
    20101 caaacaatac ttctcccacc tgtaccggcg aattgatatt gaactcaccg cccgccattt
    20161 cataaatttc cttttcgagt ttcaacatat tttccgttag aatttccgaa ctttgacgca
    20221 aagcttcact gtcgatgcgt acacctgttt tttccattat taccaacact ttcatgagcg
    20281 gcatttcgat ttcataaaaa agtttttcga gctcgttggc cgccaattcc ttttccaata
    20341 tctgtttcag gcgaaacgta acatcggcat cttctgcagc atattccgta agtttggaaa
    20401 tttccacgtt tctaatatcc aactgatttt tcccttttgc tcctaccaga tcttcgtaat
    20461 gaatggtatg gtagtggaga taaatttcgg ccagataatc catgccatgc ctcaattcgg
    20521 gttgcaatag ataatgggca atcatggtat cgaaaagttt tccctttaca tcaatgccat
    20581 agttggagag catcagaagg tcaaacttca tgttgtgacc aattttttcg atttgttcac
    20641 tcagaaaaaa cgctttgaat tcgttcagaa tttttgttgc ttcttccctg ttgtcgggta
    20701 acgttacaaa ataggcttct ccttctctga aacagaacga aaggccaacc aaatcggccg
    20761 aaaaaacgtc aagtccggta gtttcggtat cgaaacaaac cgatttttgt agaaaaagtt
    20821 gcgaaacaag gtaggaacgc ttcactttat cgtcgatcaa ctgatagcga tgcgggatat
    20881 ttttcaaggc cataaatccg ttggaagcaa ctggtgaaat ttcttcaata ttttcttcag
    20941 gtttcgacag tattttttcc ttttcgggtt gagttctttc aggtctattg gtttctccga
    21001 aaagattcat ttgctcggcc ttggaagatt ttttatttcc tgaaggggtt tgaagtagcg
    21061 gataatcggg cggaaaagaa gaaaacgtat tgccaaattt gttgttcaat aaggtacgaa
    21121 attccaattc ctgaaacaga gcacgcaatt tttcgctgtc ggcaggtcca actttgagtg
    21181 cctcctcatc caaagcgacc ggcacatcgg ttttaatggt agccagaaat cgggaaaaat
    21241 taatcaagtc tttgttttct tcaattttcg ttttaagcga gcccttcaat ttgtcggtgt
    21301 tttgtagcaa attgtcaatg ctgccaaatt cctgaagcaa tttcacggcc gttttctcgc
    21361 ctatgcccgg gcaaccggga atattgtccg aactgtcgcc catcaatccc aataaatcaa
    21421 tcacctgctg ttgattttcg agttggtatt tttccttaat ctgatccgga cccagtactt
    21481 caaagccacc gttgtgatgg ggacgaaaca taaaaatatg gtcggaaacc aattgtccca
    21541 aatccttgtc ggaagtcagc aaataaactt caaatccttt cttttcggct tgcttggcaa
    21601 gtgttccaat cacgtcatcc gcttcgtaac cggtaacttc tatgacagga atgttataag
    21661 cagcaaccag ctcttttatg atgggtaacg ctttccgtat atcttcgggc gttttttcac
    21721 gctgtgcctt gtactgttcg taagcttcgt gccgaaaagt ttttccttcg ggatcgaaaa
    21781 caacggccaa atgtgtaggc tgttcgtttt ttaaaacttc ttccagcgta tttacaaaac
    21841 caaaaatagc agaagtgttc agtcctttag aattgatgcg cgggctgcga ataaatgcgt
    21901 aataggaacg atagataagt ccgtgtgcat cgattaaaaa aagttttttc ataggttttt
    21961 gaattttaca cccaaaaaag tgagttgtag tcaattagaa aaaatgaaaa attctttatt
    22021 gcaatttctc taactgaaaa ttaaaaaatg caaagaagaa agattttcca aacaaatgga
    22081 gaaaaaaatc gtttttttat ttgagtgtaa aagtaattaa aaaaaaggca taattggata
    22141 gcttatgacc aacgaacgtt taaatttgat tacttttgca gattatttca tttacttctg
    22201 ttcttatgaa cctgatagaa aagattagaa agcccataga gaaagaaatt gaacagtttg
    22261 aaaaatcgat gacagaggaa ttacaaaccg aaaatccact gttaaagaat atcagcaatt
    22321 atcttcagtt gggaaaaggc aagcaacttc ggcctatctt ggtgatattg tcggcaaagt
    22381 tgtgcggcga agttaccgaa agtaccattt acggagcaat ttcattagaa ttgatacata
    22441 cagccagctt aattcacgac gatgtggtgg acaatacttt ccagcgaaga ggtcaaccat
    22501 ccatcaacgc gcagtggaac aacaaagtag ctatacttgt tggcgatcat atactttcaa
    22561 aatcattgag ttctggaata aaagccgata atataaaaat tctggaaaca attgccaacg
    22621 taggaatgaa tctcacagac ggagaactgc tacaattgga caaagttcaa catgcgaaaa
    22681 tctccgagat ggattatttt gaaatcatac aaaaaaaaac cgccctgctt ttttcttcgt
    22741 gtgctcttat cggaggcctt tcggtaaaag ccgacaaaaa tactttgaag catttatatc
    22801 actttggtga atatgcagga atatgtttcc agatgaaaga cgaccttctc gactattcgg
    22861 aaaacaatga gattggcaaa ccaacccgaa acgacataaa agacggtaaa attacactac
    22921 cactcattta cgcattaaaa aacgccacta accaccaaaa gcacgaaatt gagcaatgga
    22981 ttgaaaacag tgatttttcc gacttgcata tcgaaaaaat tgtacaattt gttcaggaaa
    23041 acggtggctt ggagtacacc ttgcaaaaga tggaagaata taaaaacaaa gccattgaag
    23101 aactcaacgg ctttgctgaa ggtgaactga aacagtccat gattctcttt gcggaattta
    23161 tgtcgaaccg cgattattaa agcattagaa atatttgatt tcttttcctt ctatcgaact
    23221 gagcagatga ttggccaatc ggctggcacc aaaacggaac aaccgattgt tgagccattc
    23281 ttcgcccaaa atgtgtttca caagcgtata atgtttcacg gcttcttctg tggtagaaat
    23341 tccacctgcc ggtttgtatc caattttttg gcctgttttt tcataccatt cctttatggc
    23401 atgggaaata atatacgttg cttcgagggt ggcggaaacc tgaatttttc cggtagaagt
    23461 tttaacgaaa tcggctcccg ccgacatcgc cagaatagca gctttcttga tattcacact
    23521 atttttcaaa gcgccggttt caagaataac tttcaaatgt gcattgcggc aggcagcctt
    23581 gatttccgac agttcttcgt aaacttcgcc gtaattgcct tccagaaatt tcccaacgga
    23641 aataaccaca tctacttccg atgctccttc attgaccacc atagaagttt cggcaatttt
    23701 aacttcaata aaagtttgag aagaaggaaa tccggctgct acggaagcaa tcccgacatt
    23761 ttcttcggtc agatgcgact ttacggccgg aatcagggcc ggataaacac aaatggcagc
    23821 cacattggac aaatgaggaa aagtccctct gaaatgattg actttttcaa ccattgaaac
    23881 gatttctcct tcggtatcgg tactattgag cgaagttaaa tcaatcaaat tcaaacattt
    23941 ggcatacaca tcctttgatg cattttcggc aaaatgttct gcaagaatat tgtctatatc
    24001 tatttttatg gcttcgtcgt caactgattc gcactggtaa agttctaaca actcttcata
    24061 tctgtctttc ataatgttgt gaatttaaaa aatgattaat acagcttttt attttgtatg
    24121 tggcgttgtg catcgcgaag ggttttcttt tcgatatttt tgctgaaagc atctgtcaaa
    24181 tcaacgcctg tttgattggc caagcaaagc aaaacccaga gaacatcagc catttcgtca
    24241 gctaaatcat acttttcatc ttctgccttg tacgactgtt cgccataagt tcgtgccatc
    24301 actcgcgcca attctcccac ttcctccgta agaatggcca tgttggtcaa ttcaccaaaa
    24361 taacgtaccc cgtaagtctt gatccaatta tcaactattt gctgagcttc ctgcagagtc
    24421 atgacaacag aaattattcg ttaagtcgtg caataatggt ttcgttgccg gtagtttttt
    24481 ctcccacctg aacaaaaatt tcactgtcca acggcagaaa caaatctacc cgagatccaa
    24541 acttgataaa tcccagatgt tcatttacat gacattcttt tcccggatga gcataggtta
    24601 caatgcgctg tgccaaagcg ccggcaattt gtctgaccag aatttgcgtt tttccgggag
    24661 tttctatcac aacagtagaa cgctcatttt ctctgctcga cttgggcaaa taagcggcca
    24721 aatgccgtcc gttatggtgt tcggttttta caaccgttcc cgaaacagga taccagttgg
    24781 catgaacatt aaatatcgac atgaaaatgg aaacctgaat acgttcgtcg ccaaaatatt
    24841 cgggttccaa agttttctca accactacaa ctgttccatc ggccggtgca atgacaaaac
    24901 tcggatcatc aatttccaca attcttttcg gattgcggaa gaaaaaaaca aaaaaacaga
    24961 gaataattgc agaaagaacc gtattgatgg ccattgccgg accaggaaat tccatgtaaa
    25021 taatcaaatt gacagcaaac agcaatagca acaatcctgt caaaataaac caaccttctt
    25081 tatgtattct gatacttttt ttcatctgtt taaaaatgat ttatgagatt tctctggtca
    25141 attgaaaata gtacaacaaa aaaaatttct ctttttgttt aaaaatacaa ttttagaaaa
    25201 ctgcctgaaa gtaaccctgt cgggatattt tgaacgaaga gaactcagtt tgcaaattta
    25261 cttaaaatac tttgttgcta caaggtacga tataaacttt caataatatg ccgaaaatga
    25321 ttaaatttgt actgattttt cacaaaaaac caattttatt taccgacaaa ccgctcattc
    25381 ttattttaaa atttttatga atctttcacc acttactgcc atatcgcctg ttgatgggcg
    25441 ttatcgttca aaaacagaaa aatatgccga atatttttct gaatttgctt taattcgttt
    25501 tcgtgtgatg gttgaagtag aatattttat ttcactttgc gaaattcctt taaaagaact
    25561 tcaatcggtc ccgaaagatt gtttcgataa gctgagggca gtttatattc attttcagga
    25621 atcggatgcc gaaaaaatca aggaaatcga acaaactacc aatcatgatg tcaaggctgt
    25681 ggaatatttt ttgaaacagc aatttgacct gcttggtttg agcgattaca aggaatttat
    25741 tcatttcgga ttgacctctc aggatataaa caacactgca gttcccgttt cgataaagaa
    25801 agcgctggaa gatgtttact accccgaaat ggaaaatcta ttgaaaaaaa tgcgcgagtt
    25861 ggccgacgaa tggaaagata ttcccatgct ggccagaact cacggtcagc cggcttctcc
    25921 cacgcgattg ggaaaagaaa taatggtttt tgtctatcgg ctcgacaaac agctttcact
    25981 gcttcgtgcg gttcctattt ccggaaaatt tggaggcgcc accggcaatt tcaatgcaca
    26041 ttcggtagcg tatccttcca ttgaatggaa aaattttgcc gataaatttc ttgccgaaaa
    26101 acttggcata caacgtgaag aatggactac ccaaatttcg aattacgatc atctggcagc
    26161 tttgttggat agcctgaaac gaatcgatac cattttaatc gacttttgtc gggatgtatg
    26221 gacttatgtt tcgatggaat atttcaagca gaaaataaaa ccaggagaag tgggttcgtc
    26281 tgccatgcca cacaaagtga atcctattga tttcgaaaat gcagaaggaa atttgggcat
    26341 ggccaatgcc gtactcgatt tcctatcggc caaacttcct atttcccgtt tgcagagaga
    26401 tttgaccgac agcactgtga ttagaaatat tggtttacct ttttcttata ttctgatagc
    26461 cacgcaaagt ttgatgaaag ggatgaataa actggttttg aacgaatcgg ccattcgacg
    26521 cgaccttgaa aacaactggg cagttgtagc cgaagcaatt caaactattc ttcgacgtga
    26581 aggttatccc gctccctacg aaaccttaaa agctttgacc cgcaccaacg aagtcattac
    26641 agagcaaact atccatgact ttatcgattc gttgcaagtc agtgaagaag tgaaaaacga
    26701 attaagaaaa atttcccctt tctcttatac gggaatctga ttattgtatt gttttacaaa
    26761 aagatatatt aaattcaatg tttaaatttt ttgaactgct caagcgtttc attcctcctt
    26821 acaaaaaata tgttttcctc aatctgttgt tcaatttatt gtcgacagtt ttcagtcttt
    26881 tctcttttgc tgccattatt cctgttttgc agattctctt tggtttgcag cagtcgaatc
    26941 tgagttatca ggcatggact tggggcgatg gatggaatca aattatttca gcgctgaaaa
    27001 acaatatttt ttactatctt gaacaaatga tgactactgc cggtccttcg atgactttgc
    27061 tttatctggg cctttttctg attgtcatga cagttttcaa aaccggcact tcctatctgt
    27121 cgtcgtatta cattattccc atacgtacag gtgtgttgcg cgatttgaga aatcaacttt
    27181 ataaaaaaat tctcagcctt cccatcggat tttttaccga agaacgcaag ggcgacataa
    27241 tgtcgcgcat gaccagcgat gttaccgaaa ttgaaacctc catcatcagt tctttggaaa
    27301 tgattttcaa aaatcccatc attattctga tatacttgac cgtaatgttt gccatgagct
    27361 ggaaactgac attttttgta ttgatacttt tgcctctcag cggttggttg atcgggatga
    27421 tcggcaaatc gttgaaacag cgctctacat tgggtcagca gcaaaccggc gaattgcttt
    27481 cgcaaataga agaaacttta agcggactga gagtgattaa agctttcaat gccgaaaaaa
    27541 aactggaaaa aagatttgca gctttaaacg aaaaacttcg ccgtaccctc aatcgaatca
    27601 accggcgcta cacgttggca catccactca gtgagctgct cggaaccgta gttattgcca
    27661 ttttactttg gtttggaggt gtactgattt tggaaaacca cagttccatc aatgctgccg
    27721 aatttattta ctatttggta attttttaca gtatcataaa cccggcaaag gatttgtcta
    27781 aagcaggtta cagcgttcaa aaaggtttgg cttcgcttga acgcgtcgat aaaattctga
    27841 aagccgaaaa tacacttccc gagcctaccg aatcgaaaaa aattaattct tttaacgatc
    27901 gcatcgaatt caaaaacgta aactttcgct atcaaagcga ttgggtgtta caagacatca
    27961 atctgaccat accgaaagga aaaaccgttg cattggttgg tcagtccggt gcaggaaaat
    28021 ctactttggt cgatctgttg cctcgttttt acgatgttac ggaaggagaa attctgatag
    28081 acggcatcaa tatcaaagat ctgaacacaa aagacatccg ctcattgatg ggaaatgtaa
    28141 atcaggaagc catcctgttt aacgatactt ttttcaacaa cattgctttt ggcgttgaaa
    28201 atgccacatt ggaacaagtg atggaagctg caaaaatagc caacgctcac gatttcatta
    28261 tggcttccga aaatggttac cagacaacta ttggcgaccg cggaagcaag ctttcggggg
    28321 gtcagcgcca acgcatcagc attgcacgcg ctattctaaa aaaccctcca attctgattt
    28381 tggacgaggc aacttctgct ctcgacaccg aatcggaaaa aatggtgcag gaagcgatag
    28441 aaaaactaat gatgaaccga actacactgg tcattgcaca tcgcttgtcc accattaaac
    28501 gggctgacga aatttgtgtg atgtccgaag gaaaaattgt ggaacgcgga aaacatgatg
    28561 agttgattgc tttaaacggc tattacaaaa ggttgtatga tatgcaatct ttctgaaaag
    28621 aattgagaaa ttaattcaaa aactgattgt tgaggtgagc aaaaaaactt tttgaagaaa
    28681 ggaacaattt ttgtaggaaa atttagattt tgttctctaa ttcatttttt ctgaatgagg
    28741 tttacagcga ataatttttt atatgaaaca tgggtgattt actttaaacc tctttcccgg
    28801 aattcgattg acacggcaat tttaaaaatt ttttgagcaa attttagctg caattaatta
    28861 acaaccaata taaaagatat acaatgtcga aaatttatga ttttacatta tcttatagct
    28921 tattcagaag ttatgtgatg tttgccttca aaaaattttt cagccgatac attgttatgg
    28981 gaaaagaaaa tattcctgaa accgggccgg tgatttttgc gcccaatcat ttaaacgcac
    29041 ttatggatgc gctggccgtt atttcggtag ttcctcataa aatgccggta gtctttttgg
    29101 cacgttccga ttatttcaat aataaactga ttgccagatt tctgcatttt tgtaaaattt
    29161 taccggcatt ccgcatgcgc gaaggtttag aaaacgtcga caaaaacaat gaaatttttg
    29221 aggcttgcgt tgatgttctg aatcataatc aggccattgg catcatgccg gagggaaatc
    29281 agggaagtaa acgaaaattg cgtccgctgg caaaaggaat tttccgaatt gccttcatgg
    29341 cacaacaaaa atatggcaat caacccggag ttaaaatcgt tcccgttggt ttggattggg
    29401 gaaatatcga caagtacggt gaacacatta taataaatgt tggcaaaccg atagaagttt
    29461 gcgaatacat gaatgaatat gaagaaaacc agcctgctgc cattaatcag cttcgcgaca
    29521 gactgcgcga cggtttgagt gaagttacat tggatattgc caccgaaaag aactacgaat
    29581 gctttgaaac ggccaccata gcagcaagtg cagcaatggt tgaaaagatg aaacttccca
    29641 atacagaaaa atatcgtttt cttgcccgcc aaaaaacagc tcgccaattg cttgaaatgg
    29701 aaaagaacaa accggagact atcgacaaat tgaatgtaat ttgcaaggaa tacagggaaa
    29761 acttgcgcaa gttgaacgta ccatcctggt tgctggataa aaatttgaaa gtttcttctc
    29821 ttttactcga aagtttattg ctcgtaatca ctttcccatt ttttctcatt ggtctcgttt
    29881 tgaatgcatt gccatttttt tctcccgaca ttatcatgaa ggcttttcat atcaattttc
    29941 ccggtggaaa aggctccttg cgttttggat taggcatggt aacctttcct ctgttttaca
    30001 ttttgcaatc ggtattgttc tacaaaaagg tgaaggattc actttgggca gtggctgtat
    30061 ttttaccctt gcaatattat ctgggaaaat gtgcttttct atggcataaa aaattcagaa
    30121 aacttaaagt taaatttcgt taccataaat tgcaagtaaa aaattcgctg atactgcaac
    30181 gtaccaaatt gctgcatgag gaaattcttc aattggtgca atcctaaaaa agagcagcga
    30241 aattctacca ttttaacttt tttgattttg aaaaaaaacg gaagaaaaat cattttctcc
    30301 cgtttttgtc ttttcgtata taaaaatttt taatcaagat tcagactgac tctcttaaaa
    30361 tcgattatag ttgcatgatg agctttcaga tattctccga ttgtaatttt actgtcttta
    30421 acggatgttt gttccaataa aaccgattcc tgaaaaaatt tgttgagtct gccttcagca
    30481 attttgtcga taatattttc aggtttgcct tcttcacgag ctttttcgcg ccctatttcg
    30541 agttctgttt caatgatatg agccggaacc gaagcacggt cgatagctat tggattcatt
    30601 gctgctattt gcatggcaat gtcgcgtgca acctgatgat ctacattttc ttctttgaag
    30661 gcaacaatag ttgccagctt atttccttga tgaatataag cggctgttgt accgcccttt
    30721 acaaaattat agtcatccaa ttcaactttt tcgccggtaa tagccgattc gtccacaacc
    30781 aagtcggcaa tggtacgacc gttaatagta aaagctttta attcatcaag tgttgccggt
    30841 tttttatcca ttgccacatc cagtatggat tgagttaaag ccataaagtc agcatttttg
    30901 gcaacgaagt cggtttcgca tttcagagca agtactgctg caaagccatc tttggctgcg
    30961 gcaagcacac aaccttctga tgcttctctt ccgctgcgct tggctgcgat tgcctgacct
    31021 ttttttctga taatttctat tgctttttcc agatcgttgt ttgcttctat cagcgcattc
    31081 ttacaatcca taagacctgc accagtcata gtacgaagtt ttgtaatttc ttgcaatgtt
    31141 actgccataa ttttttgatt taaattctga aaaatatctt caatttcatg catacatgaa
    31201 aaaaaagatg ttttaattgt ttataaatgt tgttgtaact aaaacgagct accagctaca
    31261 aatgaatcat aaccgtaact cgttattata gatcaagact gtaaataata atacttttga
    31321 ccgaatttat taaattaaaa tttttctcag tcaaatgttc tcattattta attttcttct
    31381 tcttttataa atttgttaac cacgttggct ttaagagcct catcgtcttc acgttgtgtt
    31441 ctgggtcgtt tatttaccct ggactttctt tctttcacta caggtgcttc tgcagcctca
    31501 gtctcaactt tttcgagttt tcgctcttca attccttcct gaatagcatc aattagagca
    31561 ttcaaaataa cttcaatgga tttggttgca tcatcattgg ccggaataac aaaatcgata
    31621 ttactggggt cagaattagt atccacaatc ccaaaaatag gaattccaag tttttcggct
    31681 tccttaaccg caatatgttc tttcattaca tcaaccacaa aaatggctgc aggcaaacgt
    31741 gttaaatcgg taatagaacc aagatttttt tccaattttt ctctttcgcg tgtaatttgc
    31801 agtttttctc gtttcgaaat attgtcaaat gtaccatcag tcatcatttt gtctatggtc
    31861 gacattttct tgatagcttt acgtatggta ggaaagttgg tgagcatgcc acccggccag
    31921 cgttccgtta tataaggcat tcctacagct tttgcttttt cggctactaa atcctttgct
    31981 tgctttttgg tagctacaaa tagtattttt cttcccgatt ttgctatttg cttcattgct
    32041 tgagcagctt catcaatttt tgcagctgtt ttgtgtaaat cgataatgtg aataccattt
    32101 cgttccatga aaatgtatgg agccattgca ggattccatt ttctttttaa atggccaaaa
    32161 tgacaaccag cttctaataa ttcttcaaaa tttgttcttg acattttact ttttttaatt
    32221 tgtttacttt ctgtgaaatt caattgttga gtagtcttta tttttaaaag agtctccaca
    32281 atttagatac taaaccaaat tcaattcgct gtttgtgtga ctttttgtta caaatatcat
    32341 ttgataataa aaagagaaac aacagattga tattatcgtt ttgagaactg aaatcttttg
    32401 cgtgctttgg gacgacccgg tttttttctt tctacaattc tcgggtcgcg agttaagaaa
    32461 ccttccgatt tcaaagcagg tttatcttcg ggatttattt ttactaaagc acgagcaatg
    32521 gctaaacgta aagcttcagc ttgtcctttg aatccgcctc ccttcaaatt aaccttgata
    32581 tcatatttct cagtcacatt caacttgtcc aaaggttgtt ttacaatgta ttgcaaaaga
    32641 agcgatggga aataaacgga caattctcgt ttgttgatta tgatttttcc tgttccttcg
    32701 ttaacaaaaa cacgagcaac agctgctttt cttcttccta aggcatttac tatttccata
    32761 ctgattattt aagtgagttt aaatcgattt gctttggctg ttgagcctgc atattgtgtt
    32821 ctgcattggg gaatacgtaa agatttttta aaagttttgc tcccaatctg tttttgggca
    32881 acataccttt tattacctta cggataagtc tttcaggatc ttttgccaac atctgggcag
    32941 gggtggtttc tctttgccct ccgggataac cggtatgtcg taagtaaata cgatctgtcc
    33001 atttgttgcc tgtcaatttt attttgctgg cgttgataat aatcacatta tcaccacaat
    33061 ctacatgtgg tgtgaaattt ggtttatatt ttccacgcag aagcttggcc acttttgttc
    33121 ccatgcgacc caatatctca tcacttgcat ctatcaaaac ccattctttc tgcacggtgt
    33181 ttttattggc agaaatggtt ttataactta aagtatccac ttttactaat ggtttaaata
    33241 aattaataaa ttctttttac ttaggtacct tttatcttat ttttggaatg gctaatcaat
    33301 acaaaataaa gacaataaat tataccaatg aactttttat tttggataat aaacggactg
    33361 caaaaatact ctttttcttt cgatttacaa atagttagag gtaaaatttt ttttaaaatg
    33421 atttttatgt ttttatttca actcgggaaa ttttttcgat atttgctttt gtgtttttcg
    33481 gagcgtagaa aaatttgact gataatcgtc tttaatcaat aattttttaa ttttgtggat
    33541 gaatcgaaat catcataaag ttggtattaa attatgcata aatcgggttt tataaattta
    33601 gtcggtaatc ccaacgtagg taaatctacg ctgatgaatg cccttgttgg ggagaaactt
    33661 tctatcatca cctcaaaatc gcaaacaacc cgtcatcgaa ttttgggaat agtcaacgag
    33721 gaaaattatc aaatgatttt ttcggacacg ccgggcattc ttaaaccaaa atatgctttg
    33781 caggaatcca tgttgcaatt ttcacgttct gctctcaccg atgccgatat tctgatgtat
    33841 gttactgatg tgtatgatgc agaaccaaaa gatatggatt ttgttcaaaa aattaattct
    33901 gataatgttc ctttattggt tttaatcaac aagatagatc ttattaatca ggaaaaattg
    33961 gaattgttgg ttgaaaaatg gcatggcatt tttcctcgag gtgaaatact gcccatttct
    34021 gcactcgaaa agttcaacgt ggattacctt atgaagcgaa tcaaagaact attgcccgaa
    34081 tctcctcctt tttttgacaa agaccagtta accgacaaac ctgtacgttt ttttattagt
    34141 gaaatcattc gcgaaaaagt gttgttgaat tatcagaagg aggtgcctta tgccgttgaa
    34201 gtggagatag aagaatttca tgaagaggaa aatcttgtca gaataggtgc tgtgatttat
    34261 gttgaaagag attcccaaaa aggaatcatt attggtaagg gtggaaaatc gttgaaaaag
    34321 gttggtgtac aggccagaaa agatatagaa gaatttctcg gacaaaaagt ttttcttagc
    34381 ctttatgtaa aagtcgaaaa agattggagg aataaggaga aaaagctcaa agactttggt
    34441 tatttgcacg attaatccta taattttgtt gaggtattgt tttaaaattt tctctttcga
    34501 tttaaaacaa atatttctta aaagtgttat ttgagtataa aggtgtaata aattgctttt
    34561 atgcttaaag atttacgcaa acagtacgac cgatctactc tgaatgaatc gaatatttta
    34621 aaaaatcctt ttcaacagtt taagttgtgg ctgagcgaag ccataaatac tgaggaatct
    34681 gaacccaatg caatggttct atctactgtt gatgcagaat ggcaacctca tggcagaatg
    34741 gttttattga aagaattgac tgacgaagga tttgttttct ttacaaacta taaagggaat
    34801 aagggattgc aaattcttca taatcaaaga gtttctctgt tgtttttctg gccggttttg
    34861 gagcgtcagg tacgcataac aggcaatgcc gaaaaaattg cagagcaagc ttccgacgaa
    34921 tattttaaaa tgcgtccttt attcagtcaa attggtgcat gggcatcacc tcaaagtcga
    34981 aaaattccgt caagagattt tcttgacaaa caattcgagt attataagca taagtttgaa
    35041 actgaggtgc cacgaccacc tttttggggc gtgtttctgg ttcgccccaa aacttttgaa
    35101 ttttggcaag gaaggcctga cagaatgcac gatcgattgt tctacacaaa aaacgatcaa
    35161 caatgggaaa tttcaagact tgcaccttaa gaacacagat tttatggcat aattattgta
    35221 aaaagttact gaaaaaaatc tttctttaga atcaaaaatt ttgctttaaa aactttcttc
    35281 gatttaaatt tttattgttg cgtgattttt ttgattatta taaataattt tttagaaaaa
    35341 aagttcgaaa attattttga ttttgaaaaa aaataaatat atttgccgat atttctatta
    35401 aaaattattt gcattaaagt aattttatta attttgcggt gaatgccgat gcttgaatta
    35461 taaattagag gcaggaaata aatttttaag ttttattgag gtgtgttttc aataaatcct
    35521 atttatcttt tttaatcaaa ataatattcg gaaaatcaat taactaaaaa agtagaatta
    35581 tgaaaaaaat cttattgagc gttatgttat tggcagttgc tgtaacttac gcatctgctc
    35641 aaagcacggc agaaactaaa gcgaaagccg atattgctca ttggtcggcc ggtattaagg
    35701 ctggagtgga ttactatcgg gttacgcctt ccagtactgt tacagattat cttttttttg
    35761 aaaattcttg ggcgcgttat gccaacaatg cagcatggac tgctccacaa atttatgtag
    35821 aatacacttt taatccactt tttggattgg gtgccgattt cagttatctg acttatggca
    35881 gaaatacggg tgcgggtcat acgcttgatt ttaccttgta tggttctaca aacttaagta
    35941 atttactggc tccaaaacgt gctggctttt ggggtaaagt aaatatttat ggaaatctgg
    36001 gcgcagggtt gggattctac tcttattcag gtgttgcagg agttactgat ggtgataatt
    36061 tgaaatctcc acttgcattt gccgctctaa atgccgaata tcgtctcagc aaagtattgt
    36121 cgttgggagt ggaaggtcag taccgttatt atatgagaga aaatttaggc gggttgagta
    36181 acaattcttt aggtagtgat gctttgatcg gtacaattgg actgcgttat aaatttggcg
    36241 gagctacaaa atctcatgtt cgcgacatgt ctttggaaga atattatccg gctccggcac
    36301 cggttgttat cgaaaaaact gttcaagatg gaaaagttga cgccaatacg gaaaatcgtt
    36361 tgaaagcttt ggaaaatgac aatgccatga taaagaacaa attgcaacaa cttgaaagcg
    36421 atttaaaggc tttatcgcag aaatcaaccg gtactgttaa tgcaactttc cagaatattg
    36481 aatttgaatt tggttcttcc caattaacca agcaagctta tgatttgctg gatcagattt
    36541 atgctatttt gaacagtaat tctactgctt ggacaaaaat tatcgtttcg ggtcatactg
    36601 acaatattgg accaaaatcg gttaatcaac agttgtcgat tgctcgtgct caagctgtaa
    36661 aggattatct tgttaaaaag ggtattcctg cttctgccat tactgttgcc ggttacggtg
    36721 aagataaacc tattgcttca aatgatactc ctgcaggtag acagaaaaat cgtcgcgtag
    36781 aatttgaaat ttcaaagtaa aaataaatag ttgatttaaa attaaagggc gactccaagt
    36841 ttgaagtcgc cctttttaaa ttgaaaaaaa attgtggcag ctcaaataat gccacttttt
    36901 aacaaaatat tttccattgc ctgttgaagt tgcaatgctt caaagcgagc tgcttcggca
    36961 aagttttcat ccgaagaggc atagataatt tgacgcgatg aatttaccaa caaaccacac
    37021 tgactggtca atccaaattt tgccgtttct tcaagacttc ctccctgcgc cccaataccg
    37081 ggaattaaaa gaaaatgttc aggaacgatt tctcttaccg attttagata ttctgttttt
    37141 gttgcgccaa cgacatacat tatattttct tccgttcccc aatttgtaga tgttttcaaa
    37201 actttttcaa acaatctttc tctggtattt tcttccttta tcagttgaaa gtcgaaagct
    37261 cctttattgg aagttagcgc aagaataatg gtccatttat tcttatagga aagaaaaggt
    37321 ttaatggaat cttcacccat atacggagaa acggttatgg cgtcgaaatt cattttttca
    37381 aaaaaagctt gcgcatacat ggccgaagtg ttgccgatat cgccacgttt tgcatcggca
    37441 atcaaaaatt gatcgggatg atgattccta atgtaagcaa ctgtttcatt cagagctttc
    37501 aaaccttcta tcccgagact ttcgtaaaaa gccagattgg gtttgtacgc aacgcataaa
    37561 tctgccgtcg catcgactat aactttatta aaatcaaata ttggatttga tgatttttct
    37621 tttaaataaa taggaatttt atttatatcg gtatccaatc cgatacatag aaatgatttt
    37681 ttccttttaa tattttcaaa aagttctttt cggttcattt tgttggaatt tatcaatatc
    37741 ccatatgtta taattctgca tcttttaatc tttcagcatt ttctgcaatc ataagctgat
    37801 caatcattgt ctgaatttcc ccattcataa atgcgggcag gttgtaaata gtgtaattga
    37861 tgcgatgatc ggtaactctt ccttgcggat aattataggt acgaattttg gccgatcgat
    37921 cgccggtaga taccattgtt ttacgtcttg aaccgatttc gtcaagatat ttttgatgtt
    37981 caatggcata aattcttgaa cgaagttcaa tcatggcttt tgccttgttt ttgtgttgcg
    38041 acttttcatc ctgacattgc acggtgattc ctgtaggaat atgtatcaac cgaacggcag
    38101 aataagtggt attgaccgat tgtccgccgg gtcctgaaga acaataaata tctacgcgaa
    38161 tgtcggattc ttttaaatcc acatcaaact cttcagcttc cggaaacacg gccaccgtcg
    38221 ccgccgaagt gtgaattcgt ccctgtgttt ccgtttgagg aactctttga acgcgatgta
    38281 caccggattc gtatttcaat gtgccataga cattttcccc ggatactgta aaaattattt
    38341 ccttgtaacc tcctgccgta ccttcattat aatcggtaac ttcaattttc caacctttgc
    38401 tttcgcaaaa tttggcatac atgtgaaaaa ggtcgcctgc aaaaattgct gcttcatctc
    38461 caccagttcc gcctcttatt tcgacaatgg catttttcct gtcttccgga tccgccggaa
    38521 ttaataaaaa tttcagcttg ttttccaatt ccggaatttt ttgctccagt tcttcgattt
    38581 ccgaccttgc caattcacgc atttctgtat cactttcggt ttccaccatt tgttttgctt
    38641 cagtcaagtg cgataatgcc aattcatatt cgttttttac gtttaaaatt tcgcgtaaat
    38701 cgtgatattc cttgttgagc ttggcaaaac gtttttgatc ggaaataaca ttcggatcgg
    38761 tgattaatgt cgaaatttcg tcaaatttat tttgtagtcc ttccagtttt tccagtaaag
    38821 ttttttcggc cattttaaac gatttatcgt caggtaatat cttgacgtta attttaattc
    38881 ttttgaatgg aaattgagct aaatttttaa taaaaaattc aaatgatttc ggcaaaatta
    38941 gtcaaaatta aaatttcttt caattttcct caacaacctt tttaaatatt ttttgtgcat
    39001 ccttatacgt atttatcgtg caaggaccag aaatgcagcc accttcgcaa gccattactt
    39061 ctataaaatt tcccggtgct ttccctttgg cgtatgcttt gagcagagca atatttttct
    39121 tgtcaatatt agtaactatc aacggcttta ttcctacatt tgggtaagac tccataaccg
    39181 cagcaattac tccgccggat tgagcaaatc ctctaccagc ttttatttct gcatcatcct
    39241 tattttcagt tggtatgatt tctattttca ttccctttaa aatagcatcc aattcttcaa
    39301 aagtcaatac gtaatcaatc gtttgatcat cggctgcctc ttttcttttt ccaacacagg
    39361 gaccgatgaa acatactttt gaatcgggat atttattttt cgctatttta gaggcataat
    39421 acatgggtga attggtttga gaaacgaatg gcttcatttc aatcagatgt ttcttggtaa
    39481 gttcaacgta agagggacag caggaagtag tcataaattg ctgaccctgc tccaattttt
    39541 gctgcaattc ttctccttca tggaaagctg tttccattgc ccccagcgct acttctatga
    39601 tttcagaaaa tcctatattt tttatagcgg ttgctatatc cgaaaaagaa tgttcaaatt
    39661 gagactgaac ggaaggagcc acaatagctg taattttttc atttctttgg attgccgata
    39721 aaacatcaaa tatttgagaa atttcaaaaa tagagccaaa tgggcaggca ttgatgcatt
    39781 ttccgcaaaa aatacatttt gattcatcaa tatgttcaat cccgttttca tctttttgta
    39841 tggccttcac gggacatgat tcttcgcacg gaacaggaac gaaaactatg gagtgataag
    39901 gacatgcttc tttacaaatt ccgcaattta tacatttatt atgatcaatt ccagcctgac
    39961 cgttttcttt aaaaaaaacg gcttctcttg ggcaattcat gtaacaggct cgtgctacgc
    40021 aacctttaca caaattggta actacgtaat tggtctttac acatgctgtg caggcttcat
    40081 ccacaacagt aaatatcgat ttatttttct ctgttctcag aagtgctttc cttgcatatt
    40141 cagaaagcgg agtgagttca tcttcttcct cttctatgct gtatccaaga attggcatta
    40201 tcttgtactt taacaccgct ctttccttgt gaatgcaaca tcgtcctcgt gcttgtgctt
    40261 tcttaggcga cattttaaga ggaacccgat cgatttcact taaaagtaca ccttcattga
    40321 acaagcttac caattttgtc attaaatctc ttcttactat caatgtattg tttaaaaaag
    40381 ccatatttcc ttgtatttga gtggaatttt gcttgaaagt tttattctta aaccttactt
    40441 ttttgtttgc aaagttaggg attccttttg aattaaagaa atttttcctt aattaagtat
    40501 gtcgattttc tgaaaacata aggatagttt tttacgaaga aatcctgaaa ttatgcaaga
    40561 agaggaaaga accagtattt gagaacaata gtaggcatag tggagaaatc caacgaaatc
    40621 tttaaaaaag atccacccat gaaaagaatt ttgaaattca aaatttactt cattaaagaa
    40681 agcttttcac atcaaccaaa ttttcaatcc caatcaattt ttccactttt tatgagaatc
    40741 gaaaaatatc atgtaaattt gaaccatatt ttttcaagat aattattatt catgttttct
    40801 tttaataaaa tatatcggtt atcccagcgc ctaaggctcg tatttattgg aagcgctgca
    40861 ttgatagtaa cggcatccct atttttcacc aacagactca ctcgtcaact ggcaacagaa
    40921 gagcacaaaa aaatggaaat ttgggcaggt gccatgcgac aggtgatctt ggccggagaa
    40981 aataccgata taaattttct ctcggaaata atttcgggaa acacaaccat tcccgttatt
    41041 atgaccgatg aaaatgataa cttaatttct tctcttaacg taaatgaacc tgcttcaaaa
    41101 accaatgact attatcggag agaaattgaa cggctaaaga aaaaaaattc acatattgaa
    41161 gtaagaattg acaacacgtt acaacatatt tattatgacg attctttatt actaaaacaa
    41221 ttaagatatt ttccgtacat tcagttaggt gtaattttta tttttttgct tatagcctat
    41281 ctggcttttt cgggaacaaa aaaggccgaa caaaatcaag tatgggtagg actttcgaaa
    41341 gagacagctc accaattggg aaccccaatc tcttctctgc ttgcttggct ggatttactc
    41401 cgaatacgca atgatgacaa attgctgaca gaaatggaaa aagacgtcaa cagactgaaa
    41461 attattgcag aacgattttc aaaaattggc tcagctcctg atcttcaact agtaaaactg
    41521 aatgaaacga tcacaaatgc agtacaatac ataagccatc gttcatcgca gaaagtaata
    41581 atcacatgcc attttcaaac ttctgataat gacctgattc ggatgaacgt tccgcttttc
    41641 gaatgggtaa ttgaaaatct ctgcaaaaat gccattgatg caatgaatgg aaatggaaaa
    41701 attgacattt tcgtccaaaa aaaatctaac gaatacattg ttgacgtcaa agacacagga
    41761 aaaggaatag aaaaaaataa atttaaagcc gttttctcca ctggatatac aaccaaacca
    41821 aggggttggg ggctcggact ttcgctggcc aaaagaatta ttgaggaata ccacaaggga
    41881 aaaatttttg tcaagcagtc cgaaatcgac aaaggaacca cttttcggat tttgatgaaa
    41941 gcttgaaaaa aagcaacaaa ccatgtttat ataaagtgtt tgcttataaa attgataaaa
    42001 taaacggaaa aaatttcaaa ataatataga attttgtacc tttgtaccgc tttttaaagt
    42061 tattgtgtca tgtcgaaatt tgagctttac aacattgtac tgaaagacct taccaatgaa
    42121 aaacgcattg tagaatttga tttggacgat gcttattttc agaaaatcga tagccctgaa
    42181 gtacaacaag gcaacgtaca tgcaaaaata accgtacaaa aaaaaactac ggtttataaa
    42241 ctcgatttcg aactgaatgg ttttataact ttgccttgct cccgttgctt ggaagatatg
    42301 caacaactca ttcattacaa ggagtcctta gaagtaaagt ttggcaacaa attttcggag
    42361 gaaaacgata tagttatcgt acctgaagct gatggagcaa taaatgtggc ttggtttctt
    42421 tacgaattta tcgtactgaa cattccattg aaacatgtac atgcaccggg agaatgtaac
    42481 aaagcaatga taaccaaact aaaaaagcac atttcatcca caaaggaata cgaggatgaa
    42541 acttctttgg aacttgaaga taccaacgaa gacgattatt tttcaaacaa tcagttcatt
    42601 gatttagaat ttgacaactt acagaatatt gacaattaaa taaaaaaaaa taaaataaaa
    42661 taaaatggca catcctaaga gaaaacactc gaatcagaga accgcaaaaa gaagaaccca
    42721 cgataaggcc aaactcccaa cgctggcaat ttgttccaac tgtggcgcaa cgtatattta
    42781 ccacaccgtt tgtggcgaat gtggttatta tagaggaaaa ctggctatcg aaaaactggc
    42841 taatgcttaa ataaagcatt gaattttgaa aaaattttta aaatcgtaat gtataaaaca
    42901 attgggccct gaggaagaaa ttcagctttg ggcttgattt tttattaata tatttacaat
    42961 atgtctaaaa ttaatgcagt catcaccggt gtgggaggat atgttcccga ttatgttctt
    43021 accaatgaag aactaagcac catgatggat accaatgacg aatggatcac cactcgtgtt
    43081 ggcatcaagg aacgtcgcat tctgaaggat aaggaacttg gaacatcctt catggcctcc
    43141 aaagctgtcg aagatttgtt gtccaaatca ggtacgaatc cagatgaaat tgatatggta
    43201 ctatgcgcta ctactacacc cgattacata tttccggcat gtgcagcaat agtaactcaa
    43261 aatgttggaa ttaaaaatgc ggtcgcattt gatattcaag ctgcctgctc ggggtttatc
    43321 gtgggtttga ccacagccac aggtttaata gaatcgggca gatttaaaaa aatactggtt
    43381 attggagccg aaaaaatgtc atccataacc aattatgaag accgcactac tgctcctctt
    43441 tttggagatg ctgcaggagc tgttttggtt gaacctacaa ccgaaaacgt cggaatcatt
    43501 gattctatct tatttaccga tggtgttggt atctctcatc ttaatttaaa gggtggaggc
    43561 tcagtttatc ctgcttctca cgaaactgtt gacaagcgca tgcactacat ctatcaggaa
    43621 ggtcagtttg ttttcaaaca tgcggtttcc gatatgtcgg atgctacagt gaaaattctt
    43681 gaaagaaatc atctgacaaa agacgatatt gcatggctaa ttccccacca agccaattta
    43741 cgcattatag atgctgtggc tcaacgtatt ggtctggagt atgaaaaagt aatcatcaac
    43801 atcagtcgat acgggaacac ttctgctgct acaattcctt tatgtatgtg ggaatcggag
    43861 aaaaaattta aaaaaggcga caatataatt ttaacagcct ttggtgcggg ctttacatgg
    43921 ggcgccgtat atctgaaatg gggttataca aaggaatgaa tttagtgttt ctatataaat
    43981 aatcattcag gcaggctttt cgcctgcttt ttttttcttc atttctacaa aaaacataac
    44041 caaaattcat aaatggagaa aaaatagttt tgaggtaatt ctaatgcgga gtgataaaaa
    44101 aagtcaatga attttcatca gaatataatt tattttttaa aggtctgatg gaacccacat
    44161 gtctgaatat tatattcatt cgtctttgtg tatgcaatac atgtggtttt catagatcct
    44221 ttgtaagcaa agaaacaact gcatggaaat aatgaattca acgtcattca tgagacttat
    44281 aaatgtaagc tatgttactg ttaggaattt tattcggata tacatattaa ccattatttc
    44341 caacggtacg ataattttcc attgaacaat ccttccttta tttttggtga gcagcctgta
    44401 aaaatcattt tttttatgta agtttgcatg aaaaagaaga aaaacaactg gaattgcaac
    44461 aaaatattta ttccaaaatg tttttataat tgtcttaaaa tcaataacat tgtgtttatt
    44521 tttacaacag tatgaaaaat tacgttgttt cggcgcgcaa atacaggcct acaactttca
    44581 aatcggtagt tggacagact gctctgacca atactttgaa aaatgccata aaaagtaatc
    44641 atttggctca cgcttatctt ttttgtggta cacgaggagt aggaaaaaca acttgtgctc
    44701 gcatttttgc aaaaaccatc aattgtgagc accttaccga agactttgag gcttgcaacg
    44761 agtgcgagtc atgcaaagct tttaatgagc aacgttccta taacattcat gaattggatg
    44821 ctgcttccaa caatagcgtg gaaggcattc gttcattgat tgatcaggtt cgtattccac
    44881 cgcaaatagg aaaatacagt gtgtatatta tcgacgaagt acacatgttg agttccagtg
    44941 ccttcaatgc ttttttaaaa acactggaag aacctccggc tcatgccatt tttattttgg
    45001 caaccaccga aaagcataaa attattccaa ccattctttc gcgctgccaa atttatgatt
    45061 ttaatagaat ttccgtatca gatattgtgg gacatctgca atatgttgct catgaagaag
    45121 gaataacggt agatgtgaac gggctaaatg tcatagctca aaaatgtgat ggaggaatgc
    45181 gtgatgcgct ctctattttc gatcagctgg taagcttgtg tggaaataat atttcttatc
    45241 aggatgtgct caccaatctg aatgttttgg actatgatta ttattttcag ttgatagaag
    45301 cctttatgaa aaatgatgtg aacacttctc ttcttatttt caatgatata attcaacacg
    45361 gttttgatgc acatcatttt gtctccggac tgagttctca ttttcgggat ttgttggtta
    45421 gtaaagatcc tcaaaccatt caattgctgg aagtgggtgc cgatatagga aaacattatc
    45481 aaatgcaagc tcagcaatgt cctgaagatt tcatatttaa agccttgaaa atttgtaacg
    45541 attgcgattt gaattacaat atcagcaaaa acaagcgatt gttggtagag attatgctga
    45601 ttcgtctttg tcaacttgtc aaacaggagg ataaactcaa tggggaagag aaaaatgaac
    45661 ctctgaaaaa agtggttgat gagctgaaaa caaataatgt tgttctcgct aaaaatagcc
    45721 cttcatcgac taaagttcaa gattcaacgt caaatccaat gagtacagat gttgacgcaa
    45781 agcaggtgaa aaccgaacgt ccgacaagta tttccattaa aaacaatgtt gtttcttctc
    45841 cttcatcgtc ctatgaggca ccacaaaagg agcagcctga tattcggaac gaaattttta
    45901 ctcaggatga cttgcaaaaa gcttgggctg aacttgctaa aactttagta gatgaggcac
    45961 gaatgaccgc tttcatgcag tcaaatcgtc ccgagcttat ttccaatact gaatttagaa
    46021 tattagttac caatgagtta caagaaaagg aattaaaacg gctgaagccg actattttga
    46081 attttttaaa gaccgcttta aaaaattcaa acataaaaat gtctattcaa attcaagaag
    46141 ataggtcggt acaaaaagga agaactcctg aagagaaata taaaataatg gcagaagaaa
    46201 atccggcttt agcaaaactt agaaatgatt tgcaactcga aattgattag cagccggcaa
    46261 aaaaaatctg aaatgttaaa ttgattcaat aaaccaggtt cgacataaaa atgcatcatt
    46321 cagtacacca agaaaaaaat taaatttcat cagaatataa tttatttttt aatggtccga
    46381 tggaacccac atgtctgaat attatattca ttcgtgtttg tgtatgtaat acatgtgggt
    46441 ttcttaaaac cagataaatt tttataacga aatatgtata ataacaggtt gtacttgtat
    46501 cttgaattca gcaattaata aataagcagg taaattttta ttgcctgctt atttaatttt
    46561 tttttatact ttcgcacatt tttttctacg ttattccttt ataattttgt gtgcgctttt
    46621 gccgtttgcc gtttccagtt caaccaggta aaatcctttt ttcagcattg aaagatcaat
    46681 ttcttttata tcattgtttt gctgtttaag caacgaaccc tgcaagttat aaatgcgaat
    46741 gctttggata tttgcggtag aggaaatgaa caatttcccg cttgtcaaag ttggataaac
    46801 ataatcgctc acattatagg atatttctct gttgtcatta gtaaaattta catttcggtc
    46861 agtgaaaatc agtagttggc cgggctgcat gtttatagtc atgttggtat tgctcacatt
    46921 cagggtttcg cctgtcagca aattgtacca tgtgccggtg cgttgaaagg acgggttggc
    46981 tgttattgaa gcatcagatt gaaaattacc caaaactacc atattcaggt cagcatgaga
    47041 gagagctatt cttctcccct gcgaccagtc gttataggcg acttgcaaag aataatttcc
    47101 gttcagaaag gcattaggat acaatttacg catagatata atttttgcac aggcgtcggc
    47161 agccgctttt cgatgcggca aattcagcca gccccaagcg tgaggttttg ggttggttcg
    47221 gcctccgaac gaatcgatgg aataatcgta acccatttct tcaaattgcc acaacatttt
    47281 tggaccaggc atcaaagttg cgaacgccac taccaaaggt acacgtgaaa ttctgtataa
    47341 agaatcagtc ttaactgtgc cattcccata agtttttgct ttatagaaat ttctttcttc
    47401 atcatgactt tcagcatagc ccacccatct gcgcggagaa gtattcatgc cagaaaaatc
    47461 actattgctc tgaaaaccca ttgcagcctg cgaatatgcg ttattcaaat ttcgccacaa
    47521 atacataccc tgagctgcca gataatcttc ttcgcttcct cccacaaaat gttcgagaat
    47581 aaatagggca tctggtttca cgtcggttac tgcgtaataa taatccttca gattgttgat
    47641 acgggtttgg tcccatgtcg attccgttcc cgagttttgc gtaaatccct tggtcaaatc
    47701 catccggtag ccatctactt tgtattcagt aatccaatac tgcagcactc ttttgaaata
    47761 ttctttggtg ccggaataac tatgattgaa atcgttcaat acactgaatt gatgaggtgc
    47821 cacgggattc atccatggat tatcggcagc agggcgattt tttgtgctgt cccaataaag
    47881 cagtgcaaac ggacaaattc ccgaagcctg attgaaaacc atatcgagaa ttacagccat
    47941 acctcttttg tggcattcgt cgataaattt tttgtacatt tcgggtgttc cgtaagcttt
    48001 atccggcgca aaataatgat taggattata tccccaactg ttgttcccat caaattcctg
    48061 tataggcatt aattccacag ccgtaattcc gagatttttc agataatcga gtttgccgat
    48121 ggcagcttga agcgattttt ctgtagtgaa atcgcgcagc agcatttcgt aaatcaccag
    48181 attttctttt gccggcatgg aaaaattggt aatttcccaa ttataggcag gtttcgcagt
    48241 ttgaaaagtt gcaaccagac cttcagtttt accttcggga taaggtttca gatttggata
    48301 aatggtgtaa tactgattga tccatttgtc gttccacgga tccagcacga gttcagtata
    48361 aggatcgctg cagcgaatcg ttccatcaac cagatattga taggcgtaaa tttttcccgg
    48421 agtcagtccg gttaaggtaa tccaccagta ttcaccatct tttttcaatt gataggcatt
    48481 cagttgtgcc cagtcattaa attctccaat aagaaaaaca ttggttttat tgggtgcatg
    48541 aaaaaccaaa gttacagttg tgttgtcgat gtaattgatt cccagtttag tccctgccgg
    48601 acgagtttga ttcacaaccg gttcgggaac gcaaaccagc agggtatcgt aaacggtatt
    48661 gttgtcgact ttggcagaag caataaaagt ataatcgcct gccacactaa aattgtagga
    48721 ataggttagt gaggtggctg cctgttgtgt agccagaacg gtgttgttga gcaacaattt
    48781 aagttcggca ctcatcgaag aactaactgt aaagtcgata ctagagccga tagcgacact
    48841 ctgattggag gttgggaaag taaaagccac attaaaattg ctttcataaa cagttacaaa
    48901 gatatccgct cctcctatat cttttccctg tagggtacca gtgctatttc gaaaaacaaa
    48961 agccaacttt ctgacaactt ctccgggact taaattataa tatcctgcca tattgggggt
    49021 tatcaaaagc ttccatttgt tattgcccag cgaagtcaac ttatattttg gagaattgtc
    49081 accccaaaca ggtgcatgtt tccaatcgct cggtgaagtg cttgatgagg tgattacgcc
    49141 tgtgtgagca tatacatcgc cggtgtaatc tttcaaacct gcagtgcctt gtgtggcgtc
    49201 ataaataatt tctatttctc ccgggtagtc tttggtgata aaagtgggat tggtagtaat
    49261 gatttgtccc cagcttaata aatagcaaag cagtaatgaa aaagtgaaaa gaattttttt
    49321 catgataata gcatttaaac agtttgactt acatgcaaat gtaaaagttt agtttctaaa
    49381 acatcgaatt ctctataaaa attaaaagat tttttcgtgc aaacgattgc aaaaagaata
    49441 cagtattttt atttgccttt attgtaaata actaaagttt cgcagttctt tatttcctgt
    49501 ctttcaaatc attaaagcga agtgcgaaac tttagtaaat aatctttcgt aagttaatgg
    49561 tgtacatcca agagcaaccg tttaacattg ccaatggcaa tgtacagcca gggtaatatc
    49621 tttggcatgt cgtctttctt tataacctgt gcatgatgtg atttgacatt ttttgtcaaa
    49681 ttgttgtaag gagtagcatc atcggtgatc cattcaacca ttggatttat gtgttctttg
    49741 acagtgttta aaatgacgtc agccttttaa tttaggatat tttgcgaatt atcattttat
    49801 attatttcga ccgcgctagc ttaatgtatc tttatacact atttgtcaaa agtcaagata
    49861 gccttattat cattaaattg aaaaagtatc atattctgtc aaaaaatgat atgaattatc
    49921 cactggataa tgttattttt gtcttcactt tataatattg agtagtgtta ctttaaaaaa
    49981 tctaatatca tgaaattttc ttttttgtca tcactctctt taattatcgt ttctgccaat
    50041 atttgtttgt ctggttttgc acagtgcggg aaaggcgaat atacaatctc agcttttccg
    50101 tgtttaaact gtgctgtaac ctgtcaaaaa cctgcaggcc cagaatggat acacaatgct
    50161 acattttatc aaatttatcc acaatcgttt aaagatacaa atggcgatgg tattggcgat
    50221 ttgaaaggtg taatcagtca gttggattat gtaaaaagct tgggtgtaga tgctatctgg
    50281 attaatccgt tttatgtatc gccattcaga gatgcaggtt acgatgtcgc cgattattac
    50341 catgttgacc cacgctacgg taccgatcag gatgccaaag atttattccg gattgccaaa
    50401 caaaaaggac ttcgaatcat tgtggatttt gtgccaggac atacctccat tgatcacccg
    50461 tggtttaagg cctcgtgcag ttcgacgcca acaaaataca gcaactggta tatatggaca
    50521 aatggaacct ggttcgatga cgaaaacggc aagtataaag cgaattttat tcagggcttt
    50581 tgcgacaggg atggcaatta catgattaac tttttctggc atcagccagc attaaattat
    50641 ggttacggtc agccagatcc gaaacaaccc tggcaattaa gcaccactca tcccgatgtc
    50701 gttgcactac gtaccgaaat gaaaaatgtt atgcgttatt ggcttgaaat gggctgcgat
    50761 ggattcaggg ttgatatggc tggttcgctg gtaaaaaacg accccgaagg aagtattaag
    50821 tatttttggc aggacattcg aaaaatgctt gatcaggaat atcccgatgc attcatggtg
    50881 tcagagtgga gtgaatcagc tactgctgtc aatgccggtt ttcatgccga ttttatgctt
    50941 tggcaggaag gttatcagga cttgtttatg cacgaaaata gttatttcag gtctgcggga
    51001 atcggaagta tcagtcattt tatgtggcga tacaacgaac aaagcactga aacaaatggc
    51061 aaaggatata tttcgttaac tgtaggaaat cacgatttgg aaagaataaa taacaggggg
    51121 cgcaacaggg ctgatttgga aatcattcag gtgtttaact ttacgatgcc taacattccg
    51181 tttatttatt acggagatga aattaccatg cgtcaactgc cgctcgttac aggaaaagaa
    51241 ggatgctacg gtaccagagc aggagctcgt acaccaatgc aatgggatac cacagcgaat
    51301 cttggctttt cgaccagtgc tcctgaaaga ctctatttgc cagtcgacag ttcaaggaat
    51361 gctcctaatg ttttagcgca agaaaatgac ccggattcat ttttaaattt tatgcggaaa
    51421 ttagtcctgt tacataaaac tgaaccagca ttgacaaatt atgcaaaatt tgttcccgta
    51481 tatgttcaaa aaaacaaata tcctttcatt ttcatgcgga tatttggtaa cgaaaaattg
    51541 ttggtaatac ttaatcccga tagtaaaccg gcaacagcaa ttgttgaagg aaacctgaaa
    51601 ggcaagcgaa aattattgct cggcaaactc ccggtttgga aacaaaatac aggaacaacc
    51661 gaaatacagg tagatggaca atcctattca gttataaaac tcaaataagt ctttttattg
    51721 atgaaaaagg tgagccgata ttattttatt ttctcagcgt tagccctttt attgctgttt
    51781 tcaggttgtc gtagttcgca aaaaaactca aaaaccagta aaacaggctt cactcctgag
    51841 gcttatacgg ctttgtatgc cgataagtgg tcggcaataa acgacagtgt gaaagccggt
    51901 tggaaaaatc acagcggaaa accatctaac aatgctgtgc ccgacacatt tgcgtatgca
    51961 tttaacgagt ggacattatt ttattgggat ttgtatttta cacaaatcgg attattgaaa
    52021 cataacgaac taaagctagc taaaggaggg ataaataacc tgaaagcgga actggacaag
    52081 cgtggattta tacccaatgc gaatacggat tggggggaca accgttcgca accaccctat
    52141 tttggcataa tggtaaaaga attttacgat tttacaaaag ataaaaaatg gttaaaaacg
    52201 atgtatgctg ccgttttgaa agaatacaaa ttctggacag atacatcggc gaaccgaata
    52261 gaggcaaata caactgaact tggtcttcag cgctattttc accatgccac cgaaacagag
    52321 ctacttgaac tgtatgatca ggaactcact tctcgttttg actttccaaa agatattgaa
    52381 aaatccaaaa aattgaatat tgcttcgcaa tttgctgctg aagctgaatc agggatggac
    52441 tttacgcccc gtttcgaaaa tcgctgttcg gagtttatag ctgtcgattt gaactccaat
    52501 ctatatttgt acgaaaagta tctggcttat tttgaacaag aacttaaaat aggtaatggt
    52561 cagcaatggt tagcaaaagc ccgaaaaaga aaagcgctta ttcagaagta ctgttggaac
    52621 gatgaacgtg ggttatttct ggattatgat tttgtaaaca acagaagcag taaagtagca
    52681 gctgcaacag cattttcacc gctctttgca ggtctggcct cgaaatatca ggcagaccga
    52741 atgcgtgaaa atctgcatct gttcgagagt gaaaacggaa ttgtagcctg cgaaagcacg
    52801 gatgaaaaga gaatttacca atgggatcat atttccgtct ggtcgccaat gcaatcattg
    52861 gttatcatgg cgcttgataa ttatggtttt aaggaagatg cctgccgcat tgccatgaaa
    52921 catctagacc tgataacagc caattttttt aacccaaatc catcaactta tgtagataaa
    52981 agcggaaaaa aattttacag attgccagga ggaatttatg agaaatacac ccgatttggc
    53041 acaataaacg acagggaata taatgcaagt ctaatgtatg gttggtcggc aggagctttt
    53101 gcttttgcat acgattattt aattgttaat agtcaacata attttactat aaaatgaaga
    53161 aaatcagaat tttatttata attacttttt tgttcatttc attgtttgga caaaaaattt
    53221 tatgtcagtc tgaattaaaa ataacttcgg accatcatta tttgagttat aaaaacgggg
    53281 acccattttt ctggcttgga gatacagctt gggaattatt tcatcgttta aaactggaag
    53341 agattgaaac atatttggaa aacagaaaac aaaaaggatt taatgttgtt caagtagttg
    53401 tacttcctga aatggatgga ttgagagttc caaacagata cggcgatgtt ccccttatag
    53461 atatggatcc tacaaaacct aatgaaaaat attttcagtt cgtcgatacg gttgtaaaat
    53521 tagcagcaaa aaaaggtatt tttatgggtt tgctacccac ttggggagat aaagttactc
    53581 agctctgggg tgaaggtccg gtggttttca atcccgaaaa cgcctataaa tatggaaaat
    53641 ggataggcga aaggtataag agttataaaa atattatatg gatacttggt ggcgaccgac
    53701 cacccatgaa ggatagtgcc gactggcgac ctgtttggag aaatatggcc aaaggaatag
    53761 aaaaagctac caatcattca gcacttattt cttatcatat ctggggaggt gaaatgtcaa
    53821 catcacaata tattcattct gagaaatggc tggatataaa tatgatgcag tcgggtcatg
    53881 gttcgggaaa agatgttcct gtgtgggaat ggataaaacg ggattattgt atgaagccaa
    53941 caaagcccac tctagattcg gaaccaaatt acgaagatca tcctgtgaat ccgtggccaa
    54001 gttggaatcc tgaaaatggt tatttcagag attacgatgt tcgcaagcag acctatcgtt
    54061 ctgtttttgc cggagcatgc ggtgttacat acggcaatca cagtatctgg cagttcagga
    54121 atgaacgcga cccaaatatt aattttgcgg agagatactg gactgaagca ttggacagac
    54181 ccggtgcatt tcaggttgga tatttgaaaa aacttattga atcacgacca tcatcaagca
    54241 ggattccaga tcaatccatt attttggaag gtcagggaga aaaagaagag tatatttgtg
    54301 ctttcagaaa tcgatcaaat gattatgcca tgatttatat tcctgttggt aaaacaatta
    54361 caatcaaaac atcatttatt aaaactaaaa aactgaaaat cagttggttt aatccacaaa
    54421 catgtgagac aattcaaagc atagaaacaa ttaataccgg taaaatgtgt gttacaactc
    54481 caacaaccgg ttttggtaaa gattgggttt tgatagtaga aaaagattta gtaaatacag
    54541 caaagtaaga ttttattttt atttctaagt acatgacaaa aatttaaaaa tatcagaatt
    54601 tgatttaaaa cccgcattta aagcactatt gcaatgaagt tcatatttga aataactttt
    54661 tcccattcta ccattgttca gcattgtgat tggctattca tgttcgtgta aaaaaaattt
    54721 cgaccagata tgcccaacaa aatgcaatgg ttactatagc gacaagtttt ttaatcctat
    54781 aaataaccct caaaagagtg tcttctaaat ttaatccagt tgttttcatt ataaccgatt
    54841 gttagtcagc tataaagaaa aatttcaagg tctttaccaa aaaatgacaa ttattttttt
    54901 tgattgtcaa gtaattatat cgcaatataa ttattgtaat attcggaata ttattttata
    54961 agtcaagcat ttgtgaatcg atagataatt tgaacccagt tctgaatgaa aaaaaacaca
    55021 ttattattac tgcttctaat atattttgag atgcttacag ctcaaaatcc tatttgccct
    55081 cccggtttga atattgccga cccttctgca cgagtctgga atgatggacg gctatatgtg
    55141 tacggttcac gcgacgaaag ttttaattat tattgttctt ttgattactg ggttttgtcc
    55201 actgatgatt taattcactg gcaatataca ccgaatgttt ttgcgtctaa aggacaatac
    55261 gatcaggtgc catttaacga taatttgttg tttgcacctg attgtatgta taaaaatggc
    55321 acttattatt tgtattattg ccagccggat ggcattcaag gagttgctac cagcctttca
    55381 ccaatcggtc cgtttacaaa tgctaaagaa atttatctga aaaataagaa tcaaatagat
    55441 cctgctgttt ttgtggatga cgatggtcaa gcgtattata tgtggggtca atttcaggca
    55501 aaaattgcca aactaaaacc taatatgaca gagattgata ccactaccat tgttgataat
    55561 ctggttactg aaaaagagca ttttttccac gaaggcggct atatggtcaa aagaaacgga
    55621 atctactatt ttgtttatgc ccatgtcggg cggaaaaata tgcctacctg cattggttat
    55681 tccacttcaa aatctccttt gggtccattt aaatacggag gagttattat cgacaacgat
    55741 ggttgtgatc ctgctaactg gaacaatcat ggatcaattg tcgaatttaa aggtcaatgg
    55801 tatgtttttt atcatagaac taccaacgga tgtgtcaatt cacggaaagc ctgtattgaa
    55861 ccgatttatt tcaacacgga tggaagtata accgaagtag aaatgacttc gcagggcgca
    55921 ggaaaaccgc ttgatgcttt ttctaaaatc caagcagagc atgcttgtct gctttcgggt
    55981 catgtccggg tagaatctta ttccttcgac acaaataatc ctttaaatcc gactaacagc
    56041 gatcaactgg ggcaaattga agataaagac cgtgctatgt ataaatacat tgatttcgga
    56101 gaaggagtcg attccgtctc aatacgtatt gcacccggat taaattccgg aaaaattagc
    56161 tttatactgg ataattcatg gtcattacct tttgcagaaa tagctgtgga cgggggagga
    56221 gatggtaaaa catggaaaat ttttagctgt aaagttaagc gtgttaatgg tattcatgct
    56281 ctgaatctca catttagttc aactggaagc aatactttta atatagattg gtttacattt
    56341 aaacatcaat aattcaactt aataaccaat attatattta tttcaaagct taaataaaaa
    56401 aaataaaaaa ggtatttctt ttgtatcatg gtcattttat tttcattttt tgaacttaaa
    56461 taacacagtt cttgtaataa taattaacac tttaaattaa tgaaaaaatt agtattgctt
    56521 gcatttattt tttccacaat ttttgctttc ggtcagttac caacatttaa aattagtaaa
    56581 aaagtcatcg ctgatattga tgataaactt ttcggccaat ttatggaaaa acccagctgg
    56641 gacggcgaaa taggaggcga tggtgcaatt gataaaaaaa caggttctgt ttcaccggat
    56701 gtattggctt atctaaagga aatgaaaatt ccgattatac gctatccggg aggtaccgat
    56761 gttgattatt atcagtggta taatttaata gataatgtac cgggaaaaca ccagaagcgc
    56821 ccggcttacc gaaattattc tgggacaggc aagataactt ccgataatcg ccttgggttg
    56881 gatgcgtttt taactctttg ttcagagctt aaatccgaac ctcttttggt ggtcaacatc
    56941 ggagatgcat ttttcaggaa aatcagtatt gaggaatcgg caaaaaatgc agccggattt
    57001 gtagcatatt gcaatagcaa ggtgggaaca aaagtttcgg aagggatgcc tgattggcca
    57061 gcaatcaggg caaagaacgg tcacacgaag ccttacaatg ttcgttattt cgaaattggg
    57121 aatgagattc aattatttaa gtcgatgaaa gaaaaagatg tgaacgatag tttggtaaat
    57181 cattatgtca attgcttgga ggcaattgtg aatgcgatgc ttgacgttga cccaaatatc
    57241 aagattatta ccgatggaga aataaaagaa gtttctgaac aaataaaacg cagactgggc
    57301 aataaaatca gttatttagc atgtcattat tatttaccat gggaaattaa tacggtaaaa
    57361 ttggatacca ctacaataga tataaaatca ttaacccaag ctgatatttg gaatgcatgg
    57421 gtggcaacgc cttatattca tccggaaaca ggacaatctt attttcccaa aacaggcaaa
    57481 tataatcatg cagccgatgc cggatacgat ttggctataa cagaatggaa ctggaatggt
    57541 tggtggaaca aaaagatctt accagatgca gcattaaatt cgctttttgc aaaaggaata
    57601 ggtgcagctg gttttataca tgcaatgatg cgtgatgccg gacggatgaa aattggttgt
    57661 cagtcgatgt tggttggcaa cgtatgggat attaatgcca ttcgggtgga cactaccgga
    57721 aagatgaagg ctcgattttt cccaacgggt aaagttacca cactttactc aaaatatcac
    57781 ggaaaccaat tattggagat gagtgaaaag aatcagttgt tttacacaca gccatataaa
    57841 atgaactcaa tcgaagctgt accccgggtg acatatgtcg atgcattagc atcgcgcaac
    57901 gaaactacgc tttacatatc gttcatcaat cgaaattttg cgaatgatat ggacgtggaa
    57961 attgatttat ctgattttaa aactgattca aaatataaac agcacatttt tacaacaaac
    58021 gatatgataa caaacgaatt gggttatttt gaagataaga ctgaaaaagt taaaaaccat
    58081 aagccgctga ttgttcatct tccaaaacac tcattatcga tttttgagat taaaattttt
    58141 tagaaataag ttctttgcgt gaaaaaatta aaagagggtg ttccaaaagg acactttctt
    58201 ttttagatta agcagctgat ttaacccaac tttccattca ttagccggaa ctcattgcta
    58261 aacaagtaaa tatactgtct ggaggatgag tttgggtact acaaattttt cgtcgtttga
    58321 atagctgttg tttgtacttt aacgctttgt aaatttcagc aacttcctga cttcgacacg
    58381 attcaagagg tattttgtaa cgatcacaca atgggatatt tgtatttttc gcatcaccga
    58441 aatgggtgac gcaagaggaa aaagtattac atttgcgtat gtttgtacgt aagaaaaaga
    58501 atcgttcagg gactaccagt gtcgttgtag ccgagaaaag aatatgttaa ttgagagaaa
    58561 cagtcaaaac ccagatgtta atactgaaca aactctgatg ctttggccac aaatacttta
    58621 tttacaggtc attattttat tataaatcct ctattcgaca tgttttttat cattctttcc
    58681 actccaaata agttccgtca tgttttttta cttttacttt cttatcctta aaagtaattt
    58741 ttagtacagt agcgtatggt tcaattaaat tggttggcaa gctgataaac ctccagcctt
    58801 tgcggttgtt atcgctgtga taatcatcca tattacgcca agttagatgt ttccccgatt
    58861 ttaaatggac aatgctctca atttcacctt caatattttt caaaaccaat tccggagctg
    58921 tttcgtaagc aataagataa atggttgaac ctcttcgggt agagccacca ttaaacaaac
    58981 ccaatggtaa gccttgttcg gtatcataaa ttgcttcggc atgttccgtt atccattttc
    59041 ctagaagttc catcgatttt atttgaggtt gtgaaatgct accgctataa tcaggcgaaa
    59101 tgtttagtag cagattccct ccctgagtaa taacatctcc caaaatacgg actgtttctg
    59161 atggtttttt tatacttttt tgactctcag gaccatccca attgtatccc aaggtagtac
    59221 acagctccca catgccattg cgtttatcta ttggaattac catctcaggc gttgaaaaat
    59281 caccaatatt tgcacttcgt aatcgattat tcaatacagc ccagggttga tacttgttaa
    59341 tcgtatcgac aatttctttg cttcgccaca tatcagcgtt gcgctcccag tcgccatcga
    59401 accaaaatag atcgatacgg ccataattgg tacataaatg ttttatttca ttcagcatgt
    59461 tgtcggtgaa gcgtttccat gcaacaaaat cactttgttg ccaaatcact tctttaccat
    59521 tgttttgtgt aacgagtgtg ggataatcat aattaaattt atcaggtaaa ctaaagtaca
    59581 accctacttt tattccctcg taacgcatgg catcgcaaaa ttctcttact aaatctcggt
    59641 tcgcaggagt gcttttcaga gccgtgaatt gattgtattc cgaatcgtac agggcaaatc
    59701 ccaagtgatg cttagttgtt agaactacat atttagcgcc ccatgattta aacattttag
    59761 cccatagttt agggtcatag ttattagcag taaatttttt agcttcatca aaacacttaa
    59821 tttgaaactc ttcgtagggc attctttcac gatccttgtg cgacacattt aaagtccaat
    59881 gaacaaaaac accgtatttt gcatccttaa accaacgttt atcgggatga agcgtatggt
    59941 tttgctgaca aatactactt aaaacaaaat tgctaaagag caatgtgata aataaaaatc
    60001 tcattcttca attgttattt tttagagagg tttttcatca taccttatag tatcggtttg
    60061 ttttatctta atattactat ttattagcag ataatctttt acattatgag ccgatggttt
    60121 cgctaaaggt gcataaatat atcccgaatc gacattttta gcccaaacta tactaaattc
    60181 agtggctttt ggggttgcat aatccggtcg atgcacgcga ttgctgccat tttcgatacg
    60241 ttctacgaat ttccattgag taggtattgt atcgtgtggc catgtaatat tcaaattgtg
    60301 aattaccagc ccattaacat tttgtgctac aatagcagct tttgcctttt tagcatcaat
    60361 atcaattgac gaaaactgat tactggttgt tccttcggca tatttgcttg ggtcttctat
    60421 cataggatat accaaattaa catcccgcaa ggtgatgttt tcaattagtg aaccttcaat
    60481 tgccgttaac ataattcgtc cttcggtagt agccgtaaaa ttgctgattg aaatatttct
    60541 gattttaccg ggattacctt tccctttcca aatgccaatt tgaatcggac ggttcaatat
    60601 cagtggcgca ttggtattac atacaatatt ggatatattg atgttttccg tcgttcctcc
    60661 gcaatcgcca taaattccaa ttgcccttga cgattcataa ataatgcagt tgctaaatgt
    60721 aacatctgca atgcgtttaa aattttcgcc caattttaaa gcgacgcaag ttgttttcat
    60781 tatacaattt gtaacggtaa tattactcga ctccctacta cttgggaagg ttttaatgca
    60841 tactgcatca tcgcaagttt cgatatacga gttagctatc attacattgt tacagcccga
    60901 tatatcaatg ccatcgttat tgggtccgta taaattattt accaaacgta ttccttctac
    60961 taaaacatca tcacaatcaa acaaatgaca tgtccactct gggctattgc tcaatgttac
    61021 tcctttaata acgatattct tcgaattgtt gatttcaatc atattgctta cccgcccatt
    61081 taatggtttt atccagcggg gtaatgtatc aaaaggctcc caaaattttg tgccattacc
    61141 atcaatcacc ccttttcctt ctatcgatat attttttaca ccatctgcgt atatcaggta
    61201 tgcacggttt ggatttggat aaacactgat ttttgttttg tgaaaaaggt aatcggcatg
    61261 cttgtctgac cctttcaggc gcgatccctc attgaagtaa agcgtaacat tatccttcag
    61321 aacaatagtt cctgtaagat ataccccttg tggaaaaaca actttacctc cgccatttgc
    61381 atttgcctta tcaattgcct tttgtatggc tttggtcgac aatttggtag agtcgttttt
    61441 agctccaaaa tctgtaacat catagtatcc acttaacgac ttgcatgagg acagaaacaa
    61501 aaatacaaca atgaaccatg aaaatttact cataaaaatt atttataaaa aaacgacttt
    61561 acacattatg ataattattt tttacttttc gcatcgaatc caacgcccac cattggcttc
    61621 gatatttata gatgagattg aaactttttg ttgtgtaacc gcatcgatag catggcggta
    61681 tttataacgg ttgagtttca aagatacatt tttggcactg ccatcattca atataaagta
    61741 atggtcagcc atttcgctcc ccaatctata cacaatggca tttttacatt catacggctt
    61801 ccttatgtct tgcattatta catttattac ccaattttcc attggttcat ttcctttgtt
    61861 tttcatagaa aaagatgcat cgggtgataa aataattgct gaacctttgt cgagtctatg
    61921 ttctgtaaca gccgctgcat tgttgttaaa tttttgtaaa acatcagcag atgtgggttt
    61981 aatatcgagt aaaaaacccg tgaggtttag gtttgccaat tggggcttta cattgttagc
    62041 atattgataa tcggcgatag aagcaccaaa aatacgttca aatgcagaac cttttccggt
    62101 atcgagcact ttgccttgct cgttccacca tgctccgggc gcatcaagta ccaatcggcc
    62161 gccatcgcga acatattgct cgaaaatcgg gaataaatct tcattgattg ccacttgtgc
    62221 cggaagatag attactttgt aacgcatagc caatcccgct tttaagtcgg aaggcgtaac
    62281 atgttcccaa ggtatgttgg cattgataag tgctctactt ataccaatac gagcctgaac
    62341 cggataattt ttgaaatgtg tgcggtttgg tacggcacaa gcagcccaaa tggcttcatt
    62401 gtcccaattt acaaaaacac ccacataagg ttctttgtgt gctgcccaaa gttcatcacg
    62461 gtatttttca gcggcctgtg ctatttgtcc tgcccgaatt gctctttcgc ccggttcgtt
    62521 ctggcggttg agcaaagcat attcgcccga ttcccaacct gctctcctgt aattccacgc
    62581 ccatagaccc actcctttga gtcctccggc taaatatgaa agaagcattt gtgtaatggt
    62641 accaccatcg accgtaaaac cggctgttga ttcagctcct ttgcgatccc aacctttacc
    62701 tccactaaac tgctggggtc cacctgtaga ctcccataca gctgcccaac cccctttaaa
    62761 ccaatcggta gctaatgacg attgcatata aacacatcgt gtaacctcgt aatccacctc
    62821 ttcgaaatgc caggccaaat gaatggatgg gtaaaaagag ccccggtctt tcatcacatc
    62881 ggcaataccc tccatatcga tggcacgcga tgcaaaagga aggaataacc ccatctcacc
    62941 tccagctctt accggttgtt caggatgatt ggagacaaca aagttcttta cattttttag
    63001 atacatatcc gctttaaaac gcaatacatc ctttacaaaa ccatattcac gacctgaaga
    63061 gggaataatt tcatcattac taggagcact aaaaagaaac ttatcatttt caaagtcgtt
    63121 ccaggaacga tatggtattt tttttattcc aacttcgtat tgattccacg ccgttacaag
    63181 ctgatcaatc gattgatatt tgttttttac ccattgttta aaaaggttta tatcagatgg
    63241 tcgaaggtat gcatccggtt catgcctgta gggtatcaga gttgtatcag aaatgggctt
    63301 tttccaatcg gcaagttgct tttgaaaaac attcttttgg tattcaagca ttttcggatt
    63361 tttacggatt tcttcttttg tggcactaag cggaatgttg agtttacgta ataagtctgt
    63421 tgtgataggc tcccagcctc cttctccata ccaaaaagga ataacatctt cttccaaggc
    63481 tattcgcaat atctcttttt cagaccattc aggtgtagcc atggtttgct tcaggttatg
    63541 aaaacccagc cttttcatca tgcggaagtg ttgacgcata tcttgctcgg tacaacctgc
    63601 ccagggtaga aaaaccaccc caaaaggcat tggcgcaatt tcacgtattt tttgctgcat
    63661 aggcgaatca tgcaatttat cgtaaagtgc atgtaaaaat tcatcatttt tttgagctgg
    63721 taacagaata ggtatgagta tcagaataaa tagtaacttc ttcatttgta cgtgtatttg
    63781 atgtcgtttt aattgaattt ataaagatta cagaattttg atattggatt ttttgatatc
    63841 aaataccggt tttgttttcg atgaagattt gagcaaaggt gttcggaaat aaccaccttg
    63901 tacgtttcgt gcccaaatag cgctgaactc ggtttgccgc gctttcgtat agtcgggttt
    63961 atgaatgtgc gatgtgccgt tttctatcct ttcggggtgt tgccaattga ttggagttga
    64021 gtcagtaaga ggccaatcga ctatcatatt ttcaattacc aggtttttaa tattttcggc
    64081 cactaaagca gcattcgcag ttacagcttc gggatgatcg gttggttttg ggaattgtga
    64141 acttactttg gcaccaatat atcgctgagg gctttcaatc ataggatagc gcaaaacgat
    64201 atcgcgtaaa attacattct ctatcaatcc tccctcggca cttgtgagta aaatacgtcc
    64261 ttcggtttcg catataaaat tgctaatcaa tacattgcga actgtacctg ctttgctgtc
    64321 tggatttaat ttattcacta aaatttgaat tgggcgatta aaaaccaatg gtgcattagt
    64381 attggcaaca atgttaataa tagcaatgcg gtcgtaagtg gcaccatctc gcaccccaat
    64441 cattacggca cgcgatgatt tatttactac gcaattatta aacgtaacat cggtaatgtc
    64501 tttactcgat tcggtacatc ctaatttcaa tgctacacac aaggtctcaa tcacacaatt
    64561 tgttaccgta acacggtggc atgaacggct gtcgggagtt gttttcaggc aaatggcgtc
    64621 gtcgcatgtt tttatgtaac aatccgaaat tattatatcg ttgcaaccag taatatctat
    64681 gccatcggaa tttgggctgt acaaattgtt gatgatgttt actccgtgaa ttttcgccac
    64741 atcgcaatcg aacagatgca ggttccaacc accacctgtt ttaattgtta catcgtggat
    64801 ataaatattt tgcgagcgtg taatttcaat tagcgggctc accttgagtt ttttagcttt
    64861 gatccaacgg ggtataacca tattgccatc ggcattgcgt tcatattctt cccaaaaata
    64921 ctcgccattt ccgtcaatag tcccttttcc ctcaattgtg atgttttgtg cattatcggc
    64981 tactatcaga tgatagggct gacgatcgac attatgtccc caggtaagtt cggtataatc
    65041 ctcgatacgt ggtgagccaa gcaaagtagc tccggcagca atatgcagcg ttacattcga
    65101 ctttaaaaat attgttcccg aaacgaatac accactatct aaaagtacag taccgccgcc
    65161 atgactatta gctttatcaa tagcttgctg aattacagcg gtgtttaagg ttttaccatc
    65221 agggatagca cccagttgcg ttggtttata tatattggga taagctgttg gagttgttga
    65281 aaacaaagta actaccgtag caaacaaaac aaactttttt cgcataaaaa tttttttatt
    65341 aacaaatatt ctgtttaaat gaacgtcaga tttcctcctg taagtgcgaa cgaccttctt
    65401 ttcctgattt cggtgtagta tgaccagatt ttgtaaagta aaaggattgc tttttatcac
    65461 ccacctttat aacaaactca ccatcttcca atagcatttc gcctttttcg tttggaaatg
    65521 tcaattgttt ttcaggtgtt atgtcaaaat ttacggtttt tgattcgcct ggctccaaat
    65581 aaattttatc aaaacccact aaattggtgc gtgcaggcga gatgcttcga accacatcgc
    65641 tcaaatacca taaaacagct tcgctgccac cgacttcaga tttatttgta acctgcacgc
    65701 taattttcaa agtctgcccg ggtttaacga tagagtcgga taatataaga tttccgtatt
    65761 catattttga ataagataaa ccatgtccga atggccattt ataagtgtat gtgtcgtgta
    65821 cttttacatt atatggcgtt atatgcccta ccgaacgcgg ataggtaaaa cataatttac
    65881 ccgatggatt ggcatcaccg ttgagaatat ttgctaaagc agtggcacct tcataaccgg
    65941 gtaatcctgc ccaaataata gcatttgctt tttctgccac tgaagtaatc acacgtggtc
    66001 gaccactgaa aatggaaaga atgtgtttgc gaccagtggc ttgtacaatt tttattattt
    66061 caagttggtc aacaggcaat gtcaaatctt caatattgcc acgaccttcg gcatgaggtt
    66121 cctcacctac cgccacaata atgatatcgg atatttgagc tttgttttga aatgcactta
    66181 agttaattaa tggattagaa attgtatcaa caactgcact cggatatttt tttaacaaag
    66241 cggtaaaaac agtttccata tcggcgggaa acatttcttc gggagcacca ccccattcca
    66301 atgtccatgc tccacagata tttcgtttag agtgagctgt tggaccaacc gccaagatat
    66361 ttttaatatc tttcagtggt aacactccat cgttttgtaa caaaaccata ctttcggtgg
    66421 ctgcctgtaa tgcagccaag ttgttttcag gaagtcctat acgcttaaat ctatcattgc
    66481 gtggataagg gttctcgaac aacccaagtt caaacttcaa ttttagaata cgagacacac
    66541 ttttatctat cacactcatt ggcacttctt tggataaaac caaatccttg acaacgtcgc
    66601 agaaagtagt ttgtgtagct atcatactca tatcaatacc tgctagtaaa gccattttgg
    66661 tagcctcgcg ttcatcgcgt gcaaccttat gttggccaat taactgaaga atatcagccc
    66721 agtctgttac aactactcca tcgaatccta attctttacg cagtaaccca tttatcaaac
    66781 gttgcgaggc atgaacaggt acaccgttta cttccgagct atttatcata aaggttttta
    66841 cgcctgcttt aatagcttca cggaatggtg gcacgtgata ttcgtataat tcctgatcac
    66901 ttatcacagc cggtgttctg tcccacccag tcttggattc gccatagcca ataaaatgtt
    66961 ttgctgtagc tgcaatttta tatggcaaag ctgcttgtgc ggtttgcata gctttaatat
    67021 aggaggttcc catttccgaa atcaaatgcg ttgattcgcc aaaagactcg tacatgcgtg
    67081 gccagtaagg gttgcgagcc agatcaagaa caggggcaaa gttccatggc tgccctaaat
    67141 cagctatttc caatgcagta acttcgccca ttttggcagc caaactctta ttaaatgtac
    67201 atcccaaacc taaagcctga ggaaaaattg tagcgccaat tacgtagttg gcaccatgaa
    67261 tatgatcgtt gccataaatc attggaatac ccaaccgtga attttggatg gctatggatt
    67321 gtagctcata agtgaattta taccattgct caggctctac ggcaaagcca accagaaaag
    67381 aacctatatg ataatcttta attaatttct tcgctttttc ggggtctaaa tacggacgga
    67441 aacgacgcga cttggggtca cctggtttgc tgtcgttcaa ttcactcgaa ctgatttgtg
    67501 acatttggcc aattttctct tccagtgtca ttcgcttcag taagtctttt acgcgattat
    67561 caattggttg cgaagggtct ttatacgctg gaaactttgt tttggcagac aaagggctat
    67621 aattaaacaa ctgaaataaa aaaaggcaga taagcaaaaa tgttttattc atgatttaaa
    67681 aatacgtaag ttagttatta aaacaactga aaaataaatt ttaatctttt acagggataa
    67741 ataatacagc atcggctgtt cgctaagtac tttgtttgca tcgacatcct gaacacccac
    67801 ttttgcatct atagcctgac aggcttcaat ggctgccgac tgggacataa ccataaatac
    67861 tggttccata cgaatagaac cgtatgcaat gtgtgaagca gacatacaaa ccggaactat
    67921 tagattgctg gcttcctttc tttttggtac aagcgattga tacgaaatgg ggtaaggtgg
    67981 gaagccaccc acttcaacat taccttcatt ttttaccatt ccattcacta catgacggtc
    68041 gcagttgtgc gaatccatgg tatatgctgc ccatgctata gggtcagaaa ctatttcttt
    68101 tccttggcaa tggtgctgtg tcataacctt ttcgccaacg agccgacggg cttcacgcac
    68161 ataaagctga ggtgaccagt tgctatttcc tatgtattcg tccttaggat aaccccattt
    68221 caacatctcg ttacggattt taagtgggat tcgctcgtca tgtcctacga aatacagtaa
    68281 gccttttgta taatcttcgt gttctttaat aattcttttc cgttcgtcat aacttgcatc
    68341 ggcataattc caattcatgc caatcatgtc ggtagagaac cccccacggt tattaatatc
    68401 tgtttttcga ttgggcatca tcgaccaaat gaaaatatcg ttgagcgaat tccatggttg
    68461 cttttctttt agccggataa gcaattcata tttttcaggc tggtaattat ctggttttgt
    68521 gatttctact ttgtttgatg gcttgtcggt aaggcaaatc cgataattgt atgcttgtac
    68581 ttttttatca ccggtaccat tgggtaaaac cgggttgggc gatatgcccc aaagtaatcc
    68641 actagttgga tttcccttct ctttgtaggg atcaatacca tcgggaaact gatgtgcatc
    68701 aagaagttga accccattat atgtttcatt gtaaagcgtg ttgctttcgc ggcctattgt
    68761 atatgaagca cctgctcttg ccatcaaatc gccttcgtaa ctgcaatcga taaatatttt
    68821 ggcgctgatg gtttgattcg ttgctttgga aggtttaatt gagttttcta taacaatgga
    68881 ttggattgtg ttcttagatt tgttgacctt gtttaatctc atgttataca acacgttcag
    68941 atctgcttct tttacataat cattaaaaat tccatcggct accgatggtt caaatatcca
    69001 ttgttcaagt ttgccgtagt gagttcctaa gcgacgataa aaatcacgcg aaagtccttt
    69061 aaccacatat ttattgccaa tgtcggtata tcccaatccg tccgtcgata atccgcccaa
    69121 gtttgaagat ggctcaatga gcaaaacgct tttgcccgat ttttgcgcag tgtaggctgc
    69181 aattacacca ccggaatttg caccgtaaat gcaaatatca acagaagttg tttttttccc
    69241 aaaaaccgta gaaacaagaa acaatgtaac aataaatgat atcgtatgta attttggcat
    69301 gttttatttt tttagaatag ttagttttga ttttccaatt ccaatgtttt tacctacact
    69361 aacccttttg tttgtttttg aaactgattt caaaaataca ttcgactctt tatttgtaat
    69421 acagttttca atcagtacgt tgtctgtgtt gcgaagttct ataaatgagc cttttgctgt
    69481 tggggcttct tcaccggaga gtttgtcgag cactaataca tttacttttt caaaagtaaa
    69541 agcattgcgc cacaacggct ctaccgatgt ggtatcccat tttaccgaca catctgtaag
    69601 ccacaaatca ttcacattta cagctctgaa agcatcatca gcacgtttat ctatgtagtt
    69661 ttcaggatgc ataaaaaact gcacattgtt cattcggata ttttccaatg gtttatcttc
    69721 aaatccttcg atttttgaag ttcccatacc atgcgctata atgttgttga atgtaatgtt
    69781 acgaatacta ccaacgcggg aatccggtcg gcgtttcagc acaaccaacc agatggggtc
    69841 agcatttccc caccagttga aatggcgacg cgagcattcg accattatat ttgaaaactg
    69901 tatattttca gcagttccac catcgcgaat tacaatgctt aaaccacggt ttgaatttcg
    69961 aataacacaa ttattaaacg aaatataccg gaaatcggca tgactttctg ttcctaattt
    70021 caatgcagtt gaagaagact gaagcacaca attgttcacg gtaacgtttt cgcacgaacg
    70081 ggactctcca tgtaaatttg tcgttttcaa cacaatggca tcatcacctg tttccacaat
    70141 gcaatctgaa atcgtaacgt ttcgacaacc atcgatatca attccatcgg cattaattcc
    70201 tttttccaaa tcagaaaaaa ctttcacacc tgaaatggtc atataatcgg tccagcgtgc
    70261 atgcaaagtc caaaactgag atgcgtttag tgttatatcc cgaatagaaa caaatttgca
    70321 attctcgaaa taaatcatac acgtataggg cttgatatga tagtagcgtt tcattggcac
    70381 tccggctttt tgtgcatttt cagtaatatc tttgataaat ccatccacca tgtccaaatc
    70441 gccccaatag tgtgttgcct taccatcaat cacaccattt ccctcaatgc taatgttttg
    70501 tgcatctttc gcatacaaca aacaagggat aatatcacca ctgttgtttt gtactgcttt
    70561 ataatcattg gtatcgcgac tggcaattat tgtagctccc gtttcgacac gaaaagtgat
    70621 attactttta agcaccaaac taccggtttt gtaagctccc gcaggaaata taattttgcc
    70681 acctccggct ttgtcgcatt tatctattaa ttgctgaatt ttagcagttt gaactaaatt
    70741 tgaatcactg acaacgcctt cgttcaatac attaatttcg atagcattag ctgaaaaaat
    70801 aaatattgaa agaataaaaa gtagtgcaaa atttcgtttc atttgtttgt atggttttaa
    70861 gataatttct ttttgttttt tgaatcagtt ttgtttgttt tgtgaaataa cagtttataa
    70921 cacaaagtat cacaaagggt tatgcaaagt ttcacaaaga aaaaacgtta ttttacttta
    70981 atataatgga attttccgat gaaaaatact cagcaccatt ttcagaattt actgatgttg
    71041 tgttttgtaa ctgaatattt tgggcatttc taaaagcaaa gacatcgtat tttacacgca
    71101 tttcaccact tttaattttt gcttcatttc catcgaaaaa ccatttatta tacaagcgat
    71161 tcggactggt taacaacaaa caatcgtccg gaacaggata tacaggccat ttaactgtgt
    71221 agttttgaat acgaacatta gctgcatttt caacaacaaa tgtggcattt tgcgaacgca
    71281 caaacggtaa attacgggca aaaccaaccg attgtgccac tttcccaatt tctcgtgcat
    71341 cttccaacag attgtaggtg aggttaatat tctcgaaact aatattgtca atcagtcctt
    71401 cggaaatggc acttaccaaa attcgccccg aagtacggaa atgcatattc gaaaaatgaa
    71461 tattttcaat tactccggct ttttcaaccg gttttgcttt cccgaagttg ggatgctcct
    71521 ttatagcaat atcgtatgga ttgtcaatac ggttcgaatg tacttcaata atcctgtcca
    71581 cagcccaacc cgagtcaatt tttccggtta tgcccgaaac aaaaacattg cggatatttc
    71641 cgccttccac tgtacgaaca tcaatggcac gcgaggtatt ttttatcaca caattgctta
    71701 ccgtaatatc cgaaatatct ttacggcttt cgaaaccaat acgaacggcc acacaattcg
    71761 tttcaaacac gcaatttgta actactattc gttcacaact acgtgaatct tcggtggtct
    71821 tcacagctat tgcatcgtcg cctgatttta tgttacaatc gctgataatt acttcatgac
    71881 ttccagtaat gtctattcca tcactattgg gtccatacaa cgaattttcg atggttattc
    71941 cacgcacttt ggcattgtca gtgtcgtaca ggtgtatagt ccaccccggc gattcggtga
    72001 tgagaatatc cttaatttgc acctgttggc atccctgaat ttcaattaac ggggaaggtc
    72061 gatactctat ttcacgaaaa aaagaaaatt cgttttttcg attagggtcc ctgtaagcag
    72121 gtccgtttcc ataaatctca cctttaccac ttatcgttac atttttttga tctttggcaa
    72181 caattaaatg ccagggagta cgatcttcgt gatgtcccca ggaaaggtgc gcataatctt
    72241 cgatacgagg cgaagcaata agttttgcgc cttgcattaa atgcaattca gtattgcttt
    72301 tgagaaaaat agtaccactc aaaaattcac cggccggcac tattactatt ccacctttca
    72361 cagctgctgc atcgattgct ttttgtatgg ctttggtatt gttggttttg ctatcagcct
    72421 ttgcaccgaa ctttgtaatg taaaacagct ctttcgaaaa cgacagatca gcgaccaata
    72481 agcaggctat cagcacataa aaaaactttc tcatttctaa aattttctga ttttaatacc
    72541 attcaatacg gtttcggaac gtattttgtc aaatttcaca accacaccat tatcagcagc
    72601 tttcaccctg aaagtaaaat tggctgctgt attttcacct acactttcca ataaattcaa
    72661 ctcacttaaa accaattgat tattaatact tacattaaaa atccgaccat ctttcaacag
    72721 ctcggttttg gtttcgttac ccagattatt cagaaatgca tctttcagct ctttagcact
    72781 ttgcagctcg gcgaaaagca attccacctc gtaccaacct gccgccacat cgaagcgata
    72841 ttcggtgaga ccaacccgct gcgtttgata aagcggatcg ttaatggtat tataaatcac
    72901 ccgttcgcca ccggtgcgct gtcctttcca gatcttaaac tcttcgccgc ccacataacc
    72961 ccaaccaccg gggcgaaaag gtctgtcggg catccacact tcgttacttt tcgtatccac
    73021 ataatcgaaa tgaccgccca cattgacgca taattcccga aatgaattgt tgttaagcgg
    73081 aaatggaata aagtgataat caaaattgac cacatctgta attgtttttc catttgtcga
    73141 cctgccaatt gctaccaact gattcttacc atttacgaaa ttaagcggag caaaaatccg
    73201 acatatacta tcgggttgaa aattgccaac cgttttgcca ttgagttgaa gtgtaacctg
    73261 ctgacaatta ctgtaaattt taatggtatc ggtaaaagtt cttgcttcca caggcacttc
    73321 aaccggtcgc ttcagccatt ttccggcagc aattttcagc gccggttcgt tttgcaattt
    73381 ggccttatac agataaaaaa catctttggg agtgcggtca taatacaaca aacctttgtt
    73441 attcatatgc gggtcggtat cttttcggcc ttccgaactg aaatcggcaa aattccataa
    73501 agaactgccg gcaattttag gattccgtat aattttatcc aaataaattt cgtgtagttt
    73561 ttcagcccac tgcatcgagt tatcaaagcg gcgtggcaaa cgggcataaa ttcctttatt
    73621 agaacccgca ccgtattcgc tgataattag aggctttgtc ggatattttt gtttgaaatt
    73681 atctacaaaa tcatcaaatc caccaaatcc atttacatac cagccaaaat acaaattgta
    73741 accgcacaca tcggtgatat tattcaagcc ggctttttca tatttctcaa aatcgccatg
    73801 atgagctatg gttgtaagtc gtgtgttatc caatgatttt gctacatcgt tcagtttctg
    73861 tgccagttca cgggttttaa caaataaatt tccccgttca ttttcaggaa aagagtttgc
    73921 ggccaacaac acttcattca taaatcccca aaatacaacc gaaggatgat tgaagtgttg
    73981 taaaatcatt tccttcatat tgtttagtgt attttgttca aaatgctcat ttattccgat
    74041 ttggttcaca accgaaattt cactccaaac taagataccc aactcatcgc aggcatcgta
    74101 aatggcaggg tcgtgcggat aatgtgaggt acgtaaaaaa ttagccccta tctcttttat
    74161 tatttctaca tcacgacgat gaatagcgtc aggcagcgca ttgcttaacc catcaaaatc
    74221 ctgatgacga tttacgccaa tcaattttat tactttgccg ttgagcagaa tatttccctt
    74281 ttcgtctttg gcaatggtac gaatacccga acgcacacag taagtgtcta aaacggtctt
    74341 ggttttagca tccaaaattt cacattcaac ggtgtacaaa tacggattgt cgggtgacca
    74401 aagctgataa tcaggaattg caaacagctg cttaacattt tgctgcatag cgtttttcag
    74461 attaatactt ttactgattt gcgctgaaat aattttacct ttctgatcgg taatacgggt
    74521 ccgcaacatg agctttttcg tttcgccgga actattcact atgttggcgt ctatctgaaa
    74581 acctgcattt gcgccatcta aaaagctttt cacaaatact ccctgaccgg cataattcat
    74641 tttatcgaaa tgtatttttt cgcttacttc taaataaaca tcacggtaaa tgccacccat
    74701 catactgaaa tcgcccgata tgggggcaat ttcagtagaa taactgttgt caaccattac
    74761 gtcgatccga ttcgatttcc cgaaatcaac aaggttagta atatccactc tgaaagctgt
    74821 atatcctcct ttatgttcac ccgctttttg tccgtttacg taaacggcag cctcctgatt
    74881 tgctccttca aaaaataaga atattttttt gtttttcaac gaatcagcta caagtattga
    74941 tttacgatac caagcagcat cgcgataata tccctttacg tcatcccaca catcggccat
    75001 gttccatgtg tgcgggagat ggactttttc ggcattttcg aaagttggaa ttactgtttt
    75061 atttccgggt gtttttacaa attgccaacc ttcattaatg gtttgacatg cataagctgc
    75121 atttacattt aacatcaaca ataaaattat tgatacgata agctttacac tatttttttt
    75181 acaaaaacgt ttaaataaaa aaaagaaata attcattgca ttacatttaa tattgattga
    75241 aatttaatca tttaattcga ctatttaaat aaaactgctt gagaaacata tcaattcata
    75301 aaaagatagg catacatcta tgaattagaa ttcgcaccac atctagactt acgaatataa
    75361 aatattgacg aaaaaaaata aaacgtacct taattaggtt caagaataat caaatgtaat
    75421 ttgagttttt ctattcatca atttaatagt gagaatgtta taatgtttca atttaaaact
    75481 tgtaatatat gttttttcac ttctaaatta tgattatcca caaagattcc tattgtaaat
    75541 tcttggtttg aaatcggagt tgtagcttac agcagctaca agcaatgtgt cgaagatagc
    75601 ttcaccgaaa taacgtcggt taaatttgta aaagaactca ttgaggtagt attgtaaaca
    75661 gtctgttctt aaaaagcgta taaacaaaag attatcaata aaataaatta aaaaagtgta
    75721 tatatttgca agttttatta tatgaatctc ctccgaaaag ttactcaaag agaattggtt
    75781 attttaagac attgacataa aaaggaggaa aaatatttaa aaagtttttt gacaattttc
    75841 atcagaatat aatttatttt ttaaaggtct gatggaaccc atatgtctga atattacatt
    75901 cattcgtgtt tgtgtatgca ataaatgtgg gtttcatata ttattgtatt tcagtaagct
    75961 tttatcagag ttttgatggg atgatgattt taaaagaagg tcagggccgg agcgacgggc
    76021 agtttatttg ggccatcata aaaaatgccg taaaaaacag aaaatggcaa ctatcgttat
    76081 tttaaccttg agcaggtgcg aacttctgtt ttgagacaaa aactacgaaa cattacaaaa
    76141 gaaaaaccta accatagaaa caatgttgaa gcgaccattt ttcagttagg ttaccattat
    76201 tccaatgata agacacgtta tcgccgactt gccaaacact gcttatgggc ttattctcgc
    76261 actttgtgga taaacttcgc tcgaatactg aaatttatag tgcaaacctc tcaaagaacg
    76321 cttaatacat tacaaaaaac tacttttgta acaaaaataa cttttatatt gcaaattatt
    76381 gtaaaatatc gaaacaacaa aaaatttaat gcctctcttc aaaaaaatct tattttttct
    76441 cttttttgaa aaaatcaggt tttaagtggg gattcttttt ttacttcttc aattactgct
    76501 ctttcccgtt tgccagtcaa tgtgcgtcca taatgcttaa tcagctgata ataaatttct
    76561 tcaaatac
//
LOCUS       DAOTAX010000003        55281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_480_length_55281_cov_30.979502, whole genome shotgun sequence.
ACCESSION   DAOTAX010000003 DAOTAX010000000
VERSION     DAOTAX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 55281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55281
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_480_length_55281_cov_30.979502"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1179..2309
                     /locus_tag="PLM04_00665"
     CDS             1179..2309
                     /locus_tag="PLM04_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON01627.1"
                     /translation="MKIIRRILQDKIIDKIAPGKVVLIFGARRVGKTILMHQILESYQ
                     GKTMLLNGEDYDTLTLLENRTIANYRHLFNGIDMVAIDEAQNIPQIGSILKLIVDEIP
                     GISVLASGSSSFDLLNKTGEPLVGRSSQFLLTPFSHREILQMETMLETRQNLEARLIY
                     GSYPEVVMMDNFDRKTDYLRDIVGAYLLKDILAVDGIKNSSKMRELLRLIAFQLGNEV
                     SYDELGKQLGMSKTTVEKYLDLLEKVFVIYRLGAYSRNLRKEVTKAGKWYFYDNGIRN
                     AIIGAFSPLAIRQDVGALWENYLISERRKANLNEELHKEFYFWRTYDKQEIDLIEESS
                     NDLTALEFKWRNKIPKIPKAFQETYPHAKFYVVNKDNYLEFI"
     gene            complement(2557..3705)
                     /gene="cydB"
                     /locus_tag="PLM04_00670"
     CDS             complement(2557..3705)
                     /gene="cydB"
                     /locus_tag="PLM04_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HON01628.1"
                     /translation="MITYSFLQHYWWFLIALLGGLLSFLLFVQGGQSMIFSLSKTEDE
                     KRLLVNVLGRKWEYTFTTLVTFGGAIFASFPLLYSTSFGGAYWVWMIILFCFTLQAVS
                     YEFQTKAGNLLGKNTYRFFLFLNGLLGTFLLGVVIATFFTGSDFVVDKANISNPLSPI
                     ISRWQHPWHGLEALLNLRNVCLGLAVFFLSRTLAALYFINRVDHEMLEKRSRKYLWYN
                     AIPFVVFFLAFVIWTFLSDGFAVHPVSGEVYLSPHQYFLNLVEMPAVMTTFLLGIVGV
                     LYGIIRTLFSENYRKGIWFAGAGTVLAVLGLLLLVGFNNTALYPSATHVQSSLTLSNA
                     SSSEFTLKVMSIVSLIIPFVVIYIFYAWRALEKKRTNVEEINEDPHAY"
     gene            complement(3753..5312)
                     /locus_tag="PLM04_00675"
     CDS             complement(3753..5312)
                     /locus_tag="PLM04_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HON01629.1"
                     /translation="MELLLDASLVNWSRAQFAMTAMYHWLFVPLTLGLGMIMSIMETL
                     YVKTGKEEWKKAAKFWMTIFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGILAFFLEATFISIMFFGWKKVGKNFHLASTWLTTLGATISALWILVANAWMQYPT
                     GMTFNPDTVRNEMIDFWSILFSPVAINKFFHTVISGWVLGALFVVGISCWFLLNKREK
                     DFALRSIRVSALFGLISSLLLLYTGDGSAYNVAQRQPVKLAAMEGLYEGKNGAGLIAI
                     GILNPEKKSYNDTLAPYLFKVEIPKLLSFLGYRDANAFVPGLKDIIEGGYQTASGETL
                     SFEEKRERGKQAIQALADYQQASQKKDTVAAKMYKDRLMKNYAYFGYGYLDHPQQLIP
                     NLSLVFYPFHIMVILGIYFIVLFAGVLILSYRKKLEHLSWLQWICLLTIPLGYVAGEA
                     GWMVAEIGRQPWAIQDILPVQVAVSSISAESVRLTFFLFLILFTSLLVAEIGIMVKQI
                     KKGPEAEKIEE"
     gene            complement(5336..5560)
                     /locus_tag="PLM04_00680"
     CDS             complement(5336..5560)
                     /locus_tag="PLM04_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HON01630.1"
                     /translation="MQRVGFLKNIYSFYSEGFKNMTVGKTLWIIVLIKLFIMFGILKV
                     FFFPDYLNEHFRDDAGKANHVRMELINKSK"
     gene            complement(5792..7519)
                     /locus_tag="PLM04_00685"
     CDS             complement(5792..7519)
                     /locus_tag="PLM04_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON01631.1"
                     /translation="MKLNKKIFLYFFSIFFILFVIVTYFQYHREKEFSRSQLDGQLNS
                     YVHTIHNFIQSEQPTWEELSRFVRLFPDSTLRVTIVDSLGNVIFDSFLPKGTLFQNHL
                     DRPEIAKARKEKIGEAIRYSASTGKNYYYMAQRFPEYYIRCALPYNVNLIAALRANMF
                     FLYFMLFMFVITVFALFIISKNFSRSIDRLRIFTQKAEQDDILDTDIQFPNDELGEIS
                     NNIVQIYRRMLESKADADRQREKLFKHLQISREGLGIFSSDKKEILANTHFIQYTNIL
                     SDQEVKSSDQIFSISEFSEINAFIDDCFSNNETKRKRITIEKNGHSFYVQCIVFQDDT
                     FEISINDISAQEHENELKRHLTQNISHELKTPVSSIMGYMESILENPNISPQRERFFI
                     ERAYQQTQRLNALLQDISTLNKLDEAYKLFAKEPCSLSEVIDEVLNDVHVQIEQKKCR
                     IIKNYPSDMKINGNNSLLYSIFRNLIDNALTYGGDNITVEINCYREDNEFYYFSFSDN
                     GRGVAEEHLNRLFERFYRVDKGRSRKFGGTGLGLAIVKNAVLFHKGNISAKNQPGGGL
                     SFIFSLRKF"
     gene            complement(7591..8280)
                     /locus_tag="PLM04_00690"
     CDS             complement(7591..8280)
                     /locus_tag="PLM04_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON01632.1"
                     /translation="MAPTRILVVDDEEDLCEILQFNLETEGFQVDTAYSAEEALKKDL
                     TVYNLILLDVMMGEISGFKMARMLHDNPKTASIPIIFLTAKDTENDKITGFNIGADDY
                     ISKPFSLREVIARVKAVLRRSESMQMDETTNKILTFEKLEMHLNNKKVSLEGNEIPFT
                     KKEFEILKLLLENKNRVFSREEILAKVWTDDVCVLDRTIDVNITRLRKKIGPYGKNIV
                     TRLGYGYCFEG"
     gene            complement(8599..8946)
                     /locus_tag="PLM04_00695"
     CDS             complement(8599..8946)
                     /locus_tag="PLM04_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON01633.1"
                     /translation="MKKNFFAVVFFALSVFLGGNVMAGENNSSEAKNVSNTKTVIEGK
                     VVDKNTLETLAGVKIMLNGQKVYTDLDGRFTISTFPTGKARLKASLISYEEQDVEIDL
                     STSNSLQIKLAQH"
     gene            complement(9055..9546)
                     /locus_tag="PLM04_00700"
     CDS             complement(9055..9546)
                     /locus_tag="PLM04_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HON01634.1"
                     /translation="MHKILLLMSVIMTLHMSVFSQKSEPSTIVQSDGLFFTDLSQTKL
                     YTGDYREFYDNGSLRLEMYIKDGKPEGDYIIYFPNGKPNEIRSYRNGKFHGTWRTYNE
                     SGVLISEAFYRNDKKHGTWHIWDDNGIMRYEMHYHEGEKIGTWFMWDEKGKLISEKNY
                     STP"
     gene            complement(9746..10723)
                     /locus_tag="PLM04_00705"
     CDS             complement(9746..10723)
                     /locus_tag="PLM04_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HON01635.1"
                     /translation="MVHWGFIGCGDVTEKKSGPAFQKIEGSDVVAVMSRRLDKAKDYA
                     LRHGVKKWYDDPDKLIHDPEVTAVYVATPPSSHAEYAIKAMKAGKPVYVEKPLASSWL
                     EGQKIVQVAEETHVPCFVAYYRRTLPYFLRVKELIDEGKLGKISSVQIRFALPPSQND
                     FRPDTLPWRVKKEIAGGGYFYDLAPHQFDLLDFILGPISEAQGFTSNIAGLYEVEDAV
                     SGIFRFSNGILGSGMWTFSAPENFRCDEILFVGTKATLTCSTFDFSPIVLENGEKVEQ
                     FSEPKPENIQFYLIESIINFLNGKGTEPVSNCYSAIRTNWVMDTILKKI"
     gene            complement(10725..11837)
                     /gene="ald"
                     /locus_tag="PLM04_00710"
     CDS             complement(10725..11837)
                     /gene="ald"
                     /locus_tag="PLM04_00710"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HON01636.1"
                     /translation="MKIGVPKEIKPQEGRVAMVPAGVMELVHRYGHEVFVQKGAGEES
                     GFSNEAYLSAGAVMLDTIEEVYSMADIIVKVKEPIEREYSLIKRNQILFTYFHFASSK
                     KLTEAMMQSGAVCIAYETVMEKDGSLPLLMPMSEVAGRMSVQQGAKYLEKPQNGKGIL
                     LGGVPGVRPANVLIIGGGVVGTQAAYMAAGLGANVTVLDVNLYRLRYLDDVMPANVIT
                     QYSSKYAISELLPTADLMIGAALTVGAKAPHLITREMLKLMQKGTVMVDVSVDQGGCF
                     ETTHPTTHTDPVYEVDGILHYCVANIPGAVPITSTRALTNATLPYVIKLAELGWEKAC
                     KNHEDIKHGLNIVDGKIVFKNVAESLELPYTEYQIS"
     gene            complement(12136..13194)
                     /gene="pdxA"
                     /locus_tag="PLM04_00715"
     CDS             complement(12136..13194)
                     /gene="pdxA"
                     /locus_tag="PLM04_00715"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HON01637.1"
                     /translation="MEQKIILGITHGDFNGIGYEVILKALSDPVILDFFTPIIYGSSK
                     AAGYYRKMLDIQNLNLNIIGSVKELNHKRVNIINCVDENIKVEVGQSTPEAGEAAYIA
                     LERAVEDLKNGEIHALVTAPINKKNIQSEKFHFPGHTEYLAQHFGNTENALMLMVNDV
                     MRIAVVTGHIPLREVPSTLTSELVLKKLQILNHSLKEDFMVVRPRIAVLGLNPHAGDD
                     GLIGDEEKTILLPAMKEAEKAGIICVGPYPTDGFFGSGSFTKFDGILAMYHDQGLTPF
                     KTISMDNGVNFTAGLPIIRTSPAHGTAYDIAGQNVASENSFRQALYLACDVYKNRELN
                     HQLKSNQLKFESKEKENG"
     gene            complement(13218..14336)
                     /locus_tag="PLM04_00720"
     CDS             complement(13218..14336)
                     /locus_tag="PLM04_00720"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HON01638.1"
                     /translation="MKRAFLRFHARIVERIRVSNISTLKFNFILTKTKTTFVLTLLLI
                     MSGCNSNKSSLPGITGSMYEVLVVMDHQQWISAPGRTLVDLLDQDMEALPQPEPVMDI
                     IPCRPEGFSNMFKSSRNIIISDISEKYSQPKVVYARDKWAHPQAIVRITAPNDSSFVE
                     AVQKFGKNILDFFITQERQRQMEYNKSFVNLEARAEIEKIFGIQIDIPSEFKKAVKKK
                     DFYWITNDNPKIRKDIVIYSYPYTDKNTFTKDYLLAKRDSVMKYNVQGEIEGSYMGTE
                     YKYYPPIFREIWVNGGYCAEIRGLWKMFHGAAMGGPFYSHTRLDEINQRVITIEGFVF
                     APGEKKRNAIRQLESVVYSAKLPQEINALKEISVTPKK"
     gene            complement(14423..17344)
                     /locus_tag="PLM04_00725"
     CDS             complement(14423..17344)
                     /locus_tag="PLM04_00725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HON01639.1"
                     /translation="MEKISVDNIYINNKEKKIIIKANEKFGYMPFREENVKHIYRVLK
                     QIFSPSYKNYKIVCMVDGQTIEELIPNFYRTKRKRDETRIFDPKKPKYSLVKNLSKPY
                     NVTNGLERKNIALWASHGLYYEQSISRWEWQRARVMQTVEDLFTLSIVYPYLIPMLEN
                     AGATVLTPRERDMQSNEVIVDNNNKDSLSYREFNLQEVWTESQDFGFANFKSFYLQGE
                     NPFNMGSYRQIHSVEKENKASFVEYIPDFPKAGKYGIYVSYKTLPQSTENAHYTVYHK
                     GGKTKFSVNQTMGGGTWIYLGQFNFDKGRNENGKVVLSNFTGEKEEIVTADAVKFGGG
                     MGNIARSPDQNGILENHKSSEIKNPALKKEKVLSWETEPETSGYPRFAEGARYWLQWA
                     GFPDSIYSPNRGANDYIDDFQSRAKWVNFLTAGSSLHPKGKGLKIPLDLSLALHTDAG
                     TTKNDSVIGTLGIFTVGKGNGKSYNNKVSRWASRDLTDIIQTQIVEDIRMSFNDSWIR
                     RGLWNKSYYEARVPEVPTMLLELLSHQNYADMKLGLDPRFQFLVSRSIYKGILKYLSA
                     LNDEEDYMVQPLPIRQFSCIFVDKNKVKLRWKTRMDSLEPSAVAEHFVVYTRIDDGDF
                     DNGVLVSDTEYLKVLEQGKIYSFKVTSVNRGGESFPSEILSVCSHPHEKGNVLIINGF
                     ERVAPPAGFVIDTTYAGFLNDMDAGVPYLYGYNFTGKQYEFRRDRKWVDDDAPGFGAS
                     HANYETQILAGNSFDYPFLHGKAICAAGYSFVSCSVEAVMNDDINLNDFQTVDLILGK
                     QKQTDVGVRENISLFKTFPLALQQKINLFCHQQGGNLLVTGASIASDMFDRNDALPDD
                     IRFLENTLKIKYRVHQAAITGNVKFVSSPFKQFVRHQFSYYSEPNQYSYFVESPDAIE
                     PAARDAYTFCRYAENNLSAGVIYSGKYKICALGFPFETIVSDAEREAFMKSVLDFFAK
                     P"
     gene            complement(17525..18550)
                     /gene="rlmN"
                     /locus_tag="PLM04_00730"
     CDS             complement(17525..18550)
                     /gene="rlmN"
                     /locus_tag="PLM04_00730"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HON01640.1"
                     /translation="MPIPLLGASLEELTDIVSQLKMPTYTAKQISDWLYKKRVADINE
                     MSNISVKNRDLLKEKYKVGRLAPVKVTVSKDETKKYLFRTEKDHFIETVFIPEEHRNT
                     LCVSSQIGCKMNCVFCMTGKQGFMGQLSASEILNQILSVPEAEKITNLVFMGMGEPFD
                     NTVELLRTLNILTANYGYAWSPYRITVSTIGLIPGMQTFLEKSNCNLAISLHSPFHSE
                     RLELMPIEKAYPISQVVEVLKNYNFGRQRKLSFEYIMFDKFNDTMRHASELVRLLKGM
                     NARVNLIRFHAIPNVSLRSSSDERMIWFCDYLNNNGILATIRKSRGEDISAACGLLST
                     LENKKMS"
     gene            complement(18770..20884)
                     /locus_tag="PLM04_00735"
     CDS             complement(18770..20884)
                     /locus_tag="PLM04_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HON01641.1"
                     /translation="MATLEKIRSKGVLLIVVIGLALLAFIVGDFLNSSSTFFNQSKEK
                     IAVIGDENISIHEFSAAIDQMTEVYKIETGQTNLNEEVVSQLRASVWENMLMEKILTH
                     EAKKIGLSVSPEELSERLIGKNIHPIILQRGAFMDETGQFSRSLLVQFLNSLDVTPTN
                     NEMKQQIDQAKNYWLFWEKNVKNAILQEKYNKLITSAISANSIDAKMNFEARKITVDA
                     AYVMQPYYSVPDSIFKVSSKELRDLYNKRKELFKQEKSASINYVALEIKPSQEDFQEA
                     EEWINKLEDEFRTTDDVVGLVNTNSDIMYDGRYYSEATVPAMLKDFAFRGKKNDVFGP
                     VFENDTYTMARIMETDILRPDSVKLRHIFLTKENEGKADSIVKALRAGADFATLARKY
                     SAVPQTAANGGEIGWLPDGAQGVDKQILENAFKKPLNEVFVIKDIQGTQIMQVTDRTP
                     LRRKVKLAILQRKVTPSSKTYSQIYNQAKQFAAELDAEKFVSRAQEKGYIVQKTENLN
                     ENTEQINNLPQSRQIVRWAFKGGKGDVSDVFDCGNYYVVAMITEKNEKGYMPIEKVSA
                     QLKSEIIRDKKAAQMINNISEQLKKTPDLEALAASLNAEIKMAENVNFTSYQFGLAGF
                     EPYIIGKATIVPLEKVSGPFQGNAGVYVMKTSNKKENTETFNAQQEIIQLNARMLYSL
                     PYLILENLRERTKIEDYRSNFY"
     gene            complement(21008..22261)
                     /locus_tag="PLM04_00740"
     CDS             complement(21008..22261)
                     /locus_tag="PLM04_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HON01642.1"
                     /translation="MNNFTAVLITLFFSAFFSGMEIAFVSSNKLRFELDKKQRSITSS
                     ILTIFYKHPQQYISTMLVGNNISLVIYGLLMAKILSPLLQILGSQFLITFTQTIVATA
                     LVLVTGEFLPKSIFRVNPNFWLSIFSPLLLFFYVILYPITLLATSLSFGIMRLMGIKI
                     NPLANENAFSRVDLNYLVHESFENTEHKQDVDNEVKIFQNALDFSKVKLRDCLVPRTE
                     IVALDYNVDLETLKQTFIETGFSKIPIYKENIDNIIGYIHSSEMFQHQNDWREHINQI
                     PIVPENMAAQKLMKIFLKEKKSIAVVVDEFGGTAGIVTLEDIMEEIFGEIEDEHDTHD
                     FVAEKVGENEYVFSGRLEVKEINEKFHLNLRESDAYDTIAGFILYHHQHFPKLNESIQ
                     IDNYTFKCIKITNNRIELVKMKLQK"
     gene            complement(22525..23070)
                     /locus_tag="PLM04_00745"
     CDS             complement(22525..23070)
                     /locus_tag="PLM04_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HON01643.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSGLMQGQEVSWMPSYGPEQR
                     GGTANVTVILSDEKISSPVLSSYDTAIILNQPSLERFESKVKPGGTLIFDGNGFHKFP
                     TRTDINVYRIDATEYATAHNAAKTLNMIILGGFLKVRPIVKIENVLEGLKKSLPERHH
                     HLIPQNEKALQIGMELIKKVN"
     gene            complement(23207..23968)
                     /locus_tag="PLM04_00750"
     CDS             complement(23207..23968)
                     /locus_tag="PLM04_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON01644.1"
                     /translation="MSNNEIISPENLVYAKPKSLKETYMHYCPGCSHGVVHRIIAEII
                     DEMDIQDKTIGIAPVGCAVFAYNYIDIDWQEAAHGRAPALATAIKRLLPDRYVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLPDMVTSTTPYGRKT
                     ELNGYPLNITNLLAQLPGTCYVTRQSVQSVAGVRKAKKAIQKAFENSMLNKGTSIVEV
                     VSTCNAGWKMTPENANKWMEENMFPAYPLGDLKDI"
     gene            complement(24043..25149)
                     /locus_tag="PLM04_00755"
     CDS             complement(24043..25149)
                     /locus_tag="PLM04_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HON01645.1"
                     /translation="MNKNSKTDNKEEVLLMKGNEAIAHAAIRCGCDGYFGYPITPQSE
                     VMETLMELQPWTTTGMVVLQAESEVASINMVYGAAGCGKRAMTSSSSPGVSLMQEGIS
                     YMVGAELPCVIVNVMRGGPGLGTIQPSQGDYFQAVKGGGHGDYKLLTLAPASVQEMAD
                     FMPLSFDLAFKYRHPVMILADGIIGQMMEKVVLPPFQPRRTDDEIREQCPWATLGKPS
                     NRSQNVISSLALEAEAMEAINLRIQKRYREMEKNEVRYETYMADDADYLIVAFGTCAR
                     VSQKAVEMARENGIKAGLLRPITLFPFPKDIINQLASSVKGMISIEMNAGQMVEDVRL
                     AVNGKVPVEFFGRLGGIVPSPDEVYDALVSKLIK"
     gene            complement(25197..25424)
                     /locus_tag="PLM04_00760"
     CDS             complement(25197..25424)
                     /locus_tag="PLM04_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON01646.1"
                     /translation="MAKIKGTVVVDTERCKGCNLCVVACPPKALSLSKEANSRGYNYS
                     FLSNPDACVGCVACAYVCPDACITVYKMRVQ"
     gene            complement(25558..26325)
                     /locus_tag="PLM04_00765"
     CDS             complement(25558..26325)
                     /locus_tag="PLM04_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HON01647.1"
                     /translation="MKKFFSLLSLFLFVSILSQAQNELFLKKLFIYKGDTLPYRILYP
                     ENYNKSASYPLVLFLHGAGERGNDNEKQLVHGASLFTDSQNRQKYPAIVVFPQCPENG
                     FWAPIKERDNGFSFINSKEPTPSMQMVIRLLKQIRKEEAVDSRRIYVMGLSMGGMGTF
                     DLICRQPKTFAAAIPICGGVSLERLKKVKHMPIRIYHGGSDDVVSPEHSRNAYIELKA
                     LGSTKVEYIEFPGVKHNSWDFAFAQPDFLSWLFSQKK"
     gene            complement(26457..26930)
                     /locus_tag="PLM04_00770"
     CDS             complement(26457..26930)
                     /locus_tag="PLM04_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HON01648.1"
                     /translation="MKIETERKFLVTGAFKSQASKSYRIIQGYLCSDPERTVRVRIMD
                     ISAFLTIKGKSNLSGLSRFEWEKEIPVDEAQELMKICEPGIIEKKRYEVVYRGQRFEV
                     DEFFGENEGLILAEIELENESQEVLKPDWLGEEVTGKPEYYNAMLKKNPYRSWKK"
     gene            complement(27108..30002)
                     /locus_tag="PLM04_00775"
     CDS             complement(27108..30002)
                     /locus_tag="PLM04_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HON01649.1"
                     /translation="MESFLYRIAQTYYNHYEDKISDFTFVFPNRRAGMFFQRYISQIA
                     KKPLFSPEILTINDCFTSVAQFHLADRLSLLFRLYRIYKEKSGTNENFDSFAYWGEML
                     LNDFDDIDKYRIDARQLFTNVTELKQIDRMFNYLSENQLDAIRQFWKNFIPIPKEKTQ
                     EDFIQIWKILYPVYQDFTEELKRENMATEGMIYRNLAERLESEEKIQEWEGKQFVFIG
                     FNALNRCEKTLFEVLQKKEKADFYWDYEAPALRDADNPASTFFMENTKTFPSKFSIET
                     PVEERKKQYFELIAVPSEIGQIKQIYEILNRIYPQDASVQQESWIKTAVVLPNENLLL
                     PALYSLPEHIEKVNVTMGFPLKSTPVSGLIEHVFDLQKRIRNSDEEVLFYHQPVTEIL
                     QHSYITLLCGDDSKNILQQMVENNSIYIRPEQLQINELFTAIFRPQNDAREMLTYLLN
                     ILRILRKNFQQIGFGNHQYRMETDFVIQYYRFLNRMSDILQYQSEGIEMKMDTLMRLI
                     FQYSAFITLPFVGEPLEGLQIMGVLETRGLDFDNILLTSFNEGVFPKNAPTHSFIPYN
                     LRRGFGLPAKEHQDAISAYNFYRLIHHAQKVYFLYNSSTEGFQTGEVSRFLYQLEYLY
                     DTKVERKNITYDIFLKDAVSASVEKDSLIREKLQRFVSSGDDKLYLSASSINAYIDCP
                     LKFYYAQIARIKETEEITETVENDLFGTLFHSSAELLYKPLKDVQLGKEDFEKILRQP
                     SLVDEAISLAFSKHFFKNEQNGKIPPLEGNNLLISKILKKFLMRLLYVDQQLTPFRYL
                     GAEEFCSIRFPLETLNLRVNIKGYIDRIDETENFLRIIDYKTGTGNTTFKLLDDLFDG
                     SLPMKNRPKHIFQVFMYCLLYRSKAEKKIISPAIYYLKEIFSSGFSSSIQFKPEKHTI
                     QVEDFGIFEHEFKEKLTLLLNEIFNPEIPFKQTQIKENCDNCSFMEICNR"
     gene            complement(30094..31563)
                     /locus_tag="PLM04_00780"
     CDS             complement(30094..31563)
                     /locus_tag="PLM04_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HON01650.1"
                     /translation="MNREINFKLVIRMLGSLLLSEGTALLLVFFISLIYRENDGVYFI
                     FTSLLCYVVGFAGFFFGKNAPMSVGKREGSLVVTSAWIIFSLFGSLPFWLSKSIPLFT
                     DAFFETISGFTTTGASILNNIEELSHSMLFWRSLTHWMGGLGIIVISLALLPMFGTSG
                     AQLFAAESSGPTKDKIHPKISETAKRLFVIYFLLTFSETILLRLGGMTWFDAVCHSFA
                     TIATGGFSTKQNSIQYWDSPYIQYTIIIFMILSGVNFSLYYFGFKNKLEKIRQNEELR
                     YYLIVLFGFAIIVALSMIDFSRPISFHKVEESWRNALFTVGSLMSTTGFTTVDYMQWK
                     PFVWVILLIVMLTGASAGSTSGAIKMVRIVIVFKFCYYEFKRLIHPKAVVPVRYSGKI
                     VFQDVVTRVLVFVLLYLIAAILGILILCMSGVNFIESVGGLVACLGGVGPGLGMVGPV
                     GNYSYFPVFAKWFLSFMMLLGRLELFTVLLLFTPAFWKK"
     gene            complement(31586..32923)
                     /gene="trkA"
                     /locus_tag="PLM04_00785"
     CDS             complement(31586..32923)
                     /gene="trkA"
                     /locus_tag="PLM04_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HON01651.1"
                     /translation="MKIIIVGAGEVGTHLAKLLARESMEITLSDENPDRLMDLESNYD
                     LLTSVGSSTSINHLREIGVENANLFIAVTPYESVNMTACMLATNLGAEKTLARIDNYE
                     YLFPENKDFFKKLGVDYLIYPEVLAAKEISESLKTSWLRQNMSFCNDALILLGIKVRS
                     NSEIINKKFSTGYFDHDKYRVVAIKRGMQTIIPKGSDEILDNDIVYFITTPQNLNFVR
                     EQAGKEDFPIRNVMFLGGSRIAQKTLKRLPDHIHTKVLEINNERSMELAEKFPDTLII
                     NCDGRNIDVLREEGIQEMDAFVAATGNSEMNLLACMVAKRFGVKKTIAEVENIDYIPL
                     AESMDIGTVINKKMIAASYIYQITLNASVLNVRNLTSADAEVVEFIAKAGSKITRSKI
                     KNLKLPENVNIGGIVRNGKGMIVSGETSIEPEDHVIVFCISSAIRKLEPFFNT"
     gene            complement(33046..34938)
                     /gene="dxs"
                     /locus_tag="PLM04_00790"
                     /pseudo
     CDS             complement(33046..34938)
                     /gene="dxs"
                     /locus_tag="PLM04_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            35471..37300
                     /gene="ilvD"
                     /locus_tag="PLM04_00795"
     CDS             35471..37300
                     /gene="ilvD"
                     /locus_tag="PLM04_00795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HON01652.1"
                     /translation="MISRLRSTTSTSGRRMAGARALWRAAGMKNEQMGKPVIAIVNSF
                     TQFVPGHVHLHEIGQKIKAEIEKLGCFAAEFDTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMMNAHKADAMICISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGELDGVQLD
                     LIDAMVQSADDSIPDEQIQRIENAACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTI
                     VATHVNREKLFVQAAKLIVENAYKYYRDGDASVLPRSIATREAFLNAMKLDIAMGGST
                     NTVLHLLAVAHEGEVNFTMDDIDELSRHTPCLCKVAPNTQKYHIQDVNRAGGILGILA
                     ELSRGGLIDTNVRRVDGMTLKEAIHKYDITSPHISAEVIQLYKSAPANRFNLVMGSQN
                     SYYKELDTDRTNGCIRDLDHAYTKDGGLAILKGNLAQDGCVIKTAGVDESIWKFSGPA
                     KVFSSQDAACEGILSGKVVAGDVVVINFEGPKGGPGMQEMLYPTSYIKSRHLGKECAL
                     ITDGRFSGGTSGLSIGHISPEAAAGGNIALLRDGDMIEIDIPARSIQVKLSEEELEKR
                     RQEELSRGKEAFTPKDRQRSVSKALKAYASMVSSADKGAVRLI"
     gene            37385..39070
                     /gene="ilvB"
                     /locus_tag="PLM04_00800"
     CDS             37385..39070
                     /gene="ilvB"
                     /locus_tag="PLM04_00800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HON01653.1"
                     /translation="MKMTGAKALIESLLQEGVETIFGYPGGAILPVFDALYDYERKVN
                     HILTRHEQGAAHAAEGYARVSGKVGVCLITSGPAATNAITGISDAMLDSVPLLVITGQ
                     VGASLLGTDAFQEIDVIGITQPITKWSYQIRRAEDVAWAVARAFYIASTGRPGPVVLD
                     FTKNAQSEEVDFQYKPCKFIRSYVPVPEVNFSQIEKAAELINAAEKPFAVVGHGVILG
                     QAEKDLMEFLEKADIPAAWTLLGASAMPSDFRLNKGFVGMHGNVAPNMKTNECDVLIA
                     IGMRFDDRVTGDLKTYASQAKIIHIDIDAAELNKNLKVEVPVLGSVKDVLPALTKLVK
                     PAKHTEWIDSFDIFEKTEYEKVIEREVFPKSGKITMGEVIHKVSEATHHNAICVTDVG
                     QNQMASARYFKFSQSRSLITSGGLGTMGFGLPAAIGAKIAAPDRTVCLFAGDGGFQMT
                     IQELGTIMENNIGVKMIVLDNKFLGMVRQWQELFYEERYSFTKMKNPDFVAIGKAYNI
                     DGKLVNEREELDEAIADMLKDDRPYLLVVNVETKGMVYPMIPAGTSVTNIILE"
     gene            39134..39706
                     /gene="ilvN"
                     /locus_tag="PLM04_00805"
     CDS             39134..39706
                     /gene="ilvN"
                     /locus_tag="PLM04_00805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HON01654.1"
                     /translation="MMEKTLYTVIIFSENTVGVLNQITVIFTRRGLNIETLSVSPSAI
                     AGIHKFTITVITTQDVIEKVVQQIDKRVDILKAYYNTDEDLVHQEIALYKVATDKLLE
                     LGSIEKMIRDYQIHVLELTENCVVFEKTGHYQETQKLFEELSSTIGVLQFIRSGRIAI
                     TKSKVERLSDMLEKMSKLEQKVNATTETQE"
     gene            39748..40479
                     /locus_tag="PLM04_00810"
     CDS             39748..40479
                     /locus_tag="PLM04_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HON01655.1"
                     /translation="MEKEFSYIFKIAPSNVDFQGKLTLASLIEFLMEAAGRHADGNGF
                     GMRQLNEFNASWVVLRIALEINYYPEQYDEIKISTWIEKIQPIKTIRNFCIENAQGEI
                     IASATSEWIMMDLTQRKPKDLLSLEGFEEFATGKEIPAGKLEKLPPVKGELVDKITVK
                     YGDIDINGHVNSIRYVQWLCNCFNLSDYREKQIERFEIHYISELHENENIKIFREEKK
                     KNDFYFEIDSDKKTTCKARISFSEK"
     gene            40552..41598
                     /gene="ilvC"
                     /locus_tag="PLM04_00815"
     CDS             40552..41598
                     /gene="ilvC"
                     /locus_tag="PLM04_00815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HON01656.1"
                     /translation="MAVINFGGVDENVITREEFPLSKAREVLKDEVIAVIGYGVQGPG
                     QSLNLRDNGFNVIVGQRKGGKTWEKAVADGWVPGETLFDIEEAAERGTIIQYLLSDAA
                     QIEVWPRIKPYLTPGKALYFSHGFGITFKDRTGIIPPPDIDVIMVAPKGSGTSLRRLF
                     LEGRGLNSSYAIFQDATGKAFDRVIALGIGVGSGYLFETTFEREVYSDLTGERGTLMG
                     AIQGLLSAQYEVLRAHGHSPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRG
                     ALDWMGPFHDATKPVFEKLYNEVASGREAQRSIDSNSRPDYRERLEEELKALRESEMW
                     RTGAVVRKLRPENN"
     gene            complement(42370..45753)
                     /locus_tag="PLM04_00820"
     CDS             complement(42370..45753)
                     /locus_tag="PLM04_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HON01657.1"
                     /translation="MSKIELRRKQNIFNRLLLIVIIICLNLASAKAWAQKHDEKISLN
                     FENADFKQIMREIELKWDVVFVYNTSDLNEFGIKSLQTNNLDLYETLNTLFAGTDITY
                     TIKDVYVFLRAKKDTNNLTLKESKSIMGVIVDELQQQLPGVYITDKQGNILGISNEDG
                     SFNIYSIPKDSSLYFFMHGFKRETLKLLNNNNFYKVTMRSNNLLLSELLVIGYGSMQK
                     RETTGAIQSLPKFAIQNNVGGNIGNSFEGNVAGLWASNQNYRIRGVSSINSSADPLVV
                     IDGIPQTIGLNDINPNDIASVEFLKDAASAAIFGSRASNGVIVITTKSGDLNSKQKTI
                     AEYRTSINYVTNKPKFIKGKELISLLDAAYYNRYPENKTLEDNNPIKYFPFSPDYANF
                     LGFNRDWLNTYLLNNPLGTDWESALTQAAISQDVRLSFQGGQKSNSYYISLSHKSDNE
                     LVDNKNKKRSTLVLKNDFKISERINTGFNINFTLNTSKNSTFDKLESLFSRSSLLPIY
                     APDGSGKYFDARNINDKKGSNPLYRMSETWDDNINFNGLLLGYFDIKLMQKFAFRSEL
                     SMTPGTSRYRYFQSKDFYREDEAIDPSKSGIILYSRTLSYGFNVNNLLNYNTKINNRH
                     VIKVMLGNNIQSFNSDFNVARFEGFPTNYFQLTNANTEKVLTRQSAGMDGYRFVSFFN
                     RTQYALDEKYFFELNARADGTSRFINDNRWGFFPGLGMSWMLSEENFFSQIKSIDYLK
                     FRTSYGFVGNAEVGNFPAESRVLNWAEYAGSPGFVLDRIANLDVRWEKQLQLNVGFNI
                     SALKNKISASLDWFLKRNSDLLLNYNIGKFQGYFNTEVPINSGNLNNNGFDLDLKTTN
                     YDGLFKWKTEFNISMFNTKISKLSTQQNYIENGINRAYVGYPLGLYYLPLWAGVNPET
                     GHEMIYEVIGPETNKTKTGKLLDAEMLTSVEYRNQSVLITDKTPYPKLYGGVSNFFAY
                     KKFELSIHFSFQLGNWIYHNGMRQNSYVNTYDINNKMVSLQNYWTPENKNSNTPLLYN
                     SQMANRNSTRYLMNASYLRLRNLSMNYTFDSKNTKKFGLGKIIAQINCQNLITLSKFS
                     NGSPELSLQNAGAEANISLGNIGLNYGVATLSAGIKLEF"
     gene            complement(45820..47427)
                     /locus_tag="PLM04_00825"
     CDS             complement(45820..47427)
                     /locus_tag="PLM04_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HON01658.1"
                     /translation="MKKSQFNILQTFALLIISIIMSSCEDFLSSIRNENSQSIESLLY
                     NINDIDVCINGAYGAFCSNSYYNNVVLTQTLGSDEITNTKNPSILPDFLSISVYNYYF
                     SYQCNTDNYQAGSVLMWGSFVTNNSNVVIKSIENKLPNLRNFNDTLNAERLLGEAYLL
                     RACVEYYNNLYVGRQYHPSTLQLPASLYRKRAILGFEDIPEPRKTVEQVHQYIIEDLQ
                     NARKLLPQYYEKTIHPVAYQYRCKRDVAIAMLAKAYFQQNQFDSALVYINQLLGNQTG
                     ASNKYPLQTTNNYNQLFRIVDKTNYQANSKSEIIMGFHGNSAFRPTSTQEWSMFHWSA
                     FQNLQNSDLTNLRFRIVMDSALIKSFLKGDTLNDVRFKNMIYITKNIGKESPAGQWTS
                     LKHAYPTSNVVWLRAAEFHLMRAEIYLHKDNLSSSINELNIVRKRAGLDNYTFAGKTQ
                     LFQAIIDERFREMHFEGIRRSDNIRLASLSDSNAASYLPMPYKLGNIPLGNRALLITD
                     SILHWNDERLYCLIPENEYIYNPALKR"
     gene            47914..50877
                     /locus_tag="PLM04_00830"
     CDS             47914..50877
                     /locus_tag="PLM04_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HON01659.1"
                     /translation="MKRKELIKLLLCVILLTNFFVTTKGQKLDSKTTPIQTVSLVAEK
                     LIKTTDFSYRLELAKKRNYFDDVICVDFGRTFGLDKKATAIAVTTLETQNESILPIQF
                     DHSDGCKITLNNKIIYEHTGKRKLNVKSDERDLELPLKLDLQLAKGINYLQIELSTNG
                     SEWRFYMQPPTDKGAILKTQIQYPKIGLLPLSKIDAKVAEISNWLFMGPFEMNDKNIP
                     VINGNIVFGKMYNGLNNQPITWTIPKVEVLGNVIDPAPWGTTYQWNYHNGGVAWAMQV
                     LSEKTGNPKFNEWATNFCDFHIEGLPFVDYQVNTLNEFKSANHFILNTPLLDFTLAPS
                     LPFIHRLQSEKNFENRAAYEKFIARMLKYAKEEQIRLPGTNVYTRTTPEVYTTWVDDM
                     FMGIPFLIQAAQYVTDPKEKAMFYDDAANQTLEFKKYVWDNDAKLYMHANYSQRPNVK
                     LPYWSRANGWGIWATTEVLKGLPKNHKHYKAILKHFQTHVASLVKLQAPNGFWRNVLN
                     RPDSKDEVSGTAIFTLAIARGITEGWLDRNKYLPIVQNSWNAIQSQIDADGTVHNICM
                     GTMCSEDVDYYMNRPFFVDDTHGTFAVIFAGIEMQNLLDKIEEEKVYKTNMAQIIDQS
                     LKFATQQSIGLAEKLMSEPKRLPRTIDNEGKLLTSSEKWWTSGFFPGTLWYLYEYSKD
                     PKVKAFAENYSERVKNEQYTTDNHDVGFMINCSYGNAYRLDPKPEYKLIIINAAKSLS
                     TRFNHKIGLIRSWDWGKWQFPVIIDNMMNLELLFNATRLTGDSTFYNIAVTHATVNMK
                     HHFRADASCYHVVSYDTITGLPEHKGTWQGYSHNSSWARGQAWALYGYTLTYRETKDP
                     KFLEMATRIADFMLHHPRMPKDLIPYWDYDAPGIPNESRDVSTASIMSSALIELSDYV
                     KGEKSRTYLDAARKQIVALSSPKYLAQLGENGNFILKHSVGSMPHKAEVDVPLTYADY
                     YYIEALLRYRNKLKK"
     gene            51027..53204
                     /locus_tag="PLM04_00835"
     CDS             51027..53204
                     /locus_tag="PLM04_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01660.1"
                     /translation="MSEHNLKIKYCINNFNYDLYNKLINTYEFDIDFSKKKQSLETKN
                     AIWEVDSKFSETSAGTFDAIIQFILLKGTENNASVAVSFEIDNWSKSNYVLLPAAAYN
                     GNRFESRKIPYSPKLNDYRDIGPLKPIIISDVPRLNLHEGPSQIQERSGSMTTPMIGF
                     HSYNQKKGLLLTTTQANIWGDYGISITETRPETGAKAIISISSPLVREGFKYEIANHA
                     IASPDKPANFKIGDTIKFIIRLRLFESEKLSNLYENYYKARYDLTPVSVPNYIYPFSE
                     CFNTIEQKFNTQNFVPEWGYYAVGMRENFLQDWQIGWTGGMISTYPLLFSNNTKTIQN
                     VIANFNWLFPNGISPSGFFWDSGEKGNKWYGGDIRRPQFENLHLIRKSGDGLYYIIKQ
                     FDLMKKRNIKIEPEWHNGVKTVANAFVKLWQKWEQYGNFVDSRTGDVVVGGSTSGAIV
                     PAALALASEYYQNPLYMKVAKLSAQKMYNDYVAKGITCGGPGDALQNPDSESAYAMLE
                     SFSILYEKTGEPQWLEYAKEMALQFATWVMAYDYNYDYDSTCLFGRMKMKTKGTVFAN
                     TQNKHGSPGICTYSGLALMRLYRATSQSHFLELLQEITLSIPQYMSHNTRPIADMPPG
                     WINERVSTTDWFEGIGEIKAGSTWAETAMLLTATELPSVYVDIKFGKIYCFDHLQTQI
                     IKNNQNFIHIKVHNPTNFTSQFNVFLENKEMKSKAWTENQLFD"
     gene            complement(53836..54291)
                     /locus_tag="PLM04_00840"
     CDS             complement(53836..54291)
                     /locus_tag="PLM04_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01661.1"
                     /translation="MKRLFKKLRFSLWIGLLLTGCVVSNPKFFSKIEPTTDLSYGYTA
                     KNPVTIKNADLNNSISSSYYYLSRLRTEKGNKLQLIQRFSVENPNYEKPKIALQNRYT
                     GEPLSYGTGPILDLYILKPENETDTIKIFINPYLKGTVKIPYGLKFEKE"
     gene            complement(54458..55177)
                     /locus_tag="PLM04_00845"
     CDS             complement(54458..55177)
                     /locus_tag="PLM04_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01662.1"
                     /translation="MKKSTLLFLLITFSLQIYAQENHFPQLKKVVVDADMDVYVKYDA
                     SKSTLIQKKRSALPVNNPFRSNDEYDADDILVLETCLEADCFEKYLVFYTPGMSADPA
                     FYIFNKSNPDSAIIGIGALHLYITSNGFFYIDGHTNNTFNTRQKFRIQKDEVKEVRQP
                     FYYVGLKSKTKIPLTLYSDENMTKEVAKLSAGSSIEVLLADPAEYFRYLVKTSFGLVG
                     WVEFEMVYLDDQIEGLFFKGD"
BASE COUNT        17402 a        10628 c         9977 g        17274 t
ORIGIN      
        1 aaaataagag aagttggcgt taaaaaatct tttctgtttg agctccgcaa ggagcgagtt
       61 taaaagattt tagccaacaa acgcccattc tctcagtaga gccgttgcat gcaacggctc
      121 agataaaagt agaatttttg ggcaatgccc atatttttac ctttttgttc ggcgtagcct
      181 cccggccacg tcgttctttt tgctggtgaa ggcatttgcc tgtgccaaaa tctctgattt
      241 ttaataagct aataaggttt tggatagtgg aaggagtgtt catgttatta aggtttgagc
      301 aaaataaggt tgaaggaaat acccacaggg caatcttatt ttttggtctt gacattagcc
      361 ggtgagccga aaaataagag aagttggcgt taaaaaatct tttctgtttg agctccgcaa
      421 ggaacgaatt taaaagattt tagccaacaa acgtccattc tttcagtaga gccgttgcat
      481 gcaacggctc atataaaagt ggaatttttg ggcaaagccc catattttta cctttttgtt
      541 cggcgtagcc tccaggctac gtcataagta aaaacaaaat ttccagattt ttaatatgct
      601 aataaggttt tggatagtgg aaggagtgtt cttgttatta aggtttgagc aaaataaggt
      661 tgaaggaatg atgtcatttc atcagaatat aatttatttt tttaaggtct gatggaaccc
      721 acatgtctga atattatatt cattcgtgtt tgtgtatgca atacatgtgg gtttcatatt
      781 aattttcaat aaaaggcact attatgaaca aagcatttta tagagttaga gaaaatcatc
      841 attctgttca aataaaaagt atgatctgaa tatggagaag ataagaacat ggtacgaagg
      901 aaacatggac aaggtatgga ggagatatgg acatgatatt gaagaaaaac aaccaacatt
      961 gactcaataa aaaaatcccc gccacaaagg atttgccatt attttcaagg tttaccgcaa
     1021 atttaagctt tgtaagtaat gtgctgccgt attgaaaatc taacttggta ttcaatttat
     1081 tatgggttat ttgtaaaaat cgccagttga aaatactttt ttttatgaaa aaattgccag
     1141 ttgaataact ttttttatat ttgcaacaaa aaaatagtat gaaaattatt agaaggatac
     1201 ttcaagataa gataatagat aaaatcgcac ccggtaaggt cgtgttgatt ttcggagcaa
     1261 ggcgtgtagg gaaaacgatt ttaatgcatc aaattctgga gtcttatcaa ggtaaaacaa
     1321 tgttactcaa cggcgaagac tacgatacgc tgacattgtt ggaaaaccgt accatagcca
     1381 actaccgaca tttgtttaat ggaattgata tggtagctat agacgaggct cagaacattc
     1441 cacagatcgg cagcattttg aaattgattg ttgatgaaat acccggcata agcgttttgg
     1501 cgagtggttc ttcgtcattc gacctgctca acaaaacggg tgaaccattg gtcggacgca
     1561 gttcgcaatt tttgcttaca cctttttctc atcgggaaat attgcaaatg gaaaccatgc
     1621 ttgaaacacg acagaacctc gaagctcggc tgatttacgg ttcatatccg gaagtagtaa
     1681 tgatggataa tttcgatcgc aagaccgatt acctgcgtga tattgtcgga gcttatctac
     1741 taaaggatat tttggctgtc gacggaataa aaaattctag taagatgagg gaactgttgc
     1801 ggctgatcgc atttcagtta ggcaacgagg tttcctacga cgaattaggg aaacagttag
     1861 gcatgagcaa aacgaccgtc gaaaaatatc ttgatctttt ggaaaaagta tttgttattt
     1921 atcgtttagg agcttattct cgtaatctgc gcaaggaagt aacgaaagcc gggaaatggt
     1981 atttttatga taacggtatc cgaaatgcca ttataggagc attctcgccg ctggctatcc
     2041 gacaggatgt aggtgcatta tgggaaaatt atctcatcag cgaaagacgt aaagcaaatc
     2101 ttaatgaaga attacacaaa gagttttatt tctggcgtac ttacgataaa caggaaatcg
     2161 atttaattga agaaagttca aatgatctga cagcactgga gttcaaatgg agaaacaaga
     2221 ttccgaaaat tccaaaagcc tttcaagaga catatcctca tgcaaagttt tatgtggtaa
     2281 ataaagataa ttatctggaa ttcatataat ttcaaggata tttccaacaa aaataagttt
     2341 tgaaaaaact acatctccgt attataatac attgacacat tttttcaagc gggtttctct
     2401 tttccgtata gcccataatt cggcgtagcc ttccgaccac gtcgtattaa aaataaaatc
     2461 tccggattat caatcattca acgaattaaa tgaagttcaa aaaataaact tcatttcaac
     2521 gaattgaaaa tttaccgaat ctatttcccc tttttctcaa taagcatgtg gatcttcatt
     2581 gatttcctcc acattggtac gctttttctc aagtgcccgc caagcataaa aaatataaat
     2641 taccacaaac ggaataatca gcgaaacaat ggacataacc ttcagggtaa attcactgga
     2701 agaagcattg gaaagggtta gcgaactttg cacgtgtgtt gctgaaggat acaacgcagt
     2761 attattaaag cccaccagca acagaagtcc cagcaccgcc agaacagtcc cagctcctgc
     2821 aaaccaaatt ccttttctgt aattttctga aaacagcgtg cgaataattc catacaaaac
     2881 acccactata cccagtaaaa atgttgtcat cactgccggc atttccacca gattgagaaa
     2941 atattggtga ggacttaaat atacttcacc tgaaacagga tgtacggcaa aaccatcgga
     3001 aagaaaagtc cagataacaa atgcaaggaa gaaaactaca aaaggaatgg cattgtacca
     3061 caagtatttt cgtgaacgtt tttccagcat ttcatgatcc acccgattga taaaatacaa
     3121 agccgccagt gtgcgcgaca gaaagaaaac cgccagtccc agacaaacat ttctcaaatt
     3181 gagcaaagct tccagtccgt gccacggatg ctgccaacgg ctgataatag gcgagagcgg
     3241 attggaaata tttgctttgt ccactacaaa atccgaaccc gtaaaaaacg tggcaatcac
     3301 cacgcccaat aaaaaagttc ccaataatcc attcagaaaa agaaaaaaac gatacgtatt
     3361 ttttcccagc agatttcccg ctttggtttg aaattcataa gaaactgcct gcagggtgaa
     3421 acaaaacaga ataatcatcc acacccagta agcaccacca aaactggttg agtaaagcag
     3481 cggaaaagaa gcaaaaatgg cgccgccaaa tgtaacaaga gtcgtaaaag tatattccca
     3541 tttccgtccc agaacattta ccagaagtcg tttttcatct tctgtttttg aaagcgaaaa
     3601 aatcatggat tgaccaccct gtacaaacaa tagaaaagat agaagacctc ccagcagagc
     3661 aatcagaaac caccagtaat gctgtaaaaa agaataagtt atcatagtat tctaaattta
     3721 aggaaagaca aaatgaattt tctcctaaaa aattactctt ctattttctc agcttccggt
     3781 cccttcttta tttgtttgac cataataccg atttcggcaa cgagtaaaga agtaaacaaa
     3841 atcaggaaaa ggaagaaagt cagtcttacc gattcggccg aaatgctcga cacggcaacc
     3901 tgaacgggca aaatatcctg aatcgcccaa ggctgacgac caatttcggc aaccatccaa
     3961 ccggcttctc cggctacata ccccaatggt atcgtcaaca aacaaatcca ttgtaaccag
     4021 cttaaatgct ctaatttttt tcgataagat aaaataagta cccccgcaaa aagcacaata
     4081 aaataaattc ccaaaatcac cataatatgg aatggataaa ataccagcga cagattcggg
     4141 atcaattgct gaggatggtc cagataacca taaccaaaat aggcatagtt tttcatgagc
     4201 ctgtccttat acattttggc tgcaaccgta tctttttttt ggcttgcttg ctgataatcg
     4261 gccagtgcct gaatggcctg tttaccgcgc tctctttttt cttcaaacga taatgtttct
     4321 cccgatgcag tttgatatcc accttcaata atatctttta atcccggcac aaaagcattg
     4381 gcatcgcgat agcccagaaa tgaaaggagt tttgggattt caactttaaa cagataagga
     4441 gcaagagtgt cgttgtatga ttttttctcg ggattcaaaa taccaatggc tatcaggcct
     4501 gcaccatttt tcccttcata aagtccttcc atagctgcca gtttcaccgg ctgacgctga
     4561 gccacattat aagccgagcc atctccggta taaagcagaa gcaaagaaga aatcaaaccg
     4621 aataaagcac tgacgcgaat gcttctcaaa gcgaaatcct tttcgcgttt attgagcaaa
     4681 aaccagcagc tgatacccac aacaaataat gcacccaata cccagcccga aataaccgtg
     4741 tgaaaaaatt tgttaatggc aacaggagaa aagagaatac tccaaaaatc aatcatttcg
     4801 ttgcgaaccg tatcgggatt aaatgtcatt cccgtaggat attgcatcca agcgttggca
     4861 acaagaatcc acaatgccga aatagttgcg cccagtgtgg ttagccatgt agaagccaaa
     4921 tgaaaatttt tccccacttt tttccaacca aaaaacatga tagaaataaa tgtggcttcc
     4981 aaaaagaaag ccaatatccc ttctatggcc agcggagctc caaaaatatc gcctacaaac
     5041 cagctgtagt tcgaccagtt ggtgccaaat tcaaattcga ggataatgcc ggttgctacc
     5101 ccaattgcaa agttgatccc gaaaatggtc atccagaatt ttgctgcttt tttccattct
     5161 tccttccccg ttttcacata aagagtttcc ataatcgaca taatcatgcc taaacccaat
     5221 gtgagcggga caaacagcca gtgatacatg gctgtcatgg caaactgtgc ccgcgaccaa
     5281 tttactaagg atgcatccag taataattcc ataaatttca gtttattgat tctttctatt
     5341 tcgatttgtt gataagttcc atgcgcacat ggttcgcctt gccggcatca tccctgaaat
     5401 gttcattaag atagtcggga aagaaaaaaa ccttcagaat gccaaacatg atgaacagtt
     5461 ttatcaaaac gattatccat aaggtttttc ccaccgtcat gtttttaaaa ccttcggaat
     5521 aaaacgaata aatatttttt agaaacccga ctctctgcat aaatattttt ttgacaaaaa
     5581 taacaatttg ttcagattta caagcaatag cctgattttt tctttcaatt tctttatcat
     5641 tcaaatttta gattaaaaaa attaaaatac gcacctgcaa acattcgtaa aacagtttac
     5701 tttactctat tttaaccgaa aatttgcaac agtatattag ccctcaaaaa aatcgagcag
     5761 tagaaaataa aaattattag gaatcagtgg gttagaattt tcttaaagaa aatatgaaac
     5821 ttagacctcc tcccggctgg tttttagctg agatatttcc tttgtgaaat aaaactgcat
     5881 tttttacaat ggccaatccc aaacccgtgc cgccgaattt tctacttcgg cctttgtcca
     5941 cgcgatagaa tctttcaaat agtctgttta aatgctcttc agctacacca cgtccattgt
     6001 ctgaaaatga gaaataataa aactcgttat cttcacggta acaatttatc tcaaccgtaa
     6061 tattatcacc accataggtc aaagcattgt ctattaaatt gcgaaaaata gagtacagta
     6121 aagaattatt accgtttatt ttcatgtcgg aaggataatt ttttataatt ctgcactttt
     6181 tttgctcgat ttggacatgt acgtcattga gaacttcatc aatgacttca gaaagggagc
     6241 acggttcttt tgcaaacaat ttgtatgctt cgtcgagctt gttgagggta gatatatcct
     6301 gaagcaaagc atttaatctc tgggtttgct gataagctcg ttcgataaaa aaacgttcac
     6361 gttgaggtga gatattggga ttctcaagaa tactttccat ataacccata atgctactta
     6421 caggcgtttt cagttcatgg gagatatttt gtgtaagatg ccttttaagt tcattttcat
     6481 gttcctgggc agaaatatca tttatggata tctcaaacgt gtcatcctga aatacaatac
     6541 actgaacgta aaaagaatgt ccattttttt ctatcgtaat ccgttttcgt tttgtttcat
     6601 tgttagaaaa gcaatcgtca ataaaagcat ttatttcgga aaattcggaa atggaaaaaa
     6661 tttgatcaga acttttaact tcctgatcgg aaagaatatt ggtatattga ataaaatgtg
     6721 tgtttgccag aatttctttt ttgtcggaag agaaaatgcc caatccctcg cgtgagattt
     6781 gcaagtgctt aaacagtttt tcccgttgac gatcggcatc agctttcgat tccagcatac
     6841 gtcgatagat ttgaacaata ttattgctga tttcgcctaa ttcatcattc ggaaactgaa
     6901 tgtcggtatc gagaatatcg tcttgttcgg ctttttgagt aaatattcgc aatctgtcga
     6961 tagagcgtga aaaatttttc gaaatgataa acaatgcaaa aactgtaata acaaacatga
     7021 ataacataaa atacaagaaa aacatatttg ccctaagagc ggcaatgaga ttgacattat
     7081 aagggagagc gcaacgtatg taatattcag ggaaacgttg ggccatatag taatagtttt
     7141 ttcctgtgga agcagaataa cgtatggctt ctccaatctt ttccttgcgg gcttttgcta
     7201 tttccgggcg gtccaaatga ttttggaaaa gagttccttt gggcagaaag gaatcaaaaa
     7261 ttacattacc caaagaatcg acaatggtta ctctgagtgt gctgtcagga aacaggcgaa
     7321 cgaatctgct gagttcttcc caagtcggtt gttcactttg aatgaagtta tggatggtat
     7381 gcacgtagct gttcagttgt ccgtccagtt gcgatctgct gaattccttt tcgcgatgat
     7441 actgaaaata ggtaactatt acaaaaagga taaaaaatat actgaaaaag tataggaaaa
     7501 tttttttgtt tagtttcatg cagggtcgtc gaatttgcaa ggaaaagagt taaaaaaaca
     7561 cctcatctaa aaccaaataa aatcatctgt ttatccctca aaacaatagc cgtaacccaa
     7621 ccgggtaacg atattttttc cgtaaggtcc gatttttttt cgtaatcggg taatatttac
     7681 atcaatggtg cgatcaagca cacaaacatc gtctgtccaa acctttgcaa gaatctcttc
     7741 acgcgaaaat accctgtttt tgttttccag aagcaatttt aaaatttcaa attctttctt
     7801 tgtaaatgga atttcatttc cttccagcga tacttttttg ttgttgagat gcatttcgag
     7861 tttttcaaaa gtaagaattt tatttgtggt ttcatccatt tgcatcgact cggatcttct
     7921 cagcaccgct tttactcttg ctataacttc ccgtaaagaa aatggtttgg aaatatagtc
     7981 gtcggcacca atattaaatc ctgttatttt atcgttttcc gtgtcttttg ctgtcaggaa
     8041 tataatggga atagaagctg ttttcgggtt atcgtgcaac attctggcca ttttgaaacc
     8101 cgagatttca cccatcataa catcgagcaa aataaggtta taaacagtta aatccttttt
     8161 taaagcttct tcggcagaat atgccgtatc tacttggaaa ccttctgttt ccagattaaa
     8221 ttgaagaatt tcacacaaat cttcttcatc atctactact aaaatacgtg tgggagcaat
     8281 cataaatttc tactttagaa ataataaatt aaccaattta tgctcacttt ggagcaaaaa
     8341 ttttattcat gttgcaaagt aactgaagaa ttatttcgat attatgtaat atatattaca
     8401 aatatatgac aaaaattgaa ttctctgact aaaatagttg aatttagaat ttggcataat
     8461 tatactttta ccgatggaaa atacaatcga ttcgtatatt ggactttaaa agaggaaaca
     8521 ggaattttcg ggaatgtaca tattgaaaaa ggcaattcga aaaagaactg cctttgcaaa
     8581 aaagatttaa aaaaaatgtc aatgttgtgc caacttaatt tgaagtgaat tggatgttga
     8641 cagatctatt tccacatcct gttcttcgta tgaaatcaaa cttgctttca atcgagcttt
     8701 cccggttgga aatgtagaaa ttgtaaaacg accatccaaa tcggtataaa ctttttgacc
     8761 attcagcatt atttttacgc ctgccaatgt ttcaagggta tttttatcca ctaccttacc
     8821 ttcgataaca gtttttgtgt tcgaaacatt ttttgcttcc gaagaattat tctctccggc
     8881 cataacattg cctccgagaa aaaccgacaa tgcgaaaaaa actacagcaa aaaaattttt
     8941 tttcatttga tttgtatttt aatttgtttg aaattaatcc cctttgtttg gatacaaagt
     9001 aagttactgt atatgacaat agtattacaa aaaaaataca aatacatgaa aaaattaagg
     9061 ggtagaatag tttttttcgg aaattaattt ccctttttcg tcccacataa accacgttcc
     9121 gattttttcg ccctcatgat aatgcatttc atagcgcatg ataccattat catcccatat
     9181 atgccaagta ccatgttttt tgtcgttccg gtaaaaagct tcagatatca gtactccaga
     9241 ttcgttgtat gtgcgccatg ttccgtgaaa ttttccgttc cggtaggaac gtatttcgtt
     9301 gggcttcccg ttggggaaat aaataatgta atctccttcc ggcttaccgt ccttgatgta
     9361 catttcgagc cgcaaagatc cattgtcgta aaactcgcga taatcccctg tataaagttt
     9421 ggtttgggac aaatcggtga aaaacaaacc atcgctttgc actatggtag atggttcgct
     9481 tttttgtgaa aaaacgctca tgtggagagt cattattacc gacatcaata acaatatttt
     9541 gtgcataact tatatttttt atcgttaata cctctacaaa tgtaagtgaa atatatgtga
     9601 aaaattttca tcatcaaaaa atttaataca tgttacaaaa aacagaaaaa atttctcaag
     9661 ttttagtaga aagcgaaaat gacttagcaa gataaaaata ataagtgtct tatgaatttt
     9721 tacttgattt aaaattaatt gaagtttaaa ttttcttcaa tatggtgtcc atcacccaat
     9781 tggttcgaat ggccgaatag caattgctta caggttcagt gcctttcccg ttcaaaaaat
     9841 tgataatact ttctatcaaa taaaattgaa tgttttccgg ttttggttca gaaaattgtt
     9901 ctactttttc tccattttca agcactattg gactaaaatc gaaagttgaa caggtgagtg
     9961 ttgcctttgt tcctacaaaa agaatttcgt cacaccgaaa attttcggga gcagaaaaag
    10021 tccacattcc gctacctaaa atcccgttgc taaagcgaaa aattccgctc accgcatctt
    10081 ccacttcata cagaccggca atgttcgaag taaaaccctg cgcttccgaa ataggaccaa
    10141 gaataaaatc gagcaaatcg aattgatgcg gagccaaatc gtaaaaatat ccgccgccgg
    10201 caatttcctt cttaacccgc caaggcaaag tgtcgggacg aaaatcattt tgcgacggcg
    10261 gtagggcaaa ccgaatctgt acggaagata tttttccaag ttttccctcg tcgatcaact
    10321 ctttaacacg taaaaaataa ggcaatgttc gccggtaata agcaacaaaa caaggcacat
    10381 gggtttcttc ggcaacttga acaattttct gaccttccag ccaggaagaa gcaagaggtt
    10441 tttctacata aacaggtttg ccggctttca tggctttgat ggcatattcg gcatgagaag
    10501 aaggaggcgt tgccacataa actgccgtaa cttctggatc gtgtatcagc ttgtcgggat
    10561 cgtcgtacca ctttttcact ccatgacgta aagcgtagtc tttggctttg tccagacgac
    10621 ggctcattac ggccacaaca tccgaaccct cgattttctg aaaagccggt ccactttttt
    10681 tctcggttac atcaccgcaa cctataaatc cccaatgtac cattttatga aatttgatat
    10741 tcggtgtaag gtagttccaa tgactcggct acatttttaa aaacaatttt gccatcgaca
    10801 atgtttaaac cgtgctttat atcttcgtga tttttgcaag ctttctccca accaagttca
    10861 gccagcttga taacgtaagg caatgtggca tttgtcagtg cgcgggtaga agttatggga
    10921 accgcacccg gaatgttggc gacgcaataa tgcaatatgc cgtccacttc gtaaacagga
    10981 tcggtgtggg tagtcggatg agtggtttca aaacaaccac cctgatctac agatacatcc
    11041 accataacgg ttcctttctg cataagtttg agcatttctc ttgtaatgag atgcggcgct
    11101 ttggctccaa ccgtgagtgc tgcaccaatc attaaatcgg ctgtgggtaa aagttcggaa
    11161 atggcatatt tgctcgagta ttgagttatt acattggcag gcatgacatc gtcgagataa
    11221 cgaagccgat acagattgac atcgagcacg gtaacgtttg ctcccaaacc ggcagccatg
    11281 tatgcagcct gagttcctac tacacctcca ccaataatga gcacattggc tggacgaaca
    11341 ccgggaacgc ctcccagcaa aattcccttc ccgttttgtg gcttttcgag atatttggcg
    11401 ccttgctgaa cagacatgcg ccctgctact tcactcatcg gcatcaatag cggcaaactt
    11461 ccatcttttt ccattacggt ttcgtaagca atacatacag ccccactttg catcatggct
    11521 tcggtaagtt ttttactcga agcaaaatga aaataagtaa acagtatctg attccttttg
    11581 ataagagaat attccctttc aattggttcc ttcactttca caattatatc agccatgctg
    11641 tacacttctt caatggtatc caacataacg gctccagccg ataaatacgc ttcattggaa
    11701 aatccgctct cttcccctgc tcccttttgt acaaaaactt catgaccata tcggtgaacc
    11761 aactccataa caccggccgg aaccattgcc acccggcctt cttgcggttt tatctctttg
    11821 ggaactccta ttttcataat cttttatatt taattagcta tttgataaca aacagtgtac
    11881 aatacaaaca aacaacttct tctcaccaaa caatttgaaa taattttgtg aacattaaat
    11941 cgaaaacagc aacaggccta atgaagccga caaaaaggca cttaaataaa tgaattttta
    12001 acttttttgt aactttcatt ttgttttaaa acaaatcaaa atcatttccc cttttctatt
    12061 aatgaaatgg aacttcgatt cggctacaat agagattcgc cattttcgct tatctgtttc
    12121 cgtaaaactt tttatttatc cgttttcttt ttccttgctt tcaaatttga gctgatttga
    12181 ttttagctga tggttaagtt ccctgttttt gtaaacatcg catgccagat acaaagcttg
    12241 tcggaaagaa ttttcggaag caacattctg tccggcaatg tcgtaagcag tgccatgagc
    12301 gggagaagtg cggataatgg gaagtccggc agtgaaattc accccattgt ccatagaaat
    12361 tgttttgaaa ggtgtcaggc cttgatcgtg atacatggcc aaaatgccgt cgaactttgt
    12421 aaaacttccc gatccgaaga aaccgtcagt tggataggga ccgacacaga ttattccggc
    12481 cttttcggct tccttcatgg cagggagaag aatagttttt tcttcgtcgc caatcaatcc
    12541 atcatcgccg gcatgtggat ttagtcccaa aacggcgatg cgtggtcgaa caaccataaa
    12601 atcctctttc agtgagtggt tgagaatttg cagttttttc aataccaatt ccgaagttaa
    12661 agtggacggg acctcgcgta gtggaatatg ccccgttaca acagctatcc gcatcacatc
    12721 gttgaccatc aacatcagcg cattttccgt gttcccgaag tgctgggcaa gatattcggt
    12781 atgaccggga aagtgaaatt tttccgattg gatattcttt ttgttgatgg gtgcagtaac
    12841 caacgcatgg atttctccat ttttcaaatc ttctaccgca cgttcgaggg caatataggc
    12901 agcttctccg gcttcgggtg tggattgtcc aacttcgacc ttaatatttt catctacaca
    12961 attgataata ttaacccttt tgtggttcaa ttcttttacc gaaccaatga tgttgagatt
    13021 cagattttga atatccagca tttttctata atatccggca gctttggacg aaccataaat
    13081 aatcggtgta aaaaagtcga ggataacagg atcggacaat gctttgagta tcacttcata
    13141 acctattcca ttaaaatctc catgagtgat tcctaaaata attttttgtt ccatattcta
    13201 atgtaaaaat cgaaaattta tttttttggt gttaccgata tttcctttaa tgcattgatt
    13261 tcctgtggta atttagctga gtaaacaact gattcgagct gacgaatggc attccttttt
    13321 ttctcaccag gagcaaaaac aaaaccttcg atagtaatta cccgctgatt aatttcatcc
    13381 aaacgggtat gactgtagaa tggacctccc atagctgcac catgaaacat tttccataat
    13441 cctctaatct ctgcacaata accaccattc acccagattt ccctgaagat tggtggataa
    13501 tatttgtact cggttcccat ataagagcct tctatttctc cctgaacatt atatttcatt
    13561 accgaatccc gttttgccaa taagtaatct tttgtaaaag tgtttttgtc ggtatatgga
    13621 taggagtata ttactatatc tttccttatt ttcggattat cgttcgtaat ccagtaaaaa
    13681 tccttttttt taactgcttt tttaaattcg ctaggaatat cgatctgaat accaaaaatt
    13741 ttttctattt ctgctctagc ttctaaattc acaaaacttt tgttgtattc catttgtctt
    13801 tgtcgttcct gagtaataaa aaaatcaaga atatttttcc cgaatttttg cacagcttca
    13861 acaaacgaac tgtcattggg tgccgtaatt ctgacaatgg cttgtggatg agcccattta
    13921 tcacgtgcat acactacttt tggttgagag tatttttcag aaatatcgga aataataata
    13981 tttctcgaag acttgaacat attggagaac ccttccggtc gacaaggaat aatatccatt
    14041 acaggctccg gttgcggtag cgcttccatg tcttggtcca acaagtcaac caacgtacga
    14101 cctggtgctg aaatccactg ttgatgatcc attacaacca aaacttcgta cattgaaccg
    14161 gtaattcctg gcaaggaact tttattggaa ttgcaacctg acatgattaa aagtagagtc
    14221 agaacaaatg ttgtttttgt tttggtcaaa ataaaattaa atttaagagt tgaaatattg
    14281 ctcacgcgaa tccgttctac tattcttgcg tgaaatctta agaatgctcg tttcatattc
    14341 ttttgttttt aagtttaaaa aatcgcttaa atttattggt ctgtttttga tatcttttat
    14401 ttatttggga cattatttgt cttcatggtt ttgcaaaaaa atccagaact gacttcatga
    14461 aagcttctct ttctgcatct gaaacgatgg tttcaaaagg aaatcctaac gcacaaattt
    14521 tatatttccc cgaataaatt actcctgcac tcagattgtt ttctgcataa cgacaaaaag
    14581 tataagcatc ccttgcagcg ggttcaatgg catcaggtga ttcgacaaaa taagaatatt
    14641 gattgggttc ggagtaataa gaaaattgat gtcgaacaaa ttgtttaaat ggagaagata
    14701 caaattttac gttccccgta atagccgctt gatgtacccg atatttgatt ttaagagtgt
    14761 tttctaaaaa acgaatatcg tctggtaagg cgtcatttct gtcaaacata tcagacgcaa
    14821 ttgatgcccc tgtaaccagt agatttccac cttgctgatg acaaaacaaa ttaatttttt
    14881 gctgtaaagc taatggaaaa gttttaaata aagaaatatt ttctctaacg ccaacatcag
    14941 tctgcttctg ttttcccaaa attaaatcta cagtctgaaa atcgttcaaa tttatatcgt
    15001 cattcataac agcttccaca ctgcatgaaa caaaagaata accggcggca caaatagctt
    15061 ttccatgcaa aaaagggtaa tcaaaggaat ttcctgccag aatttgggtt tcataattgg
    15121 catgactagc tccgaaaccc ggtgcatcat cgtcgaccca ttttctatca cgtctaaatt
    15181 catattgttt tcccgtaaag ttatagccat ataaataagg tacacctgca tccatgtcgt
    15241 tcaaaaagcc tgcataggtg gtatcgataa caaaaccggc aggcggagct actctttcaa
    15301 aaccatttat gatgagaacg ttgccttttt cgtgcggatg agagcatacc gacaaaattt
    15361 ctgaaggaaa actctctcca ccacgattta cggatgttac tttaaaactg tatattttcc
    15421 cttgttccaa caccttaagg tattcggtat ccgacacaag taccccatta tcaaagtcac
    15481 catcgtcaat tctcgtgtac accacaaaat gttctgctac agcactaggt tctaatgaat
    15541 ccattcttgt tttccaccga agtttaactt tgtttttatc tacaaaaatg cagctaaatt
    15601 gtcgtatggg taagggctgt accatataat cctcctcgtc attcaatgcc gacaggtatt
    15661 ttaaaattcc tttgtaaatg gagcggctta ccaaaaactg aaaacgggga tcgagtccta
    15721 atttcatgtc tgcataattt tgatgcgaaa gtaattcgag caacatagtc ggaacttcag
    15781 gaactctggc ttcatagtaa gatttattcc aaagtccgcg tcttatccaa ctatcattaa
    15841 agctcatgcg tatgtcttct acaatttgag tttgaataat atcggtcaaa tcacgcgagg
    15901 cccaacgcga tactttatta ttataggatt tcccattacc cttgccgact gtgaatattc
    15961 ccaaagtgcc aattacagaa tcgtttttgg ttgttccggc atcggtatga agagccaaag
    16021 acaaatccaa tggaatcttc aatccttttc ctttgggatg gagcgaagaa cctgccgtga
    16081 gaaaatttac ccatttagct cttgactgaa agtcatcaat ataatcgttt gctccgcgat
    16141 tggggctgta gatgctgtcg gggaacccag cccattgtaa ccaataccgt gctccttctg
    16201 caaagcgagg ataaccgctt gtttcgggtt cagtctccca actcaatacc ttttctttct
    16261 ttaatgcagg attttttatt tcagaacttt tatggttttc caatattcca ttctgatctg
    16321 gagaacgggc aatatttccc attcctccac caaatttcac agcatcggct gttacaattt
    16381 cttctttttc ccctgtaaaa ttcgatagta ccactttgcc gttttcattt cttcctttgt
    16441 cgaaattaaa ttgtcctaga taaatccaag ttccgccacc catagtctga ttcacggaaa
    16501 atttggtttt acctcccttg tggtagaccg tatagtgagc attttcagta ctttggggta
    16561 gtgttttata agaaacataa atgccatatt tccctgcttt aggaaaatcg gggatatatt
    16621 caacaaaact tgctttgttc tctttttcta ccgaatgtat ttgacggtaa cttcccatgt
    16681 taaaaggatt ttcgccttgt aaataaaaac ttttgaaatt tgcaaatcca aaatcttggc
    16741 tttctgtcca aacttcttgt aggttaaatt cccgatatga gagtgaatct ttattgttat
    16801 tgtctacaat aacttcattg ctttgcatat ctctttcgcg tggtgttaat acagtggctc
    16861 cggcattttc aagcataggt atcagatacg gataaacaat ggatagggta aaaagatctt
    16921 ccactgtttg catcacacgg gcacgctgcc actcccaacg agaaatgctt tgttcgtaat
    16981 acaaaccatg actagcccag agagcaatat tttttctttc taatccgttg gttacattat
    17041 atggttttga aagatttttt accaatgaat actttggttt ttttggatca aaaattctgg
    17101 tttcgtctct ttttcttttg gttcgataaa aatttggaat taattcttct atagtttgac
    17161 cgtcgaccat acaaactatt ttatagtttt tgtaggacgg actaaaaatt tgtttgagta
    17221 cccggtagat atgttttaca ttttcttctc taaaaggcat ataaccaaac ttttcattgg
    17281 ctttgataat aatttttttt tctttattgt tgatataaat attatctacc gatattttct
    17341 ccacaaatgt atatttattc gcaatagagg ttagggagtc tgaaatttgt ttttctaata
    17401 acgattgtgc aaaaacgggt ttaggtaata atcccgctaa aacaatgata aatagcaaaa
    17461 tcgttttttc agaaatcagg tgaaatttat ttttattgat tatttttctc atttgaaaga
    17521 ttggttatga cattttttta ttttccaacg tagaaagcag tccgcaggca gctgaaatat
    17581 cttctcctct ggatttacga atggtagcca aaattccatt attattcaga taatcgcaaa
    17641 accatatcat ccgttcatcg cttgaacttc ttaaagaaac atttggtatg gcatgaaatc
    17701 ggattaaatt tacccgtgcg ttcatgcctt ttaaaagtct taccaattct gaggcatgtc
    17761 gcatagtgtc attaaactta tcgaacatga tatactcgaa agaaagtttt ctttgccgtc
    17821 caaaattgta atttttaagc acttctacca cttgtgaaat gggatatgct ttctctatgg
    17881 gcattagttc gagcctctcg ctatgaaaag gagaatgcaa actaatagcc aaattgcaat
    17941 tggatttttc caaaaaagtt tgcatgccgg gtattaatcc tattgtggaa accgtaattc
    18001 ggtaggggct ccatgcataa ccataattgg cagttaaaat atttaaggta cgcaataatt
    18061 ctactgtatt gtcaaaaggt tcgcccatgc ccatgaaaac aagattggta attttttcag
    18121 cttcaggaac agaaagtatc tgatttagaa tttccgatgc cgaaagttgc cccatgaatc
    18181 cttgtttccc agtcatacaa aacacacaat tcattttaca tccaatttga gaagaaacac
    18241 acaaggtatt ccgatgttct tcagggataa aaacggtttc gataaaatga tctttttctg
    18301 ttcgaaacag atattttttc gtttcatctt ttgatactgt aactttaaca ggagccaaac
    18361 ggccaacttt atatttttct tttaacaggt ccctgttttt aacggaaata tttgacattt
    18421 cattaatatc agctaccctt tttttataaa gccagtctga aatttgtttt gccgtgtaag
    18481 tgggcatttt cagttgtgaa acaatatcgg taagctcctc caacgaagct cctaaaagtg
    18541 gaattggcat agggaaatat atttttaatg agatagggaa taataaatat ctgtttttat
    18601 aacaacaata attatctaaa cgttttatca gacatttgtt gccaatgcaa agttaaaaaa
    18661 actgaccata aaaaagaaaa acggaatgaa agagtttcat cccgttttta caatttttat
    18721 ttaacaatac ctttttaacg aaatattgat tgaaaaactc ctcaatacct caataaaagt
    18781 tggaacggta atcttcaatt ttagtccttt ctctcaaatt ttctaaaatc aaatagggca
    18841 aagaataaag cattcttgca tttagttgaa ttatttcctg ctgagcattg aatgtttcgg
    18901 tattttcctt tttgtttgat gttttcatta cataaactcc agcatttcct tgaaatggtc
    18961 ccgaaacttt ttcgagtgga acaatcgtag cttttcctat gatgtaaggt tcaaatccgg
    19021 ctaatccaaa ctgataagaa gtaaaattaa cgttttcggc cattttaatt tctgcattca
    19081 gtgaagcagc taaagcttcg aggtcaggtg ttttctttaa ttgctctgaa atattattta
    19141 tcatttgcgc cgccttttta tctcttatta tttcgctttt caactgagca gaaacttttt
    19201 ctatcggcat ataacccttt tcgttttttt cggttatcat cgccactaca taatagttgc
    19261 cgcaatcgaa aacatcagaa acatcgcctt ttcccccttt aaaggcccag cgaactattt
    19321 gacgggattg gggtaaattg tttatttgtt cggtattttc attcagattt tcggtttttt
    19381 gcacaatata acctttttct tgggctcttg aaacaaattt ctctgcatcc aattcggctg
    19441 caaattgttt ggcttggttg tatatttgac tgtatgtttt actacttggt gtaacttttc
    19501 tttgaagaat ggcaagtttc acttttctgc gtaggggcgt gcggtcagtt acctgcatga
    19561 tttgagttcc ttgaatatct tttattacaa aaacttcatt cagaggcttt ttaaatgcgt
    19621 tttccaaaat ttgcttatcc actccttgag ctccgtcggg taaccagcct atttcacctc
    19681 cgtttgcagc agtttgagga acagcagaat actttctggc caaagttgca aaatcggcac
    19741 ccgctcgaag ggcttttact atgctgtcag cttttccttc gttttcctta gtcaggaaaa
    19801 tatgccgtaa cttaactgaa tcgggacgaa gaatatcggt ttccatgatg cgcgccatag
    19861 tgtaagtgtc gttttcaaaa acaggtccga acacatcatt ttttttccct ctaaatgcaa
    19921 aatcttttaa catggcaggt acagttgctt cagaataata tctgccgtcg tacataatgt
    19981 cggaattggt atttaccaga cctaccacat cgtctgttgt tctaaattcg tcttcaagct
    20041 tgttaatcca ttcctcagct tcttggaaat cttcttgtga aggcttgatt tccagggcaa
    20101 cataattgat agatgcagat ttttcctgtt tgaaaagttc ttttcgtttg ttataaaggt
    20161 ctctaagttc tttagaactt actttaaaaa tagaatcagg cactgaataa tacggttgca
    20221 ttacataagc cgcgtcaaca gtaatttttc gagcttcaaa attcattttt gcatctatac
    20281 tgttggcaga tatggcagaa gtaattaact tattgtattt ttcctgtaaa atagcatttt
    20341 ttacgttttt ttcccaaaaa agccagtaat ttttagcttg atctatttgt tgcttcatct
    20401 cattattggt aggagtaaca tcaagactgt tgaggaattg gacaagtaaa gagcgactaa
    20461 attgtcctgt ttcatccata aaagccccgc gctgtagaat gataggatgt atgtttttcc
    20521 cgatcagtct ttcagaaagt tcttcgggac ttacggaaag ccctattttt tttgcttcgt
    20581 gagttaaaat tttttccatc agcatgtttt cccacaccga tgctcttaat tgggatacaa
    20641 cttcttcgtt caaattcgtt tgccctgtct caattttata cacttccgtc atctggtcaa
    20701 tggctgcact gaactcgtgg atgctgatgt tttcatcacc tatgacggca attttttctt
    20761 tcgactgatt aaaaaaagtg gagctcgaat taagaaaatc tccaacaata aaagccaata
    20821 aagcaagtcc gataactaca ataagcaaaa ctcctttgct cctgatcttc tctaaagttg
    20881 ccattttaca tttaaaatta tttgctataa aactattatt ttttcaattt taagggcacg
    20941 aatatacgaa aaattcttga tactttgatt tttgattgaa tttcttgact tcaaaaagac
    21001 aaaaaaatta tttctgtagt ttcattttta ccagttcaat acgattattg gtaattttta
    21061 tacatttgaa agtataatta tcaatttgga tggactcgtt taacttagga aaatgctgat
    21121 gatgataaag aataaaaccg gcaatagtat cgtaagcatc cgattcgcga aggttgagat
    21181 gaaatttttc attaatttcc ttaacttcca ggcggcctga aaaaacatat tcgttttcgc
    21241 cgaccttttc tgcaacaaaa tcatgtgtgt catgctcatc ttctatttct ccaaaaattt
    21301 cttccataat atcttccaat gtaacaatac ctgccgttcc tccaaactca tccaccacaa
    21361 ctgctatact ttttttctct ttcagaaaaa ttttcattag tttttgagct gccatgtttt
    21421 cgggaacaat gggtatctgg ttaatatgct ctctccaatc attttgatgc tgaaacattt
    21481 cagaagaatg aatataacca atgatgttgt caatattttc tttgtaaata ggaattttag
    21541 agaaacccgt ttcaatgaag gtttgcttta acgtttccaa atccacattg taatccaatg
    21601 caactatttc ggtacgtgga accaagcaat cgcgtaattt aacctttgaa aaatcaagtg
    21661 cattctgaaa aatttttact tcgttgtcaa catcctgctt gtgttcggtg ttttcaaaac
    21721 tttcgtgtac caaataattc aaatctacgc gagagaaggc attttcatta gccaaagggt
    21781 tgatttttat acccattagt ctcataattc caaacgaaag agacgttgct aacaaggtaa
    21841 tggggtataa aattacatag aagaacaata acaaaggaga aaaaatgctt aaccaaaaat
    21901 ttggatttac acgaaaaata cttttgggta aaaattctcc cgtaaccaaa acaagagctg
    21961 tagccactat agtttgtgtg aaagtaatta aaaattgact accaagtatt tgtagcaatg
    22021 gggacaaaat ttttgccatc agcaacccgt agataacaag agatatatta tttccaacaa
    22081 gcatggtaga aatgtactgc tggggatgct tataaaaaat agtaagtatg gatgaagtta
    22141 tactgcgctg tttcttatcc agctcaaatc gaagcttatt tgaagaaaca aaagcaatct
    22201 ccatcccaga gaaaaaagcg gagaagaaaa gagttattaa aacagctgta aaattattca
    22261 ccttaaaaaa ttagctttac acttgcaaag atacaatatt taaagtaaca tacagagcgt
    22321 ttaaataaat tataaaaaaa gaagccactc ttaaaacctg attttttttt caaaagggag
    22381 aaaaaatgag attttttttg aagagaagta ttaaattttt tgttgtttca atttatcaaa
    22441 agttacacaa taaataacaa agaacatcaa tagacgcttt ttttcaaaaa aagtctattg
    22501 atgttcaaaa ccagaggaaa aaagtcaatt aactttttta atcaattcca ttcctatttg
    22561 caaagctttt tcgttttgcg gaatcaaatg atgatgacgt tcgggcaacg attttttcaa
    22621 accttccagc acattctcta tcttcacaat gggcctgact tttaaaaatc ctcccaaaat
    22681 aatcatattg agcgttttgg cagcattatg agctgttgca tattctgttg catcgattcg
    22741 atacacgttg atatcggtgc gcgtggggaa tttgtgaaat ccgtttccgt caaatatcaa
    22801 ggtaccgccc ggtttcacct tgctttcaaa tctttccagt gaaggctgat ttaaaatgat
    22861 agcagtatca taggagctca aaaccggcga gctgattttt tcatcgctta aaataaccgt
    22921 aacatttgct gttccccctc gttgttcagg cccgtatgat ggcatccaac tcacttcctg
    22981 gccttgcatc aagcctgaat atgcgagaat tttccccatg gaaagcacgc cttgtccacc
    23041 aaaacctgaa ataatgattt cttctttcat ctgtttattg ttttttcaat aatactttct
    23101 cttttaaaac aaataagttt accttaaaat caatatgatt tattgttatt attctaattt
    23161 atgatcagtg tagaaattcg gtatttaaag agtattgaaa catcgattaa atgtccttta
    23221 aatctcccag cggataggca gggaacatat tttcttccat ccatttgttg gcattttcgg
    23281 gtgtcatttt ccagcctgcg ttacaggtag aaactacttc tactatggaa gttcccttat
    23341 ttaacatgga attttcaaaa gctttctgaa tggctttttt tgcttttcgc actccggcta
    23401 ccgactgaac gctttgacgc gtaacatagc aagttccggg caattgtgcc aacaaattgg
    23461 tgatattcag aggataacca ttcaattccg ttttccgccc atagggagta gtagaagtta
    23521 ccatatccgg cagtgtggtt ggtgccattt gtccacctgt cattccgtaa ataccgttat
    23581 tgatgaaaat gatcacaata ttttcaccac gattacaagc atgaatcgtt tcggctgttc
    23641 caatagcagc taaatcgcca tctccttgat aggtaaacac ataacgatcg ggcaataatc
    23701 ttttaatggc agttgccaaa gccggtgctc gtccgtgagc agcttcctgc cagtcgatgt
    23761 caatataatt gtaggcaaat acggcacatc ctaccggagc tattcctatg gttttatcct
    23821 gaatgtccat ttcatcaatt atttcggcaa taatccggtg aactactcca tgactgcagc
    23881 ccggacaata gtgcatataa gtttctttga gtgatttggg ttttgcataa accaaattct
    23941 caggactaat tatttcgttg ttcgacattt ttctaaattt ttgaatgaat aatactgtta
    24001 attaaacttc agcaaatgaa ttaaccggct gaagaattat ttttatttga tcagtttgga
    24061 taccaaagca tcgtaaactt catcgggtga aggtactatt ccgcccaatc gaccaaaaaa
    24121 ttcaacggga acttttccat taactgccag acggacatct tccaccattt gtccggcatt
    24181 catttcgata gaaatcattc ccttgaccga cgaagccaat tgattaataa tatcttttgg
    24241 aaaagggaaa agggtaatgg gacgcagcaa accggccttg attccgttct cgcgtgccat
    24301 ttcaaccgct ttttgactca cgcgagcgca agttccaaaa gctacaatca ggtaatcggc
    24361 atcatcagcc atataagttt cgtaacggac ttcatttttt tccatctccc gatagcgttt
    24421 ctgtattctc aaattaatgg cttccattgc ttcagcttcg agcgccaagg aagaaattac
    24481 attttgtgat cggttggatg gcttacccag cgtcgcccac ggacattgtt cacgaatttc
    24541 atcgtcggtg cgacgtggtt ggaaaggagg caacaccact ttttccatca tctgaccaat
    24601 aattccatcg gccagaatca tgacgggatg cctgtactta aaagccaaat cgaaggacag
    24661 cggcataaaa tcggccattt cctgtacgga agccggagct aacgttagaa gtttataatc
    24721 accgtgtcca ccacctttta ctgcctgaaa ataatctccc tgactgggtt gaatcgttcc
    24781 caaacccgga cctccgcgca ttacgttaac aattacgcag ggtaactctg cacccaccat
    24841 ataggaaatt ccttcctgca ttaaactgac accgggactg gatgaggaag tcatggctct
    24901 ttttccacaa cctgcagctc cataaaccat gttgatggaa gctacttcgc tttcggcctg
    24961 taataccacc ataccggttg ttgtccaggg ttgcagttcc attaaggttt ccataacttc
    25021 cgactgagga gtaatgggat aaccaaaata tccatcgcat ccacaacgta tggctgcatg
    25081 ggcaatggct tcgtttccct tcatcagaag aacttcttct ttgttatctg tttttgagtt
    25141 tttgttcatt tttttacaat ttaaacattg aataaatacg gttgaagagt agaaatttat
    25201 tgtactctca ttttataaac cgtgatgcaa gcatcgggac aaacataggc gcaagcaacg
    25261 caaccaacac atgcatcagg attactcagg aaagaatagt tgtatcctct tgaatttgcc
    25321 tccttcgaca gcgataaagc tttgggtggg caagctacga cgcacagatt gcatccttta
    25381 cagcgttccg tatctacgac cacagttcct tttattttag ccattctgtt tatattttaa
    25441 attttttttc ttcgcaaaag tagtgaaatt tgagaaaatt gttggaattt ttttatccaa
    25501 attcttcaaa tgaaaaatat ttttaaaaat tttaaaatgt aattaattga tttttagtta
    25561 tttcttttgg ctgaacaacc agcttaaaaa atcaggttga gcaaaagcaa aatcccaact
    25621 gttatgtttt actccgggaa attcaatata ttcaacttta gtcgatccaa gcgcttttaa
    25681 ttcaatataa gcattgcgcg aatgttctgg agaaaccaca tcatcgctgc caccatgata
    25741 aattctgatg ggcatgtgtt taactttctt caacctttcc aaacttacac ctccacaaat
    25801 aggaatggct gctgcaaacg ttttgggctg acgacagatc aggtcgaatg ttcccattcc
    25861 tcccatggaa agtcccataa cataaatcct tcggctatct acagcctctt ctttcctgat
    25921 ttgtttaagt aaccgaataa ccatttgcat ggatggtgtt ggctctttgg aatttatgaa
    25981 agaaaatccg ttgtcccgtt ctttaattgg cgcccaaaaa ccattttcag gacattgcgg
    26041 aaaaactaca atggcgggat atttttgtcg gttctgtgaa tcagtaaaaa gggaagcgcc
    26101 atgcaccagc tgtttttcat tgtcatttcc tctttctcca gctccatgca agaaaagaac
    26161 aagcggataa gaagcggact tgttgtaatt ttcggggtat aaaatgcgat aaggcaaggt
    26221 atctcctttg taaataaata attttttcag aaacaactcg ttttgagcct gagacaatat
    26281 actcacgaac aaaaaaaggc tcaacaaaga aaaaaacttt ttcataaact gatgattttt
    26341 acaaactatt gtttttcaat gtatcatatt attttcggtt ttggtttaag gaagttttag
    26401 ttgtagctgt tggataaaca taaataatga ttaaacaaaa atactttcta aaagttctat
    26461 tttttccacg atcgataagg attttttttg agcatggcgt tgtaatattc aggttttcct
    26521 gtaacttctt ctcccaacca atcgggcttt aaaacctctt ggctttcatt ttctaattca
    26581 atttcggcca aaatcaatcc ttcattttcg ccaaaaaatt cgtccacttc aaaacgctgt
    26641 ccccgataaa caacttcgta acgctttttt tcgataatgc cgggttcaca aattttcatt
    26701 aattcctgcg cctcgtcaac agggatttct ttttcccact cgaagcggct caatcccgaa
    26761 agattacttt tccccttaat agtaaggaaa gcagaaatat ccataatacg aactctgacc
    26821 gtacgctcag gatcagaaca caggtaaccc tgaataatgc gataactttt ggaagcttga
    26881 gatttaaatg ctcccgttac caaaaatttt ctttctgttt caattttcat ttttaaaaga
    26941 tatttgtcga tttaaaatat ctgtagcaca tttttccacc aaattctgat tttgaaattt
    27001 tttcatgctc aacttcgaag aaaaatgttt tctctactca tttttgatac tcgacttctc
    27061 aggtttgaaa caacttttgc aattttccct gtcgaaaaag aaaattttta acgattgcaa
    27121 atttccataa aagaacaatt gtcgcagttt tctttaattt gcgtttgctt aaaaggaatt
    27181 tcaggattaa aaatttcatt cagcaataaa gtcagttttt ccttaaattc atgctcgaaa
    27241 atgccgaaat cttccacctg aatagtgtgc ttctccggtt tgaattgaat tgaagaagaa
    27301 aaaccgctgc taaaaatttc cttcaaatag taaatggcgg gtgaaataat tttcttttcg
    27361 gctttagaac gataaagcag acaatacata aaaacctgaa aaatatgttt tggccgattt
    27421 ttcatcggga gagaaccatc aaacaaatca tccaacaatt taaaggttgt atttcccgtt
    27481 cccgttttat aatcaatgat acgcaaaaaa ttttccgttt catcaatccg atcgatatag
    27541 ccttttatat tcactcgcaa gtttagcgtt tcgagcggga agcgaatact gcaaaattcc
    27601 tcagcgccga gataacgaaa aggtgtcaat tgctgatcga catacaataa acgcatcaga
    27661 aactttttca gtattttgct gatgagtaaa ttattgcctt ccaatggcgg gatttttccg
    27721 ttttgttcgt ttttaaaaaa atgttttgaa aaagccagtg aaatggcctc gtccaccaac
    27781 gaaggctgac gcaaaatttt ctcaaaatct tcttttccca gctgcacatc tttcaatggt
    27841 ttgtaaagca attcagcaga agagtggaac aaagtaccga acaaatcatt ttccactgtt
    27901 tcagtaattt cttccgtttc tttgattcgg gctatttgag cataataaaa tttcagcgga
    27961 caatctatgt aagcattgat ggagctggca gaaaggtaca acttgtcatc gccggaagaa
    28021 acaaaacgct gcaatttttc gcggataagt gagtcctttt ctaccgaagc ggagactgca
    28081 tctttcaaaa aaatatcgta agtaatgttt ttacgttcaa cctttgtgtc gtaaagatat
    28141 tcgagctgat acagaaatct gctgacttct cccgtctgaa aaccttcagt actggaatta
    28201 tataaaaaat aaaccttttg ggcatgatga atcagcctat aaaaattgta ggcactaatg
    28261 gcatcctgat gttcctttgc aggcaagccg aaaccacgac gcaaattgta gggaataaaa
    28321 ctgtgggtgg gagcattttt tggaaaaaca ccttcattga aagaagtcag caaaatatta
    28381 tcaaaatcca aacccctggt ttccaacacg cccataattt gcaaaccttc cagtggttca
    28441 ccgacaaaag gtaaagtaat aaaggcagaa tattgaaaaa tcaatcgcat caaagtatcc
    28501 atcttcattt cgattccttc tgactgatat tgcaaaatat cggacatgcg gtttagaaaa
    28561 cgataatatt gaatcacaaa atcggtttcc attcgatatt gatgatttcc aaaaccaatt
    28621 tgctgaaaat ttttccgtaa aatacgtaaa atgttcaaca aatacgtcag catttcgcgt
    28681 gcatcatttt gcggccgaaa aatagcagta aacaactcat tgatttgaag ttgttcaggc
    28741 cgaatgtata tcgagttgtt ttctaccatt tgctgaagta tatttttgct gtcgtctcca
    28801 caaagcaacg taatgtagga atgctgcaaa atttcggtta ccggttgatg ataaaacaac
    28861 acttcttcat cggaattgcg aattcgtttc tgcaagtcga aaacatgctc aatcaacccg
    28921 gaaacagggg tgcttttgag cggaaaaccc atagtaacat ttaccttttc gatgtgctcg
    28981 ggcagggagt aaagcgcagg caagagcaaa ttttcatttg gcaaaacaac agccgtttta
    29041 atccaacttt cttgctgaac ggatgcatcc tgaggataaa tgcgatttag aatttcataa
    29101 atctgtttga tctgaccaat ttcagaaggc acggctatca gctcaaaata ctgttttttc
    29161 ctttcttcga caggagtttc aatggaaaat ttggaaggaa aagttttggt attttccatg
    29221 aaaaaagttg aggccgggtt atcggcatcg cgtaaagccg gagcttcata atcccagtaa
    29281 aaatcggctt tctctttttt ttggaggact tcaaaaagag ttttttcgca gcgattcagc
    29341 gcattgaatc cgataaaaac aaattgtttt ccttcccatt cttgaatttt ttcttcgctt
    29401 tcgagtcgtt cggctaggtt gcgataaatc ataccttccg ttgccatgtt ttcccgcttc
    29461 aattcctccg taaaatcttg ataaacagga taaagaattt tccaaatttg tataaaatct
    29521 tcctgcgttt tttccttagg aatcgggata aagtttttcc aaaactgacg aatagcatcc
    29581 aactgatttt cggaaagata attgaacatg cggtcaatct gtttcagttc agtaacattg
    29641 gtaaacagtt ggcgggcatc aatgcgatat ttgtcgatat cgtcgaagtc gttgagcaac
    29701 atttcgcccc aataagcaaa agaatcgaaa ttttcgttag taccactttt ttccttataa
    29761 attctataca gacgaaacag gagactcaac ctgtcagcca aatgaaattg ggctacagag
    29821 gtaaaacaat cgttaatggt caatatttcg ggcgagaaaa gcggtttttt ggctatttgc
    29881 gaaatgtagc gctgaaaaaa catgcctgcg cgcctgttgg gaaagacaaa ggtaaaatcg
    29941 gaaattttat cttcatagtg gttgtaatat gtttgagcaa tacgatagag aaaagattcc
    30001 attgttgaat aataaaataa aacctgcttt ggttgaattt accaaaatta caacaaaagc
    30061 agctgagatt aaaattttaa tttcatcgaa aaattacttt ttccagaacg ctggtgtaaa
    30121 aagcagcaaa accgtgaaaa gttcaagacg tcccaacaac atcataaacg acaggaacca
    30181 tttggcaaaa actgggaaat aagaataatt tcccacaggc ccaaccattc ccaagcctgg
    30241 cccaacgccg cccaaacaag ccacaagtcc gccaaccgat tcaataaaat taacacccga
    30301 catgcaaaga attaagattc ccagaatggc tgcaatcaaa taaagcaaaa caaaaaccag
    30361 cactctggta accacatcct gaaatacaat ttttccgctg taacgcaccg gaacgactgc
    30421 ctttgggtgg ataagtcttt tgaattcgta ataacaaaat ttgaaaacaa tcacgatacg
    30481 caccatttta atagccccac tggtagaacc tgccgaagca cccgtcagca tgacaatcag
    30541 caaaattacc cacacaaagg gtttccattg catataatca acggtagtaa aacctgtggt
    30601 tgacataagt gaaccaactg tgaataaggc atttctccat gattcttcca ctttgtgaaa
    30661 agatatgggt cgagaaaagt caatcatgga aagtgcaaca ataatagcaa agccaaacaa
    30721 tacaatcaga taataacgca actcctcatt ttggcgtatt ttttccagct tatttttaaa
    30781 tccgaaataa taaaggctga aattgacacc ggataaaatc ataaaaataa ttatggtgta
    30841 ttgaatgtaa ggcgaatccc aatattggat actgttttgt tttgttgaaa atccgcctgt
    30901 agctatggtg gcaaacgaat ggcaaacggc atcaaaccaa gtcattcctc ccaatcgcaa
    30961 caaaatagtt tcggaaaaag tgagcaaaaa ataaattaca aacaatcttt tagctgtttc
    31021 actgattttg ggatgaattt tatctttcgt agggccactg ctttcggctg caaaaagttg
    31081 tgccccgctt gtgccaaaca taggcagcaa agccaaagaa ataacgatga tgcccaaacc
    31141 acccatccag tgggtaagac ttctccaaaa cagcatggag tgggacagtt cttcaatatt
    31201 atttaaaatg gaagcgccgg ttgttgtaaa tcccgaaatt gtttcaaaaa aggcatctgt
    31261 aaaaagagga atgcttttac taagccaaaa gggaagagat ccaaacaatg aaaaaattat
    31321 ccatgccgaa gttaccacca gcgaaccttc gcgtttacca acagacatag gagcattttt
    31381 tccaaaaaag aaacccgcaa agccgacaac ataacaaagc aaagaggtga aaataaaata
    31441 aacaccgtca ttttccctgt aaatcaatga aataaaaaac accaacaata aagcagttcc
    31501 ttcactcagt agaagactgc ccaacatacg aatcaccagt ttaaaattta tttcacgatt
    31561 catggtttta gtgaataaaa tggggtcagg tgttgaaaaa gggttcgagt ttacgaatag
    31621 ctgaagaaat acaaaaaact atcacatgat cttcgggttc tatacttgtc tctccactga
    31681 ctatcattcc ttttccattt ctcacaatac caccgatatt tacattttcg ggcagtttta
    31741 aattctttat tttagaacgt gtaatttttg aacctgcttt ggcaataaat tccacaactt
    31801 cagcatcggc agaagttaaa tttctgacat tcaagacaga agcatttaaa gtgatttgat
    31861 aaatataact ggcagctatc atttttttat tgataaccgt gcctatatcc atgctttctg
    31921 caagtgggat ataatcaatg ttttcaactt ctgcaatcgt tttttttaca ccaaaacgtt
    31981 tggcaaccat acatgccaac aaattcattt cagaatttcc tgttgctgcc acaaaagcat
    32041 ccatttcctg aattccttct tctctaagga catcaatatt tcgaccatcg caattaataa
    32101 tcagagtatc cgggaacttt tcagccaact ccatactcct ttcattattt atttccaaaa
    32161 ctttggtatg aatatgatcc ggaagtcttt ttaatgtttt ttgtgcaatt cggctgccac
    32221 ccaaaaacat aacgttccta ataggaaaat cttccttgcc tgcttgttcc cttacgaaat
    32281 ttaaattttg aggtgtagtg ataaaataca caatatcatt gtccaaaatt tcgtcgctgc
    32341 ctttgggtat aatggtctgc atcccacgtt ttatggcgac tactctgtac ttgtcatgat
    32401 caaaataacc ggtactgaat tttttgttga taatttccga attgcttcga accttaatac
    32461 ccagcaaaat cagagcatca ttgcaaaaac tcatattttg tcgtagccaa ctggttttca
    32521 atgattccga aatttctttt gcggcaagca cttcaggata aatcaaataa tcgaccccca
    32581 atttcttgaa aaaatccttg ttttcgggga aaagatattc gtaattatca atacgcgcaa
    32641 gcgttttttc ggcacccaaa ttggtggcca acatgcaggc agtcatgttt acactttcat
    32701 agggagtaac agctataaac aaattggcat tttctacgcc aatttccctc aagtgattga
    32761 tggaagtgga agaacccaca ctggtgagta aatcgtagtt gctttccaaa tccatcagcc
    32821 ggtcaggatt ttcatcactt agggttattt ccatactttc ccgagccagc aattttgcta
    32881 agtgcgttcc gacttctcct gccccaacaa taataatttt cattctttga aatcttttct
    32941 gaaaattctg ttgattatag aatttttatt ttttccaaaa ttattggaag aaactatctg
    33001 ttgttttttt gaattatcgt tttcagtgtt tttttgtagc atgtcgataa tggtttttga
    33061 tatcccattt tcgtccatac ccagcatggc atataattcg tcaggcgaac cttgttcaat
    33121 aaaattgtca ggtatgccaa gtcttttcac ccgaaccgaa taattatgat cggcaaaaaa
    33181 ttccagcact gcactgccaa gacccccctg tataacgcca tcctcaatag tcaaaacgta
    33241 tgaaaattta gctccgattt tatggagcat attttcatcc aaaggtttaa gaaaacgcat
    33301 gtcataatgt gcaattgaca aatggcactc gttttcaact ttctcaatag ctttttcggc
    33361 tttcactgcc attgttccaa tagttaaaac agccaaattt tcaccctctt tcaaacattc
    33421 acctttacct acttcaagcc tctgcattgg ttttctccaa tcgacaacag atccggctcc
    33481 acgaggatag cgaattacaa acggtcccaa gtcgggttgt tgcgccgtaa acatcaaatt
    33541 gcgcaattca atctcatttc gaggagcggc aatggtcata ttaggaatac aacgcatata
    33601 ggccaaatcg aatacaccgt gatgagtggg gccatctgca cccaccaatc ccgctcgatc
    33661 gagacaaatc actacatgca aattttgtaa tgctacatcg tgaatcacat tgtcataagc
    33721 tcgttggcaa aaagtcgaat aaatgttaca gaaaggaatc attccttctt tcgccaatcc
    33781 ggccgaaaaa gttacggcat ggccttcggc tatacctaca tcaaatactc tttccggaaa
    33841 ttccttttgc atgatgttca tggaacaacc actgagcatg gcaggtgaaa cccctacaat
    33901 tctatcgttg cttttagcca attccagcag ggtttctccg aaaacatgct gaaaaagagg
    33961 aggctcggaa acagaattgg aagaagtttt ttctcctgtt tccacacaga attttcccgg
    34021 tgaatgccat tcggttacag ctttttcggc aggctcgtaa ccttttcctt ttttggttag
    34081 acaatgcaaa attttagggc ccttataatc cttaatttct gagagaattc tcaccaattc
    34141 attaacatcg tgcccatcca ccggtccgaa ataccgaata ttcaatcctt caaaaagatt
    34201 gtgctgtcgg gtaagggtag ctttcagcgc attgttgaaa cgaatgatat tgtcttttct
    34261 cgactcatca atcaaattca accctttcag cattttataa attttgtacc ttattttgtt
    34321 gtaggtctga gaagtagtaa tatcaatcaa gtattcggta ataccacctt tcagggggtc
    34381 aatagccatc tgattatcgt taacaattat caacaagtca ttgatatttt tactcattga
    34441 cacattgttc aacccttcaa aagcaagtcc gccggtaaga gctccatcac caatgacggc
    34501 tacgacatgc ctgtcttttt catttttcag tttggaagca atatccattc ccaatgatgc
    34561 tgaaatggaa gttgaagcat gaccaactcc aaaagcatca tattcacttt ctttgggaga
    34621 agggaagcca ctaattcctt taaattcacg attggtgtga aatttatcac gacgaccggt
    34681 aagaattttg tgcgcatacg cctgatgacc cacatcccaa acaatgcggt cgtagggagt
    34741 attaaataca taatgtagag caaccgtgag ttccacggca ccaagactgg atgccaaatg
    34801 acccggatgt tgggaaactt cgtcgataat aagtgttctt aattctcggc aaaccgcttc
    34861 aagttcttct tttttaagaa ctttcaaatc cttagatgat tcaatgtgag atatataagt
    34921 aaaattttta ttttccattt tctgagatta tgattgtgca caaaattaat acatttttta
    34981 atgtttgcta ctttttaaac aaataattta ttgctttgta tttataccgc ctttataaaa
    35041 aagacaagtt tatacacaga ataattgaaa gctataaaat tattttttaa ttatttccga
    35101 ctgccttata taacaaattg aaaaaaaata tgattttttt ttgataaagt aattttttca
    35161 tctatctttg caccgaaatt caattttgaa gaaatgtatc ggaaaaataa ttttttagga
    35221 atcgtcgtgc tacttttggt ccacaatcaa aggtgaggat ggttcttatt attttttata
    35281 taataaagca taacagataa aacctttctc accttcatag agaaaggttt ttctgttttt
    35341 attgaattta cttttttact tagaaaaagt aaaaagcaaa caaataattg atttaaaatc
    35401 agtaagtaat ataaattttt aaaataattt ttgaatggga acttcaataa acagtgttga
    35461 agaacaatca atgataagca ggttgagaag tacaaccagc acatcaggaa gaagaatggc
    35521 cggagcgaga gccttgtggc gagccgccgg catgaaaaac gaacaaatgg gaaaacccgt
    35581 gattgcgata gtcaattctt ttactcagtt tgttccggga cacgttcact tacacgaaat
    35641 tggtcagaaa ataaaagctg aaatagaaaa actcggctgt tttgcagcag aatttgatac
    35701 catagccatt gatgatggaa tagccatggg gcatgacgga atgctttact cgctaccttc
    35761 ccgcgacttg attgccgata gcgtggaata catgatgaat gctcacaagg ctgatgccat
    35821 gatatgtatc agcaattgcg ataaaattac tcccggtatg ttaatggccg ccatgcgatt
    35881 gaatatccct actgtttttg tatcgggtgg gccaatggaa gcaggcgaat tggatggtgt
    35941 acagctcgat ttgattgatg caatggtgca gtcggctgac gactcgattc ccgacgaaca
    36001 aattcaaaga attgaaaatg cagcctgtcc tacttgtggt tcttgttcag gaatgtttac
    36061 cgccaattcg atgaactgtc tgaccgaagc tattggactg gcacttccgg gaaacggaac
    36121 aatagtagca acacatgtca accgggaaaa attgtttgta caagctgcca aactgatcgt
    36181 tgaaaatgct tataaatact atcgggatgg ggatgcttct gttttaccac gttccattgc
    36241 tacacgtgaa gcttttctta acgccatgaa actggatatt gccatgggag gctctaccaa
    36301 taccgtttta cacttgctgg ctgttgctca cgaaggcgaa gtaaatttta caatggacga
    36361 tattgatgaa ctttcccgcc atacaccttg tttatgtaag gtggcaccaa acactcaaaa
    36421 ataccacatt caggatgtca atcgtgcagg tggaattttg ggaatattgg cagaactcag
    36481 tcgtggagga ctcatcgaca ccaatgttcg tcgcgttgat ggaatgactt taaaggaagc
    36541 cattcataaa tatgatatta catcgcccca tatttcggct gaagttattc agctctacaa
    36601 aagtgcaccg gccaaccggt ttaatttggt aatgggttct caaaattctt attataagga
    36661 attggacacc gacagaacaa acggatgtat tcgtgatttg gatcatgctt atacaaaaga
    36721 tggcggactt gcaattttga aaggaaattt ggctcaggat ggatgtgtta ttaaaacggc
    36781 cggcgtagat gaaagtatct ggaaattctc cggtccggca aaagtatttt cttctcagga
    36841 tgcagcttgc gaaggaattt taagtggaaa agttgtggca ggtgatgtgg tagtcatcaa
    36901 ttttgaagga ccaaaaggag ggccgggaat gcaggaaatg ctctatccga cttcctatat
    36961 caaatcgcgc cacttgggaa aagaatgtgc attgattacc gacggaaggt tttctggcgg
    37021 aacttcgggg ctttccatag gtcatatttc gcctgaagcc gcagcaggag gcaatatagc
    37081 attattgcgc gatggcgaca tgattgaaat cgatattccc gcacgttcca tacaagtgaa
    37141 actcagtgaa gaagaattgg aaaaacgccg ccaggaagag ttgtcgagag gaaaagaagc
    37201 ttttacgccg aaagaccgtc agcgttcggt ttcaaaagct ctaaaagcct atgcttcaat
    37261 ggtaagttcg gccgataaag gcgcagtgcg gttgatttaa aataaaaaat gattttttac
    37321 agtacaaaaa gtttattatt agattcaaag attcccaaat gaaaaaagga ggaataataa
    37381 taatatgaaa atgacaggag caaaagcatt gatagaatca ttgctgcaag aaggagtaga
    37441 aaccatattc ggatacccgg gcggtgccat tttgcccgtt tttgatgctt tatacgatta
    37501 cgaacggaaa gtaaatcata ttcttacgcg ccacgaacag ggtgccgctc atgcagctga
    37561 aggatatgcc cgcgtttcgg ggaaagtggg agtttgcctg ataacttcgg gaccggcagc
    37621 aactaatgct attaccggaa tcagcgacgc catgctcgac agtgtcccat tgttagtcat
    37681 tacaggacaa gttggagcaa gtttgcttgg tacggatgca tttcaggaaa ttgatgtgat
    37741 tggtattaca caacctataa caaaatggtc atatcagatt cggcgggccg aagatgtggc
    37801 atgggcagtt gctcgcgcct tttacattgc atccaccgga cgtcccggac ctgtagtact
    37861 cgattttacc aaaaatgcac agtcggaaga agtagatttt caatacaaac cttgtaaatt
    37921 tatccgcagc tatgttccgg ttccagaagt taatttttcc caaatcgaaa aagcagccga
    37981 actgattaat gccgctgaaa aaccttttgc tgtggttggt catggcgtga ttctcggtca
    38041 ggcagaaaaa gacttgatgg aatttttgga aaaagctgat attcctgctg cgtggacttt
    38101 actgggggct tcggccatgc catcggactt tcggctgaac aaaggatttg taggcatgca
    38161 cggaaatgta gctcccaata tgaaaaccaa tgaatgtgat gtattaattg ccatcggtat
    38221 gcgcttcgac gaccgtgtta caggcgatct gaaaacgtat gccagtcaag ccaaaataat
    38281 tcatattgat attgacgcag ccgaactgaa taaaaatctg aaagtggaag tgccagtttt
    38341 gggctcggta aaagacgtgc taccggcttt aacaaaactt gttaaacctg ccaaacatac
    38401 cgaatggatt gattcctttg atatttttga aaaaacggaa tacgaaaaag tgattgaacg
    38461 cgaggtattc cccaaaagcg gaaaaattac aatgggagaa gttatccaca aagtatccga
    38521 agccactcat cacaatgcta tttgtgttac tgatgtgggg caaaatcaaa tggcatcggc
    38581 tcgctatttt aaattttcac aatccagaag tttgataacc tcaggcggac tgggcacaat
    38641 gggttttgga cttccggcag ctatcggcgc aaaaattgcc gctccggaca gaacggtttg
    38701 tctgtttgcc ggcgatggcg gtttccaaat gactattcag gaacttggaa ccattatgga
    38761 aaacaatatc ggcgtaaaaa tgattgtgct cgacaataag tttttgggca tggtgcgtca
    38821 gtggcaggaa ttattttatg aagaacgcta ctctttcacc aaaatgaaaa atcccgattt
    38881 tgtagctatt ggaaaggctt ataatattga tggaaaattg gttaacgaac gggaagaact
    38941 cgatgaggcc attgccgata tgctgaagga tgacagacct tatctgttgg ttgtaaatgt
    39001 agaaacaaaa ggaatggtat acccgatgat acctgccgga acaagtgtaa ccaacattat
    39061 tctggagtag atttattact gaaatttcaa aaatataact tcagagattt atcagcattt
    39121 tttaaaactt tgcattatgg aaaagacatt atataccgtt atcatttttt cggaaaatac
    39181 ggttggcgta ctgaaccaaa ttaccgtcat cttcacgcgt agaggtctga atattgagac
    39241 tttgtcggtt tctccatcgg ccattgcagg tattcataaa tttacgatta ccgtcattac
    39301 cacacaggat gttatcgaaa aagtagtgca gcaaatcgac aaacgcgtcg atattctgaa
    39361 ggcttactac aataccgatg aagatcttgt acatcaggaa attgcattgt acaaagtggc
    39421 aacggacaaa ttgctcgaat tggggtctat cgaaaaaatg attcgagatt accaaattca
    39481 tgtgttggaa ctcaccgaaa actgtgtggt ttttgagaaa accggacatt atcaggaaac
    39541 acagaagctt tttgaagagt tgagttcgac tattggcgtt ttgcaattta ttcgttcggg
    39601 acgaattgcc atcaccaagt cgaaagtgga acggctgagc gatatgctcg aaaaaatgag
    39661 taaacttgaa caaaaagtca atgccacaac cgaaacacaa gaataattgc aacgacaaat
    39721 tgttgcaata ccaacttaaa aatacgagtg gaaaaagagt tttcatatat ttttaaaatt
    39781 gctccgtcca atgtcgattt tcagggaaag ctcactttag cctcgctaat agagttttta
    39841 atggaagcag caggcagaca tgccgatgga aatggttttg ggatgcggca attgaatgaa
    39901 ttcaatgctt cgtgggtggt gctacgaatt gcattggaaa taaattatta tcctgaacaa
    39961 tatgacgaaa taaaaatatc gacatggatt gaaaaaattc agccgataaa aaccatacgc
    40021 aatttttgta ttgaaaatgc acagggagaa attattgcct cagccacttc agaatggatt
    40081 atgatggatt tgacccaacg caaaccaaaa gatttgcttt ctctggaagg ttttgaagaa
    40141 tttgccacag ggaaagaaat cccggccggt aaattggaga aacttcctcc cgtgaaaggt
    40201 gagctggtag acaaaataac cgtgaaatat ggcgatatcg atattaacgg acatgtaaac
    40261 agtatccgtt acgtacaatg gctatgcaat tgcttcaatt tgtccgacta tcgggaaaag
    40321 caaattgaac gcttcgagat tcactatatt tccgaattac atgaaaacga aaatattaag
    40381 atttttcgag aagaaaagaa gaaaaacgat ttttatttcg aaatagacag tgataaaaaa
    40441 acgacttgca aagcaagaat aagcttttcg gaaaagtaac aaataaaatt tttagcaaac
    40501 aattgataac aaacaactta ataacaaata atttttaaaa actacaaaaa aatggcagta
    40561 attaattttg gcggtgttga cgaaaatgta ataacccgcg aagaatttcc tttgagcaaa
    40621 gctcgtgaag tactgaagga tgaagtaatt gccgtaattg gttacggtgt tcaaggtccg
    40681 ggtcaatcgc tcaatttgcg ggacaatggt tttaatgtca ttgttggaca acgcaagggc
    40741 ggtaaaactt gggaaaaagc tgttgccgat ggctgggttc ccggcgaaac acttttcgat
    40801 attgaagaag ctgcagaaag aggaaccatt attcagtatt tgctttcgga tgcagcccaa
    40861 atagaagttt ggccacgtat aaaaccgtat ctgacacccg gcaaggcttt gtatttttct
    40921 catggatttg gcatcacctt taaggatcgt acgggcatta ttcctcctcc cgatattgac
    40981 gtaattatgg tagctcccaa aggttcggga acttctttac gtcgattgtt tctcgaagga
    41041 cgtggattga attcttccta cgccattttt caggatgcta cagggaaagc ctttgacaga
    41101 gtgattgcac tcggaatagg tgtaggttca ggctatttat ttgaaactac ttttgaaaga
    41161 gaagtttatt ccgatcttac cggtgaacgc ggaactttga tgggagctat tcagggactt
    41221 ttgtcggctc aatatgaagt gctcagagca cacggacatt ctccttcgga agctttcaat
    41281 gaaactgtgg aagaactgac gcaatcgctg atgccacttt ttgccgaaaa cggtatggat
    41341 tggatgtacg ccaactgttc cactaccgct cagcgcggtg cattggactg gatgggccca
    41401 ttccacgatg ccaccaaacc ggtattcgaa aaactttata acgaagtggc ttcaggtcgt
    41461 gaagcacaac gttccattga ttccaattcc cgccccgatt acagggaacg gcttgaagag
    41521 gaacttaagg ctttgcgcga aagcgaaatg tggagaacag gcgctgttgt tagaaaactt
    41581 cgtcccgaaa acaattaaat ttttttgatt aagaatttca ctagaaacct gtctgttttg
    41641 aaattaattc aaacgacagg ttttttatga aaagcccgct tagctttatt atattatttt
    41701 catgttgttt atatgacaat catggcagta tttcaccaac tgtgtaaatt aaaaagttgg
    41761 agcaaaaatt aaaatcttat ttttcgccga aggaagaata aattgattaa gaaaaatgtc
    41821 ctaattttga ctagtaagtc aaattggttg ttaaaaaaat tgtaagttac atattatcat
    41881 atctttcctc tataaatctt aggttaatat tttgaataag ataatcgtat tgttttgata
    41941 atcagattat cggtgatatt taattctgta aaaaagacaa agctaaagcc tgcgtctttc
    42001 ttttttgaaa ggcggaaaca tgaaaaataa tcagtcgagc cttgaaaagt ccattttttt
    42061 attaaattat tattgacttg gtaaaacata gcgtttatca agtcaacaat tttattttaa
    42121 attcgagcca atttctattt attcatcatc ctcttgaagt taaatgcagc tgctgcaagc
    42181 attacatttt atcgcctgta attcctttgt agaaattatg atttaggcgg tggtctgttt
    42241 ttaaataacc gttaataatt tttacgaaaa ctttgaaaaa attatcgaat ttttaattcg
    42301 atttttctac tcgatagctc taatggacaa tttgggatta tgagatgaga tgaaatgaga
    42361 ggaaaagtat tagaattcaa gtttaattcc agcacttaat gtagcaactc catagttaag
    42421 cccaatattt cctagcgata tgtttgcttc agcgcctgca ttttgtaatg acaactcggg
    42481 tgaaccattc gaaaattttg ataatgttat taagttttga caattaattt gagctattat
    42541 ttttccaagt ccgaattttt tggtgttttt tgagtcgaaa gtatagttca tactcaaatt
    42601 gcgcaatcgc aaatacgatg cattcatcaa ataacgcgtg ctattacggt tcgccatttg
    42661 agagttataa agaagtggtg tgtttgagtt tttattttca ggtgtccaat aattttgtag
    42721 cgaaaccatc ttattgttta tgtcataggt gtttacgtat gaattttgtc gcataccgtt
    42781 gtgatatatc caatttccga gttgaaatga aaagtgtatg ctgagttcga attttttgta
    42841 tgcaaagaaa ttactcactc ctccgtataa tttgggatag ggtgtcttgt ctgttatcaa
    42901 tacagactga ttgcgatact caacagatgt aagcatttcg gcatccaata atttacctgt
    42961 tttagtttta tttgtttcgg gtcctataac ttcatatatc atttcatgtc cagtttctgg
    43021 gttgacacct gcccataatg gtaggtagta taaacctagt gggtaaccca cataagctct
    43081 atttattcca ttttcaatat agttttgctg agtcgaaagt tttgaaatct ttgtattaaa
    43141 cattgaaatg ttaaattcgg ttttccattt gaataaacca tcataatttg tagtttttaa
    43201 atctaaatcg aaaccattgt tattcaaatt acccgaattt attggaactt ctgtgttaaa
    43261 gtatccttgg aattttccaa tattgtaatt caaaagtaaa tcagagttac gttttaaaaa
    43321 ccaatctagg cttgctgata ttttgttttt taaagctgaa atattgaatc ctacattcaa
    43381 ttgtagttgt ttttcccaac gcacatccaa gtttgctatt ctgtccaaca caaagccagg
    43441 gctacctgcg tattcagccc aattgagtac tcgcgattcg gcaggaaaat taccaacctc
    43501 tgcatttcct acaaaaccat aacttgttct aaattttaaa taatcaattg attttatctg
    43561 tgaaaaaaag ttttcttcac ttaacatcca actcattcca agccccggga aaaaccccca
    43621 tctattatca ttaatgaatc gagatgtgcc gtcggctcgt gcgtttaatt caaaaaaata
    43681 cttctcgtct aatgcatatt gagttctgtt aaaaaaggat acaaatcgat agccatccat
    43741 tcctgccgat tgtcgtgtta gtactttttc ggtgtttgca ttagttagct gaaagtaatt
    43801 ggttggaaaa ccctcaaagc gtgcaacatt aaaatcgctg ttaaaggatt gaatgttatt
    43861 acccagcata acttttatga catgccggtt gtttattttt gtattgtaat tcagtaaatt
    43921 gtttacatta aatccatagc ttaaggttct tgaatatagt attataccgc tttttgatgg
    43981 gtcgatagct tcatcttcgc gataaaagtc ttttgactga aaatatcgat acctgctagt
    44041 accgggtgtc atactaagtt cagatctaaa tgcaaatttt tgcatcaatt taatatcgaa
    44101 ataaccaagt aataaaccat taaaattaat attgtcatcc caagtttcac tcattctata
    44161 aagtggatta ctgccttttt tgtcatttat attgcgtgcg tcgaaatatt tacccgaacc
    44221 atctggcgca tatattggga gcaatgaaga acgtgaaaat aaagactcca acttgtcaaa
    44281 agttgaattt ttcgaagtat ttagagtgaa atttatgtta aatccagtgt taattctctc
    44341 cgatatctta aaatcatttt ttaatactaa agtgcttctt ttcttgtttt tattatcaac
    44401 tagttcattg tccgatttat gcgataaact aatgtagtaa ctatttgact tttgtccacc
    44461 ttgaaacgat aagcggacat cttgagaaat tgcagcctgt gttaatgccg attcccagtc
    44521 ggttccaaga gggttgttaa gcaaataagt attcaaccaa tcgcggttaa aacctaaaaa
    44581 attagcataa tccggtgaaa aagggaaata tttgatagga ttgttatctt ctaatgtttt
    44641 attttcagga tacctgttat agtaagctgc atcaagtaat gaaattagtt ctttgccctt
    44701 aatgaatttt ggtttgttcg ttacgtaatt gattgatgtt ctatattcgg caattgtttt
    44761 ttgtttcgaa ttcaaatcac ctgattttgt ggttataaca ataactccat tggaagcacg
    44821 tgagccaaaa atagccgctg aggctgcatc ttttagaaac tctactgaag caatatcgtt
    44881 agggttaatg tcatttaagc caatggtttg aggaatcccg tctataacaa caagtgggtc
    44941 ggctgacgag tttattgacg aaacacctct tatgcgataa ttttgattcg atgcccaaag
    45001 tcctgcaaca ttaccttcga aactattccc aatatttccc cctacattgt tttgaattgc
    45061 aaatttaggt aatgattgta tagctcctgt agtttctcgt ttttgcatgc tgccataccc
    45121 tataactaat aactcgctga gcaataagtt gttggagcgc atcgtcactt tgtaaaagtt
    45181 attgttattc aaaagtttta gtgtttctcg cttgaaacca tgcataaaaa aatataagct
    45241 tgaatctttg ggtatgctat aaatattaaa actgccatct tcgttactta tacctagaat
    45301 gttgccttgt ttgtcggtga tgtaaacgcc aggtaattgt tgttgtaatt catcaacaat
    45361 tacgcccata atacttttag actcttttag agtcaaattg ttagtatctt tcttggcacg
    45421 caaaaacaca taaacatctt ttatcgtata agtaatgtcg gttcctgcaa acaatgtatt
    45481 taaagtctca tacaaatcaa gattatttgt ctgtaaactt ttaatgccaa attcattcag
    45541 gtccgatgtg ttgtaaacaa atactacatc ccatttaagc tcaatttcgc gcattatttg
    45601 cttgaaatca gcattttcga aatttaacga tattttttca tcatgttttt gtgcccacgc
    45661 tttagcgctc gcaagattta aacaaatgat aattacgatg agtaataagc gattgaatat
    45721 gttttgtttt cttctcaatt cgatttttga cataataggc tccgtattat agcttgccgt
    45781 gcattatact tattcggatt atatacattt tattattctt tatcttttta gtgcaggatt
    45841 gtaaatgtat tcattctcag gtattaagca gtacagtctt tcgtcgttcc aatgtaatat
    45901 actgtcagtt attaacaaag ctctatttcc taaaggtata ttacccaatt tgtatggcat
    45961 gggtaagtaa ctagccgcat tactatcgct caaagatgct aagcgaatat tatcactcct
    46021 tctaatacct tcgaaatgca tttctctgaa tctttcatcg atgattgctt gaaataattg
    46081 agtttttcct gcgaaagtat aattgtcaag tcctgcacgt tttctaacta tgttaagttc
    46141 gttaattgaa gaactaagat tatccttgtg gagataaatt tctgctcgca taagatgaaa
    46201 ctctgctgca cgaagccata ccacatttga agttgggtat gcatgtttta gactagtcca
    46261 ctgccctgca ggcgattctt ttcctatgtt tttcgtaata tatatcatgt ttttaaatct
    46321 aacatcgttt aaagtatcgc ctttcaaaaa acttttaatt aatgcactgt ccataacaat
    46381 tctgaaacgg agatttgtta agtcgctatt ttgaagattt tgaaacgcac tccaatgaaa
    46441 catgctccac tcttgtgtag atgtaggacg gaatgcagag ttcccatgaa aacccataat
    46501 aatttcactt ttactattag cttgataatt tgttttatct acaattcgaa aaagttgatt
    46561 atagttattc gtagtttgaa gcgggtattt attcgaagct cccgtttgat ttccaagcaa
    46621 ttggttgata tataccaaag ccgaatcaaa ttggttttgt tgaaaatatg ctttagctaa
    46681 cattgctatc gctacgtctc gcttacaccg atattgatat gctacgggat gtattgtttt
    46741 ttcgtaatat tgtggcagta gtttgcgtgc attttgtaga tcttctataa tatactgatg
    46801 cacctgttca accgtttttc gaggttcggg tatatcttcg aagcctaaaa tggctctttt
    46861 tctatataag cttgctggaa gttgaagtgt gcttgggtgg tattgacgcc caacatacaa
    46921 gttgttgtaa tactcaacac aggcacgcaa taaatatgcc tctcccaaaa gtctttcggc
    46981 attgagcgtg tcattaaaat tacgtaaatt tggtaattta ttttcaattg acttgatcac
    47041 tacattactg ttgttggtga caaatgatcc ccacattaaa actgatccag cctgataatt
    47101 atcagtgtta cactggtagc tgaagtaata attgtacact gagatggata gaaaatcagg
    47161 taatatgctt gggtttttgg tgttcgtaat ctcgtccgat cctagtgttt gagttaaaac
    47221 tacattgttg taataagagt ttgagcaaaa agctccataa gcaccgttta tacaaacatc
    47281 aatgtcattt atattgtata acaacgattc tatgctttgg ctattctcat tgcgtataga
    47341 cgacaagaaa tcctcgcacg aactcataat tattgaaatt ataagtaatg cgaaagtttg
    47401 taaaatattg aattgtgatt ttttcattgc tataaattga tttatcacga aactttttca
    47461 agcctctaag atgcaaatat acaaaaaaag tctaatccct aagtttttgt ttgtttttta
    47521 acattgatat tgtggctttt acaatgtatt gatttgaaaa attatattca tttttgttga
    47581 tagttataat aataactatg aatccactca gaaaagtttt atttttgaaa attcgcttcg
    47641 caccgaccat aaagaaaact tattgtcagc aacttacatt tacaaatgaa gggcaaaaag
    47701 tcgctgattt tagttttttt tgacttttcg gaacagactc aatatttttc actaaatatc
    47761 actgttttat taaggattat ctaccaaatt tacgtcgaaa gtacgtaaat atacataaaa
    47821 tttaatgcaa aaaacaggct ttgttagctg tgctttattt gctgacagtc aagaaaaaaa
    47881 tattaaaaaa atccaacgga aaatactaaa agcatgaaaa gaaaagaact aatcaagcta
    47941 ttattgtgtg ttatattgct cactaacttc tttgttacta caaaaggcca aaagcttgac
    48001 agcaaaacta ctcctataca aacagtaagc ttagtagctg aaaaactaat aaaaacaacg
    48061 gactttagtt atcgattgga gttagcaaaa aaacgcaatt atttcgatga tgtaatatgt
    48121 gttgactttg ggcgcacttt tggcttggat aaaaaagcta cagccattgc agttacaacc
    48181 ctcgaaacac aaaatgagtc gattttgcct attcaattcg accatagcga tggttgcaaa
    48241 attacgctta ataataaaat tatttatgag catacaggca aacgcaaact caatgtaaaa
    48301 tcggatgagc gcgacttaga attaccactt aaactagatt tacaacttgc taaaggtatt
    48361 aattatttgc aaattgaact aagcacaaat ggttcagaat ggcgttttta tatgcaaccg
    48421 cctacagaca aaggggctat tctaaaaact caaattcagt acccaaaaat tgggttgcta
    48481 ccactatcta aaattgatgc taaagttgca gaaataagca actggctttt tatgggtcca
    48541 ttcgaaatga acgacaaaaa catccctgta ataaatggga atattgtttt tggcaaaatg
    48601 tacaatggac taaataatca gccaattact tggacaatac ccaaagtaga agtattaggc
    48661 aatgtaattg acccggcccc ttggggtaca acctatcagt ggaactatca taatgggggt
    48721 gtggcgtggg ccatgcaagt actttcagaa aaaacaggaa atccaaaatt caacgaatgg
    48781 gctacaaact tctgcgattt tcacattgaa ggattgccat ttgtcgatta tcaagtaaat
    48841 acactcaacg agttcaaatc agccaatcat ttcattctca atacgccact attggatttc
    48901 acgttggcgc catcgcttcc attcatccat cggctacaaa gcgaaaaaaa ctttgaaaac
    48961 cgagccgcat acgaaaaatt tatagcacgc atgctgaaat acgccaaaga ggaacaaata
    49021 cgcctgccag gaacaaatgt gtacacacgc acaacccccg aggtttacac tacatgggtg
    49081 gatgatatgt ttatgggaat accttttttg atacaggcgg cacaatatgt taccgaccca
    49141 aaagaaaagg caatgtttta cgatgatgct gctaaccaaa cattggaatt taaaaaatat
    49201 gtatgggaca atgatgctaa actatatatg catgcaaact actcacaacg ccccaatgta
    49261 aagctcccct attggtcgcg agctaacggc tggggcatat gggctacaac cgaagtactt
    49321 aaaggcttac caaaaaacca taaacattat aaagccatcc tcaaacattt ccaaactcat
    49381 gtggcttcgc ttgtaaaact acaagctcct aacggttttt ggcgtaatgt gctcaatcgc
    49441 cccgactcta aagatgaagt gtcgggaaca gcaattttca cccttgcaat tgcgcgtggt
    49501 ataacagaag gttggctcga cagaaataaa tatttgccta tagtacaaaa ttcgtggaat
    49561 gctatccaat cgcaaatcga tgccgatggt actgtgcaca atatttgcat gggcaccatg
    49621 tgctccgaag atgttgacta ttatatgaat cgtccatttt ttgttgacga cacgcatggc
    49681 acctttgccg taatatttgc aggcatagaa atgcaaaatc ttttggataa aattgaagaa
    49741 gaaaaagtat acaaaactaa tatggcacag attattgacc aaagcctaaa atttgctaca
    49801 caacaatcca ttggattagc cgaaaaacta atgagcgaac ccaaacgatt gcctcgcacc
    49861 attgataatg agggtaaatt gcttacaagt agcgaaaaat ggtggacaag tggctttttc
    49921 cctggtactt tatggtattt atatgaatac agcaaagatc caaaagtaaa ggcttttgcc
    49981 gaaaactatt ccgaaagggt taaaaacgaa caatatacaa ccgacaatca cgatgtcggg
    50041 tttatgatta attgtagtta tgggaatgcg tatagactcg acccaaagcc ggaatacaaa
    50101 ctaataatta taaatgccgc aaaatcattg tctacacgct ttaatcataa aataggatta
    50161 attagatcgt gggactgggg taaatggcaa ttcccagtca ttatcgacaa tatgatgaat
    50221 ctcgaattac tattcaatgc aactcggttg acgggcgata gtacgtttta taatattgct
    50281 gtaacacatg caactgttaa catgaaacat catttccgcg cagatgcgag ttgctatcat
    50341 gtagtttcat acgatacaat tacgggtctt ccagagcaca aaggaacatg gcaaggctat
    50401 agtcacaact catcgtgggc acgtggacaa gcttgggcat tatatggtta tactttgact
    50461 tatcgcgaaa caaaagatcc aaaattcctt gaaatggcaa cacgaattgc tgactttatg
    50521 ctgcatcatc ctcgaatgcc taaagatttg attccttatt gggattatga cgctcctggt
    50581 attccaaacg aaagccgcga tgtttcaact gcatcaatca tgagttcggc actcattgaa
    50641 ttaagtgatt atgtgaaagg agaaaaaagc agaacatatc ttgatgcggc acgcaaacaa
    50701 attgtagcat tgtcatcgcc caaatacctt gctcaattag gcgaaaacgg caactttata
    50761 ctcaaacaca gcgtgggttc gatgcctcac aaagcagaag ttgatgtacc actcacttat
    50821 gctgattatt attatattga ggcattgttg agatacagaa acaaactcaa aaaataacaa
    50881 tattaagtaa ttatggttct atcaataaaa caacagcata ataattaaca gtaatcaata
    50941 gcaatataat gaggactatc acacttatcc cactcttatt atcatgctgt ttagcttgga
    51001 ctcagagtga aaatatacaa ttaaaattga gtgagcataa cctaaaaata aagtattgta
    51061 tcaataattt taactacgat ctatacaaca aattaatcaa tacttatgag ttcgatattg
    51121 acttttctaa aaagaaacaa tcacttgaaa caaaaaatgc tatctgggaa gtagattcta
    51181 aattttcgga aacaagtgct ggcacatttg atgccataat tcaatttata ttattaaaag
    51241 gcactgaaaa caatgcatct gttgctgtca gtttcgaaat agacaattgg tcaaaaagca
    51301 attatgtttt gctacctgcc gctgcttaca atggaaaccg tttcgaatcc cgcaaaattc
    51361 cttactcccc aaaactcaac gattaccgtg acattggccc tctaaaacca ataattattt
    51421 cggacgtgcc gcgattaaat ttacacgaag gaccttctca aatacaagaa cgaagcggta
    51481 gtatgacaac ccctatgata ggctttcatt cctataatca aaaaaaagga ttgctattaa
    51541 caacaacgca agcaaatatt tggggcgact acggaataag cataactgaa actcgacccg
    51601 aaaccggcgc taaagcaatt attagcatta gctctccttt ggtgcgcgaa gggtttaaat
    51661 acgaaattgc taaccatgct atagcttcgc ccgataaacc tgctaatttc aaaataggcg
    51721 acacaataaa attcatcatc cgactacgct tgttcgaatc agaaaagctt tcaaatttat
    51781 atgaaaatta ttataaggcg aggtacgact taacacccgt ttcggtacct aattatattt
    51841 accctttctc cgagtgcttt aataccatag aacaaaaatt caatacacaa aattttgttc
    51901 ctgaatgggg atattatgcg gtggggatgc gcgagaattt tttacaagat tggcaaattg
    51961 gttggacagg cggcatgatc tctacctacc cgctactgtt ttcaaataat acaaaaacaa
    52021 tacaaaatgt tatagctaac tttaattggc tattccccaa tgggatatct ccttctggct
    52081 tcttttggga tagtggcgaa aagggaaata agtggtacgg cggtgatatt cgtcgacctc
    52141 agttcgagaa tctccatctt attcgaaaaa gtggcgatgg gctttattat ataattaagc
    52201 agttcgactt aatgaaaaaa agaaatataa aaattgaacc cgaatggcac aatggcgtca
    52261 aaactgtggc aaatgccttt gtaaaacttt ggcagaaatg ggagcagtat ggtaactttg
    52321 tggatagcag aacgggcgat gtagttgtcg gtggttcaac aagtggtgct atcgtccctg
    52381 ctgcattagc tctagcaagc gagtactacc aaaacccttt atatatgaaa gttgcaaaac
    52441 tttcggcgca aaaaatgtac aacgattatg tggccaaagg cattacttgt gggggaccag
    52501 gcgatgccct gcaaaacccc gacagcgagt cggcctatgc aatgctcgaa tcatttagca
    52561 ttttgtatga aaaaactggc gaaccacaat ggttagaata tgccaaagaa atggctttac
    52621 aatttgcaac atgggttatg gcttacgatt ataattatga ttatgatagt acatgtcttt
    52681 ttggtcgtat gaaaatgaaa acaaaaggta ctgtgtttgc taacacgcaa aacaaacatg
    52741 gctcgcctgg catttgcacc tactcgggct tagcacttat gcggctttat agagctacat
    52801 cccaaagtca tttccttgag ttactgcaag aaattacact ttctatacct caatatatgt
    52861 cgcataatac acgtcctatt gccgatatgc ctccaggttg gatcaacgaa cgtgtaagca
    52921 caactgattg gttcgaaggg attggcgaaa taaaagctgg ttcaacttgg gcagaaacag
    52981 ctatgctact tacagcaact gaactaccat ccgtttatgt cgacatcaaa tttggtaaaa
    53041 tatattgttt cgaccatttg caaacacaaa taattaaaaa caaccaaaac ttcatccaca
    53101 taaaggtaca caatcctaca aatttcacat cgcaattcaa tgtgtttttg gaaaacaagg
    53161 aaatgaagag taaagcttgg actgagaatc aattatttga ttgaataagt tcgaattaaa
    53221 agccggcgaa accaaaattc taaaatttgc aaaagcaaaa tgataacacc aagaatgaat
    53281 tataagcgaa aaatgcttct atttggagca ggtaaaattg ttcgttcatt tacaggcaag
    53341 tatttgcatg cgctcgttat gagattgttt ttgtcgacat aagccaacaa gtggtcgatg
    53401 caattcatgt tcgaaaatcc tacaaattaa aaataaaatc ggagcatgag tacgctattg
    53461 atgtaacaaa tgttagagca ttattgcttc ggatagtaaa gctgtaatca acaaaatgac
    53521 atcaacagat attatggtgt ttagaatgtt cattttgcag ggaaaacgca gaaaacttta
    53581 tatacactga gatacaaaac ttttgccaac ggtgcatatt ttgaaaaaaa tcaacaacaa
    53641 actggttctc aaaatcgctt taaataaaaa agatgagcct ggttttccgg cctatggaac
    53701 agctcatctg agtttgactt tccttttaaa ttggctaatg agtaatctgg gataaatttt
    53761 tgtgcgggct acaaaccttt gaataagcta ctatcccggc aattttttat accgcgtgtt
    53821 atgcagcgtt tttatttatt ctttttcaaa ttttaatccg tatgggattt tcactgtccc
    53881 ttttagataa ggattgataa aaatcttaat tgtatccgtt tcattttctg gtttgagaat
    53941 atataagtca agaatcggtc ccgtcccata actcaaaggt tctccggtat aacgattctg
    54001 caaggcaatt tttggctttt cataattggg attctctact gaaaaccttt gaatcaattg
    54061 aagtttatta cctttttctg ttctcaatct ggacaaataa tagtaagatg aacttatact
    54121 gttgtttaaa tccgcatttt taatcgtaac aggattttta gctgtataac catagcttaa
    54181 atctgttgtt ggttcaatct ttgagaaaaa tttcggatta ctcactacac atccagtcag
    54241 caataatcca atccacaagc taaatcttaa ttttttaaat aatcttttca tcagaatata
    54301 atttattttt taaaggtctg atggaaccca catgtctgaa tattatattc attcgtgttt
    54361 gtatatgcaa tacatgtggg tttcttagtt agactcacaa ttttaggact ttttgtattt
    54421 ttcctcgtct cctcatttcc ttgtttcctt gtttccttca gtcaccctta aaaaacaaac
    54481 cttcaatctg gtcgtcgaga taaaccattt cgaattccac ccaaccaacc aggccaaacg
    54541 aagtttttac caaatatcta aaatattctg ccggatctgc caaaagaact tcaatagaag
    54601 aacctgctga gagttttgcc acttcctttg tcatattttc atctgaataa agcgtcagcg
    54661 gaattttggt tttacttttt aaaccaacat aataaaaggg ttgtctaacc tccttaactt
    54721 catctttttg tattctgaac ttttgccgtg tattaaaagt attattggta tgtccatcaa
    54781 tataaaaaaa accatttgaa gttatgtata aatgcaatgc accaatccct atgattgcac
    54841 tgtcgggatt tgatttattg aaaatataaa aagccggatc ggctgacatg cccggagtat
    54901 aaaaaacaag atatttttca aaacaatctg cttcgagaca agtttcaaga acgagtatat
    54961 catcggcatc gtactcatca ttacttcgga atggattatt cacaggtaaa gctgaacgtt
    55021 ttttctgtat cagagtggat tttgaagcat cgtatttcac atacacatcc atatctgcat
    55081 ccacaactac ttttttaagt tgcggaaaat ggttttcctg cgcataaatc tgcaaagaaa
    55141 aagttatcag aaggaacaaa agcgttgatt ttttcatgcg tttatatttt taagtgacta
    55201 ttgagcacaa aataaatttt aaaaaccact actatccgaa actttagaaa ttaaaaatgc
    55261 ctgagaattt catatttttg t
//
LOCUS       DAOTAX010000004        55213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_482_length_55213_cov_32.299068, whole genome shotgun sequence.
ACCESSION   DAOTAX010000004 DAOTAX010000000
VERSION     DAOTAX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 55213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55213
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_482_length_55213_cov_32.299068"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1949)
                     /locus_tag="PLM04_00850"
     CDS             complement(66..1949)
                     /locus_tag="PLM04_00850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HON01663.1"
                     /translation="MEEIVEKVTLTEYGDSNIITLEGLEHIRRRPGMYIGKLGDGSHP
                     DDGIYVLLKEVMDNCIDEFRMGAGKTIEVQIVNDMVQVRDYGRGIPLGKMIEAVSIMN
                     TGGKYDSKAFKKSVGLNGVGTKAVNALSEKFIAQSFREGQTRKAAFEKGKLTFDSGIV
                     ESDAERGTLITFTPDHEMFDGYEYRDEVVETMLRNYVYLNAGLTIVYNGKKIISRNGL
                     TDLLNENMTVPALYPIIQLKGEDIEVAFTHCDQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVAKTIKEFYSKNMEYTDIRNGIVAAIAVNIEEPVFESQTKTKLGSRDIAPGGISVN
                     KFISDFIKTELDNYLHRNLETAEILLQKIQDSEKERKAIAGVTKLARERSKKVSLHNK
                     KLRDCRVHYNESKPGAEESSIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNCFGLT
                     KKVVYENEEFNLLQAALNIEDGIEGLRYNKIIVATDADVDGMHIRLLLITFFLQFFPE
                     LIKKGHVYILQTPLFRVRNKKEKIYCYSEEEKIAAVKRLGATAEITRFKGLGEISPDE
                     FKNFIGKDIRLEQVSLRREDAVSELLEFYMGKNTSERQNFIIENLVIEEDKVEEEIEL
                     KIE"
     gene            complement(2018..3217)
                     /locus_tag="PLM04_00855"
     CDS             complement(2018..3217)
                     /locus_tag="PLM04_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HON01664.1"
                     /translation="MIIAFACSGTQLLAQNVKQLEEERKQTLQKLETTSKMLDETKKW
                     QKSSLNKLSLLNKNINERTHLIKTMSKEISQLDVEIKTMGQEIEQLERRLENLKNDYA
                     RLVQEAYINRSIYSKIMFVLSAETFDQSLRRIRYLKEYSDYRKHQVSEIEKVKAQIEQ
                     KKDSISKHKSTLVVVVKEKEVEATKLSQEQKKEKKLLTDLQKKEKNLRKELDAQQKKA
                     NDLNKRIESLIAEEIRKAENLNKSKGKSTTSKVSTLTKEEVLLSGNFEKNAGRLPWPV
                     SNGFISGHYGIQPHPVLKHVTTNNKGIYIQTPANSTARAVFDGVVTQLFSVPGSNNAV
                     IIQHGEYRTVYANLTDIYVKIGDKVSTKQAVGKIFTDSEKDNKTELYFQIWKNKNLLD
                     PEKWLAR"
     gene            complement(3307..4104)
                     /locus_tag="PLM04_00860"
     CDS             complement(3307..4104)
                     /locus_tag="PLM04_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HON01665.1"
                     /translation="MKKILSIFLMILVLLSSCKTKKQVQQPSYFPKKLTTEKVIEQLN
                     ALDNIQTAEIKKMNIYLKMKDREFNVTSSMTIKKDSAINISIQPFFGMELFKLEITPN
                     NIFIFDKVNQTYYTTDFQYLNLRTGLGIDFYHLQALLTNRYFCLARQKDIACSILAQN
                     NHKISFGFDNETLMQTMSINPDFNITEILLVDKINNLSFNALYENFALINGIIFPRQI
                     TYMLKGQKHLSSAKITIDRVKFNQQVEFIPTDTRRYKKGSFDQLFKK"
     gene            complement(4149..5915)
                     /locus_tag="PLM04_00865"
     CDS             complement(4149..5915)
                     /locus_tag="PLM04_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON01666.1"
                     /translation="MHKKIFLFPILIFLCLLTISCSSTRKSVTREKNFRSNKLTPEQQ
                     SQFDYYFYEALRLKNNQQLDEAMETFLFCRQIDSLDAEVQSQLGVLYANIGLIDPALK
                     ALKKSVELQPTNWWYSMTLLNIYSQQKNWKDAIALANEMQKIYPEKEEIYSTLAQLYK
                     ASGDIDKAIDAYNRLENLTGIDESISFAKFSLYAQQNKVKKAIAEIDRLIEKYPTENR
                     YKVLRGDIYLQLNNLEQAFAIYEQILKEEPQNPFVYISLSEYYKKINQPEKATEAIVS
                     ALKNEQLDVDTKIEILGQYIDNLIKDKEKLDETEALFKLLVDRYPLEEQVHAYYALFL
                     EYQERIPEAISELETMLNINPKNLQTWLKLIQIYWSDKNFEKIYVTTERAIEVLPEEP
                     ILYFYQGIALYQLNDYQASLQKNKKALLYIKDQQKALKSDVYAQLGDTYFKIGQKDSA
                     FIAYEESLKANPENIYVMNNYAYYLSLEKKDLKKAERMSAKTVEKEPNNATFLDTYAW
                     ILYQQGNYSLAKFYIERAIDNLKDGEDNGIIYEHYGDILWMYGGNDEKALEMWIKSYE
                     LGNQTEELKQKIENKGFDRQNK"
     gene            complement(5986..6417)
                     /gene="dut"
                     /locus_tag="PLM04_00870"
     CDS             complement(5986..6417)
                     /gene="dut"
                     /locus_tag="PLM04_00870"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HON01667.1"
                     /translation="MKIKIVNLSKHPLPSYATLLSAGMDLRANIDEPVVLKPLERKLI
                     PTGLFIELPEGYEAQIRPRSGLAIKHGITVLNSPGTIDADYRGEICIILINLSNDDFV
                     INDGERICQMVIASHEHAEWEEVKMLNDTDRGNGGFGHTGR"
     gene            complement(7036..8262)
                     /gene="pepT"
                     /locus_tag="PLM04_00875"
     CDS             complement(7036..8262)
                     /gene="pepT"
                     /locus_tag="PLM04_00875"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HON01668.1"
                     /translation="MTLTDRFLRYVSFTTTSDELTNMTPSTPGQMVFAQHLVKELKSF
                     GLKDAEVDKNGYVMATLPANTDKKIPTIGFIAHLDTSPDMSGKNVKPQIIENYDGKDI
                     LLNEEQLIVLETEKFPEILQYVGQDIIVTDGTTLLGADDKAGIAEIISAVEYLLRHPE
                     IKHGKIRIAFTPDEEIGKGADNFDVKKFNADWAYTIDGGEIGELEFENFNAASAKITF
                     HGLNVHPGYAFHKMKNSIRIAHQFVAMLPRHETPEHTEGYEGFYHLTSIEGTVEKTTM
                     HLIIRDHDRDKFEKRKKELQHLANKINAEFGENTAVIELRDQYYNMREKIEPVFHIID
                     LASDAMKEVGITPKIVPVRGGTDGARLSYMGLPCPNIFAGGMNFHGRFEYIPIQSMEK
                     AMMVIVKIVELAAKKY"
     gene            complement(8465..8537)
                     /locus_tag="PLM04_00880"
     tRNA            complement(8465..8537)
                     /locus_tag="PLM04_00880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8502..8504),aa:Lys,seq:ttt)
     gene            complement(8682..9542)
                     /gene="atpG"
                     /locus_tag="PLM04_00885"
     CDS             complement(8682..9542)
                     /gene="atpG"
                     /locus_tag="PLM04_00885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HON01669.1"
                     /translation="MSSLKELKSRIQSVKSTQKITSAMKMVSSVKLLKAEKAVYNFLT
                     YRETLTDILRNYLSSQIVDRSPFREIRHVNHVGIVVFSSNSSLCGNYNSNIIKRLEQS
                     ITTHRDVRKEDILIFPIGKKVEDFCKEKNLAIAGEFQNVAAHPDIENTTQIADTLIQL
                     FLQHKIDRIKVIYFHYVNKTTQIMIAETFLPIEINYRKKGEFRNDYLIEPSIEEIEQQ
                     LIPLALHYHIYTAALEAYASECAARTLSMQAATENAENLLQQLSLEYNKMRQESITNE
                     ILDLIGGSLQ"
     gene            complement(9590..11182)
                     /gene="atpA"
                     /locus_tag="PLM04_00890"
     CDS             complement(9590..11182)
                     /gene="atpA"
                     /locus_tag="PLM04_00890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HON01670.1"
                     /translation="MSDNKTIKVSEISELIKKQLQQIDIESYYEETGKVLEVGDGVVR
                     IYGLTNAEANELLEFENGVKAIAMNLEEDNVGAVLLGPTHLIKEGFTVKRTGRVASIK
                     VSEKMLGRVINPLGEPIDGKGKIDGEFFEMPLERKAPGVIFRQPVEEPLQTGLKAIDS
                     MIPIGRGQRELIIGDRQTGKTSIAVDTIINQLDNFKAGDPVYCIYVATGQKGSTVATL
                     FNTLKDFGAIPYTILVVATAADSAALRYLSPYAGAAIGEYFRDTGRHALVVYDDLSKQ
                     AIAYRELSLILRRPPGREAYPGDIFYLHSRLLERAAKIINQQEVAKQMNDLPECLKGK
                     VKPGGSLTALPIIETQAGDISAYIPTNVISITDGQIFLDTDLFNEGNRPSIDVGISVS
                     RVGGNAQIKPMKKVAGTLKTDQAQFRELEAFTKFGSEMDAVTAMTIDKGQKNTQLLIQ
                     SLHRPLSVEKQVALLYCGTHGLLANIPLHKVKEFEENFINLLDYSYKEKILNEIKKGN
                     FDEKVTSSIEEAAEILKKQMNY"
     gene            complement(11224..11775)
                     /locus_tag="PLM04_00895"
     CDS             complement(11224..11775)
                     /locus_tag="PLM04_00895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HON01671.1"
                     /translation="MQTGLIASRYAKALYEFASEMNAQEIVFANFQTLEKSFSANAEL
                     AQALKNPLLTRETKKQLIQFACGNKSDFILERFMDVLLQNRREYLLRNIGLKYSDYYY
                     ENKNLALVKLTTASPMDAETEKKLISQIKTYIKKELKVEKIIAPEIIGGFIIEIGNRR
                     WDGSLATQLKKIQKELNENILLT"
     gene            complement(11849..12355)
                     /gene="atpF"
                     /locus_tag="PLM04_00900"
     CDS             complement(11849..12355)
                     /gene="atpF"
                     /locus_tag="PLM04_00900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HON01672.1"
                     /translation="MSLLMPDFGLFFWMMLSFGIVVFILVKFGFPAILQMIEKRRSYI
                     EESLLEAENARKELEEIKTEAKKIQDEAFLNRKKILDETTEIKNALIKEAKEKAFIEA
                     EKIMEEAKKQIRYEKMMALQEIKQQVAEISIAVSEKLLQSKLNDKPAQMEIINRMLEK
                     MDIGKFGN"
     gene            complement(12466..12714)
                     /gene="atpE"
                     /locus_tag="PLM04_00905"
     CDS             complement(12466..12714)
                     /gene="atpE"
                     /locus_tag="PLM04_00905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HON01673.1"
                     /translation="MLLSILLQVAASDALAKFGAALGAGLAAIAAGIGIGKIGGSAME
                     SIARQPEMSSDIRMNMIIAAALIEGVALFAIVVCGFIL"
     gene            complement(12801..13892)
                     /gene="atpB"
                     /locus_tag="PLM04_00910"
     CDS             complement(12801..13892)
                     /gene="atpB"
                     /locus_tag="PLM04_00910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HON01674.1"
                     /translation="MKCCFVYIICFLAFSPFKILAQNNNYEGKRKEIDVREIILTEVS
                     DSYEWYITTWKSKSISIPLPVILHSNNSGWHVFMSSKFEEQPVYKGFYIAKEGKYKDK
                     IVELDENGKEIRPLDISLTKTATALIFNSVLLIILIMSIAHYYKKDGIFPKKGFVILM
                     EMLIVDIYEGVIKPSIGNDYRRYAPYLLTVFFFIFLNNLMGLIPFFPFGATVTGNIAI
                     TFTLALFTMLIVNFSGTKEYWKEIFWPEVPAWLKVPVPLMPAVEFIGIFTKPFALMIR
                     LFANIVGGHSIVGGLVCLVFVTVKLGTTINVTMSALSVIFTVFIDLIELLIAYIQAYI
                     FTMLSAVFIGLARVEPSHKQAAKEVVKQV"
     gene            complement(13889..14284)
                     /locus_tag="PLM04_00915"
     CDS             complement(13889..14284)
                     /locus_tag="PLM04_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01675.1"
                     /translation="MKNNLLFSLVFFSLLTLLLGLFFWLLLKKFSSTTIENYFLLPLL
                     FYFSGLTLMLVLQKFSYISSTKKIYLFLLLKLSKMILWGIVAFVYLLVFRTERRMFGL
                     LYGAYYLIYLIFETACFYMNEKRQVRQSK"
     gene            complement(14274..14528)
                     /gene="atpC"
                     /locus_tag="PLM04_00920"
     CDS             complement(14274..14528)
                     /gene="atpC"
                     /locus_tag="PLM04_00920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HON01676.1"
                     /translation="MNLKIVLPNKLIYDGEISSVTVPGKGGVFTILSHHAPLIAILGK
                     GNVTFRFKNGVNNVFINGGLIELNNDKVVICAEQIPENEK"
     gene            complement(14525..16072)
                     /gene="atpD"
                     /locus_tag="PLM04_00925"
     CDS             complement(14525..16072)
                     /gene="atpD"
                     /locus_tag="PLM04_00925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HON01677.1"
                     /translation="MLSIQGKVWQIIGPVVDVFFPLEEKNEFELPPINNALSISLSNG
                     KNLILEVQQHIGENIVRTIALDSTDGLSRGLPVSDLGSPISIPVGNQVKGRLMNVIGE
                     SIDGLSPLGRVESAPIHKEPPKFDELANNDEILYTGIKVVDLLAPYVKGGKIGLFGGA
                     GVGKTVIIMELINNIAKWHKGFSVFAGVGERTREGNDLLREMIEAGVIRYGEEFNKAM
                     QEGKWDLSKVDYEQLKKSQATLVFGQMNEPPGARYSVALSGLTVAESFRDSDESENHD
                     ILLFIDNIFRFTQAGSEVSALLGRLPSAVGYQPTLATEMGLLQERITSTKSGSITSVQ
                     AIYVPADDLTDPAPATTFSHLDATTVLSRQIASLGIFPAVDPLDSTSRILDPLIVGQE
                     HYETAQRVKQILQRNKELQDIIAILGIEELSEEDRLTVQRARKVQRFLSQPFFMAEHF
                     TGVPGVMVPIEETIHGFKLILDGMLDDLPEMAFLNVGTIKDVLNKGHALLREAHNLNK
                     TFKIEDQ"
     gene            complement(16211..17998)
                     /gene="lepA"
                     /locus_tag="PLM04_00930"
     CDS             complement(16211..17998)
                     /gene="lepA"
                     /locus_tag="PLM04_00930"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HON01678.1"
                     /translation="MNNIRNFCIIAHIDHGKSTLADRLLEYTKTVVGKDLQEQVLDNM
                     ELERERGITIKSHAIQMNYQYQSIDYILNLIDTPGHVDFSYEVSRSIAASEGALLVVD
                     ATQGIQAQTISNLYMALDHNLTIIPVINKIDMDSARPEEVEDEIVELLGCSREEIIRA
                     SAKTGQGVEEILNAIVERIPVPQGDPEAPLQCLIFDSIFNPFRGIIAYFKIVNGTLNK
                     GDWVKFVATGKDYYAEEIGVLKLEPQPVQTLSAGNVGYIISGIKTSKEVKVGDTITHV
                     KRPCQKAIAGFEEVKPMVFAGVYPIDTEDFEDLRASIEKLQLNDASLTFEPESSTALG
                     FGFRCGFLGMLHMEIIQERLEREFNINVITTVPNVSYKIYTKKGELIEVHNPSEMPDP
                     TLIDRIEEPYIRASIITPMEYIGPIMTLCLGKRGELIKQEYISGNRIELIYDLPLSEI
                     VFDFYDKLKSISKGYASFDYHMNGYRASKLVKLDILLNGEPVDALSSLIYADNAYSLG
                     RRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFLAVLKLD"
     gene            complement(18203..20026)
                     /locus_tag="PLM04_00935"
     CDS             complement(18203..20026)
                     /locus_tag="PLM04_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HON01679.1"
                     /translation="MLQKIIQFFKIYLLILFVFVLQKPFFILYNHQIYSNLSFQDLIL
                     IILHGIKLDASMSAYLTILPGIFLIVSLWAKPSVHEYFQKIYFGLISFIISVVFVVDA
                     ALYPFWGFRLDSTVLFYMKWPKEALASIDIWMIVVGLLAIILIALTLYAIFYFLLIKI
                     NKESKQVKNKLSTACLLFLFTCLLFIPIRGGFNVSTMNVSKVYFSPKNELNHAAINPM
                     FNFLESAFKTQNFDKQYRFMSEEEAQKEFNNLIDQPVQEELPKLFTSPKPNVIFVVVE
                     SFMSNNLEPLGGFPGIAVRMNEFCDEGILFTNFYANSFRTDRGLISIFSGYPAQPTTS
                     IMKYPKKSQSLPSIPKTLKTAGYDLQYYYGGDVDFTNMRAYLIAQGIDKMVVDKDFPL
                     KDRTTKWGAHDHIVFQRLTNDLKKEQKEPFMKILQTLSSHEPFDVPFYKNSDPYLNSV
                     QYTDSCLGAFVDEFRKTKYWENTVLVLVPDHAMHYPKNILLSSVERYQIPLLIIGGAV
                     KSPMKIDTYASQIDIAATLLYQLELPHQDFTFSKNILNPASPHFAFYTFPNGFGFLTP
                     ENHYVFDCSTNKVLWNWGQPNANKKKGEAYLQMLYNDLSKR"
     gene            20597..22651
                     /gene="htpG"
                     /locus_tag="PLM04_00940"
     CDS             20597..22651
                     /gene="htpG"
                     /locus_tag="PLM04_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HON01680.1"
                     /translation="MTKGTIGVTSENIFPVIKKFMYSDHEIFLRELVSNAVDATQKLK
                     TLASVGEFKGELGDLKIRISVDKKKKTITISDRGIGMTAEEIDQFINQIAFSSAEQFL
                     EKYKNDAQSIIGHFGLGFYSAFMVSKKVEIFTLSYKEGAVAQHWSCNGDPEYVLEDTI
                     KSDRGTDVVLHLDDDNLEFLEESKIRELLLKYCRFLPVEIAFGKKKEWKDGKYVETDE
                     DEIINNTQPAWIRKPSELTEEDYLNFYRELFPYNEDPLFYIHLNVDYPFHLTGILYFP
                     KIKNNIDIQRNKIQLYCNQVFVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSFLQND
                     SNVRKISNHITKKVADRLQEIFKNDRNQFEQKWDDLKLFIQYGMLTDEKFYERAEKFA
                     LFKNVDGKYFTYEEYKTLIGENQKDKNGDLIYLYATNAEEQYSYIQHAKDKGYDVLLM
                     DGQLDIHLIGHLEQKWEKTRFVRVDSDVIEKLIQKEDTANVSLSEAQREALTAIFNSQ
                     LPPMEKTNFIITFEQLGPNVNPVLITQNEFMRRMKEMSAMQGGMMNFYGEMPDSYNLV
                     VNTTHPLIEKLLKDEETACAEELAPIEAELLELSNKLQQLKENHKNKKEEEIPAEEKE
                     ELTTLEKRIDELKEKRKSVLAAFASNYKTVRQLIDLALLGNNMLKGEALANFVKRSID
                     LM"
     gene            22793..23500
                     /locus_tag="PLM04_00945"
     CDS             22793..23500
                     /locus_tag="PLM04_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HON01681.1"
                     /translation="MRKKTTLFFAFLPLAAMAQNFQLHYDFGQGRHYVTTTFEMFKPD
                     DWGNTFFFVDYDFNMDQDHNASLSYMELARCFSLGKTSPFSVQVEYNGGLFAMEGSAY
                     PIQNAFLIGLDYGWHNNNFDRFLNFKVLYKNIVGKHPLSFQLTGVWDLSFYNKRISVC
                     GFADFWWEDNLNFTDAAGKNLDSPVLTRYVFISEPQFWYNITPHLSAGSEIEIAANFS
                     SVYGLKICPTLGMKWNF"
     gene            23654..24631
                     /locus_tag="PLM04_00950"
     CDS             23654..24631
                     /locus_tag="PLM04_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HON01682.1"
                     /translation="MKNFLVFIVMAGFLVSCHSATQKSANEEKVINIPVFDSDSAFSF
                     VQRQVDFGPRVPNTPAHDACANFFVATLKSFGAQVTTQQMDLRTFDGTLLHAVNIIAS
                     YLPESETRILLFAHWDSRPWADNDPNPKNREKPVLGANDGASGTGVLLEIARQLGKNP
                     ANVGVDIIFFDAEDWGASNDDQTSNTENSWCLGAQYWAKHPHKQGYHARYGILLDMVG
                     APQATFYREQYSEYYAPHVVEKVWSQAQNLGFGQYFINQKGGVIMDDHVFVNQMAGIP
                     SINIIHFDPNTSSGFGNYWHTTHDDMSNIDKNTLFAVGTTLMNVIYNEK"
     gene            24635..25717
                     /gene="proB"
                     /locus_tag="PLM04_00955"
     CDS             24635..25717
                     /gene="proB"
                     /locus_tag="PLM04_00955"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HON01683.1"
                     /translation="MEFRFKRIAIKIGSNVLTKSDGTLDTESMSNLTNQIATLYHQGI
                     EIVVISSGAVASGRSILGLNKKMDIVDQRQLYSTVGQAKLISLYWDLFAKHHITIGQV
                     LTTKQNFDSRRNYLNQRNCMRVMLENKVLPIVNENDAVSVSELMFTDNDELSGLIASM
                     MGMEALFILSNVDGIYRGSPSDPKAEVIREIKRGQDISDFIQTTKSSFGRGGMQTKVS
                     IARKIADQGIEVFIANGRKENILVRLIDAADDVVCTHFIGYRGEVSSVKKWIAHSEDF
                     AKGEIKINTNAVKALMGDKAVSLLPIGVTEIIGDFEKDDIVKIVDENGQSIGVGKVRY
                     DSKKARELIGKKYQKPLVHYDYLYLE"
     gene            25887..28151
                     /locus_tag="PLM04_00960"
     CDS             25887..28151
                     /locus_tag="PLM04_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HON01684.1"
                     /translation="MRNIKIRLKIETIAVALLLTGCNTTKFVPEGEYLLDKVNINIDT
                     RNVKKIELKEYLRQTPNSSVFGLFRTQLGIYSLAGKDTSKWINKTLMRIGEEPVIYNP
                     ALTEISSQQLQLFMHNKGFLRAKVENKVTLKDKKAEVEYQILAHQPYRIRNYSVQLNH
                     PELQAIAADTSRSLIRPGMLFDVDMLDAERERITSRFRQTGYYNFSKDFLVYSADSIE
                     KTHQVDLSMQLRDYLNDKNDSLSEIIFRKFYVRNVYFEINPTDLPSLNQPMEKDTVWF
                     RNFAMITPHKQIIKIDALVQNTYIDPQALFNDANIEKTYSGLNSLGAIKFVNIGFEQV
                     DTGLLDCHISIIPGKAISLSAEVEATYTDGYWGGATNLNLVNRNIFKGAETLSLQVHG
                     AYELQEKVWAQEWGGQIGLQFPRVVLPFASYDFKRNLRANTEFSGSYYYQFRPGEFRT
                     SNLGAGMRYFLNRRQYTHIFDLFEVNYVYFPEIFPAFRDSFLTTGIFNSYNYENHFIM
                     RMGYSGSSTNFNANQPLRNYRTMRYSIETAGNILYGLDHLLGSRPAEDGNYRLLNIAY
                     AQYFKTEYNLTYHQIFDENNRFVYHAGLGIAIPYGNADAVPYEKRFFSGGANGVRGWS
                     ESTLGPGVYKRINTQRRDFNQTGDIKLDMNMEYRGKLFWLLESAVFMDAGNIWTIKDY
                     DTQQGGLFKWNSFMNQIAVAYGVGLRFDFSFIVARLDMGVKLYDPVLKRSDRWRVSPQ
                     WNDVAFHIAIGYPF"
     gene            complement(28183..28836)
                     /locus_tag="PLM04_00965"
     CDS             complement(28183..28836)
                     /locus_tag="PLM04_00965"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HON01685.1"
                     /translation="MNKIPISKIKLYPDKTNRKQPSLIRSFSSFQHECGCDEAGRGCL
                     AGPVVAAAVILPPDFICPQLNDSKQLSPLLREKFRKIIEKEALAWSVASVDNQEIDRI
                     NILNASILAMHKAIDGLQIKPQFIVVDGNRFKPYADIPFQTIVKGDSKYLSIAAASVL
                     AKTHRDELMEILGEKFPEYEWKKNKGYPTARHREMIRRYGCTEYHRLSFRLLKEELF"
     gene            complement(28784..29326)
                     /locus_tag="PLM04_00970"
     CDS             complement(28784..29326)
                     /locus_tag="PLM04_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HON01686.1"
                     /translation="MNTLDLVILVTVFFGIIQGLFRGLVKELTSFAVIIVGIYGAKLL
                     NPMIAKMLESVASFSPQMARPLAYLFSFILLAIAMFLLAQLFEKTLSIIQLGGLNKFL
                     GAIVGGLKFLLVLSILLNVFDAMDERFHLINKATKEESLTYSLVLKAGPELWKELKDK
                     QSTNEQNTHQQNKIVPRQNK"
     gene            29585..30685
                     /locus_tag="PLM04_00975"
     CDS             29585..30685
                     /locus_tag="PLM04_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01687.1"
                     /translation="MKTKILGLAALLLFATQTPAQSTFTVEARNYDISDNLDLRAVAS
                     IFAESRDLSDFEMRLNDARYQISNLDLNNDGWIDYLRVVETSGGNLHLVVIQAVLDRY
                     TYQDVASIVVEKRNSPQVYVQIIGDPYLFGVNYVIEPIFVRQPVIFSFFWSKNYRPWH
                     SPYYWGHYPKYYRPLRPVEVNIYLGHVHHIIPRDYKFHYHNSVNIYYRDAYVRLHQTV
                     RRNDFERTHPERGFNSRNKNVANKAELNNSSRRSSTEQERRIVNQDNGSRRISTSSSD
                     SRYNNSENNSSRRSQNTGRVETQNNARSISNTGRSVETRTSSQNEKNSSRTETLRRSE
                     NSTVNTSQRTSENNSRGTRSTNSNSENRSGRR"
     gene            complement(30769..31833)
                     /locus_tag="PLM04_00980"
     CDS             complement(30769..31833)
                     /locus_tag="PLM04_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HON01688.1"
                     /translation="MKFESILLPGIEAKRPIVIAGPCSAETEEQVMETARGLASIGIK
                     IYRAGIWKPRTKPGGFEGVGEKGLPWLKRLKSELGLYTTVEVANAKHVRAALAHDIDI
                     LWIGARTTANPFAVQEIADALEGVDIPVLIKNPVNPDLELWIGAIERIYNAGIHKIAA
                     VHRGFSSYDKKIYRNLPQWHIPIELRRQMPDLPIFCDPSHIGGKRELIAPLSQQAMDL
                     NFDGLMIESHCNPEKAWSDAAQQITPQVLEFILESLVIRDTMQTTENLSALRRQIDEL
                     DNNLLELLAHRMRVAEEIGRYKKEHNMPILQAQRYDEILHNRVEQAIEMGMDGEFMKT
                     VLIAIHEESIRHQMEIMEKS"
     gene            complement(31885..33057)
                     /locus_tag="PLM04_00985"
     CDS             complement(31885..33057)
                     /locus_tag="PLM04_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HON01689.1"
                     /translation="MLKQLKMEIEPARRVNNVSEYYFSTKLKEIDAMKAAGKDVINLG
                     IGSPDLPPSEATIEALCKEAHKPNVHGYQSYIGIPELRNGFAEWYKKWFGVELNPNTE
                     IQPLIGSKEGILHISLAFLNPGDGVLVPNPGYPTYTSVSNLCEAKVIPYDLDENHEWQ
                     PDFDALEKMNLEGVKLMWTNYPNMPTGANASVELFEKLVAFGKKHNIIICNDNPYSFI
                     LNDRKLSILSVDGAKDICIEMNSMSKSHNMAGWRIAMLASNRQFVEWVLRVKSNVDSG
                     MFRPMMIAAAEALKNPKEWHDQINVVYARRRNIAFKILDLLGCTYDTRQVGLFVWGKI
                     PDRYKDAGELADEILYGKNVFITPGFIFGDKGNRYVRISLCCPEPTLEEVVRRIEH"
     gene            complement(33085..33918)
                     /locus_tag="PLM04_00990"
     CDS             complement(33085..33918)
                     /locus_tag="PLM04_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HON01690.1"
                     /translation="MKRVAIQGISGAFHEIAAREYFKGEEIEIIPCETFKDVFKAIEA
                     DKSLLGIIAIENTIAGSLLQNHNLLRESGCLVVGEQKLHIEHHLVALPHQKLEEIEEV
                     HSHPIALMQCEDFLESHKHLKAVESEDTALSAKEIADGQIYKRAAICSLFAAEKYGLE
                     ILERNIETNKRNFTRFLIVAEPTLALEMTKNDEINKASLVFTLPHVEGSLSQVLSIFA
                     FYHMNLTKIQSLPIIGKEWEYQFYINLTFNDYSRYKQAIDAILPLIDEFQIMGEYHAE
                     N"
     gene            complement(34187..36814)
                     /gene="alaS"
                     /locus_tag="PLM04_00995"
     CDS             complement(34187..36814)
                     /gene="alaS"
                     /locus_tag="PLM04_00995"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HON01691.1"
                     /translation="MTSQEIRQSFLDFFAEKGHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPVKYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEYLTEVLKIDKSRLYVTVFEGSPSEGLERDEEAAAIWSQFLPADRIINGS
                     RKDNFWEMGETGPCGPCSEIHIDIRSEEERAKLDGLSLINQGHPLVIELWNNVFMQYN
                     RKADGTLEELPAKVIDTGMGFERLCMVIQGKTSNYDTDIFQPIIREIGKISGTEYGKD
                     EKIDIAMRVVSDHIRAIAFSIADGQLPSNMKAGYVIRRILRRAVRYGYTFLHQHAPFM
                     YRLIDVLCETMGKAYPELISQKTLIEKVIKEEEESFLRTLETGIRLLERKMEEAQLEN
                     KKEISGADAFVLYDTFGFPIDLTELILRENNFTVNMNEFHAEMQKQKERARNAANIET
                     GDWIVLREGDSHFVGYDCTSCETNILRYRKIKQKNQEFYQLVLSVTPFYAEMGGQVGD
                     SGWLVGENEKIEILDTKKENNLAVHIAKQLPSEITGTFTAVVHLENRQATSCNHSATH
                     LLHEALRTVLGTHVEQKGSYVGPDSLRFDFSHFQKMTKEEIRAVEALANRKIRENLPL
                     NEMREVPVSEAKEMGAMALFGEKYGETVRVIQFGSSKELCGGTHVKATGEIGMVRIIS
                     ESSIAAGIRRIEAITGKAVEKLMDEQQDILSETREILNNPGNLVNAVRKLMEENTELK
                     KQVNTFQQEKLLQLRDRLMAEAKITNGIHVVRYEGNLTADQAKTLAFQIKNLQNENLF
                     FIAGNVMDGKPSLTLLLSDDLVSRGLNAAQIIREIAKEIQGGGGGQPFFATAGGKNAA
                     GLSAAMSKALKFIDEFPSK"
     gene            37016..>37968
                     /locus_tag="PLM04_01000"
     CDS             37016..>37968
                     /locus_tag="PLM04_01000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HON01692.1"
                     /translation="MAKKKYHFNPDTLSYEPIENSLWFKIRRFLFHAFTGIATGTVFF
                     FILASTVQSPEERELNAEKNKMEAQYKVLQRQFNEVQDVLTDLEERDNNLYRVVLQAE
                     PIPFTVRKAIASNTDYYNQLYDMTNSEIVVSTTKMLNTLKKQLYIQSRSYDELVNLTK
                     NREKMLQCLPAIQPVLNKDLKRMASGYGWRIDPVYHTRRFHEGMDFVAPTGTDVYATG
                     DGTIVNAGWEQGYGNCIVINHGFGYETLYAHLSTIKVRPGQSVKRGDIIGLVGSTGKS
                     TAPHLHYEVHYKGQVMNPQNYYFLDLSPEEYDRMVQMSNNFG"
     assembly_gap    37969..38068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38170..39051
                     /gene="murI"
                     /locus_tag="PLM04_01005"
     CDS             38170..39051
                     /gene="murI"
                     /locus_tag="PLM04_01005"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HON01693.1"
                     /translation="MNELQGNKNYSLTKLELSSLPGPIGVFDSGYGGLTILEKIRQRL
                     PHYDYLYLGDNARTPYGTRSFEIVYKYTLECVVRLFELGAHLVILACNTASAKALRTI
                     QQNDLPKIDPRRRVLGIIRPTVEIIDKLTHNGHVGLLATPGTVQSNSYGLEIQKLYGN
                     AITLSSEACPMWVPLIENFEHNSPGADYFFQKNINHLLQTDPQIDTVILGCTHYTLLQ
                     DKIKNLLPPNINIVSQGEIVASSLDDYLNRHPEIDALCTKNASCRFLTTESVEKFSAS
                     ATIFLNEPVLAEHIDID"
     gene            39317..41476
                     /locus_tag="PLM04_01010"
     CDS             39317..41476
                     /locus_tag="PLM04_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HON01694.1"
                     /translation="MKVYQPNEIKNISLLGSSGSGKTTLVEAMLFEGGIIKRRGTIAN
                     QNTVCDYFPVEKEYGYSVFPTLAQIEWHQHKLNIIDCPGSDDFIGAVVTSLNVTDMGL
                     IVVGAQYGVEVGTNNHFSYAEKYNKPVMLVVNQLDHDKADYEHTLEQLKENFGNKVIQ
                     LQYPLQTGSGFNSLVDVLQMKMLRFKTEGGAPEITEIPDSEKAKAQELHNSLIESAAE
                     FDESLMEKFFEEGILSDEEIYKGLYKGVLERGIFPVICVSAANDAGVSTLMDYLVRIA
                     PSVSDSKRPLTKDGKSVDPNPDGPTSIYVFKTNVEPHIGEISLFKVMSGKLKEGDDLY
                     NVNREVKERISQIFCVAGQIRNKVEEVSAGDFGATVKLKDTRTGNTLNGKGCDYDFVG
                     VEYPEPKYRRAIKAKSEADEEKLNEILTRMHEEDPTWIVEQSKELKQTILHGQGEFHL
                     RTLKWRVENNDKIEIEFKEPKIPYRETITKKARADYRHKKQSGGAGQFGEVHLIVEPY
                     FEDVPIQDVYRFNGQEYKISVRDVQEIDLEWGGKLVFVNSIVGGAIDARFLPAILKGV
                     MDRMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFRLAGRNAFAQAFKDAGPKLLEP
                     VYDVVVMTPSDRLGDVMSDLQSRRAVIMGMNSEKGFEKLQAKIPLKELSNYSTTLSSL
                     TGGRASFVMKFASYELVPIEIQEKLLKEYEASKKEED"
     gene            complement(41560..42417)
                     /locus_tag="PLM04_01015"
     CDS             complement(41560..42417)
                     /locus_tag="PLM04_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HON01695.1"
                     /translation="MSNNLLVGKRGIIFGALNDMSIAWKVAERVVEEGATVTLSNTPI
                     AIRMGTINELAEKINAKIIPADATNLDDLEQVFSQSMEILGGKIDFILHSIGMSPNVR
                     KKRPYDDLDYDLMHTTLDISAISFHKMLQTAKKMNAINEWGSVVALSYIASHRTMYGY
                     NDMADAKAMLESIARNFGYIYGREHNVRINTISQSPTPTTAGSGVKGFDSLMDFSERM
                     SPLGNASADDCANYCVVMFSDLTRKVTMQNLFHDGGFSSMGMSYRALQQYNKSFEGSQ
                     THDGKIIYG"
     gene            complement(42635..44020)
                     /locus_tag="PLM04_01020"
     CDS             complement(42635..44020)
                     /locus_tag="PLM04_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HON01696.1"
                     /translation="MKKFIVGFFILSIFISSYGQPNDYRRWSITLDAGINNFDGDNNQ
                     NYNQIIPNSAAKASFGFSTELTLSPFWGIGTEFFYFPVSAEDAAKKFNADVFHFTPYL
                     SLNLLNLCNENNHSKWNIYATVGAGVAYYNSTLYKNGIEFDRIRDGWAAVFPVGGLIE
                     YNISSLFAIGAKVQYRLQNKDNFEGTNLKEEAGGYNFKGVTNDNIAMAALSLRWKIGA
                     KSKNHIRNNETRITLGDVFTLANRANIKTDSLAQELDKLKLSLQPGPDDDGDGVPNSR
                     DLEPNTPKGLYIDYNGRGIPEIKVIPETQEIHPEYLQYISASTKDEDKDGVPDNRDKE
                     PNSPKNLCVDFWGRSIAQDKPCAFASVYFDFDKTNLDEEALIEIEKLAEKLNTDPTLL
                     VEIRGYADNVGSNKYNKRLSQERSNRVKAELVFTHNINPQRIISNGKGKIPQPPRAYR
                     MNRRCDFILSK"
     gene            44517..44957
                     /locus_tag="PLM04_01025"
     CDS             44517..44957
                     /locus_tag="PLM04_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HON01697.1"
                     /translation="MATNRLTEQLPSNFDRYFDNEWEDYFWTDRELAANHTTLPAVNI
                     KENGEAFEIDVAAPGYEKSDIKIEMNEDLLTVSSNKKLDEEKTEGEYTKREFSYQSFL
                     RTFTLPETVDAEKISAVYENGILKITIPKKEEAKVKPARTIDIQ"
     gene            45022..45318
                     /gene="trxA"
                     /locus_tag="PLM04_01030"
     CDS             45022..45318
                     /gene="trxA"
                     /locus_tag="PLM04_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HON01698.1"
                     /translation="METFQDIINGDKPVLVDFFATWCGPCKAMAPVIESLGKEVQGQA
                     RVLKVDVDKNQALAAKYGIQAVPTFMLFKKGNVIWKHSGAIDKNTLMQQIKSAV"
     gene            complement(45395..45835)
                     /locus_tag="PLM04_01035"
     CDS             complement(45395..45835)
                     /locus_tag="PLM04_01035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HON01699.1"
                     /translation="MEENQDIKYMKQALAEARRAAEKDEVPIGAVIVCQGSIIARAHN
                     LTETLNDVTAHAEMQAITSAANYLGGKYLSDCTLYVTIEPCVMCAGALGWAQIKKIVY
                     GASDEKRGYSKFAPDALHPKTEVVSGVLAEECATLMQNFFKRKR"
     gene            46022..47152
                     /locus_tag="PLM04_01040"
     CDS             46022..47152
                     /locus_tag="PLM04_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON01700.1"
                     /translation="MKIIIVGTAYPYRGGLASFNERLAREYAREGHEVELITFTLQYP
                     NFLFPGKSQYSTEKPPEDLKIIRRINSINPFNWIKIGKEIKKKAPDLVIFCYWTSFIA
                     PCFATIARYARSPQTKMLGLVHNMIPHEATPLDKWLPGYFVKNMDGFLAMANSVKEDI
                     QHFDKKNKPKIVVPHPVYDHYGAIVSKSEAALKLGLPPKKRYVLFFGFIRHYKGLDLL
                     LEAFSDKRLKDMEVQLVVAGEFYENPEPYLKQIAQLGLANRVELRTNFIPENEVKYYF
                     SLADLVAQPYRSATQSGVSQIAYHFEKPMLVTNVGGLAEIIPNGKVGYVVNGQPKEIA
                     DAIVDFFQNNREEIFLPNIKTEKMKYGWDKMTAAINKLFFKL"
     assembly_gap    47211..47310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47324..49936
                     /gene="mutS"
                     /locus_tag="PLM04_01045"
     CDS             47324..49936
                     /gene="mutS"
                     /locus_tag="PLM04_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HON01701.1"
                     /translation="MSEPIVETPLMKQYFEMKSKHPDAILLFRCGDFYETFSTDAIEA
                     SEILGITLTRRANGSAKSVELAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLAKKI
                     VKRGITELVTPGVTYNDTILNHKENNFLASVYLNKNQAGVAFLDVSTGEFLLAEGTNE
                     YVDKLLNSFSPKEIIFDRTKKKEFENLFGTKFYSYPLEDWVFTLDAAKDRLLKQFETK
                     SLKGFGVEGLANGVVAAGAILYYLDLTHHQQTGHITQLSRIEEDRFVWLDRFTVRNLE
                     LFEAINEGGKTLLEVLDKTISPMGSRLLKRWMAFPLKNVKSINERLDVVEYFFKHPEL
                     KEALAQNIALIGDLERLVSKIAVGRINPREVVQLKTALKAIEPIKQLCTHTENITLRT
                     IADQLNACSIISEKIEREIQNDPPILINRGNIIQKGVNADLDELRELAFSGKDFLQKI
                     QERESQATGIPSLKVGFNNVFGYYLEVRNTYKEKVPETWIRKQTLVNAERYITEELKK
                     YEEKILGAEEKIIDIETRLFNELVLSLSEYIPALQLDSNLIAQLDGLLSFAKVSEENR
                     YVRPEINDTEVLEIHQGRHPVIEKQLPLGESYVANDVYLDSSKQQIMIITGPNMSGKS
                     ALLRQTALITLMAQMGCFVPVESAKIGVVDKIFTRVGASDNISLGESTFMVEMSEAAS
                     ILNNLSSRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHPAHKAKTLFATHYHELNEM
                     EKSFSRVKNYNVSVKEVDNKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRAEQIL
                     KQLETDNRQSDISKPVDKIAQHREGYQLTFFQLDDPVLEQIRDEIKNLDINNLTPIEA
                     LNKLNEIKRIIMGK"
     gene            complement(50064..50975)
                     /locus_tag="PLM04_01050"
     CDS             complement(50064..50975)
                     /locus_tag="PLM04_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HON01702.1"
                     /translation="MGNLNLKVKNLFKEQVSEWELAKKNYEDLKKVEIKVFSFGDFEI
                     VVQFNPARIVSSGAKVDEKTIKERKCFLCRQNLPPQQKCVDAEDYTVLVNPYPIFPEH
                     FTIPRKEHIPQQIKPFFPDMLELAKSLDEFVVFYNGPKCGASAPDHMHFQAGTKNFLP
                     LVNDYRRLKATHAEILEKENHYSLSFFKNYLRTVYCMESDDSQVLSFQFNRLYEDFQK
                     KMNCQANEEPMMNVVCLFENQKWIVFVFPRKAFRPWQYSAEESRQLMVSPATVEMSGI
                     FITPVEEHFRRITREDVVDILEQVSLK"
     gene            complement(51027..52139)
                     /locus_tag="PLM04_01055"
     CDS             complement(51027..52139)
                     /locus_tag="PLM04_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON01703.1"
                     /translation="MLKYLLEKEFKQIMRNKFLPRMVIILPFVALALFPLVANFDVKN
                     INLAVVDNNHSQYSERLIQKVISSGHFILTNVSHNYSDALKSVEQNKSDVILEIPAQF
                     EKDLVNNLTASVLISANTVNGMKGGLSSAYLVGIITDFSSEIREEWIQSGTKLKVPAC
                     EIIPQYRFNPHLEYKYNMIPAIMMMMLAMITGFLPALNIVGEKEKGTIEQMNVTPVNK
                     FTLILSKLIPYWVIGFIVLTICFFVAWLFYGMISKGGYLQIYLFAAVFVLAFSGLGLV
                     ISNYATTIQQGMFMMFFFVITFIFLSGLYTPISSMPEWTQTMSFFSPLRYMIEALRSI
                     YLKGSSFMDLWHQFMALVGFAIFFNFWAINSYRKKA"
     gene            complement(52157..53263)
                     /locus_tag="PLM04_01060"
     CDS             complement(52157..53263)
                     /locus_tag="PLM04_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON01704.1"
                     /translation="MKQFMSFVRKEFYHIFRDKTTMAILLILPILMLVLFGYSISTEV
                     KNSKIAILDSSNDVATRAIIEKFRNSEYFSVKKMVNNPSEIQDLFKKDRLNMVLVFGD
                     NFYENLAHTGNAQVQLIVDGSDPNSAKTIVTYATSIIGAYQLELSKIDKIPFQINPQI
                     KLLYNPQMKGAYNFVPGVMGMILMLICAMMTSISIAREKELGTMEVLLVSPMKPIYII
                     LSKTIPYFVLSLVNLATILLLSVFLLKVPIAGSLFWLIAFSLVYIFVNLSLGLLISSI
                     VDSQLVALLISGMVLMLPVIMLSGMMFPVENMPVFFQWISQIIPAKWYIIGVKKIMIK
                     GLGLSSIIPEMSVLLGMGIVLVIASLKNFKYRLE"
     gene            complement(53265..54005)
                     /locus_tag="PLM04_01065"
     CDS             complement(53265..54005)
                     /locus_tag="PLM04_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON01705.1"
                     /translation="MEKVIEVQNLTKKFGDFTAVDHISFEVYKGEIFGFLGANGAGKT
                     TAMRMLCGLLPPTSGKGLVAGFDIHTQSEEVKRNIGYMSQKFALYEDLKVWENIRLYG
                     GIYGVPEDEIIKKTDELLKRLGFEKERNSFVKSLPLGWKQKLAFSVAIFHEPKIVFLD
                     EPTGGVDPVTRRQFWEMIYEAADRGITVFVTTHYMDEAEYCDRVSIMVDGRIDALDSP
                     KNLKNQFKANNMDGVFQQLARRASRKAD"
     gene            complement(54008..54952)
                     /locus_tag="PLM04_01070"
     CDS             complement(54008..54952)
                     /locus_tag="PLM04_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON01706.1"
                     /translation="MTGNNGEFAVCCENITKTYPPDIQALKNINLKVSPHQIFGIIGP
                     DGAGKTTLFRILTTLILADEGKATVLGYDVVNEYQEIRKYVGYMPGRFSLYQDLTVEE
                     NLKFFASVFGTTVEENYDLIKNIYRQIEPFKNRRAGQLSGGMKQKLALSCALIHKPKL
                     LILDEPTTGVDPVSRREFWEMLQNLKNEGITILVSTAYMDEAGLCDTIALILNGEFLK
                     IDTPKNIVQQFEPEIFSVQSNQRSKLLTDLRRLPEVSSCYAFGDSIHVTVENKKISEE
                     ELQNFLLKNGHEQIEIAKIEPTVEDCFMELSKQQEENN"
     gene            complement(54992..>55213)
                     /locus_tag="PLM04_01075"
     CDS             complement(54992..>55213)
                     /locus_tag="PLM04_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01707.1"
                     /translation="QKAKVFVDFGKDDQKEYEGTVSWISGESEFTPKTIQTRDERANL
                     VYAVKINVKSDGLLKIGQYADVKFGAENQ"
BASE COUNT        16686 a        10832 c        10372 g        17123 t
ORIGIN      
        1 aattttcaat tcgatttttc tacctgatga tgctaatgga caatttggga taaataaaaa
       61 aggagttatt ctatttttaa ctcaatttct tcttcaactt tatcttcttc aatgaccaga
      121 ttttcgataa taaaattttg gcgttcgctg gtattttttc ccatataaaa ttcgagcaat
      181 tctgaaacag catcttctct gcgtaacgaa acctgctcta agcggatgtc tttgcctata
      241 aaatttttaa attcgtcggg cgaaatttca cccaagcctt taaagcgcgt aatttcggca
      301 gtggctccca atcgtttaac ggctgcaatt ttttcttctt ccgaataaca gtaaattttt
      361 tcttttttgt tccgaaccct gaaaagcggt gtttgtagaa tgtaaacgtg ccctttttta
      421 atcagttcgg ggaaaaactg aaggaaaaaa gtaataagca gcaagcgaat atgcatacca
      481 tctacatcgg catcggtggc aacaatgatt ttgttgtaac gaagtccttc gatgccgtct
      541 tctatgttga gagcagcttg caacaggttg aattcctcgt tttcgtacac cacttttttg
      601 gttaacccga aacagttcag tggcttgccg cgaagactga aaactgcctg cgtattcaca
      661 tcacggcttt ttgtaataga gccgcttgcg gaatcgcctt cggtaatgaa aatggacgat
      721 tcttcggcgc cgggcttcga ttcgttgtaa tgaacgcgac agtcgcgtaa ttttttgttg
      781 tgcaaactga cttttttgga acgttcacgt gccagtttcg taacgccggc tatggctttt
      841 cgttcttttt ccgaatcctg aattttttgg agcagtattt cagctgtttc aaggttgcga
      901 tgaaggtaat tgtccaattc ggttttaatg aaatcggaaa taaatttatt gacagatatt
      961 cctcccggtg caatgtcgcg cgaaccgagt ttggttttgg tttgagattc aaaaaccggt
     1021 tcttctatgt tgacggcaat ggcagccact attccgttgc gaatgtcagt atattccatg
     1081 tttttggaat aaaattcctt aatggttttt gctaccgctt cgcgaaaagc actttgatgg
     1141 gttccgcctt gcgtagtatg ttgtccgtta acaaaagaat aatactcttc tccatattgg
     1201 tcgcaatggg taaaagcaac ctcaatatct tctcctttca gttgaatgat agggtataga
     1261 gccggaacag tcatgttttc gttcagtaaa tcggtcaaac cgttacggga aataattttt
     1321 ttcccgttgt aaacaatggt aagtcccgca tttaggtaaa cgtaatttcg aagcatggtt
     1381 tccaccactt cgtcgcggta ttcgtatccg tcgaacattt cgtggtcggg tgtgaaggta
     1441 atcaaagttc cgcgttcggc atccgattca acgataccgc tgtcaaaagt aagtttgcct
     1501 ttttcaaatg cggcttttct ggtttgtcct tctctgaagc tttgcgcaat aaatttttcc
     1561 gagagcgcat ttactgcttt cgttcccacg ccatttagtc caactgattt tttaaaggct
     1621 ttggagtcgt attttccgcc ggtgttcata atcgaaaccg cttctatcat ttttcccaac
     1681 ggaatacctc gaccgtaatc gcgaacctgt accatatcgt ttacgatctg aacttcgatt
     1741 gtttttcccg ctcccatacg gaattcatca atgcaattgt ccatcacttc ctttagtagg
     1801 acataaattc catcgtcggg gtgagaaccg tctccgagtt ttcctatgta catgccggga
     1861 cggcgacgga tatgttccag cccttccagc gtaatgatat tgctgtcgcc atattctgtc
     1921 aacgtaactt tttcaactat ttcttccata tttccataga atagattata aaaaaaattc
     1981 actttaaacc aatgtgcaaa tttaattgat ttttggttta tcgtgcgagc catttttccg
     2041 gatccaacaa atttttattt ttccatattt gaaaataaag ctccgttttg ttgtcctttt
     2101 cgctgtcggt aaaaatttta cctactgctt gtttagtaga aaccttatcc cctattttga
     2161 cataaatgtc ggtcaagttt gcataaacgg tacgatattc accatgttgt ataatgacgg
     2221 cattgttgct tccaggtacg gagaaaagtt gagtaactac gccatcgaaa acagctcttg
     2281 ccgtgctatt ggcgggtgtt tgaatgtaaa tacctttgtt gttggttgta acatgtttca
     2341 atacggggtg aggctgaatg ccataatgtc cgctgataaa accgttgctg acaggccagg
     2401 gaagccgtcc cgcatttttc tcgaaattgc ccgacaataa aacttcttcc tttgttagtg
     2461 tagaaacttt gcttgttgtc gattttcctt tacttttatt aagattttcg gcttttcgaa
     2521 tttcttcggc tatcagggat tctattcgtt tgtttaaatc gttggctttt ttttgctgtg
     2581 catccaattc cttacgtaaa tttttctctt ttttctgaag atctgtcaac agtttttttt
     2641 cttttttttg ttcctgcgac agctttgttg cttctacttc tttttctttc accactacca
     2701 ccagtgtgct tttgtgtttg gatatactgt cttttttttg ttcaatctgt gctttaacct
     2761 tttctatttc gctaacttga tgcttccgat agtcgctgta ttcctttaaa tatcgaatgc
     2821 gccgtaggga ttgatcaaaa gtttctgccg aaagcacaaa cataattttg gaataaatac
     2881 tgcggttgat atatgcttcc tgtaccagcc ttgcataatc attttttaaa ttttccaatc
     2941 gacgttccaa ttgttcgatc tcttgtccca ttgtttttat ttctacatca agctgcgaaa
     3001 tttccttact cattgtttta atcagatgag tacgttcgtt gatgttttta ttgaggagag
     3061 aaagcttgtt caatgaactt ttctgccatt ttttggtttc gtccagcatt ttgctggtcg
     3121 tttccaattt ttgaagcgtt tgcttgcgtt cttcttccaa ttgttttacg ttctgggcta
     3181 agagttgcgt ccctgaacag gcaaaagcaa ttatcaataa aagctggatt agataagtac
     3241 gaaacatctt gacaaaataa aaaaaattat ataaaaaatg aatagcaaat tatgaggctg
     3301 aattgtttat tttttgaaca actgatcaaa acttcctttt ttataacgac gggtatcggt
     3361 tggtataaat tcaacttgct gattaaattt cactctatca atggtaattt ttgcgctgga
     3421 taaatgtttt tgtcctttca acatatatgt aatttgtctt ggaaaaatta tcccatttat
     3481 caaagcaaaa ttttcgtata atgcattgaa agataggtta ttaattttat cgaccagtaa
     3541 aatttctgtt atattgaaat cggggttaat gctcatggtt tgcatcagtg tttcgttatc
     3601 gaatccaaag cttatcttgt ggttattttg tgcaagaatg ctgcatgcaa tatctttctg
     3661 tcgagctaaa caaaaatagc gattggtcag cagggcttgc agatggtaaa aatcaatacc
     3721 aagcccagtc cgaagattca gatactgaaa atctgttgta taataggttt ggttaacttt
     3781 gtcgaatata aaaatattat ttggtgttat ttccagtttg aaaagctcca tgccaaaaaa
     3841 aggctgaatg gaaatattga tagcagagtc tttttttatt gtcattgaag aagttacatt
     3901 aaattcccga tctttcattt tcaggtaaat gttcattttt ttgatttctg cagtttggat
     3961 attgtccaat gcattcaact gctcgataac cttttcggta gttagctttt ttggaaaata
     4021 agagggttgc tgaacctgct tttttgtttt gcaggaactg agcaatacca atatcatcag
     4081 gaaaatactt aaaatttttt tcataaactt gtaacttttg gaagcaaaaa atttttctgc
     4141 aaattttgtt atttgttttg acgatcgaaa cctttgtttt caattttttg tttcaattct
     4201 tccgtttgat ttccgagttc gtatgatttt atccacattt ccagcgcttt ttcgtcattt
     4261 cctccataca tccagagtat gtcgccataa tgttcataaa ttatgccgtt gtcctctcca
     4321 tccttcaagt tgtcgatagc ccgttcgatg taaaattttg ccagcgaata attaccctgc
     4381 tggtacaaaa tccatgcata agtatcgaga aaagtggcat tgttgggttc tttttccact
     4441 gttttggcac tcattctttc ggcttttttc aaatctttct tttccagcga cagatagtac
     4501 gcataattat tcatcacgta gatattctcg ggattggctt tcagcgattc ttcgtaagca
     4561 atgaatgccg aatctttttg accgatttta aaataagtat ctcccagttg tgcatataca
     4621 tcgctcttca gcgctttctg ttggtctttt atataaagca gagctttttt atttttttgc
     4681 agtgaagctt gataatcatt taattgatac aaagcaattc cttgataaaa gtacaatatg
     4741 ggttcctcgg gcaatacttc gatggcgcgt tcagtagtaa catatatttt ttcaaaattt
     4801 ttgtcgctcc aatatatttg aataagtttt aaccatgttt gcaagttttt agggttgatg
     4861 tttaacatgg tttcaagctc agatatggct tccgggatac gctcctggta ttccagaaac
     4921 aaagcgtagt aggcatgcac ctgctcttcg agcggataac gatcgaccaa gagtttgaaa
     4981 agtgcttccg tttcgtcgag tttttctttg tcttttatca ggttgtcaat gtactgcccc
     5041 agaatttcta ttttggtatc cacatccaac tgttcgtttt ttaatgcgct gacaatagcc
     5101 tccgtagctt tttcgggctg attgattttc ttgtaatatt ccgaaaggga aatatagaca
     5161 aaaggatttt gtggttcttc ctttaatatt tgttcataaa tagcaaaagc ctgttcgaga
     5221 ttgtttagtt gaaggtaaat atcgcctcgc aaaaccttgt agcgattttc ggtcggatat
     5281 ttttcgatca acctgtcgat ttcggctatt gcttttttaa ctttgttttg ttgcgcataa
     5341 aggctaaatt ttgcaaagga aatgctttca tctatgccgg ttaggttttc gagccggttg
     5401 taggcatcaa tggctttgtc gatgtcgccg gaggctttgt acaactgagc aagcgtggaa
     5461 tagatttcct ctttttcagg ataaattttt tgcatctcat tggccaaagc aatagcatct
     5521 ttccaatttt tctgctgaga gtaaatgttg agcagagtca tgctgtacca ccagttggta
     5581 ggttgcaatt caaccgattt tttcaatgct ttcagggcag ggtcaatcaa cccgatattg
     5641 gcatatagta cacccagttg cgattgtact tctgcatcca gagaatcgat ttgtcggcaa
     5701 aagagaaaag tttccatcgc ttcatcgagc tgctgattat ttttgagtcg tagcgcttcg
     5761 taaaaataat agtcgaactg actttgctgt tcgggtgtca gtttattcga ccgaaaattt
     5821 ttctctcgtg taaccgattt ccttgtcgat gagcaggaaa ttgtcagaag acagagaaaa
     5881 attaatatgg gaaataaaaa aatttttttg tgcatgaaaa attgttttgc ttgttatttt
     5941 ccgataatag tagattcgat tcagaatttc tacatttttt ggaatttatc gtcccgtatg
     6001 tccaaatccg ccatttcctc gatcggtatc gttcaacatt ttgacttctt cccattctgc
     6061 atgttcatgc gaagcaatca ccatttggca tattcgttcg ccatcgttga ttacaaaatc
     6121 gtcattggaa agattgatca gaataataca aatttcgccg cgatagtcgg catcaatagt
     6181 gcccggagaa ttgagcaccg taatgccgtg tttgatggcc agtccgctgc gcgggcgtat
     6241 ttgtgcttca tatccttccg gcaactcgat aaaaagtccg gtcggaatca gttttctttc
     6301 caaaggtttt aaaactacag gctcgtcgat attggctcgc aaatccattc cggcagaaag
     6361 taaagtggcg taggatggca gtggatgttt cgaaaggttg acaattttta ttttcatcag
     6421 aatataattt attttttaaa ggtctgatgg aacccacatg tctgacatat tatattcatt
     6481 cgtgtttgtg taggcaatac atgtggattt catcagtaaa tgtttttaaa tcgaataaaa
     6541 caataccgga attttatgaa tccggataaa gcgttaatac ctcaaagata gttgattggt
     6601 atttgtttga tttgtaaccc tgttttattt ccctttttcg ctttaatatc tttaatgcag
     6661 ttaatatttc tattgtttta attttggttc cttattctct attctaaagt attttccaag
     6721 ttttttgtaa tgttcctttt atagattttt ctttcgttgt ttcctcctgc tgaaaaaatg
     6781 gttaattatt cagaaatccg taaaggctta cattttcttt ttactttttg ttacacttca
     6841 aaaaatgttc caaatactcg tgaatgcgct tttaaacaag catctgtatg cttgctatcc
     6901 aaagattgtc aaaaaggaaa atggaaagag tagagtcctt gtatgaagtc cgaaatgtaa
     6961 tcaatcgatt atcaaattca tttgcaaaat cattcattgt tactgaacag gccactattt
     7021 tttgtcagag aaattttagt atttttttgc agccaactca acaattttta caatcaccat
     7081 catagctttt tccatagatt gaatgggaat gtattcgaaa cgcccatgaa aattcatccc
     7141 tccggcaaaa atattcggac agggaagtcc catatacgaa agtcgggcgc catctgttcc
     7201 ccctcttacc ggaacaattt taggcgtaat gcccacttcc ttcatggcat cggatgccaa
     7261 atcgataatg tgaaaaaccg gttcaatttt ttcacgcatg ttgtagtatt gatctcgtaa
     7321 ttcgataaca gcggtatttt ctccaaattc tgcattgatt ttattagcca aatgctgaag
     7381 ctcttttttc cttttttcaa atttgtcgcg gtcgtggtcg cgaatgataa gatgcatggt
     7441 cgttttttca accgtacctt ctatggacgt taaatgataa aaaccttcgt aaccttcggt
     7501 atgttccggc gtttcgtggc ggggaagcat ggctacaaac tgatgagcaa tacgaatgga
     7561 atttttcatt ttatgaaatg catagcccgg atgaacattt aatccgtgaa aagttatttt
     7621 ggctgaggca gcattgaaat tctcaaattc caattctcca atttctccac catcaatagt
     7681 atatgcccaa tcggcattga atttcttcac atcgaaatta tctgctcctt ttcctatttc
     7741 ttcatcagga gtgaaagcaa tgcgaatttt tccatgttta atttccggat gacgcagaag
     7801 atattcaaca gctgaaataa tttcagcaat gcctgctttg tcatcggcac caagcaatgt
     7861 agtgccatcg gtaacaataa tatcttgacc cacatactgc aaaatttctg gaaatttttc
     7921 tgtttccagc acaattagct gttcttcgtt cagtaaaata tctttgccat cgtaattttc
     7981 aataatctga ggtttgacgt tttttccact catatcgggg ctggtatcca aatgggcaat
     8041 aaagccgata gttggaattt tcttatcggt attggccggc aaagttgcca ttacatagcc
     8101 gtttttatcc acttcggcgt ctttcaaccc aaaagacttc aattctttta ccaaatgttg
     8161 agcaaaaacc atttgacccg gcgtactggg tgtcatgttg gttaattcgt cggaagttgt
     8221 tgtaaaactt acgtacctta aaaatctgtc ggttaatgtc atgaaagtcg attttttatt
     8281 gcttcaaagt tacagatttt ttattaagaa actatttccg gataattttt tctttatgtg
     8341 cgaaaaaacg tattccgata gttgtttcaa agagagtatt tagttggatt taaattcaag
     8401 aaattaatgc aacttttttg taggaagcaa gcgggaaaga tgataaatct gttgaaaaaa
     8461 gaagtgatcc agctgggatt cgaacccagg accccatcct taaaagggat gtgctctacc
     8521 tgctgagcta ctagatcaat aataaaaatg tctgtttttt ttttaaaacg cttgcaaagg
     8581 tacaatttct tttcgaattt tcaaaaaatc aatgcaaaac tttctgctaa attcacactt
     8641 ctaagttgaa cttagcagtg aagtagaaaa cgggaaaaaa cttattgcaa tgacccgcct
     8701 atcaggtcaa ggatttcatt tgtaatgctt tcctgtctca ttttgttgta ttcaagcgaa
     8761 agttgttgta aaaggttttc ggcattttcg gtagctgctt gcatggacag ggtacgggct
     8821 gcacattccg aagcataagc ttcgagagct gccgtgtaaa tatgataatg taaagccaat
     8881 ggaattaatt gttgttcaat ttcttcgata gaaggttcaa tgagataatc gttacgaaat
     8941 tcgccttttt ttcgatagtt tatttcgatt ggcaaaaagg tttcagctat catgatttgt
     9001 gtggttttgt tcacataatg aaaatatata acctttattc gatcgatttt gtgttggaga
     9061 aaaagctgga taagagtatc ggctatctga gtggtgtttt cgatatcggg atgagcagcc
     9121 acattttgaa attccccagc aatggccaaa tttttttctt tgcaaaaatc ctccactttt
     9181 ttgccgatcg gaaaaattag gatatcttcc tttcttacgt cacgatgggt ggtaatagat
     9241 tgttccagtc ttttgattat gtttgaatta tagttaccgc agagagaaga attggaagaa
     9301 aataccacaa tgccaacatg attcacatgc ctgatttctc tgaaaggcga tctgtcaaca
     9361 atttggctac ttaaataatt tctcaatata tccgttaagg tttcccgata agtcaagaaa
     9421 ttgtaaacgg ctttttctgc ctttaaaagt tttactgaag agaccatctt catggctgaa
     9481 gtaatttttt gagtagattt taccgattgt attcttgatt taagttcctt taatgaagac
     9541 atgatgtgtg aaattgattt tttaaaataa agcttgttgg gagatggctt taataattca
     9601 tttgtttttt taatatttct gctgcctctt caatggaaga agtaactttt tcgtcaaaat
     9661 ttcctttttt tatttcattc agaatttttt ccttgtaaga ataatccaaa agattaatga
     9721 aattttcttc aaattcttta accttatgga gtggtatatt tgccagcaat ccatgtgttc
     9781 cacaatagag tagcgctacc tgtttttcca cagataacgg ccgatgtagg gactgaataa
     9841 gcaattgggt atttttttgc cccttatcta tggtcatagc cgtcaccgca tccatttcac
     9901 ttccaaattt agtaaaagct tccagctcgc gaaattgtgc ctgatcggtt tttaatgtgc
     9961 cggctacttt cttcatgggt ttaatttgtg cattccctcc tacacgagaa acggaaattc
    10021 ctacatcaat agacggtcta ttcccttcgt tgaacaaatc ggtatcgaga aaaatctggc
    10081 catccgtaat agaaataacg tttgtgggaa tatacgccga aatatcgccg gcttgcgttt
    10141 ctataatggg caatgccgtg agcgaaccac ccggtttcac tttcccttta aggcattcag
    10201 gcaagtcatt catttgtttg gcaacttcct gctgattaat gatttttgca gccctttcca
    10261 gtaatcggga atgcagataa aagatatcgc caggataagc ttctctaccg ggaggacgtc
    10321 gaagaatcaa cgagagttct cgataagcga tagcttgttt ggacaagtca tcgtaaacaa
    10381 ccagtgcatg tcttcctgta tcgcgaaaat attcgccgat ggcagctccg gcataaggtg
    10441 acagataccg caaagcagca gaatcggctg ctgtagccac caccagaatg gtgtaaggta
    10501 ttgctccaaa atcttttagg gtgttgaaga gggttgctac agtagaacct ttctgccctg
    10561 tggctacgta aatgcagtaa acgggatctc ctgctttaaa attgtccaat tgattgatga
    10621 tggtatcgac cgcaatgctt gttttccctg tttgacggtc gccaattatt aattcacgtt
    10681 gtccacgtcc aataggaatc attgaatcaa tggccttcaa acctgtttgt aatggttctt
    10741 ccacaggctg tcgaaaaatt actcccggtg cttttcgttc caaaggcatt tcgaagaatt
    10801 ctccatctat tttacctttg ccatcaatag gttcacccaa aggattgata acacgcccca
    10861 acattttttc gctaactttg atggaggcaa ctctgccggt tctttttaca gtgaaacctt
    10921 cttttatcaa gtgggtggga ccaagcaaca cagctccaac attatcttct tccaaattca
    10981 tggcaatggc ttttactcca ttttcaaatt caagcaattc attggcttct gcatttgtca
    11041 atccatatat tctgacaact ccatccccta cttcaagtac ttttccggtt tcttcataat
    11101 aactctcgat gtcgatttgt tggagttgtt tttttatcag ctccgatatt tcacttacct
    11161 taatagtctt attatcagac atattcttat aattatattt ttaggtttac gaaaagaatt
    11221 ttttcaggtt aaaagaatat tttcgttcaa ttctttttga atttttttta gttgcgttgc
    11281 caagcttccg tcccatcttc ggtttccaat ttcgatgata aaacctccaa taatttcagg
    11341 agcaattatt ttttccacct ttaattcttt cttaatataa gttttgattt gagaaatcaa
    11401 ttttttttct gtttcggcat ccataggaga agctgtagtc agttttacca atgccaagtt
    11461 tttgttttcg taataataat cggaatattt taaacctata tttcgcaaaa gatattcacg
    11521 acggttttgc aacaatacat ccataaaacg ctctaaaatg aaatcagatt tgttaccaca
    11581 ggcaaattgg ataagttgct tttttgtttc ccttgtcaac aaaggatttt ttaatgcctg
    11641 cgctagttct gcattggcag aaaaagattt ttcaagtgtc tgaaagttgg caaaaacaat
    11701 ttcctgcgca ttcatttcag aagcgaattc atacagggct tttgcataac gtgaagctat
    11761 caaaccggtt tgcatgcata taattgttac aatagataat aaaattttta aagtggagtt
    11821 ttattgctgt tcggaataaa taaatttttc aatttccaaa tttaccgata tccatctttt
    11881 ccaacatccg attgataatt tccatttggg cgggcttatc atttaatttc gattgtaaca
    11941 gcttttccga taccgcaatt gaaatctcag caacctgttg ttttatctcc tgtaaagcca
    12001 tcattttttc atacctgatt tgtttttttg cttcttccat gattttttca gcttctataa
    12061 aggctttttc ctttgcttct tttattaaag catttttaat ttcagtagtt tcatcaagta
    12121 tttttttccg gtttaaaaaa gcttcatcct gtattttttt tgcttctgtt ttaatttctt
    12181 cgagttcttt gcgtgcattt tcggcttcca acaaagattc ttcgatgtaa gaccttcgtt
    12241 tttctatcat ttgaagaatg gcagggaaac caaatttaac aagaataaaa acaactattc
    12301 cgaatgaaag catcatccag aaaaataaac caaaatcggg cattagaaga gacatagcat
    12361 ttttttaatg attaagcaca caattaatag aatcaaatac ttttgatttt tccaatatta
    12421 tcaatgaata accctcctga gacaagcctt gaaaaatttt tttgattaaa gaataaatcc
    12481 acaaactaca atggcaaaca aagcaactcc ttcaataaga gccgcagcga taatcatgtt
    12541 cattcgaata tccgaactca tttcaggctg acgcgcaata ctttccatag ccgatccgcc
    12601 gattttcccg ataccaatac ccgctgctat agctgcaaga cctgccccca atgcggctcc
    12661 aaattttgcc aatgcatcac ttgcggcaac ttgtaataaa atagatagta acatcataac
    12721 ttcttgtttt taaatgaaat ttttataatg atattgaatt aattaaagtc taatattagt
    12781 ttttcgcatt ttcaaattgt tcaaacttgt ttaactactt cctttgctgc ctgtttatgg
    12841 gaaggctcta ctcttgccaa tccgataaac acagccgaaa gcatggtaaa aatataagcc
    12901 tgaatgtatg ctatcagcaa ttctatcagg tcaatgaaaa ccgtaaaaat cactgaaaga
    12961 gccgacatgg ttacatttat ggttgttccc aattttaccg taacgaacac cagacaaacc
    13021 aatccgccaa ctatagaatg ccctcccaca atattggcaa agagacgaat cattaaagca
    13081 aagggtttgg taaaaattcc aataaactct acagccggca tcaaaggaac aggaactttc
    13141 agccaagcag gtacttcagg ccaaaaaatt tctttccaat attcctttgt tcctgaaaag
    13201 tttacaatta acattgtaaa aagagccagc gtaaatgtaa tggcaatgtt tcctgttaca
    13261 gtggctccaa aggggaaaaa cggaatcaat cccatcaagt tattgagaaa aataaagaaa
    13321 aaaaccgtca gcaaataggg cgcataccgt cggtaatcat tcccgatact tggcttgata
    13381 acaccttcgt aaatgtcaac aatcagcatt tccatcagga taacaaatcc ttttttgggg
    13441 aaaattccat cttttttgta ataatgagct atactcatga ttaaaataat cagcaaaacg
    13501 gaattaaaaa taagcgcagt agccgttttt gttaaggaaa tatccaaagg ccttatttct
    13561 ttaccatttt catcgagttc cacaatttta tccttgtatt ttccttcttt tgcaatatag
    13621 aaacccttgt aaacaggttg ttcttcaaat ttggaagaca taaaaacatg ccatcccgaa
    13681 ttattgctgt gaagaatgac aggcaaagga atcgaaattg atttgctttt ccaggtagta
    13741 atataccatt cataggaatc actcacttct gtaagaatga tttcccgaac atcaatttct
    13801 tttcttttcc cctcataatt attgttctgg gcaagtattt taaaagggga aaaagcaaga
    13861 aaacaaataa tatagacaaa acagcatttc atttactttg ccgaacttga cgtttttcgt
    13921 tcatataaaa acaagcagtc tcaaaaatca gataaattag ataataagca ccataaagga
    13981 gaccaaacat ccttcgttct gttctaaaaa ccaacaaata aacaaaagct acgattcccc
    14041 ataaaatcat ttttgaaagc tttaacagta aaaacagata aattttttta gtagatgaaa
    14101 tataagaaaa tttttgtagc acaagcatta aagtcaatcc agaaaaataa aacaataaag
    14161 gcaacaaaaa ataattttcg atagtcgtgg aagaaaattt tttcaagagc aaccagaaaa
    14221 atagccctaa caacaatgtt agtaaagaaa aaaaaactaa tgaaaaaaga agattatttt
    14281 tcattttctg gaatttgttc agcacaaata acgaccttat cgttattcaa ttcgattagt
    14341 ccgccattaa taaaaacatt gttaacaccg tttttgaaac gaaaagtaac atttcctttc
    14401 cccaaaatgg caatcaaagg agcgtgatga gaaagtatgg taaatacccc ccccttaccc
    14461 ggtactgtta cagatgaaat ttcaccatcg taaatcaatt tattgggcaa aacaattttc
    14521 agattcattg atcctcaatt ttaaatgttt tattcaaatt gtgagcttct cttagcaatg
    14581 catgcccctt atttaagaca tccttaatgg taccaacatt aagaaaagcc atttcgggaa
    14641 ggtcatcgag cataccgtcc aatataagtt taaagccgtg aatggtttct tctataggta
    14701 ccattactcc cggaactcct gtaaaatgtt ctgccataaa gaaaggctgc gaaagaaaac
    14761 gttgtacttt tcgagctctt tgcactgtta aacgatcttc ttcagagagt tcttctatac
    14821 ccagaatggc gataatatct tgcaactcct tgtttcgttg aagaatttgc ttcacccgct
    14881 gagctgtttc ataatgttcc tgcccgacaa taagcggatc gagaattcgt gaagtagaat
    14941 ccagtggatc gacagcagga aaaattccca atgaagcaat ttgccgactg agaaccgttg
    15001 tagcatccaa atgtgaaaaa gtagttgccg gagcaggatc ggttaaatcg tccgctggaa
    15061 cataaatagc ttgaacagaa gtaattgacc cacttttagt ggaagtaatg cgttcctgaa
    15121 gaagccccat ttcagtagca agtgtaggtt gataaccaac agcagaaggc aatcgaccca
    15181 acagagccga aacttcagag cctgcctgag tgaaacggaa aatattatca ataaacaata
    15241 aaatatcatg attctcactt tcatcactgt cgcgaaaaga ttcagctacc gtaagccccg
    15301 acaaggcaac ggaatatcgt gctccgggag gttcgttcat ttgtccaaag accaaagtcg
    15361 cctgagattt ttttagttgc tcataatcaa ctttcgataa atcccatttc ccttcttgca
    15421 ttgctttgtt aaattcttca ccatagcgaa taactcctgc ctctatcatt tcacgcaaca
    15481 ggtcattccc ctcacgggtt cgttcaccaa cgccggcaaa aaccgaaaat cctttgtgcc
    15541 atttggctat attgttaata agctccataa tgataaccgt tttgccaaca ccggcgccgc
    15601 caaaaagtcc aatttttcca cctttaacgt aaggagcaag taaatcgaca actttaattc
    15661 cggtatataa aatttcgtcg ttgtttgcaa gttcatcaaa tttggggggt tctttgtgta
    15721 tgggagccga ctctaccctt cccagtgggc ttaagccatc tatcgattcc cctatgacat
    15781 tcatcaatct tccctttact tgattcccta ctggtatgga aattggcgaa cccaaatcgc
    15841 ttacgggcag acctcgactt aacccatcag tggaatccag cgcaattgtt cggacaatat
    15901 tctcgccaat atgttgttga acttctaaaa tgagattttt cccgttggaa agcgaaatac
    15961 tcaaagcgtt atttatagga ggcaattcaa attcattttt ctcttcgaga gggaaaaaaa
    16021 catccactac cggaccaatt atctgccaca cctttccctg tattgataac atgaaaaaat
    16081 tataattgta aatacatttt gtgagaaatt gcgtggcaaa agtaaataaa agattcttct
    16141 taaaagaaaa cgaatgttta aattagttca aaaaaacaaa tctttctgtt aaacaatatt
    16201 attcagttgt tcaatccaac ttcaaaacag ctaaaaaggc cttttgtggt acttcaaccg
    16261 atccgatttg cttcattcgt tttttccctt ttttctgttt ttccagcagt ttacgctttc
    16321 gtgaaatatc gccgccataa catttggcag taacatcttt tctgacgggt ttaatagttt
    16381 ctcgtgcaat aattttggcc ccgatagctg cctggatggc aatttcaaat tgctgacggg
    16441 gaatcagttc cttcaatttt tcgcacattc gccgccccaa tgaataggca ttatcggcat
    16501 aaatcaatga agaaagtgcg tcgacaggct ctccgttgag caaaatatcc agctttacca
    16561 gtttggaagc tctgtagcca ttcatgtgat agtcgaaaga agcataacct tttgaaatgc
    16621 ttttcaactt gtcgtaaaaa tcaaaaacta tttcgcttag cggcaggtcg taaatcaatt
    16681 caatccgatt gcccgaaatg tattcctgct tgatcagttc tccacgctta cccagacata
    16741 aagtcataat gggaccgata tattccatcg gagtgataat ggaagctctg atataaggct
    16801 cctcaatgcg atctatcaaa gtcggatcgg gcatttcgga aggattatgc acctcaataa
    16861 gttctccctt tttggtataa attttatagg aaacatttgg aacggtggta ataacattaa
    16921 tgttgaattc acgttccaga cgttcctgaa taatttccat gtgcaacatt cccaaaaatc
    16981 cgcaacggaa tccgaatcct aaagctgtgg aagattccgg ctcgaaagtg agcgaagcat
    17041 cgtttaactg caatttttct atggaagccc gtaagtcttc aaaatcctct gtatcaatcg
    17101 gataaacacc ggcaaatacc ataggcttaa cttcttcaaa accggcaatt gctttttgac
    17161 agggacgttt cacatgtgtt atcgtatcac ctacctttac ttctttggat gttttgattc
    17221 cggaaataat atagcccaca tttccggcgc tcaaagtttg tacaggttga ggttcgagct
    17281 tcagcactcc aatttcttcg gcataataat ctttgccagt agcaacaaat tttacccaat
    17341 ctcctttgtt gagtgttcca tttacaattt taaagtaggc aataattccc ctgaaaggat
    17401 taaaaatgga atcgaaaatc aaacattgca atggcgcttc agggtctcct tgtggtacag
    17461 ggatgcgttc tacaatagca ttaagaattt cttcgactcc ttgccccgtt tttgcactag
    17521 ctcgaataat ttcttccctg ctacaaccaa gcaattctac tatttcatct tccacttctt
    17581 ccggtcttgc gctgtccata tctattttgt tgatcaccgg aataatggtc aaattatgat
    17641 caagagccat gtagaggttc gaaatagttt gcgcctgaat gccctgcgtt gcatccacta
    17701 ctagcaaagc tccttccgaa gcagctatgg aacgcgaaac ttcatacgaa aaatctacat
    17761 gtcccggagt atcaattaaa ttcagaatat aatcaatgct ttgatactga taattcattt
    17821 ggatagcatg gctttttata gtgatgccac gttcacgttc cagctccata ttgtcgagca
    17881 cctgttcctg caagtctttt cccacaaccg ttttggtata ttccaataaa cggtcggcaa
    17941 gcgtactttt cccatgatca atatgagcaa taatacaaaa gtttcggatg ttgttcattc
    18001 gattttcttc aaattttaag aactacaaaa atacatgaaa attcaaaatt aacatacaaa
    18061 cggataaaaa tagatttgta gaattgtttg atttgtatta gaagaaatag attaatctaa
    18121 tgatataaat caaattatcg aatggcgtac attcttataa taaattgaaa atcaatgctt
    18181 tagttatatt aaaccttttt aattaacgtt tcgataaatc gttgtaaagc atttgaagat
    18241 aggcttcacc ttttttcttg ttggcattcg gttgtcccca attccaaagc actttgtttg
    18301 tactgcagtc aaagacgtaa tgattttcag gggttaaaaa accgaaacca ttagggaacg
    18361 tgtaaaaagc aaaatgggga gaagctggat tcaggatatt tttgctgaaa gtaaaatctt
    18421 gatgcggtaa ttcaagttga taaagtaggg ttgcagcaat atctatttgg gacgcatacg
    18481 tatctatttt cattggcgat tttacagcac caccaataat gagcagtgga atttgatacc
    18541 tttcaaccga agaaagtaga atattttttg gataatgcat ggcatggtcc ggcacaagaa
    18601 ccaaaacagt attttcccag tatttggttt ttctgaattc gtcaacaaat gcgcccaaac
    18661 aactatcggt atattgaacc gaatttaaat agggatcgct gtttttataa aaggggacat
    18721 cgaaaggctc gtggctgctg agtgtctgaa gaattttcat aaagggttcc ttttgttctt
    18781 ttttcaaatc attggttaaa cgctgaaaaa cgatgtgatc gtgcgcaccc catttcgttg
    18841 ttctgtcttt gagcggaaaa tctttgtcaa caaccatttt gtcgatgcct tgagcaatca
    18901 ggtaagctct catgttggtg aagtccacat cgcctccata gtagtattgc aaatcgtaac
    18961 ctgcagtttt caaagttttg ggaatggaag gcaaagactg gcttttcttg ggatatttca
    19021 taatggaagt tgtgggctga gccggatagc cgctgaaaat ggaaatcagt ccgcgatcgg
    19081 tacgaaaact gtttgcgtaa aagttggtaa acaggatgcc ttcatcgcaa aattcattca
    19141 tgcgtaccgc aattccggga aaacctccca gcggctcaag gttgttggac atgaaacttt
    19201 ccaccactac aaaaataaca tttggtttcg gactggtaaa taatttaggt agttcttcct
    19261 gtaccggctg gtcgattaaa ttattaaatt ctttttgagc ctcttcttca gacatgaagc
    19321 ggtattgttt gtcaaaattt tgtgttttga atgccgattc caaaaaattg aacatgggat
    19381 taatggcagc atgattcaac tcatttttcg ggctgaaata aaccttgctc acattcatgg
    19441 tggaaacgtt aaatccgcct ctgatgggaa taaacagcag gcaggtaaac aaaaagagca
    19501 agcaggctgt agaaagtttg tttttgacct gtttggattc tttgttgatt ttgataagca
    19561 aaaagtaaaa aattgcgtac aaggttaaag caatcagaat gatagccaat agcccgacaa
    19621 caatcatcca aatatcgatg cttgccaatg cttctttcgg ccatttcatg taaaacagta
    19681 cagtggaatc gagacgaaaa ccccaaaatg gatacagtgc agcgtcaacc acaaaaacaa
    19741 cggaaatgat aaacgaaata agaccaaaat atattttttg aaagtattcg tgaaccgaag
    19801 gttttgccca taacgaaaca attaagaaaa ttcccggtaa aatagtgaga taagccgaca
    19861 tggacgcatc cagctttatt ccgtgtagaa taattagtat taaatcctga aaacttagat
    19921 tactgtaaat ttgatgatta taaagaatga aaaatggctt ttgaagcaca aaaacaaaaa
    19981 gtatcaacaa ataaatttta aaaaattgaa ttattttttg caacattaga agttatttga
    20041 aaaagttgga aatttggaaa aaaatacttt caagcctgat tttaagaaaa atcggacaaa
    20101 gttaaacgat tcctttcaaa agaatagatt ttctgaagat ttttttggaa ttatatttta
    20161 aactcgataa tatatgaaac ccacatgtat tacttacaca aacacgaatg aatataatat
    20221 tcagacatgt gggttccatc agacctttaa aaaataaatt atattctgat gaaaattgtc
    20281 aaaaaacttt tttaaatatt tttcttcctt tttatctcaa tgtcttaaaa aaaccaattc
    20341 tctttgagta acttttcaga ggagactctt cctttaaatc agttatgagc cgagtataaa
    20401 gactttttta ttcgtaataa aaattaccaa tttaaaatgc gataaaaatt atttcaatat
    20461 ggctgtcaat atgtcaccgg aaaaagcatt ggcatttttt ttgcaaaaaa tcggcgggaa
    20521 aagttgggaa tcagagattt tttcgctttt ccaacgcata aaaggaattt aaaaacttat
    20581 taaatccaaa ataaacatga caaaagggac tataggggtt acgagtgaaa atattttccc
    20641 cgtgattaag aaatttatgt acagcgatca tgaaattttt ctgcgcgaat tggtttccaa
    20701 tgctgtagat gctacccaaa agcttaaaac tttggcttca gtgggcgaat tcaaaggaga
    20761 attgggagat ttgaaaatcc gcatttctgt ggacaaaaag aaaaaaacca tcaccatcag
    20821 cgaccgcgga ataggcatga ccgccgaaga aattgaccag tttatcaatc aaattgcttt
    20881 ttccagtgcc gagcaatttc tcgaaaagta caagaacgat gcacaatcca ttattggtca
    20941 tttcggactt gggttctact ctgcatttat ggtttcaaaa aaagtggaaa tattcaccct
    21001 gtcgtacaag gaaggtgcgg ttgcccaaca ctggtcgtgc aacggagacc cggaatatgt
    21061 acttgaagat accattaaat cggacagagg aacggatgtc gttttacatt tggatgacga
    21121 taatcttgaa tttttggaag aatcgaaaat tcgtgaacta cttttgaaat actgtcgttt
    21181 tctccctgtc gaaattgcct ttggaaaaaa gaaggaatgg aaagacggca aatatgtgga
    21241 aactgatgaa gatgagatta tcaacaatac gcagcctgca tggattcgca aaccgagtga
    21301 actgacggaa gaagattatc tgaatttcta ccgtgaattg tttccctata acgaagatcc
    21361 gctgttttat attcatttga atgtggatta tcctttccac ctgaccggaa tcctgtattt
    21421 ccctaaaata aaaaacaata tcgacataca gcgaaacaaa attcaacttt actgcaatca
    21481 ggtatttgta accgatcatg ttgaaaatat cgttccagaa tatttaactc tgctgcatgg
    21541 cgttatcgat tctccggata ttcctttgaa tgtatccaga agttttttgc aaaacgactc
    21601 gaatgttcgc aaaatttcca accatattac caaaaaagtg gccgatcgct tgcaggaaat
    21661 ctttaagaat gacagaaatc agttcgaaca gaaatgggac gacctgaaac tgtttattca
    21721 gtatggcatg ttgaccgacg aaaaatttta tgaacgagcc gaaaaatttg ccttgttcaa
    21781 aaatgttgat ggcaaatatt tcacttacga agaatacaaa accctgattg gcgaaaatca
    21841 gaaagacaaa aatggcgatt tgatttatct gtatgcaacc aacgccgaag aacaatacag
    21901 ttacatacaa cacgccaaag acaaaggcta tgatgttctg cttatggatg gtcaactgga
    21961 tattcatctc ataggtcatt tggagcagaa atgggaaaaa acgcgttttg tacgggttga
    22021 cagtgatgtg atagaaaaac tcattcagaa agaggatacc gccaacgttt cattaagcga
    22081 agcacaacgc gaagcattga ctgcaatttt caattctcag ttgcctccaa tggaaaaaac
    22141 aaactttatc attacttttg aacaactggg acccaatgta aatccggtac tgataactca
    22201 aaacgaattc atgcgtcgaa tgaaggaaat gtcggccatg caaggcggaa tgatgaattt
    22261 ctacggagaa atgcccgata gctataatct ggtggtaaat actacccatc cgttgatcga
    22321 aaaattgctg aaggatgaag aaacggcttg tgccgaagaa ttggcaccga ttgaagccga
    22381 acttttggaa ttgagcaaca aactccaaca actgaaagag aatcataaaa acaaaaaaga
    22441 ggaagaaatt ccggcagaag aaaaagagga gttgacaacg ttggaaaaaa ggattgatga
    22501 actgaaagaa aaaagaaaat cggttcttgc agcctttgca agcaactata aaacagtgcg
    22561 tcagttgatc gatctggcac ttctgggcaa caacatgttg aaaggagaag ctcttgccaa
    22621 tttcgtgaaa cgaagcatag atttaatgta aaaatttctt tctgctttac ttgttgaagc
    22681 tcgtcggatt ttcctaaact gacgggcttt ttttgtgcag atattttgaa catgcagttt
    22741 tttgtattac ttttgcaact tcattttatg taactaactg attactaaaa taatgagaaa
    22801 gaaaacgact ttatttttcg cctttttacc gctggctgct atggctcaga attttcaact
    22861 gcattacgat tttggacagg ggcgacatta cgttacaacg actttcgaaa tgttcaaacc
    22921 cgatgattgg ggaaacactt tcttttttgt ggattacgat ttcaacatgg atcaagatca
    22981 taatgcaagc ctttcttata tggaattggc acgatgtttt tcattgggta aaacgtctcc
    23041 tttcagtgtt caggtggagt acaacggcgg tttgtttgct atggaaggat ctgcttatcc
    23101 cattcaaaac gcttttctga tcggactcga ctatggctgg cacaacaaca atttcgatcg
    23161 ttttctgaat tttaaagtgc tttacaaaaa tattgttggc aaacatcctc tctctttcca
    23221 acttacggga gtttgggatt taagctttta caataaaaga atttccgttt gtggtttcgc
    23281 cgacttttgg tgggaagata atctcaattt cacagatgcc gccggtaaaa atctcgattc
    23341 accggtgctt actcggtacg tctttatttc cgaaccacaa ttctggtaca acattacccc
    23401 acatctttct gccggttcgg aaatagaaat agcagccaac ttttcctccg tttatggatt
    23461 aaaaatttgt cctacattgg gaatgaaatg gaatttctaa aatgaatttc ccctgaaaca
    23521 ttttagaaaa aagcataccg aaaaattcat ttcaattcat tatctttgct gattgtaatc
    23581 tcattttaaa tagaaaatga aactgttgaa caaaaatgag cagcaaattt ttatcgcaga
    23641 aaaacagcat tcagtgaaaa attttttggt atttattgta atggccggat ttttggtgtc
    23701 ctgtcattcg gcaacccaaa aatctgctaa tgaagaaaaa gtcataaata ttcctgtgtt
    23761 tgactcagat tcggcttttt cgtttgttca gcgtcaggtc gattttggac cccgtgttcc
    23821 aaatacgccc gcacacgatg cttgtgccaa tttttttgtt gcaacactga aaagttttgg
    23881 ggcacaagtt acaactcaac aaatggactt gcggactttt gatggaactt tgcttcatgc
    23941 tgtgaacatt attgcttcct atctaccgga atcggaaaca aggattttgc tttttgcaca
    24001 ctgggactcg cgcccctggg ccgacaacga cccaaatcca aaaaatcggg aaaaacccgt
    24061 tttgggagca aatgatgggg caagcggaac gggagtttta ctcgaaatag ctcgacaatt
    24121 ggggaaaaac ccggcaaatg tgggagtcga tataattttt tttgatgccg aagactgggg
    24181 agcatccaat gatgaccaaa cttccaatac ggaaaactcg tggtgcttgg gcgctcagta
    24241 ttgggcaaaa catcctcaca agcaaggtta tcatgcacga tacggcattt tgctggatat
    24301 ggttggagca ccacaggcaa ctttttatcg ggaacaatat tcggaatatt atgctcctca
    24361 tgttgtggaa aaagtctggt ctcaagcgca aaatctcggt tttggtcagt attttatcaa
    24421 ccagaaaggt ggagtaatca tggatgatca tgtttttgtg aatcaaatgg caggaattcc
    24481 gagcatcaat attatccatt ttgatcccaa tacttcctct gggtttggga attactggca
    24541 tactacgcat gacgacatga gtaatattga taaaaatacg ctttttgcgg taggaactac
    24601 cttaatgaat gtaatttaca acgaaaaata atgtatggaa tttcgtttta aaagaattgc
    24661 gataaaaatt ggaagtaatg tccttaccaa atctgatggt acgctcgata cagaaagcat
    24721 gtcgaatttg acgaatcaga ttgccacact gtatcatcaa ggaatcgaaa tagttgtaat
    24781 ttcttccgga gcagttgctt cgggtcgaag catcctggga ctgaacaaaa aaatggatat
    24841 cgttgaccag cgtcaattat attccactgt aggccaggca aaactcatca gcttgtattg
    24901 ggatttgttt gccaagcacc atattaccat tggacaagtg cttaccacca aacagaattt
    24961 cgacagccgc agaaattatc tgaatcaacg caactgcatg cgcgtaatgc tcgagaacaa
    25021 ggttttacct attgtaaacg aaaacgatgc agtttctgtt tctgaattga tgtttaccga
    25081 taacgacgaa ctttcgggtt taatagcctc catgatgggg atggaagcac ttttcattct
    25141 cagcaacgtg gatggcattt atcgtggttc tccttccgac ccaaaagcag aagttattcg
    25201 ggaaataaag agagggcagg atatttccga ttttattcag accaccaaat caagtttcgg
    25261 acgtggagga atgcagacca aagtttccat tgcccgaaaa atagccgatc agggcattga
    25321 agtatttatt gccaacggca gaaaggaaaa cattttggtt cgcttgatag atgccgctga
    25381 cgatgtggtt tgcacacatt ttatcggata cagaggcgaa gtatcgagcg taaaaaaatg
    25441 gattgctcac agcgaagatt ttgccaaagg cgaaataaaa ataaatacca atgcagtaaa
    25501 agcgctgatg ggcgataaag ccgtgagttt gctgcctatc ggtgtaacgg aaattattgg
    25561 agatttcgaa aaagacgata ttgtgaaaat tgttgatgaa aacgggcagt cgataggtgt
    25621 cggaaaagtt cgctacgaca gcaaaaaggc aagagaatta ataggaaaaa agtatcagaa
    25681 acctttggta cactatgatt atctttatct ggaataaatt gatgaaaaaa gtttctaaaa
    25741 atgttgatta tttgacatcc agtttattaa attaattttt tacccgtatc tcattcattt
    25801 tttgcatgag ttacaatttt gcgcacagat tttgaatggt gaaggattaa attggttttg
    25861 tgagagaaaa aagcaaggca gcgtctatgc gaaacataaa aataagactt aaaattgaaa
    25921 cgattgcagt ggctttactg ctaacgggat gtaacacaac aaaattcgtt ccggaaggag
    25981 aatatttgtt ggacaaagtg aacataaaca tagacacgcg gaatgtcaaa aaaatcgaat
    26041 taaaagaata tttgcgtcaa acacccaatt cttcggtttt tggtcttttc cgtacccaac
    26101 ttggcattta cagtcttgca ggaaaagaca cttcaaagtg gatcaacaaa acactcatgc
    26161 gcattggaga agaaccggta atttataatc ctgccctgac ggaaatttcc tcacagcaac
    26221 ttcagctttt catgcacaat aaaggttttt tgagagcgaa agtggaaaac aaagttacac
    26281 ttaaggataa aaaagccgaa gttgaatacc aaattcttgc tcatcaaccc taccgtatcc
    26341 ggaattattc ggtacagttg aaccatcccg aactgcaagc tattgcagcc gacacttccc
    26401 gctcgctcat tcggccggga atgctttttg acgtggatat gctcgatgcc gaacgggaaa
    26461 gaatcacttc tcgttttcgc caaacaggct attacaattt cagtaaggat tttctggttt
    26521 attcggccga cagcatagaa aaaacgcatc aggtggattt gtccatgcaa ttgagagatt
    26581 atctgaacga caaaaacgac tcactttccg aaatcatatt cagaaaattt tatgtgagaa
    26641 acgtgtattt cgaaattaac cccactgacc ttccatcact gaatcaacct atggaaaagg
    26701 atacggtgtg gttccgaaat tttgccatga tcactccaca caagcaaatt attaaaattg
    26761 acgcattggt tcagaacaca tatattgatc cacaagcttt atttaacgat gccaacatag
    26821 aaaaaactta ttcagggctt aattcgctgg gtgccataaa gtttgtcaat atcggttttg
    26881 aacaggtcga tacgggtcta ctcgattgcc acatcagcat tattccgggg aaagccattt
    26941 ctctttctgc tgaagtcgaa gccacttata cggatggata ctggggtgga gcaaccaatt
    27001 tgaaccttgt caatcgaaat atttttaaag gtgccgaaac tttatccctg caggttcatg
    27061 gagcttatga acttcaggaa aaggtgtggg cacaggaatg gggagggcag attgggctgc
    27121 aatttcctcg tgtggtttta ccttttgctt cttatgattt caagagaaac cttcgggcta
    27181 acacggaatt ttccggttca tactattatc agtttcgacc gggcgaattt cgaacaagca
    27241 acttgggtgc cggcatgaga tattttctca acagaagaca atatacccat atttttgatt
    27301 tgtttgaagt caactatgta tattttcccg aaattttccc tgctttccgc gacagttttc
    27361 tcacaaccgg catttttaat tcttacaatt acgaaaatca ctttatcatg cgaatgggtt
    27421 attcaggctc ttccacaaac tttaacgcca atcaaccgtt acggaactac cgtaccatgc
    27481 gttacagcat agaaacggcc ggaaatattc tatacggact ggatcatttg ctgggcagcc
    27541 gtccggcgga agatggaaat taccgcctgc tcaacattgc gtatgctcag tatttcaaaa
    27601 cagaatataa tctaacttat catcaaattt tcgatgaaaa caatcgtttt gtttaccatg
    27661 ccggattagg aatagctatt ccttacggaa atgccgatgc agttccgtac gaaaaacgat
    27721 ttttcagcgg aggagccaat ggcgtcagag gttggagtga aagcacattg ggcccggggg
    27781 tttataaaag aatcaatacc caacgcagag atttcaatca gaccggcgac atcaaactcg
    27841 acatgaatat ggaatatcgc ggaaaattgt tttggttgct cgaaagtgcc gtttttatgg
    27901 atgcaggaaa tatatggact atcaaggatt atgatacgca acagggtgga ttgttcaaat
    27961 ggaattcctt tatgaatcaa attgcagttg cttacggagt cggactaaga ttcgattttt
    28021 cctttatcgt tgcccgtctg gatatgggag taaaacttta cgacccggta ctgaaacgca
    28081 gcgaccgatg gagggtttcg ccgcaatgga acgacgtggc tttccacatt gccattggtt
    28141 atcctttctg acgagcattt ttccggttgc agttctgttt ggttaaaata gttcctcctt
    28201 cagcagacga aaacttaacc ggtgatactc cgtgcagcca taccgacgaa tcatttcacg
    28261 atgccttgct gtaggatatc ccttgttttt cttccattca tattcaggaa atttctcccc
    28321 gagtatttcc atcagttcat ctctgtgcgt tttggccaat accgatgctg ctgcaatgga
    28381 aagatacttg ctgtctcctt ttacaatggt ttgaaacgga atatcggcat aaggcttaaa
    28441 ccggtttccg tcaacaacaa taaattgagg tttgatctgc aaaccgtcga tggccttgtg
    28501 catggctaaa atagaggcat tcaatatgtt aattctgtct atttcttgat tatcgaccga
    28561 ggcaaccgac catgcaagtg cttccttttc tattattttt ctgaattttt ctctaagcaa
    28621 cgggctcaat tgcttcgaat cgttcaattg aggacaaatg aaatccggag gcaaaatgac
    28681 tgctgcagcc acaaccggac ctgccaaaca tcctctgccg gcttcgtcgc aaccacattc
    28741 gtgctgaaaa gaagaaaaac tccgaatgag gctgggttgt tttctatttg ttttgtcggg
    28801 gtacaatttt attttgctga tgggtatttt gttcatttgt cgattgcttg tccttcaatt
    28861 ctttccaaag ttccggccca gctttcaaaa caagggaata agtcaacgat tcttcttttg
    28921 tggctttatt tataaggtga aaccgttcgt ccattgcatc aaaaacattc agtaaaatac
    28981 ttagaacgag taaaaatttc aatccgccta ctatagcacc aagaaatttg ttcaatccac
    29041 ccaactgaat gattgacaag gttttttcaa acagctgagc caaaagaaac atagcaatgg
    29101 ccaaaagaat gaaactgaat aaataagcca atggacgagc catttgtgga gaaaaagaag
    29161 caacactttc aagcattttt gcaatcatcg gattcagcag cttggctccg tatattccca
    29221 caataatgac ggcaaaagaa gtcaattctt ttactaatcc tctaaacaag ccctgaatga
    29281 ttccgaaaaa aacagtaacc aaaataacca aatccagtgt gttcatcttt tgcctttaca
    29341 tatttttact gcaaatttat gaaaatagct ttcttgtttt catgttgaag aaaaaatttt
    29401 atagaaagat tcaaaaagca ataaataatt tcacttagtt tattgttatt cagttgattg
    29461 cattaatagg atagggccga ttgaaagttt ggcatgtgtt ttgattttaa atatcacacc
    29521 atgcatgagt gcctttttaa gatgtaaaga aatttgattt atcaaactta aaaaacttac
    29581 gattatgaaa acgaaaatat tgggtttggc tgctttatta ctgtttgcca cccagacgcc
    29641 ggcacaaagc acttttactg ttgaagctcg aaattacgat ataagcgata atctcgattt
    29701 acgcgcagtt gcttccattt ttgcagaatc aagagacttg tcggattttg aaatgagact
    29761 gaatgacgct cggtatcaga tttcaaatct tgacttgaac aacgatggat ggattgacta
    29821 cctgcgcgtg gtggaaacat cgggcgggaa tttgcatctt gtggttattc aggcagtgtt
    29881 ggacagatat acttatcagg atgtggcttc catagtggtt gaaaaacgaa attcacctca
    29941 agtatatgtg caaataatag gtgatcctta cctttttggt gtaaattatg ttattgagcc
    30001 gatatttgtt cgccaacctg taattttcag tttcttttgg agtaaaaatt atcggccgtg
    30061 gcattcaccg tattattggg gacattatcc aaaatattat cgaccactca gacctgtaga
    30121 agttaatatt tatctgggtc atgtacacca tatcataccg cgcgactaca aatttcatta
    30181 tcacaactct gtcaatatct attacagaga tgcttatgtg aggcttcacc aaacggtaag
    30241 aagaaatgat tttgagcgga cacatcccga aagaggtttc aacagcagga acaagaatgt
    30301 tgccaacaaa gccgagttaa acaattccag cagaagatcg tcaaccgaac aggaacgcag
    30361 aattgtcaat caggataatg gaagtagaag aatatctacc tcttcgagcg actcacgcta
    30421 taacaacagc gagaacaatt cttcaagaag aagtcaaaat accggaaggg ttgaaacaca
    30481 aaacaatgca agaagcattt caaataccgg tcgttcggta gaaacaagaa cgtcttcaca
    30541 aaacgaaaaa aattcatctc gaaccgaaac cctacgccga tcggaaaatt ctaccgtaaa
    30601 tacatcgcaa agaacttctg aaaacaattc aaggggcact cgttccacca actcaaactc
    30661 cgaaaatcgt tcaggcagaa gataaaaaca acttttgatt aaacttttca gaccggcaac
    30721 tcagcaacaa aaaatgcgaa tttgccggtc tgattttttg taaaagcttc aggatttttc
    30781 cataatttcc atttgatgtc ggatagattc ttcgtgaatg gcaataagaa cggttttcat
    30841 gaattcacca tccattccca tctcaatggc ttgctccaca cgattgtgca aaatttcatc
    30901 atatcgctgt gcctgaagaa taggcatgtt gtgttccttt ttatatctgc caatctcttc
    30961 agccacacgc atgcggtgag ccagcaattc gagcaaattg ttgtccaatt catcaatctg
    31021 acgacgtaaa gcagaaagat tttcggttgt ttgcatggtg tcgcgaataa ccagtgattc
    31081 taaaataaat tcaagcacct gcggagtaat ctgctgggca gcatcgctcc aagctttttc
    31141 aggattgcaa tgcgattcaa tcattaatcc atcgaagttt aaatccatag cctgttgcga
    31201 taatggagca atcagttcgc gtttcccgcc aatatgactc ggatcgcaaa aaataggcaa
    31261 atcaggcatt tgtcggcgca attcaatggg gatgtgccat tgaggcaaat ttctgtaaat
    31321 ttttttgtcg tacgaactga aaccgcgatg aacagcggct attttgtgaa ttccggcatt
    31381 ataaattctt tctatagcac ctatccacaa ctccaaatca ggattcaccg gatttttaat
    31441 aagtaccgga atatcgacgc cttccagcgc atctgcaatt tcctgaacag caaaaggatt
    31501 agctgtagtt cgagctccaa tccaaagaat atcaatatcg tgtgcaaggg cagcacgaac
    31561 atgtttggcg ttggcaactt ccacagtggt gtacaatccc aattccgatt tcagtctctt
    31621 caaccaggga agacctttct ctcctactcc ttcaaaaccg ccgggtttgg tacgaggttt
    31681 ccatataccg gcacgataaa tttttatacc gatagaagcc agcccgcgcg ctgtttccat
    31741 aacctgttct tcggtttcgg cactgcaagg ccccgctatg acaatgggac gtttggcttc
    31801 gattcccggc agtaaaattg attcaaattt cataattttg cttgttttaa taagtttaca
    31861 attccttttt ctaaaaaatc catatcaatg ttcgattctt cttaccactt cctccagtgt
    31921 aggttccgga caacataaag aaatgcgaac ataacgattt cccttgtctc caaaaataaa
    31981 tccgggcgta ataaaaacgt ttttcccata caaaatttcg tcggccaatt ctccggcgtc
    32041 tttgtaacga tcagggattt taccccaaac aaaaagtcct acctgccgag tatcataagt
    32101 acatccgagt aaatccagaa ttttaaaagc aatgtttctg cgtcgtgcat aaacaacatt
    32161 tatttgatcg tgccattcct ttggattttt caaagcttcg gcggcggcta tcatcatcgg
    32221 acggaacatt ccggaatcga catttgattt aactctcaat acccattcca caaattggcg
    32281 attggaagcc agcatggcaa tacgccaacc ggccatgttg tgcgacttgc tcatggaatt
    32341 catttcgatg caaatatctt tggctccgtc aacagacaaa atgctcaact ttcgatcatt
    32401 aagaatgaaa ctgtagggat tgtcgttaca aataataata ttgtgctttt tgccaaaagc
    32461 caccagtttt tcaaaaagtt ccacagaagc attggctccc gtaggcatgt tgggataatt
    32521 ggtccacatt aatttcacac cttccagatt cattttttcc aatgcatcaa aatcgggctg
    32581 ccattcatgg ttttcgtcta aatcgtaggg aataactttt gcttcgcaca aattgctgac
    32641 ggaagtatag gtgggataac cgggattggg aacgagtacc ccatcaccgg gattgagaaa
    32701 tgccaacgaa atgtgtagaa ttccttcctt cgagcctatc aacggctgaa tttcggtatt
    32761 gggattcagc tctacgccaa accatttttt gtaccattcg gcaaaaccgt tgcgcaattc
    32821 gggaatacct atataactct gataaccatg aacatttggc ttgtgcgctt ccttgcaaag
    32881 tgcttcgata gtagcttccg aaggaggaag atccggactt ccgattccca aattaatcac
    32941 atctttaccg gcggctttca tcgcgtcgat ttcttttagt ttggtcgaga aataatattc
    33001 cgaaacatta tttactcgcc tggcaggttc aatctccatt tttaattgtt ttaaaattat
    33061 aatagttgag ttataaataa gctgttaatt ttccgcatga tattccccca ttatttgaaa
    33121 ttcgtcgatt aagggaagaa tggcatcaat ggcttgttta tatcgcgaat aatcattaaa
    33181 tgtcaggttg atataaaatt gatattccca ttcttttcca atgatcggca aagattgaat
    33241 tttggtaaga ttcatgtgat agaaagcaaa aatagagaga acctgcgaaa gacttccctc
    33301 tacgtgcggc aatgtaaaaa ccagagaagc tttgttgatt tcatcgtttt tggtcatttc
    33361 caacgccagt gtcggttcag caacaataag gaaacgggta aaatttcgtt tgttggtttc
    33421 aatgtttctc tccaaaattt ccaatccata tttttcggcg gcaaaaaggc tgcaaattgc
    33481 agctcgctta taaatctgac catcagcgat ttctttagcc gacaaagccg tgtcttcact
    33541 ttctaccgct ttcaaatgct tgtgagattc caggaagtct tcacattgca tcaaagcaat
    33601 gggatgcgaa tgaacttctt caatttcttc gagcttctga tgaggcaatg ccaccaaatg
    33661 atgctcaatg tgcaactttt gttctcctac aacaagacag ccgctttcgc gtaaaagatt
    33721 gtgattttgc aacagactcc cggcaatggt gttttcaatg gcaataattc ccaaaagtga
    33781 tttatcggcc tctatggctt taaaaacatc cttaaaggtc tcacagggaa taatctcaat
    33841 ttcttctccc ttaaaatact cacgcgctgc aatttcgtga aatgctccac tgataccctg
    33901 aatggctact cttttcattt tatatgcctt aaaataaaaa atcctgctcg attgacttga
    33961 gcaggattct atattgtttt aaaaatttca ttttttcatg ccattttttt acatatcatt
    34021 atcctgctct taccttttaa agtaaaagta aaaataaaaa tatgtaaaga aacggctttt
    34081 cataaaaaat atcttgaagt gtttttgttt aacggtgcaa aagtaaaaat aattttgagt
    34141 taaaacaaac aaattgataa aaaagtttca aaaaataatg aatttgttat ttagacggaa
    34201 attcatcaat aaatttcagc gctttactca tggcagccga aagccctgca gcattctttc
    34261 caccggctgt ggcaaagaaa ggctgtcctc ccccgccccc ttgaatttcc tttgcaattt
    34321 ctcgaattat ttgagcagca ttcaatcctc tgcttaccaa atcgtcagaa agtagcaatg
    34381 ttaacgaagg tttgccatcc atcacattgc ctgcgatgaa aaagagattt tcgttctgca
    34441 gatttttaat ctggaatgcc aacgttttag cctgatcagc agtcaaattt ccttcgtagc
    34501 gaacaacatg aatcccattt gttatcttcg cttcagccat aagccggtcg cgaagttgaa
    34561 gcaatttttc ctgttgaaag gtgttgacct gttttttcag ttccgtattt tcttccatca
    34621 gctttctgac ggcgtttacc aaattaccgg gattgttcaa tatttctctc gtttccgaaa
    34681 gaatgtcctg ctgctcatcc atcaattttt caacagcttt tcctgttatg gcttctattc
    34741 tgcgaattcc tgccgcaatg gaactttcgg aaattattct taccattccg atttctccgg
    34801 tggcttttac gtgcgttccg ccgcaaagtt ctttagaact gccaaactga atcactctca
    34861 ccgtttcgcc atatttttcg ccaaacaatg ccattgcacc catttctttt gcttcgctaa
    34921 cagggacttc ccgcatttcg ttaagtggaa gattttcacg gattttcctg ttggcaagcg
    34981 cttcaacggc acgaatttct tctttggtca ttttttgaaa gtgggaaaaa tcaaaacgca
    35041 agctgtccgg tccaacatac gaaccttttt gctccacatg cgttcccaaa actgttctca
    35101 gagcttcgtg cagcaagtga gttgccgaat gattacagga agtggcttgt cggttttcaa
    35161 gatgtacaac ggctgtgaaa gttcccgtaa tttctgacgg aagctgtttg gcaatatgaa
    35221 ccgcaagatt attttccttt ttggtatcaa gaatttctat tttttcgttt tcacccacta
    35281 accagccgct gtcgcctacc tgaccaccca tttcggcata gaatggcgta acagacaaaa
    35341 caagctgata aaattcctga tttttctgtt ttattttacg ataacgaagt atgttcgttt
    35401 cgcacgaagt gcaatcgtag ccaacaaagt ggctgtcgcc ttcacgcaaa acaatccaat
    35461 cgcccgtttc gatattggct gcatttcggg ctcgctcttt ctgcttttgc atttctgcat
    35521 gaaactcgtt catgttgacg gtaaaattat tttccctcaa aatgagctcg gttaaatcaa
    35581 tgggaaatcc aaaagtatcg tacagaacaa atgcatcggc accggaaatt tcttttttgt
    35641 tttccaattg agcctcttcc atttttcttt ccagcaatcg gataccggtt tcaagcgtac
    35701 gtaaaaaaga ttcttcttct tcctttatta ctttttcgat cagtgtcttt tgactgatca
    35761 gctctggata ggctttcccc attgtttcgc acagaacgtc aatcaaccga tacataaaag
    35821 gagcgtgttg atgcagaaaa gtatatccgt accgcacggc tctgcggaga atgcgtcgaa
    35881 tgacatatcc ggctttcatg ttggaaggca gctgcccatc ggcaatggaa aaggcaattg
    35941 cccttatatg atcggatacg acacgcatgg cgatatcgat tttctcgtct ttaccgtatt
    36001 ctgtaccact gattttccct atttcgcgaa taatgggttg aaaaatatcg gtatcgtaat
    36061 tggaagtttt tccctgaata accatacaca gacgttcaaa tcccattccc gtatcgatta
    36121 ctttggcggg aagttcttcc aaagtgccat cggcttttcg gttgtattgc atgaaaacat
    36181 tgttccaaag ctcaatcacg agtggatgtc cctgattgat aagcgaaagc ccgtcaagtt
    36241 ttgcgcgttc ttcctcgctc cgaatgtcaa tgtgaatttc ggaacacggt ccacagggcc
    36301 ctgtttctcc catttcccaa aaattgtctt ttctgctgcc attgatgatt ctatcggcag
    36361 gcagaaattg cgaccaaatg gcggcagctt cttcatctct ttcgagccct tcagatggag
    36421 aaccttcaaa aacagttaca tataatctgc ttttatcgat tttcaacact tcggtgagat
    36481 attcccatgc ccattcgatg gcttctttct tgaaatagtc gccaaacgac cagttgccga
    36541 gcatttcgaa catggtatga tgataggtat cgtgcccaac ctcttccaag tcgttgtgtt
    36601 ttccactcac ccgaagacat ttttgcgaat tggctacgcg tggatatttt acgggatcat
    36661 tgcccaaaat tatgttttta aactgattca tacctgcatt ggtaaacatc aaggtaggat
    36721 catctttgat aaccattggt gccgaaggta caattttgtg gcctttctct gcgaaaaaat
    36781 caagaaaaga ttgtcgaatt tcctgtgaag tcattttgcg aaattaatta aatccatttt
    36841 cctttgagtg aatgcaaatt ttaccgaagg ttaataatct caaataaagt ctgcaaaaat
    36901 acgccaaatt tattattttt gcactatcat gcttgataaa aagttgaatt tggctcattt
    36961 atctattctt ttttcaaaat tttagcgctt tttttatgtt cctaaatcgt tttctatggc
    37021 aaagaaaaaa tatcatttca atcccgatac tttaagttac gaaccgatag aaaattcact
    37081 gtggttcaaa atacgtcgtt ttttgtttca cgcttttacc ggaattgcaa caggaacggt
    37141 ttttttcttc attttggctt caacagttca atctcctgaa gaaagagaat tgaatgcaga
    37201 aaaaaacaaa atggaggctc aatacaaagt gcttcaacgc cagttcaatg aagtacagga
    37261 tgttttgacc gacttggaag agcgggacaa caacttgtat cgtgttgtac ttcaagctga
    37321 accgattccg tttaccgtaa gaaaagccat tgcatcgaat accgattatt acaaccagct
    37381 ttacgacatg acgaattctg aaattgtagt cagcacaacc aaaatgctga ataccctgaa
    37441 aaagcagctt tatattcaat cgcgttctta cgacgaattg gtaaacctta caaaaaacag
    37501 ggaaaagatg ttgcaatgtc ttcctgccat tcagcctgtg ctgaacaaag atttaaaaag
    37561 aatggcatca ggttatggtt ggcgcatcga tcctgtttac catacgcgtc gctttcatga
    37621 agggatggat ttcgttgctc ctacaggtac cgatgtgtat gcaaccggcg atggcactat
    37681 tgtaaatgcg ggctgggaac agggttacgg aaactgtata gtaattaatc acggatttgg
    37741 gtacgaaaca ctatatgccc atttaagcac catcaaagtg agaccgggtc aaagtgttaa
    37801 acgaggagac attatcggac tggtaggcag tacgggcaaa tcaactgctc ctcacctcca
    37861 ctatgaagta cattataagg gacaggttat gaatcctcaa aattattatt tcctggattt
    37921 gtcgcctgaa gaatacgaca gaatggtaca aatgagcaac aatttcggnn nnnnnnnnnn
    37981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38041 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gatttgtcgc ctgaagaata cgacagaatg
    38101 gtacaaatga gcaacaattt cggagaagtt ctggattaat aaaccatttt ttgaaaaatt
    38161 tcacagagaa tgaatgaatt acaaggaaac aaaaattatt cgcttacgaa gttggaatta
    38221 tcatcgttgc ccggtccgat cggcgtgttc gattcgggat atggcggctt gaccatattg
    38281 gaaaaaatcc gtcaaaggct tccccattat gattaccttt acttgggcga caatgcgcgc
    38341 actccttacg gaactcgttc ttttgaaatc gtttataaat atacgctgga atgtgtcgtt
    38401 cggctgttcg aattgggtgc ccatttggtg attttggctt gtaacacagc ttctgccaaa
    38461 gcattaagaa ccattcagca aaatgatttg ccgaaaattg atccccgcag gcgcgtactg
    38521 ggaattattc gacccacagt agaaataatt gataaactga cacacaatgg tcatgttgga
    38581 ttattggcca ctccgggcac tgttcagtca aattcctacg gactcgaaat acaaaagttg
    38641 tacggaaatg ctattaccct ttcttccgaa gcttgtccca tgtgggtacc gctcatagaa
    38701 aattttgaac acaattctcc cggagccgat tatttttttc aaaaaaatat caatcacttg
    38761 ttacaaaccg atccacagat tgatacggta attttgggct gcactcatta tactttgctt
    38821 caggataaaa ttaaaaatct tcttccgccc aacatcaata tcgtttcgca gggagaaatt
    38881 gtggcgtcaa gccttgatga ttacctgaac cgacatcccg aaatcgacgc actttgcact
    38941 aaaaatgctt cctgtcggtt tctgacaacc gaatcggtgg aaaaattttc ggcttctgct
    39001 acaatttttc taaacgaacc cgtactggct gaacatattg acatcgatta acagcatttc
    39061 tgtttcaact ctattaaaga tttttcatgg aagatgaacg atttgctggg aaaacaataa
    39121 tttcattaac cggaaattaa tttttatgcc gatttttctt tttgaaattc aatgatttaa
    39181 cttgtgtttt aaaacaatgt tttataacat aaaaatttta tctccaaatt aatggacttt
    39241 ttataccttg tcgattcgat ttttacaaag ttaaaacata attttatagt attaatcaat
    39301 taaaaattaa taagttatga aagtatatca gcctaatgaa ataaagaata tttctttatt
    39361 gggaagttct gggtcgggta aaaccaccct tgtggaagct atgcttttcg agggtggaat
    39421 cattaaacgc agaggcacca ttgccaatca gaacacagtg tgcgactatt ttccggtaga
    39481 aaaggaatat ggatattcgg tattcccaac actggcacag attgaatggc atcaacataa
    39541 acttaacatt attgattgtc cgggttctga cgattttata ggtgctgtgg taacatcact
    39601 taatgttacc gatatgggat taattgttgt aggagctcaa tatggagtgg aagttggcac
    39661 caacaaccat ttttcttatg ccgaaaaata caacaaacct gtcatgcttg ttgtgaatca
    39721 gttggatcac gacaaagctg attatgaaca tacccttgaa cagctgaagg aaaattttgg
    39781 aaataaagtt attcagctgc aatatccatt acagacagga agtggattta attccttggt
    39841 tgatgtactg caaatgaaaa tgcttcgttt caaaactgaa ggtggagcac ctgaaattac
    39901 cgaaattccc gactccgaaa aagcaaaagc tcaagagctt cacaatagtc tgattgaatc
    39961 tgcagcagaa tttgatgaaa gtctaatgga aaaatttttt gaagaaggaa tactttcgga
    40021 tgaagaaatt tataaaggtt tgtataaagg tgtgttggaa aggggcattt ttccggtaat
    40081 ttgcgtttct gccgccaatg atgccggtgt aagtactttg atggattatt tggtgcggat
    40141 tgctccctcc gtttccgaca gcaaacgccc cttgactaaa gatggaaaaa gtgtagatcc
    40201 aaatccagat gggccaacga gtatctatgt ttttaaaaca aacgttgagc cgcatattgg
    40261 agaaatttca ctctttaaag ttatgagtgg aaaattgaag gaaggagatg acctttacaa
    40321 cgtaaaccga gaagtaaaag aaagaatcag ccagattttc tgcgttgccg ggcaaattcg
    40381 caacaaggtg gaagaagttt cagccggaga cttcggagca acggtaaaac tgaaggatac
    40441 ccgcacagga aacactttga acggcaaggg ttgcgattat gattttgtcg gagtagaata
    40501 tcccgaaccc aaataccgcc gggctattaa agccaaatcg gaagctgatg aagaaaaatt
    40561 gaacgaaata ctcactcgaa tgcacgaaga agatcccaca tggatagtag agcagtcgaa
    40621 agaactgaaa caaaccattt tgcatggtca gggagaattt cacttgcgca ccttgaaatg
    40681 gagagtcgaa aacaacgaca aaatagaaat tgaatttaaa gaaccaaaaa ttccgtatcg
    40741 cgaaaccata accaaaaaag cccgagccga ttaccgtcat aaaaagcaat ccggcggtgc
    40801 cggacagttt ggggaagttc atctaattgt tgaaccgtat ttcgaagatg ttcccattca
    40861 ggatgtgtat cgtttcaatg gtcaggaata caaaataagc gttcgtgatg ttcaggaaat
    40921 agatttggaa tggggcggaa agctggtatt tgtcaacagt attgtaggcg gtgccattga
    40981 tgcacgattt ttaccggcca tcctgaaagg tgtaatggat cgtatggaac aaggaccact
    41041 aacgggttct tatgcgcgtg atgttcgcgt cattgtttat gacggaaaaa tgcatccggt
    41101 tgattccaac gaaatttctt tccgattagc cggaagaaat gcttttgcac aagctttcaa
    41161 ggacgccggc cctaaattgc tggaaccggt ttatgatgtg gtggtaatga ctccatcgga
    41221 cagattggga gatgtgatga gcgatttgca aagccgtcgt gccgttatca tgggtatgaa
    41281 cagcgaaaaa ggttttgaaa agttgcaagc aaaaattcct ttgaaagaac tgagtaatta
    41341 ttccacaacc ttgagttctt taaccggtgg aagagcttcg tttgtaatga aatttgcaag
    41401 ttacgaactc gttcctatcg aaattcagga aaaactgttg aaagaatatg aagcttctaa
    41461 gaaagaagaa gattaacttc taaattgaat tttaaaaaaa ggggcaattt actttgaaag
    41521 tagattgtcc ctttttagta ttataattca attgttggat taaccgtaaa taattttgcc
    41581 atcgtgagtt tgacttcctt caaagctttt gttgtactgc tgcaatgcac gataactcat
    41641 acccatgctc gaaaagcctc catcgtggaa aaggttttgc atggttacct tgcgtgtcag
    41701 gtcgctaaac attactacac aataattggc gcaatcatct gctgaagcgt ttcccagtgg
    41761 cgacatgcgt tccgaaaaat ccattaaact gtcaaatccc ttcactccgc ttccggcagt
    41821 agtaggagtt ggcgactgag aaatagtgtt gatacgcaca ttgtgttctc ttccgtaaat
    41881 gtatccgaag tttcgggcta tcgattccag catggctttg gcatcggcca tatcgttgta
    41941 tccatacatc gttcgatgag aagcaatgta ggaaagagca acaaccgaac cccactcatt
    42001 gatggcattc atttttttgg cagtctgaag cattttgtga aaagatatgg ctgaaatatc
    42061 caatgtggtg tgcatcaaat cataatccaa atcatcatag ggcctctttt tcctcacatt
    42121 tggtgacatg ccaatggaat ggagaataaa gtcgatttta ccacccaaaa tttccatcga
    42181 ttgagagaaa acttgctcca aatcatcaag attggtagca tcggcgggga taattttggc
    42241 attgattttt tctgccaatt catttatggt tcccatacgt atggcaattg gcgtgttgga
    42301 cagggtaacg gtagctcctt cttcgacaac gcgctcggca actttccagg caatcgacat
    42361 atcgttcaga gctccaaaga tgattcctct ttttccaact aataaattat tactcatagt
    42421 ctttaatttt aatcgtaccg ggaaaaattg aaaataaacc ggtatgaagg ttgttaatga
    42481 taaataagtt ggctgcaaaa gtaatcaaat tgttacattt tagcaatttt gattttgaaa
    42541 gtgatctttt tataattgta aatacctata aatgaattga ttgaataaaa ttagcgccgt
    42601 aagaacttcc aactctaaaa atagataatc taaattattt gctcaaaata aaatcacatc
    42661 ttctattcat tctataggct ctgggaggtt gagggatttt tccctttcca ttggaaataa
    42721 ttctctgcgg attgatgtta tgggtgaaaa ccaattccgc ttttacgcga ttggagcgtt
    42781 cctgacttaa tcttttgttg tatttgttac ttcccacatt atcggcatat cctctgattt
    42841 ctaccaataa ggttggatca gtatttaatt tttctgccag tttttcaatt tctattaatg
    42901 cttcttcatc caaattggtt ttgtcaaagt caaaatacac agaggcaaaa gcgcatggtt
    42961 tatcttgagc tatgcttcta ccccaaaaat cgacacacag attttttggt gaattaggct
    43021 ctttgtctcg gttgtcgggg acaccatctt tatcttcatc ttttgttgaa gcacttatat
    43081 attgaagata ttcaggatga atctcttggg tttcggggat tacttttatt tcaggaatgc
    43141 cacgtccgtt ataatcaatg taaagcccct ttggtgtatt gggctccaaa tccctgctat
    43201 tggggacgcc atcgccgtca tcatccggtc ccggttgcaa acttaatttc aacttatcga
    43261 gttcttgagc cagcgaatct gttttgatgt ttgcgcgatt tgctaaagtg aaaacatctc
    43321 ctaaagtaat tcttgtttcg ttgttgcgta tgtgattttt actttttgct ccaattttcc
    43381 aacgcaacga aagagctgcc attgcaatat tgtcgttggt aacaccttta aaattatatc
    43441 ctccggcttc ttctttcaaa ttggttcctt caaagttatc tttattctgc aaacgatact
    43501 gcacttttgc acctatagca aatagtgacg agatattata ctctatcaaa cctcctaccg
    43561 ggaaaactgc agcccatcca tctcttattc tgtcaaactc aatgccattt ttatacaaag
    43621 tagaattgta ataggccact ccagcgccta ccgtagcata aatattccat ttggaatgat
    43681 tattttcatt acataaatta agcagattca acgaaaggta aggagtgaaa tgaaatacat
    43741 ctgcgttgaa ttttttggca gcatcttctg cagaaacggg aaaataaaaa aattcagttc
    43801 caatgcccca aaaaggacta agagtaagct ctgttgaaaa tccaaagctt gcctttgctg
    43861 cagaattcgg tataatctgg ttataatttt gattattgtc tccgtcaaaa ttattgattc
    43921 ctgcatccaa ggttattgac catcgcctgt aatcatttgg ttgtccataa gaggagatga
    43981 aaatggacaa aatgaaaaag ccaacaataa attttttcat ggttataaga tttattccaa
    44041 ctgaatttag aatttaatat taaaacttgt attcgcaaca aataaaataa cttatgcaaa
    44101 accacaaaaa taatattaaa attttcaaaa acaaaataat tttaatcact attaaccgac
    44161 aaaaatcaga atttgtcaat tttaatttct aaataattga ataatagttt attaatatgt
    44221 tttctctgtt gactatagtc agtttatgcg aaagcataga tttctccatc acaaatgaaa
    44281 aatctttaca atccacccga ctcattcccc aatccatgcg ttttcaggaa tggagagaat
    44341 aaggatagtt cttgtcaaat ttttcttcag cttatttgat ttttctgcct aaatgaccat
    44401 atttcagcca gttgaaattg ccaaaatgac taatttccac gtttggtatt tattttgttt
    44461 ttaacttagg agcaaaaaaa tatgtttaac caatcaaagt gatggagggc tgatatatgg
    44521 caactaacag actaaccgaa caattaccat caaattttga ccgttatttc gataacgaat
    44581 gggaagatta tttctggact gacagggaat tggcggcaaa tcatactact ttaccggcag
    44641 ttaacatcaa agaaaatggt gaagctttcg aaattgatgt ggcggcaccc ggttacgaaa
    44701 aaagcgatat taaaatcgaa atgaacgaag atttgctgac cgtttcttcc aacaaaaaac
    44761 ttgatgaaga aaaaacggaa ggtgaatata caaagcgcga gttcagttat cagtcgtttt
    44821 tacgaacatt tactctaccc gagacagttg atgcagaaaa aatttcggca gtatatgaaa
    44881 acggaatttt gaaaatcacc attccgaaaa aagaagaagc caaagtaaag ccggcgcgga
    44941 cgattgatat tcaataacaa aaggaaaaaa ttagattttt tagatcagct tttttactat
    45001 cgaaacaaga aagaaaaata tatggaaaca tttcaggata ttatcaacgg agataaaccg
    45061 gtattggtag atttttttgc gacgtggtgc ggaccatgta aagcgatggc tccggtgatt
    45121 gaaagtctgg gcaaagaagt tcaggggcaa gcccgtgtgc taaaagtgga tgtggacaaa
    45181 aatcaggcat tggctgccaa atatgggatt caggcagttc ctacctttat gctgttcaaa
    45241 aaaggaaatg tcatttggaa acattccgga gcaatagata agaacacctt gatgcagcaa
    45301 ataaaatctg ccgtataaca agatcaaaac aattgtggaa ggaaggtaaa aacattttgg
    45361 agtacaaaat actcttctat ttctcaaaat gtgtttacct ttttctttta aaaaaattct
    45421 gcatcaatgt agcacattct tctgccaaca ctccagacac tacttctgtt ttagggtgaa
    45481 gagcatctgg agcaaatttt gaatatccgc gtttttcgtc ggaagctccg taaacaattt
    45541 tcttgatttg tgcccatccc agggctccgg cacacataac gcaaggctct atggtaacat
    45601 acagggtaca gtcggacaga tatttacctc ccaaataatt ggcagctgaa gtaatagcct
    45661 gcatttcggc atgagccgta acgtcattca aagtctcggt gagattgtgg gcacgagcaa
    45721 ttatacttcc ctgacataca atgacggcac ctatgggaac ttcatctttt tcggctgctc
    45781 gccgtgcttc cgcaagcgct tgtttcatat atttaatatc ctgattttct tccatttgtt
    45841 tatcctttca ataaaatatg aattttgaaa gcaaaggtaa aaaattttct tcccgtttca
    45901 attcagatag accgtttgat aatttttgct cttgataatt ctatgagttt tagatttaac
    45961 aaaaaaaccg aaattttatt cgtcggaatt tttgtaattt tgtaatttga aaaacttaca
    46021 tatgaaaatc ataattgtcg gcacagccta tccttaccga ggcgggttgg cttcgttcaa
    46081 cgagcgtttg gcgcgtgaat acgccagaga aggtcatgaa gtggaattga tcactttcac
    46141 tcttcaatat ccaaattttc tgtttccggg aaaatcgcaa tattcaacgg aaaaaccacc
    46201 cgaagacctt aaaattatcc gccgtatcaa ttcgataaat ccatttaact ggataaagat
    46261 tgggaaagaa atcaagaaga aagctcccga tttggtcata ttttgctact ggacttcttt
    46321 tatagcaccc tgttttgcaa ctatcgcacg atatgcgcgt tctccacaaa ctaaaatgct
    46381 cggtttggtt cacaatatga ttcctcatga agcaacacca ctggacaagt ggctgccggg
    46441 atatttcgta aaaaatatgg atggttttct ggcaatggca aattcagtga aagaagatat
    46501 tcaacatttt gacaaaaaaa ataaaccaaa aattgttgtt cctcatcctg tttacgacca
    46561 ttacggtgca attgtatcga aaagcgaagc agcattgaaa ctcggcttgc cccccaaaaa
    46621 aagatatgtg ttgtttttcg gattcattcg ccattacaaa ggcttggatt tactcctgga
    46681 agctttttcc gataaaagat tgaaagatat ggaagtgcaa ctggttgttg ccggagaatt
    46741 ttacgaaaat cccgagcctt atttaaaaca gattgcacaa ttgggactgg ccaacagagt
    46801 tgaactaaga accaatttta ttcctgaaaa tgaagttaaa tactatttca gccttgccga
    46861 tttggttgca cagccctatc gttcagccac tcagagtggg gtgagccaaa ttgcctatca
    46921 ctttgaaaaa ccaatgttgg ttaccaatgt gggaggcttg gccgaaatta tcccaaatgg
    46981 aaaagttggc tatgtagtaa acggacaacc caaagaaatt gccgacgcca tagttgattt
    47041 ttttcagaac aaccgggaag aaatattttt gcccaacata aaaacagaaa agatgaaata
    47101 cggttgggat aaaatgactg ctgccatcaa caaacttttt ttcaagctct gaaaaatttt
    47161 tcccctgccc cgacttttca taatttcaat tagtagaaca tagagaaaaa nnnnnnnnnn
    47221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaattgccga tatatgtctg aaccaattgt
    47341 agaaacgcct ctaatgaaac agtatttcga gatgaaatca aaacatcccg atgccatttt
    47401 attgtttcgt tgtggagatt tttacgaaac tttctcaact gatgctatcg aagcttcgga
    47461 aatattggga attacactca cgcgccgtgc caacggatcg gcaaaaagcg tagaattggc
    47521 aggatttccc catcatgcgt tggatactta tctgccaaaa ttggtgagag ctggaaaaag
    47581 ggtagccatt tgcgaccaac tggaagaccc caaactggca aaaaaaattg taaaaagagg
    47641 catcaccgaa ctggttacgc ccggcgttac ttacaacgac accatcctca accacaagga
    47701 aaacaatttc ttagcgagcg tttacctcaa caaaaatcag gcaggtgtgg cctttcttga
    47761 tgtttctacc ggagaatttc tgttggctga aggaacaaac gaatacgtag ataaattgct
    47821 gaacagcttc tctcccaaag aaattatttt cgaccgtacc aaaaagaaag aatttgaaaa
    47881 tttatttgga accaaatttt attcctatcc gctcgaagat tgggtattta cccttgatgc
    47941 agccaaagac aggttgctga agcagtttga aacgaaaagt ttgaagggtt ttggtgtgga
    48001 aggacttgcc aacggagtgg ttgcagccgg cgccattctt tattacctcg acctgaccca
    48061 tcatcaacaa acgggacata ttacccaact ctctcgcatt gaagaagacc gctttgtatg
    48121 gcttgacaga ttcaccgtaa gaaaccttga actttttgaa gctatcaacg aaggaggaaa
    48181 aaccttgctc gaagtgctgg ataaaaccat cagcccgatg ggcagtcgac tgctgaaacg
    48241 ttggatggct tttccgttga aaaatgtaaa atctatcaac gaaaggctgg atgtagtaga
    48301 atattttttc aaacatccgg aattgaaaga agctttggct cagaatattg cgctgattgg
    48361 cgatttggaa cgtctggttt ctaaaattgc tgtgggcaga atcaatcctc gcgaagtggt
    48421 tcaattgaaa acggcgctca aagccatcga accaataaaa caattgtgta ctcatacaga
    48481 aaacatcact ttgcgcacca ttgccgacca gctgaatgcc tgctccatta tttccgaaaa
    48541 aattgaaaga gaaattcaaa acgaccctcc catactcatt aatcggggaa atattattca
    48601 aaaaggtgta aatgccgacc tcgacgaatt gagagaactg gctttttcgg gaaaagattt
    48661 cctgcaaaag attcaggaac gtgaaagcca agccacagga attccaagtt taaaagttgg
    48721 attcaacaat gtttttggct attatctcga agtacgaaat acctacaagg aaaaagttcc
    48781 cgaaacatgg attcgcaaac agacactggt aaatgccgaa cgctacatca ccgaagaatt
    48841 aaaaaaatat gaagaaaaaa tactcggggc agaagaaaaa attattgata ttgaaacccg
    48901 tttgttcaac gaactggtgt taagtctttc cgaatacatt cctgcccttc aactcgattc
    48961 gaacttgata gcccagctgg atggtctgct ttcctttgcg aaagtttcgg aagaaaaccg
    49021 ttatgttcgc cccgaaatca acgatactga agtgctggaa attcatcagg gcagacatcc
    49081 ggttatcgaa aaacaacttc ctttgggcga aagctatgtg gccaatgatg tgtatctcga
    49141 cagctcgaaa cagcaaatca tgattattac cggccccaac atgtcaggca aatcggcttt
    49201 acttcgacaa acggctttga ttactcttat ggcgcaaatg ggatgcttcg tgccggtaga
    49261 aagcgccaag attggcgttg tggacaagat ttttactcgt gttggagcca gcgacaacat
    49321 ttctttgggc gaatcgactt ttatggtcga aatgagcgaa gccgcgagta ttctcaacaa
    49381 tctttcctct cgcagtttgg tgctttttga tgagctggga agaggaacaa gcacttacga
    49441 tggcatttcc attgcttggg caatagttga atatattcac gaacatcctg ctcacaaagc
    49501 aaaaacgctt tttgctaccc actatcacga gctgaatgaa atggaaaaat ccttttcgcg
    49561 ggtgaaaaac tacaatgttt ccgtaaaaga agtggacaac aaggtgattt tcctccgaaa
    49621 actggtgcga ggtggaagtg aacacagttt cggtattcat gtggctaaaa tggccggtat
    49681 gccgcaaagc atcgttaaac gcgctgaaca aattcttaaa cagctcgaaa cggataaccg
    49741 acaatccgat atttccaaac cggtagataa aattgcccag cacagggaag gttatcaact
    49801 cacttttttc caactcgatg accctgtact cgaacaaatt agagatgaaa tcaagaattt
    49861 ggacataaac aacttgactc ctatcgaagc ccttaataaa ctgaacgaaa tcaaaagaat
    49921 tattatggga aaataaaatt tatctgttca taaccttcga tgaattttaa agataatcat
    49981 aattggtttt ttaaaaaatt tcatcttgat tattgactta taattctaaa tttatgagtt
    50041 tttaaaacaa gtgtttttat ttgctatttt aaactaacct gttccaatat atctacaacg
    50101 tcttcacgag taattcttct aaaatgttct tcaacgggtg taataaaaat accgctcatt
    50161 tctacggtag ccggacttac cattaattgc cggctttctt cagccgaata ttgccacgga
    50221 cggaatgctt ttcgcggaaa aacaaaaacg atccacttct gattttcaaa caggcaaact
    50281 acattcatca tgggttcttc gtttgcctga caattcattt ttttctgaaa atcttcataa
    50341 agccgattga attggaagga caaaacttgg ctatcatccg actccataca ataaaccgtg
    50401 cgtaaataat ttttaaaaaa agataaactg taatggtttt ctttctccaa tatttcggca
    50461 tgcgtggctt ttaatcggcg ataatcattg accagaggca aaaaattttt tgtcccggcc
    50521 tgaaaatgca tgtgatcggg agcagaagcg ccacattttg ggccattgta aaaaacaaca
    50581 aactcgtcca gcgactttgc caattcgagc atatcgggga aaaaaggttt tatttgctgc
    50641 ggaatatgtt ctttgcgggg aatggtaaaa tgctcgggaa aaatagggta ggggtttacc
    50701 aaaacagtat aatcctctgc atcgacacat ttttgttgtg gtggcagatt ctgacgacac
    50761 aaaaaacatt tcctttcctt tatggtcttt tcatctactt ttgcacccga cgaaacaatg
    50821 cgtgccgggt taaactgcac tacaatttca aaatcgccaa aagaaaaaac cttaatttca
    50881 acttttttca aatcttcgta attctttttg gccaactccc attcagaaac ttgctctttg
    50941 aacaagtttt tcacctttaa attcaaattt cccataaaaa attattctgt ctaaaaattt
    51001 atttcctcaa aaattgaaat tggactttaa gctttttttc tgtaactatt gatagcccaa
    51061 aaattgaaaa aaatagcaaa accgaccaaa gccataaatt gatgccacaa atccataaaa
    51121 ctgctgcctt tgagataaat ggaacgcaga gcttcaatca tgtaacggag cggactgaaa
    51181 aagctcatcg tctgagtcca ctccggcata ctggaaatag gcgtgtaaag cccactcaga
    51241 aatataaatg tgatgacaaa aaagaacatc ataaacattc cctgctgaat agtggtagca
    51301 tagttggaga taaccaaccc cagacctgag aaagccaata caaaaaccgc tgcgaaaaga
    51361 taaatttgca gataacctcc ttttgaaatc attccgtaaa acagccatgc cacaaaaaaa
    51421 catattgtaa gaacgataaa accaatgacc caatacggaa taagttttga aagaatgagc
    51481 gtgaacttat ttaccggagt tacattcatc tgctcaatag ttcctttttc tttttcccct
    51541 acaatattca atgccggtaa aaatccggtt atcatggcca gcatcatcat cataatggcc
    51601 ggaatcatgt tgtacttata ctccagatga ggattaaatc ggtattgcgg aataatttcg
    51661 caggctggaa cttttagttt tgtaccactc tgaatccatt cttctctgat ttcactgctg
    51721 aaatcggtta ttattccgac taaataggca ctgcttagtc cacctttcat tccgtttacg
    51781 gtgtttgctg aaatcagcac actggcagta aggttattta ctaagtcttt ttcgaattga
    51841 gccggtattt cgagtataac atcagatttg ttctgttcta cacttttcag tgcatctgag
    51901 taattatgcg aaacatttgt caggataaaa tgccccgacg aaatgacctt ctgaatcaga
    51961 cgttcggagt actgactatg gttgttgtca accacagcaa gattaatgtt tttaacatcg
    52021 aagttggcta ccaatgggaa tagcgcgagt gcaacaaacg gcaagatgat gaccatacgg
    52081 ggcaaaaact tgttgcgcat aatttgcttg aattcttttt ctaataaata tttcagcata
    52141 attttgtctc ctgttattat tccaacctat acttgaaatt tttcagactt gctatcacca
    52201 acacgattcc cattcctaat aaaacactca tttccggaat gatggaagat aaaccaagtc
    52261 ctttaatcat gatttttttg acccctataa tataccattt cgcggggata atttgcgaaa
    52321 tccattgaaa gaaaaccggc atattttcga ccggaaacat cattcctgat agcataatga
    52381 ccggtaacat aagcaccatt cccgagataa gcagagccac cagttgcgag tctactatcg
    52441 aagaaatcaa caaccccaac gatagattta caaaaatata gaccagcgag aatgcaatca
    52501 accagaacaa gctccctgca attggaacct tgagcaggaa gacggataag agtaaaatgg
    52561 tagctaaatt taccagcgac aaaacgaaat agggaatggt tttggaaagt atgatataga
    52621 taggtttcat tggtgataca agcaaaactt ccattgtgcc aagttctttt tcgcgggcaa
    52681 tggagatgga agtcatcatg gcacaaatca gcataagtat catacccatt acacccggaa
    52741 caaaattgta agcacctttc atttgtgggt tgtagagcaa ttttatttgt ggatttattt
    52801 gaaacggaat cttatcaatt ttagatagtt ccagttggta agctccaata atactggttg
    52861 cataagtcac aattgttttg gccgaattag gatcactgcc atcaacaatt agttgtactt
    52921 gtgcgttgcc cgtatgtgcc agattttcat aaaagttatc accgaaaacg agtaccatat
    52981 tcagtctgtc ttttttgaat aaatcctgaa tttcggacgg attatttacc attttcttga
    53041 ctgaaaaata ctcgctgttc ctgaattttt caataatagc gcgtgttgcc acatcatttg
    53101 aagaatctag tatggcaatt ttagaattct tgacttcggt gctgattgaa tatccaaaca
    53161 acacaagcat cagaatgggt aaaatcaaca aaatagccat tgttgtttta tccctgaaaa
    53221 tatggtaaaa ttcctttcga acgaaggaca tgaattgttt catactaatc tgcctttctt
    53281 gaagcgcgtc gcgcaagttg ttgaaaaact ccatccatat tgttggcttt aaactggttt
    53341 tttagatttt ttggagaatc gagagcgtca attcgtccat caaccataat ggaaacccga
    53401 tcgcaatatt ctgcttcgtc catataatgc gtggtaacaa aaacggtgat tccgcggtcg
    53461 gcggcttcgt aaatcatttc ccaaaactga cgacgcgtta cgggatccac tccgcctgtt
    53521 ggctcatcaa gaaaaactat cttaggctca tgaaaaatgg caaccgaaaa ggcaagtttc
    53581 tgcttccatc ccaaaggaag cgatttcaca aacgaatttc tttctttttc aaatccaagt
    53641 cgttttaata actcatctgt tttttttata atttcatctt caggcacgcc ataaattcca
    53701 ccatacaaac ggatattttc ccaaaccttc aaatcctcgt acaatgcaaa tttttggctc
    53761 atgtagccga tgtttctttt cacttcttcc gattgggtat gaatatcgaa accggcaaca
    53821 agtccttttc ccgaagtggg tggcaaaagt ccgcacagca tacgcatggc agtagttttt
    53881 ccagctccgt tggcgccgag gaaaccaaaa atttctccct tgtacacctc aaaggaaata
    53941 tgatcaacag ccgtaaaatc accaaatttt ttggtcaaat tttgtacttc aataactttt
    54001 tccattctca attattttct tcctgttgtt tggaaagctc cataaaacaa tcttcaacag
    54061 ttggttctat cttggcaatt tcgatttgtt catgtccgtt ttttaaaaga aaattctgca
    54121 attcctcttc tgaaattttt ttgttttcta ccgtaacgtg aatactgtca ccgaaagcat
    54181 aacagctcga aacctccggc aaacgacgca aatcggtcaa cagttttgag cgctgattgc
    54241 tctgaaccga aaatatttcc ggttcgaatt gctgcacaat atttttgggg gtgtcaattt
    54301 ttaaaaactc tccgttcaat atcaaagcaa tggtatcgca aagtccggct tcgtccatat
    54361 aagctgtaga aaccaaaata gttattcctt catttttgag attttgaagc atttcccaga
    54421 attcccttcg cgaaaccgga tcgacgccgg ttgttggttc atccaaaatc agaagcttgg
    54481 gtttgtgaat aagcgcacaa ctcaacgcca atttttgctt cattccaccg gaaagttggc
    54541 cggctcttcg gtttttaaaa ggttcgattt gccgataaat atttttaatt aaatcgtaat
    54601 tttcttctac tgtggttccg aaaacggaag caaaaaattt taaattttct tctaccgtca
    54661 aatcctgata aagcgaaaat cgaccaggca tgtaaccaac atattttctg atttcctgat
    54721 attcattcac cacatcataa ccaagcacag tcgcttttcc ttcgtcagcc aaaattagcg
    54781 ttgtgagaat acgaaacaat gtggtttttc ccgctccatc aggaccaata atcccaaata
    54841 tctgatgagg agatactttt aaatttatat tttttagcgc ctgaatatcg ggtggataag
    54901 tttttgtaat gttctcacaa caaaccgcaa attctccgtt atttcctgtc attctcgatt
    54961 ttctattaag gaaaatatca aaaattattg atcattgatt ttcggcacca aatttaacat
    55021 cagcatactg cccaattttc agcaaaccat cgctttttac gttaattttt acggcataaa
    55081 ccaaattggc tctttcgtcg cgcgtttgaa tggttttggg cgtaaattct gattcgccgg
    55141 aaatccaact gactgttcct tcatattctt tttgatcatc tttgccaaaa tcgacaaata
    55201 cttttgcttt ttg
//
LOCUS       DAOTAX010000005        50571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_562_length_50571_cov_31.278149, whole genome shotgun sequence.
ACCESSION   DAOTAX010000005 DAOTAX010000000
VERSION     DAOTAX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 50571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50571
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_50571_cov_31.278149"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLM04_01080"
     CDS             <1..217
                     /locus_tag="PLM04_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON01708.1"
                     /translation="LLNINMLYGCSEKVRKTGGLSTVSVIDTKGAVVKTIQNVGAESV
                     TFDVANKGLYMVRVQNSGKSATLKVVL"
     gene            338..3058
                     /locus_tag="PLM04_01085"
     CDS             338..3058
                     /locus_tag="PLM04_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON01709.1"
                     /translation="MKFFSRTCIFILFSFIHLTLFSAQLATWNLQGWRAQNSGGVRTA
                     QPTTTSTTVTVSDLWMASGIDPSNHLFTNNTLAGDKITAKTLAESKTNNEYYEFTIAP
                     ITGKVMTISSINICAMSQGQVCTFSLLSSLDGYTNPISTITTGSANNMNISQQTFDVT
                     GHNSLTNAVTFRIYIYSSDDVRWSIMGIGGHTSGISSDLIVNGSTTAPDLLPPTTPGS
                     LTALSVDATALRLTWTASTDDNEVVGYEIYNGSTLIGTTTNILFLNVMGLTPSTAYTF
                     SVKAKDSANKLSDPATVNVRTAQLGAGYVPTLPIGMNISSFNYYTPQTGFVDAMKMTG
                     DMFTYYKGGPWDSQQIGEIPRDDNGYPIQVPFTTSDGKASLIRFLLNNYYSGDYVLTF
                     DGTGTIEIGGLSNVSKVNNNKYYITFIGNGNNAWLNITSSTLGNHIRNMKILPKIYEN
                     AVTYPTFTAEFLDGLRPFHAFRFMDWICTNGSNESNWTNRVTKNYYSQGTSRGASYDY
                     AIELCNQLDADAWVTIPHKADDNYITQAAILWRDGLRPNRKVYLEFSNEIWNWQFSQT
                     SYINNNAPGHPNSYISSDLSAIQAAGGNFPEKDAYMMARCFRLWKAQFTGSNTNRLVR
                     VGTGQHGWADNTRRILNYLFDVDKNGCDMFSVAGYFNFQEKDHLVWNTKCSSVTPLEI
                     IDSVFADYPKYEGAWTDNTAAYVNARGIPYIVYEGGQHMQPYMQGEWCYNQAVYDAQI
                     YPKMYDLYMYNFEKMTSPIVNCNLFMAFSYVSPRESKWGSWGHLESLNQIGSASNYMT
                     IAPKYQALLDANAPKTAGLTTNPPYKSTNSNELNTYPNPVKNRLYFNFAENIENATIY
                     FYDIQGRIVKSATILDMQQYCADVTGLKSGIYLIKIISNGKLINRKFVKE"
     gene            3142..6960
                     /locus_tag="PLM04_01090"
     CDS             3142..6960
                     /locus_tag="PLM04_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON01710.1"
                     /translation="MKKLIITLSAMLLFICLSSSAEVTSFVENFNGPLPPYWQSTASY
                     SLSVKDGKLNMKVTKTSPWATFVVSLPETLNLSANPFVSIRTRVELPVRILLYLVDVN
                     NKSFPIHAKALYSENYYSNILFDFSGASGSIDLTQITKLYMGVNGDALTWTGKVWIDE
                     LKMGDVATKKATVGAVKNQTYYHGSKANELLITDVNNSNSILFENAESLVENVSIASV
                     GSGIYKLTFDAKANVSGTVNAKVISEATAGYVNDTVLFDLTVEQNLPPTAVFNTSIET
                     KTGVPLHFSITDITNGNINAIQKINFTLTKISGSSVNLISAVYDTIEARINVSADPIS
                     TGPTVYKLVLKDDGGGNDSLVSNIIINAYTKYNIAPEFSVPSSLEILLSQGNNEIPIL
                     DLSDGNNVVLQESLTFEVVSNNTTVVNNVVIKYIDGNPMKPAILFTPVAAGKAKLQIT
                     VSDSGEGYDNGPKQTVKTIDIIVKEDPITGYTIDLANFDAEVAAKKWTIEFEGTGQTV
                     TADNFGGQPCIKIVGTNKGQWNGLWYRTPELDLSKNPYISYEVYSVDNPIQTHVYFWD
                     NKGERNTPGAESERKTVPANVWTTVFMDFRGPSKLVNSSGIPINIQAIDTLLFNYNPQ
                     FTYPFTNWTGTIYIRNIKIGSDVPNVPALVPTVTVQSPGNSVFFKETGAQKVSLTGIS
                     CGTNCESVPTATIKSNSNPSLLSNVNLSAIHNDTIDLNFTVGTAIGNTTLTVEVAAEG
                     SITTSVSFTIYVVNNASSSTTFSIDTTTKKQVIAGFGADQPSINFIQQFKSTGATVVR
                     FFADGDAIEPLNDNSSATTLDRSKFLNDGLKIDYIKELKMNGINDIIITVLSPPAWMK
                     QNLSTSYATSAAQTWANTNNKVDPIYFDEYIEFMIAIVQLIKERTGVEIMAIAPQNEP
                     AFSQPYGSAILDPVNYAKICGMLGKRLTAMGYKTKVINPEQVFSQNFYPVTEYISAVR
                     ADADANKYTSVIGMHYPDNTVSTFRSQYAAASASPYPKEYWGVEMKAEGNDWSKVLTQ
                     MQTMVTALNNGVGLWAIYGWNGPPLLTNSINAVGSEIAMMYGSFTTRHFYAIKNIYNH
                     FRPSSCIVGSTMTPVNNNINMVTNLNEKDSIISVVWVNAGASNVVAELTMTYARAKFY
                     RTSEYEFYKEINPVSGNKILLPAKSVTTLSYSYATSSTTPNAISNVISDNNDMYSLYP
                     NPATNQINVVSKNACKFSLSIVDMMGRTILVKNGLDSKTPISISDLNQGIYNVIIKSD
                     KVYVKKLVVQ"
     gene            complement(7059..7946)
                     /locus_tag="PLM04_01095"
     CDS             complement(7059..7946)
                     /locus_tag="PLM04_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HON01711.1"
                     /translation="MENINAFREVTPLSSEDCFFIIDRIKSKMTYPVHYHPEYELNFI
                     ENGKGIIRIVGDSLEEIDDLELCLIGNEQLEHGWVEPDNREPELMHEITIQFHPDLFL
                     ESLLNKLQFQSIAVLLENAKKGVVFSREAIYSIKNRLKLLSEHESNFEAVIELFSILN
                     LLSHDNNYRILCNSSFVKNDDVSESRRVQKAINYIKMHYTEKLSLNDVANQVNMTTSS
                     FSRFMKSRTGKNFIEYLNDLRLGSAARSLVNTNKTISEICYECGYNNLSNFNRLFKQR
                     KGYTPKEFREKYKKMRKLI"
     gene            complement(8650..9117)
                     /locus_tag="PLM04_01100"
     CDS             complement(8650..9117)
                     /locus_tag="PLM04_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HON01712.1"
                     /translation="MIQRIQTFYLFLVLILSSVTLFSPVAVLFNKESATLYEISYKGL
                     CLLDESSKVPVSSVWALTAISAIVPVVTLIIIFLYNKRLIQIRLSIFNMIIMLGYYVI
                     LFIYLFYAAKTLHADWSLKIVSAFPLVNIILTLLAMRAIAKDEALIKSLNRLR"
     gene            complement(9184..10170)
                     /locus_tag="PLM04_01105"
     CDS             complement(9184..10170)
                     /locus_tag="PLM04_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HON01713.1"
                     /translation="MADNSLKKILKHYFKKIKTFFLSKDIFIFLLFFVFSAGLWFVNA
                     LGKERERIISIPLNYTGIPQHIAIVNNPPKSLSVKIKDEGMHLLQYRQKRLKPLDIYF
                     SQIFYEKGKILITPDQISNYLLRYLQPTTLVLETKPDSIVVEYEKLSQKLLPVECKIK
                     YSLAQQYMIVDKILLQPSKITVFGTAQQINSLKTLKTEPLLLSNLNDTVNTICRLQPI
                     HGVRFTTEAIQVFIPIEKFTEKKVRLPIQVLNVPNYITIRTFPTQVEATFNIALSRFS
                     SFDPAQIQLAVDYHQIKESASDKLQVFVIDHPSYVQNIRLSPQQVEFLVEKF"
     gene            complement(10180..10509)
                     /gene="yajC"
                     /locus_tag="PLM04_01110"
     CDS             complement(10180..10509)
                     /gene="yajC"
                     /locus_tag="PLM04_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HON01714.1"
                     /translation="MNLTAILLQATPAANGTGGGYTGILMMILIFVVFYFFLIRPQTK
                     RQKEIKKQRESMKVGDHVVTSGGIYGKIKEVKDNTMIIEIAENVRIKVDKNSVFLSPE
                     DIQSQAK"
     gene            complement(10586..11536)
                     /gene="nusB"
                     /locus_tag="PLM04_01115"
     CDS             complement(10586..11536)
                     /gene="nusB"
                     /locus_tag="PLM04_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HON01715.1"
                     /translation="MINRILIRIKIVQVLYSFYKNDEKSFTVAEKELFHSIEKTYDLY
                     FHLLQLAVEITRFANLRIDARKNKLRPSPEDLNPNMRFVENSFVEQLSTNKQFTKYLT
                     ERGLSWSDYPEVIKRLYEQITASDFYAEYMNAPFVDYEVDKNLWKKIYKKIILQSEEL
                     DSSLEEQNIYWINDIELVVSFIIKTIKKFQLSAGAQQELLPMFRDEEDVSFAAKLLKN
                     AISNCDEYRALIDKHTKNWELDRIAFMDIIIMQAALAELMEFPTIPVNVTLNEFIEIA
                     KIYSTEKSGTFINGILDNIVGQLKKENKLIKVVMFNENKK"
     gene            11803..12165
                     /locus_tag="PLM04_01120"
     CDS             11803..12165
                     /locus_tag="PLM04_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HON01716.1"
                     /translation="MEDVTRKEDLNKQPNEIVYSRAIKAGKRIYYLDVKKNKNDNFFL
                     AITESKKKVLGSDEDAQVSFEKHKIFLYRQDFEKFLEGLQDVMKFIRLQQPEEPYAES
                     HGKSSETTDEQDDFFLTK"
     gene            complement(12360..12542)
                     /locus_tag="PLM04_01125"
                     /pseudo
     CDS             complement(12360..12542)
                     /locus_tag="PLM04_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
     gene            complement(12623..13222)
                     /locus_tag="PLM04_01130"
     CDS             complement(12623..13222)
                     /locus_tag="PLM04_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01717.1"
                     /translation="MKKYIYLAFLCSFTLISCDENKEPAVTLKNEIKVKVFSTDTWDP
                     TTNKMDTVVGATVNLISDSGTSSVITDNKGIATFSNVTEDDYIIVAKKDNLCNLINTS
                     LEGDMTIGNLIIGVYTSEKDIEQSAFYANARVGGNKLADANGDGLINNLDKVRGQSSE
                     FEYQYKDLNGDGTIDAKDLSNGNLIQIDHQVEETIFIGK"
     gene            complement(13342..14601)
                     /locus_tag="PLM04_01135"
     CDS             complement(13342..14601)
                     /locus_tag="PLM04_01135"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HON01718.1"
                     /translation="MNATNHSYDWKEAALNAARTLQLVDDKAISETLLALADEIAIGS
                     EKILKANAEDLAAMEKAYPMYDRLLLNEQRLQNIAADIRNVAGLPSPLGKTLMETVRP
                     NGMKISKITVPFGVIGIVYEARPNVTFDVFSLCLKSGNSCVLKGGSDAYHSNAAIVSL
                     IREVLNRFGLNPDIITLLPSGHEATADLLNATGYVDLVIPRGGKGLIDFVRTHSKIPV
                     IETGAGICHTYFDEFGNTEKGSKIILNAKTRRVSVCNALDCLIIHEKRLSDLPALCKK
                     LAEKNVVIYADAKAYTFLVGKYPVHLLQKATQESFGTEFLDYKMAIKTVESLQEAIQH
                     INCYGSKHSECIVSENEANIALFDAMIDAACIYTNVSTAFTDGAQFGLGAEIGISTQK
                     MHARGPMALNELCTYKWIIKGNGQVRE"
     gene            complement(14671..15426)
                     /locus_tag="PLM04_01140"
     CDS             complement(14671..15426)
                     /locus_tag="PLM04_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON01719.1"
                     /translation="MPEFENVLENREFQTLPSVSVDCVIFGYEGNLLKVLVRREYVPF
                     EGNILIEWKLPGNHVRRNEQIDATASRILKEQTGLENIFMKQFMVFSDPDRLKRRERD
                     YEWVKPRLQDDRVLTVGFYSLVNIHDVDNSKLLEEAKWENAYEIRELMFDHNDIFNEA
                     LKKLRYDLLHEPLIFELLPEKFTLTQMQKVYEAIFDTTYDKRNFRRKVNKMPYLIQTD
                     ELQSGVSHKPARYYTFDREIYENTRSERFDFRM"
     gene            15663..17975
                     /locus_tag="PLM04_01145"
     CDS             15663..17975
                     /locus_tag="PLM04_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HON01720.1"
                     /translation="MKNKRLNLFYLLLFFIPIFSAISAQESVSDRVEELLSKMTIEEK
                     VGQMAQITLDAIGNGATVFESKIPFAIDSVRLHKAIVDYHVGSVINTTNNYALSALQW
                     NSIIDQIQKTATTKTTLRIPVIYGIDAIHGVTYTSGATLFPQQIAIAATFNPGNAYNM
                     AKVTAYETRASGIAWNFSPVLDLGADPRFSRQYEGFGEDPYLASVMGRQAIRGYEGIT
                     DSIGHAEKVAACAKHFLGYSVPFSGKDRTPAYIPDHILREYHLPSFKAAIDEGVESVM
                     INSGIINGVSVHANYNIITKLLKEELGFQGVVVSDWMDIINLEKRDKIAKNNKEAIAL
                     AINAGIDMSMVPYDYEQFCKDLTALVKEGKVKESRIDDAVRRILKLKFKLGLFENPVT
                     NLSNYPDFGSDKHEKLAYKAAADAITLLKNKNNILPLNKNIKILVTGPNANSMRSLNG
                     GWSYSWQGEKTERYAAQYHTILEAVQQKIGEKNVLYEPGVSYVETGQYFEEKENGLQK
                     AVAAARKVDAIVLCLGENSYAEKPGDLNDLNLSELQIKLAKSLSAVNKPIILVLNEGR
                     PRCFSAIESKMNAIVQTYLPGNYGGDALADILFGDVNPSGKLPYTYPAYPNSIVNYYH
                     KFSEEQKPSEGAYKYESDYNPQYEFGFGLSYTTFAYSNLKISSSVLYPNELIEVSVDV
                     TNTGTREGKETVLLFTSDVIASITPDMRRLRRFEKINLKPNETKTVKFTLSANDLAFV
                     NTDNQWTIEPGEFEIKIGNLKKTINYQSTN"
     gene            18091..21003
                     /locus_tag="PLM04_01150"
     CDS             18091..21003
                     /locus_tag="PLM04_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON01721.1"
                     /translation="MPLALSAQKTTITGVVVDSNNEPVIGATVLIKGTTTGTVTNYEG
                     KFTIEASSNDVLVVSYIGMVPQEIKVGNSKNINVVLKEDIKILSEVVVVGYGTQKKKD
                     LTTAVSTVSAEDFNVRPIVSAGQAIQGKAAGVQVIQPNGAPGGETVIRVRGTTSFNGN
                     SDPLYVVDGVPVDNINFLSPNDIENINILKDASSAAIYGSRAANGVVIITTKQGKSGE
                     SKIAFNAQLTAKNVTNKIESLNAADYKDLMEEVRPGSSSQMSSDDITNWYDEVYRTGL
                     QQSYQLSFSNSTEKTSYFLSAGYLDEKGIFPASFFKRYSFRANMTNEIRKWLSIGSNI
                     SYSDNSNNGITTGAGSNRGGVVLALINLPTSLPVINPETGYYNRSFYGQNITNPVEAM
                     ENGKDDITRENRLIASGNAMITFMKGMNFKSTFTLDRRHGLQTGFSPVIHVTGIDEYG
                     SAWDNRNMNTVLTWDNIFNYQKSFDKNNIELMMGSSWTDSKYTNSWINGSHYRNNKIK
                     TLNVANKISWNNTGTGGSTWGIMSYFGRVSYNYNSKYLITANFRADGSSRLHPDHRWG
                     YFPSFSAAWRISSEEFMQGLNWLDDLKIRGGWGQTGNQLGLGDYAYLQRYSVTRQQWW
                     ITGQENILPIITASNLRTTDLTWETTTQSNVGIDFTAFKSRLNIIMDYYYKKTTDMLL
                     NITLPSGANVTSIQRNGGLMRNTGFELSVNSINITNKNFKWTSDLEFSLNRNKLVKLD
                     LTKIYYDAATSDAFHGIQIVRNQEGRSLGGFYGYISDGVDSETGELIYRDINGDNKIT
                     ASDRTFIGDPNPDFIYGFTNTFTWKGFSLSIFLQGTYGNDIFNASRIETEGMYDLKNQ
                     STRVLDRWRRPGMITTIPKANFNLQPSSYFVEDGSYLRLKDLTLSYNVTAPFLKKSGI
                     SRLQPYITASNLLTFTKYLGMDPEVNEWGNSNAVQGIDWGTYPQAKSFVFGFNIEF"
     gene            21017..22567
                     /locus_tag="PLM04_01155"
     CDS             21017..22567
                     /locus_tag="PLM04_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HON01722.1"
                     /translation="MKIIKYILISAVVFVAASCSLDYNPIDTYSDITEGIDTTSAASQ
                     VILKDKAAVLNQRQVLYQRLKDGQEHWYLDMLLLAESHSDNAYAGSPNAETTPFEINS
                     IEGSNPNLKRDWNGHMDNIAQANRLIQGIDELKDPSLTEAEAKQIRAEGLIFRSLVYF
                     DMVRIWGRVPLITTVGKDITADNIEEVYPAYFPQQSEEIDVYRQIEEDLLEAVRYAPN
                     NDPSNKTLLTKSVARALLAKVYAEKPLRDYNKVIAYADSVAADGFELVSDFSDLWGVQ
                     LQNPSAPPGENNFAIDAKKRNTSEAILEIQYPVGSGNWVTWMFGRSLDNWNFWFDWAK
                     WITPSRDLINAFQAAGDIKRYNESVVWYSCSWNIYYPSNNYPFMYKTRSAYNSIIKIR
                     YADILLLKAEALIQTGDLSGAASIINDKIRNRAGLAPLPASVTNDKNALLSAYLNERR
                     LELAFEGQRWFDLVRLEKVEEVLNTLPSRDSGRPALRYPYDKYSYKLPIPQDIIDQNP
                     NLVQNPGY"
     gene            22587..24014
                     /locus_tag="PLM04_01160"
     CDS             22587..24014
                     /locus_tag="PLM04_01160"
                     /inference="COORDINATES: protein motif:HMM:NF027723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HON01723.1"
                     /translation="MKNIIKLTIFGLLGLFATSCQQEELRVHYPASKPVFDSISVPET
                     NIVYGDSITLYVGVSDPLTPLSTLEIKVVVNDELIASETIRTKGNQATYQQRYGIPFV
                     ARMPDNAEVEIHLTSINVEGTKTDSIYVNTIAKRPTITDLYLVVPLKSPVKMKLTDAQ
                     NYIYTADNLSLENTVSFRLATKLKYGISIDWTGLVFGKVGNGIGLIDINGSPITLSDA
                     TLIGYKKITVDLFNFTVKGEGDKLQPATAMDISTFGTVSLSSTDYLNVTTKEDWKTKK
                     MYLGKGTEMTISGIQTTNLANNLAPDFFEVTGSNTAKFLGETGIYTVYYLPRLNFVFV
                     EQPDAVYPDAMWLCGVGFGPARTPHIKTSSWNWNTPLEYQFCRKVSDGVYQATFYALH
                     QIDDTAAEPWRLKFSVKFFHQRMWGGEEDARTYTLNVNGLLFAPTESDQGNFAGTDAF
                     KDKPGIYRLTIDVNNKTANFQKISD"
     gene            24071..25519
                     /locus_tag="PLM04_01165"
     CDS             24071..25519
                     /locus_tag="PLM04_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HON01724.1"
                     /translation="MNKKSIFVFLFLLWVFIFSTGCKHSGDEPVPTPTPQDSVGDVSV
                     WLTTGNESCLLKKMTPINFSRDFADFTIVIDTTTTYQYIDGFGAALTGSSAHLIHQMS
                     PQNRKKLLDDLFDPEKGIGINYLRITIGSSDFSIGTYSYCDSPDINKFDIPEIDRNEL
                     LPVLKEILEINPSMKILASPWSAPAWMKSNNSMYGGSLKGEEVYNDFAEYFVRYVQVF
                     EQEGIHIDALTIQNEPMYETGGYPTMKMLWQEQNKIIRDYLGPKFIANGIQTKIVIWD
                     HNFDMSYYPINILNDPVTRQYVNGTGWHGYGGNASAIDQVTALYPDKETYFTEISGGG
                     WNTDTRMGNMFYYMKDFLMATVNRGSKNFLMWNLALNPQHGPTTTTWGGCQDCRGVVT
                     INENETYIRNEEYYLLGHFAKVVRLGAVRVNNSCYNLPSGMTISSFMNADGSKVVVIL
                     NRSGSSRKYNVRCGTRKFYYQQVDESVVSFKF"
     gene            25568..26941
                     /locus_tag="PLM04_01170"
     CDS             25568..26941
                     /locus_tag="PLM04_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01725.1"
                     /translation="MKIRALFFLMAFAIVTNCISAGKVTVWSSLPDTKALLVKKSMKL
                     NSKPVEIKNKVEIFPDQTCQSVEGVGAALTHSSAYVFHHNLTAQRRDSLFKVLFTPAG
                     IGINYTRLCIGASDFAFNLFSYSETEDFSLSNFNIQEDEKDVIPMLKEILAINPNLQI
                     LASPWSPPGWMKTSGSMVGGKLRPDCYDVYAEYLIKYIEAYRQHGITIHALTVQNEPE
                     YGTAAYPCMDMTAKEQQIFIRYFLGPKMQKKGIQTKIILFDHNCDNPDYPISILNDPD
                     TKKFVDGSGFHLYKGEISALCKVHEAHPDKNIYFTEQSGGGWAPDFDQNVRWYVGELF
                     AGAMNCWSKNVLLWNLALDENDGPKNFGCQNCWGVVRVTSDGRIEKNAEFYAIGHYGK
                     VVQPGAVRLVSNQDILKGVAFRNPDNSFAYLAVYYGTTPQTIGIQCGNSSFVYTFKPG
                     EVVSFLW"
     gene            complement(27266..27991)
                     /locus_tag="PLM04_01175"
     CDS             complement(27266..27991)
                     /locus_tag="PLM04_01175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HON01726.1"
                     /translation="MLTENVFYIPQTISTNVLCWKMFKERDIPEGLVVVADYQTAGKG
                     QAGNSWESARGKNLLFSIVLYPHHIPIEEQFLISQAVSVGIKNVLDTVSDDITIKWPN
                     DMYWKDSKLAGILIETSLFRGKISKAVIGVGLNVNQTEFFSDAPHPISLKQIAGKSFN
                     RKKLLHRIVASILEVYGQWESEKIRSVYAQSLYRKNGYHFYQAGTEIFRAKISRIYPD
                     GALELETEKGTSAKYYFKEVKFL"
     gene            complement(28002..29411)
                     /gene="dacB"
                     /locus_tag="PLM04_01180"
     CDS             complement(28002..29411)
                     /gene="dacB"
                     /locus_tag="PLM04_01180"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HON01727.1"
                     /translation="MKKIFFNVFVSFFVLNLVAQTPVDNFIHHPLLKNANVSLLIRET
                     GKGKVVGRYNANKSIIPASTLKLVTTASALELLGPNFRFQTKLQISGNVTKEHVLQGN
                     LIIKGAGDPTLGSEFLGEKNFLDQWVLAIKQAGISKIEGKIIADASIYNQEGVSPFWI
                     WEDIGNYYAPSIFGISYLDNTFRLVLRSGPIGTTPAIIRTLPDLPDLVIENYLKSSKI
                     TFDSAYFCGMPNSNLRRLYGEIPANRAEFVVKGDIPNPVLLLAKQFSDKLIQSGITVN
                     NPPVAEFSTPFDGQVVYTHFSPPLRDIIKQTNERSNNHYAECLFRYLGLGEGVPVTGR
                     GGVKTIRNLWKLKGLPVDQLFMCDGSGLSPMNAVSAQFITELLDYMYSKSAFSKDFFN
                     SLPIAGKTGTLVSFLAGTPLEGKVFAKSGSIEKVKCYAGYVKNEGKTYTFVVMVNNAQ
                     GTSRAVTKKIEELLLSVFK"
     gene            29862..32042
                     /locus_tag="PLM04_01185"
     CDS             29862..32042
                     /locus_tag="PLM04_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON01728.1"
                     /translation="MKNFTFLLLFSVFGGELLCHASGEQANIISLKNDSLLFSSDQIE
                     LDAFDSIPEKNIKEVVVYGISSGRLTFPLSLVSKTSLLNSSFTTPADALQRETGIFMS
                     RDGIWATSVNIRGLSEQRLLFLVDEDRLLTATDIAAALSTVDMNALERIEVIKGAGSV
                     LYGTGAMGGVVNFVIERPAFTPFLESQGNIHSSFNSSNQLWSNHAKLQLTNRQWYLSL
                     NGSYRTADNMRTPEGKIPNSQFNDASWGMQAGMSSVANQELLVNYQHFEAWNVGLPGG
                     SAFPPTATVRYKNVQRNLFDAEYVFYEITDYLKQLRLKAYTQNISRDVENIVNTSTAI
                     FPSSNNNTSGIKAVGDWEFTAYHKLTLGTEGWLRDAKTSRIKKVNSGTQINVFGEQPT
                     PNAQVQDMGIFAQYSWEFSPRYWMLDAGIRLDYMKTTNDSAFQPVYKYSYFPENPSMR
                     IYEDIPRSLLFKADEKNEFSYSAHIDLIYHPTRRQMLALSVSNSYRAPSLEELFKYID
                     QAGTLRVGNPDLKPEKGFFSNFSYTLSEDHFYIKTDLFANYLFDLIAEQHGTYTYQDE
                     KGKVTQREALINTNIDKALFLGGEMELQWKINPVFSVLANLSYTQGRNIDKDTFLPQI
                     PPMHGLFSVNYHQKELFDASVSALWAAKQGKVAPGENPTNGHLIFNADLHTEKFRINR
                     NYVRFFAGAENIFDKAYYNHLSTTRGVLRLEPGRNIYVKAELGW"
     gene            complement(32227..32811)
                     /locus_tag="PLM04_01190"
     CDS             complement(32227..32811)
                     /locus_tag="PLM04_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HON01729.1"
                     /translation="MKKNTIIIVIAALALFFLWGVSRYNKLVSAEEAVSSQWGNVENQ
                     YQRRADLIPNLVATVKGYAAHESQTLEAVTAARAKATQVTVDPASLTPEKLQEFQAAQ
                     GELSTALGRLLMITENYPDLKANQNFIELQAQLEGTENRIAVERNRFNEIAKNYNATI
                     RRFPTSLIAAMFGFEKKPYFEAQEGSEKAPEVKF"
     gene            complement(32924..34237)
                     /locus_tag="PLM04_01195"
     CDS             complement(32924..34237)
                     /locus_tag="PLM04_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HON01730.1"
                     /translation="MKKIFILLLTSFAICSIWSQTYTVETIPNPKKLNRFAFVSNPDG
                     ILNPETESQINNLLDSLEKKTGAEVAIVLVNSIGNEDIKSFAEQLFNSWKIGKEKEDN
                     GLLVLFVMDQRKVTFEVGYGLEGILPDAICKRIQIESMIPEFKKGNYDAGILAGVQRI
                     YATILKEPVQVEKTAPVNWKEILPLALGFYLIWLVLTWLWMGNAMNKVKINPRLNSNM
                     ARYIAAKNERNGIVSVMAIFLPIAAFIAILVLSQAIYLLLLLPLPFMTIPPLIYANYR
                     LQKIRKEPFPCSVCGGQMHFVPEKEEDVYLKLSQQFEEQLHAVDYDVFVCDNCKNEAI
                     FTIDRPSAYSECPKCGTKAFILKDKKLTMAPTYFNAGTERLTYRCEFCGYEENKNRRI
                     PRLTRANSTFIGGAAAGSIFSGSGGFGGGSFGGGMSGGGGATSGW"
     gene            complement(34266..34721)
                     /locus_tag="PLM04_01200"
     CDS             complement(34266..34721)
                     /locus_tag="PLM04_01200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON01731.1"
                     /translation="MKPKSFFLFISTLLIFSFAGCKENNYEDWKIVNDQWMLKHQSDS
                     GFVVTSSGLCYQIIHQSVGRKPNASSWITANYKGYLIDGTLIEKGTYDYYLSSSISGW
                     QEGITKMRDGSTFIFYIPSSLGYGKEGSGSKIPPYSALIYEVTLVSSYY"
     gene            complement(34834..36207)
                     /locus_tag="PLM04_01205"
     CDS             complement(34834..36207)
                     /locus_tag="PLM04_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HON01732.1"
                     /translation="MKPARKFHFDASKIAFDEVHRKKIDFNMSRYDLAVETGMKRYKD
                     VEAAKNQAAAIKHEVLSHLDEYLLQFEKNISSHGAEVLWARNSEEAIVYVEKILVEND
                     ARLLVKSKSMTTEEIELNAAAAKLGCESVETDLGEFIVQTAGEKPYHIVTPAMHKSKS
                     EIADLFHEKFDTPSDSTPEQLSDFVRRLLRKKFTQAEIGVTGANFLIADMGGISVTEN
                     EGNALMSTSFPKIHIVFAGIEKIIPKMEQLGLFYPLLAVHGTGQQMTAYNTIFTGPRQ
                     TGETDGPEKMYVILLDNGRIGIFADDEAYQSLACIRCGACLNGCPVYRTIGGYTYNTT
                     YSGPIGSAISPFLKGFRDFYHLSYASTLCGKCAEVCPVKIPLPDILLANRRKAVDQHL
                     SSALETAAMKGFRLIASKRKGFDFFPAGMKNSAFYPLNFWGWGPERTVPKFAKKSFSQ
                     LYRKNNK"
     gene            complement(36228..37115)
                     /locus_tag="PLM04_01210"
     CDS             complement(36228..37115)
                     /locus_tag="PLM04_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01733.1"
                     /translation="MKKNNLILIVLSVVVLLLIGTVIFFVQVNKQKDAEMSEVVEMMN
                     FEKEQLEKDYSDLALEYDTYSSTIRNDSLVQLLQNEKMKVQQLLQELRITKATNARRI
                     AELRNELATLRTVMAQYVHQIDSLSAANKVLRVENVEVKRKYEAASETVKQLSKEKEN
                     LTEVVTRASMLEISNFSMTPLNSKNRKTGRYSQITTLQFNYTIQKNITCPPGMKTLYL
                     RLTRPDGEVMTKDPDDVFLYENKYIAYSAKKDFEYAGEALSDVIYWKVDEILQVGTYR
                     ADFFVDGFRIGSFTFEIKK"
     gene            complement(37171..38475)
                     /gene="murF"
                     /locus_tag="PLM04_01215"
     CDS             complement(37171..38475)
                     /gene="murF"
                     /locus_tag="PLM04_01215"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HON01734.1"
                     /translation="MQLYDIFLQHPIITTDSRNCPENSLFFALKGENFDANAFAHSAL
                     EKGCAFAVVDEKEYAVDNRFILVDDVLKTLQQLAHFHRQQMDIPVLGITGTNGKTTTK
                     ELITAVLREKFNILSTQGNLNNHIGVPLTLLQLKPEHQLVVIEMGANHPGEIDQLCQI
                     AAPNYGLITNVGKAHLEGFGSFEGVMRTKGELYDYVFRHGKKIFIDKNNNFLREMAFS
                     AGFSTSERIIEYGLNIPNKEPAAISGEILENSVFLKMNCHTPEGTFIVETRLIGAYNA
                     ENILAAIAVGSFFGVENNAIQRGIENYIPQNNRSQLTITQKNKLIVDAYNANPSSMSA
                     AIKNFARMEGEPKTLIIGDMLELGKQSEEEHRNIILLLQQNGFKNVLLVGSQFQKVND
                     NYRTFQNVEDLIKYLESSPLQGQNILIKGSRGIHLEKVLPFL"
     gene            complement(38632..39264)
                     /locus_tag="PLM04_01220"
     CDS             complement(38632..39264)
                     /locus_tag="PLM04_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HON01735.1"
                     /translation="MKRVKFFVFTAILFAAVTLNAQSFGLEAGYGNSSTFVGEGDTLN
                     TLNGFHIGPTAELQIQGPLSLQYGLFYNFLTSSKTYENSLVDTKISTTFHSIDIPVRL
                     AFTVPAGNNVGVFIFGGPSFNVGISKKSTGKVTSSLGTIETTIDYYKTDDEGNSRQSR
                     FDIQMGVGAGIKVNGVQLRVGYDWGLLDTNNSENYTLTRDEFKASIGFDL"
     gene            complement(39405..40067)
                     /locus_tag="PLM04_01225"
     CDS             complement(39405..40067)
                     /locus_tag="PLM04_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HON01736.1"
                     /translation="MKKSLKPVFILTGILYFYLPLSAQTYRIEAGYLNQVRSGSGLSS
                     TYFDGAQVGVSSDFALKNNFSYSVGGYYSFLYSFKEQLFPHDTYVRYTTYSHSLDIPV
                     RLNYTLTIKKVKLFAYAGPNFNIGLYQPQKTVSTLTPELTELVQEFQHVTIPNDTTRS
                     ELYNDNVLRRLNIQLGAGGGIQWRKYRLKSGYDFGLLDLYKAKTASLHQSGWFVSLGY
                     EF"
     gene            complement(40189..41307)
                     /locus_tag="PLM04_01230"
     CDS             complement(40189..41307)
                     /locus_tag="PLM04_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HON01737.1"
                     /translation="MNLYPKLILETLSHVRYPGKGQDIVSLGMVQDNIRIEGNKVSFS
                     LIFEKPNDPFVKSLVKAAEQAVLTYVGKEVDIKGNITAVFKKEIPTAKENSILPGVSN
                     IIAVFSGKGGVGKSTIAANLAVALASLGYRVGLLDADIHGPSVPKMFGLEDARPFLEE
                     VDGKELIVPIEKYGIKLLSIGFFVDPDNALVWRGSMASNALKQLITDAHWGELDYFIM
                     DLPPGTGDIHLTLVQTMGITGAIVVTTPQEVALADARKGINMFTNEKVNVPVLGLVEN
                     MAWFTPAELPENKYYIFGKEGGKRLAEEFNIPLLGQIPIVQSIRESGDAGKPIATEED
                     SILSVSFRQLAENVVAAVEQRNKIMEKTKKVEINHPRK"
     gene            complement(41329..42090)
                     /gene="trmB"
                     /locus_tag="PLM04_01235"
     CDS             complement(41329..42090)
                     /gene="trmB"
                     /locus_tag="PLM04_01235"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HON01738.1"
                     /translation="MSKNKLAKFAEMETFPHVFQFPAKMLRNEHSNFEMQGKWNEKFF
                     GNSNPIVVELGCGKGEYTVGLAEIFPEKNFIGVDIKGARMWTGAKYSFEKKIPNVAFL
                     RTRIEMLHYFFGENEVSEIWLTFPDPQMKKITKRLTSSRFLKLYSRFTVENALIHLKT
                     DSNFLFTYTMELLKLNHFPIEFSTDNLYEIASDHPILSIKTFYEQQWIERGIPIKYIE
                     FKLEKRIEILEPEVEIEHDQYKSFGRRPKHETTKN"
     gene            complement(42108..42599)
                     /locus_tag="PLM04_01240"
     CDS             complement(42108..42599)
                     /locus_tag="PLM04_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HON01739.1"
                     /translation="MNKTKKITTTSKFVLLLSITCLMVSCNRNDVYLQYRSLPTKGWN
                     KDSVLTFDVPITDTTSLYNVYVNIRHRGEYPYQNLWFFLRQATPLMDTLQKDTIECYL
                     ADQRGKWLGSGVGSVYEMSILYRQKVSFPRAGIYRYQIIQGMRDSILAGINDVGIKIE
                     KVK"
     gene            complement(42565..43782)
                     /gene="ricT"
                     /locus_tag="PLM04_01245"
     CDS             complement(42565..43782)
                     /gene="ricT"
                     /locus_tag="PLM04_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HON01740.1"
                     /translation="MDYRLDIGGYQIKKKSCNRCGNLKLSVHDWMADLPESQKETDFV
                     EVQFKNTRKGYYLNSTKIPLEKGDIVAVESSPGHDIGEVTLVGKLVLLQMKKNNVHPE
                     KMEIRRIYRKAKDTDLQKYQEAKNREHATMIQARQIAERMNLKMKIGDVEFQGDGNKA
                     IFYYIADERVDFRELIKVFAETFHVRIEMKQIGARQEAGRIGGIGPCGRELCCASWMT
                     CFNSVATSSARCQDISLNPQKLAGQCSKLKCCMNYELDTYQEAMKEFPSKEIQLETLD
                     NTYYHFKTDVFKGQITYSTAQNFGANIITISVQRAKEVIAMNKKGQKPEKLEEISEET
                     SQPRVVQDFENVVGQESVTRFDERKRKNPRFKNKNKKQKNNSDSNGQTASENKIAKPV
                     KKNEQNKENNNNK"
     gene            complement(43886..45037)
                     /gene="holB"
                     /locus_tag="PLM04_01250"
     CDS             complement(43886..45037)
                     /gene="holB"
                     /locus_tag="PLM04_01250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HON01741.1"
                     /translation="MLFSQVIGQEEVKKRLIRTVKEQKIPHAQLFYGPEGIGKLPLAI
                     AYAQYICCEHPLENDACGVCPSCVQFGKLAHPDLHFVFPIIKSEKKKLLVCDDFMDSF
                     RQIFAERIYFSQNDWYQKISGEAKQGMIYTSEGDEIIRKVNLMTYESRYKVMLIWLPE
                     RMNDQCANKLLKILEEPPENTVFLLVSNSPEEIIATILSRTQQVKIPRLSNEEIAEAL
                     VSRYQISENEAANAAQISEGSFLKALALIDENNVNRENFEHFVNIMRLAWIIGNKGDY
                     ASLKKIRQWSEEMAAASVGREGQKKFLSYAQRMIRENFIFNLHQPELNFMTSFEKEFS
                     VGFAPFINETNAEDMMNEFALAERHIEQNVNSKMVFFDLALKITKLLKK"
     gene            45566..48010
                     /locus_tag="PLM04_01255"
     CDS             45566..48010
                     /locus_tag="PLM04_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HON01742.1"
                     /translation="MAPKKPVKNGGRTTSGKVQKKSEKKSSGSGFGKKIASFFTDERT
                     HFIVGFMLLLVMLYLMLAFVSYFFTGAADQSKIDLSWSELHDKRTLIQNWTSVTGAIA
                     AEHLINRCFGVSAFFIPIFGIWVALRLMKAHEKSVWKAFFHCAFWLVWLSVTLGLTLS
                     PYFSDVLFFSLAGGHGDYAAQWLVSYIGASGSFLLMGGSFIVYLVIASASSLPFFRKL
                     FGKKNVSHTSFEETLVEPAVAVGTEIVPEEWISSGIETEVYEEFSKTQEESQTEKVAF
                     EVETPENNEESKPVDLDGNGENESLQEDPYYNLAKLGKYDPTLDLSHYHFPTLDLLKV
                     YGTDNETPIDMAEQIANKNRIINTLLNFDIEIETIKATVGPTITLYEIVPKAGVRISK
                     IRNLESDIMLSLAATGIRIIAPIPGKGTIGIEVPNNDPQIVSMHSIIASKKFQESKFE
                     LPIAFGRTITNEIFMVDLVKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSELKLVL
                     VDPKKVEFNIYAGIEKHFLAKLPDGSEAIITDTSKVVETLNSLCGEMDMRYDLLKKAH
                     ARNIKEYNEKFILRHLNPEKGHRFLPYIVVIIDEFGDLIMTAGKEVEMPIARIAQLAR
                     AVGIHMIIATQRPSVNIITGIIKANFPARVAFRVSSMMDSRTILDAPGANQLVGRGDM
                     LFSQGNDLIRVQCAFVDTPEVENIVHFISEQQSYPTAFYLPEYVKPEGEMGVASADLS
                     KRDPLFEEAARLIVANQQGSTSLIQRKFSIGYNRAGRIVDQLEIAGIIGPNEGSKARQ
                     VLIPDEYHLEQILKQL"
     gene            48185..48799
                     /locus_tag="PLM04_01260"
     CDS             48185..48799
                     /locus_tag="PLM04_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HON01743.1"
                     /translation="MLLSIISIQPALSQNDPEAQRIIDSFLNTARTGAITTRFTVSVI
                     EKNGVNSYTNSGTFTLKGNRFVLEMQEMKVWFDGKTQWAYQKSGNEVTITEPSEQELA
                     QTNPMAILGNYRNKCNIRFCKTSSEYWCIQLTPKSIKNEDIAEIEVQFLKTDGIPQSI
                     TVSQKNGTRTVLMLSQFQKNAKVSEQIFTFDAKKYKDAYINDLR"
     gene            48826..49770
                     /gene="trxB"
                     /locus_tag="PLM04_01265"
     CDS             48826..49770
                     /gene="trxB"
                     /locus_tag="PLM04_01265"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HON01744.1"
                     /translation="MTTNEKIRCLIIGSGPAGYTAAIYASRANLSPVLYQGMEPGGQL
                     TTTNEVENFPGFPEGITGPELMDKMQKQAERFGADIRFGMVTSTDLSASPYRVTIDNE
                     KIIEAQSIIIATGATAKYLGLPDEQKYAGSGVSACATCDGFFYRNRPVAVVGGGDTAC
                     EEALYLANIASKVYMIVRKPFLRASKIMQERVLQHPKIEVLFEHETLGLTGEKVVEGA
                     DLVKRRGQPDEEKVHIAIDGFFLAIGHKPNSEVFKPWIETDENGYIKTIPGTPRTNVE
                     GVFAAGDVADPHYRQAVSAAGSGCQAAIEAERYLVDKK"
     gene            complement(50383..50529)
                     /locus_tag="PLM04_01270"
                     /pseudo
     CDS             complement(50383..50529)
                     /locus_tag="PLM04_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT        15421 a         9674 c         9708 g        15768 t
ORIGIN      
        1 attattgaat atcaatatgt tatatgggtg ttcagaaaaa gtgcggaaaa caggaggctt
       61 gtcaactgtt tcggttatcg acaccaaagg tgccgttgtg aaaaccatcc aaaatgttgg
      121 tgctgaatct gtaacttttg atgtagcaaa caaaggatta tacatggttc gtgtacagaa
      181 ttctggaaaa tcagccacct taaaagtagt attgtaattt agaaacatat ttttatctta
      241 ttattttgta aaagttaaca attttcactg cgagaggtta tggtagtaaa attacctaac
      301 ctctcttttt aaaatatttc aaatgaaaaa tctaaccatg aagttttttt ctcgcacatg
      361 tatctttatt ttattctcat tcatacattt aacgctgttt tcagcccaat tggcaacatg
      421 gaatcttcag ggttggcgtg cacaaaactc aggaggtgta cgaacagccc aaccaactac
      481 cacttcaact actgtaactg tttccgattt atggatggca tcgggaatag atcccagcaa
      541 tcatcttttt accaataata ccttagccgg cgataagata acagctaaaa ctctggccga
      601 gtccaaaaca aacaacgaat actacgaatt tacaatagct cctattactg gaaaagtaat
      661 gactatttct tccataaata tttgtgcgat gtcgcagggg caggtctgta cattcagctt
      721 attatcaagc ctcgatggtt acacaaatcc gatttcgact attaccacag gttcggcaaa
      781 caatatgaat atatcgcaac agacctttga tgtaacaggt cacaattcat taaccaacgc
      841 agttacattt cgcatttata tatacagttc agatgatgtt cgctggagca taatgggtat
      901 cggaggtcac acctcaggca tatcatccga tttaattgta aatggttcaa ctactgctcc
      961 tgaccttctt cctcctacca ctcccggttc gttgacagcc ctgtcggtag acgcaaccgc
     1021 acttcgacta acatggacag catcgaccga cgataatgaa gtagttggtt acgaaatata
     1081 caacggatca accctgattg gtactactac caatatttta ttcttgaatg tcatgggatt
     1141 gactccttca acagcctata cgttttcggt aaaagcgaag gacagtgcta ataaattatc
     1201 agatcccgca acagtaaatg tgagaacagc acagctgggt gcaggctatg ttcctacgtt
     1261 acccattggt atgaatattt ccagcttcaa ctattatact cctcaaacag gttttgttga
     1321 tgctatgaaa atgacaggag atatgtttac atattacaag ggtggaccat gggattcaca
     1381 acaaattggc gaaatcccga gagatgataa tggttaccct attcaggtgc cttttaccac
     1441 ctctgatggc aaagcaagtt taattcgttt tttgttgaac aattattatt caggcgatta
     1501 tgtgcttact tttgatggca ccggcaccat tgaaattggt ggactttcca atgtttccaa
     1561 agtaaataac aacaagtatt atataacttt tatcggtaac ggcaataatg cgtggttgaa
     1621 tatcacatcg agcacacttg gtaatcacat ccgaaacatg aaaatactgc caaaaattta
     1681 cgaaaacgca gttacctatc caacatttac tgctgaattt ttagatggat tacggccatt
     1741 tcatgcattt cgctttatgg actggatttg cactaacgga tcgaatgaaa gcaactggac
     1801 taacagggtt acaaaaaatt attattcgca gggaacaagt aggggagcta gttacgatta
     1861 tgctatcgag ctttgcaacc aattagatgc cgatgcatgg gtaactatac ctcataaagc
     1921 tgacgataat tatattacac aggctgctat tctatggcgc gatggtttac gtcccaaccg
     1981 aaaagtttat ctggaatttt caaacgaaat ctggaactgg cagttttccc aaacaagcta
     2041 tatcaacaac aatgctccgg gtcatcccaa ttcatacatc agttcggatc tctctgccat
     2101 ccaggcagca ggtggtaatt ttccggaaaa agacgcttac atgatggcac gttgctttcg
     2161 attgtggaaa gcccagttta caggatctaa tacgaatcga ttggtaaggg ttggaacagg
     2221 acaacatggc tgggcagaca atacacgccg tattctcaac tacttattcg atgtagataa
     2281 aaatggttgc gatatgtttt cggtggccgg gtactttaat tttcaggaaa aagaccatct
     2341 tgtttggaat accaaatgtt caagtgttac accactcgaa attatcgact ctgtatttgc
     2401 cgattatcca aaatatgaag gcgcatggac agataatacg gctgcctatg taaatgctcg
     2461 tggcataccc tacattgttt acgaaggcgg acagcacatg caaccataca tgcagggcga
     2521 atggtgttat aatcaggctg tttacgatgc ccagatttat cccaaaatgt acgatttata
     2581 catgtataat ttcgaaaaaa tgacttctcc aatcgtgaat tgtaacttat ttatggcgtt
     2641 ttcgtatgta agtccgcgcg aaagcaaatg gggatcgtgg ggacatcttg aaagcctgaa
     2701 ccaaataggt agtgcgagta attatatgac tattgcaccc aaatatcagg cgcttttaga
     2761 tgccaacgct ccaaaaactg ccggtttaac taccaacccg ccatacaaaa gcaccaactc
     2821 aaatgagtta aatacttatc ccaatcccgt aaaaaatagg ctgtacttta actttgccga
     2881 aaatattgaa aatgccacta tttactttta tgacatacag ggacgcatag tcaaaagtgc
     2941 tactatctta gatatgcaac agtattgtgc ggatgttacc ggtttgaaaa gcggaatata
     3001 tctcataaaa attatcagta acggaaagct tataaacaga aaatttgtaa aggaataagc
     3061 tgttaatcaa acagttttag ttcttcagct ttttattttt taataatcaa ctatttaata
     3121 taacacccat ttattttacg tatgaaaaaa ttaataataa ctttaagtgc aatgttgcta
     3181 tttatctgcc tgtcttccag tgcagaagta acatcgtttg tcgaaaattt taatggtcca
     3241 ttgcctcctt actggcaatc cactgcgtca tattctttgt cggtcaagga tggtaaactg
     3301 aatatgaaag ttacaaaaac aagcccatgg gctactttcg tggtatcgtt acctgaaacg
     3361 cttaatttat cagccaatcc atttgtaagc attagaacac gtgttgaact acctgtacgc
     3421 attttgcttt atcttgtgga tgttaacaat aaaagcttcc cgatccacgc taaggcgttg
     3481 tattcggaaa attattacag taacatattg ttcgacttta gcggagcttc aggcagtatt
     3541 gatttaactc aaattacaaa actctatatg ggtgtaaatg gcgatgcctt aacttggacg
     3601 ggtaaggtat ggattgatga attgaaaatg ggcgatgtcg ctacaaaaaa agctactgtt
     3661 ggtgctgtga aaaatcaaac ttattaccac ggaagcaaag ccaacgaatt gttaataacc
     3721 gatgtaaata attcaaactc gattttgttt gaaaatgctg aatctttagt agagaatgtc
     3781 tctatagcat ctgttggctc cgggatttat aaacttacct tcgatgctaa ggccaacgta
     3841 tcgggcacag taaatgcaaa ggttatttcg gaagcaactg caggttatgt gaacgatacg
     3901 gttttatttg atcttacagt agaacagaat cttccgccta cagcagtatt taacacctct
     3961 attgaaacaa aaacaggtgt gccattacat ttcagtatta ccgatatcac caatgggaac
     4021 atcaatgcaa ttcagaaaat aaattttact ctcacaaaaa tttccggatc ttcagttaac
     4081 ttgatttcgg cagtttacga tacgattgaa gctagaatta atgtgtctgc cgatcccatt
     4141 tcaaccggac ctacggtata taaattagtt ttaaaagacg atgggggtgg aaatgattca
     4201 ttggtttcta acatcataat taatgcttac acaaaataca atatagcacc cgaattcagt
     4261 gtgccgtctt cattggaaat attattaagt caggggaata acgaaatacc tatacttgat
     4321 ctgagtgatg gaaataatgt tgttttacag gaatcattga ctttcgaggt agtaagtaat
     4381 aatacaacag ttgtaaacaa tgttgtgata aaatacattg atggtaaccc aatgaaacca
     4441 gctatcttat ttacgcctgt tgcagccggt aaagcaaaat tacaaatcac ggtttccgat
     4501 tcgggcgaag gttacgataa tggtcccaag caaaccgtaa aaaccattga tatcattgtt
     4561 aaagaagatc ctattacggg atataccata gatttagcta atttcgatgc cgaagtagcg
     4621 gctaaaaaat ggacaattga gtttgaaggt acaggacaaa cagtaactgc cgataatttt
     4681 ggaggtcagc cttgtataaa aattgttggg acaaacaaag gtcagtggaa tggattgtgg
     4741 tatcgtactc ctgaacttga tttatcaaaa aatccctaca tcagctacga agtttattcg
     4801 gtggataatc cgatacaaac gcatgtttat ttttgggata ataaaggtga gcgaaacact
     4861 cccggtgctg aatcggaacg aaaaacagtg ccggcaaatg tatggacaac cgtgtttatg
     4921 gatttcagag gaccatccaa gttagttaat tcatcaggaa ttcctattaa tattcaggca
     4981 attgatacac tattgttcaa ttacaatcct caattcacat atccgtttac caactggaca
     5041 ggcaccatat atattcgtaa cataaaaatt ggaagcgatg tgcccaatgt tcctgcactt
     5101 gttccgacag ttacagttca aagtccggga aattcagttt ttttcaaaga aactggtgct
     5161 caaaaggtaa gtttaacagg tataagttgc ggaacaaatt gcgagtcggt accaactgca
     5221 actattaaat caaattccaa tccttccttg ttatcgaatg taaatctttc ggccatacac
     5281 aacgacacga tcgacctgaa ctttacggta ggtacagcca ttggaaatac aactttaacg
     5341 gttgaagtgg cggctgaagg ctctataaca acttctgtgt cattcactat ttatgttgtg
     5401 aacaatgcat ccagttcaac aactttctcc attgacacaa ccaccaaaaa acaggttatt
     5461 gcaggctttg gtgccgacca accttcaatt aattttattc agcaatttaa atcaacagga
     5521 gctactgtgg tacgcttttt tgctgatggt gatgctattg aaccacttaa tgacaacagt
     5581 tcagctacaa ccctcgacag atcgaaattc ctgaatgacg gtttgaagat tgattacatc
     5641 aaagaactta aaatgaatgg aataaatgat attattatta ctgtgctatc tcctcctgca
     5701 tggatgaagc aaaatttaag cacatcttac gcaacatcag cagctcagac ttgggcaaat
     5761 acaaacaaca aagtagatcc tatatatttc gatgaataca ttgagtttat gatagcaata
     5821 gtacagctta tcaaggaacg taccggcgtt gaaattatgg ctattgcccc tcaaaatgag
     5881 cctgccttca gtcagcctta tggttcagcc attctggatc cggttaacta tgccaaaatt
     5941 tgtggtatgc tgggtaaaag attaacagct atgggttaca aaaccaaagt tattaatccc
     6001 gaacaagtgt tttcgcaaaa tttttatccc gtaaccgaat acatttcggc ggtaagagcc
     6061 gatgccgatg caaataaata tacttcagtt atcggaatgc attatcccga caataccgtt
     6121 tcaacatttc gatctcaata tgccgcagca agtgcatcgc cctatccaaa agaatattgg
     6181 ggtgtggaaa tgaaagctga aggtaacgac tggagcaaag tattaactca aatgcaaaca
     6241 atggttacgg cactcaacaa tggtgtaggg ttatgggcta tatatggttg gaatggtcct
     6301 ccgcttctta caaacagtat caatgccgta ggatcagaaa tcgctatgat gtatggcagt
     6361 tttactacca ggcattttta tgcaatcaaa aacatctaca atcattttcg tccaagttca
     6421 tgtattgttg gttcaaccat gacacctgtc aacaataaca ttaatatggt tacgaacttg
     6481 aacgaaaaag attcgattat atctgttgtt tgggtgaatg ctggtgcatc caacgttgtt
     6541 gcagagctga caatgactta tgcaagggct aagttttatc gtacttcaga atacgagttt
     6601 tacaaagaaa ttaatccggt ttcaggaaat aaaattttat tacctgctaa atctgtaaca
     6661 acactttcgt attcctatgc tacatcatca acaactccca atgcaatttc caatgtaatt
     6721 tcggataata atgatatgta tagtttgtac ccaaaccccg caacaaatca aataaacgtg
     6781 gtatcgaaga atgcttgtaa attttcgctt tctattgtgg acatgatggg acgtacaata
     6841 cttgttaaga atggtttaga tagtaaaaca ccaattagca tatccgacct aaatcagggt
     6901 atctataatg taattattaa atcggacaaa gtatatgtga aaaaattagt agttcagtaa
     6961 aaaatcagac ataaagcaac tgatttgtat ataaagaata acacctgaga agtttaataa
     7021 actcgtcagg tgtttttatt ttaataaaca cggcatggtt aaatcagttt tcgcattttc
     7081 ttgtattttt ctctgaactc ttttggggtg tacccttttc gttgtttaaa taatcggtta
     7141 aagttcgaaa ggttgttgta gccacactcg taacaaatct cagatattgt tttgtttgta
     7201 tttactaaac ttcgggctgc tgatccgagt cgcaaatcat tcaggtattc aataaaattt
     7261 ttaccggtgc gcgatttcat aaacctacta aacgatgaag ttgtcatatt tacctgattg
     7321 gctacatcgt ttaaacttaa tttttctgta taatgcattt taatgtaatt gatggccttc
     7381 tgaactctgc gactctctga aacatcgtcg ttcttgacaa aagatgaatt gcaaagaata
     7441 cggtaattat tgtcatgaga aagtaaatta agtattgaaa aaagctcaat aactgcttca
     7501 aaattagatt cgtgttccga taaaagttta agtcggtttt taatactgta aatggcctct
     7561 ctcgaaaaca ccactccttt ttttgcattt tcaagcagca cagctatgct ttgaaactgt
     7621 agtttattaa gcaaactttc taaaaacaaa tcgggatgaa actgtattgt aatttcatgc
     7681 atcaactcag gttctcgatt atccggttcc acccagccat gctccagttg ttcgttacca
     7741 atcaggcaaa gttccaaatc gtcaatttcc tccagcgaat cgcctactat gcgtattatc
     7801 cctttgccat tttctataaa atttaactca tattcaggat gataatgcac cggatatgtc
     7861 attttagact ttattcggtc gattatgaaa aagcaatctt cgctcgataa aggggttact
     7921 tcgcgaaaag cattaatatt ttccataata aaactatttt gaaaacaaat aaaagtaact
     7981 tactttatag caaaagaatt tgatttatta ctacttctgt tactttaaac aagtaaagat
     8041 acaaaaatca aacattaaaa tacattgttg tatataaatt aatatatatc aaagaatatt
     8101 aacattacat tttgtgtgat tgctgaatca aatcagaaac gcaacttttt aattaaacga
     8161 atttcagaaa aaatcaagtg gagttaaaaa tttatctaag tacatgaata aatttaaaat
     8221 tatggatatt taactaaaaa ctaaaattta aaatccgtgt tgcaatgaaa agaagatcat
     8281 atttaatatg acttttttcc gcttactgat ggtggcagag gaagtatgaa aaattagacc
     8341 caattgaaaa tttgtaaaat aactttcttt aatttacttt ctaatgtgaa catttttacg
     8401 gcattcgaat gataaacctg ttgcataatt ttttggttta attttgaata aattaatcaa
     8461 gtttttcatt cgtaggttct tttttaaaat tatgttgccg gttaacggag cgtgctttgg
     8521 agtatgtaca acgcaatttc gtaacccacc gatactgcta aattcacact tctatataga
     8581 acttagctct caacttttta atttacacac ttcgtgaaat agtgtccaat ttcctgtctt
     8641 tacacaattt catctgagcc tattaagtga tttaattaaa gcttcatctt tggctatagc
     8701 cctcatggcc aacagggtaa gaatgatgtt taccaatgga aatgccgaaa caatttttaa
     8761 actccagtcg gcatgaagtg ttttggctgc ataaaataaa taaataaaca gtattacata
     8821 ataacccagc atgatgatca tattgaaaat agaaaggcgt atttgaatca atcgtttgtt
     8881 gtaaagaaag atgataatca gggttaccac cggaactatt gcactaatgg cagttaatgc
     8941 ccaaaccgaa gaaacaggta cttttgaact ttcatccaat aaacacaaac ccttatagct
     9001 gatttcgtaa agtgtggcag attccttgtt aaagagcact gcaacaggcg aaaaaagagt
     9061 aacggaagaa agaattagca ccaaaaacaa ataaaaagtt tgaatgcgtt gaatcataaa
     9121 tttcgattta agtggttaag aaaagtaaat ttacgatgat aaaaacatga aaataaaaaa
     9181 atattaaaat ttttcaacca aaaactctac ttgctgagga gaaagtcgaa tattttggac
     9241 atatgatgga tgatcaatga caaaaacctg taatttgtcg gaagctgact ctttaatttg
     9301 atgataatct acagccaatt gaatttgggc aggatcaaag gaactgaacc tgcttaaggc
     9361 gatattaaaa gtagcttcca cttgagtagg gaaagtacgt attgttatat aatttggtac
     9421 attcaatact tggataggca aacggacttt tttttcggta aacttttcta tcgggataaa
     9481 aacctgaatg gcttccgttg taaaacgaac cccatgaatg ggttgaagtc gacaaattgt
     9541 atttacagta tcattgagat ttgaaagaag aagaggctct gtttttaacg ttttcaagct
     9601 gttgatctgc tgcgcagtcc caaaaacggt tattttgctt ggttgaagaa gaattttatc
     9661 gactatcatg tattgttgtg ccaaagaata tttgattttg cattctacag gcaacaactt
     9721 ctgactaagc ttttcgtatt ccacaacaat agagtcgggt ttggtttcga gcaccagcgt
     9781 agtaggctga agatagcgta aaagatagtt actgatttga tcgggagtga tcaaaatttt
     9841 tcctttctcg taaaaaattt gagaaaaata aatgtcaagg ggcttcaatc tcttttgccg
     9901 atattgcaaa aggtgcatcc cttcatcctt aatttttact gacaaagatt tgggtggatt
     9961 gttaacgata gctatatgtt ggggaatgcc ggtatagttc aatggaattg aaataattct
    10021 ttcacgttct tttcccaaag catttacaaa ccaaagtcct gccgaaaaca caaaaaaaag
    10081 caaaaagata aaaatatctt ttgacagaaa gaatgttttt atctttttga agtaatgttt
    10141 gagtattttt tttaagctgt tgtcagccat aataccctct tattttgctt gcgattgaat
    10201 atcttcagga cttaggaaca cggaattttt atctactttg atacgaacat tttcagctat
    10261 ttcgattatc attgtattgt cctttacttc cttgattttt ccgtaaatgc cacctgaagt
    10321 aaccacgtga tcacctactt tcatcgattc gcgctgtttc ttgatttctt tttggcgttt
    10381 agtctgtggg cgaatcagga agaagtaaaa caccacaaaa attaaaatca tcattaaaat
    10441 tccggtatat cctccgcctg ttccgttggc tgcaggagtt gcctgcaata aaattgctgt
    10501 taaattcata gatctataat ttatttgtta tttaatattt gatatggtaa agtgtttctt
    10561 ttttaatgag ttgcaaatat aagtattatt ttttgttttc attaaacatt accactttta
    10621 ttagtttatt ttcttttttt aattgtccaa caatattatc caaaatacca ttgataaaag
    10681 taccactttt ttcggtactg taaatttttg caatttcaat aaattcattt agagtaacat
    10741 ttacaggaat agtaggaaat tccatcaatt cagctaatgc agcctgcata ataatgatat
    10801 ccataaaggc aatcctgtcc aattcccagt ttttggtatg tttgtcgatc agcgccctgt
    10861 actcatcaca attgctgatg gcatttttta aaagtttggc agcaaacgat acatcttctt
    10921 cgtctctaaa catgggtaaa agttcctgct gagcacctgc cgaaagttga aactttttaa
    10981 tggttttaat gataaaactg acaaccaatt ctatgtcgtt tatccagtaa atattttgtt
    11041 cttccagcga gctgtccaat tcttcacttt gaagaataat ttttttataa atttttttcc
    11101 agagattttt gtcaacttca taatcaacaa agggagcatt catgtactcg gcataaaaat
    11161 cggatgccgt aatctgctcg taaagccttt ttattacttc cggataatcc gaccaggata
    11221 gtccgcgttc agtcaaatat ttggtaaact gcttgtttgt agataattgt tctacaaaac
    11281 tattttcaac aaaacgcatg ttgggattca aatcttcggg tgaaggacgc aatttgttct
    11341 ttcgggcatc tatgcgtaaa ttggcaaacc gtgtgatttc tacagccaac tgtagcagat
    11401 gaaaataaag atcgtaggtt ttttcaatac tgtgaaaaag ttctttttca gctacagtga
    11461 acgatttttc gtcgtttttg taaaaagaat ataaaacttg aacaatctta atccgaatta
    11521 aaatacgatt aatcataaaa tcagtggacg cttataaata acgcacaaag ataagtattt
    11581 tttttgttcc ctcaactttt gctctgcagc aaaaaatcga tcaactattt cagaaaaaaa
    11641 ccaaaatatg tccctattta gagaaaatga gaaatttatt tgaataatta aaaaaaaata
    11701 ctgaaatttg gagaaaattg acttcaacta taaaatttta ttataaattt ttatagttat
    11761 ttgaaataca gtgttttaat ttaaaaagag aggttgaaaa ttatggaaga cgtaacaaga
    11821 aaagaagatt tgaataaaca acccaacgaa attgtttatt cacgagccat aaaagctgga
    11881 aagagaatct actaccttga tgttaagaaa aacaaaaacg acaacttttt tttggccatt
    11941 accgaaagca aaaaaaaggt tcttggcagt gatgaggatg ctcaggtatc attcgagaaa
    12001 cataaaattt ttctttaccg tcaggatttt gaaaaatttc ttgagggact gcaagatgta
    12061 atgaaattta ttcgtcttca gcaacctgaa gaaccttatg ctgaatcaca tggaaagtct
    12121 tctgaaacca cagacgaaca ggatgatttt tttttgacaa aataaatttt taggaaaaaa
    12181 caaagagaaa attcaatagg aggatagctt tttcgttgtc ctttttactc gattaatttt
    12241 tacaagtaag agaaggtaca tttatgaaaa taaccatgtg attaaacttt ttccgttata
    12301 tgttattttg caatcaaata aattaggaag ttaatgtaat ttcactcttc cggatatttt
    12361 tattctctta cttgcccatt ccctttgatg atccatttgt aggtacaaag ttcgtttaac
    12421 gccattggac cgcgagcgtg cattttctgc gtactgatgc ctatttcagc acccaatccg
    12481 aactgagcgc catcggtaaa agcggtagaa acattggtat aaatgcaggc tgcatcgatc
    12541 attgcatcga ataaaaaaga gagattgctt tacgcgttta aatcttagtg cctttttgtc
    12601 agaaggctgc cgaagattac ttttattttc ctataaaaat tgtttcttca acttgatgat
    12661 cgatttggat cagattgccg ttagataaat ctttcgcatc aattgttcca tctccattca
    12721 aatctttata ttgatattca aattccgaag attgacctcg cactttatcg agattgttaa
    12781 tcaatccatc tccattggca tcggcaagtt tatttcctcc cactcttgca tttgcgtaaa
    12841 atgctgattg ttcaatgtct ttttcagaag tataaacacc aataatcaaa tttccaatag
    12901 tcatgtctcc ttccaaagaa gtattgatca gattgcacaa attatctttc ttagccacaa
    12961 ttatatagtc atcttctgta acattagaaa aagttgcaat tcctttatta tctgtaatta
    13021 cagaagatgt tccagagtcc gatattaaat tgacagttgc gccgacaacc gtgtccattt
    13081 tgtttgtggt aggatcccat gtgtctgtgc tgaagacttt tactttaatt tcgtttttca
    13141 aggttactgc cggttctttg ttttcatcgc agcttatcaa ggtaaatgag caaaggaatg
    13201 caagataaat gtactttttc atttttttag attgttattg taaattaact tttaaatatt
    13261 ttgacaaaga taattataag aacatgaaat aaatacattt caggaacata aattttcttt
    13321 ttgattaatt tttttaaaaa tttattctct tacttgccca ttccctttga tgatccattt
    13381 gtaggtacaa agttcgttta acgccattgg accgcgagcg tgcattttct gcgtactgat
    13441 gcctatttca gcacccaatc cgaactgagc gccatcggta aaagcggtag aaacattggt
    13501 ataaatgcag gctgcatcga tcattgcatc gaataaggca atatttgctt cgttttcgct
    13561 gacaatacat tcactgtgtt ttgagccata acagttgatg tgttgaatgg cttcctgaag
    13621 ggactcaaca gttttgatgg ccattttgta gtcaaggaac tcggtgccga aactttcttg
    13681 tgtagctttt tgaagcaaat gcacgggata ttttcctaca agaaaagtat aggctttggc
    13741 gtctgcatat ataactacat ttttttctgc cagtttttta cataacgctg ggaggtcgct
    13801 caatcgcttt tcgtgaataa tcaaacaatc gagcgcattg cagacactta ctcgtcgggt
    13861 tttagcattg agaattattt tgctgccttt ttctgtattg ccaaattcat caaaataagt
    13921 atgacaaatg ccggctccgg tttcaattac cggaattttt gagtgtgtcc gtacaaagtc
    13981 gattaatcct tttcctccac gtggaataac taaatctaca tatcccgttg cattgagcaa
    14041 atcagctgtg gcttcgtgcc cagatggcaa caaggtaatg atatccggat ttagaccaaa
    14101 acgatttaga acttcacgaa tcaggctgac aatggcagca ttggaatggt atgcatcact
    14161 gccacctttc agtacgcagg aatttcccga tttcagacag agtgaaaaaa catcaaaagt
    14221 tacattggga cgagcttcgt aaacaatacc aatcacgcca aacggaacgg taatttttga
    14281 aattttcatg ccatttgggc gaacagtttc catcaatgtt tttcccaaag gactaggaag
    14341 accggcaaca tttcgaatat ctgccgctat attttgaagc cgctgttcgt tgagcagaag
    14401 ccgatcgtac ataggatatg ccttttccat agcggcgagg tcttctgcat ttgcttttaa
    14461 aattttttcc gaaccgatag ctatctcatc ggcaagggca agtaaggttt cggaaattgc
    14521 tttatcgtcg accagttgaa gggtgcgggc agcatttagg gcagcctctt tccagtcgta
    14581 agaatgattg gtagcgttca tcatcaaaaa attttatagg ttgagagcta aaagttaact
    14641 gaatgactgc aaaatgaaca tgcaaagagt ttacattctg aagtcgaaac gttcactgcg
    14701 ggtattttca taaatttccc gatcgaaggt ataatacctg gccggtttgt gggaaactcc
    14761 gctttgcaat tcgtccgttt gaatcagata aggcattttg ttgaccttgc gtctgaaatt
    14821 gcgtttatcg tatgttgtgt caaaaattgc ttcataaact ttttgcattt gagtcagtgt
    14881 aaatttttcg ggcaaaagtt caaatattag tggttcgtgc agcaaatcgt aacgtaattt
    14941 tttcaaagct tcgttaaaaa tgtcattgtg atcaaacatc aattcccgaa tttcataagc
    15001 attttcccat tttgcttctt caagcagttt gctattgtcc acatcatgaa tatttaccag
    15061 cgaataaaat ccaaccgtta aaactctgtc atcctgcaag cgcggtttta cccattcgta
    15121 atctctttcg cgtcgcttga ggcggtcagg atcactaaaa accataaact gtttcatgaa
    15181 aatattttca agcccggttt gctctttcaa aatacgagaa gcggttgcat ctatttgctc
    15241 gtttcgacgg acgtggttgc cgggcaattt ccattcgatg agaatatttc cttcaaatgg
    15301 aacatattcc cttcgcacca gtactttcag taaattgcct tcgtaaccga aaataacaca
    15361 atcaaccgaa acgcttggca gagtttggaa ctctctattc tctaaaacgt tttcaaattc
    15421 gggcatgata aaaacaatat aatttttaac ccgacaaagt taagaaaaaa agatattttt
    15481 cgctaaaaaa ttttttctca aaaaattgaa aatcacggtt tcaaaaaaaa atttttgtac
    15541 ttagtgtaaa aaaaacacat ttttatttgg agaataaaat tttagcctct atatttgtcc
    15601 ttgaaaattt gttttagcta cataaaaatt taatcaatta tttttttaaa actattaata
    15661 tcatgaaaaa caaaaggtta aatctgtttt acctcctttt attcttcatt ccgatttttt
    15721 ctgctattag tgctcaggaa tccgtcagcg atcgtgttga agaattgctt tccaaaatga
    15781 ctattgagga aaaagtcggg cagatggctc agataacttt ggatgctatt ggcaacggag
    15841 ctaccgtttt tgaaagcaaa ataccttttg ccattgattc ggtgaggttg cataaagcca
    15901 tagtcgatta tcacgtcggc tccgtaatca ataccacaaa caattacgct ttgagtgcgc
    15961 ttcaatggaa tagtattatt gaccaaattc agaaaacagc aacaactaaa acaactctgc
    16021 gaataccggt aatttacgga atagatgcta ttcacggggt aacttacacc tccggagcta
    16081 ctcttttccc gcaacagatt gccatagccg ccacttttaa cccgggaaat gcctataata
    16141 tggcaaaagt tacagcttat gaaacacgag cttcaggcat tgcgtggaat ttttctcctg
    16201 tgctcgactt aggggctgat cctcgattct cacgacaata tgaaggattt ggagaagatc
    16261 cttatttggc ttcggttatg ggaaggcaag caataagagg ttatgaagga attaccgata
    16321 gcattgggca tgcagaaaaa gtagctgcct gtgccaaaca ttttttaggt tattcggttc
    16381 ctttttcggg gaaggatcgc acgcccgcct atattcccga tcatattctc agagaatatc
    16441 atttgccttc ctttaaagct gccatcgatg aaggagtaga atcggtcatg atcaattcag
    16501 gaattattaa tggagtttcg gttcatgcca attacaatat cattaccaaa ctgcttaaag
    16561 aagaacttgg atttcagggt gttgtggtaa gcgactggat ggacattatc aatctcgaaa
    16621 aaagggataa aattgcaaaa aacaataagg aagccattgc tttggctatc aacgcaggca
    16681 tagatatgtc gatggttcct tacgattacg aacagttttg caaggatttg acagcgcttg
    16741 tgaaggaggg aaaagttaaa gaaagcagga tagatgatgc cgttcgtcga attttaaagt
    16801 taaaattcaa actcggttta tttgaaaatc ctgttacaaa tttgagcaat tatcccgact
    16861 ttggttccga caagcatgaa aaattggctt acaaggctgc cgctgatgcc attacgttgc
    16921 tgaagaacaa aaacaatatt cttccgttga acaaaaatat caagatattg gtaaccggtc
    16981 ccaatgccaa cagcatgaga agtttaaatg gaggatggag ttattcgtgg caaggtgaaa
    17041 aaaccgaacg ttatgctgct caataccata ctattttgga agctgtacag caaaaaattg
    17101 gcgaaaagaa cgtcctttat gagccgggcg taagttatgt ggaaacgggt caatattttg
    17161 aagagaaaga aaacggtttg caaaaagcag ttgctgcggc tcgtaaggta gatgccattg
    17221 tactttgttt aggggaaaat tcttatgcgg aaaagccggg agatttaaac gatttgaatt
    17281 tgagcgaact gcaaatcaaa ttggccaaat cgctttcggc agtcaataaa cctattattt
    17341 tggttttgaa tgaagggcgt ccgcgttgct tcagtgctat tgaatccaaa atgaatgcca
    17401 ttgtacaaac ttatcttccg ggcaattacg gaggcgatgc attggccgat attctttttg
    17461 gagatgtcaa tccttcggga aagcttccat atacttatcc ggcttatcct aattcgatag
    17521 tgaactatta tcataaattt tcggaagaac aaaaaccttc cgaaggagct tataaatatg
    17581 aatcggatta taatccacag tatgagtttg gattcggact tagttacact acatttgcct
    17641 acagcaattt gaaaatttcc tcttcggttc tttatcccaa cgaattaatt gaagtcagcg
    17701 tggacgttac caatactgga accagagaag gaaaggaaac ggtacttttg tttaccagtg
    17761 atgtaatagc cagtataaca ccggatatga gaagattgcg acggtttgaa aaaatcaatt
    17821 tgaaacccaa tgaaacgaaa acggttaaat ttactctgtc ggccaatgac ttggcttttg
    17881 ttaacacgga taaccaatgg acaattgaac cgggagaatt tgaaattaaa ataggcaatc
    17941 tcaaaaaaac aataaattat caatcaacaa attaaccaac taatttatca atcaatggaa
    18001 aatgtaaaga aaaattccgg aaaaaaaatc gtgtctcggt atgttacgcg atttttaaaa
    18061 agatggttgc ccttactggt gattattgtt atgccgctgg cattgtcggc gcaaaaaaca
    18121 acgattacag gagttgttgt cgacagcaat aacgagcccg ttattggtgc aactgttttg
    18181 atcaagggga ctactacggg aaccgttacc aattacgaag ggaagttcac catcgaggca
    18241 tccagcaatg atgtgctggt agttagctat attggaatgg ttccgcagga aattaaagtg
    18301 ggaaattcaa aaaacatcaa tgtagtgctg aaagaagaca ttaaaattct ttctgaagtt
    18361 gtagtagtgg gatatggaac gcaaaagaaa aaagatttaa ctacggcagt atcgaccgtt
    18421 tctgctgaag attttaatgt tcgacccatc gtttcggcag gtcaggcaat tcagggaaaa
    18481 gctgcagggg ttcaggtaat tcaacctaat ggagcgccgg gaggagaaac ggtgataaga
    18541 gtacgtggaa cgacttcttt taatgggaat agtgacccat tatacgttgt agatggagtg
    18601 ccggttgata atattaattt cctttcaccc aatgatatcg agaatatcaa tatacttaaa
    18661 gatgcttctt cggcagctat ttatggctct cgtgcagcaa atggagttgt tattattact
    18721 acaaaacaag gcaagtcggg tgagtcgaaa attgcgttta atgcccaatt gacagccaaa
    18781 aatgttacta ataagataga atctcttaat gccgctgatt ataaggattt aatggaagag
    18841 gttcgcccgg gttcgtcttc tcaaatgagt tccgatgata taaccaattg gtatgatgaa
    18901 gtttaccgca ccgggttgca acaaagttat cagctttctt tctcaaatag tactgaaaaa
    18961 acgagttatt ttctttctgc cggctacctt gatgagaaag gtattttccc tgcatctttt
    19021 tttaagcgtt actctttcag agcaaatatg accaatgaga taagaaaatg gctgagtatt
    19081 ggttccaata tatcgtattc agataattcc aacaatggca taactaccgg tgctggttca
    19141 aatcgaggcg gagtagtgtt ggctttaatt aatcttccaa caagtttacc ggttatcaat
    19201 cctgaaacag gatattataa tcgtagtttt tatggtcaga acattaccaa tcccgtagaa
    19261 gcaatggaaa acgggaaaga cgatattacg cgtgaaaacc gattaatagc ttcaggtaat
    19321 gccatgataa cttttatgaa aggaatgaat ttcaagtcaa cttttacgct cgacagaagg
    19381 catgggttac aaacgggttt tagtcctgtg atacacgtta caggtattga cgaatatgga
    19441 agcgcatggg acaatagaaa tatgaatacg gttctgacat gggataatat ttttaattac
    19501 cagaaatcgt ttgataagaa taatattgaa ttgatgatgg gttcgtcttg gactgattca
    19561 aagtacacca atagttggat aaatggttct cattatagaa acaacaaaat aaaaacgctt
    19621 aatgttgcca acaaaatatc gtggaataat acgggaaccg ggggctctac atggggaatt
    19681 atgtcttact ttggacgtgt ttcgtacaat tacaacagta agtatttaat tactgccaat
    19741 ttcagggctg atggttcttc tcgtttacat cccgatcatc gatggggata ttttccttct
    19801 ttttcggctg catggcgtat atcgtccgaa gaatttatgc aggggctcaa ttggctcgac
    19861 gacctcaaaa tacgtggtgg ttggggacaa acgggtaatc aactcgggct cggcgattat
    19921 gcctatttgc agagatattc cgttacccgt caacaatggt ggataaccgg tcaggaaaat
    19981 attttaccaa tcattactgc cagcaatttg cggactacag acttgacttg ggaaactaca
    20041 actcagtcaa atgtgggtat tgattttacc gcatttaaga gcaggttgaa tattattatg
    20101 gattattatt acaaaaagac caccgatatg ttgctcaaca ttactttgcc ttcgggggcc
    20161 aatgtaacaa gtattcagcg taatggcgga ttaatgcgca atacaggttt tgaactttcc
    20221 gtaaactcaa tcaatatcac gaataaaaat ttcaagtgga cttcagattt ggaattttcg
    20281 ctcaatcgga ataaactggt aaagcttgat ttaacaaaga tttattatga cgctgctact
    20341 tccgatgctt ttcatgggat tcagattgta cgcaatcagg aaggacgttc tcttggcggt
    20401 ttctacggtt acattagcga tggggtagat tcagaaacgg gggaacttat ctatagagat
    20461 atcaatggag acaataaaat aactgcgtct gaccgaactt ttattggaga tcccaatcct
    20521 gattttattt atggatttac caatacattc acgtggaaag gtttcagtct tagtattttc
    20581 ttgcaaggaa cttatggaaa cgatattttc aatgcttcaa gaatagaaac ggaaggaatg
    20641 tatgatttaa agaaccaatc gacgcgtgtt ctggatcgat ggagacgtcc gggtatgata
    20701 accaccattc ccaaagccaa cttcaatctt caaccttcgt cctattttgt agaagatgga
    20761 agttatttaa gattgaaaga tttgacatta tcctataatg taacggctcc atttttgaaa
    20821 aaatcgggaa tctctcggtt acaaccttat ataactgctt ccaatttatt gacttttaca
    20881 aaatatttag gtatggatcc tgaagtcaat gagtggggta attcaaacgc tgttcaggga
    20941 attgactggg gaacatatcc tcaagcaaaa tcctttgttt ttggttttaa tattgaattc
    21001 taaaaaattt attactatga aaataataaa atatatactg atttccgctg tggtatttgt
    21061 tgcagcatca tgctcgctcg attataaccc cattgatacc tattcggata ttactgaagg
    21121 aattgatacc acatctgcag ccagtcaagt gattttaaaa gataaggctg ccgttctgaa
    21181 tcaacgacag gtactttatc agcgactgaa agacggtcag gaacattggt atcttgatat
    21241 gttgttgttg gcagaatctc attccgataa tgcttacgcc ggatccccaa atgcggaaac
    21301 aacacctttt gaaataaatt ctattgaagg atctaatcca aatcttaaac gtgattggaa
    21361 tggacacatg gataatattg cccaagccaa tcgacttatt caaggcattg acgagttgaa
    21421 agatccttcc ttgacggaag cagaagctaa acaaattcgt gccgaaggac ttatttttcg
    21481 ctcactggtt tacttcgata tggtcagaat ttggggtaga gtgcctctta taactactgt
    21541 aggaaaagac ataacggccg ataatatcga agaagtttat cctgcgtatt ttcctcagca
    21601 aagtgaagaa attgatgttt atagacagat agaagaagat ttgttggaag cagtacgata
    21661 tgcacccaac aatgatccga gcaataaaac cctacttacc aaatcggtgg caagagccct
    21721 tttggcaaaa gtgtatgccg agaaaccatt gcgtgattac aataaagtta tcgcttatgc
    21781 cgattctgtt gcagccgatg gttttgaatt ggtttccgac ttcagcgatt tgtggggtgt
    21841 gcaacttcaa aatccttctg cacctccggg cgaaaacaat tttgccattg atgcaaaaaa
    21901 aagaaacaca tcggaagcca ttcttgaaat ccagtatcct gttggatcag ggaattgggt
    21961 aacatggatg tttggccgtt cgcttgacaa ctggaatttc tggttcgatt gggctaaatg
    22021 gataactcct tctcgcgatt tgatcaatgc ttttcaggct gccggcgaca taaaacgtta
    22081 taacgaaagt gtggtttggt attcttgcag ttggaatatt tattatcctt ccaacaatta
    22141 tccatttatg tacaaaaccc gttcggccta taacagcatt attaaaattc gttatgccga
    22201 tattctgtta ttgaaagctg aagcgttaat tcaaaccgga gacttgagtg gtgccgcttc
    22261 aattataaat gataaaattc gaaaccgcgc agggcttgct cccttgcctg cttctgtaac
    22321 aaatgacaaa aatgcgttgt tgagtgctta tctgaatgaa cgtcgtcttg aattggcttt
    22381 cgaaggacaa cgctggtttg acttggtaag attggagaaa gtggaagaag ttttgaatac
    22441 gcttccttca agagattcag gtcgccctgc gctccgttat ccttacgata aatactcata
    22501 taaactccct attcctcagg atattataga tcagaatcca aatttggtac aaaatccggg
    22561 ttattaataa ttaaaattct attcaaatga aaaatataat caaactaaca atattcggac
    22621 tgctggggtt attcgcaaca tcttgccagc aagaagaact gagagtgcat tatcctgcct
    22681 ccaaacccgt ttttgacagc attagcgtac cggaaaccaa tattgtttat ggcgattcca
    22741 tcacgttata tgtaggcgtt tccgatcctc tgactccatt atctacgctc gaaataaaag
    22801 tggttgtaaa cgacgaactc attgcttccg aaacgatcag aacaaaaggg aatcaggcaa
    22861 cctatcaaca acgttacgga attccttttg tggcgcgtat gcccgataat gcagaagtgg
    22921 aaattcattt gacttccatt aatgtggaag gaaccaaaac ggatagcatt tacgtaaata
    22981 ccatagccaa acgccctact attacggatc tttatttggt tgttccactt aaatcgcctg
    23041 tcaaaatgaa attgaccgat gctcaaaatt atatttatac ggctgacaat ttaagtctgg
    23101 aaaatactgt ttctttccga ctggctacca aattgaaata tggaataagc atcgattgga
    23161 caggtttggt attcggcaaa gtaggaaatg gaattggatt gatcgatatt aatggaagtc
    23221 ccattactct gtctgatgca actttgatag gttacaagaa aattacggtt gatctgttca
    23281 actttactgt taaaggagaa ggcgacaagc tgcaaccggc tacagcaatg gatatcagta
    23341 ctttcggaac tgtttcgctt tcgtcgacag attacttgaa tgttactacc aaagaagact
    23401 ggaaaacgaa aaaaatgtat cttggaaaag gcaccgaaat gaccatcagc ggtattcaaa
    23461 cgacaaatct tgccaataat ctggctccgg atttctttga ggttacaggc tcgaatacgg
    23521 caaaattcct gggcgaaacc ggcatttata cggtttatta tttgcccaga ctcaattttg
    23581 tatttgttga acagcccgat gccgtatatc ccgatgccat gtggctttgt ggggtaggtt
    23641 ttggcccagc tcgaacgcca catataaaaa cttcttcttg gaattggaac acgccgctcg
    23701 aatatcagtt ctgcagaaaa gtttccgatg gcgtttatca ggcaacattc tatgcacttc
    23761 atcaaattga cgatactgca gcagaaccct ggagattgaa gttcagtgtg aaattcttcc
    23821 atcaaagaat gtggggcgga gaagaagatg cccgcactta cacattaaat gtcaatgggt
    23881 tgctgtttgc tccaactgaa agcgatcaag gtaattttgc cggaaccgat gcgtttaagg
    23941 ataaacccgg gatttatcga cttaccattg atgtgaacaa taagacggct aactttcaaa
    24001 aaataagtga ttaattattt acatccatcc cgcaaacaac ttttttgcgg gatggataat
    24061 tttatttgag atgaacaaaa aaagcatatt cgtattttta tttcttcttt gggtttttat
    24121 tttttctacg ggatgtaagc attcgggaga tgaaccggtt cctactccta cacctcagga
    24181 ttcggtaggg gatgtaagtg tatggttgac caccggcaat gaatcttgtc tgttaaaaaa
    24241 aatgacaccg attaattttt ccagagactt tgccgatttt accattgtca tcgatactac
    24301 aacaacttat caatatattg atggttttgg agcagctttg accgggtctt cagcacattt
    24361 aatacatcaa atgagtcccc aaaacaggaa aaaacttttg gacgatctgt tcgaccctga
    24421 aaaaggaatc ggtatcaatt atttgcgaat aacaattggt tcttccgatt tttctatagg
    24481 gacatacagt tattgcgaca gtccagatat taataaattc gatattcccg aaatagaccg
    24541 aaatgaactt ttgccggtat tgaaagaaat tcttgaaatt aatccatcga tgaaaattct
    24601 ggccagtccg tggagtgcgc cggcatggat gaaaagtaat aacagcatgt atggcggaag
    24661 tttgaaaggt gaagaagttt acaatgactt tgcagaatat tttgttcgtt atgttcaggt
    24721 ttttgaacag gaaggcattc atattgatgc gctgaccatt caaaacgagc ctatgtacga
    24781 aacaggagga tatccgacca tgaaaatgct ttggcaggaa caaaacaaaa ttattcgcga
    24841 ttatttgggt cctaagttta ttgccaacgg cattcaaacg aaaattgtga tttgggatca
    24901 taatttcgat atgtcctatt accctatcaa tattctaaat gatccggtta cccgacaata
    24961 tgtaaatggt accggctggc atggatacgg aggaaatgct tcggccatcg atcaggtaac
    25021 tgcactttac cccgataaag aaacttattt taccgaaatc tcgggtggag gatggaatac
    25081 ggatacccgc atgggaaaca tgttttatta catgaaagat ttccttatgg ctacggtcaa
    25141 tcgtggctct aaaaattttc ttatgtggaa tctcgcttta aatcctcaac atggaccaac
    25201 taccacaact tggggtggct gtcaggattg cagaggtgtg gtaactatca acgaaaatga
    25261 gacttacatt cgcaacgagg agtattattt gctgggacat tttgctaaag tggtacgttt
    25321 gggagctgta cgtgtaaata attcctgtta caatttacct tccggcatga ccatatcttc
    25381 ctttatgaat gccgatggca gcaaagtggt ggtcattttg aacagaagcg ggtcatctcg
    25441 gaaatataat gttcgctgtg gaacccgaaa attttactat cagcaagtag atgaatcggt
    25501 tgtatcattc aaattctgat tttttatcga gaaattattt ttttttgtta aataaaaaag
    25561 tttatccatg aaaataagag ctttattttt tttaatggcc tttgccattg ttaccaattg
    25621 catttcggca gggaaagtaa ctgtttggtc atcgctgccc gataccaaag ctttattggt
    25681 aaaaaagagc atgaagttga attctaaacc tgtagaaatt aaaaacaagg ttgaaatttt
    25741 tcccgatcaa acctgtcaat cggttgaagg tgttggagct gccctcaccc attcttcagc
    25801 ttatgttttc catcacaatc tgacagccca aagaagggat tcattgttta aggtgctttt
    25861 tactcctgca ggtatcggca tcaattatac gcggctttgc attggtgctt ccgactttgc
    25921 ctttaatctt ttcagttaca gcgaaacgga agatttttcg ttaagtaatt ttaatattca
    25981 ggaagatgaa aaagatgtaa ttcccatgct gaaggaaatt ttggccatca atccaaactt
    26041 gcaaattttg gcatcaccct ggagccctcc cggatggatg aaaacttccg gaagtatggt
    26101 tggcggcaaa ttgcgtcccg attgctacga tgtgtatgcc gaatatttga tcaaatacat
    26161 tgaggcttat cgacaacatg ggataaccat tcatgcgctt acggtgcaga acgaaccgga
    26221 atacggaact gcagcttatc cttgtatgga tatgacggca aaggaacaac agatttttat
    26281 ccgttatttt ctgggcccca aaatgcagaa aaaaggaatt cagaccaaaa tcattctttt
    26341 cgatcacaat tgcgacaacc cggactatcc gataagtatt ttaaatgatc ccgatacaaa
    26401 gaaatttgta gatggttcag gatttcatct ttataaagga gaaatatctg cgctttgtaa
    26461 agttcatgaa gcacatcccg ataaaaatat ttatttcacc gaacaatcgg gtggaggttg
    26521 ggctcccgac tttgatcaaa acgttcgttg gtatgttgga gaattgtttg ccggagccat
    26581 gaactgctgg agtaaaaacg ttctcctctg gaatttggct ttggacgaaa acgacggacc
    26641 aaaaaatttc ggttgccaaa attgttgggg cgtagtacgc gttacttcgg atggaagaat
    26701 cgaaaaaaat gctgaatttt atgccattgg tcattatggg aaagtggtac aaccaggagc
    26761 tgtccgactc gtttcaaatc aggatatttt aaaaggcgtg gcttttcgca atcctgataa
    26821 cagttttgcc tatctggccg tttattatgg cacaactccg caaaccatag gtattcagtg
    26881 tggaaacagt tcttttgttt atacttttaa acccggagaa gtagtatctt ttctgtggta
    26941 gatgtaaaaa attgctatcg gttgcagttc agataaaaat ctgtccaagc cgatagcaat
    27001 tttttttgtg aattttcgag cgaaactttc gctgaatatg tttctaaaat gtaatttttc
    27061 tcaagaaagc aatttttttt ttaccatttc cgaaatcgct ttcccatctg cttttccctc
    27121 caacttgcga gtggcaattc tcactacttt tcataaattt tgagcccctg tgcactggtt
    27181 tgggctatga tttatctgaa agttactctc tgaaaatctc agcttacttt tttacacctg
    27241 taagtccaat tttgaaaaaa cgcaatcaaa gaaattttac ttccttaaaa taatatttgg
    27301 ctgaggttcc tttttcggtt tcgagttcca aagcgccatc cggataaatg cggctgattt
    27361 tggcccgaaa tatttctgtc cccgcctgat aaaaatggta gccatttttg cgatacaagg
    27421 attgggcata aacacttctg attttttcgg attcccactg cccgtacacc tccaaaattg
    27481 acgcaacaat tcgatgcagc aactttttcc gattaaaact tttgccggca atttgtttca
    27541 gagaaatcgg atggggtgcg tcgctgaaaa attcggtctg attgacattc aagcctacac
    27601 ctattacagc ttttgatatt ttgccccgaa aaagggaagt ttcgatcagt attccggcca
    27661 gcttgctatc tttccaatac atatcgttgg gccatttgat ggtaatatcg tccgaaactg
    27721 tgtccaagac attttttatt cccacactca ccgcttgcga aatcagaaac tgctcttcaa
    27781 taggaatatg atgcgggtac agtactatgc tgaaaagcag attctttccg cgagccgatt
    27841 cccaagaatt tcctgcctgt cctttccctg ctgtctgata atctgcaacc acaaccaatc
    27901 cttccggaat atctctttct ttaaacattt tccagcacaa cacattggtc gatatcgttt
    27961 gaggaatata aaaaacattt tccgtgagca tgcaaaacaa tttatttgaa aaccgacagc
    28021 aacagttctt cgattttttt ggttaccgct cttgaagttc cctgcgcatt gtttaccatg
    28081 acgacaaaag tataggtttt tccttcgttt tttacataac cggcataaca tttcactttt
    28141 tcaatgcttc cacttttggc aaaaactttc ccttccaatg gcgtaccggc caaaaacgaa
    28201 acaagagttc ctgttttccc agcaatgggt aaagaattga aaaaatcttt actgaaagca
    28261 cttttggaat acatgtaatc cagcaactcg gtaataaact gagctgaaac agcattcatg
    28321 ggcgaaagtc ctgaaccgtc gcacataaag agctgatcta cgggaagtcc tttcaatttc
    28381 cacaaattac gtatggtttt aactccgcct ctgccggtaa cgggaacacc ttcaccaagt
    28441 cccaaataac gaaaaagaca ttctgcataa tgattattgc ttctttcgtt ggtttgcttg
    28501 ataatatctc tcagtggagg agaaaaatgc gtataaacaa cttgcccatc aaaaggcgtt
    28561 gaaaattcgg caacaggagg attattaact gtaatgccac tctggatcaa cttatcggaa
    28621 aactgctttg ccaaaagcaa aaccggattg ggaatatcac cttttactac aaattctgct
    28681 ctgttggctg gaatttcgcc ataaagccga cgcaaattgg aattgggcat accgcaaaaa
    28741 taggcactgt cgaacgtaat ctttgaactt tttaaataat tttctatcac caagtccggc
    28801 aaatcgggaa gagtgcggat aatggcaggc gtggtcccaa tcggtccgga acgtaaaacc
    28861 aaacgaaaag tattgtcgag ataagaaatt ccgaaaatgg aaggagcata ataatttcca
    28921 atatcttccc atatccagaa aggactgact ccttcttggt tgtaaatact ggcatcggct
    28981 ataatttttc cttcaatttt actgattcct gcttgtttta tagccaacac ccattggtca
    29041 agaaaatttt tttctcccaa aaattccgaa cccagtgtgg ggtcacctgc acctttaata
    29101 attagattcc cctgtaaaac atgttctttg gttacattgc cggaaatctg cagtttcgtt
    29161 tgaaatctga aattcgggcc cagcaattcg agagccgaag ctgtagtaac cagttttaaa
    29221 gtagaagccg gaataatgct tttgttggca ttgtaacgtc ccaccacttt cccttttccc
    29281 gtttcgcgta tcagaaggct tacattggca ttcttcaaca aaggatgatg aataaaatta
    29341 tcgacaggag tttgagcgac aaggttgagc acaaaaaagc taacaaaaac gttgaaaaaa
    29401 attttcttca taaaataaaa atttcagatc atgttgattt atagataaat gtatatctca
    29461 ctgtaatgct gtaaaaaata aatggtaata gaagtacatt gtttgccgtg acctgttcta
    29521 tagcaatagt taatctgcac aaaaacaacc aaattatgtt cccaaaccat ttcaatattt
    29581 atttctcaat aaatttttat tttgtacaaa ttcgaccctt atcttgggta ttgagtcaaa
    29641 agtagaagtt tcaaatgtac aatatttttt taaatttgtc attccttccc gtgaattaaa
    29701 aatatagcaa ttatcgtttc acagcaaaga gaatgatttt aatatttttt cattgattaa
    29761 agttttgctt attttcaact taaaaattgc gtcaagttag tgttataatt aaatttaatc
    29821 ttgtaacttt gcgaaggttc gttatcttta ctgaaaaaag tatgaaaaat tttactttcc
    29881 tgttgttgtt ttccgttttt ggcggggaac ttttatgcca tgcttcagga gaacaagcaa
    29941 atataatttc actaaaaaat gattctttgc ttttttcatc agatcagatc gagcttgatg
    30001 ctttcgattc tattcctgaa aaaaacataa aggaagtagt tgtatatggc atttcttctg
    30061 gtagattgac ttttccactt tcgttggtaa gtaaaacatc acttctgaat agttctttta
    30121 ccacccctgc cgatgcgttg caaagagaaa ccggcatttt catgtcccgc gatggaattt
    30181 gggctacttc agttaatatc agaggtttgt ccgaacaaag acttcttttt ctggtagacg
    30241 aagatcgatt gttaaccgca accgatattg cggcagcatt gtccacagtg gatatgaatg
    30301 ctttggaaag aatagaagtg atcaaaggag ccggctctgt tttatacggc acgggagcta
    30361 tgggcggagt ggtgaatttt gtgattgagc gtccagcatt tactcctttt ttggaaagtc
    30421 agggaaatat tcattcgtcc tttaattctt ccaatcaact ttggagcaac cacgcaaaat
    30481 tgcagttaac caatcgccag tggtatttgt cgttgaacgg aagttatcgt actgcggata
    30541 atatgcgtac acctgaagga aaaattccaa acagtcagtt taacgatgct tcctggggaa
    30601 tgcaagccgg catgtcgtcg gtcgccaatc aggaattatt ggttaattat cagcattttg
    30661 aagcatggaa tgtcggattg cctggcggta gcgcttttcc accaacggca acagttcgat
    30721 acaaaaacgt acaaagaaat ttatttgatg cagaatatgt tttttacgaa ataaccgatt
    30781 atttaaaaca acttcgcctt aaagcttata cgcaaaatat cagcagagac gtagaaaata
    30841 ttgtcaatac atctacagcg atttttcctt ccagcaataa taatacttca ggcatcaaag
    30901 ctgtgggcga ctgggaattt actgcatacc acaaactgac attaggaact gaaggatggt
    30961 tgagggacgc caaaacatca cgcatcaaaa aagttaattc aggaacacaa ataaacgttt
    31021 ttggagaaca acccacaccc aatgcacaag ttcaagatat gggaattttt gcacaataca
    31081 gttgggaatt ttcgccacgt tactggatgt tggatgccgg aataaggttg gattacatga
    31141 aaaccaccaa cgacagcgct ttccagccgg tgtacaaata ttcttatttt cctgaaaatc
    31201 catccatgcg aatttatgaa gatattccca gaagtttgct tttcaaagcc gatgaaaaaa
    31261 atgaattttc ctattctgcc catatcgatt tgatttatca tcccacacgt cgtcaaatgt
    31321 tggcactttc tgtttccaat tcctataggg cgccttcttt ggaagaatta ttcaaatata
    31381 tcgatcaagc cggaaccttg cgtgttggga atcctgacct aaagcccgaa aaaggctttt
    31441 tttcaaactt cagctatact ttgtcggaag atcattttta tataaaaacc gatctttttg
    31501 ccaactacct ctttgatttg attgccgagc agcatggaac ttacacttat caggacgaga
    31561 agggaaaagt tacgcaaaga gaggcactga tcaatacaaa tatcgacaag gcactctttt
    31621 taggaggaga aatggaactg caatggaaaa taaatcctgt gttttccgtg ttggctaatt
    31681 tgtcctacac acagggaaga aatattgaca aggatacttt tcttccacaa ataccaccta
    31741 tgcatggatt gttttcggtg aactaccatc aaaaagaact ttttgatgcc tctgtttcag
    31801 ctttgtgggc agcaaaacaa ggaaaagttg ctccgggtga aaatccgacg aatggccatt
    31861 taatttttaa tgccgattta catacagaaa aattcagaat caacagaaat tatgttcgtt
    31921 tttttgccgg tgcagagaat atttttgata aagcctacta caatcatctt tccacaacac
    31981 gtggagtatt acgtctggaa ccgggaagaa atatctatgt aaaggccgaa ttgggttggt
    32041 aaaagagttt tttctgaacg aattatttca ttatttcaca gaaaaataga taaaagagga
    32101 gtaaaaaaat catcgaaagc tgacttttta ctcctcttgt ttgaatttta attttggtct
    32161 ttattatttt cgaaaatcag agaaaagatt tttctttgct tttcatcagt tcaaaaaaat
    32221 tctctattaa aacttcactt ccggagcttt ttcgcttcct tcctgtgctt caaaatatgg
    32281 ctttttctcg aaaccgaaca ttgctgctat gagggaagtt ggaaaacgtc gaatggtagc
    32341 attatagttt tttgctattt cgttaaaacg attacgttcc actgcaattc gattttctgt
    32401 cccttccagt tgtgcttgca attctatgaa attctgattg gctttcaaat caggataatt
    32461 ttcggtaatc atcagcaatc ttcccagtgc ggtgctcaat tctccctgag cagcctgaaa
    32521 ttcctgcaat ttttccggcg taagcgaggc tggatccaca gtaacttgag tggctttggc
    32581 acgagctgcc gttacggctt ccaaagtttg actttcgtgt gcagcatacc ctttgacggt
    32641 tgctaccaga tttggaatta aatcggcacg tcgttgatat tgattttcaa catttcccca
    32701 ttggctcgaa acagcttctt ccgccgaaac caatttgttg taacgggaaa ctccccataa
    32761 aaaaaacagt gccagagcgg cgataacaat gataatggtg ttttttttca taattttttt
    32821 atttagaaaa tgtgtaattc attgaaaaaa gtgttttagt aagattgatt aaaatagaaa
    32881 ttgttatagt ttttgaaatt gaagaattta aaaatgtctt attttaccaa ccgctggttg
    32941 cgcctccgcc tcccgacatg ccgccaccaa aacttccacc accaaaacct ccgcttccac
    33001 tgaaaatact gccggctgct gctcctccga taaaggtact gttggctcgg gtaagtcgcg
    33061 gaatacgccg gtttttgttt tcttcgtagc cgcaaaattc acaacgatag gtaagccttt
    33121 ctgttccggc attgaaatag gtaggggcca tagtaagttt tttgtctttc agaataaaag
    33181 ctttggttcc acattttgga cattccgagt aagcgctggg cctatcgata gtaaaaatgg
    33241 cttcattttt acaattgtcg caaacaaata catcgtaatc gacagcatga agttgttctt
    33301 caaattgttg ggaaagtttc aggtaaacat cttcttcttt ttccggaaca aagtgcattt
    33361 gtcccccaca aacactgcag ggaaaaggtt cttttctgat tttttgaagc ctgtaattgg
    33421 catatatcag tggaggaatt gtcatgaaag gaagaggaag gagcaaaagc agataaatag
    33481 cttgcgataa gacaagaata gcgataaatg cggcaatagg taaaaatatt gccataacgc
    33541 tgacaatgcc gtttctttca ttttttgcag caatgtagcg tgccatatta ctgttgagtc
    33601 gcggatttat cttcacttta ttcatagcgt tacccatcca caaccaagtt aataccaacc
    33661 atataagata aaaacccaat gcaagcggca aaatttcttt ccaatttaca ggagctgttt
    33721 tttcaacttg aacgggttct ttcaaaatag tggcatagat acgttgaacg ccggccagta
    33781 tgcctgcatc ataatttccc tttttaaatt cgggaatcat gctttcaatt tgaatacgtt
    33841 tacagatagc atcaggaaga atcccttcca gtccataacc gacttcaaaa gtaactttcc
    33901 gttgatccat tacgaaaagt acgagtaaac cgttgtcttc cttttctttt cctattttcc
    33961 aagaattgaa gagttgctcg gcaaatgatt taatatcttc attgccaata gaattgacta
    34021 aaacgattgc aacttcggca cctgtctttt tttctaatga atcgagcaaa ttgttgattt
    34081 gactttcggt ttcggggttc aaaataccat caggattact gacaaaggca aaacgattca
    34141 gtttttttgg attgggaatc gtttcaacgg tgtaggtttg actccaaatg gaacatattg
    34201 caaagcttgt cagcaaaaga atgaaaattt ttttcatttc ctgatttctt gaataaattt
    34261 gtgtttcaat aataggaaga aacgagggtt acctcataaa tcaatgcaga atatggggga
    34321 atttttgaac ccgacccctc ttttccataa ccaagtgagc tgggaatgta aaaaatgaaa
    34381 gttgaaccat ccctcatttt ggtaattcct tcttgccaac cactgatgga actcgaaaga
    34441 tagtagtcgt acgttccttt ttcgatcaat gttccatcaa ttaaatatcc tttgtagttt
    34501 gccgtgatcc aactacttgc atttggcttt cttcccacgc tctgatgaat gatttgatag
    34561 caaagtccac tggatgtaac cacaaatccc gaatcggatt gatgtttcaa catccactgg
    34621 tcgttcacga ttttccaatc ttcataatta ttttctttac atcctgcgaa agaaaaaatc
    34681 agtagggtcg aaataaaaag aaagaaactc tttggtttca taagtcgatt atttttacgt
    34741 tttttggtaa gaaaaattgg attcaacatt tttttttaac aaaatgtttt atatttttcc
    34801 tccttgcata tatttgataa tgaataaatt aaattatttg ttgttttttc tgtagagctg
    34861 tgaaaacgat ttttttgcaa atttcggaac agttctttcg ggtccccatc cccaaaaatt
    34921 taagggataa aaagctgaat ttttcattcc cgcagggaaa aagtcaaatc cttttcgttt
    34981 cgaggcaatc agacgaaatc ctttcattgc tgcggtctcc agcgccgatg aaagatgttg
    35041 gtctaccgct tttctcctat ttgccaacaa aatgtcggga agcggaattt ttaccgggca
    35101 aacttctgca cattttccac aaagtgttga agcatagctc aggtggtaaa aatcgcgaaa
    35161 tccctttaaa aacggagaaa tggccgagcc aattggccca ctgtaagtgg tgttgtacgt
    35221 gtaaccgcca atggttctgt acaccggaca gccattcaga caagcgccgc agcgaataca
    35281 tgccaatgat tgataagcct catcgtctgc aaaaatacca attctcccat tatcgagcaa
    35341 aattacatac attttttcag ggccatctgt ttctcccgtt tgacgtgggc ctgtaaaaat
    35401 cgtattgtag gccgtcattt gttgacccgt accatgaacg gcaagcaacg gataaaaaag
    35461 tcccaattgt tccatcttcg gaattatttt ttcgataccg gcaaaaacaa tgtgaatttt
    35521 cgggaaagaa gtactcatta aggcattacc ttcattttcg gtaaccgaaa ttccacccat
    35581 atcggcaatg agaaaattgg cgccggtaac tcctatctcg gcttgcgtga atttttttcg
    35641 gagcagacga cgaacgaaat ccgataattg ttctggagta ctgtcggatg gggtatcgaa
    35701 tttctcgtga aacaaatcgg caatttcact ttttgatttg tgcatggcag gtgtaacgat
    35761 atgataaggc ttttctcctg ccgtttgaac aataaattca cccaaatccg tttccacaga
    35821 ttcacagccc aattttgctg cagcagcatt cagttcgatt tcttccgtag tcatcgattt
    35881 gcttttcacc agcaggcggg catcgttttc aaccagaatt ttttccacat aaacgattgc
    35941 ttcttcgctg ttgcgtgccc aaagaacttc ggcgccatga gaagaaatgt ttttttcaaa
    36001 ctgcaacagg tattcgtcga gatgcgaaag aacttcatgt tttatcgcag cagcctggtt
    36061 tttggcggct tctacatctt tgtagcgttt catgcctgtt tccacagcca agtcataacg
    36121 cgacatattg aagtcgattt tttttcgatg aacttcatca aaagcaattt tagaggcatc
    36181 gaaatggaat tttctggctg gtttcatgac aaaagtttta gaaccgctta ctttttgatc
    36241 tcaaaagtga aacttccaat tctgaaacca tccacaaaaa aatcggcacg atatgttcct
    36301 acttgaagaa tttcatctac tttccaataa attacgtcgc tcaatgcttc accggcatat
    36361 tcaaaatctt tttttgccga ataggcaata tatttgtttt cgtagagaaa aacgtcatct
    36421 ggatcttttg tcattacttc accatcgggc ctggtcagac gcaaataaag cgttttcatt
    36481 cccggcggac aggtaatgtt cttttgaatg gtataattaa actgtaaagt ggttatttgt
    36541 gaatatctgc ctgttttccg atttttgctg ttcaaaggag tcatgctgaa atttgatatt
    36601 tccagcattg aagcacgagt taccacttct gtcagatttt ctttttcttt ggataactgt
    36661 ttcactgttt cgctagcagc ttcgtattta cgtttcactt ccacgttttc tacgcgcaat
    36721 actttgttgg ctgcactcaa cgaatcgatt tgatgaacgt actgggccat cactgtccgc
    36781 aaagtggcca attcatttct taattccgca attctccggg cgttggttgc ttttgttatt
    36841 ctaagctcct gtaaaagctg ctgcactttc atcttttcat tctgaagcag ttgcaccaac
    36901 gaatcattcc gaatggttga cgaataagtg tcgtattcca atgcaaggtc tgaataatct
    36961 ttttccagct gttccttttc aaaattcatc atttccacta cttcagacat ttcagcatct
    37021 ttttgtttgt ttacttgtac gaaaaaaatt actgtaccga ttaacagcaa aacgacaacc
    37081 gacagtacaa taagaatcaa attattcttc ttcattcaga tagacataat taagttgtta
    37141 aatgatagaa attcattaaa tgaaataaac ttataaaaat ggcaaaactt tttccagatg
    37201 tattcctctg gaacccttaa taagaatatt ttgtccttgc aacgggcttg attccaaata
    37261 tttgattaaa tcttccacgt tttgaaaagt ccgatagttg tcgttgacct tttgaaactg
    37321 agatccaacg agcaaaacgt ttttaaaacc attttgttga agaagtagaa taatattccg
    37381 atgttcttct tcgctttgtt taccgagttc gagcatgtcg ccaataatca gcgtcttggg
    37441 ttctccttcc atccgagcaa aattctttat ggcagcgctc atgctcgaag gatttgcgtt
    37501 gtaagcatcc acaatcaatt tgtttttttg cgtgatggtc agttgcgacc gattattctg
    37561 tggaatataa ttttcaatgc ctcgttgtat ggcattgttt tctacaccaa agaatgaacc
    37621 tacagctatg gcagccaaaa tattttcagc gttgtatgcg ccaatcaatc gtgtttcaac
    37681 aataaaagtg ccttcgggtg tatgacaatt cattttaaga aaaacggaat tttccaaaat
    37741 ttctcccgaa attgcagccg gctctttatt gggaatgttc aaaccatatt caataattct
    37801 ctcactcgtc gaaaaacctg cactgaaagc catttcacgg aggaaattat tatttttgtc
    37861 gataaaaatt tttttgccat gccgaaaaac atagtcatac aattcgcctt ttgtgcgcat
    37921 gacgccttca aaggacccaa aaccttccaa gtgtgctttt ccgacattcg ttatcagccc
    37981 ataattcgga gccgcgattt ggcataattg atcaatctca ccgggatgat tggctcccat
    38041 ttcaataact accaactgat gttcgggctt caattgcaat aaagtcaatg gcacaccaat
    38101 atgattgttc agatttccct gcgtagaaag gatattgaac ttttctctca acactgcagt
    38161 aataagctct tttgtagtgg ttttcccgtt tgtgccggtt attcccagaa cgggaatatc
    38221 catttgttga cggtgaaagt gtgccagttg ctgaagtgtt ttcagtacat catcaaccaa
    38281 tataaaacga ttgtcaacag catattcttt ttcgtcaacc acagcgaaag cacaaccttt
    38341 ttccaacgct gaatgagcaa aagcattggc atcgaaattt tcacctttca atgcgaaaaa
    38401 aagagaattt tccggacagt ttctgctatc ggtagtaata atagggtgtt gtagaaatat
    38461 atcgtataat tgcatgaagt aaaataaaaa tttttacgct aattcatcac aaaaatagtg
    38521 ataatcctct gaatgaatgg aaacattcaa atcattttaa caaaaaaggc caaactcatt
    38581 catagagtta agccttttta tagaatgtag aaaatttatt tttctcgcag tttagagatc
    38641 aaatcctatt gaagccttga attcgtcgcg agtcaaagtg tagttttcac tgttgttggt
    38701 atccagcaat ccccagtcgt agcccactcg aagttgaacg ccattaactt ttataccggc
    38761 tcctacaccc atttgaatat caaaacgcga ttgccggctg ttgccttcgt cgtcagtttt
    38821 gtaataatca attgttgttt ctatcgtacc taaactactg gtaacctttc ctgtcgattt
    38881 cttgctgata ccgacattaa aacttggtcc accaaaaata aataccccaa cattattgcc
    38941 tgcaggaacg gtaaacgcta atcgaaccgg aatatctatc gaatgaaagg tggtggagat
    39001 tttggtatcg accaatgaat tttcgtaggt tttgctgctg gttaaaaaat tgtagaacaa
    39061 tccatactgc aaactcagtg gcccttgaat ctgcaattcg gctgttggcc caatatgaaa
    39121 tccatttaat gtgttgagcg tatcgccttc tcctacaaaa gtagaagaat ttccataacc
    39181 ggcttccaat ccgaaacttt gcgcattgag cgtaacagca gcaaacaaaa ttgctgtgaa
    39241 aacaaaaaac ttaactcttt tcatactttt tgattttaag ttgataactg aattttagtt
    39301 aagaaccaaa aacaaaaaat ataccaacgt gtaataaaga agtttttaca agtaaaaaag
    39361 ctgattttaa atttaaccta atttatttgc aaacagtaga ttaactaaaa ttcgtaacct
    39421 aaggaaacaa accacccact ttggtgaaga cttgcagttt tggctttgta caaatcgagc
    39481 aatccaaaat cgtaaccact ctttaaacga tattttctcc actgaatacc gcctccggct
    39541 cccagttgaa tattcaacct tctcaatacg ttatcgttat acaattcgga acgggtagta
    39601 tcgttaggaa tggtaacgtg ttgaaattcc tgaacaagtt cggtaagttc gggtgttaat
    39661 gtagaaacgg ttttttgggg ctgatataat ccgatattaa aatttggtcc ggcataggca
    39721 aacaatttta ccttttttat ggtcaaggtg tagttaagtc tgaccggaat atccaatgaa
    39781 tgtgagtatg tggtatagcg aacatacgta tcgtgtggaa aaagctgttc cttgaaggaa
    39841 tatagaaatg aataatagcc acctacagaa taactgaaat tgtttttcag ggcaaagtcg
    39901 gatgatacac ctacttgtgc accatcaaaa taagtggagc ttaaccctga accactgcgc
    39961 acctgattca aatagcctgc ttcgattctg tatgtctggg cggaaagagg aagataaaaa
    40021 tacaagatcc cggtcaaaat aaatacgggt tttaaagact ttttcatgta tatgcaattg
    40081 taaaaagttt tctgaggcaa agataagtct ttttatttta aagagaaaca agccctaaag
    40141 tgaaaactaa tgaagttatg ctttatcttg ttaacgccat tttctgtatt acttacgggg
    40201 atgattgatt tccacctttt tggttttctc cataatttta tttctttgtt cgacagcagc
    40261 tactacattt tcagccagtt gtcgaaaaga aaccgataaa atgctgtctt cttctgttgc
    40321 aatcggtttc ccggcatcgc ccgattcccg aatgctttgt actatcggaa tttgtccgag
    40381 caacgggatg ttgaattctt cagccagtcg ttttcctcct tcttttccga aaatatagta
    40441 tttgttttcc ggaagttctg caggcgtgaa ccatgccata ttttctacca agccaagtac
    40501 cggaacgttc actttttcgt tggtaaacat attgatgcct tttctggcat cggccaaagc
    40561 tacctcttgt ggcgttgtta ccacaattgc tcccgtaatg cccattgttt gtaccagtgt
    40621 gagatgaata tcgccggttc cgggcggcaa atccatgata aaataatcca gctcgcccca
    40681 atgtgcgtcg gtgataagct gtttgagggc attacttgcc attgaaccac gccataccaa
    40741 agcattgtcg ggatcgacaa aaaagccgat ggacagaagt ttaatgccgt atttttcgat
    40801 aggaacaatc agttctttcc cgtcaacttc ttccaaaaaa ggccgtgcat cttccaatcc
    40861 aaacattttg ggaacggaag gcccgtgaat atcggcatcg agaagtccaa ccctgtatcc
    40921 gagcgaagca agcgctacgg caagatttgc agcaatggta gattttccca cacctccctt
    40981 gcctgaaaaa acggcaataa tattacttac tcccggaaga atggagtttt ctttggctgt
    41041 tggaatttct tttttgaaaa ctgctgttat gttccctttt atgtccactt cttttcccac
    41101 ataagtcaac acagcttgtt cagctgcttt aacaagcgat tttacaaacg gatcgttggg
    41161 cttttcaaaa atcagagaaa agcttacttt gtttccttca atgcggatat tatcctgcac
    41221 catacccaaa gatacaatat cctgaccttt gcccggataa cgtacgtgtg aaagggtttc
    41281 cagaattaat ttaggatata gattcattat tttaatgttc taaaattttt aatttttagt
    41341 tgtttcatgt tttggccttc ttccaaaact tttatattga tcgtgctcga tttctacttc
    41401 gggttcgaga atttcgattc ttttttcgag tttgaattcg atatatttta tggggattcc
    41461 tctttcgatc cactgttgtt cataaaaggt tttgatgctg agtataggat gatcactggc
    41521 aatttcatat aaattgtcgg ttgaaaattc tatagggaaa tggttcaatt tcaacagttc
    41581 catcgtatag gtaaacaaaa aattgctgtc agttttcaaa tgaatcaaag cattttcgac
    41641 agtgaaacgg ctatacagtt tcagaaatcg ggatgaagta agtcttttgg tgattttttt
    41701 catttgtgga tcggggaaag tcaaccatat ttcactgact tcattctctc caaaaaagta
    41761 atggagcatt tcaatgcgtg tacgcaaaaa agctacattt gggattttct tttcaaacga
    41821 atatttagct cccgtccaca tacgtgctcc cttaatatcg acgccaataa agtttttttc
    41881 aggaaaaatt tcagccaagc cgacggtata ttctccttta ccgcatccca attcgacaac
    41941 aatgggattt gaattgccaa aaaatttctc gttccatttc ccctgcattt cgaaatttga
    42001 atgctcgttc ctcagcatct tggcaggaaa ctgaaaaaca tgtggaaaag tttccatttc
    42061 ggcaaatttt gccagtttgt ttttactcac ggtaccgaaa tttcttgtta cttgactttt
    42121 tcgattttaa tacccacatc gttaatgccg gcaaggatgg aatctcgcat cccttgaata
    42181 atttggtatc gataaattcc cgcacgagga aaactaactt tttgacgata aagaatcgac
    42241 atttcataaa cggaaccaac tcccgacccg agccattttc cacgctgatc tgcaagatag
    42301 cattcaatgg tgtctttttg caaggtgtcc atcaaaggag tagcttgacg aaggaaaaac
    42361 caaagattct gatacggata ttcgcctcga tgacggatat tgacgtaaac attataaaga
    42421 gaggttgtgt cagtaatggg gacatcgaaa gtcagcaccg aatctttgtt ccaacctttt
    42481 gtgggcagtg aacgatactg caaataaaca tcatttcggt tgcaggaaac catcaaacag
    42541 gtgatggaca aaagcaaaac aaatttactt gttgttgtta ttttctttgt tttgttcatt
    42601 ttttttgacg ggttttgcaa ttttattttc ggaggcagtt tgtccgtttg agtcggaatt
    42661 atttttctgt tttttgtttt tatttttgaa gcgtggattt ttccgttttc gctcgtcgaa
    42721 acgggtaaca ctttcctgac ctacaacatt ttcaaaatcc tgtactactc gaggttgtga
    42781 tgtttcctcc gaaatttcct caagtttttc cggtttctgg ccttttttgt tcatggcaat
    42841 cacttcttta gctctttgaa ctgaaatagt gataatgttg gcaccaaaat tttgagcagt
    42901 ggaataggtt atttgtccct tgaaaacatc tgttttgaaa tgataataag tattgtcaag
    42961 cgtttcgagc tgaatttctt tggatggaaa ttctttcatg gcttcctggt aggtatccag
    43021 ttcgtaattc atacagcact ttaatttgct gcattggccg gccagttttt gtggattaag
    43081 cgaaatatcc tggcagcggg ctgaactggt ggcaacagaa ttgaaacagg tcatccacga
    43141 agcgcagcac aattcccttc cacaagggcc aattcctcct atacgccctg cttcctgacg
    43201 ggcaccaatc tgtttcattt caatgcgcac gtgaaaagtt tctgcaaata cctttatcag
    43261 ttccctgaaa tctacccgtt cgtcggcgat gtagtaaaaa atggccttat ttccatcgcc
    43321 ctgaaattct acatctccaa ttttcatttt caggttcatc ctttcggcta tttgtctggc
    43381 ctgaatcatg gtagcgtgtt ccctgttttt ggcttcctga tatttttgaa ggtcggtatc
    43441 tttggctttt cgataaattc gcctgatttc catcttttcg ggatgaacat tgttcttttt
    43501 catttgcagc aataccagct tccctactag cgtaacttcg ccaatgtcgt gtcccggtga
    43561 tgattcgact gccactatgt cccctttctc aagaggaatt ttggtactgt tgagatagta
    43621 tccttttctg gtgtttttaa actgcacttc gacaaaatcg gtttctttct gcgactccgg
    43681 caaatcggcc atccagtcgt gaacgctaag tttcagattg ccgcatcggt tacagctttt
    43741 cttttttatt tggtaaccgc ctatatctaa tcgataatcc ataatttttg aatgataata
    43801 ttttattttc cggaagaccc aaatgaaaaa aagattttat tttgatcgat tatttcttta
    43861 aaggttttcc gtctgttcat aaattttatt ttttaagcaa tttagtgatt ttcaacgcca
    43921 aatcaaaaaa aaccattttt gaattgacgt tttgttcgat gtgccgttct gctaacgcaa
    43981 actcattcat catgtcttcg gcattggttt cattgataaa tggagcaaat ccgactgaaa
    44041 attctttttc aaacgaagtc ataaaattca gttcgggctg atgcagattg aaaatgaaat
    44101 tttcgcgaat catgcgttgt gcataactca aaaatttttt ctgtccttct ctgcccactg
    44161 atgcggcagc catttcttcg gaccattgcc ggattttttt caatgatgca taatcgcctt
    44221 tgttgccaat tatccatgct aaacgcataa tatttacaaa atgttcaaaa ttttccctgt
    44281 tcacgttgtt ctcgtctatc agagccaaag ctttcagaaa acttccttcc gaaatttgag
    44341 ctgcattggc cgcttcattt tcagaaatct gataacggct aacaagtgct tcagcaattt
    44401 cctcattgct taagcgggga attttaacct gttgcgtgcg agataaaatg gtggcaataa
    44461 tttcttcggg agaatttgaa accaaaagaa aaacggtgtt ttcgggcggt tcttccagta
    44521 tcttcagaag cttattggca cattggtcgt tcatcctctc gggcaaccag ataagcataa
    44581 ctttgtacct tgattcataa gtcatcaggt taacttttcg aataatttcg tctccctcgc
    44641 tggtataaat cattccttgc ttggcttcgc cggaaatttt ctggtaccag tcgttttggc
    44701 tgaaataaat acgttctgca aatatttgcc tgaaactgtc cataaaatca tcacaaacca
    44761 gtaatttttt tttctccgat ttgataatag ggaagacaaa atgcaagtcg ggatgcgcca
    44821 gtttaccgaa ttgaacacaa gacggacaaa ctccacacgc atcgttttcg agcggatgtt
    44881 cgcaacagat gtattgagca taggctattg ccaacggaag ttttccaatc ccttcaggtc
    44941 catagaatag ttgggcatga ggaattttct gttccttcac cgtgcgaatc aatcgctttt
    45001 ttacttcttc ttgtccgata acttgagaaa acagcatgac ttgtcggaaa aattattctc
    45061 tatattcatg caaatttacg agataatttt gaaattttaa atccagcaaa gaaaataaag
    45121 actttattcg tagagaatga aattcaggtt ccttgagaca gatgaaaatg ttttaatcgt
    45181 aagaattcct cgatgcttgc ggcgaggata gtcaatttta aggaatttct ttatttcatg
    45241 tagtttgggt ggataaaagt atcttgctca tattatttcg ctcagaaaaa atttgttttt
    45301 gcaaaatttt aaaacatcgc aaaccactaa aatgttgtaa atttgcatga ataaaatctt
    45361 tttcaataaa tttttaatgg acaagttttt ttgatagtaa aagaaggcag attatcatga
    45421 aaaattgaat tttttcgaca acaaacaaaa tctcaaattt tctcaaacta aataaggttg
    45481 ttatgttttt tgtcgaaaat aaaacaaatc aaaaaacgtt aaataaaata tctaattatc
    45541 aattgtttgc aaaaaaccat aaaatatggc accaaaaaaa ccagtgaaaa acggcggacg
    45601 aacgacttcc ggcaaagttc agaagaaatc ggagaaaaaa tcttccggca gcggttttgg
    45661 taagaaaatt gcaagttttt ttaccgacga acgtacacat ttcatagtcg gattcatgct
    45721 tttgctggta atgttgtacc tgatgttggc ttttgtatcc tattttttta cgggcgcagc
    45781 cgaccaaagc aaaatcgatt tgtcgtggag cgagttgcat gataagcgaa ctttaattca
    45841 gaactggact tccgttaccg gagccattgc tgccgagcat ctcatcaatc gttgttttgg
    45901 cgtatcagct ttctttattc cgattttcgg catttgggtg gctttacgct taatgaaagc
    45961 gcatgaaaaa tctgtttgga aggcattttt tcactgtgca ttttggcttg tgtggctttc
    46021 ggtaacgctc ggactgacgc tttcgcctta tttttccgat gttttgtttt tttcgttggc
    46081 gggcggacat ggcgattatg cagcacagtg gctggtttcc tatattggtg catcgggttc
    46141 gtttctgctt atggggggaa gttttatcgt ttatctggtc atagcttctg cctcgtcgct
    46201 tcctttcttt cgaaaacttt tcggaaagaa aaatgtatcc catacatctt ttgaagaaac
    46261 tctcgtagag ccggcagtgg cagtgggtac ggaaattgtt cccgaagaat ggatttcatc
    46321 aggcatagag actgaagtgt acgaagaatt ctcaaaaact caggaagaat cgcaaaccga
    46381 aaaagtggct tttgaggtgg aaacgcccga aaacaacgaa gaaagcaagc cggtggattt
    46441 ggatggcaac ggagaaaatg aaagtttgca ggaagatccc tattacaatc ttgccaaatt
    46501 gggaaaatat gaccctacac tcgatttgtc gcactatcat ttccctacgc tcgatttgct
    46561 caaagtttac ggtaccgaca acgaaacgcc catcgacatg gcggaacaga ttgccaacaa
    46621 aaaccgcatt atcaacacct tgctgaattt cgatatcgaa atagaaacca tcaaagcaac
    46681 ggtcgggcca accattacac tctacgaaat tgtaccgaaa gcaggagtgc gcatttccaa
    46741 aatccgaaac ctcgaatcgg acattatgct cagcctggcc gctaccggta ttcgtattat
    46801 tgctcccatt ccgggcaaag gcactattgg aatagaagta cccaacaacg atccgcaaat
    46861 tgtttcgatg cattccatca ttgcttctaa aaaatttcag gaatcgaaat ttgaacttcc
    46921 cattgctttc ggccgtacca ttaccaacga aattttcatg gtcgatttgg taaaaatgcc
    46981 acacctgctc gtagccggag ctaccggaca gggaaaatcg gtaggactga atgccattat
    47041 cacatcgttg ctttacaaaa aacatccctc cgaactgaaa ctggtgctgg tggatcccaa
    47101 aaaagtggaa ttcaatattt atgccggcat cgaaaaacat tttctggcaa aactgcccga
    47161 tggcagcgaa gccatcataa ccgatacaag caaagtggtc gaaacgctga attctctttg
    47221 cggagaaatg gatatgcgtt acgatttgct gaaaaaggcg catgccagaa acataaagga
    47281 atataacgag aagtttattt tgcgtcattt gaatccggag aaaggacacc gtttcctgcc
    47341 ctacattgtg gtgatcattg acgaatttgg cgatctgatc atgacggcag ggaaggaagt
    47401 tgaaatgccc attgcgcgaa ttgcacagct ggctcgtgct gtcggcattc atatgattat
    47461 tgccacacag cgtccttcgg tcaatatcat taccggaatt atcaaagcca atttcccggc
    47521 gcgggttgct tttcgcgttt cgtcgatgat ggattcgcga accattctcg atgctcccgg
    47581 tgccaatcag ctggttggac gtggcgacat gctgttttcg cagggaaacg acctgatacg
    47641 tgttcaatgt gcttttgtcg atactccgga agtggaaaat atcgttcatt ttatcagcga
    47701 acagcagagt tatcctactg ctttctattt gcccgaatat gtgaaaccag aaggagaaat
    47761 gggcgtagcg tctgccgact tgagcaaacg tgatccactt ttcgaggaag cggcccgact
    47821 tatcgttgct aatcagcagg gctctacttc gttgattcag cgaaaatttt ccattggcta
    47881 caatcgtgcc ggaagaattg tcgaccagct cgaaattgcc ggcattatcg gcccaaacga
    47941 agggagcaag gctcgtcagg tacttattcc cgacgaatat catttggaac agattttgaa
    48001 acaactttga tggctcaaca aaaaagcttg agataatctt tttaattatc aacaaattta
    48061 aaattttaac tttttccggt cgatatttcc ggatttaatc aacttaaatc accactaaaa
    48121 aattctgatt atgcagtttt catttcataa cccaagtcgt tgggtaaaat cattcattta
    48181 ttctatgctg ttatcgataa tttcgataca acccgctctt tcacagaatg atcctgaagc
    48241 tcaaagaatt atagacagtt ttttgaatac cgcacgcacc ggtgccatta ctacgcgctt
    48301 taccgtttcg gtaatcgaaa aaaacggcgt aaattcgtac acgaattccg gaacatttac
    48361 cttaaagggt aaccgtttcg tattggagat gcaggaaatg aaagtgtggt ttgacggaaa
    48421 gacacaatgg gcttaccaga aatcaggcaa tgaagttacc attaccgagc cgtcagagca
    48481 ggagttggct caaaccaatc cgatggcaat tcttggtaat tatcggaaca agtgcaatat
    48541 ccgtttctgc aagacttctt cggagtattg gtgcattcag ctgacaccca aatcgataaa
    48601 gaatgaagat attgcggaaa ttgaagtaca atttctcaag acagacggaa taccgcaatc
    48661 cataacagta tctcaaaaaa acggaacaag aacagttctg atgcttagtc agtttcagaa
    48721 aaatgccaaa gtttcggaac agatattcac gtttgatgca aaaaaataca aagacgccta
    48781 tatcaacgat ttgagataaa aatttttaaa aaactacaaa aagaaatgac aactaacgaa
    48841 aagatacgat gcctgatcat aggatcggga ccggcaggtt acactgctgc catttatgct
    48901 tcaagggcca atctttcgcc cgtactttat cagggtatgg aaccgggagg tcagttgaca
    48961 acaaccaacg aagtggaaaa ttttccgggt ttccccgaag gaattaccgg acctgagttg
    49021 atggacaaaa tgcagaaaca ggctgaacgt tttggtgcgg atattcgctt cggaatggtt
    49081 acgtcaaccg atttgtcggc ttctccctat cgcgttacca tcgataacga aaaaattatt
    49141 gaagcccaaa gcattattat tgcaaccggt gctacggcaa aatatcttgg attgcctgac
    49201 gagcagaaat atgccggttc gggcgtatcg gcctgtgcca cgtgcgatgg atttttttac
    49261 agaaatcggc cggtagcagt agtgggaggt ggcgatacag cttgtgagga agctctttat
    49321 ttagccaata ttgcttccaa agtctatatg attgtgcgca agccatttct cagagcttcg
    49381 aaaataatgc aggaacgcgt tttgcaacat ccgaaaatcg aagtcctttt tgagcacgaa
    49441 acactcggat tgacaggcga aaaagtggtg gaaggggctg atttggtgaa acgtcgcgga
    49501 caacccgacg aagaaaaagt gcacatcgcc attgacggtt tttttctggc tatcgggcac
    49561 aaacccaatt cggaagtatt taaaccctgg attgaaaccg acgaaaacgg atatatcaaa
    49621 actattcccg gtacaccacg aaccaatgta gaaggagttt ttgcagctgg tgatgttgct
    49681 gatcctcatt accgacaagc cgtgtcggct gccggttcag gctgtcaggc agctatcgaa
    49741 gctgaaagat atttggtgga taaaaaataa aagttagtaa agcagtttta attttgctgt
    49801 cggaaattaa gttgaatgag ggagttaata ctgtttttcc ctgcacaaat ttatatgctg
    49861 tgaagttgtt tttctgtcaa tgaaaaataa ttcttaacac tattttcaaa taaaatgaaa
    49921 cccacatgta ttacatacgg ttgcggtata tgaaaagttg ggcatttcgg agcaattaac
    49981 tgtcaaaccg cgcaaagtta aatgcgagtt aaacacctca aatttagcac tatctgccca
    50041 attttttata taccgtgtta tgcactggcg gttcttgtct gtttgtatgt tagagtcaaa
    50101 ttgcactttc atgagaatat aatttatttt ttaaaggtct gatggaaccc acatgtctaa
    50161 atatttatat tcattcgtgt ttgtgtatgc aatacatgtg ggtttcatgt tgttgctttt
    50221 gcttatgagt caatggcaaa aaccatgttg aatacttact ttgaaatgta tccacaatta
    50281 tttcttttta tttttataat taatcttttt ctccgcagca atttttaccg atttttcctc
    50341 aattgaatat tcctttgttt tcttgccctt gccctgcgct tgccgtatga gtttcttttc
    50401 tgctgccaat ttctctttct cggcttttat tctttcgagc aatacacttg ccggctcgtc
    50461 gtttgggtct tgtgccacca gctcgccacg aaatgctttg gctaaaatac tttggggtag
    50521 tttatccaac atggctttgg ctttggtgta acgtgcttct attttatcgg c
//
LOCUS       DAOTAX010000006        48599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_591_length_48599_cov_30.276636, whole genome shotgun sequence.
ACCESSION   DAOTAX010000006 DAOTAX010000000
VERSION     DAOTAX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 48599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48599
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_591_length_48599_cov_30.276636"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLM04_01275"
     CDS             complement(<1..205)
                     /locus_tag="PLM04_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01745.1"
                     /translation="MKNIKKFSLFFLFIGLIGASCSTDEENTVVPKTLEQYKQDFSQF
                     VSAEKTKVQNCKMGYNKGDFKYSS"
     gene            complement(233..1771)
                     /locus_tag="PLM04_01280"
     CDS             complement(233..1771)
                     /locus_tag="PLM04_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HON01746.1"
                     /translation="MKNKIFITAISLCGFFLTSCEDFLTDKPESVLTQVDFYTTPIRI
                     NQGVIGCYAGLANIQKDEWMYTELRSDNSCQSETGSSATIRIDQTDIASFRLSPSLPA
                     LRDYWYKIFQNISNINAVLPSVRDNSYVANESLRAQYEGELLFLRAYHYYTLVNLFGD
                     MFKITKVIGPNEAKKIPRSPVSEIYNEIIIPDLKKAVEELPSTYSATDLGRATKWAAK
                     GMLAKAYMMLGGKDNLSLAKKELEDILTLSPHGLLTGTGAYANIFSISNEMNKEIIFA
                     VRYKGGSSGIGSPFWGTFAPLGSANLFLKIGTPLGYNNPTYELMNLFNANPNDTRKDA
                     CFKVWNKSSTVSVPYISKYMDASMTQPLQSENDWIILRFADIKLLYAEILAQDGNFAN
                     AHNPLNDVRARAGLDGIGPFGSKEEALDAVYAERRLELAFENQRWFDLLRMAKSYDNP
                     DKPMEILRRHTFETDWAVLYSKYNPMQPPEERFYIKERLLLPIPQAEIDTNNEMVIPQ
                     NPTY"
     gene            complement(1816..5055)
                     /locus_tag="PLM04_01285"
     CDS             complement(1816..5055)
                     /locus_tag="PLM04_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON01747.1"
                     /translation="MKRTKKPKFLKREVFVLIALFISTISAFSQIKVTGTVLDTKKEP
                     LIGVNITVKGSRTGTISDFNGKFNIDVPDRQSVLVFSYIGYKTIERTIPASGQLTVIL
                     EEETQTIDEVVVVAYGTQKKSHLTGAVSSLKNEKMDEIPVSRVDQALQGKIAGVQINN
                     VNPEAGAAPKIRVRGLGSISASSDPLIVIDGFPVPDGLSMVSMADVQSVEVLKDASSA
                     ALYGSRAAGGVILITTKSGEINKPKYNFKMYSGFRNPLKLPDMLSTEEYVALLYREAD
                     MRKLDPAVNGIATETMAFNKITEPEKAGYLIMKYLDDQPTDWLNEGLRDRGTMQNYQL
                     SASGGDKNLKYFVSGNYVNEDGIMLNSTYDKFTFRAKLDAKLSKAATLGVNLSPTYSR
                     QERPAVDLTDYMRFPSWLPIRHNEATAALTGKVAGDYAQPSDFNGINISGIGYNNEVW
                     SLPGVTVWNSSNQNPVSIRERTSIFTDDYRFQGNIYLTLNLLPGLQFKTSNGAYLLFR
                     EYNKKEQTSANKAGNPNSLTRQLTTHRELLSENTLNYNKKISNHEFDALLGFTMQQTN
                     DNYNRIVGTNFPDEQILSFNLASQILLDSPSIDGTTSFYYTESLVSLLGRLNYAYLGK
                     YMMSASFRADGSSKFYKGNKWGTFPAFSAGWRASEEKFMKNFEWLSNLKLRVSYGLTG
                     NNNIPQYAYMNSINTSNYVLGSGNGTLVQGMVSNSSSLGNPDITWEQTSEANYGIDFG
                     FLNSRLNFSIEYYNSNTIQLLLQQPAMYITGHQSYWNNIGKVNNKGIEIELNTTNIAN
                     KKFTWKTSANFSTNKNKLLNYGDKEYEDNFGERSEVYRAIVGEPAIQYYGYKTDGVYT
                     TFEEVAAAKALKDENGNPFTYTKFAPIVGGIKVVNINGDNKIDTEDRTVLGDPFPDFT
                     WGITNTFTYKNFDLSFLIQGVQGIDIINGNVNYNEQLRYNKAYTNNRYVSPMFPGDGK
                     TVYSNTTSGSDLMLTDYCIEDGSYGAIRDFTLGYTLPQNLLKSLKLRNLRVYFSAENL
                     VYIMAPSYRGINPEARRTASNYTSPLVDGYQRGAFPLNRTFTAGLDINF"
     gene            complement(5368..6111)
                     /locus_tag="PLM04_01290"
     CDS             complement(5368..6111)
                     /locus_tag="PLM04_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HON01748.1"
                     /translation="MMEDNFIRLMKAQQKSPADEVINKIKELIESGELKPGDRLPAER
                     KLASDFGFGRTHIREALQKLEFYGIVKTLPQSGTILNGLDINSLNGLLSDVLNLQSYD
                     FYSLVETRCILEMNAIRLCAERRTEEDIEAMEKSHQNFIKFHNDEILRVSYDFAFHRN
                     IAEGSHNPVIKSMLMTIIPDILIIYNKERVCNTDPFVVIKEHAEMINAVKMQNGELAS
                     SLLYQHVQGVMTLARTKLENLKVKSLSMA"
     gene            6450..7745
                     /locus_tag="PLM04_01295"
     CDS             6450..7745
                     /locus_tag="PLM04_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HON01749.1"
                     /translation="MQPSEIQAVKQRFGIIGNSELLNRAIDVAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHYYSHRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAMADRKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLETGEFIKVGSSKIQKTNVRVIAATNLNMT
                     QAVRDGRFREDLYYRLNTVPIYVPPLRERKEDIVLLFRKFAADFAEKYHMPPIKLTED
                     AKKLLTEYYWNGNVRQLKNITEQISVIEQQREITAEILKQYLPKDELERFPVLLSNHR
                     SDQKTFTSEREILYQVLFDMKQDMIELKKLVYNLMNAQGNQELLQKDFPLSPSYVAPH
                     ETFVPATPVPVDVLKPVNNLGREVNQFPNTNKQNSFEEAAEFQEIEDIQEPMTLEDME
                     RQMIKKTLEKHKGKRKAAAEELKISERTLYRKIKEYGIE"
     gene            7732..8292
                     /locus_tag="PLM04_01300"
     CDS             7732..8292
                     /locus_tag="PLM04_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HON01750.1"
                     /translation="MELNRKPKNSFFGRNTKSVKLLSAIFCGVFLLLASCTVSYKFNG
                     ASIDYTKIKTISIADFPNVSELVYPPLSQQFSEALRDIYTKQTQLRLVRQNGDLNLEG
                     EIVGYQLTPMSISADTYASETKLTVTVKVRFTNKVNPADDFEKTYSAFQVFDSSKMLN
                     DVQEELVKAIITEITENIYNDTVAKW"
     gene            8332..8988
                     /locus_tag="PLM04_01305"
     CDS             8332..8988
                     /locus_tag="PLM04_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01751.1"
                     /translation="MTQAEFFTLLQHPESITSEHTPDLKDLVEHYPYFSQARMLYLKA
                     LQVSGSIHFEKTIKTAVVYTSQRRWLYHFLYPEVFAKEVYRRPEKGKLTGSYFDLLDV
                     VEKEGGDSGQTLKNLADQLKKARAMVMENPPAPDEEEPVKKKESTKSPALQSEYFAEE
                     IIPELEISEESVKRLIKNRKYTEALEILKKLNLNNPKKSVYFADQIRFLEKVIENLKI
                     "
     gene            9002..9421
                     /gene="secG"
                     /locus_tag="PLM04_01310"
     CDS             9002..9421
                     /gene="secG"
                     /locus_tag="PLM04_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HON01752.1"
                     /translation="MYLLFTILIIIASILLILIVLVQNSKGGGLAAGFSSSNQYMGVR
                     KTADFLEKATWGLIAFIVVLSIGATGIHANQKSGGSDSEIKEQYQKAQEELPGVVAPD
                     FGETQGAGNNQQDNNGGQQNNGNQQKAPQKLPVVPAK"
     gene            complement(9552..10016)
                     /locus_tag="PLM04_01315"
     CDS             complement(9552..10016)
                     /locus_tag="PLM04_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01753.1"
                     /translation="MKEKLIVLLLASASFMGLNAQVRGQALGVRLGYGGEVSYQHPLN
                     NQNRIEGDLGFYYDHGFNLAGVYQWVFDLSELSPGFNWYVGLGPQIGSWSNQDNKDYD
                     GLALGIVGQIGIEFNFNIPLQLSLDYRPGWYVLPSSYGGAWDGIALGVRYNF"
     gene            complement(10070..>11689)
                     /locus_tag="PLM04_01320"
     CDS             complement(10070..>11689)
                     /locus_tag="PLM04_01320"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HON01754.1"
                     /translation="ELVRFTTQGNREAESLKKIHDLLRDKGYQTPLSADVHFNANVAD
                     VVAQYVEKVRINPGNYLGSNRGNITSDYNDEEYKAEYERIRKRFKQLLNICNKHQTAL
                     RIGVNHGSLSERIMNRYGDTAEGMVESCMEFLRICREENFHNVVISMKASNTLTMIHS
                     VRMLVERMEAENFHFPLHLGVTEAGNEEEGRIKSAVGIGTLLSEGIGDTIRVSLSEDP
                     EEEIPVARKLISYIDTKKNHEPIIAEKNFQHSPFNYHRRTTHAVENIGGNNVPVVIAH
                     TKENFEITPDYYFAENQIINIHNEVFPIFDIDEKEKIIKHSSRIKFLKMSFPELTKEN
                     VHFLKNTADTVVILESHHKNAPAEQKAFFHSLMNAGCTVPVIVTQSYKENTEEDLEIK
                     AGVDFGMLFVEGFGDGIFLKNEGTIPSEKLNSIAFGILQATRARISKTEFISCPGCGR
                     TMFDLQTVVSRIKSKTSHLKGLKIGIMGCIVNGIGEMADADYGYVGAGRGKVSLYKKK
                     ICVEKNIPEEEAVEKLIELIKINGDWTEPIS"
     assembly_gap    11690..11789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11911..12207
                     /locus_tag="PLM04_01325"
     CDS             11911..12207
                     /locus_tag="PLM04_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01755.1"
                     /translation="MTHSYDDLVATFESNLRKLMEDFKSLQEENSLLRAQLSNKEQDI
                     ELLKVKFSELQKNYDHLRIAKTLSLSDEDRIKSKQQINKLVREIDKCLALLLND"
     gene            12215..12511
                     /locus_tag="PLM04_01330"
     CDS             12215..12511
                     /locus_tag="PLM04_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HON01756.1"
                     /translation="MNENDTFLINVQIGGYRLPLRIERKEEELYRLAEKVLGKYLEVY
                     QKKYSHRSAEEVFIFVAYELAVMLVRKMMQQDTAPLAEKVSSLNQELEKLLAEK"
     gene            12665..14185
                     /gene="rny"
                     /locus_tag="PLM04_01335"
     CDS             12665..14185
                     /gene="rny"
                     /locus_tag="PLM04_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HON01757.1"
                     /translation="MNLIIAIIISFGVGVGGSFIYFKRNHKKALKELEAEAELIKKNN
                     LIEVKEKFIALKAEHEQQVQQRNAKIQAAETRIQQREMQLNQKQGELQRKMNEVDVMK
                     ENLESQFLTVENKKKELEHLIRQAQEQLETVSGLSSEQAKERLIESLKEEAKTEAMAY
                     VNDIMDEAKMTANKEAKRIVIQSIQRLATETAIENSVTVFHIESDEIKGRIIGREGRN
                     IRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEVVNK
                     VRKQLEEEIIEVGKRTTIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSRETANLCA
                     TMAAELGLNVKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPDICNAIGAH
                     HDEIEMESLIAPIVQVCDAISGARPGARREIVEAYIKRLKDLEDLALSYPGVLKTYAI
                     QAGRELRVIVGADKIDDKEAELLSFDIAKRIQDEMTYPGQVKITVIRETRAVSYAK"
     gene            14202..14601
                     /gene="ssrA"
                     /locus_tag="PLM04_01340"
     tmRNA           14202..14601
                     /gene="ssrA"
                     /locus_tag="PLM04_01340"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14862..16655
                     /gene="argS"
                     /locus_tag="PLM04_01345"
     CDS             14862..16655
                     /gene="argS"
                     /locus_tag="PLM04_01345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HON01758.1"
                     /translation="MNLEHTIQQLVEQALQQLYGLEIGQNPLQVQKTRKEFEGDMTVV
                     LFPFIKLLRKSPEQIGKEIGDNLLKSSSVIEAYNVVKGYLNLKISVPYWLERLNEIFL
                     QKDFWKVKLKENADLMMIEYSSPNTNKPLHLGHVRNNLLGWSIAQIQEANGWKVVKTN
                     LVNDRGIHICKSMLAWKLFGNGETPQSSGLKGDHLVGKYYVLFDQAYKQQVRELVEEG
                     WSEEEAKKSAPLMQEAQKMLLAWENNDPEVRKLWETMNGWVYEGFNETYRKLGIDFDK
                     IYYESETYLVGKKIVEEGLDKGIFYRRPDGSVWADLRDDGLDEKLLLRADGTSVYMTQ
                     DIGTAKVRYDDFPIKKMVYVVGNEQNYHFQVLSILLDKLGFEWGKSLVHFSYGMVELP
                     DGKMKSREGTVVDADDLIDEMLRTARETSEELGKLDGCSPEEVENIVRMVALGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTHARIRSVLRKAQEQSISYGKLNNLNLS
                     ISEKERYLVQLLTEFPLMVKEAGDEFNPALIANFLYELVKEYNQFYHDYSILKEENTD
                     LRNFRLVLSSAVADVIKDGMALLGIEVPERM"
     gene            16959..17369
                     /gene="rpsL"
                     /locus_tag="PLM04_01350"
     CDS             16959..17369
                     /gene="rpsL"
                     /locus_tag="PLM04_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HON01759.1"
                     /translation="MPTIQQLVRKGRTELVDKSKSPALDSCPQRRGVCIRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRLQRRSKYGAKRPKKGQPAAASTGKKK"
     gene            17561..18037
                     /gene="rpsG"
                     /locus_tag="PLM04_01355"
     CDS             17561..18037
                     /gene="rpsG"
                     /locus_tag="PLM04_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HON01760.1"
                     /translation="MRKSKPKRRVVLPDPIFNDPMVTKFVNHLMFDGKKSIAFDIFYS
                     ALENVKNKLPNEEKSSLEIWKKALENVTPLVEVRSRRVGGATFQVPTEIRPERKESIS
                     MKNLILYARKRGGHSMSDKLAAEIVDAFNSQGGAYKRKEDIHRMAEANRAFAHFKF"
     gene            18057..20180
                     /gene="fusA"
                     /locus_tag="PLM04_01360"
     CDS             18057..20180
                     /gene="fusA"
                     /locus_tag="PLM04_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HON01761.1"
                     /translation="MAKVDLSYTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTYWNYNQHQYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVATFCAVGGVEPQSETVWRQADKYNVPRLCFVNKMDRSGADFFEVVRQIREVLGA
                     KPCPIQIPIGAEEKFKGVVDLIKMKAIFWHDETMGAEYSEEEIPADLVNDAREWREKM
                     LEIVAEYDDAVMEKFFDDPSKITEDEIKAAIRKGTLAKEINPVICGSAFKNKGIQTML
                     DAVCAYLPSPLDNPEIVGKDPRYEDKTVVRHPDPNEPLCALAFKIATDPYVGRLCYFR
                     VYSGKLESGSYVYNPRTDKKERISRIFQMHSNKQNPVDMISAGDIGAGVGFKDIKTGD
                     TLCDENHPIVLESMDFPEPVIGISIEPKTQKDLDKLGVGLSKLAEEDPTFTVHTDEQS
                     GQTIISGMGELHLEIIIDRLRREFKVECNQGRPQVAYKEAITQPVEIREVYKKQTGGR
                     GKFADIIVRVEPADNDFEGSLQFIDMVKGGNIPKEYIPSIQKGFQSAMKNGVLAGFPV
                     DRLKVTVLDGSFHPVDSDQLSFEICAQIAFKNACTKAKPVLLEPIMKIEVVTPEESMG
                     DVIADLNKRRGQVEGMESSRTGARIIKAKVPLAEMFGYVTALRTITSGRATSSMEFSH
                     YAEVSNSLAREVVAEAKGKVELLHY"
     gene            20250..20555
                     /gene="rpsJ"
                     /locus_tag="PLM04_01365"
     CDS             20250..20555
                     /gene="rpsJ"
                     /locus_tag="PLM04_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HON01762.1"
                     /translation="MSQKIRIKLKSYDYNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            20675..21292
                     /gene="rplC"
                     /locus_tag="PLM04_01370"
     CDS             20675..21292
                     /gene="rplC"
                     /locus_tag="PLM04_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HON01763.1"
                     /translation="MPGLIGKKIGMTSVFSADGKNVPCTVIEAGPCVVTQIKSIETDG
                     YEAVQVGFEEKKPKHTNKPEAGHFKVAGVAPQRHLVEFKGFEGDFKLGDVITVEIFDG
                     STFVDVVGTSKGKGYQGVVKRHKFSGVGETTHGQHNRQRAPGSLGASSFPSRVFKGMR
                     MGGRMGGDKVTVQNLQVLKIIPEHNLLLVKGSVPGAKGSIVIINK"
     gene            21295..21927
                     /gene="rplD"
                     /locus_tag="PLM04_01375"
     CDS             21295..21927
                     /gene="rplD"
                     /locus_tag="PLM04_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HON01764.1"
                     /translation="MELSVLNIKGEDTGRKVSLNEAIFGIEPNDHAIYLDVKQFLANR
                     RQGTHKAKARSEISGSTRKLGRQKGGGGARPGDIKSPLRVGGGRVFGPVPRDYSFKLN
                     KKVKQLARKSALSYKAKNGAITVVENFEFEAPKTKKMLELAKNLQVADKKPLIVLSSA
                     NKNVYLSARNLPSIDVITTSELNTYNVLNAKTLVLTEGSVTILEQIFKVE"
     gene            21942..22232
                     /gene="rplW"
                     /locus_tag="PLM04_01380"
     CDS             21942..22232
                     /gene="rplW"
                     /locus_tag="PLM04_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HON01765.1"
                     /translation="MGVILKPLVTEKLTKLGDKFNRYGFRVQKDANKLQIKQAVETMY
                     NVTVVDVNTMIVPSKKRSRYTKSGVISGKRPAYKKAIVTVKKGEEIDFFSNI"
     gene            22242..23066
                     /gene="rplB"
                     /locus_tag="PLM04_01385"
     CDS             22242..23066
                     /gene="rplB"
                     /locus_tag="PLM04_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HON01766.1"
                     /translation="MAVRKLLPTTPGQRNKIISKFDTITKSEPEKSLVYGKRKTGGRN
                     NNGKMTMRYIGGGHKKKFRVIDFTRKKDGIPATVKAIEYDPNRSARIALLYYADGEKS
                     YILAPNGLQVGQKIISGSDVAPEVGNALPLENIPLGTIVHNIELRPGQGAAIARSAGT
                     FAQLVSREDKYVIIKLPSGEVRKILGACKATVGSVGNSDHALEKSGKAGRSRWLGRRP
                     RVRGVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRHPKKQSSKYIIERRKK"
     gene            23089..23355
                     /gene="rpsS"
                     /locus_tag="PLM04_01390"
     CDS             23089..23355
                     /gene="rpsS"
                     /locus_tag="PLM04_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HON01767.1"
                     /translation="MSRSLKKGPYISLKLEKKILDMNESGKKTVVKTWARASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFALTRTFRGHAGKKK"
     gene            23399..23812
                     /gene="rplV"
                     /locus_tag="PLM04_01395"
     CDS             23399..23812
                     /gene="rplV"
                     /locus_tag="PLM04_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HON01768.1"
                     /translation="MGARKKISADRRKEARQTLYYAKLNDVPTSPRKMRLVADMIRGM
                     EVNKALSVLKFSSKEASRRMEKLLKSAISNWEIKTEQKADNANLFVSKVYVNEGTTLK
                     RLRTAPQGRGYRIRKRSNHVTLYVDSRNNNDTNQI"
     gene            23821..24573
                     /gene="rpsC"
                     /locus_tag="PLM04_01400"
     CDS             23821..24573
                     /gene="rpsC"
                     /locus_tag="PLM04_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HON01769.1"
                     /translation="MGQKTNPISNRLGIIRGWDSNWYGGKKYGDTLLEDSKIRKYLNV
                     RLSKASVSRIIIERTLKLVTITVCTARPGIIIGKGGQEVDKLKEELKKITDKEIQINI
                     FEIKRPELDAVIVASNIAHQLEGKIAYRRAVKMAIASTMRMGAEGIKVQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYACAEALTKVGLIGIKVWICRGEVYGKRDLAPSYVT
                     GQKEGSRTERRSGDHHSGGDHGNKNSSRRKKK"
     gene            24598..25032
                     /gene="rplP"
                     /locus_tag="PLM04_01405"
     CDS             24598..25032
                     /gene="rplP"
                     /locus_tag="PLM04_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HON01770.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKSMDTVWLTG
                     QQIEAARIAVTRYMQRQGQVWIRVFPDKPITKKPAEVRMGKGKGSPEGFVAPITPGRI
                     IIEVDGVPYAVAKEALRLGAQKLPIITKFVVRRDYDLTDQNL"
     gene            25036..25230
                     /gene="rpmC"
                     /locus_tag="PLM04_01410"
     CDS             25036..25230
                     /gene="rpmC"
                     /locus_tag="PLM04_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HON01771.1"
                     /translation="MKTREIRELSTKELLERLETEKENLVRLKLNHSISTLDNPMQIK
                     VVRRNIARIATELRLREINQ"
     gene            25245..25502
                     /gene="rpsQ"
                     /locus_tag="PLM04_01415"
     CDS             25245..25502
                     /gene="rpsQ"
                     /locus_tag="PLM04_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HON01772.1"
                     /translation="METRNLRKERIGVVTSNKMDKTITVAVRWKEKHPIYGKFVNKTK
                     KYYAHDEKNECNIGDTVSIMETRPLSKLKRWRLTEIIERAK"
     gene            25505..25870
                     /gene="rplN"
                     /locus_tag="PLM04_01420"
     CDS             25505..25870
                     /gene="rplN"
                     /locus_tag="PLM04_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HON01773.1"
                     /translation="MIQQETRLLVADNSGAKEALCIRVLGGTGKRYATVGDTIVVSIK
                     SVIPSSDIKKGMVSKAVVVRTKKEVRRVDGSYIRFDDNACVLLNNAGELRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
     gene            25893..26204
                     /gene="rplX"
                     /locus_tag="PLM04_01425"
     CDS             25893..26204
                     /gene="rplX"
                     /locus_tag="PLM04_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HON01774.1"
                     /translation="MTKLHIKKGDIVYVNTGVDKGKTGRVLEVLVKEQRAIVEGINLV
                     SKNMKPNAKYPQGGIVKQEAPIHISNLNPVENGRPVRVGRKLNNEGKLVRISKKTGEE
                     I"
     gene            26207..26752
                     /gene="rplE"
                     /locus_tag="PLM04_01430"
     CDS             26207..26752
                     /gene="rplE"
                     /locus_tag="PLM04_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HON01775.1"
                     /translation="MNTASLKQEYKERIVPALMKEFAYKSVMQVPRLEKIVLNQGLGI
                     AVADRKIIDTAINEMTTITGQKAVATVSKKDISNFKVRKKMPIGVRVTLRGDRMYEFL
                     ERLIRVALPRIRDFKGIENKLDGRGNYTLGITEQIIFPEINIDSISRLLGMNITFVTT
                     AKTDEEGFALLKEFGLPFKKN"
     gene            26766..27035
                     /gene="rpsN"
                     /locus_tag="PLM04_01435"
     CDS             26766..27035
                     /gene="rpsN"
                     /locus_tag="PLM04_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HON01776.1"
                     /translation="MAKESMKAREVKRNKMVAKYEAKRSLLLAEGNYEALQSIPKNAS
                     PVRLHNRCQLTGRPKGYIRQFGVSRIVFREMASKGLIPGVKKASW"
     gene            27156..27551
                     /gene="rpsH"
                     /locus_tag="PLM04_01440"
     CDS             27156..27551
                     /gene="rpsH"
                     /locus_tag="PLM04_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HON01777.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEIPASNLKREITRILFEQGYI
                     LNYKVVEDGPQGTIKIALKYDPVNKVNSIKKLIRVSKPGLRKYVGYQDIPRVLNGLGI
                     AILSTSSGVMTNKEAAKLKIGGEVLCYVY"
     gene            27570..28121
                     /gene="rplF"
                     /locus_tag="PLM04_01445"
     CDS             27570..28121
                     /gene="rplF"
                     /locus_tag="PLM04_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HON01778.1"
                     /translation="MSRIGKLPIKIPAGVNVTIKNNLVSVSGPKGTLSQQIHPNISVE
                     VKDGFCTLTRIDDEKENRAMHGLYRSLINNMVEGVSNGFKKTMELVGVGYRVSAQGQV
                     LEFALGYSHSIFLKVPPEVKVETKSERNQNPLIILESCDKQLLGQVCAKIRSFRKPEP
                     YKGKGIKFQGEVLRRKAGKSAGK"
     gene            28143..28484
                     /gene="rplR"
                     /locus_tag="PLM04_01450"
     CDS             28143..28484
                     /gene="rplR"
                     /locus_tag="PLM04_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HON01779.1"
                     /translation="MTKLDRRQKIKAHIRKKIFGTAQRPRMTVFRSNAQIYVQLIDDE
                     KGITLASASSLAIKEKITKQEQAGKVGALIAQAAKEAGITEVVFDRNGYLYHGRVKQL
                     ADAAREGGLKF"
     gene            28491..29009
                     /gene="rpsE"
                     /locus_tag="PLM04_01455"
     CDS             28491..29009
                     /gene="rpsE"
                     /locus_tag="PLM04_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HON01780.1"
                     /translation="MAKMMNRVKTTTDLELKDRLVAVNRVTKVTKGGRTFSFSAIVVV
                     GNEDGIVGWGLGKASEVTAAIAKGTEVAKKNLIKVPVLNGTIPHEQEAKFGGARVFIK
                     PASSGTGVTAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATIAALSELRDAYTVAQ
                     NRKVSLDKVFNG"
     gene            29022..29198
                     /gene="rpmD"
                     /locus_tag="PLM04_01460"
     CDS             29022..29198
                     /gene="rpmD"
                     /locus_tag="PLM04_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HON01781.1"
                     /translation="MATIKIKQVKSKIKSPKVQKLTLEALGLHKINDVVEHEATPQIL
                     GMVEKVKHLVKIVD"
     gene            29228..29677
                     /gene="rplO"
                     /locus_tag="PLM04_01465"
     CDS             29228..29677
                     /gene="rplO"
                     /locus_tag="PLM04_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HON01782.1"
                     /translation="MDLHNLTPAAGSVKSNKRKGRGSGSGMGGTSTRGHKGAKSRSGY
                     AHKIGFEGGQMPIQRRLPKFGFKNINKIEYKAINIDTLEILAQENQLSKIGIDDLRQA
                     GFISKNQLVKILGRGKLTVKLDVEAHAFSKSAENAIVGAGGTAVKIS"
     gene            29679..31022
                     /gene="secY"
                     /locus_tag="PLM04_01470"
     CDS             29679..31022
                     /gene="secY"
                     /locus_tag="PLM04_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HON01783.1"
                     /translation="MNKLIETLKNIWKIEDLRNRLLTTLLFVMIYRFGSYVVLPGIDA
                     GALTALKAQTSGGLLALLDMFSGGAFANASIFALGIMPYISASIVIQLLTIAVPYFQK
                     MQREGESGRRKMNQITRYLTVGILLLQGPSYLINLSVQLRQAGSALPSGFFFQATSTI
                     ILCAGSMFVMWLGERITDRGIGNGISFIILVGIIARLPQAIVREFASRLTDVGGGLIM
                     FIIEILFLLLVIGASIMLVQGTRKVPVQYAKRVVGNKQYGGVRQYIPLKINAAGVMPI
                     IFAQAIMFIPITIVGFSKSESMQGFVGAFMDNTGFWYNFVFAILIILFTYFYTAITIN
                     PTQMAEEMKRNNGFIPGVKPGKKTAEYLDMIMSRITLPGSIFLAIIAIMPAFASIFGV
                     NHAFAQFFGGTSLLILVGVTLDTLQQIESHLLMRHYDGLLKSGRIKGRTSGVSAY"
     gene            31040..31825
                     /gene="map"
                     /locus_tag="PLM04_01475"
     CDS             31040..31825
                     /gene="map"
                     /locus_tag="PLM04_01475"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HON01784.1"
                     /translation="MIYLKSEEEIELLRISNQLVAKTLAELAKHITPGISTLELDRIA
                     DEFIRDHGAVPSFLDYNGYPNSICTSVNEQIVHGIPSDKVFLKEGDIISIDCGAYING
                     FHGDSAYTFCVGEVDQQIVNLLKTTKESLYKGIEQAQEGHRTGDIGYAVQSYCEQRGY
                     SVVREMVGHGLGREIHEAPEVPNYGRKGNGVMLKYGMVICIEPMINMGTRHIVFEKDG
                     WTTRTLDRKPSAHFEHAVAIRQGKADILSSFEYIEQVLGERAI"
     gene            31831..32049
                     /gene="infA"
                     /locus_tag="PLM04_01480"
     CDS             31831..32049
                     /gene="infA"
                     /locus_tag="PLM04_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HON01785.1"
                     /translation="MAKQTAIEQDGTIIEALSNAMFRVELENGHIITAHISGKMRMNY
                     IKILPGDKVKVEISPYDLTKGRITFRYK"
     gene            32066..32182
                     /gene="ykgO"
                     /locus_tag="PLM04_01485"
     CDS             32066..32182
                     /gene="ykgO"
                     /locus_tag="PLM04_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HON01786.1"
                     /translation="MKVRASIKKRSEDCKIVRRKGRLYVINKKNPRFKQRQG"
     gene            32209..32589
                     /gene="rpsM"
                     /locus_tag="PLM04_01490"
     CDS             32209..32589
                     /gene="rpsM"
                     /locus_tag="PLM04_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HON01787.1"
                     /translation="MAIRIVGVDLPQNKRGEIALTYIFGIGRSSANKILSKAGVDRDI
                     KVKDWTDEQAAKIREIISSEYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRLGLPVRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            32638..33027
                     /gene="rpsK"
                     /locus_tag="PLM04_01495"
     CDS             32638..33027
                     /gene="rpsK"
                     /locus_tag="PLM04_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HON01788.1"
                     /translation="MAKKTVAAKKRVVKVDAAGQLHVHSSFNNIIISLTNENGEVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKIAFDLGLRKVKAYVKGPGNGRESAIRTVNG
                     IGIEITEIVDVTPLPHNGCRPPKRRRV"
     gene            33190..33795
                     /gene="rpsD"
                     /locus_tag="PLM04_01500"
     CDS             33190..33795
                     /gene="rpsD"
                     /locus_tag="PLM04_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HON01789.1"
                     /translation="MARYIGPKTKIARKFGEAIFGPDKVLSKKNYPPGQHGNNRRKKT
                     SEYGIQLREKQKAKYTYGVLEKQFHIIYERARRASGVTGVILLQMLESRLDNLVFRMG
                     IAPTRAAARQLVNHKHITVDGKVVNIPSYQVKPGQVVGVREKDKSMEVIQNSLSGFNH
                     SKYPWIEWNASTLSGTYLHIPEREDIPENIKEHLIVELYSK"
     gene            33807..34799
                     /locus_tag="PLM04_01505"
     CDS             33807..34799
                     /locus_tag="PLM04_01505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HON01790.1"
                     /translation="MSILAFQKPEKVIMLEADDFHGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAITSIKIEGIDHEYSTIPGVIDDVTNIILNLKKVRFKQVVEGVDNEKVSI
                     TVSDTAVFKAGDIGKHLTGFEVLNPELIICELDPKGSFQIDIAINKGRGYVPAEENRT
                     SNLEIGVIPIDSIFTPIRNVKYAIENYRVEQVTDYEKLVIEILTDGSIPPKEALKEAA
                     KILIQHFMLFSDEKIEVETVEGETTSEFDEEVLHMRQLLKTKLTDLELSVRALNCLKA
                     ADIETLGQLCSFQRNDLLKFRNFGKKSLAELEEKLISLNLSFGMDVSKYKLDKD"
     gene            34805..35335
                     /gene="rplQ"
                     /locus_tag="PLM04_01510"
     CDS             34805..35335
                     /gene="rplQ"
                     /locus_tag="PLM04_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HON01791.1"
                     /translation="MRHNKKFNHLSRTSSHRKALLSNMASSLILHKRISTTLPKAKAL
                     RVYVEPLLTRAKEDTTQSRRLVFSYLQNKEAVTELFQNISVKIANRPGGYTRILRTGF
                     RLGDNAEMCIIELVDYNENMLKDKTTKKTTRTRRSSSRKKAETEVQAAPATSEVSAPK
                     PVEQAPAENQAPTTEE"
     gene            complement(35483..36484)
                     /gene="gap"
                     /locus_tag="PLM04_01515"
     CDS             complement(35483..36484)
                     /gene="gap"
                     /locus_tag="PLM04_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HON01792.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIEVVAVNDPFLDKDYLIYM
                     LKFDSVHGKFQGTAENKDGKLIINGKEVLFFAEKDPANIGWGAAGAEYVVESTGVFTT
                     VEKASAHLKAGAKKVVISAPSNDAPMFVCGVNLDAYTKDMDIVSNASCTTNCLAPLAK
                     VIHDNFGIVEGLMTTIHAATNTQKTVDAPSNKDWRGGRSILNNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMSMRVPTADVSVVDLTVRLEKAATYEEIKAAVKAASENEMKGILGYTE
                     DAVVSTDFIHDPRTSIFDANAGIALNSNFVKLISWYDNEWGYSNKVLDLICHMDSVK"
     gene            complement(36712..36966)
                     /locus_tag="PLM04_01520"
     CDS             complement(36712..36966)
                     /locus_tag="PLM04_01520"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HON01793.1"
                     /translation="MKTNVGSTDKVVRLALAIVLIVLFYKGIFSVILGIVALIVAFVL
                     TLTSLIGFCPLYTLFGINTAKKKEQSENAEQPTKKRQPKN"
     gene            37280..38080
                     /gene="proC"
                     /locus_tag="PLM04_01525"
     CDS             37280..38080
                     /gene="proC"
                     /locus_tag="PLM04_01525"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HON01794.1"
                     /translation="MEIKNLKIAIIGAGNMGGALARGWSKSDILSAENIAISDISQEK
                     LDQLTAFNPHFTVSTSNREIIRQADIVLLAIKPWLVEAVAKEISKYIDYKKQVIISIA
                     AGVSFETLTHFFDSNGVFFRVIPNVAVEIGQGVSVVSSFNACKEHQQLLLEIFNVLGK
                     TFFVSENQMDAFMALTSCGIAYAFRYVRASVAGAVEMGISPQMAQEAVIQTVRGAVDL
                     LELHQSHPETEIDKVTTPGGFTIKGLNEMEANGFSNAVIKGLKASYSK"
     gene            38126..38674
                     /locus_tag="PLM04_01530"
     CDS             38126..38674
                     /locus_tag="PLM04_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HON01795.1"
                     /translation="MNIQIKQIAERLRALRESLELTPEQAAEKCGATTEEYQQYESGD
                     YDIPMSFICQAADSFGVATTALLVGDDPHAQVFFVTRKGTGASVERTKAYKYQALAYG
                     FRHPKAEPFEVTVEPNDNPLTLNTHFNQEFNYVLEGTLLLRIAGNDLILNEGDSIYFD
                     ATRPHGMKALNGKRVKFLAVII"
     gene            38780..40435
                     /locus_tag="PLM04_01535"
     CDS             38780..40435
                     /locus_tag="PLM04_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON01796.1"
                     /translation="MLEKYLKQTEFKDFDDFKANYQLIVPEHFNFAYDVIDEWAAKEP
                     DKLAMIWTNDQGECRKFSFAEMKKYSDQTASYFQSLGIGKGDVVMAILKRRYEFWFTI
                     LALHKLGAITIPDTHLLTPKDLIYRNNAADIKAIVAVGEEPIVSHINDSMAESPTVEM
                     LISIGPIVPENWLDFHKGIDEATPFTPIHHVNENTDPLIISFTSGTTGNPKMVLLDCL
                     YPLGHIITAKYWHNLHENSIHLTIADTGWLKALWGKIYGQWIVGACVFVYDHEKFTPA
                     NMLEMIEKYHVTSLCAPPTIFRFLIREDISKYDLSSLEYATTAGEALNASVYEKLYEL
                     TGIKLHEGYGQSETTVCVYTTPWIEPKPGSMGLPGPHYDVDIQMPDGRSAEVGEQGEI
                     VIKLHKNYPVGLFKGYYRNEELTREAFRGNVYHTGDMAWKDEEGYFWFVGRTDDVIKS
                     SGYRIGPFEVESALMTHPAVVECAITGVPDELRGQVVKATVILAKEYKDKAGEELVKE
                     LQNHVKKVTAPYKYPRIIEFVDELPKTISGKIRRVEIREKDKQ"
     gene            40497..41150
                     /gene="upp"
                     /locus_tag="PLM04_01540"
     CDS             40497..41150
                     /gene="upp"
                     /locus_tag="PLM04_01540"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HON01797.1"
                     /translation="MKIINLSDNNSILNQFIKEIRSVEIQKDSMRFRRNMERIGEIMA
                     YEISKAMDYVEEKVQTPLGIAPVKVIDEKVVISTILRAGLPFHQGFLNYFDGSENAFV
                     SAYRKYRNAENFEIFVEYIASPGLEGKTLILTDPMLATGSSMEQTFSALTTKGFPSKI
                     HIATVIASRHAINYLTTHFPENITTIWAAAIDPEINSHSYIIPGLGDAGDLAFGEKL"
     gene            complement(41207..41461)
                     /gene="rpsT"
                     /locus_tag="PLM04_01545"
     CDS             complement(41207..41461)
                     /gene="rpsT"
                     /locus_tag="PLM04_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HON01798.1"
                     /translation="MANNNSAIKRIRQANKKRLHNRYYIKSTRTAIKRLRTTTDKEKA
                     AELLPQVSSMLDKLAKRNMIHKNKANNLKSKLAIHVNKLA"
     gene            complement(41492..41566)
                     /locus_tag="PLM04_01550"
     tRNA            complement(41492..41566)
                     /locus_tag="PLM04_01550"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41531..41533),aa:Glu,seq:ttc)
     gene            41861..42583
                     /gene="recO"
                     /locus_tag="PLM04_01555"
     CDS             41861..42583
                     /gene="recO"
                     /locus_tag="PLM04_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HON01799.1"
                     /translation="MQVKTEGIVFHSLKYSDNSAIVTIFTRRFGRVAYMIYGRGKRKS
                     SWKPAFFQPLSIVELEVSHDMRKNIQQMKDVRVLVPYQSLTIDPLKRSIAMFIAEVLL
                     KVLSQSEADESLYLFLRQSLEFLDNSKQKTANFHLVFLLKLSRYLGFEPNIDGQTTLF
                     FDLVNGVFLSSPPAHRHYLFAEEAGKMAQLLQTDYEHMTEIKLSRQQREKLLEDIVSY
                     YRFHLPDFPEIHSLEVLKTLFD"
     gene            42782..43351
                     /locus_tag="PLM04_01560"
     CDS             42782..43351
                     /locus_tag="PLM04_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HON01800.1"
                     /translation="MEKSNEITMEKLLAELENLKAKTTQLENSAKKDQLSIAIISGDM
                     DKIMAGMIISLAAAAMDTKVKLFFSFWGLAALRDPKKTVKGKNFVSKMFGCMLPKGYG
                     KLKLSKHNMCGMGPMMMKNLMKKQHVLSLDEMFKEAAELGIEITACEMTMNLMGFKKE
                     EFIDYPHFRIAGAATFVADAGESAMQWMI"
     gene            43390..43641
                     /locus_tag="PLM04_01565"
     CDS             43390..43641
                     /locus_tag="PLM04_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HON01801.1"
                     /translation="MTTEELKNLTVAKSVDARGTACPGPLLEAKKAIGTIQSGEILEV
                     LSADEGTKNDLPKWCNKQGNEFLGMVEENGYYKLYLKKK"
     gene            43653..44066
                     /locus_tag="PLM04_01570"
     CDS             43653..44066
                     /locus_tag="PLM04_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HON01802.1"
                     /translation="MTSFVKKNAKILVFSTEKISDPAIDMAGLLKLHYPPTVYTITVP
                     CSSGIKPNWILHAFEKGFDGVFIAADGTDCVFGESCTEKTGKVVAMTHEKMKQNNLDP
                     ARLRMAALCSVCSDAFVRQVHSLNEYLIKTRYSEK"
     gene            44070..45368
                     /locus_tag="PLM04_01575"
     CDS             44070..45368
                     /locus_tag="PLM04_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HON01803.1"
                     /translation="MEERRKKIEFDALVIGAGIAGGETALNIANNGYKVLLVERDHSI
                     GGKMILLSKVFPTLDCAACITTPKVSEIARHPNITVFTESEVTSIVKKTESLFIAKIT
                     KNPRYVHVEDCTACQLCEKACPVSVRDEYQFGLVGRKAAYIPFSIANPKAAVIDLEQC
                     TLCGACERVCPTHCIDFTMETEYFELQVKTVVLATGFQLFDPLKIPRFGYGKYKNVVT
                     SMQMEREIAPTRPYHTILRPGDGKIPDQIAYVLCTGSRDDSVGNPVCSQVCCMYSMKQ
                     AQLLMGALPMADITIYYIDIRSFGKGFEEFYQQAKDMGVNFVKGKIGKISEKENGNLI
                     LRYEDIQEGIVKEAEHDMVVLSVGMLSNQSVHQIFDGEMLQFDSLNFIWQSDLLGSPA
                     KTNIDGVFVAGTASGPMDIPDSILSAGSASSEAISYLIQH"
     gene            45377..47104
                     /locus_tag="PLM04_01580"
     CDS             45377..47104
                     /locus_tag="PLM04_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON01804.1"
                     /translation="MKKNLGVYICHCGGNISDYVDVEKVRQAIEHEEGVVLAKTTMFA
                     CSDSNQKEMVEDIHQYQLDGVIVASCSPKLHLVTFRNVTETAGLNKYTYVHANIREQA
                     SWAHSDDKEGATEKAIRLVRAAISKSRYAEALYPNKFEAEKAIAVIGAGMAGMKAALS
                     IAEKNMSVVLIEKENTVGGHVAEWGSLFMTNEKGSELVQRIHNDLLQHENISILTGTE
                     VVSSKGSIGNFTLTLQKKHQPVDNLRETMEINVGSVVIATGFDHYIPQRGEFSYLNNE
                     NVISLPDFKKLTDQSTGKTLLYNGKEIHDIAYIYCVGSRQSEGENKYCSRFCCISTIH
                     TAIETKKKFKGIRNFHLHRGIRTYGKQELLYDEALQNGDIFIQFHDDFPPEVSFKNKK
                     TSLKVKDLLFSDKELLLDADLVVLVTGMLPRIDQSIGQLFKLPKGRDNFYNEIHMKLR
                     PVETVIDGITLAGTCQGPKNISESVNSALSAATKAFSSISNGLVETEPIVAEINGNSC
                     VWCGECLKACPFEAIEQKEIDGKIIAVVNKSVCKGCGMCAPVCVPNAIDLIGYSDKEI
                     FSMIDALVQ"
     gene            47109..47390
                     /locus_tag="PLM04_01585"
     CDS             47109..47390
                     /locus_tag="PLM04_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01805.1"
                     /translation="MMEAEIGKTAKYLRDKQGIPEKAKENLKEFVRIKKALIDALKNG
                     EMTIPQLSEKLSMSKEDVTFYLMTLIKYGDVAVGEIDDNDEYFTYKLNK"
     gene            47387..48460
                     /locus_tag="PLM04_01590"
     CDS             47387..48460
                     /locus_tag="PLM04_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HON01806.1"
                     /translation="MMASKINPAFQDKLKKYGAVNFNACYNCGTCTAVCSLSTPEESF
                     PREMVRLSSLGLENDIKSSLKPWECYYCGECTENCPQTANPGELMMSLRRWLTSVYDW
                     TGLSGLLYRSVTFTISALIMVFIAVLVLGNAMSFQLAEMMHYGHLFEMYVVFAVLAII
                     FIPNIARMWNFTILRPKVKANLKIYIKSLPDLFVHMFTQKRARDCDKEDNFRWLEHFI
                     LVLAYLSLLFTTVFLDWFGTKSFFVVVLGYVESFFVFVITADFVSSRIRKAKALNKFS
                     QPSDWLFVIWLMLMGLTAFLVRVFIDTHMIDNNVWMYIVHLTVLAQWAIIIVPFGKWS
                     HFLYRSFGMYFSSIKAASEKRKG"
BASE COUNT        15486 a         8626 c        10098 g        14289 t
ORIGIN      
        1 cgctcgagta tttaaaatct cctttattgt aacccatttt acaattttga actttagttt
       61 tttctgccga cacaaattga ctgaaatcct gtttgtattg ttccaatgtt tttggaacta
      121 ctgtgttttc ttcgtcggtt gaacacgatg ctccaattaa tccgatgaat aaaaagaata
      181 aggaaaattt ctttatattt ttcatgacga taaaattttt gtttgaaaat ttttaatagg
      241 ttggattttg cggaattacc atttcattat tggtgtcgat ttcagcttgt ggaatcggga
      301 gcaacaacct ttccttgata taaaatcttt cttcaggcgg ttgcatggga ttgtattttg
      361 aataaagcac tgcccaatct gtttcaaagg tatgtctcct taaaatctcc atcggtttgt
      421 ccggattgtc atacgattta gccatgcgaa gcaaatcaaa ccagcgctga ttctcaaatg
      481 caagctccaa gcgtctttcg gcatatacag catctaaagc ttcttctttt gaaccaaaag
      541 gaccaattcc atctagtccg gctcttgctc tgacatcatt aagcggattg tgcgcatttg
      601 caaaattccc gtcctgagcc aaaatttcgg cataaagcaa ttttatgtcg gcaaaacgta
      661 aaataatcca gtcattttca ctttgaagcg gttgggtcat agatgcatcc atatatttgg
      721 aaatataagg cactgaaaca gttgacgatt tattccatac tttgaaacaa gcatccttac
      781 gagtatcatt tggatttgca ttgaacaagt tcatcaactc ataagtggga ttattataac
      841 caagcggtgt accgattttt aagaataagt ttgcagaacc caaaggtgca aaagtgcccc
      901 agaatggtga accaatgccg gaactaccgc ctttgtatcg tacggcaaaa attatttcct
      961 tgttcatttc gttgctaata ctgaaaatat ttgcataagc acctgtacca gttaacaaac
     1021 catgaggcga tagtgttaga atgtcttcca attctttttt agccaatgat aaattatcct
     1081 ttccgcccaa catcatgtaa gccttggcca acattccttt ggctgcccac ttggtggctc
     1141 ttcccaagtc tgttgctgag taggtggaag gtaattcttc aactgccttt ttcaaatcgg
     1201 gaataattat ttcattatag atttctgaaa ccggcgaacg aggaattttt tttgcttcat
     1261 ttggcccgat aactttggtt attttaaaca tatcgccaaa caaattgacc agcgtgtaat
     1321 aatgataagc tctcaaaaac aaaagttcac cttcatattg tgctctgagt gattcatttg
     1381 caacataact attgtctctt acagaaggta gcactgcgtt aatattggaa atattctgga
     1441 aaattttata ccagtaatcg cgcaaagcag gcaaacttgg agaaagccta aaagaagcta
     1501 tatcggtttg atcaattctg attgttgcac tcgagcctgt ttcactttgg catgaattgt
     1561 cgctcctgag ttcggtatac atccattcat ctttctgtat atttgcgagg ccagcatagc
     1621 agccgattac tccctgattg attcgtatag gggttgtata gaaatcaacc tgtgtaagta
     1681 ctgactcagg cttgtccgta aggaagtctt cacaacttgt caaaaagaat ccacacagcg
     1741 aaattgctgt aataaatatt ttatttttca tatttgtatt tttatttagt atggtcataa
     1801 taaaaagctc cttaattaaa aattaatatc caatcctgcc gtaaaggttc tgttcaaagg
     1861 gaaagcacca cgttgataac cgtctaccaa tgggctagtg taattgctag ctgttctcct
     1921 tgcttcagga ttaatacctc tataactcgg tgccatgata taaaccaaat tctcggcaga
     1981 aaaatatact cttaaatttc ttaatttcaa tgatttcaat aagttttgag gtaaagtata
     2041 tcctaaagtg aaatcacgaa ttgcgccata agaaccatct tctatgcagt agtcagtcaa
     2101 catcaaatcg ctaccggaag tagtattgga ataaacggtt tttccatctc ctggaaacat
     2161 gggactcaca tatcgattgt tggtgtaagc cttgttgtag cgcagttgtt cattataatt
     2221 tacattgcca ttgataatgt caataccttg aacgccttga atcaaaaaac ttaaatcaaa
     2281 gtttttataa gtaaacgtat tggttattcc ccatgtaaaa tcagggaatg gatctcctaa
     2341 aacagtacgg tcttcggtat caattttatt gtcgccattg atattcacta ctttaattcc
     2401 tcctacaata ggtgcaaatt tggtgtaagt aaacggattg ccgttttcat cttttaaggc
     2461 ctttgctgct gctacttctt caaaagttgt atatacgcca tcggttttat aaccgtaata
     2521 ctgaatggcc ggctctccca ctatggcacg atatacttca ctcctttctc cgaaattatc
     2581 ttcatattcc ttgtctccgt aatttaagag cttatttttg ttggtggaaa aattcgctga
     2641 agttttccat gtaaattttt tattggcgat attggtcgta ttgagctcta tttctattcc
     2701 tttgttgttt accttcccaa tattgttcca ataagattga tgcccggtaa tatacatggc
     2761 aggttgttgt agtaataatt gaatcgtatt ggaattataa tattctatcg aaaagttcag
     2821 tctgctgttc aaaaagccaa aatctattcc gtaattggct tcggatgttt gttcccaagt
     2881 aatatcggga ttccctaacg aagaactgtt agaaaccatt ccttgtacca acgttccatt
     2941 tcctgaaccc agcacatagt tgctggtgtt gatactgttc atgtaagcat attgaggaat
     3001 gttgttattt cctgttaacc cataactgac gcgtaatttt aaattggata gccattcaaa
     3061 attcttcatg aatttctctt cagaagcacg ccatcctgct gaaaatgcgg ggaatgtgcc
     3121 ccatttatta cctttgtaaa actttgagct cccatctgct ctaaaactgg ctgacatcat
     3181 gtatttgcca agataagcat aattaagacg gcctaagaga gaaaccagcg attcggtata
     3241 ataaaaagaa gttgttccat caatagaagg actgtccaat agaatttgag aagcaagatt
     3301 gaaagaaaga atttgttcgt cggggaaatt ggtacctaca atcctgttat aattgtcatt
     3361 ggtttgttgc atggtgaaac ccaataaggc atcaaattcg tgattgctga ttttcttatt
     3421 gtagttcagg gtattttcag aaagtaattc cctatgcgta gtcagttgtc gggtaaggga
     3481 atttggattt cctgccttgt tggcagaggt ttgttctttt ttgttgtatt ctctgaataa
     3541 aagatatgct ccattggaag ttttgaattg taatccgggt aatagattta aagtcaaata
     3601 aatattaccc tgaaatctgt agtcgtcagt aaagatactg gttcgttccc tgatggagac
     3661 aggattctga ttacttgaat tccaaaccgt aactcccggc aagctccaaa cctcattgtt
     3721 gtagcctata ccgctaatat taatgccatt aaagtcgctt ggctgagcat aatctccagc
     3781 aactttaccg gttaaggcag cggtggcttc gttatgcctg attggcagcc aagacgggaa
     3841 tcgcatataa tcagttaaat ccacagcagg tcgttcttgt cggctataag taggtgaaag
     3901 atttactcct aaagtggctg ctttagataa cttggcatcc aatttggctc taaaagtaaa
     3961 tttgtcataa gtactgttca acatgatccc atcttcattt acgtagtttc ccgaaacaaa
     4021 atatttcaaa tttttatcac ctcctgaagc actcagttgg taattctgca tggttccgcg
     4081 atctctcaac ccttcattga gccaatcggt aggttgatca tccaaatatt tcataatcag
     4141 gtagcctgcc ttttcaggtt ctgttatttt attaaatgcc attgtctcgg tggctattcc
     4201 attaacggca ggatccagct ttctcatgtc agcttcacga tacaaaagag ccacatattc
     4261 ttcagtactt aacatgtctg gaagtttcaa tggattccga aaacctgaat acattttgaa
     4321 attgtatttg ggtttgttaa tttctccact ttttgtggtg atcagtatga ctcctcctgc
     4381 ggctcttgaa ccgtacaatg ctgcagagga agcatctttc aacacttcaa cagactgaac
     4441 atctgccatg ctaaccatcg ataagccatc gggaactgga aatccatcta ttactatcaa
     4501 tggatcactg cttgcactga tggaacccaa gccacgaaca cgaatttttg gagctgcacc
     4561 ggcttctgga tttacattat tgatttgaac gccggcaatt ttaccttgta acgcctgatc
     4621 tactctggaa accggaattt catccatttt ctcgtttttt aaagaagaga cagctcctgt
     4681 caagtgagat tttttttgag tgccataagc tacaacaaca acttcgtcaa tggtttgggt
     4741 ttcttcttcc aaaataacag tgagttgtcc actggcaggt attgttcttt ctattgtttt
     4801 gtagccaata taagaaaaaa ccagtactga ttgtctatcg gggacgtcaa tgttgaattt
     4861 gccattgaaa tctgaaattg tccccgtcct ggagcctttt acagtaatat tgacaccaat
     4921 taatggttct tttttagtgt ccaacactgt tccagtaact ttaatttgag aaaatgcact
     4981 gattgtacta ataaataatg ctattaatac aaaaacttct ctctttaaga attttggttt
     5041 ttttgttctt ttcatgtttt tatggtttta tacgaatctg ttttactata aatctttgta
     5101 ttttttattg aaaatgagta aattattatt gttttgattt cattctttat aagacaaaaa
     5161 aattggtcaa ccaattattg gtcgaccaaa aatacggtgt ttttataata aaaaaaaatt
     5221 tttttcaggt tattttacga aatatgttgc aaaacatatt ttcagacttg ttgagaataa
     5281 aaaaagctgc ctttttaaca atttgagagc agctttattc catttaagtt gagtctaagg
     5341 aagtattaac gattcagctt agatatctca agccatactc aaacttttca cttttaagtt
     5401 ttcaagtttc gtgcgagcga gagtcataac gccttgcaca tgttggtaaa gcaaggatga
     5461 ggccaattct ccattctgca ttttcactgc attgatcatt tcggcgtgtt ctttaattac
     5521 tacaaaaggg tcggtattac ataccctttc tttattgtag atgatgagaa tatccggaat
     5581 aatggtcata agcatggatt tgatgaccgg attgtgactg ccttctgcta tgttgcggtg
     5641 aaaagcaaag tcataactga cacgtaaaat ttcatcatta tggaatttga taaaattctg
     5701 atgagatttt tccattgcct caatgtcttc ttcggtacgt ctttcggcac atagccgaat
     5761 ggcattcatt tcgaggatac atcttgtttc aaccaacgaa taaaaatcgt aactctgaag
     5821 atttaaaaca tcagaaagta agccgtttaa tgaatttata tccagcccat tgagaatagt
     5881 tccactctgt ggaagtgttt ttacgatgcc ataaaattcg agtttttgta atgcttcgcg
     5941 aatgtgtgtc cgcccaaaac caaagtcgct tgccaatttc ctttcggcag gcaatcggtc
     6001 tccgggtttt aattcacctg actcaattaa ttctttaatt ttattgatta cttcatctgc
     6061 aggcgatttt tgctgggctt tcatcaatcg gataaaatta tcttccatca taatatttgt
     6121 agttttttct attgacttat ttgttccgaa tatttctctg ccgaaaaaaa ggaaaaattt
     6181 taaaattatt ttttaacccg tttaattggt tgaccaaaat tacacaaatt tttttacctt
     6241 caaaattttt tccaataatt tgttttcttc gttttaaatt tctttttctc aattccaaaa
     6301 aacaacattt ttcaaattcg gtaatcaaaa attgggaaat aaattctttt attagacttt
     6361 ttcatgatgt ggaataaaca aaatcctttt attttcatta attttgtcag acaattatgt
     6421 cattattgca gtaaattatt gttagaaaca tgcaaccatc agaaatacaa gctgtaaaac
     6481 aacgttttgg aattattgga aattctgaat tgttaaatcg ggctatcgat gtggccgttc
     6541 aggtggcgcc aacggattta tcggtattga ttaccggcga aagcggtgtt ggcaaggaaa
     6601 attttcctca aattattcat tattacagtc atcgcaaaca cggaccttat attgccgtca
     6661 attgtggcgc catacctgaa ggaacgattg attcggaact gtttgggcac gaaaaaggtt
     6721 cattcaccgg agctatggcc gaccgaaagg gctattttga agtagcagac ggaggtacta
     6781 tttttttgga tgaagtgggg gagttaccgc tttccactca ggttcgctta ttgcgtgtgc
     6841 tcgaaacagg tgaatttatc aaggttggtt cttcaaaaat tcagaaaacc aatgttcgtg
     6901 ttattgctgc caccaatttg aacatgacgc aggcagttag ggacggacgt tttcgggaag
     6961 atttgtatta tcgtttgaat actgttccta tttatgttcc gcctttgagg gaaaggaaag
     7021 aagatattgt tctgcttttt agaaaatttg cagccgattt tgccgaaaaa taccacatgc
     7081 cacccattaa acttacggaa gatgcaaaaa aactgctcac cgaatattat tggaatggca
     7141 atgttcggca acttaaaaat attaccgaac aaatttctgt cattgagcag caacgggaaa
     7201 ttacagcaga aattttgaaa caatatctgc cgaaggatga attggagcgt tttcctgttt
     7261 tgttgtccaa tcaccgaagc gatcagaaaa ctttcacttc cgagagagaa atcctctatc
     7321 aggtgctttt cgacatgaaa caggatatga tagaactgaa aaaactggtt tacaatttaa
     7381 tgaatgctca gggcaatcag gaattgctac aaaaagactt ccctttatcg ccttcttatg
     7441 ttgctccgca tgagactttt gttccggcta caccggtgcc ggtggacgtg ttgaaacctg
     7501 tcaataattt ggggcgcgaa gtaaatcagt tccccaatac aaacaaacaa aattcttttg
     7561 aagaagctgc tgaattccaa gagattgaag atatacaaga accaatgacg ctcgaagata
     7621 tggaacgcca aatgataaag aagacgctcg aaaagcataa aggcaaaaga aaagcagctg
     7681 ctgaagaatt gaaaatttca gaacgaactc tgtaccgcaa aataaaagaa tatggaattg
     7741 aatagaaaac cgaaaaactc attttttgga agaaacacga aatcggtcaa actattatcg
     7801 gccatttttt gtggagtttt tcttcttttg gcttcctgta ccgtttcata caaattcaat
     7861 ggagcttcta tcgattacac taaaatcaaa actatttcca ttgccgactt tccaaatgtt
     7921 tcggagttgg tttatccgcc tttgtcgcag caattttccg aagcactccg cgatatttac
     7981 accaagcaga cccaacttcg gcttgtccgt caaaatggag atttgaattt ggaaggcgag
     8041 attgtcggct atcagcttac gcctatgtcc atcagtgccg atacctatgc ttccgaaacc
     8101 aaactgacgg taaccgttaa ggttcgtttt accaataagg tgaatccggc agatgatttt
     8161 gaaaaaacct attcggcttt tcaggttttc gacagctcaa aaatgttgaa tgatgtgcag
     8221 gaagagctgg tgaaggcgat tattaccgag attacggaaa atatttataa cgacactgtt
     8281 gctaaatggt aaggaaatgt tttttctggt ttttttgagc atttaggaat aatgacacag
     8341 gcggaatttt ttacactttt gcagcatccc gaaagcatta catcggaaca tactcctgat
     8401 ctaaaagatt tggtggagca ttatccctat ttttcgcagg caaggatgct ttacctgaaa
     8461 gctcttcaag tgtcgggtag tattcatttt gaaaaaacca taaaaactgc agttgtctat
     8521 acttctcaac gtcgctggct ctatcatttt ctttatccgg aagtttttgc aaaggaagtt
     8581 tatcgtcgtc ccgaaaaagg aaaacttaca ggttcttatt tcgacttatt ggatgtagtt
     8641 gaaaaggaag gaggagattc aggtcagact ttgaaaaatt tggccgatca gttgaaaaaa
     8701 gcgcgagcta tggtgatgga aaatccaccg gcaccagatg aagaagaacc cgttaagaag
     8761 aaagagtcta caaaatcgcc cgcactccaa tcggaatatt ttgcggaaga aattattccc
     8821 gagcttgaaa tttcagaaga atcggttaaa aggttaatta agaacagaaa atatactgaa
     8881 gcattggaga ttttaaaaaa attaaatttg aataatccga aaaaaagtgt ttactttgca
     8941 gaccaaattc gtttcttgga aaaagtaata gaaaacttaa aaatttaaat aatatacttt
     9001 tatgtacctt ttgtttacta ttttaatcat aattgcctcc attctcctga tacttattgt
     9061 gttggtacaa aattcaaaag gaggtggact ggcagcgggt ttttcttctt ccaatcaata
     9121 catgggagtt cgcaaaacag ccgattttct ggaaaaagct acatggggat taattgcttt
     9181 cattgtggtg ctttccattg gtgccaccgg cattcacgcc aatcagaaat ccggaggaag
     9241 cgattccgaa atcaaagaac agtatcaaaa agcccaggaa gaattaccgg gcgttgtggc
     9301 tcccgatttt ggtgaaactc aaggagcagg taacaatcag caagacaata atggcggtca
     9361 gcaaaataac ggaaatcagc agaaggctcc tcaaaaatta cctgttgtac ctgcaaaata
     9421 attagaaaaa atagtcaact ttatttttga aagcacgttg aatattcgat gtgctttttt
     9481 atcgatcatg aaacaaaaaa gacagacagg aaacttttcc catctgtctt tcttttttat
     9541 gaatcgtttg attagaaatt atatctcacc cccagcgcaa tgccatccca tgctcctccg
     9601 taactggaag gcagtacgta ccagccggga cgataatcca gtgaaagttg caagggaata
     9661 ttgaaattga attcaatacc gatttgtcca acaattccca atgccagtcc atcatagtct
     9721 ttattgtcct gatttgacca actgccaatt tgcggtccaa ggccaacata ccagttaaaa
     9781 ccgggagaaa gttccgacaa gtcaaaaacc cattgataaa ctcctgccaa attgaatccg
     9841 tgatcgtagt aaaatcccaa atcaccttca attctgttct gattgttcaa gggatgctga
     9901 taggaaactt cccctccgta tcccagtctc acacccagcg cttgacctct gacctgcgca
     9961 tttaatccca taaaagatgc cgaagcaagt aacaaaacaa ttaatttttc tttcatattt
    10021 ttcagttttt tgtttgataa taaatagaaa aaaattgcga agtacagaat taagaaatag
    10081 gttctgtcca gtcgccattt attttaatga gttcgatcaa tttttccacc gcttcttctt
    10141 caggaatatt tttttcaaca caaatctttt tcttgtacaa actaacttta cctcgccctg
    10201 ctccaacata tccgtaatcg gcatcggcca tttctcctat tccattgaca atgcaaccca
    10261 taatgccgat ttttaatcct tttaagtgag aagtttttga ctttattcgg gaaacaacgg
    10321 tttgaaggtc gaacattgtt ctaccgcaac cgggacagga aataaattcg gtttttgaga
    10381 ttcttgccct tgttgcctgc aaaattccaa acgctatgga attaagtttt tcggacggga
    10441 tggttccttc attttttaaa aaaattccat cgccgaatcc ctcaacaaat aacataccaa
    10501 aatcaacccc ggctttaatt tccaagtctt cctccgtgtt ttctttatag ctttgcgtaa
    10561 cgattacggg aactgtgcaa ccggcattca taagtgaatg aaaaaaagct ttttgttcag
    10621 ccggtgcatt tttatgatgg ctttcgagaa taaccactgt atcggccgtg tttttaagaa
    10681 aatgcacatt ttcttttgtc agttcgggga acgacatttt taggaacttg atgcgggagg
    10741 aatgttttat tattttttct ttttcatcga tgtcaaaaat gggaaaaact tcattgtgga
    10801 tgtttattat ctgattttcg gcgaaataat aatcgggagt aatctcaaaa ttttcttttg
    10861 tgtgagctat aaccacagga acattatttc ctccgatatt ttctacggca tgtgttgtcc
    10921 gtcgatgata attgaaagga gaatgttgga agtttttctc ggcaattatt ggttcgtgat
    10981 ttttttttgt gtcaatataa gaaatcagtt ttcgagctac aggaatttct tcttctgggt
    11041 cttcgctcag cgaaaccctt atggtatctc caattccttc ggaaagaagg gtgccaattc
    11101 ccactgctga tttgattcgg ccttcctctt cattgcctgc ttccgtaacg cccaaatgca
    11161 aagggaaatg aaaattttca gcttccatcc tttccaccag cattctaacc gaatgaatca
    11221 tggtaagggt gttggatgct ttcatggaaa tcaccacgtt gtgaaaattt tcttctctgc
    11281 aaatccgaag aaattccatg catgattcta ccatgccttc agccgtatct ccataccgat
    11341 tcataattct ttcgctcaaa gaaccatgat tgacaccaat acgaagcgcc gtttgatgtt
    11401 tattacaaat attcagtaac tgtttaaagc gttttcttat cctttcatat tcagctttat
    11461 attcttcatc attataatcg gaagtaatgt ttcccctgtt gcttcccaga taattaccgg
    11521 gattgatacg gactttttct acatattgag ccaccacatc ggccacattg gcgttaaaat
    11581 gcacatcggc cgacaacgga gtttgatacc ctttatcacg caataaatcg tgaatttttt
    11641 ttaaactttc cgcttctctg tttccttgag tggtaaagcg aaccagttcn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aaatacattt attaaaaata atttaaaaac
    11821 aataattatt atcaatatac gaaaaaaaag gtaatttcgt atatgaattt cgactgaaaa
    11881 atgtattttt gtatagattt ttttgataaa atgacgcatt cttatgatga tttggtcgca
    11941 acttttgaat ccaatctgcg aaagctgatg gaggatttca agtcattgca ggaggaaaat
    12001 tcgttgttaa gagcgcagtt gagcaataaa gaacaagata ttgaactctt aaaagtcaag
    12061 ttttccgagc ttcaaaaaaa ttatgaccat ttgcgaattg ctaaaacttt gtccctttct
    12121 gatgaagatc ggatcaagtc aaagcagcaa atcaacaaac tggtgcggga aattgataag
    12181 tgtttggctc ttttgttaaa tgattagagc ggttatgaac gaaaacgata cttttttaat
    12241 aaatgtacag ataggcggtt acaggttgcc attgagaatt gaacgcaaag aagaagagtt
    12301 gtatcgattg gcagaaaaag ttttgggaaa atatctggaa gtttatcaaa agaaatacag
    12361 tcatcggtcg gcagaagaag tttttatttt tgttgcctac gagcttgcag taatgcttgt
    12421 cagaaaaatg atgcagcagg atactgcgcc tctggcagaa aaagtgtctt ctctgaatca
    12481 ggaactggaa aaattattgg ctgaaaagta attttcgaag aatcgttctt caaacaattt
    12541 ttctcgcatt acgttgttta gttggttaaa aaaattttaa cccgaagttg tcaacgttaa
    12601 tgcgtttttt tattatcaaa ttgaaattgt aattaattgt ttatcaaata aatatatata
    12661 cgaaatgaat ctaattattg ccattataat atcttttggt gttggtgtag gcggtagttt
    12721 catttacttc aaacgcaatc ataaaaaagc tttgaaagaa ctcgaagccg aagcggagtt
    12781 gatcaaaaag aacaacctga ttgaagtaaa ggaaaagttt attgcgctga aagccgaaca
    12841 cgaacaacag gtacagcaaa gaaatgccaa aattcaggca gccgagaccc gaattcaaca
    12901 gcgagaaatg caactcaacc aaaaacaggg agaattgcag cgaaaaatga atgaagtgga
    12961 tgtgatgaaa gagaacttgg aaagtcagtt tttaactgtc gaaaacaaga aaaaggaact
    13021 cgaacatttg atccgtcagg ctcaggaaca attggaaact gtttccggac tttcttccga
    13081 gcaggccaaa gaacggctta tcgaatcgct gaaagaagaa gccaaaacgg aagctatggc
    13141 ttatgtcaac gatattatgg atgaagccaa gatgactgcc aacaaggaag ccaaaagaat
    13201 tgtcattcag agcattcaac ggttggctac agaaacggcc atcgaaaatt ccgttacggt
    13261 gttccatata gagtctgatg aaatcaaagg tcgcattatt gggcgcgagg gaagaaatat
    13321 tcgtgcactt gaagctgcca ccggagttga aatcattgtg gacgatacgc ccgaagccat
    13381 tgtgctgtcg gcttttgatc ccgttcgacg cgagattgca cggctttctc ttcatcagtt
    13441 ggttaccgat ggacgtattc atccggcacg cattgaagaa gtggtcaaca aggtgcgtaa
    13501 gcagctggaa gaagaaatta ttgaagtagg gaaacgtacc accatcgatt tggggattca
    13561 tggacttcat cctgaactga ttcgtttggt gggaaaaatg aaatatcgtt cttcctatgg
    13621 tcaaaatttg ctgcaacatt cacgagaaac agccaatttg tgtgcaacaa tggctgccga
    13681 gttgggcttg aatgtaaaaa aagcgaaaag agccggattg ctgcatgaca taggtaaagt
    13741 tccggacgat gagcctgaat tgccacatgc aatacttggc atgaaacttg ctgaaaaata
    13801 caaagaaaaa cccgatatct gcaacgccat tggagcccat cacgacgaaa tcgaaatgga
    13861 atcgctcata gctcctatcg ttcaggtatg cgatgccata tcgggcgctc gtcccggagc
    13921 tcggcgtgaa atagtagaag cctacattaa gcgcttgaag gatttggaag atttggcact
    13981 ttcttatccg ggcgttctga aaacttatgc cattcaggca ggaagagaat tgcgggtgat
    14041 tgtcggtgcc gataaaatag atgacaagga agcagaattg ctttcattcg atatagcaaa
    14101 acgcatacag gatgaaatga catatcccgg tcaggttaaa attactgtta tcagagaaac
    14161 aagagcagta agctatgcca agtaaggatc tttgaaatta tggggctgca tggatttgac
    14221 aacatgtaga aaaggtatgt aagcatgtcg agcgctgagt ttacggctcg ttaatcccga
    14281 atctcaaaaa attaactggc gaaactaatt acgctctcgc tgcttgatcg aagtatagta
    14341 gattaagctt tatccctgca caaggtgcgg ggacgagaca tcttccaagc gccctctcct
    14401 cgaggcagct tgatacggag gtgcagcaaa atcgaggata gttgcattta gcctcgtaaa
    14461 tgcaatgaaa accatagagg ataaggtaca ggttggtagc ttcggccttg cctgtacacg
    14521 aaaaaccaag cgaagctaag catgtagaaa gcatattgtt tccatgtttg gacgagggtt
    14581 caactccctc cagctccaca aaattcatta attttcaatt actttattag gaatcgcaca
    14641 aatttatgca aattgttgca tttgtttgtg cgttttttat ttgagtaagc tttttgaata
    14701 tttttaaatg gaagtttgct tgtttttcat tttttctcat gttgaaatag tttttttcat
    14761 cattttggta aaagcttttt atcttgcttc attagtctaa cgccatctta acatgcattt
    14821 ttttttgtaa ctttgtgccc caaatttcaa aacaattgga aatgaattta gaacacacca
    14881 ttcaacagct ggttgaacaa gctttacaac aattatatgg gttggaaatc ggtcaaaatc
    14941 ctcttcaagt tcagaaaacg cgaaaggaat ttgaaggcga tatgacggtt gtgctttttc
    15001 cgttcatcaa gttattacgc aagtctcctg aacagatagg gaaagaaatt ggcgataatt
    15061 tgttgaagag ttcatccgtg attgaagctt ataatgtggt gaaaggttat ctgaatctga
    15121 aaattagtgt tccatactgg ctcgaaaggt tgaatgagat atttctacag aaggattttt
    15181 ggaaagttaa gctgaaagaa aatgccgact tgatgatgat cgaatattct tcacccaaca
    15241 ccaacaagcc cttgcatctg ggacatgtac gcaacaattt gctgggatgg agtattgctc
    15301 aaattcagga agccaatgga tggaaagttg ttaaaacaaa cttggtaaac gatcggggca
    15361 ttcacatttg taagtccatg cttgcttgga aattgtttgg caatggcgaa acgcctcaaa
    15421 gttcaggttt aaagggtgat catctggtgg ggaaatatta tgtgttgttc gatcaggctt
    15481 ataagcaaca agtcagggaa ctcgtcgaag aaggatggag cgaagaagaa gccaaaaaat
    15541 cggctccatt gatgcaggaa gctcagaaaa tgcttttggc atgggaaaac aacgatcctg
    15601 aagttcgtaa actttgggaa acaatgaatg gttgggtgta tgaaggtttt aatgaaacct
    15661 atcgtaaatt gggaattgat tttgataaaa tttattacga atccgaaacc tatcttgttg
    15721 ggaaaaaaat agttgaagag ggcttggaca aaggcatttt ttatcgtcgt cccgatggtt
    15781 cggtttgggc cgatttaagg gatgacggtc tggacgaaaa attactgctg agagctgatg
    15841 gtacttcagt gtatatgact caggacattg gcacggcaaa agttcgttat gatgattttc
    15901 ctatcaagaa aatggtttat gtggtaggga atgaacaaaa ttatcatttt caggttcttt
    15961 ccattttgct cgacaagttg ggatttgaat ggggaaaaag tctggtacat ttttcctacg
    16021 gtatggttga attgccggac ggaaaaatga aaagtcgtga aggtacggtg gtagatgcag
    16081 atgatttaat tgatgaaatg cttcgaacag cgcgtgaaac ttccgaagaa ctgggaaaac
    16141 ttgatggttg ttctcccgaa gaagttgaaa atatcgttcg tatggtagca ttgggtgctt
    16201 taaaatattt tatactgaaa gtggatccac gaaagaacat gactttcaat ccaaaagaat
    16261 cgatagattt caatggaaat acagggcctt tcattcaata tacccatgcg cggattcgtt
    16321 cggttttgcg aaaagctcaa gaacagtcaa tttcttatgg aaaattgaat aacttgaacc
    16381 tttccatctc tgaaaaggaa cgttatctcg ttcaacttct tacagaattt cctctaatgg
    16441 tgaaggaagc aggtgatgaa tttaatcctg ccctgatagc caattttctt tatgagttag
    16501 taaaagaata caatcagttt tatcacgatt attccatttt gaaagaagaa aataccgatt
    16561 tgcgcaattt cagattggtt ctttcttcgg ctgttgctga cgtgattaag gatggtatgg
    16621 cattgctggg tatagaagtt ccggaaagaa tgtaacagaa aaaatttttt cacgtgctga
    16681 aataatttct catttctaaa atcatcgttt ttgttataaa caattgaaaa accgattctc
    16741 ttttttgccg caagaaggtt tttttgcgga aattccggac tcactaagcc aaattctttt
    16801 cgggtcgaat gcttttaaga ggcagaaaag caaacagata atgaattttt aaaaaaatat
    16861 tgaggattgc attgtaaata aaaaaataat tcctaacttt gcaacccgtt tgaataagaa
    16921 atcaagaact caaaaatatt tatacaacta ataataaaat gcctacaatt caacaattag
    16981 ttagaaaagg aaggacagaa ctcgttgata aaagtaaatc acctgctttg gattcatgtc
    17041 cacaacgcag aggagtatgt atccgtgttt acactactac gcccaagaaa ccaaattccg
    17101 ctttgcgcaa ggttgctcgt gtgcgtctga caaatcagaa ggaagtaaat gcctatatac
    17161 cgggcgaagg acacaacttg caggaacact ctattgtact ggttcgtgga ggtcgtgtaa
    17221 aagatttgcc gggtgttcgt tatcacattg ttagaggtac gctcgacacg gcgggagtga
    17281 atggtcgtct gcagcgtcgt tcaaaatacg gagccaaacg tcctaaaaaa ggacaacccg
    17341 cagctgcatc aacaggcaag aaaaaataaa attaaacttc agaagtgaag ttttttaaat
    17401 aataaggtta tatttttttg aatatataaa atcaattcaa gtttaaacac ttgtttgagt
    17461 ttttcaggaa gcagatgata gttgagtaaa tgacttaaag aagtcgttga agcatactaa
    17521 gaaagcaact aaaaacaaaa acggaaattt ttaataaaaa atgagaaaat cgaaacctaa
    17581 aagaagggtc gtattacctg atccgatttt caatgatccg atggtaacaa aatttgtcaa
    17641 ccatcttatg ttcgatggca aaaaatccat tgctttcgat attttttatt ctgcactcga
    17701 gaatgtaaaa aacaaactcc ccaacgaaga gaaatcctct cttgaaatct ggaaaaaagc
    17761 acttgaaaat gtaacacctt tggtcgaagt taggtctcgc cgtgttggtg gagctacttt
    17821 ccaggttccc acagaaatcc gtcctgaacg gaaagaatcc atttcgatga aaaatttaat
    17881 tctatatgct cgtaaacgag gagggcattc tatgtccgac aagctggctg ccgaaatcgt
    17941 ggatgctttc aacagtcagg gtggagctta taaacgcaag gaagatattc atcgtatggc
    18001 ggaagctaat cgtgcgtttg cacactttaa attttaattg aaggaatttt tttaaaatgg
    18061 cgaaagtaga tttaagttat acaagaaata ttggcatcat ggctcacatc gatgccggga
    18121 aaaccactac ctccgaacga atcttgtttt acaccggttt aacacataaa atcggagaag
    18181 ttcatgatgg cgctgccact atggactgga tggaacagga acaggaacga ggcattacca
    18241 ttacatccgc tgcaactact acttattgga attacaatca gcatcaatat aaaataaatt
    18301 tgattgacac tccggggcac gttgacttca ctgttgaagt agaacgttcg cttcgaattc
    18361 tcgacggagc cgttgcaact ttttgtgccg ttggtggtgt tgaaccacaa tcggaaacag
    18421 tttggcggca agccgacaaa tataatgttc ctcgtttatg ttttgtcaat aaaatggatc
    18481 gctcgggagc cgatttcttt gaagtggttc gccaaatcag agaagtcttg ggtgctaaac
    18541 cctgcccaat tcaaatcccc ataggcgctg aagagaaatt caaaggtgtg gtggacttga
    18601 taaaaatgaa agccattttc tggcacgacg aaacaatggg agctgaatat tcggaagaag
    18661 aaattccagc cgatttggtg aatgatgccc gcgaatggcg agaaaaaatg ctggaaattg
    18721 tagctgaata tgatgatgca gtgatggaga aattctttga tgatccttct aaaataactg
    18781 aagatgaaat taaggcagct attcgtaaag gaacattagc aaaggaaatt aatccggtta
    18841 tttgcggttc tgcatttaaa aataagggaa tacaaaccat gcttgatgca gtttgtgctt
    18901 accttccgag tccactcgac aatccagaaa ttgtgggaaa agatccgcgt tacgaagata
    18961 aaacggtggt tcgtcatccc gaccccaatg aacctctgtg tgcattggct ttcaaaattg
    19021 ccaccgaccc ttatgtggga cgtttgtgct atttccgtgt ttattctgga aaacttgagt
    19081 cgggttctta cgtatacaat ccgcgaacag ataaaaagga aagaatttcc cgtattttcc
    19141 aaatgcattc caacaaacag aatcctgtag atatgatttc cgcaggagat attggagccg
    19201 gtgtcggatt taaggatatt aaaacaggag ataccctttg cgatgaaaac catcccattg
    19261 tgctggaatc gatggatttc cccgaaccag ttatcggaat cagcattgaa ccgaaaacac
    19321 aaaaagatct cgataagctt ggcgtaggat tgtcaaaatt ggcagaagaa gatccaacgt
    19381 tcactgtaca taccgatgag caatcaggtc agaccattat tagtggaatg ggtgaacttc
    19441 atttggaaat tattatcgac cgattgcgac gtgagtttaa ggttgaatgc aatcagggaa
    19501 ggccacaggt tgcctacaag gaagccatta cacagcctgt agaaatacgc gaagtgtata
    19561 aaaagcagac cggtggacgc ggtaaatttg ccgatattat tgttcgcgtg gagcctgccg
    19621 ataacgattt tgaaggaagt ttgcaattta tagatatggt aaagggcgga aatattccca
    19681 aagaatatat tccttccatc cagaaaggtt tccagtcggc tatgaaaaat ggagttttag
    19741 caggattccc tgtcgaccgc ttaaaggtta cggtattgga tggctcgttc catcctgtcg
    19801 attctgatca actttcgttt gaaatttgtg ctcagattgc atttaagaat gcctgcacga
    19861 aagccaaacc ggttttattg gaacctatca tgaaaattga agtggttact ccagaagaaa
    19921 gcatgggcga cgtaattgcc gacttgaata agcggagagg acaggttgaa ggcatggaat
    19981 cgagccgaac cggagcacgt attataaagg caaaagttcc tttggcagaa atgttcggtt
    20041 atgttaccgc acttcgaacc ataacatccg gtcgtgcaac ttcttcaatg gaattttcac
    20101 attatgcgga agtctccaat tcgttagcaa gggaagtagt ggctgaagca aaaggaaaag
    20161 ttgaattatt acattattaa tgaatacatt tttaattagt aaatgactct taattaaaaa
    20221 ttaaacacac taaaacaaaa tcatcagcga tgagtcaaaa aattagaatt aaattgaaat
    20281 catacgatta caatctggtt gacaagtcag ccgaaaaaat cgtaaaaacg gtgaaggcaa
    20341 caggtgcagt agtaagtggt ccaattccgc ttcccaccca taaaagaatt tttacggtaa
    20401 atcgttccac ctttgtcaac aaaaagtcgc gcgaacagtt tgaattgtca tcctacaaaa
    20461 ggttgatcga tatttacagt tccaccagca aaacggtaga tgcactgatg aagttggaat
    20521 tacccagcgg agttgaagtt gaaattaaag tctgacaaaa tttgagggtt ataaacctca
    20581 aagatcagga aatttgaaaa atatttaatt ataaaataag ctatcaactt tctgtctctc
    20641 gtttacgaaa gacagaaaaa aacaaacaat tgaaatgcca ggattaatag ggaaaaaaat
    20701 cggaatgaca tccgttttca gtgctgatgg aaaaaatgtt ccatgcactg ttatcgaagc
    20761 tggtccttgt gttgttactc aaattaaaag cattgaaacc gacggttatg aagctgttca
    20821 ggtaggtttt gaagaaaaga aaccaaaaca taccaacaag ccggaagcag gacattttaa
    20881 agttgcagga gtagcgccac aaagacactt ggttgagttc aagggatttg aaggagattt
    20941 taaattgggt gatgttatca cagttgaaat ctttgacgga agtacttttg tcgatgtagt
    21001 aggtacttca aaaggaaaag ggtatcaagg cgttgtaaaa agacataaat tttcgggagt
    21061 tggagagaca actcacggtc agcacaaccg acagagagcg cccggttctt taggagcttc
    21121 ttcttttccg tcgcgtgtat ttaaaggtat gagaatgggt ggacgcatgg gaggcgataa
    21181 agtaaccgtt caaaatttgc aggtattaaa aattattccg gaacataatt tattgctggt
    21241 caaaggttct gttcccggag ctaaaggttc aatcgtaatc atcaataaat aatcatggaa
    21301 ttaagtgtat taaatatcaa aggagaagac accggcagaa aagtgtcgtt gaatgaggct
    21361 attttcggca ttgaaccgaa cgaccatgct atttatttgg atgtaaaaca atttttggcc
    21421 aaccgacgtc agggaacgca taaagctaaa gcccgcagcg aaatttcggg cagcacccgt
    21481 aaattgggaa gacaaaaagg cggaggtggt gctcgtccgg gcgatattaa atctcctctt
    21541 agagttggtg gcggacgtgt ttttggcccg gttccacgcg attacagctt caagttgaat
    21601 aaaaaagtta aacagttggc tcgcaaatct gctctttctt ataaagccaa aaatggagca
    21661 attaccgtag ttgaaaattt tgaatttgaa gctccgaaaa caaaaaaaat gctcgaattg
    21721 gctaaaaatt tacaagttgc tgataaaaag cctttaattg ttttatcaag tgcaaataaa
    21781 aacgtatatt tgtcggctcg taatttacct tcaatagatg taataacgac ttctgaacta
    21841 aacacttaca atgtactcaa tgcaaaaact cttgtattga cggaaggatc ggttactatt
    21901 ttggagcaaa tttttaaagt agaataagga ggaatataga aatgggagtc atattaaaac
    21961 cattggtaac agaaaaattg acaaagcttg gcgacaagtt taatcgctat ggctttaggg
    22021 tacagaagga tgccaataaa ctgcaaatta agcaggctgt tgaaaccatg tacaacgtaa
    22081 cggtagttga tgtcaacaca atgattgttc cttccaaaaa acggagccgc tatacaaaga
    22141 gtggagtaat atcagggaaa agaccggctt ataaaaaagc tattgttact gtaaaaaaag
    22201 gagaagaaat cgattttttt agcaatattt aaaaaactaa aatggctgta cgtaaattat
    22261 tgcccacaac accggggcag agaaataaaa ttattagcaa gtttgacact attacgaaaa
    22321 gtgaaccgga aaaatccctg gtttacggaa aacgtaagac cggtggaaga aataataatg
    22381 gtaagatgac catgcgctat attggcggtg gtcataaaaa gaaattcaga gttatcgatt
    22441 ttaccagaaa aaaagatgga atcccggcta cagtgaaggc tattgaatat gatcctaacc
    22501 gttcggcacg catcgcattg ctttattatg cagatggaga aaaatcatac attttagctc
    22561 ccaacggttt gcaggtaggt cagaaaatta tttcgggcag cgatgtagca cctgaagtgg
    22621 gtaatgcact tcctttggaa aacattccac tcggaacgat agttcacaat atagaacttc
    22681 gtcccggtca gggagccgca atagcccgtt cagcaggtac atttgctcag ttggtttccc
    22741 gtgaagataa atatgtcatt ataaaattgc cttcgggaga agtcagaaaa attcttggtg
    22801 catgcaaagc cactgtagga agtgtaggaa attcagacca tgcgctcgaa aaatcgggta
    22861 aggcaggtcg ctcccgttgg ttgggtagac gtcctcgcgt gagaggtgtt gtaatgaatc
    22921 ctgtagatca cccaatgggt ggtggagaag gtcgtgcttc cggtggtcat cctcgttcgc
    22981 gcaaaggact tttggctaaa gggtacaaaa cacgtcatcc caaaaagcag tccagcaagt
    23041 acataattga aagaaggaaa aaataattca ataatgaagt aaatcaatat gagccgttca
    23101 ttaaaaaaag gaccttatat cagtctgaaa cttgaaaaga aaatacttga catgaatgaa
    23161 agcggaaaga aaaccgttgt caagacatgg gcacgtgctt ccatgatttc gcctgatttt
    23221 gtgggtcata ctattgctgt tcacaatgga aacaaattta ttccggtgta tatcaccgaa
    23281 aacatggttg gacataaact gggtgaattt gcactgacgc gtactttccg tggacatgca
    23341 ggaaagaaaa aataaaccat tgaagcagta agttagaaaa ttaaaaaaat tggtaaaaat
    23401 gggtgctaga aaaaaaatat ctgctgatag aagaaaagaa gctcgtcaaa cgctctatta
    23461 cgctaagttg aatgacgttc ctacttctcc gcgcaaaatg aggcttgttg ccgacatgat
    23521 cagaggaatg gaagtaaata aggctttaag cgtgttgaaa ttttcatcaa aggaagcttc
    23581 tcgcagaatg gagaaattgc ttaaatcggc catcagcaat tgggaaatca agaccgaaca
    23641 aaaagccgat aatgctaatt tgtttgtaag caaagtatat gtgaatgaag gaacgacgtt
    23701 aaaaagatta cgtacggccc cgcaaggacg cggttatcgt attcgcaaac gttccaatca
    23761 cgttacttta tatgtggatt caagaaataa taacgataca aatcaaattt aaatagctca
    23821 atgggacaaa aaactaatcc aataagcaat cgcttgggaa ttatacgtgg ttgggattca
    23881 aattggtacg gtgggaaaaa atatggcgac accttgctcg aagacagtaa gataagaaaa
    23941 tatttaaatg tccgcctatc caaagcaagt gtatcccgaa tcattatcga acgaaccctt
    24001 aagcttgtaa ccataacagt ttgcacggct cgtcccggta tcattatagg taaaggagga
    24061 caggaagtag ataagttaaa ggaagaattg aagaaaatta ccgataagga aattcaaatc
    24121 aatattttcg aaatcaagcg cccagaactt gatgcagtga tagttgccag taacattgct
    24181 catcagctgg aaggaaaaat tgcttaccga agagccgtaa aaatggctat tgcttctacc
    24241 atgcgaatgg gagcagaagg aatcaaagtt caggtttccg gtcgtttgaa cggtgctgaa
    24301 atggcacgtt cggaaatgta caaggaaggc agaacaccgt tgcatacttt cagagccgat
    24361 attgattatg cttgcgccga agctttgaca aaagtaggtt tgataggcat aaaagtgtgg
    24421 atttgcagag gcgaagttta cggtaagcgc gatttggctc cttcctatgt tacaggacaa
    24481 aaagaaggct cacgtaccga acgtcgttcg ggagaccacc actcgggagg cgatcatgga
    24541 aataaaaatt cttccagaag aaagaaaaaa taaaaatata atttgatata aggcatcatg
    24601 ttacaaccta aaaaaacaaa atacagaaga cagcagaaag ggcgcatgaa aggaaatgcg
    24661 cagcgcggaa atcaactggc tttcggatca tttggtatca aatctatgga tacagtttgg
    24721 ttgaccggac aacaaataga agctgcacgt atagctgtaa ctcgttatat gcaacgtcaa
    24781 ggtcaggttt ggattcgtgt ttttcccgat aaacctataa ccaaaaaacc tgcagaagta
    24841 cgtatgggaa aaggtaaagg ttctcccgaa ggttttgttg ctccaataac gcccggcagg
    24901 ataattatag aagtggatgg agtaccctat gccgttgcaa aagaagcatt acgtttaggt
    24961 gctcaaaaat tgcccattat aacaaaattt gtagtaagaa gagattatga tctcacagat
    25021 caaaatttgt aaaaaatgaa aacgcgagaa atcagagaac ttagcaccaa agaactattg
    25081 gaacgattag aaactgaaaa agaaaattta gttcgtttaa aactaaatca ttcgatcagt
    25141 acattggaca atccaatgca aataaaagtt gttcgtagaa atattgcacg tattgctaca
    25201 gaattgcgtc tgcgagaaat taatcaataa attaagtaca cccgatggaa acaagaaatt
    25261 taagaaaaga aagaattggt gtagttacca gcaacaaaat ggataaaacc ataactgtag
    25321 ctgtaaggtg gaaagaaaaa caccctattt acggtaaatt tgtaaataaa accaaaaagt
    25381 attatgctca cgacgaaaaa aacgaatgca atataggtga taccgtcagc attatggaaa
    25441 ctcgtccgct gagtaagtta aaaagatgga gattgactga aataatagaa agagccaaat
    25501 aattatgata cagcaagaaa caagacttct cgttgccgac aatagcggag ccaaagaggc
    25561 tttgtgcatc cgagttttag gcggaaccgg caagcgatat gcaacagtag gggataccat
    25621 tgtcgtttca ataaaaagtg taataccttc atcagacatt aaaaaaggaa tggtatcaaa
    25681 agcagtagta gttcgaacaa aaaaagaagt tcgacgtgtt gatggttcat atatacgctt
    25741 tgatgacaat gcgtgtgtat tgttaaacaa tgctggtgaa cttcgtggga gtcgtatttt
    25801 tggaccggta gctcgtgagt tgcgtgccac caatatgaaa atcatttctt tggcaccgga
    25861 agtgctttaa cataagaaaa atttatatag agatgacgaa gttacatatt aaaaaaggcg
    25921 atatagttta cgtaaatacg ggagtagaca aaggtaaaac cggacgcgta ttggaagtgc
    25981 ttgttaagga acaacgtgct attgtcgaag gcattaattt ggtgtcaaaa aacatgaaac
    26041 ccaatgccaa atatccacaa ggtggtattg ttaaacaaga agcgcccatt catatttcca
    26101 atttaaatcc ggtagaaaat ggtcgaccgg tgcgtgttgg tcgaaaactg aataatgaag
    26161 gtaaattagt tcgaatttca aaaaagacag gagaggaaat ttaagaatga atacagcaag
    26221 tttaaaacag gaatacaaag aacgaattgt tccggcttta atgaaggaat ttgcttacaa
    26281 atcggtgatg caggttccca ggttggaaaa aattgttctt aatcaagggt tgggtattgc
    26341 cgttgccgac aggaagatta ttgataccgc aatcaatgag atgacgacca ttactggaca
    26401 aaaagccgta gcaaccgttt ccaaaaaaga tatttcaaat ttcaaggttc gaaaaaaaat
    26461 gcccattggc gttcgagtaa ccttgcgtgg ggacagaatg tatgagtttt tggaacgtct
    26521 gattcgagtt gcccttcccc gtattcgtga ctttaaggga atagaaaaca aactcgatgg
    26581 tcgtggaaat tacacattgg gaattacgga gcaaattatc ttccctgaaa tcaatatcga
    26641 ttccattagt cgattactgg gaatgaacat aacttttgtt actaccgcaa aaactgatga
    26701 agaaggattt gctcttttaa aagagtttgg tttaccattc aaaaaaaatt aaaaaaggaa
    26761 taaaaatggc aaaagaatca atgaaagccc gcgaggtaaa gagaaacaaa atggtggcaa
    26821 aatacgaggc caagcgaagt ctgttgcttg ccgaaggaaa ttatgaagcg cttcaatcca
    26881 tacccaagaa tgcttctccc gtacgtttgc acaacagatg tcagcttacc ggacgtccaa
    26941 aaggatatat ccgtcagttt ggtgtatctc gtattgtatt tcgtgaaatg gcttcaaaag
    27001 gacttattcc cggcgtaaag aaggcgagct ggtaagtttc ccttcaaaaa tatgctggat
    27061 tagtgatttt tcggtttata aaaattacat ttagaatttt accatatatt aaatattgtc
    27121 tcggcagtcg agattaattt aaagtttaaa catttatgac agatccaata gcagattatt
    27181 taactcggtt gcgaaatgca attaaagcaa accatcgggt tgtagaaatt cctgcttcaa
    27241 atttaaagcg tgaaattacg cgtattttat ttgaacaagg ctatatcctg aattacaagg
    27301 ttgttgaaga tggtccacag ggaacaatca aaattgcgtt gaaatatgac cctgttaaca
    27361 aagtaaattc aataaagaaa ttaatcagag tatctaaacc cggtcttcgg aaatatgttg
    27421 gataccagga tatacctcgc gtgttaaatg gattgggtat agctatcctt tcaacatcca
    27481 gtggcgttat gaccaataag gaagcagcca aattgaaaat tggcggcgaa gttttatgtt
    27541 atgtttatta agaggcggag gaaaatgata tgtcaagaat aggaaaatta cccataaaaa
    27601 ttccggctgg agtcaacgtt acgataaaaa acaatttggt ttcagtaagc gggcctaagg
    27661 gaactttaag tcagcaaatt catccaaata tttcagttga agtaaaagat ggtttttgta
    27721 ctttaacccg catcgatgat gagaaagaaa atcgtgccat gcacgggctg tatcgttctt
    27781 tgatcaacaa tatggttgaa ggagtgtcta atggctttaa aaagactatg gagttggtag
    27841 gtgtaggcta tcgtgtttct gctcagggtc aggttttgga atttgcttta ggatattcgc
    27901 acagtatttt tttaaaagta ccgcccgaag ttaaggtaga aacaaagtcg gaacgtaatc
    27961 agaatccgct gattattttg gaaagttgcg ataagcagct tttgggacag gtttgtgcaa
    28021 aaatacgttc tttccgtaaa cctgaaccgt ataaaggaaa aggaattaaa ttccagggtg
    28081 aagtactgcg aagaaaagca ggaaaatcag ccggtaaata atatacgtaa atttgtgaag
    28141 ttatgacaaa attagacaga agacaaaaaa taaaggctca tatacggaaa aaaatttttg
    28201 gaactgctca gcgaccccga atgaccgtat ttagaagcaa cgctcaaatt tatgttcagc
    28261 taattgatga tgaaaaggga ataacattgg cttcagcttc gtcattagct attaaggaaa
    28321 aaataaccaa gcaagagcaa gccggtaagg tgggtgctct aattgctcag gctgctaagg
    28381 aagccggtat cactgaagta gtgttcgacc gcaatggtta tttatatcac ggtagagtta
    28441 aacaattggc cgatgctgct cgtgaaggcg gtcttaaatt ttaagaatgt atggcaaaaa
    28501 tgatgaatag agtaaaaaca acaacggatt tagaattaaa agacagatta gtagctgtca
    28561 atcgggtaac caaagttacc aaaggaggac gaacttttag cttctcggct attgtggtgg
    28621 taggaaatga agatgggatc gtaggctggg gacttggaaa agcaagcgaa gttaccgcag
    28681 caattgctaa aggaacagaa gtagccaaga aaaatctaat caaagtgccg gtgctgaatg
    28741 ggactattcc tcacgaacag gaagcaaaat ttggtggtgc gcgcgttttt atcaaaccag
    28801 cttcttcagg taccggagta actgcaggcg gtgcaatgcg tgcggtgctc gaaagtgtag
    28861 gtattacgga tgtgttagca aaatcaaagg gttcatccaa tcctcacaac ctggtgaaag
    28921 ccacgattgc tgccctctcc gaactccgcg atgcatacac tgtcgctcag aacagaaagg
    28981 tttctttgga taaggttttt aatggataat ttcagataat catggcaact attaaaatta
    29041 aacaagttaa aagtaaaata aaatctccta aagttcaaaa attgacttta gaagcacttg
    29101 gattacataa aatcaatgat gttgttgaac atgaagctac tccacaaatt ttgggaatgg
    29161 ttgagaaagt taaacatttg gtaaagatag tcgattaaaa aattttaatc tttaataaaa
    29221 aggagatatg gatttacata atttaacccc agctgcaggc tcggtaaaat caaataaaag
    29281 aaaaggtaga ggttctggat ctggaatggg gggcacatct acacgtggac ataaaggggc
    29341 aaaatctcgc tcggggtatg cgcacaaaat cggttttgaa ggtgggcaga tgccaattca
    29401 aagacgctta cccaagtttg gttttaagaa tataaataaa attgaatata aagcaatcaa
    29461 tatcgatact ttagaaattt tagcacaaga aaatcaatta tctaaaattg ggatcgatga
    29521 tcttcgtcaa gccggattta tatctaaaaa tcagttggta aaaatcttag gacgcgggaa
    29581 attgaccgta aaattagatg ttgaagctca cgctttttca aaatctgccg aaaatgcaat
    29641 tgttggagcc ggtggaacgg cagtaaaaat ttcataagat gaataaactc attgagacac
    29701 ttaaaaatat ttggaagata gaggacttgc gcaacaggtt attgacaaca ttattgtttg
    29761 tcatgattta tcgctttggt tcttatgttg tgcttccggg tattgatgca ggagcattaa
    29821 ctgctcttaa agctcaaaca agcggaggac tacttgcttt attggatatg ttttcaggtg
    29881 gagcgtttgc caatgcatcc atttttgcgt tgggaattat gccgtatatt tcggcttcta
    29941 tcgttattca gttgctgact atagcagtac catattttca aaaaatgcaa cgtgaaggtg
    30001 aaagtggcag aagaaaaatg aatcagataa cgcgttatct tacagtagga attcttctct
    30061 tgcaaggacc ttcttattta attaatttaa gtgttcagtt aaggcaggct ggttccgcac
    30121 tgccatcagg ttttttcttt caggcaactt ctaccattat tctctgtgca ggtagtatgt
    30181 ttgttatgtg gcttggtgaa cgaattacag acagaggaat tggaaacgga atatcgttta
    30241 ttattttagt cggtattatt gcccgtctgc cgcaagctat tgtaagggaa tttgcctctc
    30301 gattgaccga tgtgggcgga ggtctgatca tgtttattat agaaattctt ttccttttac
    30361 tggttatcgg agcttctata atgctggttc agggaacacg taaagttcct gttcaatatg
    30421 caaaacgtgt ggtaggaaat aaacagtatg gtggcgtaag gcagtatatc ccgctgaaaa
    30481 ttaatgcagc cggagttatg ccgataattt ttgctcaagc tatcatgttt attcccatta
    30541 caattgttgg attttcaaaa tccgagtcaa tgcagggatt tgttggtgct tttatggata
    30601 atacaggttt ctggtacaac tttgtttttg ccatcctgat tattttgttt acgtatttct
    30661 atacggctat cactattaat ccaacgcaga tggcagaaga gatgaaacgt aataatggat
    30721 ttattccagg tgtaaaacct gggaaaaaga cggctgaata tcttgatatg attatgtcca
    30781 gaattacatt gcccggttca atatttctgg ctatcattgc cattatgcct gcgtttgcca
    30841 gtattttcgg tgtaaaccat gcctttgcac aattttttgg aggcacctcc ctgttaattt
    30901 tagttggtgt tacgttggat actttgcaac aaattgaaag tcatttgttg atgcgacatt
    30961 acgacggatt attgaagtca ggacgtatca aaggacgaac aagtggagtt tctgcttatt
    31021 aaataagata acattaaaaa tgatttatct caaatcggaa gaagagatag aattacttag
    31081 aatcagtaat caattggtag ctaaaacgtt agctgaactc gcaaaacata ttacacccgg
    31141 aatcagtacg ttggaacttg atcgaatagc cgatgaattt attcgtgatc atggtgcagt
    31201 gccgagtttt cttgattata acggatatcc taattccatc tgtacttcgg taaacgaaca
    31261 gatagtacat ggaattcctt ctgataaagt ttttttaaag gaaggcgata taatttcgat
    31321 tgactgtggt gcttatatca acggttttca tggcgattct gcttatacct tctgtgttgg
    31381 agaagtagat caacagattg tcaatctgct gaaaacaacc aaagaatcac tttataaagg
    31441 tattgagcag gctcaggaag gacatcgtac tggtgatatt ggttatgctg tacagagtta
    31501 ttgtgaacaa cgcggctatt ccgtagtgcg tgaaatggtt ggtcatggtc tgggtagaga
    31561 gatacacgaa gcacctgagg taccgaatta tggaagaaaa ggcaacggcg taatgctgaa
    31621 atacggaatg gtgatttgca tagagcccat gattaatatg ggaacacgac atattgtttt
    31681 tgaaaaagat ggatggacta cccgaacgct ggacagaaag ccttcggcac attttgaaca
    31741 cgccgtagcc attcgtcagg ggaaagcaga tatactatca agttttgaat acattgaaca
    31801 agttttagga gaaagagcaa tttaattttt atggccaaac aaacagcaat agaacaggat
    31861 ggtacgataa tagaagcatt atcgaatgcc atgtttcgtg ttgaacttga aaacggtcac
    31921 attattacag cccatatttc cggcaaaatg aggatgaatt acataaaaat tttaccggga
    31981 gataaggtga aagttgaaat atcgccttat gatttgacca aaggaagaat tacatttaga
    32041 tacaaataaa aaaatactat cagtgatgaa agtgagagca tccataaaaa aacgttctga
    32101 agattgtaaa atcgttcgcc gaaaaggtcg tctttacgtg attaataaaa agaatccccg
    32161 attcaaacaa cgtcagggtt aattaattta catgaaaaaa attttaatat ggcaataaga
    32221 attgttggag tagacttacc tcaaaacaag cgaggtgaaa ttgcgttgac ttatattttc
    32281 ggaattggtc gcagcagcgc aaataaaatt ttaagtaagg ccggtgttga cagagacata
    32341 aaagtgaaag actggacaga tgaacaagcc gcaaaaatca gggagataat ttcttccgaa
    32401 tacaaggtag aaggagattt gcgctcagaa gttcagttaa acattaaacg attgatggat
    32461 ataggttgct accgcggcat ccgtcatcgt ctcggtttac ctgttagagg tcaaagcacc
    32521 aaaaacaatg cccgcactcg aaaagggaag aagaaaactg ttgcaaacaa gaaaaaagca
    32581 accaaataac agataactgg aaattataat catcagaaaa agaagatata aataattatg
    32641 gcaaagaaaa cagttgcagc caaaaagaga gtagtgaaag tggatgccgc aggtcaattg
    32701 cacgttcatt catctttcaa caacattatt atttcactta ccaacgaaaa tggtgaagtt
    32761 atttcttggt cctctgccgg aaaaatggga ttcagaggtt caaagaaaaa cactccctac
    32821 gctgcgcaaa tggcagctca ggattgtgca aaaatagctt tcgacttggg tttacgtaag
    32881 gtaaaagcct atgtcaaagg gccgggaaat ggacgcgaat cagctatccg aaccgttaac
    32941 ggaataggta tcgaaataac tgagattgtt gatgttacac ctcttccaca taatggttgt
    33001 cgtccaccca aaaggagaag agtataatat caattttcca ctttttgtat gaatcaaaaa
    33061 gggtaatgcc aacaggtaaa aatttaaaaa attcattgca agttttttcc tctctgcaat
    33121 ggcggctgaa gcagttttta caatgtggat acccgaataa attaattata acaatcaatt
    33181 ttagaaaaaa tggcaagata tattggacct aaaacaaaaa tagcacgtaa gtttggcgaa
    33241 gctatttttg gacccgataa agtattgagc aaaaagaact atcctcccgg acagcacggg
    33301 aacaaccgaa gaaagaaaac ttctgaatac ggtattcagc tgcgcgaaaa acaaaaggct
    33361 aaatatacct acggagttct tgaaaagcag tttcatatca tttatgaacg tgcacgtcgt
    33421 gcttccggcg taacaggcgt aattttgttg caaatgctgg aatcgcgtct tgataatttg
    33481 gttttcagaa tgggaattgc acctacacgt gctgctgccc gtcagttggt aaaccacaaa
    33541 cacatcacag tggatggtaa ggtagttaac attccttcct atcaggttaa acctggtcag
    33601 gtggtgggtg tacgcgaaaa agacaaatca atggaagtga ttcagaattc gttgagcggt
    33661 ttcaaccaca gcaaatatcc gtggattgaa tggaatgctt caacgctttc aggaacttat
    33721 ctgcatatcc ccgaaagaga ggatatccct gaaaatatca aagaacattt aatcgttgag
    33781 ttatactcta aataatttgt aaattcatgt ctatattagc atttcaaaaa cccgaaaagg
    33841 ttattatgct ggaggcagac gattttcatg gaaaatttga atttcgccct ctggaacccg
    33901 gctatggcat tactatcggc aatgcattgc gccgtattct cttgtcttct ttggaaggtt
    33961 ttgccatcac ttctatcaaa atcgaaggaa tagatcatga atattctacc attccgggag
    34021 tgattgatga tgtaaccaat atcattctca acctgaaaaa agtgcgtttc aaacaagtgg
    34081 tagaaggtgt tgataacgaa aaagtttcga ttaccgtttc ggatacagca gtttttaaag
    34141 ccggagatat tggtaaacat cttaccggtt ttgaagttct caatccggag cttattattt
    34201 gcgaacttga tccgaaagga agttttcaga ttgacattgc catcaacaag ggacgtggat
    34261 atgtaccggc tgaagaaaac agaacttcca atctcgaaat tggcgttatt cccatcgatt
    34321 caatttttac acctatccgt aacgtgaaat atgccatcga aaattatcgt gtggaacagg
    34381 ttacagatta cgaaaaatta gtaattgaaa ttttaaccga tggttctatt cctcctaagg
    34441 aagcattgaa agaggcagca aaaattctga ttcagcattt catgttgttc tctgatgaga
    34501 aaatagaagt tgaaacagta gaaggtgaaa caacaagcga atttgacgaa gaggttcttc
    34561 acatgcgtca gttgttaaaa accaaactta ccgatttgga actttcagtc agagcattga
    34621 attgtttgaa agctgccgat attgaaacat tgggacaact ttgcagtttt caacgcaacg
    34681 acctgttgaa gttccgcaat ttcggcaaaa aatcgctcgc cgaactggaa gaaaaattga
    34741 ttagtttaaa tctttccttc gggatggatg tttcaaagta taaattagat aaagattaaa
    34801 tgaaatgaga cataataaga aattcaacca tttaagtcgt acgtcttctc acaggaaagc
    34861 tttattgtcc aatatggctt catcgttgat tttgcacaaa agaatctcca ctacacttcc
    34921 taaagctaaa gctttaagag tttacgttga acctcttttg accagagcca aggaagatac
    34981 gactcaatca agacgtttgg ttttcagtta tttgcagaac aaggaagctg taaccgaatt
    35041 gtttcaaaat atttcggtga aaattgcaaa tcgtccgggt ggttatacgc gtattctcag
    35101 gacgggtttc cgcttgggag ataatgcaga aatgtgcatt attgagcttg ttgattacaa
    35161 cgaaaacatg ctcaaggata aaactaccaa gaaaacaact cgtacacgtc gttcttcttc
    35221 tagaaagaaa gcagaaactg aagttcaggc agcacctgcc acttcggaag tttcggctcc
    35281 taaacctgtt gaacaggcac ctgccgaaaa tcaggcacca acgacagaag aataaaattg
    35341 aagatttttt gttaatcata ttaaaaaggg gaccacgcaa gctgttccct tttttgttgg
    35401 gagtaattga ataaaaaact ccctcaaaaa ttaattttga gagagttcaa aaaaactatt
    35461 cttttttttc tgttaaagct ctttatttaa cgctgtccat gtggcagatt aagtcgagaa
    35521 ccttatttga ataaccccat tcgttgtcgt accagcttat cagtttaacg aaattgctgt
    35581 tcaatgcaat accggcattg gcatcgaaaa ttgacgttct gggatcgtga atgaaatcgg
    35641 ttgaaaccac agcatcttcg gtgtaaccca aaataccttt catttcgttt tcagaagcag
    35701 cttttacggc agctttaatt tcttcgtagg tagcagcttt ttccaaacga acggtaaggt
    35761 caactaccga aacatcagca gtcggaacgc gcattgacat acctgtcagt ttcccgttca
    35821 gtgaaggaat aacttttcca acagcttttg cagctcccgt agaagaagga ataatgttgt
    35881 tcaaaataga acggccacct ctccagtctt tatttgaagg agcatcaaca gttttttgag
    35941 tgttggtagc ggcatgaatg gttgtcatta acccttcaac aattccgaaa ttgtcgtgaa
    36001 taactttggc aagtggagcc aagcagttgg tagtacatga agcattggaa acgatatcca
    36061 tatctttggt gtatgcatca agattcacac cgcacacaaa catgggagca tcattcgatg
    36121 gagcagaaat aacaactttc tttgcaccgg ctttcaggtg agcagaagct ttttcaacag
    36181 tggtgaatac accggttgat tctactacat attcagcacc ggcagctccc cagccaatgt
    36241 ttgcaggatc tttttctgca aagaaaagaa cttcttttcc gttgataatt aattttccat
    36301 ccttattttc ggctgttccc tgaaatttgc catgtacgct gtcaaatttc aacatgtaga
    36361 tcagataatc cttatccaaa aaaggatcgt tcacagcaac gacttcaata tcgtttcttt
    36421 cctgggctgc acggaaaacc aaacgtccaa tgcggccaaa accattaata cctactttaa
    36481 tcattttatt taagtttaga gattagttac ttaaaaaacc gtacaaattt acgaattttt
    36541 gtccgatttt caaaggaaca ggttgtttaa ttaaagaaat ttttaagtaa ttggaagatt
    36601 tttttctccc tttccctttt acatgcattt gtatcctttg tcgaatgaaa tgcatgaaga
    36661 taactcaatt tcgtgtaccg aaaattccga ttcctgacgt catcgatgtc ctcagttctt
    36721 gggttgtcgt tttttagtgg gttgttcagc gttttcactc tgctcttttt tctttgctgt
    36781 atttattcca aaaagtgtgt aaagcgggca gaacccaatc agcgaggtta gagtaagtac
    36841 aaaagcgaca ataagagcaa caattcccag aatgacggag aaaataccct tgtaaaacag
    36901 aacaatcagc acgatagcca atgctaagcg tactactttg tcggtcgagc ctacatttgt
    36961 tttcataagt ttgagatttg attttacttt taaaacaaat attcgagccc ttatgtttaa
    37021 attttcatta ccaaatcttg tgaaaatatt tttttggaat gtttttgata gatattttaa
    37081 aatgtttaga acaattaaat aaaaatcagc aagttagaat aattgtttaa attgaaacaa
    37141 tttaaagttt tcttgctgct aaaaagaatt gcagttgaac caatactttt tgtttcgctg
    37201 cagaaaataa gtccaaaatt ttgtgtaagt ttgcatttgg ttcacgaagt tctcggatat
    37261 ttatcatttt aatgagctta tggaaatcaa aaatttaaaa attgccatta ttggagccgg
    37321 aaatatgggt ggagcattgg cacgcggctg gtcaaaatca gacattttaa gtgctgaaaa
    37381 tatcgccatt agcgatattt ctcaagaaaa gttggatcaa ttgactgctt ttaatccaca
    37441 ttttaccgta agtacttcta atagggaaat catcaggcag gccgatattg ttttgcttgc
    37501 tatcaagcct tggctggtcg aagcggttgc caaagaaata agcaagtaca tcgattataa
    37561 aaagcaagta atcatttcca ttgcggcagg cgttagtttt gaaaccctga cgcatttttt
    37621 tgattctaat ggagtgtttt tccgtgttat tcccaatgta gcggtcgaaa taggacaggg
    37681 tgtttctgtt gtttcatcgt tcaatgcctg caaagaacac caacagttat tactggaaat
    37741 ttttaatgtt ttagggaaaa catttttcgt gtccgaaaat caaatggatg cttttatggc
    37801 attaacttca tgtggcattg cttatgcttt tcggtatgtg cgggcttcgg tagccggagc
    37861 tgttgaaatg ggcatttcgc ctcagatggc tcaggaagcg gttattcaaa ccgtaagagg
    37921 tgctgtggat ttgcttgaac tccatcagtc tcatccagaa acggaaatag ataaggttac
    37981 tacgcccgga ggctttacca tcaaaggttt gaacgaaatg gaagccaatg gattttcaaa
    38041 tgctgttatc aaagggttga aagcatctta ttccaaatga aaaaatagat tctgcaaaaa
    38101 acaatttttt ttaagtttta catccatgaa tattcaaatc aaacaaattg ccgaaagatt
    38161 acgtgcattg cgcgaatcac ttgagctgac tcccgaacag gctgcagaaa aatgtggtgc
    38221 tacaacagaa gaatatcaac aatacgaatc gggcgattac gatataccga tgagttttat
    38281 ctgtcaggct gccgattctt tcggcgttgc aactactgct cttcttgttg gcgatgatcc
    38341 tcacgcacag gtttttttcg ttacccgtaa gggaacggga gcaagcgtag aaagaacaaa
    38401 agcttacaaa tatcaggcac ttgcttacgg ttttcgtcat cccaaggcag aaccttttga
    38461 ggttaccgta gaacccaacg acaatccgct tacattgaat acccatttca atcaggagtt
    38521 taattatgtg ctggaaggca ctttgctatt gcgcatcgcc ggcaacgacc ttattctgaa
    38581 cgaaggcgac agcatttact ttgatgcaac acgcccacac ggaatgaaag ctttgaacgg
    38641 gaaaagagtc aaatttctgg ctgttatcat ttaattaata gtctcgaaag aaagcttttt
    38701 tcggataaaa aataatttta aaagacgtag caaattttat taattgaact tatcgttagt
    38761 aatttattct attcaaaata tgttggaaaa atatttaaaa caaactgaat ttaaggattt
    38821 tgatgatttt aaggctaatt atcaattgat cgttcccgag cattttaatt ttgcttacga
    38881 cgtgatagac gagtgggctg ccaaagagcc ggacaaactc gccatgattt ggaccaatga
    38941 tcagggagaa tgcagaaaat tttcttttgc tgaaatgaaa aaatacagtg accagactgc
    39001 ttcttatttc caaagtttgg gtataggaaa aggcgatgtg gtgatggcca tactcaaacg
    39061 tcgatatgag ttctggttta ctattctggc tttacataaa ttgggcgcta ttactattcc
    39121 agacacgcat ttgcttacgc cgaaagactt gatctaccga aacaatgctg ctgatattaa
    39181 ggcaatagtt gcagttggag aagaaccgat tgtaagccac attaacgatt caatggctga
    39241 atcccctact gttgaaatgc tgatttccat tgggcctatc gtgccggaaa attggctcga
    39301 ttttcacaag ggaattgatg aggcgactcc ctttacaccc attcatcatg tgaatgaaaa
    39361 taccgatccg ttgattatca gttttacgtc cggaactaca ggtaatccaa aaatggtgtt
    39421 gcttgattgt ctttatccgt tgggacatat tattacagcc aaatactggc acaatctgca
    39481 tgaaaacagt attcatctga cgatagccga taccggatgg ttgaaagcac tttgggggaa
    39541 aatttatgga cagtggattg tgggtgcttg tgtatttgta tatgaccacg aaaaatttac
    39601 tcccgcaaat atgctcgaaa tgatcgagaa atatcatgta acttctcttt gtgcgcctcc
    39661 aaccattttc cgttttctga tcagggaaga cattagcaag tatgatttgt cttctctgga
    39721 atatgctaca acggcaggag aagccctgaa tgcttccgtt tacgaaaaac tttacgagtt
    39781 gacaggcatt aaactgcacg agggttacgg gcaaagtgaa acgacagttt gtgtatatac
    39841 cactccttgg attgaaccaa aacccggttc gatgggattg ccgggacctc attacgatgt
    39901 ggatattcaa atgccggatg gacgatcggc tgaagtgggt gaacaagggg aaattgtaat
    39961 caagctgcac aaaaattatc ctgtgggttt gttcaaaggt tattatcgga acgaagaact
    40021 gacacgtgaa gctttcaggg gaaatgttta tcataccggc gatatggctt ggaaagatga
    40081 agagggttat ttctggtttg ttggtagaac ggatgatgtg atcaaaagtt ccggttaccg
    40141 tatcgggcct ttcgaagtgg aaagtgcact gatgactcac cctgcagttg ttgaatgtgc
    40201 cattaccgga gtacccgacg aattgcgcgg tcaagtagtg aaagcaacgg taatacttgc
    40261 caaagaatac aaggataaag caggagagga gcttgtaaaa gaacttcaaa atcatgtgaa
    40321 aaaagttacc gctccctata aatatccccg tattatcgaa tttgtggacg aactgcccaa
    40381 aaccatcagc ggaaaaattc gccgtgtgga aataagagaa aaggataaac agtaaaaaaa
    40441 ctgttgtttt gatggagaga gagaagagaa aataattatt tgatgtttgt cgatatatga
    40501 aaattataaa tctgtccgat aacaattcaa ttttaaatca gtttataaaa gaaatccggt
    40561 cggtagaaat tcagaaagat tccatgcgtt tcaggcggaa tatggagaga attggggaaa
    40621 tcatggctta tgaaatcagc aaagctatgg attatgtgga ggaaaaagtt cagactcctt
    40681 tagggattgc tcccgtgaaa gttatcgacg aaaaagtggt aatttctact attcttcgtg
    40741 ccggccttcc tttccatcaa ggttttttaa attatttcga tggctcggaa aatgcttttg
    40801 tctcagctta cagaaaatac aggaatgcgg aaaattttga aatttttgta gaatacattg
    40861 cttctcccgg tttggaagga aaaacgttga ttctcactga tcccatgctg gctaccggaa
    40921 gctcgatgga gcagactttt tcagcgctaa ccaccaaagg atttccctct aaaattcata
    40981 tcgctacggt tattgccagc cgccatgcga taaattatct cactacccat ttccctgaaa
    41041 atataaccac tatctgggct gctgccatcg atcccgaaat aaacagtcat tcttacatca
    41101 ttcctggttt gggggatgcc ggagatttgg cttttggcga gaaactgtaa ataaaaaagc
    41161 tcttcacctg tcgatgaaga gctttaaaaa ttcatttttt atcggttcac gccagcttgt
    41221 tcacatgaat agccaatttc gattttaagt tgttggcttt gttcttgtga atcatattgc
    41281 gtttagccag cttgtcgagc atggaagaaa cttgtggcaa caattctgcg gctttttctt
    41341 tatcggtagt tgtacgcaat cttttaattg ccgtacgtgt tgatttgatg taatagcggt
    41401 tgtgaagcct ttttttgttt gcctgacgaa ttcttttaat agctgaatta ttgttagcca
    41461 ttgttttttg agatattaaa gtgttgtaat atggtagccc atagcagaat cgaactgctc
    41521 tttcaagaat gaaaatcttg cgtcctaacc gatagacgaa tgggccaaaa atgttatgaa
    41581 aacttcaact gttttgcaca attgcgggtg caaatatacg gcagattttt gaaatatcaa
    41641 agactttgtt ttaaattttt taatttttca gcgatatggg ttgttgcttg attttaaatg
    41701 tcgttttgaa acgaattttt tttgtttttg gggaaggacg gatgctaagt tcaacttaaa
    41761 agtgagaatt tagcattaag ttttgcattg gtttcttcat cgaatcaggt aaaaaacgag
    41821 atttttcccg taattttgtt ttcaaaaaaa ggagcgagct gtgcaagtca aaaccgaagg
    41881 tatagttttt cattcactaa agtactcgga caattctgcg atagtaacca tttttacccg
    41941 tcgttttgga cgagttgctt acatgattta tggcagaggg aaaagaaagt cttcgtggaa
    42001 acctgctttt tttcaacctt tgtcgatagt agagctggaa gtttcgcacg atatgcgaaa
    42061 gaatattcag caaatgaagg atgtaagggt gcttgtgcct taccagagcc tgaccatcga
    42121 tcctctcaag cgttctattg ccatgtttat cgccgaagtg cttttgaaag tgctttctca
    42181 aagcgaagcc gatgaatcac tctacctttt tctgcggcaa tcattggagt ttttggataa
    42241 cagtaagcaa aaaacggcga attttcatct ggtattttta cttaaacttt cgcgatactt
    42301 gggctttgaa cccaatatcg atggacagac gacacttttt ttcgatttgg taaacggtgt
    42361 ttttttgtcc tctccgcctg ctcatcggca ttatctcttt gccgaagaag ccgggaaaat
    42421 ggctcagttg ttgcaaaccg actatgagca catgaccgaa atcaaacttt cacgtcagca
    42481 gcgagaaaaa ttattggagg atatagtgag ttattatcgt tttcatcttc ccgattttcc
    42541 cgagattcat tcactggagg tgctgaaaac tctttttgat taatggcgtg tgtcaatatg
    42601 ggaattattg actttttaat tttttttgcg gatacaaaaa agctatttcc gacaaccttc
    42661 ttcaaaagtc gtttatccga tttattttga atttttccga ataaatattc ctgtaagata
    42721 tagcagattc ataaattttt ccgttaattt gtgcagaaat caatttaaaa atactattat
    42781 tatggaaaaa tcaaatgaaa taaccatgga aaaacttttg gctgaactgg aaaacttgaa
    42841 agccaaaaca acgcaattgg aaaattcagc caaaaaagac caattgtcga tagcaattat
    42901 ctcaggcgac atggacaaaa ttatggcagg aatgattata tcgctggcgg cggcagctat
    42961 ggatacaaag gtaaaactgt tcttctcatt ctggggattg gcagcattac gtgatcccaa
    43021 gaaaaccgtg aaaggaaaaa actttgtttc caaaatgttc ggttgcatgt tgcccaaagg
    43081 atatgggaaa ctgaaattgt ccaaacacaa tatgtgtggt atgggaccca tgatgatgaa
    43141 aaatttgatg aaaaaacagc atgtactttc attggacgaa atgtttaagg aagccgccga
    43201 attgggaata gaaattacgg cttgcgaaat gaccatgaat ttgatgggct tcaaaaaaga
    43261 agaattcatt gattatccac atttccgaat tgcaggagca gcaacttttg tggcagatgc
    43321 cggcgaaagc gccatgcaat ggatgatata agcaataact cataaacaaa cattaaaaat
    43381 tatagaatta tgaccaccga agaattgaaa aaccttaccg tagccaaatc ggtagatgca
    43441 cgcggaactg cctgtcctgg accacttttg gaagcaaaaa aagcaattgg aaccatacaa
    43501 tcgggagaaa ttttggaagt tttgtcggcc gatgaaggca caaagaatga tttgccaaaa
    43561 tggtgcaaca agcaaggaaa cgagtttctt ggcatggtag aggaaaacgg atattataaa
    43621 ttgtatctga aaaagaaata atttttttct ccatgacgag cttcgtaaag aaaaatgcaa
    43681 aaattctggt attttctacc gagaaaattt cagatccggc tattgatatg gccgggctgc
    43741 ttaaactgca ttatccgcca accgtttata ccataactgt tccatgttca agcggaataa
    43801 agcccaattg gatcctgcat gcttttgaaa aaggcttcga tggcgttttc attgctgctg
    43861 atggtaccga ttgcgttttt ggcgaaagct gcaccgaaaa aaccgggaaa gtagtggcaa
    43921 tgacccacga aaaaatgaaa cagaacaatt tggatcctgc ccgcttgaga atggctgctc
    43981 tttgctccgt ttgtagcgat gcttttgtca ggcaagtgca tagtctgaat gaatatttga
    44041 ttaaaacccg ctattcggag aaataaacaa tggaggagag aagaaaaaaa atagaattcg
    44101 atgcgctggt tattggtgcg ggtattgccg gcggcgaaac tgccttgaat attgcaaaca
    44161 acggttacaa ggtgctattg gtggaaaggg atcattctat cggaggtaaa atgattttgt
    44221 tgagcaaagt ttttccaaca ttggattgcg ctgcctgtat taccacaccc aaagtttctg
    44281 aaattgcccg tcatcccaat attacagttt ttactgaaag tgaagttaca agtattgtaa
    44341 aaaaaaccga atcgttattt atagcaaaga ttacaaagaa tccgcgttac gttcatgtgg
    44401 aagactgtac ggcttgccaa ttgtgtgaaa aagcttgtcc cgttagtgtt cgggatgaat
    44461 atcagtttgg tctggtcggt cgaaaagctg cctacattcc tttcagcata gccaatccca
    44521 aagctgctgt tatcgacctt gaacaatgta ctttgtgcgg cgcatgcgaa agggtttgcc
    44581 ctactcactg catcgacttt actatggaaa cggaatattt tgaattacag gtaaagacag
    44641 tcgtactggc tacaggtttt caactgtttg atccgcttaa aattccacga ttcggctatg
    44701 gaaaatacaa aaatgtggtt acttccatgc aaatggaaag agaaatagct cctaccagac
    44761 cttatcacac tattctgaga ccgggcgacg gaaaaatacc cgatcagatt gcttatgttc
    44821 tttgtaccgg ctcgcgcgac gacagcgttg gcaatcctgt ttgctcacaa gtatgttgca
    44881 tgtattccat gaaacaggca caattgttaa tgggtgcgct tccaatggca gacatcacca
    44941 tttattacat tgatattcgt tctttcggca aaggctttga agaattctac cagcaagcca
    45001 aagatatggg tgtcaatttt gtaaaaggaa aaattggaaa aatttccgaa aaagaaaatg
    45061 ggaatttaat actgcgttat gaagatattc aggaaggaat agtgaaagaa gccgaacacg
    45121 atatggtggt attgtcggta ggaatgttgt ccaatcagtc agttcatcaa atttttgatg
    45181 gcgaaatgtt gcaatttgat tctttgaatt tcatttggca gagcgacttg ttgggaagtc
    45241 ctgcaaaaac aaatatagat ggagttttcg ttgccggcac ggcatctggt ccaatggata
    45301 ttcccgattc cattctatct gccggttcgg cttcgtccga agcaatcagt tacttaattc
    45361 aacattaata agattgatga aaaagaattt aggcgtttat atctgtcatt gtggtggaaa
    45421 tatttccgat tatgtcgacg tagaaaaagt acgtcaggca attgaacatg aagagggtgt
    45481 cgttttagcg aaaaccacca tgtttgcatg ttcggatagc aatcagaagg aaatggtgga
    45541 agatattcat caatatcagt tggatggagt catagtggca tcctgctctc caaaactgca
    45601 tttggttact tttagaaatg taactgaaac agccggtttg aacaaatata cctacgttca
    45661 tgccaatatt cgtgagcagg cttcttgggc acactcggac gataaagaag gagctaccga
    45721 aaaagcgatt cgactggtaa gggcagccat ttcaaaatcg cgttacgcag aagctttgta
    45781 ccccaataag tttgaagcag agaaagcaat tgcagtcatc ggagcaggaa tggcgggaat
    45841 gaaagcagct ctttccattg ccgaaaaaaa tatgtctgta gtgctgattg aaaaagaaaa
    45901 tactgttgga gggcatgtgg ctgaatgggg atcgcttttc atgacaaatg aaaagggttc
    45961 ggagcttgtt caacgtattc ataacgattt gctgcaacac gaaaatattt ctattctcac
    46021 aggtaccgaa gtcgtttctt caaaaggcag cattggaaat ttcacgctga ctcttcaaaa
    46081 aaaacatcag cctgttgaca atcttagaga aactatggaa attaacgtag gttctgtagt
    46141 gattgctaca ggttttgatc attatattcc gcaacgaggc gaattttcct atctcaataa
    46201 cgagaatgta atttctttgc ccgattttaa aaaactgaca gatcaatcga caggtaaaac
    46261 acttttatac aacggaaaag aaattcacga cattgcttat atctattgcg tgggaagtcg
    46321 tcaatccgaa ggtgagaata aatactgttc gcgtttctgc tgtatttcga caattcatac
    46381 tgctatcgaa acaaaaaaga aattcaaggg gatacgcaat tttcatcttc atcggggtat
    46441 tcgcacttac ggcaaacagg aattacttta tgacgaagct ttacagaatg gggatatttt
    46501 tatccagttt catgatgatt ttcctcctga agtcagtttc aagaataaaa aaacttccct
    46561 gaaagttaaa gatttactgt tttccgataa agaacttctt ctggatgccg atttagtggt
    46621 gttggtaacc ggaatgttac ccagaattga tcagtcgatc ggtcaacttt ttaaactgcc
    46681 aaaaggcaga gataatttct acaacgagat tcacatgaaa ttgcgtccgg tggaaacggt
    46741 gattgatgga ataacactgg ccggaacttg ccaagggcca aaaaatatca gcgaatcggt
    46801 caattcggcc ttgtctgctg ccacaaaggc cttttcatcc atcagtaacg gattggtaga
    46861 aacggagccg atagttgctg aaattaacgg taattcatgt gtttggtgcg gtgaatgttt
    46921 aaaagcttgt ccttttgaag caattgaaca aaaagaaatt gatgggaaaa taattgctgt
    46981 agtcaacaaa tcggtatgca agggttgcgg tatgtgcgct cctgtatgcg tacccaatgc
    47041 tattgatttg attggctatt ccgataagga gatttttagt atgatagatg cactggttca
    47101 ataaaaaaat gatggaagca gaaataggaa aaacggccaa atatttaagg gacaaacaag
    47161 gtattcccga aaaggcaaaa gaaaatctta aagaatttgt caggattaaa aaagcactca
    47221 ttgatgccct gaaaaatgga gaaatgacca ttccccaact atcggaaaaa ctatccatgt
    47281 caaaagagga tgttacattt tatttgatga cgctgattaa atatggcgac gttgcagtgg
    47341 gagaaattga tgacaatgac gaatatttta cctataaatt gaacaaatga tggcatcaaa
    47401 aattaatcct gcatttcagg ataaattgaa gaaatacgga gcagtaaatt tcaacgcctg
    47461 ttacaattgc ggtacctgta ctgctgtatg ttcactgtcc acacctgaag aatcttttcc
    47521 tcgcgaaatg gtaagactta gcagtcttgg acttgaaaat gatattaaat cttctttaaa
    47581 accatgggaa tgctattatt gtggtgaatg taccgaaaat tgtcctcaaa cagccaatcc
    47641 gggagaactc atgatgtcct tgcgacgatg gttaacttca gtttatgact ggacgggact
    47701 ttcgggtttg ctttatcgtt ccgttacatt cactatttct gctctgataa tggtatttat
    47761 tgccgtactt gttttgggta atgccatgtc atttcaattg gcagaaatga tgcattatgg
    47821 acatcttttt gaaatgtatg tggtttttgc agtgctcgca ataattttta tccccaatat
    47881 agccagaatg tggaatttta ccattttaag accaaaagta aaagcaaatc taaaaattta
    47941 cataaaatca ttaccggatc tgtttgttca catgttcact caaaaacgcg cccgagattg
    48001 tgataaagaa gataattttc ggtggctgga gcattttatt ctggttttgg cttatctttc
    48061 gctgcttttc acaaccgtat ttttagattg gtttggcaca aaaagtttct ttgtagtagt
    48121 gttgggttat gtggaaagtt tttttgtatt tgtcattact gccgattttg tttcaagtcg
    48181 aattaggaaa gcaaaggcac tgaacaaatt ttcgcagcct tccgactggc tgtttgttat
    48241 atggcttatg cttatggggc ttacagcttt cctggtacgc gtatttatcg atacccatat
    48301 gatagacaat aatgtatgga tgtatatcgt tcatttaacc gttttggctc aatgggctat
    48361 aattattgtt ccatttggaa aatggtctca tttcctgtat cggtcttttg gcatgtattt
    48421 ttcaagcata aaagcagctt cagaaaaacg aaaaggttaa aaaatcgttt ctgtagaaaa
    48481 atacaaatag atttttttag aaaagaaaga cagctttcaa catttctgtc gtgaagttca
    48541 aatcattatc catcaatgtt attcaatctt ttaccgaatt tattgacatt tggaaaata
//
LOCUS       DAOTAX010000007        48453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_596_length_48453_cov_31.888239, whole genome shotgun sequence.
ACCESSION   DAOTAX010000007 DAOTAX010000000
VERSION     DAOTAX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 48453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48453
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_596_length_48453_cov_31.888239"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..921
                     /gene="pgeF"
                     /locus_tag="PLM04_01595"
     CDS             130..921
                     /gene="pgeF"
                     /locus_tag="PLM04_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HON01807.1"
                     /translation="MRLFIQSEILQPFEEITHFFTTRHGGVSGGEYTSFNLSPYVGDE
                     EENVHRNFEILSRQSEIGTSDIIFPYQVHDCKIREIDRSFFGLSHEQRQHFLNGVDAL
                     FTQLPGVVISITTADCVPLLFYAPEKKVIAVAHAGWRGTCAGIAAKTIETLVGKFDLS
                     PEDIFVAAGPSISPSVYEVGREVVEEFQKNDWNLSDIFSQNDGKIFLNLWKANEISLL
                     GSGLQKNNIEFLCRCTFTESENFFSARRQGILCGRMLSGIMLKQA"
     gene            926..1585
                     /locus_tag="PLM04_01600"
     CDS             926..1585
                     /locus_tag="PLM04_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HON01808.1"
                     /translation="MQIYISELIRQACPDLTLSVIACEVKNSETTSILWQEIEEEMEK
                     ISSTFTLENIKMRPEISATRQVYRKLGKDPNRYRPSAEALCRRICKGVGIQRVSTLVD
                     IINLISIRSGFSIGGFDAKKIQGDLRLEVGTANDIFQAIGRGILNVEGLPLFKDAAGG
                     IGTPTSDNERTKLTMETRRLLMIINGYSGEKGLQEASEQARMLLEKYAEAKEVNITLV
                     K"
     gene            complement(1586..1789)
                     /gene="xseB"
                     /locus_tag="PLM04_01605"
     CDS             complement(1586..1789)
                     /gene="xseB"
                     /locus_tag="PLM04_01605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HON01809.1"
                     /translation="MTKNKPTYDEAIAELDKILAELENNSDINMDEIAQKVKRASELL
                     QICKKQLHVLDENLEKLLSELEE"
     gene            complement(1833..3611)
                     /locus_tag="PLM04_01610"
     CDS             complement(1833..3611)
                     /locus_tag="PLM04_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HON01810.1"
                     /translation="MDWKEIYLQAINWREKHIKERQFILILSFLTGIFAALAAFVLKM
                     AIHSVQSLVTESFNRESLNYLYLITPAVGIIITSLFVRHIVKDDISHGVTRILYAISQ
                     RKSILKLHNVWTSLVGSSITIGFGGSVGAEAPIVLTGSAIGSNLGKFFKMNQKTMMLL
                     IGCGAAGAIAGIFKAPIAGLVFTLEVLMLDMTMTSIVPLLISSVTATSVTYILTGDEF
                     MFQFTNYEPFAVERIPYLLMLGVVCGLVSLYFTRGMNWLENFYKKFENPYIKWAIGGS
                     ILSVLIFLFPPLYGEGYDTIASLLNNDISSVVKQSFFLGSENKPLVLLTYLVLIIVFK
                     IFATASTTSAGGVGGIFAPSLFVGCITGFVVAEILNLIGLNMPIENFALAGMAGLMSG
                     VMHAPLTGIFLIAELTGSYYLFMTLIIVSTISYLTIILFEPHSLYAMRLAQKGELITH
                     NKDKAVLTLLKMENVIETNLNVLEPDMKLGEVIKIISHSKRNVFPVVNPKNGVLLGIL
                     TLDEIRNIMFRPELYQRFTVRQLMISPPAFLNVNMPMEKVMQIFEDTGAWNLPVVDDE
                     KHYIGFVSKSTIFNSYRHVLVHFSDE"
     gene            4106..5488
                     /locus_tag="PLM04_01615"
     CDS             4106..5488
                     /locus_tag="PLM04_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HON01811.1"
                     /translation="MTNVIKTTKGLDIPIDGAAQKMIERTVISDIVALVPDYYHGIIP
                     KVLVRAGDRVKAGTPVFHDKIHENLNIVSPVSGKVLAVNRGEKRKLINITIERDVVNE
                     YENFGHPSLESLSGEEIKSALLKAGIWPYIKQRPYDVIANPNKTPKSIFISSFDTAPL
                     APDYNFILKGQFADFQTGIDALAKLTPGKVHLGVKANVPSNEFLKVKNVEITEYEGPH
                     PIGNVGIQINHVDPINKGEVVWTVNVQDMLYIGRFFNKGIIDLKKIIALTGPEVIEPK
                     YYTTLPGTAVAPFINGNVHKGIPLRYILGNVISGIKVDINDFIDPYASQIFVIDEGTE
                     IHEFLGWAMPRLNKFSATKLFFTPLLQKKFFKKIFGDIKFSYDARILGGRRAIIMSNE
                     YDRVLPMDILPEYLFKAMITGNIDKMEALGGYEIAPEDVALCEFVCTSKLPLQLIVRE
                     ALDMMKKELE"
     gene            5547..6755
                     /locus_tag="PLM04_01620"
     CDS             5547..6755
                     /locus_tag="PLM04_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HON01812.1"
                     /translation="MNALRNFMDKIKPYFEKGGKLEKLHSVYDGFETFLFVPTTTAPK
                     YGSHIHDPVDTKRTMSTVIVALVPALLFGMYNVGYQHYLAIGQDVNFWTEFLFGFLAV
                     LPYIVVSYAVGLGIEFIGAQIKGEEIQEGFLVTGFLIPLIIPINTPLWMVAVATAFAV
                     IFAKEVFGGTGMNIFNVALVARAFLFFGYPTHMSGDSVWIRTGTALGFGGGNVVDAFS
                     GATPLGQIATASTSVPHITDTFGHSLGFWDGFVGLIPGSVGETSIIAIMLGAFVLLIT
                     EVASWKTMLSVFFGGALMGIVFNLFGPDTAAAHFPWYNHLVFGGFAFGAVFMATDPVT
                     SARTEKGKWIYGLLIGCLAIIIRVLNPGYAEGMMLAILLMNIFAPLIDFYVVDANVKK
                     RISRAQKSIQ"
     gene            6803..7528
                     /gene="nqrC"
                     /locus_tag="PLM04_01625"
     CDS             6803..7528
                     /gene="nqrC"
                     /locus_tag="PLM04_01625"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HON01813.1"
                     /translation="MNTNSNTYTLVYATVMVVIVALMLAFVSGALKEKQNSNIELDKK
                     KQILKSLRIDAKQNNIAQLYDQYVIKGLIVNTNGEILSYSKKEAFDIDIAKELSKKVE
                     ERRLPIFIAQVEGETKYIISLRGTGLWGPIWGYVSLNDDKNTIYGAYFSHASETPGLG
                     ANIAEPNFQRQFEGKHIFNEKNEFVSIAVMKVGKKAEGREQVDAISGGTITSKGVETM
                     LLNCIGQYEAFFRQLSTIGEIKQ"
     gene            7525..8157
                     /locus_tag="PLM04_01630"
     CDS             7525..8157
                     /locus_tag="PLM04_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HON01814.1"
                     /translation="MSTLLSKKSRQVLLGPLSKNNPITIQVLGICSALAVTAKLEPAL
                     VMGISVTIIVAFSNIIVSLVRNTIPNRIRIIVQLVVVAALVTIVSEILKAFAYDVSVQ
                     LSVYVGLIITNCILMGRLEAFAMSNSPWESFLDGLGNGFGYAIILIIVSFFRELFGSG
                     TLLGYNIIPQSFFNVGYMNNGLMMMPPMALILVACIIWVHRAKNKELQEK"
     gene            8229..8846
                     /gene="nqrE"
                     /locus_tag="PLM04_01635"
     CDS             8229..8846
                     /gene="nqrE"
                     /locus_tag="PLM04_01635"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HON01815.1"
                     /translation="MESILNIFVKSIFVDNMIFAYFLGMCSYLAVSKNVKTSLGLGTA
                     VTFVLFITVPVNYLLQNYVLKEGALQWLSPQLASLDLSYLSFILFIAVIAAITQIVEM
                     AVEKFSPSLYSSLGIFLPLIAVNCSIMGASLFMQQRAFHNIGEATSYALGSGVGWLLA
                     IVGLAAIREKMEYSNVPKPLRGLGITFITVGLMGIAFMCFSGLKI"
     gene            8955..10214
                     /gene="nqrF"
                     /locus_tag="PLM04_01640"
     CDS             8955..10214
                     /gene="nqrF"
                     /locus_tag="PLM04_01640"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HON01816.1"
                     /translation="MLLAIQTSTLIASLLVFFVVIFLLVVVLLVAKQYLVASGNVTIT
                     INNEKKLEVPAGGTLLSTLVNQQIYLPSACGGKGTCAQCRCQVLEGGGEILPTETGHF
                     SRKEIKEHWRLGCQVKVKNDLSIKIPESILSIKKWECEVISNKNVATFIKELIVKLPE
                     GEHMNFLPGSYIQIDIPPYELKFSEFEVEERFRPDWDKMHLWDLTCSNDEETMRAYSM
                     ANYPAEGDIIMLNVRIATPPIDRATGKFLDVKPGIASSYIFRLKPGDKVNVSGPFGEF
                     HPIIDSKREMLYIGGGAGMAPLRSHILHLLKTLRITDRKISYWYGARSKNEIFYEEDF
                     RELEKEFPNFSFHIALSEPLPEDNWTGPVGFIHSVILNEYLKFHEEPEEIEYYMCGPG
                     PMAKAVEKMLDDLGVPREMLMFDDFGA"
     gene            10434..14225
                     /gene="dnaE"
                     /locus_tag="PLM04_01645"
     CDS             10434..14225
                     /gene="dnaE"
                     /locus_tag="PLM04_01645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HON01817.1"
                     /translation="MNTFVHLHVHSHYSILDGMSSIDQLVDKAMKTGMPAIALTDHGS
                     MFGIKEFYNYVKKKNVPVYENISLLQKQLNKDELSDIQKDEIHKNIETEKRKLFKPII
                     GCETYVAHRSRFSKESQEDRSGYHLILLAKNKTGYRNLCKLISLAWIEGMYYRPRIDH
                     EILEKYSEGLIASSACLGGEIHKKIESGNLEEAEKAVLWYKKVFGDDFYLELQRHKTD
                     KPGGDTSTYEKQTLQNVELIKLARKTNTKLIATNDVHFVEEEHAEGHERLICLSTGKD
                     MDDPTRLRYTKQEWLKTPQQMQEIFSDIPEALENTLEIAQKVEFYDIDSAPMMPTFKI
                     PEEFADENQYRSRYTEAMLREEFGNGFDRLGGMDKVIRIKLEADYLRNLTFEGAKERY
                     GENLDPELEERINFELNVIKTMGFPGYFLIVQDFIAAARNMGVAVGPGRGSAAGSVVA
                     YCLKITDIDPLKYDLLFERFLNPDRISMPDIDVDFDDDGRNEVLRWVTEKYGKDRVAH
                     IITFGTMAAKSAIKDVARVQKLPLSEAERLTKLIPDKLPDTPDGKKMKVNLSNCFKFV
                     PELEIARNSSDENLSSTLRYAEMLEGTVRQTGVHACGIIIGADDLTKFVPLSTAEDKD
                     TKEEILVTQYEGSVIEEVGLIKMDFLGLKTLSIIKDTLENIRKSKNIEVDLDKIPLDD
                     PETYQLFSKGQTVGTFQFESAGMQKHLQALQPTQIEDLIAMNALYRPGPMQYIPQYIN
                     RKHGLEKIEYTFPIMEKRLKETYGITIYQEQVMLLSVDLAGFTRAEADELRKAMGKKL
                     TGKMQALKEKFIAGCQKNGYGPLEKLEQIWSDWVEFSNYAFNKSHATCYSYLAYRTAY
                     LKAHFPSEFMAANLSRNKDDIDEVSKFMDECRNMGINVLGPDVNESDFTFSVNEKNDI
                     RFGLGGIKGVGENAVMAIVNERHAHGKFKNIFDFVERVNLTACNKRAIESLAIAGAFD
                     GLNDIKREQFFVENGKGETVIESLIRYGNKYQADRLMSQNTLFGDMNSIEINKPEIPY
                     APAWAPLNRLNRERELIGMFLSAHPLDQFEFEMNYICNTSTGQLKDLTSLKGKQLKIG
                     GLVTNIRHSVSKAGKPYGVFTLEDYDGSYEFVLYGNNYIEFQKYMIKDMFVFIQAIVQ
                     EKYADSKYRKNTDIGGEQELELKIQKIDFFSDIKDKFVNNLKINIPLQQLTEDTVEML
                     SKIILGNKGNVNVYIQVHDQNSFNKINFFARQHRVDINPKVYRMLKTAKSEGILDFDV
                     N"
     gene            14349..14663
                     /gene="trxA"
                     /locus_tag="PLM04_01650"
     CDS             14349..14663
                     /gene="trxA"
                     /locus_tag="PLM04_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HON01818.1"
                     /translation="MVIEFTDENLNEFIESGKPVLVDFWAEWCGPCRMIGPIVEELAE
                     EYDGKVQVGKLNVDENAEVPTKFGIRNIPTLLFFKDGQLVDKQVGATSKSVLAEKIKS
                     LI"
     gene            complement(14684..15397)
                     /locus_tag="PLM04_01655"
     CDS             complement(14684..15397)
                     /locus_tag="PLM04_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HON01819.1"
                     /translation="MSHPFFQFKQFTVFHDRCAMKVGIDGVTLGAWTPVDNAKKILDI
                     GTGTGLIALMLAQRSTAEIDALDIDKQAIEQARFNVENSAWKNRIRLFETSLQEYHSV
                     CTTAYDVLVCNPPYFINSKKSPIVARNMARHAESLTHEELIDLSFDLLKPDGTLCVIL
                     PVEEGLQAISHALASNWYAHQMVFVFPKPAAKAKRLLIQLGKKEKITRISRLAIETEQ
                     RHEYTDEYKSLVKNFYLKL"
     gene            complement(15404..15919)
                     /locus_tag="PLM04_01660"
     CDS             complement(15404..15919)
                     /locus_tag="PLM04_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01820.1"
                     /translation="MFTTIEISAQEIGNLPEKTPEQEAMQQTSKLQQELDLNAEQVKK
                     IYEINLRYAKQRQSVTKRSEVMELLKRKDGEIENALGKEKYQQLQNKRNERISIDVPT
                     TSGMQNITPSYRSTKRTGNTNLSFPERNLQNDSKTSDGNTEQPKRLTPNNSRRTTDEV
                     ERKNTPPQQRK"
     gene            16847..17338
                     /locus_tag="PLM04_01665"
     CDS             16847..17338
                     /locus_tag="PLM04_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HON01821.1"
                     /translation="MKRIGILSDTHGYLDPRIYQYFSECDEIWHAGDWGSLEIVNQLN
                     EFKPLRGVWGNIDDVNIRSLFPQHFHFQCEDVAVWLTHIGGYPGKYDRSVRPAILQQP
                     PDLFVCGHSHILKVQYDEKLNLLLINPGAAGKYGFHTVQTIVRLEIDGKKIQNLEVIE
                     LEK"
     gene            17399..17986
                     /locus_tag="PLM04_01670"
     CDS             17399..17986
                     /locus_tag="PLM04_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HON01822.1"
                     /translation="MFQVNDTLISENLLEEFFVCNLVSCKGICCVEGDAGAPLEKDEL
                     KIIKDILPEIQEFLPENSKKIIREKGFYYIDQDGDCVTSLVNERECVFAFFEEDGTCK
                     CSLEKAYYQGKTDFCKPISCHLYPVRLQKINDYTAVNLHHWKVCECAFVLGKKLKIPA
                     YRFLKVPLIRRFGEKWYKEVELIADLLKKTRERND"
     gene            18260..19504
                     /locus_tag="PLM04_01675"
     CDS             18260..19504
                     /locus_tag="PLM04_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HON01823.1"
                     /translation="MNSKKVEIITIGDEILIGQIVDTNSAWMAQELNKAGFEVFQITS
                     VHDEASHITNALDEALKRTDIVLITGGIGPTNDDITKKTLCEYFHTKLVFDPSVIENI
                     KQVFANRIDFVFNDLTYSQAMVPENCTVIQNKVGTAPITWFEQDEKVVVSMPGVPYEM
                     MEAMRNEIIPRLQKKFQTQKILHKTIQVFGYSESALAIKLADWEKELPDFIHLAYLPN
                     YGIVKLRLTGTSDDLLSLEFAVNQQIESLKQLLGKAIIAFEDLPIEQIVGNLLTAQGK
                     TTATAESCTGGNIAHRFTSHAGSSEYFKGGIVAYANEVKTEVLQVSSEDLNLYGAVSR
                     QVVEQMARGALQLLHTDTAIATSGIAGPSGGTAEKPVGTVWIAACSKDKMTSRLFHFG
                     NTREQIIQRSTQAALVMLKEIT"
     gene            19827..21020
                     /locus_tag="PLM04_01680"
     CDS             19827..21020
                     /locus_tag="PLM04_01680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HON01824.1"
                     /translation="MFKVSERLAALSPSETLAMSQKSNELKAQGFDVINLSVGEPDFN
                     TPDHIKAAAKQAVDNNYSFYSPVPGFLELRKAICSKLKNENNLEFTPDQIVCSNGAKQ
                     AICNVLLTILNKDDEVIIPAPYWVSYPEMVKLGEGKSVFIYSDIENDFKISPEQLEKA
                     ITEKTKALILCSPSNPTGSVYTKEELQGLAQVLAKHPQILILSDEIYEHINYVGKHES
                     IAQFEEIRDRVVVINGVSKGYAMTGWRIGWIAAPKWIASACGTLQGQYTSGACSVAQK
                     AAEAAYTGDQSCVETMRAAFERRRNLVVELVRQIPGLKVSVPQGAFYVFPDCSAYLGK
                     SFNGKVIENSADLAMFLLETAHVASVGGVAFGAPQCIRFSYATSEDNLKKAFERIQQA
                     LNKLQ"
     gene            21255..21674
                     /gene="ybeY"
                     /locus_tag="PLM04_01685"
     CDS             21255..21674
                     /gene="ybeY"
                     /locus_tag="PLM04_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HON01825.1"
                     /translation="MIQYFTENIPMPALKKQKINRWIKQTAHNYEKKVGEIAYIFCNN
                     ERILEINRQYLQHDYYTDIITFDDSTDSTISGDIFISLETVKSNAEDFSVDFEEELHR
                     VIIHGILHLCGQDDITPELKAEMTRKENKALEELKKM"
     gene            complement(22073..22819)
                     /locus_tag="PLM04_01690"
     CDS             complement(22073..22819)
                     /locus_tag="PLM04_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HON01826.1"
                     /translation="MSESFLSTVVALFLGSVGSVLLAGLILLLKERQLHRVTHVLTYL
                     AGGTLLGSALLGMIPEASDMMEIKKVLYVVLAGIIFFFVLEKIILWRVCSDASCERNL
                     QTAAPMVLFGDAFHNAIDGVIIASAFLTSNTFGWVVTLAVIFHEVPQELGDFGILLKS
                     GMKRSRALLYNALSGASALIFGIVAYFSLNFAKSLIPYALAFSAAGFLYIALADLIPE
                     MHRKTSFKESMVQIFFILFGIMLIYFFGRD"
     gene            complement(22864..23262)
                     /locus_tag="PLM04_01695"
     CDS             complement(22864..23262)
                     /locus_tag="PLM04_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HON01827.1"
                     /translation="MSRPRQKRIIKSPPLMMGYKPFGIPHKELDKIELHFDGFEAIRL
                     LDYEGMTQEEAAGHMNVSRPTLTRIYESARKTIAQALVEGKMIVIKGGNIEFEHQWFR
                     CKYCYQLVKEGETHVHQKYSDGNELLPIES"
     gene            23481..24671
                     /locus_tag="PLM04_01700"
     CDS             23481..24671
                     /locus_tag="PLM04_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HON01828.1"
                     /translation="MVSTTGKSARLLSLDVLRGITIAGMILVNNPGNWGSVYPPLRHA
                     EWNGLTPTDLVFPFFMFIMGVSMYLSYKKFDFKFSNRTFLKLFRRSSLLFLIGLAIGW
                     LGLFSGGLRELQGTDIPLGERIWQAATNFENVRILGVLQRLALVSFFGTLIILLIPHK
                     HIPCLTGAILLIYWILIGVTGSYVASENNIIAIVDRAILGASHMYTETLPDGKVIPFD
                     PEGILSTLPCIAHVLIGFWAGKLISESKSKNEQIQNLAIFGTILFFAGFFLHYGFPVN
                     KKIWSSSFVLVTCGLASLLFSLLIWIIDVNNKKKWSVFFESFGVNPLFIYVVADILSI
                     LLSNVVFMYQGNWISIKNFVYFEWLSSVFGNYLGSLIFALCFVFINWIIAHILYKKKI
                     YIKL"
     gene            24791..25747
                     /gene="tal"
                     /locus_tag="PLM04_01705"
     CDS             24791..25747
                     /gene="tal"
                     /locus_tag="PLM04_01705"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HON01829.1"
                     /translation="MNLLEQLKKFTTVVADTGDFASMMAYQPVDATTNPSLIFSASQD
                     PKYEYLMEDAIRYAQSLTSDKEEQLKKAMDKLAVNFGIKILEIVPGRVSTEVDARLSF
                     DVKGTIAKARELIALYESAGISRERILIKIASTWEGIRAAEILEKEGIHCNLTLLFSL
                     TQAVACAEAGVTLISPFVGRILDWYKKDRGVSDIPPEEDPGVKSVTAIYNYFKKFGYA
                     TQVMGASFRNMGEIIQLAGCDLLTISPALLKELEQTEGVLERKLDPEKAKTADIQPLK
                     INEISFRWMMNEDAMATEKLAEGIRNFTKDLLKLENKLAALL"
     gene            complement(25825..25895)
                     /locus_tag="PLM04_01710"
     tRNA            complement(25825..25895)
                     /locus_tag="PLM04_01710"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25861..25863),aa:Cys,seq:gca)
     gene            complement(25972..26430)
                     /locus_tag="PLM04_01715"
     CDS             complement(25972..26430)
                     /locus_tag="PLM04_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01830.1"
                     /translation="MKKFKYILMLMVWITVNSLSAEENANTNIQKMHEKKWAYIVEQA
                     KLTPDEASKVKPVFMNYENAVWELHEKNRETLNNFLKENKTQPNYELLNDEYVNLQIK
                     QAQLLKNYHLKLKKALSPEKLFHYYQAERTFKRKLLHNMPQGRKMQKNRP"
     gene            complement(26437..26817)
                     /locus_tag="PLM04_01720"
     CDS             complement(26437..26817)
                     /locus_tag="PLM04_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01831.1"
                     /translation="MKKEKNILKKIGNDLPFSVPENYFEQFANEIDRQIGYAKEMEVS
                     KSQKRSFAVISKTWLYMAAMFVGIFLLGNVFYLVFKNTERQKQENYEMYLLSQVDEPT
                     MMYYYMNDAYEAENGTETFQNTSN"
     gene            complement(26922..27494)
                     /locus_tag="PLM04_01725"
     CDS             complement(26922..27494)
                     /locus_tag="PLM04_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HON01832.1"
                     /translation="MENDKNIKGEEKLKEQLQDPKRKAAAFSQLVRMYQERLYWHIRK
                     MVLSHDDANDILQNTFMKAWNGIDNFRGDSLVSTWFYRIATNETLTFLANKRMKNINS
                     LDEVEDVLLQQLQADQYFNGNEAQLKLQQAILTLPEKQRLVFNMRYFDDLSYEQMENI
                     LGTTVGALKASYHLAVKKIEKFLLDSETEI"
     gene            complement(27554..28510)
                     /locus_tag="PLM04_01730"
     CDS             complement(27554..28510)
                     /locus_tag="PLM04_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HON01833.1"
                     /translation="MRVLVSTRLPEEGFSELMKKFEVVFPEKEIFSKEEVIQIIPEFD
                     AFIPTFQFKVDRDVIGAGAQGKLKIIANYGVGYDNIDVDFATKCGIVVTNTPDPVVEP
                     TAEMAIALMLSVARRVSECDRKLRTDNSIKWGVMENLGISLYGKTLGIVGLGRIGKAV
                     ARRAAALGMKIIYFNRNPLDKKTETKLKARYAPLGQLLCESDVISLHVPLTESTFHLI
                     NENTLRLMKPTAILINTSRGPVIDESALTEALTNHKIFGAGLDVFEHEPTIPENLKKL
                     DNVVLSPHNGTATIEDRNAMSRFASQNILRFFEGNKNISRVN"
     gene            28732..29976
                     /locus_tag="PLM04_01735"
     CDS             28732..29976
                     /locus_tag="PLM04_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HON01834.1"
                     /translation="MAIVKPFRGIRPPREIVEKVASRPYDVLNSEEAREEAKGNPMSL
                     YHIIKPEIDFPEGTDEHDERVYAKAAENFKKFRENGWLVQDEEEHFYIYAQTMNGRTQ
                     YGIVVCAHIDDYLNGHIKKHELTRSDKEEDRMKHIRVTNANVEPVFLAYPSNEELNGI
                     VSEIVKSEPVYNFVAPDGFGHHFWKIDDKEIIHLISEIFQKIPSLYIADGHHRSAAAV
                     RVGDEKRRQNPHHTGKEEYNYFMAVCFPDNQLQIMDYNRVVKDLNQLDDEEFLQKLSQ
                     YFIVEKMGTEIYRPNKLHNFSLYLSGNWYSLTAREGTYNDNDPIEGLDVTISSKYILD
                     KILGISDLRTDKRIDFVGGIRGLGELKKLVDSGKMRVALALYPVSMQQLIAIADTGNI
                     MPPKTTWFEPKLRSGLVIHELS"
     gene            30059..32068
                     /locus_tag="PLM04_01740"
     CDS             30059..32068
                     /locus_tag="PLM04_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HON01835.1"
                     /translation="MQFFIKKPSFIFYRIISLCLLLLFLQSCGVSSRIKKADSRFELG
                     EYYAAGDLYKSSYGRIPNKDKSLRGYVAFRQGECYRLINNQRAEQAYLNAIRNNFADS
                     TVFLRYAQVLHRNGKYGEALRNYQLYLQKDSSCIIAKNGIRAIQMLEEWQKNATRYVV
                     KYDNQFNVRRASTFSPAFVNTAADELVFTSTRSTDKKATAKADPITGLPKNNLFLARK
                     NATGKWGKPELLEGEINTPETDDGVCSFAANGKTIYFTRAQQSDLVDMGTKIYVSSRA
                     GATWSPPQMLKIFSDSSISVAHPAIAPDGQTLYFVSDKKDGLGGKDIYRATLEGTDCK
                     YIENLGPEINTPGDEMFPVVRADGTLYFSSNGHPGFGGLDIFKAVPQKNGGWKVENMG
                     APVNSKADDFGMTFAGESESGYFSSNRNEMRGYDALWSFHLPELIYLVEGKVFDDQGQ
                     TISDATIRIVGSNGMNARIQTKKDGTFRIKLEKDVDYVMLATARGFLNQKNELSTQGL
                     TDSKTFNVNFKLSSISKPVKIENIFYEFGKWDLTPASEKSLQELVKLLKDNPNITIEL
                     SSHTDYIGDNAFNKNLSEKRAQSVVNYLIKAGIDKERLTAVGYGEEKPIVVDESLAQK
                     YPFLKVGETLTESYILTLTPEQQEIANQINRRTEFRVLKTTYNLY"
     gene            32149..32943
                     /locus_tag="PLM04_01745"
     CDS             32149..32943
                     /locus_tag="PLM04_01745"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HON01836.1"
                     /translation="MKQYLDLLNRVLTEGVHKEDRTGVGTLSVFGHQMRFSLDEGFPC
                     LTTKKLHLKSIIYELLWFLRGDTNVKFLQENGVSIWNEWADEHGDLGPIYGYQWRSWP
                     DYEGGYIDQIAQAVEAIKNNPNSRRIIVSAWNVADLPKMKLPPCHAFFQFYVADGKLS
                     LQLYQRSADIFLGVPFNIASYALLLMMMAQVTGLKAGDFVHTLGDAHIYTNHLEQVKL
                     QLTRTPRPLPTMLINPEVKDIFSFQYSDFQLINYDPYPHIKGEVAV"
     gene            33051..33551
                     /locus_tag="PLM04_01750"
     CDS             33051..33551
                     /locus_tag="PLM04_01750"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HON01837.1"
                     /translation="MPKISIIAAVADNYAIGNLNKLPWHLPADLKYFKKLTTGHAVIM
                     GKHTFENLPNGPLPNRKNIVLTSVLSDGICEGYFEANSIEEALELCEKEDEVFIIGGV
                     SIFKQTIDLADKMYITWIHGDFQADAYFPKINFEEWEEISREDHPADEKNPYPYTFSV
                     YQRKKK"
     gene            complement(33630..34628)
                     /locus_tag="PLM04_01755"
     CDS             complement(33630..34628)
                     /locus_tag="PLM04_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HON01838.1"
                     /translation="MENITYKALVVEEDGNRYSRKIKELTTEHLFSYGDVLIRVYYSS
                     LNYKDALSATGNKGVTKNYPHTPGIDAAGVVIASNNEKFQSGEKVIVNGYDLGMNTPG
                     GFGEYICVPSDWIVKLPEGLTLKESMIIGTAGFTAGISLTRLTELVSPDMGEIIVSGG
                     TGGVGSMSLSMLAKLGYQTVAITGKITEKSFFETIGCHKILPRSEFESLPQKPILSAK
                     YAGGIDTVGGGILENIIKQIQPLGAVTTCGSIHSTELNISVFPFILRGISLIGVSAQN
                     YPMNLREKLWKKLAADWKPEQLLNLYTEISLPQLSEAIDNMLQGKLKGRILVNLTA"
     gene            complement(34702..35070)
                     /locus_tag="PLM04_01760"
     CDS             complement(34702..35070)
                     /locus_tag="PLM04_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HON01839.1"
                     /translation="MAKEVAQNSGIIYNSLTNGSKIVGTITAENDFRIDGEVEGEIFC
                     NGKVVIGQKGYLKGSISCNNAEIIGTVEGEISVQDTLSLRQTAKVTGNVKINVLIIEP
                     GAIFNGQCCMNETDSIQESE"
     gene            complement(35512..35841)
                     /locus_tag="PLM04_01765"
     CDS             complement(35512..35841)
                     /locus_tag="PLM04_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HON01840.1"
                     /translation="MDFKKMNVPSTTITRDINLIGEKVGNIYEAVVIISKRANQISLE
                     IKSELDKKLQEFSAMGDNIEEVFENREQIEISRYYEKLPKPTLIAIEEFLEDKIYYRN
                     PIKEKLK"
     gene            complement(35855..36661)
                     /gene="bamD"
                     /locus_tag="PLM04_01770"
     CDS             complement(35855..36661)
                     /gene="bamD"
                     /locus_tag="PLM04_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HON01841.1"
                     /translation="MKKYFLVFSILIFLFSSCSEYQKLLKSTDIELKYNKAIEYFNNG
                     DFARATTLFEDISSYYRGTDRSEDILNFLGQSYLGQKDYYTASNYYQTYLKSYPKGRY
                     AENAKFLVGYCYYKDSPDPRLDQTATYQAIASFQEFLDLYPESNKVPQVNKLLDELHD
                     KLAYKYYMNARLYYNLGNYMGNNYLSAVITAQNALKKYPSSSYRDDLSFLILQAKFAQ
                     AQQSILAKKEERYQDTIDEYYSYINEFPNGKFKKQANNIFNDCKKAINLK"
     gene            complement(36752..37741)
                     /locus_tag="PLM04_01775"
     CDS             complement(36752..37741)
                     /locus_tag="PLM04_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HON01842.1"
                     /translation="MFLIATGIFFLIKNQSSSKYIFNEGKIHGTYYHAIYMHPQGIDL
                     QEKIEERMKKIDMSLSTFEPQSVISRINRNDDSVLTDDDFETVFKTAQKISEITYGAF
                     DITVAPLVNAWGFGFDSHDRILQPNLDTIMPFIGYQKIRLENHKLIKDYPKIMLDASA
                     IAKGYSTDIAANVLEENGCKNYLVEIGGEIVCKGKNTKGEKWKIGIDKPIDDETNTLN
                     EIQTVISITDKAMATSGNYRQFYIKDGKKYSHTIDPRTGYPVNKNILSATVIAPNCIT
                     ADALATAFMVLGVDSSLMICRTLPEVDCYLIYNDQKGSNKVIYSEGFENYFSK"
     gene            complement(37990..38559)
                     /locus_tag="PLM04_01780"
     CDS             complement(37990..38559)
                     /locus_tag="PLM04_01780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HON01843.1"
                     /translation="MEKEYFVHESSYIDEQVKIGKGTKIWHFCHVQKGAEIGENCSLG
                     QNVNIANNVKIGNNVRIQNNVSVYEGVELEDNVFCGPSCVFTNVKTPRAHYPVHGIYD
                     KTLIKNGASLGANSTVVCGHTVGRSAMVAAGAVVTKNVKDYALVAGIPARQIGWVCEC
                     GERLEEDLVCKKCGKKYQETENGLKEIYL"
     gene            complement(38932..39888)
                     /locus_tag="PLM04_01785"
     CDS             complement(38932..39888)
                     /locus_tag="PLM04_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HON01844.1"
                     /translation="MNKKICVVGGGKWGQNHIKTLYQLGNLGAIVEANAERLNELLKL
                     YPVEGFTELEQALEQDFDGFTVAVPAPLHYEVAKKIIESGKSVLVEKPMTLSAETSKE
                     LVELAQRKGVQLMVGHVLLFHPAIRKIKEIIDSGELGNLYYLYSNRLNLGTVRTEENV
                     FWSFAPHDISVLDYFVGHPAEKIVSKGSKFLQEEVYDVTITQLDYPNNVHAHIFVSWL
                     HPFKEQRLVVIGSKGMVSFDDATAEKEIKFYDKRIDFEKGIPVKVENPTRIIPYEQKQ
                     PLSEELKYFIEHLDKKIEIADGQSGLEVVKVLEEAEKKIQVG"
     gene            complement(39896..41209)
                     /locus_tag="PLM04_01790"
     CDS             complement(39896..41209)
                     /locus_tag="PLM04_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HON01845.1"
                     /translation="MKNELLKKIEDRKIVVGVVGLGYVGLPLAVEKAKAGFKTIGFDV
                     QPQKVEMVNAGHNYIGDVVDSDLDELVKKGMLSATTDFSFVKDVDFIAICVPTPLDEH
                     QQPDISYVKSSAEAIGKFLKKGTIVVLESTTYPGTTEELIKPILEEESGLKCGIDFYL
                     GFSPERVDPGNLIYKTKNTPKVVGAIGEDALEVIAAMYRAVLEGDVFTVSSPAVAEME
                     KILENTYRNVNIGLINELAMLCHKMNINIWEVIEAAKTKPYGFQPFYPGPGLGGHCIP
                     LDPYYLSWKAREYGFHTSMIESSMMINDRMPEYCVERASKILNRSKKALNGSNILVLG
                     IAYKQDIDDYRESPALNVIDLLKSEGVNVCYFDPYIPEYLRHGKVFKGEKELTSALLA
                     KMDLVIVTAAHSNVDYDFVQQHAKYVFDTKNAMKNVKNRTNIEIL"
     gene            41629..42570
                     /locus_tag="PLM04_01795"
     CDS             41629..42570
                     /locus_tag="PLM04_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HON01846.1"
                     /translation="MWKDFFYFSKGQQVGIIVLLVLIFVVSVVRFALPYLIPSNSNAF
                     DTNFVAEAENFKKSLLSLDSIQQAERQRQFEERYAAYRLNYRKDFSKKENHYELFNFD
                     PNTADSATFVQLGLKSYVASNILKYRSKGGSFKNAEDFSKIYGIPSEKFNELLPYIQI
                     DEKFSNPPVNLGEKAVKENPTSLVELNSADTTLLMQVKGIGKYYAKSIVRFRQNTGGF
                     FSVDQLSEIYGMTPENLEKIKPFCTVNPNLIEKIKVNTSSVDRLNKHPYINFYQAKAI
                     YELRRKKGKLHQFGELKNLSEFTEEQLKKIEPYLSFE"
     gene            complement(42777..43622)
                     /gene="lipA"
                     /locus_tag="PLM04_01800"
     CDS             complement(42777..43622)
                     /gene="lipA"
                     /locus_tag="PLM04_01800"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HON01847.1"
                     /translation="MNFQRKPDWIRTKLHSEATFSEVQQIIEKNGLHTICTSGRCPNQ
                     SECWNKGTATLMILGEICTRACRFCNTLTGQPLPPDTTEPERIAQSIRSLHLRHAVIT
                     SVDRDDLPDKGASHWAKTIRAVKIVNPAITLETLIPDFDGETTLLDLVIAEKPDIISH
                     NLETVSRLTPKIRSKAKYQTSLKVLQYVSSCGITAKTGIMVGLGETEDEIFSLMDDAL
                     KAGCSVLTIGQYLQPSRKNIPVHEYISPKKFEEYRQIGLAKGFRHVESGPFVRSSYHA
                     EKHVL"
     gene            complement(43647..44336)
                     /locus_tag="PLM04_01805"
     CDS             complement(43647..44336)
                     /locus_tag="PLM04_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HON01848.1"
                     /translation="MEILYEDNHIIAVNKTVSEIVQGDKTGDEPLSETLKKYLKNKYN
                     KPGNVFLGVTHRLDRPVSGVVLFAKTDKALSRLNEMFQNKEIKKTYWAIVQQKPPKMV
                     DTLEHFLVRNEAKNKTTAFDRPVDNAKKAILHYKVLTSSDYYYLLEIQLETGRHHQIR
                     CQLAKIGCPIRGDLKYGFPRPNSNGGISLHARKIEFVHPVSKNWIKIVAPAPQDDKLW
                     QFFEESQKEKE"
     gene            complement(44389..45159)
                     /gene="rlmB"
                     /locus_tag="PLM04_01810"
     CDS             complement(44389..45159)
                     /gene="rlmB"
                     /locus_tag="PLM04_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HON01849.1"
                     /translation="MTIYKNKRRPEKEMIFGIRPVLEAIQSGKDIDKILLRRDMSNEI
                     SRELFAATKGLHIPVQFVPLEKLNKFTDKNHQGVIAFTSPVTYQRIEDIIPAVYEVGK
                     MPFIVVLDGITDVRNFGAIARTCECAGVDAIVIPAKGGVSITSDAVKTSAGALLSIPV
                     CRENNLYETLQFLADSGIKLIAATEKGEKNYTAIDFNEPTAIVLGSEDEGISPELLQL
                     CNEKVQIPIFGNISSLNVSVAAGILIYEVIRQREQKNQ"
     gene            complement(45184..45588)
                     /locus_tag="PLM04_01815"
     CDS             complement(45184..45588)
                     /locus_tag="PLM04_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01850.1"
                     /translation="MPTEDYLLKYLEKLSRVIAAMLGFRDSGFPEDALKMADSTYREL
                     LNFDLKELSIEPTEKFIETVRKANYSASILDLLVQLTYETANALEQNHQSAEANGFYE
                     KTLELYRLLNEKDKTFSFEREMRIGEIKQKLL"
     gene            45819..46697
                     /locus_tag="PLM04_01820"
     CDS             45819..46697
                     /locus_tag="PLM04_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HON01851.1"
                     /translation="MFSHIGEIAGLMVALCWTMSALFFEKAGHKIGSLTVNVIRLSLA
                     VVFLGITTFFTRGFFFPTDATHYQWFWLGLSGIIGFFLGDLFLFKSYMVIGSRTAQLI
                     MSLCPMLTALIGWFFLDEKLSLKSILGIVICVTGIIIAISNRNLKLNIPFKGFLLAFG
                     GALGQAVGLILSKKGIGNYDPVAATQIRALFGLVCFALFITFLGRWKQVSAAFTNKSA
                     LKSVTVGTFFGPFVGVALSMFAIQHTKTGIASTLMALVPVFIIWPSAVMFKERITIPQ
                     IIGAFVSIAGVSLFFL"
     gene            complement(46764..48101)
                     /locus_tag="PLM04_01825"
     CDS             complement(46764..48101)
                     /locus_tag="PLM04_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HON01852.1"
                     /translation="MKVEQVIDNLKRRFPNEPEYYQAVQEVLESIVDVYNAHPEFEKQ
                     NLVERLVIPDRILSFRVTWVDDKGNIQTNMGYRVQHNNAIGPYKGGLRFHPSVYPGIL
                     KFLAFEQTFKNALTTLPMGGAKGGSDFNPKGKSDAEVMRFCQAFMLELNKIVGPDTDV
                     PAGDIGVGGREIGYMYGMYKKLRNEANGVLTGKGLEFGGSLIRPEATGYGNIYFLMNM
                     LKTKGIHDLKGQVVAISGSGNVATYTAQKALQLGAKVITFSDSNGYIYDPDGIDEEKL
                     AYVFELKNVYRGRIKEYAEKYGCKYVEGARPWSEKCTIALPSATQNEITGEDAEMLVK
                     NGCIAVSEGANMPSTPEAVDVFLKNKILYAPGKAANAGGVSVSGLEMTQNSMRLNWTR
                     EEVDQKLQQIMQNIHEACVKYGTEADGYVNYVKGANIAGFMKVAKAMMAQGII"
BASE COUNT        14939 a         8956 c         9179 g        15379 t
ORIGIN      
        1 gcaatgaatt taccgaattt tcgcatattt tatataatta ttcagcaaaa ttaaattgtc
       61 tttttggcgt aaaatcaaat ttttctttct tctctgagag ataatttact tattataaat
      121 cggttttcaa tgcgtttgtt tattcaatca gaaatactgc agcctttcga agaaataaca
      181 catttcttca caacgcgtca tggtggggta agtggtggtg aatacacttc tttcaatctt
      241 agtccctatg tgggtgatga agaagaaaat gtgcaccgaa attttgaaat tttgtcccgg
      301 cagtcagaaa ttggaacttc cgacataata tttccctatc aggtacacga ttgcaaaatc
      361 cgtgaaattg atcgttcatt tttcggatta tcgcacgagc aaaggcaaca ttttctcaat
      421 ggtgttgatg cactgtttac tcaattgccc ggagtagtta tcagcattac tactgctgat
      481 tgtgtgccat tgctgttcta tgctcccgag aaaaaagtga ttgctgtggc tcatgccggt
      541 tggcggggaa cttgtgccgg aatagccgca aaaaccattg aaacgctggt tggtaaattt
      601 gacctttcgc cggaagatat ttttgtggcg gcagggcctt ccatttcgcc ttcggtatat
      661 gaagtcggca gggaagtggt agaggaattt cagaaaaacg attggaattt gtcggacatt
      721 ttctcccaaa atgatgggaa aatttttctc aatttatgga aagccaacga aatatcatta
      781 ctgggaagcg gattacaaaa aaataatatt gagtttctgt gtcgctgtac attcaccgaa
      841 tcggaaaact ttttttctgc ccggcgtcaa ggtattctat gcggacgaat gttgagcggc
      901 atcatgttaa aacaagcata atccaatgca gatctacatt agcgaactta ttcgtcaggc
      961 ttgtcccgat ttgactttaa gcgttatcgc ctgcgaagtg aaaaattccg aaaccacaag
     1021 tattttgtgg caagagatag aagaggaaat ggagaaaatt tcttcaactt ttacattgga
     1081 aaatataaaa atgcgtcccg aaatttccgc tacgcgccaa gtttacagaa agttgggaaa
     1141 ggatcccaat cgctatcgtc cttcggccga agcgctgtgc agaagaattt gcaaaggagt
     1201 gggtattcag cgagtaagca cattggtgga tatcatcaac ctgatttcca tccgaagcgg
     1261 attttccatt ggaggatttg atgcaaaaaa aattcaggga gaccttcggc tggaagtggg
     1321 aacggcaaac gatatttttc aagcaattgg tcgcggtatt ttgaatgtgg aaggattacc
     1381 gcttttcaaa gatgctgcag gaggtatcgg aacgcctacc agcgacaacg aacgaacaaa
     1441 attaacaatg gaaactcgcc gtctgttaat gattatcaat ggttatagcg gagaaaaagg
     1501 gttgcaagaa gcgtccgagc aggccagaat gttgctcgaa aaatatgctg aagcaaaaga
     1561 agtgaatatt acacttgtca aataattatt cttccaactc tgagaggagt ttttccagat
     1621 tttcatccag tacgtgcagc tgtttcttac atatttgcaa taattcggaa gctcgtttta
     1681 ctttttgagc tatttcatcc atgttgatgt ccgagttatt ttccagttct gcaagaattt
     1741 tatccaattc agcaatggct tcgtcgtaag taggtttgtt tttggtcatc ttattttaaa
     1801 tgttggttgt ttgtcgataa taaattcttc ggttattcat cggaaaaatg taccagaaca
     1861 tgccgataag aattgaatat ggtggatttt gaaacaaatc cgatataatg tttttcgtcg
     1921 tccacgacgg gaagattcca tgctcccgtg tcttcaaaaa tctgcatgac tttttccatt
     1981 ggcatgttca cattgagaaa agccggaggt gaaatcatga gctgacgtac cgtgaaacgc
     2041 tggtacaatt caggtcggaa catgatattt ctgatttcgt cgagcgttaa aatccccaaa
     2101 agaactccat ttttcggatt taccaccgga aaaacatttc gtttcgaatg cgaaattatt
     2161 ttaattactt ctcccagttt catgtccggt tccaatacgt tcaggtttgt ttctattaca
     2221 ttttccattt tcaggagggt caatacagcc ttgtctttgt tgtgcgtaat caactcgcct
     2281 ttttgagcca gccgcatggc ataaagactg tgtggttcga aaagaataat ggtgagataa
     2341 gaaatggtgg aaacaataat cagcgtcata aacaaataat aacttccggt aagctcggca
     2401 atcaaaaaaa tgccggtcag cggagcatgc atcacacccg acatcaaacc tgccataccg
     2461 gccaatgcga agttttctat cggcatattc aaacctatca ggttgagaat ttcggccaca
     2521 acaaaaccag taatacaacc cacaaaaagc gatggagcaa aaatccctcc aacaccaccg
     2581 gcacttgtgg tagaggcagt tgcaaaaatt ttaaatacaa taatcagcac caaataggtg
     2641 agtaaaacca aaggcttgtt ttccgaaccg agaaaaaaac tttgtttgac taccgaagaa
     2701 atgtcattat tcaacaagga ggcgatggta tcgtatcctt cgccataaag aggaggaaag
     2761 agaaaaatca aaacactcag tatacttccg ccaatggccc atttgatgta aggattttca
     2821 aactttttgt aaaaattttc cagccagttc atgccccttg taaaatacaa cgaaaccaat
     2881 ccgcaaacta cacccaacat caacaaataa ggaatgcgtt caacggcaaa aggttcgtag
     2941 ttggtaaatt gaaacatgaa ttcatcgccc gtcaaaatat aggtaaccga ggttgccgta
     3001 accgacgaaa tgagcaaagg tacaatggaa gtcatggtca tgtcgagcat caaaacttcg
     3061 agcgtaaaga ccaacccggc aatgggagct ttgaaaatac ccgcaatagc accggcagca
     3121 ccgcaaccga tgaggagcat catggttttt tgattcattt tgaaaaattt tcccaaattg
     3181 gaaccaatgg ccgaaccggt caaaactatt ggcgcttcgg ctccaaccga tccaccaaat
     3241 ccaatggtaa tggaactgcc caccaatgac gtccacacat tgtgcaactt aaggatgctt
     3301 tttcgttgag agatggcgta aagaatacgt gttacgccgt gactaatatc atcttttact
     3361 atatgcctga caaatagaga tgtaataata ataccgactg caggtgtgat taaataaaga
     3421 taattgaggc tttcacgatt aaagctttct gttaccaatg actgcacgga atgaatggcc
     3481 atctttagaa caaaagctgc caacgcagca aaaattcctg tcagaaaact taatatcagg
     3541 ataaactggc gttctttgat gtgtttttcg cgccaattta ttgcttgtaa ataaatttct
     3601 ttccaatcca tagtttttat gaaaaacaga tggcaaattt acatgataat ttaatttaca
     3661 caaaatttaa tatccttctc ttacccttca ttgcatgttt acgccaaatc catgaaattt
     3721 cctcctacaa attagtaacc ggatttcttc atttgtcttg tctaagaatg cttttggccg
     3781 aatcattcta taatgccgtt taaaaaccaa ttaattctga aaacctttca aattatactc
     3841 aaataataaa attaatccaa ctttatccgc aaaacactgt tcttcgtcta tttattgatt
     3901 cattataata tttctgaaat aaaaatcgta tataacattt acaattttaa agaagccacg
     3961 aaaaattaat gaaaaatttc tccaataaac tctccgtaat aacaataaaa atgtgtaatt
     4021 ttgcaccgtt tttcaggaca aatattttag caaaaaagtt gagggataat tttatccaaa
     4081 attttaaaac caaatttttc tttttatgac taatgttatt aagactacca agggtttgga
     4141 tatccctata gatggtgctg ctcaaaagat gattgagcgt accgtgattt ctgacattgt
     4201 cgcactcgtt ccggactatt atcatggaat aattccaaaa gtattagtac gggcaggaga
     4261 tcgtgtaaaa gccggcacac cggtttttca cgataaaatt cacgaaaatt taaacatcgt
     4321 atcgcctgtt agcggcaaag tattggctgt aaacagaggt gagaaaagaa aattgataaa
     4381 tattacaata gagcgtgatg ttgtcaacga atatgaaaat tttggacatc catcattgga
     4441 aagtttaagt ggagaggaaa tcaaatcagc cttgttgaaa gctggaattt ggccttacat
     4501 caaacaacgc ccctatgatg taatagccaa tcccaataaa acgccaaagt caatttttat
     4561 ttcctctttc gatacggctc cactggctcc ggattacaat tttattttga aagggcagtt
     4621 tgcagacttt caaacgggaa ttgatgcatt ggccaaattg acaccgggaa aagttcattt
     4681 gggcgtaaaa gccaatgttc cttcaaacga atttttgaaa gtaaaaaatg ttgaaattac
     4741 tgaatatgaa ggacctcatc ccataggaaa tgtaggaata caaatcaatc acgtcgatcc
     4801 cattaacaaa ggtgaggttg tttggactgt caatgttcag gatatgcttt acattggcag
     4861 attttttaat aagggcatca ttgatttaaa aaaaattata gctttaactg gtccagaagt
     4921 aattgaacca aaatattaca ctaccctgcc cggaacagca gtagctcctt ttataaacgg
     4981 taacgtacat aaaggtattc ctctgagata tattttggga aatgtaataa gtggaataaa
     5041 agttgatatt aatgacttta ttgaccctta cgcttctcaa atttttgtta tagatgaagg
     5101 aacagaaatt catgaatttt tgggctgggc catgccccgt ttgaataaat tcagtgctac
     5161 caaattattt tttactcccc tgttacaaaa gaaatttttc aaaaaaatct ttggagatat
     5221 caaattttct tatgatgcac gcattttggg cggaagaaga gccattatta tgtcgaacga
     5281 atatgatcgg gtactgccta tggatatttt gccggaatat ctatttaaag ccatgattac
     5341 cggaaacatt gataaaatgg aggctttagg cggttacgaa atagcacccg aagatgttgc
     5401 tttgtgcgaa tttgtttgta cttccaaatt gcctctgcag ctaattgtac gtgaagcttt
     5461 ggatatgatg aaaaaagaac tggaataaaa aaattcataa aaacaaccgt ttaaacggca
     5521 acaaaaaagc agaaataatt ttggatatga acgcattaag aaatttcatg gataaaataa
     5581 aaccgtactt tgaaaaaggc ggcaagttag aaaaacttca ttctgtttat gatgggtttg
     5641 agacattttt atttgttcct actaccacag cacctaaata cggcagccac attcacgatc
     5701 cagtagacac caagagaacc atgagtacag tcattgttgc cttagtgcct gctcttttgt
     5761 tcggcatgta taatgttggt tatcaacact accttgcaat aggtcaggat gtaaattttt
     5821 ggaccgaatt tttattcggt tttttggccg ttcttccgta tatcgtagtt tcttatgcag
     5881 tgggcttggg aatagaattt attggagcac aaataaaagg tgaggaaata caggaaggtt
     5941 ttttggtaac gggttttctt attccgttaa ttattcctat taacactccg ctttggatgg
     6001 tagcagtagc taccgcattt gccgtaattt ttgccaaaga agtatttggc ggaacaggaa
     6061 tgaatatttt taatgtagca ctggtagcca gggcattttt atttttcggc tatcctactc
     6121 acatgtccgg agacagcgtt tggatacgaa ccggaactgc tttgggattt ggaggaggaa
     6181 atgttgtaga tgctttttca ggggcaactc ctttggggca gatagctact gcttcaactt
     6241 ctgttcccca tataacggac acttttggtc actcattggg attttgggat ggattcgttg
     6301 gcctgattcc cggttctgtg ggagaaactt ctataatagc gatcatgctg ggagcttttg
     6361 ttttgttaat aaccgaagtg gctagttgga aaactatgct ttctgtattt tttggaggtg
     6421 ctttgatggg cattgttttc aatctatttg ggcctgatac tgctgctgca cattttcctt
     6481 ggtacaacca tctggttttt ggtggatttg cctttggtgc ggtatttatg gcaaccgatc
     6541 cggttacttc agcccgtacc gaaaaaggga aatggatata cggtttatta ataggatgcc
     6601 tggccatcat tattcgagta ctcaatccgg gatacgcgga aggaatgatg ctggcaattt
     6661 tgcttatgaa tatttttgct ccgcttatcg atttttatgt tgtagatgcc aatgtaaaaa
     6721 aacggataag tagagcacaa aaatccattc aataaagtaa tgatgggatc agcaataacc
     6781 caaaaaaata atatacatta aaatgaatac gaacagtaat acttacacac tggtttatgc
     6841 tacagtaatg gtggtaatag ttgccctcat gctggcattt gtatcgggag cgctgaagga
     6901 aaagcaaaat tccaatatag aattggacaa gaaaaagcag attttaaaat ctttgcgtat
     6961 cgatgctaaa cagaacaata tagcacaact atatgatcag tatgttatta aaggtttaat
     7021 tgtcaatacc aatggcgaga ttttatccta ctcaaaaaaa gaagcttttg atattgacat
     7081 tgccaaagaa ttatcaaaaa aagttgaaga aaggcgactt ccaattttta tagcacaagt
     7141 cgaaggagaa acaaaatata tcatttcttt gcgtggaaca ggtctttggg gtccgatttg
     7201 gggatatgtg tcactaaacg atgataagaa tactatttac ggagcatatt tttctcatgc
     7261 cagtgaaaca ccgggtttgg gtgccaacat tgctgagccg aattttcaaa gacaatttga
     7321 aggtaaacat atttttaacg aaaaaaatga atttgtgtcg attgcggtta tgaaagtcgg
     7381 taaaaaagct gaaggaaggg aacaagtaga tgctatttcc ggtggaacaa ttaccagcaa
     7441 aggtgtagaa accatgctgc tgaactgtat aggacaatat gaagcgtttt ttcgtcaatt
     7501 atcaaccata ggagaaataa aacaatgagt acattattat ccaaaaagtc aagacaagta
     7561 ttattgggac cattaagcaa gaataatccc atcacgattc aagtattggg tatttgctcg
     7621 gcattagcag ttactgcaaa actcgagcct gctttggtaa tgggaatttc tgttaccatc
     7681 atagttgctt tttcaaatat tattgtttca cttgttcgta ataccattcc caatcgtatt
     7741 cgtattatcg ttcaactggt agttgttgct gcattagtta ccatagtcag cgaaatttta
     7801 aaagcttttg cctacgacgt tagtgttcaa ttgtcggtgt atgtcggact gattattacc
     7861 aactgtattt taatgggacg actggaagct tttgccatgt ccaacagtcc ctgggaatct
     7921 tttttggatg gtttaggcaa tggatttgga tatgctatta ttttaattat tgtttcattt
     7981 ttccgtgaac tttttggttc tggcacacta ttgggatata atattattcc ccaatcattt
     8041 tttaacgtag gatacatgaa caacggactg atgatgatgc ctcctatggc gttgattctc
     8101 gtggcatgta tcatttgggt tcatagagcc aaaaacaagg agttgcaaga aaaatagtca
     8161 tataattttt atgactacta aaacgattga ataattattc atattatcaa gaaaacaaca
     8221 aataaaatat ggaaagtata ttgaatatat ttgttaaatc gatttttgtc gataacatga
     8281 tatttgccta ttttttggga atgtgttcct atttggcagt atctaaaaac gtaaaaacat
     8341 ctttaggact ggggacagcc gttacatttg ttctttttat taccgttccc gtaaattatt
     8401 tactccaaaa ttacgtttta aaagagggag ctttgcagtg gcttagtcca caattggctt
     8461 cactcgattt gagttatctc tctttcattt tgtttattgc cgtcatagcg gccataacac
     8521 aaattgttga aatggcagtt gaaaaatttt ctccttcact ctacagctca ttgggaattt
     8581 ttctgccact tattgcagta aattgttcca taatgggtgc atcgttattt atgcagcaaa
     8641 gagcttttca caatattggc gaagctacat cctacgcttt gggttccggc gtcggctggt
     8701 tgctggctat tgtcggactg gcagccattc gtgaaaaaat ggaatactcc aatgttccga
     8761 aaccgttacg tggtttagga ataactttta taactgtagg tttaatgggg attgccttta
     8821 tgtgtttctc gggcttgaaa atttaattta aaatcttctg tttttttatt ttatacattt
     8881 tatttgcaat aggtattaat taatcaataa attattcctt caaaaaaaac gggaaggata
     8941 acgtcaatta aattatgtta ttagcaattc aaacttcaac actgattgcc agcttattgg
     9001 tattttttgt agttatattt ttattggtag tggttttatt ggttgccaaa caatatcttg
     9061 tcgcttcggg aaacgttact atcaccatta ataatgaaaa aaaactggaa gtgccggccg
     9121 gaggaacttt gttgagtaca ctggtcaatc agcagatata tctaccctct gcctgtggcg
     9181 gaaaaggaac ttgtgctcaa tgtcgctgtc aggtactcga aggcggaggc gaaattttac
     9241 ccaccgaaac aggacatttt tcccgaaaag aaatcaaaga acattggcgg ttgggttgtc
     9301 aggtgaaggt aaaaaatgac ctttcaataa aaattcccga atccattctc agcattaaaa
     9361 aatgggaatg tgaagtgatt tccaataaaa atgttgcgac ctttatcaaa gaactgattg
     9421 taaaacttcc tgagggagag catatgaatt ttctgcccgg aagctacatt caaattgata
     9481 ttccaccata cgaactcaaa ttcagtgaat ttgaagtgga agaacgtttc cgtcccgatt
     9541 gggataaaat gcatctttgg gatttaacat gcagcaatga cgaagaaacc atgcgtgcct
     9601 attcaatggc caattatccg gccgaaggcg atatcatcat gctgaatgtt cgtattgcca
     9661 cccctcccat tgaccgagct acaggaaaat ttttagatgt aaaaccgggt attgcttctt
     9721 cttatatttt ccgtcttaaa ccgggcgata aagtgaatgt ttccggcccg tttggcgagt
     9781 ttcacccaat tatcgattca aaacgcgaga tgctttacat cggtggaggc gccggaatgg
     9841 ctcccttgcg ttcccatatt ttacatttgc tgaaaacctt acgcataacc gaccgaaaaa
     9901 tttcctactg gtatggcgca cgttcgaaaa acgaaatttt ctacgaagaa gatttccgtg
     9961 aactggaaaa agaatttccc aatttctcgt tccacatagc tttaagtgaa cctcttcccg
    10021 aagataactg gactggtcct gtagggttta ttcatagcgt tattctcaat gaatatctca
    10081 aattccacga agaaccagaa gaaattgaat attatatgtg cgggccgggt ccaatggcca
    10141 aagcagtcga aaaaatgctt gacgatctcg gcgtcccgcg tgaaatgctg atgttcgacg
    10201 attttggagc ttgaaaaatt ctttaatatt tctttttcag gggcttatta acatacagcc
    10261 cctgatttat ttttcagaca ctgttgaaaa tcaaaagaga ataaatttta tttccttttt
    10321 ttgaaatttt tccttcgata taaatctgtc cacaacaaaa attttactaa ctttaaacac
    10381 ctaaatttaa agtttttcag cttttctaat attcaatact tcacctattt agcatgaata
    10441 cgtttgttca tttgcacgtt cactcacatt attctattct tgatggcatg tcttccattg
    10501 atcaattggt cgacaaagct atgaaaaccg gcatgccggc tattgcactc accgatcacg
    10561 gcagcatgtt cggaataaaa gaattttaca actacgtaaa aaagaaaaat gttcctgttt
    10621 atgaaaatat ttcccttttg caaaagcaat taaataaaga tgaattatct gacattcaaa
    10681 aagatgagat tcataaaaat attgaaacag aaaaacgtaa acttttcaaa ccaatcatcg
    10741 gatgtgaaac ttatgtagcc catagaagcc gtttctcgaa agaaagtcag gaagaccgaa
    10801 gtggttatca tcttattttg ttggccaaaa ataaaacagg ttatcgaaat ctatgcaaac
    10861 tcatttcgct ggcttggata gaaggaatgt attatcgacc gcgtatcgac catgaaattc
    10921 tggaaaaata cagtgaaggt ttaattgctt cttcagcttg tctgggaggt gaaattcaca
    10981 aaaaaattga atcgggcaac ttggaagaag cagaaaaagc tgttctttgg tacaaaaaag
    11041 tatttggcga cgatttctat ctcgaactgc aacgtcataa aaccgacaaa cccggaggcg
    11101 acacttctac ctacgaaaaa caaacccttc aaaatgttga attgataaag ctggccagaa
    11161 aaaccaatac aaaactgatt gccaccaacg atgtccactt tgtggaagaa gaacatgccg
    11221 aaggacatga acgattgatt tgtctcagta cgggaaaaga tatggacgat cccacacgcc
    11281 tgcgttacac caaacaggaa tggttgaaaa cgcctcaaca aatgcaggaa atattttcgg
    11341 acatacctga agcgctcgaa aatacactcg aaatagctca aaaagtagag ttttacgata
    11401 ttgattcggc gcccatgatg ccaaccttca aaattccgga agaatttgcc gatgaaaatc
    11461 aataccgctc acgttacacc gaagccatgc tgcgtgaaga atttggtaat ggatttgacc
    11521 gtttgggtgg catggacaaa gttatccgaa tcaagctgga agccgattat ttgagaaacc
    11581 ttacttttga aggtgctaaa gaacgctatg gagaaaatct ggatcccgaa cttgaagaac
    11641 gcatcaattt tgagttgaac gttatcaaaa ctatgggatt tcccggttat tttcttattg
    11701 ttcaggattt tattgctgcg gcaagaaata tgggtgttgc cgttggcccg ggtcgtggtt
    11761 cggcagctgg ttcggttgta gcttactgcc tgaaaattac cgatatcgat cctttgaaat
    11821 atgatttgct tttcgagcgt tttctgaatc ccgaccgtat ttccatgccc gatattgatg
    11881 tcgatttcga tgacgacgga cgaaatgaag ttttacgctg ggttaccgaa aaatacggaa
    11941 aggacagagt tgcccatatt atcactttcg gcactatggc agcaaaatca gccataaaag
    12001 atgtggcgcg ggttcaaaaa ttgccacttt ccgaagcaga aagattgacc aaacttattc
    12061 ccgacaaatt gcccgatacg ccggacggaa aaaaaatgaa agtcaatttg tccaattgtt
    12121 tcaaatttgt tcccgaattg gagattgccc gaaattcttc cgacgaaaat ctttccagca
    12181 cacttcgcta tgccgaaatg ctcgaaggca ctgtacgaca aaccggagtt catgcctgtg
    12241 gcataatcat tggtgccgat gacctgacaa aatttgttcc attatccact gccgaagaca
    12301 aggacacaaa agaagaaatt ctggttacgc aatacgaagg ttcggtcata gaggaagtcg
    12361 gattgataaa aatggacttt ttaggtctca aaacgctttc cattatcaaa gatacattgg
    12421 aaaacatcag aaaatccaaa aatatcgaag tagatctcga caaaatacca ttggatgatc
    12481 ctgaaaccta tcaacttttc agcaaaggcc aaactgtagg tacttttcaa tttgaatctg
    12541 ccggcatgca aaaacatttg caagctcttc aacccacaca aattgaagat ttaattgcta
    12601 tgaatgcgct ttaccgtccc ggcccaatgc agtatattcc tcagtatatc aaccgaaaac
    12661 atggattgga aaagattgaa tatacgtttc ccatcatgga aaagaggttg aaagaaactt
    12721 acggtattac catttatcag gaacaggtaa tgttgctgag tgtcgatttg gctggcttta
    12781 cacgtgctga agctgatgaa ctccgaaaag caatggggaa aaaactcacc ggcaaaatgc
    12841 aggctttgaa ggaaaaattt attgcaggtt gccaaaaaaa tggctatggg ccactcgaaa
    12901 aattggaaca aatttggagc gactgggtgg aattttccaa ttatgctttc aataagtcgc
    12961 atgccacctg ctactcttat ctcgcttatc gtaccgctta cctgaaagct cattttccgt
    13021 ccgaattcat ggcagctaac ctctcaagaa ataaggatga catcgacgaa gtgagcaaat
    13081 ttatggacga atgccgaaat atgggcatca atgtgctggg tcccgatgtc aatgagagtg
    13141 attttacttt ttcggtcaac gagaaaaacg atattcgttt tggtttgggc ggtatcaaag
    13201 gtgtgggcga aaatgccgtc atggccatag tcaacgaacg acatgcacac ggaaaattta
    13261 aaaacatttt cgattttgtg gaacgcgtca atcttacggc ttgcaacaaa cgtgctattg
    13321 aaagtctggc aatagccgga gcttttgacg gactaaacga catcaaacgc gaacagtttt
    13381 ttgtggaaaa cggcaaagga gaaacggtga tcgaaagctt gattcgttac gggaacaaat
    13441 atcaagccga cagacttatg tcgcaaaata cactgtttgg cgatatgaat tccattgaaa
    13501 tcaacaagcc ggaaattccc tatgcgccgg cgtgggctcc tcttaaccga ttaaacaggg
    13561 aaagagaatt gatcggcatg ttcctttctg ctcacccact cgatcagttt gaatttgaaa
    13621 tgaattacat ctgcaacact tctaccggac aattgaaaga tttgacttca ctaaaaggga
    13681 aacaactgaa aattggcgga ctcgtcacca atatccgtca ttccgtatca aaagcaggaa
    13741 aaccctacgg cgtattcact ctcgaagatt acgatggttc ctatgaattt gtgttgtatg
    13801 gcaacaatta tatagaattc cagaaataca tgataaaaga catgtttgtt ttcattcagg
    13861 ccatcgttca ggaaaaatat gccgacagca aatacaggaa aaatacagat attggcggag
    13921 aacaagaact tgaacttaaa atacaaaaaa tcgatttttt ctcagatatt aaagacaaat
    13981 tcgtcaataa cctgaaaatt aatatcccac tgcagcaact tacggaagat accgttgaaa
    14041 tgttaagtaa aattattctt ggaaataagg gaaatgtaaa tgtatatatt caggtacacg
    14101 atcaaaattc tttcaataaa atcaactttt ttgcccgaca gcatcgcgtg gatatcaacc
    14161 ccaaagtata tcgaatgctg aaaactgcca aaagtgaagg aatactcgat tttgatgtaa
    14221 attgaaaagt caatgtaatt tttaattgat tttttctata gcaaaatagt gtttaattat
    14281 aaaacttttg ttaccgatat ttgttttttg aataaaaaat tattgcaaac ttcaaaactt
    14341 aaaaaattat ggtcattgaa tttacagacg aaaatcttaa cgaatttatt gaaagtggaa
    14401 aacctgtatt ggttgatttc tgggcagaat ggtgtggccc ttgtagaatg ataggtccta
    14461 ttgtagaaga actggccgaa gaatatgacg gaaaagtgca agtcggaaaa ttaaacgtag
    14521 atgaaaacgc tgaagttcca acaaaatttg gcattcgcaa tatcccaacc ttattgtttt
    14581 ttaaagatgg acaactcgtc gataaacaag ttggtgcaac ttccaaatca gtcttggccg
    14641 aaaaaatcaa atctttgatt taattttttc tctgatttaa ctactacagt ttcaaataaa
    14701 aatttttcac cagacttttg tattcatccg tatattcgtg gcgttgctcc gtttcgatgg
    14761 caagtcgcga aatacgggtg attttttctt ttttacccaa ctgaataagc aatcgcttgg
    14821 ctttggctgc aggcttggga aaaacgaaaa ccatttgatg agcataccaa ttgctggcca
    14881 aagcatgtga aattgcctgc aaaccttcct ccaccggcag aataacacac aatgtcccgt
    14941 ccggtttcaa caaatcaaag gaaagatcaa tcagctcttc atgtgtaaga gattctgcat
    15001 gacgagccat atttctggca acaatcggac tttttttgga atttatgaaa tagggtggat
    15061 tacacaccaa tacatcgtaa gccgttgtac aaactgaatg atactcctgc agagaagttt
    15121 caaacaaacg aatgcgattt ttccatgcgg aattttccac gttgaaacgg gcttgttcaa
    15181 tagcttgttt atcaatgtcc aacgcatcaa tttcagctgt acttcgttga gccagcatca
    15241 aagctattaa gccggtgccc gtcccgatat caagaatttt ttttgcatta tctaccggtg
    15301 tccaagctcc aagcgttacg ccatcaattc ccactttcat ggcacaacga tcatgaaaaa
    15361 cagtgaattg tttgaactga aagaaagggt gcgacatgta tctttacttt ctttgttgag
    15421 gcggagtgtt ttttctttcc acttcatcag tggtacgcct cgaattattg ggagtcaaac
    15481 gtttgggttg ttccgtatta ccatctgaag ttttgctgtc gttttgcaaa tttctttcgg
    15541 gaaaactcaa attcgtattt ccggttcttt tggtggaacg ataggaaggc gtaatatttt
    15601 gcatacccga tgtagttggg acatcaatgg aaattcgttc gttgcgtttg ttctggagct
    15661 gctgatattt ttctttgccc agcgcatttt ctatttcacc atctttcctt ttcagcagtt
    15721 ccatgacttc cgaacgtttt gtaaccgact gacgctgttt ggcataccga aggttgattt
    15781 cgtaaatctt tttaacctgc tctgcgttta aatccaattc ctgttgtagt ttactggttt
    15841 gctgcatggc ttcctgttca ggcgttttct ctggtaagtt ccctatttcc tgagcactta
    15901 tttcaattgt tgtaaacaaa caagaacaga aaaatatatg taaaataaat ttgattttca
    15961 tatccacctt tcaaaaaaac agaattaatt cgatgaatta aaaaaatttt tttagtcaat
    16021 tcctcccaaa aaaaattttt caattagaag taaatctata tttatgaaaa acaacaacga
    16081 atgtgccacg attttcaaaa acagcaaaaa tactaaactt ttttgattaa ttttatttct
    16141 tgaaataagc cacaaatgaa aaacagatgt tttacggttg aagggatggt agagttagga
    16201 gttttgagtt ttgagttcag agttcagagt aaaaatttga ttaattattt gataatcagc
    16261 gaataaatat tttggtagag atgtagcatg ctacgtctaa aaagtttcga gtgaggagtt
    16321 ttgagttcag agtaaaaatt tgattaatta tttgataatc agtgagtaaa tattttgtag
    16381 agacgtagca tgctacgtct aaaaagtttc gggtgaagag ttaagagttc agagtataat
    16441 ttttattaat tatctgataa tcagtgtgta aatatttgat agagccattg catgtaaaag
    16501 ctaacgaatt tgaaagtcta tttgatttgt tcattcacag aaaattatcc ttgataaaaa
    16561 attttctcat ccttaaatcg ttaacacaaa ataattataa cgtaagttca acataaaaat
    16621 attttataag caattataaa ttaatctgat aaggttgatg aaaagcggta ttcacatgtt
    16681 tctaaaatca gttgtttgaa atgaggtctt aatattcata aaactatcat caacaacaag
    16741 aataaaactt attaccttat taccttatta attatcaatt atttatataa tttaaacatg
    16801 gaatcgtaat atcgaactct ctttattaca aatatttata aataaaatga aacggattgg
    16861 aattctttcg gacacacacg gttatttgga tccgcgtata tatcaatatt tttccgaatg
    16921 cgacgaaatt tggcacgccg gcgattgggg ttctttggaa atagtgaatc agctgaacga
    16981 gttcaaacct ctacgtggag tatggggaaa tattgatgat gtgaacattc gctcactttt
    17041 tccgcaacat tttcattttc aatgcgaaga tgtggcagta tggctcacac acattggcgg
    17101 ctatcccgga aaatacgata ggtcggttcg ccctgctatt cttcaacaac ctccggattt
    17161 atttgtatgt ggccattctc atattttaaa agttcagtat gacgaaaaac tcaacttgtt
    17221 gctcattaat cccggagctg ctggtaaata tggttttcat acggttcaaa ccatcgtacg
    17281 tctcgaaatc gacggaaaaa aaattcaaaa tcttgaagta atcgaactgg aaaaataaga
    17341 acttgattaa ctttgcatac tgtttatttt gcaatcgaag caaaaaaaat tgaatactat
    17401 gtttcaagtg aatgatacgc ttatcagtga gaatttattg gaggaatttt ttgtttgcaa
    17461 tttggtttcc tgtaaaggta tttgttgcgt ggaaggcgat gctggcgctc ctttggaaaa
    17521 agacgaattg aaaataataa aagatattct tcctgaaatt caggaatttt taccggaaaa
    17581 ttcaaaaaaa ataattcgag aaaaaggttt ttattatatc gatcaggatg gcgattgcgt
    17641 aacctctctg gtgaatgaaa gagaatgtgt ttttgccttc tttgaagagg atggaacttg
    17701 taaatgtagt ttggagaaag cttattatca gggaaaaacc gatttttgta aaccgatttc
    17761 ctgtcatctc tatcctgtaa gactgcaaaa aatcaacgat tacacggccg ttaatctgca
    17821 tcactggaaa gtttgcgaat gtgcttttgt tttgggaaaa aagttgaaaa ttccggccta
    17881 tcgtttttta aaagtccctt tgatacgtcg ttttggtgaa aaatggtaca aagaagtgga
    17941 attgatagcc gacttgttaa aaaaaacaag agagagaaat gattgagtat tggaaattct
    18001 ctgtagaata agcagcttgg aggaagtagt gcgagatacg agattaaaat tttgactaat
    18061 tatttgataa tcagcaaata aatattcagt agagacgtag catgctacgt ctaaaaaatt
    18121 taaagttaaa cgtgaaaatt ttttgataat cagtattttg ttattatgtt gagccgttgc
    18181 atgaaacagc tcaagcaaaa attagacatg aaagatattt tttactgaca aaaaatatac
    18241 ataaaattat acagaaaaca tgaacagcaa aaaagtagaa atcataacca taggagacga
    18301 aattcttatc ggtcaaatcg tggacaccaa ctcggcatgg atggctcaag agctcaacaa
    18361 ggctggcttt gaagtatttc aaattacttc tgttcacgac gaagcctccc atattaccaa
    18421 cgctctcgat gaagctttaa aacgaacgga tattgtgctg ataacgggtg gcatcggccc
    18481 aaccaacgat gatattacca aaaaaacatt gtgcgaatat ttccatacca agttggtgtt
    18541 cgatccttcg gtaatagaaa atatcaaaca ggttttcgcc aaccgaatcg attttgtttt
    18601 caacgatttg acttactcgc aagctatggt gcctgaaaat tgtaccgtca ttcaaaacaa
    18661 agttggaact gcacccatta catggtttga acaagacgaa aaagtggtgg tttccatgcc
    18721 cggtgttccc tatgaaatga tggaagccat gcgtaacgaa attattccac gtttacagaa
    18781 aaaatttcaa acacaaaaaa ttctgcacaa aacgattcag gtttttggat attccgaatc
    18841 ggctttggcc ataaaacttg cagactggga aaaagaattg cccgatttta ttcatttggc
    18901 ctatctgccg aattatggaa tcgtcaaact cagactgaca ggcacatcag acgatttgct
    18961 ttcattggaa tttgctgtca atcagcaaat cgaatctttg aaacaacttt tgggaaaagc
    19021 catcatcgca tttgaggatt tacctataga gcagattgtc ggaaatttgc tgacagcaca
    19081 aggcaagact actgccacag ccgaaagttg tacaggtggc aacatagctc atcgctttac
    19141 ttctcatgcg ggaagttcgg aatatttcaa aggcggaatt gtagcttatg ccaatgaagt
    19201 taaaacggaa gttttgcaag tatcctccga agatttgaac ttatatggag ctgtcagccg
    19261 gcaggtggta gaacaaatgg caagaggagc cttgcagctt ttgcacaccg acacagccat
    19321 agccacttcc ggtattgccg ggccttcggg cggaactgcg gaaaaaccgg ttggaacagt
    19381 ttggatagct gcctgctcaa aagataaaat gacaagccga ctttttcatt ttggcaatac
    19441 ccgcgaacaa attattcaac gttccacgca ggctgcactg gtcatgctta aagaaattac
    19501 ataaaatgca ttatattact gctcaactga tacaaggtaa ttgccgtgtt tgtgtgtgtt
    19561 tgaatctgaa aaaatcactg attcttaatt tttcatgtca atttttttca cgaaaaacca
    19621 taaacctgtt gggtcttggg tacaaaccat aaaaaaagaa aataaaaatt cataaaaaaa
    19681 ttatctattt atcaggaaaa cagatattta attttaatta aaaaaataaa caagaattat
    19741 tacaacaaat tgcctttaaa atcaaataaa ttgtaatttt ttgattattt ttgcatcaaa
    19801 atcactacaa aaaattagaa aaagcaatgt ttaaagtatc agaacgtttg gcagccttat
    19861 caccttcgga aacgcttgcc atgtctcaaa aaagtaacga actgaaggct cagggatttg
    19921 acgttatcaa cctcagcgtt ggtgaaccgg attttaacac tcccgatcac attaaagctg
    19981 ctgcaaaaca agcagttgac aacaattact ccttctattc gcctgtaccg gggtttctcg
    20041 aattgagaaa agctatttgc tcaaaactca aaaatgaaaa caacttggaa tttacccccg
    20101 atcagattgt ttgttccaat ggagcaaaac aagccatttg caatgtgctg ttgacaattt
    20161 tgaacaagga tgatgaagtg attattccgg ctccatattg ggtaagctat cccgaaatgg
    20221 tcaagttggg cgaaggtaaa tcggtattta tctattccga cattgaaaat gattttaaaa
    20281 tttctccgga acaattggaa aaagccatta cggaaaaaac caaagccctg attctttgtt
    20341 ctccttccaa tcctacaggc agcgtttaca ccaaagaaga attgcaggga ttggctcaag
    20401 tattggcaaa acacccacaa atactcattc tctccgatga aatttacgag cacatcaatt
    20461 atgttgggaa acacgaaagt attgcccaat ttgaagaaat tcgagacaga gtggtagtta
    20521 taaacggtgt ttccaaagga tacgcaatga caggctggag aataggttgg atagccgctc
    20581 cgaaatggat tgcttctgcc tgcggaacat tacaaggaca atatacttcc ggagcttgtt
    20641 ccgttgctca aaaagccgcc gaagccgcgt ataccggaga tcagtcgtgc gtggaaacca
    20701 tgcgtgctgc ctttgaacga cgtagaaatt tggtggtgga actggttcgc caaatacccg
    20761 ggttgaaagt tagtgttcca caaggtgcat tctatgtttt tcccgattgc agtgcctatc
    20821 ttggaaaatc tttcaacgga aaggttattg aaaattctgc agaccttgcc atgtttttac
    20881 tcgaaacagc tcatgttgca tcggttggcg gtgtagcctt tggtgctccg caatgtattc
    20941 gcttttccta tgcaacatcc gaagataatc tgaaaaaagc ttttgaaaga attcagcagg
    21001 cactcaacaa acttcaataa tttttttaat ttacaaatac gctgaaaaac ctttttattc
    21061 tttgtttgct caattaattt tatccttttg ttcctcccac tatggaaaag aacaaaagga
    21121 tatttttttt ggaaaaaatg caaaaagaaa aaactaaagt tccttcaaaa cattcttcag
    21181 caaaatcaag tatatttgtc tgaaaatttc ataaatttct tttatgtgaa aacctattta
    21241 tatttcattt agtcatgatt caatacttta ccgaaaacat tccgatgccg gcactgaaaa
    21301 aacaaaaaat caaccgctgg ataaaacaga cagcccacaa ttacgaaaaa aaagttggag
    21361 aaattgccta tatattttgt aacaacgaac gcatcctcga aatcaatcga caatatttgc
    21421 aacacgatta ttacaccgat atcatcactt tcgacgacag tacggattcc acaatttcgg
    21481 gcgatatttt tataagtttg gaaactgtga aaagcaatgc cgaagatttc agtgtcgact
    21541 ttgaagaaga gctccacaga gtcatcattc acggtattct ccatctttgc ggacaagatg
    21601 atattactcc cgaattaaaa gcagaaatga ctcgaaaaga gaataaggca ctggaagaat
    21661 taaaaaaaat gtaagttttg acgaaaaatt tctaaaagtt gcatttttat ttcttggcat
    21721 ctgaaaccat accaatgaaa gactaaaaac ataaggagca ttcatacggt tggttatcag
    21781 taaaataaaa tctttactct aaaacatttt tcccctgctc cctaattttt gtcgttattg
    21841 ttgtatattc cagaaatcaa taaataacac aattttcaaa caaaatttaa ctcttgaatt
    21901 agctgcttaa cctgcaaaaa atgccattgt gattgaccct ctggatttgg aatatttcat
    21961 tagaatataa tttatttttt aattgtctga tggcatccac atgtcggaat attatattca
    22021 ttcgtgtttg tatatgtaag acatgagggt ttcatataaa aatcaatgat tgctaatctc
    22081 ttccaaaaaa ataaatcagc ataattccga acaaaataaa gaaaatctgc accatcgact
    22141 ccttgaatga cgtttttctg tgcatttcag gaatcaaatc agccaaagca atgtacaaaa
    22201 aaccggcagc agaaaatgcc aatgcataag gaatcaaaga cttggcaaaa ttgagcgaaa
    22261 aatacgcaac tattccgaat atcaaagccg aagctcctga taaagcattg taaagcaatg
    22321 ctctggaacg tttcattccg ctttttaaaa gtatcccgaa atcgcccaat tcctgtggta
    22381 cttcatgaaa aattaccgct aaagttacga cccaaccaaa agtattggaa gttaaaaagg
    22441 ctgaagcaat aatcacccca tcaatggcat tgtggaaggc atcgccaaat aaaaccattg
    22501 gagccgctgt ttgaagattt ctttcgcatg aagcgtcgga gcataccctc cataaaataa
    22561 ttttttcgag aacaaagaaa aaaataattc cagcaagtac cacatataac acctttttta
    22621 tttccatcat gtcggaagct tcaggaatca ttcccaacaa agctgatcca agtaatgtac
    22681 ctcctgccaa gtaagttaaa acgtgtgtaa ccctatgcaa ctgccgctct tttaacaaca
    22741 aaattaaacc ggccaacagt acgctgccaa cacttcctaa aaacaaagca acaacagtag
    22801 aaagaaaact ttcggacata aattttattt tttgatttac tacgaaataa atgaattact
    22861 tatctaactt tctatgggta atagttcgtt tccatccgaa tacttttgat gtacatgagt
    22921 ttcgccttct ttgaccaatt gataacagta tttacaacga aaccattgat gctcaaactc
    22981 aatatttccg cctttaatta ctatcatttt tccttcaacc aatgcttggg caatggtttt
    23041 tctggccgat tcgtaaattc tcgttagtgt aggtcgcgaa acattcatgt gtccagctgc
    23101 ctcttcctgc gtcattcctt cataatcgag caaacgaatg gcttcaaacc catcaaaatg
    23161 gagttcaatt ttatccaatt ccttatgtgg aatgccaaaa ggcttgtaac ccatcataag
    23221 cggaggcgat tttattatac gcttttgtcg gggacgtgac atgtttttta tttttcaaac
    23281 tacaaatata taaattattt tgaacttatg ttcgttatta tttattttaa aaaaattcta
    23341 ctaattaact cgcaattctt tcttcacatg atgcttcatg tttgaattat cttttttaat
    23401 ctatgaatat ttaaagaaaa tattttcatt aaatttgcaa caataatttt ccttaaattt
    23461 aactgcttaa aaaattattt atggtatcaa ctacaggcaa gtctgctcgt ttgctttccc
    23521 ttgacgtatt gcgcggcatc accatcgccg gaatgatttt ggtcaataat ccgggtaatt
    23581 ggggaagtgt ttatccaccc ttgcgacatg cagaatggaa cggcttaact ccaaccgatt
    23641 tggtttttcc gttttttatg tttatcatgg gtgtttccat gtatttatcc tacaagaaat
    23701 tcgattttaa attttctaat cgcacctttt taaaattatt cagaaggtct tcattgctat
    23761 ttttgattgg attggctatt ggctggctcg gactgttcag tggcggatta cgtgaattgc
    23821 aaggaacaga tataccattg ggtgaaagaa tctggcaagc agccaccaat tttgaaaatg
    23881 ttcgtatttt gggcgttttg caacggttgg ctttggtatc tttttttggt acattgatca
    23941 ttttgttgat tccgcacaaa cacattccct gtttaacggg agcaatattg ttaatttact
    24001 ggattctgat tggggtcacc ggaagttatg ttgccagcga aaacaatatc attgcgattg
    24061 tggatagagc tatactgggc gcttcacaca tgtataccga aactttacct gacggaaaag
    24121 ttattccctt tgatccggag ggaattttga gcacattacc ctgcatagca catgtattaa
    24181 ttggtttttg ggcaggaaaa ctgatttctg aatcaaaatc gaaaaatgaa caaattcaaa
    24241 acttggcaat ttttgggact atacttttct ttgcaggttt ttttctccat tatggttttc
    24301 ccgttaacaa gaaaatttgg tcttccagct ttgttctagt aacttgtggc ttggcttcct
    24361 tgcttttttc tttgctaatt tggattatcg acgtaaacaa taaaaagaaa tggagtgtgt
    24421 tttttgaatc ttttggggtc aatccattgt ttatttatgt agttgccgat attttaagta
    24481 ttttgctaag caatgttgta tttatgtatc aaggcaattg gatttccatt aagaatttcg
    24541 tgtattttga gtggttatct tctgtttttg gaaattattt gggatcactc atttttgctt
    24601 tatgttttgt tttcatcaat tggataattg cacatatact ctacaaaaag aaaatttaca
    24661 tcaaactttg ataggtgatt ttttttgaaa atgtgataaa ttttcagtta aacaattgaa
    24721 tgtcaataaa tcatttaaat tatctattaa cttattccat tgaaaatttt attttatata
    24781 acttaaactt atgaatttac tggaacaatt aaaaaaattt acgactgtgg ttgccgacac
    24841 gggggatttt gcatccatga tggcttatca gcctgtggac gctacaacca atccttcatt
    24901 gattttttct gcctctcaag atccaaaata cgaatattta atggaagatg ctattcgcta
    24961 tgcccaatcg ttgacttctg ataaggaaga acagttgaaa aaagcaatgg ataaattggc
    25021 tgttaatttc ggtatcaaaa ttttggaaat cgttccggga cgcgtttcaa ccgaagtcga
    25081 tgcacgttta tcgttcgacg taaagggaac aatagccaaa gcccgcgaat taattgctct
    25141 atatgaatca gcaggaattt caagagaacg aattcttatt aaaattgctt ctacttggga
    25201 aggaattcgg gctgccgaaa tactggaaaa agaaggcatt cactgcaatt tgactttgtt
    25261 gttttcattg acgcaggccg ttgcttgcgc tgaagctgga gtaacgttga tttcgccctt
    25321 tgttggaaga atcctggatt ggtacaaaaa agatcgtgga gtttccgata ttccacctga
    25381 agaagatccg ggagtaaaat ctgttacagc catatataat tattttaaaa agtttggtta
    25441 tgccacacaa gtgatgggcg ccagtttcag aaatatgggc gaaatcattc agctcgcagg
    25501 atgcgatttg ttgaccattt cgccggcttt gctcaaagaa ctcgagcaaa cggaaggtgt
    25561 gctggaaagg aaattagatc cggaaaaagc aaaaactgcc gacattcaac ctttgaaaat
    25621 taatgaaatt agttttcgct ggatgatgaa tgaagacgct atggctaccg agaaattggc
    25681 cgaaggaatt agaaatttca ccaaagattt gctgaaactc gaaaataaac tggccgcctt
    25741 attgtgaaaa atggaattat tactgtccat aaaaaagaag caagctcttt tttcgcaaaa
    25801 acgaaagaga gcttgctaaa aatgaggtac ttggcggatt cgaaccgccg tatgcggttt
    25861 tgcagaccgg tgcctaacct ctcggccaaa gtaccgtgtt ttggtctgca aagatagaac
    25921 ttttttcctt cactccaaag ctttccgcag aaaagtagct taataaaaaa cttagggacg
    25981 gtttttttgc attttcctcc cctgaggcat attatgaagc aatttgcgtt taaaagttcg
    26041 ctccgcttga taataatgaa ataatttttc gggtgaaagg gctttcttta attttagatg
    26101 ataatttttg agtaactgtg cctgctttat ttgcaaatta acatattcat cattcagcag
    26161 ttcatagttt ggctgcgttt tattttcctt cagaaaatta ttcaatgttt ctctgttttt
    26221 ttcatgcaat tcccatacgg cattttcgta attcataaat acaggtttca cttttgaggc
    26281 ttcatcgggg gtcagttttg cctgttcaac tatgtaagcc catttttttt cgtgcatttt
    26341 ctggatattg gtgttggcgt tttcttctgc tgaaagtgag tttacagtta tccacaccat
    26401 tagcatcaaa atatatttga attttttcat ttgaaattaa tttgaagtgt tttgaaaggt
    26461 ttcagtacca ttttctgctt cataagcatc attcatatag taatacatca tggttggttc
    26521 gtccacttgt gagagcaaat acatttcgta gttttcctgt ttttggcgtt cggtgttttt
    26581 gaaaaccaaa taaaaaacat tgccgagcaa aaatattccc acaaacatag cagccatata
    26641 gagccatgtc ttcgaaataa cggcaaaact tcttttttgc gatttggaaa cttccatttc
    26701 ttttgcatag ccaatttgac gatcaatttc attggcaaat tgctcgaaat aattttcagg
    26761 aacgctgaaa ggtaagtcgt ttccaatttt tttcaatatg tttttttctt ttttcataac
    26821 tatttgttag actgtaaaaa atggtttagg tttaatttgg aaatgttatt tagtacatca
    26881 tctatcataa atcgataaaa atcaaattgt tatattagtt gctaaatttc agtttcgctg
    26941 tcgagtaaaa atttttcaat tttttttact gccaaatgat aggatgcttt caatgctccg
    27001 acagttgttc ccaatatgtt ttccatttgt tcgtaagaga gatcatcgaa atatctcata
    27061 ttgaaaacca gtctttgttt ttccggcaaa gttaaaatgg cttgctgtaa ttttaattga
    27121 gcttcgttac cgttgaaata ttggtcggct tgcaactgtt gcagcaacac atcttccact
    27181 tcgtcgagcg aattgatgtt tttcatcctt ttgttggcca gaaaagtcag tgtttcgttg
    27241 gtggcaatcc ggtaaaacca ggtagaaaca agtgagtcgc ctctgaagtt gtctattccg
    27301 ttccaagctt tcataaaagt attttgcaga atatcgttag catcgtcgtg tgacagcacc
    27361 atttttcgga tatgccaata caaacgttcc tgatacatgc gtaccagttg cgagaaagct
    27421 gcagcttttc ttttcgggtc ttgcagctgt tctttcagtt tttcttcgcc ttttatattt
    27481 ttatcgttct ccacttcaag ttggtcgggc tactttttct tcattttgtt tgactttgat
    27541 ttgtgccttt cgtttaattg accctggaaa tatttttgtt gccctcaaaa aaacgcagaa
    27601 tattttgtga ggcaaatctg ctcattgcgt ttcgatcctc aatggtagct gttccgttat
    27661 gaggcgaaag aacgacgtta tcgagttttt tcaaattttc cggaattgta ggttcatgtt
    27721 caaatacatc gaggccggca ccaaaaattt tatggttcgt taaagcttcc gtcaatgccg
    27781 attcgtcaat aaccgggcca cgtgaggtgt tgattaaaat ggccgtaggt ttcatcaaac
    27841 gaagtgtgtt ttcattgatc agatggaaag tgctttcggt caaaggtaca tgtaaactga
    27901 tgacatcgct ttcacaaagc agttgtccga gtggtgcata gcgagcttta agttttgttt
    27961 ccgttttttt gtccaaagga tttcggttga aatagataat tttcattccc aatgcagcag
    28021 ctcttcgagc cacagctttc ccgatgcgtc ccagcccgac aattcccagt gttttaccgt
    28081 aaagtgagat acccaaattt tccattactc cccatttgat actgttatct gttctcagtt
    28141 ttctatcgca ttcgctcacg cgtcgtgcta ctgacagcat taaggcaatc gccatttcgg
    28201 ctgttggttc cactacagga tcgggcgtat tggttacaac aatgccgcat ttggtggcaa
    28261 aatcaacatc aatattgtcg tatccgaccc cataattggc aataattttc agttttcctt
    28321 gagcgccggc gccaattacg tctcggtcaa ccttaaattg aaaagttgga ataaacgcat
    28381 caaactccgg aataatttgt atgacttctt cttttgaaaa tatttctttt tcaggaaaaa
    28441 caacttcaaa ttttttcatt aattccgaaa aaccttcttc cggtaaacgg gttgaaacga
    28501 gtacgcgcat attttttaga gaatagttgg ttattgtaaa tggatggaac tcttggtctg
    28561 atcatacaaa attacggaag tgtcaaaaag tatgcaaata tataggttta aattatctat
    28621 ttctttttga atacattgat tggatgacaa ttggtcgtgg acaggaatta taaaataaat
    28681 ttttttgtgt acttttgcga tgaaataata aacttaaaaa agtaatatat tatggctatt
    28741 gtaaaacctt tcagaggaat ccgtcctcca cgcgaaattg tagaaaaagt ggcttctcga
    28801 ccttatgatg tattgaattc ggaagaagca agagaagagg caaaaggcaa tccgatgtcg
    28861 ctgtatcaca ttattaaacc ggaaatcgat tttcctgaag gaacggatga acatgatgaa
    28921 agagtatatg caaaagcagc agaaaatttc aagaaatttc gtgaaaatgg ttggttggtt
    28981 caggatgaag aagaacattt ttacatttac gctcaaacca tgaatggccg tactcaatac
    29041 ggaatagttg tttgcgccca tattgacgat tacttgaacg ggcacatcaa aaaacatgaa
    29101 ttgacacgca gcgacaagga agaggaccgc atgaaacata ttcgggttac caatgccaat
    29161 gttgaaccgg tatttcttgc atatccttca aatgaagaat tgaacggaat cgtttcggaa
    29221 attgttaaat cagagccagt ttataatttt gttgcgcccg atggctttgg acaccatttc
    29281 tggaaaattg acgataaaga aattattcat ctcatctcgg aaatttttca aaaaattcct
    29341 tctctttata ttgccgatgg gcaccatcgt tcagctgcgg ctgttcgggt gggcgatgag
    29401 aaaagacgac aaaatcccca tcataccgga aaggaagaat acaattattt tatggctgtt
    29461 tgttttcccg acaaccagtt gcaaattatg gattacaaca gggtggtaaa agatttgaat
    29521 cagcttgacg atgaagagtt tctacaaaag ctttcccaat attttattgt cgaaaaaatg
    29581 ggtacagaaa tttatcgccc aaataaattg cataattttt cgttatattt gtcgggaaac
    29641 tggtattcgc tgacagctcg tgaaggaacc tacaacgaca atgatcccat tgaaggtctt
    29701 gatgttacta tctcttcaaa atatattttg gataaaattc ttggaataag cgatttgagg
    29761 actgataaac ggattgattt tgtgggagga attcgtggtt tgggtgagtt gaaaaaattg
    29821 gttgatagcg gaaaaatgcg ggtcgctttg gcactttatc ccgtatcgat gcagcaactt
    29881 attgccattg ccgacacggg aaacattatg ccgccaaaaa ccacatggtt tgaaccaaaa
    29941 ttgcgttccg ggctggtcat tcatgagctg agctaaatta ataaaacaat accggtatta
    30001 aattttttaa ttttacgaca ttacaaaatt tttacgagtt gccgattaac aaatatttat
    30061 gcaatttttt ataaaaaaac cgtcttttat tttctatcga attatttcgc tttgtttgct
    30121 tcttttattt ctgcaaagtt gtggcgtcag ttcacgtata aaaaaagccg attcgcgatt
    30181 tgagttgggg gaatattatg ctgcgggcga tttgtataaa agttcgtatg gacggattcc
    30241 caataaggat aaatcattgc gcggatatgt tgcttttcga cagggtgagt gttaccggct
    30301 catcaacaat caacgggctg aacaggctta tctgaatgct attcgaaaca attttgccga
    30361 cagcaccgtt tttcttcggt atgcgcaagt attgcatcga aatggaaaat atggcgaagc
    30421 tttacgcaat tatcagctgt atttgcaaaa agattcttcc tgcattattg caaaaaatgg
    30481 tattcgtgcc attcaaatgt tggaggaatg gcagaaaaat gcaacccgtt atgttgtaaa
    30541 atatgataat cagtttaacg ttcgaagagc ttccactttc agtcccgcat ttgtaaatac
    30601 agctgctgac gaactggttt ttacttctac tcgttctacc gataaaaaag ccacagccaa
    30661 agccgatccc atcaccggtt tacccaaaaa caacctgttt ctggcgcgaa aaaatgcaac
    30721 tggaaagtgg ggaaaacccg aattgttgga aggagaaata aatacacccg aaaccgacga
    30781 tggcgtttgt tcctttgccg ctaacggaaa aaccatttat ttcacacggg ctcagcaatc
    30841 cgatttggta gatatgggta cgaaaattta tgtatcttcc cgagcaggcg caacctggag
    30901 tccacctcaa atgttgaaaa ttttttccga tagttcgatt tcggttgccc atcctgccat
    30961 tgcacccgat ggacaaacac tttattttgt ttccgacaaa aaagatggct tgggaggaaa
    31021 agatatttat agagccacat tggaaggaac cgactgcaaa tacattgaaa atttaggtcc
    31081 ggaaatcaat acacccggcg atgaaatgtt tcccgttgtt cgagccgatg gaactttgta
    31141 tttttcatcg aatggacatc caggatttgg aggattggat atttttaaag ctgttccaca
    31201 aaaaaatgga ggatggaagg ttgaaaatat gggagcaccg gttaattcaa aggcagatga
    31261 ttttgggatg acgtttgccg gtgaaagcga aagcggttat ttttcttcaa atcgcaacga
    31321 aatgagaggt tacgatgctt tgtggagttt tcatttgccc gaattgattt atttggttga
    31381 aggaaaagta tttgatgatc agggacaaac tatttctgat gctacaattc gtattgttgg
    31441 cagcaatggg atgaatgctc gcattcaaac aaaaaaggat ggtacttttc gaattaaact
    31501 cgaaaaagat gtcgattacg tgatgctggc aaccgctcgc ggttttctga atcaaaaaaa
    31561 tgaactttca acacaaggac ttaccgacag caaaactttc aatgtaaatt tcaagctttc
    31621 atctatcagt aaaccggtga aaattgaaaa tattttttat gaatttggaa aatgggattt
    31681 gacgcctgct tcggaaaaat ctttgcagga actggttaaa ttgctgaaag acaatccgaa
    31741 tattaccatt gaattgagtt cgcacaccga ctacattggc gacaatgctt tcaataagaa
    31801 tctttccgaa aagcgggcgc agtcggtggt gaattatttg ataaaagccg gaatagacaa
    31861 ggagcggttg acagcggtgg ggtatggaga ggaaaaaccg atagtggtag atgaatcgct
    31921 ggcccagaaa tatccatttc tgaaagtggg agaaactttg acggaaagtt atatattaac
    31981 tctcaccccc gaacaacagg aaattgccaa tcaaattaat cgtcgtaccg aattccgtgt
    32041 tttaaaaacg acttataatt tgtattgatc tttagcgctg aaaattatta caaatcgtaa
    32101 aaagttgcat tattaacttg aatttgttgc ttattaaaat tttttgggat gaaacaatat
    32161 ttggatttgt tgaatcgtgt tcttacagaa ggagttcata aagaagaccg tacaggagtg
    32221 gggactttaa gtgtttttgg tcatcaaatg cgtttttcgt tggacgaagg gttcccttgt
    32281 ttgactacca aaaaattgca tttaaaatcg attatttatg aattgctctg gtttctgaga
    32341 ggcgatacaa atgttaagtt tttgcaggaa aacggcgtaa gtatttggaa cgaatgggca
    32401 gacgaacatg gtgatttggg cccaatttac ggttatcaat ggcgctcgtg gcccgattat
    32461 gaaggcggtt atattgatca gattgcccaa gcggtggagg ctatcaaaaa caatcccaat
    32521 tcacggcgaa tcattgtcag tgcatggaat gtagccgatt tgccaaaaat gaagttgcct
    32581 ccgtgtcatg ctttctttca gttttatgtg gccgacggca aattgagcct tcaactttat
    32641 cagcggagtg ccgatatttt tttgggtgtt ccctttaata ttgccagtta tgctctgctc
    32701 ctgatgatga tggcccaagt tacaggactg aaagctgggg attttgttca tacgcttgga
    32761 gatgcccata tttacaccaa tcatctggag caggtaaaat tgcagcttac gcgaactcca
    32821 cgtcctttgc caaccatgtt gatcaacccg gaggtaaaag atattttttc ttttcagtat
    32881 tccgactttc agctaattaa ttatgaccct tatcctcaca ttaaagggga agtagctgtt
    32941 tgaaattatt ttctgaaaaa atagtttagg ttttacaata agttttttat aactttgtaa
    33001 gtttgcatgt ttttgtgaga attatcaata tggattaaaa attacctatc atgcctaaaa
    33061 tttcaattat tgctgctgtt gccgataatt atgccatcgg caatttgaat aaactgcctt
    33121 ggcatttgcc ggccgatttg aagtatttca aaaaacttac taccggtcat gcagttatca
    33181 tgggaaaaca cacttttgaa aatttgccca atgggccttt gccaaaccga aaaaatattg
    33241 tgttaacttc ggtgttgtcc gatggaattt gtgaaggcta tttcgaagcc aactccattg
    33301 aagaagctct cgaattgtgc gaaaaagaag acgaggtttt tattattggc ggagtatcca
    33361 tttttaagca aaccatcgat ttggcagata aaatgtatat tacgtggata catggagatt
    33421 ttcaggcaga tgcttatttc ccgaaaatta attttgaaga atgggaagaa atcagcagag
    33481 aagatcatcc ggccgatgaa aaaaaccctt atccttacac attttcagtt taccagcgta
    33541 aaaagaagta attttgcagc aaatttttta tgttaaccgg tcgtctatcg ttatttgact
    33601 ttagacgact tttttattcg taaattttct caggcagtca ggttgacaag aattctacct
    33661 ttcagtttgc cttgcaacat gttatctata gcttctgaaa gttggggaag agaaatttcg
    33721 gtgtaaagat ttaaaagctg ttcgggcttc cagtcggcag ccaatttttt ccataatttt
    33781 tcccgtaagt tcattggata attttgtgcc gaaacgccta tgagcgaaat gccacgtaaa
    33841 ataaagggaa aaacggaaat attcaattcg gtggaatgaa tgctgccaca agtagtaacg
    33901 gcaccgagcg gctgtatctg tttgatgata ttttccagaa ttcctccacc tacagtatca
    33961 atgccaccgg catatttagc tgatagaatc ggtttttgcg gcaaggactc aaattcgctg
    34021 cgcggaagaa tcttatgaca accaatggtt tcaaaaaaac ttttttcggt tattttacct
    34081 gtaatggcaa cggtttgata gccaagtttg gcaagcatgg aaagagacat ggaaccaaca
    34141 ccacctgttc cacccgaaac aataatttca cccatatcgg gagaaaccag ctcagtgagt
    34201 cgagtcagtg aaatccctgc cgtaaatcct gccgtcccaa ttatcatgga ttctttcagt
    34261 gtcaaacctt caggaagctt tacgatccag tcggaaggga cgcaaatgta ttctccaaag
    34321 cctccgggcg tattcatgcc caagtcataa ccgttaacaa taactttttc gcccgattgg
    34381 aatttttcgt tgtttgaagc tataactaca cctgccgcat caattcccgg cgtgtgagga
    34441 tagttttttg ttacgccttt gttgccggtg gcagacagtg catccttgta attgagcgaa
    34501 gaataataaa cccgaataag aacatcacca tacgaaaaca aatgttcggt tgtcagttct
    34561 ttaatttttc ttgagtatcg attaccatcc tcttcaacaa ctaaagcttt ataggtgata
    34621 ttttccattc ccaaatttta tttgatggta aaaattgaat tgttgaattt cgattggttt
    34681 caggaaaata aagcgagttg tttattcgct ttcctgaatc gaatctgttt cattcatgca
    34741 acattgtcca ttgaaaatag cgccgggttc tatgattagc acattaattt ttacgttgcc
    34801 tgtaactttt gcagtttgac gtaaagacaa tgtgtcttgt accgatattt caccttctac
    34861 ggttccaata atttcagcat tgttgcacga aattgatcct ttcaggtaac ccttttgtcc
    34921 gatcactact tttccgttac aaaaaatttc gccttccact tctccgtcaa ttctgaaatc
    34981 attttcggcc gtgattgttc caacaatttt acttccgttg gtcaacgaat tataaatgat
    35041 tcccgaattt tgagcaactt ctttagccat gtttcgaaat aaattttaat tgttttttgt
    35101 tcgcccaaaa atacgtgttt tttttcagaa atcaaattcc aggcgtatgg gtcattcaac
    35161 ccactcaaaa ttttactctt ttttgttttt aatattattt ttttaactga aatggaaagc
    35221 gaagtttggt ggggagagaa acttaataag tgaaaatcaa attgattgat gatagtaagg
    35281 taagctattg agaaaatgtt aagtacaaat cattcacctg cttctcacat ttaataaact
    35341 tattgaatgc atctcgaaaa tcttctaaat tcaactcaat gacctcgttt gttgtttgct
    35401 attagcgtga agctattaat aacaaacaac aaatgaggaa aatatcgaaa taaatttaaa
    35461 tcaatttttt tgaaaaatat tcttcaaaaa cagtagttta aggctgaaac cctattttaa
    35521 tttttctttt atcggattgc gataataaat tttatcttcc aaaaattctt caatggcaat
    35581 caaggttggc ttgggtaatt tttcgtaata acgagaaatt tcaatttgtt cacgattttc
    35641 aaatacttct tcaatattat cacccattgc tgaaaactct tgaagttttt tgtctaattc
    35701 cgattttatt tccaaactaa tttggttggc acgcttgcta ataattacta ctgcttcata
    35761 aatattcccc accttttcgc ctataaggtt tatatctcgt gtgatggttg ttgaagggac
    35821 attcattttt ttaaaatcca taaatattct aattttattt taagttgata gcctttttgc
    35881 aatcattaaa tatgttattg gcctgttttt taaatttgcc gttaggaaat tcgttgatgt
    35941 agctatagta ttcatcaatg gtatcctgat aacgttcttc ctttttggca agaatacttt
    36001 gttgagcttg agcaaatttt gcctgtaaaa ttaaaaaaga aagatcgtcc ctgtatgaac
    36061 tggaaggata ttttttcaac gcattttgag cggttattac tgccgacaaa taattgtttc
    36121 ccatgtaatt tccaagatta taatacaatc gggcattcat ataatatttg taagccaatt
    36181 tatcatgtaa ttcatcgagc aatttgttta cttggggtac tttattactt tcgggatata
    36241 aatccaaaaa ttcttgaaat gaagctatag cttgataggt agcagtttgg tcgagcctgg
    36301 gatcaggcga atctttataa tagcaatatc caaccagaaa ttttgcattt tctgcgtaac
    36361 gtccttttgg atacgatttc aagtaggttt gataataatt ggaagcggta taataatctt
    36421 tctgtcctaa gtacgactga cctaaaaaat tcaatatatc ttccgatcgg tcagttccac
    36481 gataataaga tgaaatatct tcgaataaag ttgtagcacg agcaaaatca ccgttgttga
    36541 aatattcgat agccttattg tattttaatt ctatatcggt gcttttgagc aatttttggt
    36601 actcgctaca tgaagaaaaa agaaatatca gaatagaaaa aaccaaaaaa tattttttca
    36661 taacaagcaa atatattgca aaagtaatta ttttactgag aataaaaaaa tattttgatc
    36721 agcttgcggg aatgatcaac tcctttggtt attattttga aaaatagttt tcaaatccct
    36781 cgctgtatat taccttatta ctaccttttt ggtcgttata tatcaaatag caatctactt
    36841 ccggtaatgt tctacaaatc ataagactgc tatcgactcc caagaccata aaagctgttg
    36901 ctaaagcatc ggctgtaatg caatttggag ctataacagt tgcgctcaat atatttttat
    36961 tgaccggata gcctgttcgt ggatcaatag tatgagagta cttttttcca tcctttatat
    37021 aaaattgtct ataatttcct gaagttgcca ttgccttgtc ggtgattgaa atcaccgttt
    37081 gaatttcatt cagtgtattg gtttcatcat cgattggctt atcaatccca attttccatt
    37141 tttctccttt tgtgtttttt cctttacaca caatttcacc tcctatttcg accaaataat
    37201 ttttacaacc attctcttcg agaacatttg ctgcaatatc tgtggaataa ccttttgcaa
    37261 tagcacttgc atcgagcatg attttaggat aatcctttat caatttatgg ttctcaagcc
    37321 tgattttttg ataaccaata aaaggcatga tggtatcaag atttggttgc agaattctat
    37381 cgtggctatc aaaaccaaaa ccccaagcat taacaagcgg tgcaacagta atgtcgaaag
    37441 cgccataagt aatttcagaa attttttgtg ctgttttgaa tacagtttca aaatcatcat
    37501 ctgtcaagac agaatcgtca tttctgttga ttcgggaaat tacagattgt ggttcaaaag
    37561 ttgaaagaga catatctatt tttttcatgc gctcttcaat tttttcctgt aaatcaattc
    37621 cttgaggatg catatagatg gcatgataat aggtaccatg aatttttcct tcattaaaaa
    37681 tatatttcga cgaagattga tttttaataa gaaaaaatat gcccgttgca attaaaaaca
    37741 aaaagattat taaaaatatt tttcttttaa cactcatttg taaagatctt atttcctttt
    37801 atttttaaat aggttttcaa ttcatttaat gaaaacttaa ctcaattagg actgatcatt
    37861 cgctttaaaa aaaattaaat ttcatcagaa tataatttat tttttaaagg tctgatggaa
    37921 cccacctgtc tgaatattat attcattcgt gtttgtgtat gcaatacatg tgggtttcat
    37981 atcaacaact tataaataaa tttctttcaa tccgttttct gtttcctgat actttttccc
    38041 gcatttttta caaaccaaat cttcctccaa tcgttcgcca cattcgcaca cccagccaat
    38101 ctgtcgggcg ggaatgccgg caacaagggc ataatccttc acatttttgg taacgacggc
    38161 tccggcagct accatagccg accgaccaac tgtatgtccg caaacgaccg tactgtttgc
    38221 tcccagtgaa gcgccatttt taatcaacgt tttgtcgtaa atgccatgaa caggataatg
    38281 agcgcgaggt gtttttacat tggtaaacac acaggaaggc ccgcaaaaaa cattatcttc
    38341 cagttccaca ccttcgtaaa cggaaacatt gttttgaatc cggacattgt taccgatttt
    38401 cacattgttg gctatgttta cattctgccc caacgaacaa ttttcaccaa tttctgcacc
    38461 cttttgcaca tggcagaaat gccagatttt ggttcccttg ccaattttta cctgttcgtc
    38521 gatataactg ctttcgtgta caaaatattc tttttccata aagtattttg tttttattca
    38581 ataatttaag tctttcaata ttgagtaatg agtattgagt gtagaaaaca gagtgttgag
    38641 ttttaataaa atgcaggagc aataacaaat taatttatta taactcacaa ctaatactaa
    38701 taataacttt aaactatgaa ctttaaactc aaaacttgta actctttact cctcacttat
    38761 tacacaaaac tccaaaatat taactcttta aacgtagcat gttacgtctc tactgaatat
    38821 ttactcgctg attttcaaat cattaataaa aaatcttatc caaatcttgc aacgcttgac
    38881 tcttgactct ttactctaaa ctcgaaactc ataactcaaa actactatct cctaccctac
    38941 ctgtatcttt ttctctgctt cttccagcac ttttaccact tccaaacccg attgtccatc
    39001 ggctatttct atttttttat ccagatgttc gataaaatat ttcaattctt cggagagtgg
    39061 ttgtttttgt tcataaggaa tgattctggt aggattttca actttcactg gaattccctt
    39121 ttcaaaatca attcgtttgt cgtaaaattt aatctctttt tcagccgtag catcatcaaa
    39181 ggagaccatt cctttgctac caatgaccac cagccgctgt tctttaaacg gatgaagcca
    39241 cgatacaaaa atatgagcgt gaacattatt cggataatcc agttgagtaa tggtaacgtc
    39301 gtaaacttct tcctgaagaa attttgaacc ttttgaaaca atcttttcag ccggatgacc
    39361 cacaaaataa tccaaaaccg aaatatcgtg cggggcaaac gaccagaaca cgttttcttc
    39421 cgtacgaacc gtgccgagat tcaacctgtt ggaataaaga taataaagat ttcccaattc
    39481 tccactgtca attatctcct taatttttcg aatagccgga tggaaaagca atacatgacc
    39541 caccatcaat tgaacgcctt tcctttgagc cagttcaaca agttctttgg aagtctcggc
    39601 tgacaaagtc atgggttttt cgaccaatac tgactttcca ctttcaataa tctttttggc
    39661 cacttcgtaa tgcagtggcg caggaacagc aacggtaaat ccgtcaaaat cttgttccaa
    39721 tgcctgttcc aattctgtaa atccttctac gggataaagt ttcagcaatt cgtttaaacg
    39781 ttctgcattg gcttcaacga tagcgcccaa attgcccaat tgataaagcg ttttgatatg
    39841 gttttgtccc catttgcccc caccaacaac acatattttt ttgttcattt caatgttaca
    39901 gtatttctat attcgttctg tttttgacat ttttcatggc attcttggta tcgaaaacat
    39961 atttggcgtg ttgctgaaca aaatcgtaat cgacatttga atgagcagca gtaacaataa
    40021 ccaaatccat tttggccagc aatgccgaag taagttcttt ttctcctttg aaaactttac
    40081 catgacgcaa atattcagga atgtaggggt caaaataaca aacattcact ccttccgatt
    40141 tcaacaaatc gataacattc agtgccggac tttctcgata atcgtcaata tcctgtttgt
    40201 aggcaatacc caaaaccaga atattggaac cattcaatgc ttttttagag cgattgagaa
    40261 ttttactggc tcgttctaca caatattcgg gcatgcggtc attgatcatc atagaagatt
    40321 caatcatgga agtatgaaat ccgtactcac gagctttcca cgaaagataa taaggatcga
    40381 gcggaatgca gtgtcctccc aaacccgggc cgggataaaa aggctgaaaa ccataaggtt
    40441 tggttttggc tgcttcaatc acttcccata tattgatatt cattttatga cacagcatgg
    40501 cgagttcgtt tatcaagccg atatttacgt ttcgatacgt gttttcgagt atcttttcca
    40561 tttcggccac agcagggctc gaaacggtga atacatcacc ttccaacaca gccctgtaca
    40621 tggcagcaat cacttccaat gcatcttccc caatagcccc aaccactttg ggcgtatttt
    40681 tggttttata aatcaaattt ccgggatcta ctctttcggg agaaaaaccc aaataaaaat
    40741 cgataccgca tttcaatccc gactcctctt caagaattgg ttttattaat tcttcagttg
    40801 ttcccggata agttgtagat tccaacacaa cgatagttcc tttttttaga aacttgccta
    40861 tggcttcggc agagcttttt acataactga tgtcgggctg ttgatgttca tcgagcggtg
    40921 taggaacaca tatagcaata aaatcgacgt cttttacaaa gctgaaatcg gtagtagccg
    40981 aaagcattcc tttttttacc agttcatcca aatcgctgtc cactacgtcg ccaatataat
    41041 tatgcccggc atttaccatt tcaactttct gcggctgaac atcaaagcca atggttttaa
    41101 aacctgcttt ggctttttcc actgccaaag gtaaacctac ataacccaaa ccgactaccc
    41161 cgacaacaat ttttctgtct tcaattttct ttagcaattc gtttttcata tgctttattt
    41221 aaaattgtca taatttgtat taaggaataa gaaaccaaca tgtattacat acacaaacac
    41281 gaatgaatat aatattcaga catgtgggtt ccatcagaac tttaaaaaat aaattatatt
    41341 ctgatgaaat taccttattt ttcagaaaac tcccatttcg ttaagcatcc acaaagataa
    41401 tacaattcat aaaaaaaaga tgcctttggg aaaatttaca aaaattcact tgttgaaaag
    41461 cactttcgga aaatgacggc ttcacaaatc agaaaatgaa atatatcgcc gccgttttgg
    41521 aataggaaga aagaaaaaga cattcagttt gctgaaaatt tgaaaggtta ataaaaattt
    41581 ttttaaattt gcaacggttt ctaataaaat ttactcaaat tcagcactat gtggaaagat
    41641 tttttctatt tttccaaagg acaacaagtg ggcattattg ttttactggt gttgatattt
    41701 gtggtatcag ttgttcgttt tgctctcccc tatctcatac cgagcaattc caatgctttc
    41761 gatacgaatt ttgttgctga ggcggaaaat ttcaaaaaat cgctgctttc gctggacagc
    41821 attcaacaag ctgaacgtca acgtcaattt gaagaaaggt atgcagctta caggttgaac
    41881 tatcgaaaag atttttcaaa aaaggaaaac cattatgaac tttttaattt tgatcctaat
    41941 actgctgatt ctgctacttt cgttcaactt ggattaaaat cttatgtggc ttcgaacatc
    42001 ttgaagtacc gaagcaaagg cggcagtttc aaaaacgcag aagatttctc gaaaatttat
    42061 ggtattcctt ctgagaaatt caatgaatta cttccttata ttcaaataga tgaaaaattt
    42121 tctaacccgc ccgtcaatct cggcgaaaag gctgtcaaag aaaatccgac ttcattggta
    42181 gagttaaatt ctgccgacac cacccttttg atgcaagtga aaggaatcgg taaatattat
    42241 gcaaaaagca ttgttcgatt tcgacaaaat acgggtggat ttttttctgt cgatcaactt
    42301 tcggaaattt acggaatgac accggaaaat ctggaaaaaa tcaaaccttt ctgcaccgtc
    42361 aatccgaatt tgatagaaaa aataaaagtc aatacttctt ctgtcgaccg tttgaacaaa
    42421 catccctata tcaactttta tcaggcaaaa gccatttacg agttaaggcg aaagaaaggc
    42481 aaactgcatc aattcggcga gctgaaaaac ctgtcggaat ttactgaaga acaattgaaa
    42541 aaaatcgaac cttatttaag ttttgaataa aaaatgattt ctatttggtc tgacgaactg
    42601 atatcttgcg agaaaaacat acttgttgat ttataaaaaa gtaaaatttt tcatataaat
    42661 taactgattt ccaatatttt atgatcaaga tgaaatccaa aaatatactg caggacaatg
    42721 gtaccctcat tacatttaat gaataaccgg aaaaatttct ttatggcaaa gcgtttttac
    42781 aaaacgtgtt tttcggcgtg ataggaagat cgcacgaaag gtccactttc aacatgtcgg
    42841 aatccttttg ccaaaccgat ttgccgatat tcttcaaatt tttttggaga tatgtattca
    42901 tgaacgggaa tattttttcg cgacggttgc aaatattgtc caatggtcag aacggaacaa
    42961 cccgctttca atgcatcatc catcaatgaa aatatttcgt cttcggtttc tcccaaacca
    43021 accatgattc ctgttttggc cgttattccg caggaagaaa catattgcag cactttcagg
    43081 cttgtttggt attttgcttt ggaacgaatt ttaggcgtca atcgactgac agtttcgaga
    43141 ttgtgtgaaa taatatccgg tttttcggca ataaccaaat cgagcaacgt tgtttcgcca
    43201 tcaaaatcgg gaatcagcgt ttcgagggtg atagccggat tgacaatttt caccgcacga
    43261 atggtttttg cccaatggga tgcaccttta tcgggcaaat cgtctctgtc caccgaagtg
    43321 atgacggcat gacgcaaatg caatgatcgt atgctttgag caattcgttc aggttctgta
    43381 gtatcaggag gcaatggttg accggtgagg gtgttgcaga aacggcaggc acgtgtgcat
    43441 atttctccca aaatcatgag cgtagccgta cctttattcc agcattcact ttgattgggg
    43501 cagcgtccgc tggtgcagat ggtatgcaat ccgttttttt cgattatttg ttgcacttcc
    43561 gaaaaagttg cttcgctatg taatttggtc cttatccaat cgggttttcg ctgaaagttc
    43621 atgtttgttg ggatgaattg taaaaattat tctttttctt tttgcgattc ttcaaaaaat
    43681 tgccaaagtt tgtcgtcttg gggcgcagga gcaacaattt ttatccaatt tttcgagacg
    43741 ggatgaacga attcaatttt tcgggcgtgc aaactgatgc ctccattgga attgggacgc
    43801 ggaaaaccgt atttcaaatc gccacgaata ggacagccga tttttgccaa ctgacagcga
    43861 atttgatgat gtcgcccggt ttcaagttga atttcaagca aataataata gtcggaactt
    43921 gtcaacacct tgtagtgaag aatggctttt tttgcattat ctaccggccg atcaaaagca
    43981 gtggttttgt tcttagcttc atttctcacc aagaaatgtt ccagcgtatc gaccattttg
    44041 ggcggttttt gctgaacaat tgcccagtat gtttttttta tttctttatt ttgaaacatt
    44101 tcattgagtc tggacaatgc tttatctgtt tttgcaaaca atacgactcc gctaaccggt
    44161 cggtcgaggc ggtgagttac tcccaaaaaa acgtttcccg gtttgttgta tttgtttttc
    44221 agatattttt tcagcgtttc cgatagaggt tcatctccgg tcttgtctcc ctgaacaatt
    44281 tctgaaacgg tcttatttac ggcaataatg tgattgtctt cgtacaaaat ttccatgcaa
    44341 aaatgaagct ttttgaagca aatattttgt taacagctgg aaaaattttt attggttttt
    44401 ttgctctcgt tgtctgatga cttcgtaaat caaaatacct gccgcaaccg aaacgttgag
    44461 tgatgaaatg ttcccgaaaa tcggaatttg aactttttcg ttgcaaagtt gcaataattc
    44521 gggactgatg ccttcatctt ccgaacctaa aacgatggca gtgggttcgt taaaatcaat
    44581 tgccgtataa tttttttctc ccttttcggt ggcagctatc aatttaatac ccgaatcagc
    44641 aaggaattga agtgtttcgt acagattgtt ttcgcgacat accggaatcg aaagcaaagc
    44701 accggcagaa gtttttacag cgtcggaagt aatggaaact ccacctttgg caggaatcac
    44761 aatagcgtcc acgccggcgc actcacatgt tcgcgcgata gcaccaaagt tgcgtacatc
    44821 ggttattccg tccaaaacta ctataaaagg cattttcccg acttcgtaca cggcaggaat
    44881 aatatcttcg atgcgctgat aggttacggg cgaagtaaaa gcaataacac cctgatgatt
    44941 tttgtcggta aatttattta atttttccaa tggaacgaat tgcaccggaa tatgaagtcc
    45001 tttggtagcg gcgaaaagtt cacgtgaaat ttcattgctc atgtcgcgtc gcaacaaaat
    45061 tttgtcaatg tctttccccg actgtatggc ctccagtacc ggtctaatgc caaaaatcat
    45121 ttctttttcg ggtcgtcttt tgtttttgta gatagtcatg caattaattt ttacaatttc
    45181 tcgttacaga agtttttgtt tgatttcgcc aatacgcatt tctctttcaa aagaaaaagt
    45241 tttatccttt tcgtttagca aacgatacag ttccaatgtt ttttcgtaaa aaccattggc
    45301 ttcggcagac tgatgatttt gttccaaagc attggcagtt tcgtaggtca attgaaccag
    45361 caaatccaaa atggaagccg aatagtttgc tttgcgaaca gtttcgatga atttttctgt
    45421 cggttcaatt gataattctt tcaaatcaaa atttaaaagt tcgcgatagg tactgtcagc
    45481 cattttcaat gcatcttcgg gaaatccgct gtcgcgaaat cccaacatgg ctgcaatgac
    45541 gcggctcaat ttttcgagat atttcaataa ataatcttct gtgggcattt ttgaagtaat
    45601 cagttttgaa aaacaataaa atccaaaatt ttttgcaaag ttagccaaat aattgcattt
    45661 cttttttatg cgaacaaaaa taattatgtt atttcgttga caagtaaaaa gataggagca
    45721 tatttacaaa ttatgaacga cctgagtttt tttgaattat ctttgcaaaa attttttgca
    45781 aaaaatattg tttaacccaa tcataaattt aaaaaaaagt gttttcccat ataggagaaa
    45841 tagcaggact aatggtagcg ctctgttgga caatgagtgc tttgtttttt gagaaagccg
    45901 gtcataaaat cggttcactt acggtgaacg tgattcgttt atcgttggca gtggtatttc
    45961 tgggtatcac tacttttttt acacgcggct ttttctttcc cactgatgct acacattatc
    46021 aatggttctg gcttggactt tcgggtatta taggtttctt tttgggcgat ttgttccttt
    46081 tcaaatccta tatggtgatt ggttcgcgta cagcacagtt gattatgagc ttgtgtccca
    46141 tgttgacggc tttaatcggt tggttttttt tggatgaaaa gctttcgttg aaaagtattc
    46201 ttggcatagt gatatgtgta acagggatta tcatagccat ttcaaatcgg aatttgaagc
    46261 tgaatattcc ttttaaaggt tttttactgg cttttggagg tgctttggga caggcggtag
    46321 gattaatttt aagcaaaaaa ggaattggaa attacgatcc tgttgcagct actcaaatac
    46381 gagccctgtt tggactagtt tgttttgctt tatttatcac ttttttgggt cgatggaagc
    46441 aagtctcagc cgcttttaca aacaaatccg cattaaaatc ggtaacggta ggcacatttt
    46501 tcggcccgtt tgtaggcgtg gcgttatcca tgtttgccat tcaacataca aaaaccggca
    46561 tagcttccac attaatggca ctggttccgg tgtttatcat ttggccttcg gcagttatgt
    46621 ttaaagaaag aatcaccatt ccacagataa tcggagcatt tgtcagcata gcaggcgtca
    46681 gcttgttttt cctttgagta gaataaaaaa aggttctcga aaattatcgg agaacctctt
    46741 ttcctgtttt ttaaaaaaat tgcttaaatg atgccctgag ccatcatggc ttttgcaact
    46801 ttcatgaatc cggcaatgtt tgcacctttc acataattta catatccgtc ggcttcagtt
    46861 ccgtatttta cacaagcttc gtgaatattt tgcataattt gctgaagctt ttgatccact
    46921 tcttctcttg tccaattcaa tctcatggaa ttttgagtca tttccaatcc tgatacggaa
    46981 actccaccgg cattggccgc tttacccgga gcataaagaa ttttattttt caggaacaca
    47041 tcaacggctt caggagttga aggcatattg gctccttcgg aaacggcaat acaaccattt
    47101 ttaacaagca tttcggcatc ttcgcctgtt atttcgtttt gcgtagccga aggcaaagcg
    47161 atagtacatt tttcgctcca gggacgtgca ccttcaacat atttgcagcc atatttttcg
    47221 gcatattctt taatacgtcc acgataaaca tttttcagtt cgaaaacgta agccagtttt
    47281 tcttcatcga tgccatccgg gtcataaata taaccgttgc tgtcggaaaa agtaataact
    47341 ttggctccga gttggagtgc tttctgggca gtgtaggttg caacgtttcc tgaaccggaa
    47401 atggcaacca cttgaccttt cagatcgtga ataccttttg ttttcagcat attcatcaag
    47461 aagtagatgt tcccgtatcc ggtggcttcg ggacgaatca gtgaacctcc aaattcgaga
    47521 cctttccctg taagaacacc gttggcttca tttctgagtt ttttgtacat gccgtacata
    47581 taaccaattt cgcgtccgcc aacgcctatg tcgccggcag gtacatcggt atcaggaccg
    47641 acaattttgt tcaattcaag cataaatgcc tggcaaaaac gcattacttc agcgtccgat
    47701 tttcctttgg gattaaaatc ggaaccacct ttagctccac ccattggtag agtagttaag
    47761 gcgtttttaa aagtttgttc aaaagcaaga aatttcaaaa ttccgggata tacggaagga
    47821 tggaaacgca aaccaccttt gtaaggtcca atggcgttgt tgtgctgcac tctgtaaccc
    47881 atgttggttt gaatgttgcc cttgtcgtca acccaagtta cgcggaaaga taaaattctg
    47941 tccggaataa ccaagcgttc taccaaattt tgtttttcaa attcgggatg agcattgtaa
    48001 acatctacaa tagattccaa tacttcctgt actgcctgat aatactccgg ctcattggga
    48061 aatctccttt tcaaattgtc aattacctgt tctactttca ttttgtttat gttttttatt
    48121 ttttatagtt ttcggttgca aagttataac aaaatagtaa tatctgtata tcaaaaagcg
    48181 gaaattttgc aatatttttt attatttttc ttatgatgac tgttgacatt tttatttttt
    48241 tcggaagaaa tttatttgta ttgttgttaa atttaaaatt atcctatcaa aacgacagta
    48301 tttatatttt atgaaaatga tttttttggt tttgtccgag atttttcttt aatattagaa
    48361 aaaaaactgt atttttgcaa cataaaattt tgttgaccgg aaagttgccg gagtggtcga
    48421 tcgggacggt ctcgaaaacc gttgtacggg taa
//
LOCUS       DAOTAX010000008        46510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_644_length_46510_cov_28.778151, whole genome shotgun sequence.
ACCESSION   DAOTAX010000008 DAOTAX010000000
VERSION     DAOTAX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 46510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46510
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_46510_cov_28.778151"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(619..876)
                     /locus_tag="PLM04_01830"
     CDS             complement(619..876)
                     /locus_tag="PLM04_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01853.1"
                     /translation="MNEKQTKNAAESIVKALVELSLGKEPNIFSKSPFRKLAEHKNYT
                     LIRDAYIDYLKEFDGKIDSEEDMKRLFDFRIKILNYFNDDK"
     gene            complement(889..2637)
                     /gene="aspS"
                     /locus_tag="PLM04_01835"
     CDS             complement(889..2637)
                     /gene="aspS"
                     /locus_tag="PLM04_01835"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HON01854.1"
                     /translation="MYRTHNCGELRISHVGQIITLAGWVQRIRKMGGMVFIDLRDRYG
                     ITQLVFNQEINSDLWEKANKLGREFVVQVTGKVSERSNKNPNLSTGDIEILVSELTVL
                     NESKTPPFTIEDATDGGDDLRMKYRYLDLRRNVVRKNLELRHQMTIETRQYLDNLGFL
                     EVETPVLIASTPEGARDFVVPSRMNPGEFYALPQSPQLFKQLLMVGGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVEQEDVLQTFEGLVKHLFKTIKNIDFPEPFPRMEWS
                     EAMKRFGSDKPDIRFGMELVELKEMVTGHDFSVFDNAEYVGGICAPACASYTRKQIDE
                     LTEFVKRPQIGAKGLIYIRFENDGSFKSSIDKFFSVDDLKKIASLFSANPGDLILIMS
                     GEKLKTQKALCELRLEMGGRLGLRKKDEFAPLWVIDFPLFEWNEELNRYQAMHHPFTS
                     PKPEDIPLLDTQPGEVRANAYDMVVNGVELGGGSIRIHNSNLQNKMFELLGFTPEKAQ
                     AQFGFLMNAFQFGAPPHGGLAFGLDRFVSLLAGLDSIRDCIAFPKNNAGRDVMINAPS
                     PLDPSQLEELHLMLKL"
     gene            2872..3306
                     /gene="ruvX"
                     /locus_tag="PLM04_01840"
     CDS             2872..3306
                     /gene="ruvX"
                     /locus_tag="PLM04_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HON01855.1"
                     /translation="MGRILAIDYGQKRVGIAVSDVMHISANGLTTLPVNEVFPFLEKY
                     LVQENVETIVVGFPRQMNGKESDSMKFIRPFVAKLVQKFPQIELIYEDERFTSVLAHK
                     TMLEGGLKKKGRQNKALVDKISATIILQSYLESVRYKNERNI"
     gene            3334..3885
                     /gene="def"
                     /locus_tag="PLM04_01845"
     CDS             3334..3885
                     /gene="def"
                     /locus_tag="PLM04_01845"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HON01856.1"
                     /translation="MILPIYTYGNPVLRKVAQPIDASYPELKSLVNNMFETMYHADGV
                     GLAAPQVGLPIRVLVIDLAPFKDEDAELGSFKIAMINPTILERSAELLPGEEGCLSIP
                     GIHETVFRSKTIKVHYFDTDFNEYEEIFEDYKARVIQHEYDHLEGNLFTDRVNPLRRQ
                     LLKSKLTNISKGKIQCNYKIITA"
     gene            complement(3912..5216)
                     /locus_tag="PLM04_01850"
     CDS             complement(3912..5216)
                     /locus_tag="PLM04_01850"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HON01857.1"
                     /translation="MNYEQTLDYLYSRLPVFHQIGSAAYKPGLENTIRILTALHQPQN
                     SFRSIHVAGTNGKGSVSHFLSSILQSSGYKTGLYTSPHLVDFGERIRVNGEMIDKQFV
                     IDFVENHKKLIEEVQPSFFEITMAMAFDYFASSKVDVAVIEVGLGGRLDSTNIIQPEL
                     SVITNISFDHEEFLGHTLQEIATEKAGIIKPHTPVVIGEALPETKPVFLQKAQEMNAP
                     IFFSENIKQIFFDRYEENRMIVKTSDGKSYTVGLTGNYQLKNIATVLTAVDQLRKANF
                     EIPEASLQEGLKKVIEKTGLQGRWQIISASPKIIADTGHNPGGIAFVAEQLKTQHYQT
                     LRIIFGMANDKDIDTVLSLLPKNGVYYFTQANIKRAFPSDQLLKRGQQAGLYGKSFSN
                     IEEAIKAALDEANKEDLIFIGGSNYVVGEALTFFEKHEEVTQ"
     gene            complement(5271..5813)
                     /locus_tag="PLM04_01855"
     CDS             complement(5271..5813)
                     /locus_tag="PLM04_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON01858.1"
                     /translation="MESFLDLVKRRQSAHSFTNKPVEKEKIDRILEIVRLSPSACNAQ
                     PWKFIVVTDEEKRQEIADATSKRFLPVNHFSKQAPVHIVIVEESTNFFTKVGCYGVNR
                     QYPLIDIGIATAHLCLAATNEGLDTHVIGWYNEKKIQQALEIPENKKVMLVVALGYSD
                     EEVRPKKRKKMEELVSFNKY"
     gene            5966..7294
                     /gene="tilS"
                     /locus_tag="PLM04_01860"
     CDS             5966..7294
                     /gene="tilS"
                     /locus_tag="PLM04_01860"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HON01859.1"
                     /translation="MILKKVELYIEKNKLLFPEKGKILVGVSGGRDSVALLDILIKLG
                     YQCTVAHCNFHLRGEESDRDEKFVQQLSFNLNIPYYLVDFDTVNYAKQKNISIEMAAR
                     ELRYSWFTSLAEKINAQAIAIAHHADDNVETLLMHLVRGTGLKGLTGISPKNGLIVRP
                     LLCCSRNEINEYIKNNNLSFIEDSTNQSVDFQRNKIRLQVIPLLEEINPSVKKVLSES
                     IERFSEINTFYENAIEKIKKQLLTVDNDQLKINIDLLCKQASPKTILFEILHPYGFNE
                     SIVQDIEKHLHDESGKIFYSPTHYLIKDRKYLIISNKIKKNETTFFITENDSEISFPL
                     HLSITRKKKEKNFVISKDNRIIQIDASLIQYPLTLRRWTYGDTFFPFGMNRQKKLSDF
                     FIDQKLNLKQKAETWLLLSQNQIVWVVGLRLDNRFKITESTQEVLEIKML"
     gene            7523..9385
                     /gene="rho"
                     /locus_tag="PLM04_01865"
     CDS             7523..9385
                     /gene="rho"
                     /locus_tag="PLM04_01865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HON01860.1"
                     /translation="MSNYNILQLNAKELSELKQIASELGLKVPGTITKDKLVYEILDQ
                     QAIANAQQKVASNEKKENERKKRMRVSVKKNVEQKVFSSPNPTITPAPSNSTTSPTPL
                     VPFTENSPKQEIQNNQTSDNTIEAPASTTEVKPKKSGRQSKHNKTSKSAPKTLLKKTS
                     ADTPEKPEVNIPLEEIDNFEVPNLPEVEITEEQDIDIEKDTQIPVQIIETSEPRPGLS
                     LNINKNANGNNNENKVKIAPRQDKTEKQFDFDGILEGTGTLEIIQDGYGFLRSPDYNY
                     LSSPDDIYVSQSQIKLFGLKTGDTVTGTIRPPKEGEKYFPLIKVLKINGRSPEYVRDR
                     VSFEHLTPLFPNEKFKLTTGVNDPLSVRIVDLFSPIGKGQRGLIVAQPKTGKTVLLKD
                     IANAIAANHPEVYMIVLLIDERPEEVTDMARSVKAEVISSTFDEPAERHVKVASMVHE
                     KAKRMVECGQDVVILLDSITRLARAYNTVAPASGKILSGGVDANALHKPKRFFGAARN
                     IENGGSLTIIATALTETGSKMDDVIFEEFKGTGNMELQLDRKLANKRIFPAVDILASS
                     TRREDLLLDQDTLNRMWILRRHLSDMNSLEAMEFLKERMERTVNNEEFLVSMNA"
     assembly_gap    9611..9710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9711..10453
                     /locus_tag="PLM04_01870"
     CDS             <9711..10453
                     /locus_tag="PLM04_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HON01861.1"
                     /translation="TMHIGDIDTLYAQSQYEGNIVPSLSWSSSDSSVVSVDKNGKISA
                     QKKGNAIITASAGDKSATCNVTVDDEIVISFTKAELVFWGDYYQTGNSNNYSLYLTNT
                     QDTLLIEINTDTLSTKGIPSGTYEMIENFTPFDQIVPSTLLPAFVYGDYSYGSFLFNA
                     VAVYPIYSGLATFSNEGNIYTISYNFLDYFGNHIAGEYSGTIIFYDETENPSAVASNV
                     KAIKMNKKIHQFSSNRKISKVLKLINAR"
     gene            10566..11312
                     /gene="fabG"
                     /locus_tag="PLM04_01875"
     CDS             10566..11312
                     /gene="fabG"
                     /locus_tag="PLM04_01875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HON01862.1"
                     /translation="MKLLEGKVAIVTGAARGIGKSIAIKLAQEGADIAFTDLVIDENA
                     SNTQKEIESLGVKVKAYASNAANFEETHAVVEEIQKEFGHIDILVNNAGITRDGLLIR
                     MTESQWDSVINVNLKSAFNFIHACAPIMMKQRNGSIINMSSVVGVSGNAGQANYSASK
                     AGLIGLTKSVAKEFGSRGIRANAIAPGFIETEMTHALTEEQRAKWVENIPLRRGGTPE
                     EVAKVTLFLASDLSSYVSGQVIHVCGGMNT"
     gene            11524..14826
                     /gene="secA"
                     /locus_tag="PLM04_01880"
     CDS             11524..14826
                     /gene="secA"
                     /locus_tag="PLM04_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HON01863.1"
                     /translation="MALNNFFKKILGNKAQRDLREIEPIVEKIQAVYPEIKSLSNDDL
                     RARTASLKERIQEYVAPEVNRIQELKNSIEETEIDLREKIYTEIDKLEKEITDKQEKI
                     LNEILPEAFNIMKETARRFAQNDEIIVKANNFDRNLAAKHDFVYIDGEEAHYKNEWMA
                     GGSMIKWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALTGNGVHIVT
                     VNDYLSKRDSEWMGPLFMFHGLSVDCIDKYRPNSEERRQAYLADITYGTNNEFGFDYL
                     RDNMATSPLDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPVPKGEDQLFEEFRPTVE
                     RLVNAQKVLATKYLTDARNLLKSENEKEREEGALALFRSYKGLPKNKALIKFLSEPGN
                     KTILLKTEEYYMQENNRNMHIATDPLYFVIDEKNNSIELTDKGFDLLSETSEDPTFFV
                     LPDVGSEIAELEKNHTLTPEEKQAQKDEILQNYSIKSERIHTVNQLLKAYTLFEKDVE
                     YVVINNEVKIVDEQTGRIMEGRRYSDGLHQALEAKERVTVEAATQTFATITLQNYFRM
                     YHKLAGMTGTAETEAGEFWDIYKLDVVVIPTNRPVIREDMEDRVYKTKREKYAAVIDE
                     IVRLMEAGRPVLVGTTSVEVSELLSRMLTGRKIKHNVLNAKQHQREAEIVAEAGRKGT
                     ITIATNMAGRGTDIKLTPEVREAGGLAIVGTERHESRRVDRQLRGRAGRQGDPGSSVF
                     YVSLEDDLMRLFGSERISSVMDRLGFQEGEMIQHPMISRSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYTRRRHALMGERIESDITNMIYDAIENMVANAKSNGFYEELEE
                     QLLTVFAMDVPFSEEEFNSARTRDLTDKVVNAALTTFKRKMEKLSSIAYPVIKQVYEQ
                     QGDKYENILIPVTDGKRIFNVVVHLETAYKTEGKEVIKSFEKQTLLYVIDDEWKEHLR
                     QLDELRNSVQNATYEQKDPLLIYKLESFGLFKEMIENINHKVLSILMRGQIPVREPEK
                     VREAKIPARQDFSKYKTQKDEIAERGNSNAGDTREKQRPQPVHVEKKVGRNDPCPCGS
                     GKKYKHCHGANA"
     gene            14858..15748
                     /gene="lgt"
                     /locus_tag="PLM04_01885"
     CDS             14858..15748
                     /gene="lgt"
                     /locus_tag="PLM04_01885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HON01864.1"
                     /translation="MLNYIVWNVNPEIFRLGSLSVRWYGLLWAVGILLALLIGQKIFK
                     HEKLPEVWLDKIFIYAVIGGIVGARLGHCLFYEWKLLPEPVQFLGITFKYGNYYLSHP
                     WEILYVWHGGLASHGGAIGILIAMYFYNKNVTKKGYLWAFDRLAIGVAITGACIRLGN
                     LMNSEIYGNPTNLPWGFIFVRAGETQPMHPTQIYEMLYCLITFGVIWWLYWKKEAYKK
                     TGFIFGVFLIGIFGTRFLLEFIKLDQEAFESGMLLNMGQILSIPFIVWGIWLVARKQK
                     ITPTSDVNANKNKPVANRKK"
     gene            complement(15763..16779)
                     /gene="holA"
                     /locus_tag="PLM04_01890"
     CDS             complement(15763..16779)
                     /gene="holA"
                     /locus_tag="PLM04_01890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HON01865.1"
                     /translation="MIKQEAAADEILQEIKSKNFHSVYFLMGEEPYYIDKISNYIQEN
                     MLSESEKDFDLTILYGKDVEISTVINTAKRFPMIAPYQVVIVKEAQMISSWENLVYYL
                     QKPLKSTILVFCYKYGTPDKRTKWFQETKKAGVVFESGKLYDYQVGEWIRSYLKTRKT
                     GITQQAENMLVDYLGTDLTKIANELDKLLLTKPQDTQQITPELIERNIGISKDYNVFE
                     LQKAFVNRDILKANRIILYFAENKKNNPLVLVLAQLFNFFGNLLLFHYMKDKTEKNIA
                     SELKINPFFVKDYQNAAKIYDREKTIKVLSLIRETDARSKGVDNAGIDEGELLKELVY
                     KILH"
     gene            complement(16823..17596)
                     /locus_tag="PLM04_01895"
     CDS             complement(16823..17596)
                     /locus_tag="PLM04_01895"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HON01866.1"
                     /translation="MKTKKEIVENWLPRYTKRPLEEFTPYILLVNFNIYVELFAGYYQ
                     VPIVGKDGNMPNASANGITIINFGLGSPNAALIMDILSAIQPKAVLFLGKCGGLRDKN
                     KEGDYILPIAAIRGEGTSNDYFPPEIPSLPAFSLQRSVSSNIRDIGKDYWTGTVYTTN
                     RRVWEHDEKFKEYLRMTRAMAIDMETATLFSSGFYNGIPTGALLLVSDMPMYESGIKT
                     AESDKRVTANFVEEHLRIGIESLTSIMHNSRTIKHLRFE"
     gene            17680..18138
                     /locus_tag="PLM04_01900"
     CDS             17680..18138
                     /locus_tag="PLM04_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HON01867.1"
                     /translation="MLQLNLPSYNFKIKKVNETLYIFDSQRKKYVKLTPEEWVRQNFI
                     RFLLEEKHFPSAYLAVEKELVFNGMKKRCDAVLYDGQAQPLMILEFKAPHVPLTQSVF
                     DQVAVYNYRLNVEYFIVSNGLEHYCCRVMKEHTKFEFLKEIPDYTNFLNQ"
     gene            complement(18168..19079)
                     /locus_tag="PLM04_01905"
     CDS             complement(18168..19079)
                     /locus_tag="PLM04_01905"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HON01868.1"
                     /translation="MVDLKVDIGKLHLKNPVMTASGTFGYGMEYSDFIDISRIGGIIV
                     KGTTLRKREGNPYPRMKETASGMLNAVGLQNKGVDHFIEHIYPQLTNVDTNIIVNVSG
                     STIEEYVETAEKLNNLEKIPAIELNISCPNVKEGGMLFGTSCTAAKEVVAKVRRAYKN
                     VLIVKLSPNVTNISEIAQAVEEAGADAISLINTVLGMAIDAETRKPCLSTVTGGLSGP
                     AIKPLALRMVWETARDVKIPVIGIGGIMNAADAIEFMLAGASAVQVGTANFIDPTVTI
                     KIIEGIEDYLNRHGYTSVSDIVGALET"
     gene            complement(19073..19843)
                     /locus_tag="PLM04_01910"
     CDS             complement(19073..19843)
                     /locus_tag="PLM04_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HON01869.1"
                     /translation="MKKLILDLILKKNIRLNEQVYLLIFSSEKGLPEILPGQFAQVKI
                     NDSPATFLRRPFSVNFVDYENNELWLLIQVVGAGTLKLSETSPGASVNMILPLGNSFS
                     FPEKKDEKILLIGGGSGIAPLLFFGSWLFDAGFSPEFLIGARSSIHLVEVEEFKKYGN
                     VYITTEDGTEGEKGFVTDHSILKKRNYDRIYSCGPTPMMKAVGAYAENNNIFCEVSLE
                     NTMGCGIGACLCCVTDTTQGRVCVCTEGTVFNIEKLKW"
     gene            complement(19949..20434)
                     /locus_tag="PLM04_01915"
     CDS             complement(19949..20434)
                     /locus_tag="PLM04_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON01870.1"
                     /translation="MDLKDRIEKIMQNEAMNAVQFASEIGIQGSTLSHILNGRNKPSL
                     EVVMKILKRFDYINSDWLIFGEGPMSRTERQSKTPTLFDSQDLSNTNTVNYTQKTEQK
                     IESLQSHFQQKPEQKEIISESVNSKIPEENKIAPIVIEKIIKRIIVYFNDNTFQEFTT
                     T"
     gene            complement(21365..22141)
                     /locus_tag="PLM04_01920"
     CDS             complement(21365..22141)
                     /locus_tag="PLM04_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HON01871.1"
                     /translation="MATEPKTEKEISVEEKLRALYELQRVYSKIDEIRNLRDELPLEV
                     QDLEDEIIGLNTRLENYKNEIAELEAAIKAKKLEIEQARTKIEKYKEQQENVRNNREY
                     DNLSKEIEYQTLEIELCEKRIKEYTAALEAKKAEQQSTMEALSERRLDLEQKKGELNE
                     IISETKQEEERLREQIKQIEQRVEPRLLTAFKRIRKNSRNGLSVVPVQRDACGGCFNK
                     IPAQRQLDIRSRKKIIVCEYCGRILVDPELAGIVPPTSES"
     gene            complement(22147..23240)
                     /locus_tag="PLM04_01925"
                     /pseudo
     CDS             complement(22147..23240)
                     /locus_tag="PLM04_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
     gene            complement(23255..>23561)
                     /locus_tag="PLM04_01930"
     CDS             complement(23255..>23561)
                     /locus_tag="PLM04_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01872.1"
                     /translation="IFTENGEIPLSEVFEKIKVKENGAKCAVDAKSGNEKLVQYLQEI
                     LPDYDKNRVYPSDIRKLINWYNILVDAKLTDFAASETETGDKVETVSDKTEEISSEK"
     assembly_gap    23562..23661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23662..23868)
                     /locus_tag="PLM04_01935"
     CDS             complement(<23662..23868)
                     /locus_tag="PLM04_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HON01873.1"
                     /translation="MLKEILYVSGKPGLYKMVSQGKNLMVIESLSDGKRIPVFSSDKL
                     VYLKDIAIFTENGEIPLSEVFEKIK"
     gene            complement(23913..24632)
                     /gene="hisA"
                     /locus_tag="PLM04_01940"
     CDS             complement(23913..24632)
                     /gene="hisA"
                     /locus_tag="PLM04_01940"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HON01874.1"
                     /translation="MEIIPAIDIIDGKCVRLSQGNYDKVTLYSRYPDEIAKMFADEGF
                     RRLHLVDLDGAKAQHIVNYKVLEKITSTTDLVVDFGGGLKSDDDLRIAFECGASMVTG
                     GSIAVKNPEIFVGWLEKYGGEKIILGADVKNEKIAVSGWTESTELELIPFVHNYFSKG
                     VKYVICTDISRDGMLQGPAIELYKKIISAEPQVHLIASGGVGSLHDIEMLNEAGIPAV
                     IVGKAIYEGNISLQELSRFLF"
     gene            complement(24785..25372)
                     /locus_tag="PLM04_01945"
     CDS             complement(24785..25372)
                     /locus_tag="PLM04_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01875.1"
                     /translation="MVHEFGKSLHYEKSQFRRADAFYREVLLVDEIIRFATDSKEDMK
                     MQLQDIDVQLIKTNRVCHVSEKFREKDFVDLYIEVFSKYPHTRGWLETGLADMLVYFV
                     PEVVYWCNAKNLRKFCVDMLFPMIPAEWYKEIYCSATSIISKNMNFFDQQTIVHLIKA
                     NNQTSDGNRWCTIGISVPFEFLKNNGLNIRKYLFP"
     gene            complement(25384..26670)
                     /locus_tag="PLM04_01950"
     CDS             complement(25384..26670)
                     /locus_tag="PLM04_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HON01876.1"
                     /translation="MKNLCFYFEIHQPMRLKRYRFFDIEQDHYYYDDFQNEERIRMLA
                     EQSFLPANRTLLEIIQNSNGKFRCAFSFSGIVLEQLEQYAPEVIDSFEELSKTGCVEF
                     MAETYAHSLSSVYDIHEFEQQVALQTEKIESLFGKKPTVFRNSELIYSDEIGEKIYNM
                     GYRTILIDEVSHVLGWKSPNFVYRHPTLPKLKLLVRNFKLSDDIAFHFSDRSWSDYPL
                     TADKFISWIKNMPDDQPVVNIWLGYEALGLFQRPESGIFEFLKAIPFFALQNNIGFLM
                     PSEVAKKIEAVDILSSPEPISWSGIEKDISLWNGNDLQQEALEKLYAVGERVRLCTDR
                     PLRHDWLMLQSSDHFRYMSHKDAFGTYYESPYDAFMNYMNILADFLLRVDEQYPTTID
                     NEELNALLNTIYELEKEVERLENELKKLRSRKKTEN"
     gene            complement(26852..28108)
                     /locus_tag="PLM04_01955"
     CDS             complement(26852..28108)
                     /locus_tag="PLM04_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HON01877.1"
                     /translation="MKVLMFGWEFPPHILGGLGTASYGLVKAMAHQPDMEITFVIPKL
                     YGDEDQSFLKIIGACYVPVVWKENDWNYVNERIGWIMNPDEYFWLRNGIKYDFRRIYT
                     NDLGCIEFSGRYPDNLLEEISNYEAVASVLAHQLSFDVIHSHDWLTYPAGIFAKQISG
                     KPLVVHVHATEFDRSRGNVNPIVYDIEKRGMDAANHIITVSNLTRNIVIEKYFQDPKK
                     VTTVHNAVEPLPDVDSYVKTPRSDKIVTFLGRITMQKGPEYFVEAANRVLKKNKKVRF
                     VMAGNGDMMQDMINLAAKRGITDRFHFTGFLKGRQVYQMLADSDVYIMPSVSEPFGIS
                     PLEAMQVGTPTIISKQSGCSEILTHSIKIDYWDIDGMADAVNAIIRNPAMYKSLHELG
                     REEVNNIRWEDAGKKVIKIYNSLLGK"
     assembly_gap    28130..28229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28331..30271)
                     /locus_tag="PLM04_01960"
     CDS             complement(28331..30271)
                     /locus_tag="PLM04_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HON01878.1"
                     /translation="MSYLSFDKSLLINLERSLSKEILRTNRAGTYNSSTLIDCNTRKY
                     HGQLVVPIPELGEANHVLLSSLDETIVQHGIEFNLGVHQYEGNNFYPNGHKYIRQFDC
                     EIIPRTIYRVGGVILSKERMLVSFEPRVLFKYTLLEAHSPTLLRFKPFLAFRNVHELT
                     HQNPEANCDFQPVSNGIAMKLYENYPTLFMQLSKKNVYQHFPDWYKNIEYYKERERGF
                     EYREDLLVPGSFEIPIKKGESVIFSAGISEVSTSSLKKLWDKELEKRNPRCDMFSALK
                     NSAQQFYKREGDKTYLLAGYPWFTARARDEFVALPGCTLSIGRMDYFDAIIETAIPEI
                     ESFINENKVSSKLKEIDAPDVLLWFVWTIQQYARHTSLSETAQRFGKLVASIISFVRK
                     QRHPNLFLHNNGLLYVNGLEKPATWMNAVENGRPITPRTGYVVEINALWYNALKFSSQ
                     LLGEMGEEMEANVIDYQSGILRESFIKTFWNGNYLYDFVNGNYNDKEVRPNMIYAVGL
                     PFSPLEKSQQKLILDIVTKELLTPKGLRTISPKSGMYRPVYIGGQLERDRNYHNGPVW
                     PFTFGAFVEAYLKIYKMSGKSFIERMMIGFEAEMKELCVGTLSELYDGNPPFKGHGGM
                     SFAMNVGEVLRVMEILKEYKED"
     gene            30661..31344
                     /locus_tag="PLM04_01965"
     CDS             30661..31344
                     /locus_tag="PLM04_01965"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HON01879.1"
                     /translation="MTSNIESERVLEKQRWKFAIILPSIVCILWILCFVLERGMGWDF
                     HKAGIYPRKLENIWSIFTYSFIHADWKHLMNNVLSFFILGICLYYFYSEVANRVLIIS
                     AVLSGLILWCIGRENFHIGASGIIYSLAFFLFFSGIIRKHIPLIAISLIVVFIYGSMV
                     WHLFPWLPDDPVSWEGHLSGAVTGIVLAFVYRHRGPQPPKPEWEEDEELDEEYEWEEN
                     YDQEQWKEE"
     gene            31381..32343
                     /locus_tag="PLM04_01970"
     CDS             31381..32343
                     /locus_tag="PLM04_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01880.1"
                     /translation="MDEKNTKEINLLDLIKMFNAWVVKVGKSFINFILYLLKAGYRKK
                     YLTLTLIVLCFAIGEYMARPSNRQYAADAMASLYGVDAFTMKEIAKQLEQSRANNQYT
                     SLGTKLLLPDSIAKNIVSFNSYFVIDYLNNSTPDMVDFANNHSLEDTLNVRMKDKIYF
                     RLVTRNISQVPQVEKAILNFFNSQPTVRLLYTARQNELKGKIDLSSAEMQRLDSLAKI
                     SYFKDNDQQLKWSKDRLIVGEQQKQLFYGDMLLLQKVKAEAESELLTYKQPVELPSGF
                     VVNPFPMNGRLKYGVYGILIGLGLGLALAVCLEQLQKVVQFFRK"
     assembly_gap    32354..32453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33176..34651
                     /locus_tag="PLM04_01975"
     CDS             33176..34651
                     /locus_tag="PLM04_01975"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HON01881.1"
                     /translation="MKQKFLSILFLFVNYSFLISAQNNLSTNDTGEVQTIKVIDLDSL
                     LKNTKWIQNNETNSEIIELSEDSDTLKTKHLEKIKVLKDSFQLFVSPTALATIKSDSL
                     LYLSNPFFIELVYMGLPFDFDIKPKEDLLTLFHERKASTLEENAYRPIKVPTSQESLI
                     KLRQDARNEITRTAVGLYKRMFYELSNPYVKGNRPIQVKPIEKVEFVDKNKYVNGKER
                     KMHVPEFQVGPWRHKGSFLLQFSENYISKNWYQGGNSNLSVLGILSGQINYDDKKRTQ
                     WENSLEWRSGFYSVFGDTTALRTLNTNEDVLKINSKFGYKASGNWFYSGAIDFSTQFL
                     KNFKSVNSNELKASLFTPIRLNVNVGMDYKYKKIFSLMVSPLSLKYIYLSDTVKVNPN
                     LFGIQKGKNHLEQIGSSFKAVVSYAPSREIQLDSKFAFYTNYEKIEVDWEIVCNMIVN
                     RFVSTRISINPRYDNTMIMAEGERAKVQFKQLLSVGFSHKF"
     gene            complement(34661..35197)
                     /locus_tag="PLM04_01980"
     CDS             complement(34661..35197)
                     /locus_tag="PLM04_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HON01882.1"
                     /translation="MHPFSIIEKYYVKDSPLYRVLVIHSQQVRDKALEVAKQHPELNI
                     DTEFVGEACLLHDIGIFLCHAPRIYCFGEHEYIEHGYLGSDLLKKEGLPQHALVAERH
                     TGTGFTKEEIRLKKLPLPDRDMLPLSLEEQLICYADKFYSKSKLNLQLSVEEIRQNLS
                     FYGKEKVEKFDSWHQLFG"
     gene            complement(35348..36589)
                     /locus_tag="PLM04_01985"
     CDS             complement(35348..36589)
                     /locus_tag="PLM04_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HON01883.1"
                     /translation="MEGMKNFRYKIGFLALLLISLFPISIFAQSSGSENLVIESLQKF
                     WNNTGLANFEFKYLAMIVVGLVFIYLGIAHKWEPLLLVPIGFGILIGNVPFTDGFRIG
                     IYEQGSVMNILYSGVVQGWYPPLIFLGIGAMTDFSSLISNPKLMMLGAAAQLGIFVSF
                     IIAYAVGFSPAQSGAIGIIGGADGPTAIFISSRLAPELMGAIAVAAYSYMALVPVIQP
                     PIMKLLTTNKERKIRMKPPRQVTKLEKIIFPIIGLLLTTFISPSAMPLLGMLFLGNLL
                     KESGVTERLADTARNALLNSITILLGVTVGASTQADRFLTVDSLMIFGLGAFSFMIAT
                     AGGVLFAKIMNLFLKEGNKINPLIGSAGVSAVPDSARVSQDVGLSYDKSNHLLMHAMA
                     PNVAGVIGSAVAAGILLGILS"
     gene            complement(36591..37037)
                     /locus_tag="PLM04_01990"
     CDS             complement(36591..37037)
                     /locus_tag="PLM04_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HON01884.1"
                     /translation="MKKFDFNINGNDFHVYIKSIDGNIAEIEVNGTPYTVTLNQEVKV
                     SKTPILVRKEVPTTPSNMNVADKFNPVPSNQKPSSTVIKSPLPGNILRINVKEGDTFK
                     EGDVLLVMESMKMENNILAERNGKIVKVCVSVGKAVLQDEVLFEIE"
     gene            complement(37037..37384)
                     /locus_tag="PLM04_01995"
     CDS             complement(37037..37384)
                     /locus_tag="PLM04_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HON01885.1"
                     /translation="MIMKLLMINSWEELLTAAAIGIMVVFSVLFVLVLLFELFQVIVL
                     KVAKKKVVKSGIDVDKIKDIPANEIAAISMALYLYLDSMHDEESNVLTIKRIERRYSP
                     WNSKIYGLNNLHR"
     gene            complement(37388..38947)
                     /locus_tag="PLM04_02000"
     CDS             complement(37388..38947)
                     /locus_tag="PLM04_02000"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HON01886.1"
                     /translation="MNNLEKLEQLISLRAKAKLGGGEKKIESQHKKGKYTARERIEML
                     LDDGSFEELDMFVTHRCTNFGMENEKYLGDGVVTGHGTIDGRTVYVYAQDFTVFGGSL
                     SEMMAQKICKVMDMAMKMGAPIIGINDSGGARIQEGVTSLAGYAEIFERNILASGLVP
                     QISAIFGPCAGGAVYSPALTDFILMTEENSYMFVTGPKVVKSVTGEDITTEDLGGANV
                     HSSKSGVSHFKVESEEEGLLMIRKLLSYLPQNNLEEPPLIQNYDPIDRHEDKLNTIVP
                     DNPNLPYDMKEIIHSLVDNGEFLEVHQFYARNIIVGFARLNGASIGIVANQPNFLAGV
                     LDCDASRKAARFVRFCDAFNIPILTLVDVPGFLPGSGQEYSGIIIHGAKLMFAYGEAT
                     VPKVTVTIRKSYGGAHDVMSCKQLRGDFNYAWPTAEIAVMGASGAIEILEGKNIANIA
                     DPEEKAKYIAEKVAEYNEKFSNPYQAASYGYIDDVIEPRNTRFRIIRAFETLQTKKVV
                     NPLKKHCNIPL"
     gene            complement(38984..39400)
                     /gene="mce"
                     /locus_tag="PLM04_02005"
     CDS             complement(38984..39400)
                     /gene="mce"
                     /locus_tag="PLM04_02005"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HON01887.1"
                     /translation="MKPKKIEHIGIAVKSLETAIPFFESVLGLTCYAIEEVKDQKVKT
                     AFFKVGDTKIELLESTDPEGPIGKFIEKRGEGVHHIALAVENVQQALNEASDKGVMLI
                     DKQPRKGAEGLNIAFIHPKSTFGILTELCENPNINC"
     gene            40066..40758
                     /locus_tag="PLM04_02010"
     CDS             40066..40758
                     /locus_tag="PLM04_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HON01888.1"
                     /translation="MKKLFLLSIMMVFTFFSFQSGQNKKGVEKSQNTLPMDLKTFDTI
                     QLLAPDTMGGKSLMQTFQHRKSDREFKQTPLSLKHLSEILWAAYGINRPDGKRTVPSA
                     RALYPLQVYAVLPNGIYRYEPQTHRLVPVVKGDYRKLAGLQDFVYTAPMNLIFIADFN
                     KYNLTQKEPDANSIRMVSLDAGHCTQNVYLYCASEGLRCVVRAGAKADELLSLLSLDS
                     HYRFIVAQTVGY"
     gene            complement(40788..42113)
                     /locus_tag="PLM04_02015"
     CDS             complement(40788..42113)
                     /locus_tag="PLM04_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HON01889.1"
                     /translation="MNWKQLITTKRLGMEAYHEKRQDNRSEFQRDYDRLIFSPPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVSSVGRSLGINVARQIKEKNNLPDAFIDEVGSIV
                     SAACLAHDMGNPPFGHSGEKAIATYFSEGEGQILQEKMLPEEWSDLISFEGNANTFRV
                     LTHQFRGRRPGGFVLTYSTLASIVKYPYASVFSDPQRQKFGFFHSEKSDYEKIAHELG
                     IPHSFSQPDKYVRYPLVYLVEAADDICYQIMDIEDAHKLKILSTDETKALFLSYFEGK
                     KLERKLQTIAMVDDVNEQIAYLRSSVIGQLIDNCSSVFLENEPQILEGSFSSTLIKLL
                     PPVSLKAYQNCSKTAFEKIYCSSEVLDVELAGYKIIFTLLENLVKAVLSPEKAYSQQL
                     LMRIPKQYEIDQPTTYGKVMAILDYISGMTDVYALDLYRKITGMNIPTL"
     gene            complement(42165..42899)
                     /locus_tag="PLM04_02020"
     CDS             complement(42165..42899)
                     /locus_tag="PLM04_02020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HON01890.1"
                     /translation="MNTSSSQRKISDPELGDIYFNRSQRATRINIQILEDKLKVTLPM
                     RASEKDAMRFIDSVREKILKKRAQVKEKIRKNKQIIDEQSPFRALTFVTDIKPTHRED
                     IYCCLKNQVLSIEIPSEVDCREEQMQNYLHRIIARYLRLEAKRVLPQRTRLLADKFGF
                     RFSTVKIQSSRTRWGSCSAKGNINLSLYLMALPAHLIDYVILHELCHTRELNHSTDFW
                     RWLNRVTDNQSDRLKEELKNFNFNWD"
     gene            complement(43081..44304)
                     /locus_tag="PLM04_02025"
     CDS             complement(43081..44304)
                     /locus_tag="PLM04_02025"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HON01891.1"
                     /translation="MAYINENFIKIPPTYLFSEIAKRVEQHKAENPSAKIIRMGIGDV
                     TLPLPDASVDAMIKAAEEQRHAETFRGYGPEQGYEFLRQAIVENDFRARGIKLDVDEV
                     FVSDGAKSDTGNIGDIFGIDNCVAITDPSYPVYVDTNAMAGRAGEPNEFNEWNGLTYL
                     PCNEANNFVPQLPDKKVDMIYLCYPNNPTGTTLSKQQLQVWVEYAKANRSVILFDAAY
                     EAFITEENVPHSIYEIEGAKEVAIEFRSFSKTAGFTGTRCGYTVVPKELVGYTHQGEA
                     VALNKLWLRRQSTKFNGTSYIVQRGAEATFTPEGRKQVKQLIEYYLGNARIIREGVAK
                     LGLKVFGGVNAPYVWVKAPDNMKSWEYFDFLLKQKNIVTTPGAGFGRSGEGYIRFSAF
                     GSRENTIEAMERLIK"
     gene            complement(44383..45216)
                     /gene="dapF"
                     /locus_tag="PLM04_02030"
     CDS             complement(44383..45216)
                     /gene="dapF"
                     /locus_tag="PLM04_02030"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HON01892.1"
                     /translation="MKFTKMHGIGNDYIYINGFEEQLENPGELSIRLSDRHKGVGSDG
                     LVIILPSSKADFRMQMFNADGSEAEMCGNAARCIGKYVYEKGMTDKTELTLETLAGIK
                     TLQLFLDERNNVVSVTVDMGKPVLEAEKIPTTLPGKQIVNFPVSFDGINYAITCVSMG
                     NPHTVIFTENIANMDIEKMGKKIENAPIFPRRTNVEFIEVIDKSRIEMRVWERGSGET
                     MACGTGACASVVAGVLNGLVSRKATVELLGGNLEIEWNENDEHVYLTGPAETVFEGEI
                     S"
     gene            complement(45407..46432)
                     /locus_tag="PLM04_02035"
     CDS             complement(45407..46432)
                     /locus_tag="PLM04_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HON01893.1"
                     /translation="MKCFASDNYSGIHPDILKAIVEANADHEISYGDDTYTQRAKKLF
                     QQKLGNVEVLFVFNGTGANIVCLKCCTLPFQSIVCAQTAHILTDECGAVFQNIGCSIM
                     PVHTPDGKLTPELISPVLVHKGNVHSAQPKVISISQTTEVGTLYDTEELKALCDFAHK
                     NNMLVHVDGARISNAVAALGKSLKEVTVDCGVDIMSFGGTKNGMMMGEAILIFNDELN
                     ANARFYQKQSLQLYSKNRFIAAQFMALLKNDLWLKMATHSNKMASLLAEKMRAIPEVQ
                     ITLPVQANAVFAIIPEKVIEPLREKFRFYTWDFQTNELRWMCSFDTKPEEIDEFAETL
                     KKLLADR"
BASE COUNT        14790 a         8785 c         8022 g        14513 t
ORIGIN      
        1 cagaagcaat tccaaaggca aatataactt atttttaatt caaaactcat ttttttttat
       61 taaaaaggcg aatttgaata aaattcaatt aaaatgttat tataattaac tgaatatcaa
      121 tatgttaaat agaatgtcaa aaataggaga ggagaagtga aataatgtct tattaaaaat
      181 cgtttattga gctattgaaa atcaattaat tatgaaaccc acatgtattg tatatacaaa
      241 cacgaataaa tataatattc agacatgtgg gttccataag acctttaata aataaattat
      301 attttgatga aatttctatt taatttctat ttatttatga atcaattaag tagtttcaaa
      361 cgatattttt caaaaaataa ttttgcaaat aacttagaaa caatttgaac aaaattttat
      421 tgatcattca tttaaaaaac tctgttttgc tgcaattttg atatgatatt tgttagtatg
      481 gaatattatt tgaaatcaca ctattgtaaa ctatttcaga ataaaataaa tttgcttaat
      541 tttcctataa tttatattat gtcaaataag aattgatgaa attctttctt catactctat
      601 cctaaaactg aaatattttt atttatcgtc attgaaataa ttcaaaattt ttatgcggaa
      661 atcgaaaaga cgcttcatgt cttcttccga atcaattttg ccatcgaatt ctttcaaata
      721 atcgatataa gcatctctga ttaaagtata atttttatgt tcggccaatt ttctaaatgg
      781 tgatttggaa aaaatatttg gttcttttcc caaactaagt tcaaccaaag ccttaacaat
      841 cgattcggca gcatttttgg tttgtttttc attcatggca aatgtttttt aaagtttgag
      901 catcaaatgt aattcttcca gttgcgaagg atccaatggt gacggagcat ttatcattac
      961 atctcttcca gcattatttt ttggaaaagc aatacagtcc cttatcgaat caagtccggc
     1021 caataaagaa acaaaacggt cgaggccaaa agccaaacct ccatgcggag gcgctccaaa
     1081 ctgaaaagca ttcatcagaa aaccaaattg agcttgagct ttttccggtg taaatcccag
     1141 taattcaaac attttattct gcaaattgct gttatgaatt cggatagagc ccccacccaa
     1201 ttcaactcca ttcactacca tatcgtaggc attggctcta acttcaccgg gttgggtatc
     1261 caacaaaggg atatcttcag gttttggaga tgtaaatggg tgatgcatag cttgatagcg
     1321 atttaattcc tcattccatt caaacaatgg aaaatcgata acccaaagag gtgcaaattc
     1381 gtcttttttg cgaaggccta accttcctcc catttccaat cgtagttcgc aaagtgcttt
     1441 ttgagttttt aatttttctc cggacatgat aagaattaaa tcgcccggat tggcggaaaa
     1501 taacgaagca atttttttca aatcatcaac agaaaagaac ttgtcgatag aagatttgaa
     1561 ggaaccgtca ttttcaaagc gaatataaat cagtcccttt gctcctattt gaggtcgttt
     1621 tacaaattcc gtcaactcat caatctgttt gcgtgtatag gaagcgcaag ccggagcaca
     1681 tattccacca acatattcgg cattgtcgaa aactgaaaaa tcgtgtcccg ttaccatttc
     1741 tttaagttcg acaagttcca ttccaaagcg aatatccggt ttatcggaac caaaacgttt
     1801 cattgcttcg ctccattcca tacgtggaaa tggctctgga aaatctatat ttttaatggt
     1861 tttgaacaaa tgctttacca atccttcaaa agtttggagc acatcttcct gttccacaaa
     1921 agacatttca caatcaatct gtgtaaattc gggttgacga tcggcacgca aatcttcatc
     1981 acggaaacat ttgacaattt ggaaatagcg atcgaatccg ccaaccatta aaagttgctt
     2041 gaacaattgg ggagattgag gtagcgcata aaactctccg ggattcattc tggatggaac
     2101 tacaaaatcg cgtgcacctt caggtgtaga agcaatcagc acaggagttt ccacttcgag
     2161 aaaacctaaa ttgtccaaat attgacgtgt ttcaatggtc atttgatgtc gaagttccaa
     2221 attttttcta acgacatttc tccgcaaatc gagataccga tacttcattc gcaaatcatc
     2281 gccaccatcc gtagcatctt caatagtaaa aggaggtgtt tttgattcat tcaaaacggt
     2341 gagttcggaa accaaaattt ctatatcgcc cgtacttaaa ttgggatttt tattgcttct
     2401 ttcagaaact ttaccggtaa cctgtaccac aaattcgcga cccagtttgt tggctttttc
     2461 ccacaaatcg gaattaattt cctgattgaa aaccaattga gttataccat agcgatcgcg
     2521 aagatcaata aataccatcc ctcccatttt acggattctc tgtacccagc cggctaaagt
     2581 aataatttgt ccaacatgag atatgcggag ttcaccacag ttatgagtcc tatacattta
     2641 ttattctgat aattaataaa ttaataatac ttttattttt aaagctacaa acgaaaagaa
     2701 aattatcttt atcattcaac aaaaatacaa aaaatccgtg tacctaaacc aatagacaca
     2761 aaaattcatt ttatacgaca attaaaaata attttagcca taggttgtga gtaagttgaa
     2821 aatttttaaa atgaacacta ttttgtaact ttgcattttc aaatgaagaa aatgggaaga
     2881 atactggcaa tagattatgg gcaaaaaaga gttggaattg cagtaagcga tgtgatgcat
     2941 atttctgcaa atggactgac aacattgccg gtaaatgaag tttttccttt tcttgaaaaa
     3001 tatttggtgc aagaaaatgt tgaaaccatt gttgtaggtt ttccacgaca aatgaacggc
     3061 aaagaatctg attccatgaa atttatccgg ccatttgttg caaaattggt tcagaaattt
     3121 cctcaaattg aacttattta cgaagatgaa cgttttacat cggttttggc tcacaaaacc
     3181 atgctcgaag gcggtttaaa gaaaaagggc agacaaaaca aagcgttggt cgataaaatt
     3241 tctgcaacaa ttattttgca aagttatttg gaatcagtta gatacaaaaa tgaaagaaac
     3301 atataacact cgaattacaa taaaaaacaa ataatgattc ttcctattta cacatacgga
     3361 aatcccgttt tacgaaaagt tgcacaacca attgatgctt catatcccga attaaaatct
     3421 ttggtaaaca acatgtttga aaccatgtat catgctgacg gtgtcgggtt ggcagcgcca
     3481 caagttggtt tacccataag agttttggtt attgatttgg ctccatttaa agatgaagat
     3541 gcagaattgg gcagttttaa aattgccatg attaatccca ctattctcga acgcagtgct
     3601 gaattgttgc cgggcgaaga aggttgttta agcattcccg ggattcatga aacggtattt
     3661 cgttccaaaa cgataaaagt ccattatttc gataccgact tcaacgaata cgaagagatt
     3721 tttgaagatt ataaagcacg cgttattcaa cacgaatacg accatttgga aggtaacctg
     3781 ttcaccgacc gagtaaatcc actccgacgt cagttgctca aatcaaaact taccaatatt
     3841 tcaaaaggta aaattcagtg caattataaa attattactg cttgaaaatg atcgaataat
     3901 tgattttgaa tttattgagt cacttcttcg tgcttctcga aaaacgttaa agcttctcca
     3961 acaacataat tgctgccgcc gataaagatt aaatcttctt tatttgcctc atccaaagca
     4021 gcctttatag cttcttcgat atttgaaaaa gattttccat aaagccctgc ttgttgtcct
     4081 cttttcaata attggtcgga agggaatgcc cttttaatgt tggcttgtgt gaaataatag
     4141 actccgtttt tcggcagcaa tgaaagtacc gtatcgatgt ctttatcgtt tgccattccg
     4201 aaaattattc ttagtgtttg ataatgttgc gtttttaact gttccgcaac aaaggcaatt
     4261 ccaccgggat tgtggcctgt atcggcaata attttaggtg atgcagaaat aatttgccat
     4321 cttccctgaa gtcctgtttt ttcgataact tttttcaaac cttcttgtaa agaggcttcc
     4381 ggaatttcaa aattagcttt gcgcagctga tcaacagcag tcaaaaccgt tgcaatattt
     4441 ttaagttgat agttgcctgt caatccaact gtataagatt ttccatcaga agttttcact
     4501 atcatccgat tttcttcata tcgatcaaaa aaaatctgtt tgatgttttc tgaaaaaaag
     4561 atgggagcat tcatttcctg tgctttctgt aaaaaaacgg gtttcgtttc cggcaaagct
     4621 tctccaataa ccaccggtgt atgcggtttt atgatgccgg ctttttctgt agcaatttcc
     4681 tgcaaagtat gtcctaaaaa ttcttcgtga tcaaaactga tattggtaat taccgaaagc
     4741 tcaggctgaa taatgttcgt actgtcgagc ctgcctccca aaccaacttc tattactgca
     4801 acatcaactt tgctgctggc aaaataatcg aaagccattg ccatagtaat ttcaaaaaac
     4861 gaaggctgca cttcttcaat caatttttta tgattttcca caaaatctat aacgaattgt
     4921 ttatcaatca tttcgccgtt cacacgaatt ctttcgccaa aatcaaccaa atgaggcgaa
     4981 gtgtacaggc cggttttata acctgaagac tgcaaaatgg aagacaaaaa atgtgatacg
     5041 gaaccttttc cgttcgttcc ggcaacatga atagaacgaa acgaattttg cggttgatgc
     5101 agggctgtaa gaatacggat tgtattttct aatcccggtt tatatgcagc actgccaatt
     5161 tgatgaaata caggtaaacg cgaataaaga taatctaatg tttgttcgta attcattaat
     5221 ttacaaataa atattcttat ttagattttc acaaaaataa gaattttcaa tcaatattta
     5281 ttaaaggaaa ccaattcttc cattttcttt ctttttttgg gtcgaacttc ttcatcggaa
     5341 tatcccaaag caacaaccag cataactttt ttattttcag gaatttcaag agcttgctga
     5401 attttttttt cattatacca acctatcaca tgcgtatcca aaccttcatt cgttgcagcc
     5461 aaacataaat gagcagtggc aatgccaata tcaatcagtg gatattgacg atttacgcca
     5521 taacagccaa ccttcgtaaa aaaattggtg ctttcttcaa caattacaat atgaaccgga
     5581 gcttgtttgg aaaaatgatt gacaggaaga aatctttttg aggtagcatc agcaatttct
     5641 tgtcgttttt cttcgtctgt aactacaata aatttccacg gctgcgcatt gcaagccgat
     5701 ggagaaaggc gcactatttc aagaatccgg tcgatttttt ctttctcaac cggcttgttg
     5761 gtaaaagaat gagcactctg acgcctttta accaaatcta aaaatgattc cataatacat
     5821 atttttttaa attgatcggc acttttgttg caatgacacg aaaaaaacta tttgctggaa
     5881 attatatatc tttgtaaatc tttgaattcg agaaaaattt tttataagtt tctatatttg
     5941 gcaaagataa tgtaaataaa agaaaatgat actcaaaaaa gtagaattat atatcgaaaa
     6001 aaataaatta cttttccccg aaaaaggaaa aattttagtt ggtgtgagtg gtggtagaga
     6061 ttcagtagca ttactcgata ttttaattaa attgggttat cagtgcaccg ttgctcattg
     6121 taattttcat ttgcgcggag aagaatccga tagagacgaa aaatttgtgc aacagttatc
     6181 gttcaactta aatattcctt attatttggt tgattttgat acagttaatt atgcaaaaca
     6241 aaaaaatatt tccatagaaa tggctgcgcg cgaattgcgt tattcgtggt ttacatcatt
     6301 agcagaaaaa atcaatgccc aagccatagc cattgctcac catgccgatg ataacgttga
     6361 aacattatta atgcatcttg ttcgtggcac cggattgaaa ggcctgacgg gaatttctcc
     6421 caaaaacggt ctgattgttc gtccgttgct ttgttgcagt agaaatgaaa ttaacgaata
     6481 catcaaaaac aacaatttaa gttttattga agattctaca aatcaatcag ttgattttca
     6541 aagaaataaa attcgacttc aagtaatccc acttttagaa gaaataaatc cgtcggtgaa
     6601 aaaagttctt tccgaatcca ttgaacgatt ttcggaaatc aatacctttt atgaaaatgc
     6661 catcgaaaaa ataaaaaaac aactgcttac cgtcgacaac gatcagctaa aaatcaatat
     6721 tgatttgctg tgcaaacaag cttctccaaa aactattctg tttgaaattt tacatcctta
     6781 cggcttcaac gaaagtattg ttcaagatat tgaaaagcat ttgcatgacg aatcgggcaa
     6841 aatattttat tctcctaccc attatttgat taaagacaga aaatatttga ttatcagcaa
     6901 taaaataaag aaaaatgaaa caactttttt cattacggaa aatgattccg aaatcagctt
     6961 tccccttcat ctttccataa caaggaaaaa aaaggaaaag aactttgtta tttcaaaaga
     7021 caatcgaatc atacaaatag atgcgtcctt gattcaatat ccacttacct tgcgcagatg
     7081 gacgtatggt gatacgtttt tcccttttgg tatgaaccga caaaaaaaat taagtgattt
     7141 ttttatcgat caaaagctga atttaaagca aaaagctgaa acatggctgt tgctttcgca
     7201 aaatcaaata gtttgggtag taggtcttcg actcgacaat cggtttaaaa ttaccgaatc
     7261 aacccaggaa gttcttgaaa ttaaaatgtt gtaaataagc gctcaacaaa ttatccttga
     7321 aaaatatttt aataaatttg tccatttaac acaaaatgat taattttgca gcgtaaaagc
     7381 aaatcgattt aaaaaagcat tcctgctttc attctctaca atcaaaaata tttttattgc
     7441 atttctacgc aaaaaatctt tttttattct gaattttatt ttgaatattt gaaaaatccc
     7501 aactttattt ttatccaatt ttatgagtaa ttataatatt ttgcaattaa atgcaaaaga
     7561 attatccgag ttaaaacaaa ttgcctccga actcggcctg aaggtacccg gtaccattac
     7621 aaaagacaaa ctggtttatg aaattcttga tcaacaagcc attgcaaatg cacagcaaaa
     7681 agttgcatca aatgaaaaaa aggaaaacga aaggaaaaaa cggatgaggg tttcggtaaa
     7741 gaaaaatgtt gaacaaaaag ttttttcttc accaaaccca actatcacac ccgcaccttc
     7801 caattccaca acttctccaa ctcctttagt tccctttact gaaaattctc ccaaacaaga
     7861 aatacagaac aatcagactt ctgataacac gattgaggct ccagcttcaa ctactgaagt
     7921 aaaacctaaa aaatcgggca gacaatcgaa acacaacaaa acttccaaat cagcccccaa
     7981 aacccttcta aaaaagactt ctgccgatac tccggaaaaa ccagaagtaa atattccttt
     8041 ggaagaaatt gataattttg aagtcccgaa tcttcccgaa gtcgaaatta ccgaagaaca
     8101 ggatattgat attgaaaaag atactcagat tcccgttcag attattgaaa cttccgaacc
     8161 acgtcccgga ctttctttaa atatcaacaa aaatgctaac gggaataata atgaaaataa
     8221 ggtaaaaatt gcacctcgac aagacaaaac cgaaaaacaa ttcgattttg acggaattct
     8281 ggaaggaacc ggaacacttg aaattattca ggatggctat ggatttctgc gttcgcccga
     8341 ttacaattat ctttcttccc ctgatgatat ttacgtttct caatctcaaa tcaaactttt
     8401 cggactgaag accggcgata cggttacagg caccattcgt cctcccaaag aaggggaaaa
     8461 atattttcct ttaataaaag ttctgaaaat caatggtcgt tcgcctgaat atgtgcgcga
     8521 tcgtgtttct tttgaacatt tgacacctct tttccccaac gaaaaattta aacttactac
     8581 cggcgttaac gatcctcttt ccgtgcggat tgtagatttg ttttctccta ttggtaaagg
     8641 tcaacgcgga ctaattgttg cacaacccaa aaccggaaaa acagttttgt tgaaagacat
     8701 tgccaatgcc attgccgcca atcatcccga agtttatatg attgttttgc tcatcgatga
     8761 gcgacccgaa gaagttactg acatggcacg cagcgtaaaa gccgaagtca tttcatctac
     8821 tttcgacgag ccggctgaac gccacgtaaa agtggcaagc atggtacacg aaaaggcaaa
     8881 acgcatggta gaatgtggac aagatgtggt cattttgctc gattccatta cccgtttggc
     8941 tcgtgcttac aatacagtgg ccccggcttc cggaaaaata ctttccggtg gcgtggatgc
     9001 caatgctctt cacaagccaa aacgattttt tggagctgca agaaatatag aaaacggtgg
     9061 aagcctcacc ataattgcga cagcccttac cgaaacaggt tcaaaaatgg acgatgttat
     9121 ttttgaagaa tttaaaggta ccggcaatat ggaattgcaa ctcgaccgga aattggccaa
     9181 caaaagaatt ttccccgctg tggatattct ggcttccagc actcgacgcg aagatttact
     9241 gctcgatcag gatactttga acagaatgtg gattttgcgt cgtcatcttt ccgatatgaa
     9301 ctctttggaa gcaatggaat ttttgaagga acgcatggaa cgtaccgtaa acaacgaaga
     9361 atttctcgtt tcaatgaatg cttgatttat cataacttgt aattcaatat cttatttcta
     9421 taaccaataa ttttgtaata aaaatgaata aattatttag attgggtatc gtctcttttc
     9481 ttatcgtttc cctgttcaca tcttgtgaaa aggaaaataa tcaggttacc tctgtaacta
     9541 ttagcgaaac cagcaaaact atgcatattg gcgatatcga cacactctat gcccaatctc
     9601 aatacgaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaactatgca
     9721 tattggcgat atcgacacac tctatgccca atctcaatac gaaggcaata ttgttccatc
     9781 tttgtcttgg agttcaagcg attcatcagt agtttcagtg gataaaaacg gaaaaatttc
     9841 ggctcaaaaa aagggaaatg ccattattac ggcttctgca ggcgataaat cggctacctg
     9901 caacgttacg gtagatgatg aaatcgtaat ttcatttaca aaagcagaat tggtattttg
     9961 gggtgattat tatcaaacag gcaattccaa taattactct ttgtatctta ccaatacaca
    10021 agatactctg ctgattgaaa tcaacaccga tactctttct accaaaggaa ttccgtcagg
    10081 aacttacgaa atgattgaaa attttacccc ttttgatcaa atagttcctt caactttact
    10141 tccggcattc gtatatggtg attattctta tggttcattt cttttcaatg ctgttgcagt
    10201 ttatcctatt tattcgggac ttgcaacttt cagcaatgaa ggaaacattt atacaatttc
    10261 ctacaacttt ttggattatt ttggaaatca tatagccggt gaatattcag gaacaattat
    10321 tttttatgat gagacagaaa atccatcagc agtagcttca aacgtgaaag ctataaaaat
    10381 gaacaaaaaa attcatcagt tttcatcaaa ccgtaaaatt tcgaaagtat tgaaattaat
    10441 caatgcaaga taattttata caatcatatt taataatttt cgttttgaac aatcgtttca
    10501 agaatcgtta ataaatttta caatactcga aaaaacaaaa ttaattattt caaaaacaat
    10561 tttttatgaa attattagaa ggaaaagtag ctatcgtaac cggagctgca cgcggaatag
    10621 gcaaatccat tgccatcaaa ttggctcaag aaggagcaga tattgctttt accgatttgg
    10681 ttatagacga aaatgcgtca aatacgcaaa aagaaattga atctcttggt gtaaaagtga
    10741 aagcttatgc gtccaatgct gccaattttg aagaaacgca tgctgttgta gaagaaattc
    10801 aaaaagaatt tggccatatc gacattttgg tcaataacgc cggtatcacc agagacggtt
    10861 tattgattcg tatgacagaa tctcaatggg attcggttat taatgtaaat ctcaaatctg
    10921 cttttaattt tattcatgcc tgcgctccca taatgatgaa acaaagaaat ggcagtatta
    10981 tcaacatgag ttctgtagta ggcgtttcag gaaatgcagg acaagccaat tattctgctt
    11041 ccaaagccgg attaatagga ttgacaaaat cggtcgcaaa agaatttggc tcacgcggta
    11101 ttcgtgccaa tgccattgct ccgggattca tcgaaacaga aatgactcat gctttgactg
    11161 aagagcagcg tgccaagtgg gtagaaaata ttcctttacg tcgtggtgga actcccgaag
    11221 aagttgccaa agttacactt ttccttgcat ctgacttgtc gagttatgtt agtggacagg
    11281 ttattcatgt ttgtggtggc atgaatactt aaaaccaaat atccttttat atatttcttc
    11341 aaacgcttct gacaacggaa gcgtttttgc tttttatcaa ctataagaag aatttttata
    11401 cattatccat ctggttttaa ctttaaaaaa atgtatcttt gcaaaacttt ttgcttacag
    11461 atatgtttaa gcacgagtca ttttatttat tcatcagaga gcgtataaaa aatttacaaa
    11521 ataatggcat taaataattt ttttaaaaaa atactgggaa acaaagcaca acgtgactta
    11581 agagaaattg agcccatcgt tgaaaaaatt caagctgttt atcctgaaat caaatctctt
    11641 tcaaatgacg atctcagagc aagaacagct tccttgaaag aacgtattca agaatacgtc
    11701 gctccggaag ttaatcggat tcaagaatta aaaaacagca tagaagaaac ggaaatcgac
    11761 cttcgagaaa aaatttatac cgaaatagat aagctcgaaa aggaaataac cgacaagcag
    11821 gaaaaaattc tcaacgaaat tcttccggaa gctttcaata taatgaaaga aactgctcgc
    11881 cgttttgccc aaaacgatga aattattgta aaagctaaca attttgatcg taatcttgca
    11941 gccaaacatg attttgtata cattgacggt gaagaagccc attacaaaaa tgaatggatg
    12001 gcgggtggca gcatgattaa atgggacatg gttcactacg acgttcagct tattggtggc
    12061 gtggtattgc atcagggaaa aattgccgaa atggccacag gtgagggaaa aacactggta
    12121 gctactttgc ccgttttttt aaatgctctg accggtaatg gtgttcatat cgttacggtg
    12181 aacgattatt tgtccaaacg cgactccgaa tggatggggc cactatttat gtttcatgga
    12241 ttatctgtag actgcatcga taaatatcgt ccaaattccg aagaacgccg tcaggcttac
    12301 ctcgctgaca taacctacgg aaccaacaat gaattcgggt ttgattactt gcgcgataac
    12361 atggcaacga gccctttgga tttggtacaa cgtaaacacc attatgccat tgtcgacgaa
    12421 gtggactctg ttttgattga tgatgcaaga acacctttga ttatttccgg ccctgtaccc
    12481 aaaggagaag atcagctttt cgaagaattt cgccctactg tagagcgttt ggtaaatgca
    12541 caaaaagtgt tggctaccaa gtatcttacc gatgcccgta acttgctgaa atcggaaaat
    12601 gaaaaggaac gtgaagaagg tgctttggct cttttccgct cctacaaagg tttgccaaaa
    12661 aataaggctt tgattaaatt tttgagtgaa ccgggaaata aaaccatttt gctgaaaacg
    12721 gaagaatact atatgcagga aaacaatcgg aatatgcaca tagctaccga tcccctgtat
    12781 tttgtaattg atgaaaaaaa caattctatt gaacttaccg ataaaggatt tgatttactt
    12841 tcggaaactt ctgaagatcc cacatttttc gttttaccgg atgtcggaag cgaaattgcc
    12901 gaactggaaa aaaatcatac gctgacaccc gaagaaaaac aggctcaaaa agatgaaatt
    12961 ctgcaaaact attccattaa atcagaacgc attcacaccg tcaatcaatt actgaaagct
    13021 tatactttat ttgaaaaaga tgttgaatat gtggttatca acaatgaagt taaaatagtt
    13081 gatgaacaaa ccggacgtat catggaagga cggcgatatt ctgacggact gcatcaggca
    13141 ctcgaagcca aagaacgcgt taccgtagaa gctgccacac agacttttgc caccattaca
    13201 ctgcaaaatt acttcagaat gtatcacaaa cttgccggta tgacgggaac tgccgaaact
    13261 gaagccggag aattttggga catttataaa cttgatgtgg tggttattcc taccaatcgt
    13321 cctgttattc gcgaagatat ggaagaccgc gtttacaaaa caaaacgtga aaaatatgct
    13381 gctgttatag atgaaattgt tcggctgatg gaagccggta gaccggtatt ggttgggaca
    13441 acatctgtag aagtatccga attattaagc cgaatgctca ccggtagaaa aataaaacac
    13501 aacgtgttaa atgccaaaca gcatcaaaga gaagccgaaa ttgttgctga agccggacgc
    13561 aagggaacaa taaccattgc taccaatatg gcaggtcgtg gtacggatat taaacttact
    13621 cctgaagtta gggaagccgg aggattggcc atcgttggta cggaacgaca tgaaagccgt
    13681 cgtgtggatc gtcagttgag aggacgtgcc ggtcgtcagg gtgatccggg ctcttccgtt
    13741 ttttacgttt cgttggaaga tgatttgatg cgtctgtttg gttctgaacg tatttcgagc
    13801 gtcatggacc gacttggctt tcaggaagga gaaatgattc agcaccctat gatttcccgt
    13861 tccatcgaaa gagcacaaaa gaaagtagaa gaaaataact ttggcatccg taaacgatta
    13921 ctcgaatacg atgatgtgat gaattctcag cgcgaagtta tttatacccg tcgtcgacat
    13981 gctttaatgg gagaaagaat agaatcggat atcaccaaca tgatttatga tgccattgaa
    14041 aatatggtgg ctaatgccaa aagcaacgga ttctatgaag aacttgaaga acaactgttg
    14101 acggtttttg ctatggatgt tcctttttcc gaagaagaat tcaattctgc ccgaacacgc
    14161 gatttgactg acaaagtggt taacgcagca cttactacct tcaagcgaaa aatggaaaaa
    14221 ttgtcttcca tagcctaccc cgtgattaaa caggtatatg aacaacaagg agataaatat
    14281 gaaaacatcc tgattcctgt aactgatgga aaaaggattt ttaatgttgt tgttcatttg
    14341 gagacagctt acaaaacaga aggcaaagaa gtaatcaaat cttttgaaaa acagacttta
    14401 ctttatgtga ttgacgacga atggaaagaa catctgcgcc agttggacga actgcgaaat
    14461 tcggttcaaa acgcaactta cgaacaaaaa gatcctctgt tgatatataa actcgaatcg
    14521 ttcggattgt tcaaagaaat gattgaaaat atcaaccaca aggttctctc cattttgatg
    14581 cgtggacaaa ttcctgtaag agaaccggaa aaagtcagag aggcaaaaat acctgcacgt
    14641 caggatttca gcaaatataa aacgcaaaag gacgaaattg ctgaaagagg aaattcaaat
    14701 gccggcgata ccagagaaaa acagcgtccg caacctgtcc atgtggaaaa gaaagtggga
    14761 agaaatgatc cctgtccgtg cggtagtgga aaaaaataca aacactgtca cggagcaaat
    14821 gcttaaaaat ttaatcccat aaaagaatcg aatcggcatg ctcaattata tagtttggaa
    14881 cgttaatccg gaaatatttc gtttaggttc attgtctgta cgatggtacg gattactttg
    14941 ggctgtgggt attttgctgg ctttactgat aggtcagaaa atttttaaac atgaaaagct
    15001 tccagaagtt tggctcgaca aaatttttat ttatgcagta attggcggta ttgttggggc
    15061 acgtttgggg cactgtctgt tttatgaatg gaaattactc cccgaaccgg tacaattttt
    15121 aggtattact ttcaaatacg gaaattatta tttgtcgcat ccctgggaaa ttctatatgt
    15181 gtggcatggg gggctggcaa gtcatggcgg agcaattggc attctgatag ccatgtattt
    15241 ttacaataaa aatgttacca aaaaaggata tctttgggca tttgaccgtt tagctattgg
    15301 tgttgccatt accggtgcgt gcataaggct cgggaatttg atgaattcgg aaatttatgg
    15361 aaatccgacc aatcttccgt ggggttttat ttttgttcga gcgggcgaaa cgcagcccat
    15421 gcatccaact caaatttatg agatgcttta ttgcctgata acatttggcg taatttggtg
    15481 gttgtactgg aaaaaagaag cctataaaaa aaccggattt atttttggcg tttttttgat
    15541 aggtatattc ggaacacgtt ttttactcga atttattaag cttgatcagg aagcttttga
    15601 atcgggtatg ttattgaata tggggcaaat tttaagcatt ccatttattg tttggggcat
    15661 ttggctggtg gcaagaaaac aaaaaattac gccaacatcc gatgtaaatg caaataaaaa
    15721 caagcctgtt gccaatagaa aaaaataatg ttataaactt gcttagtgca aaattttgta
    15781 aaccaattct tttaatagtt ctccttcgtc aattccggcg ttgtcgaccc ctttgcttct
    15841 tgcatctgtt tctctgatta gcgaaagtac ttttattgtc ttttctctat cataaatttt
    15901 agctgcgttc tggtaatctt ttacgaaaaa aggattgatt ttcagttcgg aggcaatatt
    15961 tttttcggtt ttatctttca tgtaatggaa caaaagcaaa ttcccaaaaa aattgaaaag
    16021 ctgcgccaat acaagtacca acggattatt ctttttattt tcggcaaaat atagaataat
    16081 tcggttggct ttcaaaatat ctcgattgac aaatgctttt tgaagttcga aaacattata
    16141 atccttgctg atgccaatat tcctttcaat taattccgga gttatttgtt gggtatcttg
    16201 tggcttagtt aaaagtaact tatccagctc attggcaatt tttgtcaaat ctgttcccaa
    16261 ataatcaacc agcatatttt cagcttgctg ggttattccc gttttacgag ttttaagata
    16321 cgatcgtatc cattcgccta cctgataatc atacagtttt cccgattcaa aaacaactcc
    16381 tgctttcttt gtttcctgaa accacttggt tcgtttatcg ggtgttccat atttgtaaca
    16441 aaaaaccaaa atagtcgatt ttaatggttt ttgaagataa tataccaaat tctcccatga
    16501 acttatcatt tgcgcttcct tcactattac tacctgatag ggagcaatca tgggaaatcg
    16561 tttcgccgta ttgatgaccg tcgaaatttc aacatctttt ccatagagaa tcgttaaatc
    16621 gaagtccttt tcactttctg aaagcatatt ctcctgtatg taatttgaaa ttttgtcgat
    16681 ataatacggt tcttcaccca tcaaaaaata aacggaatga aaatttttcg attttatttc
    16741 ctgcaaaatt tcatcagctg cggcttcctg ttttatcact ttaacaaaaa tattttatca
    16801 gttttataat ggcaactaat tgttattcaa atcttaaatg cttaatggtt ctgctgttgt
    16861 gcataatgga tgtaagcgat tctattccga ttcgtaaatg ttcttccaca aaattggccg
    16921 ttactctttt atcgctttct gccgttttaa tccccgattc gtacataggc atatccgaaa
    16981 ccagcagcaa tgcaccggta ggaatgccgt tgtaaaaacc ggaagaaaac aaagtggctg
    17041 tctccatatc gatagccata gctcgcgtca ttcgaagata ttccttaaat ttctcgtcat
    17101 gctcccatac tcttctgttg gtcgtgtaaa cagtgcctgt ccaataatct tttccaatgt
    17161 cgcgaatatt tgaagatacg gagcgctgta agctgaatgc aggcaaagag ggaatttcgg
    17221 gagggaaata atcatttgaa gttccttctc cacgaatagc cgcaataggc aaaatataat
    17281 ctccttcctt gtttttatct cttaatcctc cacatttccc caaaaataat actgctttgg
    17341 gttgaatggc cgacaaaata tccataatca aagctgcatt ggggcttccc aatccgaaat
    17401 tgattatggt aatgccattg gctgaggcat tgggcatatt tccatcttta ccgacaatag
    17461 gaacttggta gtaaccggca aaaagttcca cataaatgtt gaaattaacc agcaagatat
    17521 atggagtaaa ttcttcgagc ggtcgcttgg tatagcgggg aagccaattt tctacaattt
    17581 ctttttttgt tttcatagtt atttcagcat ttgttttgta acttcgtaag cttaaactgc
    17641 tccataaata aaaatttaat cgggacaaaa ttaggaaaaa tgttgcaatt aaatcttcct
    17701 tcttacaatt tcaaaataaa aaaggtgaat gaaacccttt atattttcga cagtcagaga
    17761 aaaaaatatg ttaagttgac ccctgaagaa tgggttcgtc aaaacttcat acgttttctt
    17821 ttggaagaaa agcattttcc ttctgcctat cttgctgttg agaaagaatt ggttttcaat
    17881 ggaatgaaaa aacgctgtga tgcagtttta tacgacgggc aagctcaacc tctaatgatt
    17941 ttggaattta aagctcccca cgttcccctt actcaaagtg tgttcgatca ggttgctgtt
    18001 tataattacc ggctaaatgt tgaatatttt atcgtaagta acggtttgga gcattactgt
    18061 tgccgggtga tgaaggagca tacaaaattt gaatttttga aggaaattcc cgattacacg
    18121 aattttttga atcaataaaa ttagaaaaaa gcttgttcta aaaactttca ggtttccaat
    18181 gctccaacaa tatctgaaac cgaagtataa ccatgacggt tgagataatc ttctattcct
    18241 tcaataattt ttatggttac cgtaggatca ataaaatttg ctgttccaac ttgaacggct
    18301 gaagctccgg caagcataaa ttctatggca tcggcagcat tcatgatacc acctatgcca
    18361 ataactggaa ttttaacgtc tcgagctgtt tcccaaacca ttctcaatgc caatggcttg
    18421 atggcaggtc cggataatcc ccccgtaacc gttgaaaggc aaggttttcg agtttcggca
    18481 tcaatagcca taccgagcac cgtattaatt agcgaaatag cgtctgcacc ggcttcttcg
    18541 acggcttgcg caatttcgga aatgttggta acatttggcg aaagcttaac aattaatacg
    18601 tttttatatg cccttcgcac tttagcaaca acctccttag ctgctgtaca tgaggttcca
    18661 aagagcattc cgccttcttt tacgtttgga caggaaatat tgagttcgat ggcgggtatt
    18721 ttttcgagat tattcaattt ttcggccgtt tctacatatt cttcaatggt agaacccgac
    18781 acattcacaa ttatatttgt atcgacattt gttaattgag gatagatatg ttcaataaaa
    18841 tgatcaacac ccttattttg cagtcccaca gcatttaaca tgcctgaagc agtttctttc
    18901 atgcgaggat aagggtttcc ttcacgtttg cgaagtgttg ttccttttac aataatacca
    18961 cctattcgtg atatatcaat aaagtctgaa tactccattc catagccgaa agttcctgat
    19021 gcagtcatta caggattttt caaatgtaat tttccaatgt caacttttaa atctaccatt
    19081 ttaatttttc aatattaaac accgttccct ctgtacaaac acaaactcga ccttgagtag
    19141 tgtcggttac acaacataaa catgctccaa tgccacaacc catagtattt tcaagtgaaa
    19201 cttcacaaaa gatattatta ttctcggcat aagcacctac agctttcatc atgggagtgg
    19261 gcccacaaga gtaaattctg tcataattcc tcttttttaa tatagaatga tctgttacaa
    19321 atcccttctc gccttcagtg ccatcttctg tagtaatata gacgtttcca tattttttaa
    19381 attcctccac ctccaccaaa tgaatactac tacgtgcgcc aataagaaat tctggggaaa
    19441 aaccggcatc gaaaagccaa gatccaaaaa aaagtaaagg agctatacca cttccaccac
    19501 ctatcagcaa aattttttca tctttttttt cgggaaaaga aaacgaattt ccaagcggta
    19561 aaatcatatt tacagaggct cctggagaag tttcagacag ttttaatgtt ccggctccaa
    19621 cgacttgaat cagcagccac aactcattat tttcgtaatc tacaaaattt acggagaaag
    19681 ggcgtcggag aaaagtagcc ggcgaatcat taattttaac ctgagcaaat tggccgggca
    19741 aaatttcggg aagtcctttt tcagaagaaa aaatcaacaa ataaacttgt tcgtttaagc
    19801 gaatattttt cttcaaaatt aaatcgagaa tcaatttttt catttagaat aagtaaagca
    19861 gaatgttata acattaaacc taaaaactcg gtcaatgata atgcaaaatt aaaagaaaaa
    19921 attaatccgt gaaaaacatc aacttttctc aagtagtagt aaattcctga aaagtattgt
    19981 cattaaaata cacaataatt cttttaatga ttttttcaat cactatagga gcaattttat
    20041 tctcttcagg aattttactg tttaccgact cggatataat ttccttctgc tcgggttttt
    20101 gctggaagtg cgattgaagt gattcaattt tttgctctgt tttttgagta taatttactg
    20161 tatttgtgtt agataaatct tgcgaatcga ataaagtagg cgttttagat tgccgttctg
    20221 tacgactcat tggcccttca ccgaaaataa gccagtcaga attaatgtaa tcgaatcgtt
    20281 tcaaaatttt catcaccact tccaagcttg gtttgttgcg tccattaagg atgtgcgaca
    20341 aagtggaacc ttgtattcca atttcggatg caaactgaac agcattcata gcttcatttt
    20401 gcataatttt ttcaattctg tcttttagat ccatgattca ataaaatttt aaagagaatt
    20461 gatgagattg attgaaatta caaagataaa tcattaattt cacaaaaaca aatttcatta
    20521 caaaagtaat atcaattttg taaattacaa aagtgaattt atcgatttac aaaaatgaaa
    20581 aatcagcgta taaaaatcag tcgtatttaa taaggctaca atacattaaa atggtttatt
    20641 aatggaatag tttaagttat atcgaaaaac aaagttatat atagtaaata attggtttac
    20701 aattaaatgg attatgaaaa catgtaaatt tcgatattat gacagaatat atttatggaa
    20761 gtaaaaattt tattaaagtc aaaatataga tattataata taattatctg gtttaatgta
    20821 aattattagt aatttttttg ttcaaaataa tttttacaaa tatgaatgta tagaattgtt
    20881 ttatataaat tattaagttt accaaagtga aaatatttat ttaaatcagg taattacatt
    20941 tgtaaatagg taaaatatat ttgtgaaata tacaaatgta tagattcctc aagttgaaaa
    21001 aagatgattt aaaaatttct ttgctatgat ctactcatgg atggatatag acactttgaa
    21061 tgaataatga aaaactaaaa gaacaatgta aaaattaaga aagagagggg aagaaaataa
    21121 gataatatag ctcaaggctc ctattcttga actttgaaat aaaattgtca ttcttttcag
    21181 ttagaaaaat acaatttaaa aatatcattt agagcaatat acaaacattt ttgttctctg
    21241 agatttcaat atttgtactt ttccttatgt ggaataagaa ataatggaat ttcagcacat
    21301 aaaaaagcct gaatgaacta catcactcag gcaattaaaa aatttcaaaa aatatttttt
    21361 gcttttagct ttctgaagtc ggaggaacaa ttccggccaa ttcgggatcg accagaatgc
    21421 ggccacaata ttcacacact ataatttttt tgcgcgagcg aatatccaac tgtcgttgtg
    21481 caggaatttt attgaagcat cctccacaag catcgcgctg aacgggaaca accgaaagac
    21541 catttctcga atttttacga atgcgtttga atgccgtcaa aagtcgaggt tcaactcgtt
    21601 gttcaatttg ttttatttgt tctctcaatc tctcctcttc ctgtttggtt tcagaaataa
    21661 tttcattcaa ttctcctttt ttttgctcca aatccaagcg tcgttcggat aaggcttcca
    21721 ttgtgctttg ctgttctgcc tttttagctt cgagcgctgc agtgtattct ttgatacgtt
    21781 tttcgcacaa ctctatttcg agcgtctgat attcaatttc tttggaaaga ttgtcatatt
    21841 cacgattatt tctgacattt tcctgttgct ccttgtactt ttctatttta gtacgagctt
    21901 gctctatttc caattttttg gctttgatgg cagcttccag ttcggcaatt tcatttttat
    21961 aattttccag tcgggtattc aaaccaatga tttcatcttc aaggtcttgc acttccaacg
    22021 gaagttcatc tctcaaattt ctgatttcat caatttttga atacactctc tgtagttcat
    22081 ataatgccct gagcttttct tcaacgctaa tttctttttc tgtttttggt tctgtagcca
    22141 tattgattac aaataattaa tgggatttgt ataggtttct gagattttga caacaaagtt
    22201 aggaattttt ttcgagataa tttcataaaa aatatctttc gtaaattgtt cagattcata
    22261 atgtccaaca tctgcaatca aaatttttcc ttcagcatta aaaaattcat gatatttaaa
    22321 gtccgcagaa atataaatat cggctccctg ttgaacggca tctttcaaaa aagcgcttcc
    22381 tgctcctcca caaacggcta ccttaacaat atttttcccc aacaatggtg tatgacgaat
    22441 gcaaggaacc gaaaaaacgg ttttcaaacg atgtagaaaa gcaagttcat ctgttggtat
    22501 ttcaaggtta cccaatagcc ccgctcctgc cctgttccat tcattttcca gaggaatcaa
    22561 atcataagca ggctcctcgt aaggatgagc ttgtaacaac gcattaacaa ccgaattttt
    22621 caaataactc ggtaaaatta cttctattct ggtttcgggt tctgcatgaa attcattgtg
    22681 ctcgccaaca aacggatggg catttcttgg gctttaaaag ttcctgttcc ttctacatta
    22741 tagctgcacg aatcgtaatt gccgatgctg ccggcacctg cttcaaacat tgctttccta
    22801 accttgtcgg catgagcatg aggaacaaaa gtaaccagct tgagcaattg atgagacaaa
    22861 ggttttaaaa cttttgtatg aaccaattgc aatttttcgg caattttttg gttgactcca
    22921 ttccaaagat tgtccacatt ggtatggcca gcgtaaattg ccaaatcgtg tttcaacgca
    22981 aaaataacgc aacgttctgt ttcattctga ccaacaagac gttttaacga attgaaaatc
    23041 aaaggatgat gagccaaaat aaaattgcat ttttttcgct tggcttcttc caatacttcc
    23101 atcgtaacgt cgagactaat caaaatacca ttaacttcca tgaaaggatc gcccactatc
    23161 agccctgcgt tatcataagc ttcctgtagc gccaatggtg caaattcttc cagaatattg
    23221 caaactgttt gaactttcat ggctgaaaac gaatttattt ttctgaagaa atttcttctg
    23281 ttttatctga tacggtttca accttgtcgc cggtttcagt ttcagatgct gcaaaatcgg
    23341 tcaatttggc atctaccaaa atgttgtacc aattgattaa tttccggatg tcgcttggat
    23401 aaaccctgtt tttatcataa tcaggaagaa tttcttgaag gtattgaacc agcttttcat
    23461 ttcccgattt agcatcgaca gcacattttg ctccattttc tttcaccttg attttctcaa
    23521 aaacttcaga caatggaatt tcgccatttt cagtaaaaat annnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23641 nnnnnnnnnn nnnnnnnnnn ncttgatttt ctcaaaaact tcagacaatg gaatttcgcc
    23701 attttcagta aaaatagcta tatctttcaa atatactaat ttgtcgcttg aaaaaaccgg
    23761 aatacgtttt ccatcgctca gggattcaat aaccatgaga ttttttccct gtgataccat
    23821 tttgtaaaga cccggtttgc cggaaacata taaaatttct ttcaacataa cttattaatt
    23881 acaaataatt tgactatttt aatgtttaaa atttaaaata aaaatcgact taactcttgt
    23941 aatgaaatat taccctcgta aattgctttc ccgactatga ctgctggaat acctgcttca
    24001 tttaacattt ctatatcgtg aagtgaaccc actccaccgc ttgctattaa atgaacctga
    24061 ggttcggcag aaattatttt tttgtacagt tcaattgccg gtccttgcaa cattccatca
    24121 cgactaatgt cagtacaaat aacgtattta actccttttg aaaaataatt atgaacaaaa
    24181 ggaatcaatt ccaattcagt actttcagtc cagcccgata cggcaatttt ttcgttttta
    24241 acatcagctc ccaaaataat tttttctccg ccgtattttt cgagccagcc aacgaagatt
    24301 tcaggatttt ttacggcaat gcttcctccc gttaccattg aagctccaca ttcaaaagca
    24361 atacgcaaat catcgtccga cttcaatcct ccgccgaaat caactacgag gtcggtagta
    24421 gaagtaattt tctccaatac cttgtaattg acaatgtgtt gagcctttgc tccatccaaa
    24481 tctaccaaat gcaatcgtct gaaaccttca tcagcaaaca ttttagcaat ttcatcggga
    24541 tagcggctgt agagcgttac tttatcgtag ttgccttgag acaagcgaac acatttaccg
    24601 tcaataatgt caatggccgg aataatttcc atttatgatg ttttttgtat ctattttctt
    24661 caaacaagtt ggcaaaagta taaaaaaaga aaatgtgaaa caagtcaaca actattttga
    24721 ttatagctgt gaataatcga tatttctaca aaataaacag ctaaaaaatt catcactcaa
    24781 aaaattacgg aaacaaatat tttctgatat ttaatccgtt atttttcaga aactcaaaag
    24841 gaacacttat tccaatggta caccaacggt tcccatcaga tgtttgatta tttgctttga
    24901 tcagatgaac aatggtttgc tgatcgaaaa aattcatgtt tttggaaata atcgaagtag
    24961 cggaacaata aatttccttg taccattcag caggaatcat tggaaacagc atatccacac
    25021 aaaattttct gagattttta gcattgcacc aatagacgac ttcgggaaca aaatacacca
    25081 acatatcggc caaaccggtt tcgagccaac cacgcgtatg aggatatttg ctaaaaactt
    25141 caatatacaa atcgacgaaa tccttctctc tgaatttttc ggaaacatgg cagactcgat
    25201 tggtttttat cagttgcacg tcgatatcct gaagctgcat tttcatatct tctttcgagt
    25261 ctgttgcaaa tcgtatgatt tcatctacga gtaaaacttc acgataaaac gcatcggcgc
    25321 gccgaaattg acttttttcg taatgaagac tttttccaaa ctcatgcacc atgaaaatca
    25381 attttaattt tcagtctttt ttctcgaacg caattttttc aattcgtttt ccaaacgttc
    25441 aacttctttt tcgagttcgt aaatggtatt gagcaaagca ttaagttctt cgttatcaat
    25501 agtagtggga tattgttcat caactctcag cagaaaatca gccaaaatat tcatataatt
    25561 cataaacgca tcatagggcg attcgtaata agttccaaaa gcgtccttat gactcatgta
    25621 tcgaaaatga tcggatgact gcaacataag ccaatcgtgt cgaagcggtc tgtcggtaca
    25681 aaggcgaacc ctctcgccta ccgcataaag tttttcgagt gcttcctgct gaaggtcgtt
    25741 tccattccac aaacttatgt ctttttctat tccggaccaa ctaatgggtt cgggagaaga
    25801 aagaatatca actgcttcaa tcttttttgc cacttccgaa ggcattaaaa aaccgatatt
    25861 gttttgcaag gcaaaaaaag gaatagcttt gagaaattca aaaattccac tttcaggccg
    25921 ctgaaacaaa cccaatgctt cgtagccaag ccagatattt acaacaggct gatcatcggg
    25981 catatttttt atccatgaaa taaatttgtc ggctgtaaga gggtaatcgc tccacgagcg
    26041 atcagaaaaa tgaaaagcaa tatcgtcgct caacttaaaa ttacgaacca gcaattttaa
    26101 ttttggtaac gtcgggtgcc gataaacgaa atttggactt ttccaaccca aaacatggct
    26161 gacttcatca atcaatatgg ttctatatcc catattgtag attttttctc caatttcgtc
    26221 cgagtaaatc aactccgaat ttcggaatac agtcggtttt tttccaaaaa gtgactctat
    26281 tttttcggtt tgcagggcta cttgctgttc aaattcatgt atatcgtaaa cagatgaaag
    26341 agaatgagca taagtttcgg ccataaactc cacacagccg gttttggaaa gttcttcgaa
    26401 actgtcgatt acttcgggag catactgttc cagctgttcc aaaacaattc ccgaaaacga
    26461 aaaagcgcaa cgaaattttc cattggaatt ttggataatt tccaaaagcg tgcgattggc
    26521 cggcaaaaac gactgttcag ccaacatacg aattcgttct tcattctgaa aatcatcata
    26581 ataataatga tcctgttcaa tgtcgaagaa acgatatctt tttaatcgca ttggctgatg
    26641 aatttcaaaa taaaaacaca gatttttcat acatttatcc ttttttcttt aggaatgaaa
    26701 ttggatttac ttttttcaga tatttcagct ccaaaaattg tgtaaatcta ttcaaacgat
    26761 aaagattggt ttaatttata aatttgatta tcaataagac aattttctta ttgaggttga
    26821 tttctaaaaa agaaaatttc tttaaaggaa tttatttccc caacagacta ttgtatattt
    26881 taattacttt tttacctgca tcttcccaac gaatgttgtt tacttcttct ctccccaatt
    26941 cgtgaaggct tttatacatg gccggattcc tgataattgc atttactgca tcggccatac
    27001 catcgatatc ccaatagtca attttaattg aatgtgttaa aatttcagaa catcccgact
    27061 gttttgaaat aatggtcggt gtacccactt gcatggcttc taaaggagaa atgccaaaag
    27121 gttcagaaac ggaaggcata atatatacgt cactatcggc caacatttga tacacttgtc
    27181 ttcccttaag gaaaccggta aaatgaaaac ggtcggtaat acctcgcttt gcagccaaat
    27241 taatcatatc ctgcatcata tctccattac cggccatcac aaaacggact tttttatttt
    27301 ttttcagcac acgattggct gcttctacaa aatactcagg ccctttctgc atggttatcc
    27361 ttcccaaaaa agtaacaatt ttatcacttc tcggtgtttt aacataagaa tctacgtcag
    27421 gaagaggctc tacggcatta tgaacggttg taaccttttt cggatcctgg aaatactttt
    27481 caatcacaat atttcgggtc agattgctta cggtgataat gtgatttgcc gcatccattc
    27541 ctcttttttc aatatcgtaa actatgggat taacatttcc cctactgcga tcaaattcgg
    27601 tggcatgaac atgcaccacc aaaggttttc cgctgatttg tttggcaaaa atacctgcag
    27661 gataagtcaa ccagtcgtgt gaatgaatca catcaaaact caattgatgt gccaacacac
    27721 ttgccacagc ctcgtagttt gaaatttctt ccagcaaatt gtcgggatac cgaccggaaa
    27781 attctataca tcccaaatca ttggtataaa ttctgcgaaa atcgtacttg ataccatttc
    27841 tcagccagaa atactcgtca ggattcatga tccagccaat acgctcgttg acataattcc
    27901 aatcgttttc tttccaaacc acagggacat aacaagcccc aataattttt aaaaaacttt
    27961 ggtcttcatc tccgtagagc ttgggaataa caaaagtgat ctccatatcg ggctggtgtg
    28021 ccatagcttt caccaatcca tacgaagccg tacccaaacc gcccaaaata tgaggaggaa
    28081 attcccagcc aaacattagt actttcatac attcttcagt ttttcatcgn nnnnnnnnnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aggaaattcc cagccaaaca ttagtacttt
    28261 catacattct tcagtttttc atcgtatata ctcacctcta aactttccgt ttacaccaaa
    28321 ttcagtggta ttagtcttct ttatattctt tcaaaatttc cattacacgc aatacttctc
    28381 ccacattcat ggcaaaagac attccgccgt gtcccttaaa aggtggattc ccgtcatata
    28441 attcggaaag tgtaccgaca cacaattctt tcatttctgc ctcgaagcca atcatcattc
    28501 tttcgataaa agattttcca ctcattttgt aaatttttaa ataagcttca acaaatgcac
    28561 caaaagtaaa aggccaaaca ggcccgttgt ggtaatttcg gtctctttcc aactgtccac
    28621 ctatgtacac cggacgatac ataccacttt tagggctaat ggttcttaat cctttcggtg
    28681 tcaacaattc tttggttaca atatcgagaa tcaacttctg ctgtgatttt tcaagaggag
    28741 agaatggtaa gcccactgca taaatcatgt ttggtctaac ttccttgtca ttgtaattgc
    28801 cgttaacaaa gtcgtaaaga tagtttccat tccaaaaagt ttttatgaac gattctctta
    28861 atatgcctga ctgatagtct attacattgg cttccatctc ctctcccatt tcaccaagaa
    28921 gttgagaaga aaatttcaaa gcattatacc acagtgcatt aatttcaact acgtaacctg
    28981 ttcgaggagt aatgggtctg ccattttcta ccgcattcat ccatgtagca ggtttttcca
    29041 acccattgac ataaagcaag ccgttgttgt ggagaaaaag attgggatgc cgttgtttgc
    29101 gaacaaaaga aataattgaa gcaaccaatt ttccgaaacg ctgcgctgtt tctgaaagtg
    29161 aagtgtgacg agcatattgc tgaatggtcc atacaaacca cagcaaaacg tcgggtgcat
    29221 cgatttcttt cagtttggag ctgactttat tttcgttaat aaaactttca atttcaggaa
    29281 tggctgtttc aattatggca tcgaaataat ccattctgcc aatcgacaaa gtacaaccgg
    29341 gtaaagcaac aaattcatct cttgcccttg ccgtaaacca gggataacct gccaataaat
    29401 aagttttgtc gccttcgcgt ttataaaatt gttgggcaga atttttcagt gcactgaaca
    29461 tatcacagcg tggatttctt ttttccaatt ccttatccca aagctttttc agtgatgaag
    29521 ttgagacttc cgatattcct gctgaaaaaa taacggattc tccttttttt atgggaattt
    29581 caaaagaacc cggcaccaac aagtcttctc gatattcaaa acctctttcc ctttctttgt
    29641 aatattctat atttttatac cagtcgggga aatgctgata aacattcttt ttggaaagtt
    29701 gcatgaaaag cgtaggataa ttttcataaa gcttcatggc tataccgttg gaaacaggtt
    29761 gaaaatcaca gttggcttct ggattctgat gggtaagttc atgaacattt cgaaaagcca
    29821 gaaaaggctt aaagcgtaac aaagttggcg aatgagcttc caacaaagta tatttaaaaa
    29881 ggacacgcgg ttcaaatgaa accagcatgc gttcttttga aagaattact ccccccacac
    29941 gataaattgt tctcggaata atttcgcaat cgaattgacg aatgtatttg tggccgttgg
    30001 gataaaaatt gttgccttcg tactgatgta cccccaaatt aaactcaatt ccatgttgaa
    30061 caatggtttc gtccaacgaa gagagcaaca catgattggc ttcgcccaac tcgggtatgg
    30121 gcactaccaa ttgcccatga tattttctgg tgttgcaatc gatcaaggtt gaactgttgt
    30181 aagtgcctgc acgattggta cggagaattt ccttcgaaag agaccgttca aggttaatta
    30241 acaaggattt gtcgaaactt agataactca tctgattgtt attcttagac tatctgatta
    30301 tcaattaaat acaattttct tttaaaaagg aatgtaaatt agaaacatta caaaaatcaa
    30361 tccttagata gattttaata ttgcttgcaa tttatcgaaa atttccaatt aaaaaaaagg
    30421 aagttgataa ttttttcaag gtgacgaaaa aaatttcttg gttcccaaca aatccaaatt
    30481 tcattgatac atgcaatttg ttgtttttca aatttttata aaattcatca aaaaaaatga
    30541 tggctgaatc gtagagaaag ttcatttgac agttaaaaat tatcttctca aacaaaaaaa
    30601 tttttacctt tgatgtaatt tttagtgttt ggaagacgtt atttaaaaca gagttgcgaa
    30661 atgacgagca acatcgagag tgaaagagtt ctggagaagc aacggtggaa attcgccatt
    30721 attttgcctt caatcgtatg tattttgtgg atattatgtt ttgtattgga gcgcggcatg
    30781 ggttgggatt ttcataaagc cggcatttat ccgcgaaaac tcgaaaacat ttggagtata
    30841 tttacttaca gtttcattca tgccgattgg aagcatttaa tgaataacgt tctttctttt
    30901 tttattctgg gaatatgcct gtattatttt tacagtgagg ttgccaatcg agtgctaatc
    30961 atatcggccg tgctcagtgg attgattttg tggtgtatcg gacgcgaaaa ttttcacatt
    31021 ggcgccagtg gcattattta ctcgctggct ttttttctgt ttttcagcgg aataatcaga
    31081 aaacatatcc ctttgatagc catttcgttg attgtcgtgt ttatttatgg tagcatggtt
    31141 tggcatcttt tcccctggtt gccggatgat ccggtatcat gggaaggaca tttatcggga
    31201 gccgtaaccg gtatcgtgct ggcatttgtg tacaggcaca gagggccaca accacccaaa
    31261 cccgaatggg aagaagatga ggaacttgat gaagaatacg aatgggaaga aaattatgat
    31321 caagagcagt ggaaagaaga atgaaacaat caattttgac aaacaataat tttaatcaat
    31381 atggacgaga aaaatacaaa agaaattaat ttgctggatt tgataaaaat gttcaatgct
    31441 tgggttgtaa aagttggtaa atcttttatc aacttcattt tgtatctgct gaaggcaggt
    31501 tatcgaaaaa aatatttgac cctgactctc attgtattgt gttttgccat aggcgaatac
    31561 atggctcgac caagtaaccg acagtatgcc gccgacgcta tggcttcgct gtatggcgta
    31621 gatgctttta ccatgaagga aattgcaaaa caattggaac aatcgagagc caataaccaa
    31681 tacacttcac ttggaaccaa attgttgttg ccggacagta ttgcaaaaaa cattgtttct
    31741 ttcaattcct attttgtaat cgattatctg aataattcta ctcccgatat ggttgatttt
    31801 gccaataatc attcactgga agatacttta aacgttcgga tgaaagataa aatatatttc
    31861 cggctggtta cccgcaatat ttcacaagta ccacaggttg aaaaggctat attaaatttc
    31921 ttcaattcac aaccaactgt caggcttctt tatactgcaa gacaaaatga actgaaggga
    31981 aaaattgatt tatcttcggc tgaaatgcaa cgtttggaca gccttgcaaa aatttcttat
    32041 tttaaggaca acgatcaaca gctgaaatgg tcgaaagacc gtttgattgt gggcgaacag
    32101 caaaaacaac ttttttacgg ggatatgttg ttgcttcaaa aggtgaaagc ggaagcagaa
    32161 tcggaattac tgacttataa acagcctgtt gaattgcctt cgggatttgt agtcaatcct
    32221 tttcctatga atggaagact caaatacgga gtttatggta ttttgatagg tttgggcttg
    32281 ggtttggcgc ttgccgtctg cctcgaacaa ttgcaaaagg tggttcaatt ttttagaaaa
    32341 taaaaatacc tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcctcg
    32461 aacaattgca aaaggtggtt caatttttta gaaaataaaa ataccttttc tgtacattaa
    32521 aaaaacaata tacctaattt tgaagtagag cacaataaat ttatttatac aattatattt
    32581 tacttgtttt caaaaatata gtttatacaa gctttcatac cacttcgtag caggaacaaa
    32641 aacttctttt gattttaaca aaagaattcc ccgcgaaaac tcttttatag agcattttag
    32701 tacttaaagg gacttttgtg caataatttt ttcaaaacgt taacggctac aaaataagcc
    32761 tcgttacgaa tgtaaaaaat ctcatcaccg gtaattttta tattcaagaa cggtaacgag
    32821 taacacaaac ttaccggtat tgttataaat atacttgcct gcagaaaaaa atatttggac
    32881 gcagggtgaa gtctaaggct ctggttaagg gcaaaattct ttcctttaaa acctcaaatc
    32941 cgattaaaaa ctaaatggat tattcatctc caaatgctca ctgctaattt accaagaaaa
    33001 attttcactg caatctaaat gagttttgat atgaatttta cagatgatac gccgatgtta
    33061 tcttcgcaaa gttgctgttt gctgttataa attacaatag gtcattatca agataatcat
    33121 ttttttttta attttgttga actttaccaa tcaaaaagac gaataaagaa acatcatgaa
    33181 acaaaaattt ctgagcattt tatttttatt tgtaaactat tcattcctca taagtgcaca
    33241 aaacaactta tcgacaaacg atactggtga agttcaaaca atcaaagtaa ttgatttgga
    33301 cagtttgcta aagaatacaa aatggataca aaacaacgaa acaaattcgg aaatcataga
    33361 attgtcagaa gattcagata cattgaaaac aaaacattta gaaaaaataa aagttctgaa
    33421 agattctttc caactgtttg tttctccaac tgctttagct acgattaaat cggattcttt
    33481 gttatattta tccaatccct tttttattga gctggtctat atgggtcttc cttttgattt
    33541 tgatataaaa cctaaagaag atttactcac attatttcac gagcgaaaag cttccacact
    33601 tgaagaaaat gcttatcgac ccataaaagt acctacttca caagaatcgt taattaaatt
    33661 gcgtcaggat gcaagaaatg aaattaccag aacagccgtc ggattatata aaaggatgtt
    33721 ctacgagctc tcaaatcctt atgtaaaagg taatcggcct attcaagtaa aacctataga
    33781 aaaagttgag tttgttgaca aaaataaata cgtgaatgga aaagaaagaa aaatgcatgt
    33841 tcctgaattt caagttgggc cttggcgaca taaaggttca tttctgctac aattttccga
    33901 aaattatatt tcaaaaaact ggtatcaggg aggaaacagc aatctttcgg tattgggcat
    33961 tttaagcggg caaattaatt atgacgacaa aaaacgaacg caatgggaaa acagtctgga
    34021 gtggagaagc gggttttatt cagtttttgg tgatactaca gcactgcgaa ccttaaacac
    34081 caatgaagat gttttgaaaa taaacagcaa atttggctat aaggcaagtg gcaattggtt
    34141 ttatagtgga gcgatagatt tctctactca atttttaaaa aatttcaagt cagtcaattc
    34201 taacgaatta aaagcttcac tgtttacgcc aatccgttta aatgtcaatg ttggtatgga
    34261 ttacaagtat aaaaagattt tttcgctcat ggtgtctccc ttgtccttaa aatatatata
    34321 tctaagcgac actgtcaagg ttaaccccaa tttgttcggg atacaaaaag gaaaaaatca
    34381 tttagagcaa attggtagtt cgtttaaagc ggttgtttct tatgcaccat caagagaaat
    34441 acagctcgat tccaagtttg ctttttatac aaactatgag aaaattgaag tcgattggga
    34501 aatagtttgc aatatgatag taaatcgttt tgtctctact cgtatttcga tcaatccgcg
    34561 ttacgacaac actatgataa tggcagaagg cgaaagagcg aaggtacaat tcaaacaact
    34621 actcagcgta ggattttccc ataaattcta acaaaccgga ttaaccaaaa agttgatgcc
    34681 aagaatcaaa tttctcgact ttttccttcc cataaaagct taaattttgc ctaatctctt
    34741 ctaccgaaag ctggagattc aattttgatt tggaataaaa tttgtcggca tagcatatta
    34801 actgttcttc gagtgaaagg ggaagcatat cgcgatcagg gagaggaagt ttcttgagtc
    34861 tgatttcttc tttagtaaaa cccgttccgg tgtgtctttc ggcaacaaga gcatgttgtg
    34921 gaagcccttc ttttttcaat aaatctgaac ccaaataacc gtgttcgata tattcatgtt
    34981 ctccaaaaca ataaattcgt ggtgcatgac agagaaaaat tccgatgtcg tgcagcagac
    35041 aagcttcccc cacaaattca gtgtcgatgt taagttccgg atgttgctta gctacttcta
    35101 atgccttgtc tctgacttgt tggctatgaa taacaaggac acgataaaga ggagaatctt
    35161 taacgtaata tttttcgata attgaaaaag gatgaataac cataataaat tgttaaaaaa
    35221 gttttttcaa tcaattcata aaaaataaca agagaatata cagtttattt caaaaaaact
    35281 tatcaaataa aaagcccact gttaaaaaca caatgagctt atttgtaaaa aaatcaaaat
    35341 caaacattta agaaagtata cccagcaaaa tacctgctgc cacggccgaa ccaataacac
    35401 cagcaacatt gggtgccatt gcatgcatta ataagtggtt tgatttatcg taagacaaac
    35461 cgacatcctg tgaaaccctc gcagaatcag gaacagcaga aacaccggca gaaccgataa
    35521 gaggattgat tttgttccct tctttaagga aaagattcat aatcttggca aaaagaacac
    35581 ctcctgcagt agcgatcata aatgagaaag ctcccaagcc aaaaatcata agcgaatcaa
    35641 cagtgagaaa acgatcggct tgagtagaag caccaacagt tacacctaac aatatggtaa
    35701 tagaattgag taaagcattg cgagccgtat cagccaaacg ttcagtaaca ccggattctt
    35761 tcaaaagatt gcccaaaaac aacattccca acaaaggcat agcgctgggc gaaataaagg
    35821 ttgtcaacaa caagccgata atggggaaaa taattttttc caatttggta acttgtcggg
    35881 gaggtttcat tctgattttt cgctccttgt ttgttgttaa aagtttcatt ataggtggct
    35941 gaataacagg aaccagggcc atataagaat aagcagccac cgcaatagct cccataagtt
    36001 cgggcgctaa acgagaagaa ataaaaatag ctgtcgggcc atcagcacct ccgataattc
    36061 caattgcccc agattgggca ggtgaaaatc ctactgcata agcaataatg aaagaaacaa
    36121 aaattcctaa ttgcgcagca gcaccaagca tcattaactt aggattggaa atcagagaag
    36181 aaaaatcggt catagcacca atacccaaaa agatcaaggg tggataccat ccttgtacaa
    36241 ctccgctgta caaaatattc attaccgaac cttgttcata aattccgatg cggaaaccgt
    36301 cagtaaaagg aacgtttcca atcagaatgc caaaacctat tggtacgaga agcaaaggtt
    36361 cccatttgtg tgcaataccc aaataaatga aaaccaaacc taccacaatc atagccaaat
    36421 acttaaattc aaaattggct aatccagtat tgttccaaaa tttctgcaaa ctctctatca
    36481 ccagattttc actgcctgac gattgtgcaa aaatactgat ggggaacaaa cttatcaaca
    36541 atagggcaag aaaaccaatt ttatatctaa aatttttcat tccttcaata ttattcgatt
    36601 tcaaataaaa cttcatcttg taaaacggct tttcctaccg aaacgcaaac ttttacaatt
    36661 ttaccattgc gctcggcaag aatattattt tccattttca ttgattccat caccagcaaa
    36721 acatcccctt ctttgaatgt atccccttct tttacgttta ttcttaaaat atttcccgga
    36781 agtggagatt ttattaccgt agagctgggt ttttggttag aaggaacggg attaaactta
    36841 tcagcaacat tcatattact gggagttgtg ggcacctctt ttcttacaag tataggagtt
    36901 ttgctcactt tcacctcttg attcaatgtt acggtgtaag gtgtaccatt tacttcaatt
    36961 tcagcaatat ttccatctat agattttata taaacatgaa aatcgttacc attaatatta
    37021 aaatcaaatt ttttcattat cggtgtaaat tatttagtcc ataaattttt gaattccatg
    37081 gcgaataacg cctttctatt cgtttaatgg taagcacatt gctttcttca tcatgcatac
    37141 tgtctaaata taaatataac gccatagaaa tggctgcaat ttcattggcg ggaatatcct
    37201 ttattttatc gacatctata cctgatttca ctactttctt ttttgccact tttaaaacta
    37261 ttacctgaaa aagttcaaaa agcagtacca aaacgaataa gacggaaaaa acaaccatta
    37321 tacctattgc agctgctgta aggagttctt cccatgagtt gatcatcaat aatttcatta
    37381 tcatttttta taaaggaata ttacaatgtt tttttaatgg attcacgacc ttttttgttt
    37441 gcaaagtttc aaaagcacga atgattctga aacgtgtatt gcgaggttca atcacatcgt
    37501 caatatagcc ataagaagca gcctgataag gatttgaaaa tttttcgttg tattcagcaa
    37561 ctttttcagc aatatatttg gctttttctt cgggatctgc aatattagcg atattttttc
    37621 cttcgagaat ttcaatggcc cctgaagcac ccataactgc aatttcggca gtaggccatg
    37681 cataattaaa atcaccacgc agctgcttgc aactcattac atcatgtgct ccaccatagg
    37741 attttcggat ggttactgta actttgggca cagtagcttc accgtaagca aacattaatt
    37801 tggcaccatg aatgataatt ccggaatatt cttgaccgga acctggtaaa aaacccggca
    37861 catctaccaa tgtaagaata ggaatattaa aagcatcaca aaaacgtaca aagcgtgcgg
    37921 ctttacgcga agcatcacaa tcaagtacac cggcaagaaa attgggctga tttgcaacaa
    37981 tacctattga agctccattt aagcgtgcaa atcctactat gatatttctt gcataaaatt
    38041 gatgaacttc caaaaattct ccgttatcta ccaatgaatg aataatttct ttcatgtcgt
    38101 aaggcaaatt cggattgtca ggtacgatgg tattcagttt gtcttcatga cgatcgattg
    38161 gatcatagtt ttgaatcaat ggaggttctt caagattatt ttgaggtaaa tacgataata
    38221 atttacgtat catcagcaac ccttcctctt cgctttcaac cttaaaatga gacactcccg
    38281 actttgaaga atgaacattg gcgcctccaa gatcctcagt tgtaatatct tcacccgtaa
    38341 cagatttaac aactttggga ccggtaacaa acatgtaact gttttcctct gtcatcaaaa
    38401 taaaatcagt aagtgccggt gaataaacgg ctcctccggc gcaagggcca aaaatagctg
    38461 aaatttgtgg tacaagacct gaagcaagaa tgtttcgttc aaaaatttcg gcatatccgg
    38521 ccagtgaagt taccccttcc tgaattcgtg cgcctcccga atcattgatt ccgataattg
    38581 gagcacccat cttcatagcc atgtccatca ctttgcaaat tttttgtgcc atcatttccg
    38641 aaagtgaacc accaaaaacc gtaaaatctt gggcataaac atacactgtt cgtccatcaa
    38701 ttgtaccgtg tccggttact acaccatctc ccaagtattt ttcgttttcc atcccaaaat
    38761 tggtacaacg atgtgtaaca aacatatcaa gctcttcaaa acttccatca tcaagcaaca
    38821 tttcgattct ttctcgcgct gtatatttgc cttttttgtg ctgtgattca attttctttt
    38881 ctcctccacc cagcttggct tttgcacgta aactgattaa ttgttctaat ttttccagat
    38941 tattcatttt ttctttttat cttaaagatt ttttaaaaat aaatcaacaa tttatattgg
    39001 gattttcaca caactcagtc aaaattccaa aagtagattt tggatgtata aaagcaatat
    39061 ttaaaccttc ggcacctttg cgtggttgtt tgtctatcaa cataactcct ttatcggatg
    39121 cttcatttaa ggcttgctga acattttcta ctgctaaagc aatatgatga actccttctc
    39181 cgcgtttttc aataaattta ccaattggtc cttcaggatc agttgactcc aacagctcaa
    39241 ttttagtgtc tccaacctta aaaaaagctg ttttaacttt ttggtctttc acttcctcta
    39301 tagcataaca tgtaagtccc aatacgcttt caaaaaaagg aatggcagtt tccaaacttt
    39361 ttacggcaat gccaatgtgt tcaatttttt ttggtttcat aaaatgaaaa atatagttgt
    39421 gtgttaaatt attttattga taatcaataa aataaaatta aaaattgtaa ttacagaaag
    39481 gtcaattagc gaaatatttc cttatttaat attttctgca aagttaacaa tataattgtt
    39541 tttcaaagaa taatttcaat ggaaaaaaca tagatattta aaagtttttg acaataaaat
    39601 tttttttctt ttaatttact ctttttacca gtttcatccg aattaaatat taaccatttt
    39661 ttcaaagcaa atagaaatca aacttcgaag agccctccat agtttggaaa acaacgcgta
    39721 ccttttttaa aaacaatttt tttccttttg gaaattttag attattgcaa tttcagagcc
    39781 ttttgcacta aacaactgat ttgtcttttt tgatagccaa ccctatgcct ttttatttga
    39841 atctttgttg tatttttacc caaattttgt aactttgttc tacggttaaa tgtttctttg
    39901 tttgtaattt tttagtgaaa agaaaccata gaaaaccata atctaagtgg gcaaaccgga
    39961 ataaatcttc ttctttttta tcttgaaatg attttccttg attttttatt cccctaaaaa
    40021 attgcattta ctgattgaat ttattgatca aaaaaaatga atgttatgaa aaaacttttt
    40081 ttactgtcca tcatgatggt ttttacattc ttttcgttcc aatcggggca aaacaaaaaa
    40141 ggcgtggaaa aatctcaaaa cactctgcct atggacttaa aaacatttga cactattcaa
    40201 ttgcttgcac ctgatacaat gggtggaaaa tctttgatgc aaacttttca acatcgcaag
    40261 tcagatcggg aatttaagca aacgccattg tcattgaagc acttgtccga aatactttgg
    40321 gcagcttatg gcatcaatcg tcctgatgga aaaagaacgg ttccctcggc aagagctctt
    40381 tatccactgc aagtttatgc agtacttccc aatggaattt atcgatacga accacaaaca
    40441 catcgacttg tgcctgttgt aaaaggagat tacagaaaat tggcaggctt gcaggatttt
    40501 gtttacacgg ctccgatgaa tttgatcttt attgccgact tcaacaaata caatttgacg
    40561 cagaaagaac ctgacgcgaa tagcatacga atggtttcgc tcgatgccgg acattgtacc
    40621 caaaacgttt atttatattg tgcttctgaa ggattgaggt gtgtggttcg tgccggagca
    40681 aaggcagatg aactgctttc tctgctaagt ttggacagtc attatcgctt tattgttgct
    40741 caaacggtgg gctattgacc ataaaaatca aggtagtctt tctgtgttca gagcgttggg
    40801 atgttcattc ccgtaatttt tctgtaaaga tccagtgcat aaacatcggt cattccggaa
    40861 atataatcca gaatggccat tacttttccg taagttgtcg gctgatcaat ctcatactgt
    40921 ttgggaatgc gcatgagcaa ttgctgagaa tatgcttttt cgggactcag caccgctttt
    40981 accaaatttt cgagtaaagt aaaaatgatt ttataacctg caagttcaac atcaagcact
    41041 tccgaagaac agtaaatttt ctcaaaagcc gtttttgaac agttttggta agctttcagc
    41101 gacacgggag gcagcagttt tattaatgtg gaactgaaac tcccttccag aatttgaggt
    41161 tcattttcca gaaaaacaga agaacagttg tcaatgagct gtccaataac cgaagaacgc
    41221 aaataggcaa tttgttcgtt gacatcatca accatagcaa tagtttgcag ttttctttcg
    41281 agtttcttcc cttcaaaata ggaaaggaaa agagcttttg tttcgtcggt ggaaagaatt
    41341 ttcaatttgt gcgcatcttc aatatccatt atttggtagc aaatgtcgtc ggcagcttct
    41401 accagataaa ccaacggata acgaacatat ttgtccggtt gtgaaaacga atgaggaata
    41461 ccgagttcgt gagcaatttt ttcataatcg gatttttcag aatgaaaaaa accaaatttt
    41521 tgtcgttgcg gatccgaaaa aactgaagca tagggatatt ttactatgga agccagtgtg
    41581 ctgtatgtga gaacgaaacc acccggacgt ctgcctctga attgatgggt aagcacgcgg
    41641 aaagtgttgg cattgccttc aaaacttatt aagtccgacc attcttcggg caacattttc
    41701 tcctgcaaaa tttgtccttc tccttccgag aaataggttg ctatggcttt ttcgccggaa
    41761 tgtccgaaag gcggatttcc catgtcgtga gccagacaag ctgccgaaac aatgctcccg
    41821 acttcatcaa taaaagcatc gggtaaatta tttttctctt ttatttgacg tgccacattg
    41881 atccccagcg agcgccctac actggaaact tccaagctgt gcgtaagccg attatgaacg
    41941 aaaacgcttc cgggaagtgg aaaaacctgt gttttgttct gtaaacgacg aaatggagga
    42001 gaaaaaatca atcggtcgta atcgcgctga aattcgctgc ggttatcctg acgcttttca
    42061 tgataggctt ccatcccgag tcttttggtg gtaatcaatt gcttccagtt catattcacg
    42121 aatttattat tgataaaaca aatttcggtt gaaaacgtta gcgcctagtc ccaattgaag
    42181 ttgaaatttt tcaattcttc tttcaatctg tcgctttggt tgtcggtaac gcgattcaac
    42241 cagcgccaaa agtccgtact gtgattaagt tccctggtat gacaaagttc atgtaaaata
    42301 acatagtcga ttaagtgcgc cggtaaagcc atcagataaa gagacaaatt tatgttcccc
    42361 tttgcggagc aacttcccca gcgggtacga cttgattgaa tcttgacagt ggaaaacctg
    42421 aaaccaaatt tgtctgccaa cagtctggtg cgttggggta aaactctttt cgcttctaac
    42481 ctgagataac gagcaataat cctgtgtaaa taattttgca tttgttcttc cctgcaatca
    42541 acttccgaag gaatttcaat ggagagaacc tgatttttca gacaacaata gatatcttct
    42601 cgatgcgtgg gtttgatatc agtaacaaaa gtcagcgccc tgaaagggga ttgttcatca
    42661 ataatttgtt tgtttttgcg tatcttttcc tttacttgag ctcgtttttt taaaattttt
    42721 tcacgaaccg agtcgataaa tctcattgca tctttttcgc ttgcccgcat gggcagggta
    42781 actttcaatt tatcttctaa aatttgaatg tttatgcgcg tagcccgttg acttctgttg
    42841 aaataaatat cacccagttc ggggtcggaa atttttcttt gcgaagaaga agtattcatt
    42901 tgtgttgcga ttgaaagctt attttcgata tcgaaatcgg tattgagcag ccaaaacagg
    42961 ttctcttttc aattagaaaa aacaaagaca aatgtaatca atcacgccaa aattgaaaaa
    43021 atcatttttg gttgtcttgt aaatttgata agcaataact tatgaagaaa gtactatcaa
    43081 ttattttatt agcctttcca tcgcttcaat ggtgttttct ctgctgccaa atgccgagaa
    43141 acggatatat ccttccccac tgcgaccaaa acctgctccg ggagttgtaa cgatattttt
    43201 ttgtttcagc agaaagtcaa aatattccca acttttcata ttgtcgggtg cctttaccca
    43261 aacataggga gcattgacac cgccgaaaac ctttaaaccc aatttggcaa cgccttcacg
    43321 aatgatgcgg gcgtttccca gataatattc aataagttgt ttcacctgtt ttctgccttc
    43381 gggcgtaaaa gtagcttcag cgcccctttg tacaatgtag gaagtgccgt taaatttggt
    43441 agattggcgt cggagccaca atttgttcaa agcaaccgct tctccctgat gcgtataacc
    43501 aaccaactct ttgggaacaa ccgtgtaacc acaacgcgtt ccggtaaatc cggcagtttt
    43561 agaaaaactt cgaaattcga tggcaacttc tttggcccct tctatttcat aaatgctgtg
    43621 tggaacattt tcttcagtaa taaaagcttc ataagctgca tcaaacaata tcaccgaacg
    43681 attggctttt gcatactcca cccaaacctg aagttgctgt ttgctaagtg tggtgccggt
    43741 cggattattg gggtagcaga gataaatcat atctactttt ttatccggaa gctgaggaac
    43801 aaagttgtta gcttcgttgc agggaagata ggtcagtccg ttccattcgt taaattcgtt
    43861 tggttcgccg gctcttccgg ccatagcgtt ggtgtccaca tatacgggat aactcggatc
    43921 ggtaatggcc acacaattgt cgataccgaa aatatcgcca atattgcccg tatcgctttt
    43981 tgctccatcg ctgacaaaaa cctcatcaac atcgagtttt attcctcttg cccgaaaatc
    44041 attttccaca atggcctgtc ttaaaaattc ataaccctgt tccggcccat aacctctgaa
    44101 agtttccgca tgacgttgtt cttccgcagc tttgatcata gcatctaccg aagcgtcggg
    44161 caaaggaagt gtaacatctc caattcccat acggataatt ttagctgaag gattttctgc
    44221 cttgtgttgt tccacccttt tggcaatttc ggaaaataaa taggttggag gaatttttat
    44281 aaaattttcg ttgatataag ccattttaat gattcgatta atgatgataa aaaatacgtt
    44341 ttattttgaa aatcaatatg ttttgtagaa aaaaatgaaa acttaggaaa tttcgccttc
    44401 aaaaactgtt tcagccggcc cggtcaaata aacatgttca tcgttttcgt tccattctat
    44461 ttccaaattt ccacctaaaa gttccacagt agccttacgc gacacaagcc cgtttagcac
    44521 accggccaca accgatgcac aggcacctgt accacaagcc attgtttcac ccgatcctct
    44581 ttcccagaca cgcatttcta tgcggctttt gtcgatcacc tcgataaatt ccacattggt
    44641 acgacgagga aaaatgggag cattctcaat tttttttccc atcttttcga tatccatatt
    44701 ggcaatattc tccgtgaata ttacagtatg tggattgccc attgaaacac aagtaatagc
    44761 gtaattgatg ccgtcaaagg aaacagggaa attaacaatc tgttttcccg gaagcgtagt
    44821 aggaattttt tcagcttcca acaccggttt tcccatatcg actgtaacgg aaacaacatt
    44881 gtttctctcg tcaagaaaaa gttgcagggt tttgatacca gccaacgttt caagggtcaa
    44941 ttcggttttg tctgtcatac ctttttcata gacatattta cctatgcagc gcgcggcatt
    45001 tccacacatt tccgcttccg agccatcggc attgaacatt tgcattctaa aatcggcttt
    45061 tgaagaagga agaataatta ccaatccatc ggatccaact cctttgtgtc tgtcgctcaa
    45121 gcgtatggat aattcgccgg gattttcaag ctgttcttca aatccgttga tataaatgta
    45181 atcgttcccg attccgtgca tttttgtaaa tttcatgatg aaaataattt atgtactttt
    45241 ttcgatgaca aagataaaaa attaaatcag gacaggagaa aattataatt gcgattttca
    45301 aaacaagctg tgtttactta taaattatca gaaaacagct tcctttttga gagaattcaa
    45361 acaaaattca aatttaatgg ctgtgattga atattttgaa ctttcatcac ctatcggcca
    45421 ataacttttt caatgtttct gcaaattcat caatttcttc gggctttgta tcaaaagagc
    45481 acatccagcg caattcattg gtttgaaaat cccaagtgta aaatcgaaat ttttcccgaa
    45541 gaggctcaat cactttctcg ggaattatgg caaaaacggc attagcctgc accggtaaag
    45601 ttatttgaac ttcaggtata gcgcgcattt tttctgccag taaacttgcc attttattgg
    45661 aatgtgttgc cattttcagc cacaaatcgt tcttgagcaa agccataaac tgtgctgcta
    45721 taaaacgatt ttttgaatac aattgaagag attgtttctg ataaaaacgg gcattggcat
    45781 tcagttcatc gttaaaaatc aaaattgcct ctcccatcat cattccattt ttcgtgccgc
    45841 caaaactcat aatatccacc ccgcaatcaa cggtaacttc ctttaaactt tttcccagcg
    45901 ctgccactgc gttggaaatt cttgcgccat ctacatgaac aagcatgttg tttttatgtg
    45961 caaaatcaca taatgctttc agttcttcgg tgtcatatag cgttcccact tctgtggttt
    46021 ggctaatgga aatcactttt ggttgtgcgc tgtgcacatt gcctttgtgt accaaaaccg
    46081 gggaaatcag ttcaggagta agttttccgt ccggcgtatg caccggcata attgaacatc
    46141 cgatattttg aaaaactgca ccgcattcgt cagtcaaaat atgagccgtt tgggcacaca
    46201 caatcgattg aaagggcaat gtacagcact ttaaacaaac aatattggca cctgtaccat
    46261 tgaaaacaaa caaaacttcc acatttccca acttctgctg aaaaagtttt tttgcccgtt
    46321 gggtgtaagt gtcatcgcca taagaaattt catgatcggc attggcttct acaatggctt
    46381 taaggatatc gggatgaatg cccgaataat tgtcgcttgc aaaacatttc atataatttc
    46441 ttttttacaa aagtttaatg ataatccgca aagtatccta aattaaaagg ataaattatg
    46501 gtatattttt
//
LOCUS       DAOTAX010000009        44201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_707_length_44201_cov_30.456984, whole genome shotgun sequence.
ACCESSION   DAOTAX010000009 DAOTAX010000000
VERSION     DAOTAX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 44201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44201
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_44201_cov_30.456984"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..4868)
                     /locus_tag="PLM04_02040"
     CDS             complement(180..4868)
                     /locus_tag="PLM04_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HON01894.1"
                     /translation="MRRLSFYFQSMFIVLVTALQAQQIPPTNNTAFRYLKFQVDKAGT
                     STINYLEVGEVEWMVGTTAYPITKFTTNTTLVTSSATGEWGSSGINMYDGKINQAWVY
                     LRERPFPIWATLDNTTPIVPTSIKITIRPDCWFAGFSCYGSNDNINWYLLLDKQNYDK
                     ASTFGSGLTEKTVEFPIPAQLIDNQPPAVPTNIVASNVTSRSFDLTWSPSVDNEGGVG
                     FVTYEIYVDGKLRGTTQNTSYSVTGLSGNTFIVTIKAKDGAMPNNVSEASAPLIVQKY
                     IDPVLNNEPSMGQIMSKLYANRGQQIITLPQISDGEIDKKQTVTISVVSTTPELLTID
                     SVSYKQGNKVAFVYVKDQGKIGKASIEITITDDGGTAGGGKDKAVFNEQIDIQAFKNP
                     CANFVEYDTQHWQPRPSQNDFPTPGSYQKICTTNSPIEDKARDFFWMKMYGYIIPSKT
                     EYYNFQGFSNEGFDMFMNLNGGPESDWSKLTSLVEGATSIWTSEAYLLEEGKPYYFEV
                     YGRDIVNTQPLWLKWTSASTPLSFISNENLAPFYDIVKPTVPQNFSIQSIGLSDVLVS
                     WSPSTDNRNNLQGYNVFIDGELVNISPVKETSYKIQGLTPGRTYNVHVVSVDDSENNS
                     YPSQVLTFKTYIADDIPPTVPTNLSSEFVTAFSVKLKWNKSTDAETEVRGYNIYQNNV
                     LVNELVKDTTVLVIGLTEKSNYSFTVEAVDANYNKSPKSAAYNVSTVAFDPNETRDGI
                     RKGRVSIKLNPLTKFEGFGINLNYRKTGLLSANLITFGDFEGSQTDTMNVNNLKYYDK
                     QVFNTGFVKDLTTPYEGKVAAKLSPIAGGYFRCVINTSIDKRYTYLVRFAMKKDASFT
                     GVVNVDLKGMFSGTTNKVAVTPTTSWQMYEVELTTTFEGAEPTWYLDFTSTSNGTYYL
                     DNIQFVEKGSYVEGSKFSKRAMDLLRDFKPAHVRWGAIEANSENFKNNSGVGTGYNFS
                     YADWIELANQLNAKAFITVGVGPKTDFYLSPAATFKKFVEYIAGDATTAGGILRTAEG
                     YGNLLSKNKGLMIELGNEVWGGAAHQAGNFNDYTAYGTWARSVATTMKSTSGFDPNKI
                     KVAYSGRYPGQNYGLHKILLSEDKGEGDVLAISGYMGGNLNLDPAIPLKSSLLDYHKA
                     SIKMLQTNLEGLKSEWREMLATCNRLLPMYMYEGNMTTDSYNGSLGQAVTFADYYTTV
                     PLYGVPDVSVFSFESGQWRIIEDRISLKPLPLFTIGKYINKYCTGSMLETEFTTSYKI
                     KDEKGIDLKIEPVGARAYTDSTSYSIALFSRDFENDYQVQVDIPDFIGNSSICKMIRI
                     SGTNFDDREAIVEERIISDFADSLIITVPKYSVVILHFEGNDQHFTPPAVYQSYKKVT
                     SVKITQAENKYYIDTKRGKLALSALVLPEDAFSKIVKWTLLDNETVKAYFVESGSGIM
                     LYGSGVSSGNGTIRVVCAATDLSGKADTVSIVISNQGPSALDSFEAEIVEMHPNPAKD
                     YLRVTMPQSQAGQFFVLDLSGKIHIRQTITGNEQVLNIKSLKPGMYLLRISTLNGTKT
                     LKFIKQESPT"
     gene            complement(5000..7819)
                     /locus_tag="PLM04_02045"
     CDS             complement(5000..7819)
                     /locus_tag="PLM04_02045"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HON01895.1"
                     /translation="MQTRILILLLFLAQLNTLFPMSKIQTVEPSYVWIDQTGEGRNVY
                     AYFRNEFQLIEVPQNAIIYLFAKSLYNLFVNGTFVNFGPIRSYETQPYYDSINIAPYL
                     KTGQNVIAVKVHSIGIHTYQIPMGTAGFIAWGNIQDKSNTVSLATPGNWICRKALGYN
                     PFSPRFSFAQGPVEILDNLAEPDNFKAEKIKPDKWMKPVLLKNQSYFGKLRPRTIPLL
                     TNDAILPKFVVNRLNLSSDECVYNISSYYENTENPSDLRAILCYSYIYSNEEKDVNFG
                     FSWGKYWINGQLIEGVKQPEKPFRSNAKVRLKKGWNSYFSVQEIIWSNLDGMLAVPPD
                     ADLQFSIRRKLNDTIRFALTEPIEKEKINTFVPNDKCPENPEKLKLKWLHTSGTAPML
                     NPAKAIAWLNKNKLPIAVLETENIIIPANTHQAVTYDFGSIHLGRIFLNVEAPAGTVF
                     DLVWSEDLNKDSLVTLYNRFEINAIARFISDGSSYFETFRPFGMRYLQVGVSNHSQPV
                     ILKSAGLKSQIYDFEKTGSFSCSDPLMNDIWEMGWRTLRVCAEDTYIDTPFRERGHYV
                     GDLFPEFATTLAVSGDPRLAKQSIRMFNQQYSKTYSDLAPTIHSDYPILNIIIACWYI
                     RQFNDLDFAREVYPIYANYLRKLHKSRQKDDFYRLDHVFLEWINIDKSADLTAIQSYV
                     YAASDNLSHIANMLGQLADASEFKSISNEIKEIILTKCWDEKSGNFVDGFKNNKVLPT
                     RHVISSSLASVWKIPSMRQEEQIQKWFNDAVINIGEPVNSNQLTTPYGGFYVLASLYQ
                     AGNVCTAEKFIRKHWGKMVIEGNDLTWEDFNRDGKSTISHAWSSSPTYYMSSQVLGVD
                     LGFPGYLSPDTIYIRPQSESVLWAKGSVPHPKGLVTVHWQIQGDILFLRYSAPAGVPV
                     KIEPRGRLASYKLKLTCLTGTGDKL"
     gene            complement(8093..8698)
                     /locus_tag="PLM04_02050"
     CDS             complement(8093..8698)
                     /locus_tag="PLM04_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01896.1"
                     /translation="MHKKVFLFLVMLINAFALYPKTIYIPSSGDNSNGSSSNLIFDAF
                     GVQNLPKPVVSGSNVNTGWSTYKNNILISGNISKSSNLFVNGKLMLVARWPNSGWINI
                     DSITELNGSLTLTCSKLAAHSAVAYNYWKVATMRWRRWSWWFETPTITASFSPDKLKI
                     PGSVAEDSADGWKFYIDNKFEELYTIGEWIYSKGKFMLYRR"
     gene            9835..11649
                     /locus_tag="PLM04_02055"
     CDS             9835..11649
                     /locus_tag="PLM04_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON01897.1"
                     /translation="MKTNWHHLIFVALLCIAASVFSVKAQGKYTATDFHQHTTYSDGS
                     YTFGYMMEKNNQFGLDWWVNSEHGGGFDRWGFASGADLGTEVSWSEAGIEKLGKANGE
                     KMWRWQSLRDWSFRDIMLYRKVYPNKTIIQGFEWNAPGHEHVNVCIINNQFDENTENC
                     LPIAEFEYKFDNNDSDNSNPNGWTKSTNTGKAKTLEAIQWLQTNYPNTSWVIPTHPER
                     ANTFKIEDFRNMNNAGPSVCFGFDSQPGHQKEPNRGGYRTTSYGANGEGATWGGTGYF
                     AAKVGGVWDALLSEERKWWLFANSDCHFNDGDFFPGEYQKTKVFVSNPKNPQSLVDGL
                     RSGNSYVVMGDLIDSLSFTVGSAKMGETFVTADNNVVVSIIVHDPQVDNIHNTYSALK
                     NPELDHIDLIAGKVTSLIDPQSPDYKVDSVTTTSVIARFGKSVHTDANGITTIPWEDL
                     GNGYFKITYTVNLNNQNMYFRLRGTHHAFGDANAEIDDKGNPLVDPFGQNTAEKAFND
                     LWFYSNPIFVTKSNQAGISSPKLENIHIYPNPAKDMLLIQFTEPQTGLIKFVDMSGKC
                     VLQSEIKNSVQEQISCSSLPKGLYFVKINNLMQKVILE"
     gene            complement(12598..13263)
                     /gene="lipB"
                     /locus_tag="PLM04_02060"
     CDS             complement(12598..13263)
                     /gene="lipB"
                     /locus_tag="PLM04_02060"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HON01898.1"
                     /translation="MTRQIEFLDWGLTEYADAWKRQEDLFQLLLQNKKNADFNTHYVV
                     FCQHFPVYTLGKNGHEENLLLNQIQLQTRGASFFRTNRGGDITFHGPGQVVGYFIFDL
                     EKLQLGLKVFIYKLEEVIIHLLEHYGLKGTRLPGATGVWLDAETPRARKICAIGLRSS
                     HFVTMHGFALNVNTDLNYFNYIHPCGFVDKGVTSLQKELFQTVEEEEVKNLLKKQIEK
                     EFL"
     gene            complement(13266..13988)
                     /gene="mtgA"
                     /locus_tag="PLM04_02065"
     CDS             complement(13266..13988)
                     /gene="mtgA"
                     /locus_tag="PLM04_02065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HON01899.1"
                     /translation="MFRKAFKFIKNFIVLFFLISIGWVIIYRFVPVMVTPLMLIRTAE
                     SVFSGETPKISKKWVPIDKISHNMIQAAVAAEDDQFLHHHGFSFEAINQAIEHNKRSR
                     RIRGGSTISQQTAKNVFLWPQRSYIRKGLEAYFTVLIELFWSKERIMEVYLNVIEMGD
                     GIYGVEAASMEYFGKHASKLTKSEAALIAACLPNPRRFNPAHPSGYVLRRQGRIMAMM
                     NKIEPVHFERPEFKFLKHKKQK"
     gene            complement(14003..14461)
                     /locus_tag="PLM04_02070"
     CDS             complement(14003..14461)
                     /locus_tag="PLM04_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01900.1"
                     /translation="MENQINGPKKQYFEPMHTCEHILNQTMIRMFGCGRSQNAHIERK
                     KSKCDYLLPQAPTEEQIRELSDRVNAVIDSHLPVTIEFLKREEASGVVDLSKLPDDAS
                     DVLRIVRVGDYDACACIGQHVANTSEIGHFEIISHDYEEGRWRVRFRLID"
     gene            complement(14741..17026)
                     /locus_tag="PLM04_02075"
     CDS             complement(14741..17026)
                     /locus_tag="PLM04_02075"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HON01901.1"
                     /translation="MKTEKFDVVGMSCAACAAHIEKEVGKLEGVKKVNVNLLTNSMVV
                     DFEESNVDTDKIEEAVVEAGYEAHVKSSAEQAASRETKKIDFVQKEQQEMQKRWWITF
                     GFWLPLLYLSMGSMLGWYIPAFFRGEENAMVFAFTQFLLTLPIAISNEKYFRNGFKML
                     FKAAPNMDSLIAIGSSAAILYGIYGLYKIAYGLGHAQPELVHQFAHDMYFESGATILT
                     LITLGKYLESRSKSQTSRAITQLMDLSPKTATIIRDGTEVEVPAEEVRVGDIIVVRPG
                     QRIPVDGVVESGSSTVDESALTGESIPVLKQSGDRVLSASINKSGYITLKATQVGNDT
                     TLSHIISLVEEASSSKAPISKLADRISRIFVPVVIFIALTAGIVWFSLGYSFDFSLSV
                     AISVLVISCPCALGLATPVAIMVGTGKGAEHGILIKSAASLEMAHKIDTVVLDKTGTL
                     TMGKPQLTDIIPSPNITENELLQIAASLEKLSEHPLAEAILEETDKRKLETFSVKNFT
                     SLPGKGIVAEAGENYFLIGNRQLMEEQKIDVQNFDKIATQLAEEGKTPLFVASREKVI
                     GVIAAADPLKPTSKEAVNQFRSLGLEVVMLTGDHKKTAAAVQRKLGISTVFSEVLPQD
                     KDKVIVNLQKEGKKVAMVGDGINDAPALMRADLGIAIGAGTDIAIESADVVLMRNDLM
                     DAVSAIRLSNAVIRNIKENLFWAFFYNIIGIPLAAGIFYPLLGWKLSPIFAAAAMSFS
                     SVTVVLNALRLRFFKPSEVKS"
     gene            17247..>17806
                     /locus_tag="PLM04_02080"
     CDS             17247..>17806
                     /locus_tag="PLM04_02080"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HON01902.1"
                     /translation="MKKLVLLVFSLILFSGIGFAQNKSDESSHPSLPAALENLTQREK
                     DSILLNKLSPEQLLELKKAELENERKKIEANAREDMPFTGFQLFLICVLPFLFVIILI
                     WIIYAAKNKESKRKFELYMKALEMGQPIPEDFFPKDKKDKPNSNLKKGVLWAAVGLAL
                     LIYFIITHNNDGLIVGIVPTFVGIGY"
     assembly_gap    17807..17906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17925..18470
                     /locus_tag="PLM04_02085"
     CDS             17925..18470
                     /locus_tag="PLM04_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HON01903.1"
                     /translation="MSNRDDLHWITQFTLLGDNKAFEKLVGKYQSPIRRFFLNLTMGD
                     ASLSDDLAQETFIKAFVNIHSFQALSNFSTWLYRIAYNVFYDSMRTQKQFSELDVREI
                     DLQYQSDNAFSAEKLDFYNALQHLKKEEQTVLILHYMEDKTQKEIAKIMNCPEGTVKT
                     YLLKGKNKLMDYLNKEGYDKK"
     gene            18457..18783
                     /locus_tag="PLM04_02090"
     CDS             18457..18783
                     /locus_tag="PLM04_02090"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HON01904.1"
                     /translation="MTKNEQTEEKLNEFFKTHKTEIPDNGFSLKVMRRLPTEKDHSWI
                     VWLIGATGISFLLYHYIHTGILLNFIENLLVMHFDMLLISIAVFPIVCGIVMLLWGKK
                     EKTFFG"
     gene            complement(18869..19219)
                     /gene="rplS"
                     /locus_tag="PLM04_02095"
     CDS             complement(18869..19219)
                     /gene="rplS"
                     /locus_tag="PLM04_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HON01905.1"
                     /translation="MDLLKIAEQSFEQEVKTYPEFRSGDTITVAYRIKEGNKERIQEF
                     KGDVIKISGHGGKKHFTVRKMSGNVGVERIFPMDSPFIESITVNKHGKVRRAKLYYLR
                     NLTGKKAKIKERRV"
     gene            19503..21554
                     /locus_tag="PLM04_02100"
     CDS             19503..21554
                     /locus_tag="PLM04_02100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HON01906.1"
                     /translation="MAIAVLGAGCTEKENHSVIGKQIPNIENGRMTPEVLWSFGRVGG
                     VQISPDRTKILYSVTYYSIPENKGNTELFIMNVDGTNKHQITHTATKEAEARWMKDGE
                     HIAFLSSESGSNQLWTMKIDGSERKQISNREGGINGFLFSPDESKVLFIADVKYGKTA
                     ADKYPDLPKATGRIVTDLMYKHWDEWVESIPHPFIADFKDNTLKNEQDIMKGEPYECP
                     MKPFGGIEQLDWSPDGKIIAYTCRKKTGKEYALSTNSDIYFYYLENGKTENKTEGMQG
                     YDINPKFSPDGKWMAWESMERDGYESDKNRLFVMNVQSGEKLDLSEDFDQNCNAISWS
                     ADSRSIYFVSCWHAVTQIYKTDLRTKSIAPITHGVHDYEFVAEARNGFIGVKHSMSKP
                     DEIYFIDPVTGKEAELSFENKELLSKLKMGKVEERWITTTDKKQMQTWVIYPPDFDPA
                     KKYPALLFCGGGPQSTVSQFWSYRWNFQLMAANGYIIVAPNRRGVPGFGKAWLEQISG
                     DYGGQNMKDYLSAIDALAKEPFVNKNKLGCVGASYGGFSVYWLAGHHNKRFKAFIAHD
                     GMFNLEQQYLETDELWFVNWDLGGPYWDRKNPIVRRTYANSPHLFVDKWDTPILIIHG
                     EKDYRILASQAMAAFNAAVLQGVPAEMLIFPDENHWVLQPQNGILWQRTFFEWLDKWL
                     K"
     gene            21745..22743
                     /gene="pta"
                     /locus_tag="PLM04_02105"
     CDS             21745..22743
                     /gene="pta"
                     /locus_tag="PLM04_02105"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HON01907.1"
                     /translation="MDLLNKILERAKANIKRIVLPEGDETRTLQAAEIILKEKAAHLI
                     LLGKEDVILKTASNLHLNHIAEAEIIDPETSPRKAEYIDLLYNIRKNKGITYQEAEEL
                     TKNPLYFACLMIKNGDADGELAGARNFTGDVLRPAFQIIKTLPGIQVVSGAILMFTKT
                     PQYGENGLLVFADCAVNPNPTAEELAQIAVCTAKTTRQIGEIEPRIAMLSFSTKGSAS
                     HELVDKVVKATRLAREMEPTLLIDGELQADAALVPSVAHRKAPESAVAGKANVLIFPD
                     LQSGNIGYKLVERFGGAEAVGPILQGMAAPVNDLSRGCSVEDIFHMITITSNLAIK"
     gene            22770..23726
                     /locus_tag="PLM04_02110"
     CDS             22770..23726
                     /locus_tag="PLM04_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HON01908.1"
                     /translation="MSQPIIEPIEKYGLSKRSRRRTLFSKIGVVGCGNEGSAIATIAL
                     LNGIEVVFLEPTEEKIANAYSRIERKLNKRIQNWGLTENEKKIALSRVHGTTKYKDFA
                     GCDFVIEAVRYDDQTGERHVEERKEVFLQLEKVLSPQAIIASNISTVVITELASELQY
                     KERCIGLHFLSNVPGSNIIEIVRGLYTSDETYEKICQFARLINHQYVAVAESAGLVSI
                     RLFLIELNEACAMLMEGISNVEDIDKVLTVGFGHRLGVFRTADQMGIEKIVKLMENLY
                     QEYGNSKYKPSPVLLRLSRAKHFGIKKGKGFYEYDEFGNVIK"
     gene            23739..24950
                     /locus_tag="PLM04_02115"
     CDS             23739..24950
                     /locus_tag="PLM04_02115"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HON01909.1"
                     /translation="MKILVLNCGSSSVKYRLLDLENNKVLGSGGVEKIGMKGSLLKHI
                     RQDNQIIILEGEVLEHQTAIEYILGVLTSAKHGCIKSLEEIDAVGHRVVHGAEKFNSS
                     VLITDEVIKKIEECIDIAPLHNPPNLAGINAIGELLPTVPQVAVFDTAFHQTMPEYAY
                     MYAIPYSLYTKYGIRRYGFHGTSHRYVSHRACEFLNMDYVQTKIITVHLGNGASITAI
                     ENGKSVDTSMGFTPLEGLMMGTRSGDVDLGIATFLMEKEMIGPSALSTLFNKHSGVLG
                     VSGISSDMRDIETAIANGNERAKLALDMYEYRIKKYIGAYAAALNGVDLIVFTGGIGE
                     NQAKTRQNIIKGLTYLGIDVDEKANAEARGKEALISSAESRVKVAVIPTDEEFMIASD
                     TMKIINEMKRG"
     gene            complement(25420..27648)
                     /locus_tag="PLM04_02120"
     CDS             complement(25420..27648)
                     /locus_tag="PLM04_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HON01910.1"
                     /translation="MKNHLLSILLLVACHQSIYASSYQSFVKNSEALIQRVLPEKANH
                     FVIEQISSEHGKDVFEIATSKNGQVVLRGNNELSVAMAFNRYLRDVAHVSYDWFSVSP
                     LQIKGELPLPISTIRMACNAQERFFNNTCTFGYTFPFWNWERWEKFIDWLAMNGVNRP
                     LMLAGQEKVWLNVWQSFGLKKEDIQAYFSGPAHLPWHRMANLDKWGGPLPVSYIEGQY
                     RLQKKILARCRELSMKPVLPAFAGHVPEQLKQIYPEANIHQISPGWGGMDADYTTYFL
                     DPSEKLFSEIQYRFLKEQEKLYGISHRYSADPFNEIDPPSWAPEYIASVAKTIYRSMK
                     NADPEAIWYQMSWTFYYDSEHWTQERLKAMVESVPKGKLIFLDYVAEEEEFYKRTENF
                     FGAPFIWCYLGNFGGNTHLVAPMKKVSKRIVEALKVANCLGVGATLEGLNVNPEIYEM
                     VLEIPCSNQISSFNLDEWIKNYAVRRAGQEDSAVIEAWKLLKEKVLVDEAQVIGNHSV
                     VFQVLPVMDLKESSWTTNPFVPYDNPDLAKALQIMLQANEESRKNDAYCFDVVNLARQ
                     ALGNYGSCLYEKMMTAYREQNLSDFQKYSHQFISLGMEIDLLLGTRKEFLLGSWLSDA
                     RRWGKTREEKIYYERNAREIITTWHKSGGELVDYANRQWNGLMRSYYLPRWEKFIELL
                     QKSLINGTSFEQEDFSAWCIDFERKWVNGEIQENFLEKPSGNAIETAQRLFEKYSTEM
                     LK"
     gene            27835..28653
                     /gene="nagB"
                     /locus_tag="PLM04_02125"
     CDS             27835..28653
                     /gene="nagB"
                     /locus_tag="PLM04_02125"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HON01911.1"
                     /translation="MRLIIQPDYPTLSQWAANYVAAKILHAQPSAQKPFVLGLPTGSS
                     PLGMYHYLIELNKKGIISFENVITFNMDEYVGLPREHEESYYSFMWKNFFSHIDIKPE
                     NVNILNGNAEDLIAECAAYEEKMKSVGGIELFVGGIGPDGHIAFNEPGSSLSSRTRQK
                     TLTTDTILANSRFFNNDINQVPKTALTVGVGTIMDAREVLILVNGHNKARALRHAVEE
                     PISQMWTVSALQLHPKSIIVCDYDACIELKVGTYKYFLDIEKDYLNPESFNIFP"
     gene            28693..29232
                     /locus_tag="PLM04_02130"
     CDS             28693..29232
                     /locus_tag="PLM04_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON01912.1"
                     /translation="MIDFKRMQSSSDAFFQQFYRLYSSAFPENERRSLQNLEKELNNP
                     NFHAYVLMNKNRFVGIFNYWNFEKFIYIEHFAIEEKLRSQYLGTEALKSFLEKVNLPV
                     ILEVEMPNTDAAKRRIEFYHRHEFVELQNYYLQPPYESGGFPIPMQLMSNDYEFAKNN
                     FETIKQTIYLEVYHFKPEV"
     gene            29446..30522
                     /locus_tag="PLM04_02135"
     CDS             29446..30522
                     /locus_tag="PLM04_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HON01913.1"
                     /translation="MKKTVSIIFMMLLFVSASNAQKAVIQFQEKIHDFGQIKEEDGKV
                     SYQFKFTNTGTGPLVISRVQASCGCTTPNWTKEPIEPGKSGSIMVTYNPLGRPGIFNK
                     TITVFSNATEEQVQLQIKGEVIPKTDENSDYPISVSGIRLKTKIVQMNNVNKGTIQSR
                     SISFKNTTSQNVRITFENLPSYVTSEVIPDITQPNEEGKITFYLNSKNCQQWGPISDD
                     FYLIVNGQKKFSEDNKLTLYANIVEDFSKLSVSERQNAPILDIPSTTLSLGTFKAGSK
                     ISGKFLIKNIGKDELKIHRIINNNKELTVSSVQTIKGGSKGELKVTVNTANLASGNYK
                     KTITLQTNDPNNSFVILVVNWTIS"
     gene            30604..31713
                     /gene="meaB"
                     /locus_tag="PLM04_02140"
     CDS             30604..31713
                     /gene="meaB"
                     /locus_tag="PLM04_02140"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HON01914.1"
                     /translation="MHFHQKHPENDPSFKNLSVNDGVNKPPIVNPYQKVKQKRKIYSA
                     SEYVKGILSGDISLLSQAVTLVESSLTEHQNIAQEIIEKCLPYSGKSVRLGITGVPGA
                     GKSTFIEALGMYLVQKGHRLAVLAIDPSSERTKGSILGDKTRMEELSVSKNAFIRPSP
                     SAGTLGGVARKTRESIILCEAAGFDRVFVETVGVGQSETAVHSMVDFFLLLQIAGAGD
                     ELQGIKRGIMEMADGIVINKCDGNNIEKAQLAKIQHQNALQLFPPAESGWKPEVLTCS
                     AIEKTGIEDVWNMVERYVEYTKANGFFEYKRNDQAKYWMYETINEQLKAHFYQNEEIR
                     KLLPEIENKILTNELTSFVAAKKLLETYFGKINKK"
     gene            31744..32457
                     /locus_tag="PLM04_02145"
     CDS             31744..32457
                     /locus_tag="PLM04_02145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON01915.1"
                     /translation="MEMNQIYALSCLEGLKKLPDHSIDLIFTDPPYYQYRAQNIKGLK
                     NHKDVVTEFEFDDFKSEEEYLQFLEEVLKECYRVSKPGASGYLWCGDDFVSFLNRMVE
                     RAGFQFRKVIHWHKTNPFPAVHPRKMFANSMELIVHFSKGTPKTWNYKSTNEMHNFIE
                     APICMGKERTIHRTQKPLKVCIPYIEISSNEGDLVLDPFMGSGTTAVACKMLKRNFIG
                     FEINPLYCEIANQRLQKVE"
     gene            complement(32482..32667)
                     /locus_tag="PLM04_02150"
     CDS             complement(32482..32667)
                     /locus_tag="PLM04_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01916.1"
                     /translation="MKQAKWWTLQQIEENLQEGIFTENFLIEFDLLKKSGVLENNICD
                     CACKLKETIFKAPHLFR"
     gene            complement(32807..33310)
                     /locus_tag="PLM04_02155"
     CDS             complement(32807..33310)
                     /locus_tag="PLM04_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HON01917.1"
                     /translation="MAQHQIDRLDRKILQLISQDARIPFLEVARECNVSGAAIHQRIQ
                     KLRNLGIIKGSEFVIDTYKVGFQTCAYIGIILSDIKMFKSVVEELKKIPEIVECHYAT
                     GKYSMFIKIYAKDNRHLLQIILEKLTVIPGVAHTETFQISLDEIFRRQLFVFDTENEV
                     ENLEEEI"
     gene            complement(33510..34418)
                     /locus_tag="PLM04_02160"
     CDS             complement(33510..34418)
                     /locus_tag="PLM04_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HON01918.1"
                     /translation="MKIRFLIVIFLFAVIVSMFGQSNIRVNNYWENTYYINPASIHDQ
                     YNGEISMAARNQWINFPGSPTTFFGHGTVFLSDFHTQIGMKAMADKIGYTNTYDLAFS
                     YAYQLTLNPTWNLSMGLTVNYQGLNYDLSQVNLTNGDDPSAYEQFKFESNFNSNLGVE
                     LNNKYWKIGLAGQNIFSLFYSFSELFNNTNFLYASYKPYTRNFFSVGGGMCLIQYGNM
                     MQAELNFNGFFKMTPENNAFQLGLFYRTWHEAGALFGIDLTNSFRLSYSYDYNAGAIR
                     RNSYGTHELILTYKIKGEPKCINCWY"
     gene            complement(34429..40992)
                     /locus_tag="PLM04_02165"
     CDS             complement(34429..40992)
                     /locus_tag="PLM04_02165"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HON01919.1"
                     /translation="MKKYLRVFLALTEMIFFSSYSFAAFHFTDPNENFLYQKNIFSFY
                     PVNNGNTLSATINADKLGVCINGTSPIITFTATNGTAPYVFTYKINNGALQTITSESG
                     KNTASIHVPTNTAGIFNYVLVSISDATPITQNISNQQVTVTVGNIPSVDFTFSDENCA
                     GKNITFTPINLPAGADYSFMWEFGDNTTSTDSVAHHIFMTAIGNGTQTFTVKLTVTDN
                     LTACTNSKQKTITLSQHPDPKLETSAESDTLNGQVTFMSCSKVATEFVFTNPNENNPY
                     IVNYTIDWGDGSPIFSESTWSLLTHTYNLGMNTLKYTVQGQNGCSVTKEYKIFVGSNP
                     AGGILSPGNTDICVNEQLTFPIAGIQNNPPGTIYMVSFNDGTPEEVFYDNTRTQITHT
                     FTNGSCGTYSYDGRNYYPNSFMVSMTAKNPCSPSGTSSAVTPIYVSIPPEANFNANNS
                     TCVNTDFFIQNTSRRGYVASSSGCTSGDKFVWSISPTEGVTVVSGSLGNTRGLNDEQY
                     SSWISGSDNVILRFANPGNYTIKIKIANRCGASDTTQTICVEPVVTPSFTLDKNKGCA
                     PFTVKVNNTTDTTKLCSNPSYNWTVGYQPANCGTTSSFDYVDHTSTSSKNPHITFNNP
                     GIYTLTMNVYTNCSVQTVSQQVTVTRPPLVSIGNIPDFCGSAEISPIATIQTCIPATD
                     SVSYEWSFAGGTPASSTAASPGKITYSSPGNYTVSLTVKNGCGSTVATKTFKINVVPQ
                     ITNTELSQTICSGDSSQSVPLTSTVPGTTFSWTAQASTGITGFISSGTSNTIPAQNII
                     TSANVSGNVTYSIVPKVGTCTGDTVRYVINVYPRPYFTKQPVSSILCKDGAATLLSVS
                     FSKGEGTFSFQWYSNNVKSTSGGTPIPGAVDSVYTPSTSTVGTTYYYCIIEFSSGYCR
                     EIISDIATVTVNEPPQITQQPLASQKICVGGSLPQPLKVKYSGGAGPSFQWYSNNVDS
                     NVGGTAISGANDSIFTSSSFTATGTFYFYVTITFSGNNCDPVTSETAKVEVIPDPSIA
                     QQPLASQTICQNSIAEELSVKVTGGIGKYAYQWYQNTTNSNSGGSIIPNANDSIYMPS
                     TTNTGTLYYYCEITQPNGPGCNAVSNTSQVIVKEGPAITEQPSSLVVCKNEISPKLVV
                     SWKNGVGIPSYQWFSNTLNSNTGGTLIPTATDSVYMPSTAVVGTIYYYCVISFSSGGC
                     SELVSNPAAVTVNQFPVISDINVSICSGQSFNIIPENSGSNIVPTGTTYTWSMPAISP
                     SNSITGASAQSNPQPQVSQTLTNITTSIAIVTYTVKPSANGCDGAEFKISVTVYPAAH
                     IEANVHNITCNGANDGMISLTITGGVPFATGDPYQITWNGPNGFTSHNANISNLSAGD
                     YQLQVLDAGGCPYQATYTIIEPEKIKISGITQKNVSCYGNADGEISFNVSGGTGNYVY
                     SWTKDGTTFTGTNHLTALQAGTYAVSVTDGNACAPQSEEFTIIEPLPLTVSLSDKKNV
                     LCYGDATGSIDVEVTGGTKAEITSGDYDYYYSWTGPENYTSNNKNLTNLKAGDYFLTV
                     RDASNCTVTSDAFTVTQPQELIVSATSTAITCFGDDNASITLNVTGGVEPYSVQWSNY
                     GEGMVQENLSPGTYDITVTDNNGCQKSLQVIIPEPFRFSVKPVVKQISCYGANDGSIK
                     LNFVGGMPPISFAWDDDPNAGVDRNQLKPGVYTVRIHDAQPCDIIRSFTIIEPQPLTV
                     SAQVKDATDCQNVNSGSILLAVTGGRPPYKYEWSNGMTTQNILNVPPGNYSVLVTDSF
                     GCNVTVQHSIQRQLPLYVQVNSDIEFDCLTKNIIQVNQATVTGGVPPYQISWSSGTIS
                     GANNEIMTTGQNGLVFVHVTDALQCEITTSINIQIPEQGISYQLLDCNQRSYQFNALI
                     PIEKADYIYSWDFGDGGVSDIKNPQYNFSKPGNYTVKLKVQNAECSLYYEKTISVEAP
                     PVLSLDKEPIFCIGDSLLLHVSGADYYRWNDGSTADNLLIVKPGTYSVEGTSKAGCSS
                     ILTFMASYFDLFHYTIQTDQEMVTDRQPTIEFWSEDTSNAIYYWDFGDGYKTQGNHVS
                     HSYIISRDGYYEVNLQVINPNGCREYATKRIWIGAPTLPNTITPNGDGKNDLLLKGFH
                     IQVFNRNGLLLFDGREGWDATYKGKPVSEGTYFYVLMYATPKGLKFASNFVTVIR"
     gene            complement(41122..42060)
                     /gene="rsgA"
                     /locus_tag="PLM04_02170"
     CDS             complement(41122..42060)
                     /gene="rsgA"
                     /locus_tag="PLM04_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HON01920.1"
                     /translation="MLGTVVKSTGSWYLVETEEKERVECKIKGNFRLKGIRSTNPIAV
                     GDRVEFEKNEDGIGLINRIYDRKNYIIRRSSNLSKEFSIMAANLDIAALIITLNYPET
                     STVFIDRFLAVTEAYNVPACLIINKTDLYGKEDVEYMEALAYLYENIGYPVFKISVLK
                     GENLSAIKEFFTGKTVLLAGNSGVGKSSLLNALSPEARAKTADISFYHNKGMHTTTYS
                     EMYRLPDGGYIIDTPGIKGFGLIDMEESEVDHYFKEIFKISSQCKYDNCLHIQEPECA
                     VREAVEKGEISWSRYQSYLSVLEDCHAEKYRKDEYH"
     gene            complement(42208..42768)
                     /gene="frr"
                     /locus_tag="PLM04_02175"
     CDS             complement(42208..42768)
                     /gene="frr"
                     /locus_tag="PLM04_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HON01921.1"
                     /translation="MQDVKPILDEAKESMETTIQFLENALAHIRAGKANPRILDAVRV
                     DYYGVHVPLANVASITTPDAKTILIQPWEKAMLKPIEKAILNSDLGINPTDNGEVIRL
                     SMPPLTEERRKQLVKQVRMEVEEARIRIRNARRNAIDHIKKMIKEGLPEDVEKDAEEQ
                     VQKLHDDYIKIIDEMLALKEKEIMTV"
     gene            complement(42879..43589)
                     /gene="pyrH"
                     /locus_tag="PLM04_02180"
     CDS             complement(42879..43589)
                     /gene="pyrH"
                     /locus_tag="PLM04_02180"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HON01922.1"
                     /translation="MLYYRRILLKLSGESLMGGKQYGLDENRLQEYAAQIAEVAKMGV
                     QVGIVIGGGNIFRGLSGTKKGFDRILGDQMGMLATVINSLALHSALQAEGATSHVLTA
                     IRMEPVGEYYSKQKAVSSLEKGEIVIISGGTGNPFFTTDTASSLRAIEIGADIMLKGT
                     RVDGVYTADPEKNSDAERFEEITYDEVYRRNLKIMDLTATTLCKENNMPIYVFNMDTI
                     GNLKKVILGEKIGTLIKN"
     gene            complement(43728..44018)
                     /locus_tag="PLM04_02185"
     CDS             complement(43728..44018)
                     /locus_tag="PLM04_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HON01923.1"
                     /translation="MINFNGLLVGFAVFLLIGIFHPIVIKTEYFWGVRSWWIFFIIGS
                     IAAVVSLFVEKEIPSILLGVFAFSCFWSIREIFQQKRRVEKGWFPKNPKRKK"
BASE COUNT        13709 a         8603 c         8254 g        13535 t
ORIGIN      
        1 aaaaaaaata atcaataaga attccccgat gcttgcgtcg gggtagcttt tgtttttatt
       61 tttttggaaa tttcagtggc gaagccataa aattggctta atacctcgcc gcaagcaagg
      121 aggtattaag ccaattttat agaatttctt tatttctctt ttttgaagaa aatcaggttt
      181 taagtggggg attcttgctt tataaatttc aatgtttttg ttccattaag tgttgaaatg
      241 cgaagcaaat acataccagg ttttagactt ttgatattca acacttgctc attgccggtt
      301 atggtctgtc ggatatgtat ttttccactc aaatctagaa caaaaaactg tccagcctga
      361 ctttggggca tggtcacccg taaataatct ttggccggat taggatgcat ttccacaatt
      421 tcagcttcga aactgtcgag agctgaaggt ccctgattgg aaataacaat actgacagtg
      481 tcggcttttc ctgataaatc ggtagccgca cataccactc taatggttcc gtttccggag
      541 cttacgcctg aaccataaag cattatacca cttccacttt caacgaaata agctttaaca
      601 gtttcattat caagtaatgt ccattttact atcttgctaa atgcatcctc aggtaaaacc
      661 aaagcactaa gtgccaattt tcccctttta gtatcaatat aatacttgtt ttcagcttgt
      721 gtaattttta cagatgttac ctttttgtag ctctgatata cagcaggtgg ggtaaagtgc
      781 tgatcgttac cttcaaaatg gagaataaca acactgtatt taggcacagt aataataaga
      841 ctgtcggcaa agtctgaaat tattcgttcc tcaactattg cttctctgtc atcaaagtta
      901 gttcccgaaa ttcgtatcat tttgcaaatg gaagaatttc caataaagtc tggaatatcc
      961 acctgaacct gataatcatt ttcaaaatcg cgactgaaaa gtgcaatcga ataacttgta
     1021 ctatcggtat aagcccttgc acctaccggt tcgatcttca gatcaatccc cttttcatct
     1081 ttaattttat aggaagtagt gaattcagtt tcgagcatgg aaccggtaca gtacttatta
     1141 atatactttc cgatggtaaa taatggaaga ggtttcagag aaatacggtc ttcgataatt
     1201 cgccattgtc cactctcgaa ggaaaatact gacacatcgg gcacaccata caatggtacc
     1261 gtagtataat agtcggcaaa tgtaacagct tgacccaacg acccgttgta tgaatcagta
     1321 gtcatatttc cctcatacat atacattgga agtagtctat tgcaggtagc cagcatttcg
     1381 cgccattcac ttttaagacc ttccagattg gtttgtaaca ttttaatgga agccttgtgg
     1441 tagtctaata gggatgattt aagcggaata gcaggatcca gattaagatt tcctcccata
     1501 taaccactaa ttgcaagtac gtctccctca cctttatctt cggaaagcag aattttatgc
     1561 aatccataat tttgacccgg atagcggcca ctgtatgcca cttttatttt attggggtca
     1621 aaaccggatg tcgacttcat ggtagttgct actgagcgcg cccaggttcc gtaagcagta
     1681 taatcgttga aattacctgc ctgatgcgca gctccacccc aaacttcgtt accaagttca
     1741 atcatcaatc ctttgttttt tgaaagtaag ttgccgtaac cttctgcagt ccgtaatata
     1801 cctccggcag tagttgcatc tccggctata tattccacaa attttttgaa cgtggcagca
     1861 ggcgacaggt aaaaatcagt tttcggtcct accccaactg ttataaatgc tttagcatta
     1921 agctggttgg ctaattcaat ccagtcggca tacgaaaaat tatagccagt gcctactccg
     1981 gaattatttt tgaaattttc cgaattagct tctatagctc cccagcgaac atgggcaggt
     2041 ttaaagtcac gaagcaaatc catagcccgt ttcgaaaatt tactaccttc aacatacgat
     2101 cccttttcga caaactgaat attatcaaga taatacgttc cattagaagt ggatgtaaaa
     2161 tctaggtacc aggtaggttc agctccttcg aatgtagtgg tgagttcgac ttcatacatc
     2221 tgccaactcg tagtcggagt gaccgccact ttattggtag tacctgaaaa cattcctttg
     2281 aggtcgacat ttacaactcc tgtaaatgag gcgtccttct tcattgcgaa acgaaccaga
     2341 taagtatatc gtttgtcgat acttgtattg attacacaac ggaaatatcc acctgcaatg
     2401 gggctcagtt tggcagcgac ttttccctcg taaggtgtag ttaaatcctt tacaaaacca
     2461 gtattgaata cttgtttatc atagtacttc agattgttta cattcattgt gtcggtttgt
     2521 gaaccttcaa aatcgccaaa agtaattaaa ttggcactaa gcaaaccagt ttttcggtaa
     2581 ttcagattta tgccaaaacc ttcgaactta gtgagaggat taagttttat actcactcgt
     2641 ccttttcgga taccatcacg tgtttcattg ggatcaaaag ccactgttga cacattatat
     2701 gcagcacttt taggtgattt attataattt gcatccacag cttccactgt aaaactgtaa
     2761 ttgctttttt cagttaatcc tatcactaac acagtagtat ctttaactaa ttcattaaca
     2821 aggacattat tttgataaat attgtagccg cgcacttcgg tttcggcatc agtagatttg
     2881 ttccatttta gcttcaccga aaatgctgta acaaattcgc tgctgagatt tgtaggaaca
     2941 gtaggtggga tatcatcagc gatgtaggtt ttaaaagtca aaacttggga aggatacgaa
     3001 ttattttcag aatcatctac cgaaaccaca tgcacattgt atgtcctgcc gggtgtaagt
     3061 ccctgtattt tgtacgatgt ttcttttacg ggactaatgt taaccaactc tccatcaata
     3121 aacacattat atccctgaag gttgttgcgg ttatcggttg aagggctcca actaacaagt
     3181 acatctgata aaccgatgct ttgaatacta aaattttgcg gaacagttgg ttttacaata
     3241 tcgtaaaacg gtgccagatt ttcgttagaa atgaaactga ggggcgtgct ggcactggtc
     3301 cactttaacc aaagtggctg agtgtttaca atatcgcgcc catatacttc gaaataatac
     3361 ggtttacctt cttcgagcaa ataagcttct gaagtccata tgctggtagc tccttctacc
     3421 agcgaagtta gttttgacca atcactttca ggcccgccgt tcaggttcat aaacatatca
     3481 aaaccctcgt tggaaaatcc ctgaaaatta tagtactctg tttttgatgg aataatataa
     3541 ccatacattt tcatccaaaa aaagtcacga gccttgtctt ctatcggaga gttagttgta
     3601 caaattttct gatacgaacc tggtgtggga aaatcgtttt gcgaagggcg tggttgccag
     3661 tgttgagtat catattccac aaagtttgcg caaggatttt tgaatgcctg aatatcaatc
     3721 tgctcattaa acacagcttt atcttttcct cctccggcag tgccaccatc gtcagtaata
     3781 gtaatctcta tactcgcttt gcctattttt ccctgatcct taacatatac aaatgccact
     3841 ttattacctt gtttgtaact cactgagtcg atggttagta attcaggcgt tgttgaaaca
     3901 actgaaattg taacagtttg ttttttgtct atttcaccat cgctaatttg tggtaaagtg
     3961 ataatttgct gaccacgatt agcatacagt tttgacataa tctgacccat acttggttcg
     4021 ttattaagaa cagggtcaat atatttttgt acaattaacg gtgcgcttgc ctccgaaaca
     4081 ttatttggca ttgcaccatc ttttgcctta attgttacaa taaaagtgtt gcctgagagt
     4141 ccggttacag agtaactagt attttgggta gttcctctta gttttccatc cacgtaaatt
     4201 tcataagtta caaaaccaac tccgccttca ttatcaaccg atggagacca tgtcaggtca
     4261 aaggagcggg aagttacatt cgatgcaact atatttgttg gtactgccgg aggttgatta
     4321 tctattaact gtgcaggaat cggaaactct acagttttct ctgttaaacc actgccaaaa
     4381 gttgaagctt tatcgtaatt ttgtttgtca aggagtaaat accagttaat attgtcattt
     4441 gagccatagc aggaaaatcc cgcgaaccag caatcgggac ggatggttat ttttatactt
     4501 gttggtacta tcggagtagt attatcaagg gttgcccata ttggaaatgg tctttcccga
     4561 agatataccc atgcctgatt aatttttcca tcgtacatat ttatacctga tgagccccat
     4621 tcccccgtag ccgaagaagt taccagcgta gtgttggttg tgaattttgt tatcggatat
     4681 gccgtggtgc caaccatcca ttccacttca ccaacttcta aataattaat tgtcgatgta
     4741 cctgccttat ctacctgaaa tttcagatac ctgaatgctg tattatttgt tggtggaatt
     4801 tgctgggctt gtaatgcagt tacaagcacg ataaacattg actgaaaata aaagcttaat
     4861 cttctcatac tattaattct ttagttagtt gaagcaaaga taattttaca tcctcaaaaa
     4921 aaaacaatac ttttttatcc ataaatgata gttagtttat atcactatca gaacatggat
     4981 ataagtttta cattcaaacc tataatttat cccctgtgcc tgttaagcaa gtaagtttta
     5041 atttatagga agctaatctg cctcgtggtt caatttttac cgggacaccg gcaggagcac
     5101 tgtatctaag aaaaagtata tcgccctgaa tctgccaatg cacagttacc aatccttttg
     5161 ggtgtggtac cgatccctta gcccacaata cggattcact ttgtggacgt atgtatatgg
     5221 tatcgggtga taaataaccc ggaaatccca gatcgacacc taatacctgc gagctcatgt
     5281 aataagttgg tgagcttgac catgcgtggc taattgttga tttcccgtcg cgattgaagt
     5341 cttcccaagt taaatcgttg ccttctatca ccattttacc ccagtgtttg cgaataaatt
     5401 tttcggctgt acaaacattg ccagcctgat aaagagatgc taacacataa aaaccgccat
     5461 aaggtgtggt tagctgattc gagtttaccg gttctccgat attgataacc gcgtcattaa
     5521 accacttttg aatttgctct tcttgcctca tggaaggtat tttccatact gatgccaaag
     5581 aacttgagat aacgtgtctt gtaggaagca ccttattgtt tttaaaacca tccacaaaat
     5641 tgcccgactt ttcgtcccag catttagtca gtataatttc ttttatttca ttcgaaatag
     5701 atttgaattc agaagcatcg gcaagctgtc caagcatatt tgctatatga gataagttgt
     5761 cggaggcagc ataaacatag gattgtatag ccgttaagtc agccgatttg tcaatgttta
     5821 tccattccag aaaaacatga tccagacgat agaaatcatc tttttgcctg ctcttatgca
     5881 attttctcag gtagttggcg taaatgggat aaacttcacg ggcaaaatcc aaatcgttga
     5941 actgacgaat ataccaacaa gcaatgataa tattcagaat cggatagtca gagtgaatcg
     6001 taggtgccaa atccgaataa gttttactgt attgctgatt aaacatgcgt atagattgtt
     6061 ttgctaatcg aggatcgcca ctcactgcta aagttgttgc gaattcgggg aacaaatcgc
     6121 ctacataatg accacgttcg cgaaatggtg tatcgatata cgtatcttct gcacaaacac
     6181 gcagggtacg ccatcccatt tcccatatat cattcattag aggatcggaa cagctaaaac
     6241 ttcctgtctt ttcaaagtcg taaatctgac ttttcaggcc tgcacttttt agaattacag
     6301 gctgtgaatg attgcttaca cctacttgta aatacctcat cccgaaagga cggaaagttt
     6361 cgaaatacga acttccatct gagataaaac gggcaattgc attaatttcg aatcggttat
     6421 ataaagttac aagagagtct ttatttaaat cttcgctcca aactaaatcg aaaactgtac
     6481 ctgcgggagc ctccacattg aggaatattc gccccagatg aattgaccca aaatcgtaag
     6541 taacagcttg atgtgtgttg gcaggaataa taatattttc agtttcgagt acagctatgg
     6601 gcaatttatt cttgttcaac caggctatcg ctttggcagg attcaacata ggagctgtac
     6661 cagatgtatg cagccatttt aatttcagct tttcgggatt ttcggggcac ttatcgttgg
     6721 gaacaaaagt attaattttt tccttttcga tgggttctgt caatgcaaaa cgtatagtat
     6781 cgttaagttt gcgtcgaatc gaaaattgta aatctgcatc gggagggact gcaagcatac
     6841 catccagatt cgaccagata atttcctgta ctgaaaagta actgttccaa ccttttttta
     6901 aacgcacttt tgcattactt ctgaacggtt tttcaggctg ttttacacct tcgataagct
     6961 gtccatttat ccaatactta ccccaactga atccaaagtt cacatctttt tcttcgttag
     7021 aataaatata agaataacat aaaattgcac gcaaatcgga agggttttcg gtgttttcgt
     7081 aatacgaaga tatattatag acacattcgt cggagcttag gttaagtctg ttcaccacaa
     7141 atttcggcaa aattgcatca ttagttaata acggaattgt acggggacgt aattttccaa
     7201 aatagctttg attctttaat aatacgggtt tcatccattt atcaggtttt attttttcag
     7261 ctttaaaatt atcaggttca gccaagttat ctaaaatttc aaccggtccc tgtgcaaagg
     7321 aaaaacgagg tgaaaaaggg ttgtaaccta atgctttcct acaaatccaa ttacctggtg
     7381 ttgccagcga aactgtattg cttttatcct gaatattgcc ccaggcaatg aaacctgctg
     7441 tacccatagg aatctgatag gtatgaatgc cgatactatg aacttttaca gcaattacat
     7501 tttgtcctgt ttttaaatat ggcgcaatat ttatgctatc gtagtatggc tgtgtttcgt
     7561 aactacgaat tggcccgaaa ttaacaaaag tgccattcac aaaaagattg taaagtgatt
     7621 ttgcaaacag ataaatgatt gcattttgtg gcacttctat caattggaat tcattccgga
     7681 aataggcata tacatttcgg ccttcaccag tttgatcaat ccatacataa gaaggttcaa
     7741 ctgtttgaat tttgctcatg ggaaacaacg tattaagttg agccaaaaaa agaagtaata
     7801 ttaaaattct ggtttgcatt ctgatttcta tttttttaat tctttaatat gctttattta
     7861 aaatacattt tgttccttaa taagtctcaa aagagtttca tttttatcaa tgtcaattat
     7921 ccgtattaca attatatatt atcgaaactg caattttcga taacacaggc ttcattgata
     7981 ctcagctcgt ggtcggtttg gtaccggaaa accacatttc aaatggttaa tgcatttagt
     8041 tacaaaccat aactgagcac tgcaccttcg ataaggtatt gatttgttgc gcttaccggc
     8101 ggtataacat aaactttccc ttcgaataaa tccattcgcc gattgtatat aattcttcga
     8161 atttattatc gatataaaat ttccaaccat cggctgaatc ttcagcaaca gaccctggaa
     8221 tttttagctt gtcgggagaa aaggatgctg taattgttgg tgtttcgaac caccagctcc
     8281 atctgcgcca gcgcatagtg gctactttcc agtaattata ggctactgca ctgtgagctg
     8341 caagttttga acaagtgagc gtaaggcttc cgtttagctc agtgatgctg tcaatattta
     8401 tccatccact attgggccaa cgggcaacca acattaactt tccgttcaca aacaaattgg
     8461 aagactttga tatattgccc gatatcagga tattattctt gtaagtactc cagcctgtat
     8521 tgacattact tccactcaca acaggttttg gtagattctg aacgccaaag gcatcgaata
     8581 taagattcga tgatgaacca ttgctattgt cgcctgatga aggtatgtaa atggtttttg
     8641 gataaagcgc gaaagcattt atcaacatta ccaaaaataa aaatactttt ttgtgcattc
     8701 gatttaaaat agattattct tttttttacc acacagaata ctgtaatttg cattttatat
     8761 cagctttaga taaaaatgag acagaaagaa cagcaaagca aatggttgat ttcttattca
     8821 tcgttgtgct cgtaataaaa ttaatttcgg taagtacaaa aatacgtgaa aaagtagagt
     8881 tttaataatc tttttttgac caatattgat atttaagttc catttaccta taaaactcat
     8941 tgaaatatat aaatttatgt atgaaaagta ttaacctatt cgtaacaatg tgcttaattt
     9001 tgtttactgc atcaaaattt tttattttta tttcatccca aggatgtttt tttagattaa
     9061 cttcatgatt ataagttggt tgctgaatga aatgtatatt tccgggcact cttaaacctt
     9121 ttgctttgtt ccatcctgct ccatctatct gcatgataat ttcaaattcc ttaaattcat
     9181 tggatacagg atacattttc taaaaataaa ctcatcattt cggtatttgc atacagtaat
     9241 attaaagaac taatgtggcc taacttcgga catattcctg cataaacata aaaactttct
     9301 cttaccactt gttttgctac cagaggacga acctgtttgg ttgcccaaca acttcttgga
     9361 atatttactc ttccaaatca tctttttggt ttgaatttaa gatacaaatt gttgacttga
     9421 taaacgctat gttttaccaa gtcaacaata atttaataca aaaatggttt tttaaagctc
     9481 gactaattaa caattataga ttacaaattc attgattaac aaagctttgc attagaaatc
     9541 ctcgcttcga tggaagcaga agccacacat gcaacagtta cacgtcatta cggacttcaa
     9601 tagcaaggaa aagaaacttc taccaatcct attacttcca tttttgcttg gacgcgccga
     9661 ctggtaaaaa gcagaaaact aaaaagataa atggaaaaaa ttaactcttt ccgaaacatt
     9721 ctgttcatat caattttcaa acaatgaaat attcgcgtaa ccaattgata acattaccct
     9781 cttacttttg ccatagaatt aaaaacaaat tactaactaa aaaattacat ttaaatgaaa
     9841 acaaattggc atcatttaat tttcgttgca ttgttgtgca tagcagcaag tgtattttca
     9901 gtgaaagcac aaggaaaata tactgcaaca gatttccatc aacatactac ttattctgat
     9961 ggaagttata cgtttggcta catgatggaa aagaacaatc agtttggatt ggattggtgg
    10021 gtgaatagtg aacacggagg tggatttgac agatggggat ttgcatccgg agccgattta
    10081 ggtacggaag taagctggtc ggaagcaggt atcgaaaaat tggggaaagc aaacggagaa
    10141 aagatgtgga gatggcaatc gttgcgcgac tggtcgttca gagatataat gctttacaga
    10201 aaagtatatc caaacaaaac gattattcag ggttttgaat ggaatgcacc cggacatgaa
    10261 catgtaaatg tttgtattat caacaaccag tttgatgaaa acacagagaa ttgtcttcct
    10321 attgccgaat ttgaatacaa atttgacaac aatgattcag ataattcaaa tcccaatggt
    10381 tggacaaaaa gtacaaatac aggaaaagct aaaacattgg aagccattca atggttgcaa
    10441 acgaattatc cgaatacttc atgggttatc cctacacacc ccgaaagagc caatactttc
    10501 aaaattgaag attttcgtaa tatgaacaat gctggtcctt ccgtatgttt tggttttgac
    10561 agtcagcccg gccatcagaa agaacctaat cgaggcggtt atagaactac ttcttatgga
    10621 gctaatggtg aaggagctac atggggagga accggatact ttgctgccaa agtaggaggt
    10681 gtatgggatg ctctgttgag tgaagaacgc aaatggtggt tgtttgccaa ctctgattgt
    10741 cattttaatg atggtgattt ctttccaggt gaatatcaga aaacaaaagt atttgtaagc
    10801 aatcctaaaa atccacaatc gctcgttgat gggcttcgtt cgggtaactc ttatgtggta
    10861 atgggagatt taatcgattc attaagtttt actgttggtt cggcaaaaat gggtgaaact
    10921 tttgttactg cggataacaa tgtggttgtt agtattattg tgcatgatcc tcaagttgat
    10981 aacattcata atacctattc tgctttaaaa aatcctgaac tggatcacat agacttgata
    11041 gctgggaaag taacttcact tattgaccca caaagtcctg attacaaagt cgatagtgta
    11101 acaacaactt ctgtaatcgc acgatttggc aaatcagttc atactgatgc caatggtatc
    11161 acgaccattc cgtgggaaga tcttggaaat ggatatttta aaattactta cacggtaaat
    11221 ttgaataatc aaaacatgta tttccgtttg cgtggaacgc atcacgcttt cggagatgcc
    11281 aatgccgaga tagacgacaa gggaaatcca ctggttgatc cgtttggcca aaatactgcc
    11341 gaaaaagctt ttaacgacct ctggttttac agcaatccta tttttgtaac gaaaagtaat
    11401 caagccggaa tttcttctcc taaattagaa aatatccaca tttatcccaa cccagcaaaa
    11461 gatatgcttt taattcagtt tactgaacct caaacaggcc tcataaagtt tgtagatatg
    11521 tcaggtaagt gtgttctgca atcagaaatc aaaaattcgg ttcaagaaca aatttcttgc
    11581 agttctttgc caaagggatt gtattttgta aaaatcaaca acttaatgca gaaagtaata
    11641 ttggaatgat taattgattt atatgaaacc ctcatgtatt gcatacacaa atacgaatga
    11701 atataatatt cagacatgtg ggttccatca gacctttaaa aaataaatta tattctgatg
    11761 aaagattaaa ttgttaaaat ttttaataat cttattatca aatatatata acacttacga
    11821 tcaaaagagg gtgtcccaaa agggcacttt cttttttaaa gtaagcagcc gatttaaggg
    11881 ttaaattttg atagaaaata gctttatttt taattttatt ttggaacaag caaaaaataa
    11941 gaataaaggt gtttttagca ttttttgtcg atttgttgat tttcagttcc agttagaaca
    12001 ttgtgaacag gtttgagttg tccgtttttc atacgatatc aacataacta acattcaaag
    12061 ttaaatagtt catatttaac agtaaataag taaaacttgg gtaaatatcc gatgaatatc
    12121 ctcttttaac tgacaggaat gaaaacggtt gatcgcatga atgctacatg aaactgatcc
    12181 ggtctgcaag tgcttgctct atttttcgaa tcgaataatt gttcaaataa ctgtacaaaa
    12241 tacttcagca tcattagcgt tgattggcac ttgttcatcc tcccttttaa gtatcaatga
    12301 tttgagcaat atccaaaata tctactattt gattaaccaa ctgtgcagga gaatcttgtg
    12361 gtactttttc atccaaactg gcagggaaca aaacgatttg tccttgttca ttggatttaa
    12421 aaactactct ggacgtaatt catgttattg attaacaaat aaaaagatac aaaaaaaaaa
    12481 tatttctaca aatttattct tcctattttt aatccaatat ccattaaaaa agattgcaac
    12541 aattcttgaa cagctccatc gggaaatgga aaaaacaacc acaaattttt aggtgtatta
    12601 caaaaattct ttttcgattt gttttttgag taggtttttt acttcttctt cctctacggt
    12661 ttggaacaac tctttttgga gggaagttac gcccttgtcc acaaagccgc agggatggat
    12721 gtaattgaaa taattcaggt cggtattgac attgagcgca aagccgtgca tggtaacaaa
    12781 atgcgagctc cgcaagccaa tggcgcaaat ttttcttgcc cggggcgttt cggcatccag
    12841 ccacacccct gttgctccgg gcagtcgtgt accctttaac ccataatgtt cgagtagatg
    12901 gatgatgact tcttcgagtt tgtaaataaa aactttcagt cccagttgca atttttcaag
    12961 gtcaaaaata aaataaccaa ccacctgccc cggcccatga aaagtgatat cgccacccct
    13021 gttggtgcgg aaaaaacttg cgccgcgggt ttgcaactga atctggttga gcagtagatt
    13081 ttcttcatgt ccgtttttcc ccaacgtata aacgggaaaa tgctggcaaa aaacaacata
    13141 gtgcgtgtta aaatcggcat tttttttgtt ttgaagcagc agctgaaaca aatcttcctg
    13201 ccttttccac gcatcggcat attctgtcaa tccccaatcc aaaaattcga tttgccgggt
    13261 catggttact tttgtttttt atgttttaaa aatttaaatt ccggacgttc aaaatgcacc
    13321 ggttcgattt tgttcatcat ggccatgatg cgaccttgcc gtctcaatac atatcctgaa
    13381 ggatgggcag ggttgaaacg tcggggattg ggcagacagg cagcaatgag tgccgcttcg
    13441 gattttgtga gtttggaagc atgtttgccg aaatattcca tcgaagcggc ctccacgccg
    13501 taaattccat cgcccatttc gatcacattc aggtaaactt ccataattct ttccttgctc
    13561 cagaaaagtt cgatcaatac ggtaaaataa gcctccaatc cttttctgat gtaagagcgc
    13621 tgcggccaga ggaaaacatt ttttgccgtt tgctgcgaaa tggtactgcc tccacgaatg
    13681 cgacgacttc gtttattgtg ttcaatggct tgattgatgg cttcaaagga aaaaccatga
    13741 tgatgcaaaa actggtcgtc ttcggcagcc actgctgcct gaatcatatt gtgcgaaatt
    13801 ttgtcgatgg gcacccattt tttgctgatt ttcggcgttt cgccggagaa aacggattca
    13861 gcggtacgaa tgagcatcag cggcgtaacc attaccggga caaaccgata gataatcacc
    13921 catccaatgc taatgagaaa gaataaaacg ataaagtttt taatgaattt aaaagctttc
    13981 ctgaacataa cgaaaaaatt tgttagtcga tcagacgaaa acgtacgcgc cacctaccct
    14041 cttcataatc gtggcttata atttcaaaat gtccgatttc ggaagtattg gcaacatgct
    14101 ggccaatgca ggcgcaggca tcataatcgc caactctgac aatccgcaac acgtcggaag
    14161 cgtcatccgg gagtttcgac aaatctacta caccggaagc ttcttcccgt ttcagaaatt
    14221 cgatggttac cggcaaatga ctgtcgataa cggcatttac ccggtcggaa agttcccgaa
    14281 tttgttcctc tgtaggcgcc tgcggcaaaa gataatcaca cttgctcttt ttccgttcga
    14341 tgtgggcatt ttgagagcgc ccacaaccaa acatgcgaat catagtctgg ttgagaatat
    14401 gttcgcatgt gtgcatgggt tcgaagtatt gctttttagg cccattgatc tgattttcca
    14461 ttttggtctc ttttgcattt gtttgtcgtc tgcaaagata atgaatctca atgagaagca
    14521 actataatcg atatcttact ataaaaaaat ttctgttcgg aattaaagat ttcaaaaata
    14581 gtctgatagt catcctgtca ttcaacacag caaatagttt gtaaacatac ctataaatcg
    14641 attattggat atttttcaat ttgcaaaatt gaaataactc tcatctatac tcaacaacaa
    14701 actataaatt atctgaaatt ctttttgaga cttttcaact ttaagatttc acctccgaag
    14761 gtttaaaaaa gcgaagtcga agtgcattca gcaccaccgt aacagaactg aaactcatgg
    14821 ctgctgcggc aaatatggga ctgagtttcc agcccaacag cggataaaaa atacctgcag
    14881 ctaaaggtat accaataata ttatagaaaa atgcccaaaa gagattttcc ttaatgttcc
    14941 gtatcacggc attactcagt cgaatagccg aaacagcatc cataaggtca ttgcgcatca
    15001 acacaacatc tgccgattcg atagcaatat cggtaccggc accaatggca attcccagat
    15061 ccgcacgcat caaagccggt gcatcattga tgccatctcc caccattgcc acctttttcc
    15121 cttccttttg cagatttact attactttat ctttatcctg aggcaacact tctgaaaaaa
    15181 cggttgaaat tcccaattta cgctgaacag cagccgccgt ttttttgtga tcacctgtca
    15241 gcatgacaac ctccagtccc aaagaacgga attggttcac ggcttccttg ctggttggtt
    15301 tcaatggatc agcagcagca atcactccta ttactttttc cctggaagcc acaaacaagg
    15361 gtgtttttcc ttcttcagcc agttgagtgg caattttatc gaaattctgt acatctattt
    15421 tctgctcttc cattaattgt ctgtttccta tcagaaaata attttctccg gcttcagcta
    15481 caattccttt tccgggcaga gaagtaaaat ttttcaccga aaaagtttcc agctttcttt
    15541 tgtctgtttc ttccagaatt gcttccgcca atggatgttc agaaagtttc tccagcgaag
    15601 cggctatttg aagcaattca ttttccgtaa tgtttggtga aggaatgatg tcggtaagtt
    15661 gaggttttcc cattgtcaac gttccggttt tatccagcac taccgtatca attttgtgtg
    15721 ccatctccag cgaagcagcc gattttatca aaattccgtg ttcagctcct tttcccgttc
    15781 ccaccataat ggcaaccggc gttgcaagtc ccagagcaca cggacaggaa ataaccagaa
    15841 cggaaatggc aactgacaag gaaaaatcga aagaataccc taaagaaaac cagacaatgc
    15901 cagccgtcaa agcaataaaa ataaccacag gtacaaaaat acggcttatt ctgtcggcca
    15961 acttagaaat gggagctttt gaggaagaag cttcttccac gaggctgata atatgcgaaa
    16021 gagtagtatc attgcctact tgcgttgctt tgagcgtgat atatccggat ttgttgatgc
    16081 tggcagacaa tactcggtcg ccgctttgtt tcagtaccgg gatactttct ccggttagtg
    16141 ccgattcatc cactgtagaa cttccgcttt ccacaacgcc atccacagga attcgctgac
    16201 cggggcgaac aacaattatg tcccctactc taacttcttc agcaggaact tctacttctg
    16261 tcccgtctcg gataatagtt gccgtttttg gagacaaatc catcaattgc gtgatagccc
    16321 gagatgtttg actttttgat cgcgattcga gatattttcc aagcgtaatc aaagtcaaaa
    16381 tggttgctcc cgactcaaaa tacatatcgt gagcaaactg atgcaccaac tccggttgtg
    16441 catgaccaag cccgtaagct attttgtaca atccgtaaat tccatataaa atggccgccg
    16501 aagaacctat ggcaatcagt gaatccatat ttggagcagc cttaaaaagc attttgaaac
    16561 cgtttctgaa atacttttcg ttcgaaattg caatgggaag agtcaacaaa aactgcgtaa
    16621 aagcaaaaac cattgcattt tcttctcccc tgaaaaaagc cggtatatac caacccagca
    16681 tggaacccat agacaaatac agtaacggta accagaaacc gaaagtaatc caccaacgtt
    16741 tctgcatttc ctgttgttct ttttgaacaa aatctatttt tttcgtttcc cgactcgcag
    16801 cctgctctgc agatgatttt acatgtgctt cgtatccggc ttctacaacg gcttcttcaa
    16861 ttttatcagt atctacgttc gattcctcaa agtctactac catactgttg gtcagcagat
    16921 tgacattaac ttttttaaca ccttccagtt ttccgacctc tttttcaatg tgggctgcac
    16981 aggcagcgca actcattccc accacatcaa atttttctgt tttcatttcg gtttgttctg
    17041 aattaaaatt tctttatata aacagataaa aaactatttg gttcaagttc accaacaaag
    17101 aaaaatttcc tctagtttgt taaaatataa aaaaaaagat gaaaactaat aaaagttaat
    17161 tccattgctt tgcaaatcaa aacactgttt cctttacgtc taactgataa acaaacatta
    17221 tatttacttc taaaatttca aacattatga aaaagctcgt attattggtg ttttcgctga
    17281 tactgttttc cggtatcggt tttgcgcaga acaaatctga tgaaagttcc catccgtcat
    17341 tgccggctgc tttggaaaat ctgacccagc gggagaaaga ttccatcttg ctcaataaac
    17401 tttcgcccga acaactgttg gagctaaaaa aagcagaatt ggaaaacgaa aggaaaaaaa
    17461 ttgaagcaaa tgctcgagaa gacatgcctt ttaccgggtt tcaattattt ttaatttgtg
    17521 ttttgccttt cctttttgtc attattctga tatggattat ttatgctgca aaaaacaagg
    17581 agtcgaaacg aaaatttgaa ctgtacatga aggcgttgga aatgggacag ccgatcccgg
    17641 aagatttttt cccgaaagat aaaaaggata agccgaactc caacttaaaa aaaggcgtat
    17701 tatgggctgc agtgggactt gccttgctta tttactttat catcactcac aataatgatg
    17761 ggctgattgt gggaattgtt ccaacttttg tgggcattgg atatttnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnaaca accaattgag cagaatgagc aacagggatg
    17941 atctgcattg gattacgcaa tttaccttgc ttggcgacaa caaagctttt gaaaaactcg
    18001 ttggcaaata tcagtcgccc attcgccggt tctttttgaa tctgacaatg ggcgatgcct
    18061 cattgagcga cgatttggca caagaaacat ttatcaaggc atttgtgaat attcactcgt
    18121 ttcaagcttt gtccaacttt tccacatggc tttatcgcat cgcttacaat gtgttttacg
    18181 actcaatgcg cactcaaaaa caattttccg aactcgacgt tagggaaatt gaccttcaat
    18241 atcagtccga caatgcgttt tctgctgaaa agttggactt ttacaacgct ttgcaacact
    18301 taaaaaaaga agaacaaaca gtgctcattt tgcattatat ggaagacaaa actcagaaag
    18361 aaattgcaaa aatcatgaat tgtccggaag gaacggtaaa aacctacctg ctaaagggaa
    18421 aaaacaaatt gatggattat ctaaataagg aaggttatga caaaaaatga acaaacagaa
    18481 gaaaaattaa atgaattttt caaaacccat aaaacggaaa tacccgacaa tggtttttcc
    18541 ttaaaagtca tgcgccggtt gccaacagag aaagatcatt cctggattgt atggctaatt
    18601 ggtgcaacag gcatttcgtt tttgctgtac cattatatcc ataccgggat attgttgaat
    18661 tttatagaaa atctgctggt tatgcatttc gatatgttgc tgatttcaat agccgttttt
    18721 ccgattgtat gtggaattgt catgctgttg tggggaaaaa aagaaaaaac ttttttcggt
    18781 tgaaaaatga cgataagaaa caaaaaaaga gaaatctgca atgaagcaaa cttctcctgt
    18841 ttttaatttc tttgccgaaa agccaatgtc aaactcttct ttccttaatt ttggctttct
    18901 tgccggtcag atttctcaaa taatacagct tggctcgacg cactttacca tgcttgttga
    18961 ccgtaatact ttcgataaac ggagaatcca taggaaaaat tcgttctaca cctacatttc
    19021 ccgacatttt acgaacagta aaatgcttct ttcctccatg acccgaaatt tttataacat
    19081 cacccttaaa ttcctggatt ctttccttgt ttccttcctt aattcgataa gctactgtga
    19141 tggtatcacc acttctaaac tcaggataag tttttacttc ctgctcaaaa gattgttctg
    19201 caatttttaa taagtccatc tcacttgatt ttttaagtaa atacgcaata tatacagact
    19261 tcttttattc tgtcagagat tacgattgat tggagtgcaa aagtacgctt ttttttgaga
    19321 tttaccaaaa actcatcata ttttttcaaa attgcagagt ttaccagaaa atctttcaga
    19381 acatttataa atattcggaa attatttact tttgcatttg agacaggatt ttgttcaaaa
    19441 tatttagact tacgcttaaa aattacaatt gaaagctaca aatgaaattt aaaacgattc
    19501 ttatggcaat agccgtttta ggtgctggat gcactgaaaa agaaaatcat tccgtaatcg
    19561 ggaaacagat tcccaatatc gaaaatggac gcatgacgcc cgaagtttta tggtcttttg
    19621 gaagagttgg cggagtacaa atttctcctg atcgaaccaa aatactttat agtgttacct
    19681 attacagtat tcccgaaaat aaaggaaata ctgaactttt cattatgaac gttgatggaa
    19741 caaacaagca tcaaatcact catacagcca caaaagaagc cgaggcccga tggatgaaag
    19801 atggcgaaca cattgctttc ctctcttccg aaagtggaag caaccagtta tggacaatga
    19861 aaatcgatgg aagtgagcgc aaacaaattt ccaaccgcga gggaggcatc aatggatttc
    19921 ttttttctcc cgacgaatcg aaagtgcttt ttattgccga tgttaaatac ggtaaaactg
    19981 ctgccgacaa atatcccgat ttgccaaaag caacaggccg aattgttacc gatttaatgt
    20041 acaagcattg ggacgaatgg gtagagagca ttcctcatcc ttttatcgcc gactttaaag
    20101 acaatacgct gaaaaatgag caagatatca tgaaaggaga accttatgaa tgtcccatga
    20161 aaccttttgg tggtattgaa caactcgact ggagtcctga cgggaaaatt attgcttaca
    20221 cctgccgaaa aaaaaccggg aaagaatatg ccctttccac caattcggat atttatttct
    20281 actatctcga aaatggtaaa accgaaaata aaacagaagg aatgcagggt tacgacatca
    20341 atccaaagtt ttctcccgac gggaaatgga tggcttggga aagcatggaa cgcgatggat
    20401 acgaatcgga taaaaaccgt ttattcgtaa tgaatgttca aagcggagaa aaattggatc
    20461 tttcggaaga ttttgatcag aattgcaatg caatttcttg gagtgccgac agccgttcta
    20521 tttattttgt cagttgttgg cacgccgtta cacaaatcta caaaaccgat ttgcgaacaa
    20581 aatcaattgc tcccatcaca catggagtac acgactatga atttgtagca gaggcaagaa
    20641 atggctttat cggagtgaaa cattccatga gcaagcctga cgagatttac tttattgatc
    20701 cggttacggg aaaggaagcg gaattgtcct ttgagaacaa ggaactattg tcaaaactta
    20761 aaatgggaaa agttgaagaa cgatggatta ccactaccga caaaaagcaa atgcaaactt
    20821 gggtcattta tccgccggat tttgatcctg caaaaaaata tcctgcttta cttttttgcg
    20881 gaggcgggcc tcagtccact gtgagccaat tttggtcgta ccgctggaat ttccagctta
    20941 tggctgccaa tggctacatt attgttgcgc caaatcggag aggtgttccc ggattcggta
    21001 aagcttggct cgagcaaatc agtggcgatt acggcggtca gaatatgaaa gattatcttt
    21061 cagccatcga cgccttggca aaagagcctt ttgtaaacaa aaacaaactc ggatgtgtgg
    21121 gtgccagcta tggaggattt tccgtttatt ggctggcagg gcatcacaac aaacgattca
    21181 aggcctttat cgctcatgat ggtatgttca atctcgaaca acaatatctt gaaacggatg
    21241 aactctggtt tgtaaattgg gacttgggtg gaccctactg ggacagaaaa aatcccatcg
    21301 ttcgtcgaac ttatgccaat tcgccacatt tatttgtcga caaatgggat actccaatac
    21361 tcattattca cggagaaaaa gattatcgca tcctcgcttc acaagctatg gcagctttta
    21421 atgcagcagt tttgcagggt gttcctgcgg agatgcttat tttccctgat gaaaatcatt
    21481 gggttttaca accacaaaac ggaattttgt ggcaacgcac tttctttgag tggctggata
    21541 aatggttaaa atgaacaaaa atttttcatt ttttgatgaa aaatccgttg gacgcgatat
    21601 gtaacagcta agaaaaaaat aaaaaataaa atttgccatc tctacattgc aatttcaatt
    21661 tattttggat acttttgtat tgctcaaaag caaaagagct ataggtattg tttacagcaa
    21721 attagtgaaa aataattata aaaaatggat ctactgaaca aaatattgga gcgtgcaaaa
    21781 gcaaatatta aacgaattgt attgcccgaa ggagatgaaa cccgtacctt gcaggcagct
    21841 gaaataatat taaaagaaaa ggctgctcat ttaattttac tgggaaaaga agatgtcatt
    21901 ctaaaaacag catcgaattt gcatttaaat catattgctg aggcagaaat tatcgatccc
    21961 gaaacaagtc ctcgaaaagc agaatacatc gatttgctgt acaatattcg caaaaacaaa
    22021 ggcataacct atcaggaagc agaagaattg acgaaaaatc cgctttattt tgcctgtctg
    22081 atgattaaaa acggagatgc tgacggggaa cttgccggag ctcggaattt tacgggggat
    22141 gtgcttcgcc ctgcatttca aataattaaa actttacccg gcattcaggt tgtttccggt
    22201 gccattctca tgtttaccaa aacgcctcaa tacggcgaaa acggattgct tgtttttgcc
    22261 gactgtgcag taaatcccaa tcctacagct gaggaattgg ctcaaattgc ggtctgtaca
    22321 gctaaaacga ctcgccaaat tggagaaata gaaccacgca ttgccatgct gagtttttcc
    22381 accaaaggca gtgccagcca tgagttggta gataaggtag tgaaagctac ccgtcttgcc
    22441 cgtgaaatgg aaccgacact tttaattgac ggagaacttc aggcggatgc agctctcgtc
    22501 ccttctgttg cccatcgaaa agcgcctgaa agtgcagtag caggaaaagc aaatgtattg
    22561 attttccctg atcttcagtc gggaaacatt ggatacaaat tggtggaacg ctttggcgga
    22621 gcagaagctg ttggcccaat actgcaagga atggcagcac ctgtcaatga tctttctcgt
    22681 ggctgctcgg tagaggatat ctttcacatg attaccatta cttccaatct ggcgataaaa
    22741 taagtgtttt taccatacaa taaaaaaata tgtcccaacc aattattgaa cccatagaaa
    22801 aatatggtct gagcaagagg agtcgccgac gtactttgtt ttcaaaaatc ggagtggtcg
    22861 gttgcggaaa cgaaggaagt gctattgcaa ccattgcttt gctcaacgga atagaagttg
    22921 tttttctgga acctacagaa gaaaaaattg ccaatgctta cagccggatt gaaaggaaac
    22981 tgaataaaag aattcagaac tggggattga cggagaacga aaaaaaaatt gctctcagcc
    23041 gcgtacatgg aacaacgaaa tataaagatt ttgccggttg cgattttgtc attgaagcag
    23101 tacgctatga tgaccaaacg ggagaaaggc acgttgaaga aagaaaagaa gtttttcttc
    23161 aactcgaaaa agtcctgtct cctcaagcca tcattgcttc caatatttcc actgtggtta
    23221 tcacggaact tgcatcagaa ctgcaatata aggaacgttg catagggctt catttccttt
    23281 ccaatgttcc cggatcaaat attattgaaa tcgtcagagg gctttacact tccgacgaaa
    23341 cgtacgaaaa aatttgtcaa tttgcccgtc tgatcaatca tcaatatgtt gcagtcgccg
    23401 aatcggcagg gctggtgagt attcgtttgt ttttaataga gctcaacgaa gcctgtgcca
    23461 tgttaatgga aggcatttcc aacgttgaag acatagacaa agtacttacc gttggttttg
    23521 gtcatcgctt gggtgtattc cgaacagccg accaaatggg aatcgaaaaa attgtcaaac
    23581 ttatggaaaa tttgtatcag gaatatggca attctaaata caaaccttcg ccggtcttgt
    23641 tgcgtctttc tcgcgcaaaa cattttggca taaaaaaagg aaagggattt tacgaatatg
    23701 atgaatttgg taatgttata aaataaggag gataaaaaat gaaaatttta gtgttgaatt
    23761 gcggaagttc ctccgtaaaa taccgattgc tcgatttgga gaataacaaa gttcttggtt
    23821 cgggtggagt ggaaaagata ggcatgaaag gttccctttt gaaacatatc cgacaagaca
    23881 atcagataat tattctggaa ggtgaagtat tggaacatca gacagccatt gaatatatat
    23941 tgggagtgct taccagcgca aagcatggtt gtattaagtc gctggaagaa atagatgccg
    24001 tagggcatcg agtagttcac ggtgccgaaa aattcaattc aagtgtgctg attactgacg
    24061 aagtaatcaa aaaaatagaa gaatgcatcg atatagctcc cttgcacaat ccaccaaatt
    24121 tggccggtat taatgctatc ggcgaactat tgccgacggt tccacaagta gcagttttcg
    24181 acacggcctt tcatcaaaca atgcccgaat atgcctacat gtatgcaatt ccatattctc
    24241 tttataccaa atacggtatt cgccgatacg ggtttcacgg tactagccac cgctacgttt
    24301 cacatcgggc ctgtgaattc ttgaatatgg attacgtaca aacgaaaatc atcaccgtac
    24361 atctgggaaa tggagcctcc attaccgcca tagaaaacgg taaatcggtt gatacttcga
    24421 tgggatttac accattggaa ggattgatga tgggtacacg ctccggagat gtggatttgg
    24481 gaattgccac ttttctgatg gaaaaagaaa tgattgggcc ttctgcactt tctacacttt
    24541 tcaacaaaca tagcggtgta ttgggcgttt caggcatttc atccgatatg cgcgatattg
    24601 aaaccgccat tgcaaatggc aatgaaagag caaaactcgc tcttgacatg tatgagtacc
    24661 ggataaaaaa atatattgga gcttatgctg ctgccttgaa tggagtagat ttaatcgttt
    24721 ttaccggtgg tattggagaa aatcaggcca aaaccagaca aaacataata aaaggactga
    24781 cttatctggg gatagatgtt gatgaaaaag ccaatgctga agcacgtggc aaagaagcac
    24841 tcataagttc tgcagaatcg agggtgaaag ttgccgtaat tcctacagat gaagaattca
    24901 tgattgcttc cgatacgatg aaaataatca atgaaatgaa aagaggataa tttgcaaaca
    24961 gtaatttgat atccgtattc ttttgtattt caaaataaaa gacatatggt tcgctcttca
    25021 aaacttaatt ccttcgtaag aaaatactat cttacttttt aggcattaaa tctgtcctac
    25081 acaaccattc attttaaatt taatctatta tcaatggttc tttagctcaa ctttccaaat
    25141 ttgaataacg taacgttaaa gttttgttaa aaacacactg atattggtta gtttggtagt
    25201 caaattcagt cgattaaaat tgtaacatac tggatttcaa tgagttttat tttttagaac
    25261 aaatttgtag tacccaaact catcctccag acagtatgtt tgcttgttta tcaatgtgtt
    25321 cagctggaga agttaaacaa ttaaaagaaa ccgctgtgtg taagaacacg catggtaatg
    25381 tgaaagggtg gtagagtaat ccccctaact actcgatttt tatttcagca tttcggtact
    25441 atatttttca aacaaacgtt gagccgtttc aatagcattt ccactgggtt tttccaaaaa
    25501 attctcttga atttctccat tcacccattt tctctcaaaa tcgatacacc aggcagaaaa
    25561 atcttcttgt tcgaaagaag tgccgtttat caaggacttt tgcaagagct caataaattt
    25621 ttcccaacgt ggcaaataat aactccgcat aaggccattc cattggcgat tggcataatc
    25681 taccaattcg ccgccggact tatgccatgt ggtgataatt tcacgcgcat ttctttcgta
    25741 atagattttt tcctctctcg tttttcccca gcgacgtgca tctgaaagcc aactgccaag
    25801 taaaaattcc tttcttgttc ccaacaacaa gtcaatttcc attccaagtg aaataaactg
    25861 atgagaatat ttttgaaaat cagagagatt ttgttctcga taagcagtca tcattttttc
    25921 gtaaaggcaa ctgccataat ttccgagtgc ttgcctggcc aaattaacga catcaaaaca
    25981 ataggcatca ttttttcgac tttcttcatt tgcttgcaac atgatttgta aagctttcgc
    26041 caaatcggga ttgtcataag gaacaaatgg attggtagtc catgaacttt cctttaaatc
    26101 catgaccggc aaaacctgaa aaacaacgct atgattccca attacttgag cctcgtccac
    26161 caagactttt tcttttaaca acttccatgc ttcaatgaca gccgaatctt cctgtccggc
    26221 tcttctgaca gcataatttt ttatccattc gtccagattg aacgaagaaa tttgattgct
    26281 gcaaggaatt tccaatacca tttcataaat ttcgggattc acattcagcc cttccagcgt
    26341 agcacccacg ccaagacaat ttgccacttt tagtgcttct acaattcttt tggaaacttt
    26401 tttcatgggc gcaaccaaat gggtattccc gccaaaattt cccaaataac accaaataaa
    26461 tggcgctcca aaaaagtttt ctgtccgctt gtaaaattct tcttcctctg ccacataatc
    26521 caaaaaaatc agtttccctt tcggaacact ttcaaccata gctttgagcc gctcctgcgt
    26581 ccagtgttcg ctgtcgtagt aaaaagtcca cgacatctga taccaaatag cttctggatc
    26641 agcatttttc atcgatcggt aaattgtttt tgccacagaa gcaatatatt caggagccca
    26701 gctgggtggg tcaatttcgt tgaaaggatc ggccgaatag cgatgtgaaa tgccataaag
    26761 tttttcctgt tctttcagaa aacgatattg aatttcggaa aatagttttt ctgaaggatc
    26821 gagaaaatag gttgtataat cggcgtccat tccaccccaa cccggagaaa tttgatgaat
    26881 gttagcctcc ggataaattt gttttagctg ttcgggaaca tgtccggcaa aagccggcaa
    26941 aacaggtttc atagacaatt ctctgcatct ggcgagaatt tttttctgaa gccgatactg
    27001 accttcgata tacgaaaccg gcaaggggcc gccccattta tccaaattcg ccattcggtg
    27061 ccacggcaaa tgcgcaggcc cggaaaaata agcctgaata tcttcttttt tcaatccaaa
    27121 agattgccat acatttaacc agactttttc ctgtccggcc agcattaatg gccggttgac
    27181 cccattcatt gccagccagt cgataaattt ttcccatcgt tcccaattcc agaacggaaa
    27241 agtataacca aaagtacaag tattgttaaa aaatcgttct tgcgcattac atgccattct
    27301 tattgttgaa atcggcaaag gcaactctcc ttttatttgt aaaggtgata cggaaaacca
    27361 gtcgtaagag acatgcgcaa catcccgcaa ataacgatta aaagccatag caacagacag
    27421 ttcattgttt ccccgcaaca caacttgtcc attttttgat gtagcaatct caaaaacatc
    27481 tttcccgtgc tcagaagaaa tttgttcaat cacaaaatga tttgcctttt cgggcaacac
    27541 acgttgaatg agcgcttcag aatttttaac aaaagattga taagaagatg cataaatgct
    27601 ttgatggcag gctaccaaca ataaaataga caataggtga tttttcataa taactgaaaa
    27661 tttggttttc tcacaacaaa aataacaaat catcccgaca aaaatgagaa gagataaaat
    27721 ttatttgtgc aacaaaaaat gttggactaa tattttttaa aatattcttt taaaacgaaa
    27781 gaaataatcg tacttttgta aaaaactata aaactataaa aaaccttttt catcatgaga
    27841 ctcatcattc agccagatta cccgacttta tcgcagtggg cagccaatta cgtggcagca
    27901 aaaattcttc atgcccagcc aagcgctcaa aaaccttttg ttttgggttt gccaacggga
    27961 tcttcgcctc tcggcatgta ccattattta atcgaattaa acaaaaaagg cattatttcc
    28021 ttcgaaaatg ttatcacttt caacatggac gaatatgttg ggttaccccg tgaacatgaa
    28081 gaaagctatt acagtttcat gtggaaaaac tttttcagcc atattgatat caaacccgaa
    28141 aatgtaaata ttctcaatgg caatgctgaa gatttgatag cagaatgtgc agcttacgaa
    28201 gaaaaaatga aatcggtggg aggaattgag ctttttgtag gaggaatagg gccggacgga
    28261 cacatcgcat ttaacgagcc gggctcatct ctttcatcgc gtacgcgaca aaaaacattg
    28321 actaccgaca ccatacttgc caattcgcgt ttcttcaata acgatattaa tcaggttccc
    28381 aaaactgctc ttaccgttgg tgtgggaact ataatggatg ctcgggaagt tttgattctc
    28441 gttaatggac acaacaaggc tcgcgctctg cgccatgcag tagaagaacc tatcagtcag
    28501 atgtggacgg tgagcgcact gcaactgcat cccaaaagta ttatcgtatg cgattacgac
    28561 gcctgcattg agctgaaagt gggcacttac aaatattttc tggatattga aaaagactat
    28621 ttgaacccgg aaagtttcaa catttttcct tgaaaaaaac cgtagtaaca aacataccaa
    28681 tacctgtttg ccatgattga ttttaaaagg atgcaatcct cttctgatgc gttttttcag
    28741 caattctaca ggctgtattc ttctgctttt cctgaaaatg aacgcagaag cttacagaat
    28801 ctggaaaagg aactgaacaa tccaaacttt catgcttacg tattgatgaa taaaaacagg
    28861 tttgtgggaa ttttcaatta ctggaatttt gaaaaattta tttatatcga acattttgct
    28921 attgaagaaa agttgagaag ccagtatttg ggcacagagg cattgaaatc gtttcttgaa
    28981 aaagtaaatt tgcctgtaat actggaagtt gaaatgccca atactgatgc tgcaaaaaga
    29041 agaattgaat tttatcaccg acatgaattc gtcgaattac agaattacta ccttcagcca
    29101 ccttacgaaa gcggaggttt ccccattccc atgcagttga tgagcaacga ttacgaattt
    29161 gcaaaaaata attttgaaac aattaaacaa accatttatt tagaagttta tcactttaaa
    29221 ccggaagttt aaatatttgt ttctccaaca atgctaagag aattataatg ctcctcctgg
    29281 ttaagatatt tgaggtgaaa ggaaaattct tttttaacta ttattaatca gatatagaca
    29341 aaattcaggt gcaaagaaag cgcattttac ctatttttgt agtctgaata gagaaatttt
    29401 caaaatattt tcagaataaa caattctcaa gttatgaata gatatatgaa aaaaaccgtt
    29461 tccatcattt ttatgatgct tttatttgtt tccgcttcca atgcgcaaaa agcagtgatt
    29521 caatttcagg aaaaaattca cgactttggt caaattaagg aagaagacgg aaaagtgagc
    29581 tatcagttca agttcaccaa tacaggaaca ggaccgctcg taatcagccg agtacaggca
    29641 tcttgcggtt gcaccacacc gaattggaca aaagaaccta ttgaacccgg taaatccggt
    29701 tctattatgg ttacctataa tcctttggga cgtccgggaa tttttaacaa aactatcacc
    29761 gtattcagca atgcaacaga agaacaggta caacttcaaa ttaaaggaga agtcataccg
    29821 aaaactgatg aaaattccga ttatccgata tctgtgagtg gaatcagact gaaaaccaaa
    29881 atcgttcaaa tgaacaatgt caacaaggga accattcaaa gcagaagtat ttcttttaaa
    29941 aatacaactt cccaaaacgt aagaattact tttgaaaact tgccttcgta tgttacttca
    30001 gaagttattc ccgatattac acaacccaat gaagaaggga aaattacttt ttatctcaac
    30061 tcaaaaaact gtcagcaatg gggcccaatt tccgacgatt tttatctgat tgtcaatggt
    30121 caaaagaaat tttcggaaga caacaaactg accctttatg ccaatatagt ggaagatttc
    30181 agcaaacttt ccgtcagcga aaggcaaaac gctccaattc tggatattcc ttccaccacc
    30241 ttatcactgg gaacctttaa ggccggttca aaaatcagcg gtaaattctt aataaaaaat
    30301 atcggaaaag acgaattgaa aatacaccga attattaaca acaataagga gttaaccgtt
    30361 tcttccgttc aaaccataaa aggtggaagc aaaggagaac tgaaagttac cgtcaacacc
    30421 gccaacttgg catcgggcaa ctacaaaaaa accattaccc tgcaaaccaa cgatccgaac
    30481 aacagttttg ttatcctggt cgtaaattgg acaatcagct gacaaaaaat tctttttaga
    30541 aaattacgag ccaggatgta aaaaaaggtt acattctggc tattttattc caaaaattca
    30601 actatgcact ttcatcaaaa acatcccgaa aatgatcctt cattcaaaaa tttaagtgtc
    30661 aatgatggtg taaataagcc acctattgtc aatccctatc agaaggttaa acaaaaaaga
    30721 aaaatttatt ctgcatcaga atatgtgaaa ggcatattat cgggcgatat ttccttgttg
    30781 agccaagccg taacgctggt ggaaagttca ttgaccgaac atcaaaatat tgctcaggaa
    30841 ataatcgaaa aatgtctgcc atattccggg aaatcggtca gattgggaat aactggagtt
    30901 cccggagccg gcaaaagtac ctttatcgaa gcattgggca tgtatttggt tcaaaaagga
    30961 cacagactgg cagttttggc tatcgacccc agcagcgaaa gaaccaaagg aagcattttg
    31021 ggagacaaaa cgcgaatgga agaactttcc gtttcaaaaa atgcttttat tcgcccttct
    31081 ccttctgcag gaacgttggg cggtgttgcc cgcaaaacgc gcgaaagcat tattctttgc
    31141 gaagctgccg gtttcgacag ggtttttgtg gaaacagtcg gcgtgggaca atcggaaacc
    31201 gccgttcatt ctatggtcga tttctttctg ctgcttcaaa ttgccggagc cggagacgaa
    31261 ttgcaaggta tcaaacgcgg cattatggaa atggctgatg gaattgttat caacaaatgc
    31321 gacggaaata atatcgaaaa agcacaactg gcaaaaatac agcatcaaaa tgcccttcaa
    31381 ctttttcctc ccgctgaatc gggatggaaa cccgaagtgc tgacttgttc ggccattgaa
    31441 aagacgggaa tcgaagacgt gtggaatatg gtggaaagat acgtggaata taccaaagcc
    31501 aacggttttt tcgaatataa acgaaacgat caggcaaaat actggatgta tgaaaccatc
    31561 aacgaacagc taaaggctca tttttatcaa aatgaagaaa ttaggaaatt attgcctgaa
    31621 atcgaaaaca agattcttac caatgaactt acatcattcg tcgcagcaaa aaaattgctc
    31681 gaaacatatt tcggaaaaat caacaaaaaa taacagacaa atattcaata actttttccc
    31741 cacatggaaa tgaatcaaat ttatgctttg tcgtgtttgg aaggtttaaa aaaattaccc
    31801 gaccatagta ttgatcttat ctttaccgat ccaccatatt atcaataccg ggctcaaaac
    31861 ataaaaggac ttaaaaatca taaggatgtg gttaccgagt ttgaatttga tgattttaaa
    31921 tcggaagaag agtatttgca atttttggaa gaagtattaa aggaatgtta tcgtgtgagc
    31981 aaacccggcg ccagcggata tttatggtgc ggcgacgatt ttgtgtcttt tctcaatcgt
    32041 atggttgaac gggcaggctt tcaattcaga aaagtcattc actggcacaa aaccaatcct
    32101 ttccctgccg tacatccccg aaaaatgttt gccaacagca tggagctgat tgtacacttt
    32161 tcaaaaggca ctcccaaaac atggaattac aagagcacca acgaaatgca caattttatt
    32221 gaagcaccta tctgcatggg gaaagagaga accattcatc gaacacaaaa accattgaaa
    32281 gtgtgcattc cgtacattga aattagcagt aacgaaggcg atttggtact ggatccgttc
    32341 atgggttcgg gaacgacggc agtggcctgc aaaatgctga aaagaaattt tattggcttt
    32401 gaaatcaatc cactctattg cgaaatagcc aaccaaagat tgcaaaaagt tgaataacca
    32461 agaaattttt taggttttgc cttaccgaaa cagatgagga gctttgaaaa tggtttcttt
    32521 caatttgcat gcgcaatcgc aaatattatt ctccaaaact ccactctttt tcaacaaatc
    32581 aaattctatc agaaagtttt cggtaaaaat tccttcctgc aaattttctt caatttgttg
    32641 tagcgtccac cattttgcct gtttcacttt ttccgattgg aggtctattt tattttcaaa
    32701 attttcagga tttttatatt atatattggt agtcagcaac aattttgtaa tttgtgtgtt
    32761 gcaatccaga aaaccggaat tttttaattt aaactaatag aaattattat atctcttcct
    32821 ctaaattttc tacttcattt tccgtatcga aaacaaaaag ctgacgacga aaaatttcat
    32881 ccagagaaat ctgaaaggtt tctgtatgcg ccacacctgg tataacggtt aatttctcta
    32941 gtataatctg taacaaatgt cggttgtctt ttgcataaat tttaataaac atggaatatt
    33001 ttcctgttgc ataatggcat tcaacaattt ctggaatttt tttcaactcc tctaccacag
    33061 acttaaacat tttaatatcg cttaaaataa tcccaatata agcgcaggtt tgaaatccaa
    33121 ccttataggt atctatcaca aattcagaac ctttaattat tccgagattg cgaagttttt
    33181 gtatgcgctg atgaattgct gcccccgaaa cattacattc gcgtgcaact tcgagaaaag
    33241 ggatacgggc atcttgtgaa atcaattgca aaattttacg atccaatcta tcaatttgat
    33301 gttgtgccat aacttaatct ttaagctcag aaataaataa ttaaaaaatt tttctctaca
    33361 aatgtattta atttatttga attatctaaa ttttttctac aaattgatag tcaaaattaa
    33421 aaatattcat cttttctgta ttttttaatc cgaaaaagtc tgaattattt tataaagcaa
    33481 tattgagtcc actactaaaa tgtcgaattt caataccagc aattgataca tttaggttct
    33541 cctttgattt tataagtcaa aatcaactcg tgcgtaccat aagagttccg tctgattgca
    33601 ccggcattat agtcgtagct gtaggaaagc cgaaaggaat tcgtcaggtc aatgccaaaa
    33661 agggcaccag cttcgtgcca tgtccgataa aataaaccca gttgaaaagc attgttttca
    33721 ggggtcattt tgaagaagcc attaaagttc aattctgctt gcatcatgtt gccatactga
    33781 atcaaacaca ttcctccacc cacactgaaa aaatttcgag tatagggttt gtaggaggca
    33841 tataaaaaat tggtgttatt gaacagttcg ctgaaagagt aaaagagtga aaaaatattt
    33901 tgccctgcca acccgatttt ccagtatttg ttatttaact ccacacccag attagaatta
    33961 aaattggatt caaatttaaa ttgttcgtat gctgaagggt catcaccatt cgttaaattg
    34021 acctgactca ggtcgtagtt caatccttga taattaacgg tcaatcccat actcagattc
    34081 catgtaggat ttaatgttaa ctggtaagca taggaaaaag cgagatcgta ggtattggtg
    34141 taaccaattt tatcggccat tgccttcatt ccaatttgcg tatgaaaatc ggaaagaaaa
    34201 acggtgccat gtccgaaaaa tgtagtgggc gagcccggaa aatttatcca ttgatttctg
    34261 gcagccatgc ttatttctcc attatactga tcgtgaatgg aagcaggatt gatataataa
    34321 gtgttttccc aataattgtt tacgcgaata tttgactgtc caaacatgga aacaattact
    34381 gcaaacaaga aaatcactat cagaaaacgt attttcatta aattgctttt agcgaattac
    34441 tgtaacaaaa tttgaagcaa acttcaatcc tttgggtgta gcatacatta aaacgtaaaa
    34501 gtatgttccc tcagacaccg gtttcccttt gtatgttgca tcccagcctt ctctgccatc
    34561 aaacagcagt agtccgtttc tgttgaatac ctgaatatga aatcccttca gcaacagatc
    34621 gttttttcca tctccattgg gcgtaatggt attgggcaaa gttggagctc caatccaaat
    34681 tcgttttgta gcatattccc ggcaaccatt ggggttgatc acttgtaaat taacttcata
    34741 atacccatct cttgaaataa tgtaactatg agaaacgtga ttaccctgag ttttgtatcc
    34801 atcaccaaaa tcccagtaat aaatagcatt ggatgtgtct tccgaccaga attctatcgt
    34861 tggttggcga tcggttacca tttcctgatc ggtttgtatg gtataatgga ataaatcgaa
    34921 ataactggcc atgaaagtca aaattgaact gcatccggct tttgaagttc cttcaacaga
    34981 atacgtcccc ggtttgacaa tcaataaatt atctgctgta gaaccatcat tccagcgata
    35041 atagtctgcc cctgaaacat gaagcaacag agaatcacca atacagaata tgggttcttt
    35101 atcgagtgaa agcaccggag gagcttcaac agaaattgtc ttttcataat acagcgaaca
    35161 ttctgcgttt tgaactttca attttaccgt ataattaccc ggttttgaaa aattatattg
    35221 aggattttta atatcagaaa ctccgccatc gccaaaatcc catgaataga tatagtctgc
    35281 tttctctatg ggaatcaatg cattaaactg ataggatcgc tgattgcagt cgagaagctg
    35341 atagctgata ccttgttccg gaatttgaat gttaatagat gttgtgattt cgcattgcaa
    35401 tgcgtcagta acatgaacaa aaaccaaacc attttgaccg gtcgtcatga tctcattgtt
    35461 tgcgccacta atcgttccac ttgaccacga aatctggtag ggtggaactc ctccagtgac
    35521 tgttgcctgg ttgacttgga taatattttt agttaagcaa tcaaattcaa tatcgctatt
    35581 cacttgcaca tataaaggta gttgtctttg aatggaatgc tgaacagtga cattacatcc
    35641 aaaagaatca gttactaata ctgaataatt tcccggagga acattcaaaa tattttgtgt
    35701 tgtcattcca tttgaccatt catatttgta gggtggtctt cctcctgtaa ctgcaagcag
    35761 aatagaaccg ctatttacgt tttggcaatc ggtagcatcc ttaacttgag ccgatacagt
    35821 cagcggttgc ggctctatta tggtaaaact gcggataata tcgcaaggtt gggcatcatg
    35881 aatgcgaacc gtataaacgc ccggtttcaa ttggttccta tctactccgg catttggatc
    35941 atcatcccat gcaaaagaaa tgggtggcat tcctcctaca aaattgagtt tgatactgcc
    36001 atcattggct ccataacaac ttatttgttt taccaccggt tttacggaga atcggaatgg
    36061 ttcgggaatg atgacctgta atgacttttg gcatccatta ttatcggtaa ccgttatatc
    36121 gtatgttccg ggagaaagat tttcctgcac cattccctcg ccatagttgc tccactgaac
    36181 cgaatagggt tcaacaccgc ccgtaacgtt gagtgtaatg gaagcgttgt catctccaaa
    36241 acaagtaata gcggtagatg tagccgaaac aatcagttct tgcggttgcg taactgtaaa
    36301 ggcgtcagaa gttacagtac aatttgaagc atctcgcact gtcaaaaaat aatctcccgc
    36361 tttaaggttg gttaaatttt tattgttgct ggtataattt tcaggtcctg tccacgaata
    36421 gtaataatca taatcacctg aagttatttc ggctttggtt cctcctgtta cttccacgtc
    36481 aatacttccg gttgcatctc cgtagcaaag aacattcttt ttgtcggaaa gagaaactgt
    36541 taagggtagc ggttcaataa ttgtaaattc ttccgattgc ggagcacacg cattgccgtc
    36601 agtaaccgat acggcataag ttcctgcttg aagtgcagtc agatgattgg tgcccgtgaa
    36661 cgttgttcca tcttttgtcc atgagtaaac gtaatttccc gttcctccac ttacgttgaa
    36721 gctgatttca ccgtcggcat tgccatagca actcacattt ttttgagtta ttccggaaat
    36781 cttgattttt tcaggttcta taattgtata cgtagcttga tagggacaac ctccggcatc
    36841 caacacttga agttgataat cacctgccga taaatttgaa atatttgcat tatgtgaagt
    36901 gaatccgttg ggtccgttcc atgttatttg atacggatcg cctgtagcaa aaggtacacc
    36961 acctgttatt gtcagactta tcattccatc atttgctcca ttacacgtaa tgttgtgaac
    37021 atttgcttca atatgagctg ccggatacac ggtaaccgaa attttgaatt ctgcaccatc
    37081 acaaccgttg gctgaaggtt tgaccgtata ggttacaata gcgatagaag tggttatatt
    37141 ggtcaaagtt tgacttactt gaggttgagg atttgattgg gcacttgcac cagtaattga
    37201 attcgatgga gaaatagccg gcattgacca tgtataagtt gtacctgtcg gaacgatgtt
    37261 acttccggaa ttttcaggaa taatattgaa actttgtcca ctgcaaattg aaacgttaat
    37321 atctgaaatg acagggaatt gattaaccgt tactgcagcc ggattggaaa ctaattcgga
    37381 acatcctccc gatgaaaaag aaataacaca ataataataa attgttccga caacagcagt
    37441 agaaggcata taaactgaat cagttgctgt tggaattaag gttccaccgg tattcgagtt
    37501 caacgtattc gaaaaccatt gataggaagg aattcctact ccatttttcc atgaaactac
    37561 caacttgggt gaaatttcat ttttacaaac caccaacgat gaaggttgtt ctgtaatagc
    37621 gggaccttct ttcacaatta cttgcgaagt attgctgact gcattgcatc ccggcccatt
    37681 gggctgcgta atttcacaat agtaatataa agttccggta tttgttgtgg atggcatata
    37741 gatactgtca tttgcattag gaattattga ccctccactg ttactatttg tggtgttttg
    37801 gtaccattga taggcatatt tcccaattcc tccggtaact tttacgctga gttcttctgc
    37861 aattgaattt tgacaaatag tttgcgatgc taaaggttgt tgcgcaatgg acggatcggg
    37921 tataacttca acctttgccg tttcacttgt aacggggtcg caattgttgc ccgaaaaagt
    37981 aatcgttaca taaaaataga aagtgccggt ggcagtaaat gaagatgatg taaatataga
    38041 atcgtttgcg cccgagatag ctgttcctcc aacattggaa tctacattat tactatacca
    38101 ctgaaaagat ggaccagcac caccactgta ttttacttta agtggctgag gcaaactacc
    38161 tccaacacat attttttgtg atgcaagggg ttgttgagta atttggggag gttcattcac
    38221 agttactgta gcaatatcgg aaattatttc tctgcaataa cctgacgaaa attctatgat
    38281 acaataataa taagttgtcc caaccgtaga agttgatggt gtataaacag agtccacagc
    38341 tcctggaata ggcgtcccac ctgaagtgga ttttacatta ttggaatacc actggaaaga
    38401 aaaagttcct tctccttttg aaaaagaaac cgataaaagt gttgctgctc catctttaca
    38461 aagaatactg gaaacgggtt gtttcgtaaa atagggacgt ggataaacgt tgatgacata
    38521 tctgacagta tctcctgtgc aagtacctac ttttggtaca atggaataag ttacattgcc
    38581 ggaaacattt gcactggtta ttatattttg agccggaatt gtattgcttg ttccggaaga
    38641 aataaagcca gtaataccgg ttgaggcttg tgctgtccac gaaaatgttg ttcccggaac
    38701 agtggaagtc aatggaacgg attgagaact gtcgcctgaa caaatggttt ggcttaattc
    38761 ggtatttgta atttgaggaa caacatttat tttgaatgtc tttgttgcaa ctgtacttcc
    38821 acaaccattt tttactgtca atgaaacggt gtagtttccc ggagatgaat aggtaatttt
    38881 tcccggactt gcggctgtgg aacttgcagg tgttcctccc gcgaaactcc attcataact
    38941 gactgaatcg gtagcgggaa tacaggtttg aatagttgcg atagggctta tttcggcgct
    39001 accgcaaaaa tcgggaatat tgccgatcga aacaagaggt ggacgtgtta ccgtaacttg
    39061 ctggctgacc gtttgaacag aacagtttgt gtaaacgttc attgttaatg tatatattcc
    39121 gggattgtta aaagtaatat gtggattttt tgatgaggta gaagtgtgat caacatagtc
    39181 aaatgaggat gtagtgccgc aattggcagg ctggtaaccg acagtccaat tgtatgaagg
    39241 gttactgcat agttttgtcg tatcggttgt gttgttaact tttacggtaa atggagcaca
    39301 acccttattt ttatccaatg taaaagatgg agtaactacc ggttctacac aaatggtttg
    39361 cgtagtatcg cttgctccgc aacgattggc aattttaatt ttgatggtat agtttcccgg
    39421 atttgcaaat cttagaatga cattatcact cccagaaatc caggagctgt attgttcatc
    39481 gttcaaccct cgtgtatttc ctaatgagcc acttaccaca gtaacacctt cggttggaga
    39541 tatagaccat acaaatttat ctcctgaagt acatcctgaa cttgatgcca catatcctct
    39601 tcttgaggtg ttttgtatga aaaaatcggt atttacacaa gtagaattgt tggcattgaa
    39661 atttgcttcc ggaggaatag aaacgtagat tggagtaaca gcagatgatg ttcctgaagg
    39721 gctgcaagga tttttggcag tcatactcac cataaacgaa ttgggataat aatttctccc
    39781 atcataactg taagtaccac aagaaccatt agtaaaagta tgggttattt gggttcgggt
    39841 attatcataa aaaacctctt caggtgtacc atcgttaaat gaaaccatat aaatggtacc
    39901 gggaggatta ttttgaatac cggcaattgg aaaagtcaat tgttcattta cacaaatatc
    39961 cgtattaccc ggactaagaa tgccacctgc aggattggag cctacaaata ttttgtattc
    40021 tttggttacg gagcaaccat tttgcccttg aactgtatat tttaatgtat tcattcccaa
    40081 attataagta tgagtcagta atgaccatgt ggattccgaa aaaattggag aaccatcacc
    40141 ccaatcaata gtataattta ctatataggg attattttca ttgggattgg taaatacaaa
    40201 ttcggtggcg accttgctac aagacatgaa agtaacttga ccattcaacg tatcactttc
    40261 tgcggatgtt tccaattttg gatcgggatg ttgagataag gtgatggttt tttgttttga
    40321 attggtacag gcagtcaaat tatcagtgac ggtcagtttt accgtaaaag tttgagtgcc
    40381 gtttcctatt gccgtcataa atatatgatg tgctacactg tcggtagaag tggtattgtc
    40441 tccaaattcc cacataaaag aataatcagc gccggcaggt aagttgatag gagtaaatgt
    40501 gatgtttttt cctgcacaat tttcgtcgga aaaagtaaaa tcgacagaag gaatattgcc
    40561 tactgtaaca gtaacttgct gatttgaaat attctgagta attggcgtag catctgaaat
    40621 acttaccaga acatagttaa aaattccggc agtatttgtc ggaacatgaa tggaagcagt
    40681 gtttttccca ctttctgacg ttatggtttg taaagctcca ttatttattt tataagtgaa
    40741 gacataagga gccgttccat ttgttgctgt gaaagtaatt atcggagatg ttccattgat
    40801 gcaaacgccc aatttgtctg cattgatggt tgcagaaagt gtatttccat tattgacagg
    40861 gtaaaatgaa aatatatttt tttgatatag aaaattttcg ttcggatctg taaaatgaaa
    40921 ggcagcaaat gaataagaag aaaaaaatat catttctgtc aatgccagaa aaacacgaag
    40981 atattttttc attaattata aaaaattgat tttcagagta ttaaatttat ttggttgtca
    41041 tatatttttt attgattaaa accttgtaaa gataattaag ttattttaaa aaatagtttt
    41101 attgagaata atttgcataa attaatgata ttcatctttc ctgtattttt cggcgtggca
    41161 atcttccaaa acacttaaat agctttgata gcgcgaccag ctgatttccc ccttttcaac
    41221 agcttctctt acagcgcatt ccggttcctg tatatgcaga cagttatcgt acttgcattg
    41281 tgaagaaatt ttaaaaatct ccttaaaata atgatctact tccgattctt ccatgtcgat
    41341 caagccaaat cccttgatac ccggtgtgtc aataatataa cctccatcag gtaatcgata
    41401 catttccgaa taagtggtag tatgcatccc cttgttatga taaaaagaaa tatcggctgt
    41461 tttggcacga gcttcaggcg atagggcatt gagcaatgaa gattttccaa ctcccgaatt
    41521 gcctgccaaa agtactgttt taccggtaaa aaattctttt attgccgaaa gattttcacc
    41581 ttttaaaacc gatattttaa aaacgggata cccaatgttt tcataaagat aagcaagtgc
    41641 ttccatatac tcaacatctt ccttaccata gagatcagtt ttgttgatta tcagacaagc
    41701 aggaacatta tacgcttcgg ttacagccaa aaaacggtca ataaaaacgg tagaagtttc
    41761 gggataattt aacgtaatga taagagccgc tatatcgaga tttgcggcca tgatgctgaa
    41821 ttctttggat aaattggacg agcgacgaat aatataattt tttctgtcgt atattctgtt
    41881 aatcaggcct attccatctt cgtttttttc aaattccaca cggtcgccca cagcaatagg
    41941 attggtactt cgaatccctt ttaaacgaaa attcccttta attttacatt ctacccgttc
    42001 tttttcttca gtttcaacca gataccaact gccggtgctt tttacgacgg tgcccaacat
    42061 tacatgttca cttcaaattt attttaacaa cctttttttg agatttggaa ttacggcatt
    42121 gaaatttcat aaaaagattg ataaaacttg atccgaggtt atatcgaaag caagttttat
    42181 caattcttac cattgaaaat acccttgtta aacggtcata atctcctttt ccttgagagc
    42241 aagcatttca tcaatgattt taatgtaatc atcatgaagt ttttgaactt gttcttctgc
    42301 atctttttca acgtcttctg gaagcccttc cttaatcatt tttttgatat ggtcaatggc
    42361 atttctgcga gcatttctta tccgaatacg ggcttcttcg acttccattc taacctgttt
    42421 aaccagctgt ttgcgtcttt cttcagtcag tggtggcatg gaaagccgaa tgacttcacc
    42481 attatcggtg ggattaattc ccaaatcaga atttaaaata gctttttcaa tgggtttgag
    42541 cattgccttt tcccaaggtt gaataaggat tgtttttgca tcgggagttg taatggaagc
    42601 aacattggca agaggaacat gaacgccata ataatccaca cgaactgcat ccagaattcg
    42661 aggattagcc tttcctgcac gaatatgtgc caatgcattt tctaaaaact gtatggttgt
    42721 ttccatactt tcttttgctt catccaaaat tggttttaca tcttgtatgt ccataaaatt
    42781 tagtttttaa gattcttctt ccgtgattat ttattttcga taaggatttt caaaccaatt
    42841 caattcaatt caatttaaat tgatttttct ttatggtatc aattttttat taaagtgcca
    42901 attttttctc ccaaaataac ttttttaaga tttccaattg tatccatgtt gaatacataa
    42961 ataggcatat tgttttcctt gcatagggtt gtggcggtta aatccattat tttcagattt
    43021 ctccggtaaa cttcatcata agttatttct tcaaatctct ctgcatcgct gtttttttcg
    43081 ggatcggcag tataaacgcc atccactcgg gttcctttca gcatgatatc ggcaccgatt
    43141 tctatggcac gaagtgatga agcggtatcg gtggtaaaaa acggatttcc cgttcccccg
    43201 gaaataatga cgatttcacc tttttctaaa gatgaaacgg ctttttgttt gctgtaatat
    43261 tctcctaccg gttccatgcg tatggcggtg agcacgtgtg aagtcgctcc ttctgcctgc
    43321 aaggccgaat gaagagccaa actgttgata acggtggcaa gcattcccat ttggtcgcct
    43381 aaaatgcggt caaagccttt ttttgttcca ctcaaacccc gaaaaatatt tcctccacca
    43441 atcactatcc caacttgaac tcccattttt gcaacttctg caatttgagc cgcatattcc
    43501 tgcagccggt tttcgtcaag tccgtattgt tttcctccca ttaaagattc tccactcagt
    43561 tttaacaata tccgcctgta gtacaacatt ttgatatgtt tttaaggttc aagaaatttt
    43621 tgttttttcg ttgttatgca gaacaaacta cagataacca cacaaaaata aataaataaa
    43681 tagtatttac aaacttatga caaacacaaa aacagcaaaa tttaaaatta ttttttcctt
    43741 ttggggtttt tgggaaacca acctttttcc accctccgtt tctgctgaaa aatttcgcgt
    43801 atgctccaga aacaagaaaa tgcaaaaact cccaacaaaa ttgagggtat ttctttttcc
    43861 acaaacaaag aaacaacggc ggcaattgaa ccaattataa aaaatatcca ccagcttcgg
    43921 acaccccaaa aatactcggt ttttataaca attgggtgaa agattcctat cagaagaaaa
    43981 actgcaaagc caaccaacaa accattgaaa tttatcataa gatgagttgt ttttttgata
    44041 caaagatcga aaataatttt ataaaattaa aaaactattt gtcgagaatt cggcaaatag
    44101 ttttcgcatt cagtaatttt gaaaattttg ttggatcaac ttcaacaaat ctgatatgtg
    44161 tttaaaacac ttacatagaa ttaaaaaggg catcccgatt t
//
LOCUS       DAOTAX010000010        43094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_730_length_43094_cov_32.065917, whole genome shotgun sequence.
ACCESSION   DAOTAX010000010 DAOTAX010000000
VERSION     DAOTAX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 43094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43094
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_43094_cov_32.065917"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..2647
                     /locus_tag="PLM04_02190"
     CDS             338..2647
                     /locus_tag="PLM04_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HON01924.1"
                     /translation="MNRYLQHDPWCIIEEGFHPEKQLASESIFSLSNGHMGQRANFEE
                     YYSGETMLGSYVAGIYYPEKAERGSWKNGYSEFNDKIVNAPNWTVISVRLNDERLDIG
                     EWDLANFKRVLNMREGYLERTFEATSFKGHRIQVTVKRFLSMAENEIGVISYTVKSLN
                     YDGRISFLPLIDGDVRNYYTNYNEPFWNVLQTRTEQDVSHLWAQTRKLDFHVCCALTY
                     DFYKNRELLNVNPIKIEKEKVAGFSVGTDIKKGETVTLRKYVSVISSLNHPREELTER
                     ACSLARQAKNKGWDELFEEHKSVWAEKWRQSDVIIDNDVAAQQAIRFNIFQLNQTYSG
                     NDPRLNISPKGFTGEKFSGATRWDTEAVCVPFYLSTSPQNVARNLLLYRFRHLPKAIQ
                     NAEKLGFTNGAALFPAATFNGSEILNEWELSFEEVYRNGIIAYAIYNYVRYTNDLDYL
                     VNYGLKVLIAISRFWSQRVNISGQRKKYVILGVTGPNMYENNVNNNWFTNFIAVWCLN
                     YTMECIETIRKTNFRAFEDVAQSIGFSNEETFLWKDIVENMYFPEDKDLDIFLQQDGF
                     MDKTLTRANEIPESQRPINQHWTWDRILRSSLIKQADVVLGLYLFENFFDKETISKNF
                     DFYEPLTVHESSLSPCIHSIVACKIGKIEKGYELYLRTARLDLDDYNNEVAEGLHITS
                     MAGTWLSIVQGFGGMRIKEGLLHFYPQIPKQWQSFAFNVIFRGVHLNVRIEKSKVIIQ
                     HIKGEAIRLMIYDKSFMLLPESLIETPFE"
     gene            2688..4532
                     /locus_tag="PLM04_02195"
     CDS             2688..4532
                     /locus_tag="PLM04_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HON01925.1"
                     /translation="MKKILFLLSFLLACFASKSADKKWVDHLEPTFWWTGMNQTQLQL
                     MFHGKDVAQCDFSIQDKEVTIIRKELTDNPNYVFLYIDLQKVVSPKKFQIVLKKDKKI
                     QQYQYELKERKSNSSLRQSFTSADAVYLLMPDRFCNGNTANDSVKGYFEGVHRAKPGA
                     RHGGDIEGIISKIPYLADLGITALWTTPLFEDNDTQYSYHHYACTDYYKIDPRLGNNT
                     DYLRLADTCHAYGLKLLMDVVPNHCGWKHWWMKDIPDKNWFNTWNNYTSSNYRITAWT
                     DPHAAEADRERLIKGWFAPNMPDLNLDFPLLFDYLRQAYVFWIEYAGIDGMRVDTYPY
                     NNIKAASQFIQSILEEYPNMNVVGECWVKTPAETAYYQSGNRNADGFDSHLSSVMDFC
                     LKDVFSLAFNEKEGWENGIVRFYSHFAQDFVYPNPNFIMIFLDNHDIDRFQTAVSNDI
                     AKYKMALAMLLTLRGYPQIYYGTEIMMPGIPGSYEGHRFDFPGGWATDQRNAFTENGR
                     TTEENEIFNYLRYLLHYRKNNTVLQTGKMKQFIPENGIYVYFRYNEEKTVMIVANNNE
                     KPAELDLQRFNEMLKQYGSAKEIFSGNFYNLKNAFSIPAKSVMIYELQ"
     gene            4643..7015
                     /locus_tag="PLM04_02200"
     CDS             4643..7015
                     /locus_tag="PLM04_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HON01926.1"
                     /translation="MKNKILFSFLCTSLFFSCQPSLHYAPDGENNFNLNSLLTLEIGE
                     DTIYLQDFILNPTEIDSITSSSSWLQWKLSNDKKLLYVKVVPEMENFVDLKIWLNGTP
                     YSIPCRKTDKTDFVFTYRPQGKTYRKVQIAGQMNDWVPNFTPDLQLNDSGYYQVTLHL
                     SPGTYLYQMVLDGEWNYDVNNPLKIDNGRGKFNSILKVEGQQSLLPELYTDAYSDSKI
                     TLLSVNKVNNIFVYWQNYRLPDMFVKKQENRIIFDIPSEAHSLERSFIRVWVSNDFGV
                     SNDVFIPLHKGKVLTDSKEITRSDKASQIIYFLMVDRFKNGNKDNDHPLRRADVHPKV
                     DFWGGDLAGIRQKIDDRYFTDLGVNTLWISPVNQNPTTPYGFYAPVPTKFSGYHGYWP
                     ISSSKVDFRFGTNNELKNLVVHAHSNNINILLDYVAHHVHEEHPLYKEHPEFFTSLYL
                     PDGTLNTERWDDQRLTTWFDTFMPTLNTTDPKVIDLMTDSALFWLKEFQLDGFRHDAC
                     KHISNDFWRELTLKIKRQNHGKNLYQIGETYGSPQLIGSFLGTGMLDGQFDFNVFDEA
                     SVAFAGIGGNSLSRVNDVLQNSFKVYGHHNLMGYISGNHDRPRFMAYASGDLKYGEDT
                     KLAGWKREIGISDSTAYDKMFLLHTFNLTIPGIPVIYYGDEIGMTGANDPDCRRMMRF
                     DHWNKREQNLWKKVSTLTHLRLQHPVLIYGDFVSLQANNDSWVYARKYFDKVAIIFIN
                     NAPKSQHFEINLPSIMTNTIYQAVFQHAFTEVNGTINIDLPAYSAEILLN"
     gene            7030..7545
                     /locus_tag="PLM04_02205"
     CDS             7030..7545
                     /locus_tag="PLM04_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HON01927.1"
                     /translation="MIQIPSSELQQALEKDNKEFLRTIANTYLTAIGGNLNTSNMQLL
                     NGWQHVLLSWYFFSTEMDEGGFVQLIQNGYGGYIFDNPFAKALRLMGARELSKLIYRA
                     KKIYDENKTTLERDTSEEEFNAMYVDFEQFDELEEEYFDMEEEQLEIISAYVKGHLSD
                     FTEILANQVNF"
     gene            7719..8741
                     /gene="recA"
                     /locus_tag="PLM04_02210"
     CDS             7719..8741
                     /gene="recA"
                     /locus_tag="PLM04_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HON01928.1"
                     /translation="MAEKTTEEQKKPSSEKIKALQLAMEKIEKDYGKGTIMRMGDNQI
                     VDIDVIPTGSIGLNIALGVGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGIAAI
                     IDAEHAFDRAYAEKLGVNTDELLISQPDNGEQALEIADQLIRSSAVDIVVIDSVAALT
                     PKAELEGDMGDSKMGLQARLMSQALRKLTANISKTNTTCIFINQLREKIGVMFGNPET
                     TTGGNALKFYASVRLDIRKIGQLKDGEEVIGHQTRVRVVKNKVAPPFRKAEFDIMFGE
                     GISKSGEILDLGVEYGIIKKSGSWFSYGDTKLAQGRDAAKTVIKDNPELAAELEAKIV
                     EAIKEK"
     gene            8747..9289
                     /locus_tag="PLM04_02215"
     CDS             8747..9289
                     /locus_tag="PLM04_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON01929.1"
                     /translation="MLENENIRLRAVEPEDLELLYRWENNPELWMVGNTRNPYSRYML
                     KRYILDSEKDIYENRQLRLMITIKSSNETIGTVDLFDFDIHNSRIALGLFIEQKFQRN
                     GFAREALHLVEEYVFEFLKINQLYVQIAEENIPSMRMFEEENFEKNGMLKNWIKTTND
                     FQNIIVFQQFKNVYNKTKMK"
     gene            complement(9392..10378)
                     /locus_tag="PLM04_02220"
     CDS             complement(9392..10378)
                     /locus_tag="PLM04_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HON01930.1"
                     /translation="MKEDNSLYPLKFQPILKEKIWGGTRLKKLFNKQTESDKIGESWE
                     LSGFEGDESIVSNGFLAGNNLTELIEIYMGDLVGDKIFEEFGLTFPLLFKLIDANDDL
                     SIQVHPDDETAAQRHNSLGKTEMWYVIDAEPGGELIVGFINDCSREEYLKALEEGRVA
                     DLLKRVPVSKGDVIYIPAGLVHAIKKGVLVAEIQETSDLTYRIFDYKRTDEHEKEREL
                     HTELALDVINFSATKNPKVEYESKVNAVTTLVSCDYFTTNLLIFNRNIIRNYSSLDSF
                     VVYMCLEGNFILECENHKILVEKGETVLIPASLDEVSLIPQGEVKLLEVYIQ"
     gene            complement(10401..11441)
                     /gene="galE"
                     /locus_tag="PLM04_02225"
     CDS             complement(10401..11441)
                     /gene="galE"
                     /locus_tag="PLM04_02225"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HON01931.1"
                     /translation="MMSKVLVTGGTGYIGSHTVVELQQEGHEVIIVDNLFNSDIEVLN
                     GIEKITGIRPIFENIDCTDYVGMDRMFERHKNIDAIIHFAAYKAVGESVEKPLLYYRN
                     NILSLINLLQLMPVHKIKHLVFSSSCTVYGQPDILPVTENSPFKPALSPYGNTKQICE
                     EIIRDTIHSNSFYHSIVLRYFNPIGAHPSAEIGELPNGVPNNLLPFITQTAIGIRKQL
                     LVYGNDYNTPDGTCIRDYIDVVDLAKAHVSALNRMLNHQQKNNLEVFNLGTGRGLSVL
                     EIIKMFEKVNGVKVPYKIVGRREGDIEKVWADPSYANKELGWKAQRPLEETLRSAWEW
                     EKKLAERTKQNA"
     gene            complement(11448..12353)
                     /locus_tag="PLM04_02230"
     CDS             complement(11448..12353)
                     /locus_tag="PLM04_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HON01932.1"
                     /translation="MKPTLLILAAGMGSRYGGLKQLDGLGPNGETIMDYSIYDAIQAG
                     FGKVVFVIRKSFEKEFNELIVSKFKNLIDVELVFQELDKVPEGCTYATEREKPWGTNH
                     ALLMAKDVIHEPFGVINADDFYGRESYFILADFLRSVENKKNEYCMIGYRVGNTLSES
                     GPVSRGICVADENNFLKDVVERTHIVEKGGTIIYLDENGKEVAIHPNVPVSMNMWGFT
                     PDYFDYSWEAFKKFLAENGQNVKAEFYIPTVVNQLIHENKVTCKILDTPSKWFGVTYS
                     NDRPQTVLKINELIRKGVYPEKLFK"
     gene            12697..13515
                     /gene="ispE"
                     /locus_tag="PLM04_02235"
     CDS             12697..13515
                     /gene="ispE"
                     /locus_tag="PLM04_02235"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HON01933.1"
                     /translation="MIVHPNAKINIGLNITGLLPNGYHSIETVFYPVNVYDHLKIEPI
                     PNAAENQFVVKGLSIDGKPENNLVIKAWQLLRDEYKIAPVKIFLEKNIPMGAGLGGGS
                     SDAAFMLKTLNDLFQLDIDREKLRNLAAKLGADCPFFIDNQPVFAQGKGDEFHPLKLS
                     LKNFYLVLVKPDIHIATANAYAETVPKKPEFPLQELVKKPIEEWKYFIKNDFEESIFK
                     RYPLIAEIKQKLYETGAVYASMSGSGSAVYGIFYDRPVRNGYFDNYFFSIVPLS"
     gene            13575..14012
                     /locus_tag="PLM04_02240"
     CDS             13575..14012
                     /locus_tag="PLM04_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HON01934.1"
                     /translation="METTNKIQKIIGNQETFTNIGLLIFRLIIGIFMLTHGLQKITNF
                     EMLKAFFPDPLGIGASTSLILIILAEVGCSLLIIFGIFTRLAAIPPMFGMAVAAFMVH
                     ANDPFSVKEIAFLYLFLYVALLFLGAGKYSIDYLLTKHLTKKK"
     gene            14293..16830
                     /gene="gyrA"
                     /locus_tag="PLM04_02245"
     CDS             14293..16830
                     /gene="gyrA"
                     /locus_tag="PLM04_02245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HON01935.1"
                     /translation="MIEQERIIKVNIDEEMKSSYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLFGMNELGNTPDKPYKKCARIVGEVMGKYHPHGDSSIYGTLVRLAQPWSMRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLQKIAEEMLADIDKDTVDFQPNFDETLKEPVVLPS
                     RLPNLIVNGSSGIAVGMATNMAPHNLSETVDAIVAYIDNNDISIQELTKYIKAPDFPT
                     GGIIYGYAGVKEAFETGRGRIVIRSKVEIETEASGREKIVVKEIPYQVNKVELIKTIA
                     NLVEEKKIEGISHINDESDREGMRIVIEVKRDANSNVVLNKLYKLTALQSTFNVNNIA
                     LVHGRPHMLNIKDLIRYFVEHRHDVVIRRTKFELAEAEKRAHLLEGFMIILDNLDEAI
                     QIIRNSKTPDEARSNLMSRFNLTEIQARAIVEMRLRQLTGMEQEKLRAEYQEILALIA
                     HLKEILDKIELRMQIIKDELLEIKAKYGDNRRTEIVYSSEEFNPEDFYSNDEMIITIS
                     HLGYIKRTPLSEFRTQGRGGIGSKGSDSRDEDFIEHIYPASMHNTMLFFTQKGKCYWL
                     KVYEIPEGSKSSKGRAIQNLLNIDGDDKVTAFIRVTGLTDPDYNSSHYLVFCTKKGTI
                     KKTSLEAYSHPRQNGVTAINIREDDEVIQVRLTDGNSEILMANRNGRAIRFHESTVPE
                     MGRVSTGVRGMKLDDEKDEVVGMICVNKEDNTNILVVSEQGYGKRTLLDDENGEPIYR
                     ITNRGGKGVKTMNITEKTGKLVAIKNVTDDNDLMIINKSGIAIRLKVADIPVIGRATQ
                     GVRLINLEKRNDEIASVCKVDSEPVEEIVDEETIETELAENNEPNEN"
     gene            17000..18229
                     /locus_tag="PLM04_02250"
     CDS             17000..18229
                     /locus_tag="PLM04_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON01936.1"
                     /translation="MKKIILSFFLVLSFTFTYAQKSNVSKAKNKALMENPDFAGAREA
                     IQAALNDSITKNDPNTWYVAGLIGYKENDYWYQKLILNQKYDADAKGQAILESYNYFK
                     KAAELDQLPDAKGKVKPKFLKDIKTKIKEYYTLQPNLIGYGAYLYEKKDYEGALKVFE
                     TYLEIPTLPYLKDEIVIDSTYYMIQYFAALSATNAGYHDKAISLYEDLKHKNYETLNV
                     YELLADEYRTINDTIKYVETLKEGLEKFPSELWFLQNLINHYIYSGKIQDALAYLNDA
                     IKRDPNIAQYYFVKGNLEENLGNYDEAISAFNKAIELDPNLAEAYAGIGRLYYNKAIK
                     MLEVANDIKDVKLFNQEKEKANKVFKESIPYFQKAKELNPNEIEYKRTLRTLYYRLQM
                     DKEFEAIDEEIRAMENQ"
     gene            complement(18616..18984)
                     /locus_tag="PLM04_02255"
     CDS             complement(18616..18984)
                     /locus_tag="PLM04_02255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HON01937.1"
                     /translation="MNTAIVILGSNVDADQNIDAAKERLSEYFEIDLCSSRIGSDAVG
                     ENYVAPFLNEAVKLYSDETAEETIAIFKQIETEMGRTPESKITGLVPIDIDLIFWNDN
                     LMRNDYNRFSFVKRCVDEIK"
     gene            complement(19107..19613)
                     /locus_tag="PLM04_02260"
     CDS             complement(19107..19613)
                     /locus_tag="PLM04_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HON01938.1"
                     /translation="MKPNQFENELIALQNNMRNFAFSLTLNREDAEDLLQDTTLKVLD
                     NREKFTKNVNFKGWVLTIMKNIFINNYRKIVRNQTVIDKTEDMYHLNLPQNSGFDSPE
                     GSYSIGEINSNIEAFPDEYRIPFSMHLQGYKYEEIAETMHLPIGTVKSRIFLARKRLQ
                     QQLKDYRD"
     gene            19896..21251
                     /locus_tag="PLM04_02265"
     CDS             19896..21251
                     /locus_tag="PLM04_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HON01939.1"
                     /translation="MCFITENLNASNKKTTVFVIELKKDIGGTAWVYCQKAFNQAKEK
                     NADVILMHMNTYGGEVVFADSIRTRILNSPIPVYVFIDNNAASAGALIAIACDKIFMR
                     PGAAIGAATVVNTTGQQMPDKYQSYMRATMRTTAEAHGKDTIIQGNDTIFQWKRDPKI
                     AEAMVDDKVSIPGIIEAGKTLTFTAEEALQHGYCDGIAESIDEIIKNHITEANFEIIV
                     FQPSFWDKVKGFFSSSIVRSILIMLIIGGIYFELQTPGIGFPLAISILAAVLFFVPLY
                     IDGLAANWEILIFLLGLVLLALEIFVIPGFGIAGISGILFSVAGLTLSLINNVNFNFD
                     GVQAGTVGKSLLTVLVGITLGFVLILYLSNKIGSTGIFKKMALNTALENKEGYIGVSM
                     DELQLVGKTGYASTVLRPSGKVNVEGKVYDAVSTEGFIEKGVQVRVLEYRTGQIYVEK
                     I"
     assembly_gap    21273..21372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21565..22821
                     /locus_tag="PLM04_02270"
     CDS             21565..22821
                     /locus_tag="PLM04_02270"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HON01940.1"
                     /translation="MIVYKFGGASVRSAAGIKNLASIVSGVNDNLFIVVSAMGKTTNA
                     LEGVLEDFMQADHASALAKFQQIENYHNEILAELFPDTENPVAILQPLYDEITSMLKL
                     SVGDDYDRWYDKIVSYGEIISTQIVSKFLNEAGIQNGWLDMRKLLITDSNFREANVKM
                     KESQQKLTEAVNFENYPVYIGQGFIGANTKGDPTTLGREGSDYTAAVVANLLNAENLT
                     IWKDVPGILNADPRLYPQAKLVPELTYSEAVELAYSGAQVIHPKTIKPLQNKNIPLYV
                     RPFGNPSEKGSVIKAHVEKPAGIPFYSLLKNQVLVTIRPIDFSFVLEENLPKVFSMIN
                     KHRLKVSMIESSAISISVAIGNSRYLPAALDELSNEFDVSYNENLELLTVRGISDEIA
                     QEVTNHREVLLMQRTRRIGRFLMKER"
     gene            complement(22903..24270)
                     /locus_tag="PLM04_02275"
     CDS             complement(22903..24270)
                     /locus_tag="PLM04_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HON01941.1"
                     /translation="MEKTKKFILTEKELPTHWYNIVAEMQNKPMPILNPATKQPMKEE
                     ELYPLFAEELSHQELNQTDAWIEIPEEVREKYKIYRPTPLVRAYDLEKALDTPAHIYF
                     KNESVSPVGSHKLNTALPQAYYCKQEGVTHLTTETGAGQWGTALAFAAKTFGLELFVY
                     MVKISYEQKPYRRSLMQTWGAQVIASPSMSTNAGRKILSEHPNYLGSLGTAISEAIEL
                     AMNTPNCKYTLGSVLNHVSLHQTVIGLEAEKQMEMAGEYPDIVIGCFGGGSNFSGISF
                     PFLRHNIKDGKKTRFIAAEPASCPKLTRGVFEYDFGDEVGYTPLLPMFTLGHNFAPPH
                     IHAGGLRYHGAGTIVSQLKKDNLVEAVDIDQLDSFKAGVLFAQTEGIVPAPESAHAIA
                     VAIHEALKAKEEGTPKTILFNLSGHGMVDMTAYDQYFAGDLTNYSLSENDIAENIKNL
                     EHLAK"
     gene            complement(24379..24465)
                     /locus_tag="PLM04_02280"
     tRNA            complement(24379..24465)
                     /locus_tag="PLM04_02280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24429..24431),aa:Ser,seq:gct)
     gene            24669..25700
                     /gene="mltG"
                     /locus_tag="PLM04_02285"
     CDS             24669..25700
                     /gene="mltG"
                     /locus_tag="PLM04_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HON01942.1"
                     /translation="MKKKVRKILFAALAVPVIVVIYLTYVFFAPNIFPKKETETFVCI
                     PENTSFEDVVKLIKNESRVWDSYSMRLTAKIFDYDKNIRSGRYALKKGMNNFALIRNL
                     RSGRQVPLRLTFNNIRTKEQLAGRLSSQLMTDSLTFLKLLNNSQFLSQYALNPDNSVV
                     IFIPDTYEVFWNIKPKKLFDRMYREYLKFWNEERRQKAASIPLTPIEVSILASIVEEE
                     SNKSYEQPIIAGVYINRLKNKMPLQADPTIKFAWKDFSLKRIKGQHLQINSPFNTYKF
                     QGLPPGPIRLPSPQVIDAVLNYTHHSYLYMCAKETLNGEHNFATTHAEHLINARKYQR
                     ALNERQIFE"
     gene            complement(26069..>26503)
                     /locus_tag="PLM04_02290"
     CDS             complement(26069..>26503)
                     /locus_tag="PLM04_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HON01943.1"
                     /translation="QIVTLQQCFEDEQTQIATLAKIVHQTEEYDYILNPNRPKIVLNK
                     NNEALYFSRQVIPYQRNTANEHWISNHQYFVHVGIYAYRTEILKELCSLPVSSLEKAE
                     SLEQLRWLENGYRIKVGFTTAESYGIDTPEDLEKVRKMFLGK"
     assembly_gap    26504..26603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26604..26710)
                     /locus_tag="PLM04_02295"
     CDS             complement(<26604..26710)
                     /locus_tag="PLM04_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HON01944.1"
                     /translation="MTLHSNRNFRFVGIIPARFASTRFPGKPLADIGGKP"
     gene            complement(26744..27991)
                     /locus_tag="PLM04_02300"
     CDS             complement(26744..27991)
                     /locus_tag="PLM04_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HON01945.1"
                     /translation="MKRAIFVSLLSFILFPILSQNFTFSDSAKVSLLTCSPGKKVYEK
                     FGHTAIRVNDPITGYDVVFNFGVFDFNTKNFYLKFIKGDTDYQLAVYDTKSFLEEYKS
                     RNSMVWEQKLNLTLNEKRNLLHSLLINYEPENKFYRYNFVYDNCSTRPRDKIVQSVNG
                     KIEYHLYNEPVTFREMIAQYTGVNSWMQFGLDLIFGRDADKSVSRNVSMFLPENLMYE
                     FQAAHIIAADGAKRHLVAENKILIPKQEEKYSKPLPIFQPVFVCTFLLILGIIITIFN
                     NKKSFRLFDSLLYIVSGLGGIIVFYLAFFSSHPLVKNNFNLLWLNPLNFALGILLWFH
                     KMKPYVFYYQVLNCLLIFLALVAYAAGLQVFHPASFPLILLLLLRTVRWTATTKKDFS
                     LKKKNNRDYYTNSNGLHHHSRKN"
     gene            28268..28969
                     /locus_tag="PLM04_02305"
     CDS             28268..28969
                     /locus_tag="PLM04_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HON01946.1"
                     /translation="MTKKPAYELKPIEEVFMVWDVLTPDERQFVRNNYTVHHFKKNEL
                     IQSEGEISTHMMILAAGKAKVYKDGIGSRTQIIRMLKPGENFGYRAIIANEPNSTNVA
                     AFEASTVYMLKADLFLSILRHNIAVCYRFLEELASDLAVSDARTVNLTQKHIRGRLAE
                     ALLFLKKRYGVEEDGATVNIYLSREDLANLSNMTTSNAIRTLSNFTNEHIIAVDGRKI
                     KIIDEDKLRKISKMG"
     gene            29045..31258
                     /locus_tag="PLM04_02310"
     CDS             29045..31258
                     /locus_tag="PLM04_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON01947.1"
                     /translation="MNATQIDTTYKAILRFVSASRLKPAFDRIKMMIDDLQYGEFSDR
                     FNELERNYKFLLDYYLKGVQDPERKSVYSKLVAKLLILLADVREELMMKNSSNFEYSQ
                     KRFFPYTRKYQTVNELFNALQYFHKQSALLSENKEQYKIELSRMRSNFEDALDELFGV
                     FWLATRYTEEENNLYKKLLEADDKYWVEKSLMVSAVTLNLWRKFDEQKIMLLFDSCEN
                     ADQKVVQRALVGLTFILAKYNIYLPYFPSIRNRLVLLVDQNRIAENFQNIIIQIIGTT
                     ETEKISRKLREEILPEMMKINPLLRDKMDAENFLNSDEWAEANPEWQEIFDESGLSDK
                     LKELSDLQLEGADVYMSTFSMLKNFTFFSQFSHWFLPFDTENSAVNQLFESDEKTILS
                     AFVNSNVMCNSDKYSFCLSVLQMPESQRNMLKHSFKMESDQMDEMEEEMSILSPETIT
                     KNISKQYIQDLFRFFKLQPHKEDFEDMFSYSLTLHRTWLFDILSSIPGFSESVAEYYF
                     SKGYYLQALELYEKIQSESQPSAALYQKMGYAYQQTSQLQKALDAYLKADMIQPDDGW
                     TIRKIALCYRLLGNYEKALAFYQHADFLKPGQYSTQIHIAQCYFELGKFKDALAIYYK
                     LDAENVDDIKVWKAIAKCNFFDGNLDKADYYSEKIILAEPNAYDYLLSGHISWCRRQL
                     SAAANAYRKALEMLQNKWEVFQDLFNEDKDRLISFGIREEELPLMMDALLYYENE"
     gene            complement(31277..32449)
                     /locus_tag="PLM04_02315"
     CDS             complement(31277..32449)
                     /locus_tag="PLM04_02315"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HON01948.1"
                     /translation="MFFAIYSFGFAQTFKNYSLSADFIYGEILKHNKHLENLVKGPLK
                     GGEFSIEWQTMGEKAWHQYLNFPSIGISTAFLDFSHPDTLGYAVALYPYLKFPIIRQQ
                     HFNMAFKAGGGLSYVTKTFYNATAYHPDGSVYLNKSNAAIGSHVNVYLTANLNMEIPL
                     TKGWSITSNWGWNHTSNGSIVQPNSGINMLDMYLGIKYFPNYERYIPPKPQPLPDLPH
                     KIEIEMALTGGIRQLYYKDDKTYPIGTFNIAAYRTMTNFYRMGWGADLFYDGVFNGKS
                     AFKRTYLTTDELKYKLRIGASFRHELLVGRLTTGFHFGLYLYNPVKNLEPYEEAKNGT
                     LNKPLIYKYNIEKEDGWFYTKAVLKYALDEHFLLSLGLKTHLQKAEFIDAGIGYKF"
     gene            32610..33341
                     /gene="gldF"
                     /locus_tag="PLM04_02320"
     CDS             32610..33341
                     /gene="gldF"
                     /locus_tag="PLM04_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HON01949.1"
                     /translation="MISIFKKELGSFFSSATGYLVIGIFLALTGLFLWVIPGEFNILE
                     SGYANVDGLFYLAPWLFLFLCPAITMRSFAEEKKSGTWEILITKPLSITELVLGKFLA
                     GWILVLIALFPTVLYYFSVYFLAEPVGNIDAGAFWGSFIGLIFLSGVYIAIGIFSSSL
                     SNNQIISFVVSVLLCFIFFYGFDLLGSFFSSGAVIDFVQNLGINAHYKSMSRGVLDMR
                     DVGYFLFLGGLFLGIMVWKNSKSDK"
     gene            33416..34174
                     /gene="surE"
                     /locus_tag="PLM04_02325"
     CDS             33416..34174
                     /gene="surE"
                     /locus_tag="PLM04_02325"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HON01950.1"
                     /translation="MNKNLILISNDDGIDAKGIKILTKLMMQLGDVVVVAPDIPRSAQ
                     SSALTVTQPIRYRKIEEEEGMVRYSCTGTPTDCVKIALNEILNRKPDLLVSGINHGSN
                     SAINVIYSGTMGAVFEGCENGIPSIGFSLASHFPDPDFSFFEPHVLKIAQIVMEKGLP
                     ESVCLNVNAPCGEIHGVRVARQCKGYWTKEFAARTDPAGRAYYWLTGYFENMEPDAED
                     TDEWAISHGFISIVPDKIDLTAYDAMDLIREWNL"
     gene            34162..34470
                     /locus_tag="PLM04_02330"
     CDS             34162..34470
                     /locus_tag="PLM04_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01951.1"
                     /translation="MEFVNSYAVKRLNHFYIGFLVGLILPCVFVWLYITSFYPVDISF
                     FEILKRLYPGVLLGKLLLLSIVPDLLMAFVFYKNDAFRLTSGTIVGGLPFLIASLFML
                     "
     gene            34520..35674
                     /gene="lpxB"
                     /locus_tag="PLM04_02335"
     CDS             34520..35674
                     /gene="lpxB"
                     /locus_tag="PLM04_02335"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HON01952.1"
                     /translation="MKYFLIAGEASGDLHASHLMHELKKQDLQAEFCFLGGDLMWTEG
                     GEMIRHYRQMAFMGFIAVLKNLSVISVNIKDGKQALLKFQPDVLILVDYPSFNLRMAE
                     FAKKKLNIPVYYYISPKIWAWKEFRIKKIRRYVDRMFTIFPFETEFYAKHRYPVEYVG
                     NPTVDAVEEFKKAYRGNFQTFRQSNNLVDKPVIALLAGSRRQEIKSCLPRMVEAAMQF
                     PDYQPVVAGAPGVEKDFYFSVLKDTSVAVVFGQTYELLFHAEVAIVNSGTATLETALI
                     GTPQVVVYHVAFGKVAYWLKKLFIKVKYISLVNLIAGKEVVMELYAHLFTVENVKNEL
                     NRILTDAHYRQNMKNEYKKVSEILGEPGAASRAAKKIFELLHSDKSVLTP"
     gene            complement(35664..36572)
                     /locus_tag="PLM04_02340"
     CDS             complement(35664..36572)
                     /locus_tag="PLM04_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HON01953.1"
                     /translation="MESISSFAELIAHLKFLNNRKRVAVANAVDPHTLEAVLRASDNG
                     FVEAFLIGDLAMIESPHFFEHKLTPYVHIIDIPEVEEATREAVRMVRTGEADILMKGL
                     VNTDVILRAILNKENGLVEKGKIITYNAALEIPDYQKLLFFTDPAVIPSPTVEQRTAM
                     IKYVTETAYKFGITRPKIALIHATEKANPKIQYMQDYLDIMEKWRMGEFGDVIMDGPL
                     DIFLALDPERGSIKKVSSPIHGDADILIFPSFECANAFYKGLMVFAKAEMAGLLQGTQ
                     KPVVLTSRSESVNSKYYSLAMACVMG"
     gene            complement(36778..38049)
                     /gene="serS"
                     /locus_tag="PLM04_02345"
     CDS             complement(36778..38049)
                     /gene="serS"
                     /locus_tag="PLM04_02345"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HON01954.1"
                     /translation="MLTLKYIAENSEDVLKRLKKKHFDGKELISKVLELNAERKKTQY
                     DADSIAAEINKISKEIGILYKQGKTSDAETLKQKTLELKENIKLLEEKQNQIETQLNE
                     ILVQIPNLPHESVPEGKLAEDNQVERTGGKIPSLPTDALPHWDLAKKYDIIDFETGVK
                     ITGAGFPLYKGKCARLQRALINFFLDNAREAGYMEVQPPYVVNEASAYATGQLPDKEG
                     QMYFCQEDNLYLIPTAEVPVTNIYRGMILNENELPIKNVAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPEKSYETFYEMVHYVEGLVEKLELPWRTLRLCGGDMSFTSA
                     LTFDIEVYSAAQQRWLEVSSVSNFESYQANRLKCRYRSADKKIELCHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALIPYTGFEIID"
     gene            complement(38225..38485)
                     /gene="rpmA"
                     /locus_tag="PLM04_02350"
     CDS             complement(38225..38485)
                     /gene="rpmA"
                     /locus_tag="PLM04_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HON01955.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIYGGQFAKAGNIIVRQRGTV
                     HHPGKNIGMGKDHTLYALVDGKVEFRKKVGNRSYVSINPEMN"
     gene            complement(38505..38819)
                     /gene="rplU"
                     /locus_tag="PLM04_02355"
     CDS             complement(38505..38819)
                     /gene="rplU"
                     /locus_tag="PLM04_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HON01956.1"
                     /translation="MYAIVEILGQQFKVQAGKKLYVHRMEEAEIGSQVEFDKVLLVDN
                     DGSIAVGAPCVEGAKVVCDVVGHVRGEKVIVFHKKRRKGYRKRTGHRQDFTELLIKEI
                     VA"
     gene            39136..40962
                     /locus_tag="PLM04_02360"
     CDS             39136..40962
                     /locus_tag="PLM04_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HON01957.1"
                     /translation="MEYQLTDFLPVTRKELELRAWEDVDVVFFTGDAYVDHPAFANAL
                     IGRLMESLGLRVAIVPQPNWRDDLRDFKKMGRPRMFFAVSAGNMDSMVNHYTANKRLR
                     SNDAYSPDGKPGFRPDYATVVYCNILKKLYPDVPILIGGIEASLRRLTHYDYWSDQLK
                     QSILCDAQADLLIYGMGELPLTELIRQLKNGKQFHELTDIPQTVYLSDNIPQDNLWNT
                     IELPSFETCLHDRKKYASAFKTIEEQSNRYQADRLIQKTEDKWVVVNPPYPPMIDAQL
                     DASYELPYTRMPHPKYKGKTIPAYEMIKFSVNIHRGCFGGCSFCTISAHQGKFIVSRS
                     KKSILNEVEKIKLMPDFKGYLSDLGGPSANMYQMKGKNIEICKKCKKPSCIFPKVCFN
                     LNTDHHKLLDIYRSVDALQGIKKSFIGSGIRYDLLLHESADELTKKSQSEYIRELILH
                     HVSGRLKVAPEHTSDRVLAIMRKPSFQYFIRFKTQFDRINKEANLNQQIIPYFISSHP
                     GCTKEDMAELAVITKNLDFKLEQIQDFTPTPMTLATEIYYSGFHPYSLQPVYVPKTKE
                     EKLEQRKFFFWYKPEYRSQIKRDLLMMQRPDLLKKLFQNERK"
     gene            41040..41397
                     /gene="rnpB"
                     /locus_tag="PLM04_02365"
     ncRNA           41040..41397
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM04_02365"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            41482..42810
                     /locus_tag="PLM04_02370"
     CDS             41482..42810
                     /locus_tag="PLM04_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HON01958.1"
                     /translation="MASPINFFVRIYNFFVSDIWKIAENELTRTRRFFYHLIKIILYA
                     IRGYSTDKLAIKASALTYSILFAVVPVIAIIIAIGRGFGMETLIEDALKNTFIAQANL
                     VPVVMDFVKKYLESIQGGVFLGVGIVILLSAVVNFFMQVEDTFNSIWQVKKSRSFLRQ
                     FTTYFSIILILPLFIVLSSGFSIFVNTTISQIPGFQILNPLLKVGANLLPYLINWLIF
                     TFMYLFIPNTQVRFINALIAGIIAGTAFQLFQILYITGQINLSRYNAVYGGFAAIPLL
                     ILWIRISCLIVLLGAELTYASQNIRNYESELDTQNISIRNRKLISLYITYLVIKQFEL
                     QKPPLSSEQIASDFKLPIRLVNQILNQLVNISVLIEVFNDKNKSKSYQPAMDINQLTV
                     GLLFEKLESYGTGITLLKNNEKLKNFWEKTSAVLAAGSKESSNEILIKDL"
     gene            complement(42846..42945)
                     /gene="ffs"
                     /locus_tag="PLM04_02375"
     ncRNA           complement(42846..42945)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLM04_02375"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT        13144 a         7832 c         8359 g        13559 t
ORIGIN      
        1 tctgttttta aatttttttg ataaatcttt ttcgtatgaa acccacatgt attacataca
       61 caaacacgaa taatgaatgt aatattcaga catgtgggtt ccatcagacc attaaaaaat
      121 aaattatatt ctgatgaaat attgtttttt tctctgaaaa atcgaataat tgaaatatat
      181 ttgcattact atataataag cttgacaaaa ttagttaaaa ttttcttgag aaaatttgtg
      241 aagccaaaaa tggaatagtc gcgcggtttc gatttcaaat gtcgggttta cattatcagt
      301 ggaataaaca cataagcaaa cacataattc aattgctatg aacagatatt tacaacatga
      361 tccttggtgc attatcgaag aaggttttca tccggagaaa caattggctt ccgaaagtat
      421 tttcagctta agcaatggtc acatggggca acgggcaaat tttgaagaat attattcggg
      481 tgaaaccatg ttgggttctt acgtggccgg catttattat cccgaaaaag ctgagcgggg
      541 aagttggaaa aacggctatt ccgaatttaa cgacaaaatt gtgaatgccc ccaattggac
      601 agtcatcagt gttcgtttaa atgatgaaag gctggacata ggcgaatggg accttgccaa
      661 ctttaaacgt gtgttgaaca tgcgcgaagg ttatctggaa agaacttttg aagccacatc
      721 attcaaggga caccggattc aggttactgt aaagcggttt ttgagcatgg ctgaaaatga
      781 aatcggcgtc atcagttata cggtaaagtc tttgaattat gatggccgta tttcttttct
      841 tcccttgata gatggagatg tgcgaaatta ttataccaat tacaacgaac ctttctggaa
      901 tgttcttcaa accagaacgg aacaggatgt ttctcattta tgggcacaaa cccgtaaatt
      961 ggattttcat gtttgttgtg cgctcactta cgatttttac aaaaatagag aattgctgaa
     1021 tgtcaatccc attaaaattg aaaaggaaaa agttgcaggt tttagcgtag gtacggacat
     1081 taaaaaaggg gaaaccgtta cgttgagaaa atatgtttcg gttatcagct cgctgaatca
     1141 tccgcgcgaa gaacttacgg aacgtgcctg ttccctagcc cgacaggcaa aaaataaagg
     1201 ctgggatgag ctgtttgaag aacacaaatc ggtgtgggca gaaaaatggc gtcagtccga
     1261 tgtaattatc gataacgacg tagctgcaca gcaagccata cggtttaata tttttcaact
     1321 caaccaaacc tacagtggaa atgatccccg attgaacatc agcccgaaag gtttcacggg
     1381 cgaaaaattt tcaggagcca cacgctggga taccgaagcg gtgtgcgtgc cgttttatct
     1441 ttccacttcg ccgcagaatg tggcacgaaa tcttttactt tatcgtttcc ggcatttgcc
     1501 aaaagccatt cagaatgccg aaaaacttgg tttcacaaat ggcgcagccc ttttccctgc
     1561 tgcaactttc aatggcagcg aaattctcaa cgaatgggaa ctgtcttttg aagaagttta
     1621 tcgaaacggc attatagctt atgctattta caattacgtg cgctatacca acgacttgga
     1681 ttatcttgtc aattacggat taaaggtact gattgccatc tcacgtttct ggagtcagcg
     1741 ggttaatatt tcgggacaga gaaaaaaata tgttattttg ggcgtaacag gaccaaacat
     1801 gtacgaaaat aacgtaaaca acaactggtt tacaaatttc attgccgttt ggtgtttgaa
     1861 ttatactatg gaatgtattg aaaccatacg aaaaaccaac ttccgtgctt ttgaagatgt
     1921 ggctcaaagt attggcttct ccaatgaaga aacatttctg tggaaagata tagtcgaaaa
     1981 catgtatttc cccgaagaca aggatttgga catttttctc cagcaagatg gtttcatgga
     2041 taagacactc accagagcca atgaaatacc tgaaagtcag cgccccatca atcaacattg
     2101 gacgtgggat cgaatacttc gttcctcgct tatcaaacaa gctgatgtag tacttggact
     2161 ttatcttttt gaaaattttt tcgataaaga aacgataagc aaaaatttcg atttttacga
     2221 accgctcact gtacacgaat cttccttatc gccttgcatc cattcgatag tggcctgtaa
     2281 aattggaaag attgaaaaag gttatgaact gtatctgcgc acggcacgac tcgatttgga
     2341 cgattacaac aatgaagttg ccgaaggcct gcatattaca agtatggccg gaacctggct
     2401 ttccattgtt caaggttttg gcggaatgcg cataaaagaa ggccttttgc atttctatcc
     2461 gcaaattcct aaacagtggc aatcgtttgc tttcaatgtg attttcagag gggtacattt
     2521 aaatgttcgt atcgaaaaat cgaaagtaat cattcaacat atcaaaggtg aagccatcag
     2581 gctgatgatt tacgacaaat cttttatgct tttgccggaa agtttgattg aaacgccttt
     2641 tgaataacaa atattgaagt ccattaaaaa ataatttgtt taccaacatg aaaaaaattc
     2701 ttttcctgct ttcttttctt ttggcttgtt tcgcatcaaa atcagccgat aaaaaatggg
     2761 ttgaccacct tgaaccaact ttttggtgga caggtatgaa ccaaacacaa ctccaactaa
     2821 tgtttcatgg gaaggacgtg gcacaatgcg atttttcgat tcaggacaag gaggtaacca
     2881 ttatccgtaa agaattgacc gataacccaa attatgtttt tctttatatc gacctgcaaa
     2941 aggtggtttc gccgaaaaaa tttcaaatcg tcctgaaaaa agataagaaa attcagcaat
     3001 accagtacga actgaaagag cgcaagtcca attcttcatt gcggcagagt tttacttcgg
     3061 cagatgctgt ttatctgctg atgcccgaca gattttgcaa tggcaatacg gcaaacgatt
     3121 ctgtgaaagg atattttgag ggtgtacatc gtgcaaagcc aggagcgcgt catggtggcg
     3181 acatcgaagg tattatttcc aaaattcctt atttggccga tttgggtatc acagctttgt
     3241 ggactacgcc gttgttcgaa gacaacgata cgcaatattc ctatcatcat tacgcctgca
     3301 ccgattatta caaaattgat ccgcgtctgg gaaacaatac cgattatttg cgactggccg
     3361 acacttgtca tgcttatgga ttaaaattat tgatggatgt agttcccaat cattgtggct
     3421 ggaaacattg gtggatgaaa gacattccgg ataaaaactg gttcaatact tggaacaatt
     3481 atacctcttc caattaccga attacggctt ggactgatcc tcatgctgcg gaagccgatc
     3541 gcgaaagact cataaaaggc tggtttgctc ccaatatgcc cgatttaaac cttgattttc
     3601 cgcttctttt cgattacttg agacaagctt atgttttttg gatagaatat gccggaattg
     3661 acggaatgcg tgtggatact tatccgtaca acaacataaa ggctgcttcg caatttattc
     3721 aatcgattct cgaagaatat cccaacatga atgttgtggg cgaatgttgg gtgaaaacgc
     3781 ctgcagaaac agcttattat caatcgggca acagaaatgc ggatggattt gactcccatt
     3841 tgtcgagtgt aatggatttt tgtctgaaag acgtgttttc cttagccttt aacgaaaaag
     3901 aaggatggga aaatggaata gttcgttttt acagtcattt tgcgcaggat tttgtttatc
     3961 caaatccgaa tttcatcatg atttttttgg acaatcatga tattgatcgg ttccaaacgg
     4021 ccgtcagtaa tgatattgcc aaatacaaaa tggctttggc catgttactg acactgcgcg
     4081 gttatccaca aatatattat ggcacggaaa tcatgatgcc tggcattccg ggtagttacg
     4141 aaggtcatcg gttcgatttt cccggtggct gggctaccga ccaacgtaat gcttttacag
     4201 aaaatggcag aacgacagaa gaaaacgaaa ttttcaacta tttgcgatat ttgttgcatt
     4261 accggaaaaa caatactgtg cttcaaaccg gaaaaatgaa acagtttatt cccgaaaacg
     4321 gaatttatgt ctatttccgt tacaatgaag aaaaaacggt tatgattgtt gccaacaaca
     4381 acgaaaagcc cgcagaattg gatctgcaaa gattcaacga aatgttgaag caatatggca
     4441 gcgctaaaga aattttcagc ggaaattttt acaacttgaa aaacgctttt tccattccgg
     4501 ctaaaagtgt gatgatttac gaacttcaat aaacctgtta ccaagttatg aaacccacat
     4561 gtattgcata cacaaacacg aatgaatata atattcagac atgtgggttc catcagacct
     4621 ttaaaaaata aattatattc tgatgaaaaa caaaattctt ttttcctttt tgtgtacaag
     4681 tctgttcttt tcttgccagc catctttaca ttatgctccc gatggtgaaa acaattttaa
     4741 cttaaattct cttttaacat tggaaattgg ggaagatacc atttatttac aggattttat
     4801 actgaatcct acagaaatcg actccataac ttcgtcttcc tcctggttac aatggaaatt
     4861 atccaacgac aaaaaattac tttatgttaa ggttgtgccg gaaatggaaa attttgtaga
     4921 tttaaaaatt tggttgaacg gaacgcctta ttccatcccc tgccgaaaaa ccgacaaaac
     4981 agattttgtt ttcacttatc gaccacaggg aaaaacatat cgaaaagttc aaattgcagg
     5041 acaaatgaac gactgggttc ccaactttac gcccgacttg caacttaacg acagcggcta
     5101 ttatcaggta actttgcatt taagtccggg aacttatctt tatcagatgg tgttggatgg
     5161 agaatggaat tacgatgtca acaatccgct taaaatcgac aatggaaggg gaaaattcaa
     5221 ttccatattg aaagttgaag gccagcagtc gctgcttccc gaactttaca ccgatgcgta
     5281 ttccgattcc aaaataacac ttttatcggt aaacaaggta aacaatattt ttgtttactg
     5341 gcaaaattac cgtttgcccg acatgtttgt gaagaaacag gaaaaccgca ttattttcga
     5401 tattccttcc gaagctcatt ctctggaacg ttcgtttatt cgtgtttggg tttccaatga
     5461 ttttggcgtc agcaacgatg ttttcattcc gcttcacaag ggaaaagtac tgacggactc
     5521 aaaagaaatt acgcgttcgg ataaagcatc ccaaattatt tattttctga tggtcgatcg
     5581 gtttaaaaac ggcaataagg ataacgatca tccccttcgt cgagctgatg tacatcctaa
     5641 agtcgatttt tggggtggcg atttggccgg gattcgacaa aaaattgatg accggtattt
     5701 taccgatttg ggagtaaata ctttgtggat ttctccggtg aatcaaaatc cgacaactcc
     5761 ttatggattt tatgcaccgg tacctacaaa attttcaggg tatcacggtt attggccgat
     5821 ttcttcttcc aaagtcgatt ttcggtttgg tacaaacaac gaactgaaaa atcttgtggt
     5881 ccacgctcat tccaacaata tcaatatttt actggattat gttgcccatc atgtacacga
     5941 agaacatcca ctttacaagg aacatccgga gttttttact tcattgtatc ttccggacgg
     6001 aacattgaat acggaaagat gggatgacca gcggttgact acgtggtttg atacatttat
     6061 gccgactttg aacaccaccg atccgaaagt gatcgattta atgactgatt cagcactgtt
     6121 ttggttaaaa gaatttcaac tcgacggctt tcgacacgat gcctgcaagc atatttcaaa
     6181 tgacttctgg agagaactca ctctaaaaat aaaacgtcaa aatcatggaa aaaatttata
     6241 tcaaattggc gaaacatatg gaagtccaca gcttattggc agttttttag gcaccggcat
     6301 gttggatggt caattcgatt ttaatgtttt tgatgaagct tccgttgctt ttgccggcat
     6361 tggcggaaat tctctaagtc gggtaaacga tgtgttgcaa aacagtttta aggtgtatgg
     6421 acatcacaac ttaatgggct atatttcagg caatcacgat cgtccccgat ttatggctta
     6481 tgcttcgggc gatttgaaat acggagaaga caccaaactg gccggatgga aaagggaaat
     6541 aggaatttca gattctacgg cttacgataa aatgttttta ttgcatactt ttaacttaac
     6601 tattcccggt attcccgtta tttattatgg cgacgaaatt ggcatgacgg gcgccaatga
     6661 tcccgattgc aggagaatga tgcgttttga ccattggaac aagcgagagc aaaatttatg
     6721 gaaaaaagtt tctactttga ctcatttaag acttcaacat ccggtactga tttatggcga
     6781 ttttgtttct cttcaggcta ataacgacag ctgggtatat gcccgaaaat attttgataa
     6841 ggtggccata atatttatca ataatgcgcc caaatcccag cattttgaaa tcaatcttcc
     6901 gagcatcatg acaaacacaa tttatcaggc tgttttccaa catgctttta cagaggttaa
     6961 cggaaccatc aacatagact tgccggccta cagtgccgaa atattactca attagaaaaa
     7021 tacaaaaaaa tgatacaaat cccaagttca gaattgcagc aagcccttga aaaagacaat
     7081 aaagaatttc ttcggacgat tgccaatacc tatttgacag ctattggtgg taatttgaat
     7141 acttccaaca tgcaattgct caatgggtgg caacatgttt tgctttcgtg gtattttttt
     7201 tcgaccgaaa tggatgaagg cggttttgtt caactgattc aaaacggtta cggagggtat
     7261 atttttgaca atccttttgc caaagcatta cgattgatgg gagcacgtga gctgtcgaaa
     7321 ctgatttaca gggcaaaaaa aatttatgac gagaacaaaa ccacgctgga aagagatact
     7381 tccgaagaag aatttaatgc aatgtatgtt gattttgagc aatttgatga attggaagaa
     7441 gaatatttcg acatggaaga agaacaactg gaaataattt cagcatatgt aaaaggacat
     7501 ctttccgatt ttactgaaat acttgccaat caggtcaatt tttaaaattc tatgtttaac
     7561 actaaaaata aagtactgat tttaaaaata ttaaacatct tccggtcaag atttatgttc
     7621 actcacattt gtagatccat tcattttcat gtatttttgt atcagtgaat ttatattaaa
     7681 gaagttcaga aaaataatca attattaaaa acttaattat ggccgaaaaa acaaccgaag
     7741 aacaaaagaa accgtcttcc gaaaaaataa aagccttgca gctggcaatg gaaaaaatag
     7801 aaaaagatta cggaaaaggg accattatgc gtatgggcga caaccaaata gttgacatcg
     7861 atgtgattcc aaccggctcc ataggtttaa acattgcttt aggcgttggc ggctatccca
     7921 gaggcagagt gattgaaatt tacggacccg aatcgtccgg aaaaaccaca ttggccattc
     7981 atgccattgc tgaagcccag aaagccggcg gcattgctgc cattattgat gcagaacatg
     8041 ctttcgatcg tgcttatgcc gaaaagttgg gtgtaaatac cgacgaattg ctgatttctc
     8101 agcccgacaa tggcgaacag gcgttggaaa ttgccgatca actgattcgt tcgtcagccg
     8161 ttgacattgt tgttatcgat tccgttgccg ccttaactcc aaaagccgaa ctggaaggcg
     8221 acatgggcga ttcaaaaatg ggacttcaag cccgattaat gtcgcaggca cttcgcaaac
     8281 ttactgccaa cattagcaaa accaacacaa cctgtatttt catcaatcaa cttcgggaaa
     8341 aaattggtgt tatgtttggc aatcctgaaa ctactaccgg aggaaacgcc ctgaaattct
     8401 atgcttccgt tcgtttggat attcgaaaaa tcgggcaact gaaagatgga gaagaagtta
     8461 taggacatca aacgcgtgtg agagtagtga aaaataaagt tgcacctcct ttccgtaaag
     8521 cagaatttga tattatgttt ggcgaaggaa tttccaaaag cggtgaaatc ctcgatttgg
     8581 gcgtagaata cggaattatc aaaaaaagtg gttcgtggtt cagctatggc gataccaaac
     8641 ttgctcaggg aagagatgcc gcaaaaaccg taattaagga caatccagaa ttggctgccg
     8701 aactcgaagc aaaaattgtt gaagctatca aagaaaaata aaattgttgc tggaaaacga
     8761 aaacatacgg ctacgtgctg tcgaacccga agatttggaa cttctgtatc gatgggaaaa
     8821 caatcccgaa ttgtggatgg tgggcaatac ccgaaatccc tattcacgtt atatgctgaa
     8881 acggtacata ctggattcgg aaaaagacat atacgaaaac aggcaattgc ggttgatgat
     8941 aactatcaaa agcagcaatg aaaccattgg aacggttgac ttgtttgact tcgacattca
     9001 caatagccgc atagcattgg gactttttat cgagcaaaaa tttcaaagaa acggttttgc
     9061 cagagaagca cttcatctcg ttgaagaata tgtatttgaa tttttgaaaa ttaaccaact
     9121 ttacgtacaa attgctgaag aaaatatccc aagcatgcgc atgtttgaag aagaaaattt
     9181 tgaaaaaaac ggcatgttga aaaattggat aaaaaccacg aacgattttc aaaatattat
     9241 cgttttccaa caattcaaaa atgtttacaa taaaacaaaa atgaagtgat gtgatagatt
     9301 tgttttgaca gaataaaaca ggaaaaaggg aatggcactt caactctcgt ttgcagccat
     9361 tcccttttta atattaagtt tgaaataatc cttactgaat atatacttcc agcaatttta
     9421 cctctccttg cggaatcaat gaaacttcgt ccaaactggc aggaattaaa actgtttcgc
     9481 ccttttccac caagatttta tggttttcgc attctaaaat gaaatttcct tccaaacaca
     9541 tataaactac aaaggaatcc agcgaactgt aattgcggat aatattacga ttaaatatta
     9601 gcagattggt ggtaaaataa tcacacgaaa ccagtgttgt taccgcatta actttagatt
     9661 cgtattcaac ttttggattt tttgtggcgg aaaaattaat gacatccaaa gccaattctg
     9721 tatgcagctc acgctctttt tcatgttcat cggttcgttt gtagtcaaaa atacgataag
     9781 taagatcact tgtctcctga atttctgcaa ccaatacgcc tttttttatg gcgtgtacca
     9841 aacctgccgg aatataaatc acatcaccct tgctgaccgg aactcttttc aataaatcgg
     9901 ccacacgtcc ttcttccaaa gctttcaaat attcttcgcg tgagcaatcg tttataaatc
     9961 caactatcaa ttctcctccc ggctcagcat ctattacata ccacatttcg gtttttccca
    10021 acgaattgtg ccgctgagct gccgtctcat cgtcgggatg cacctgaatg gacaaatcgt
    10081 cattggcatc aataagttta aaaagcaagg gaaaagtcaa cccaaattct tcaaaaatct
    10141 tatccccaac caagtctccc atatatattt caatcaattc cgtcaaatta ttcccggcaa
    10201 ggaaaccatt cgatacaatt gattcatcac cttcaaaacc ggaaagttcc caactttcac
    10261 caatcttgtc ggattcggtt tgcttgttga aaagtttttt tagccgcgtt cctccccaaa
    10321 ttttttcttt taaaatgggt tgaaatttta agggatataa gctattgtct tctttcattt
    10381 taattgaatg aaatttttta tcaagcattt tgtttggtac gttcagccag ttttttctcc
    10441 cattcccatg cactgcgaag cgtttcttcc aaaggtcgct gagctttcca acccaattcc
    10501 ttattggcat aactcggatc ggcccaaact ttttctatat caccttcccg tcttcccaca
    10561 attttataag gtaccttgac cccattaact ttctcaaaca ttttgataat ttccagcacc
    10621 gaaagtcctc tgcccgttcc aagattgaaa acttccagat tgtttttctg ctgatgattc
    10681 aacatgcggt tgagagccga aacatgtgct tttgccaaat caacgacatc gatgtagtcg
    10741 cgaatacatg ttccatcagg cgtattataa tcgtttccat aaaccagcaa ctgtttcctg
    10801 attccgatag ctgtttgagt aataaacggg agcaaattat tgggcacgcc attaggcaat
    10861 tcaccaattt cggccgaagg atgcgctcca atcggattga aatatcgaag aacaatgcta
    10921 tgatagaatg aattggaatg aatggtatcg cgaataattt cttcacatat ttgtttggta
    10981 ttgccataag gagaaagtgc cggcttaaaa ggtgaatttt ccgttaccgg caaaatatcc
    11041 ggctgtccat aaacggtgca cgaagacgaa aaaaccaaat gcttgatttt atgcaccggc
    11101 atcagttgca ataaattgat cagagaaaga atattattcc tgtaatacag caaaggcttt
    11161 tcaaccgact cgcctacagc tttatacgct gcaaaatgaa ttatggcgtc tatattctta
    11221 tgtctttcaa acatgcgatc catgccaaca taatccgtac agtcgatatt ttcgaatatg
    11281 ggacgaatac cggtaatttt ttcgattccg tttaaaacct caatatcgga attaaaaaga
    11341 ttatccacaa taataacttc atgtccttcc tgttgcaatt caaccacagt atgcgaacca
    11401 atatatccgg ttccgccggt aaccaaaact ttactcatga tgtctgttta tttgaataat
    11461 ttttctggat atactccttt acggattaat tcattgattt ttaaaactgt ctgcggtctg
    11521 tcattggaat atgttacacc aaaccacttg gaaggcgtat ccaaaatttt gcaggtaact
    11581 ttattttcgt gtatcaattg attgacaacc gtcgggatat aaaattcggc tttaacattt
    11641 tgaccatttt cagctaaaaa tttcttgaaa gcttcccaag aatagtcgaa ataatcggga
    11701 gtaaaacccc acatattcat tgatacgggt acattgggat gaatggcaac ttcctttccg
    11761 ttttcatcca aataaattat tgtacctccc ttttccacaa tatgcgttct ttccaccacg
    11821 tcctttaaaa aattgttttc gtcagccaca catattcctc tgctcaccgg gccactttcc
    11881 gaaagcgtgt tacctactcg ataaccaatc atgcaatatt cattcttttt attttctacg
    11941 ctgcgcagaa aatcggccaa aataaaatac gactcacgac cataaaaatc atcggcgttg
    12001 attacgccaa aaggttcgtg aataacgtcc tttgccatca gcagtgcatg atttgttccc
    12061 caaggttttt cgcgttcggt ggcataagta catccttctg gcactttgtc gagttcctga
    12121 aataccaact caacatcaat taaatttttg aatttactga caatcaattc attaaattcc
    12181 ttttcaaagc tttttcgaat cacgaacact acctttccaa atcctgcctg tatcgcatca
    12241 taaatggaat aatccataat ggtttctcca ttggggccta gcccatccaa ttgcttcaat
    12301 ccgccataac ggctgcccat tccggctgct aaaattaata aagttggttt catattttat
    12361 tttctgttgt ttatttcaaa gatttaatat agtttacaga taatcaatgt tttcagaaaa
    12421 aatttattag gaaaaattcg agtttgcgct aaaagtttta ggaaatgcaa atttactatt
    12481 attgtttcaa ttaagaaaac tgtaaactac ttcataaact taactttgac aaaagtcgaa
    12541 attttataat ttcgttaaaa tttgctattt acaacttcaa acaacaataa aaaacagtat
    12601 gagattattt gaacaaaaat tttttgtaat agaaaaaatt gaaatcaaaa aatttactac
    12661 ttttacaacc ccacaaacaa aattttaccg attcttatga ttgtccatcc caatgccaaa
    12721 atcaatatag gcctaaacat cacaggcctg ctgccaaatg gttatcactc catcgaaaca
    12781 gttttttatc cagtcaatgt ttatgaccat ctgaaaattg aacctattcc gaatgctgcc
    12841 gaaaatcaat ttgttgtaaa gggtttgtcc attgacggca aaccggaaaa caatctggtt
    12901 ataaaagcat ggcaactact gcgggatgaa tacaaaatag cgccggtaaa aatttttctg
    12961 gaaaaaaata ttcctatggg tgccggcttg ggcggtggtt cttccgatgc agcatttatg
    13021 ctgaaaacac tcaacgacct ttttcaactc gatatcgaca gagaaaaatt aagaaatttg
    13081 gctgccaaac ttggcgccga ttgtcctttt tttatcgaca atcagccggt ttttgctcag
    13141 ggaaaaggag atgaatttca tccgttgaaa ctttcgttga aaaactttta tttggtgctg
    13201 gtaaaaccag atattcacat cgctactgcc aatgcttatg ccgaaacggt gccaaaaaaa
    13261 ccggaatttc ccttgcagga actggtgaaa aaacccattg aagaatggaa atattttatc
    13321 aaaaacgatt tcgaagaatc gatttttaaa cgttatccgc tgatagccga aataaaacaa
    13381 aaactctacg aaacaggcgc cgtttatgct tccatgtcag gttctggatc ggccgtttac
    13441 ggcatttttt acgatcggcc tgtccgcaat ggctatttcg ataattattt cttttcgata
    13501 gttccattga gctaaagaaa aacattttct taacttgttt gttgttattg gtaaacaatt
    13561 taattaattt aaatatggaa accacaaaca aaatacaaaa aattattggt aatcaggaaa
    13621 ctttcaccaa tatcggctta ttgattttca ggctgataat tggcatattc atgttgacac
    13681 atggcttgca aaaaataact aattttgaaa tgctgaaagc ttttttcccc gatcctttgg
    13741 gaattggtgc ttctacttcg ctaatattaa tcattttggc tgaagtaggt tgttccctgt
    13801 taatcatttt tggcattttt actcgattag ctgcaattcc tcccatgttt ggcatggccg
    13861 tagctgcatt tatggttcat gccaacgatc cgttttccgt caaagaaatc gcgtttcttt
    13921 acctttttct ttatgttgca ttgttattcc tcggtgcagg aaaatattcg atagattatc
    13981 ttctcacaaa acatctgacg aagaaaaaat aaatcggatt cattttcatt tctttcagtc
    14041 ctattatgac ggaaacattt tcaaaatggt gttttcgtct tttttattcc tcagctcttt
    14101 tttattaacg ttagcaaact gattaacaac tattcgttaa aaaattcaaa aattttccaa
    14161 aatgaaattt aatcctcatt ttcaattaaa tatctatcaa taacataaaa aataatcgta
    14221 aaaaatcttg tccaattgag taaaaagtag taatttagag caatttttcg ataaaaataa
    14281 aaaattgcat agatgattga acaagaaaga attattaaag tgaacattga cgaagaaatg
    14341 aaatcttcgt acattgatta ttccatgtcg gtaattgtat ccagagcttt acctgatgtt
    14401 cgcgatggtt ttaagccggt acaccggcgt gtgcttttcg gaatgaacga attgggcaac
    14461 actcccgata aaccttataa aaaatgtgca agaattgtgg gtgaagtgat gggaaaatat
    14521 catccgcacg gcgattcttc gatttatgga acattggttc gactggctca accttggtca
    14581 atgcgttatc cactggtaga tggtcaggga aattttggtt cggtagatgg tgatagtcct
    14641 gcagccatgc gttatacaga agcccgtctg caaaaaattg ccgaagaaat gctggccgat
    14701 attgataaag acactgttga ttttcagcca aattttgacg aaactttaaa agaaccggtg
    14761 gttcttccct cacgcttacc caacttaatt gtcaacggtt cttcggggat tgccgtcggc
    14821 atggctacca acatggcgcc tcacaattta tcggaaaccg tagatgcgat cgttgcctat
    14881 atcgacaaca acgatatttc cattcaggaa ttgacaaaat atatcaaagc acccgatttc
    14941 cctaccggcg gaattattta tggttatgcc ggagtaaagg aagcttttga aaccggacga
    15001 ggccgcattg tcatccgctc caaagttgaa atagaaacgg aagcttcagg gcgcgaaaaa
    15061 atcgtggtta aagaaatacc ttatcaggtt aacaaggtgg aattgataaa aaccattgcc
    15121 aatctggtag aagaaaaaaa gattgaagga atttcacata tcaacgacga atccgaccgt
    15181 gaaggaatgc gcatcgttat tgaagttaaa cgcgatgcca attccaatgt ggtgcttaac
    15241 aaactttaca agctgacagc cttacaatca actttcaatg tcaacaacat tgctttggtt
    15301 catggcagac ctcacatgtt gaatatcaag gatttgattc gctactttgt tgaacatcgg
    15361 cacgatgttg ttattcgtcg aaccaaattt gaactggccg aagccgaaaa aagagcacac
    15421 ttgctggaag gatttatgat tatcctcgat aatctggacg aagccattca aataatccgc
    15481 aattctaaaa ctcctgacga agcacgctcc aatttaatga gccgcttcaa ccttaccgaa
    15541 attcaagctc gcgctattgt agaaatgaga ctgcgtcaac ttaccgggat ggaacaggaa
    15601 aaacttcgtg ccgaatatca ggaaatcctt gcattgattg ctcatttgaa ggaaattctc
    15661 gataaaatag aacttcgtat gcaaatcata aaggatgaac tgttggaaat aaaagcaaaa
    15721 tatggtgata accgtcgtac cgaaattgtt tattcttcag aagaattcaa tccggaagat
    15781 ttctattcga atgatgaaat gattattact atttctcatt tgggatacat caaaagaact
    15841 ccacttagtg aattcaggac acagggacgt ggcggcatcg gttcaaaagg cagcgattcg
    15901 agagacgaag attttatcga acacatttat cctgcttcaa tgcacaacac catgttgttt
    15961 ttcacgcaaa aaggcaaatg ctactggctg aaagtgtatg aaattcccga aggcagcaaa
    16021 agctcaaaag gtagagccat tcaaaatctt ctgaatatcg acggagacga taaagttaca
    16081 gcatttatcc gcgtaaccgg cttaacggat cccgactaca acagctcaca ttacctggtt
    16141 ttctgtacca aaaagggaac aattaaaaaa acctcactgg aagcctactc ccatcctcgc
    16201 caaaatggag taacagccat taatattcgc gaagatgatg aagtaattca agtaaggctg
    16261 accgatggaa attcggaaat tttaatggct aaccgcaacg gccgtgccat tcggttccat
    16321 gaatcaaccg tgccggaaat gggacgtgtt tctaccggcg ttcgcggcat gaaactcgac
    16381 gatgaaaaag atgaagttgt tggcatgatt tgtgttaaca aggaagacaa caccaacatt
    16441 ctggtcgtat ctgaacaagg ttacggaaaa cgaactttac tggatgacga aaatggagaa
    16501 ccaatttacc gcattaccaa cagaggtgga aaaggcgtaa aaacgatgaa tatcaccgaa
    16561 aaaacaggaa aattggttgc cattaagaat gttaccgatg acaacgattt aatgattatc
    16621 aacaaatcgg gaattgccat tcgactgaaa gttgccgata ttccggttat tggtcgtgcc
    16681 actcagggtg tgcgtttgat caacttggaa aaacgcaatg atgagattgc ttccgtttgc
    16741 aaagtagatt ccgaaccggt agaagaaatt gttgacgaag aaacaataga aaccgaatta
    16801 gcggaaaata acgaaccaaa tgaaaattaa ttaaaaaata tttggaaaac aataatttgc
    16861 attgtaattt tatgccctga aaaaaatatt tttttaaaaa ataaatattt actgcaagtt
    16921 gatttcattt gtcaatcaac ttgcagtaaa tcaattggaa gagtaaaatt ttattgtaaa
    16981 aaaatttaat aataaaagta tgaaaaaaat cattttatct ttctttttgg tgctaagttt
    17041 caccttcact tatgctcaaa agagcaatgt gtcaaaagcg aaaaacaaag cattgatgga
    17101 aaatccagat tttgcaggag ctcgtgaagc cattcaagct gctttgaacg attctataac
    17161 gaaaaatgat cccaacactt ggtatgttgc cggattaatt ggatataaag aaaacgatta
    17221 ttggtatcag aaattgattc tcaatcaaaa atacgatgct gacgctaaag gacaagccat
    17281 tttggaatcg tacaattatt tcaaaaaggc tgccgaattg gatcaactcc ccgatgcaaa
    17341 aggtaaagta aaaccaaaat ttttgaaaga tatcaaaaca aaaattaagg aatactatac
    17401 cttacagccc aatttaatcg gctatggtgc atacctttat gaaaagaaag actatgaagg
    17461 agcattaaag gtatttgaaa cttatcttga aattccaact ttgccttacc taaaggatga
    17521 aattgtgatt gattctactt actatatgat acaatatttt gcagccctgt ccgctactaa
    17581 cgcaggttat catgataaag ccatcagcct ttacgaagat ttgaaacata aaaattatga
    17641 aaccttaaat gtatatgaac tgttggcaga tgaatataga actattaatg ataccattaa
    17701 atatgttgaa acgctcaagg aagggttaga aaagtttcct tctgaattat ggtttttaca
    17761 aaatcttatc aatcattata tttatagtgg taaaatacag gatgctttgg catatcttaa
    17821 tgatgctatc aaacgcgatc cgaacattgc acaatattat tttgtaaagg ggaatctgga
    17881 agaaaatttg gggaattacg atgaagcaat ttcagctttt aacaaagcaa tcgaattaga
    17941 tcccaattta gctgaagcgt atgctggcat aggccgattg tattacaaca aagccattaa
    18001 aatgttggaa gttgccaatg atattaagga tgtgaaattg tttaaccagg aaaaagagaa
    18061 agcaaataaa gtctttaaag aatcaattcc ttattttcag aaagcaaaag aattgaatcc
    18121 caacgaaata gaatacaaac gtactttgcg taccttgtat tatcgtttgc aaatggataa
    18181 ggaatttgaa gctattgatg aagaaataag agctatggaa aatcaatgat aaaatcaatt
    18241 tttatacaat tatctatata taggagaaaa ttatttaggg ttttctcctt tttttttatt
    18301 catttttaaa ttaaaacatg agtccgcttc gaaaagtcaa aaaactaaat tcatcgactt
    18361 tttaacctta tttcttataa gttgctgaaa aacgagtttt ctttttggtc gggggcgaag
    18421 cgaattttca aaaataaaac ttttcggagt agatttaaac atgaatgaat gtttaccgat
    18481 aaaatttttt cgtgatttcc ttcattgctt ccgaatttcg ctttagcttc catagtggct
    18541 accaaagtac gttttccgcc tctgtttctg aatttgcaaa gattaattcc ctaaaccgac
    18601 aaagagaaaa tttgattact tgatttcgtc aacacatctt tttacaaaag agaagcggtt
    18661 gtagtcgttg cgcattaaat tgtcgttcca gaaaatcaaa tcaatatcaa tcgggacaag
    18721 tcctgttatt ttactctcgg gagttcggcc catttcggtt tctatttgct tgaaaatggc
    18781 tattgtctct tcggctgttt catcggaata aagtttcacg gcttcgttca aaaatggtgc
    18841 aacatagttt tcaccaacgg catcgctgcc aatgcgggaa ctgcataagt ctatttcaaa
    18901 atattccgac aacctttctt ttgcagcgtc gatattttga tcagcgtcaa cattgcttcc
    18961 aagtataacg atggcggtat tcataaaact tttttaaata aactaaaggc ttatttggta
    19021 ggtggtaaaa atattaaatc cacttttact tcagtaaaac aaaaaagccg gaaaaatgtt
    19081 ttccggctta taaaatattt cagatgtcag tcgcgataat ctttcaattg ttgttgcagt
    19141 cgcttacgtg ctaaaaaaat tcgacttttc accgttccta ttggcaaatg catggtttcg
    19201 gctatttctt catatttata tccctgcaaa tgcatggaaa agggaattct gtattcgtcg
    19261 ggaaatgctt caatgttgct gttaatttca ccgatggaat aggagccttc agggctgtca
    19321 aatccggaat tttgcggcaa attaaggtga tacatgtctt ctgttttatc aataactgtc
    19381 tgattgcgaa caatcttacg ataattgttg ataaatatat ttttcattat ggtcagcacc
    19441 caacctttga aattgacgtt tttggtaaat ttttccctat tgtccaatac tttcagggtg
    19501 gtatcctgca ataaatcttc tgcatcctct ctgttcaacg tgagtgaaaa agcgaaattc
    19561 cgcatattgt tttgtaaggc aattaattcg ttttcaaatt gatttggctt cataactttt
    19621 aatattaagt gtgtgaataa atttcacctc tccgacaaaa aaattcggca ggtaattttt
    19681 tattctaaaa cgaatctttc tgaaaaagtt tttgaatcga atagcatcaa atttatactt
    19741 ccattaaaaa cttaacaaag aactctttcg aaaatacttg ttctagattt tcacgacaaa
    19801 tatacagcat atttttaaat gtttcgtata tttggacaaa aatattcgat aatatgagaa
    19861 agttttatat attttacatt tttgtaacaa ttacaatgtg ttttataact gaaaatctga
    19921 atgcttcgaa taaaaaaacg actgtttttg ttatagaatt gaaaaaagat ataggtggta
    19981 cagcatgggt ttattgtcaa aaggcattta atcaagccaa agagaaaaat gccgatgtca
    20041 ttctgatgca catgaatact tatggaggcg aagtggtttt tgccgattcc attcgaacac
    20101 gaattttgaa cagcccgatt ccggtttatg tttttatcga taacaatgcc gcttctgccg
    20161 gagctctgat agccatagct tgcgacaaaa tttttatgcg acccggagcg gcaattggcg
    20221 cagcaacagt tgtaaatacc accggacagc aaatgcccga taaatatcag tcgtacatgc
    20281 gggcaaccat gcgtacaaca gccgaagctc acgggaaaga taccattatt cagggtaatg
    20341 ataccatttt tcaatggaag cgcgatccaa aaattgcaga agcaatggtg gacgataagg
    20401 tgtcgattcc cggtatcatt gaagcaggaa aaacattgac ttttacggca gaagaagctt
    20461 tgcaacacgg atattgtgat ggaattgcag aaagtattga tgaaattatt aaaaatcaca
    20521 ttactgaagc taatttcgag attattgttt ttcaaccgag tttttgggac aaagtgaaag
    20581 gatttttttc cagctccatt gtacgaagca ttttaattat gctaattatt ggaggtattt
    20641 attttgaact tcagactccg ggcataggat ttcccttggc aataagtatt cttgcagccg
    20701 tattgttttt tgttcctttg tatattgatg gtttggctgc caattgggaa attttgattt
    20761 ttttgctggg actggttcta ttggcattgg aaatatttgt aattcccggt ttcggtatag
    20821 ccggaatttc cggaattttg ttctctgtgg cgggtttaac gctcagttta ataaacaatg
    20881 tcaatttcaa ttttgatggg gttcaggcag gcacagttgg aaaatcgttg cttaccgttt
    20941 tggttggcat tacgttggga tttgttttga tattgtattt gagcaataaa atcggttcga
    21001 cgggaatttt caaaaaaatg gctctcaata cggcgctcga aaataaagaa ggttacatag
    21061 gcgtttcaat ggatgaattg cagctggtgg gtaaaacagg ttatgcttca acggttcttc
    21121 gcccttcagg aaaagtaaat gtcgaaggaa aagtttatga tgccgtttct acggaaggtt
    21181 ttattgaaaa aggagttcag gttcgcgtgc ttgaatatcg caccggtcaa atttatgttg
    21241 aaaagatata aatatttttc tctttctttt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nntcgcaccg gtcaaattta tgttgaaaag atataaatat ttttctcttt
    21421 ctttttcgta ttattgccta tctgtttgaa tttttcaaat agtatctcca aacagttgtg
    21481 taatttttcg gattatttta cttttgcagt ccatttaaat gaataagaaa ggtattttta
    21541 ttcagtaatt ttacaaataa tttcatgatt gtatataaat ttggtggtgc ttcggttcgt
    21601 tcggcagccg gaataaaaaa tctggcatca attgtttctg gcgtgaatga taatttattc
    21661 attgttgttt cggcgatggg aaaaacaacc aatgcgctcg aaggtgtatt ggaagatttt
    21721 atgcaggccg atcacgcttc tgctctggca aaatttcaac aaatagaaaa ttatcacaat
    21781 gaaattttag cagaactttt cccggatacc gaaaatcctg tcgctatttt gcagcctctt
    21841 tacgacgaga ttaccagtat gctgaaactt tcggttggtg acgattatga ccgatggtac
    21901 gataaaattg tatcctatgg tgaaataatt tcaacgcaaa ttgtttccaa attcctaaat
    21961 gaagccggta ttcaaaatgg ttggctggat atgcggaaac tgctgataac cgacagcaat
    22021 tttagggaag caaatgtcaa gatgaaagaa tcgcaacaaa aattgacgga agccgtaaat
    22081 ttcgaaaatt atccggttta tattggtcag ggttttatcg gagccaatac caaaggagat
    22141 ccgacaactc tgggacgtga aggttcggat tatacggctg ctgttgtggc caacttgctg
    22201 aatgcagaaa atttgactat atggaaagat gttccgggta ttttgaatgc cgatccacgt
    22261 ctttatcctc aggcaaagct tgttcccgaa ctgacttatt ccgaagcggt ggaattggct
    22321 tatagcgggg ctcaggtgat tcatcccaaa acgattaaac ctttgcaaaa taaaaatatt
    22381 ccgctttatg ttcgtccttt cgggaatccc tctgaaaagg gttctgttat taaagctcat
    22441 gtcgaaaaac cggccggtat tccgttttac agtttgttga aaaatcaagt gttggtaacc
    22501 attcgtccga tagatttttc ttttgtgctg gaagaaaatt tgccaaaagt tttttccatg
    22561 atcaataaac accggctaaa agtttcgatg attgaaagct cagccatcag catttcggta
    22621 gccattggca acagtcgata tttgccggct gctttggacg aactgtcgaa tgagtttgat
    22681 gtaagttaca acgaaaattt ggaattgctg actgtcagag gaatttcgga tgaaattgca
    22741 caggaagtta caaatcatcg agaagttttg cttatgcagc gtacgcgccg tattggtcgt
    22801 tttttgatga aagaaagata aattcttacc ttttggttat aaaaaaacag gacctgtgaa
    22861 atttttcact gtcctgtttt tcgtattaat tttcagattt gtttattttg ccaaatgttc
    22921 cagatttttt atattttcgg caatgtcgtt ttcagaaagg gaataattgg ttaagtcgcc
    22981 agcaaaatac tggtcataag cagtcatatc aaccattccg tgtccggaaa gattaaacaa
    23041 aatagttttt ggtgttcctt cctctttggc tttcagagcc tcatgaatag ccactgcaat
    23101 tgcgtgagcc gattccggcg ccggaacaat tccttcggtt tgtgcgaaaa gtacgccggc
    23161 tttgaaagaa tccaattgat caatatctac tgcttcgacc aagttgtctt ttttcaattg
    23221 gctgacaata gtgccggctc cgtgataacg cagaccacct gcgtgaatgt gaggcggagc
    23281 aaaattatgt cctaaggtaa acataggcaa aagtggagtg taaccaactt catctccgaa
    23341 gtcgtattca aaaacaccac gtgtcaattt cggacaagaa gccggttcgg cagcgataaa
    23401 acgtgttttt ttaccatctt taatattatg acgtaaaaat gggaaagata ttcctgagaa
    23461 attggaaccg ccaccaaaac agccaataac tatatcagga tattcgcctg ccatttccat
    23521 ctgcttttct gcttccagtc ctattacggt ttggtgcagt gagacatgat tcaaaacact
    23581 gccaagcgta tatttacagt ttggcgtatt catggcaagt tctatggctt cagaaatggc
    23641 agttcccaaa cttccgagat agttgggatg ttcggacaga atttttcgtc ctgcattggt
    23701 ggacatgctt ggagaagcaa ttacctgagc gccccaagtt tgcattagtg agcgacgata
    23761 aggtttttgt tcgtaactga tttttaccat gtaaacaaac aattccaaac cgaaagtttt
    23821 cgcagcaaaa gccaacgctg ttccccattg accggcgccg gtctcggtag tcaagtgggt
    23881 aacgccttcc tgtttacaat aataagcttg tggcaaagct gtgtttaatt tgtgggagcc
    23941 aacaggactg acactttcgt ttttaaaata aatgtgtgcg ggtgtgtcga gcgctttttc
    24001 caaatcataa gctcttacca gcggagtagg tcgataaatt ttatatttct cgcgtacttc
    24061 ttcgggtatt tcaatccatg catcggtctg atttaattcc tgatgcgaca attcttctgc
    24121 gaaaagtggg tacaattctt cttctttcat gggctgtttg gtagcaggat tcagaattgg
    24181 catgggcttg ttctgcattt ctgccacaat gttgtaccaa tgggttggaa gttccttttc
    24241 ggtaagaata aatttttttg ttttttccat tgcagtaatt ttaaaatttg agagacgata
    24301 aaattaatcg gattgatgtt gtgtgtgttg tcttaaaaaa aagccgaaaa ctggtgaaaa
    24361 gtatccggca atataaagcg gaagcgacga gattcgaact cgtggtacgg aaacccgtac
    24421 gtcagtttag caaactgatg gtttcagcca ctcacccaca cttccttgtg tctgcttttg
    24481 taatgcaaag ataagaaaga gttttcgatt atcaaagtca tttttactga acctttgttc
    24541 gggataattt taggggaaaa agaatttaac aattcaaatt gatttttaat gaattacatt
    24601 ttagaacgga taagtgtttt tttaagaaaa acatttactt ttgcaactca aatccaaacg
    24661 cattttttat gaaaaagaaa gtcaggaaaa ttttgtttgc agcgcttgcg gttccagtaa
    24721 ttgtggttat ttatttgacc tacgtttttt ttgcgcccaa tattttccca aaaaaagaaa
    24781 cagaaacttt tgtttgcatt cccgagaata cgagttttga agatgtcgtg aaacttatca
    24841 aaaatgaatc acgcgtgtgg gacagttatt ctatgcggtt aacggccaaa attttcgatt
    24901 acgataaaaa tatccgttcg gggcgctatg cgttaaaaaa aggaatgaac aattttgcat
    24961 taattcgaaa cttaaggagt gggagacagg ttccattgcg attgacattc aacaatatac
    25021 gaacaaaaga gcaattggcc ggtcgattgt cgtctcaact aatgaccgat tcactcactt
    25081 ttttaaaatt gctgaacaat tctcaatttt tgtcgcaata cgctttgaat cccgataatt
    25141 cggttgtgat atttataccg gatacttacg aagttttctg gaatattaaa cccaaaaaac
    25201 ttttcgatcg aatgtacaga gagtatttaa aattttggaa cgaagaacgc cggcagaaag
    25261 ctgcttccat accgctgact ccaatagaag tcagtatttt ggcatcaatt gtagaagaag
    25321 aatccaacaa atcgtatgaa cagcctatta ttgcaggagt ttatatcaat aggttaaaaa
    25381 ataaaatgcc cttgcaagcc gatcctacca taaaatttgc atggaaagat ttttctttga
    25441 aacgaatcaa aggtcaacat ttgcaaatca attcgccctt caacacctac aaatttcaag
    25501 gtttgccacc cggtcccatt cgattgcctt cgcctcaggt gattgatgcc gttttaaact
    25561 atacgcatca ttcctatctt tatatgtgtg ccaaagaaac gttgaatgga gaacataatt
    25621 tcgccaccac acatgctgag catttgatca atgctcgaaa atatcagcgg gcattgaatg
    25681 aaaggcagat ttttgaatga atttaaaata gtattgagcc gtttgtcata ttgaatttgt
    25741 agtttttaat caattggtta caatttatta agcgattttt tacccaactg ttgccacaat
    25801 ggaaaaatga tatatataat tctgttttct gatcataatt ttttacttgg ttgccgcatg
    25861 attgcattgt gtagctaatt aaaattattt caacctatct tttccctaag gcattttcaa
    25921 ttgtattgct atctatcggc tttaagacca cccacacgat aaaatcgtgc gagaatagga
    25981 tgggttttcg taaagtactt atccaattta tcagaaaatt tcgtaatgaa gagctttccc
    26041 ttacatttga aaaaaaaaag gaaggtgatt atttacccaa aaacattttt ctgacttttt
    26101 ccaaatcttc cggtgtatcg atgccataag attcggctgt tgtgaagcca acttttattc
    26161 ggtatccatt ttcgagccag cggagttgtt ccaatgattc tgctttttcg agcgatgaaa
    26221 cgggaagtga acagagttct ttcaatatct ctgttcgata cgcataaatt cccacatgaa
    26281 caaaatactg atgattcgaa atccaatgtt cgttggcagt atttcgttgg tatggaataa
    26341 cttgcctgct gaaatacagg gcttcattgt ttttattgag tactatttta ggtctgttgg
    26401 gattcagtat gtaatcgtat tcttcggttt ggtgaactat tttggcaagt gtagcaattt
    26461 gtgtttgttc atcttcaaaa cactgttgaa gcgtaacgat ttgnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnggttttc caccaatatc tgccagcggt tttcccggaa
    26641 aacgagtgga agcaaagcgg gccggaataa tgccgacaaa gcgaaaattt ctattagaat
    26701 ggagagtcat tggtcaaatg attttttata aagaaatttt atctcaattt ttccttgagt
    26761 ggtggtgaag cccgttgcta ttggtgtaat agtcccgatt attttttttc tttagcgaaa
    26821 aatctttttt tgttgtggct gtccatcgca ctgttcgcag caaaagcaac aggataagcg
    26881 gaaaactggc cggatggaaa acctgaagtc cagcagcata agctaccaaa gccagaaaaa
    26941 tcaataaaca gtttaaaact tgataataaa acacatacgg tttcattttg tgaaaccaaa
    27001 ggagaatgcc cagagcaaaa ttcagcggat tcagccacag aagattaaaa ttgtttttta
    27061 caagcggatg cgatgagaaa aaggcgagat aaaagacaat aattccacca aggccgctta
    27121 ctatataaag taggctatcg aacaaacgga aacttttttt attgttgaaa atagttatga
    27181 taattcccaa aattaaaaga aaagtacaaa caaatacagg ctgaaatata ggcaaaggtt
    27241 tagaatattt ttcttcctgt ttgggaatca atatcttatt ttcagctacc aggtggcgtt
    27301 ttgcaccatc tgcggctatt atgtgagctg cctgaaattc gtacatcaga ttttcaggta
    27361 aaaacatcga tacatttcgc gaaacgctct tgtctgcatc acgcccgaag attagatcaa
    27421 gaccaaattg catccaactg tttactccgg tgtattgagc tatcatttct ctgaaagtta
    27481 cgggctcgtt atataaatga tattcaatct ttccattaac tgattgtaca attttatctc
    27541 ttggccgggt agagcaattg tcataaacaa aattgtagcg gtagaatttg ttttcgggtt
    27601 cataatttat taaaagagag tgaagcagat ttcttttttc gttcaaagta aggttcagtt
    27661 tttgttccca aaccatcgaa tttctacttt tatattcttc caaaaagctt ttggtatcat
    27721 atacggcaag ctgataatcg gtatctccct taataaattt caggtagaag tttttggtgt
    27781 tgaaatcgaa tacgccaaaa ttgaaaacca catcgtagcc ggtaatcggg tcgttgacac
    27841 gaatagcagt atgtccgaat ttttcataca cttttttccc gggagaacaa gtgagcaaac
    27901 ttacttttgc cgaatcgctg aatgtgaaat tttgtgataa tattgggaat aatatgaaac
    27961 tgaggagaga aacgaatatt gctcttttca tgtattgcga ttggttttta tggatttcaa
    28021 atggcaaaag taataaattt ttcttttctc atagcttttt gttaaaagtt ttattcaatg
    28081 ttttcctcta cattttacag gtagtttcaa atgtttacaa ttcagaaatt ttttaatttt
    28141 tccccgttag aatttttatt cggaatagag aaatattttt attttacgtt ttggaattta
    28201 ttttaaaaaa ataattaaat ttgtgctgta taacaatgtt gtcaaacata ttttttcatt
    28261 tgaagcaatg accaaaaaac ctgcttatga actgaaaccc attgaagaag ttttcatggt
    28321 ttgggatgtg ctgacgcccg acgaaaggca atttgtaaga aacaactaca cggttcatca
    28381 tttcaagaaa aacgaactga tccaaagcga aggtgaaatt tctacccaca tgatgatttt
    28441 ggcggcgggt aaagcaaagg tttacaagga tggtattggt agccgaacgc aaattattcg
    28501 gatgttgaaa ccgggcgaaa atttcggtta tcgtgccatt atagccaatg agccaaacag
    28561 caccaatgta gcggcttttg aagcctcaac cgtttatatg ctcaaggccg atttgttttt
    28621 gtccattctc agacacaata ttgcagtttg ctatcgtttt cttgaagaat tggcttccga
    28681 cctggcagtt tccgatgcac gaactgtcaa tttgacacag aagcacattc gtggtcgttt
    28741 ggcagaagct ttgctgtttc tgaaaaaacg atacggagtg gaagaagatg gggctaccgt
    28801 caatatctat ttgtcgcgtg aggatttggc gaatttgtcg aatatgacca catccaatgc
    28861 tatacgcact ttatcgaatt ttaccaacga gcacatcatt gctgtggatg gaagaaaaat
    28921 taagattatt gatgaagata aactcagaaa aataagtaaa atgggataat ttttgttgtt
    28981 tttcgctttg tgattctttc tccaaattga aaaagtgata tttaattgac cattgttttt
    29041 atttatgaat gcaactcaaa ttgatactac ttataaggct attttgcgat ttgtgtcggc
    29101 gtcaaggctc aaacctgcat tcgacaggat aaaaatgatg attgacgatt tgcagtacgg
    29161 cgaattttcg gatcgtttta acgaattgga aaggaattac aaatttttac tcgattatta
    29221 tctcaagggt gtgcaagatc ccgaaaggaa atcggtttac agtaagttgg tcgcaaaact
    29281 tttgatactg cttgctgacg ttcgcgaaga gctgatgatg aaaaattctt caaatttcga
    29341 atattcacag aaaagatttt ttccatacac tcgtaaatat cagacagtca atgaactgtt
    29401 caatgctttg cagtatttcc acaagcagtc ggctttgctt tccgaaaata aggaacagta
    29461 caaaattgaa ttgagcagaa tgcgatccaa ttttgaagat gcgctggatg aactttttgg
    29521 cgtgttttgg ctggcaactc gctataccga agaagaaaac aatttgtaca agaaattgct
    29581 cgaagccgat gataaatact gggtggaaaa atccttgatg gtttcggccg taacgttgaa
    29641 tttgtggaga aaatttgacg aacaaaaaat aatgcttctt tttgacagtt gcgaaaacgc
    29701 cgatcaaaaa gttgttcagc gggcattagt cgggttaact tttatactgg ccaaatacaa
    29761 tatttatctg ccttatttcc cttccattcg taatcgtttg gttcttttag tcgatcaaaa
    29821 ccgtattgcc gaaaattttc agaatatcat tattcaaatt attgggacta ccgaaacaga
    29881 aaaaatttcg cggaaactca gggaagaaat tttgcctgaa atgatgaaaa tcaatccttt
    29941 gttgagagac aaaatggatg ctgaaaactt tttgaattcc gatgaatggg cagaagcaaa
    30001 tccggaatgg caggaaattt tcgatgaaag tggcttgtcg gataaactca aagaattgag
    30061 cgatttacaa ttggaaggag cggatgttta catgagtact ttttccatgc tgaaaaattt
    30121 tacatttttc tctcaatttt ctcattggtt cttgcctttc gatactgaaa attcggcagt
    30181 caatcagttg ttcgagtcgg atgaaaaaac cattttgtcg gcttttgtca acagcaatgt
    30241 catgtgcaat tcagacaaat attcattttg cctgagtgtg ttacaaatgc ccgaaagcca
    30301 acgaaatatg cttaaacatt cttttaaaat ggaatcggat caaatggacg aaatggaaga
    30361 agaaatgtcg atactttcgc ccgaaacaat tacaaaaaac atttccaaac agtacattca
    30421 ggatttgttc cgtttcttta aacttcagcc tcacaaggaa gattttgaag atatgttttc
    30481 ctattctctg actttacatc gcacctggct gtttgatatt ttgtcttcta ttcccggttt
    30541 ttcagaaagt gtagcggaat attatttttc taaagggtat tatttgcagg cactggagct
    30601 ttatgaaaaa attcagtcgg agagtcagcc ttccgctgcg ttgtatcaga aaatgggtta
    30661 tgcgtatcag cagacttcgc aattgcaaaa ggcactggat gcctatttga aagccgatat
    30721 gattcaaccg gacgatggct ggactattcg aaaaattgcg ctttgttatc ggttgctggg
    30781 aaattatgaa aaagcgttgg cattttatca acatgccgat tttctgaaac ccggtcagta
    30841 cagtacgcaa atacatatcg ctcaatgtta ttttgaactc ggaaaattta aagatgcgct
    30901 ggcaatttat tataaactgg atgccgaaaa tgtggacgac ataaaagtgt ggaaagcgat
    30961 tgcaaaatgc aatttttttg acggaaatct ggataaagcc gattattatt cagaaaaaat
    31021 tattttggct gaacccaatg cttacgatta tttgctttcc ggtcatatta gctggtgccg
    31081 acgtcagttg tctgcagcag ccaatgctta tcgcaaggct ttagaaatgt tgcagaataa
    31141 atgggaagtt tttcaggatt tgtttaatga agataaggac aggttgattt cgtttggtat
    31201 tcgggaagaa gaattgccgc tcatgatgga tgcattgcta tattacgaga atgaatagaa
    31261 atggttggag aacattttaa aatttatatc ctattcccgc atcaataaat tccgcttttt
    31321 gcaaatgggt tttcaatccc aaagatagga ggaaatgttc gtccaaagca tattttaata
    31381 cagcttttgt gtaaaaccaa ccatcttcct tttcaatatt atatttgtaa atcaaaggtt
    31441 tgtttaaagt tccgtttttt gcttcttcgt aaggttcgag atttttcacc ggattataaa
    31501 ggtacaaacc aaaatggaaa cctgttgtga gtctgccaac taaaagctcg tgtcgaaaag
    31561 aagcgccaat tctaagtttg tattttaact cgtcggtagt gagataagtt cttttgaaag
    31621 ctgatttacc attaaaaacg ccatcgtaaa acaaatctgc gccccatccc attctgtaaa
    31681 aattggtcat ggttcggtat gctgctatat tgaaagtgcc tatgggatag gttttgtcgt
    31741 ctttgtaata aagttgtcgt ataccgccgg taagagccat ttcaatttcg attttatgcg
    31801 gcaggtctgg taatggttga ggttttggtg ggatataacg ctcgtaattt ggaaaatatt
    31861 taattcccaa atacatatcc aacatattga ttcctgaatt tggttgaaca atacttccgt
    31921 tggaagtatg attccatccc cagttggagg taatgctcca tccttttgtc aaaggaattt
    31981 ccatgttgag attggctgta agatagacat tcacatgtga tccgatagcg gcattgcttt
    32041 tgttgagata tacgctgcca tcaggatgat aagccgtagc attgtagaaa gtttttgtta
    32101 cgtaactcag ccctccgccg gctttgaaag ccatattaaa atgttgctga cgaataatag
    32161 ggaatttcaa atatggatat agggctacag cataacccaa tgtgtcggga tgactgaaat
    32221 ccaaaaaagc agtggaaatt ccaatggaag gaaaattaag atactgatgc cacgcttttt
    32281 ctcccattgt ttgccattct attgaaaatt ctccgccttt gagcggtcct ttgaccagat
    32341 tttcgagatg cttgttgtgc ttgagaattt caccgtaaat aaaatctgca ctcagagaat
    32401 aatttttaaa cgtttgcgca aagccaaagg agtagattgc gaaaaacatt ggcaataaca
    32461 ggaatttttt tttatttctg cccataaatg taatttttcc tttgacaatt tttggttacc
    32521 tttgaaagcg taaaattaac aaatatttta atcgagggaa ttattttagt cttcatcagt
    32581 aaataattta aacaaaaacg ccgattggta tgatttctat tttcaaaaaa gaactcggaa
    32641 gttttttcag cagtgctaca ggatatttgg ttattggcat ttttttggcc ttgacaggat
    32701 tgtttttgtg ggttattccc ggcgaattta atattcttga atcaggttat gccaatgtgg
    32761 atgggttgtt ttatctggcg ccatggcttt tcttgttttt gtgtcctgcc attaccatgc
    32821 gatcttttgc cgaagaaaaa aaatcgggaa cttgggaaat actcataacc aaacctttga
    32881 gcatcacaga actggttttg ggaaaatttc tggccggttg gattttggtt ttaattgctt
    32941 tatttccaac cgttttatat tatttctcgg tttatttttt ggccgaacca gtgggaaata
    33001 ttgatgccgg agctttctgg ggctcgttta tagggttaat ttttctgtcg ggtgtttaca
    33061 tagccattgg aattttttcg tcatcgcttt caaacaatca aattatttcg tttgttgtgt
    33121 cagtgttgct gtgttttatt ttcttttacg gtttcgactt attgggaagt tttttctctt
    33181 ccggagctgt gattgatttt gttcaaaatc ttggaataaa cgctcattac aagtctatga
    33241 gtagaggagt gttggatatg cgtgatgtgg gatatttctt gtttttgggg ggattgtttt
    33301 tgggaataat ggtatggaaa aattccaaat cggacaagta agcgctgatt ttgaaagaat
    33361 attttttatt gtcgattttt aaaatttttg ttgttagaaa aattaatgag gaagcatgaa
    33421 taaaaatttg attttgattt caaatgatga tgggatagat gcaaaaggga taaaaatttt
    33481 aacgaaatta atgatgcagt tgggagatgt agtggtggta gctcccgata ttccgcgttc
    33541 ggctcagtca agtgctttga cggttacgca gcctattcgt tacagaaaaa tagaagaaga
    33601 ggaaggaatg gttcgatatt cctgtaccgg aacgccaacc gattgtgtaa aaattgcact
    33661 gaatgaaatt ctgaatcgta aacccgattt attggtttca ggaatcaatc atggctccaa
    33721 ttctgccatc aatgtcatat attcgggcac tatgggtgct gtttttgaag gttgtgagaa
    33781 cggaattcct tccatcgggt tttcgttggc cagccatttt cccgatcctg attttagttt
    33841 ttttgaacct cacgtattga aaattgcaca aattgtgatg gaaaaaggtc ttccagaaag
    33901 tgtttgtctg aatgtgaatg ctccctgcgg agaaattcac ggtgtgcgtg ttgcgcgtca
    33961 atgcaagggg tactggacaa aggagtttgc tgcaagaacc gatcccgccg gcagggctta
    34021 ttattggctt accggatatt ttgaaaatat ggaacccgat gccgaggata ccgacgaatg
    34081 ggctatcagt catggtttta tttccattgt tcccgataaa attgatttga cagcttatga
    34141 tgcaatggac ttgatcaggg aatggaattt gtaaattctt atgccgtgaa acgtctcaac
    34201 catttttata ttggcttttt agtgggtttg atactgccct gcgttttcgt ttggctctat
    34261 ataacaagct tctatcctgt cgatatatct ttttttgaaa ttttgaaacg tctttatcca
    34321 ggcgttttgt tggggaagtt gctgctgttg tccattgttc ccgatttgct gatggctttt
    34381 gttttttata aaaatgatgc ttttcgtctt acttcgggca ccattgtggg cggattgcct
    34441 tttttaattg caagtctttt tatgctgtaa ttgtccgatt atttgtatac atagctaaaa
    34501 ttattgattt tatattttca tgaaatattt tttgattgcg ggtgaagcat caggcgattt
    34561 gcatgcgtcg catttgatgc acgaactgaa aaagcaggat ctgcaagccg aattttgctt
    34621 tttgggcggt gatttaatgt ggaccgaagg tggagaaatg ataagacact atcggcaaat
    34681 ggcttttatg ggatttattg ctgtgttgaa aaacctttcg gttatttctg taaatatcaa
    34741 agatggcaag caggcgcttc tcaaatttca gcctgatgtg ctgatactgg tcgattatcc
    34801 gagtttcaac ctcagaatgg ccgaatttgc aaaaaagaaa ttgaatattc ccgtatatta
    34861 ttatatttct ccaaaaatat gggcatggaa agagtttaga attaaaaaaa tcagacgtta
    34921 cgtagataga atgtttacga tttttccttt cgaaacggag ttttatgcaa aacatcgtta
    34981 tccggtcgaa tatgttggta atcctacagt ggatgcggtg gaagagttta agaaagctta
    35041 tcggggaaat ttccaaactt tccgtcaatc caataatttg gtcgataaac ctgtgatagc
    35101 actattggcc ggaagtcggc gacaggaaat caaatcctgt ttaccgcgca tggttgaggc
    35161 tgcaatgcag tttcccgatt atcagcctgt ggttgccgga gcaccgggtg ttgagaaaga
    35221 tttttatttt tctgttttaa aagatacttc agttgctgtg gtttttggtc aaacttatga
    35281 gttgcttttt catgccgaag ttgccattgt caattcagga acagctactc tcgaaactgc
    35341 tttaatcggc actccgcaag tggttgttta tcatgttgcc tttgggaaag tggcttattg
    35401 gttgaaaaaa ctttttatta aagtgaaata tatttcttta gtcaatctga ttgccggaaa
    35461 ggaagtggtg atggagctgt atgctcattt gtttacggta gaaaatgtga aaaatgaatt
    35521 aaatagaatt cttacagatg cgcattacag gcaaaatatg aaaaatgagt ataaaaaagt
    35581 ttctgagata ttgggagaac ccggagccgc aagccgagct gcaaaaaaaa tcttcgagtt
    35641 gttgcattcc gacaagtctg tccttacccc atgacacaag ccatagccaa actgtaatat
    35701 ttggagttga cgctttcgct tctcgaggtc agcactaccg gtttttgcgt tccctgaaga
    35761 agacctgcca tttcggcctt tgcaaaaacc attaatcctt tatagaaagc atttgcacat
    35821 tcaaaacttg ggaaaatcaa aatatctgca tcgccatgaa tgggtgaaga tacttttttg
    35881 atacttcctc tttcagggtc taaagcaaga aaaatatcga gcgggccatc cataataaca
    35941 tcgccaaatt cacccattcg ccatttctcc attatgtcga gataatcctg catgtattga
    36001 attttcggat tggctttttc cgttgcatgg attaaagcta tttttggtcg ggttattcca
    36061 aatttgtaag ctgtttccgt tacgtatttt atcatggcag tccgttgttc tacagtagga
    36121 gaggggatga ctgccggatc ggtaaaaaac agcagttttt ggtaatcggg tatttctaag
    36181 gctgcattgt aggtaataat ttttcctttt tctaccaaac cgttttcttt attcagaatt
    36241 gcccgcaaaa tcacgtcggt gtttaccaat cctttcatta atatgtcggc ttcgcctgtt
    36301 ctgaccattc ttaccgcttc gcgtgtagct tcttccactt ccggaatatc gataatgtgc
    36361 acataaggag ttagtttgtg ttcaaaaaag tgtggagatt ctatcatggc caagtcgcca
    36421 atcaggaaag cttcaacaaa accgttatcc gaagctctta gtaccgcttc caatgtatgt
    36481 ggatctactg catttgctac tgcaacgcgt tttctgttgt ttaaaaattt taaatgggct
    36541 atcagttcag cgaatgaaga aatagattcc atacaatgat ttttctacaa acttacataa
    36601 aaatttggaa tttattcatt tttttcaaaa tacctgttcg tattttttct gattcatacc
    36661 gatttttttg taatgccttt ttagaggaaa cataattaca aaaaaaaaga ttggatttgc
    36721 cttgaaacaa atccaatctt aaaaaattga aatttagctt ttgttttata ggaaaaatca
    36781 atcaattatc tcaaatcctg tgtacggaat caaagctttt ggaatgcgaa taccttcggg
    36841 agtttgatta ttttccagca aggcagccac aattcgaggc aaagccaaag cactgccgtt
    36901 gagggtgtga caaagttcaa ttttcttatc cgcgcttctg taacggcact tcaatcgatt
    36961 ggcctggtaa ctttcaaagt tggaaaccga actaacttcc agccaacgtt gttgggcagc
    37021 cgaataaact tcgatgtcga aggtaagagc ggaagtaaaa ctcatgtctc ccccgcaaag
    37081 tcggagtgtt cgccaaggta attcgagctt ttcaaccaat ccttccacat aatgaaccat
    37141 ttcgtaaaat gtttcatacg atttttcggg atgttgaatt tgaacaattt ccaccttatc
    37201 aaattgatgg agacgattta atcctctgac atcttttccg tacgaaccgg cttcgcgtcg
    37261 aaaacatgca gaataagcca cattttttat cggcaattcg ttttcgttga gaatcatacc
    37321 tctgtaaatg ttggtaacgg gaacttctgc ggtcggaatc aaataaagat tgtcttcctg
    37381 acagaaatac atttgacctt ccttatcggg aagctgtccg gtggcataag ccgaagcttc
    37441 gttaacaaca taaggtggct gaacttccat ataaccggct tcgcgagcat tgtcaaggaa
    37501 aaagttgata agagcgcgtt gaagtcgggc acattttccc ttataaaggg gaaatccggc
    37561 tcccgtaatt ttaacgccgg tttcaaaatc tataatgtcg tatttttttg ccaagtccca
    37621 atgaggcaag gcatctgttg gcaatgaggg aatttttcct ccggtacgtt ccacctgatt
    37681 gtcttctgca agttttcctt cgggaacgct ttcgtggggt aaattgggaa tttgcaccag
    37741 aatttcgttc agttgggttt caatctggtt ttgtttttct tcaagaagtt taatattttc
    37801 cttcagctca agggtttttt gcttcaaagt ctcggcatcc gatgttttac cttgtttata
    37861 aaggatccca atttctttgg atattttatt aatttcggct gcgatggaat cggcgtcata
    37921 ttgagttttt tttctttcgg cgttaagctc cagaactttt gaaattaact cttttccatc
    37981 aaaatgtttc tttttcagtc gtttcagaac atcttcggaa ttttcggcaa tatatttaag
    38041 tgtcagcatt ataaaaacaa ttttttattt tagcagttaa aagacgaccg aaaaacttcg
    38101 gccaaaaaat tgaggtttga gaaatattta ataaaaaaat ctcctacaat tttaccgttg
    38161 ggtaaattat caggagatta tcatgtgttt acaagtctct tgcattgaaa gagactgaaa
    38221 tttttcaatt catttcggga ttgatggaaa cataagagcg attaccgact ttcttgcgga
    38281 attcaacttt tccatctacc agagcataca aagtatggtc tttccccatt ccgatatttt
    38341 ttccgggatg atgaaccgta cctctttgac gaacaatgat attaccggct ttggcaaact
    38401 gtccgccata aatttttact ccaagtcgct tgctttccga ttcacgacca ttttttgagc
    38461 ttcctactcc ttttttatgt gccattttta gtgaattttt aaaattaagc tacaatttct
    38521 ttgatcaata attcagtgaa atcctgacgg tgtccggtgc gttttctata tccttttcta
    38581 cgttttttgt gaaaaacaat tactttttca cctcttacgt gtcctactac atcacaaacc
    38641 acttttgctc cttccacgca tggcgcaccg acagctattg aaccatcgtt gtccaccagt
    38701 aatactttgt cgaactccac ctgtgagcct atttcggctt cttccattcg atgtacatac
    38761 agctttttgc ctgcctgtac cttaaattgt tgtcctaaaa tttctacgat tgcgtacatt
    38821 ttattcgttt ttttgttaaa tcagtaaggt gagatgccta aggcagtgct tcttaagaac
    38881 cttttctgaa tgaaggtgca aatgtacaat ttttttttgg tttttcaatc tttttctcaa
    38941 tatttttccg gttacttttt tctttgattt taaaaacttg aaagatttta aagaaactcg
    39001 aatttttttg gtttattgga cgaatttgca attacagttt gaaaaaaaaa ttgagtagga
    39061 aagttttaat aatcatgtgt cgtaggataa attgaaaatt atccattatt tttgttgttg
    39121 aaactgtgaa gatatatgga atatcaattg accgattttt tgccggttac gcgaaaagag
    39181 cttgaattaa gagcatggga agatgtggat gtggtttttt ttaccggtga tgcctatgtg
    39241 gatcatcctg cttttgccaa tgccttgata ggaaggctga tggagtcgtt ggggctccgt
    39301 gtagctattg ttcctcagcc caattggaga gacgatttgc gtgattttaa aaaaatgggg
    39361 cgaccacgca tgttttttgc agtttcggcc ggcaatatgg attctatggt caatcactac
    39421 acggccaaca agcgtttgcg ttccaacgat gcttattctc ccgacggaaa acccggcttc
    39481 cgtccagatt atgccacagt ggtttattgt aatattttaa aaaaacttta tcctgatgtt
    39541 ccaattctta taggcggaat agaagcttca ctgcgacgac tgacccatta cgactattgg
    39601 tcggatcagt tgaaacaaag tattttatgt gatgctcaag ccgatttact tatttatgga
    39661 atgggagaat tgccgctgac cgaattaatc cgtcagttaa aaaacgggaa acaatttcat
    39721 gaattgaccg atattcccca aactgtttat ttgtcggata acattcccca agacaatttg
    39781 tggaatacaa tcgagttgcc ttcctttgaa acttgtttgc acgacagaaa aaaatatgct
    39841 tctgcattca aaacaataga agaacagtcg aaccgatatc aggcagatcg gttaattcaa
    39901 aagaccgagg acaaatgggt agttgtaaat ccaccttatc ctccaatgat tgacgcacaa
    39961 cttgatgctt cttatgaatt gccttacacg cggatgcctc atcccaaata caaagggaaa
    40021 accattccgg cttacgaaat gataaaattt tccgtcaata ttcacagagg ctgttttgga
    40081 ggttgttctt tttgtaccat ctctgcccat caaggaaaat ttattgtgtc aaggtcaaaa
    40141 aaatctattt tgaatgaagt agagaaaatt aagcttatgc ctgattttaa aggttattta
    40201 agtgatttgg gcggaccttc ggccaatatg tatcaaatga aagggaaaaa catagaaatt
    40261 tgcaaaaaat gtaaaaaacc ttcctgtatt tttcctaaag tttgtttcaa cctcaacacc
    40321 gatcatcata aattgctgga tatttatcgt tcggttgatg ccttgcaagg aattaaaaaa
    40381 tctttcattg ggagcggtat ccgttacgat ttacttttgc acgaatcggc tgatgagttg
    40441 actaaaaaaa gtcaatccga atacatccga gaactgattc ttcaccatgt ttcaggtcgc
    40501 ctgaaagtgg ctcctgagca tacttcggac cgtgtgttgg caataatgag aaagccttct
    40561 tttcaatatt tcattcggtt caagacccag ttcgatagaa tcaacaagga agcaaactta
    40621 aatcagcaaa ttattccgta ttttatttcc agccatcccg gttgtacaaa ggaagatatg
    40681 gccgaactgg ccgttataac gaaaaatttg gattttaaac tggaacagat tcaggatttt
    40741 actccgacac ctatgacgct ggctaccgaa atttattatt ccggatttca tccctattct
    40801 ttgcagccgg tttatgttcc caaaaccaaa gaagagaaac tcgagcagcg aaaatttttc
    40861 ttttggtaca aacctgaata tcgtagtcag ataaaacgcg atttattgat gatgcaacgt
    40921 cccgatttgc tcaagaaatt gtttcaaaat gaaagaaaat aatttatcgt atataacagt
    40981 tatttttttg ttcccgtttt ttttgaaaag tgtataaaaa tcagtaactt tgtaagttga
    41041 tagcaggttg gtttgtcgct ttccaaattt tttggaaaga ggaaagtccg ggcaacacag
    41101 agcgccatac ttcctaacgg gaagctgtcc gcaagggcag agtagcgtaa cagaaaacaa
    41161 ccgccttgtt tgaggtaagg gtgaaaaggt gaggcaagag ctcaccggta agagtggcaa
    41221 cacttttagc cgtacgcctt atgggttgca agatcatgta tatcgacgca tgtaggatgg
    41281 ctcgtccgat gtcgaggggt agatcgctta aagcaagtgg cgacatttgc ttcagataaa
    41341 tgacaaacac tttgcttttg caaagtacaa aacccggctt acaaacctgc tatttttttt
    41401 aaaaaagtgg ctgatttttc aatgatattt tttgtggtaa atctcaaatt cacaaaattg
    41461 taaatcatca ttcatctgct tatggcctct ccgataaatt tttttgtccg catttacaat
    41521 ttttttgttt ccgacatttg gaaaattgcc gaaaacgaac tcacgcgtac ccgccgtttt
    41581 ttttatcatt tgataaaaat aattctttat gctattcgtg gttattccac tgataaatta
    41641 gccataaaag cttctgctct gacttattcc atcctttttg cagtggtacc tgtaattgcc
    41701 attatcattg caataggacg tggctttgga atggaaacac tgattgaaga tgcgttaaaa
    41761 aacactttta tagcgcaggc gaatcttgtg ccggtagtta tggactttgt taaaaaatac
    41821 ttggaaagca ttcagggcgg cgtatttctg ggcgtgggta ttgtaatttt gctttcggcg
    41881 gtagtgaatt ttttcatgca ggtggaagat actttcaaca gcatttggca ggtaaagaaa
    41941 tcgcgttcct ttcttcgtca gtttacaacc tatttttcca ttattcttat tttaccgctt
    42001 tttatagtgc tttccagcgg attttcaatt tttgtaaaca ctaccatttc tcaaattccc
    42061 ggttttcaga ttttgaatcc gcttttaaaa gttggagcaa atttattacc ctacttaatc
    42121 aattggttga tttttacttt tatgtactta tttatcccca atacccaagt acgatttatc
    42181 aatgctttga ttgccggaat tattgcgggg acagcttttc agctatttca aattctgtat
    42241 atcaccggac aaatcaattt gtcgcgttac aatgctgttt atggcggttt tgctgcgatt
    42301 cccctgctca ttctttggat tcgcatttct tgccttatcg ttcttttggg agcagaattg
    42361 acttatgctt ctcaaaacat tcgtaattat gaatcagaat tggatactca aaatatcagc
    42421 ataagaaaca gaaagcttat ttctttatac attacctatc ttgttatcaa acaatttgaa
    42481 ttgcaaaaac cacccttatc ttccgagcag atagcttccg attttaaatt accaattcgt
    42541 ttggtgaatc agattctcaa tcaattagtc aatatatcgg tattaataga agttttcaac
    42601 gataaaaata aatctaaaag ttaccagcct gcgatggata tcaatcaatt aacagtagga
    42661 ttgttgtttg aaaagttgga atcttacgga acgggaataa ctcttttaaa gaataacgaa
    42721 aaacttaaaa atttctggga aaagacgtcc gctgttttag ctgcaggtag taaagaatct
    42781 tctaatgaga ttttaattaa ggatttgtaa attatttgtt tacaacatta atgtattgaa
    42841 acaaaaaagg gcaagctccc tacatcgcac cgctacaact ttctaccctt gctgcggtca
    42901 agccctgggg gagtttgaaa ggagctggtc gtgtaggact taccccgttg caaaggtacg
    42961 attttttatc atatttccaa atatctctgt attttttcct cagcatacag cacatgacaa
    43021 aaaaaatttg ctcgtgtggc aaaactttaa atcaacatca ttcctctggt catccctacg
    43081 ggattttttt gttc
//
LOCUS       DAOTAX010000011        40489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_801_length_40489_cov_31.473265, whole genome shotgun sequence.
ACCESSION   DAOTAX010000011 DAOTAX010000000
VERSION     DAOTAX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 40489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40489
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_40489_cov_31.473265"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..1638)
                     /locus_tag="PLM04_02380"
     CDS             complement(388..1638)
                     /locus_tag="PLM04_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01959.1"
                     /translation="MNINQQIIDNLHSPEELEKIYREHKREFSDFFKNNKGNYDSELY
                     RFWQVRLAAETSHSRHKALKSDIIFLMLVSLFSGLLLKLPDFFAQLDKNSFYLRNLSL
                     ILFNALILYAFWQNKLFKRHEMLIYSISIVALAIYVNLLPVDKSDAVRLALLHVPVLL
                     WYLFGWVLVSFDRNNLNKRIGYIRYSGEYLVMTGLILIGGALLTALTINMFSAIGMNI
                     ENFWMEYVVIFGGAAMPVVSAFLIHIYPDVTGKITPVLARIFSPLALVTLAVYLVFLL
                     MGNSNIMKDRNLLMVLNVVLFAVLVLVVFSISEIDQSKKKNAGVFVLFLLTLLAILVN
                     VVALTAIVSRLFNGFTPNRTVVLGFNVLIFINLILLLPKLFRSFSHPEMLEEVEQSVA
                     RYLNVYFLWTLMVVFVLPVLFRFR"
     gene            2036..2944
                     /locus_tag="PLM04_02385"
     CDS             2036..2944
                     /locus_tag="PLM04_02385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HON01960.1"
                     /translation="MNKQTHKRTPLPSTKKGQEMKVSENALLLDFLLKSMNHKSRNSV
                     KSLLKHGQIVVNGKTITRYDYPLKLGDIITVGNHQSIVEKNIPQMRILYEDDDIIVID
                     KEAGLLTITTGNGIDITAVSLLKDYVQKQSPYNKIYTVHRLDQMTSGVLVFAKNSEAQ
                     HQLRDNWHNMVTKRTYVAVVEGKMDESAGKISSWLTENKKNYMVYSSPVDNGGKLAIT
                     RYKVLQTSDQFSLLELELETGRKNQIRVHLKDLGHPIVGDRKYGASTSIGRIALHARV
                     LEFYHPVSGEIMHFETPVPHLFLRLL"
     gene            complement(2964..3602)
                     /locus_tag="PLM04_02390"
     CDS             complement(2964..3602)
                     /locus_tag="PLM04_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HON01961.1"
                     /translation="MKILKNIVFDFGGVLIDWNPRYLYRNVFETEDEMEYFLEHICPY
                     DWNLMQDAGRLLAEATLSKQKEHPEYSNEIALYYGRWIEMIGGIFEENVKLLKPLKEK
                     YNLYGLTNWSAETLPLAQAKYDFFDLFDGIVVSGAEKIVKPDPELYRILIDRYRINPQ
                     ETLFIDDNPQNIQTAERLGFHTLYLTPQINLEQELKNRGIIDCWKKEFRSEK"
     gene            complement(3778..4191)
                     /locus_tag="PLM04_02395"
     CDS             complement(3778..4191)
                     /locus_tag="PLM04_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HON01962.1"
                     /translation="MKSEVRIDKWLWAVRLFKTRSLAAEACKKGKILIHGQPVKASHS
                     IRVGDVVGVKRNPVLFSYQVLALTENRLNAKLLPEYLLDVTTPDQRELFELGKLNSSG
                     RIRGSGRPTKKERRDLEDFVEPFFLDDDEDGWEDL"
     gene            complement(4204..4767)
                     /gene="pth"
                     /locus_tag="PLM04_02400"
     CDS             complement(4204..4767)
                     /gene="pth"
                     /locus_tag="PLM04_02400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HON01963.1"
                     /translation="MKFLIVGLGNIGSEYENTRHNIGFRILDAFAEASNVFFTDQRYG
                     AVSEVKLKGHTLILLKPSTFVNLSGNAVRYWMQKEKIDVEHLLVVVDDIALPFGTIRM
                     KPKGSDAGHNGLKNIQEILGHNHYARLRFGIGNDFPRGRQVEYVLGKWTEEEAAQLPE
                     RIEKAIEVIKSFCLIGVEKTMALYNNK"
     gene            complement(4888..5460)
                     /locus_tag="PLM04_02405"
     CDS             complement(4888..5460)
                     /locus_tag="PLM04_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HON01964.1"
                     /translation="MKEFDLKGALRNGFGKKASKLERSSEKVPCVLYGNGENVHFTVS
                     EVDVLKLIVNPDVFVVNLDIEGKKCKAIVKEVQFHPVTDKILHIDFFMISEAKPVVVE
                     LPVKLEGLAEGVKAGGRLSLEMRKLKVKGIYTKLPEKVVIDVSSLGLGKTIQVGSLSF
                     NDFEILNAKNAVVAQVKVTRASYAAAAGGK"
     gene            5849..6376
                     /gene="infC"
                     /locus_tag="PLM04_02410"
                     /pseudo
     CDS             5849..6376
                     /gene="infC"
                     /locus_tag="PLM04_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            6434..6631
                     /gene="rpmI"
                     /locus_tag="PLM04_02415"
     CDS             6434..6631
                     /gene="rpmI"
                     /locus_tag="PLM04_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HON01965.1"
                     /translation="MPKKKTNSGAKKRFTLTGTGKIKRKHAFKSHILTKKTKKQKRNL
                     THSALVHKANLESVKELLNLK"
     gene            6718..7068
                     /gene="rplT"
                     /locus_tag="PLM04_02420"
     CDS             6718..7068
                     /gene="rplT"
                     /locus_tag="PLM04_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HON01966.1"
                     /translation="MPRSVNHVASRKRRKRILNLTKGYFGSRKNVWTVAKNTWEKGLL
                     YAYRDRKAKKRSFRALWIQRINAAARLEGSSYSRLMGALHKSGIVINRKVLADLAMNH
                     PEAFKAIVQKAKNA"
     gene            complement(7305..8303)
                     /locus_tag="PLM04_02425"
     CDS             complement(7305..8303)
                     /locus_tag="PLM04_02425"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HON01967.1"
                     /translation="MKTKVILFDAKSYDEIFFKEKNQQYGFDIKFQKSHLSKDNAILT
                     KGADVVCIFVNAEVDAEVIDILVENGVKLIALRSAGYNNVDLKAAKGKIKVVRVPAYS
                     PHSIAEHTIALMLTLNRKIHKAYTRTREGNFALEGLMGFEMNGKTAGIIGTGKIGKIV
                     IQILKSMGMNVLAYDLYPDTQFAETNGIRYTQLDELYGLSDVISLHCPLTKETEYLIN
                     EKSIHKMKEGVMIINTGRGKLIKTKALIDALKSKKIGYAGLDVYEEEGDYFYEDHSDI
                     VIDDDILARLLTFNNVIVTSHQAFFTKEALSEIARITLQNIQDFVDGKPLVNEISA"
     gene            complement(8434..10482)
                     /locus_tag="PLM04_02430"
     CDS             complement(8434..10482)
                     /locus_tag="PLM04_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HON01968.1"
                     /translation="MKLKVNKKKFETVLKLLLAFALVIIIVELYPHESTFNYQFEEGK
                     PWSYELLTAPFDFPIYKSNEQIEKEKKEILKNFTPYFNLDTTVMHIQINKFKIDSSRS
                     GNISPDLTTFIEEKLKSIYSKGIISNDDYNNLQKWNVKKIYRILPGRISQLATLNEIY
                     TPKTAYEEIVKSNQENIKELDINNYLVENLRYDSITSETAKNELLKNLSLTVGMVQSG
                     ERIIDKGEIVSPEAYAILRSLQIENENRKSAFEQTAIVLTGKILLVIGLITLLILYIY
                     LFRPRILDNFNNLLFLSLLIILIVGLTSLTIRYSSLSYYIVPFALLPIIVRVFYDSRT
                     ALFVHIIVIFIVSFMTDNPFQFALLQITAGMVAVSSLKDMTQRSQLAQTALYIFLSYT
                     VVYLSLELMTEGNISKIDWYPIISFAVSSLFLLFAYLLIYIFEKMFGLLSAVTLVELT
                     NVNSDLLMKFAEVAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGKMKNPE
                     FFTENQIGGKNPLLSMQYEEAAKLIIEHVANGVEIARKNHLPNQIINFITTHHGTSKT
                     KYFYVSFINDNPGIEPPEDVFSYPGPLPNTKETAILMMADAVEARSRTLSEYTEESID
                     QMVESIINTQIAEGQLKDAPISFRDVETVKKVLKEKIKSIFHSRITYPEINKNLSTNV
                     "
     gene            complement(11323..12879)
                     /gene="dnaB"
                     /locus_tag="PLM04_02435"
     CDS             complement(11323..12879)
                     /gene="dnaB"
                     /locus_tag="PLM04_02435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HON01969.1"
                     /translation="MAEKKTYNKKQNSAPTVIAPNEMGKLPPQALELEEAVLGAIMIE
                     KDAYFLVSEILKPACFYKLAHQKIYEAIMTLALHQEPIDMHTVTEQLRRDGTLEEIGG
                     PYYITLLTAKVSSAAHLEYHAHIILQKFLARELIRISSEIQTKAFDDKTDVDDLIEES
                     EEMLFEIAQRNLEKEVIQIKPVIDEAIQRIQAAANKQGTSGVPSGFHSLDKITSGWQK
                     SDLIIIAARPAMGKTAFVLSMAKNMAVDYNVPVGVFSLEMSSVQLANRLIMNVCEIDG
                     VKIKNGDLSPYEWDKLDKNIKDLIDAPVFIDDTPSLSISELRSKARRLVKEHKVQCIF
                     IDYLQLMNASEMRFSSREQEVSTISRSLKSLAKELDIPIIALSQLNRSVEGRSGVDGK
                     RPQLSDLRESGAIEQDADVVCFIHRPEYYNLTTDKDGNSMIGIAEIIVAKHRNGATDT
                     IHLKFKKEFAKFLNLDDIDSDDIFNENYQVISSKMNVPEGNFYENTSGSPALDNLLKN
                     NNNNNGEVPF"
     gene            13158..14891
                     /gene="lysS"
                     /locus_tag="PLM04_02440"
     CDS             13158..14891
                     /gene="lysS"
                     /locus_tag="PLM04_02440"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HON01970.1"
                     /translation="MNIELSDQEQYRRQSLTELRNLGIEPYPAALYPTNAFSTDIKSN
                     FDENNPQRMEVCVAGRIMSRRIMGKASFVELQDAKGRIQIYITRDDINTEAQPEMYNT
                     VFKKLLDIGDFIGIKGYVFRTQTGEISVHAKELTVLSKSLRPLPIVKYKDGVAYDAFD
                     DPEQRYRQRYVDLVVNEGVKDIFLKRTKVYQSMREFFNTRGYVEVETPILQPIPGGAA
                     ARPFITHHNALDMPLYLRIADELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTA
                     MEIYVAYKDYFWMMDFTEQMIEKICLDVNGTTEVQVGDKTISLKTPYKRISMIDAIKE
                     NTGIDIEGMDENDLRKVCEDLQIDVDETMGKGKLIDKLFGEKCEANYVQPTFITDYPL
                     EMSPLCKRHRNNPELTERFELLVNGKELANAYSELNDPIDQLERFQEQLRLSEKGDDE
                     AMFIDMDFVRALEYGMPPTSGMGIGMDRLVMLLTGQQAIQEVLLFPLMKPEKVQKVDT
                     KEKFMELDIPEAWAEVLQKMGIKQVDQLKDLNPNKLHQDICGFNKKNKLALTNPSKEE
                     VSQWIDNAAKK"
     gene            15269..16234
                     /locus_tag="PLM04_02445"
     CDS             15269..16234
                     /locus_tag="PLM04_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HON01971.1"
                     /translation="MFEIVFELSSLADTAVFYGINNSNMQLIKNLHPNLRIVARGNVI
                     KIIGNQQDADKLIVNLQKLEKFSIDNNQLTEQNIIELVKGENPKEISFQNLIMHGVGG
                     KSIFARTKNQQRLVEEFEKNDLLFAIGPAGSGKTYTAIALAVRALKNKQIKKIILSRP
                     AVEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMITPLKLKEYMENGTIQIAPLAFMRG
                     RTLSDAVVILDEAQNSTIMQIKMFLTRMGLNTKMIVTGDITQIDLPRDQKSGLIDALN
                     LLGNIKGIARIDFDVNDIVRHRLVQQIVQAYDNRAMIERQKNTKE"
     gene            16308..17261
                     /locus_tag="PLM04_02450"
     CDS             16308..17261
                     /locus_tag="PLM04_02450"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HON01972.1"
                     /translation="MNTLVRTDFHFEGQTNVYHGKVRDVYFIQDDLLVMVATDRISAF
                     DVILPKGIPYKGQILNQIAAKFLDLTADIVLNWKLACPDPMVTVGLKCEGFPIEMIVR
                     GYLCGSAWRAYKNGVREICGVKLPDNMRENQRFETPIITPTTKAELGTHDEDISRQEI
                     LAKGLLTSEEYDLLEKYALALFERGTQVAAERGLILVDTKYEFGKRNGNIYLIDEIHT
                     PDSSRYFYAEGYEERFEKGEPQKQLSKEFVREWLMENGFQGKEGQKVPEMTDEIVNSI
                     SERYIELYEQITGESFQKTDTRNLAERIESNVMKYLQSLKK"
     gene            complement(17356..18909)
                     /locus_tag="PLM04_02455"
     CDS             complement(17356..18909)
                     /locus_tag="PLM04_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HON01973.1"
                     /translation="MFKNHPKGLIAASLANMGERFGFYTMMAILSLFLMAKYGLDETK
                     AGLIYSVFYFSIYILAFVGGLIADKTRNYKRTILWGLILMSIGYFLVALPTPTPVPQN
                     RYGFFLGLTCFGLLTIALGNGFFKGNLQALVGQMYDNPKYEKMRDSGFSLFYMFINVG
                     AIFAPFAAIGVRNWWLHSHGFAYNPDLPALCHDQLNGIITPEAAQRMHELAVNAGYTG
                     SNMTSFADQYLNLFTTGFHYAFMVAIGAMFVSLIIFVTNKKSFPQPRNLSSTATVTLD
                     QNGKEMDAKEIKQRLYALLAVFAVVIFFWFSFHQNGLTLTFFAKDYTNLSKVSVNLGF
                     TTLQGAEIFQSINPFFVVFLTPLVMALFGYLRSKGKEPSTPRKIAIGMGIAALAYVVM
                     TLGSLGLPSKEEITLRGGLPDAERVTPLLLLGTYFILTVAELFISPLGISFVSKVAPQ
                     KYQGIMQGGWLAATALGNQLLFLGALMYKNIPIWMTWSVFVGACLISMFTMLAMLKWL
                     ERITGEKQG"
     gene            complement(19057..19587)
                     /locus_tag="PLM04_02460"
     CDS             complement(19057..19587)
                     /locus_tag="PLM04_02460"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HON01974.1"
                     /translation="MQSNKDNRIFLIGYMGAGKTTIGQQLATRLGYKFLDMDHVIEEK
                     MQQSVSQIFSEKGEEEFRRLERECLEEISTEQNVVVATGGGAPCFYDNMERMNAYGLT
                     VYLCLSPEQLQERLELSHKNKRPLLAQREGKELLDFIRQNLSEREPFYRKAALVVSGT
                     DEEMFQQILSFLSSHS"
     gene            complement(19766..20308)
                     /locus_tag="PLM04_02465"
     CDS             complement(19766..20308)
                     /locus_tag="PLM04_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01975.1"
                     /translation="MKKQFVLLSFMAVVLLTGCNIDGNSNYTPAIQFLAFPYTQHGDT
                     LNIKTTNESGVYLLDSIQVGDTVFFQLGLNAYTNNLVSFRLTKTVDSAGDFLFPPVAS
                     LDSVFSHTNSDYGQGKFVFLSGFNAVYFPFHFAAKSATNDEILQFSLVSDAQFDDVYG
                     SNVTTFKLKTPIKPAAVVEE"
     gene            complement(20388..20460)
                     /locus_tag="PLM04_02470"
     tRNA            complement(20388..20460)
                     /locus_tag="PLM04_02470"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20425..20427),aa:Pro,seq:cgg)
     gene            20663..21391
                     /locus_tag="PLM04_02475"
     CDS             20663..21391
                     /locus_tag="PLM04_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HON01976.1"
                     /translation="MFTEAIQNYLQRHDYKSFYPKAVFFDMDGVLIDSMKNHSRAWMQ
                     AMKESNLPFTEEDAYMHEGCIGATTINDTFMKHLNREATEEEQKQIYSAKSRIFESLE
                     PIEKMPFAYELLKKLKSEEMKIYVVTGSGQPKLIDSLNTLFPEIFTKETMITGFDVKE
                     GKPSPEPYLLALKRSGFQPWETIVVENAPMGILSASKAGLFTLVVNTGPFDDDFLYSY
                     GADLVLDSMEHLYNEWDNFRKSWI"
     gene            21357..22424
                     /locus_tag="PLM04_02480"
     CDS             21357..22424
                     /locus_tag="PLM04_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HON01977.1"
                     /translation="MNGTISVNRGFEMKKTGLELFLSPVFLSLILFFSSTVIFAGETD
                     SASFHPQIEGTIRAKYEYNTSLNASRFQVRNARFSVNGNFNSLVSYKTEIDLSDEGQT
                     KMLDAYIRLQPQKWFTFTIGQQKIPFSTDNLRPPHLLYFANRSFIGKQLTALRDVGTT
                     LSFVNNKTLPFEFITGLYNGEGLYNQKIWQTSFSYAFRLVLHPCENLHFSLNHNSIRP
                     EEMRMNLYDLGGILQLKNWHLETELFYKTYQQNFFPTTKGFFVFSSYDIYLHNKESIK
                     IIRPLIRYDSMTDNNKGYKSENGSYESDDMARSRITGGITLCLDKPFLNDIRLNYEQY
                     FYKKGIQNNDNKFVVEFVLRF"
     gene            22497..23810
                     /gene="hisD"
                     /locus_tag="PLM04_02485"
     CDS             22497..23810
                     /gene="hisD"
                     /locus_tag="PLM04_02485"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HON01978.1"
                     /translation="MQVIKYPAREFWSTLLSRPAIDRKSLFEKVGSIVSEVKQNGDKA
                     VKKYTLQFDKVGIEELEVSQEEIEEASLQIPNQLKEAIEMCRRNIWKFHSAQQHDLPE
                     IQTTPGVVCWQKAMPIEKVGLYVPGGTAPLFSTVLMLGVPAQIAECKEIVLCTPPDKE
                     GKIHPAVLYAAKVAGIHRIFKIGGIQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     IALDGIAIDLPAGPSEVMVIADESARPCFLAADLLSQAEHSVDTQSILITVSENLVEP
                     VLLEIEEQLNRIPKKEIALKALENGKIIVLQSLEEAVAMANEYAPEHLIISTRYSLGV
                     ASNISNAGSVFLGHYTPESAGDYASGTNHTLPTNGYAKAYSGVNLDSFMRKVTFQQIS
                     REGLSNLSNAIILMAENEGLYAHSNAVKVRLDKEESEEKPENRLC"
     gene            23886..24920
                     /gene="hisC"
                     /locus_tag="PLM04_02490"
     CDS             23886..24920
                     /gene="hisC"
                     /locus_tag="PLM04_02490"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HON01979.1"
                     /translation="MKINELVRENIRNMQPYSCARSEFKGEASVYLDANENPYNSPFN
                     RYPDPLQTELKKEISKIKKVPVENIFLGNGSDEAIDLLYRAFCEPKIHNVVAIEPSYG
                     MYRVCAEINNVEYRPVLLDENFDVEAYKLMDASNDKTKIIWLCSPNNPTGNSLNFSEI
                     NKLLSWFRGIVVIDEAYIDFSTKKSLLNYLTLFPQLIVLQTMSKAWGNAGIRLGMAFA
                     DPEIIGILNKIKYPYNINYLTQQHALQALQNPMQVEKWVALILEEKEKLIKELQKLKL
                     VEKIYPSDANFILVKVENANAIYRFLIQNGIVVRNRSNMPLCNNCIRITVGTPEENKA
                     LIDTLKKFHV"
     gene            complement(24980..25053)
                     /locus_tag="PLM04_02495"
     tRNA            complement(24980..25053)
                     /locus_tag="PLM04_02495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25017..25019),aa:Met,seq:cat)
     gene            complement(25387..26397)
                     /locus_tag="PLM04_02500"
     CDS             complement(25387..26397)
                     /locus_tag="PLM04_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HON01980.1"
                     /translation="MEETKYMPADFKTDQQPRWCPGCGDYAIFNALVKTMAELGVSPH
                     ETVIISGIGCSSRLPYYTNTYGFHTIHGRGACVATGVKVANPALTVWQVTGDGDCLAI
                     GGNHFIHSVRRNVDINVLLFNNQIYGLTKGQYSPTTKKGFVTKSSPYGTVERPFRTAE
                     LTFGAGGTFFGRLLDVDLKTSQMVMKAAALHKGTSVVECLVNCVIFNDGAHGWLAEKE
                     TRDDRIVVLEHGKPMIFGKNKDKGLILEKSELKVVTIGENGITDKDILVHDAKCKDTF
                     LHTKLALMEGPEMPIAMGIIRDVEEETYEMAMENQINEIKKQTSVKSFDELIDACEQW
                     EI"
     gene            complement(26431..28275)
                     /locus_tag="PLM04_02505"
     CDS             complement(26431..28275)
                     /locus_tag="PLM04_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HON01981.1"
                     /translation="MNKAQTIQLDNIVIRFSGDSGDGMQLTGNLFAQLSAELGNEIST
                     FTDFPSEIRAPQGTLGGVSGFQVHVGANKIYTPGDEIDVLVTLNPAALKVNAKNLKKG
                     SIVIFDLDSFQPSDLKKANFSTDNPFEELSLSDTIQLIPVPLTTLTKSSLEDFGFDNK
                     TVLRSKNMLALGLVCWLFNRPLDKAIHFLENKFRKKTQILQANIKALTDGYNYGENLH
                     LTVSNFVIEKSHEMPKGKYTSINGNKATAWGLIAAAEKAGLQLFLGSYPITPATDIMQ
                     ELALQKGIGVKTVQAEDEIAGITMAIGASFAGCLAATSTSGPGMSLKTEGIGLAVMAE
                     LPLVIVDVMRSGPSTGMPTKTEQGDLLQALYGRSGESPLAVLAAPTPDSCFHYAFMAS
                     KIALENMTPVILLTDAFIGNGSSLWRLPKLADLPEIHPHFVPSNMENYKVTTRNEDTK
                     IRYWSIPGMKGFQHCNGGLEKDYTTGNLSTDPENHEKMVLTRKQKVDNIVNVIPELEI
                     EGNTDADLLVVGWGGTYGHLKSAVDNLNNKGYKIALAHFTYLFPLPANTENVIGKYEK
                     IVVCELNTGQFASYLRSKIPGKTFTQFNKIQGQPFKTEELENHFISLL"
     gene            complement(28336..29742)
                     /gene="asnS"
                     /locus_tag="PLM04_02510"
     CDS             complement(28336..29742)
                     /gene="asnS"
                     /locus_tag="PLM04_02510"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HON01982.1"
                     /translation="MELMKRTKIVDALKKIDFGEDICVKGWVRTRRGNKNVSFIELND
                     GSTVHNIQIVADNNKLGIDFLKPITTGACIGVTGKLAQSLGKGQSVEIQAEDIVIYGT
                     ADPETYPLQKKEHTMEFLREIAHLRPRTNTFGAVFRIRHHMAMAIHTFFHERGFFYFH
                     TPIITGSDCEGAGQLFQVTTLNLSDLPKAENGCIDYSNDFFGKQTGLTVSGQLEGELG
                     ALALGAIYTFGPTFRAENSNTPRHLSEFWMIEPEVAFNELEENMQLAEDFVKYCVRWV
                     LEKCSDDLEFLNNRYDPELIDRLKFVIENDFVRLTYSEGIKILEDAMAKGQKFEFPVG
                     WGIDLQSEHERYLVEKHFKKPVILTDYPKNIKAFYMKLNDDGKTVRAMDVLFPKIGEI
                     IGGSQREDDYEKLKARAKEMEVPDKDIWWYLETRQFGNAPHSGFGLGFERFLLFVTGM
                     SNIRDVIPFPRTPKNADF"
     gene            complement(29932..31446)
                     /locus_tag="PLM04_02515"
     CDS             complement(29932..31446)
                     /locus_tag="PLM04_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HON01983.1"
                     /translation="MRVKVLIKVSTFCLALLFLGCAGQSNKEGYSRVTGWKYNDEKGA
                     GFAVRKDFKTNVPPGMVEIEGGSFTIGEKGEVVNAPRNSRRRRITVSSFYMDQYEIRN
                     VDWREYTHWMNLVFGKTAPKLVEKTYPNGKVWREELAYNEPYIEHYFTHPAFDQYPIV
                     GVTWEQAMDYCSWRTDRVNELALVKAGIIAPPDFASLKNETNFDNIINNFVFNTQKYL
                     KQASYQPPVGKNPKKDLTGEIRKVDMSDGILLVSDFRLPTEAEWEYAAYGLKAGKDGF
                     IPEGKIYPWNGHQVRNPSRKQLGQMMANFVRGRGDMMGTAGSLNDRATITAPVNSYYP
                     NDFGLYNMAGNVNEWVLDVYRATSFEDVAEYNSFRGNMYLTPIVEKEDEVGNKIFKID
                     SLGRIATTVLEGDDVRNYKDGDLTSKISTDFQLYADSSDIAAITNMKKIDPTDVLAPQ
                     ISDKTRVYKGGSWKDRIYWLNPSTRRYLDQDQCSNDIGFRCAMSKMGNIEPVKR"
     gene            31807..32658
                     /gene="folP"
                     /locus_tag="PLM04_02520"
     CDS             31807..32658
                     /gene="folP"
                     /locus_tag="PLM04_02520"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HON01984.1"
                     /translation="MFLNLNGRLVDVSSPVVMGIVNVTPDSFYTGSRFLSEKSILTSV
                     DNILSNGGTIVDVGAYSTRPSAESVSVEEELKRLSRALEIILKQFPNVILSVDTFRSK
                     VARYVIENFHVAMINDIGGGTLDELMFETVADLRVAYVLMHMRGTPQTMQTLTHYENN
                     VVSEVFSFLQKGLAQLHLLGVKDVIVDPGFGFAKTLEQNYELLHKLHYLKELNAPILV
                     GLSRKSMIYNLLRLSPEEALTGTIAANMLSLIGGASILRVHDVKEALQTIRIFEQYQN
                     FSTFIND"
     gene            32670..33482
                     /gene="cdaA"
                     /locus_tag="PLM04_02525"
     CDS             32670..33482
                     /gene="cdaA"
                     /locus_tag="PLM04_02525"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HON01985.1"
                     /translation="MFFQIEFKDIVDIFLVAFLLYEIYKLLRNSGAINIFIGILTFIV
                     CWFFVSYVFKMQLLGEIMDTVVNVGAFALIVLFQDEIRRFFTQIGTKRENRFLSKMKK
                     IFGESEYDKKETDFDLLQVVLACRNMSRTNTGCLIVITRRNALEFYVQSGELIEAKIN
                     SRLIESIFFKNSPLHDGALIISRHTIKAAGCILPVSQNQSIPKRLGLRHRAALGITEH
                     TDAVAIVVSEETGHISWAVNGNLTHNVKPEELEYFLNTQLQGSNKVEEEKIK"
     gene            complement(33551..34543)
                     /locus_tag="PLM04_02530"
     CDS             complement(33551..34543)
                     /locus_tag="PLM04_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HON01986.1"
                     /translation="MVHYTELGLVNSRELFKKAIEGKYAIPAFNFNNMEQLQAIISAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYNIPIVLHLDHGDTFELCK
                     SCIESGFSSVMIDGSHLPYEENIALTKKVVEYAHQFDVTVEGELGVLAGIEDDVVAEH
                     HTYTDPDQVVDFVTRTNVDSLAISIGTSHGATKFKPEQCTRDENGILIPPPLRFDILE
                     EIEKRIPGFPIVLHGASSVPHEYVEKINKYGGALKDAIGVPEDQLRRAATSAVCKINI
                     DSDGRLAMTAAVREVFATKPAEFDPRKYLGPARDELKKLYMHKTVNVLGSAGKA"
     gene            34779..35198
                     /gene="mscL"
                     /locus_tag="PLM04_02535"
     CDS             34779..35198
                     /gene="mscL"
                     /locus_tag="PLM04_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HON01987.1"
                     /translation="MKLWKEFKEFAMRGSVIDLAIGVIIGSAFGKIVSSLVADVIMPP
                     IGLLISGINFTDLKLVLKPAEMVNDVQTPAVTLNYGNFLQVAFDFLIVAFVIFLLIKG
                     INNFLKKKEEKPVPEATAQPSKEEILLTEIRDILKEK"
     gene            complement(35268..35549)
                     /locus_tag="PLM04_02540"
     CDS             complement(35268..35549)
                     /locus_tag="PLM04_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01988.1"
                     /translation="MQTINQLFQLLLKDIQEKMNDEFDRNFERNTFFAEAWNPNKHNS
                     IGLLMIWTGALRKSLRSEKIDIYSIWLQSLLPYAELHNCGETFNRTKRN"
     gene            36400..37941
                     /locus_tag="PLM04_02545"
     CDS             36400..37941
                     /locus_tag="PLM04_02545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HON01989.1"
                     /translation="MTHQNGLDVKTLENWLWEAACKIRGPIDAPKYKDYILPLIFLKR
                     LSDVFEDEINRLSQNYGSREIVEQFLSNDHNLVRFYLPPETRWEAIAKHTTGVGEYLT
                     NAVRKIARENPKLQSVIDIVDFNATAGGQRIISDEVLKDLINVLGKYRLGLDDVEPDI
                     LGRAYEYLLRKFAEGSGQSAGEFYTPSEVAVLMSYILDPKPGNEIYDPCCGSGGLLIK
                     AFLRSKEKFDDNPSFEPVRFYGQEILHATYAMAKMNAFIHDMEAEIALGDTMNRPAFL
                     TSAGALRKFDLVTANPMWNQDFSQTVYENDPYNRFVFGFPPSNSADWGWIQHMFASLN
                     DAGKMAVVLDTGAVSRGSGNVGSNRERDIRKEFVERDLVEAVILLPENLFYNTTAPGI
                     ILVINKAKPADRKEHLLLINAAHLFEKGRPKNYLPDESISRIADIYLQWKEEEGISKI
                     ITNQEAARNDYNLSPSRYVAQNGEDDTLPPEDAVIQLREAEEERQKADEKLKAILQEM
                     GLWKN"
     gene            38046..38639
                     /locus_tag="PLM04_02550"
     CDS             38046..38639
                     /locus_tag="PLM04_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON01990.1"
                     /translation="MTYNPNNHHRRSIRLHGYDYSQPGIYFITLCTYNRECLFGEILN
                     GEMRLNEFGKIANQCWLEIPNHFPQTQLDEYIIMPDHIHGIIVLNDFVGVQNVGTKNV
                     GTKIVGAKNFSPLPMPTPEPTREQNITPFRSPSKTIGSIIRGFKIGVTKWFRQNTDIY
                     VVWQRNFYEHIIRNEVELNRIRQYIIDNPKKWKTDEN"
     gene            38738..39991
                     /locus_tag="PLM04_02555"
     CDS             38738..39991
                     /locus_tag="PLM04_02555"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HON01991.1"
                     /translation="MIMNSINDEILLPQGYKMTELGPLPEEWEVVKLGEVCKVDWGNT
                     SLTKKLYTANGYPAFSAAGNDGFLDFYEHDGEAVILSAIGARSGKCFYASGKWTAIKN
                     TIVIKSNNETKINLLFLYFFVNNENYWHKSGSGQPFISIGVAQKQKLPLPPLPEQQKM
                     AAVLSAVQEAKEKTHAVIDATKALKKSMMKHLFTYGPVSPEEAANVPLKETEIGKVPE
                     DWEVVRLGDIAETYSGGTPRRDHKEYFEGKIGWLKSGELNDNYIYSTQELISEEGLRK
                     SSAQYVESNTLLIALYGATAGKVGLAKRKFTINQAICAVRPNNKFWTEFYFYYFQTIR
                     DKLLSMRFGCAQPNLNQQIVKNTILPLPPLHVQQKIASILSAIDEKIQAEEAKEKALE
                     DLFRSLLSHLMSGKIRVKDLGIGNC"
     gene            40071..>40489
                     /locus_tag="PLM04_02560"
     CDS             40071..>40489
                     /locus_tag="PLM04_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HON01992.1"
                     /translation="MAKLGGERYSVQQPIIQYVQEPSAAYVSQDGLTMFLKLGWEYVK
                     PDDALRLKGGEAGMVFREIFIQQLQRLNPDFLDHLMAKELIKKIETVPANIEGNFLAW
                     EYLKGLRTVFVPTEKRERNVTFIDAENMDRNTFHVTD"
BASE COUNT        13026 a         7749 c         7497 g        12217 t
ORIGIN      
        1 caattcggtc tcccagcatg acgcacaacg tcttatttac gccataaata tacaaatttc
       61 gtaaatgcaa aatttaaaac atgcgtaaac cacttttaag tttttgctta ttattaatgg
      121 taaaaagcat gatatcaata ggataggtta ttcaaaaaaa ttacatttac gaataggtat
      181 tgaccttcct taattgtgag gttcgtatgt catttcatat ttgttttctg aatcagctat
      241 ctcagcaaaa tcttattcct tctttttctt tctctacctg cctactattt ttttaataat
      301 ttcacaaata tacacaacaa ttctattcac acaaaacaat gatttaaaaa aattaatttt
      361 gaaatttcga ccttgatatt gtccaactca ccgaaacctg aacaacaccg gcaatacaaa
      421 taccaccatc agtgtccaga gaaaatacac gttcaaatat cgggcaaccg actgttccac
      481 ttcttcgagc atttcaggat gagaaaagga acgaaacagt tttggcaaca aaagaatcaa
      541 attaatgaaa atcaatacgt tgaaacctaa aacgacggtt ctgtttggtg tgaatccgtt
      601 gaaaagtcgg gagacaatgg cggttaaggc taccacattg accaaaattg ccaaaagagt
      661 cagcagaaac aacacaaaaa cgcctgcatt tttctttttc gattgatcga tttccgaaat
      721 cgaaaaaaca accaacacca aaacggcaaa aagaacgaca ttcagtacca ttaataaatt
      781 tctgtctttc ataatattac tgttccccat caaaagaaaa accaaataaa cagccagcgt
      841 aaccaatgcg agcggtgaaa aaatgcgggc aagaaccggt gtaattttcc cggtaacatc
      901 cggataaata tgtatcagaa aagccgaaac aacaggcatg gcagctccgc caaaaattac
      961 aacatattcc atccaaaaat tttcgatatt cattccaatg gcagaaaaca tgttgatagt
     1021 caaagcggtg agcaatgccc ctcctatcag gattaatcct gtcatcacca aatactctcc
     1081 actgtaacga atataaccga ttcttttgtt caagttattg cggtcaaacg aaacaagcac
     1141 ccagccaaac agataccaga gcaataccgg aacatgcaac aaggcaagac gaacagcatc
     1201 acttttgtcc accggaagca gatttacata tattgccaaa gcaactatcg aaattgaata
     1261 aatcaacatt tcatgcctct tgaacagttt attttgccag aaagcataaa gaatcaaagc
     1321 attgaaaaga attaacgaaa ggttacgcaa ataaaaagaa tttttatcca actgtgcaaa
     1381 aaaatccggt agttttaaaa gcaatccact gaatagtgaa acgagcatca aaaaaataat
     1441 atccgatttc agcgctttat ggcgactatg tgaggtttct gccgccaaac gtacttgcca
     1501 aaaccgataa agctccgaat cgtagtttcc cttgttgttt ttaaaaaaat ccgaaaattc
     1561 ccttttgtgt tctctgtaaa ttttttccaa ctcttccgga gaatgcagat tgtcgataat
     1621 ttgctgattt atattcattt aagtaatttg aatgaaatgg ttttttgtac aaaacagaaa
     1681 aaattgataa ttaatttact gtattgtcgc cttgtgcaaa ttccaattat tttcacacag
     1741 tggttgacta tttaagaaat aaacgtacac ttttccctta aattttaaaa aaaatcaaaa
     1801 atatactcga ataatttttt ggcaccttca ttcttcaagg gaaatgcaat gccataattt
     1861 ttgtaatttt gcgacaggat taaaactgtg attgcattga aaaaataata gaaaaagtcg
     1921 gaattatcta aaatgatttc gatcgacgtt gcatcattct tcaaaataat cgtcaggcaa
     1981 tggctaactg aatggctcat gggtttataa aagattacga aaacaactta tcgaagtgaa
     2041 caaacaaacg cataaaagga ctcctcttcc atcaacgaaa aagggacaag aaatgaaagt
     2101 gagcgaaaat gcccttttgc tcgatttttt gctgaaaagc atgaaccata agagccgaaa
     2161 ttcggtaaaa tcattactca aacatggtca aatagttgtg aacggcaaaa ccattacgcg
     2221 atatgattat ccgttaaaat tgggcgatat aattacagtt ggcaatcatc aaagtatcgt
     2281 tgagaagaat ataccccaaa tgcgcattct ttatgaagat gatgacatca tcgtcatcga
     2341 caaagaggcc ggactgctta ccatcaccac cggaaatgga atagatatta cggcagtttc
     2401 gttgcttaag gattatgtac aaaaacaatc tccctacaat aaaatttaca cggttcatcg
     2461 gctcgatcaa atgacttcgg gagtgctggt ttttgctaaa aacagcgaag ctcagcatca
     2521 gttgcgcgac aattggcaca atatggttac caaacgcact tatgtggctg ttgtagaggg
     2581 gaaaatggat gaatcggcgg gaaaaatctc gagttggctg accgaaaaca aaaaaaacta
     2641 catggtgtat tccagtcctg tggataatgg gggaaagctg gctatcacaa gatataaagt
     2701 tttgcaaaca tccgaccaat tttcgctatt ggaattggaa ctcgaaacag gacgaaaaaa
     2761 tcagataagg gttcatctca aagatcttgg gcatcccatc gttggcgacc gcaaatatgg
     2821 agcatccaca tctataggcc gcatcgctct gcatgcccgc gtgttggaat tttatcaccc
     2881 tgtttcgggt gagatcatgc atttcgaaac acctgtaccc catctttttt tacgtttgct
     2941 atgaaggaag cttccgctta taattatttt tctgagcgga attccttctt ccaacaatcg
     3001 ataatgcctc tatttttcag ttcttgttct aaattgattt gaggcgtcaa gtacaacgta
     3061 tgaaatccga gccgttcggc tgtttggata ttttgaggat tatcgtcgat gaacagtgtt
     3121 tcctgaggat tgattcgata acgatcgatc aatatccgat agagttccgg atctggtttt
     3181 acaatttttt ccgcacccga tacaacaatt ccgtcaaaca aatcaaaaaa atcgtattta
     3241 gcctgagcaa gcggaagcgt ttcggccgac cagttcgtga gtccatacaa attgtatttt
     3301 tctttcagcg gcttcagtaa tttcacattc tcttcgaaaa ttccacctat catttcaatc
     3361 catcgaccat aatataacgc aatttcgttg ctgtattcgg gatgttcttt ttgtttcgac
     3421 agcgtagctt cggcgagcaa acggccggca tcctgcatca gattccagtc gtatggacaa
     3481 atgtgttcga gaaaatattc catttcatct tcagtctcga aaacgtttcg atacaaataa
     3541 cgaggattcc agtcaataag tacacctcca aagtcgaaaa cgatattttt gagaatcttc
     3601 atatgacgta agtttgaatt ttatacaaaa gtaaatctta aaaattgaaa acagaattca
     3661 gttttcattc gccctaacag cagggtttat taaactataa aaattggtat attagtgaat
     3721 aggaaagaat aaaaagcaac ctattaaacg gattgaagaa atttcaggta agtatgttta
     3781 caaatcttcc cagccatctt cgtcatcgtc gaggaagaaa ggttcaacaa aatcttccaa
     3841 atcgcgacgt tccttttttg taggacgtcc actaccgcgt attcttcctg aactattcag
     3901 ttttccgagt tcgaacaatt cgcgttgatc gggagtagta acatcaagca gatactcggg
     3961 cagcaatttg gcattcaatc tgttttcagt caaggccaga acttggtaag aaaaaagtac
     4021 gggatttcgt ttcacaccaa ccacatcgcc aacacgaatc gaatgcgaag ctttcaccgg
     4081 ttgcccgtga atcagaattt tccctttttt acaggcttcg gcagccagcg aacgggtttt
     4141 aaacagcctg acagcccaca gccatttgtc gatgcgcact tccgatttca taaacagagg
     4201 aaattatttg ttgttgtaca atgccatcgt tttttcaacc ccaatcaaac agaaactttt
     4261 tattacttct atggcttttt caattctctc gggcaattgg gccgcttctt cttccgtcca
     4321 ttttccaagt acatattcaa cttgtctgcc acgagggaaa tcattgccta ttccgaaacg
     4381 caatcgggca tagtgattat gacccagtat ttcctgaata tttttcaagc cattgtgtcc
     4441 ggcatccgaa cctttgggtt tcatacgaat tgtgccaaac ggaagagcaa tatcgtccac
     4501 cactaccaat aaatgttcta catcgatttt ttccttttgc atccagtatc tcacggcatt
     4561 tccgctcaga ttgacaaaag tggaaggttt caacaaaatg agcgtatgac cttttaattt
     4621 cacttcactg actgctccat aacgttggtc ggtaaaaaaa acattggacg cctcagcgaa
     4681 agcgtccaat atcctaaaac ctatgttgtg gcgggtattt tcgtattcac ttccaatatt
     4741 gcccaaacct acaattaaaa acttcatata caattatttt caaatcggtt cattattgaa
     4801 cttctgaaaa atttacgaac aaatcggttg ttacatttgc cgaattcttt cttttaaaag
     4861 aaaaacgaca aaccattaaa acaaaaatta ttttccgcct gctgcagcag cataagaagc
     4921 tctggtaacc ttaacctgtg caacaacggc attttttgca ttcagaattt caaaatcgtt
     4981 aaaggaaaga cttccaacct gaatggtttt gcccaatcca agcgaagaaa catcaataac
     5041 tactttttca ggaagtttgg tataaattcc cttcactttc aattttctca tttcaagact
     5101 caaacgtcca ccggctttca caccttcagc caaaccttcc agtttaacag gcaactcgac
     5161 aaccacaggt ttagcttcag aaatcataaa gaagtcgata tgcaaaattt tgtccgttac
     5221 cgggtgaaat tgaacttcct taacaatagc tttacatttt ttcccttcta tatcgaggtt
     5281 taccacaaat acatcaggat taacaatcaa ctttaaaaca tccacttcag aaactgtgaa
     5341 atgaacattt tctccgtttc cgtacaaaac acacggaact ttttcagaac ttctttccag
     5401 cttgctggct tttttcccaa acccgttacg taaggcccct tttaaatcaa attctttcat
     5461 tttaacttta aattaatgtg ttactcaaaa tttaagcatt aaaaaaacat gtgattagta
     5521 gcttaatttc ccatcacact ttcttttttc aaaaaaagtg ctgcaaaggt agttaatttt
     5581 ttgatgtaaa acaagataaa aactacccaa attattcgaa taaacctatc aattacaatc
     5641 aaaataaaga acaaaaaact ggtcattatt attaatttat acattattag aaagttagat
     5701 ttgatttatc agcactctgt cggtaaaaat attcgacaga ataatggaaa attaaaaaaa
     5761 aacatttata tttgcagtct taaaatttta aaataatacg tttgaaaatt aataacgagg
     5821 aggaacagtt atagctaaac aattatctgt gaaacaacaa ggtaagtcaa cagaacagct
     5881 ccatagaatt aacgaaaata ttaagggagt aaaagaagtt cgtctggttg gcgaaaatgt
     5941 cgaaccggga atttatccta tacagcgagc tttagaaata gccgaagaac aaaatttgga
     6001 cttggttgaa atttcagcca aagcagatcc tcctgtatgc aaggtgattg attatcagaa
     6061 atttctatat cagctcaaaa agcgtgaaaa ggaactgaaa gccaaaacgg ctaaagtggt
     6121 agtaaaggaa attcgttttg gaccacaaac cgacgagcac gattacaact tcaagctgaa
     6181 acacgcacaa aactttctga aagaaggttg caaagtgaaa gcttatgtat ttttcaaggg
     6241 tcgttccatt cttttcaaag aacaggcaga agtgctgctg ctacgtttcg ccaacgattt
     6301 ggaggaatat gccaaagtag agcaattgcc gattttggaa ggaaaaagaa tgacaataca
     6361 gttatcgcca aaaaaatcaa aataaagaca ttaatttaat tcaattattc aatttactaa
     6421 atatttaagc gaaatgccaa agaaaaagac aaactctgga gcaaagaaaa gatttacgtt
     6481 gaccggaaca ggaaaaataa aaaggaaaca cgcttttaag agccatattt tgactaaaaa
     6541 gacaaaaaaa caaaaacgta acttaaccca cagcgctctc gttcacaaag ctaatttgga
     6601 gagtgtgaaa gagttattaa atttaaaata aagtaatttt tttattcagt aatcagaatg
     6661 cttagcccaa agtccgttga aaaaagacgg cgctgacatt cataaatttt atcgattatg
     6721 ccaagatctg ttaatcatgt tgcttcgcgc aaaagaagaa aaagaattct taacctgaca
     6781 aaaggttatt tcggcagccg caagaatgtg tggactgttg ccaaaaacac ttgggaaaaa
     6841 ggcttgctct atgcttatcg cgaccgcaag gccaaaaaaa gaagtttccg cgctttatgg
     6901 attcaacgta tcaacgctgc tgcacggctc gaaggttcat cttattcccg attgatggga
     6961 gcactacaca agtccggtat cgtaattaac cgcaaggtgc tcgccgattt ggccatgaat
     7021 catcccgaag cattcaaagc tatcgtacaa aaagcaaaaa atgcataaac cgttttccct
     7081 tgtaaaaaac ctttacgggg tttattggaa aattttaagt aaaaaaggct gactagaagt
     7141 aggacagcct tttttattgg atattgaaat gaaaatccgc aaaactcccg gtttcaatgt
     7201 ctcggtaaaa tttcaattaa tagctgataa acagtaaatt ttataaaaca ttaaatttat
     7261 acaattaact gatattcatt attttgtact gtaataaaag ccagttaagc cgaaatttca
     7321 ttcaccagcg gttttccatc cacaaaatcc tgaatgtttt gcagcgtgat acgggcgatt
     7381 tccgaaagag cttctttggt aaaaaatgct tgatgagatg taacgattac attgttgaaa
     7441 gtaagaagac gtgccagaat gtcgtcgtcg atcacaatat cggaatgatc ctcgtaaaaa
     7501 taatctcctt cttcttcgta cacatccaag ccggcataac ctattttttt acttttcagt
     7561 gcatctatca gagctttggt cttaattagc tttcctctac ccgtattgat aatcattacg
     7621 ccttctttca ttttatggat ggatttttca ttaataagat attccgtttc tttggtcaac
     7681 ggacagtgta aagaaataac atcggaaagt ccatacaatt catctagttg ggtatatcga
     7741 ataccgtttg tttcggcaaa ttgcgtatcg ggatacaaat cataggcaag aacgttcatt
     7801 cccatgcttt tcagaatttg aattacaatt tttcctattt ttcccgttcc gattattccg
     7861 gctgttttac cgttcatttc aaatcccatt aatccttcca atgcaaaatt tccttcacgc
     7921 gttcgagtgt aagctttatg aatttttcgg ttcagcgtaa gcatcaaggc aatggtatgc
     7981 tctgctatgg aatgagggga ataggccggc acacgcacaa cctttatttt gccttttgca
     8041 gcttttaaat caacattgtt gtagcctgcc gagcgaagtg cgatcagttt tacaccattt
     8101 tctactagaa tatcaattac ttccgcatca acttccgcat tgacaaaaat acaaaccaca
     8161 tcggctcctt tggtcaaaat ggcattgtct ttacttaaat gacttttttg aaatttaata
     8221 tcgaaaccat attgttgatt tttttcttta aaaaaaattt cgtcgtatga ttttgcgtca
     8281 aataagataa cttttgtttt catcataata taatttattt ttttaaagtc tgatggaacc
     8341 cacatgcctg aatatattca ttcgtgtttg tgtatgcaat acaggtgggt ttcatgttgt
     8401 tatttttatc cgatttattt gcttagttgc aatttaaaca tttgttgata agtttttgtt
     8461 tatctccgga taagtaattc gagaatgaaa gatacttttt attttttctt tcagaacctt
     8521 tttaacagtt tcgacatctc taaaactaat gggagcatct tttaattgtc cttcggcaat
     8581 ttgcgtattg ataatacttt ccaccatttg atctatgctt tcttctgtat attcgctcaa
     8641 agtacgggaa cgggcttcca ctgcatccgc catcatcaaa atggccgtct ccttagtatt
     8701 tgggagagga ccgggatatg aaaacacatc ttcagggggt tctatgccgg gattatcatt
     8761 gataaaggaa acataaaaat attttgtttt acttgtgccg tgatgtgtag taataaaatt
     8821 aataatttga ttgggaagat gatttttcct ggcaatttct actccattgg caacatgctc
     8881 aattatcagt tttgcagctt cctcgtactg catcgagagc aaaggatttt ttcctcctat
     8941 ttgattttcg gtaaaaaatt cggggttttt catttttcct atatcatggt aaagagctcc
     9001 tgttcttaca agtaaactat tggcattgat tttttttgcg gcttcagttg ccagattaga
     9061 tacctgaagt gaatgctgaa aagtaccggg agcaacttcg gcaaatttca tcaacaaatc
     9121 gctattgaca ttggttagtt ctaccaatgt tacagccgaa agtagtccaa acattttttc
     9181 gaatatatat atcagcaaat atgcaaacaa aaggaaaagg gaactgacag caaaactaat
     9241 aataggatac caatcgattt tggaaatatt tccttctgtc atgagttcga gcgaaagata
     9301 tacaacggta taacttaaaa aaatgtatag cgcagtttgt gccaattggg aacgctgtgt
     9361 catatccttt aaactggaga cggcaaccat gccagcagtt atttgcaaca aagcaaattg
     9421 gaatggattg tcggtcataa aagacacgat aaaaataacg ataatatgta cgaaaagagc
     9481 cgttcgagaa tcgtaaaata cacgaactat tatgggtaat aaagcaaaag gaacaatata
     9541 gtagcttaac gaagaatatc gaatagtcaa tgaagtaagc cctacaatta ggatgatgag
     9601 cagggacaaa aacagtaaat tattaaaatt gtccaagatt cttggtcgaa aaagataaat
     9661 gtaaagaatc aggagcgtaa ttaaaccgat taccaataaa attttgcctg ttaaaactat
     9721 agccgtttgc tcgaatgccg attttctgtt ttcgttttcg atttgtaaag atcggagtat
     9781 ggcataagct tcgggagaaa cgatttcacc tttatcgata attcgttcgc ctgattgcac
     9841 catgccaaca gtaagtgaaa gatttttaag caattcgttt ttggctgttt cggaagtgat
     9901 gctgtcatag cgtaggtttt ctaccagata attgttaata tctaattcct tgatattttc
     9961 ctgattgctt ttaactattt cttcatatgc agtttttggc gtgtaaattt catttaaggt
    10021 agcaagttgc gaaattcttc ccggtaaaat acggtagatt tttttcacat tccatttttg
    10081 caaattattg taatcgtcat tcgaaataat tcctttcgag taaatcgatt tcaacttttc
    10141 ttcaatgaag gtagtcaaat ccggggaaat attgccggac ctgctgctgt cgattttaaa
    10201 tttatttatc tgaatatgca ttacagtggt atccagattg aagtatggtg taaaattctt
    10261 gagaatttcc tttttctctt tttcaatttg ctcattgctt ttgtatatcg gaaaatcaaa
    10321 aggtgcagtc aaaagttcat acgaccaagg tttaccttct tcaaactgat aattgaaagt
    10381 gctttcatgc ggataaagtt ctacaattat tattaccaaa gcgaaagcaa gcaaaagttt
    10441 taaaacggtt tcaaatttct ttttgttgac tttcagtttc atgacttttt gttttttgat
    10501 ttactactgt aaaaatcttt atttctctaa tcagcaaaaa tttgtggaat gttcagattt
    10561 ttcttgttaa taattcctgt tttttcatca gaatataatt tattttttaa aggtctgatg
    10621 gaacctacat gtctaatatg tgtttgtgta tgcaacacat gtgggtttga tttaaaattt
    10681 tgttttgtaa agatttcgac gccgtaaaaa agctaaaaat taaaaaaaca cgccttatgc
    10741 ttatcagttt tatttacttg tgaatcaaaa attaagaata tgagccacta tagaaaaaag
    10801 agtgaaaata ccaaaagaat tgggttgata gcattatgtt gcatgtaaca tgtaaaatat
    10861 agttattcag atggtaaaga tacaacaaaa aattattttg aaggccgaat ttgaatcaac
    10921 gataaaatgt tttcatgcca ccaatttgat gcgatgttta atctgtgggt aagagatata
    10981 aaattttaca ttgttgtccg gtaaacattt ataaaaatta gtgaaaacca atatagaaaa
    11041 tgtcttacta taatatcgcc cctaatgggg cttagatttt gtgggttgaa tttttctacc
    11101 ataatttcgc tgcaatgcag ctttctctta agtcccatcg ggacgatatt atggtagtca
    11161 ataagttaca aatatcatta gagctccgta ggagcgacac tatttgctcc tttattttta
    11221 tcggacaaca ttaataattt taaaaattct tatgatgaat ggagcccgct ttaatttata
    11281 ttggaaagca gatgtttttc aagatgtgat tggtaatttc tatcagaaag gaacctcgcc
    11341 attattatta ttattatttt tcagcaaatt atccagtgca ggagagccag aagtattttc
    11401 ataaaaatta ccctcaggca cattcatttt ggaagaaata acctgatagt tttcgttgaa
    11461 aatatcatcg gagtcgatgt catcaagatt taaaaacttg gcaaattctt ttttgaattt
    11521 taaatggatg gtatcggtag caccgtttcg gtgttttgcg acaataattt cggctatgcc
    11581 aatcatggaa ttgccatctt tgtctgtagt caagttgtag tattcgggac gatgaataaa
    11641 gcaaactacg tcggcatcct gttctatggc acctgattca cgcaaatcgg acaattgcgg
    11701 acgcttgcca tcaaccccgc ttctgccttc cacacttcga ttcagctgcg aaagcgctat
    11761 aatgggaata tccaattctt tggcaaggct tttcaacgag cgggaaatgg tgcttacttc
    11821 ctgttcgcgg cttgaaaatc gcatttcact ggcattcatc aattgcagat aatcgataaa
    11881 aatacactgt actttgtgct ccttgaccaa ccttcgtgcc ttgctcctca attcggaaat
    11941 cgaaagactt ggcgtatcgt caataaaaac aggtgcatca atcaaatctt ttatgttttt
    12001 atcgagttta tcccattcat aagggctcaa atcgccattt tttattttta caccatcaat
    12061 ttcgcaaaca ttcattatca aacggtttgc taattgtaca ctggacattt cgagcgaaaa
    12121 aacgccaacc ggaacattgt agtcaactgc catattttta gccatagaca gaacaaaagc
    12181 agttttaccc attgcaggac gggcagcaat aataattaaa tcggactttt gccagccgga
    12241 agtaatttta tccaaactgt gaaaaccact tggtacacca cttgttccct gtttattggc
    12301 agcagcttgt atgcgctgaa tggcttcatc aatcaccggt ttgatctgaa taacctcctt
    12361 ttcgagatta cgctgcgcaa tttcaaacaa catttcttcc gactcttcta tcaaatcatc
    12421 cacatcggtt ttgtcgtcga aagctttggt ctgaatttca ctggaaatac gaataagttc
    12481 ccgagctaag aatttctgca ggataatatg cgcatgatat tccaaatgtg cagccgaaga
    12541 aacttttgca gtcaacaagg taatataata tgggcctcca atttcttcga gcgtaccatc
    12601 ccgacgcaac tgctccgtta cagtgtgcat gtcgataggt tcctgatgca aagccagcgt
    12661 cataatggct tcataaattt tctgatgggc gagtttgtaa aaacacgccg gttttaaaat
    12721 ttccgaaacc agaaaatagg catctttttc tatcataatc gctcccaaaa cagcctcttc
    12781 cagctcaagc gcctgaggtg gaagttttcc catttcattt ggagcaatca ctgtgggtgc
    12841 actattttgt tttttgttgt atgttttttt ttcggccatt ggtattttct atatgtttat
    12901 gtaaagtttg aattaacttt tcaaaagtac aattattttc caaatttgag agaatttttt
    12961 gtcaacagct gttgataagt tttataggaa aacaccaaat aatcctgaaa aatatgacct
    13021 taagctgtaa atatttttat cgttttatct gttttttcca taaaaagttc aaacaccaaa
    13081 gatttgacag gcctcacagt gtgagaattg aaaattatca tgtaactttg catgaaaatt
    13141 gaaattactt cgtacacatg aatatagaac ttagcgatca ggaacaatac agaagacaaa
    13201 gtctcacgga attacgaaat ttaggcatag agccttatcc tgctgcactt tatccaacca
    13261 atgctttttc tacagatata aaatcgaatt ttgacgaaaa caatccccag cgcatggaag
    13321 tttgcgtagc cggtagaatc atgagtcggc gcatcatggg aaaagcttcg tttgtagaac
    13381 tgcaggatgc aaaaggacgt attcagattt atattacacg ggatgatatc aataccgaag
    13441 cacaacccga aatgtacaac acagttttca aaaaactgtt ggatattggc gactttatcg
    13501 gtatcaaagg ttatgttttc cgtacccaaa caggtgaaat cagtgtccac gcaaaagaat
    13561 tgacggtact ttccaaatca cttcgtccac tgcctatagt aaaatacaag gatggtgtag
    13621 cctacgatgc ttttgacgat cccgaacagc gctacaggca gcgttatgta gatttagtag
    13681 tgaatgaagg cgtgaaagat atttttctta aacgaacaaa agtttaccaa tccatgcgcg
    13741 aatttttcaa tactcgcggt tacgtggaag tggaaactcc aattttgcaa cctatccccg
    13801 gtggcgctgc tgctcgacct ttcatcaccc accacaatgc tttggacatg ccgctgtatc
    13861 tgagaattgc ggatgaactt tatttgaaaa gattaattgt gggcggtttt gaaggtgttt
    13921 atgaattttc taaaaatttc agaaacgaag gtatggatcg tacccacaat ccggaattta
    13981 ctgctatgga aatttatgtg gcatataagg attatttttg gatgatggac tttacggaac
    14041 aaatgattga aaaaatttgt ctggatgtaa atggaacgac cgaagttcag gtgggtgata
    14101 aaacaataag cctgaaaacg ccttacaagc gtatttccat gattgatgcc attaaggaaa
    14161 atacaggtat cgatattgaa ggaatggatg aaaatgattt gagaaaagta tgtgaagatt
    14221 tgcaaataga tgtggacgaa acgatgggaa aaggaaagct tatcgacaaa ctttttggag
    14281 aaaaatgcga agccaattat gttcaaccaa cttttattac ggattatcct ctggaaatga
    14341 gcccgttgtg taaacgtcat cgcaataatc ccgaacttac ggaacgtttt gaattgttgg
    14401 tgaacggaaa agaactggcc aacgcctatt ccgaactcaa cgatccgatt gaccagttgg
    14461 aaagatttca ggaacagctt cgactaagtg agaaaggaga tgacgaagcc atgtttattg
    14521 acatggattt tgtccgcgca ttggaatatg gaatgcctcc tacttcagga atgggtattg
    14581 gtatggacag actggtgatg ctgcttaccg gtcagcaagc cattcaggaa gttttgcttt
    14641 ttccgctgat gaaacctgaa aaagttcaaa aagtcgatac caaagaaaaa ttcatggaat
    14701 tggatattcc tgaggcatgg gctgaagtac ttcaaaaaat gggaataaaa caggttgacc
    14761 aattgaaaga tttgaatccc aataaattgc atcaggacat ttgcggattc aacaaaaaaa
    14821 ataaacttgc attgaccaat ccttccaaag aagaagtcag ccaatggatt gataatgcag
    14881 cgaaaaaata atcattacga atcgttttac agtctgatat tgttatcttt agtttacttg
    14941 tattttagag tttaattctt tgtattgagt tggtttttaa attaaaaaaa attaatgttg
    15001 tccgataaaa ataaaggagc aaatagtgtc gctcctacgg agctctaatg atatttgtaa
    15061 cttattgact accataatat cgtcccgatg ggacttaaga gaaagctgca ttgcagcgaa
    15121 attatggtag aaaaattcaa cccacaaaat ctaagcccca ttaggggcga tattatagta
    15181 agacattttc tataatggtt ttcactaatt tttataaatg tttaccggac aacaatgaaa
    15241 aaaatctttt ttatgatgct caaatcttat gtttgaaatt gtttttgaac tttcttcttt
    15301 ggccgatact gcggtttttt atggaataaa caattccaat atgcagttga taaaaaattt
    15361 acatcccaat ttacggattg tggcgagagg aaatgttata aaaattattg gaaatcagca
    15421 ggatgcggac aagctgattg ttaacttgca gaagcttgaa aaattcagca tcgacaacaa
    15481 tcaacttacc gaacaaaata tcatcgaatt agtcaagggt gaaaatccga aggaaatttc
    15541 gtttcaaaac ctgattatgc acggtgttgg aggaaaatcc atttttgctc gcaccaaaaa
    15601 tcagcaacgt ctggtagaag aatttgaaaa aaatgatttg ctctttgcca tcgggcctgc
    15661 cggttcggga aaaacctata cggcaattgc tttggccgtt cgggcattga aaaacaaaca
    15721 aatcaaaaaa attattttga gtcggccggc tgtagaggcc ggcgaaaagt tgggttttct
    15781 tccgggcgat atgaaagaga aaatcgaccc ctatctgcaa ccgctttacg atgctttgca
    15841 ggatatgatt accccgctca agctgaaaga gtacatggaa aacggtacca tacaaattgc
    15901 accgcttgct ttcatgcgtg gacgaaccct gagcgatgcc gtcgtaattc tcgacgaagc
    15961 ccaaaattct accatcatgc agataaaaat gtttctcacc cgcatgggac tgaataccaa
    16021 aatgatagtt accggcgata ttacccagat tgatttgcct cgcgaccaga aatcgggttt
    16081 gattgatgca ctgaatttgc ttggcaacat caaaggcatt gcccgcatcg attttgatgt
    16141 caacgatatt gttcgacatc ggctggttca gcaaattgtt caggcttacg acaatcgggc
    16201 aatgatcgaa agacaaaaaa atactaaaga ataaataacc gaacgaatca tttgtgattt
    16261 tttccttttt ttctttaaaa ttatatcaac cctcaaaact aaaaattatg aatacccttg
    16321 ttcgcaccga ttttcatttc gaaggacaga ccaatgttta tcatggtaaa gttcgcgatg
    16381 tttactttat acaggatgac ctgctggtaa tggttgctac cgatcgtatt tcggctttcg
    16441 acgttatttt gcctaaagga attccgtata agggacaaat tttgaatcaa attgcagcca
    16501 agtttctcga cctcacagcc gacattgttc ttaattggaa actcgcctgc cctgatccaa
    16561 tggtaaccgt gggattaaaa tgtgaaggtt ttcccatcga aatgattgtg agaggttatt
    16621 tgtgcggcag cgcttggcgt gcttataaaa atggcgttcg agaaatttgc ggggttaaac
    16681 ttcctgacaa catgcgcgaa aatcagcgtt ttgaaacacc tattattacg cccactacaa
    16741 aagccgaatt gggaacgcac gacgaagata tttccagaca ggaaattctt gcaaaaggat
    16801 tgttgacttc tgaagaatac gatttgttgg aaaaatatgc actggcactt tttgaacgtg
    16861 gcacccaagt ggctgccgaa agaggattga ttttggtaga taccaaatac gaatttggaa
    16921 agagaaacgg aaacatttat ctgattgatg aaattcatac gccggattct tcccgatatt
    16981 tttatgcaga aggttatgag gaacgttttg aaaagggtga gccacaaaaa cagctatcga
    17041 aggaatttgt tcgtgaatgg ttgatggaaa acggttttca gggaaaagaa ggacagaaag
    17101 ttcctgaaat gactgatgaa attgtcaaca gtatcagtga aagatatatc gaactctatg
    17161 aacaaattac gggtgagtct ttccagaaaa ccgatacacg aaatcttgcc gaacgcatcg
    17221 aaagcaatgt aatgaaatat ctgcaaagct taaaaaaata aacctacgca tcggtaggcc
    17281 gataataaag aatccactct ttaattgaaa cctttcgggc tttaaagagt ggatttttta
    17341 ttggaaaatt ttcatttatc cttgtttttc accggttatc cgttcgagcc atttcaacat
    17401 ggccagcatg gtgaacatgg aaataaggca agccccgaca aaaacgctcc aagtcatcca
    17461 aatcggaatg tttttgtaca tcagtgcacc aagaaaaagc aactgatttc ccagtgccgt
    17521 agcagccagc caaccacctt gcatgattcc ctgatatttt tgtggtgcca cttttgaaac
    17581 aaaagaaatt cccagcggac tgataaacag ctcggcaacc gttaaaatga aatacgttcc
    17641 caacagcagt aatggcgtaa cgcgttcagc atcgggcaat cctcctctta aagtaatttc
    17701 ttctttcgaa ggcaagccca gcgaacccag cgtcatcaca acataagcca aagcagctat
    17761 tcccatacca atcgcaattt ttcgtggcgt tgaaggttct tttcccttgc tgcgcaagta
    17821 accaaacaaa gccataacca acggggtgag aaataccaca aaaaacgggt tgatggattg
    17881 aaaaatttct gctccctgca gcgtagtaaa tccaagattc acggaaactt tgctcaggtt
    17941 ggtataatct ttggcaaaaa aagtcagggt caagccgttt tggtggaaag agaaccaaaa
    18001 gaaaattacc actgcaaata cggccaacaa ggcatacaaa cgttgtttga tttctttggc
    18061 atccatctct ttaccgtttt ggtccaaagt tactgttgcg gttgaagata aattgcgagg
    18121 ttgaggaaag gattttttgt ttgttacaaa aattatcaac gaaacaaaca tggcgccaat
    18181 ggcaaccata aaagcataat gaaaaccggt ggtaaacaaa ttcagatact gatctgcaaa
    18241 agaagtcata tttgaacctg tgtaaccggc attaacagcc aattcatgca tccgttgagc
    18301 tgcttcgggc gtaatgatgc cattcaactg gtcatgacac aaagcaggaa gatcaggatt
    18361 ataagcaaag ccatgagagt gcaaccacca gtttctcacg ccaatagcag caaaaggagc
    18421 aaatatggct cccacattaa tgaacatgta aaacagtgaa aaacccgaat cacgcatttt
    18481 ttcgtacttc ggattatcat acatttgccc taccaatgcc tgcaaattcc ctttaaaaaa
    18541 tccattccct aaagctatgg tcaacaatcc gaaacaggtt aatccgagaa aaaacccgta
    18601 tctgttttgt ggaacaggag tgggtgtagg aagcgctact aagaagtaac caattgacat
    18661 taatattagc ccccacaaga tggttctttt ataatttctt gttttatcgg ctatcaatcc
    18721 tccaacaaat gccaaaatat aaattgaaaa ataaaaaaca gaataaatta accctgcttt
    18781 tgtttcatcc aatccgtatt ttgccatcaa aaacaacgac aaaatggcca tcatggtata
    18841 aaatccaaaa cgctccccca tattggcaag cgaagcagct ataagtcctt tgggatgatt
    18901 tttaaacata aacttgatct cattttagtg aaactatcag aagaataatt tataaaaatg
    18961 caaaagtgaa aaatttattt attccaatcc atatcctgac caaaaaaatt aaattgtttt
    19021 aactaattat ttcatctgac aacaattcaa taaaaattaa gaatggcttg acaaaaacga
    19081 cagaatttgt tgaaacattt cttcatccgt accactgaca accaacgctg ccttacgata
    19141 aaaaggttcc cgttccgaca agttttgtcg aataaaatcc aataattctt taccttctcg
    19201 ctgagccagc aagggacgtt tattcttgtg tgaaagttcg agccgttcct gtaattgttc
    19261 agggcttaaa cataaataaa cggttaagcc ataagcattc attcgttcca tgttatcgta
    19321 aaagcagggt gctccaccac cggtggcaac caccacattt tgttcggtgg atatttcttc
    19381 taaacattct ctctccaatc gccgaaattc ttcttctcct ttttcagaga aaatttgcga
    19441 aacggactgt tgcatttttt cttcaatcac atgatccatg tccaaaaact tataacccaa
    19501 tcgtgtagcc agttgctgac cgatagttgt ctttccggct cccatatagc caattaaaaa
    19561 tattcgattg tctttattag attgcatttt tataattttc ttttatcgct tttgtccgca
    19621 aagttaaatg tttaccctca acgaacctaa aattgtgcat ttattctgca attagcgcta
    19681 aaaaattgct aaatcaatta aataaaaaac cctgcaagaa aatatatctt cacagggtta
    19741 aatatttaaa atgaaaaaca attaatcatt cttccacaac tgcagcaggt tttatgggcg
    19801 tttttaattt aaaagtagta acgttgctcc cataaacatc atcaaactga gcgtcggaaa
    19861 ccaatgaaaa ttgaagtatt tcatcattgg ttgcgctttt agcagcaaaa tgaaacggaa
    19921 aatagacggc attgaaccca ctcagaaaaa caaattttcc ctgcccgtaa tcggaattgg
    19981 tgtgtgaaaa aacggagtcg aggcttgcta ccggaggaaa taaaaaatca ccggcactgt
    20041 caacggtttt tgtcaaacga aatgaaacca gattatttgt ataggcgttt aatccgagtt
    20101 gaaaaaagac agtatcacct acctgaatac tatccaaaag ataaacccca gattcattag
    20161 tagttttaat gtttaacgta tcaccatgtt gggtgtaggg gaaagctaaa aattgaatag
    20221 caggagtgta attggaattg ccatcaatat tgcacccggt taataaaaca accgccatga
    20281 acgatagaag aacaaactgt tttttcataa tattttgaaa tttgaagtga atgaaaggaa
    20341 gtaacaaaaa acataccaat caacctgaaa catcaaatcc aactttgtcg gggtgacaag
    20401 actcgaacct gcgacctcta cgtcccgaac gtagcgcgct accaactgcg ccacaccccg
    20461 ccttgattat tcggctgcaa atttacatca ttttttgata tttcgagcat gaaaataagc
    20521 taaaattatt cacagatgga tatccgcata attttaacca ttatttatgt gtttgtatat
    20581 tagttattta acaatttttt gaaactataa attaggagaa taagcgttga aattactacc
    20641 tttgtgaaaa ataattaaat tcatgtttac agaggctatt caaaattatc tgcaacgaca
    20701 cgactacaaa agtttttatc ccaaagccgt atttttcgat atggatggag tgctgatcga
    20761 ctccatgaag aaccattcca gagcatggat gcaagccatg aaagaatcaa acctgccttt
    20821 taccgaagaa gatgcctaca tgcacgaagg ttgtattggt gctaccacca tcaacgatac
    20881 ttttatgaag catctgaaca gggaagctac tgaagaagaa caaaaacaaa tttattcagc
    20941 aaaatcaaga atctttgaat cgcttgagcc catcgaaaaa atgccttttg cttatgaatt
    21001 gctcaaaaag ttgaaatcgg aagaaatgaa aatttatgtt gttaccggct cgggacaacc
    21061 caaattaatc gacagtctga atacgctttt ccccgaaatt ttcacaaaag aaaccatgat
    21121 aacgggtttc gacgtaaaag aaggcaaacc atcacccgaa ccctatttat tggcgctgaa
    21181 aagatccgga tttcaaccct gggaaacgat agttgttgaa aatgcaccaa tgggaatttt
    21241 atccgcttca aaagccggct tgtttacttt agttgtcaat acaggcccgt ttgacgatga
    21301 tttcctttat agctatggag ctgatttggt actcgacagc atggaacatt tgtacaatga
    21361 atgggacaat ttccgtaaat cgtggatttg aaatgaaaaa gacaggtttg gaacttttcc
    21421 tttctcccgt ttttctttcc ctgattttat tcttttcttc aaccgttatt tttgccggag
    21481 aaactgattc tgcttccttt catccccaaa tagaaggaac gatcagggcg aagtacgaat
    21541 acaacaccag tttgaacgct tcccgttttc aggtacgtaa cgctcgtttt agcgtcaacg
    21601 gaaattttaa ttccctcgtt tcctacaaaa cggaaattga tctttccgac gaaggtcaaa
    21661 ccaaaatgct ggatgcctat attcgactgc aacctcaaaa atggttcact ttcaccatcg
    21721 gacagcaaaa aattcctttc agcaccgata atcttcgtcc tcctcatttg ctgtattttg
    21781 ccaatcgatc gtttatcggg aaacaactca ccgcactcag agatgtgggc actacattaa
    21841 gttttgtaaa taataaaact ttaccttttg aatttataac cggtttgtat aatggcgagg
    21901 gtctttataa tcagaaaatt tggcaaactt cattcagtta tgcttttaga ttggttttac
    21961 atccttgcga aaatctacat ttttcattaa atcataattc tattcgtcct gaagaaatgc
    22021 ggatgaatct atatgatttg ggaggtattt tacagctcaa aaattggcat ctcgaaaccg
    22081 aattgttcta taaaacctat caacagaatt ttttccctac caccaaaggt ttttttgttt
    22141 tttcgagtta tgatatatat ctacataata aagaaagtat taaaattatt cgtccactta
    22201 tacgatacga cagtatgacg gacaataaca aaggttacaa atcggaaaac ggaagttatg
    22261 aatcagacga catggcaaga tcaagaatta caggcggaat aactttgtgt cttgacaaac
    22321 cttttttaaa tgatattcga ttaaactatg aacagtattt ttataaaaaa ggcattcaaa
    22381 acaatgacaa taaatttgtg gtagaatttg tacttcgttt ttaataaaaa agcattaaat
    22441 ttgcaatacg ttttttattt tctccaaaaa aacaaaaatc aggaagaaaa tcaattatgc
    22501 aagtaataaa atatccggcc agagaatttt ggtcaaccct tctctcccgc cccgccattg
    22561 atagaaaaag tttatttgaa aaagttggaa gcattgtttc agaggtgaag caaaacggag
    22621 ataaagcagt caaaaaatat accttacagt tcgacaaagt tggcattgaa gaacttgaag
    22681 tgagccaaga agaaattgaa gaagcatcct tgcagattcc aaaccagtta aaggaagcca
    22741 tagaaatgtg cagacgaaat atttggaaat ttcattctgc acaacaacac gatttaccgg
    22801 aaattcagac tacaccggga gtggtttgct ggcaaaaagc catgcccata gaaaaagtgg
    22861 ggttatatgt tcccggagga acggctcctt tgttttcgac cgttttaatg ttaggcgttc
    22921 ccgcacaaat tgccgaatgc aaggaaattg ttctttgtac acctcccgat aaagaaggca
    22981 aaattcatcc ggctgttctt tatgctgcaa aagttgcagg catacacaga attttcaaaa
    23041 ttggaggaat acaagcaatt gcagctatgg cttacggaac agaaactgta ccgaaagtgt
    23101 ataaaatttt tggaccggga aaccaatatg taactgctgc caaacaattg attgcgttgg
    23161 atggtattgc aattgacctt ccggcaggtc catcggaagt tatggtgata gcagacgaaa
    23221 gcgcacgacc ctgttttctg gctgccgatt tgctttcaca agccgaacac agtgtcgaca
    23281 cccaatcaat tctcattact gttagcgaaa atcttgtaga accggttctt ttggaaattg
    23341 aggaacagct caaccgcatt ccaaaaaaag aaatcgctct caaagctttg gaaaatggta
    23401 aaataattgt gctgcaatcg ttggaagaag cagtagcaat ggcgaacgaa tatgcacccg
    23461 aacatctgat tatctccact cgttacagtt tgggagttgc atccaatatc agcaatgctg
    23521 gctcggtatt tctcggacac tatacgcccg aaagtgccgg agattatgct tcgggtacca
    23581 atcacaccct gcccaccaac ggttatgcaa aagcctacag tggagttaat ttggacagtt
    23641 ttatgcggaa agttactttt cagcaaattt ctcgcgaagg actttccaat ttgagtaatg
    23701 ccattatttt gatggccgaa aatgaaggtc tttatgccca cagtaacgcc gttaaagtgc
    23761 gattggataa agaagaatca gaggaaaaac ccgaaaaccg gctttgttaa tacatgcatt
    23821 acacttttct tcgtttgaac aacaaattta gaatcaaaaa aatcacatga taaaattaga
    23881 ttagaatgaa aataaatgaa ttagtaagag agaacattcg taatatgcag ccatattcgt
    23941 gtgctcgcag cgaatttaag ggcgaagctt ccgtgtatct tgatgccaac gaaaatccgt
    24001 ataattcacc tttcaatcgc tatcctgacc cgctgcagac tgagctgaaa aaagaaattt
    24061 caaaaattaa gaaagttccc gttgaaaata ttttcttggg taatggcagc gatgaagcaa
    24121 ttgatctctt gtacagagct ttttgtgaac ccaaaattca caatgtggtt gccattgaac
    24181 ccagctatgg tatgtacagg gtttgtgctg aaataaataa tgttgaatac agaccggtct
    24241 tgcttgatga aaattttgat gtggaagctt ataaattaat ggatgccagc aatgacaaaa
    24301 caaaaattat atggctatgt tcacccaaca atcctaccgg caacagtttg aatttttcgg
    24361 aaatcaacaa acttctttca tggttcagag gaattgtggt aatcgacgag gcttatatag
    24421 atttttctac aaaaaaaagt ttactgaatt acctgactct tttcccgcaa ttgatagtat
    24481 tgcaaaccat gtccaaagcg tggggaaatg caggaatacg tttgggaatg gcttttgccg
    24541 atccggaaat aatcgggata ctgaataaaa tcaagtatcc ttacaacatc aattatctta
    24601 cccagcaaca tgctttacaa gcgttgcaaa atcctatgca ggtagaaaaa tgggtagcat
    24661 taattttgga ggaaaaagaa aaactgataa aagaacttca gaaattaaaa ttggtagaaa
    24721 aaatttatcc ttctgatgca aactttattt tggtaaaagt tgaaaatgcc aatgcaattt
    24781 atcgatttct cattcaaaac gggattgtcg tacgcaacag aagtaatatg cctttgtgta
    24841 acaactgcat acgcattact gtaggaaccc cagaagaaaa caaagcatta atcgatacac
    24901 tgaaaaaatt tcacgtgtaa aattttactc tccattacga aaaaagcgct tgaaaggaat
    24961 cctttcaggc gcttttttgt agcgggaacg agagttgaac tcgtgacctc cgggttatga
    25021 atccgacgct ctaaccaact gagctatccc gcctttttgt ttttgcggtg caaagatagc
    25081 tttttttaat aattccgaca accctttgca ataaaaatct attttttcaa tcttataatt
    25141 cggtagccgg ttcacccttt aacattacta aacaagctat tttaacaatg aaactcaagc
    25201 aaaaatattg ttcccgaacc atgatacagt tattccaagt tatacattct atttatctca
    25261 ttatcagcaa taaatagata agcatagaaa actattttgc aaatttcggg ttgaaataaa
    25321 aaaagaattg atattcaatc aatgcggttt tttcgtgggt attgatttat tgtaaccagc
    25381 agatgattaa atttcccatt gttcacaagc atcaatgagt tcatcaaacg attttacgga
    25441 agtttgtttt tttatttcat tgatttgatt ttccatagcc atttcataag tttcttcctc
    25501 cacatcacga ataattccca tagctatggg catttctgga ccttccatca atgccagttt
    25561 ggtatgtaaa aaagtatctt tgcacttggc atcatgaacc agaatatcct tatctgtaat
    25621 tccattttca ccaatcgtta cgacttttag ttctgatttt tccaaaatca atcctttatc
    25681 tttatttttt ccgaaaatca tgggcttacc atgttccaac acgacaattc tatcatctct
    25741 ggtttctttt tcggccaacc atccatgcgc accatcattg aaaatcacgc aatttaccaa
    25801 acattctact acagaagttc ctttatgaag ggcagcagcc ttcatgacca tttgagaagt
    25861 tttcaaatcg acatccaata atcttccaaa aaatgttcca ccggctccaa aagtcagttc
    25921 agccgtacga aaaggtcgct caactgtgcc ataaggagaa gatttggtaa caaaaccttt
    25981 tttggtagta ggagaatatt gtcctttggt aaggccgtaa atctgattat taaaaagtag
    26041 aacattaatg tccacatttc tccttacgct atgaataaaa tgatttcctc caatagccaa
    26101 gcaatcacca tcaccggtaa cttgccaaac cgtaagcgca ggattggcaa ctttaacacc
    26161 ggttgctaca caagcgcctc ttccgtgaat ggtatgaaat ccataagtat tggtgtaata
    26221 agggagacgg gaagaacaac caattcccga aataatgacc gtttcgtgtg gtgaaacacc
    26281 caactctgcc attgtcttga ccagagcatt gaaaatggca taatctccac aaccgggaca
    26341 ccagcgaggt tgctggtctg ttttaaagtc ggcaggcata tattttgtct cttccataat
    26401 ttctatgatt tttttggatg catgaaataa ttatagcagc gaaatgaaat gattttccaa
    26461 ttcttccgtt ttaaaaggct ggccttgtat tttgttgaat tgggtaaagg tttttccggg
    26521 aattttggat cgcagatacg aagcaaattg accggtattc agttcgcaaa caactatttt
    26581 ttcgtatttt ccaataacat tttcagtatt ggcgggcagc gggaaaagat aagtaaaatg
    26641 agccaacgct attttatatc ctttgttgtt caagttgtcc acagcacttt tcaaatgtcc
    26701 gtaagttcca ccccaaccaa ctaccaacaa atcggcatct gtatttcctt cgatttcgag
    26761 ttctggaatt acatttacga tattgtcaac cttctgtttt ctggttaaaa ccattttttc
    26821 atgattttcg ggatcggttg aaagatttcc ggttgtataa tctttttcca aacctccgtt
    26881 gcaatgttga aaacctttca ttcccggaat actccaataa cgaatcttgg tatcttcgtt
    26941 tcgagtggta accttgtagt tttccatatt cgatggaaca aaatgaggat gaatttctgg
    27001 taagtcggca agttttggca agcgccaaag agaacttcca tttccaataa aagcatccgt
    27061 cagcagaata acgggcgtca tattttccaa tgcaattttg ctggccataa aagcataatg
    27121 aaagcacgag tcgggagttg gagctgccaa aacagcaagc ggactttctc cactgcgtcc
    27181 atataatgcc tgcaacaaat caccctgttc ggttttagta ggcatacctg ttgatggacc
    27241 actgcgcatg acatctacaa ttaccaaagg cagttcagcc attacagcca aaccaattcc
    27301 ttctgttttc aacgacatgc ccggcccgga agtacttgtt gcagccaagc agccagcaaa
    27361 agaagcccct attgccattg taatgcctgc aatttcatct tcggcttgta cggttttaac
    27421 accaatacct ttttgcaaag ccaattcttg cattatgtct gtggccggtg taatgggata
    27481 acttcccaaa aaaagctgaa gtccggcttt ttcggctgca gcaatcaatc cccaagccgt
    27541 agctttgttg ccgttaatac ttgtgtattt gccctttggc atttcgtgcg atttttctat
    27601 cacaaaattg gatacggtca gatgaagatt ttcgccataa ttgtatccat ctgtaagagc
    27661 cttaatattt gcttgaagaa tttgcgtttt ttttctgaat ttattttcca gaaaatgaat
    27721 agctttatcc agcggtcggt tgaaaagcca gcaaaccagc cctaaagcaa gcatgttctt
    27781 gctgcgcaat acggttttgt tatcaaaacc aaagtcttcg agactgcttt tggtaagtgt
    27841 tgtcagtgga acaggaataa gctgaatggt gtctgaaaga gaaagctctt caaaaggatt
    27901 atcagtggaa aaattggctt tttttagatc agatggctga aaagaatcca aatcaaaaat
    27961 aacaatgctg ccttttttga gattttttgc gttcactttc agcgcagccg gattaagtgt
    28021 caccaagacg tctatttcgt ctcccggagt gtaaattttg ttggccccaa catgaacctg
    28081 aaatccggaa actccaccca gagtgccttg aggagctcta atttcggaag gaaaatcagt
    28141 aaaggtagat atttcgtttc ccaattctgc cgaaagttga gcaaacaaat ttcctgtcaa
    28201 ctgcatgcca tcacccgaat cgcctgaaaa tcgtataacg atattgtcca attgaatggt
    28261 ttgtgcttta ttcatttttg aaaatagtga atgaattaaa taatatctta caaaaacttt
    28321 tttaactatg tgtatttaaa aatcggcatt ttttggcgtg cgtggaaatg gaatcacatc
    28381 acgaatattg gacatgccgg ttacaaaaag aagaaatctt tcaaatccca atccaaatcc
    28441 cgaatgcggc gcattgccaa attgacgtgt ttcgagatac caccaaatat ctttatcggg
    28501 aacttccatt tcctttgctc tggctttgag tttttcataa tcgtcttccc gttgcgatcc
    28561 accaatgatt tcaccaattt tcgggaataa aacatccata gcgcgaacgg tttttccatc
    28621 gtcgttcagc ttcatgtaaa atgctttgat attttttgga taatcggtga gaataaccgg
    28681 ttttttgaaa tgtttttcaa cgagatatct ttcgtgttcc gattgtaaat caattcccca
    28741 acctaccgga aattcaaatt tttgtccttt tgccatggcg tcttccagaa tttttattcc
    28801 ttcagagtaa gttagccgaa caaaatcatt ttctatgaca aatttcagac ggtcaatcaa
    28861 ttcgggatca taacgattgt tgagaaattc caaatcatca ctacactttt ccaacaccca
    28921 acgcacacag tattttacaa agtcttcagc caattgcatg ttttcttcca gttcattgaa
    28981 tgccacttct ggttcgatca tccaaaattc ggacaaatgt cggggagtat tggaattttc
    29041 ggcgcggaaa gtggggccaa aggtataaat ggctcccaaa gccaaagccc ccaattcgcc
    29101 ttccaactga ccggaaactg tcaacccggt ttgtttgcca aaaaagtcat tgctgtaatc
    29161 gatacatccg ttttcagctt ttggcagatc ggaaagattt aatgtggtaa cctgaaacaa
    29221 ttgtcctgca ccttcacaat cagaacctgt aataataggt gtatgaaaat agaagaatcc
    29281 tctttcatgg aaaaaagtat gaatggccat agccatgtga tgccgaatcc ggaaaactgc
    29341 tccaaaagta ttggttcgtg ggcgcaaatg tgcaatttcg cgaagaaatt ccattgtgtg
    29401 ttcctttttc tgcaaaggat aggtttcagg atcggctgtt ccataaatta caatatcttc
    29461 agcctgtatc tcaacgcttt gtccttttcc cagcgattgg gccaatttcc cggttacacc
    29521 tatacaggca ccggtggtga tcggttttaa aaaatcgatt cccaatttat tgttgtctgc
    29581 tacaatttgg atattgtgta cggtagaacc gtcgtttaat tcaataaagc ttacattttt
    29641 atttcctctt cgggtgcgta cccatccttt aacgcaaata tcttcaccga agtcgatttt
    29701 ttttaaagca tcaacaattt ttgttctttt cataagttcg atatttttat tttcctctga
    29761 aattttttgt taacatcgga gaaatcaatt ctttgtccct tcttttaccc tttaactttt
    29821 ttacaaagat gcaaaattac aaatttcaaa cgaaaaacca gccttgtcga cataaaaaaa
    29881 gcgtctgaaa tactatgatc aggcgctttt taaattatat tattcaaaaa atcaacgttt
    29941 tacgggctct atatttccca ttttactcat ggcacaacgg aaacctatat cgttggaaca
    30001 ttgatcttga tcaagataac gacgagtgga aggatttaac cagtatattc ggtctttcca
    30061 cgaaccacct ttgtaaactc tagttttgtc ggaaatttga ggagccaaaa catctgtagg
    30121 atcaattttt ttcatattgg taatggctgc aatatcggaa gaatcagcat aaagctgaaa
    30181 atcggtcgaa atttttgaag taagatctcc atccttataa ttacgcacat catctccttc
    30241 cagcaccgta gtggcaattc tacccaaaga gtcgatttta aaaattttat tgcccacttc
    30301 atcttctttt tcaacaatag gcgtcaaata catatttcct ctaaaagaat tgtattcggc
    30361 aacatcttca aaactggtag cgcggtaaac atcaagaacc cattcgttta cattgccggc
    30421 catgttgtaa agtccaaaat cgttgggata atatgaattg acgggagccg tgatggtagc
    30481 tctatcgttc aggctacctg ccgttcccat catatcgcca cgtccacgaa caaaattagc
    30541 catcatttga cccaattgtt tgcgagatgg attacgtacc tggtgcccat tccatgggta
    30601 aatttttcct tcaggaataa atccatcttt gccggctttc aacccatagg cagcatattc
    30661 ccattcggct tcagttggaa gtcgaaaatc ggaaaccagt aaaataccgt ccgacatatc
    30721 cacttttctg atttcgccgg tcaggtcttt ttttggattt ttcccaaccg gaggttgata
    30781 gcttgcttgt ttaagatatt tttgcgtatt aaaaacaaaa ttgttaataa tgttatcaaa
    30841 attagtctca ttttttaatg aggcaaaatc gggtggtgct attatacctg ctttaaccaa
    30901 tgccaattcg tttacccgat cggtacgcca actgcaataa tccattgcct gttcccaagt
    30961 aacgcccaca atgggatatt gatcgaaagc aggatgtgta aaataatgtt caatgtaagg
    31021 ttcattgtaa gccaactctt cccgccatac tttgccattt ggataggttt tttccaccaa
    31081 ttttggagct gtttttccaa aaacgagatt catccagtga gtgtattctc gccaatcgac
    31141 atttctgatt tcgtattgat ccatgtagaa tgaactcacg gtaattcgcc ttcttcggct
    31201 gtttcgagga gcattcacca cttctccctt ttcgcctatt gtgaatgagc ctccttctat
    31261 ttctaccata cctggaggta cattggtttt aaaatctttt ctgacagcaa atccggctcc
    31321 cttttcatca ttgtatttcc aacccgttac gcgtgagtat ccttctttgt ttgattgacc
    31381 cgcacaacct aaaaacagaa gagctaaaca gaatgtcgaa actttaatta agacttttac
    31441 tctcatggtt ttttttgaat aattgttttt tgaactatgt ttaacagaat aaacgattta
    31501 taaatttata aattttgtcg taatattaat ttgttttgaa aacttctatg ttcttatctg
    31561 aatctataaa acaaattatt tgcaaatata gtacttttta aataatactt tcgttatgaa
    31621 gttccaaaaa aatatttttc ctgaagttat ttgtctcgat tcctttgtta ttaactgttt
    31681 ttttagaaaa aacgtataat tttcatttag ttttttcttt ttttaaagat aaatcaaaag
    31741 ttcgtcagct aaatttctta tttttgcaat ttgtagttga ttccgaatcc acaattttta
    31801 aaattcatgt ttttaaattt aaacggccgt ttggttgatg tttcttctcc tgttgtaatg
    31861 gggattgtga atgttactcc cgattcattc tatacgggta gtagattttt gagcgagaaa
    31921 tctatattaa cttcagttga taatatttta agtaacggag gtactatcgt agatgtagga
    31981 gcatactcaa ctcgcccatc tgccgagtcg gtttcggtag aagaagaatt gaaacggctg
    32041 tcgagagcat tagaaatcat attgaaacaa tttccaaatg ttattttatc ggttgatact
    32101 tttcgatcaa aagtggccag atatgtaatt gaaaattttc atgtagccat gatcaacgat
    32161 ataggcggtg gtacacttga cgaattgatg tttgaaacag tagccgactt gagagttgct
    32221 tatgttttaa tgcacatgcg gggtactccg caaacaatgc aaacattaac gcattatgaa
    32281 aataatgttg tttctgaagt tttttcgttt ttacagaaag gtctggctca gcttcatcta
    32341 ttgggtgtga aagatgtgat agttgatccg ggttttggtt ttgccaaaac tttggaacaa
    32401 aattatgaat tgttgcataa actgcattat ttaaaagagt tgaacgcccc gatattagtt
    32461 ggtttgtcaa gaaaatcaat gatttacaat ttacttcgac tttcacccga agaggcttta
    32521 accggaacaa ttgcagccaa tatgctatct ttgataggcg gagcctctat tttgagagtt
    32581 catgatgtta aagaagcttt acaaactata cggatatttg agcagtatca aaacttttcg
    32641 acttttataa atgattaaaa aaagaattta tgttttttca gattgaattt aaagatattg
    32701 tcgatatttt tcttgttgct tttttgcttt atgaaattta caaattattg agaaattcag
    32761 gggctataaa tatttttatc ggcatattga catttattgt ttgttggttt tttgtgtcgt
    32821 atgtttttaa aatgcagttg cttggtgaaa ttatggatac ggtagtaaat gttggagcat
    32881 ttgctttgat tgtccttttt caagatgaaa tccgacgttt ttttactcaa attggcacta
    32941 aaagagaaaa cagattttta tcgaaaatga aaaaaatttt tggagaatca gaatacgata
    33001 aaaaggaaac agattttgac ttgttgcagg ttgtattggc ttgtcgcaat atgtcgagaa
    33061 caaataccgg atgtttgatt gttattacaa ggaggaatgc tttggaattt tatgttcagt
    33121 cgggtgaatt gattgaagca aaaatcaatt caagattaat agaaagcatt ttttttaaaa
    33181 atagtccgct tcacgatggg gcgttgataa tttcccgaca caccataaaa gctgccggct
    33241 gcattcttcc ggtttctcaa aaccaatcga taccaaaaag gttagggttg agacatagag
    33301 cggcgctggg cattaccgaa catactgatg ccgtagctat tgtagtttcc gaagaaacag
    33361 gtcatatatc ttgggcggtc aatggaaatt tgactcacaa cgttaaaccc gaagagttgg
    33421 aatatttttt aaatacccaa cttcagggaa gcaacaaagt ggaggaagaa aaaataaaat
    33481 aaaccgatac tttttcctta gattttttta gaaaaagtat cggtaaatta tttaatcaaa
    33541 tatcatgttt tcaggccttt cctgcacttc ctagtacatt caccgttttg tgcatgtaca
    33601 attttttcaa ttcatcgcgt gcaggtccca gatattttct tgggtcaaat tctgccggct
    33661 tggtagcaaa aacttcacga acagctgctg tcatggccag acgaccatca gaatcaatat
    33721 tgattttgca cactgcagaa gtagctgctc tacgcaactg atcttcggga acaccaatgg
    33781 cgtctttcaa tgcgccacca tatttgttga ttttttccac atattcgtga ggtacggagg
    33841 atgcgccgtg taaaacgatg gggaaacccg gaattcgttt ttctatctct tccaatatat
    33901 caaagcgcaa tggaggtgga atcaaaattc cgttttcatc gcgtgtacac tgttcgggtt
    33961 taaatttggt agccccatgt gaggtcccta tggaaatagc cagtgaatct acattggtgc
    34021 gggttacaaa atcaactacc tgatccgggt cggtgtaggt atggtgctcg gcaacaacat
    34081 catcttcaat accggctaaa acacccaatt ctccttcaac agttacatca aactgatggg
    34141 catattccac aactttttta gttaaagcaa tattttcttc gtaagggaga tgagaaccat
    34201 ctatcattac cgaagaaaaa cctgattcaa tacagctttt gcagagttca aaagtatctc
    34261 cgtgatccaa atgcagcaca atgggaatat tgtaacccag ctctttggca tattcaacag
    34321 ctccttgtgc catatagcgc aataatgttt ggttggcata tttgcgtgca ccgctcgaca
    34381 cctgcaaaat taccggcgat tttgtctcta cacaagcaga aatgatagcc tgcagctgtt
    34441 ccatgttgtt gaaattaaat gccgggatag cgtattttcc ttcaattgct tttttaaata
    34501 actctcttga attgactaat ccaagttcag tgtaatgtac catatttttt gaattttaaa
    34561 taattaaata atcaaacttt gcaaaattac gaatttttcc tgacaaatga aaagaaaaat
    34621 cttcactttg ttttttcatt ccaatcaatt ttctatcaac taaaacagaa ttttcttgcc
    34681 aattgtttga aatagtaaaa taatttttct taaaaatttt attaatcaac ctcttaattt
    34741 aattttgcac attatcaaac attttaaatt taaagattat gaaactttgg aaagagttta
    34801 aagaatttgc catgcgtggc agcgtaatag atttggcaat tggtgtaatt attggaagtg
    34861 cttttggaaa aattgtttct tctcttgttg ctgatgttat catgccacca attgggttgc
    34921 tcatcagtgg tattaatttt acggatttga aattggtttt aaaaccggct gagatggtaa
    34981 acgatgttca aactcctgcc gtaacattaa attatgggaa ttttctgcaa gttgcttttg
    35041 atttcctgat tgtggctttt gtgatttttc ttttgataaa aggtatcaac aatttcttga
    35101 aaaagaaaga agaaaaacct gtccctgaag caacagcaca accgagtaaa gaagaaattt
    35161 tgctgaccga aattcgagat attttaaaag agaaataaaa aacaaacaaa agagatgctt
    35221 ggtttatgtt gcataacaaa cctcagaaca gagacaaaaa cacaatttca attccttttg
    35281 gtgcgattaa aagtttctcc gcagttgtgg agttccgcat aaggaagtaa ggactgcaac
    35341 cagatggaat agatgtcaat cttttcggaa cgcaatgatt tgcgcaatgc gcctgtccat
    35401 atcatgagca aaccaatgga attgtgtttg ttcgggttcc atgcttcggc aaagaaggtg
    35461 ttgcgttcga aattacgatc gaattcgtca ttcattttct cctgaatatc tttgagtaat
    35521 aattgaaaaa gttggttaat tgtttgcatt ttaaaaattt atattgtatg tttgctttcg
    35581 gttgagggga acatcacccc ccgaccccga ccttgggatt agctgtaaaa gttaatccca
    35641 ttgtattttt aacataccgg acaatatttc ctccctgaga tattgtttat caaaaaatat
    35701 cccggaagat gacaaaaaat tgtgcaaaca tttatattat ttattttcag atatttaatg
    35761 atgaaaattt gccacaaacc ccacacaaga aaaatgggcg ttcgttggct aaaatctttt
    35821 aaactcgctc cttcgtcgct caaacagaaa agattttttt aacgccaact tctttttagc
    35881 tacgctgacc gttggttcgg ctcaccggct caggtcgctt gtttgcttca aacaccgttt
    35941 tgtcggattt gcaatatgac aggatattgc cggtggaggc ataaaatcag cggaaaaaat
    36001 cagcgtaaat cagcatgaga aaaacctctc tcgccgatga ttgctgataa atatcgcaga
    36061 taaacgcaga aaatcaataa aaatcaccgg aagtctgcgt gagaaaataa atgtgggttt
    36121 attcaaacct tatttaccac cactttaata acacaacaaa tccaccaccc aaattgaatt
    36181 ttatgagctt attaaaaatt aaaaaatccg gagattttat ttttacctgc tacgcaggca
    36241 ggaagatatg acaaatagaa aatgttcaac taaaaaatgt ttgtcctttt cttcgtctgt
    36301 tttaaaaatt taacgttata tttgttatct ttttagcaat acctcaattt cataaaaaca
    36361 actgcaacat taaataaaag caataaacac aaaaatttta tgactcatca aaacggactt
    36421 gacgtaaaaa cgctcgaaaa ctggttgtgg gaagccgcct gcaaaattcg cggacccatt
    36481 gatgccccca aatacaaaga ttacatttta ccgctcattt tcctcaaacg actttccgac
    36541 gtattcgaag acgaaatcaa tcgtctttcg caaaattacg gttccagaga aatagtggag
    36601 caatttctat ccaacgacca taatcttgtg cgtttttatc tgccgcccga aacccgttgg
    36661 gaagccatag ctaaacatac tacaggcgtg ggcgagtatt tgaccaatgc ggtgagaaaa
    36721 attgccagag aaaatcccaa actgcaaagc gtcatcgaca ttgtggattt taatgctaca
    36781 gccggcggac aaagaatcat cagcgacgaa gttttgaaag accttatcaa cgtgcttgga
    36841 aaatatcgat tgggattgga cgatgtggaa cccgacattt tgggacgggc ttacgaatat
    36901 ttgctcagaa aatttgccga aggttcggga caaagtgccg gcgagtttta tacacccagc
    36961 gaagtagccg ttttgatgtc gtatattctc gatccgaaac ccggtaacga aatttacgat
    37021 ccctgttgcg gttcaggcgg cttgctcatc aaagcttttt tgcgaagcaa ggaaaaattc
    37081 gacgataatc cctccttcga accggtacga ttttacgggc aggaaattct gcatgctacg
    37141 tatgcgatgg caaaaatgaa tgcttttatt cacgatatgg aagccgaaat agcactgggc
    37201 gacaccatga accgtccggc ttttttaacg tcggcaggtg ccctgcggaa attcgacctg
    37261 gttacggcca atcccatgtg gaaccaggat ttttctcaaa cggtttatga aaatgatccc
    37321 tacaaccgtt tcgtctttgg ttttccgcct tccaacagtg ccgactgggg ctggattcag
    37381 cacatgtttg cttcgctgaa cgatgccggg aaaatggctg tggtgctcga tacgggcgct
    37441 gtttcaagag gaagtggcaa tgtcggcagc aatcgcgaac gcgatatccg caaggaattt
    37501 gtcgaaagag atctggtgga agcagtcatc ctgttgcctg aaaatctttt ctacaacact
    37561 acagcaccgg gaattattct ggtaatcaac aaagccaagc cagccgaccg aaaggaacat
    37621 ttgttgctca tcaatgcggc tcatctgttc gaaaaaggcc gaccgaaaaa ctatctgccc
    37681 gacgaaagca tcagccgcat tgccgatatt taccttcaat ggaaagaaga ggaaggcatc
    37741 agcaaaatta tcaccaacca agaagcagcc cgaaacgact acaacctgag ccccagccga
    37801 tacgtggcac aaaacggcga ggacgatacc ctgccacccg aagatgcggt gattcagttg
    37861 cgagaagcgg aagaagaacg acaaaaagca gatgaaaaac tgaaagcaat tttacaagaa
    37921 atgggattat ggaaaaatta aaaaatcaaa tgaaaattag ttcttctccg ggaaagaaat
    37981 agataattca atatattgat tataagggtt catagaggag aaaataaaga taattgaaag
    38041 aaagaatgac atacaatcca aataaccatc atcgccgttc catacgattg catggatacg
    38101 attattcgca accaggtatt tattttatta ccctttgcac atataatcgt gaatgtttat
    38161 ttggggaaat tttaaatggt gaaatgcgat taaatgaatt tggaaaaatt gcaaaccaat
    38221 gttggttgga aatacccaat cattttccac aaacccaatt ggatgaatat atcattatgc
    38281 cagatcatat tcatgggata atcgtattga atgatttcgt aggggttcaa aatgttggga
    38341 cgaaaaatgt tgggacgaaa attgtagggg cgaaaaattt ttcgccccta ccaatgccaa
    38401 cgcccgaacc aacgcgcgaa caaaatatca cgccatttcg ttcgccatca aaaaccattg
    38461 gttccattat tcgtggattt aaaattggcg ttaccaaatg gttccgtcaa aatacggata
    38521 tttacgttgt ttggcaacga aatttttacg aacacattat tcgaaatgag gttgaattaa
    38581 accgaattag gcaatatata atcgataatc ctaaaaaatg gaaaacagat gaaaattaga
    38641 tttgataggg cgaaaaagaa aaagtctccg gcaacgttta gataatgtta gggcgaaaaa
    38701 tttttcgccc cgactataaa aattaaacaa tgattctatg attatgaact caataaacga
    38761 cgaaatatta ctgccacagg gctacaaaat gaccgaactc ggaccactgc cagaagaatg
    38821 ggaagtggta aaactgggag aagtttgtaa agtggattgg ggaaatacct cgttaactaa
    38881 aaaattatat acagcaaatg gttatccagc attcagtgcc gcaggaaatg atggtttttt
    38941 agatttctat gaacacgacg gtgaagctgt gatattatca gctattggag caagatctgg
    39001 aaaatgtttt tatgcttctg gtaaatggac agccattaaa aatactattg taataaagag
    39061 taataatgaa acaaaaatta atctgttatt tttatatttt tttgtaaaca atgaaaatta
    39121 ctggcataaa tccggcagtg gacaaccatt cataagcata ggtgtagctc aaaaacaaaa
    39181 actccctctc cctccacttc ccgagcagca gaagatggca gcggtgcttt ctgcggtgca
    39241 ggaggcaaag gagaagaccc atgcggtgat agacgccaca aaagccctta aaaaatcgat
    39301 gatgaaacac ctgttcacct acggacccgt gtcgccggaa gaagccgcaa acgttccgct
    39361 caaggaaaca gaaatcggga aagtgccgga agattgggag gtggtgagac taggagatat
    39421 tgccgaaaca tacagtggag gaactccaag acgagaccat aaagaatatt ttgagggtaa
    39481 aattggatgg ttaaaatcgg gagaattaaa cgataattat atttattcaa ctcaagaact
    39541 tatatcagaa gaaggattaa gaaaatcttc tgctcaatat gttgaaagca atacgctttt
    39601 gattgcattg tacggtgcta ctgcaggaaa agtcggatta gcaaaaagaa aatttacaat
    39661 taatcaagca atatgtgcag taaggcctaa taataaattc tggacagaat tttattttta
    39721 ttattttcaa acaattagag ataaactttt atcaatgaga tttggatgtg ctcaaccaaa
    39781 tttgaatcag cagatagtta agaatactat tctccctctt ccccctcttc atgttcagca
    39841 aaaaattgct tcgattcttt ctgccattga tgaaaaaatt caggcagaag aggcaaagga
    39901 aaaagcactg gaagatttgt ttcgctcgtt gctgagccat ttgatgagcg ggaaaataag
    39961 ggtgaaggat ctggggattg gtaattgttg attagtgatt ggtgattggt gattggtgat
    40021 tagtgattgg tgattttttt gtaaaacaac cattataaaa tacacaggat atggcaaaat
    40081 taggcggaga acgatattcg gttcaacaac ccattattca gtatgtgcag gaaccatcgg
    40141 cagcatacgt ttcgcaggat ggactgacca tgtttctcaa actcggctgg gaatatgtaa
    40201 aacccgacga cgcactgcgc ctgaaaggcg gcgaagccgg catggtattt cgtgaaattt
    40261 ttatccaaca gctccagcgt ctcaatcccg actttctgga tcacctgatg gcaaaagaac
    40321 tgattaaaaa aatcgaaaca gtgcctgcca atatcgaggg aaattttctg gcctgggaat
    40381 atctgaaagg actcagaact gtttttgtcc ccaccgaaaa acgggaacgc aacgtaactt
    40441 ttatcgacgc cgaaaacatg gatcgcaata cttttcatgt aacggacga
//
LOCUS       DAOTAX010000012        40068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_810_length_40068_cov_31.586834, whole genome shotgun sequence.
ACCESSION   DAOTAX010000012 DAOTAX010000000
VERSION     DAOTAX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 40068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40068
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_810_length_40068_cov_31.586834"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLM04_02565"
     CDS             complement(<1..508)
                     /locus_tag="PLM04_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HON01993.1"
                     /translation="MGKTVTIYCKNTRSYHDFPLGTSLIEIYRSLNIQLKYQVVAARV
                     NYKVEDLNFLIYKPKDIEFIDLSTSSGMRVYVRTLTMVVAKAVSELFPKGNIRIEHPL
                     SKGYYCVLNNIDEPLTQSLVDKIKERINLIISQGKTIICEEKQTSEVIKLFADQKDKI
                     VLFQTLGNP"
     gene            641..2440
                     /locus_tag="PLM04_02570"
     CDS             641..2440
                     /locus_tag="PLM04_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HON01994.1"
                     /translation="MHYTFYDLLTLIGSLGLFLYGMKTMSEGLQKATGDTLRSIMAAM
                     TRNRFTGILTGLLITSLIQSSSATTVMVVSFVNAGLLSLYQAITVIMGANIGTTITAW
                     MISIFGFKVSIAAFSIPLIGISIPLIFSSKSRNKSIGEFLIGFALLFMGLELLKNSVP
                     DIQGNPEVLNFLAKYTNFGFGSILLFLLIGTMLTIIVQSSSATMAITLIICSKGWISY
                     ELGAAMVLGENIGTTITANLAAIPTNVSAKRAALSHLIFNIFGVIWMLIAFRPFINLV
                     TMLITQFGPGDPTQLTAFSNSLDPDTLKVISSGSENLSPEQIAVRNQLENYQIATSYG
                     LSLFHTLFNITNTLVLIWFAKIIEKISTLLIKKKDTDEEFQLQFISTGLLSTSELSLI
                     QAWKEIKVYAERSHRMFSLVRELLQEKNENDFIKKFSRVQKYENISDRMEVEIAHYLT
                     KVAEGKLSEKGKFQIQTMLKVISEIESIGDSCYNLARTIKRKKDDKAEYTKEMDDNLN
                     EMMNLVEDALLQMRAAIDNNHVSKEELNRAENLENEINNFRNQLKNKNIDDVKENKYD
                     YQASVTYMDMLEECEKLGDYIVNIVEALYEASK"
     gene            2662..3597
                     /locus_tag="PLM04_02575"
     CDS             2662..3597
                     /locus_tag="PLM04_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HON01995.1"
                     /translation="MDYILIISGFAVLVLGANLLVDGASSLAKKLKISDLIIGLTVVA
                     FGTSAPELVVNVIASVKPNATDIAISNVIGSNIVNIFIILGFSALFHPIKSEKTTTLF
                     DIPLSFFAIVVTYLLAFTGDRILSRTDGLVMLGFFIAFIYFTTRRSIKHPLIPLEENI
                     KIRKTGISILMISCGLAALIGGAEIIVPSAIRIAENLGISQAVIGLTIVALGTSLPEL
                     ATSVVAAIKKNSDIALGNIIGSNIFNIFLILGVSSVIRPLPIYRGIGIDLTMGALGSV
                     MVFVFVFGSKSHSIKKWQGLLLIFIYLMYAIFKIF"
     gene            3638..4762
                     /locus_tag="PLM04_02580"
     CDS             3638..4762
                     /locus_tag="PLM04_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HON01996.1"
                     /translation="MNLFDVYPLFDIEIVKGQGCRIYDKNDTEYLDFYGGHAVISIGH
                     SHPYFVKKLADQIQKLVFYSNSVKNSLQVELAEKLGKIAGYDDYSLFLINSGAEANEN
                     ALKLASFHNGRSKVIAFGKSFHGRTSAAVGVTDNPAIQAPINRNFEVEFLPLNDIEAV
                     LQSLKNRDVCAVIIEGIQGVGGIQIPQPAFLEAISAACKETGTVLILDEIQSGYGRSG
                     KFFAHQYADIRPDIITTAKGMGNGVPIGGVIISPMFKPTYGMLGTTFGGNHLACTAAI
                     AVLDVIEQEHLMENALRVGNYLINELQKFSRLKEVRGKGLMIGIEFDEPVKKLRERLL
                     FEKKIFTGVSGSNIIRLLPPLCLTMQDAELFLDHFAEVIK"
     gene            4785..5555
                     /locus_tag="PLM04_02585"
     CDS             4785..5555
                     /locus_tag="PLM04_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON01997.1"
                     /translation="MKKPIALLFILFFVNSWTVFSQSVSKERDKVVMKTGEIYYGEVV
                     FRNDEMLILQTDEGVRYQFSIAEVKAIYKETVDGSDELEKMQQEGQSDEIQNFFAVLD
                     ACGGISSAKNSFDVSSNTQVAFVLGKDKVFSENFFVGLGVGYNSSYLKSNSSLNFLPV
                     FLRLQNTFTNNRISPFVRMDAGYAFSMTSGWDGGALVKAAAGISSRLSYKTKLFCGIY
                     AGIQQFGGKLSETISSGTYYYEGTTNMRQLGVQLGIQF"
     gene            5602..6315
                     /locus_tag="PLM04_02590"
     CDS             5602..6315
                     /locus_tag="PLM04_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HON01998.1"
                     /translation="MNKINLSQVIIAALNEQACAEKAQHHLRFFKTAPGEYGEGDKFI
                     GVNVPDQRKIAKKYFREINLNELEVLLTNEIHECRSTALFMLCYKFDKAGEAERTLMA
                     DLYLNNLSFVNNWDLVDASAANILGCYLMDKKRDLLYDFVQSNDLWKQRIAIIATFYF
                     IRYGEFEDTFKISEMLLHHPHDLIHKAVGWMLREVGNRDKEAEINFLLKHYRQMPRTM
                     LRYAIEKFDEGLRRQFLKG"
     gene            6545..9004
                     /locus_tag="PLM04_02595"
     CDS             6545..9004
                     /locus_tag="PLM04_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HON01999.1"
                     /translation="MKLKEIAQILGNSHLKVPEVEINWLLTDSRALSFPTRSLFFALR
                     TRRNDGHRYIQELYDQHLRYFVVSETLPEFEKMHDAIFIRVNDTLEALQHLAAVHRNA
                     FQIPIVGITGSNGKTIVKEWLYQLLHSDFNITRSPRSYNSQIGVPLSVWGLNNNTELG
                     IFEAGISQPHEMERLEPIIRPTIGIFTNLGDAHQENFSSLKQKCEEKLKLFAHAQVLI
                     YCSDNRLVDITVAQSNFKGKLFSWGRNENAVLQLVKTKKLSDSTEVVYKFSGKESAVS
                     IPFSDDASIENAMHCVALMLHLGIDLEKIRQRIAKLESVEMRLELKEGIHGCLLINDS
                     YNSDLNSLAIALDFLNQQAATKNLPRTLILSDILESGKQSDDLYNAVAQLVKSKSILR
                     FVGIGPEIVKHADKFEVEQQNFFEFTEDFLKSPLIHELQKEIILLKGSRKFHFEKISE
                     KLELIAYETTLEVNLNALINNFNYFRSKLRPETKVMSMVKAFAYGSGSVEVARTLQLH
                     HCDYLAVAVADEGAELRREGIRIPIVVMNPEKGSFGMIFEHQLEPEIYSFRILESFLS
                     AAEKLGVTDYPVHIKIDSGMHRLGFEPHEIVPLIQRLKAQNNLKVRSVFSHLAGADEK
                     RFDDFTRQQVKVFTECADLFAQSFEHPIIRHILNSAGIERFPEFQFDMVRLGIGHYGV
                     SALPKVKLEPVCALKSIILQIKNVKKGETVGYSRKGIANEDKRIAVIPVGYADGFDRK
                     LGNGVGEVLINGKRAKVMGNVCMDLTMVDITGIEAKEGDSVEIFGNNLSVIEVADKLK
                     TIPYEVLTSVSKRVKRVYFQE"
     gene            9062..9832
                     /locus_tag="PLM04_02600"
     CDS             9062..9832
                     /locus_tag="PLM04_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HON02000.1"
                     /translation="MILIFVQNTTPPVALLQSVAILSVQLKKEWGILCLTENADLVSV
                     YENQYGEFLKTNGFPEVSIFVRQACCEQLVEFCEQLEASFLFFQLHDASHRYLRRSLN
                     FCRDLRIPYLFFKNSFSVLNLEKVVVPIGFLEEEIEKAQFASAFGRFSNAEIILLPAN
                     DYGSKAATTTDKMKTILDKFQLPYTVEKAQSDSFKIDREAIEKAVEMGGGLVIISASR
                     DYGLDDILFGPKELHLIRKSPVPLLLVNPRSDLYALCD"
     gene            complement(9849..10499)
                     /locus_tag="PLM04_02605"
     CDS             complement(9849..10499)
                     /locus_tag="PLM04_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HON02001.1"
                     /translation="MKSLHIGYIPDLDKADITTASEILEKNGTKAAIESQNWAKEFPY
                     KPIAYFHIAQSKEAVFIKFFVLCNALRAVYTHDQDPVYEDSCVEFFCKTIESEKYINF
                     EFNCIGTCSASRRKGRNDEVEPLSETEMKSIERFPSIVPQPFEEIKGNFDWELTVKIP
                     FKLIGIDPNNLPEKILGNFYKCADGTESPHYLSWNPIKTEKPDFHRPEFFGELFLK"
     gene            10931..12250
                     /locus_tag="PLM04_02610"
     CDS             10931..12250
                     /locus_tag="PLM04_02610"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HON02002.1"
                     /translation="MKVLKFGGTSVGSAQRMKNVAKLICNGEKKIVVLSAMSGTTNSL
                     LEIADYFYKSNLDGALEHIITLENKYSDVVEELLTTEEKRQEARSILSSHFDYLRSFA
                     KSVFTIFEEKAIVAQGELLSTALFQLYLEEIGENSILLPALEFMRIDKNAEPDNAYIK
                     QHLNDLLEAHPGHEIYLTQGFICRNAYGEIDNLQRGGSDYTASLIGAAVNAEEIQIWT
                     DIDGMHNNDPRFVESTKPIPMLHFEEAAELAYFGAKILHPTCILPAKLANIPVRLLNT
                     MEPEAAGTLISTRMNKGKIEAVAAKDGITAIKIQSGRMLLAYGFLRKVFEIFESYKTP
                     IDMITTSEVGVSVTIDNTKKLDEIVNDLKKLGTVTVDHDMVIVCVVGDLPFENVGFQA
                     KVVDALKDIPLRMISYGGSNYNISFLIKAEDKKRALNALSQALFSEE"
     gene            12300..13463
                     /gene="lysA"
                     /locus_tag="PLM04_02615"
     CDS             12300..13463
                     /gene="lysA"
                     /locus_tag="PLM04_02615"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HON02003.1"
                     /translation="MKAKYPVEKFLKLQTPFYYYDMDILRQTLQEVNRQIQKRNYVVH
                     YAMKANANPVLLHEIKNAGLGADCVSGGEINAAIKAGFPKEKIVFAGVGKADWEINLA
                     LDENIFCFNVESIPEMEVINQLAAAKSKTASIALRINPNVNAHTHQYITTGLNENKFG
                     INLSDLDTVLDLLPQLKNLKLIGIHAHIGSQITDMTSFQSLCVRINEIQNYFFQRHII
                     LEHINVGGGLGINYEHPNHFPIPDFESYFNVFAEHLNLQPYQTLHFELGRAIVAHCGS
                     LISRVLYVKQGATKKFVILDAGFTDLIRPALYQAYHRIENLTSDLPEEEYDVVGPICE
                     SSDTFAKNYSLNGTRRGDLVALRSAGAYGEIMASQYNLRSLPKAYTSDEIAAL"
     gene            13569..14732
                     /locus_tag="PLM04_02620"
     CDS             13569..14732
                     /locus_tag="PLM04_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02004.1"
                     /translation="MTEIFGFSLSLLTLVLLCGLFLVFCYQIYFYCRYINGVIRYRNK
                     IRKGKVAFLDEKPGVSIIICSKNEANKLKEFLPSILEQDYPDFEVIVVNDGSTDETDM
                     LLIDLLKKYPRLRTTFVPMEATNLSTKKLGISLGIKAAKNDFLLFTDADCQPKSKDWI
                     SLMVRNFVPKVEFVLGYGAYFHRKGLLNRLINYDTFFIALQYMGMAIAGKPYMGVGRN
                     LAYRKTTFLHHNGFSESLGLISGDDDLFVNKTANGNNTRVEISPESITFSEPKTTWKS
                     WYLQKARHLSVSPFYTWQSKLMLFMEPFTRDLFYVFCFVLLLQGDMIISLVVAAIFLI
                     RYFTQLYIVNRTGKHFNERRFFLLLPIFDLVLPILSLSIFITGKASRRKNKKW"
     gene            complement(14754..15287)
                     /locus_tag="PLM04_02625"
     CDS             complement(14754..15287)
                     /locus_tag="PLM04_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HON02005.1"
                     /translation="MRIISGRLKGRNIYAPVDISARPTTDFAKEGLFDVLNNRIDFED
                     IEVLDLFAGTGSIGFEFVSRGCRQVVSIENNPKQCLFIKKISEKFEIDNLILLKTDVF
                     KYIAHTASRFDLIFADPPYQLENLKEIPDMIFSQQLLKTEGIFILEHSSKYNFSQHPH
                     FMETRKYGKVHFTFFKA"
     gene            complement(15298..16041)
                     /locus_tag="PLM04_02630"
     CDS             complement(15298..16041)
                     /locus_tag="PLM04_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HON02006.1"
                     /translation="MYSSRGDLESINTYIQPYSLSIRFSPGGLSLSILERDNTKLVSA
                     KRLNMPLFSMTKEQIIPLLLQEPETQLLYDKIHLIAETKTYTIVPKEIFKEEEMDKLL
                     YFSYQKEKNASILYNEISAFDAINIFSLPEDISEVFKQLFPACKIEHSISHFIENHIH
                     KHSLQECICISVRQKIMDVVAFQHNRLLLVNSFDYQTQEDFVYYLLRIIEELNLDLQN
                     CKVYLYNMEQHSKLQGFVRKFVKQCETID"
     gene            complement(16074..16754)
                     /locus_tag="PLM04_02635"
     CDS             complement(16074..16754)
                     /locus_tag="PLM04_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02007.1"
                     /translation="MKTVIKILLSLSIVLLSYLCVASIITPIRFERIKNQREKDVIQC
                     LVDIRKAQIEFKDQNKRYTASFDTLFHFLKTAKKKIVMKEGFLTDKQLEAGLTEAKAA
                     EIVRKGNQKEIAEYGLQNFRRDTILTDLLEEVFPNYNRDNIDKMRIIPYSKNEEFTLK
                     VNNNYTNISGIRIPLFEASALYESYLYDLNRQELLNLIDRTLKLGKFPGLKVGSIEEP
                     NNNAGNWE"
     gene            17235..18053
                     /locus_tag="PLM04_02640"
     CDS             17235..18053
                     /locus_tag="PLM04_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02008.1"
                     /translation="MKKMLCLLLIASTVSQIFPQISSIIPVSSSIAGTSVANTGLRSE
                     FQNPAMLGYAKNSEIGMQHENRYALSELSTNSFQALLSTSAINADLSFSYFGYSLYHE
                     MMAGLGFGRNFSDKFALGVRFNYYMAYFSASNSYRGALLPQIGLSYRFSSLFSLGFST
                     FNPFQTNIKTDFTQKRIPSIFSLGTEYYFSPDLVWLAQIDREISSNYRLATGFEYQML
                     QQVSLKLGAYDAGYLVPCGGCGLKWNRFLLDINCELHPLLGLNSYAGLRYIFSR"
     gene            18077..20149
                     /locus_tag="PLM04_02645"
     CDS             18077..20149
                     /locus_tag="PLM04_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HON02009.1"
                     /translation="MKRIVILVCCLLFLLLNAAYAQDASTTIDQTIADIFEQWSAESE
                     EVPDYQTFYEELEFLAENPINLNHTSREELSKMLFLSDVQVENILAYLYQYGPMENIY
                     ELQLIDGLDMTDIRRMLPFVMVKKEKAVSEKIYRYDVLNYGKNELYMMFNRDAETRQG
                     YQASFSSIGDSTNQGKKYAGSPLYHSAKYRFHFKDRILAGMTFEKDAGESFFGSAHKG
                     YDSYSGYLQINRLGIFKTIVLGDFRANFGQGLVMKSDFSMGKSSYVLNVVSRNEGLKK
                     FSSTDEYNFFRGGGATIRLPRNWETSVFYSNKMIDADTVGGVFSSIYKTGYHRTENEL
                     NKKHTICQQVAGGNISWRLLWLQTGITFVHTNFDQVLVPEKTTYNRFYFSGKAQSTAG
                     WNYRVHWQKLNLFGEAAVTGNFHPALLSGFTLSPLSLVNLVAVYRYYSPEYDTFYALA
                     LSETSRTNNESGLYLGAEINPAKKWKISAYADSYRFPWPKYGIDAPSAGKDYLFQTDF
                     SAHRRLSMFWRFRHSNKPANISQENSVMPVVKAVKKNSLRYQLMYSQGNFSFRTAAEG
                     NMVQKGENADYTYGLTAYQDLTYEFSSLPLKLDFRYQFFDAVAYENRLYAYEKDVLYA
                     FSIPMYYGIGSRYYLNLKYELNRQFTLWFKIAQTVYADDREEISSGNEKILGNKITDV
                     KFLLRFNF"
     gene            complement(20383..20805)
                     /gene="tsaE"
                     /locus_tag="PLM04_02650"
     CDS             complement(20383..20805)
                     /gene="tsaE"
                     /locus_tag="PLM04_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HON02010.1"
                     /translation="MTEIFDLNNIQQPARKFINLFDRYSVFAFYGSMGAGKTTFIKAV
                     CEILGVKDTVNSPTFSIINEYEAADGRVLYHFDCYRINKLQEAIDLGAEDYFYSGKIC
                     FIEWPEKIEALLPDSTVKVNIQEVENGIREIEMILPTI"
     gene            complement(20863..22701)
                     /gene="uvrC"
                     /locus_tag="PLM04_02655"
     CDS             complement(20863..22701)
                     /gene="uvrC"
                     /locus_tag="PLM04_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HON02011.1"
                     /translation="MNLHPQLKQQIDALPEKPGVYQYFNEKEEIIYVGKAKNLKKRVS
                     SYFTKTHESRKTAVLVRNISSLKYIVVDTEEDALLLENNLIKKFQPRYNVLLKDGKTY
                     PSICITNEEFPRVFKTRNIIKNGSLYFGPYSSSYTINSLLEIIHDLYPIRTCKLQLTD
                     ENIKAGKFKVCLQYHIHKCNGPCEGKESAEIYRKYINEIKQIIEGNANEISKMLWEEM
                     QKLSAAYKFEEAYQIKQKYDLIENYKSKSIIANSVVENTDVFGYEETENAAYINILRI
                     SKGSIIQGYTIEYQKHVDEEKEDLLAMAIVELRERLKSNSKEIVVPFEINYKPNGVSI
                     VIPQRGDRKKLLDLAQQNVLQYKFEKLKQAEKLNPDQRSMQLLKEIQEKLHLEKLPIT
                     IECFDNSNIQGKDAVAACVVFKKGRPSKKDYRKYNIKTVVGPDDYASMKEVVKRRYSR
                     MIAENGELPDLIIADGGIGQMEVIREVVEDELNLRIPIIGLAKDSKHHTREILFGFPP
                     KNIGMTPNEPLFKLLATMQEEVHRFAIKFHREKRSKNQTTSELDNIKGIGDKTKRELI
                     NHFKSIKRLQNANLEDIQKVVGNHRASIVYGYFHKDLSAPESQISK"
     gene            complement(22782..24656)
                     /gene="mnmG"
                     /locus_tag="PLM04_02660"
     CDS             complement(22782..24656)
                     /gene="mnmG"
                     /locus_tag="PLM04_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HON02012.1"
                     /translation="MDFKYDIIVIGGGHAGNEAACAAANMGSKTLLITMDMNKIGQMS
                     CNPAVGGIAKGQIVREIDALGGQMGVVTDRSAIQFRMLNRSKGPAMWSPRSQSDRHQF
                     ILQWIQTLTNTPNLYIWQDTVKQILIENGQVTGVLTALGIEISAKCVVLTAGTFLSGL
                     IHIGKNQLIGGRISEPASFGITEQLKSLGFTTGRMKTGTPARIDGRTIDFSVLKEQPG
                     ENDFHKFSFLTEVTRELKQMSCWITYTNELTHDILRQGLPESPLYNGQIKSIGPRYCP
                     SIETKIVTFADKSSHQLFLEPEGVDSQEYYLNGFSSSLPVQVQIQAIHTIPGLEKAEL
                     YRPGYAIEYDFFDPTQLKHTLETKRIKNLFFAGQVNGTTGYEEAAGQGLIAGINAHIN
                     CHGGKEFLLDRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADLRLTPKG
                     YEIGLVTKNRINQYSFKYEAIESLKTFVENFSIKPAQVNGFLVSKNSSELTHGCKLVD
                     LILRPQISIMELSEIVPALKSKIQSIGDRVEEIVEAAEISLKYKGYIDREKSIAEKFH
                     RLEHINIKGKFDYHTISSLSTEARQKLSKIDPETIGQASRIPGISPSDINILLVLMGR
                     "
     gene            complement(24737..25228)
                     /locus_tag="PLM04_02665"
     CDS             complement(24737..25228)
                     /locus_tag="PLM04_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HON02013.1"
                     /translation="MSENFFISSSLDKKSKYEILLQQIPALIADEPDIVANLANLSAV
                     FHQTFKWWWVGFYLVKEDFLVLGPFQGPVACTRIAFGKGVCGTAWKEKRSLLVPDVND
                     FSGHIACSSISVSEIVIPLYNSSSEVVGVLDIDSEKFGVLDEADIYYLEQISTIIGKA
                     LFA"
     gene            25444..28266
                     /locus_tag="PLM04_02670"
     CDS             25444..28266
                     /locus_tag="PLM04_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HON02014.1"
                     /translation="MRKLEFKFFPFVLLICLNTSMFAQQVQFQPLPIDPKVKYGKLEN
                     GLTYYIRSNQQPKNRADFYIVQNVGAILENDDQNGLAHFLEHMAFNGTKNFPGKKIIE
                     YFESVGVKFGANINAYTSLDETVYHLSNVPTNRESIIDSALLVLHDWSNAISLEESEI
                     EAERGVILEEWRQGASADRRMWKESNKQKFPGSQYAKRDVIGDTAVIKHFTPEALRDY
                     YKKWYRPDQQAIIVVGDVDVNKIEEKIIELFSNIKKPENAPERPIYPIYDNKEPIISI
                     VTDPEARMTRVELIYKHDKLPAEIKLSINGYLVNVINSLISNMMQNRFEEITMQPDAP
                     FVAGYATYGELVKSKDAFYLVVVPKEGSETEGIKSVLIEAEKVKRFGFNHSELERAKA
                     DLAKMIEKQYNEKDNQKNESYVREYMEHFLNQDPIPGIEWEYETIKMILPQINADMVN
                     QLAKSYVTDTNMIVSIMAPQKPTVVLPSKEEIRQIIEEVKKTELTAKLDEKIDKPLIE
                     KMPKPGKIKKVSKNDILGTTEWILSNGAKVILKPTTFKKDEILFNAYSIGGLSKVENI
                     EDLPSAEFATEVVSNNGLSDFNQIELKKLLSGKIASVSPYIDNYEEGFSGNSSVSDFE
                     TMIQLLYLHFTALRKDDNAFTALMNNYKAFLANSENNPDKAFSDSVKLMLSNHSPRTV
                     LLNMKTLEKVNQDKALEIFAERFSDPADFTFVFTGNVDPNDKTVVNAINTFIGGIKSK
                     GKKESFTDDNVRLPVGRANNHFSREMQVKKVSNCIVYSGNLNFNMNNRTNMNAIGNIL
                     NIRYLESVREKEGGSYGVGVAANLRNRPIEQAMLFIQFDTNIEKQNKLMDIIHSEIDT
                     IISKGPKAEDLQKVKETMMKKYEEDLQENKWWQSAIKNYYFNKLNVVNDYLPSVQALT
                     SESIQKTLKTIVDQGNIMEIVMKPKE"
     gene            complement(28359..30353)
                     /locus_tag="PLM04_02675"
     CDS             complement(28359..30353)
                     /locus_tag="PLM04_02675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HON02015.1"
                     /translation="MMLMMNGCKSEKKISGIDLTNLDTTARPGDDFYQFACGGWMKKN
                     PLKGEYARYGTFDKLAEENTSQLRELIEEIASKSHVEGTVEQKIGDLYQLAMDSDRLN
                     REGYQPVQTELKKLAGVKTNTELIKILPELLLNGNDVYFSVYVDADPMNSSVYLTQTY
                     QSGISLGERDYYLDQDTHSKEILEKYKQHVAKMFELFGFNSADAQKNRDAVLYIETRL
                     AKASYDRVKLRDPYANYHKMSIQDLSKEFSTISWNEFLHTLGIPAVDSVNVSQKEFLT
                     EVLNILTTVPLDQQIAYLQWKVIDNAAAYLSDSIYNQNFEFYGKTLSGKKEPSPRWKR
                     SVRSIDNFLGEAVGQMYVKKYFPPEAKESMLKLVHNLQESLGQRIQNLTWMGDSTKAK
                     ALEKLGTFYVKIGYPDKWRSYDALQISKNVSYYENVKRAHHFEYAYKFARAGNPVDKS
                     EWLMTPQTVNAYYNPSTNEICFPAGILQPPFFDVHADDAVNYGAIGVVIGHEMTHGFD
                     DQGCQFDKDGNLKNWWTEEDKKQFDARAKVLADYFDSIEVAPGVHANGKFTLGENIAD
                     HGGLQVSFQAYKNSTKGQPSKILDGFTPEQRFFLSYANVWACNIRPEEILVLTKSDPH
                     ALGRWRVNGALPHIQAWYDAFGIKEGDKLYLPVEKRACIW"
     gene            complement(30458..31480)
                     /gene="dusB"
                     /locus_tag="PLM04_02680"
     CDS             complement(30458..31480)
                     /gene="dusB"
                     /locus_tag="PLM04_02680"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HON02016.1"
                     /translation="MKISNIEFPSRPLFLAPMEDVTDADFRLLCRHFGADMVYSEFVS
                     SEALNRNIPRTLQKINVLEEERPVAIQIYGTDPEVMAESAQKVEMIHPDIIDINFGCP
                     VKKVAGKGAGAGLLKDIPRMLKITQFVVKAVNVPVTVKTRLGWDDSSKIIVELAEMLQ
                     DCGIQALTIHGRTRQQMYKGEADWTLIGEVKNNSRIKIPIIGNGDITRPEQAKDFFNR
                     YGVDAIMIGRAAIGQPWIFSEMKHVLDDDFEYSPLSFEDKKAILKNYILSAVRRLDID
                     EQTPIENVYDIHLKGIVHSRRHLAATSIFKNIPNFKSTRIAMLRADTLRELFDIIDSV
                     HETFCN"
     gene            31707..33005
                     /locus_tag="PLM04_02685"
     CDS             31707..33005
                     /locus_tag="PLM04_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HON02017.1"
                     /translation="MENYINKTETYPTERSKIEIMAPAGSWESLAAAIKAGADSVYFG
                     IGKLNMRSKSSSNFSVDDLKKIVEICTENNVKSYLTVNTILYDNDLISMREIIETAKE
                     AGISAIIASDVAAMMYANSLNVEVHLSTQLNISNIEALKFYAQYADVVVLARELNLKQ
                     VSEIFSAIQTKNICGRNGKLIRIEMFCHGALCMAVSGKCYLSLHEKNLSANRGECNQI
                     CRRGYLVKDKDSEIELEIDNEYIMSPKDLKTIEFVDEMLKAGVSVFKIEGRARGAEYV
                     KTVVSCYKEAINACYEGTFSKEKINDWNRRLSKVFNRGFWNGYYLGQRLGEWSKNYGS
                     AATHKKIYIGKVTNYFNKIGVAEFLLEAQSLSVGDEILITGETTGAYEDIVNEIRVDL
                     QPVEKVEKGTYFSMKTNELVRRNDKLFKIVPTEHGKQEGK"
     gene            complement(33038..33961)
                     /locus_tag="PLM04_02690"
     CDS             complement(33038..33961)
                     /locus_tag="PLM04_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HON02018.1"
                     /translation="MTQVTDKVNTSGSKNTQSNVVVDWFIRLIKGIIVGIGFILPGLS
                     GGVLAVILGIYDRIIKFLSDLRKDFVQNVFYFIPVVIGGAIGIVLFSILVEKAFGNYA
                     AQFICLFVGFVGGTFPSLYRTAGKQGRRTKDIIIFIFSTFFIFGLMLLGGQQLTEVAP
                     NIPVWLGSGALIGLGVIVPGMSPSNFLIYFGLYDKMATGIKDFDFAVIIPLLVGFAVC
                     VLLFAKLAAYLFRKFYPEMYHFILGMVVGSSLAIFPTVVFPAFRSEQLSVAGLSFVGA
                     FLLCIGFLAVGVVISFLFSKLEDKFPREEIF"
     gene            complement(34098..34910)
                     /locus_tag="PLM04_02695"
     CDS             complement(34098..34910)
                     /locus_tag="PLM04_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HON02019.1"
                     /translation="MILPIYFLPLAGLLIGILISLMGGGGGIFYIAILTGLFSIPMNQ
                     AVSVSLATIIPTTFAASISHFKAGNVNINVGLILSAGGIIGVIAGSFLLGLLSLNVLK
                     IVFGLFMIFMAVQMIFKNFRSKKSFNKGNIILVEEDKTVNIAKKKKIFSFKNFKGLSF
                     GFFGGIMSGLLGVSGTPPILAGLYNMKLSSVQVVGTSVMVLFFIALSGVITHTAFGHL
                     NWYLVGLLAAGTITGAILGPILGKKIDEKKLEKFYTPFFIVFIFLMGISMFF"
     gene            complement(34939..35868)
                     /gene="nadA"
                     /locus_tag="PLM04_02700"
     CDS             complement(34939..35868)
                     /gene="nadA"
                     /locus_tag="PLM04_02700"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HON02020.1"
                     /translation="MEKKELIEKINKLRKDKNAVIMAHFYQEPDIQDIADHVGDSLAL
                     AQWAAQTDADIIVLCGVHFMGETAKILNPQKKVLIPDMNAGCSLADSCPADAFEEFVK
                     SHPDYTVISYVNTSAAVKALTDVVVTSTNAKKIVESFPKEEKIIFAPDKNLGNYINSI
                     TGRQMLLWNGACHVHEQFSVQKLVELKKKYPEALVLAHPECTKPLLMLADFIGSTQAL
                     LNFATSSDKKFFLVATESGILHEMQKKNPDKQFIPVPPNDSTCACNECNFMRLNTLEK
                     VYQCLLNESPEILVEEEIRQKAVKPILRMLELS"
     gene            36363..37868
                     /locus_tag="PLM04_02705"
     CDS             36363..37868
                     /locus_tag="PLM04_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HON02021.1"
                     /translation="MKIFSTSVVRRLDEYTIKKEPIASIDLMERAAVGLCKKFLEIFP
                     SPLPVCIFAGPGNNGGDALALARMLLQKNYYVTVYLIHTGKLSADCEANKIRLIEKFP
                     GCLHDFSTDFYPVNFSENTVIIDGLFGTGLSRPLSGIYSEAVGWINNSGKTVVSIDIP
                     SGLEGEENKDLSQPIVKADYTFSLQFPKLSFFFAENESFVGKWEIIEIDIHPEAIRRE
                     PTDFYFLEKKDVIPFLPERKKFSHKGTYGHALIVAGSVGMAGAAVLSAQAAIRSGTGL
                     VSVHSAATNRIILQTKVPEALFESDKEENFISELKNLEKYNTLAIGPGIGTQEKTANF
                     LFQILPSIKQSMVLDADALNIMSRKKELLEKLSHNTLLTPHPKEFERLFGADKNSYDR
                     LLKMQQLAKNLHLVIILKGAYTRIAFPDGSIYFNSTGNPGMATGGTGDVLTGILAGLL
                     AQNLSPEKAALLGVYLHGLAGDLALRHQSMESLTAGDVIENLGSAFQSLRE"
     gene            37996..39282
                     /locus_tag="PLM04_02710"
     CDS             37996..39282
                     /locus_tag="PLM04_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HON02022.1"
                     /translation="MTKKILIFLFLSFSIVTFGQTLNLDTCQKRAKENYPLLKQYGLI
                     EKTADYNISNANKAYLPQVSLLAKATYQSDVTAIPETLGQVLTQLSGQPVSFPVLSKD
                     QYQAALEVDQLVWDGGMVNAQKKLIKASSEAEKQKLEVDLYALNDRINNLFFGIMLIK
                     EQLKVNQILQQELASNYQRMEAYLKNGLIQQADLDAVKVEQINAAQQETDLKSVLKSY
                     CQMLSVFTGINVNEKTELEKPLLPVVNENIENKRPELSLFESQKNLLESQKKTILAGN
                     LPKIGLFIQGGYGRPGFDFFKNDFTSFYIGGVRLSWNIAGFYSQKNELKKIETGQKNV
                     DIMKETFLFNTNLLTKQQMNEIDKLRVTLKNDEEIIRLRNNIKKATESKFQNGTITTT
                     DFLREINAENIARQTRTLHEVQLYMAVYQLKNTINN"
     gene            39306..>40068
                     /locus_tag="PLM04_02715"
     CDS             39306..>40068
                     /locus_tag="PLM04_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HON02023.1"
                     /translation="MEAIKFDNKILVLSMSLILGLTSCHRLLENKYDASGTFEATEVV
                     VSAEANGKIMDFNLEEGQQLTENQLIGYIDSTQLYLTKLQLLASQKSVRSRVPDIKKQ
                     IAVLQQQIETAKKEQKRIQNLFSANAASQKQLDDVNAQVSVLEKQLEAAKSNLESTSN
                     SILNENEALNIQIEQVQNQLEKCRITSPINGTVLVKYAEKGELAGAGKPLFKVADLQK
                     MILRAYVTADQVTQLKIGQKAKVFVDFGKDDQKEYE"
BASE COUNT        13053 a         7131 c         7405 g        12479 t
ORIGIN      
        1 acggattacc taaagtctgg aagagaacaa ttttatcttt ttggtcagca aatagcttga
       61 ttacttcaga ggtttgtttt tcttcacaaa ttatggtttt accttgagag ataattaaat
      121 taattcgttc tttaatttta tcgaccaagc tttgtgtgag aggttcatcg atgttgttca
      181 gtacgcaata ataacctttc gataaaggat gttcgatgcg aatgttgcct ttggggaata
      241 attcagaaac ggctttggca actaccattg tcagcgtgcg aacataaacg cgcatgcccg
      301 aagaggtgct taaatcaatg aactcaatgt ctttgggctt ataaatcaaa aaatttagat
      361 cttcgacctt ataatttacg cgggctgcga caacctgata tttcaattga atgtttaacg
      421 accggtaaat ttcaattagt gaagttccca aggggaaatc gtgatatgaa cgggtatttt
      481 tgcaataaat ggttacggtt tttcccatga gtaagataaa aaaattgttt tcaatccaat
      541 ttaaaatatg tcggtaaaat tatatatttt tttttgcatt tctttcaaga aaaaattttt
      601 taaagtactt ttgcaaaaaa aatctgtaat tagaaataaa atgcattaca cattttatga
      661 tttacttaca ttaattggtt cattggggtt gtttctttat ggaatgaaaa ccatgagcga
      721 aggactgcaa aaagctaccg gcgacacgct ccgttccata atggctgcaa tgacaagaaa
      781 tcgtttcacg gggattctta ccgggttgct tattacctca cttattcagt cttcttctgc
      841 cactactgta atggtggtta gttttgtaaa tgcgggatta ttgtctcttt atcaagcaat
      901 aaccgtcatt atgggagcaa acataggaac aaccataacg gcatggatga tttcgatttt
      961 tggatttaaa gttagtatcg ctgctttttc tattccattg ataggcattt ccatcccgct
     1021 tattttctcc tctaaaagtc gcaataaatc cataggcgaa tttttaattg gatttgcctt
     1081 attgtttatg ggtttggagt tattgaaaaa ttcagtcccc gatattcagg gcaatccgga
     1141 agtcttaaat tttcttgcaa aatatacaaa ttttgggttt ggttcgatat tactgttttt
     1201 actcataggc accatgctga ccattatagt tcaatcatcg agtgctacta tggctataac
     1261 attaatcata tgcagcaaag ggtggatttc ttatgaactt ggagctgcca tggtattggg
     1321 tgaaaatata ggaacaacta ttacggccaa tctggcagct attcctacaa atgtttcagc
     1381 taaaagagcc gccttatctc atttaatctt caacattttt ggtgttattt ggatgttgat
     1441 cgctttcagg ccattcatca atttggttac aatgttgata actcaatttg gaccgggcga
     1501 ccctactcaa ttaactgctt tctccaatag ccttgaccca gatacgctaa aagttatttc
     1561 ttcgggaagt gaaaatcttt cacccgaaca aattgcagtc aggaatcaac tagaaaatta
     1621 tcaaatagca acctcatacg gcttgtcatt atttcatacc ttattcaaca taaccaatac
     1681 tttggttttg atatggtttg ctaaaattat agagaaaatt tcaaccttgc taataaaaaa
     1741 gaaagatacg gatgaagaat ttcagcttca atttatttct accggattgc tttctacttc
     1801 agaactttca cttatacagg catggaaaga gattaaggtt tatgctgaac gttcccaccg
     1861 tatgttttca ttggtaagag aattgcttca agaaaaaaat gaaaatgatt ttataaaaaa
     1921 atttagtcga gttcagaaat atgaaaatat aagcgaccga atggaagttg aaatagccca
     1981 ctaccttacg aaagtggccg aaggcaaatt aagtgaaaag gggaaatttc agatacaaac
     2041 catgttgaaa gtaatttcgg aaattgagag tattggtgat agttgttaca acttggccag
     2101 aactatcaag cgaaaaaaag acgacaaagc tgaatatacc aaagaaatgg atgataattt
     2161 gaatgaaatg atgaatttgg tagaagatgc cctcttacaa atgcgagcag ctatcgacaa
     2221 caaccatgta tcaaaagaag aattgaatcg cgctgaaaat ttggaaaacg aaatcaataa
     2281 tttcaggaat caactcaaaa ataaaaatat tgatgacgtt aaagaaaaca aatacgatta
     2341 tcaggcaagc gttacataca tggatatgct tgaagaatgc gaaaaacttg gagattatat
     2401 cgtaaatata gttgaagcac tttacgaagc ttccaaataa atgacgaaag gaatcaaatt
     2461 ccatgtttaa tccgtttcca tctctgccga tccaccaaaa gtaatgttat ccatttttct
     2521 gcctcgcaca aaatttcttt tcataaaaaa gccgtaattt tgcttttgaa attgacaagt
     2581 ttttcaactc tttctttttt atatcaataa cttgaaaagt cgaacgaaaa aaattatcgt
     2641 ctaacactac tctcatttaa aatggattac atattgatta tttcgggttt tgctgttttg
     2701 gttttgggtg ctaacttatt agttgatggt gcaagcagct tggccaagaa attaaagata
     2761 tccgatttga tcatcggact tactgtggtg gcttttggga cttccgcccc tgaactggtg
     2821 gtaaatgtaa tagcttctgt gaaacctaat gcaacagata tagcaatttc caatgttatt
     2881 gggagcaata ttgttaatat ctttatcatt cttggatttt cagctctgtt tcatcccatt
     2941 aagtcggaga aaactacaac tttatttgat atacccttaa gcttttttgc cattgttgtt
     3001 acatatcttc ttgcttttac gggagatagg attttatcaa gaaccgatgg acttgttatg
     3061 cttggttttt ttatcgcttt catatatttt accacccgca ggtcgataaa acatccattg
     3121 atacctttgg aagaaaacat caaaattcgt aaaacgggaa tttcgatttt gatgatttct
     3181 tgtggattgg cagcccttat aggtggggct gaaattattg tacccagcgc cattcgtata
     3241 gcagaaaatt tgggtatcag tcaagcagtc attggattga ccatcgttgc attgggaaca
     3301 tcgctccctg agttggccac ctctgtagtt gctgctatca aaaagaattc cgatattgca
     3361 cttgggaata ttattggctc aaatattttt aatattttcc tgattttggg tgtgagcagt
     3421 gttattcgcc cattacccat ctataggggc ataggaatag acttgactat gggtgcactt
     3481 ggcagtgtga tggtttttgt ttttgttttt ggaagtaagt cccacagcat aaaaaaatgg
     3541 caaggtttat tgctgatttt catatatcta atgtacgcaa ttttcaaaat tttttaattt
     3601 aaagtatcct taaaaaatta tttaacaact cctcgttatg aatctttttg atgtgtatcc
     3661 gctttttgat atagaaattg ttaaaggaca gggatgtcgt atttacgaca aaaacgatac
     3721 ggaatatctg gatttttatg gaggtcatgc cgtcatttcc attgggcatt cgcatcccta
     3781 ttttgtaaaa aagctggcgg atcagattca gaaactggtt ttttattcca attctgtgaa
     3841 aaacagttta caggttgaat tggccgaaaa gctgggaaaa atagctggtt acgatgatta
     3901 ttccctgttt cttattaatt cgggcgcaga agcaaacgag aatgcgttga aactggcttc
     3961 gtttcacaat ggacgcagca aggtgattgc tttcggaaaa agttttcatg gcagaacttc
     4021 ggctgcggtc ggagttactg acaatccagc cattcaggcg cctattaatc gaaattttga
     4081 ggtagaattt ctaccgttga acgatataga ggcagttctt caatctttga aaaacagaga
     4141 tgtttgtgcg gtgattattg aaggcattca aggagtagga gggattcaga ttccacaacc
     4201 ggcgtttctt gaagcgatta gcgcagcctg caaggagacc ggaaccgtac ttattctgga
     4261 cgagattcaa tcgggttatg ggcgaagtgg aaaatttttc gctcatcaat atgccgatat
     4321 tcgccccgat attattacca ctgcgaaagg catgggaaac ggagttccga taggtggagt
     4381 aattatcagc ccaatgttta agcctactta cggaatgttg ggaaccactt ttggaggaaa
     4441 tcatcttgcc tgtaccgctg ccattgctgt attggatgtt attgaacagg aacatcttat
     4501 ggaaaatgcc ttgcgggtgg gaaattattt gataaacgaa ctccaaaaat tttcccgatt
     4561 gaaagaagtc agaggaaaag gtttgatgat agggattgaa tttgacgaac cggtaaaaaa
     4621 attgagagaa cgtttattgt ttgagaaaaa aatatttaca ggtgtgagtg gttccaatat
     4681 tatccgtttg ttgccgcctc tttgtctgac tatgcaggat gcagaacttt tccttgatca
     4741 ttttgctgaa gtgataaaat aattttgtaa tttctcttag tttcatgaaa aaaccgatag
     4801 cccttttgtt tatccttttt tttgtaaata gttggactgt tttctctcag tctgtttcaa
     4861 aagaacggga taaagtcgtg atgaaaaccg gtgaaattta ttacggagaa gttgtttttc
     4921 gaaatgacga aatgcttatt ttgcaaacag acgaaggagt tcgatatcag ttttctattg
     4981 cggaagtgaa agccatttac aaagaaacgg tagatggttc tgatgagtta gaaaaaatgc
     5041 aacaggaagg tcagtctgat gaaatacaga acttttttgc agtgttggat gcgtgtggag
     5101 gaatctcgag tgccaaaaac agttttgatg tttcttctaa tactcaagta gcatttgttt
     5161 tggggaaaga taaggttttt tcggaaaatt ttttcgtggg tttgggtgtc gggtacaatt
     5221 cttcttattt aaagagtaat tcgtcactga attttctccc cgtttttctt cgcttgcaaa
     5281 atacttttac taacaatcgg atttcgcctt ttgttcgcat ggatgcagga tacgcttttt
     5341 ctatgacttc cggctgggat ggaggtgcat tggttaaagc ggctgccggt atcagttcac
     5401 gtttaagtta taaaacaaaa cttttctgtg gaatttatgc cggtattcag caatttggtg
     5461 gaaagctttc cgaaactatt tcttcaggca cttattatta cgaaggtaca acaaacatga
     5521 ggcaattggg tgtgcaactc gggattcaat tttaaattca gcaaaagcag aaatttttca
     5581 actataatta aattgctgat aatgaataaa ataaatttat ctcaagttat aattgcagca
     5641 ttaaatgaac aggcgtgtgc agaaaaagct cagcatcacc tccggttttt caaaactgct
     5701 ccgggcgaat atggcgaagg agacaaattt attggtgtga atgttcccga tcagcgaaaa
     5761 attgccaaaa aatatttcag agaaattaat ttgaatgagc tggaagtttt attaacgaat
     5821 gaaattcacg aatgccgttc tacagctctt tttatgcttt gttacaaatt tgacaaagcc
     5881 ggtgaagcgg aaagaacttt gatggcggac ttatacctca ataacctatc ttttgtaaat
     5941 aattgggatc tggtagatgc ttctgctgca aatattttgg gttgttactt gatggacaaa
     6001 aaaagagact tgctttacga ctttgttcaa agtaacgatc tttggaaaca gcgcattgcc
     6061 atcatcgcta cgttttattt tattcgttat ggtgagtttg aggacacttt caaaatttcc
     6121 gaaatgctgc ttcatcatcc tcacgacttg attcataaag ccgttggatg gatgcttaga
     6181 gaagtgggga acagagataa ggaagcagaa atcaactttt tactaaagca ttatcggcaa
     6241 atgcccagaa ccatgcttcg gtatgccatc gaaaaatttg atgaaggttt gcgccggcaa
     6301 tttttaaaag ggtaaattta cctataaatt cctattgcat tcgttcaatt ttattaggtg
     6361 tgatcaaaga tgcttttcct actttttata gagtcggact ttattttttc gtcggggaaa
     6421 aaagattatc caaagtccgg agatattatt ttaattctgg agcgttattt aaaaaattat
     6481 ggagtaattt tgtgagttca aatgaatgct tcttcatgaa agcgacttaa taattttccg
     6541 caaaatgaaa ttaaaagaaa ttgcccaaat acttgggaat tcacatttaa aagttcctga
     6601 agttgaaata aattggttat tgaccgatag ccgagctctt tcgtttccaa caagaagttt
     6661 gttttttgct ttgcgtaccc ggcgaaacga cggacatcgt tacatacagg aactttatga
     6721 ccagcattta agatattttg tggtcagtga aacgcttcca gaatttgaaa aaatgcacga
     6781 tgccattttc attcgggtaa acgatacgct ggaagctttg cagcatttgg ccgcagttca
     6841 cagaaatgct tttcaaattc caattgtggg aataacgggg agcaatggta aaaccattgt
     6901 caaggaatgg ttgtatcaac tgcttcattc cgatttcaac attacccgat cgccacgaag
     6961 ttacaattct caaatcggtg tgccgctatc ggtttgggga ttgaacaaca ataccgagct
     7021 tggaattttt gaagccggta tttctcaacc gcacgaaatg gaacgtttgg aaccaattat
     7081 tcgaccgacc atcggaattt ttaccaatct cggcgatgca catcaggaga atttttccag
     7141 cttgaaacaa aaatgcgaag aaaagcttaa actttttgcc catgctcaag ttttgattta
     7201 ttgttcggat aatagattgg tggatattac ggttgctcag tcgaatttca aaggcaaatt
     7261 attttcttgg ggaagaaatg aaaatgctgt tttacaactt gttaaaacaa aaaaactatc
     7321 cgattcaacg gaagtagttt ataaattttc aggaaaggaa tcggctgttt ccattccttt
     7381 ttccgacgat gcttctatag aaaatgccat gcattgcgta gcattgatgt tacatctcgg
     7441 tatcgatttg gaaaaaattc gtcaacgcat agcgaaactc gaaagtgtgg aaatgcgcct
     7501 tgaattgaag gaggggattc acggatgcct tttgattaac gacagttata attccgattt
     7561 gaattcgttg gcaattgcgt tggattttct caatcagcag gctgcaacca aaaatttgcc
     7621 gcgaacgttg attctttccg acattttgga aagtgggaaa cagtcggatg atttatataa
     7681 tgccgtagct cagttggtta aaagtaaaag catactgcgg tttgttggta taggcccaga
     7741 aattgtcaaa catgccgata aatttgaggt agagcaacaa aattttttcg aatttacgga
     7801 agattttttg aagtcgccac ttatccacga attgcagaaa gaaatcatat tattgaaagg
     7861 ttcacggaaa tttcattttg aaaaaatttc cgaaaagttg gaactgattg cttacgaaac
     7921 cacattagaa gtaaatttga atgctttgat caacaacttc aactattttc gttccaaact
     7981 ccgtcccgaa accaaagtta tgtctatggt aaaagctttt gcctatggaa gcggatcggt
     8041 agaagttgca cgaaccttgc aactccatca ttgcgattat ttggctgtag cagtagccga
     8101 cgaaggagcc gaattgcgcc gggaaggaat tcgtatcccg atagtggtta tgaatcccga
     8161 aaaaggaagt ttcggaatga ttttcgagca tcaacttgag cctgaaattt acagtttcag
     8221 aattttggaa tcatttctgt cggcagccga aaaattgggc gtaaccgatt atccggttca
     8281 cataaaaatt gacagcggca tgcatcgatt gggatttgaa cctcacgaaa ttgttccttt
     8341 gatacaacgg ctgaaagcac aaaacaacct gaaagtgcgg tcggtatttt ctcatttggc
     8401 cggtgccgac gaaaaacgtt tcgatgattt tacgcggcaa caggtaaaag ttttcactga
     8461 atgtgccgat ttgtttgctc agtcgtttga gcatcccatt ataaggcata ttctcaactc
     8521 tgccggcata gaacgtttcc ccgaatttca atttgacatg gtgcgtttgg gaatcgggca
     8581 ctatggtgtc agtgcgttgc ccaaagttaa actggaacct gtttgtgcac tgaaatccat
     8641 tattttacag attaaaaatg taaaaaaagg agaaacggtc ggttatagtc gcaaaggaat
     8701 tgccaacgaa gacaaacgaa ttgccgtcat tccggtgggt tatgctgatg gcttcgacag
     8761 aaaattggga aatggtgtcg gcgaagtttt gattaacgga aaacgcgcca aagtgatggg
     8821 taatgtatgt atggatttga caatggttga tattaccggt attgaagcaa aagagggaga
     8881 ttcggtggaa atattcggca acaacctgtc tgtcatcgaa gttgccgaca agttgaaaac
     8941 cattccctac gaagtgctca ccagtgtttc caaacgagtg aaaagagttt attttcaaga
     9001 ataaattcag tctgttttgg tatttaagtt gagttttatt ttattttatt ttaaaaatta
     9061 gatgatcctt atttttgtac aaaatacaac cccgccggtt gcacttttgc aaagtgttgc
     9121 catattgtct gtccaattga aaaaagaatg gggcatactt tgtttgacgg aaaatgccga
     9181 tttggtttca gtttacgaaa atcaatatgg tgaattttta aaaacaaacg gattcccgga
     9241 agtgtctata tttgtgagac aagcttgttg tgagcaactt gtagaatttt gtgaacaact
     9301 ggaagcttct tttctttttt ttcagttgca tgatgcatcc caccgatatc ttcgtcggtc
     9361 gttaaatttt tgtcgtgatt tgcgtatacc gtatttgttt ttcaaaaatt ccttttcggt
     9421 gcttaatttg gagaaagttg tagttccgat aggttttttg gaagaagaaa tagaaaaagc
     9481 gcagtttgca tctgcttttg ggcgatttag caatgctgaa atcattttgc tccctgccaa
     9541 cgattatggg tctaaagctg caacgacaac cgataaaatg aaaactatcc tcgacaagtt
     9601 tcaattgccc tacacggttg agaaagcaca atcggacagt tttaaaattg acagagaagc
     9661 aatagaaaaa gcagttgaaa tgggaggagg gttagtcatc atttctgcgt cgcgagatta
     9721 cgggctggat gatattcttt ttgggcccaa agaattgcac ttgatacgga aatctcccgt
     9781 gccgctttta ctggttaatc cgcgaagcga tttgtatgct ctttgcgact gaaaaatttt
     9841 tcagtgtttt atttcaaaaa aagttcccca aaaaactccg gacgatggaa atcgggtttt
     9901 tcggttttta tgggattcca acttaaataa tgtggcgatt cagttccatc ggcgcatttg
     9961 taaaaatttc ccagaatttt ttcgggtaaa ttattcgggt cgatgcctat cagtttaaag
    10021 ggaattttta ctgtcaactc ccagtcgaaa tttcctttta tttcctcaaa aggctgtggc
    10081 acgatggaag ggaatctttc aatggatttc atttctgttt ccgaaagagg ttcaacttca
    10141 tcgtttcggc ctttacgccg ggaagccgag caagtcccaa tgcaattgaa ttcaaaatta
    10201 atatatttct cactctcgat agttttgcag aaaaattcca cacaactgtc ttcgtaaacc
    10261 gggtcctggt cgtgcgtgta aacggcacgc agcgcattgc acaaaacaaa gaatttgata
    10321 aatacagctt ctttcgactg cgcaatgtgg aaataagcaa tgggtttgta gggaaactct
    10381 tttgcccagt tttgggattc aatggcggct tttgttccgt ttttttccag tatttcgctc
    10441 gctgttgtta tgtctgcttt gtctaaatcc ggaatataac cgatatgcaa tgatttcatc
    10501 agaatataat ttatttttta aaggtctgat ggaacccaca tgtctgaata ttatattcat
    10561 tcgtgtttgt gtatgcaata catgtgggtt tcataatttt gttttctttc aggagaatta
    10621 cgtcttacaa agataacttt cttttttaac aaatcaattt caaaaatgtt tcccgcattg
    10681 aaatttattt tcctttgcat aaagcacatt ggtagcttgg aagttgaatc cctgcataca
    10741 aaaatgtcag aaaattgtat tatttttgtg gaaaatttca gaaataaata ggaatggctt
    10801 cattctatta aaaataactg atttttaaaa ctattttaag aacattaagt aggtaaggga
    10861 aacgaaaaat ttatcttttt tgaatcattt ttcggcttta ccaacaatta caatataaaa
    10921 ccagacaatt atgaaagtat tgaaatttgg cgggacttcg gtaggttcgg cacagcgcat
    10981 gaaaaacgtt gcaaaactga tttgcaatgg cgagaaaaaa attgtggttt tgtcagctat
    11041 gtctggaacc accaacagct tgcttgaaat tgcggattat ttttacaaaa gtaatcttga
    11101 cggagctttg gaacatatca ttacgctgga aaacaaatac agcgatgtgg tggaagaatt
    11161 gttgacaacc gaagaaaaac ggcaggaagc ccgttctatt ctttcgtctc atttcgatta
    11221 tctgcggtcg tttgccaaat cggtttttac cattttcgaa gaaaaagcca ttgtagctca
    11281 gggagaattg ctttcaaccg cactttttca actttatctc gaagaaattg gtgaaaattc
    11341 catacttctg ccggcgctcg aatttatgcg tatcgacaaa aatgcagaac cggacaatgc
    11401 ctatatcaaa cagcatttga acgatttgct cgaagcgcat cccggtcatg aaatatatct
    11461 tacgcaagga tttatttgcc gcaatgctta tggcgaaatc gacaacctgc aaagaggagg
    11521 aagcgattat acggcttctt tgattggcgc tgccgtaaat gctgaagaaa ttcaaatctg
    11581 gacagatatt gatggcatgc acaataacga tccgcgtttt gtggaaagta ccaaacccat
    11641 tcccatgctt cattttgaag aagcagccga attggcttac tttggtgcaa aaattcttca
    11701 tcctacctgt attttgcctg ctaagttggc taatattccc gttcggttgc tcaatacaat
    11761 ggaacctgaa gctgccggaa cgcttatttc tacacggatg aataagggaa aaatagaagc
    11821 tgtggctgca aaggatggaa ttacggcaat aaaaattcag tcgggacgaa tgctgttggc
    11881 ttatggtttt cttcgcaagg tgtttgaaat ttttgaatcc tataaaacgc ctatcgatat
    11941 gattactaca tcggaagtgg gcgtttcggt tacaattgac aacacgaaaa aattggacga
    12001 aattgtcaat gatttgaaaa aacttggcac cgtaactgtt gatcacgata tggttatcgt
    12061 ttgtgtagta ggcgatcttc ctttcgaaaa tgtcggcttt caggcaaagg ttgttgatgc
    12121 attgaaagat atacctcttc gaatgatttc gtatggcggc agcaattaca atatttcatt
    12181 tttgataaaa gctgaagaca aaaaacgtgc tttaaacgct ttgagtcagg ctcttttcag
    12241 tgaagaatag caattcccga aacctgaaag agaacgtttt ttatattttt atttgtatga
    12301 taaaagcaaa atatcctgtt gaaaaatttt taaaactgca aacgccgttt tattattacg
    12361 acatggacat cctgcggcaa acgcttcagg aagtgaatcg acagattcag aaaagaaact
    12421 atgtggttca ttatgcgatg aaagccaatg caaatcccgt attgcttcat gaaataaaaa
    12481 atgccggttt gggggccgat tgtgtgagcg gaggtgaaat aaatgctgcc attaaagcag
    12541 gatttccgaa agaaaaaata gtttttgccg gagttggaaa agccgactgg gagatcaatc
    12601 ttgcgttgga tgaaaacatt ttttgtttta atgttgagtc gattcccgaa atggaagtta
    12661 tcaatcaatt ggctgctgcc aaaagtaaaa cagcttctat cgccttgcgt atcaatccca
    12721 atgtaaatgc ccatactcac caatatatta cgaccggcct caacgaaaac aaatttggaa
    12781 ttaacctttc cgatttggat acggttttgg acttgctccc acagttgaaa aatttaaaat
    12841 taatcggtat tcatgcgcat attggctcac aaatcaccga tatgacatcc tttcagagtt
    12901 tgtgcgtgag aatcaacgaa attcagaatt atttttttca gcgccacatc attttggagc
    12961 atattaatgt aggtggaggt ctgggtatta attacgaaca tcccaatcat tttccgatac
    13021 ctgattttga gagttatttc aatgtatttg ccgaacattt gaatcttcag ccgtatcaaa
    13081 cactacattt cgagctggga cgtgccattg tagcgcattg cgggagtttg atttcgaggg
    13141 tgctttatgt caaacaggga gctaccaaaa aatttgtcat tctggacgcc gggttcacgg
    13201 atcttatacg tccggcttta tatcaggctt atcatcgtat cgaaaatttg acttccgatt
    13261 tgcctgaaga agaatatgac gtggtcgggc caatttgtga atcttcggat actttcgcca
    13321 agaattattc tttgaatgga acacgcagag gcgatttggt ggcattgcgt tctgccggcg
    13381 cctatggaga aataatggct tcacagtata atttgcgctc tttgccgaaa gcttatacat
    13441 cggatgaaat tgctgcattg taaaaacagc cgatttgatg tttttctttt aaaaatttta
    13501 gtgaaaaaac catttactga actttaggaa aaaatcggcc atcaattttg aaaaatacgc
    13561 ttacccaaat gacagaaata ttcggatttt cactttcttt gttgacactt gttttattgt
    13621 gcggactgtt tttggttttt tgttatcaga tctattttta ttgtcgttat atcaatgggg
    13681 tgattcgtta ccggaataaa atcagaaagg ggaaagttgc ttttctggat gaaaaacccg
    13741 gtgtttccat aattatttgt tcaaaaaacg aagccaacaa actgaaagaa tttcttccgt
    13801 ccattcttga acaggattat cccgattttg aggtgattgt ggtgaacgat ggctcaaccg
    13861 acgaaaccga catgttgttg atcgatttgt tgaaaaaata tcctcgtttg cggacaactt
    13921 ttgtaccaat ggaagcaacc aatttgagta caaaaaaatt agggatatct ttgggcatca
    13981 aggcagcaaa aaatgatttt cttcttttta ccgatgccga ttgtcagccc aaaagtaaag
    14041 actggatttc gttaatggta agaaattttg ttccaaaagt tgagtttgtt ctcggatatg
    14101 gagcttattt tcaccgaaaa ggcttgttga acaggcttat aaattacgat acttttttca
    14161 ttgcgttgca gtacatggga atggccatag caggcaaacc ttacatgggt gtgggaagaa
    14221 atttggctta tcgtaaaacc acttttttac accacaatgg tttttcggaa agtttaggat
    14281 taatttcggg ggatgatgat ttatttgtga acaaaactgc caacggaaac aatacacggg
    14341 ttgaaatttc gcccgaaagc atcacttttt ccgaaccaaa aaccacatgg aaaagctggt
    14401 atttgcagaa agcccgacat ttgtccgttt cgcctttcta tacttggcaa agcaaactga
    14461 tgctttttat ggaacctttc acacgcgact tgttttatgt tttttgtttt gttttgttgt
    14521 tgcagggtga catgataatt tctctggtgg tggctgccat ttttttaatt cgttatttca
    14581 cacaacttta tattgtcaat cgtacaggaa agcattttaa cgaacgcagg tttttcctgc
    14641 ttttaccgat ttttgacctt gttcttccca tcctttcgct cagtattttt atcactggaa
    14701 aagcgtcccg aagaaaaaac aaaaaatggt aatttctgaa agattggctc ttttcaggct
    14761 ttgaaaaaag tgaaatgcac ttttccatat tttcgggttt ccatgaaatg gggatgttgc
    14821 gaaaagttgt atttggatga atgttcgaga atgaaaattc cttccgtttt caacaattgc
    14881 tgtgaaaaaa tcatatccgg aatttctttc agattttcca gctggtaggg aggatcggca
    14941 aaaatcaagt caaatcgtga ggctgtgtgc gcaatgtatt tgaaaacgtc ggttttcaac
    15001 aaaatcaggt tgtcaatttc aaatttttct gaaatttttt ttataaaaag gcattgtttt
    15061 ggattgtttt caatgctgac gacctgacga caacctcttg acacaaattc aaaaccaatg
    15121 ctgcctgttc cggcaaacaa atcgagtact tcaatgtctt caaaatcaat tctgttgttc
    15181 aatacatcaa acaaaccttc ttttgcaaag tcggtagttg gacgtgccga aatgtcaacc
    15241 ggtgcataaa tatttcttcc tttgagtcta ccgctgataa ttctcattat atattactta
    15301 atcaattgtt tcacattgtt ttacgaattt tctgacaaat ccttgcagtt ttgaatgctg
    15361 ttccatgttg taaagataaa ccttacaatt ctgcaagtcc agattcaatt cttctatgat
    15421 tctcaaaaga taatacacaa aatcttcctg cgtttgatag tcgaaactgt ttaccaacaa
    15481 aagtcggttg tgttgaaaag ctaccacgtc catgattttc tgccgtaccg atatacaaat
    15541 gcattcttgc agcgaatgtt tgtgaatgtg gttttcaata aaatgagaaa tggaatgctc
    15601 tattttgcaa gcagggaaaa gctgtttgaa aacttccgaa atgtcctcag gcaaagaaaa
    15661 aatgttgatg gcatcaaaag ctgagatttc attgtataat atagaagcat ttttttcctt
    15721 ttgataagaa aagtaaagaa gtttgtccat ttcttcttcc ttaaaaattt ctttgggtac
    15781 aatggtgtac gttttggttt cggcaatcaa atgaattttg tcgtaaagca gttgagtttc
    15841 gggttcttgc agcagcaaag gaataatttg ttcctttgtc atggagaaaa gcggcatatt
    15901 caatcttttt gcagaaacaa gttttgtgtt gtctctctct agaatagaca aagaaagtcc
    15961 acccggtgaa aaccggatgg acaaactata aggttgtata taggtattta tagattctaa
    16021 gtcgccgcgg gatgaataca ttttttcatt tatctctgaa agacttacag gatttattcc
    16081 cagtttcctg cattattatt gggttcttca atagaaccga ctttcagacc tggaaattta
    16141 cccagcttta aggttcgatc tatcaaatta agtaattcct gacgattcag atcgtaaaga
    16201 taactttcat agagagcact ggcttcaaac aatggaatgc gaattcctga tatatttgtg
    16261 tagttgttgt taacttttaa agtaaactcc tcattttttg aatatggaat aatacgcatt
    16321 ttgtcaatgt tgtctctatt gtagtttggg aaaacttctt ccaacaaatc ggtcagaatg
    16381 gtatcacgcc taaaattttg aagaccatat tctgcaattt ctttttgatt tcccttgcga
    16441 acaatttcag ctgcttttgc ttcggttaac cctgcttcca attgcttatc cgtaagaaaa
    16501 ccttctttca tcacaatttt ctttttggct gttttcaaaa aatgaaacaa ggtgtcaaag
    16561 ctcgctgtgt aacgtttgtt ttgatcttta aattctattt gcgctttacg aatatcaacc
    16621 aaacactgaa taacatcttt ttctctctga ttcttaatcc tttcgaaacg aataggagta
    16681 atgatactag ctacacaaag atacgaaagt aaaacaatag aaagagaaag caaaatttta
    16741 atgactgttt tcataattga aagattcaat tgttttttaa aatacttatt ttatggagaa
    16801 taacgctacg ctattaactt tttgcaaatt tataaaagaa aattcaatac tccatctttc
    16861 tttcaatatt ttttacctta tttttaatat cgcattcaag tgcttactgg tagaacgtat
    16921 tttgagtaac aattgatccc cttccataca gaattaactt gctcattttt catacacgca
    16981 aaaataacaa tttgttttta aacctttggc tttttttcgg attaacaaaa catgtaatcg
    17041 ttatatcgga gttacgttaa attaataatg catttaattt caaattcaac acactaatat
    17101 aaaaatttcg atgaaaatgg aaatttttat aaacattaat aaacacacat ccaaacatta
    17161 aaaaatatta tttatatttg gaatcaaatt gttggaaatt tctttcattt tgaaacctta
    17221 agtctttttg caacatgaag aaaatgcttt gtctgttgtt gattgcaagc acagtatccc
    17281 aaatttttcc tcaaatttct tcaattattc cggtttcttc ttctattgcc ggaacctccg
    17341 tagccaatac cggcctgaga tccgaatttc aaaatccggc catgttgggt tatgccaaga
    17401 acagtgaaat tggtatgcaa catgaaaacc gatacgcact ttccgagttg tcaaccaaca
    17461 gctttcaagc tcttttgagt accagtgcaa ttaacgcaga tttgtcgttt tcttattttg
    17521 gttattcatt gtaccacgaa atgatggccg gtttgggttt cggaagaaac ttttccgaca
    17581 aatttgcatt gggtgtgcgg ttcaattatt acatggcata cttttctgca tccaattcgt
    17641 atcgtggagc cttgttgccc caaattggat tgtcataccg gttttcgtcg ttattcagtt
    17701 tgggcttcag tacttttaat ccctttcaga caaacataaa aaccgatttt acacaaaaaa
    17761 gaattccttc cattttcagt ttggggacgg aatattattt ttctcccgat ctggtgtggc
    17821 tggctcaaat cgacagggaa atcagcagca attatcgttt ggcaacaggt ttcgaatacc
    17881 aaatgcttca gcaagtttct ctaaagttgg gcgcttacga tgccggttat ctggttccct
    17941 gcggaggatg tggactgaag tggaaccgtt ttttgctgga tattaattgc gaattgcatc
    18001 ctttattggg attgaactcc tatgccggac ttcgatatat attttctcgt taatttttta
    18061 gtttaacgtc cctatcatga agcgcattgt catattagta tgctgtttgc ttttccttct
    18121 cttaaatgca gcctatgcac aagatgcgtc cacaactatc gatcagacaa tagccgatat
    18181 ttttgaacag tggtctgcag aatcggaaga agttcccgat tatcaaacat tttacgagga
    18241 attggaattt cttgccgaaa atcccattaa tctgaatcat acgtcgagag aagaattaag
    18301 taaaatgctt tttctgtccg atgtgcaagt cgagaacatt cttgcttatc tttatcagta
    18361 tggaccaatg gaaaatattt acgaactaca gctcatagat gggctggata tgacggacat
    18421 cagacgaatg ttgccttttg taatggtgaa aaaagaaaaa gcggtttctg aaaaaatcta
    18481 tcgttatgac gttttgaatt acgggaaaaa cgagttgtac atgatgttca acagagatgc
    18541 ggaaaccaga caaggctatc aggcatcctt ttcatcaatt ggagattcaa caaatcaggg
    18601 gaaaaaatat gcaggttcgc ccttgtatca ttctgccaaa tatcgttttc attttaagga
    18661 tagaattttg gccggaatga ctttcgagaa agatgccggt gaatcttttt ttggttctgc
    18721 ccacaaaggt tacgattcct attcgggtta tcttcaaata aatcgtttgg gaattttcaa
    18781 aaccattgtt ttgggtgatt tcagagccaa ttttggtcaa ggtttggtta tgaagtccga
    18841 tttcagtatg ggaaaatcct cctatgtcct taatgttgta tcgcgcaatg aagggctgaa
    18901 aaaattcagt tctaccgacg aatacaattt tttcagagga ggcggagcca caattcggct
    18961 acccagaaat tgggaaactt ccgtttttta ttccaacaaa atgatagatg ccgatactgt
    19021 cgggggcgtc ttttcgagca tttataaaac aggttatcat cgtaccgaaa acgaattgaa
    19081 taaaaaacat accatttgcc aacaggtagc aggcggaaat atttcatggc ggttgctttg
    19141 gctgcaaacc ggaataactt ttgttcacac caattttgat caggtacttg tgcctgaaaa
    19201 aaccacctac aatcgttttt atttttctgg gaaagcccaa agcacagccg gatggaatta
    19261 tcgtgtccat tggcagaaac tcaatctttt tggcgaagca gcagttacag gcaactttca
    19321 tcctgccttg ttgagtggat tcacgctgtc gcctctttca ctagtcaatc tggtagctgt
    19381 ttaccgatat tattcacctg aatacgatac tttttatgct ttggcgcttt ccgaaacttc
    19441 acgcacaaac aatgaaagtg gtttgtatct tggagccgaa ataaatccgg caaaaaaatg
    19501 gaaaatttct gcttatgccg acagttatcg gttcccttgg ccaaaatatg gaatcgatgc
    19561 tccatcggcg ggaaaagatt atctttttca gaccgatttt tctgcccatc ggcgtttatc
    19621 catgttttgg cgattcagac acagcaacaa accggccaat atttcacagg agaattcagt
    19681 tatgcctgtt gtaaaagcgg tcaagaaaaa ttcattgcgt tatcagttga tgtattcgca
    19741 gggaaatttc agtttcagaa ctgcggcaga aggaaacatg gtacagaagg gagaaaatgc
    19801 cgattatact tacggattga ctgcctatca ggatttgact tatgagtttt cttccttacc
    19861 gttgaagctt gatttcagat accagttttt cgatgcggtt gcctacgaaa accgcttgta
    19921 tgcctatgaa aaagatgttt tgtatgcttt ttccatcccc atgtattatg gaataggcag
    19981 tcgttattat cttaatctga aatatgagct gaaccggcag tttactttat ggttcaaaat
    20041 agctcaaacc gtatatgccg acgatcgtga agaaatcagt agcggaaacg agaaaatttt
    20101 aggcaacaaa ataacggatg tgaaatttct gctgcgtttc aatttttaaa aaacaggatt
    20161 ttttctgcta aatatcgttt cagggttgat acgggctgat acattttata actatcattt
    20221 tttatgcttg ctttacaata atagtaatgc atgaacttta taaacttttg tggctattaa
    20281 ctatatattc aaattttatt gcaataatca ataaaaaata ctgtactctt ttaaaaatat
    20341 catttcttta ctattttcaa gctcaaaatt gccaatttcc tattaaattg taggcaatat
    20401 catttctatt tcccttattc cattttctac ttcttgtata tttactttca ctgtagagtc
    20461 tggtaaaaga gcttcaattt tttcaggcca ttcgatgaaa caaatttttc cgctataaaa
    20521 ataatcttca gctcctaagt cgatggcttc ctgtaactta ttaatacggt agcaatcaaa
    20581 atggtacaaa actcgtccat ctgctgcttc atattcatta ataatggaaa aagtaggact
    20641 attcactgta tctttcactc caagaatttc acaaacagct ttaataaagg tcgtcttacc
    20701 ggctcccata cttccataaa aagcaaaaac cgagtatcta tcaaataaat taataaattt
    20761 tcgcgctggc tgctgaatat tattcaagtc aaatatttct gtcatgttca atttttaatc
    20821 tttaaagtta ttacaaataa aaatttattg accaaaatac actcattttg aaatttgcga
    20881 ttctggagca ctcaaatctt tgtgaaaata cccataaact attgatgctc tgtgatttcc
    20941 gacaactttt tgaatatctt ccagatttgc attctggagt ctttttatac ttttaaaatg
    21001 atttatcagt tctcttttgg tcttatcccc tattccttta atattatcta gttcagatgt
    21061 ggtttgattt ttacttcttt tctcgcgatg aaatttaatg gcaaagcgat gcacttcttc
    21121 ttgcattgta gccaacaatt tgaaaagagg ttcattgggt gtcatgccaa tattttttgg
    21181 aggaaagccg aaaagaattt cgcgagtatg gtgtttactg tctttggcaa gtccaataat
    21241 gggaattctt aaattaagct catcttcgac aacttccctg atcacttcca tttgtccaat
    21301 tccaccatca gctatgatga gatcgggcaa ttctccgttt tctgctatca tccgagagta
    21361 acgtcttttt actacttctt tcatggaagc atagtcatcg ggacctacaa ctgtcttaat
    21421 gttgtatttt cgataatctt tttttgaagg tctccccttt ttgaaaacca cgcaggctgc
    21481 tactgcatct ttcccctgaa tattggaatt atcaaaacat tcaatagtta ttggtaattt
    21541 ctcaagatgc aatttctcct gaatttcttt taatagctgc atacttcttt gatcaggatt
    21601 taatttttca gcttgcttaa gtttctcaaa cttatattgc agcacatttt gctgagccaa
    21661 atccaagagt tttttccggt ctcctctttg cggaataaca atgctaacgc cattcggctt
    21721 ataattgatt tcaaaaggta cgacaatttc tttggagtta ctttttaatc tctctcttaa
    21781 ttctactatg gccatagcaa gtaaatcttc cttttcctcg tcaacatgtt tttggtactc
    21841 gatggtatat ccctgaatta ttgaaccttt tgaaattctc aagatattaa tataagcagc
    21901 attttctgtt tcttcataac caaacacatc tgtgttttct accactgaat tggcaatgat
    21961 cgactttgat ttgtaatttt caatcaaatc atatttttgt ttgatttgat atgcttcctc
    22021 aaatttgtaa gcagcagaaa gcttttgcat ttcttcccaa agcattttgc ttatttcgtt
    22081 cgcattaccc tcaataattt gcttaatttc gtttatgtat ttccgataaa tttcagcaga
    22141 ttcttttcct tcacacggcc cattacactt atggatatga tattgaaggc aaactttaaa
    22201 ttttccagct ttgatgtttt catcggtcaa ctgaagtttg caagttcgga tgggataaag
    22261 atcatgtatt atctctaaaa gagaattgat cgtgtaactc gaactatagg gtccaaaata
    22321 taaggaacca tttttaataa tatttcttgt tttgaatact ctgggaaatt cttcattcgt
    22381 tatacaaatg cttggatagg tttttccatc cttcaataaa acattgtaac gcggttgaaa
    22441 tttttttatt aaattgtttt ccaacaacaa ggcatcttct tctgtgtcaa ccacgatata
    22501 tttaagagat gagatatttc ttaccaatac tgccgtttta cgtgattcgt gcgttttggt
    22561 aaaataggag gaaacacgct tctttaaatt tttagccttc ccaacataaa taatctcctc
    22621 cttttcattg aagtattggt aaacacccgg cttttcgggg agagcatcaa tttgctgctt
    22681 taattgagga tgcaaattca taatactttt gaatgtttca cgtgaaacaa agttagaaaa
    22741 aattctttaa tttcccgatt gttccacgtg gaactataaa attatcgtcc cattagaact
    22801 aaaagtatat taatgtcact tggagaaatt cctggtatcc tactagcttg accaatagtt
    22861 tcaggatcaa tttttgacaa tttttgtcgt gcctccgttg ataaggatga aatagtatga
    22921 taatcaaatt ttcctttaat attgatgtgc tctaacctgt gaaacttttc tgcaatagat
    22981 ttttcgcgat ctatatatcc tttatatttc aaagaaattt ctgctgcttc gactatttct
    23041 tcaactctat ctcctattga ttgaattttt gattttaaag caggtacaat ttcagataat
    23101 tccataatag aaatctgagg acgaagaatc aaatcaacaa gtttacaacc atgagttaac
    23161 tctgaagaat tttttgacac taaaaaacca ttcacttgtg caggtttgat tgagaaattt
    23221 tcaacaaaag tttttaaaga ttctatagcc tcgtatttga aagaatattg atttattcga
    23281 ttctttgtaa ctaatccaat ttcataaccc ttgggtgtta accgtaagtc tgcatcatct
    23341 tgacgtaaaa ggatacgata ttcagcacgt gaagtaaaca tacgataagg ttcgtcaact
    23401 cccttagtaa ccaaatcgtc aattaaaact ccaatatagg cttcatcacg atccaataaa
    23461 aattccttcc ctccatgaca atttatatgc gcattgatac ccgcaattaa tccttgtccg
    23521 gcagcttctt cgtaaccggt agttccattg acctgtcccg caaaaaataa atttttaatg
    23581 cgtttggttt cgagtgtatg ctttaattgc gtaggatcaa aaaaatcgta ttcaattgca
    23641 tagccgggac gataaagttc ggctttttca agacccggaa tcgtatgtat agcttgaatt
    23701 tgaacttgaa caggaagaga agaagaaaaa ccgtttaaat agtactcttg tgaatcaact
    23761 ccctctggtt ctaaaaacag ctgatgagaa gatttatcgg caaaagtaac aattttagtt
    23821 tcaatacttg gacaatatct cggaccaata ctttttattt gaccgttata aagtggagat
    23881 tcgggcaacc cttgtcgtaa aatgtcatga gttaattcat tcgtataagt tatccaacaa
    23941 ctcatttgtt tcaattctcg tgttacttca gtgagaaaag aaaatttatg aaaatcgttt
    24001 tctcccggtt gttcctttag cacagaaaaa tcgattgttc ttccatcgat acgtgctgga
    24061 gttccagttt tcattcggcc ggtagtgaaa cccaatgatt ttaattgctc tgtaatacca
    24121 aaagaagcag gctcagaaat tctacccccg attagttgat ttttaccgat atgaataagg
    24181 ccgctcaaaa atgtaccggc tgttaataca acacatttag ctgagatttc tattcccaaa
    24241 gcagttaaaa cacctgtaac ttgcccattt tcgataagaa tttgctttac tgtatcttgc
    24301 caaatataaa gattaggggt attggttagc gtctgaatcc actgaagaat aaattgatgt
    24361 ctatcacttt gggagcgagg actccacatt gccggacctt tagaacgatt aagcatacga
    24421 aattgaatgg cagagcgatc ggtaacaact cccatttgac cgccaagtgc gtctatctct
    24481 ctaacaatct gaccttttgc tatgccgcct actgcaggat tacaactcat ctgaccaatt
    24541 ttattcatat ccatagtgat gagcagcgtt ttagatccca tattggcagc ggcacaagca
    24601 gcttcgtttc ctgcatgacc gccaccaatt acaattatat catatttaaa atccatatcg
    24661 agtaattaaa ataatatccg agcaaaaaat aacaaaagaa taaagtatta gttcaaaaaa
    24721 taggataaaa aaaatatcag gcaaataaag ctttgccaat tatggtggaa atttgctcca
    24781 gataataaat atcagcttca tcaagaacac caaatttttc gctatctata tcaagaacgc
    24841 caaccacttc tgaagaagaa ttgtacaaag gaataactat ttcggaaacc gaaatactgc
    24901 tacacgctat atgtccagaa aaatcattaa cgtcaggaac aagaagactg cgtttttcct
    24961 tccatgctgt tccgcaaaca cctttaccaa aggcaattcg agtacaggca acaggacctt
    25021 gaaacggccc aagcacaaga aaatcttcct taaccaaata aaagccaacc caccaccatt
    25081 taaaagtctg atgaaaaacg gccgataaat tagcaagatt tgctacaata tccggttcat
    25141 cagctataag agcaggaatc tgttgtaata aaatctcata tttggatttt ttatccagag
    25201 aagaactaat aaagaaattt tcagacatca cgaaaaattt ttttgcaaaa ttacgctaaa
    25261 aaaatcattt ttaaatggat taaataggaa tcaaacaaac aaagaaaaag tcaatttaac
    25321 attcatatct aaaaatttcg aaataatttt cttatctaaa attttatcaa ttactttgct
    25381 acaaatagaa gagaacgttt tgagatataa aaaaattatt attaaaatgt aaacaaaaaa
    25441 atcatgagaa aattagagtt taaatttttc ccttttgtac tactgatttg tctgaataca
    25501 tcaatgtttg cacaacaagt gcagttccaa cctttgccga tagacccaaa agtcaaatat
    25561 ggaaaattgg aaaacggatt aacttattac attcgttcaa atcaacaacc aaaaaacaga
    25621 gcagattttt atatcgttca aaatgtgggt gcaatactcg aaaacgatga tcaaaatgga
    25681 ttagcgcact ttttggaaca catggcattt aatggcacaa aaaatttccc tgggaaaaaa
    25741 attattgaat acttcgaaag tgtaggagta aaatttggag ctaacatcaa tgcctatacg
    25801 tctcttgatg aaacggttta tcatttatcg aatgttccaa ccaacaggga aagtataata
    25861 gacagtgctt tacttgtgtt gcacgattgg tcaaatgcaa tttcgctcga agaaagtgaa
    25921 attgaagcgg aaagaggagt tattctcgag gagtggagac agggagcaag tgcggatcga
    25981 agaatgtgga aagaatcaaa caaacaaaaa ttccccggct cacaatatgc caaacgtgat
    26041 gtgattggag atactgcagt tataaaacat tttacgccgg aagcattacg ggattattat
    26101 aaaaaatggt atcgacccga ccagcaggca atcattgtgg taggagacgt agatgtaaac
    26161 aaaattgaag aaaaaattat cgaattgttt tccaatatca aaaaaccgga aaacgcacct
    26221 gaaagaccaa tttatccaat atacgacaac aaggaaccaa taatctctat agttactgac
    26281 ccggaagcca gaatgacaag agtggaatta atttataaac acgacaagtt accggcagaa
    26341 attaaactgt ccataaacgg ctatctggtg aatgtgataa attcgttaat ttcaaatatg
    26401 atgcaaaaca gatttgaaga aataaccatg cagcccgatg caccttttgt agccggatat
    26461 gcaacgtatg gagaactggt caaatcaaaa gatgcattct atctggtagt agtaccaaaa
    26521 gaaggctcag aaactgaagg aattaaaagc gttttaattg aagcagaaaa agttaaacgt
    26581 tttggattca atcattcgga attggaaaga gcaaaagccg atttggcaaa aatgatagaa
    26641 aaacaatata atgaaaaaga taatcagaaa aatgaaagtt atgtgagaga atatatggaa
    26701 cattttctga atcaagatcc cataccgggt atagaatggg aatacgaaac aattaaaatg
    26761 attttgccac aaataaatgc cgatatggtg aatcaacttg caaaatctta cgtaaccgac
    26821 acaaatatga ttgtttcgat aatggcgcca caaaaaccta cggtagtcct tccaagtaaa
    26881 gaagaaatca ggcaaataat tgaagaagtc aaaaaaacgg agctaactgc caaattggac
    26941 gaaaaaatag ataaaccttt aattgaaaaa atgccaaaac ccggtaaaat aaaaaaagta
    27001 tcaaaaaatg atattcttgg aacaacagaa tggatattga gcaatggtgc aaaagttata
    27061 ttaaaaccaa ccacattcaa aaaagatgaa atattgttca atgcctacag cataggaggc
    27121 ctttctaaag ttgaaaatat tgaagattta ccttcggctg aatttgcaac agaagttgta
    27181 agcaacaacg gcttgtccga tttcaatcaa atagaattga aaaaattact ttcgggaaaa
    27241 attgcttcgg tcagtcccta tatcgacaat tatgaagaag gcttctcagg aaattcttcc
    27301 gtatccgatt ttgaaacaat gatacaactt ctatatttac attttactgc tttaaggaaa
    27361 gatgataacg cctttacggc tctgatgaac aattataaag ccttcctggc aaacagcgag
    27421 aataatcccg acaaggcttt ttccgattct gttaaattaa tgctttccaa ccacagtccc
    27481 cgtacggtgc ttttaaatat gaaaacattg gaaaaagtca accaggataa agcattggaa
    27541 atatttgctg agcgattttc tgacccggcc gactttacat ttgtgttcac agggaatgtt
    27601 gatccaaatg ataaaacggt agtcaatgca attaatactt tcattggcgg aattaaatcg
    27661 aaaggcaaaa aagaatcgtt tactgatgat aatgtaagac ttccagtggg aagagccaac
    27721 aaccattttt caagagaaat gcaagtcaaa aaggtttcaa actgcattgt ttacagcgga
    27781 aatttgaatt ttaacatgaa taatcgaaca aacatgaatg ctataggaaa tattttaaat
    27841 attcgctatc ttgaaagtgt tcgagaaaaa gaaggtggat catatggcgt aggcgttgct
    27901 gccaatttga gaaatcgacc gattgaacag gcaatgctgt tcattcaatt cgatacgaac
    27961 attgaaaaac aaaacaaatt gatggatatc atacattccg aaatcgacac cataatcagc
    28021 aaagggccaa aagcagaaga cttacaaaag gttaaagaaa caatgatgaa gaaatacgag
    28081 gaagatctgc aagaaaataa atggtggcaa agtgcaataa aaaattatta tttcaataaa
    28141 ttgaatgtag ttaacgatta tcttccatcg gttcaagcac tgacaagtga aagtattcaa
    28201 aaaactctaa aaactattgt ggatcaggga aatattatgg aaatagttat gaaaccgaag
    28261 gaataaaatc aattgcagct caataaaaaa atttctctgt aagaattcag taaaaaaaaa
    28321 tcttacagag aaatttttta tttatacaac tgctagaatt accaaatgca ggcgcgcttt
    28381 tctacaggca aatacaattt atctccttcc ttaattccaa aagcatcata ccatgcctga
    28441 atatgaggca aagccccatt taccctccaa cgacccaaag catgcggatc agactttgtc
    28501 aaaacaagaa tttcttcggg acgtatgttg caagcccaaa cattagcata agacaaaaag
    28561 aaacgctgtt cgggtgtgaa accatccaaa atttttgatg gctggccttt tgttgaattt
    28621 ttataagcct gaaatgaaac ttgtaaacct ccatgatcag caatgttctc tcctaaagta
    28681 aattttccat tagcatggac accgggagca acttcaatgg aatcgaaata atcggccaaa
    28741 acttttgctc gggcatcaaa ctgcttctta tcttcttcag tccaccagtt ttttaaattt
    28801 ccatccttat cgaactgaca accctgatca tcaaaaccgt gcgtcatttc atgacctatt
    28861 acaacgccaa tggctccata attaacagca tcatcggcat gtacatcaaa aaatggaggt
    28921 tgcaaaatac cggccgggaa acaaatttcg tttgtagaag ggttgtaata agcattaaca
    28981 gtttgaggag tcatgagcca ttcggactta tcaacagggt taccggctct tgcaaacttg
    29041 taagcatact caaagtgatg agctcgttta acattttcat aataagaaac atttttagaa
    29101 atttgcagcg catcataact cctccactta tcgggataac caatttttac ataaaaagtt
    29161 cccaattttt ccaaagcttt tgcttttgtc gaatcgccca tccatgttaa attttgaata
    29221 cgctgaccca atgattcctg aagattgtgc acaagcttta acatactttc tttagcttcg
    29281 ggaggaaaat atttctttac gtacatttga ccgactgctt cgcccaaaaa attgtcaata
    29341 cttctcaccg atcgtttcca acgaggcgaa ggttcttttt tcccagacaa agtttttccg
    29401 taaaactcaa aattctgatt gtaaatactg tcgcttaaat aagcagcagc attatcaata
    29461 accttccatt gaagataagc gatctgttga tctaaaggaa cggtagtaag aatattcaag
    29521 acttcagtca aaaattcttt ttgagaaaca tttactgaat caacagcagg aattcccaaa
    29581 gtgtgaagaa attcattcca agaaatagta gaaaattctt tacttaaatc ctgaatggac
    29641 attttatgat aattggcata aggatcgcga agttttacac gatcataaga cgctttggct
    29701 aaacgagttt caatatacaa aacagcatcg cgatttttct gagcatcggc agaattgaag
    29761 ccaaataact caaacatttt agctacatgt tgtttatact tttcaagaat ctctttggaa
    29821 tgcgtgtcct gatccaagta ataatctctt tctcccaaac ttataccgct ttgataggtt
    29881 tgtgtcaaat ataccgaact attcattgga tcagcatcaa cgtaaaccga aaaataaacg
    29941 tcgttgccat tcagcagtag ctccggaaga attttaatca actcggtgtt ggtttttaca
    30001 ccagccaatt ttttcaattc agtttgcacc ggttgatagc cttccctatt taaacggtcg
    30061 ctatccattg ccaattggta caaatcacca attttttgct ccacagtacc ctcaacatgc
    30121 gatttagaag caatttcttc tatcaattca cgaagctgag aagtattttc ctccgccagc
    30181 ttgtcaaaag ttccgtaacg tgcatattca ccttttaaag gatttttttt catccatccg
    30241 ccacaggcaa attgataaaa atcatcacca ggacgagcag tagtgtccaa atttgtcaag
    30301 tcaatgccag aaattttttt ttctgattta catccattca tcataagcat cataaaaatt
    30361 aaaaatggta tccccaacga gagaaaacca aacatacgtg atttaataat aaaattcata
    30421 aacagtagaa tttagaaaat ataattattc aaaaaaacta attgcaaaaa gtttcatgta
    30481 cagaatcgat aatatcgaaa agttcacgca atgtatcggc acgaagcatg gcaatacgcg
    30541 tacttttaaa atttggaata ttcttaaaaa tagaagttgc tgccaaatgt cgacgagaat
    30601 gaacaattcc ctttaaatgt atatcataaa cattttctat aggtgtttgt tcatcaatat
    30661 ccaacctgcg aacggcagat aaaatatagt tttttaaaat agctttctta tcttcaaaag
    30721 aaagaggcga atattcaaag tcgtcatcaa ggacatgctt catttcagaa aaaatccatg
    30781 gctgaccaat agcagctcgt ccaatcataa ttgcatcaac gccataacga ttgaaaaaat
    30841 cttttgcctg ctctggtctg gtaatatcac catttccaat aataggaatt ttaattcttg
    30901 aattgttttt gacttcacca atcagcgtcc agtctgcctc gcctttgtac atttgttgac
    30961 gcgttcgacc atgaatagtg agcgcctgaa taccgcaatc ttgcaacatt tcagccaatt
    31021 caactataat cttactgctg tcatcccaac ccagacgcgt ttttaccgta acgggaacgt
    31081 ttacagcttt caccacaaat tgagtaattt tcaacattct tggaatatct ttcaacaacc
    31141 cagctcctgc cccttttccg gcgacttttt ttaccggaca tccaaaatta atgtctataa
    31201 tatcggggtg aatcatttca actttctgag ccgattcagc cataacctcc gggtcggtac
    31261 cgtaaatttg aattgctaca ggtctttcct cttctaacac attgattttt tgtaaagttc
    31321 gaggaatatt acgattcaaa gcctcagacg aaacaaattc agaataaacc atgtcggctc
    31381 caaaatgacg gcataataag cgaaaatcag catccgtaac atcttccatc ggagccaaaa
    31441 acaaaggtcg tgaaggaaat tctatgtttg aaattttcat ttttatgcaa aaaagagtac
    31501 aaatttagaa aaaaaattgg attgatgatt tctaaaatgg aattaaaaaa cccatacgag
    31561 tgaaaaaagt tgctccataa atagaaataa accaagtaaa agattgagaa cgtgtagtga
    31621 ggagttgaat agaaaaatta aaataaaagg gttaattttg cagtaagaaa ttagaaaaaa
    31681 agcaagattc aggagaaaat caaacaatgg aaaattatat aaacaaaacc gaaacttatc
    31741 caaccgaacg cagcaaaata gaaattatgg caccggcagg atcatgggaa agtttggctg
    31801 cagccataaa agctggagca gactcagtat atttcggtat tggaaaatta aatatgcgta
    31861 gcaaatcgag cagtaatttt agcgttgacg atttgaaaaa aattgttgaa atatgcacag
    31921 aaaacaacgt gaaaagttac ttgaccgtga atacaatttt atacgacaat gatttgattt
    31981 cgatgcgcga aattatcgaa acagcaaaag aagccggtat ttcagccatt attgcctcag
    32041 acgttgccgc catgatgtat gccaatagcc tgaacgttga agtgcatttg tctacccaat
    32101 tgaatatttc caacatcgaa gcattgaaat tttatgcaca gtatgcagat gttgtagtgt
    32161 tagcaagaga attaaactta aaacaggttt cggaaatttt cagcgctatt caaaccaaaa
    32221 atatttgcgg tagaaacgga aaactgattc ggattgaaat gttttgtcat ggagcgcttt
    32281 gcatggccgt atcaggaaaa tgctatctca gccttcacga aaaaaatcta tctgctaatc
    32341 gcggcgaatg caatcagatt tgtcgaagag gctatctggt aaaagacaaa gattcggaaa
    32401 tagagctaga aatcgacaat gaatacatca tgtcgccaaa agatttgaaa acaatagaat
    32461 ttgttgacga aatgctgaaa gccggagtca gtgtatttaa aatagaagga cgtgctcgcg
    32521 gagccgaata tgtgaaaaca gtcgtttcat gttataaaga agcaatcaat gcctgctatg
    32581 aaggaacatt cagcaaagaa aagataaacg actggaaccg cagattaagt aaagttttta
    32641 acagaggatt ctggaatgga tattatttag gacaaagact tggcgaatgg agtaaaaact
    32701 atggttctgc agcaacccac aaaaaaattt acatcggaaa agttaccaat tatttcaata
    32761 aaattggcgt agcagaattt ctgttggaag ctcaaagttt atcggtaggc gacgaaatat
    32821 tgattactgg cgaaacaacg ggtgcatacg aagacattgt caacgaaata agagtagatt
    32881 tgcaacccgt tgagaaagtg gaaaaaggaa cctacttctc catgaaaacc aatgaattgg
    32941 ttcggcggaa cgataaactt ttcaaaattg tgcctaccga acacggtaaa caagaaggaa
    33001 aataaatatt tttttggtaa ataaaaaaat atacttttca gaaaatttct tctcttggaa
    33061 atttatcttc caacttgcta aaaaggaatg aaataaccac accaacagca agaaaaccaa
    33121 tacacaaaag aaaagcaccc acaaaactta gtcctgcaac cgacaattgt tcagaccgaa
    33181 aagcgggaaa aacaaccgtc ggaaaaattg ccaatgatga ccctactacc attcccaaaa
    33241 taaaatgata catttcggga taaaactttc tgaacaaata agctgccagc tttgcaaaaa
    33301 gtaaaacaca aaccgcaaaa ccaacaagaa gaggaataat cacggcaaaa tcaaaatcct
    33361 taattccggt tgccatttta tcgtataatc cgaaataaat caaaaagttg gaaggactca
    33421 taccgggaac aatcaccccc aaaccaatca gcgcaccgga acccaaccaa accggaatat
    33481 tgggagcaac ttccgtcaac tgctgaccac ccagcaacat taacccaaaa ataaaaaacg
    33541 tagaaaaaat aaaaatgata atatcttttg ttcttctccc ctgttttcct gctgttctat
    33601 aaagcgatgg aaaagttccg cctacaaatc ccacaaaaag gcaaataaat tgagcggcat
    33661 aattaccaaa agccttctcc accaaaatag aaaaaagcac gattccgatt gccccgccaa
    33721 ttactaccgg aataaaataa aaaacattct gaacgaaatc ctttcgcaaa tcggacaaaa
    33781 atttgataat tctgtcataa attcccaaaa ttactgcaag cactccgccc gacaaaccgg
    33841 gtaaaataaa accaataccc acaattatac cctttatcaa tctaataaac cagtcaacaa
    33901 caacatttga ttgagtattt ttcgagccgg aagtattcac tttgtcagtt acttgagtca
    33961 taaaaaaatt tttaaaaatt tcgcttgcaa agttacactt ttttcgttca attggagaaa
    34021 agaaaatttt ggatagaaaa aaacagaaat gaaatgttag atgtaaagat ataaaatata
    34081 ctttaacaaa tgctttatca aaaaaacatg gaaataccca tcaaaaaaat aaaaacaata
    34141 aaaaatggcg tgtaaaactt ttccaatttt ttttcgtcaa ttttctttcc caaaattggg
    34201 cccaaaatag ctccggtaat tgttcccgca gccaataatc ctaccaaata ccaattgaga
    34261 tgaccaaagg cggtatgtgt tatgactcct gaaagagcaa taaaaaaaag taccataact
    34321 gacgttccca ccacctgcac ggaactcaac ttcatattgt aaagtccggc taaaatgggt
    34381 ggagttccac ttactcccag caaaccggac ataattcctc caaaaaaacc aaaacttaaa
    34441 cccttaaaat ttttgaaact aaaaattttt ttcttttttg ctatgtttac agttttatct
    34501 tcttctacta aaataatatt tcccttattg aaagattttt ttgaacgaaa atttttaaaa
    34561 atcatttgca cagccataaa tatcatgaac aaaccaaata caatttttaa aacattcaag
    34621 gataaaagtc ccaataaaaa agagcccgct ataactccaa taattccacc ggctgaaaga
    34681 atcaatccta cgtttatatt tacatttcca gccttaaaat ggcttataga ggctgcaaaa
    34741 gtagttggta tgatggtggc aagtgaaaca gaaactgcct gattcatagg aatggaaaat
    34801 aatccagtca aaatggcaat ataaaaaatt cctcctccgc ctcccatcaa agaaattagt
    34861 attccaatca ataaaccggc taatggcaaa aaataaatag gtaaaatcat tattaaaaat
    34921 tagttataca attttttttc aacttaattc caacattcgc aaaataggtt ttacagcttt
    34981 ttgtcggatt tcctcctcaa ccaaaatctc tggactttca tttaaaagac attgatatac
    35041 cttttcaagc gtatttaaac gcatgaaatt gcattcgttg cacgcacacg tgctgtcgtt
    35101 tggtggaact ggaataaatt gcttgtcggg atttttcttt tgcatttcgt gcaaaatacc
    35161 cgattctgtt gcaaccaaaa aaaatttttt atcggatgat gtagcaaaat tcaataaagc
    35221 ctgcgtgctg ccaataaaat cggccagcat taaaagtggt ttcgtacatt cgggatgagc
    35281 caaaaccaat gcttcgggat atttcttttt caattccaca agtttttgta ccgaaaactg
    35341 ctcgtgtaca tgacaagctc cgttccacaa aagcatttgt cggccggtta tgctgttgat
    35401 gtaatttccc aaatttttat ctggtgcaaa gataattttc tcttcttttg gaaaagattc
    35461 cacaattttt ttggcgttgg ttgaagttac tacaacatca gttaaagctt taacagcagc
    35521 cgatgtattg acataagaaa taaccgtata atcgggatga ctttttacaa actcttcaaa
    35581 ggcatctgcc ggacaactgt cagcaagcga acaaccggca ttcatatcag gtatcagcac
    35641 ttttttttgc ggattaagaa tttttgctgt ttctcccata aaatgaaccc cacaaagaac
    35701 aataatatcg gcatccgttt gcgctgccca ctgtgccaat gccaaactgt ctccaacatg
    35761 gtcggcaata tcctgaatat ctggctcttg ataaaaatga gccataatca cagcattttt
    35821 atcttttcga agtttattta ttttttctat gagctctttt ttttccatag ctttgttgtt
    35881 atcaaatata tatcatatta atattttatt caattaaata aataatatga atatgataat
    35941 agggtgttaa tttggttaat atttattaga aaaaattctt gcttaattaa ttattaaatt
    36001 ttatagtata tttgtgcact ggtgcgggta atcaaatagt ttgaaaaaca gaattttata
    36061 aatgttatca acaggttgtt tataactaca aagttaataa gtttttgaat tgtttcggtg
    36121 aataaatcta ttaaatatgg ttgaaaaatt atatgaaaat atttaactct aaattttcaa
    36181 aatccaaaac aattgatatg gaaaattgtt tttggtcttt caataaaaat tttcaaatga
    36241 ttataaatta gttttcaaca acatttcagc gtagaatgtt gataaataaa aattctatga
    36301 actgtaaaga aataattgaa aatgaatata ttgctttaaa aatgaattaa aacttttgag
    36361 tagtgaaaat tttttcaact tcagtagttc gtcgtttgga cgaatatacc ataaaaaaag
    36421 agcccatcgc atccattgat ttaatggaaa gagcagctgt tgggctttgt aaaaagtttt
    36481 tagaaatatt tccttctccc ctacccgttt gtatttttgc agggcccgga aacaatggag
    36541 gtgatgcgct ggctttagca agaatgctgc ttcaaaaaaa ttattatgtt actgtatatt
    36601 tgattcatac aggaaaactt tctgcagatt gtgaagccaa taaaatcaga ctgatagaaa
    36661 aatttcccgg ctgtttgcat gatttttcta ctgactttta tcctgtgaat ttttcggaaa
    36721 ataccgtcat aatagatggc ctttttggta cgggtttgtc ccgaccattg agcggaattt
    36781 attcggaagc ggttgggtgg ataaataatt ctggaaaaac cgttgtatcc atagatattc
    36841 cttccggatt ggagggtgaa gaaaataaag atttatcaca acccatagta aaagccgatt
    36901 atacattttc gttgcaattt ccaaagcttt cttttttctt tgcagaaaac gaatcttttg
    36961 tggggaaatg ggaaataata gaaattgata ttcatcctga agccattaga cgagaaccaa
    37021 ccgattttta ttttttagaa aaaaaagatg taattccgtt tttaccggaa agaaaaaaat
    37081 tttcccacaa aggtacttac ggacatgcat tgatagtggc tggaagtgtg gggatggctg
    37141 gagctgccgt tttatcggct caagctgcca ttagaagtgg aacgggattg gtaagcgtgc
    37201 attcggctgc aacaaacaga attattcttc agacaaaagt tccggaagct ttgtttgaaa
    37261 gcgataagga agaaaatttt atttcagaat taaaaaactt ggagaaatac aacactttgg
    37321 ccattggtcc aggtattggc actcaagaaa aaacagccaa ttttttgttc caaattcttc
    37381 cttcaatcaa acaatcgatg gttctggatg ctgatgcctt gaatattatg agcagaaaaa
    37441 aagaattgtt ggaaaaactt tctcacaata cccttcttac gccacatccc aaagaatttg
    37501 aaagactttt tggtgcggat aaaaattctt atgatcgttt attgaaaatg cagcaactgg
    37561 caaaaaattt gcatctggtc attatcctga aaggagccta tacgcgaatt gcctttcctg
    37621 acggcagtat ctattttaac agcaccggca atccaggaat ggctacagga ggaacaggtg
    37681 acgttttgac aggaatactg gcaggtttac tcgctcaaaa cctttctccc gaaaaggcag
    37741 cacttttggg cgtttatttg catgggctgg ccggtgattt ggctttacga catcaaagta
    37801 tggaatcctt aactgcaggc gatgtaatag aaaatttggg aagtgctttt caatctttaa
    37861 gagagtaata tagttttgat atattttttt gtatatcaaa aaatcaactc gaaaaattta
    37921 gatttttaaa tataagcata acaaataatt cacagaaaaa acttttttca aaccctcata
    37981 aaaagtaaaa gtattatgac aaagaaaata ttgatttttc tgtttttatc tttttcgata
    38041 gttacgtttg gtcaaaccct aaatcttgat acttgccaga aaagagctaa agaaaattat
    38101 cccttgttga aacaatatgg attgatagaa aagacggccg attacaatat ttccaatgcc
    38161 aataaagcct atttaccaca agtcagcttg ctggcaaaag ctacttatca gtcagatgta
    38221 accgctattc ccgaaacact tggtcaagtt ttaacacagt tgagcgggca accggttagt
    38281 tttcccgtac tgagcaaaga tcaatatcag gccgcattgg aagttgatca gctggtttgg
    38341 gatggcggaa tggtaaatgc tcagaaaaaa ttgataaaag cttcttcaga ggctgaaaaa
    38401 cagaagttgg aagtggattt gtatgcgtta aatgaccgca tcaataattt gtttttcggg
    38461 attatgctca taaaagaaca attgaaggtg aatcagattc ttcagcaaga attggcaagc
    38521 aattatcagc gaatggaggc ttatttgaaa aatggattaa tacaacaagc cgacctggat
    38581 gctgtgaaag tggaacaaat caatgctgca caacaggaaa ccgatttaaa aagtgttctg
    38641 aaatcgtatt gtcaaatgtt gtcggtgttt actggtataa atgtgaatga aaaaacagag
    38701 cttgaaaaac cacttttacc tgttgttaat gagaatattg aaaacaaacg tcctgaactt
    38761 tcactttttg aatcacagaa aaatttgttg gaaagtcaaa agaaaaccat tttagctggg
    38821 aacttgccaa aaataggatt attcattcaa ggtggctacg ggcgaccggg atttgatttt
    38881 ttcaaaaatg attttacttc cttttatatt ggaggtgtga gactttcgtg gaatatagcc
    38941 ggattttatt cacagaaaaa tgaattgaaa aaaatcgaaa ctggtcaaaa aaatgtggat
    39001 attatgaaag aaacttttct tttcaacacc aacttgctta ctaaacaaca aatgaacgaa
    39061 atagacaagc ttcgtgtgac attgaaaaac gatgaagaaa ttatacgttt gcggaacaat
    39121 ataaaaaaag ctacggaatc aaaatttcaa aatggaacaa ttactacaac cgatttttta
    39181 cgtgaaataa atgcagaaaa tatcgcccgt cagaccagaa cacttcacga agttcaacta
    39241 tatatggctg tttatcaatt gaaaaacaca attaacaatt aaaatttgaa aaaacacgga
    39301 aaaatatgga agcaataaaa ttcgacaata aaattttggt tttaagcatg agtttaattt
    39361 tagggttaac atcgtgtcac agacttttgg aaaacaaata cgatgcttcc ggaacttttg
    39421 aagctacgga agtggtggta tcagccgaag caaacggcaa aatcatggat ttcaacctcg
    39481 aagaaggtca acaattgacc gaaaatcagt tgattggcta tatagacagt acgcaattat
    39541 atctgacaaa attgcaattg ctggcttcgc aaaaatctgt ccggagtcga gttccggata
    39601 tcaaaaagca aattgccgtt cttcagcaac aaattgaaac agcgaagaaa gaacaaaaac
    39661 ggattcagaa tctattcagc gccaatgcag ccagccaaaa gcaactcgac gatgtcaatg
    39721 cacaagtttc ggtgctcgaa aaacagcttg aggctgcaaa atcgaatctt gaatcgacca
    39781 gtaatagtat tttgaatgaa aatgaagctt tgaatattca gatagagcaa gtacaaaatc
    39841 aattggaaaa atgtagaatt accagtccga ttaatggaac cgtgctagtg aaatatgccg
    39901 aaaaagggga attggctggt gccggcaaac cgcttttcaa agttgccgac ctgcaaaaaa
    39961 tgattcttcg ggcttatgtt acagccgatc aggttacaca actgaaaatt ggccaaaaag
    40021 caaaagtatt tgtcgatttt ggcaaagatg atcaaaaaga atatgaag
//
LOCUS       DAOTAX010000013        38905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_841_length_38905_cov_32.063089, whole genome shotgun sequence.
ACCESSION   DAOTAX010000013 DAOTAX010000000
VERSION     DAOTAX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 38905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38905
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_38905_cov_32.063089"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..615)
                     /gene="efp"
                     /locus_tag="PLM04_02720"
     CDS             complement(46..615)
                     /gene="efp"
                     /locus_tag="PLM04_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HON02024.1"
                     /translation="MATTADIKNGMCIELDGQYYIITEFLHVKPGKGAAFVRTKLKNV
                     VTGRTIDKTFNAGVKIDEVRIERRPYQYLYQDDMGFNFMNNETFEQVSVNKELINGVD
                     FLKEGMICDVVTHAESETILYVDLPTHVTLQVTYTEPGLKGDTATNATKPATVETGAV
                     IRVPLFINEGELIKVDTRDGSYVERVKTV"
     gene            complement(814..4344)
                     /gene="nifJ"
                     /locus_tag="PLM04_02725"
     CDS             complement(814..4344)
                     /gene="nifJ"
                     /locus_tag="PLM04_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HON02025.1"
                     /translation="MAKQKKFITCDGNQAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAANGRKNIFNEKLQVQEMQSEAGAAGAMHGALQAGALATTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAQALSIFGDHQDVMAVRQTGCAMFATGSVQEIMDMAAVAHL
                     SAIKSRVPFVHFFDGFRTSHEIQKIEQLEQEDLIPLIDQEALKAFRNRALNPEKPVIR
                     GTAQNPDIYFQAREAANPFYDAVPEIVEDYLNKLGEITGRKHGLFDYYGAPDADRVVI
                     AMGSATEAIKEGVDYLNAKGEKVGLISVHLYRPFSAKHFLSVLPETAKRICVLDRTKE
                     PGASGEPLYLDVKDVLYAKSNQPTVVGGRYGLSSKDFTPAQVLAVFENLALPEPKNHF
                     TVGIVDDVTFTSLPLREEIPMGGKGIFEAKFYGIGSDGTVGANKNSIKIIGDNTDKYC
                     QAYFSYDSKKSGGFTCSHLRFGDEPIRSTYLVTTPDFVACHVQAYLRLYDVTKGLKKN
                     GTFLLNTIWDEEEVKKNLPVKVKKYLAAHNINFYIINATKIAQEIGLGNRTNTILQSA
                     FFKISNVIPYELAVEKMKYAINKTYGKKGEDIVNMNYAAVDRGGEYVKVEVPSNWANL
                     EDEKEMEENFPPFIREVVMPINAQAGDDLPVSVFKGREDGTWDSGTAKYEKRGVASFV
                     PVWNSANCIQCNECAFVCPHAAIRPFVLDEAEQAKAPFTDTIKANGKQFVGMTYRIQV
                     DVLDCMGCKNCADICPGTKGNKALTMVPIETQYDNQKNWDFCISEVKSKQHLVDVKMN
                     VKNSQLATPLFEFSGACAGCGETPYVKLITQLFGDRELVANATGCSSIYSASAPSTPY
                     TTNENGQGPAWANSLFEDNAEFGYGMELATEVMRDRLERIMKEAIESNCCSDELKALF
                     AEWIENKEDAEKTKELAAKITPLLKNCDCEHCRKISELSHYLVKKSQWIIGGDGWAYD
                     IGYGGLDHVIASGKNVNILVLDTEVYSNTGGQSSKSTPIGAIAKFASAGKRIRKKDLG
                     MMAITYGYVYVAQIAMGASKAQTLKAIREAEAYNGPSLIIAYSPCISHGIHNGMEKAQ
                     EEQERAVECGYWQLYRYNPDLEAEGKNPFILDSKEPQWEKFQDFLKGEIRYTSLMKQF
                     PAEAQELFVAAENNAKWRYKMYKQMADRQF"
     gene            complement(4390..5571)
                     /locus_tag="PLM04_02730"
     CDS             complement(4390..5571)
                     /locus_tag="PLM04_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON02026.1"
                     /translation="MESFFRTHQYLVEHVQSPVRRTLMDEIDWTDRLIGIKGSRGVGK
                     TTFLLQYAKDFFRSNDQSCLYINFNNLYFTTHTLVEFAERFVKQGGRTLLIDQTFKYE
                     NWSKELRECYERFPELKIIFSGSSVMRLKEDNEDLQDVVKVYNLRGFSFREFLNLQAG
                     TNFQPIDLEDIIKNHVPIANEICSKIDPLLYFQDYLHHGYYPFFLEQRNFSENLLKTM
                     NMMIEVDILLIKQIELKYLSKIRKLLYLLMISAPATPNISQLSCEIQTSRATIMNYIK
                     YLKDARLLNLLYNDNEQFPKKPHLIYVHNTNLMHAAMPGNIDRLSEIKTFLYNSLHAK
                     YKVNSGKYHSDFTVDEQYTFKCELNSNISKQHSKLIFATDQLKIGKKNEIPIWLFGFL
                     Y"
     gene            5884..7287
                     /locus_tag="PLM04_02735"
     CDS             5884..7287
                     /locus_tag="PLM04_02735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON02027.1"
                     /translation="MKIKFIGAAREVTGSKHLITTKDGKRILLDCGMFQGKGLETDAK
                     NKDLGFDPETIDHIILSHAHIDHSGLIPYLYRNGFRGSVICTNATRDLCSIMLADSAH
                     IQELDIKWFNKKRARQGLPPVEPIYDKEDAENCMDLFIGVSLNRRFYIDNKINVKFTN
                     TGHLLGSAVVNLEIIENGKKVHIAYTGDIGRSTDYLLRSPDPFPQCDYLIAESTYGNR
                     LHPETNQTEEDFLKIITETCIKNKGKLIIPAFSIGRTQELVFLLNNFYNRHLLPHIEV
                     FVDSPLSVNATEIYRMYSELMNKNVKEVLKYDNDPFGFNSLHYIKNVEESKALNTYDK
                     PCIIISASGMMEAGRIKHHVANNIENPRNTILIVGYCSPTSLGARIQQPGLREISIFG
                     EIHPVNAKICKIEAFSGHGDYKEMIDYLGCQSAGLIKKTFLVHGDYEVQVEYREKLQK
                     EGFRKVEIPDAKEEFIL"
     gene            7637..7891
                     /locus_tag="PLM04_02740"
     CDS             7637..7891
                     /locus_tag="PLM04_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HON02028.1"
                     /translation="MTKMITFNELRRIKDALPHGSLSQIAEELGLDIETVRNYFGGHN
                     YKDGKSCGIHIEPGPDGGLVMLDNTDILEAALRILGKEEA"
     gene            8131..>8325
                     /locus_tag="PLM04_02745"
     CDS             8131..>8325
                     /locus_tag="PLM04_02745"
                     /inference="COORDINATES: protein motif:HMM:NF028435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HON02029.1"
                     /translation="MNLSPAFEKRMQVLLEEEYALFRAALEKPAPVSIRANNKIVYQP
                     SKEQVSWCESGFYLSERPVFT"
     assembly_gap    8326..8425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8426..9352
                     /locus_tag="PLM04_02750"
     CDS             <8426..9352
                     /locus_tag="PLM04_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HON02030.1"
                     /translation="AKWGNANVVVTNNQPADFKNLPGFFDAMLIDAPCSGEGMFRKNP
                     ASIDEWSLTNVENCVVRQRKIISEVWDTLKTDGILVYSTCTYNRQENEENIKWICEEL
                     GAEILRMDIQHFQGILETEAGYRFFPHKIKGEGFFISVLRKTSPTPQKMRKAKKIEKK
                     SDDKIKSEVGIKLLEPEKWNLLMENNRLRAVSKARQKEIAIIENNLKCLLSGIYLGDY
                     KNSDFIPSAQSALSKETDLKAVETVEIDYTTAVRYLKKENIQVESDKKGFLLLLYKGV
                     PLGWVKNIGQRCNNLYPQEWRIRMKIEELEEI"
     gene            9349..10095
                     /locus_tag="PLM04_02755"
     CDS             9349..10095
                     /locus_tag="PLM04_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON02031.1"
                     /translation="MKILLLGTGTSTGVPQIGCNCYTCTSTNIKDQRLRSSLLVSEKN
                     TRILIDCGPDLRQQLLNYRIHDLTGILLTHEHYDHIGGLDDLRPLGPSHLFGEKRVLK
                     VIERNMPYCFGDKRYPGVPELFLHEINEEEFSLDGLKVQPVRVMHANLPIFGFRIGKM
                     AYLTDVKTLDEKAVEQLQQLDVLIINALRPFKHISHLSLSEAIELAEKINAKETFFTH
                     ISHDMGRHNEINSVLPPHIHLAYDGLELAI"
     gene            10192..11217
                     /locus_tag="PLM04_02760"
     CDS             10192..11217
                     /locus_tag="PLM04_02760"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HON02032.1"
                     /translation="MKKSFIIFVGLSIGAMLFSSCSNMLYTSLDVLRPAKVAFLPNVN
                     RLLIVNNTPVMALENDPNKTKKNVTLPADSLPIYCLSVLAQELGNNSFFSSVLLEPNS
                     IYPKNNYSEKLSTEMVKKLCENYNCDVILTLDSLKASDVLTEYYLEDISQYLSTYEIY
                     LKSKWSIYYPSQSKPVSLQFKDTVYWEAQDYSRQRALKQMPDRWDALVDGALYVGKNS
                     VKRFIPYWDKVDRYFFNPGNKLMRQAMDSVYVRNWTSAIELWKKAMNKSNSEKLKGEA
                     ANNIAIAYEILGDMDNAIAYASQSLQYFSSVSIIDYYTLERINNYYYELIKRKNEIQQ
                     LKLQLGE"
     gene            11291..12817
                     /locus_tag="PLM04_02765"
     CDS             11291..12817
                     /locus_tag="PLM04_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HON02033.1"
                     /translation="MELLLPSDEILLQEKGIGKQQIEEQLHYFETGFPFLKIVAPAVI
                     GNGITIIDDKKGEFYINKWESYRQKEGIDIVKFVPASGAASRMFKDLYEFLESEAELP
                     QKSSVKDFFNQIEKFAFYEELNKACLKNEGKSVAELIKNNRQKTVVENLLLEKGLNYG
                     FLPKGLLLFHSYPDEKRTPVQEHLVEGALYATHADIDVKIHFTVSHQHRALFEKHIQE
                     ILEKYEQKFKVHYHIDFSEQKPSTDTLAVDMNNRPFRENGQLVFRPGGHGALLENLND
                     LLADIIFIKNIDNVVPDSFKEITVKYKKIIAGVLIDIQQLCFDYLKELENNNLTENRL
                     KEILRFTEETLCIRHPEIDSFDTEALKKYLFRKLNRPVRVCGMVKNTGEPGGGPFLTV
                     NADGTISPQILESSQIDLNNPEEKQKLENSTHFNPVDLVCSVKNYKGEKFDLTKYVDK
                     NTGFISIKSKNGRELKALELPGLWNGSMSDWNTVFVEVPIETFNPVKTVNDLLRPQHQ
                     "
     gene            complement(12879..12960)
                     /locus_tag="PLM04_02770"
     tRNA            complement(12879..12960)
                     /locus_tag="PLM04_02770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12924..12926),aa:Leu,seq:tag)
     gene            13292..15730
                     /locus_tag="PLM04_02775"
     CDS             13292..15730
                     /locus_tag="PLM04_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON02034.1"
                     /translation="MKTKLLLSLGLLGTMSFLYGQQYRPGKQIDHDNTAFVRKVELKK
                     AGEVIMKVDQISVTDTTSELSASIYLPDFTLFKNFHAPVPADGAQFFFGHGGVTVAEN
                     LVNNQPRYYFLYQTEGYSEDESGFRFFQDIHIIDENQQQVGKVPTSWMGYQLYMQDGK
                     NMLISDYQDWDTIFWSNPETGWSGWETFQVHTSVVTDVATGQTIFTFPRGTTFSNFNG
                     MQSGIYYATNEQNLFFNIDGEERILTTNNNFEKYFKGHSSGNHENALDMWIYNKDFSL
                     YKKISNVGYDIPIENLYMANTYQPLFGKHAAYFYHTVTYQTDGGLNYNSKTYLVNLNG
                     ELVKIIDGNLNIMSVVYLPQQDKDVALSMNWTNGAIEVLSIPDFEVIGEFNDLIKDKN
                     GNVKFVKYTDDALIIMNEDFSTYKSIPLPDKNTWSFQMVDYYSTCADEKIEFVFTGKE
                     TETGTGKGLLVINEDNETIIDMPDFSALHSSYYADNLPIILRKSTDKYGRPVSDEFIS
                     YYRTGKLPTQIKLGGNPLPGADMQILKQTDNELIALDKITMNADGKGEIELGEGVYYL
                     KALQTQNGLNTYYPNVLLWENATAIPYNTDPLEEGSMYCIEMQSIPVPVNPTAQGVIV
                     GKIVISQPEAVQQLMRIKLVTDNGSVPIFDVYLQNTADNSIIDHTQTDENGNYRFENV
                     PAGSYRVLVNVDGKPMESVNTVTLTTDADIVENVNYEITETGVVAKMPSGLKYTSTAL
                     IIYPNPAKDFIYLPQSAQNAKVQVLDVQGRILLNVDSYKGEKLDVSRLSNGWYMVQIN
                     NGNRIKTGKFIKIN"
     gene            complement(15843..17624)
                     /locus_tag="PLM04_02780"
     CDS             complement(15843..17624)
                     /locus_tag="PLM04_02780"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HON02035.1"
                     /translation="MMQNIKERISALRNEMKINDIAACIIPSSDPHASEYIADYWKER
                     EWISGFNGSAGTVIITLTRAALWTDSRYFLQAENQLQGSGIELMKEGLPETLSWTDWL
                     GEELKNNEKVGLNAQMFSYNAFTHLKNELNKKGISLIAVDLIGKIWKDRPALPSQPFY
                     VYDIRYAGETASHKLASLREQMKKNQADIFVLSALDEIAWLFNIRGSDVDFNPVVIAY
                     AIIKIDKATLFVDSKKITEKTAAFLQEEGITIEPYNDIYSQLKKITEGKRVLIDGSKL
                     NAALYEAIPQHCSIINTMSPVLKMKSVKNKIELEGTRWAMVKDGVALVKFFKWLEENI
                     LSSEISEISAAEKLFECRQQQENFVSESFDTICGYAGHGAIVHYHAEPETDIVLKPDN
                     LLLVDSGGQYLDGTTDITRTIALGIPTQQQKIDYTLVLKGHIALAKAKFPTNTRGSQL
                     DILAHKWLWERGLNYGHGTGHGVGHFLCVHEGPQNIRMDENPAVLLPGMIISDEPGLY
                     RTNAYGIRIENLVLVVEDEKTEFGDFLCFETLTLFPYDRSLIELSMLTEDEINWINSY
                     QKKVLEKLSPYLNEEEKIWLQKKCASL"
     gene            complement(17836..18300)
                     /locus_tag="PLM04_02785"
     CDS             complement(17836..18300)
                     /locus_tag="PLM04_02785"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HON02036.1"
                     /translation="MKKQLKIALVAHDNRKADMVEWAVYNADMLSQHKLICTGTTGKL
                     IQRAMEEKGVKVDITCMHSGPLGGDAEIAALVVQKQINLAVFLIDDLNPQPHEADIQM
                     LLRQCRIHNVPIACNRYSADLMITSTLWDDENYIPTEPKYVKFVREDLFNFE"
     gene            18768..21089
                     /locus_tag="PLM04_02790"
     CDS             18768..21089
                     /locus_tag="PLM04_02790"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HON02037.1"
                     /translation="MNLDFLNDLNESQRQAVEYMDGASLVIAGAGSGKTRVLTYKIAY
                     LLTKGFPPSSILALTFTNKAAREMKERIAQIVGERTARYLWMGTFHSIFSRILRNEAQ
                     YTGYSKNFTIYDSSDSSSLIKSILKERNLDTKTYPPGLIQSRISSAKNNLITPEAYAV
                     NSDFIKSDKQSRIPLLSEIYQTYCNRCKQADAMDFDDLLLQTNLLFRNHPEVLEKYQQ
                     LFQFILVDEYQDTNFAQYLIIKKLAQQHERISVVGDDAQSIYSFRGANIDNILQFKNA
                     YHNARIFKLEQNYRSTQTIVNAANSLIDKNRAQIQKTIFSEKEIGHPIKLLNVFTDYE
                     EGLTVAKHILDLYALNDYRYADIAVLYRTNSQSRILEETFRKQGIPYRIYGGLSFYQR
                     KEIKDVIAYCRLVCNQNDEEALKRIINFPTRGIGETTVGKLVECAQSNDVGIWEVLSN
                     MTKYGVMVNAGTASKLNDFRNLIESFSQQLEIKNAYDLAVTIVKTTGLLNEANFDHSP
                     ESISRKENIEEFLKAIHEFCEKKMEEGETLPLLPDFLSEVSLLTDQDTDKDSLSDKVT
                     LMTVHAAKGLEFKAVFIVGMEEELFPSMFSLVNERELEEERRLFYVAITRAKERCFIS
                     YAKSRFRNGKTNFCTPSRFIKDIDPQFLEITEEKAPTFQKKNFMNWDEEIESERNRFY
                     SPTNEKETYSEPQTINFSPRRLKKLDQSSDEKIIIENSSFPVGTIVKHQLFGIGKVLE
                     TSISNGNEKATIDFGEKGVKSLLLKFAKLEVVK"
     gene            complement(21195..22055)
                     /locus_tag="PLM04_02795"
     CDS             complement(21195..22055)
                     /locus_tag="PLM04_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HON02038.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLISVEEEVELAQRIH
                     KGDRAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPSPEELAE
                     ELDIPVDKIADTMKVSGRHISVDAPFVEGEDNSLLDVMVNEDSPDADHRLINESLSQE
                     IERALSTLTEREHEIIKKFFGIGVPEMTLEEIGDEFGLTRERVRQIKEKAIRRLRNSS
                     KSKLLKSYLG"
     gene            complement(22320..23801)
                     /locus_tag="PLM04_02800"
     CDS             complement(22320..23801)
                     /locus_tag="PLM04_02800"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HON02039.1"
                     /translation="MEASKRKKALGLFLLVVVVSVATNVTTNYFTNKHEKDNFKRELQ
                     YPTAYAKFSNVKSGVETDFTLAAELTIHSVVHVKTQSKAKMAFREDPFFEFFFGRPRY
                     YQEPPMVEGSGSGVIISNDGYIVTNNHVIDGSSKIEVTLNDKRTFEAKVIGADPNTDL
                     ALIKIDAENLPIIPFGNSDSLKVGEWVLAVGNPFNLNSTVTAGIVSAKARNINIINSK
                     MRIESFIQTDAAVNPGNSGGALVNTRGELVGINTAIASQTGSFAGYAFAIPVSIVKKV
                     VSDLRQYGVVQRAVLGVEIADITAQLAKEKDLKTLEGAYVANVNAGSAAEKAGIKEGD
                     VIVNVNGVAVKSVSELQEQIGRYRPGDKISITVIRKNNELKFNNVELKNSQGSASIVS
                     SDKVDVEQLLGATFKELSSKLQKDLGLDYGVQVTSLTKGKLMQQGVRVGYIILKINNQ
                     TIRNVEDIQSAVTAALNSEDKVLFMAGVYPNGRVTYYAVNLEE"
     gene            complement(24062..25351)
                     /gene="eno"
                     /locus_tag="PLM04_02805"
     CDS             complement(24062..25351)
                     /gene="eno"
                     /locus_tag="PLM04_02805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HON02040.1"
                     /translation="MSRIEKIVAREVLDSRGNPTVEVDVMLESGVMGRAAVPSGASTG
                     ENEAIELRDGDKKRYLGKGVLKAVSNVNNIIAPSLIGISALDQRTIDKKMIELDGTKT
                     KSNLGANAILGVSLATAKAAAAYLDLPLYRYIGGVNAFVLPIPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAPSFHEGLRMGAEVFHSLKKVLHDRNLSTAVGDEGGFAPTLKGTEDA
                     LESILTAIEKAGYKPGQDVMIGLDCASSEFYENGIYNYSKFEGANGAKRTSEEQVKYL
                     SELVSKYPIDSIEDGMSENDWEGWKLLTEKLGNKVQLVGDDLFVTNVEFLKKGIEMGC
                     ANSILIKVNQIGTLSETLDAIEMAHRAGYTTVTSHRSGETEDATIADIAVATNSGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGDLAVYGYKKVTKK"
     gene            25522..26283
                     /locus_tag="PLM04_02810"
     CDS             25522..26283
                     /locus_tag="PLM04_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HON02041.1"
                     /translation="MRTTKFILFSFICISLTSCNVLMQGVSNSGTLSIPTTDKENIAG
                     IKNALLLGTERAVSDLSIENGFYGNQAFKLLLPEEAQTIVENISLIPGGKALVDKAIL
                     SINRSAEDAVKEAGPVFKKAIQDMTISDAMGILLGGSNAATQYLQKTTSTELKNLFLP
                     KVQASLKKPLVGDVSTTETWNLLINAYNSAANSTLGKLASLKPVNTQLDQYVTDKALS
                     ALFTKMAETENSIRTDPAARTTTLLKRVFGQLDNK"
     assembly_gap    26427..26526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26548..26856
                     /locus_tag="PLM04_02815"
     CDS             26548..26856
                     /locus_tag="PLM04_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HON02042.1"
                     /translation="MENNENQINIELSAEVAEGIYSNLAIISHSSSEFVLDFIRIMPG
                     LPKAPVKSRIILTPEHAKRLLYALQENIAKYEQQNGKIKIDENSFIPPFPMGFGGGEA
                     "
     gene            complement(26898..28475)
                     /locus_tag="PLM04_02820"
     CDS             complement(26898..28475)
                     /locus_tag="PLM04_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HON02043.1"
                     /translation="MSFQEEIRRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIRKTATSDWMEIEKQRGISVATSVMGFDYQDYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDNAKGVEEQTRKLMNVCRMRKTPVMIFVNKMDREGKDPFDLLDELESELD
                     IHVRPLSWPINQGFSFKGVYNIYEENLQLYTPNKQTISESVEISDIDSEELEKFIGEK
                     DARQLRQDLELILGIYPAFDREKYLSGELAPVFFGSALNNFGVKELLNCFVEIAPSPQ
                     PVQAHERVVNPYEETFSGFVFKIHANMDPNHRSCIAFVKICSGKFERNVNYKHIRQGK
                     MLRFSSPTAFMAQKKEIVDEAYAGDIVGLPDNGNFKIGDTLTSGEDLHFKGLPSFSPE
                     MFNYLENADPMKTKQLEKGIRQLMDEGVAQLFTSEFNRRKIVGTVGQLQFEVIQYRLL
                     HEYNAQCRWEPISLYKACWIESDDAEQLEAFKKRKAQFMVKDKEGRDVYLAESPYILQ
                     MAQRDFDHIKFHFSSEF"
     gene            complement(28850..29266)
                     /locus_tag="PLM04_02825"
     CDS             complement(28850..29266)
                     /locus_tag="PLM04_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02044.1"
                     /translation="MKKILILMVSALFLISVAGHAQNQKNVSDKEGKKSEMRQNAPQR
                     MSAESRAEYWAKELNLTNEEKAKIAELFKKQDETRMQHQAEMQKSRDEHRAFMESQRK
                     ANDAEMEKIIGKEKFQKYDSIRMARMQTMRNRMRNR"
     gene            complement(30610..31650)
                     /locus_tag="PLM04_02830"
     CDS             complement(30610..31650)
                     /locus_tag="PLM04_02830"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HON02045.1"
                     /translation="MKSEESSQITVHGKTVKLFTLTNKNGMSCSITNFGAKVVKLFAA
                     DKEGKFEDVVLGFDTLNECIEREPYFGAVCGRFANRIKDGKFVLDGVEYHLPINNGTN
                     SLHGGIHGFNEKIWEVKSVDNQEIVMEYLSVDGEEGYPGNLTVKVTYFLSDNNELKIH
                     YEAVTDKATVINLTNHSYFNLKGAGNGTIRNHILQLNADFYTVLDDSFAPTGEIRPVE
                     NSAFDFRQPTVIEQRIDDKAFAPGRGLDNNWAIRKHHTGEMVVAGFLYEPESGRKMEV
                     MTTQPGVQVYSGNWIDKQTGKNGKVYESQHAICLETQGFPNSANVPFFPTPVLRPGQK
                     YDEWCIYRFSAE"
     gene            complement(31736..32896)
                     /locus_tag="PLM04_02835"
     CDS             complement(31736..32896)
                     /locus_tag="PLM04_02835"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HON02046.1"
                     /translation="MTQQELKSAFEKIYGQPADAVYFAPGRVNLIGEHTDYNGGYVFP
                     CALSFGTYLLIRKNGGNTVRFHSLNQPNAVEVPLEKLTTRLENSWANYLIGVFAQFIK
                     KGVKIDEGFDILIWGDVPTGAGLSSSASLEVVTAYALNDQLGTGYDRTELALMSQKAE
                     HEFALVNCGIMDQFAAAQGKKDHAIFLNCDTLEFDLVPVKLNGIKVLISNTHSPHKLD
                     SGAYNQRVAECKKAVEQLNKVRSIKYLAELTEEEFKKIESAITDETAKKRARHVVSEV
                     QRTKDAVKALRAGDILQFGKLMNASHVSLRDDYEVTGPELDTMVEEAWKIDGVIGSRM
                     TGGGFGGCTVSLVKDEAIDTFIKNVGAAYEAKIGIKPEFYIAEIGDGACRLS"
     gene            33110..33565
                     /gene="smpB"
                     /locus_tag="PLM04_02840"
     CDS             33110..33565
                     /gene="smpB"
                     /locus_tag="PLM04_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HON02047.1"
                     /translation="MHKTSNIVIKNKRATFEYEILDRYVSGIQLFGTEIKSIRSGKAS
                     LSDTYCTFINDELWVKNMHIAPYFFGSYNNHETRRDRKLLLKAQELKRLQRAVKESGN
                     TIVPLKLFINERGLAKLEIALVKGKKLYDKRQTLKEKEDKRYIERTLKR"
     gene            complement(33607..34212)
                     /gene="yihA"
                     /locus_tag="PLM04_02845"
     CDS             complement(33607..34212)
                     /gene="yihA"
                     /locus_tag="PLM04_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HON02048.1"
                     /translation="MEIHATEFIKSSTDYKGCPEGNFPEFAFIGRSNVGKSSLINMLC
                     QRKNLALTSSKPGKTLLINHFLINKNWYLVDLPGYGFAQTGKENRKKLIDNIKNYILF
                     REQLTCLFVLIDVRHEPQKIDLEFMEWLGSNGIPFCIVFTKTDKLSVSKIDENIENYK
                     QKMLENWEQLPPFFKTSSHKKIGREEILNFIDKIIESLKVQ"
     gene            complement(34219..35685)
                     /locus_tag="PLM04_02850"
     CDS             complement(34219..35685)
                     /locus_tag="PLM04_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HON02049.1"
                     /translation="MSGFAILLIIAAYFSVLLFISFIVSRRSKDNDAFFLGNRKSPWW
                     VVAIGMVGSSISGVSFVSVPGMVNSIDFTYMQTVFGFFFGYLIIANVLLPVYYKLNLT
                     TIYTYLENRFGKHSYKTGASFFLLSKTIGAAARLYIVALILQNLIFEAWGVPFELTVC
                     GIIILIWLYTHQSGIKTIVWTDTLQTIILITALILIIVEVSQQLNMGVKDIYRELVNY
                     PKTRIFVFDDWNDKQNFFKQFFSGIFIAIVMTGLDQDMMQKNLSCHNLQEAKKNMYWY
                     GFSFVPVNFLFLVLGALLLVFSAEKGIALPPLGDEILPMLAANYLGQTVMIFFITGII
                     AAAFSSADSALTALTTSFSVDILGIQHQESRKANHTRRIIHVCISIFFAIIIVIFKAI
                     NNKSVIDAIYTVATYTYGPLLGMYTFGLFTKMQVKDKFMPYIAVLSPILCGLMDYLFT
                     RYLHYSFGYEILIINGLLTFTGMWIFRKKNIIFNTIKS"
     gene            35811..36437
                     /gene="recR"
                     /locus_tag="PLM04_02855"
     CDS             35811..36437
                     /gene="recR"
                     /locus_tag="PLM04_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HON02050.1"
                     /translation="MSQQQFSSSLLEDAIHQFAKLPGIGRKTALRLVLHLLKESEEEV
                     ENFGNSIIRLRKEIVYCKICHNISDSDICDICSNLSRDKSVVCVVENIKDVITIENTQ
                     QFRGLYHVLGGIISPMDGVGPKDLEIDSLVQRVENDGIKEVILALSTTMEGDTTNFYL
                     YRKLEPFHVKITTLARGVSIGDELEYADEITLGRSIQNRIEFSQTFNK"
     gene            36457..36921
                     /locus_tag="PLM04_02860"
     CDS             36457..36921
                     /locus_tag="PLM04_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02051.1"
                     /translation="MIGKIIKTLLLVYYSVYLAAVVAAVVGYVFIFKNYVGIEAQSSI
                     AINLSSAFILFIILSIPLSLGLFHFQVKKWLLIPDKSIRIEKYKKASIYRIIVLGLGF
                     VVGVIFAYLLRSQSIIFCAAMAAAGLIFCKPSEMKMIEELQLDKEENIPAEK"
     gene            36963..37391
                     /locus_tag="PLM04_02865"
     CDS             36963..37391
                     /locus_tag="PLM04_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02052.1"
                     /translation="MIIAVDFDGTIVTHEYPKIGKEIPFAIETLKRLQDEFQHQIILW
                     TVREGRELEEAIEFCKMRGLEFYAINKNYPEEGDDYAESRKIRADLYIDDRNLGGLPD
                     WGVIYQMIAANSVANPFALNGEQPAFRQTKKGFFKSIFGK"
     gene            complement(37439..38473)
                     /locus_tag="PLM04_02870"
     CDS             complement(37439..38473)
                     /locus_tag="PLM04_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HON02053.1"
                     /translation="MKGKNTVLLIFTGGTISMSEDPTTGALRPIDFYRLQEYLPELKQ
                     TGIEIKSIPFSPLMDSSDIQPETWKEIALIIEENFDNFDGFVVLHGTDTMAYSASALS
                     FMLQNLSKPVIFTGAQLPIGMMRSDAKENLLTAIEIATAKENGKAIVPEVCIFFEDTL
                     FRGNRTTKKNAEHFNAFNSYNYPPLAKVGVHIKYFNSYIHYPESFSKLEVKTNINPNI
                     AILKLFPGITQQNVHATLNIPELKAVILESYGAGNAPRLIWLYEELKAAVEKGIIIVN
                     KSQCSTGTVEMGKYETSLNLLNAGVISGYDCTTEALVAKLMHLLGEYHSTEIIKHKLS
                     VSLCGEMTVS"
BASE COUNT        12235 a         7370 c         6933 g        12167 t
ORIGIN      
        1 attttttaca ggaaatattt ttaaaatttc ttttaaattc tatcgttaca ctgtttttac
       61 tctttctaca taagaaccat cacgggtatc gactttaatt aattctcctt cgttaataaa
      121 aagtggaacc cgaattacag cacctgtttc cactgtggcc ggctttgtgg cattggttgc
      181 ggtatcgcct ttcaaacccg gttcggtata ggtaacttgc aaggtaacgt gagtgggaag
      241 atcaacataa aggattgttt cgctttctgc atgggttacc acgtcacaaa tcattccttc
      301 tttcaaaaaa tctactccat tgattaattc cttatttacg gaaacttgtt caaatgtttc
      361 gttattcata aaattgaatc ccatatcgtc ctgataaaga tattgatagg gacggcgttc
      421 tatacgtact tcatcgattt ttactcccgc gttgaaagtc ttatctattg ttctgcctgt
      481 aactacattt ttcaattttg tacgtacaaa agcagctcct ttgccgggtt taacgtggag
      541 aaattccgta atgatataat actgtccatc aagttcgatg cacatgccgt ttttaatgtc
      601 tgcagtagta gccatagatt tttttattca aaggtaaatg aatgatttta tatattttga
      661 agataaccga tctgtttata aaaaaggttt gcaaatttac tacttttctc ttaattttca
      721 aaatgcttat aaaacactca ttgtcagata aataaaaaaa gagctgctca ttaaaacttg
      781 aacagccctt cgttaattta ttgatgaatt ttatcaaaat tgtctgtcag ccatttgttt
      841 atacatttta taacgccatt tagcattgtt ttcggctgca acaaacaatt cctgagcttc
      901 ggcagggaac tgtttcatca aggaagtata acgaatttcg cctttcagga aatcttggaa
      961 tttttcccat tgcggttctt tagaatcgag tataaaagga ttttttcctt ctgcttccaa
     1021 atcgggattg taacgataca gttgccaata accgcattca acagcgcgtt cctgttcctc
     1081 ctgagctttt tccattccgt tgtgaatgcc gtgactgata catggtgaat aagcaatgat
     1141 cagcgaaggc ccattgtaag cttctgcttc gcgaattgct ttgagcgttt gtgccttgct
     1201 ggctcccata gcaatttgag ctacatacac ataaccataa gttatggcca tcatgcccaa
     1261 atcttttttg cggattcttt ttccggcaga agcaaatttg gctatcgcgc caatgggcgt
     1321 tgattttgac gattgtccac cagtattgga ataaacttct gtatccaaaa ccaaaatatt
     1381 gacattttta ccggatgcta taacatgatc caatcctcca taaccgatgt cgtaagccca
     1441 accatcacca ccaataatcc attgcgattt ttttacgaga taatgggaaa gttccgaaat
     1501 tttccgacaa tgttcacaat cacaattttt gagaagcggc gtgattttgg cagccaattc
     1561 tttggttttt tcagcgtctt ctttgttttc aatccattcg gcaaaaagtg ctttcagctc
     1621 atcagaacag caattgcttt ctattgcttc tttcatgatt ctttcgagtc tgtcgcgcat
     1681 tacttcggtg gccaattcca ttccgtaacc gaattcggca ttatcttcaa acaacgaatt
     1741 tgcccacgct ggcccttgtc cattttcgtt ggtagtataa ggcgttgatg gagcagaagc
     1801 agaataaatg gaactacaac ccgttgcatt ggctaccaat tctctgtcgc caaagagctg
     1861 agttattaat tttacgtatg gcgtttctcc acaaccggca cacgctcctg aaaattcaaa
     1921 caatggtgtt gccaactgcg aattcttcac attcattttt acatccacca aatgctgttt
     1981 ggatttcact tccgagatac agaaatccca atttttctga ttgtcatatt gggtttctat
     2041 cggtaccatt gtaagcgcct tattgccttt tgttcctgga caaatatcgg cacaattttt
     2101 acagcccata cagtcgagaa catcaacttg aatgcggtaa gtcatgccca caaactgttt
     2161 cccgttagct tttatcgtat cggtgaaagg cgctttggcc tgttcggctt cgtccaaaac
     2221 aaaaggtcga atagcagcgt gaggacaaac gaatgcacac tcgttacact gaatacagtt
     2281 cgcagaattc caaacgggaa cgaaactagc tacgcctcgt ttttcatatt ttgcagttcc
     2341 ggaatcccaa gttccatctt cgcgtccctt gaaaacggag acaggcagat catcgccggc
     2401 ctgagcattt attggcatta ccacttcacg gatgaaagga ggaaagtttt cttccatttc
     2461 cttttcatct tccagatttg cccagttgct tggaacttca actttaacat attctcctcc
     2521 gcgatcaaca gcggcatagt tcatgtttac tatgtcttca ccttttttgc cgtaagtctt
     2581 gttaatagca tatttcattt tttcaactgc caattcgtaa ggaataacat ttgaaatttt
     2641 gaaaaatgct gattgaagaa tagtattggt tctgtttccc aatccaattt cctgagctat
     2701 tttggtagca ttgataatgt aaaaattaat gttgtgtgcg gccagatatt ttttaacttt
     2761 taccggaaga ttttttttca cttcttcttc gtcccaaatt gtattgagca ggaaagttcc
     2821 gtttttcttc aaacctttgg taacatcgta aagtctgaga taagcctgca catggcaagc
     2881 aacaaaatcg ggagtcgtta ccagataagt ggaacgaatt ggttcgtcgc caaagcgaag
     2941 atgagagcaa gtgaaaccac ctgatttttt agaatcgtaa gaaaaatagg cttgacaata
     3001 tttatcggta ttatctccaa ttattttaat tgaatttttg tttgcaccca ctgttccatc
     3061 cgaaccgata ccgtaaaatt ttgcttcaaa tattcctttt ccacccattg gaatttcttc
     3121 ccttagcgga agagaagtaa aggtaacatc atccactatg ccgacggtaa aatgattttt
     3181 tggttcggga agagccaaat tttcaaaaac ggccaaaacc tgagccggcg tgaaatcttt
     3241 ggaagaaagt ccgtatctac cacccacaac ggttggttga ttggatttag catacaatac
     3301 atcctttaca tcaagataaa gaggttcgcc ggaagctccc ggttctttgg tgcgatccaa
     3361 cacgcaaata cgttttgctg tttcgggtaa taccgacaga aaatgttttg ccgagaaagg
     3421 acgataaaga tgtacagaaa tcagtccgac tttttcgcct tttgcgttga gatagtccac
     3481 tccttctttg atggcttcag ttgccgatcc cattgcgata acaactctat cggcatccgg
     3541 agcgccataa taatcaaaca agccatgttt tctgcccgta atctctccca gtttgttcag
     3601 gtaatcttct acaatctccg gaactgcatc atagaaagga ttggcagctt cgcgagcctg
     3661 gaaataaata tcgggattct gagcggtacc tcttatgaca ggtttctccg gattaagtgc
     3721 gcgattgcga aaagctttca aggcttcctg gtcaattaat ggaattaaat cttcttgttc
     3781 caactgctcg attttctgaa tttcgtgtga agtacggaat ccgtcaaaaa agtgaacaaa
     3841 aggaacgcgt gatttgatag ccgataaatg agccacagca gccatatcca taatttcctg
     3901 aaccgaacct gtcgcaaaca tggcgcagcc ggtttgtcga actgccatta catcttgatg
     3961 atcgccgaaa attgataaag cctgtgcggc aatagaacga gccgaaacat gaaaaacgcc
     4021 cggcaacagt tctccggaaa ttttgtacat attgggaatc atcagcagca atccttgcga
     4081 tgcagtgaaa gtagtagcca aagctcctgc ctgcaaagct ccgtgcattg ctccggcagc
     4141 tccggcttcc gattgcattt cctgtacttg aagtttttcg ttgaaaatat tttttcttcc
     4201 gtttgctgcc cattcatcaa catattctgc catagttgaa gaaggtgtga tgggataaat
     4261 ggctgccact tcgctaaaca tataagcgat atgggcggct gcctgatttc catcacaagt
     4321 tatgaatttt ttctgttttg ccattgtgtt gtgatttatt tgggttttta ctatagttta
     4381 ttatttggtt tagtataaaa aaccgaagag ccaaattgga atttcattct ttttcccaat
     4441 tttcagttga tcggttgcaa aaattaattt tgaatgttgt ttggaaatat ttgaatttaa
     4501 ctcgcattta aacgtatatt gttcatcaac agtaaaatcg gaatgatatt ttccggagtt
     4561 aactttgtat tttgcatgca gcgaattgta caaaaaggtt tttatttctg ataatctgtc
     4621 gatatttccc ggcatagcgg catgcattaa gtttgtattg tgaacatata tcaaatgagg
     4681 ttttttagga aattgttcat tgtcgttata aagcaagttc aaaagtcggg catctttgag
     4741 atactttatg taattcatga tagttgctct cgaagtttga atttcgcaac tcaactggct
     4801 tatgttcgga gttgcagggg cactgatcat gagtaaataa agcaattttc tgatttttga
     4861 aagatatttc aattcgattt gtttgatcag aagaatatca acttcaatca tcatattcat
     4921 ggttttcagc agattttccg aaaaatttcg ctgttccaaa aaaaacggat aataaccatg
     4981 atgcagataa tcttgaaaat agagcaaagg atcaatttta ctgcaaattt catttgctat
     5041 cggcacatga ttttttataa tatcttccaa atcaataggt tgaaagtttg tcccggcttg
     5101 caaattcaaa aattccctaa aggaaaaacc tcttagatta tatactttta caacgtcttg
     5161 taaatcttca ttgtcttctt ttaaacgcat tactgacgat ccgctgaaaa ttattttcag
     5221 ttcggggaaa cgttcataac attcacgcaa ttcctttgac caattttcgt atttgaaagt
     5281 ttgatcaatc aacaaagtac gtccgccctg tttcacaaaa cgctctgcaa attctaccaa
     5341 agtatgggta gtaaagtaca aattgttgaa attgatatat aaacaacttt gatcattcga
     5401 cctgaaaaaa tcttttgcat attgcagcaa aaaagtagtc tttccaactc ctctacttcc
     5461 ctttatccct atcaaccgat cggtccagtc tatttcatcc atcaaagttc gtctcacagg
     5521 agattgtaca tgttcaacca aatattgatg cgttctgaaa aatgactcca tcacgtataa
     5581 attttgtttg caaaaatagc aatatttatc tgattttgca aagtggagat gacaatttta
     5641 gcctttttat ttttattggt taagttttgg gttaagaaaa ctttaataaa atttattttt
     5701 ttagttggtt agggtttaaa tgtataattg ttgataaaaa attggataga aatttctgtc
     5761 aataacaata atcatttact tttgaaaaat aaaaggctct tttttatcaa cagaaatttt
     5821 ttaagtaaac ttaaaaaatt ccaaaaaaag gattaaaaac caaagattaa aatcattaat
     5881 tatatgaaaa tcaagtttat aggagcagca cgtgaagtta caggcagcaa acatttaata
     5941 accacaaagg atggaaaacg gatattgctc gattgtggaa tgtttcaggg aaaaggattg
     6001 gaaaccgatg ccaaaaataa agatttgggt ttcgatcccg aaacaataga tcatattatt
     6061 ctttcacatg ctcatattga tcattccgga ctgattccct atctctatcg aaatggtttt
     6121 cgtgggtcgg tcatttgtac caatgcaacc cgtgatcttt gttccattat gttagccgat
     6181 agcgctcata ttcaggagct tgatattaaa tggtttaata aaaaacgcgc acgtcagggt
     6241 ttgccgccag tggagcccat ttatgataag gaagatgcgg aaaattgcat ggatttgttt
     6301 attggagttt cactcaatcg ccgtttttat attgacaata aaattaatgt gaaatttacc
     6361 aataccggac atttattggg aagtgcagtt gtgaatctcg aaataattga aaatggaaaa
     6421 aaagttcaca ttgcctatac cggggatatt ggtcgttcaa ccgattactt gttgcgttca
     6481 ccggatccct ttcctcaatg tgattacctg attgccgaat caacttatgg caaccgtctt
     6541 catcctgaaa caaatcaaac ggaagaagat tttcttaaaa ttataactga aacctgtatc
     6601 aaaaataagg gaaaattgat cattccagcc ttttctattg gacgaaccca ggaattggta
     6661 tttctgttga ataattttta taatcgacat ttacttcctc acattgaagt ttttgttgac
     6721 agtcctctat ctgtgaatgc tacagaaatt tatcgaatgt acagcgaact aatgaataag
     6781 aatgtgaaag aagtgctcaa atacgacaat gacccatttg gattcaattc cttacactac
     6841 ataaaaaatg tggaagaatc aaaagcttta aatacctatg acaaaccctg tataattatt
     6901 tctgcctcgg gtatgatgga agcaggacgt atcaaacatc acgtggccaa taatattgaa
     6961 aatccgcgaa atacgatctt aattgtaggt tattgttcgc ccacttcttt aggtgctcgc
     7021 attcagcaac ctggcttaag ggaaatatcg atatttggag aaattcatcc cgttaatgcc
     7081 aaaatttgca aaattgaggc attttcaggt catggcgatt ataaagaaat gattgattat
     7141 ctgggttgcc aatcagcagg cttgattaaa aaaacttttc tagtgcacgg ggattatgaa
     7201 gttcaggtag aatatcgtga aaaattacaa aaagaaggtt tcaggaaagt tgagatacct
     7261 gatgcaaagg aggaatttat actgtaggaa aatttttaaa gttgaaaacc taacaagtta
     7321 acttaatcca aattaattga ctatcataaa tttaagtggc tatgaagcca ctacaaatac
     7381 tcctttaatt gttaaatgtg cctgtttatt agttcttcta agtttttata gaaatgttta
     7441 aatagatgaa ttaacaaaat gaattaacag ggaaaacata aatatttgaa acacattttt
     7501 ttaaccacct taaattaaaa attcaatttt ttccgtgaaa ttttcaaaaa aagtttcttc
     7561 atattaaaag aatatcctaa atttagagcg caatatatca aagtaaaaat ttataaacat
     7621 ctaatactta attgtcatga caaaaatgat aacattcaac gaattgcgta gaattaaaga
     7681 tgcgcttcca catgggagcc tgagtcagat cgccgaagaa cttgggttgg atattgaaac
     7741 agtacgaaat tactttggcg gtcataatta caaagatggc aaaagttgcg gaattcatat
     7801 tgaaccgggc cctgatggcg gtttagtcat gttggataac acagatattc ttgaggctgc
     7861 tttacgaatt ttggggaaag aagaagctta aaagtgcaaa aggaaaagga aaaaagaaga
     7921 aaaagaaatt gaaaggcagt gaaccagcat tcgctgcttt tttttaatcc gatgcttaaa
     7981 aaatcattcg tccaaaaatc gtcccattat ctataatgta ttcatttttc atcattttaa
     8041 gcaatatttc ctatatcatt ataaaacttc ctgtcttatt tatacaaaaa agactaaatt
     8101 tgcaacttac aaccaacaat ttcatttcgc atgaatcttt cgccggcttt tgaaaaaaga
     8161 atgcaagttt tattggaaga ggaatacgct ttgttcagag cagcattgga aaaaccggct
     8221 ccggtaagta tcagagcaaa caacaaaatt gtttaccaac cttccaaaga acaagtgtca
     8281 tggtgtgaaa gtggatttta tctgagcgaa cgcccggttt ttacannnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnngcaaa atggggaaat gccaatgtgg tggttaccaa
     8461 taatcaaccc gctgatttta aaaatcttcc cggctttttc gacgccatgt taatagatgc
     8521 accctgttcg ggagaaggaa tgttcagaaa aaatcccgct tcgattgatg aatggtcgtt
     8581 gacaaatgtt gaaaattgcg tcgttcgaca acggaaaatt atctccgaag tctgggatac
     8641 tttaaaaacc gacggcattc ttgtttatag cacctgtact tacaaccgtc aggaaaacga
     8701 agaaaacatc aaatggattt gcgaagaact cggcgctgaa attttgagaa tggatattca
     8761 acattttcag ggtattctgg aaacggaagc cggttatcgt ttttttccac ataaaataaa
     8821 gggggaaggt tttttcattt ccgttctacg caaaacttct cctacaccgc aaaaaatgag
     8881 gaaagcaaaa aaaattgaaa aaaagtcgga tgacaaaata aagtccgaag tcggaataaa
     8941 acttcttgaa ccggaaaaat ggaatttgtt gatggaaaac aatcgtctcc gcgcagtaag
     9001 caaagccagg caaaaagaaa ttgctatcat tgaaaataat ttaaaatgcc tgctttcagg
     9061 tatttatttg ggcgattata aaaattccga ttttatccct tctgctcagt ctgctttatc
     9121 caaagaaacc gatttgaaag cagtcgaaac ggttgaaatt gactatacaa ctgccgttcg
     9181 ttatttaaag aaagaaaaca ttcaagttga atctgacaaa aagggttttt tattgcttct
     9241 ttataaaggt gttcctttgg gatgggttaa aaatatagga cagcgatgca acaaccttta
     9301 tcctcaggaa tggagaataa gaatgaaaat tgaagaatta gaagaaatat gaaaattttg
     9361 ttattaggca ccggcacttc tacaggcgta cctcaaattg gttgcaactg ctatacctgc
     9421 acttccacca acattaaaga tcaacggctg cgttcttcct tgctggtaag cgaaaaaaat
     9481 accagaattt tgattgactg cgggcccgat ttacgacagc aattattgaa ttatcgcatt
     9541 cacgatttaa ccggtattct gcttacccac gaacattacg accacattgg cggacttgac
     9601 gatcttcgtc cgcttggtcc ttcgcatttg tttggcgaaa aaagagtttt gaaagttatt
     9661 gaacgaaata tgccttactg ttttggcgat aaacgttatc cgggtgtacc cgaattgttt
     9721 cttcatgaaa ttaacgaaga agaattttca cttgatggtt taaaagttca acctgttcgt
     9781 gtcatgcatg ccaatttgcc tatttttgga ttccgaatcg ggaaaatggc ttaccttacc
     9841 gacgtcaaaa cgctggatga aaaagctgtt gaacaacttc aacaattgga tgttttaata
     9901 ataaatgcct tgcgaccttt taaacatatt tctcatttat ctttgtcgga agccatcgaa
     9961 ttggcagaaa aaattaatgc caaagaaact ttttttaccc atatcagcca cgatatggga
    10021 cgacataacg aaataaattc ggtacttccg ccccatattc atttggctta cgatggattg
    10081 gagcttgcaa tttgagacag agagaaattc agagaaatta ttaaaattat cttctcgcaa
    10141 actaaatgcg cacattttcg tttgatttat gaaaaaaata attaaagcca gatgaaaaaa
    10201 agttttataa tatttgttgg tttatccatt ggggccatgt tgtttagttc ctgttcaaac
    10261 atgctctata cctcgctcga cgtactcaga ccggctaaag ttgctttttt gcccaatgtt
    10321 aaccggcttt tgatagtaaa caacacacct gtcatggcat tggaaaacga tccgaacaaa
    10381 accaagaaaa atgttacgct tccagccgac agtttgccaa tttactgctt aagcgtattg
    10441 gctcaggaac ttggtaacaa cagctttttt tcatccgttc tgttggaacc taactccatt
    10501 tatccaaaaa acaattattc cgaaaagctt tcaaccgaaa tggtaaaaaa actttgtgaa
    10561 aattacaact gtgatgtaat tctgacattg gacagtctca aagcttcgga tgtgttgaca
    10621 gaatattatc tcgaagacat tagccagtat ttaagtacgt atgaaattta cctcaaatca
    10681 aaatggagta tttactatcc ttcgcaatcc aaaccggttt ctttacagtt taaagataca
    10741 gtatattggg aagctcagga ttattcgcgg cagagagctt tgaagcaaat gcccgacaga
    10801 tgggatgcat tggttgatgg cgctttgtat gtaggaaaaa attcggtaaa aagatttatt
    10861 ccttattggg ataaagttga tcgttatttt ttcaatcccg gcaacaagtt aatgcgacag
    10921 gcaatggatt ctgtttatgt aagaaattgg acttcggcca ttgaattgtg gaaaaaagcc
    10981 atgaacaaat ccaatagcga aaaactcaaa ggtgaagctg ccaacaatat tgccatcgcc
    11041 tatgaaattc ttggcgatat ggataatgcc attgcgtatg ccagccagtc tcttcaatat
    11101 ttttcttctg tttccattat tgattattac acactggaaa gaatcaacaa ctattactac
    11161 gaactgataa aacgaaaaaa tgaaattcaa caactgaaat tgcagttggg agaatgattt
    11221 ttttgagcaa tggagcaaca atcataaaaa tcaattcaac ttattgataa ccaacaaaaa
    11281 acgaaaaaat atggaactac ttttgccttc tgatgaaatt ttattacagg aaaaaggaat
    11341 cggcaaacag caaatcgaag aacaattgca ttattttgaa acaggattcc cttttttgaa
    11401 aattgttgca ccggccgtaa ttggcaacgg aattacaatt atcgatgata aaaaaggaga
    11461 attttacatt aacaaatggg aaagttacag acaaaaagaa ggaatcgata tagttaaatt
    11521 tgttcctgcc tctggagcgg catcccgaat gtttaaagat ttgtacgaat ttttggaaag
    11581 tgaagcagaa ttacctcaaa aatcttctgt aaaagatttt ttcaatcaaa ttgaaaaatt
    11641 tgccttttac gaagagttaa acaaagcctg cctgaaaaat gagggaaaaa gtgttgccga
    11701 attgataaaa aacaacagac aaaaaacggt agtagaaaat ctgttgcttg aaaaaggttt
    11761 gaattacggc tttctaccga aaggtttgtt gctttttcat tcctatcccg acgaaaaaag
    11821 gactccggtt caggaacatt tggttgaagg agctttgtat gctacccatg ccgacatcga
    11881 tgtaaaaatc cattttacag tttcacatca acacagagct ttatttgaaa aacacattca
    11941 ggaaattctg gaaaaatatg agcaaaaatt caaagtacat tatcatatcg acttttctga
    12001 acaaaaaccc agtaccgaca cattggctgt agatatgaac aatcggcctt tcagagaaaa
    12061 cgggcaactg gtatttcgtc cgggaggtca tggcgcttta ctggaaaacc tgaacgacct
    12121 acttgccgat atcattttca taaaaaacat cgacaatgtg gtgccggatt cctttaaaga
    12181 aattaccgtc aaatacaaaa aaattatagc gggagtttta attgatattc aacaactgtg
    12241 cttcgattat ttaaaagaac tggaaaacaa taatttaacc gaaaatcggt tgaaagaaat
    12301 acttcgattc acagaagaaa ctctttgtat tcgtcatccc gaaatcgatt cttttgacac
    12361 cgaagcatta aaaaaatatc tgttccgtaa attaaatcga cctgtgaggg tctgcggaat
    12421 ggtaaaaaac acgggagaac ccggcggtgg tccattcctc accgtaaatg ccgacggaac
    12481 aatttctccg caaattttgg aaagttcaca aatcgacctg aacaatccgg aagagaaaca
    12541 aaagttggaa aattctacac atttcaatcc ggtagatttg gtttgctcgg taaaaaatta
    12601 caagggagaa aaatttgatt taacgaaata tgtcgataaa aataccggtt ttatctccat
    12661 taagtcaaaa aacggaaggg aactgaaagc gctggaactt ccgggtttgt ggaacggttc
    12721 aatgagtgat tggaatactg tttttgtaga agttccgatt gaaactttta atccggtgaa
    12781 aaccgtcaac gatttgctac gccctcaaca tcaataaaaa accaataagg atactaaaaa
    12841 accggttatc aataaaattt gataatcggt tttttttgtg cggctgaagg gactcgaacc
    12901 cccacgcctt tcggcaccag atcctaagtc tggcgcggct accaattacg ccacaaccgc
    12961 ataaggatta ttggctgcga agataaggaa attttcagga tattacaagt ttttacggtt
    13021 tgtaaaggaa atgatttcct gagaattaat taataaattt tattcattta tctgataatc
    13081 aaagtattaa tttaatttgc aaccaagatg tattgtctgt ttgattgacg ggagagaaaa
    13141 atcgatatga aaaatttgaa ttaaaatcga gcgtaaacaa gatttttacg atttgcctga
    13201 aaatttttac caaatgctta cctatcgcat taagttttga ctatttttgt ttcaaactat
    13261 tttagataca ctaacttaaa ttaataatgc tatgaaaaca aaactacttt tatcattggg
    13321 tttattggga acaatgtcct ttttgtatgg tcagcaatac agacccggaa aacaaataga
    13381 ccatgataac actgcttttg tacgaaaagt cgaattaaaa aaagcgggag aagtcattat
    13441 gaaagtcgat caaatttctg ttaccgatac aacttctgag ctttcggcat cgatttattt
    13501 acctgatttt acacttttta aaaattttca tgctcccgtt ccggctgacg gagcacaatt
    13561 tttcttcgga cacggaggcg taactgtggc tgaaaacttg gtaaacaatc agcctcgtta
    13621 ttattttctt tatcaaacag aaggatatag tgaagacgag tccggtttta gattttttca
    13681 agacattcat atcatagacg aaaatcagca gcaagtgggg aaagtgccaa cttcgtggat
    13741 gggttatcaa ctctatatgc aagatggtaa aaacatgctg atatccgact atcaggactg
    13801 ggatacgata ttttggagca atcctgaaac cggatggtcg ggttgggaga cttttcaggt
    13861 gcatacctct gttgttactg atgtagcaac agggcaaacc atttttactt tcccacgagg
    13921 cactactttc tcgaacttca acggaatgca atcggggata tactatgcaa caaatgagca
    13981 aaatctgttt ttcaatattg atggagaaga aagaatactc actaccaata acaattttga
    14041 aaaatatttt aaaggacatt cctcaggaaa tcatgaaaat gcccttgaca tgtggattta
    14101 caacaaagat ttttcattgt ataagaaaat ttcaaatgtg ggatatgata ttcccatcga
    14161 aaacttatat atggccaata cctatcaacc acttttcgga aaacatgctg cttactttta
    14221 tcacacggtt acctatcaaa cagatggagg cttaaattac aacagtaaaa catacctggt
    14281 aaatctgaat ggagagttgg ttaaaattat cgacggaaat ctcaatatca tgagcgtggt
    14341 atatttacca caacaggaca aagacgtggc attgagcatg aattggacaa atggggcaat
    14401 agaagtttta agtattcccg actttgaagt aattggagaa tttaacgatt tgattaaaga
    14461 caagaatgga aatgtaaaat ttgtaaaata taccgacgat gcattgataa tcatgaacga
    14521 agattttagt acttacaaat ctattccctt gcctgacaaa aacacatgga gtttccaaat
    14581 ggtagattat tattcaacgt gtgcagacga aaaaatagaa tttgtattta ccggaaaaga
    14641 aactgaaact ggaactggaa aaggattgct ggttatcaat gaagataacg aaaccattat
    14701 tgatatgcca gattttagcg cactccattc ttcttattat gctgataact tgcctattat
    14761 tttaagaaaa tcaacagaca aatatggtcg tccggtatcg gatgaattta tctcctacta
    14821 tcgcacaggc aagttgccaa cccaaatcaa acttggtggc aatccattgc caggagccga
    14881 tatgcaaata ctgaaacaaa ccgataatga gcttattgca ctcgataaaa ttacgatgaa
    14941 tgctgacgga aaaggagaaa ttgaattggg tgaaggtgtt tattacttga aggcactgca
    15001 aacccaaaac ggattgaata cttactatcc caacgtgctg ttatgggaaa atgcaactgc
    15061 cataccttac aataccgatc cgcttgaaga aggaagtatg tattgcattg agatgcaatc
    15121 tattccggtt cctgtaaatc caacagcaca aggagttatt gttggtaaaa ttgttatcag
    15181 tcaaccggaa gcagttcagc agttgatgcg aataaaactg gttaccgaca atggttctgt
    15241 tccaattttc gatgtttatt tgcaaaacac agccgacaat tccattattg accatacaca
    15301 aaccgatgaa aatggaaatt atcgtttcga aaatgtgccg gcaggttcgt atcgagtttt
    15361 ggtgaatgtt gacgggaaac caatggaatc cgtcaataca gtaacattga caaccgatgc
    15421 cgacattgtt gaaaatgtaa attatgaaat taccgaaacc ggtgttgttg ccaaaatgcc
    15481 ttccggattg aaatatactt caacggcgct gataatctat ccgaatccgg ccaaagattt
    15541 catatatctt ccacagtcgg cacaaaatgc caaagttcaa gttttggatg tgcaaggaag
    15601 aattttattg aatgtcgaca gttataaagg agaaaagctt gatgtgtcaa gactttcaaa
    15661 tggttggtac atggttcaaa taaataatgg gaacagaata aaaacaggta aattcattaa
    15721 aatcaactag cgcttaaaac aactatactg aaaaaaatat ctggcttaca gcaatataag
    15781 tcagatattt ttataaaatt caatttgcaa acagattaaa caatactctt tttttaaaga
    15841 aattacaaag aagcacattt tttctgcagc caaatcttct cttcttcatt caaataagga
    15901 gaaagctttt ccaaaacttt tttctgataa gaatttatcc aatttatttc gtcctcagtg
    15961 agcatggaaa gttcaatcag cgaccgatcg taaggaaaaa gtgtgagtgt ttcgaaacac
    16021 agaaaatcac caaactcagt tttttcgtct tccacgacca gcacgagatt ttcaatgcgt
    16081 atgccataag cattggttcg ataaagaccc ggctcgtcgg aaataatcat tcccggcaaa
    16141 agtacggcag gattttcatc catccgtata ttttgtggcc cttcgtgaac acaaagaaaa
    16201 tgtccaacgc catgtcctgt tccgtgccca taattcaaac ccctttccca gagccatttg
    16261 tgcgcaagaa tgtcgagctg ggatccacgc gtattggtag ggaatttagc ttttgcaagt
    16321 gcaatatgac ctttcaatac aagcgtataa tctatttttt gttgctgagt gggaattccc
    16381 aacgctatgg ttcgggtaat atcggtagtt ccatccaaat attgaccacc cgaatcgaca
    16441 agtaagagat tatcaggctt taaaacgata tcggtttcag gttcggcatg ataatgaaca
    16501 atagccccat gaccagcata accgcaaatg gtatcgaaac tttccgaaac aaaattttcc
    16561 tgttgttgcc tgcattcgaa caacttttct gcagcggaaa tttcgctgat ttctgaagat
    16621 aaaatatttt cttccagcca cttgaaaaat tttaccagcg caacaccatc ttttaccatt
    16681 gcccatcgag taccttccaa ttctatttta ttttttacac tcttcatttt caaaaccggc
    16741 gacatagtat tgataatgct gcaatgttgc ggaatagctt catacaacgc agcattcaac
    16801 ttggagccat caatcaaaac tcgtttccct tcagtaattt ttttcaattg ggaatagatg
    16861 tcgttgtacg gctctatggt tatgccctcc tcttgaagaa aggcagcggt tttttccgta
    16921 atttttttcg aatcgacaaa aagggtagcc ttatcgattt ttattattgc ataagctatt
    16981 accaccggat taaaatccac atcgctccct ctaatgttga acaaccaagc aatttcatcc
    17041 aaagccgaaa gcacaaaaat atctgcctga ttttttttca tctgttcgcg cagggaggcc
    17101 agtttatggc ttgccgtttc acccgcataa cgaatatcgt aaacataaaa aggctgagaa
    17161 ggcaaagcag gtcggtcttt ccaaatttta ccaatcaaat cgacagcaat cagtgaaatt
    17221 cccttcttgt tcaattcgtt tttcagatgg gtaaaagcat tgtaagaaaa catttgggca
    17281 tttagtccca ctttttcatt atttttcagt tcttctccca accagtcagt ccagctcaaa
    17341 gtttccggaa gtccttcctt cataagctct atgccggagc cttgcaactg attttctgcc
    17401 tgtaaaaaat atcgcgaatc ggtccacaac gcagctcttg tcaaagtaat tatcactgta
    17461 ccagccgaac cgttaaatcc cgaaatccat tcgcgctctt tccaataatc ggcaatatat
    17521 tcgctggcat gaggatcgct actcggaata atacaggcag ctatatcatt tattttcatc
    17581 tcgttgcgca atgccgagat tctttctttt atattttgca tcagtattca ttttaatttt
    17641 tccctgttga tgcggaaaat ttttatatta acattttaaa aatgaaataa ttatctgaaa
    17701 gtaaaaaaat tattgttaag tactttcatc agaatataat ttatttttta aaggtctgat
    17761 ggaacccaca tgtctgaata ttatattcat tcgtgtttgt gtatgcaata catgtgggtt
    17821 tcataaatta aaatttcatt caaagttaaa taaatcttct ctaacaaatt tcacatattt
    17881 gggttcagtc gggatgtaat tttcatcgtc ccacagcgtg ctggtaatca tcaaatcggc
    17941 actgtaacga ttgcaggcaa tgggaacatt atgaatcctg cactgacgga gcaacatttg
    18001 gatatcggct tcgtgtggct gtggattcaa atcatcaatc agaaaaactg ccagattaat
    18061 ctgtttttgt accaccaatg cggcaatttc cgcgtcgccg cccaaagggc cggagtgcat
    18121 gcaagtaata tccaccttca cacctttttc ttccatagcc ctttgaatga gttttcctgt
    18181 cgtaccggta caaatcaatt tgtgttgcga aagcatatcg gcattgtaaa cagcccattc
    18241 taccatgtcg gcttttcgat tatcatgagc taccagcgca attttcaatt gttttttcat
    18301 aagttttatg ttttttgatg aaagaattta ttaaagtttt caatgaaaat ctcagttgaa
    18361 ggttgaatca ttttttcgat tcagaatcga tcgaattaat gaaaaataaa aaattcaatc
    18421 tataaaaaca ggaatttcaa acattaatag aaataaattt tttgctgttt tcgaatgttt
    18481 tttcagaaaa aaccttataa tcctaatggt tgcaaaaata aagaataaat atttatttcg
    18541 gacaaggaaa atgaaaaaac ttttataata acatcatagc gcataaaact catttgatta
    18601 tttcaagctg tttatatctc gttaaatttt taaacaaagc tttcaaccga aaagtactta
    18661 tggtttcata aaaattgtct ttaaaaaccg attcaatttg ctaactttgc ataaagaaaa
    18721 ccggtaagat ttaaagtttc tacaaaaata tttggatgaa caaatcaatg aatttggatt
    18781 ttttaaacga tttgaacgaa agtcagcggc aagctgtaga atatatggat ggcgcttcgc
    18841 tggtaatagc gggtgcaggg tcgggaaaaa cccgcgtgtt aacttacaaa attgcctatt
    18901 tactcaccaa aggttttcct ccgtcgtcca ttttggcttt aactttcacc aacaaagcgg
    18961 cacgagaaat gaaggaacgg attgcacaaa ttgttggaga aagaacagcc cgatacttgt
    19021 ggatgggaac ttttcactcc attttttcca ggattttgcg taacgaagca cagtacaccg
    19081 gatattcaaa aaatttcacc atatacgact cttccgattc ttccagcttg ataaaaagca
    19141 ttctgaaaga acgcaatctg gatacaaaaa cttatccgcc cggactcatt caatcacgca
    19201 tttcttcggc aaaaaacaac ctgattacac ccgaagccta tgccgtcaac agcgatttta
    19261 ttaaaagtga caagcaaagc cgcattccgc ttttgagcga aatttatcaa acttattgca
    19321 atcgttgcaa acaagccgat gcaatggatt tcgacgattt actgctccaa actaatctgc
    19381 tgtttcgaaa tcatcctgaa gtgctggaaa aatatcaaca attgtttcaa tttattttgg
    19441 ttgacgagta tcaggatacc aactttgcgc aatacctgat tataaaaaaa cttgcccagc
    19501 aacacgaacg aattagtgtt gtgggtgacg atgcacaaag catttattca tttcgcggtg
    19561 ccaacatcga caacattcta cagttcaaga atgcttatca caatgcacgc atcttcaaac
    19621 tcgaacagaa ttatcgttcc acacaaacca tcgtcaatgc agccaacagc ctgattgaca
    19681 aaaacagggc acaaattcaa aaaaccattt tttcggaaaa agaaatcgga catcccataa
    19741 aattgttgaa tgttttcaca gattatgaag aaggtctgac cgttgccaaa catattttgg
    19801 acttgtacgc gctaaacgat tatcgatatg ccgacattgc tgtgttgtat cgaaccaatt
    19861 cacaatcgcg aattttagaa gaaacattcc gaaaacaggg cattccctat cgaatttacg
    19921 gaggcctgtc gttttatcag cgaaaggaaa taaaggatgt gatagcttat tgccgattgg
    19981 tatgcaacca aaatgatgaa gaagcattga aaagaatcat taattttccc acgcgaggca
    20041 ttggcgaaac tacagtagga aaacttgtgg aatgtgcaca gagtaatgat gtaggaattt
    20101 gggaagttct ttccaacatg acaaaatacg gcgtgatggt caatgccgga acagcttcaa
    20161 aattaaacga tttccgaaat ttgatagaaa gtttttccca gcaactggaa attaaaaatg
    20221 cttacgactt ggccgttacg attgtaaaaa ccaccggatt gctcaacgaa gccaactttg
    20281 atcattctcc tgaaagtata agcagaaaag aaaatataga agaatttctg aaagccatcc
    20341 acgaattttg tgaaaaaaaa atggaagaag gagaaacctt gccccttctt cccgattttt
    20401 tatcggaagt atctttattg accgaccagg acaccgacaa ggacagcctg agcgacaaag
    20461 ttaccctcat gaccgttcat gcagccaaag gattggaatt taaggccgta ttcattgttg
    20521 gtatggaaga agaacttttc ccgtcaatgt tcagccttgt caatgagcgg gaactagaag
    20581 aagagcgccg ccttttttac gttgccatta cccgtgccaa agaacgctgt tttatcagct
    20641 atgccaaatc gagatttaga aacggtaaaa ccaacttttg cactcccagc agatttatca
    20701 aagatatcga ccctcagttt ctggaaatta ccgaagaaaa agcaccaact tttcagaaaa
    20761 aaaattttat gaattgggat gaggaaattg aaagtgaacg gaatcgtttt tactcgccaa
    20821 caaacgaaaa agaaacttat tcggaacctc aaacaataaa tttttctcca cgaagactga
    20881 aaaaattgga tcaatcgtcg gacgaaaaaa tcattatcga aaattcttct tttcctgtag
    20941 gaactattgt aaaacatcag cttttcggaa ttggaaaagt attggaaacc agcatttcca
    21001 atggtaacga aaaagcaacg atcgattttg gagaaaaagg agtaaaatcg cttttactga
    21061 aattcgcaaa actggaagtg gtgaaataat tatcgaagag tatttactga tgccttaatg
    21121 ggttaaggaa taaaaaaagc gtcctttttg gggacgccct ttgcattcat tttaaaataa
    21181 gcattttatg aaaattaccc gaggtaactc ttcaataatt tacttttaga actgtttctc
    21241 aatctacgga tagctttttc cttgatttga cgaacacgct cgcgtgtcag tccaaattca
    21301 tcgccgattt cttccaacgt catttcaggt acgccaatcc caaagaattt tttaataatt
    21361 tcatgctctc tttctgttaa tgttgacaga gccctttcaa tttcctggga aagtgattca
    21421 ttgatcaaac gatggtcggc atcgggagaa tcttcattta ccatcacatc gagcaggctg
    21481 ttgtcttccc cttcaacaaa aggcgcatct acggaaatgt gacgacctga aaccttcatg
    21541 gtatcggcaa ttttatccac cggaatatcg agttcttcag ccaattcctc aggtgaagga
    21601 cgacgctcat tttcctgttc aaattttgaa aaagccttgt tgattttgtt caacgaacct
    21661 acttggttta atggcaaacg aacaatacgc gactgctcgg ccaacgcctg caaaatggat
    21721 tgcctgatcc accacactgc ataggaaata aacttaaatc cgcgtgtttc atcgaatttt
    21781 tcggcagcct tgataagtcc aagatttcct tcattgatca aatcgggcaa acttaagccc
    21841 tgattttgat actgttttgc taccgaaacg acaaaacgca aattggcgcg ggttaatttt
    21901 tccaaagcgg ctcgatctcc cttgtgaatc cgttgagcca actcaacttc ttcttccaca
    21961 ctgatcagat cttctcttcc aatttcttgc aagtacttgt cgagcgatgc gctttcgcga
    22021 ttcgtgattg attttgtaat ttttaattgc ctcattttta ccttaaaaaa ttagggtgca
    22081 aaattaatgt ttttttggtt gattacaaaa tttcttatat aacgttttta ctaaattttt
    22141 gttttaaaaa gtcttgaaaa tttttctttg ttgttccata aatacgccga aacgatattt
    22201 tatgagttgc ctgattgttg ataatctcaa attttagtac aaaaggaact tactcctttc
    22261 gtaaaaaaga gtaagttcca atgcaacaga gaaatatttt tcaacacact tatggaaaat
    22321 tattcttcca aattaactgc atagtaggta actctgccat tgggataaac acccgccatg
    22381 aaaagcacct tgtcttcgct gtttaatgcc gcagttacgg cagattgaat atcctccaca
    22441 tttctaatag tctgattgtt aatttttaat ataatatatc caactctcac accttgctgc
    22501 atcaattttc cttttgttag cgatgtaacc tgaacaccat aatccaaacc aagatctttt
    22561 tgcaacttac tgctcaattc cttgaaagta gcacccagaa gttgttcaac gtcaactttg
    22621 tcggaagaaa ctatgctggc acttccctga ctatttttca gttctacgtt gttaaatttc
    22681 aattcattat ttttacgtat aacggtgatt gaaattttat cgccggggcg atagcgtcca
    22741 atttgttcct gcaattccga aacagattta acagccacgc cattcacatt cacaattaca
    22801 tcaccttctt taatgcctgc tttttcggca gcacttccgg catttacatt ggccacataa
    22861 gctccttcca atgttttcaa atctttttct tttgccaact gtgcggtaat atcagctatt
    22921 tcaactccca acacggctcg ttgtactact ccgtactgac gcaaatcgga aactactttt
    22981 ttaactatgc tgaccgggat ggcaaatgcg taacctgcaa acgaacccgt ctgagaagct
    23041 attgccgtat tgatacctac caattcgccc cgtgtattga ccaaagcgcc tccactatta
    23101 cctggattaa ccgcagcatc ggtctgaata aaagattcga tacgcatttt ggagttaatg
    23161 atattgatat ttcttgcctt tgcactcaca attcccgccg taacggtaga attaagattg
    23221 aaaggattcc ccactgccag cacccattcg ccaactttta gcgaatcgga attaccaaaa
    23281 ggaatgatgg gaagattttc tgcatcgatt tttatcagcg ccaagtctgt attaggatcg
    23341 gcaccaatta cttttgcctc aaatgttcgt ttatcgttta atgtaacttc tattttggac
    23401 gaaccgtcaa ttacatggtt gttggttaca atatatccat cattggaaat tatgacgcca
    23461 gagccagaac cttctaccat aggcggttct tgataatagc gaggacggcc aaagaaaaat
    23521 tcaaaaaagg gatcttccct aaaagccatt tttgcttttg attgtgtttt tacgtgtact
    23581 accgaatgaa tcgtaagttc agctgccaat gtaaaatcgg tttccacacc actcttcaca
    23641 ttggaaaatt ttgcataagc tgtcggatac tgcaattctc gtttaaaatt atctttctca
    23701 tgtttattgg taaagtaatt ggttgtcaca tttgttgcca cactcactac taccaccaac
    23761 aagaataggc ctaacgcttt ttttcttttc gatgcttcca tacttttcgt tttttagatt
    23821 taaattgatt tttctgaaaa aatattctta aacaaatttt tcactgctaa agtatacaaa
    23881 atacattcca taacacaaag tgatatagct atttgtcgaa atttaacaca gagttttgca
    23941 gcttaacggg aattaacaga aagacctcag attttttaga aaaattttgt tactaaaata
    24001 aaaaaccacc tttttgacag gtggttctgg attgaaaaca aaaaactgac aatttatact
    24061 gtcatttttt tgtaactttt ttgtagccat aaacggccaa gtctcccaat tcttcttcga
    24121 tacgaagcag ttggttatat ttggccatac ggtctgaacg gctcaaagaa ccggttttga
    24181 tctgaccgga attggttgct actgcgatgt cggcaatggt agcatcttcc gtttcacccg
    24241 aacgatgaga agttaccgtt gtatatccgg cacgatgagc catttcgata gcatccaaag
    24301 tttcggacaa tgttccaatc tgatttacct tgataagaat ggaattggca caacccattt
    24361 cgataccttt tttcaaaaat tctacattgg taacaaacaa atcgtcccca accaactgaa
    24421 ccttgtttcc aagtttttcg gtcaatagtt tccagccttc ccagtcgttt tcactcatac
    24481 cgtcttcaat agaatcaatc ggatatttgc tgaccaattc tgataggtat ttaacctgtt
    24541 cttcagaagt acgtttggcg ccatttgctc cttcaaattt ggagtaatta taaataccgt
    24601 tttcgtaaaa ttctgacgaa gcacaatcca acccaatcat cacatcctgt ccaggcttgt
    24661 aaccggcttt ttcgattgca gttaaaatag attccagcgc atcttctgtc cctttcaaag
    24721 ttggggcaaa tccgccttca tcaccaacag ctgtgctcaa attacggtcg tgcaacactt
    24781 ttttcagtga atgaaacact tcggctccca ttcgtaaacc ttcgtggaaa gaaggagcac
    24841 caaccgggcg aatcataaat tcctggaacg cgatgggagc atcagaatgc gaacctccat
    24901 tgataatgtt catcattgga atcggcaaaa caaaagcatt tacaccacca atatatcggt
    24961 aaagcggcag atcgagataa gctgcagcag cttttgcagt agccaaagaa actcccaaaa
    25021 tggcatttgc acccaaattc gactttgttt tcgtaccatc gagctcaatc attttcttgt
    25081 caatagtacg ctgatccaat gcagaaatac caattaatga aggtgcaata atgttattta
    25141 cgttcgaaac ggctttcagt acgccttttc ccagatagcg ttttttatcg ccatcacgca
    25201 actcaatggc ttcattttct cccgtagaag ctccggacgg aacagcagct cttcccataa
    25261 cgcccgattc gagcattaca tctacttcta cagttggatt tcctcttgaa tccaatactt
    25321 cgcgagcaac gattttttct attctactca ttttctttat aattaagatt gataatttgt
    25381 tagaaattcc tctttttaca ttttctgcaa agttataaaa tgttctttta attttgttta
    25441 tatacgtact taaaatatca aaaaatagcg tatatttacc ttcttaaaaa tgaaaattaa
    25501 atattttgca acaataaagc gatgagaaca acaaaattta ttttatttag cttcatttgt
    25561 atttccctta cttcatgtaa tgttcttatg cagggtgttt ccaattcagg aacattatct
    25621 attccaacca ccgacaaaga aaacattgcg ggtataaaaa atgctttgtt attgggaacg
    25681 gaaagagcgg tatccgattt aagtatagaa aatggctttt atggtaatca ggcatttaaa
    25741 ttactgttgc ctgaagaagc tcaaacgatt gtagaaaata tcagtttgat acccggaggc
    25801 aaagctttgg tcgacaaagc cattctgtcc atcaatcgtt cggcagaaga tgcagtaaaa
    25861 gaagcaggtc ctgtgtttaa aaaagcgatt caggacatga ctatttcaga tgcaatgggt
    25921 attttgctgg gcggaagcaa tgcagcaacg caatatttgc aaaaaactac ttcaacggaa
    25981 ttgaaaaatc tttttctacc taaagttcag gcatcgctaa agaaaccatt ggtaggtgat
    26041 gtttcaacta cagaaacatg gaatttgctg ataaatgcct acaacagcgc agccaattct
    26101 actttgggta aacttgcttc tctgaaacct gttaacaccc aattggatca atatgttacc
    26161 gacaaggcat tgagtgcatt gtttactaaa atggcagaaa ctgaaaattc cattcgcacg
    26221 gaccctgcag cccgcactac aacattactg aaacgcgttt tcggacaact ggacaataaa
    26281 taattcattt gctggattat acaatttttt ctctctttag tacatagaat gctaaaatag
    26341 tttcttcaca cacataaata ttttgctact tttgcaacgc ggtttcaaca aaggtgaaaa
    26401 ataaattaat taaaatataa gacaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnaatt aattaaaata taagacaatg gaaaacaatg aaaatcaaat caatatagaa
    26581 ctttctgctg aagtggcaga aggtatctat tccaatctgg ctattatttc acattcttct
    26641 tccgaatttg tgctcgattt tattcgcatt atgcccggat tacccaaagc tcccgtcaaa
    26701 tcacgcatca ttctcacccc cgaacatgcc aaaaggttat tgtatgcact gcaggagaat
    26761 attgcaaaat atgaacagca aaacggaaaa ataaaaattg atgaaaattc ttttattccc
    26821 ccttttccta tgggatttgg aggcggagaa gcctaatttt tctacgcaaa ttccatcttt
    26881 cttagttgaa atagaaatta aaattcggaa gaaaaatgga atttgatatg atcaaaatcg
    26941 cgttgagcca tttgcaaaat atagggcgac tcggcgagat atacatcgcg gccttctttg
    27001 tctttcacca taaattgcgc ttttcgtttc ttgaaggctt cgagctgttc agcatcatcg
    27061 ctttctatcc aacacgcctt atataaggaa atcggctccc aacggcattg cgcgttgtat
    27121 tcgtgcaaca atcggtattg aattacttca aactgcaact gcccgactgt tcccactatt
    27181 tttcttcgat taaattcgct ggtaaaaagt tgtgctacgc cttcgtccat cagttgccga
    27241 attccttttt ccaattgttt ggttttcatc ggatcggcat tttcgagata attgaacatt
    27301 tcgggcgaaa aactcggtaa tcctttaaaa tggagatctt ctccactggt cagcgtgtcg
    27361 ccaattttaa aatttccatt atcgggcaat cccacaatgt cgccggcata agcttcgtcc
    27421 acaatttcct ttttttgtgc cataaaagca gttggacttg aaaaacgcaa catttttccc
    27481 tgtcgaatat gcttgtaatt gacatttcgt tcaaattttc ccgaacaaat tttcacaaaa
    27541 gcaatacaac tccgatgatt gggatccata ttggcgtgaa tcttgaaaac aaaacccgaa
    27601 aaagtttcct cgtaaggatt tacaactctc tcgtgagcct gaacgggttg cggagaagga
    27661 gctatttcca cgaagcaatt caacaattcc ttcactccga aattgttcaa agcgctgcca
    27721 aaaaaaaccg gagccaactc tcccgacaga tatttttccc tgtcgaaagc cggatatatt
    27781 cccagtatga gttctaaatc ctgcctcagt tggcgtgcat ctttctctcc aatgaacttt
    27841 tccagctcct cactgtcaat atccgaaatc tcaacggatt cgctgatagt ttgcttattg
    27901 ggggtgtaaa gctgaagatt ttcctcgtag atattataaa ctcccttaaa tgaaaaaccc
    27961 tgattgatag gccaactcaa aggacgaacg tgaatatcga gttccgattc cagctcatcg
    28021 agcaaatcga atgggtcttt gccttcacga tccattttat tgacaaaaat catgaccggc
    28081 gttttgcgca tccggcacac attcattaat ttgcgcgttt gttcctctac ccctttggcg
    28141 ttgtcaatca ctataatcac gctgtcgacg gccgtaagcg ttctgtaagt atcttcagca
    28201 aaatcctgat gacccggtgt atccaaaata ttaattttgt aatcctgata atcaaatccc
    28261 attacggaag tagctacaga aattccacgc tgtttttcta tttccatcca gtctgaagtt
    28321 gccgtttttc tgattttatt cgatttcacg gcaccggcaa catggatagc tcctccaaaa
    28381 agcaaaagtt tttctgttaa ggtggttttt ccggcgtcgg gatgactgat gatggcaaat
    28441 gttcttctgc gtcgaatttc ttcttgaaaa ctcattttga atacgataga taaattcttt
    28501 attacttgtt tttagcctgc aaagttacaa ctttttgaaa aattttcagt ctgctttaaa
    28561 aggaataaat tttctattgg ttgaaaaaat ttccccaaaa aattgtgttg tatattatga
    28621 aacccacatg tattagatac acaaacactt attcagacat gtggggtcca tcagaccatt
    28681 aaaaaataaa ttatattctg atgaattaaa aaaaagaggc tatcctattc tttagacagc
    28741 ctcgttacct taaaaattac ctatatactt caacacaatc ttttttttca atgtctaaca
    28801 aattttgagc caaaattcaa gattcttatt aaaaactttt agaaaaaaat tatcgattcc
    28861 tcattctgtt tctcattgtc tgcattcttg ccatacgaat agaatcgtat ttctgaaatt
    28921 tttcttttcc aatgattttt tccatttcgg catcatttgc ttttcgttga ctttccatga
    28981 atgcccgatg ttcatctctt gatttttgca tttctgcctg atgttgcatt cttgtttcat
    29041 cctgtttttt aaacaattct gcaattttgg ctttttcttc attggtaaga ttcagctctt
    29101 tagcccaata ttctgcacga ctttcagccg acattctttg tggtgcattt tgtctcattt
    29161 cacttttttt cccttctttg tcagaaacat ttttttgatt ttgagcatga cctgccacac
    29221 ttatcaaaaa taaagcacta accatcaaaa ttaaaatctt tttcataaac ttttgttttt
    29281 aaaattctta ttttctttaa attttataat ttgacttatg aatcaagtca aggtttaaaa
    29341 tcaaaatatt ttttatagaa aattaacaat ttatgtttgc tgtaaaattt gaaacctcaa
    29401 tagagaaaaa ataatggttt tgtcatacag cgaacacagt acagtttcct gttccaatcc
    29461 atcattctaa caacacatgt tgcaacgcaa ttcaataggg aaacaaacgc gcattccccc
    29521 aattcagttt atgcgtttca ttctatcatt gcaataacat ttttggatgt tgtatatgag
    29581 acgtagcatg ttacgtcttt acccggatta aacgcaattc aatggggaaa atatccttac
    29641 attttgctgc cgctcaattg aatcgtgtgg tagaaatttc caaagtaaag catttacccg
    29701 accaaattat caagagcagt tttactttcc tttttaataa ctttggaatt aaccgactta
    29761 gaaaaaactt ggaacaactg cgaaaagact tgtaatggct gacagcctaa ttttttagaa
    29821 acacttattg aaagatgaat aacttcaaaa cttttcatgt acttactcat gtcgttttga
    29881 taaacttaga gttcgcaata ggggaaaaag ttttaatttt ataggaaact cttatgagag
    29941 tagcaataga aaaaaacgtg tgtgaacttt ggtatatcct tacaaacaaa gaaataacac
    30001 catgtggtgg aatgattttt ttgtaacaaa tgtttgaaaa accacgtaga atcgttattg
    30061 ttcgtcaaaa attaaaggaa agcactttcg tctttaattt tgtgatgata gcctataatc
    30121 ttatgtcgct gtttagaatg ttcattttgc agagaaaaac gcagaaaact ttatctacac
    30181 acttagatat aaaacttttg ccattggtgc atattttgaa aaaaatcaac aacaaactgc
    30241 ttctgtaaat cgctttaaat aaaaaaggcg aacatgattt tccggcctat ggaacagttc
    30301 ttgtaagttt gactttccct ttaaattggc taatgagtaa tttgggttca attccaacaa
    30361 tatcaaaatc gtctaatatc tctttcggaa gaatctaacg ggcaagtgcc tcaagctgtt
    30421 cgtaagtgga catataaaaa gtattttttc acaaagatac ttctcttttt ctacaaactc
    30481 atatttttgc aggcgagcca taaacgacag aaattagaaa aaaatctctc taatttctgt
    30541 cgttaaaatg tttccctatg atttgaaaag catcgactta caggaaaaat ataatttgtt
    30601 ttcccttcct tattccgccg aaaatctgta aatacaccac tcatcgtatt tttgtccggg
    30661 acgtaatacg ggagtgggga agaagggtac attggctgaa ttgggaaatc cttgcgtttc
    30721 gagacaaatg gcatgttgac tttcatagac ttttccgttt ttccccgttt gtttgtctat
    30781 ccagttgccc gaataaacct gcactcccgg ttgtgttgtc atgacttcca tttttctgcc
    30841 cgattcaggt tcgtacagaa atccggctac aaccatttct cctgtatgat gtttccgaat
    30901 agcccaattg ttgtccaatc ctcttcccgg agcaaaagct ttatcatcga tgcgctgttc
    30961 aattacagtg ggttgacgaa aatcgaaagc cgagttttct acgggacgaa tttctcctgt
    31021 cggtgcaaaa gaatcgtcca gtacggtgta aaaatcagca ttcaactgta aaatgtggtt
    31081 gcgaatagtt ccgttgccgg ctcctttgag attgaaatag gaatgattgg tcagattgat
    31141 gaccgttgct ttatcggtta cggcttcgta atgaattttt aactcgttgt tgtccgaaag
    31201 gaaataagtt acctttaccg tgagattgcc tggataacct tcttcgccat ctaccgacag
    31261 atattccatt acaatttcct gattgtcaac cgatttaact tcccagattt tttcgttgaa
    31321 cccatgaatg ccgccatgaa gagaatttgt tccgttgttg atgggcaaat gatattcaac
    31381 gccatcgaga acgaatttgc catctttaat tcggttggca aagcgaccac aaacagctcc
    31441 gaagtaaggt tcgcgttcaa tacattcgtt taatgtatca aaacccaaca ccacatcttc
    31501 aaattttcct tctttatcgg cagcaaaaag cttgaccact tttgctccaa agttggtgat
    31561 gctgcatgac atcccgtttt tattagttaa agtaaatagt tttactgttt taccgtgaac
    31621 tgttatttgt gaagattcct cgcttttcat gtctaactcc tgtttaaatt gcagttattg
    31681 aaatatcata aatcatttca ataaatatta ggtttttgtt ttgattgaaa ataaatcagc
    31741 tcaatcgaca agcgccatca cctatttcgg caatataaaa ttcgggtttg attccgattt
    31801 ttgcttcata agcggctccc acatttttaa taaaagtgtc tatcgcttca tcttttacca
    31861 gagaaacggt acatcctcca aagcctccgc cggtcattcg tgaacctatc acgccatcta
    31921 ttttccaagc ttcttctacc atcgtatcga gttcagggcc ggtaacttcg taatcgtcgc
    31981 gaagagaaac atgagaagca ttcattaatt tcccaaactg tagaatatcg ccggctctca
    32041 aagcttttac cgcatctttg gtgcgttgaa cttcgctcac aacgtggcga gcacgttttt
    32101 ttgccgtctc gtcggtaatc gccgattcta tttttttaaa ttcttcttcc gtcagttcgg
    32161 ccagatattt tattgaacga accttattca attgctcaac tgctttttta cattcggcaa
    32221 cgcgttggtt ataagctccg gaatccagct tatgggggct gtgtgtattg gaaatcagaa
    32281 ctttaatacc atttaatttt acgggaacca aatcaaattc cagtgtgtcg caattcagga
    32341 aaatggcatg atcttttttc ccttgcgcag cagcaaactg atccataatg ccacaattta
    32401 caagggcaaa ttcatgttct gccttttgcg acatcagagc caattctgtg cggtcatatc
    32461 ccgttcccag ttgatcattc aatgcgtaag ccgtaacgac ttccaatgaa gcagaagaag
    32521 acaatccggc accggttggc acatcacccc aaatcaatat atcaaaacct tcatcgattt
    32581 tgacaccttt tttgataaat tgcgcaaaaa caccaatcag ataatttgcc cacgaatttt
    32641 cgagtcgagt agttaatttt tccaaaggaa cttctacggc attcggctga ttcaaagaat
    32701 ggaaacgaac tgtatttccg ccatttttac gaatgagcag ataagttcca aaactaagtg
    32761 cacaagggaa aacataaccg ccattataat cggtatgttc accaattaaa tttacccttc
    32821 ccggagcaaa ataaactgca tccgcaggct gaccgtaaat tttttcaaaa gctgatttta
    32881 attcctgttg agtcatatta agtaagtttt ttatagtttc tgaatgcaca aatttaggtt
    32941 aaatcagaat atttatctgt ttaaaaaata tgttttaaaa cagaataatc tgattgtttt
    33001 gtgcatctga tggtgattaa aagaattata tgtaattttg aattcccaat tttttcttgt
    33061 ttgttttaga agttgtaaac aacaattatt tgtcaaaaat tttaatgcga tgcataaaac
    33121 atccaatata gtgattaaga acaaacgtgc cacttttgaa tacgaaattt tagatcgtta
    33181 tgttagcggt attcaattgt ttggaacaga aattaaatct attagaagtg gcaaggcaag
    33241 tctatccgat acatattgca catttattaa tgatgaatta tgggttaaaa atatgcacat
    33301 agctccctat ttttttggta gctacaataa ccatgaaact cgaagagacc gtaaattatt
    33361 gttaaaagcg caggaattga aacgacttca acgtgcagta aaagaaagtg gcaatactat
    33421 cgttccctta aaacttttta tcaatgaaag aggtttggca aaattggaaa ttgctttggt
    33481 aaaaggcaag aagctttatg ataaaagaca aacattaaaa gaaaaggaag ataaaagata
    33541 catagaaaga actttgaaaa ggtaatatgt ttttttaacc tcttttcttt gttaagtgca
    33601 gagattttat tgcactttca ggctttcgat tattttatcg ataaaattta atatttcttc
    33661 tctaccgatt tttttatgtg aggaggtttt aaaaaatgga ggcaactgtt cccaattttc
    33721 caacattttt tgcttgtaat tttcaatatt ttcgtcgatt ttcgatactg acagtttatc
    33781 ggttttagta aaaacgatgc aaaaaggaat tccatttgat cccaaccatt ccataaattc
    33841 aagatctatt ttttgtggtt catgacgcac atcaatcaac acaaacaaac atgtaagttg
    33901 ttcacgaaaa agaatataat ttttgatatt atctattagt ttttttcggt tttcttttcc
    33961 ggtttgagca aatccataac ctggcaaatc taccaaatac cagtttttgt taattaaaaa
    34021 atgattgatc aacagtgttt taccgggttt cgaagaagta agggccagat tttttcgctg
    34081 acaaagcata ttgatgagag atgattttcc cacattggaa cgccctataa atgcaaattc
    34141 aggaaagttc ccttcaggac accccttgta gtcagtactg ctttttataa attctgtcgc
    34201 atgtatttcc atttctattt atgactttat ggtgttaaaa attatgtttt tctttctgaa
    34261 gatccacata cctgtaaaag taagtaatcc atttattatg agtatttcgt atccgaaaga
    34321 ataatgtaag taacgcgtaa ataaataatc catcaatcca catagaatag gagataagac
    34381 tgcaatatat ggcatgaatt tgtcttttac ctgcatcttg gtgaaaagtc cgaaagtata
    34441 cattcccaac aaaggtccat aagtataggt agctacagta taaatggcat caattacgct
    34501 tttattgttg atagctttga aaatcacaat aattattgca aaaaatatgg aaatacaaac
    34561 atgaatgatg cggcgtgtgt gattagcttt tcggctttct tgatgctgaa tgcccaaaat
    34621 atctaccgaa aaagaggtgg ttagggctgt aagtgcagaa tcggcactgg aaaaggcagc
    34681 ggcaataata cctgtaataa aaaaaatcat tactgtttga ccaagataat ttgcagccaa
    34741 cataggcaga atttcgtcgc ccaatggagg caaggcaata cccttttcgg cggaaaaaac
    34801 aagcagcaat gcgcccaaaa caagaaacag aaaattaacc ggtacgaaag aaaatccgta
    34861 ccagtacatg tttttctttg cctcctgcaa attgtgacac gataagtttt tttgcatcat
    34921 atcctgatca agtcctgtca taactattgc aatgaaaata ccgctaaaaa attgtttaaa
    34981 gaaattttgc ttgtcattcc aatcatcaaa aacaaaaatt cttgttttgg gataattaac
    35041 aagttctctg taaatatcct tcacacccat attcaattgt tgggaaacct caacaataat
    35101 gagtatcagt gccgtgatta aaataatagt ttgtagcgta tctgtccata caatggtttt
    35161 aattccgctt tgatgggtgt aaagccaaat gagtatgatg attccacaaa cagtaagttc
    35221 aaaagggact ccccaagctt caaaaataag attctgtaaa atcagtgcaa caatatatag
    35281 tcgagcagca gcaccaatag ttttcgatag tagaaaaaaa gatgctccgg ttttgtaaga
    35341 gtgcttgcca aaccgatttt caagataggt ataaatggtg gtaagattga gtttataata
    35401 tacaggaagt aatacattgg caataatcag atagccgaag aaaaaaccaa atactgtctg
    35461 catgtaagta aaatcaatgc tgtttaccat gcctggtaca gaaacaaaac ttacacccga
    35521 aatagaagaa cccaccatgc cgatggcaac aacccaccag ggcgatttac gatttcccag
    35581 aaaaaaagca tcgttatcct tgcttcttct gcttacaata aaagaaataa acagtagaac
    35641 cgaaaaatat gcggcaataa tcaataaaat tgcaaatcct gacatgcttg ttatcatttg
    35701 aattttcgag tggcaaattt agacaaaaaa ctttaaagta acttcccctc ttactcttag
    35761 tttgttggga aaaaattact aaatttgcag aaagtttgta ttatctgttt atgagtcaac
    35821 aacaattttc ctcttcgctc cttgaagatg ctattcatca gtttgccaaa ctacccggta
    35881 ttggtagaaa aactgctctt cgattggtgc ttcatctgct taaggaatcg gaagaagaag
    35941 tggagaattt tggaaattcc attattcggc tacggaagga aattgtgtat tgtaaaattt
    36001 gtcataatat ttccgatagc gatatttgcg atatttgcag taatctttca agagacaaat
    36061 ccgtagtttg tgtggtagaa aatatcaaag atgttattac gatagaaaat acgcagcaat
    36121 ttcggggttt atatcatgtg cttggcggaa ttatttcacc aatggatggc gttgggccta
    36181 aagatttgga aatcgacagt cttgttcaaa gagttgaaaa tgatggcata aaagaagtaa
    36241 ttttggcatt gagtaccaca atggaaggcg atacgaccaa cttttatctt tatcgtaagc
    36301 ttgaaccctt tcatgtaaaa atcactacgc tggctcgtgg agtatctata ggagacgagt
    36361 tggagtatgc cgatgaaatt acgcttggac gctccattca aaaccgtatt gaattcagtc
    36421 agacgtttaa taaataaatt tttttgttca gttcgtatga taggaaaaat aattaaaaca
    36481 cttttgcttg tatattattc tgtttatctg gctgccgttg tggcagcagt ggtgggatat
    36541 gtatttattt ttaaaaatta tgtcggtata gaggctcaaa gttctatagc aatcaatctt
    36601 tcctcagcat tcattttgtt tattatttta tcgattccct taagtcttgg actttttcat
    36661 ttccaagtaa aaaagtggtt gcttattccc gataaaagta ttcggataga aaaatataaa
    36721 aaagcttcta tctatcgaat tattgtttta ggattaggtt ttgtggtggg agttattttt
    36781 gcatatctgc ttcgttctca atccattatt ttttgtgcag ctatggctgc agccggtctg
    36841 attttttgta aaccatctga aatgaaaatg attgaggaac ttcagctcga caaagaagaa
    36901 aatattcctg ccgaaaaata gttttcccag acaactaaag ttctaaccga aaagatttta
    36961 ttatgattat tgccgttgat tttgatggaa ccatagtaac gcatgaatat cccaaaatag
    37021 ggaaagaaat accttttgcc atcgaaaccc tgaaacgtct tcaagatgaa tttcagcatc
    37081 aaattattct ttggactgtt cgcgaaggca gagaactgga ggaagccata gaattttgta
    37141 aaatgagagg attggaattt tatgccatta ataaaaatta tccggaagaa ggagacgatt
    37201 atgccgagtc gcgcaagata cgtgccgatc tatacattga tgacagaaat ttgggaggtt
    37261 tgccagattg gggcgtgatt tatcaaatga ttgctgccaa tagtgtagcg aatccttttg
    37321 ctttgaatgg agaacaacct gctttcagac aaaccaaaaa aggtttcttt aaatcgattt
    37381 ttggaaaata aattttgatt gaagtatatt tttggagtgc tttataaaat tttcacattt
    37441 agcttactgt catttcaccg caaagactga cacttaactt gtgcttgatt atttctgttg
    37501 aatgatattc tcccagtaaa tgcatgagtt tggctaccag agcttcagtt gtgcaatcgt
    37561 aaccacttat gacacctgca ttcagcaaat ttaagcttgt ttcgtatttg cccatttcta
    37621 cagttcccgt gctgcactga cttttgttta caataataat ccccttttct acggcagctt
    37681 tcaattcttc atataaccag ataaggcgtg gtgcgtttcc agcgccataa ctttcaagaa
    37741 tgacggcttt taattcggga atatttaatg tagcatgaac attttgctgt gtaattccgg
    37801 gaaaaagctt aagaatggca atgttcggat taatgttggt tttgacttcg agtttggaaa
    37861 aagattcggg atagtgaata taggaattaa aatatttgat atgaacgcca acttttgcca
    37921 aagggggata attgtatgaa ttaaaggcgt taaaatgttc ggcgtttttc ttcgtggttc
    37981 gatttcctct gaaaagcgta tcttcaaaaa atatgcagac ttctggcaca atggctttac
    38041 cattttcttt tgcagttgct atttcaatgg cagtcagcaa attttctttg gcatcggaac
    38101 gcatcatgcc aattggtaat tgagcacctg tgaaaatcac cggttttgaa agattttgga
    38161 gcataaaact cagtgcagaa gcagaataag ccattgtatc ggtgccatga agcaccacaa
    38221 agccatcaaa attatcaaag ttctcctcaa tgattaatgc aatttctttc catgtttcgg
    38281 gttgtatatc tgacgaatcc attaaaggtg aaaaggggat acttttgatt tcgattccgg
    38341 tttgtttgag ttcgggaagg tattcctgca ggcgataaaa atctattgga cgcaatgctc
    38401 ctgttgttgg gtcttcagac atgctgatgg ttccgccggt gaatatcaat aagacagtgt
    38461 ttttgccttt cacatttgag tattttttaa tttgcgcaaa gataatgcaa atttcttaac
    38521 gattagcttt ccttttgata tttcttttgg ccaactgaat tttttttaaa taattcattt
    38581 ttagtacatg acaaatattt agacatttgt tttatatatc tgtaaaacaa tacatataag
    38641 ccaagctctt cgggttgatc ttttccggtg agccgaaaaa acaagtgaga aaaacgttta
    38701 aaaatcgtcc ttgtttgagc gacgaaggag cgagtttaga agattttagc caacgaacgt
    38761 caatttttct tgtaaagacg ttgcatgcaa cgtctttact gatgaaaatt acaaacaagt
    38821 aagtggagtt cggggcaaat tcacgttatt aaatatctga aaataaatag tataaatatt
    38881 tgtacaattt ttgtcatgta ctaag
//
LOCUS       DAOTAX010000014        35261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_987_length_35261_cov_33.213884, whole genome shotgun sequence.
ACCESSION   DAOTAX010000014 DAOTAX010000000
VERSION     DAOTAX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 35261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35261
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_987_length_35261_cov_33.213884"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1044
                     /locus_tag="PLM04_02875"
                     /pseudo
     CDS             316..1044
                     /locus_tag="PLM04_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1240..1404)
                     /locus_tag="PLM04_02880"
     CDS             complement(1240..1404)
                     /locus_tag="PLM04_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02054.1"
                     /translation="MNVELSSIIILYALKGLEVPYIRENMGIEFEKNKNSIVDFVLAG
                     IKLPTSKEMI"
     gene            complement(1401..1841)
                     /locus_tag="PLM04_02885"
     CDS             complement(1401..1841)
                     /locus_tag="PLM04_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HON02055.1"
                     /translation="MPEKTSRTKKLLIEVARKLFAERGKKNVTMNDIAETSGKGRRTL
                     YTYFKNKDEIYKAVIDSELIKIFQEIQSVSLEKIEPDIKLTKHIHKHLDAIKETVNRN
                     GSLKADFFRDIYEVERTRRKIDIQEIELIKSILREGIEKNSLNV"
     gene            complement(1897..2850)
                     /locus_tag="PLM04_02890"
     CDS             complement(1897..2850)
                     /locus_tag="PLM04_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HON02056.1"
                     /translation="MNNTIARINTCVNNALPKAFKTIIWLLKIILPISLGVSLLQYWG
                     IIATLSVYLTPLFSLVGLPGESAIVFITSIFMALYAPIAIITTLPLEMREITILALMC
                     LISHNLIVETAVQKKTGSSAFIMFSLRIISSFVTAFVLNKILPMNMGHEKIVQAGVSY
                     TDMGSMLLGWLKSSGWLILKISLIITGLMILQNILKEFNILDMLSKIFAPFMKIMGLP
                     KNCSFLWFVAQILGLTYGSAVMIESVENKEISPHDADLLNYHIAVNHSLLEDTLLFAA
                     IGVPVLWMIIPRFVLAVIILWGVHGIRALRRQFSHPEAEIV"
     gene            complement(3142..6210)
                     /locus_tag="PLM04_02895"
     CDS             complement(3142..6210)
                     /locus_tag="PLM04_02895"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02057.1"
                     /translation="MKKLIVKPLIIAFQIILILLPTDAKAQYFGRNKPSYQTFKYQIK
                     QTPHFEIYHYLTNDTVIKYFAGWTEEWYRFHQLIFKDTFKTRNPLILYANHADFQQTN
                     AISSIISEGTGGVTESLKNRVILPFAPTLYQTDHVLGHELVHAFQYHKLLKPDSAKYY
                     NINNLPLWMVEGMAEYLSIGSVDANTSMWMRDALIQKKFPSINDLNNTSEYFPYRYGQ
                     ALWAFIGKTWGDSIILPLFEKTAQYGLDRAIDSLFHFNEKTLSGMWKTATENHYGKLM
                     KDTTYNVIGKQIISKKNSGSTNVSPSLSPDGKYIAFFSEKNVFTLDLYLADAQNGKII
                     KKLWSVNRNQEIDDFSFIESGGTWSPDSKQFAFVVYSKGRQKLAIVDVRKAKMKLLEI
                     KGLNSFSNPEWSPDGRYIVVTGLVNGIGDLYLYEVKSGKTEKLTHDLFSNIHASWSSD
                     GKKIVYSSEYLCGAGSKKFAFHINILDLETREIKTLDVFPKADNLNPRFSNNNQYIYF
                     VSDADGFRNLYKYDLNTDKVFRLTQYMTGISGITTFSPAISTDRKNDLIAYTYYFNGN
                     YEIYTASATEFNDEEVNGNQVNFDAATLPLLHHLSVNIVDSTLYNRPAVIALPVDSIN
                     NLPYRPKLKLDYISNSVGVGISTGPSYNTTDMAGSIYMIFSDMVGNHQIYSSLSLNGE
                     IYDFGGQVAYLNQGRKIKWGASLSHIPYRSGNMFWTIDTLKINNTDYIFDNLVLDYIR
                     MFEDNISLFSYYPLSQTRRFEGGISASWYSYRIDRYNNYYDALGYYVGGNRKKLPAPD
                     GISFQTANIAYVEDDSYFGMTSPLQGHRARYQIERYFGNINLYNALIDYRRYLFNKPV
                     GLAFRLYHNGLYGKSSQIELASPMYIGYPWLIRGYEQISVYGGANNVAGYSSLNVNNL
                     VGTRMALVNAEIRIPFSGPKVLAPIKSNIFLTDLNLFLDGGLAWNKGDQIKIKWEPSN
                     FNDRIPVWSTGISMRINLFGYLVIEPYYAIPFQNGGFRNKVFGINFVPGW"
     gene            complement(6406..7785)
                     /locus_tag="PLM04_02900"
     CDS             complement(6406..7785)
                     /locus_tag="PLM04_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HON02058.1"
                     /translation="MQRNIIFIVYLLIIIFLTSCREGEKVKLSSENSFRDIQEIVKSD
                     TLRAGTMYGSTSYFLFREEWMGYDYEMVKNFADYLKVNLKVIPAATESELFQLLKEGK
                     IDIAAYNLPVTKALKKHFLYVFPQPQTYMVLVQNMGRKALSSVTDLIGKEVYVKKNTL
                     FYQRLNALNKEIGGGIKIVIAPDSVSNDELIEMVANNKIDYTIAYYDKALLHKSYYKR
                     LDCRLPVSFEQPNGWLIRKENTDLFKTLSTWGQLAGTQRTEDRLINLYWEKSPYFITR
                     KIKIPKGSISPYDEMFKKYAPTIGWDWRMLAAIAFHESKFDSAEISRAGAVGLMQLMP
                     QTAASFGLNHENWFNPEANLDAAVQYIKSLNLIFREIKDKDERIKFILASYNSGPSHV
                     LDAMALAKKYGKNPHIWFNHVETYLLKKTDPDYYRDPVVKYGFFRGAETVRYVQNVLD
                     TYEKYLSRK"
     gene            complement(7796..8533)
                     /locus_tag="PLM04_02905"
     CDS             complement(7796..8533)
                     /locus_tag="PLM04_02905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HON02059.1"
                     /translation="MTNADLQKIMFFDIETVPQTSSFSELSNEMAQLWEEKFQKLRIR
                     MPEKYKEDTSASEAFDTSAGIYSEFGKIVCISVGFIYFQGETMYFRTKSFASDDEKEL
                     LKNFSDLISKFCSSKDNMLCGHNIKEFDIPYICRRLLINGLELPPVFQISGRKPWENN
                     FLDTLELWKFGDYKNYTPLKLLTAVFGIPTPKDDIDGSQVAQVYYKEKDLERIANYCT
                     KDVIATAQVFLRLNGFELISPQNIENL"
     gene            complement(8555..9163)
                     /gene="udk"
                     /locus_tag="PLM04_02910"
     CDS             complement(8555..9163)
                     /gene="udk"
                     /locus_tag="PLM04_02910"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HON02060.1"
                     /translation="MLTIGIAGGTGSGKTTVVRKIIERLPAGEVVVLPQDSYYRDSSF
                     LPLEKRLEINFDHPDSIEFELMIKHLQDLKEGKPIEQPIYSYLTCTRAAETVTIYPRE
                     VIIVEGILVLCNPELRELMDLKVFVDADPDDRLIRVINRDIEERGRTVNKVIERYEHT
                     VKPMHELFIEPTKRFADLIIPQGGDNHIAIDILTKYIENNLK"
     gene            complement(9380..10483)
                     /locus_tag="PLM04_02915"
     CDS             complement(9380..10483)
                     /locus_tag="PLM04_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HON02061.1"
                     /translation="MILKKITKWGLISANIIVACALLFSVFAGFVSPAVFTFFAYFAL
                     VFPIIFFLNFAFFLLWIVLKKWYFLISLSLMLVSFPQLKTNFSFHLKKQPVSSIQPDF
                     TIISYNTHMMGLEKKHDEANKKINPVIQYVLSTDADIVCLQEFMVSTNEKYLTEEDAM
                     KAFSKYPYHHIYFKAHEKKSLLGIATFSKFPIINRQTIPYNSSANASIYSDINIHGKT
                     IRVINNHLESNQLTEKDKAMPYQLKDNFNTDLATNITVYFSKKLGAAYKLRAIQADEV
                     KKVISSSPHPVVVCGDFNDVPSSYVYTKIRGKLKDCFLEQGKGFGWTFNEKHYRFRID
                     YIFHDPSLVLLNFKKDKVPYSDHFPLKAAFQKL"
     gene            complement(10550..11443)
                     /locus_tag="PLM04_02920"
     CDS             complement(10550..11443)
                     /locus_tag="PLM04_02920"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HON02062.1"
                     /translation="MNIFDNIKLSYRQGSHLIKLIYINLGVFLLYHAVLITFKLFNAS
                     GIFLLPFLAMPADGRQLLYHFWTPVTYMFMHEGFLHLFFNMLCLYWFGKIFLMYYSEK
                     QLTGLYLVGGWVAGLFYFLSFNIFPYFDAAVSQGLLLGASGSIMAIIVASAVASPNLE
                     MRILFLGGIKLKYIALVAILTSFFGITSNNAGGEIAHLGGALMGYLFVVSLRKGKDIT
                     KWLNGVLDNIVNLFSRKKLKIKKPKYSTSYRKMTDEEFNMEKSRRMQEIDRILDKIKT
                     SGYDSLTAEEKKRLFEQGKPR"
     gene            complement(11520..12308)
                     /locus_tag="PLM04_02925"
     CDS             complement(11520..12308)
                     /locus_tag="PLM04_02925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HON02063.1"
                     /translation="MSYRSSFFGSIPVVVKNLILINIILWLATLVLPRFFLRWGINVD
                     LVNILGMHYWGSKQFNPAQLITYMFLHGNFEHIFFNMFALFMFGTVLEQFWGPKRFML
                     YYMVTGIGAALIQQLFWSVEYQPVISALNEAINTGNKDALFSHEGVLSRFFRINNLAA
                     LDTTSLIELKHLFVNLPVTIGASGAIFGLLLAFGWLFPDAKLMLIFLPIPIRARIFVL
                     MYAVAELFLGVAGFTGDNVAHFAHLGGMLFGIFLILYWKKKGVH"
     gene            12601..12873
                     /locus_tag="PLM04_02930"
     CDS             12601..12873
                     /locus_tag="PLM04_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HON02064.1"
                     /translation="MNKAELISAVAEKSGLSKADAKKAIDAVVKSISEALIAGDKVSL
                     VGFGTFSVAERASRSGINPATKKPITIPAKKVAKFKAGAELSDAIN"
     gene            complement(12970..13515)
                     /locus_tag="PLM04_02935"
     CDS             complement(12970..13515)
                     /locus_tag="PLM04_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HON02065.1"
                     /translation="MKAMVFLADGFEEIEAIAPIDIMRRAGIEVTTISVSREKEVRGA
                     HGVLVEADRLFEEVSFSDADLLFLPGGMPGTKNLNAHDGLKQLLLKHANEGKKLAAIC
                     AAPSILGELGLLKGKEAVCFPGFEETLKGAIVSDKKVVQSGNIITGKAAGAAIEFALK
                     LVENLKGKSAAEQVGLSIFAF"
     gene            complement(13594..14286)
                     /locus_tag="PLM04_02940"
     CDS             complement(13594..14286)
                     /locus_tag="PLM04_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HON02066.1"
                     /translation="MKKSELYGIVGSIIFCMMLLVLLLIIKMHASTSVEPEGIMVSFG
                     ETLEGGSRAASPSAAEEYAVSKKASEKILTQKDPSVSLDKKTDNREQERELSEKKRLE
                     QEAISKANIMEGIFDKKISGNENVGDENLLKGNPAGRGTSNGNSWSLSGRNLVGNLIT
                     PRYEQNVEGEVTVNIRVDENGVVEGVSIGVPTNISDATILKAAMEAAKNTRFSPGKGK
                     ATGTITYHFRLK"
     gene            complement(14293..14709)
                     /locus_tag="PLM04_02945"
     CDS             complement(14293..14709)
                     /locus_tag="PLM04_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HON02067.1"
                     /translation="MALKKRNKVEAGFAMSSMTDIIFLLLLFFVIASTLSSPNDIKLN
                     LPESRSKSSTKQIIAKVAIDAEGKLSVAKGNQKPVPIEPKLLESYLLNMASQDSISFV
                     ALYADQNIAYKEIVRVLDIANQHGFKIVIATKPFEN"
     gene            complement(14731..15423)
                     /locus_tag="PLM04_02950"
     CDS             complement(14731..15423)
                     /locus_tag="PLM04_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HON02068.1"
                     /translation="MINLLILQASANVATTVADPLQSEINLWTMAKFGGPIMIILALM
                     LAFAVYLFIERLITINQASKEDAFFMERIKDYIYEGKIETALKLCRDTNSPSARMIEK
                     GITRLGHPLNDVLVAIENVGNMEIAKLEKGLVIMASISGGAPMLGFLGTVTGMVSTFF
                     NLSQKAGGNILLGDLSVGMYQAMVTTIGGLIVGILAYFAYNYLVSRVDSVVNILESKT
                     MEFLDLLNEPVK"
     gene            complement(15474..16187)
                     /locus_tag="PLM04_02955"
     CDS             complement(15474..16187)
                     /locus_tag="PLM04_02955"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HON02069.1"
                     /translation="MTKLSVNINKIATLRNARGGNTPDVCKAAIDCEKFGADGITVHP
                     RPDERHIRYSDVWALKPILQTEFNIEGYPSPNFMKLVTMVKPTQVTLVPDPPEAITSN
                     AGWDTEKHLDFLKDITAELQDAGIRVSIFVDANIKSVEYAALAGADRVELYTEPYARE
                     YAENREKAVATFIEAAKAAKKLGLGLNAGHDLNLQNLHYLHACIPWLDEVSIGHALIC
                     DALYLGLQETIKQYKNQLK"
     gene            16601..17473
                     /locus_tag="PLM04_02960"
     CDS             16601..17473
                     /locus_tag="PLM04_02960"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HON02070.1"
                     /translation="MKVAIFGNIYRPVIINSIKVLFDFFSEKQIPLMLDSDLYDFLVH
                     IGGYRLENVEKIENDNFKADVALSIGGDGTFLNTAARIGDKEIPIIGINTGRLGFLTD
                     VSNDEIIEALEALLEGKFRIEERTLLLVKTSDGTPLQYPYALNEVSVLKQDSSSMISI
                     NASVNGELIHTYQADGLLVSTPTGSTAYSMSVGGPIVVPQAQNFILSPVASHSLNVRP
                     LIIPDNWNIDLEVHSRTHSYLVALDGRSKVLEQTTQLHIAKAPFTIKVIKQLQHTFFD
                     TLKKKLMWGVDKRN"
     gene            complement(17543..19726)
                     /gene="recQ"
                     /locus_tag="PLM04_02965"
     CDS             complement(17543..19726)
                     /gene="recQ"
                     /locus_tag="PLM04_02965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HON02071.1"
                     /translation="MLKKVSLAKELKKYFGFDTFKGNQEQIIQNVLNGNDTFVLMPTG
                     GGKSLCYQLPSLLMDGTAIVISPLIALMKNQVDAMRNFSEEDGVAHFINSSLSKQAIE
                     QVKCDILSGKTKLLYVAPESLTKEENIEFLKHIKISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIVNEIGIAPIIALTATATPKVQHDIQKNLGMLDAKVFKSSFNRPNLYYEVRQKT
                     NNIDKDIIKFVRSQEGKSGIIYCLSRKKVEELAEMLHVNGISALPYHAGMDAAQRSEN
                     QDKFLMEEASVIVATIAFGMGIDKPDVRYVIHYDMPKSLEGYYQETGRSGRDGGEGQC
                     IAFYSYKDLEKLRKFMQGKPLAEQEIGNQLLIETQAYAESSICRRKLLLHYFGEEYKE
                     DNCGNCDNCMKPQKFMEAQELLTLVLETISILKEKFKAEHIVDILKGNETSDVVSYQH
                     NELENFGAASDEDEKLLQAVIRQALISGYITKDVETYGVLKITPKGKDYLKNPTSFPI
                     VKDNDFEEDEEVNLKNIASTSAADEVLFSILKDLRKKLSKKLDVPPFVIFQDPSLEAM
                     ATTYPITMDELQNIPGVGAGKAKRYGDEFLKVIKEYVKENDIIRPEDLRVRTVANKSK
                     LKVSIIQAIDRKIALDDIALSKGLDITELLDEIEAIVYSGTKINIDYFLEDVMDADRV
                     DEIFEYFNSAETDSIDDALDKLGIDDFSETEIRLVRIKFLSEVGN"
     gene            complement(19988..20347)
                     /locus_tag="PLM04_02970"
     CDS             complement(19988..20347)
                     /locus_tag="PLM04_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON02072.1"
                     /translation="MSTYEQLEALARYILPKEILDDFDIVGIEEKSGVLNSIFLKDNN
                     KIKYLSNVAESQFKSFNTIAATVYEHHEEVLNFFINRSTNASAESFNAKIKSFRASLR
                     GVIDIKFFLFRLTNIYA"
     gene            20472..21740
                     /gene="purD"
                     /locus_tag="PLM04_02975"
     CDS             20472..21740
                     /gene="purD"
                     /locus_tag="PLM04_02975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HON02073.1"
                     /translation="MNILLLGSGGREHALAWKIAQSSQLTNLYIAPGNPGTSTLGTNL
                     PFSADDFQSIKIAVLEHEIDWVIVGSEDPLVKGIADFFASDKQLKNVSVIGPSKIAAQ
                     LEGSKNFAKKFMLRHGIPTAKYFSVTSETIDEGIQFLQQLSSPYVLKADGLAAGKGVL
                     IIDSLEEAEQELKSMLEGKFGTASKTVVIEQFLSGIECSVFVITDGNNYKILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVSFADKYFMKKVEQNIIRPTINGLREEGIVYKGFIFFG
                     LINVKGQPYVIEYNVRMGDPETEAVMLRIKSDFVDLLGGVVNENLDEKEIKIDERFAA
                     TVMLVSGGYPQAYEKGKEISGLDKVEGSIIFHAGTALKNGKLVTNGGRVMAISSYGNT
                     MKEALEISLRNAEKINFEGKYFRKDIGFDL"
     gene            21780..22466
                     /gene="cmk"
                     /locus_tag="PLM04_02980"
     CDS             21780..22466
                     /gene="cmk"
                     /locus_tag="PLM04_02980"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HON02074.1"
                     /translation="MKKIVVAIDGFSSGGKSTMAKDLARDVGYTYIDSGAMYRAVALY
                     AMRNGWITENDIDIEALKKNIGSLKIDFKTNEHGVSETYLNGENVEKEIRTLEVANAA
                     SRVSALDFVRRELVRQQQQMGLQKGIVMDGRDIGTVVFPEAELKIFLTASPEIRAQRR
                     MEEMLARGEKVDFQKVLANIKERDERDLHRSESPLKKAPDAIVIDNSNLTRNEQRELL
                     KTLFYQKTLE"
     gene            22532..23398
                     /locus_tag="PLM04_02985"
     CDS             22532..23398
                     /locus_tag="PLM04_02985"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HON02075.1"
                     /translation="MLNIEIDNKSGFCFGVVKAIEKAEEELEKGSTLYCLGDIVHNEE
                     EVERLQRLGLVTVNHEQYNQLHNVKVLIRAHGEPPSTYETARKNNITLIDATCPVVLK
                     LQSRIKKNYEELASEDGQIVIYGQTGHAEVKGLVGQTDGKAIVIEEEKDLDKIDFSKK
                     VHLFSQTTKDIEHYHHLIEEISKRMVKKDDFKFSDTICRQVANRIPNIINFAQQNDVI
                     LFVGGKQSSNGKVLYEHCKSINPQTYYISDCKQVDELEIQKDKKIGICGATSTPKWLM
                     DCIAKRLEERLA"
     gene            23442..24425
                     /gene="pfkA"
                     /locus_tag="PLM04_02990"
     CDS             23442..24425
                     /gene="pfkA"
                     /locus_tag="PLM04_02990"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HON02076.1"
                     /translation="MKYEIKCIGLLTSGGDAPGMNAAIRAVTRTAIFNGIRVKAIYRG
                     YKGLITGEIKEFKTENVSNIIQQGGTIIKTARCAEFMTPEGRKMAYETMRKEEIDALI
                     VIGGDGTLTGARIFAQEFNVPIVGLPGTIDNDLWGTDVTIGYDTALNTIIDAVDKIRD
                     TASSHERLFFIEVMGRDAGFLALSSALASGAEAAIIPERETKVDQLEELIKNGFRKSK
                     NSSIVIVAESEITGGANGLAERMRREHPEYDVRVTILGHIQRGGSPTASDRILASRMG
                     VAAVDALLDEQRSIMIGIVNDEIVHVPFTKAIKFDKPVNEDLLGVIQILSI"
     gene            complement(24567..25958)
                     /locus_tag="PLM04_02995"
     CDS             complement(24567..25958)
                     /locus_tag="PLM04_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HON02077.1"
                     /translation="MKNKYIDLIEQTFEFPNDEFNVIDNELWWCGVPLMDVIKQYGTP
                     LRIAYLPKIAENIQKARRMFNVAMAKVDYQGDYMYCYCTKSSHFSFVMEEVLKHDVHL
                     ETSSAFDINIMESLYEQGLLKPETYIICNGFKRPQYIENIQNLIRLGFSNVVPILDNK
                     FELDLLLKDFDKKLKIGIRIAAEEEPKFDFYTSRLGIRYNDIIPYYTKHIANNPQVEL
                     KILHFFINTGVRDTAYYWNELSKAVNLYCELKKKCPELDSLNIGGGFPIQTHLDMSYD
                     YEYMAEEIIAQIKNICTQNGVPEPNIFTEFGSYTVGESGAMLYSIVNQKKQNDRELWD
                     MIDSSFMTTLPDTWAINQRYVFLAINNWDSEYERVNLGGLTCDSEDFYNAEVHSNAIF
                     LPKMEEGREQYIGFFHTGAYQESLSGYGGIQHCLIPAPKMVIIDKDEDGEYFTKLFAK
                     EQSYKSMLKILGY"
     gene            complement(26474..28939)
                     /gene="priA"
                     /locus_tag="PLM04_03000"
     CDS             complement(26474..28939)
                     /gene="priA"
                     /locus_tag="PLM04_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HON02078.1"
                     /translation="MKYIEVILPLPLPNTFTYAVPEADENSIQQGMRVVVPFGKKKLY
                     SGIVYYVHILRPETTYQIKEIVGILDEEPILRRPQIKFWEWIANYYQAHLGEVYQSAL
                     PAGLKLESETLVRVKPDFDTNVHFSEREKKILDVLLNQKKPISLSALAKDSGISNVLP
                     QLKLLLEKGVIEISEQLTEKFHPKTETYIRLRPEIQQDEKLHEIFDQLKSAKKQLSLL
                     MNYIDLSKCLDPKNVREVSRKTLLEKSGISPAVLKALTDKKIFETYEKIVGRLDFSEN
                     ELQKPSILNEFQTKALQEIEKKFKEKPVVLLHGVTSSGKTELYIHLIQKVLNEGKQAL
                     YLVPEIALTTQLTARLKRIFGKKLGVYHSKFSDAERTEIWNNLLNDKGYEVIIGVRSS
                     IFLPFRQLGLVIVDEEYETSYKQFDPAPRYHARNAAIVLASMHGAHTLLGSATPAIES
                     YFNAVTGKYGLVKLDRRFEEISLPEIVVVDLKEAYHKKQMNGHFSDLLMHEMQKTLQN
                     KEQIILFQNRRGYAPYLECKSCNYIPKCKNCDVSLTYHKTTNALTCHYCGYTESYPAS
                     CPVCGTPAAFSTHGFGTEKIEDEIKKIFPEVRISRMDLDTTRSRKSYETIITDFEEYK
                     IDILIGTQMITKGLDFDKVSLVGILNADNILNFPDFRSHERAFQLLSQVSGRAGRKDK
                     QGKVILQTYQPKHPIIQQVMTNDYEGMFANQCEERKQFKYPPYTRLILLIVRHHDRQI
                     LQEASHQLTNSLRSVFGRRVQGPSDPLIPKIQNFYIKHILLKIEIEASPEKTKKLIRD
                     CINLSLSQPSFKSVHLQIDVDPL"
     gene            complement(29042..30319)
                     /locus_tag="PLM04_03005"
     CDS             complement(29042..30319)
                     /locus_tag="PLM04_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HON02079.1"
                     /translation="MKTHTKKFYFPNFEKIICLFLLLTGFFSKTLSQDFAKGADVSWV
                     TQMEAAGKKFYTAEGVETECMTLLKSLGMNTIRLRVWVNPADGWCNAQDVLLKAQRAN
                     DLGMRIMIDFHYSDSWADPGKQTKPAAWSNLSFADLKTALSQHTNEVLTFLKNNDITP
                     EWVQVGNETSDGMLWEDGRISKNPQNYAELNNAGYDAVKSVFPNAKVIVHLPNGYSNS
                     LYRWFFDTLKNYGGKWDVIGMSVYPFWYTTKNDWRNCNISCFNNMNDMVSRYGTEVMI
                     VECGMQWDAPEACRDFLSDLINKTKNVSQGKGLGVLYWEPQCYPGWNGYTLGAFDNTG
                     KPTIALDAFKEVSQSTHPLQLKTGWRYDDLQKVILFDKTFSKVTLFDSAGTAIQSFAS
                     TNRISMKNMQKGIYILHAALKNTEPLLIKFIYN"
     gene            30604..31323
                     /locus_tag="PLM04_03010"
     CDS             30604..31323
                     /locus_tag="PLM04_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HON02080.1"
                     /translation="MKSKYFVNILIMTMLCFYMQQLNAQDWPNLGRFRAENEKLGAPD
                     KGEKRVVFLGNSITEGWINIRPDFFKNRSYINRGISGQTTPQMLLRFRQDVIKLQPAV
                     VVILAGTNDIAGNTGPSTLEMIEDNLSSMSELARVHNIKVVLSSVLPAYDYPWRRGLQ
                     PAEKIVTLNAWIKDYCQKNGFVYLDYFSAMADERNGLKSEFSEDGVHPNQKGYQVMEP
                     LAEAAIQKALKQNFKKCPCNK"
     gene            31441..33726
                     /gene="rpsA"
                     /locus_tag="PLM04_03015"
     CDS             31441..33726
                     /gene="rpsA"
                     /locus_tag="PLM04_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HON02081.1"
                     /translation="MEENQINLEKNESALTENVSPEAVEAKKEETQEVNEVLSPEEES
                     VAESHAAEPIVAETPEVSVPEEPVKESVAPVQAEITPEEFDWDAYEKGNTLTTEAKKE
                     LENVYDKTLNAVKDKEVVEGTVISINKREVVVNIGYKSDGVVPMSEFRYNPDLKVGDK
                     VEVYIESQEDKKGQLLLSHKQARISRAWDRVNAALESQEVVKGFIKCRTKGGMIVDVF
                     GIEAFLPGSQIDVKPIRDYDAFVNKTMEFKVVKINQEFRNVVVSHKALIEEELEQQKK
                     EIISKLQKGQILEGVVKNITSYGVFIDLGGVDGLIHITDLSWGRINHPSEVVSLDEKI
                     NVVILDFDEEKKRIALGLKQLTPHPWDALDPNLKVGDKVKGKVVVITDYGAFVEIAPG
                     VEGLIHVSEMSWSQHLRSAQDFLKVGDEVEAIILTLDRDERKMSLGIKQLKSDPWENI
                     EERYPVGSKHVAKVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPSEFTQIGSEI
                     EVVVLDIDKENRRLSLGHKQLEENPWDALESVFAVDSIHDGVVVEMLDKGAVIALPYG
                     VEGFASAKQLVKEDGTQAQVDEKLPFKVIEFNKDAKKIVLSHTRIHEDLKRAAKTESA
                     EGETSTSPKKSVTKRTKKEESSTVQTQAIEKTTFGDIQSLVDLKDQMESEAAKKLAKK
                     ETKSKKAEPAEKEEPAEVKMAEETKVEEPSEKPAETEEKSEKPQKSSKKEKTEKVEEK
                     VVEAEETPASEEAETSEKPIE"
     gene            complement(33818..35044)
                     /locus_tag="PLM04_03020"
     CDS             complement(33818..35044)
                     /locus_tag="PLM04_03020"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HON02082.1"
                     /translation="MKKIKVEQPVVELDGDEMTRIIWRIIKEQLILPYLDIDIKYYDL
                     SIQNRDLTNDEVTIEAAHAIKKYNVGIKCATITPDEARVKEFGLKKMWKSPNGTIRNI
                     IGGTVFREPIICSNIPRLVPNWTKPIVIGRHAFGDQYKATDVLIKGKGTLKMTFTAEN
                     GETQEWEVYNFQGDGVAMSMYNTDESIYGFARSSFQAALDKKWPLYLSTKNTILKTYD
                     GRFKDIFQEVYEKEFREAFQNAGITYEHRLIDDMVASVLKWNGGFVWACKNYDGDVQS
                     DSIAQGFGSLGLMSSVLITPDGKTIEAEAAHGTVTRHYRLYQQGKETSTNPIASIFAW
                     TRGLAHRGKLDGNNALIDFAKKLEEVCVQTVESGKMTKDLAILVHGEKVSRTDYLSTE
                     EFLTALKQNLEAKLSA"
     gene            complement(35104..>35261)
                     /locus_tag="PLM04_03025"
     CDS             complement(35104..>35261)
                     /locus_tag="PLM04_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HON02083.1"
                     /translation="KEVYTPIFAMARIVGWCAHRIEELNFTSKRIIRPAYKNVNEIQQ
                     FIPLEKR"
BASE COUNT        10901 a         6465 c         6722 g        11173 t
ORIGIN      
        1 ttacaaaaac agaaaaatat gctaccggac aaaattaagg aaaaaatttc tgaaattgga
       61 acagatttca atgaaaccca catgtattgc atacacaaac acgaatgaat acaatattca
      121 gacatgtggg ttccatcaga cctttaaaaa ataaattata ttctgatgaa aacattttat
      181 tcttttcagc ccttattttt gctgcttttg ttggtggttt gtccttttgt tggtgcacag
      241 gataaagaaa tttccactcc aacatcacct tttactattg atctctggca aaacggatta
      301 ccaaatacca acggaatgga aacattgggt tacgatgatg ccaaatcgaa cttcaaaccc
      361 tgcattaccg tttatcttcc caaagcttcg caaccaacaa aggctgtagt tatttgcccc
      421 ggaggtggtt atcagcattt agccatgaat catgaaggtt acgattgggc ttcgtttttc
      481 aacaatcagg gaatagcggt tattgtttta aaataccgca tgcctaacgg acatttggaa
      541 gtgccaattt ccgatgctta tgaagccatc cgactaacta aaacgaatgc caaaacttgg
      601 aatattcatc ccgattgtat tgggataatg ggttcttctg ccggaggtca tttggctgct
      661 acagtggcaa ctcatgcgcc tacagaatta cggcctgatt ttcaaatttt gttttatccg
      721 gttatcacca tgaacaaaca agaaacacat gccggttcgc gcgaacatct gctaggaaaa
      781 aatccaacat ttgaaatgga aaaattatac agtaacgaat tgcaggtcaa tgccaacaca
      841 ccgcgagctt ttatcgtatt gtcggatgac gacgatgtgg taaaacctgt gaattctgta
      901 aaatactatt tggcattgca acagcaaaac attcctgcaa ccatgcatat ttattccgac
      961 ggcggtcatg gctggggaat cagagaatct ttcaaatacc atttggaaat gttgacagaa
     1021 ttgaagtcgt ggttgggaac attttgaaat gaatacaact ttcaaattta atgaataaaa
     1081 aactaacact aaaaatcatc aggcagtagt gattttttac cacaaagcat ccggcaaaaa
     1141 acagtgtttt ccatataatc ttttgttatc agcggaggag tgttctattt tgtgggtaag
     1201 tatggtatct taatagcgta ctttcttatt taatccaaat taaatcatct cctttgaagt
     1261 cggtagttta attccggcca gtacaaagtc aacgatgctg tttttgtttt tttcgaattc
     1321 gatacccata ttctctctga tgtagggaac ctccagccct ttcaaagcat ataagataat
     1381 gatagaggac aattcgacgt tcatacgttt aaagagtttt tttcaattcc ttctctcagg
     1441 atggatttaa tgagttcaat ttcttgaatg tcaattttac gccgggtgcg ttctacttca
     1501 taaatatcgc gaaaaaaatc ggctttgagc gaaccatttc gatttacggt ttcttttatg
     1561 gcatctaaat gtttgtgtat gtgtttggta agtttaatgt ccggttcgat tttttctaat
     1621 gaaacagatt gaatttcctg aaaaattttg atcagttcgc tgtcaattac ggctttatat
     1681 atttcatctt tgtttttgaa ataagtatat aaagttcttc ttcctttccc tgaagtttcg
     1741 gcaatgtcat tcatggtaac attttttttc cctctttcag cgaataattt tcgtgcaact
     1801 tctatcaata atttttttgt tctggatgtt ttttcgggca taaaaaatat gtttgaaaca
     1861 gggaaaattt ttctcagctc ggttttttat tgattgtcaa acaatttctg cttcaggatg
     1921 agaaaattgg cggcgcaatg ctctgatgcc atgcacaccc cataaaatga taactgccaa
     1981 aacgaaacgg ggaattatca tccacaatac aggaacccca atagcagcaa aaagtaaggt
     2041 atcttccagc aaggaatggt ttactgcaat atgataattc aacaaatcgg catcatgagg
     2101 gctgatttct ttgttctcga cactttctat catgacagcc gaaccataag ttaatcctaa
     2161 aatttgggct acaaaccata aaaaagaaca gtttttcggt aatcccatta ttttcatgaa
     2221 tggagcaaat attttcgaca gcatgtcaag tatgttgaat tctttcaaaa tgttttgaag
     2281 aatcatcaat ccggtaataa tgagagaaat ctttaagatc agccaaccgg aacttttcag
     2341 ccaccccaaa agcatacttc ccatatcggt atagcttacg cctgcttgta caattttttc
     2401 gtgtcccata ttcatgggaa gtattttgtt cagaacaaaa gctgtaacaa aagaagaaat
     2461 aatacgcagc gagaacatga taaatgcaga agaaccggtt ttcttttgaa cggcagtttc
     2521 tacaatcaag ttatgagaaa tcagacacat gagagccaag atggtgattt cacgcatttc
     2581 cagtggcaat gttgtgataa tggctatggg agcgtataat gccataaaaa tactggtaat
     2641 gaaaactatg gctgattcac cgggaagccc tacaagtgaa aacaatggag taaggtaaac
     2701 ggataaggtg gcaataattc cccaatactg cagtaaactg actcccaaac tgattggtaa
     2761 gataattttt aaaagccata taatggtttt gaaagctttt gggagtgcat tatttacgca
     2821 tgtatttatt cgggcaatgg tgttattcat cgaagtaaag taaaaaaaat tgcaaagaat
     2881 ggaagttata aaaacaagaa aggttgtctc acgacaaccc aatcttttta attaacctta
     2941 aatctaatac catgaaaaac atgtgcaaat gtacatcgat tttttatgtt gtacaacata
     3001 ttcggagaaa aaaattgata tttaacaaca tttaacttat tgatgcatcg tcttgctttt
     3061 tcgactactg aaaatatgag acttttcgtt ttgattctgt attaacaaaa agatattcaa
     3121 gaaattaaat tagttttaaa attaccatcc gggtacgaaa ttgattccga aaactttatt
     3181 tctgaaacct ccattttgga atggtatggc gtagtaaggt tctattacca aatagccaaa
     3241 caaattgatt ctcatggaaa ttcctgtact ccacacagga atacgatcat tgaaattgga
     3301 tggttcccat ttgattttta tctgatctcc tttgttccat gccaatccac catccaaaaa
     3361 cagattcaga tcggtaagaa aaatatttga tttaatggga gccagtactt tcggtccact
     3421 gaaaggaata cgaatttcag cgttgacaag tgccatgcgt gttcccacaa ggttattgac
     3481 attcaaagaa gaatagcctg caacattgtt tgctcctcca taaaccgata tttgttcgta
     3541 tcctctgatt aaccacgggt atccaatata cattggtgaa gccagttcaa tctgagaaga
     3601 tttaccgtac aaaccgttgt gataaagtcg aaatgcgagc ccaacaggtt tgttaaacaa
     3661 atatcttctg taatcaatca aagcattgta taaattgatg tttccaaaat atctttctat
     3721 ttggtatctt gctctatgtc cctgcaaggg cgaggtcatg ccgaagtagg aatcatcttc
     3781 cacataagca atatttgctg tttgaaaact tatgccatcc ggagcaggaa gctttttacg
     3841 atttcctccc acatagtagc ccaacgcatc ataataattg ttgtatctgt cgatgcggta
     3901 tgagtaccac gatgcagaaa ttcctccttc aaatctacgt gtttgagaga gtggataata
     3961 actgaacagc gaaatattgt cttcaaacat gcggatgtaa tccaaaacga gattgtcgaa
     4021 aatatagtcg gtattattta tcttcagcgt atcaattgtc caaaacatgt tgccgcttct
     4081 gtagggaata tgcgataagg atgctcccca tttgattttt cttccctgat tcagataagc
     4141 tacctgaccg ccgaaatcat aaatttctcc attcagcgac aaggaagaat aaatttgatg
     4201 gttgccaacc atatcactga aaatcatgta tatgctaccg gccatatcgg tggtgttgta
     4261 acttggacct gttgaaattc caaccccgac cgaatttgaa atataatcga gttttaattt
     4321 gggtcgatag ggcagattat tgattgaatc gacaggaagt gcaatcactg ccgggcgatt
     4381 gtaaagcgta gaatcaacaa tattgacgga taaatgatgc aacaaaggca aagttgcggc
     4441 atcgaaattt acttgatttc cgtttacttc ttcgtcgtta aattcggttg cggaagctgt
     4501 ataaatttcg taattgccgt tgaaatagta agtataggca atcaagtcat tttttctgtc
     4561 tgtactgatt gctggcgaga atgtagttat tccgctaatt cctgtcatgt attgcgttaa
     4621 acgaaaaact ttatcggtgt tcaaatcata tttatacaga tttctgaatc catcagcatc
     4681 ggaaacaaaa taaatatatt ggttattgtt tgaaaagcga ggattcaggt tatcggcttt
     4741 tgggaaaaca tctaaggttt ttatttctcg ggtttccaag tcgagaatgt ttatatgaaa
     4801 tgcaaatttt ttacttccgg caccacataa gtattcggaa gaatatacta tttttttgcc
     4861 atccgaagac catgaagcat gaatattgga aaacaaatcg tgagtcagtt tttcggtttt
     4921 tcctgatttt acttcgtaaa gatataaatc cccaatcccg tttaccaaac ccgtaacgac
     4981 aatatatctg ccgtcaggcg accattcggg atttgaaaat gaatttaatc cttttatttc
     5041 caaaagtttc attttggctt tgcgtacatc tacaatagcc agtttttgac ggcctttgct
     5101 gtaaacaaca aaagcaaatt gtttgctgtc gggcgaccaa gttcctcccg attctataaa
     5161 actgaaatca tctatttcct gatttcggtt gacactccat aatttcttga ttatttttcc
     5221 attttgcgca tcagccaaat ataaatcaag cgtaaaaaca tttttttcgg agaagaatgc
     5281 gatatattta ccatccggac ttaaagatgg cgaaacattg gtagaacctg aatttttttt
     5341 ggaaattatt tgcttcccga taacattata cgtcgtatct ttcattaatt ttccgtaatg
     5401 attctccgta gctgttttcc acatgccgga aagggttttt tcgttaaagt ggaaaaggct
     5461 gtcaatcgct ctatccagtc cgtattgggc ggttttttca aataacggca gaattatcga
     5521 atcgccccat gttttaccta tgaatgccca taaagcctgt ccatagcgat aaggaaaata
     5581 ttcggaagta ttgttcaaat cgttaatgga tggaaatttt ttctgaatca gtgcatctct
     5641 catccacatg gaagtgttgg catcaacgct cccaatggaa agatattctg ccattccttc
     5701 taccatccac aggggaagat tgtttatgtt gtaatacttg gccgaatctg gctttaacag
     5761 tttgtggtat tgaaaagcgt gaaccaattc gtgtcccagc acatggtcgg tttggtataa
     5821 ggtaggagca aaaggcagaa taacgcgatt tttcagactt tcggttacgc cgcccgttcc
     5881 ttcgctgata atgctgctga tagcatttgt ttgttgaaaa tcggcatgat tggcatacaa
     5941 aatcagagga tttctggttt taaaagtatc tttgaatatc aattgatgaa accgatacca
     6001 ttcttcagtc caacctgcga aatatttgat taccgtatcg ttagtaaggt aatgatagat
     6061 ttcaaaatgc ggagtctgct ttatttgata cttaaaagtc tgatagcttg gtttatttcg
     6121 tccaaaatac tgggcttttg cgtcagtcgg taaaagtatt aaaattatct ggaacgcaat
     6181 tatgagcggt ttaacaatta attttttcat aattcaactt tctgtggttc tcaattcaat
     6241 tgactatgaa aaacaaacaa tcaataaaac aaatctgaaa tcatgtcgct tttgtctcgg
     6301 gtaaaaagaa caaaaagttt taaacaaaat aaatgccaga atgaaaaatt gtagaaaatt
     6361 cttgtaaaac aatggaataa aaaggaacaa ctatgaaaaa atgtcttatt ttctgcttaa
     6421 gtatttctca taagtgtcca atacattttg tacataccga acggtttccg caccgcgaaa
     6481 aaagccatat tttacaaccg gatcgcgata gtaatcggga tcagtctttt ttaggagata
     6541 agtttcaaca tggttgaacc agatatgggg atttttacca tattttttgg ccagagccat
     6601 tgcgtcgaga acatgcgaag gtcctgaatt gtagctggcc aaaataaatt ttattcgttc
     6661 atctttatct tttatttctc tgaaaatcaa atttaaactt ttaatatatt gtactgctgc
     6721 atccaaattt gcttcgggat tgaaccagtt ttcatgatta agaccaaaag aggctgctgt
     6781 ttgaggcatg agctgcatca accctaccgc tcctgccctt gatatttctg ccgaatcaaa
     6841 cttggattca tgaaaagcta ttgctgctag cattcgccaa tcccatccta tagttggggc
     6901 atatttttta aacatttcgt catagggaga aatggaccct ttgggtattt ttattttacg
     6961 ggtaataaaa tacggacttt tttcccagta taagttgata agtcgatctt cggttctttg
     7021 tgttccagcc agttgacccc atgttgaaag ggttttgaac aaatcagtgt tttcttttct
     7081 aattaaccaa ccgttgggtt gctcaaaact cacgggtaaa cggcaatcga gacgcttata
     7141 gtatgatttg tgcaacaaag ctttgtcata gtaagcaatg gtataatcaa ttttattgtt
     7201 ggccaccatt tctatcaatt catcattact aaccgaatcg ggagcgataa ctatttttat
     7261 tccaccgcca atttctttgt ttaaggcatt cagtctttga taaaaaagtg tattcttttt
     7321 tacataaact tcttttccta tcaaatccgt tacacttgaa agcgctttcc gccccatatt
     7381 ttgaaccaat accatatacg tctgaggttg aggaaaaacg tagagaaagt gcttttttaa
     7441 agctttggtt acaggaaggt tatatgcagc aatatctatt tttccttctt taagcagttg
     7501 aaatagttcg gactcggtag ctgccggaat cacctttaaa ttaactttca ggtagtcggc
     7561 aaaatttttc accatttcgt agtcataacc catccattct tctctgaaaa ggaaatagga
     7621 tgttgagcca tacatggttc cggctcttaa tgtatcggat ttgacaattt cttgaatatc
     7681 tctgaaagaa ttttcggaag ataattttac cttttcccct tctcgacagg atgttaagaa
     7741 tataataatc aggagataaa cgatgaatat tatatttcgt tgcattatcg aaatgttata
     7801 gattttctat attttggggt gaaatcaatt caaatccgtt taagcgcaaa aatacctgtg
     7861 cggtagcaat gacgtctttg gtacaataat tggcaatgcg ttcaagatct ttttccttgt
     7921 aataaacctg agcgacttga cttccatcaa tatcatcttt tggagttgga attccgaaaa
     7981 cagcggttaa caatttcaag ggtgtgtaat ttttgtaatc gccgaatttc caaagctcca
     8041 gggtatcgag aaaattgttt tcccacggct ttctccccga tatttgaaat acgggaggaa
     8101 gttccaatcc attgataagc aggcgtctgc aaatataggg tatgtcaaat tccttgatgt
     8161 tgtgtccgca aagcatattg tcttttgatg agcaaaattt ggaaatcagg tcggaaaaat
     8221 tttttaaaag ttccttttca tcgtcggaag caaacgattt ggttctaaaa tacatggttt
     8281 ctccctgaaa atagataaaa cctacggaaa tacagacaat tttgccaaat tcggaataga
     8341 ttccggctga agtatcgaaa gcttcgcttg ccgatgtgtc ctccttgtat ttttcgggca
     8401 tcctgattct aagtttttga aatttctctt cccaaagttg ggccatttca ttagaaagct
     8461 cagaaaaact tgaagtttga ggaacagttt caatatcaaa aaacattatt ttttgcaaat
     8521 cggcgtttgt catattgttt tgcggttttt tttcttattt caggttgttt tcgatatatt
     8581 tggtaagaat atcaatagca atatgattat ctccaccctg tggaataatg aggtcggcaa
     8641 aacgtttggt gggttcaata aaaagttcat gcatgggttt aacggtatgc tcgtaacgtt
     8701 cgataacctt gttcaccgta cgaccgcgtt cttcaatatc gcggttgata acccgaatca
     8761 aacggtcatc gggatcggca tccacaaaaa ccttcagatc cattagttca cgtaattccg
     8821 gattgcacag caccaaaatt ccttccacaa tgattacttc gcgtggataa attgtaacgg
     8881 tttcggctgc tcgcgtgcac gtgagataag aataaatggg ctgttcgatg ggttttcctt
     8941 ctttgaggtc ttgcaaatgt tttatcatca gttcaaattc aatggaatcg ggatgatcaa
     9001 aattaatctc caaccttttt tcgagaggaa gaaaactgct gtcgcgataa tacgaatctt
     9061 gtggtaaaac cactacttct ccggcaggta atctttctat aattttgcga acgacggtgg
     9121 tttttcccga acctgttcct ccggcaattc ctatggtcaa catattgata ttttgtttta
     9181 tttcagagca ctaaaataag aatttatacg ctttctttca tttaattttc aatgtttttt
     9241 taatatttct tattgggata aattctatat gcctaaaaac aaataattta agctatgaaa
     9301 aaatttttat tagcttttta ttcttaaaga atccattgtc ttttctactg acaaaattct
     9361 tgggtaaaag accaacattc tataattttt gaaaagctgc tttcaaaggg aaatgatcgg
     9421 aatagggaac tttatctttt ttgaaattca ataaaaccag agacgggtca tggaaaatat
     9481 aatcgattcg aaatcgatag tgcttttcgt tgaaagtcca tccaaatcct tttccctgct
     9541 ccaagaaaca gtctttgagt tttccgcgaa tttttgtata tacatacgaa gaagggacat
     9601 cattgaaatc gccacaaaca acaacaggat ggggagaaga agaaataacc tttttaactt
     9661 catcggcctg aattgctctg agtttgtagg cggctccaag cttttttgaa aaataaacag
     9721 ttatgttcgt tgccaaatcg gtgttgaaat tatccttcaa ctgatagggc atggctttgt
     9781 ccttttccgt caactgattg gattcaagat gattgttgat tacccttatg gtttttccgt
     9841 gaatgtttat atcactataa atggaagcat tggctgaaga attgtagggt atggtttgtc
     9901 gattgataat gggaaattta gagaatgtgg caattcccaa caagcttttt ttttcatgtg
     9961 ctttgaaata gatatgatga tacggatatt tgctaaaagc tttcatggca tcttcttcgg
    10021 taagatattt ttcgtttgtg ctgaccataa attcctgtaa gcatacaatg tcggcatcgg
    10081 ttgaaagtac atattgaata accggattta tttttttgtt tgcttcatcg tgtttttttt
    10141 cgagtcccat catgtgagtg ttgtaactaa taatggtaaa gtctggctga attgaagaaa
    10201 ccggttgttt ttttaaatga aaagaaaaat ttgtttttaa ttgagggaaa gaaacaagca
    10261 ttaagctcag ggaaatgaga aaataccatt tctttagcac aatccataaa aggaaaaatg
    10321 caaaattaag aaaaaaaata atgggaaaaa caagtgcaaa ataagcaaaa aaagtaaata
    10381 ccgcaggact tacaaaacct gcaaaaacgc tgaaaagcaa agcgcatgct acaataatat
    10441 ttgcgctaat taaaccccat tttgtaattt tctttaggat cattttaccg tctgtttcaa
    10501 taattgattt tggggaaaga aacgatttcc gattggatcg acaaaaaatt tatcttggtt
    10561 ttccctgttc aaaaagtctt tttttttctt ctgccgtcaa gctgtcgtaa cccgatgttt
    10621 taattttgtc gagtattctg tctatttctt gcattcgtcg ggacttttcc atgttgaact
    10681 cctcatcggt catttttcgg taagaagttg aatatttggg tttttttatt ttgagttttt
    10741 ttctcgaaaa aagattcaca atgttatcca gcactccatt tagccatttg gtaatatcct
    10801 ttcctttacg taaagatacc acaaaaaggt aacccattaa agctcctccc aaatgagcaa
    10861 tttcacctcc ggcattgttg gacgtgattc cgaaaaaact tgtaaggatc gccaccaatg
    10921 caatgtattt caattttatg ccgccgagaa acagtatccg catttccaga ttcggtgaag
    10981 cgacggctga agctacaata atggccatta tagaaccgga agcacccaaa agtaaacctt
    11041 gtgaaacagc tgcatcgaaa taggggaaaa tattaaatga cagaaaataa aacaatccgg
    11101 ctacccatcc tcccaccaga tataaaccgg taagttgttt ttcggaataa tacatcaaaa
    11161 atatttttcc aaaccagtaa aggcacaaca tgttgaaaaa cagatgaagg aaaccttcgt
    11221 gcatgaacat gtaagtaacc ggtgtccaga aatgataaag caattgcctt ccatctgccg
    11281 gcatagccaa aaatggaaga agaaaaatac cactggcatt gaatagtttg aaagtgatta
    11341 aaacagcatg gtacagcaaa aacacaccga ggttgatata tatgagttta atcagatgac
    11401 ttccctgacg ataggataat ttaatattgt caaaaatatt catagcaaaa aatttgaaaa
    11461 taaaacaatg taaatcaata aattatattc tttatataaa ttttttaaga atacttacat
    11521 caatgtacac cttttttctt ccaatacaaa atcaaaaaaa ttccgaagag cataccgccc
    11581 aaatgggcaa aatgagcaac attatcaccc gtaaaaccag ccacccctaa aaataattcg
    11641 gctactgcat acatcaatac aaatattcgg gctctaatag gaatcgggag aaatataagc
    11701 ataagtttag catcggggaa aagccatcca aatgccagta ataaaccaaa aattgcacct
    11761 gaagcaccta tggttacggg tagattcaca aaaagatgct tcagttcaat taatgaagtg
    11821 gtgtctaaag ctgccaaatt gttgattcta aaaaaacggg acaatactcc ttcgtgagaa
    11881 aacaaggcat ctttgttccc ggtgtttatg gcttcgttga gtgctgaaat taccggctga
    11941 tattctacag accaaaaaag ttgctgtata agagcagctc caatacctgt aaccatgtaa
    12001 tacagcataa agcgtttggg accccaaaat tgttccaata ccgttccaaa cataaacaag
    12061 gcaaacatat tgaaaaaaat atgctcaaaa tttccgtgca aaaacatgta agtgatcagt
    12121 tgagcaggat taaactgttt cgatccccag taatgcatac cgagaatgtt gaccagatca
    12181 acatttatcc cccaacgcag aaaaaaacga ggtaaaacca aagttgccaa ccacaatatg
    12241 atgtttatta aaatcagatt cttaaccacc acaggtatag aaccaaaaaa agaggatctg
    12301 taactcatgc aatacttatt tttttattca agaaaattgt tcagacaaaa tcttctttcc
    12361 acaaccattt accaactttc aaaacattta atggagtata tagttcatta aatcgttttt
    12421 ttaaattgca aatgtagcga aaaaattaac taaaacatgt tttgtaacat attttgtaag
    12481 aaaaagtaaa aaatacagaa atttttttat tcaaatgctt gctatttgtt ttcaataatg
    12541 tacatttgta gctttaaaaa ggatttttta gtgtgtttaa ctttaaaaaa attttatgtt
    12601 atgaacaaag cagaattaat cagtgcggta gcagaaaaat ctgggctaag caaagctgat
    12661 gcaaagaaag caattgacgc tgttgttaaa agtatctctg aagctttaat tgcaggagat
    12721 aaagttagtt tagtaggttt tggcactttc tcggttgctg aacgtgcttc tcgttccggt
    12781 attaatccgg caaccaaaaa acccatcact atccctgcta aaaaggttgc aaaattcaaa
    12841 gccggtgcag aattaagcga tgctatcaat taatttgttt gttaaagttt taataaagga
    12901 ggaattattt ttttaattcc tcctttgttt ttgtaataac tcttttgctt ttttgttttt
    12961 agcttttctt taaaaagcaa aaatagacaa acctacctgc tcggctgcag attttccttt
    13021 cagattttca accagtttca gggcaaattc tatggcggcg ccggcagctt taccggtaat
    13081 aatattccct gactgaacaa cttttttatc ggatacaata gcacccttta gtgtttcttc
    13141 aaatccggga aaacaaacgg cttcctttcc cttcagcaat cccaattcgc ccaatatgga
    13201 aggtgcagcg caaatagccg ccaacttttt gccttcattg gcatgtttca gcaaaagttg
    13261 ttttaaaccg tcatgagcat ttaaattttt tgtccccggc attccgcccg gcagaaacag
    13321 caaatcggca tcagaaaaac tgacttcttc aaacaaacga tctgcctcaa ccaaaacacc
    13381 atgtgcacct ctcacttctt tttcgcgtga aacggaaatg gttgtaactt ctattcctgc
    13441 tcgacgcata atgtcaattg gagcgatggc ttcgatttct tcaaaaccat cagctaaaaa
    13501 taccattgct ttcataagtt tgtcattttt attatgttaa ggtcgttttt tatttttcca
    13561 catatttctg taaaacagcc acattccggt agtttatttt aatctgaaat gataggtgat
    13621 ggttccggta gctttcccct ttccaggact gaaacgggta ttttttgctg cttccatggc
    13681 tgcttttaaa attgttgcat cggaaatatt ggtgggaaca ccaatagaaa ctccctcaac
    13741 aactccgttt tcgtcaaccc gaatgttgac agtaacctca ccttccacat tttgttcata
    13801 tctgggggta atcagatttc ccaccaaatt tcgtccacta agcgaccagc tgttcccatt
    13861 tgaagttcct cttccggcag gatttccttt taataaattc tcatcgccca cattttcatt
    13921 ccctgatatc tttttatcaa atatcccttc cataatattg gcttttgata ttgcctcttg
    13981 ttcaagtctt tttttctcgg acaattctct ttcctgctct ctgttgtcag tctttttgtc
    14041 gagcgaaaca gaaggatctt tttgagtcaa aattttttca cttgcttttt tcgatactgc
    14101 atactcctct gcagcagaag gggaagctgc cctgctgcct ccttccaaag tttcaccaaa
    14161 actgaccata atgccttcag gttcaacaga tgttgaagcg tgcattttaa tgatcagcaa
    14221 tagcaccagc agcatcatac aaaagatgat tgacccaaca ataccatata attcggattt
    14281 tttcacacct tctcaatttt caaacggttt ggtggctata acaattttaa aaccatgttg
    14341 attggcgata tccaaaaccc ttactatttc cttgtaagca atattttgat cggcataaag
    14401 tgcaacaaaa gaaatactgt cttgtgaggc catatttaaa agatacgatt ccaacaattt
    14461 aggttcaata ggaacgggtt tttgatttcc tttggctacc gataactttc cttcagcatc
    14521 aatagcaact ttagcaatta tttgcttggt tgaagactta gatctacttt caggcaaatt
    14581 taattttatg tcgttaggag acgataaagt tgatgcaata acaaaaaaaa gcaataacag
    14641 gaaaataatg tctgtcatgg aggacatggc gaaacctgct tcaactttat ttcttttttt
    14701 taatgccatg ctgcgaatgt tttaaaatat ttattttaca ggttcattga gcaaatccaa
    14761 aaattccata gttttcgatt ccaggatgtt tactacagaa tctactctcg atacaaggta
    14821 attataggca aaatatgcca aaattccaac aatcaaaccg ccaatggtgg taaccatggc
    14881 ctgatacata ccaacggaaa gatcaccaag taagatgttt ccacctgctt tttgagaaag
    14941 attgaaaaaa gttgatacca ttccggtaac ggtgcccaaa aaacccagca tgggagcacc
    15001 acccgaaata gaagccataa taactaatcc cttttcgagt tttgcaattt ccatattacc
    15061 aacattttct atggctacca gaacatcatt caaaggatgc cccaatcggg tgattccttt
    15121 ttcaatcatt cttgcactgg gagaatttgt gtccctgcag agttttaatg cagtttcaat
    15181 ttttccttca taaatatagt ccttgattct ttccataaaa aaagcatctt ctttggaagc
    15241 ttgattgatc gtgatcagtc tttctataaa taaatatacg gcaaaagcca gcattaaagc
    15301 caaaattatc ataatgggac caccaaattt cgccattgtc cataggttta tttccgactg
    15361 tagcgggtcg gcaactgtag tggcaacatt tgctgaagct tgaagaataa gcagattaat
    15421 cataattatt tcaattttta tttttttgtt tttgtttcaa aattcatgcc ttactatttc
    15481 aactgatttt tgtactgttt tattgtttcc tgaagtccca aatacaaagc atcacaaatc
    15541 agggcatgac caatggaaac ttcatcgagc catggaatac aagcatgcaa ataatgcaga
    15601 ttttgaagat tcaaatcgtg tccggcattc agtcccaatc ccagtttttt ggctgctttg
    15661 gctgcttcaa taaatgtagc aactgccttt tctctgtttt ctgcgtattc gcgggcataa
    15721 ggttcggtat aaagttctac cctgtcggca cctgccaatg cggcatattc aacactttta
    15781 atatttgcat caacgaaaat ggaaacacga attccggcat cctgcaattc ggcagtaatg
    15841 tctttcaaaa aatcgagatg tttttccgta tcccatccgg cattggaagt aatggcttcg
    15901 ggaggatcgg gaaccaatgt aacctgtgta ggttttacca ttgtaaccag tttcataaag
    15961 ttgggagaag gataaccttc aatgttgaat tcggtctgca aaattggttt gagtgcccaa
    16021 acatcggaat agcggatatg tctttcgtcg ggtcgaggat gaacggttat gccatcggct
    16081 ccaaattttt cacaatcgat ggcggccttg cagacgtcgg gagtattgcc acctcgtgca
    16141 ttgcgcaagg tagctatttt atttatattt acacttaatt ttgtcatgtc aattgaattt
    16201 taattattca tttcttgatg gttaaataaa attaaacaaa caatagtatc tccgttccac
    16261 aatatgaaaa aaagagaaaa aatcattttg tttagaaaaa tatttatttg taaatgtcgt
    16321 tttcatttag ttcctttgtg taaagtttca gattcatttt ctctacaaaa ataactcaaa
    16381 attttgaagt cgcacttgaa gaattgtcta tgtttttgtt ttttaataaa aaactatgcg
    16441 ttttattaat tcaattcctt gttttacaga atgtttcttc tttttttgta aaaaaaattc
    16501 ttgatatgaa acccacatgt attacatata caaacacgaa tgaatataat attcagacat
    16561 gtgggttcca tcagaccatt aaaaaataaa ttatattctg atgaaagttg caatttttgg
    16621 aaatatatat aggccggtca tcattaacag tattaaagtg ctgttcgatt ttttttcgga
    16681 aaaacaaatt cctttaatgc ttgacagtga tttgtacgat ttccttgtac atataggcgg
    16741 ttatcggttg gaaaacgtgg aaaaaatcga gaatgacaat ttcaaagccg atgttgcttt
    16801 gagtattgga ggtgatggaa cttttttaaa tacggcggca agaatcggtg ataaagaaat
    16861 tcccattatc ggcatcaata ccggacggtt gggttttctt accgatgttt cgaatgatga
    16921 aattattgaa gcgttggaag ctcttttaga aggaaaattc agaatagaag aacgtacttt
    16981 acttttggtg aaaacttcgg acggaacgcc gttgcaatat ccttatgctt tgaacgaggt
    17041 gtcggttttg aaacaggatt cttcttccat gatcagcatc aatgccagcg tcaacggcga
    17101 attgattcat acctatcagg ctgatggttt attggtttca acgccaacag gctcaactgc
    17161 ttattctatg agtgttggag gcccaattgt tgtacctcag gcacaaaatt ttattctttc
    17221 gcctgttgcc tctcatagtt tgaatgtgag gcctttgata attcccgaca actggaacat
    17281 cgatcttgag gttcacagtc gtacacacag ctatcttgtc gcgctcgacg ggcgatcgaa
    17341 agtgttggaa caaactacac aattgcacat tgccaaagct ccttttacca taaaagttat
    17401 aaaacagctt cagcatactt ttttcgatac gttgaagaaa aagttaatgt ggggtgtcga
    17461 caagaggaat tagcaaatac gaaaaaaaca ggattatttc tctttttact ttgtttctga
    17521 aaaactttcc tttttttaag gtttagttac caacttcgga gagaaacttg attcttacaa
    17581 gccgtatttc cgtttcacta aaatcgtcta ttccaagctt atccaatgca tcgtctatgc
    17641 tgtcggtttc tgcagaatta aagtattcga aaatttcatc tacgcggtcc gcgtccataa
    17701 catcttccaa aaaataatcg atattaattt ttgtacctga atatacaatt gcctcaattt
    17761 catccagcag ttcagtaata tccaatcctt tggacaaggc aatatcgtcg agggcaattt
    17821 ttctgtcaat agcctgaata atagagactt taagtttcga tttattggct acagtacgta
    17881 ctctcaaatc ttcaggacga attatgtcgt tttccttcac atattctttg atgactttta
    17941 aaaattcatc tccgtatcgc ttggcttttc ctgccccaac ccccggtatg ttttgtagtt
    18001 catccattgt tatagggtat gtagtggcca tagcttccag cgaaggatcc tgaaaaatga
    18061 caaaaggcgg tacatccaat ttttttgaaa gttttttgcg taaatccttt aaaatggaaa
    18121 acaatacttc atcggcagcg ctggtgctgg caatattttt cagattcact tcctcatctt
    18181 cctcaaagtc attatcttta acaattggga acgaagtagg atttttcaaa taatcttttc
    18241 ctttgggggt gattttgagc actccataag tttccacatc ctttgttata tatccgctga
    18301 taagggcttg tcgaataaca gcctgaagca atttttcatc ttcatcggag gcggctccga
    18361 agttttccaa ttcattgtgt tgataggaaa ctacgtcaga tgtttcattt cccttaagaa
    18421 tgtccacaat gtgttcggct ttgaattttt cttttaaaat agaaatggtt tcaagaacca
    18481 aagtcaacaa ttcttgcgct tccataaatt tttggggttt catacaatta tcacaattac
    18541 cacaattatc ttctttgtat tcttcaccga aatagtgaag taaaagtttt cgtcggcaga
    18601 tggaagattc ggcataagcc tgtgtttcta ttaggagttg attcccaatt tcctgttcgg
    18661 caagtggttt tccctgcata aattttcgca gtttttccag atccttataa gaataaaaag
    18721 ctatgcactg accttcgcct ccatcacgtc cgctgcgtcc ggtttcctga taatatcctt
    18781 ccagactttt gggcatgtcg tagtgtatca catatctgac gtcgggttta tcaattccca
    18841 tgccaaaagc gatagttgcc actatgacgc ttgcttcttc cattaaaaac ttgtcctggt
    18901 tttcgctccg ctgagcagca tccatgccgg catgataggg taaagccgaa attccgttta
    18961 catgcaacat ttcggccagt tcttccactt tttttctgct caggcaatag attatgcctg
    19021 attttccttc ttgagaacgt acaaatttaa taatatcctt gtcgatgttg ttcgtttttt
    19081 gacgaacttc ataatacaga ttgggacggt tgaaagaaga tttgaaaacc ttagcatcca
    19141 acattcccag attcttttga atatcgtgtt gcacttttgg agtggcagtg gcagtaagag
    19201 ctatgatggg agcaattcct atttcattga caataggacg aatacgtcga tattcgggac
    19261 gaaaatcgtg tccccattcc gaaatacagt gtgcttcatc aatagcgtaa aaggaaattt
    19321 ttatatgttt gagaaattcg atattttctt cctttgtgag tgattcggga gcaacatata
    19381 gcaatttagt ttttccgctc aggatgtcac attttacttg ctcaatagct tgtttcgaca
    19441 acgaagaatt gataaagtga gccacgccat cttcttcact gaaattgcgc atggcatcca
    19501 cttgattttt catcagagca atcagaggag aaatgacaat ggccgttcca tccattaaaa
    19561 gagaaggaag ctgataacac aatgatttac ctcctcctgt aggcataagg acgaaagtgt
    19621 cgtttccatt gagcacattt tgaataattt gttcctgatt ccccttgaag gtatcaaaac
    19681 caaaatattt ttttagctcc ttagctaatg agactttttt taacatgaaa aaaatagttt
    19741 gtcaaaaaaa acaagcacga aattagtaaa tgttgatgaa ttatcagaac tgtttccctg
    19801 aatttttttg aattattttc ttggatttgt agaaaaggaa ttaatagctt aatcatgaag
    19861 caaaattata aactcttttt aaacctacaa gtttttttca aaaaaaatac aggatatata
    19921 caacttaaat tcaattagta aatgcaactt tgacgggagg atagggctca cccgtaaaaa
    19981 tatgtgttta agcatagata ttagtcaatc tgaagagaaa gaatttaata tcgataactc
    20041 ctctcaaaga agctctgaag gatttaattt tagcattgaa actttcggca gaggcattgg
    20101 tacttctatt gataaagaaa ttaagaactt cttcgtgatg ttcataaact gtagcagcta
    20161 ttgtattaaa agacttgaat tggctttcag ccacatttga gagatacttt atcttgttgt
    20221 tgtctttaag aaaaattgaa ttgagaactc ccgatttttc ttcaattcca acaatatcaa
    20281 aatcgtctaa tatctctttc ggaagaatgt aacgggcaag tgcctcaagc tgttcgtaag
    20341 tggacatata aaaagtattt tttcacaaag atacttttct ttttctacaa atttatacag
    20401 gtgagccatt tttgtttgcc cgaaaatatg aaaaatgtta cttttgcaca acgtaaaaac
    20461 aactctacaa catgaatata ttacttctgg gttcaggagg tcgtgaacat gctttggcat
    20521 ggaaaattgc tcaaagttca cagcttacaa atctttatat tgctcccgga aatcccggca
    20581 cgtcaacgct gggaaccaat ctcccctttt cggcagatga ttttcaatcc atcaaaatag
    20641 cagtgttgga acatgaaatc gattgggtaa ttgttgggtc ggaagatcct ttggtgaaag
    20701 ggattgccga tttttttgcc tctgacaaac aattaaaaaa cgtatctgtg atcgggccgt
    20761 caaaaattgc tgcgcaactg gaaggcagta aaaatttcgc caaaaagttt atgctcagac
    20821 atggcatccc gacagccaaa tatttcagcg ttacctcaga aactattgac gaaggcatac
    20881 aatttctcca gcaactttcc tctccttacg tactgaaagc cgacggactg gctgcaggga
    20941 aaggcgtgct gattatcgat agcttggaag aagctgaaca ggaattaaaa tccatgctgg
    21001 aaggaaaatt tggaacagca agtaaaaccg ttgtaattga gcaatttctt tccggaattg
    21061 aatgttcggt atttgtgata acggatggaa ataattacaa aattctgccc gaagccaaag
    21121 attacaagcg aataggtgaa ggtgataccg gattaaatac cggaggaatg ggcgctgttt
    21181 cgccggtttc gttcgccgat aaatatttca tgaagaaagt agaacaaaat attattcgac
    21241 ccactatcaa cggattgaga gaagaaggaa ttgtatataa agggtttatt ttctttggtt
    21301 tgataaatgt taaaggtcaa ccttacgtta ttgaatataa tgttcggatg ggcgatcctg
    21361 aaacagaagc cgtcatgtta cgaataaaat ccgatttcgt tgatttactg ggaggtgtgg
    21421 taaatgaaaa tttggacgaa aaagaaatta aaattgacga gcgtttcgca gccacagtaa
    21481 tgctggtttc cggcggctat cctcaagctt atgaaaaggg caaggaaatt tccggtttgg
    21541 ataaagtgga aggaagtatt atttttcatg ccggcactgc cttgaaaaac gggaaactgg
    21601 taaccaatgg cggcagagta atggccatta gttcttatgg aaacaccatg aaagaagcgc
    21661 tggaaatatc attgagaaat gccgaaaaaa ttaattttga aggaaaatat ttcagaaaag
    21721 atatcggatt cgatttataa aagcttggaa tttgttgctg caaataaaaa ttaatcccaa
    21781 tgaaaaaaat tgttgtcgct attgatgggt tttcttccgg cggtaaaagt actatggcaa
    21841 aagatcttgc acgagatgta ggatatactt acattgattc gggagccatg tatcgtgcag
    21901 ttgccttgta tgccatgcgc aatggttgga taacagaaaa tgatattgat atcgaagcac
    21961 tgaaaaaaaa tatcggctct ttaaaaatcg attttaaaac caatgagcat ggagtttccg
    22021 aaacttatct caatggcgaa aatgtcgaaa aagaaatcag aacattggaa gttgccaatg
    22081 cagccagccg agtaagtgct ttggactttg taaggagaga actggtgcgg cagcagcaac
    22141 aaatgggttt gcaaaaagga attgtaatgg atggaagaga tattggaact gtagtttttc
    22201 ctgaagccga gctgaagatt tttttaaccg cctctcccga aatccgagct caacgaagaa
    22261 tggaagaaat gctggccaga ggggaaaaag tagattttca aaaagtgctt gccaatataa
    22321 aagagcggga cgaaagagat cttcatcgtt cagaaagccc actcaaaaag gcccccgatg
    22381 ccattgttat cgacaattcc aatctgactc gtaacgaaca gcgagagttg ctcaaaactt
    22441 tattttatca aaaaacgtta gaataataag ctgattcaat taaagtttca gtgttattca
    22501 tctgtttttt taatttttat aaagcttcaa aattttgaac atcgaaatag acaacaaatc
    22561 cggattttgc tttggcgtgg ttaaagccat tgaaaaagca gaagaagaat tggaaaaagg
    22621 ctcaactcta tactgtttgg gagatattgt gcacaacgaa gaagaagtgg aacgacttca
    22681 gcgattggga ctcgttactg tcaatcatga gcaatacaac caactgcata atgtgaaagt
    22741 gctgattcgc gctcacggag agcctccaag cacatacgaa acagccagaa agaataatat
    22801 taccctgatt gatgctacct gtccggtggt actgaaattg caatcgcgca tcaagaaaaa
    22861 ctatgaagaa ttggcttcgg aagacggaca aatagttatt tacggacaaa caggacacgc
    22921 tgaagtcaaa ggactggtag ggcaaaccga tggtaaagcc attgtgatag aggaagaaaa
    22981 agatttggat aaaatcgatt tctcaaagaa agtacatttg ttttctcaga caaccaaaga
    23041 tattgaacat tatcatcatc tgatagagga aatttcaaaa cgcatggtga aaaaggatga
    23101 ttttaaattt agcgacacca tttgccgtca agtagcaaac agaataccaa atattattaa
    23161 ttttgcacaa cagaacgacg ttattctttt tgtaggtggc aagcaaagct caaacggaaa
    23221 agttctttat gaacattgca aaagcattaa tccacagaca tattatattt ccgattgtaa
    23281 acaagtggat gaactcgaga ttcaaaagga taaaaaaatt ggaatttgtg gggctacttc
    23341 cacaccaaaa tggttgatgg attgcattgc caaacgtttg gaagaaaggt tagcctgatt
    23401 tttattagtt ttttgataat caattaatca acaaaataga aatgaaatac gaaattaaat
    23461 gtatcggatt acttacttcc ggaggtgatg ctcccggaat gaatgctgct atcagggcag
    23521 taacacgtac ggcaattttc aacggtattc gtgtaaaagc gatttaccga ggttacaagg
    23581 gactgataac cggagaaatc aaagaattca agaccgaaaa cgtgagcaac attattcagc
    23641 aaggaggcac gattattaaa acggccaggt gcgctgaatt tatgacaccc gaaggaagaa
    23701 aaatggctta cgaaactatg aggaaagaag aaattgatgc gttgattgtt atcggaggtg
    23761 atggaacttt aaccggtgct cgaatttttg cacaagaatt taacgtaccc attgtaggct
    23821 tgccggggac aatagacaac gatttatggg gaacggatgt taccattggt tatgatacag
    23881 ccttgaatac cattatcgat gcagtagaca aaattcggga tacggcttct tcccacgaac
    23941 gtttattctt tatagaagtc atgggacgcg atgcaggttt tctggcttta agcagtgccc
    24001 tggcttcagg tgcggaggct gccattattc cagaaaggga aaccaaagtc gatcagttgg
    24061 aagaactgat taaaaacggc ttcagaaaat cgaaaaacag cagcattgtc attgttgccg
    24121 aaagtgaaat taccggcgga gccaatggct tggctgaaag aatgcgccgc gaacatcccg
    24181 aatatgatgt acgcgtaaca attttgggac atattcagcg gggtggttca cctactgctt
    24241 cggacagaat tttggccagc cgcatgggtg tggcagccgt agatgctttg ctggatgagc
    24301 agcgcagtat tatgattggc atcgtgaacg atgaaattgt tcatgtaccg tttacaaaag
    24361 ccattaaatt cgacaagccg gtaaatgaag atttattggg cgtcatccaa atcctgtcaa
    24421 tttaacaatt ttcattcgct aaatttattg atgaatgttt ttttgttttt tcttttacca
    24481 aacattgtag ttccctgaaa aattaaaaac cactgcctgc gatattttat ctagtcagtg
    24541 gttttttatt taacattttt ttaaactcag taacccaaaa ttttcaacat tgacttgtag
    24601 ctttgttctt tggcaaaaag tttggtaaaa tactctccgt cttcatcctt gtcgataatt
    24661 accatttttg gagccggtat caaacaatgc tgtatgccgc catatccgct caacgattct
    24721 tgataagcgc cagtatggaa aaatccaata tattgttccc gcccttcttc cattttcgga
    24781 aggaaaatag catttgaatg aacttcggca ttgtaaaagt cttcactgtc gcaggtcagg
    24841 ccgccaagat tgacgcgttc gtattctgaa tcccaattgt tgatagccaa aaatacatac
    24901 cgctgattga ttgcccatgt atctggcagt gtagtcataa acgaactgtc gatcatgtcc
    24961 cacaattcgc ggtcgttttg ttttttctga ttgacaatgg aataaagcat ggctccactt
    25021 tcgccaacgg tgtaggaacc aaattcggta aatatatttg gttcgggaac tccgttctga
    25081 gtgcagatgt tttttatctg tgctatgatt tcttcggcca tgtattcgta atcgtacgac
    25141 atatccaagt gggtctgaat cgggaaacct ccaccgatat ttaaactgtc gagttcagga
    25201 cattttttct tgagttcaca gtacaaattt acggctttac tcagttcatt ccagtagtat
    25261 gccgtatccc tgactcccgt attgatgaaa aaatgcaaaa ttttcagttc gacctgcggg
    25321 ttgttggcta tgtgctttgt gtagtaggga ataatgtcgt tgtatcgaat accaagtctg
    25381 gaagtataaa aatcaaattt cggttcttct tccgcagcaa ttctgattcc tatttttaat
    25441 ttcttatcga aatctttcag cagcaaatcc aattcaaatt tattgtccaa tatcggtact
    25501 acattagaaa atcccaaacg tatcagattt tgaatatttt ctatatattg tggacgtttg
    25561 aacccgttgc agataatata tgtttccggc ttcagtaatc cttgttcata aagcgattcc
    25621 atgatgttga tatcgaaagc ggaagaagtt tccagatgta cgtcatgttt caaaacttct
    25681 tccattacaa aagagaaatg agaactttta gtacaataac agtacatata atctccctga
    25741 taatcaactt ttgccatagc cacattaaac atacgtcggg ctttttgtat attctcggct
    25801 attttcggca ggtaggctat tctcaacgga gttccatact gcttgatgac atccatcagt
    25861 ggaacaccac accaccagag ttcattgtca atgacattga attcatcatt ggggaattca
    25921 aaagtctgtt caatcagatc aatatacttg tttttcatcg cttgttttct tgattttcta
    25981 taaatataac ttacttaaat tgcaataaaa tgtattatcc gattcagtta aaattgatgt
    26041 tttagctaaa tgtgtgcaaa attaatactt attttttata tggcaatatt aggtgaacaa
    26101 aaaatcattt cttgatcaac actttaatat ataatactaa tatgtttata atcaattaat
    26161 taatataaaa ttttttgaaa agtttaactt tatacttata ggttaacttg tttattattt
    26221 tatcatttgc caagttgaaa atataaaatt cagaaaatcc aagttattta cattgctctt
    26281 tttttattta tccaattaat tttgtttgat tgtttatcgt ttttttgaat tgggactaag
    26341 gttaaattct tattttatat atcgagatag aattctttaa atcaattcat tttacgaatt
    26401 ccaaaattaa tgatctattt taaataaaat gaaagagggg aaatactttt atctaatcag
    26461 atttggttgg agtttagagt ggatctacat ctatttgtaa atgaaccgat ttgaatgaag
    26521 gttgcgagag ggaaagattg atgcaatctc gtatcaattt ttttgttttt tcaggagatg
    26581 cttctatttc tatttttaat agaatatgtt taatataaaa gttttgaatt ttaggaatta
    26641 gcggatcact gggaccctgt accctgcggc caaaaacgct ccgaagcgag ttggtcagtt
    26701 gatgagaagc ttcctgaagt atctgtctgt cgtgatgacg aactataagt aaaatcagtc
    26761 gtgtataggg tggatatttg aactgtttgc gctcttcgca ctggtttgca aacattcctt
    26821 cgtagtcgtt ggtcattact tgttgaataa ttgggtgttt cggttgatag gtttgcagaa
    26881 tcacctttcc ctgtttatcc tttctgccgg cacgaccgct aacttgtgat agcaattgaa
    26941 aagctcgttc gtgtgatcga aaatcgggaa aattcagaat attatctgcg tttagaattc
    27001 ctaccaaact aactttgtcg aaatccagcc ctttggtaat catttgagtg ccaatcaaaa
    27061 tatcgatttt atactcttca aaatcggtga taatcgtttc atacgatttc cttgaacggg
    27121 tggtatccag atccattcgg ctaattcgga cttcgggaaa gatttttttt atttcatctt
    27181 caattttttc tgttccgaat ccgtgcgtgg agaaagccgc cggcgttccg cagaccggac
    27241 aggaagcagg ataagattca gtgtatccgc aataatggca ggtcagagca ttggtagttt
    27301 tatgataagt tagactgaca tcgcaatttt tgcatttggg tatgtagttg cagcttttac
    27361 attccagata gggcgcatac ccccttcgat tttgaaaaag aattatttgt tctttgtttt
    27421 gaagagtttt ctgcatttca tgcatcagca aatcggaaaa atggccgttc atttgttttt
    27481 tgtgataagc ttctttcaaa tctacaacca cgatttcggg caacgaaatt tcttcaaatc
    27541 gccgatcaag ttttacgagt ccgtatttgc ccgtaacagc gttgaaatag gattctattg
    27601 ccggtgttgc cgaaccgagc aaagtgtgtg ctccgtgcat ggaagccagc acaatggcag
    27661 cgttacgtgc gtgatagcga ggagccgggt caaattgttt gtaacttgtt tcgtattctt
    27721 cgtcaacgat taccagacct aattgtcgga aaggcaaaaa aatggaggaa cgaacgccta
    27781 taataacttc ataacctttg tcgttcagca aattgttcca aatttcggtg cgttctgcgt
    27841 cggaaaattt tgaatggtaa actcccagct ttttaccgaa aattcttttc agtcgggcag
    27901 tgagttgggt ggttagtgca atttcgggca ccaggtaaag tgcctgtttc ccttcgttta
    27961 aaactttttg aatcaaatga atataaagtt ccgttttccc gctcgaagta acgccatgca
    28021 gcaataccac cggtttttct ttaaattttt tttcgatttc ttgcagtgct tttgtttgaa
    28081 actcgttaag aatagagggt ttctgaagtt cgttttcaga aaagtcgaga cgtccgacaa
    28141 ttttctcgta ggtttcaaaa atctttttat cggtgagtgc tttcagaaca gcgggagaaa
    28201 tgcctgattt ttcaagaagt gttttgcggg aaacttcccg gacatttttt ggatcaagac
    28261 actttgaaag atcaatatag ttcatcagca aacttaactg tttttttgcc gatttcagtt
    28321 ggtcaaaaat ttcgtgaagt ttttcatcct gttgaatttc aggacgaaga cgtatgtatg
    28381 tttcagtttt gggatgaaat ttttcggtaa gctgttcgct gatttcgatg acgccttttt
    28441 ccagcagtaa ttttagttga ggtaaaacgt ttgaaattcc gctatctttt gccaacgctg
    28501 acaatgaaat gggctttttc tgatttaaaa ggacatccag aatttttttt tcgcgttctg
    28561 aaaaatggac gttggtatca aaatccggct tcacccgtac aagagtttca ctttccagtt
    28621 taagaccggc cggtaaagcc gattgataaa cttcccccaa atgtgcctga taataattgg
    28681 ctatccattc ccaaaatttg atttgaggtc ggcgcaaaat gggttcctca tccaaaatac
    28741 cgacaatttc ttttatttga taagtcgttt ccggacgaag aatatgaaca taatatacaa
    28801 ttccggaata taattttttt ttaccaaatg gtactacaac tcgcatacct tgttgtatcg
    28861 agttctcatc tgcttcggga acagcatagg taaaagtatt gggcaatggc aaaggcaata
    28921 tgacttcaat gtatttcaaa agaaccgatt tctgttttta taattgaaca aaatataagt
    28981 tggaaaaaac gtttaaaaag tttagatgga tattgtttca gcaaagaata atcagcagca
    29041 tttaattgta aatgaatttg attaaaagtg gttcggtgtt tttcaatgct gcatgaagga
    29101 tataaattcc tttttgcatg tttttcatag aaatgcggtt ggttgacgcg aaactttgta
    29161 tggcagtgcc ggcgctgtca aataaagtaa ctttactgaa tgttttatcg aaaagtatga
    29221 ctttttgcaa gtcatcataa cgccatccgg tttttagttg gagcggatgg gtcgattgac
    29281 tgacttcttt aaaagcatcg agggcaatag taggttttcc tgtattgtcg aaagcgccca
    29341 atgtatatcc gttccatccg ggataacact gcggctccca atataatacg cccaatcctt
    29401 ttccttgcga aacatttttg gttttgttaa tcaaatcgga aagaaaatct ctgcatgctt
    29461 ccggagcatc ccattgcata ccgcattcca ctatcattac ttctgtgccg tatcgactta
    29521 ccatgtcgtt catattgttg aaacaactga tattgcaatt tctccagtcg tttttggtcg
    29581 tgtaccaaaa gggataaacc gacatgccga taacgtccca tttcccgcca taattcttta
    29641 acgtgtcgaa aaaccaacgg tacaaactgt tgctatagcc attgggtaag tggacgataa
    29701 ctttggcatt ggggaaaacg gattttacgg catcatatcc tgcattgttt aactcggcgt
    29761 agttttgtgg attttttgaa attcttccat cttcccaaag cattccatca gaagtttcat
    29821 tgccaacctg aacccattcc ggagttatat cattgttttt cagaaaagta agtacttcgt
    29881 tagtgtgttg ggaaagagct gttttcaggt cggcaaaaga aagattggac catgctgcag
    29941 gtttggtttg ttttccgggg tcggcccaag agtcgctata atgaaaatca atcataatgc
    30001 gcatgcccaa atcgtttgct cgttgtgctt tcaaaagtac atcctgagcg ttgcaccatc
    30061 catcggcagg attgacccat acacggagac ggatggtatt cattcccaaa cttttcagta
    30121 acgtcataca ttccgtttcc actccttcgg cagtataaaa tttttttccg gcagcttcca
    30181 tttgtgttac ccagcttaca tcagctcctt tggcaaagtc ctgcgaaaga gtttttgaga
    30241 aaaatccagt caatagtagg aataaacaga ttattttctc aaaattcggg aaataaaatt
    30301 tttttgtatg tgttttcatg tcgttttatc gaaataatgt tgaagactgt gaacattgca
    30361 aatgtagaaa attttgttgg taaaatccac aattcttatg aagaactaaa ttctttgtga
    30421 taaaaaaatc tttttttaat gcaaatcgat cgaatcgtta tgttgtactt ttcagagtaa
    30481 tcgtagaaaa tttatatcta tttttaacta cttttgtaaa tcataaaatt acgacagttg
    30541 ttgttcgtaa aatttgataa ccaaaatatt ttttacaatc catttactta aatatttatc
    30601 gttatgaagt caaagtattt tgttaatatt ttaattatga ctatgttgtg tttttacatg
    30661 cagcaattga atgcgcaaga ctggccaaat ttaggtcgtt tcagagcaga aaatgaaaaa
    30721 ttgggagccc ctgataaagg tgaaaaaaga gtagttttct tgggcaattc catcaccgaa
    30781 ggttggatta atattcgtcc cgattttttt aaaaatcgtt cctatatcaa tcggggtatc
    30841 agcggtcaga caacccctca aatgcttttg cgttttcgtc aggatgtgat taaactgcaa
    30901 ccggcggtag tagtaatttt agccggcacc aacgatattg caggcaatac agggccttca
    30961 acactcgaaa tgattgaaga caatctttct tctatgtcag aattggcacg ggttcacaat
    31021 attaaggtgg ttctttcctc cgtattgcct gcctatgatt atccttggcg tcggggtttg
    31081 cagccggctg agaaaattgt aactctgaac gcatggatta aagattattg tcagaaaaac
    31141 ggatttgttt atctggatta tttctcggca atggcagatg aaagaaacgg tttaaaatcc
    31201 gaattttccg aagatggagt tcatcccaat caaaaaggct atcaggtgat ggaaccactt
    31261 gctgaagccg ctattcaaaa agcattgaaa cagaacttca aaaaatgccc ctgtaataaa
    31321 taagtttctg taaaaaatta taaatcaaat cataaacttt ttgtatcttt gtgcccgtat
    31381 ttttttaagt caacataaag tctaatctac tatttattag taaaaaaaca aatttttaga
    31441 atggaagaaa accaaatcaa tttagaaaag aacgaatctg ccctgacaga aaatgtttct
    31501 ccagaagctg ttgaagccaa aaaagaagaa actcaagagg taaatgaagt tctgtctccc
    31561 gaagaagaaa gtgttgcaga atcgcatgca gcagaaccaa ttgttgcaga aacacccgaa
    31621 gtttctgtac ctgaagaacc tgtaaaggaa tcagttgccc cagttcaagc tgaaataacc
    31681 cctgaagaat ttgactggga tgcttatgaa aaaggaaata ccctgactac tgaagcaaaa
    31741 aaggaattgg aaaatgttta tgacaaaacc ctgaatgccg ttaaggacaa agaagtggta
    31801 gaaggaacgg tgatttccat caacaaacgt gaagttgttg taaatatcgg ttataaatcc
    31861 gatggcgttg ttcccatgag cgaatttcga tacaatcccg atctgaaagt cggcgataag
    31921 gttgaagttt atattgaaag tcaggaagac aagaaaggcc aattattgct ttcacacaag
    31981 caggcacgta ttagtcgggc atgggatcgc gtaaatgcag ctctcgaatc gcaggaagta
    32041 gttaaaggtt tcattaaatg ccgtaccaaa ggcggtatga ttgtcgatgt gtttggtatc
    32101 gaagcatttt tgcccggttc acaaatcgat gttaaaccta ttcgcgatta cgatgctttc
    32161 gtcaacaaaa ctatggaatt caaagttgta aaaatcaatc aggaattccg aaatgttgtc
    32221 gtatcgcaca aagctctgat agaagaagaa ttggagcaac agaaaaaaga aattatcagc
    32281 aaattgcaga aaggacaaat tctcgaaggt gttgtcaaga atatcacttc ctatggcgta
    32341 tttatcgact tgggaggcgt ggacggcttg attcacatta ccgatttgtc gtggggacgt
    32401 atcaatcatc caagtgaagt tgtttcgctg gacgaaaaaa tcaacgtcgt tattctggac
    32461 tttgatgaag agaaaaaacg tattgctttg ggtttgaaac agcttactcc acatccttgg
    32521 gatgcgttgg atcccaattt aaaagtaggc gacaaggtaa aaggcaaagt ggttgtgatt
    32581 accgattatg gtgcatttgt agaaattgct ccaggagtgg aaggattgat tcacgtatca
    32641 gaaatgagtt ggtcgcaaca tcttcgttcg gcacaagact ttttgaaagt tggcgacgaa
    32701 gtggaagcca ttattttgac gctcgaccgc gatgaacgta aaatgtcgtt gggcatcaag
    32761 cagctgaaat ccgatccttg ggaaaatata gaagaacgtt atccggttgg gagcaaacat
    32821 gttgccaaag ttcgcaattt taccaatttc ggcgtatttg tagagattga agaaggcgta
    32881 gatggcttga ttcatatttc cgatttgtcg tggacaaaga aaatcaaaca tccttctgaa
    32941 tttacgcaaa ttggaagtga aatcgaagta gtagtactcg atattgacaa ggaaaatcgt
    33001 cgtttgagtt tgggacacaa acagttggaa gaaaatccgt gggatgcgct cgaaagcgta
    33061 tttgctgtgg atagtattca cgacggcgta gttgttgaaa tgctcgataa aggtgctgtt
    33121 attgctttgc cttatggtgt agaaggattt gcttcagcca aacagttggt aaaggaagac
    33181 ggtacacagg cacaggttga cgaaaaactt cctttcaaag ttatcgaatt caacaaggat
    33241 gcaaagaaaa ttgtactttc ccacacacgt attcacgaag acctgaaacg tgctgccaaa
    33301 actgaaagtg cagaaggtga aacttcgact tctccaaaga aatcggttac caaacgtaca
    33361 aagaaagaag aatcttcaac cgtgcagact caggctatcg aaaaaactac ttttggagat
    33421 attcagtcat tggtagattt gaaagatcag atggaaagtg aagccgctaa aaaattggcc
    33481 aaaaaggaaa caaaatccaa aaaagctgag ccagctgaaa aagaagaacc tgcagaagta
    33541 aagatggcag aggaaacaaa agtggaagaa ccttctgaaa aacctgccga aacggaagaa
    33601 aagtcagaaa aacctcagaa atcgagtaag aaggaaaaaa ccgaaaaggt tgaagaaaag
    33661 gttgttgaag cagaagaaac acctgcttct gaagaagctg aaacttcaga gaaacctatc
    33721 gaataacatt cggtaattct gattcataat gtaaaaaaag ctgtttgatt ttttcaagca
    33781 gctttcttta gttttattgt ttaagaaaat tattgaatca agcggaaagt tttgcttcca
    33841 aattttgttt caatgctgtc agaaattctt cggtactcag ataatcggtt cggctaactt
    33901 tttctccatg aacaagtata gcaagatctt ttgtcatttt tcccgattct acagtttgta
    33961 cacagacttc ttcaagtttt tttgcgaaat cgattagtgc attattgcca tccagttttc
    34021 cgcgatgtgc cagtccgcgt gtccaagcaa aaatggaagc aataggattg gtagaagttt
    34081 cttttccttg ctgataaagt ctgtaatgac gtgtaactgt tccatgtgcg gcttctgcct
    34141 caatggtttt tccgtcgggc gtaatcagaa ccgacgacat cagtcccagt gaaccaaatc
    34201 cttgagcaat agaatcggat tgaacatcgc cgtcgtagtt tttgcaagcc catacaaagc
    34261 cgccattcca tttgagaaca gaagctacca tatcgtcaat cagacgatgt tcgtaagtga
    34321 tgccggcatt ttgaaaagct tccctgaatt ctttttcgta aacttcctga aaaatatcct
    34381 taaaacggcc atcataagtt ttaagaatgg tgttttttgt ggacaaatac aagggccatt
    34441 ttttatcgag tgctgcctga aaggacgagc gggcaaatcc ataaattgat tcatcggtat
    34501 tgtacatact catggctact ccatctcctt gaaaattata aacttcccac tcctgtgttt
    34561 ctccgttttc ggcagtaaaa gtcattttta gtgttccctt tcctttgatg agtacatcgg
    34621 ttgccttgta ctgatcgcca aaagcatgac ggccaatcac tatgggtttt gtccaattag
    34681 gcaccagtct gggaatgttg gaacaaatga taggttcgcg aaaaacagtt ccacctatta
    34741 tatttctgat tgttccgttg ggcgatttcc acattttttt caatccaaat tccttcactc
    34801 ttgcttcgtc aggtgttatg gtggcacatt taattcccac attgtatttt tttatggcgt
    34861 gggcagcctc aattgtaact tcatcgttgg taagatcgcg attttgtatg cttaaatcgt
    34921 aatattttat atcaatatcc agatagggaa ggatgagctg ctctttgata attcgccaga
    34981 taatgcgcgt catttcgtct ccatccagtt caactaccgg ttgttcaact tttatttttt
    35041 tcatactttt ccttttaaaa gccgaaagga tttttgttta aaaaatcctt tcaactattg
    35101 catttaacgt ttttcgagag gaataaattg ctgaatttcg ttgacattct tataagcagg
    35161 acgaataatt cttttactgg taaagttgag ttcttcgatg cgatgggcac accagccaac
    35221 aatgcgggcc attgcaaaaa taggcgtgta aacttctttc g
//
LOCUS       DAOTAX010000015        35025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1000_length_35025_cov_30.767772, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000015 DAOTAX010000000
VERSION     DAOTAX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 35025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35025
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_35025_cov_30.767772"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1347)
                     /locus_tag="PLM04_03030"
     CDS             complement(85..1347)
                     /locus_tag="PLM04_03030"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HON02084.1"
                     /translation="MQTMDSFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAIPTLK
                     KILNDGGCVIIASHMGRPKKNPDPKYSLKYILPHVSKLLGIEVKFAPDCIGPETAAMV
                     SALKPGEALMLENLRFYEEEEGKPRLPETATEEEKAAAKKELKERQKKFTATLASYAD
                     CYVNDAFGTAHRAHASTALIADYFDADHKMFGYLMEKEVAAVEKIMNNTEHPFTAIMG
                     GSKVSSKIDIIENLLNKVDNLIIGGGMTYTFVKALGGKIGSSICEEDKLDLALSLLEK
                     AKSKGVNMVLAVDAKIADAFSNDANTKFVPVNEIPDNWEGLDIGPETEKLFADVIKSS
                     KTILWNGPVGVFEFDNFTHGSLAVAQAIVEATKNGAFSLIGGGDSVACINKFGLADGV
                     SYVSTGGGALLEAIEGKVLPGIKAIRGY"
     gene            complement(1469..1888)
                     /locus_tag="PLM04_03035"
     CDS             complement(1469..1888)
                     /locus_tag="PLM04_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HON02085.1"
                     /translation="MATHITKTIWRKDNIFDTDVDGHIITIDLGKEAGGEDAGPRPKK
                     LLLVAAAGCSGLDVVSLLKKMRVELKGFDIQIEAQMSEEHPKQYTSMKVIYEFEGDNL
                     PMEKLEKAVTLSYEKYCGVMAMYKKAIPISWEIRIKS"
     gene            complement(2622..3107)
                     /locus_tag="PLM04_03040"
     CDS             complement(2622..3107)
                     /locus_tag="PLM04_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HON02086.1"
                     /translation="MKLSKFILAFLVLSALVFALPACKSKQKVVEIPGANAKAGTSTS
                     ENQQKTTSPVIVTQPTTSTENVPEITRSESFSLAEGETNQSAMNYKYHVVVGSFRNQS
                     NAKGLQRTLSNEGYNPVIVINEQGMFRVIMNSFNTYNEAHVFISKIQSRFPDAWVLIK
                     K"
     gene            3392..4126
                     /locus_tag="PLM04_03045"
     CDS             3392..4126
                     /locus_tag="PLM04_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HON02087.1"
                     /translation="MSGFAKWIGGGLGWALGGPIGALIGFAIGSFIGAKIETVEKSQK
                     RRTTTTSGDFKISLLVLIACMMKADGHVKKSELEIVKRFLVDNFGEEGALEALSILKN
                     LLEKDIDERQVAEQIYRYTNYSTRLELIHLLFDIAYADHQVSTSELTLIERIAGIFRI
                     SGLDFESLKAPYIKQVDINWAYKALEIEPSASVDEIKKAYRKMAMKYHPDKVNTLGED
                     VKKKATEKFRSINEAYETLKKERKFT"
     gene            4173..5639
                     /gene="cysS"
                     /locus_tag="PLM04_03050"
     CDS             4173..5639
                     /gene="cysS"
                     /locus_tag="PLM04_03050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HON02088.1"
                     /translation="MENNLYIYNTLHRKKELFVPLHAPHVGMYVCGPTVYGDPHLGHA
                     RPAITFDVLFRYLKHLGYKVRYVRNITDVGHLENDADSGEDKIEKKARLEQLEPMEIA
                     QYYANRYHKAMEALNVLSPSIEPHASSHIPEQIEFVKKILDAGFAYESEGSVYFDVEK
                     YDKKYHYGKLSGRNIDELLETTRMLEGQEEKRRSVDFALWKKASPEHIMRWKSPWSEG
                     FPGWHLECSAMSTKYLGEEFDIHGGGMDLLFPHHECEISQSTAALGKESVRYWMHNNM
                     ITINGQKMGKSLGNFITLEEFFSGNHPLLSQAYQPMTVRFFILQAHYRSTVDFSNEAL
                     QASEKGLERLMEGYDRLQKLMPSDISTSDISGLRQRCEEAMCDDLNTPIVIAHLFEAV
                     KFINLVYDGKATVSSADLEELKDVFRVFVEDLLGLKKEDENFAKYEALKKAVDLLLQI
                     RLEAKRNKDWATSDKIRNELTALGFEIKDTKDGFEWKI"
     gene            5672..6505
                     /gene="rhuM"
                     /locus_tag="PLM04_03055"
     CDS             5672..6505
                     /gene="rhuM"
                     /locus_tag="PLM04_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HON02089.1"
                     /translation="MNANNEIILYQSDNAVQLEVMLENETVWLTLNQMSILFERDKSV
                     ISRHISNIFKEQELDKNSVVAKNATTASDGKTYLIEYYNLDVIISVGYRVKSKRGTQF
                     RIWANKVLKDYLLKGYAVNQRIENLERRVSATEDKIDFFVKTALPPVQGIFYDGQIFD
                     AYKFVADLVKSATKSIVLIDNYLDESVLILLSKRKENVKCQIFTNKISAQFKLDLEKF
                     NAQYPPVEVKEFSKSHDRFLIIDETIFQIGASLKDLGKKWFAFSKINLDLQDILKKIY
                     Q"
     gene            6550..7377
                     /gene="rhuM"
                     /locus_tag="PLM04_03060"
     CDS             6550..7377
                     /gene="rhuM"
                     /locus_tag="PLM04_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HON02090.1"
                     /translation="MNNEIILYQSDNAVELEVILENETIWLTQAQIAELFGTRRQAIT
                     KHLKNIFDSGELSENSVSSKMELTAKDGKNYLTNFYNLDAILSVGYRVNSKNATQFRI
                     WANKVLKDYLLKGYAVNQRIENLERRVSATEDKIDFFVKTALPPVQGIFYDGQIFDAY
                     KFVADLVKSATKSIVLIDNYLDESVLILLSKRKENVKCQIFTNKISAQFKLDLEKFNA
                     QYPPVEVKEFSKSHDRFLIIDETIFQIGASLKDLGKKWFAFSKINLDAKLMMEMMSK"
     gene            7494..7874
                     /gene="rbfA"
                     /locus_tag="PLM04_03065"
     CDS             7494..7874
                     /gene="rbfA"
                     /locus_tag="PLM04_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HON02091.1"
                     /translation="MDNTRQQKVARLLQKELGEIFLLYAREMHGIIISVTNVRISPDL
                     SIARIHLSIFPGKQSAEVLEKINNDQKNIRFDLGKRVKHQLRIIPELFFYLDDSLDYI
                     DHIDQLLKNDSPSDKKDHSQENNQ"
     gene            7920..9209
                     /locus_tag="PLM04_03070"
     CDS             7920..9209
                     /locus_tag="PLM04_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON02092.1"
                     /translation="METIHPNQTKGDNTSGGFRFATWVARRYLFSRKSHNAINIISGI
                     AALGVGIGTMALICVLSVFNGFDSLIQGMFSTFDPDLKITVSQGKTFDINTPEWEKVK
                     KLNNVAYFTEVIEENALLRFRDKQMPATIKGVSDDFQKMTKIDSIMFDGKFLLYDGAF
                     QRAVPGVGVASILGLGANFIDPLYIYAPKRTSHINLLHPENSFNQIGTFVSGIFSVKQ
                     LQYDDHYVLVSIELARDLFEYQPNTVSSIELKLKDNIKTLDAQKKIKNILGGNFEVKD
                     RYEQQEDFFKILKIEKWITYLILCFILLIASFNIISSLSMLIIDKKQDIQTLRNLGAE
                     QQLIRRIFLFEGWLISAVGAVLGIIMGLLLCLLQQHFGILKLGTGYVVDVYPVLVNFW
                     DVLLVFMTVLLMGFLAAYYPVRYIDIHDAPSPVNVEL"
     gene            9291..9959
                     /locus_tag="PLM04_03075"
     CDS             9291..9959
                     /locus_tag="PLM04_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02093.1"
                     /translation="MKKTIKLAIALSILTFISCKDNTPNQPYQYELNPAYTWGYVVFY
                     GDYYADYLNKNNVLTVNLFTDSLKINNQNELLGYGQYLFLEDVFVAPSDTLLPEGIYR
                     VDNSGEPFTIAPGINDTIDNQVYTLGAVINYYEPNPLKSTQKLIVGGKMTVSRQGENY
                     SLNCDFVLQDSLKLKGIFSGTLPHFDESLQREERNSIYIFKENSLVKEKKTIYKFFGK
                     KKNY"
     gene            10351..10941
                     /locus_tag="PLM04_03080"
     CDS             10351..10941
                     /locus_tag="PLM04_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02094.1"
                     /translation="MNTKLQELTEKIYLEGVEKGNAEAQSIIEEAQKKADSIISSAQE
                     KAARIIAEAEAKAAELEENTKSELRLYAQQSLNALKTEITNLICGKIVDDSVKAAMVD
                     KNFMQQVILQLAKEWSKKESIVIEAPDAKALTEYFLANAKNLLNQGVTITQADKSKAT
                     FTIRPDKGGYKINFGEEELTEYFKDFLRPKLIEMLF"
     gene            11019..11876
                     /locus_tag="PLM04_03085"
     CDS             11019..11876
                     /locus_tag="PLM04_03085"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HON02095.1"
                     /translation="MNYYCLIAGLPDLSPDDKKTTFTLADFKNELLELLDTSDVEFLR
                     LIFAQYDNQNFLAFLIDRDAFLQPLGNLNYDDWVEIVQLLKETENPKDKRILPYIQHF
                     YSVYQDEEFWAEGISKEDYLTGLYYDFALQCNNSFLKSWFEFSLNINNLLTAIACRKY
                     GLNPHNYVVGSNTIAQQLRTSNARDYGLTGTFEYLEQVLQIAEEPDLMEREKKIDLLK
                     WQWMDEHTFFYYFSIEKILAYILKMQMIERWQPLTPEKGNVIFRELIEKMKQSVEFEE
                     TLQAGEKIR"
     gene            11963..13723
                     /locus_tag="PLM04_03090"
     CDS             11963..13723
                     /locus_tag="PLM04_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HON02096.1"
                     /translation="MPTKGKVKGIVSNLVTVSVDGAVSQNEICFIELGSEKLMAEVLK
                     VNGSDVFVQVFESTRGLKIDNEVTFEGHMLEVILGPGLLSKNFDGLQNDLNKLTGIFL
                     KRGERNFPLDEDKKWKFKPIAQVGDEVEAASWLGEVDENHQPHKIMVPFVMEGSYKVK
                     SIAPAGEYTISETIAIITDAHGKDFEINMIQKWPVKKAINVHAEKPRPYKLLETGVRV
                     IDTANPIVEGGTGFIPGAFGTGKTVLQHAISKQAEADIVIMAACGERANEVVELFVEF
                     PELKDPHTGRFLMERTIIVANTSNMPVAAREASVYTAMTIAEYYRAMGLRVLLMADST
                     SRWAQALREMSNRMEELPGQDAFPMDLSAIIANFYSRSGFVKLKNKQTGSITFIGTVS
                     PAGGNLKEPVTESTKKVARCYFGLEQARADSKRYPAINPIESYSKYIEYPEFDEYISE
                     RISPQWISMVNDMRTLLQRGKEVQEQINILGDDGVPVDYHITFWKSELIDFVILQQDA
                     FDDIDAVTPIERQHYMLKLIMDICKSDFNFVNFTDVMDYFKRIINVCKQMNYSEFHSQ
                     AFESYYNELQNILAERKIKQ"
     gene            13798..15129
                     /locus_tag="PLM04_03095"
     CDS             13798..15129
                     /locus_tag="PLM04_03095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HON02097.1"
                     /translation="MTTKAFQKIYTKITKITKATCSLHASGIGNDELALVNGKLAQVV
                     KILGDEVTLQVFEGTEGIPTDAEIVFLGKAPTLKVSDQLAGRFFNAFGDPIDDRPEIE
                     GEEREIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNEVMAN
                     VALRAKTDKIILGGMGLTNDDYLYFKNVFNNAGVLDRIVSFVNTTEDPPVERLLIPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALAIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPAGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDSDIGKVIVDPFR
                     SLSRLKQLVIGIKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTDYDERALAFAKD
                     YSDRLLAIDVNIDTTAMLDTAWKLFAKYFSPTEVNIKQEFIDKYWKQKQHE"
     gene            15185..15790
                     /locus_tag="PLM04_03100"
     CDS             15185..15790
                     /locus_tag="PLM04_03100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HON02098.1"
                     /translation="MSIQFQYNKTSLQQLEKNLKIRVRALPTIKNKESALRTEVKKAK
                     EEIKRIENELEQRIAEYDKMLALWGEFDTTLLEVEEVKMNIKKIAAVRVPILEEVVYK
                     TKPFSLFNSPKWYSDGLALLKKLAQIGIEQEFFIQKLSLLEFARKKTTQKVNLFEKVQ
                     IPGYEDAIRKIKRFMEDEENLSKSSQKIVKSRQELIKKENL"
     gene            15799..17622
                     /locus_tag="PLM04_03105"
     CDS             15799..17622
                     /locus_tag="PLM04_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HON02099.1"
                     /translation="MTRYTFLVYHKQYHDFLEKMREVGVVHVIEKVEGIAENDELREK
                     MLLSSRMKIALKQMEKLIPQDASPLPADLSVDGMELLKKIENKFAEKELLIQKLQLAE
                     KERDRMEMWGIFDYQLIENLQKAGYEINFFSCNVRKFDQEWEIRYNAFEIDNIGSTRY
                     FVTITHTGEIIEIDADPVKIGTKNVLQIDEDIQQIHQEIDNINRQLITLSVENYNSLK
                     AAQVKVIENMDFARVLLNTEPTVENKVMLLEGWCPEDAEEKLKEFLNETDIYYETSKP
                     EEDEKVPVKLKNNRFSRLFEFIGNLYDLPNYHELDLTPFFAPFYMLFFGICFGDAAYG
                     LLLLIASLYFRKKADAALKPILSLAALLGISTVIMGFVSGTFFGFNLLEMDVPWLASF
                     KAFMLDSNQLFYTSLLLGVVQILFGMVIKAIGKVKRYGWPASFSTWGWLILLIGCGST
                     FVLSSFAGLSPQTAKWLYIIFGSAGGALVFLLNNIKRNPLINIGSGLWDTYNTATGLL
                     GDVLSYIRLFALGISGAVMGFVFNDLAINLSGNIPIVSTLIMLVIMIFGHGINIFMCG
                     LGAFVHPMRLTFVEFYKNAGFEGGGKKYKPFSHFKEEKSII"
     gene            17691..18137
                     /locus_tag="PLM04_03110"
     CDS             17691..18137
                     /locus_tag="PLM04_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HON02100.1"
                     /translation="MEPIILAYIGIGLMVGLSGVGSAIGVTITGNATVGALKKNPDAF
                     GNYMVLSALPGTQGLYGFLGYFLLSSHLVPNMTWGASAAVFGASLALALTGLLSAIRQ
                     GQVCANGIAGIGSGYDVFGRTLILAVFPELYAIVSLAAVFMIGNAI"
     gene            complement(18273..21236)
                     /locus_tag="PLM04_03115"
     CDS             complement(18273..21236)
                     /locus_tag="PLM04_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HON02101.1"
                     /translation="MNTLSINKLYFKDTSFANLMTHRIYNILLYASKYDAFVLEEDGR
                     IDEQIFNEYTALNLRYPPRFTLVSNEEEANELLRTRTFELIISMPSGDSINPFEWAKD
                     VKKEYPEIPIVVLTPFSSSISQRLAHEDLSAIDYVFSWLGNSDILLAIIKLIEDRMNV
                     EEDVNSVGVQVILFVEDSIRWYSSIVPHLYKYVFKQSRSFMTEALNEHEQMLRMRGRP
                     KILLARSYEEAISIYNKYSQNMLGVITDVSFPEKNVKDKKAGLHLCSEIRRNDKFIPL
                     IVVSTEEENRQTAEELNAVFLNKMSKTLFMELREKITDNFWFGDLIFFNPQTGVEEAR
                     VHNLRSLQDCIFKVSDEALYYHVSRNNISKWLYSRAMFPLAEFLKNIQVEPGMSENMK
                     NVRQIIFDAIVHYRKIKNRGIVAVFQRDRFDMYSNFARIGEGSLGGKGRGLAFLDAMI
                     KRNESLDKFENVQIAIPRTVVLCTDIFSDFMEMNQLYPIALSDIPDEEILAHFLKARL
                     PQKLLADLYAFLGAVSKPIAVRSSSLLEDSHYQPFAGVYSTYMVPFDANSKTHTLQMI
                     TEAIKAVYASVFYHDSKTYMAATKNLIDEEKMAIVLQEICGSAYENRYYPSFSGVARS
                     LNYYPIGNEKPEDGIANIAMGLGKYIVDGGKTLRFSPAYPNNVLQTSTLEFMLSETQT
                     FFNALDLNMVQFVPQVDDGFNLLRINVQDAIKDGTLQYLASTYNVSDQMVYDGLYEGG
                     RKIITFANILQHNVFPLAELLREILRIAQEGMGRPVEIEFAVNLDYSPKKQHSFYLLQ
                     VRPIVDNKEIINEDISAIPEEETIVHSSNALGNGNMTDIYDLIYVKQEAFNGSNNRLI
                     MYDIEKLNRQMVKEEKYYVLIGPGRWGSSDPWLGIPVKWPNISNARVIVETELSHYRI
                     DPSQGTHFFQNLTSFGVAYFTVQPFQHLGFYDLDFLAQQPAIYESEYIRHLHFDKPLV
                     VKVDGKKNIGVIMKP"
     gene            21541..22875
                     /gene="gdhA"
                     /locus_tag="PLM04_03120"
     CDS             21541..22875
                     /gene="gdhA"
                     /locus_tag="PLM04_03120"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HON02102.1"
                     /translation="MDIEKFMQVLSAKHPNENEYLQAVREVLTTINEVYSQHPEFEKA
                     KIAERLVEPDRIFTFKVPWVDDQGNVQVNIGYRVQFNNAIGPYKGGLRFHPSVNLSIM
                     KFLGFEQIFKNALTTLPLGGAKGGSDFNPKGKSNAEVMRFCQAFMTELWRYIGPETDV
                     PAGDIGVGAREIGYLYGMYRKLAHENTGVLTGKNLEFGGSLIRPEATGFGALYFVREM
                     LQRAGMDIRGKTVAISGFGNVAWGAALKASQMGAKVVSISGPDGYVYDENGISGEKID
                     YMLELRASCNDIVSPYAEKFGVPFIPNRRPWELKVDIALPCATQNELNEEDARNLIEN
                     KVWCVAEVSNMGCTPEAIDLFIKNKIMYAPGKAVNAGGVSVSGLEMTQNAMHLSWTAE
                     EVDKQLHQIMNKIHEQCVNFGKEEDGYINYLKGANVAGFIKVARAMLAQGIV"
     gene            complement(23012..24475)
                     /locus_tag="PLM04_03125"
     CDS             complement(23012..24475)
                     /locus_tag="PLM04_03125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON02103.1"
                     /translation="MKPINCFIVYDDTVQAEITIENLLQSELVNAVYLLSKRENIQVN
                     EKCFPILIDSLYSSNTIRKIAEKSDTPYSLVYLKNTPLHLGQFALERFFQIAQDTKAG
                     MVYSDYYQVSGGVRSKLPLIDYQTGSVRDDFNFGSVLFFNAEVLKQASAQMNENFLHA
                     GLYDARLRISQKAELVHIDEYLYSEVESDNRKSGEKLFDYVDPKNRQRQLEMEHACTE
                     HLKNIGAYLKPQFKTILFDENNFEVEASVIIPVKNRATTIEDAVRSALTQKTSFPFNV
                     IVVDNHSTDGTSEIVEKLAKEDSRVRHIIPQREDLGIGGCWNVAVHSPFCGKFSVQLD
                     SDDVYQDENTLQIMVNAFYEQNCGMVIGSYTITDFEMNVIPPGLIDHREWTPENGRNN
                     ALRINGLGAPRAFYTPLLRKIKVPDSNYGEDYALGLYFSREYQIGRIFQSVYLCRRWR
                     DNSDAELDIVTLNTYNHYKDKIRTIEIKARQQINKLS"
     gene            complement(24517..26499)
                     /locus_tag="PLM04_03130"
     CDS             complement(24517..26499)
                     /locus_tag="PLM04_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HON02104.1"
                     /translation="MRTNLSSKITMTRIPRRYYQPDDVFELSRVTRLEKIPTEIFDTE
                     KEGSAKVAEEIAALIKTKQAAHEKCVLGLTSGKSMVGIYAELVRLHKKENLSFNNVVV
                     FLLFEYYPLTGKLKGSYGQLKNMLLDHVDIPAENIFYPDGTMDKHQIMQACAEYEQKI
                     AALGGIDLQLLGIGRSGNIGFNEPGSLTNSQTRLILLDTLSREDAAEFFSNAEMVPVS
                     AITLGISTILKAKRIVLVAWGEHKSTVVKNAVEGPMSETIPASYLQLHSNVKIVIDLH
                     AASELTRISMPWLVTSCEWDSKLIRRAIVWLCMKLKKPILKLTNKDYNDNGLSELLAI
                     YGSAYNVNIRIFNDLQHTITGWPGGKPNADDSNRPERALPYPKRVLIFSPHPDDDVIS
                     MGGTFKRLIDQNHKVHVAYQTSGNIAVADHEVIRFLSFVKGFKDLFDSNNDVIQAKYE
                     AMKNFLLYEKKENDMDTPEILEVKALIRRGEAKAACRYLGLSSQNIHFLNLPFYETGT
                     VKKNDLSQKDIDIIKDLLQQIQPHQIYAAGDLADPHGTHKICLDAVLAAIDELKDEEW
                     MKDCRVWMYRGAWKEWEIDHIEMAVPLSPEELKNKRNAILRHQSQMESAPYLGNDERL
                     FWQRSEERNRATAELYTQLGLASYEAIEAFVRYKPV"
     gene            26658..27509
                     /locus_tag="PLM04_03135"
     CDS             26658..27509
                     /locus_tag="PLM04_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HON02105.1"
                     /translation="MNIIADSGSTKTHWCVIEGENTVKEFYTKGINPFYQTKEEILAE
                     LKENLYPRIMQSTIDGVHFYGAGCAFAEQKKMLSEIFRDILGKELVVTIESDLLGAAR
                     SLFGKESGIACILGTGSNSCYYNGRGIEKNVPPLGFILGDEGSGAILGKKLTSDALKN
                     QLPPDLVSKFMKEYDLTLEKVLDHVYKKPFPNRYLAQFVPFIKENIQYPPLYNIVYSS
                     FNDFFNRNICQYPHSEKTPLGFVGSVAFHFADILQTIARERHFRIIDIAQNPMKGLIK
                     YHSQKNK"
     gene            27522..28346
                     /gene="murQ"
                     /locus_tag="PLM04_03140"
     CDS             27522..28346
                     /gene="murQ"
                     /locus_tag="PLM04_03140"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HON02106.1"
                     /translation="MAFEKITEQNSLHNDLDKKDIRQLLVEMNEEDKKVPFAVERAIP
                     QIEQLIEKLVERMKIGGRLFYIGAGTSGRLGVLDASEIPPTFGMPPHLVIGVIAGGDT
                     ALRNAVEFAEDDMEQGWEDLKKHNISSIDTVVGITASGTAPYVMGALRKAKQHGILTA
                     SIACNPDSPVSHEADIKIELIVGPEFVTGSTRLKSGTAQKLTLNMISTVTMIKLGRVK
                     GNKMVNMQLTNSKLIDRGTRIIVEELNIDYEQARNILLIHGSVKKAIDAYKIQNKI"
     gene            28500..31634
                     /locus_tag="PLM04_03145"
     CDS             28500..31634
                     /locus_tag="PLM04_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HON02107.1"
                     /translation="MKKILLLFAVLFLSLQVSLAQKVTVTGTVTSKEDGLPIIGASVL
                     EKGTTNGTITNYNGVYTISVPIGATLVFSYVGMDKVERKVTGEGKIDVIMNPTAIAID
                     EVIVTAMGVKAEKKKLNFAVQSVDAEAITDGRSPNFVNSLQGKISGVSVTSSGGSPNA
                     GSQILLRGISSINPSQNNEPLFILDGMVLSGGGSAAADINPSDIENVTVLKGAAASAL
                     YGQNAANGAIMITTKTAKAGKMSLSANASIQSDTPTRLLELQSIYGPGALGFYRPQES
                     GGYGGWGPLLDKDEPVYNNVKNYFQSGLYQKYDFSINGGSDKFQSYASVNYSRHDGIV
                     PKDYLDKMGLLLKGNYEINNKISANFMTNIVKNSYRGAGSISAIYSWPINDDITNYKN
                     SDGSIRYRYISIPKKENSPISPLWSRYMDYGLNSSTRNLLQGSLIYKPLKGLEITGRL
                     SLDQNNYMYDGYQVPRFDDSIILDNPPKESDYTNLDAYAQALKEYNDYYYSVPYFNEK
                     DYASVDKDFFGNYSYSQSKRELVTATLMGNYKIPLPKDFNLEVLAGTEIKTDNRISSS
                     INGRDFVIPGTYSISNVREIVGTNDVSLNHWEKRNAGVFGELRSDYKGLLNLSVTSRW
                     DWSSTIAYKYNPYFYPSVTGGIIFSEIFGLSNDWFSYGKLRGNWARVGKDVATPYLFD
                     RKYTQYPTLPDGGYGADPTMSVASNLQPEISDSWEIGLETRFFSSKTRLDVAYYSTTV
                     DNQIVTVRVSPASGYILQTRNEGNIKNHGVEISLDQDILKNKDLLWTGTLNFGFNRGK
                     VLSLPEDIVELQGTQYGDIFPTAYLNGSTTAISGKDYLRNEKGEIICTPDGYPKINPT
                     KSVLIGNREPDFLMGLTSTLKYKGISLSFLVDGRKGGDVMNVTGRSLWSSGQHKMLEF
                     YRGRQVVWDGVVEQADGTYVRNTTPIVLDNQTIINYYYNVSSNFIEDGSYIRLSYVTL
                     GYDFTNKLKNNRYIHGLRCSLTGTNLFLLTKYSGSDPQINASTSEGGTGSMGIDNYPV
                     PNTRGINFTINANF"
     gene            31649..>32993
                     /locus_tag="PLM04_03150"
     CDS             31649..>32993
                     /locus_tag="PLM04_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HON02108.1"
                     /translation="MNTTIMKGNIFKVISLILLVSIGSLLSSCEDWLDINVSKDKPVK
                     VTPDQSLPLVLLYASQLCYDHAEYGVYLSQALTTAGRSQTGSYAYKSGWEFLSMNRHP
                     QWRRHFYDIGVNAKGMIETAEEIGSKNFVLIGRTILLHSTLLTTDAFGDMPRSQVYLS
                     NSPSYDTQASIYEWMLQEADELISLYNNDEWVNNPNNKRINKNMDRMFEGDLGKWRAF
                     AKALKARILIRKLPNWENTPETCQKIIDAVDAALNDPSYTDAIYKYDGGSAEKNCPWG
                     PAQPSLNLGWPQGRQNLLSEAIPSKFFTYAILGSYSSYRATTGYALDPRAVELMTPRS
                     NDNNVKVLRYLENNIGMPATMKITFYPDLYATEGHNPYTRNNGYIMLMSREELLFIKA
                     EAQYWKGDKTAAYQTTIDAVNVNMERLGVLPTSTATKPYYDRFFSIKLPSANTFTI"
     assembly_gap    32994..33093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33094..33336
                     /locus_tag="PLM04_03155"
     CDS             <33094..33336
                     /locus_tag="PLM04_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02109.1"
                     /translation="PYNLYEAYWTTPDNYGTNAKFSPNAWITRLNYDPETEEKYNRKE
                     LQRLGAYKNPDWLKKRMIWAYKNNDKVTAADQTEWK"
     gene            33361..34218
                     /locus_tag="PLM04_03160"
     CDS             33361..34218
                     /locus_tag="PLM04_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02110.1"
                     /translation="MKNIQLKIAVLLSVFMGFFLTSCEKNDTLNDNVFIGEMAPQVYW
                     EINSSTVNAGSEVPFFVQYYTSGKEPIDRLEVWYSVEEEESKTVSCPWTQTFTFQLSS
                     TTSDNKRILQKISEYPHNESYWNDSLHAYSFNATFPTSSTLSSISWIKPATFDSTKMI
                     KYFGEGFMQHFKDSLYTKMKALDFQKMYSGLNLVENFKVYLDSTFNENSGSYDYHFKK
                     DSLGNEVIPTEIQNIYQTIPFADLIYNASNNNYDVEFSRSYQLNALIKAIDRKGTAGL
                     STKSIITLN"
     gene            34307..>35025
                     /locus_tag="PLM04_03165"
     CDS             34307..>35025
                     /locus_tag="PLM04_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HON02111.1"
                     /translation="MKTKQIIKYLGIFGLLVFFTGSCKDEMPAIEEFPSPDVTFTYSV
                     NDNSYQLDYYVGAEIKFETASATTGCTWDFGDGTNATGTTVIHKYTQAGTYQVKLTVE
                     GKGYVIQKIFISDIRPIMKLKPIEGGMCEVQTTYVSFEVELPNPEGLEEEYLWTFPEG
                     TLDENGNSRETYTGKDPGKVKFSNVGSQTVRLQVKLGGRQLEESSINVQVAFNRAVPT
                     LYYAVKTGNIMALKLSSSTPEG"
BASE COUNT        11469 a         6443 c         6721 g        10292 t
ORIGIN      
        1 cccttaaacg ggctgcttaa tataaaaaag aaagtgtcct tttgggacac cctctattta
       61 aaatatgaaa atcaaagttg aaaattaata gcctctaatg gctttgatac cgggaagaac
      121 ttttccttca atggcttcca gtaaagctcc gccacctgta gaaacataag aaacgccatc
      181 tgccaaaccg aatttgttga tgcaggcaac agaatcgcct ccaccaatga gcgaaaatgc
      241 accatttttg gtggcttcaa caatagcttg agctacagcc aaagatccgt gcgtgaaatt
      301 atcgaattca aaaacaccaa cggggccatt ccataaaatg gtttttgagg attttattac
      361 atcagcaaaa agtttctctg tttcggggcc aatgtccaaa ccttcccaat tgtcaggaat
      421 ttcgtttacg ggaacaaatt tggtattcgc atcgttgctg aaagcgtcag caatttttgc
      481 atcaacggcc aataccatat ttaccccttt gctttttgct ttttcgagca atgaaagtgc
      541 caaatccaat ttgtcttctt cgcaaattga actgcctatt tttccaccca gagcttttac
      601 aaaagtgtaa gtcataccac caccaataat cagattgtca actttattga gcagattttc
      661 aataatatca atttttgaag aaacttttga gcctcccatg atggctgtaa aaggatgttc
      721 ggtgttgttc attatttttt caacagctgc cacttccttt tccattaaat atccgaacat
      781 tttatgatcg gcatcaaaat aatcagcaat gagtgcggta gaagcatggg cacggtgagc
      841 ggttccaaag gcatcattca cgtagcaatc ggcataagaa gctagcgtag ccgtaaattt
      901 tttctgacgt tccttcaatt ctttttttgc agcagctttt tcttcttcgg tggccgtttc
      961 gggcaaacgc ggttttcctt cttcttcttc ataaaagcgc aaattttcca gcattagagc
     1021 ttctcccggt tttaaagctg aaaccatagc tgcagtttcc ggtcctatgc aatcgggtgc
     1081 aaatttaacc tctataccca aaagctttga tacatgagga agaatgtatt ttaaggaata
     1141 tttgggatcc ggatttttct tgggccttcc catgtgtgaa gcaataatta cacaaccacc
     1201 atcattcaaa atttttttaa gtgttgggat ggctgcacga atgcgcgtat cgtcggtaat
     1261 attgaaattt tcatccagtg gcacgttaaa atccacacga acaaatgcct ttttgccggc
     1321 aaaattaaaa gaatccattg tttgcatagt cttcaaatat ttaaagtggt gattattttg
     1381 taaattcaat gaaaaagaaa actgttgcaa atttagcaaa atttcgtggt ttatttaaca
     1441 aatgggaaaa gaaaagtttt aagcttgctc aggatttgat tcttatttcc catgaaatag
     1501 gaatagcttt tttgtacatg gccatgacac cacaatattt ttcgtaggaa agggtaacag
     1561 ctttttccag tttttccatc ggtaaattgt ctccttcaaa ttcgtagatt actttcatgg
     1621 atgtgtattg tttgggatgc tcttcactca tttgcgcttc tatctgaata tcaaaccctt
     1681 tcagttccac acgcattttt ttcaaaagcg aaactacatc cagtccacta caacctgccg
     1741 ctgccaccaa cagcagtttt ttgggacgtg gcccagcatc ttctcctccg gcttccttgc
     1801 ctaaatctat ggttatgatg tgcccgtcaa catcggtatc gaaaatatta tcttttctcc
     1861 aaatggtttt agttatgtgt gttgccataa tgattggtgt ttttttaagt tttttcttta
     1921 tccaacaatt ttaaacgact ttggttcgat tctctatttt tctttttatc aaattaatat
     1981 tgaattattg agcctgtacc gaattaattt tgttaatttt tatacaacag tatagaataa
     2041 gtcagaaaaa agtagtgctt gcgccgaaga aaaaataagt tctcactcca tttaatcatc
     2101 ccataaaaag ttgactattt tggtttgaaa aaacgggaag gaagtaaatt gatgtatatg
     2161 tagaaatagc ttctaccgca aacaataagt tcctcaagga aagcattttt caaagagaaa
     2221 aaatatttga ccattctgta gaaaaatgtt ttgccaatgg tgcttatgct aggaaagaga
     2281 atcaggaatt tggaatgtca atcaacaagc atcttatttt ttaagtggct ggcacgaagc
     2341 aaaagggaaa taggaacttt caaaaaattc acaaaataaa aataaaaacc aatacctaat
     2401 aaaaaaagta aaaccaagaa aaaagagcag tattaaaaat gatatattaa agacaaaaat
     2461 agccagttta aggttgagaa tgcacaaact tctcttttgc gttgagccaa aagccatgaa
     2521 acattccaaa agaagaactt atcccagatt aagcattagc caaattaata ggaaagtcaa
     2581 accaagtcgt tatttcatgg ctttgaaaca cacatccctt tttatttttt aattaaaacc
     2641 caagcatcag ggaatcggct ttgtatttta ctgatgaaaa cgtgtgcttc attgtaagta
     2701 ttaaaagaat tcataattac acgaaacatt ccttgttcgt tgattacaat aactggatta
     2761 tagccttcat tgctaagcgt acgttgtagt cctttggcat ttgattgatt tctgaaactt
     2821 cctactacca catgatattt ataattcata gcgctttgat tggtttcacc ctcagccaaa
     2881 ctgaaacttt cgcttcttgt aatttcgggg acattttcag tacttgttgt tggctgcgtt
     2941 actattacag gcgaggtggt tttttgttga ttttctgaag tcgaagtacc tgcttttgca
     3001 ttggcaccgg gaatttcaac tactttctgt ttggatttgc aagcaggcaa ggcaaatact
     3061 aatgctgaaa gaacaagaaa tgcgagaata aatttgctta atttcatgat tacttgtttt
     3121 attaaatttg ttgataaaat attttagtgt tgattgaatt tcttaaaagc ataaagttaa
     3181 acaaccagaa taacttgtag catgcaaatt tacaagatta tttttgattt ttatgaaaac
     3241 caaattaaaa aaaattaaat tgaatagata tataaattca acaaccaatc attctccttt
     3301 taagccgttg tcagctcaat tttacacgaa tgtaaatttc tatctttttt cgtatctttg
     3361 tggtttctga aatgtgtaac caattgtttt tatgagtggt tttgccaaat ggattggagg
     3421 aggcttaggc tgggctttgg gtggtccaat cggtgctttg attggttttg ccatcggctc
     3481 attcattggc gctaaaattg aaacagtaga gaaatctcaa aagagaagaa ctaccacaac
     3541 ttcaggagat ttcaaaataa gtctgttggt tttgattgct tgtatgatga aagctgatgg
     3601 acacgtcaag aaatcggaat tggaaatagt caaacgtttt ttggttgata attttggaga
     3661 agagggagca ttggaagcat tatcaatatt aaaaaatctg cttgaaaaag atattgacga
     3721 acgtcaggtt gcagaacaga tttatcgtta tacaaactat tctacacggc tggaactgat
     3781 tcatttgctt ttcgacattg catatgccga tcatcaggtt tctacttccg aactgacatt
     3841 gattgaaaga attgctggca tatttcgtat ttccggactg gattttgaat ctttaaaagc
     3901 accttatata aaacaagttg acatcaattg ggcctataaa gctcttgaaa ttgaaccttc
     3961 cgcttctgtg gatgaaatta agaaagctta ccggaaaatg gccatgaaat atcaccccga
     4021 caaggtcaac acattgggag aagatgtgaa gaaaaaggcc acagaaaaat tccgatccat
     4081 aaacgaggct tacgaaactt taaaaaagga aagaaaattt acctgaaaaa agcaagttat
     4141 caataaaaat ccaaaaaaat ttttataacc aaatggaaaa taacttatac atttacaata
     4201 ctttacacag aaaaaaggaa ctgtttgttc cacttcatgc accgcatgtg ggaatgtacg
     4261 tttgcggacc aaccgtttac ggcgatccac atttgggaca tgcacgaccg gccataacat
     4321 ttgatgtttt gtttcgctac ctgaaacatt tgggttataa ggttcgatat gtgagaaata
     4381 ttaccgacgt gggtcatttg gaaaatgatg ccgacagtgg cgaagataaa attgaaaaaa
     4441 aagcacgtct ggaacagttg gaaccaatgg aaattgctca atattatgcc aaccgttacc
     4501 ataaagctat ggaagctttg aatgttctgt ccccaagtat cgagcctcat gcttcgagtc
     4561 atatccctga acaaattgaa tttgtaaaaa aaattctcga tgcgggattc gcctatgaaa
     4621 gtgaaggatc ggtatatttc gatgttgaaa aatacgataa gaaatatcat tacggtaaac
     4681 tttcgggcag aaatatagac gaactgctgg aaacaacgcg tatgcttgaa ggacaggaag
     4741 aaaaacggcg cagcgtggat tttgctttat ggaaaaaagc atcccctgaa catatcatgc
     4801 gctggaaatc gccctggagc gaaggttttc cgggctggca tctggaatgc tcggcgatga
     4861 gtaccaaata tttgggtgaa gaatttgata ttcatggcgg aggcatggat ttgctttttc
     4921 cccatcacga atgcgaaatt tcgcagtcaa ctgctgcact cggtaaggaa agtgttcgtt
     4981 attggatgca caacaacatg attaccatca acggtcaaaa aatgggaaaa tcattaggga
     5041 acttcattac tttggaagaa tttttctccg gaaaccatcc tttgctttct caagcgtatc
     5101 agcccatgac ggttcgtttt tttattctgc aggcacatta tcgcagtacg gtcgatttca
     5161 gcaatgaagc attacaagcc tctgaaaaag gattggaacg cttaatggaa ggatatgata
     5221 gattgcaaaa gttgatgcca tccgatattt caacctccga tataagtgga cttcgacagc
     5281 gctgtgaaga agccatgtgt gatgacctga acactcctat tgttattgca catttgtttg
     5341 aagcagtgaa atttatcaat ctggtttatg atggaaaagc tactgtttcg tctgccgatt
     5401 tggaagaact gaaagacgtt tttcgagtgt ttgtggaaga cttgttggga cttaaaaaag
     5461 aagatgaaaa ttttgccaaa tacgaagcac taaaaaaagc cgttgattta ttgctgcaaa
     5521 ttcgactgga agcaaaaagg aataaggatt gggctacttc ggacaaaatc cgcaacgaat
     5581 tgactgcttt gggctttgaa ataaaggaca ccaaagatgg atttgaatgg aaaatttgat
     5641 ggaactgtat caaaatttga ctgcttagcg aatgaatgcc aacaacgaaa taattcttta
     5701 tcagtccgac aatgctgttc agcttgaagt aatgttggaa aatgaaaccg tgtggctcac
     5761 tctgaatcag atgagtatat tgtttgaaag agataaatca gttatttcaa gacacataag
     5821 taatattttt aaagaacaag agcttgataa aaattcagtt gttgcaaaaa atgcaacaac
     5881 tgcctcggat ggaaaaacat atttgattga atactataat ctggatgtaa tcatatctgt
     5941 aggttatcgt gtaaaatcca aacgtggtac tcaattcagg atatgggcaa acaaggtttt
     6001 gaaagattat ttgttgaaag gttatgcggt caaccaacgt attgaaaatt tggaaagacg
     6061 cgtttcggca acagaggata aaatcgattt ctttgttaaa acagctcttc ctccggtgca
     6121 gggaattttt tacgatgggc agatttttga tgcctacaaa tttgttgcag atttggtgaa
     6181 atcggcaaca aaatcaatcg ttttgattga caattatttg gatgaaagcg ttctgatttt
     6241 gctttcaaaa agaaaggaaa atgttaaatg tcaaattttt accaataaaa tttcagcgca
     6301 attcaagctg gatttggaaa aatttaatgc tcaatatcca cctgtcgaag tgaaggaatt
     6361 ttccaaatca catgatcgat ttttgattat cgacgaaacc atctttcaga ttggagcttc
     6421 gctgaaagat ctgggtaaaa aatggtttgc tttttcaaaa atcaatctgg acttgcagga
     6481 tatattgaag aaaatttacc aatagaatat ctcattaggt aatgaattca ataataaaaa
     6541 tttcaaaaaa tgaacaacga aataattctt tatcagtctg ataacgctgt tgagctcgaa
     6601 gtgatactgg aaaatgaaac catttggctc acgcaagctc agattgcaga attgtttgga
     6661 actcgcagac aggccattac aaaacatcta aaaaatattt ttgattccgg agaattgagt
     6721 gaaaattcag ttagttccaa aatggaacta actgccaaag atgggaaaaa ctatctcacc
     6781 aatttttata accttgatgc aatactttca gttggttata gagttaattc taaaaatgca
     6841 actcaattca ggatatgggc aaacaaggtt ttgaaagatt atttgttgaa aggttatgcg
     6901 gtcaaccaac gtattgaaaa tttggaaaga cgcgtttcgg caacagagga taaaatcgat
     6961 ttctttgtta aaacagctct tcctccggtg cagggaattt tttacgatgg gcagattttt
     7021 gatgcctaca aatttgttgc agatttggtg aaatcggcaa caaaatcaat cgttttgatt
     7081 gacaattatt tggatgaaag cgttctgatt ttgctttcaa aaagaaagga aaatgttaaa
     7141 tgtcaaattt ttaccaataa aatttcagcg caattcaagc tggatttgga aaaatttaat
     7201 gctcaatatc cacctgtcga agtgaaggaa ttttccaaat cacatgatcg atttttgatt
     7261 atcgacgaaa ccatctttca gattggagct tcgctgaaag atctgggtaa aaaatggttt
     7321 gctttttcaa aaatcaatct ggacgcaaag ctgatgatgg aaatgatgtc aaaatagaat
     7381 ataatttgaa gagacagtat aagatttggg gggttaaaca attaaaaaca gattaaaaag
     7441 gcaaaaaacg gaattgaagg ttcgtatttg cctgagaaaa aggagaattt attatggata
     7501 atacaagaca acaaaaagtg gcgcgacttc ttcagaaaga attgggagaa atttttctgc
     7561 tctatgctcg cgaaatgcac ggtatcatta ttagcgtaac caatgtccgc ataagtcccg
     7621 atctaagtat tgcgcgcatt catttgagta tttttcccgg caaacaatca gcagaagtcc
     7681 ttgaaaaaat taataacgac cagaaaaata ttcgtttcga cttgggaaaa agagtgaaac
     7741 accaactgcg aattattcct gaactgtttt tctatttgga cgattcactt gactatatcg
     7801 atcatattga tcaactttta aaaaatgatt ctccatccga taaaaaggac cattcgcaag
     7861 aaaacaatca atgattattg aaaattggat aatgaaagca gattttgccc gaagaaacaa
     7921 tggaaacaat tcatccaaac caaaccaaag gagataacac atcaggagga tttcgttttg
     7981 ctacatgggt ggcacggcga tatttgtttt cacgcaaatc gcacaatgcc atcaatataa
     8041 tatccggaat tgcagctttg ggggttggca ttggaactat ggcacttatt tgtgtccttt
     8101 ccgttttcaa tggtttcgat tccttgatac aaggcatgtt ttcgactttc gacccggatt
     8161 tgaaaattac tgtttcgcaa ggaaaaactt tcgatataaa tactccggaa tgggaaaaag
     8221 tgaaaaagtt gaacaacgtt gcctatttta cggaagttat tgaagaaaat gctcttctgc
     8281 gatttcgaga caagcaaatg cccgccacca ttaagggcgt aagcgacgat tttcaaaaaa
     8341 tgactaaaat agacagcatt atgttcgatg gaaagttttt actttatgat ggggctttcc
     8401 aaagagccgt ccccggcgtc ggcgttgcaa gcattttggg attgggagcc aattttatcg
     8461 acccattgta tatttacgcc ccaaaacgca cttcacacat taatttgctc catcctgaaa
     8521 acagttttaa ccaaatcgga acatttgtat caggaatttt ttcagttaag caattgcaat
     8581 atgatgatca ttatgtgctg gtttctattg agctggctcg tgatttgttc gaatatcaac
     8641 caaatacagt ctcatcaatt gaattaaagc taaaagataa tatcaaaact ttagatgcac
     8701 agaaaaaaat aaaaaatata ctgggaggta attttgaagt taaagacaga tatgaacagc
     8761 aggaagattt ttttaaaatc ctgaaaatcg aaaaatggat tacttatctc attttatgtt
     8821 ttattttgct gatagccagt ttcaacatta tcagttcgtt atccatgttg attattgaca
     8881 aaaaacagga cattcaaacc ctgcgaaatt taggagctga gcagcaactg ataagaagaa
     8941 ttttcctttt tgaaggatgg ctaatttcgg cggttggtgc tgttctggga attataatgg
     9001 gattgttgct ctgtttactg cagcaacatt ttggcatttt aaaactcgga accggttatg
     9061 tagtggatgt ttatcctgtt ttggttaatt tttgggatgt tttgctggta tttatgactg
     9121 ttttgttaat gggttttctg gcagcctact atccggttcg ttacattgat atacatgatg
     9181 ccccttcgcc tgtgaatgta gaattataat ttatcgagaa gtgctatgaa agtttgttta
     9241 taaagccagc tgttttatag atttcatcaa tatatactaa tcaaaaaaac atgaaaaaaa
     9301 cgattaaatt ggcaattgcc ttaagcattt tgacttttat ctcctgtaag gacaacacac
     9361 ccaatcaacc ttatcaatac gagctgaatc cggcttatac gtggggctat gtcgtttttt
     9421 acggagatta ctatgccgat tatttgaata agaacaacgt tttgacagtc aatttgttca
     9481 cagattcgtt aaaaataaac aaccaaaatg aacttttggg ttatggtcaa tatctttttc
     9541 ttgaagatgt ttttgtagct ccttccgata cgcttcttcc cgaaggaatt tacagagttg
     9601 ataacagtgg cgaacctttc accattgctc ccggaataaa tgatacaatt gacaaccagg
     9661 tctatacgtt aggagctgtc attaattatt atgagcccaa tccattgaaa tccacacaaa
     9721 aacttattgt tggcggaaag atgacggtca gccgtcaggg tgaaaattat tcactgaatt
     9781 gtgatttcgt tctgcaagat tccctgaaac tgaaaggaat tttttccggc actcttcctc
     9841 atttcgacga atcattgcag agagaggaaa gaaattctat ttatattttc aaggaaaatt
     9901 ctttagtaaa ggaaaagaaa acaatctata agttctttgg gaaaaagaag aattattagc
     9961 aacctaaata aattaaattc ggttgacttt acttttacat tgtttattca tcatttaatt
    10021 ttacaaatca tatttttaaa aatctaaccc tatttttata aaaaccggat agttgtaaat
    10081 tcggactgtt gtttagtgtg cagttactct tcgctcgaga ataaatttgt ttaaaatttt
    10141 cgaggtaaca cattatgcag ttaattgctc gtaaaatttt cttttttaac taaaagaccc
    10201 tgcaacaagc ctcaaaaaat atttttttaa acaattctgg aatataaaat ggaatatgcg
    10261 ttttcatttt ttaaattttc ctaaaaacgt tttgatttag acagaaaaga ttaaatttgc
    10321 aatataaaac atcagaaaaa tactttgtat atgaatacta aactgcagga attgaccgaa
    10381 aaaatttatt tggaaggagt agaaaaaggg aatgctgaag cacagtctat tattgaagaa
    10441 gctcaaaaga aagcagattc gatcatttcc tccgctcaag aaaaagctgc gcgcataata
    10501 gccgaagccg aagcaaaagc tgctgagctc gaagaaaata ccaaatctga actcaggcta
    10561 tatgcacaac aatcactcaa tgcactaaag accgaaatta ccaatctgat ttgcggaaaa
    10621 atagttgacg attcagtgaa ggcagctatg gtcgataaaa atttcatgca acaagttatt
    10681 cttcaacttg ccaaagaatg gtctaaaaaa gaatctatag ttattgaagc tcccgatgcc
    10741 aaagctttaa cagaatattt cttagccaat gcaaagaatt tgctgaatca gggtgttacc
    10801 ataacccaag ccgacaaatc aaaagcaacc tttactattc gaccggacaa gggaggatac
    10861 aaaataaatt tcggagaaga agagttgaca gaatatttca aggatttttt acgccccaaa
    10921 ctaatagaaa tgctttttta aacaagatga tgataccgta ttttttcaaa aaaacatcaa
    10981 gaaaaaatac gtaaattcaa gaatcaaaaa agaaagttat gaattattac tgtcttattg
    11041 ccggacttcc cgacttgtct cccgatgata agaaaactac atttactctc gctgatttca
    11101 aaaacgaact tttggaattg cttgatactt cggatgtaga atttctgaga ttgatttttg
    11161 cccaatacga caatcaaaac tttctggctt tcctcataga cagggatgct ttcctccaac
    11221 ctttaggaaa tttgaattac gacgattggg tagaaatcgt tcagttgctg aaagaaacag
    11281 aaaaccccaa agacaaaaga atattgcctt acatccagca tttttattcg gtgtatcagg
    11341 atgaagaatt ctgggcggaa ggcatttcta aagaagatta cctcactgga ttatactatg
    11401 attttgcctt gcaatgcaac aacagttttt taaaatcatg gtttgaattc agtttgaata
    11461 tcaataattt gcttactgcc attgcctgta gaaaatatgg actgaatccc cacaattacg
    11521 ttgtaggcag caacacaata gctcaacaac tgagaacaag caatgctcgc gattatggtt
    11581 tgaccggcac attcgagtac cttgaacagg ttttgcaaat tgcagaagaa cccgatttaa
    11641 tggaaagaga gaaaaaaatt gatcttttaa aatggcagtg gatggatgaa cacacatttt
    11701 tttattattt ttccatcgaa aaaatattgg cctacattct gaaaatgcaa atgatagaac
    11761 gctggcaacc attgactccc gaaaagggaa atgtaatttt cagggagttg atagaaaaaa
    11821 tgaaacaaag tgtagaattt gaagaaactc ttcaggccgg agaaaaaatc agataattct
    11881 aataagtaaa atatgtttaa ttaaaaagac ctctctttgt tgaaagttca aaagagaaaa
    11941 aataaggaaa caacaaacaa ctatgccaac aaaaggaaaa gtaaaaggaa tcgtttctaa
    12001 cttggtaacc gtaagtgttg acggagcagt atcacaaaat gaaatttgtt tcatagaact
    12061 gggttcggaa aagcttatgg ctgaagtgct gaaagtaaat ggttctgatg tttttgtgca
    12121 agtatttgaa agcactcgcg gattaaaaat tgacaatgaa gtaacatttg aaggtcacat
    12181 gttagaagta atactggggc ccggcttgct gtcgaaaaat ttcgacggtc ttcaaaatga
    12241 cttaaacaaa ctaacaggta ttttccttaa acgtggagaa cgaaattttc cactggacga
    12301 agataaaaaa tggaagttta agcctattgc tcaggtaggg gatgaagttg aagctgcttc
    12361 atggttgggg gaagtcgacg aaaaccatca accgcacaaa attatggtac cctttgtcat
    12421 ggaaggcagt tataaggtaa aaagcattgc cccggcaggc gaatatacga tatctgaaac
    12481 gatagccata attaccgatg cacatggaaa ggattttgaa ataaatatga tacaaaaatg
    12541 gcctgtaaaa aaggcaataa atgtacacgc cgaaaaaccg agaccctata aattgctcga
    12601 aacaggtgtt cgcgtcatag acacagccaa cccaattgta gaagggggaa caggatttat
    12661 tcccggtgct tttggaacag gaaaaaccgt tttgcaacac gccatttcca aacaagccga
    12721 agcagacatt gttatcatgg ctgcttgtgg tgaacgtgca aatgaagtag ttgaattgtt
    12781 tgtagaattt cccgaattga aagatccaca cacaggtcga tttttaatgg aacgtaccat
    12841 tattgtggcc aacacttcaa atatgcctgt tgccgcccgt gaagcatcag tttacacggc
    12901 catgacaata gctgaatatt acagagcaat gggacttcga gtgttattga tggcagactc
    12961 tacttcacgc tgggctcagg ctttacgcga aatgagtaac cgcatggaag aattaccggg
    13021 acaagatgct tttccgatgg atttgtcggc tattatagcc aatttttaca gccgatcggg
    13081 ttttgtcaaa ttaaaaaaca aacaaaccgg ttccatcacc tttattggaa cagtatcgcc
    13141 ggctggaggt aatctgaaag aacctgtaac ggaaagcacc aaaaaagtag cccggtgtta
    13201 ttttggactt gaacaggcaa gagccgatag taaaagatat ccggccatca atcccattga
    13261 aagttattca aaatatattg aatatcccga atttgacgaa tacatctcgg agcgtatttc
    13321 tccccaatgg atcagtatgg taaacgacat gcgaacactt cttcaacgcg gtaaagaagt
    13381 tcaggaacaa atcaatattc tgggagatga tggtgttccc gtcgattatc acattacttt
    13441 ctggaaatcc gaattgatag actttgttat tcttcagcaa gatgcctttg acgatataga
    13501 tgccgtaact ccgatagaaa gacagcatta catgttaaaa ctgattatgg atatctgcaa
    13561 atcagatttt aactttgtaa attttaccga tgttatggat tatttcaaac gaattattaa
    13621 tgtttgcaaa caaatgaact attccgaatt tcattcccaa gctttcgaaa gttattacaa
    13681 tgaactgcaa aatatacttg cagaaaggaa gataaaacaa taattttttt tgacaaatat
    13741 aaagactaaa aagaaagaaa tttcccggaa aaagagaaaa taataatctg agaaaaaatg
    13801 acaacaaaag catttcaaaa aatttatacc aaaattacca aaatcacaaa agcaacctgt
    13861 tctcttcatg caagtggcat tggcaatgac gaactggctt tagtaaacgg taaattggct
    13921 caagtagtta aaatcttagg cgatgaagta accttgcaag tttttgaagg aactgaagga
    13981 ataccaaccg atgcagaaat tgtctttctc gggaaagcac caaccctaaa agtaagcgat
    14041 caactggcag gccgtttttt caatgctttt ggagacccta ttgatgaccg ccccgaaatt
    14101 gaaggtgaag aaagagaaat tggaggtcct tcagttaatc ccgtacgacg gaaacaacct
    14161 tcggaactca ttgcaacggg aattgcgggc atcgacctga ataacacgct ggtatccggt
    14221 cagaaaatac cattttttgc cgatcccgat caaccataca atgaagtaat ggcaaatgtt
    14281 gccttgcgtg caaaaaccga taaaattatt ttagggggaa tgggacttac aaatgatgat
    14341 tatctctatt tcaaaaacgt tttcaacaat gccggcgtac tagaccggat tgtcagtttc
    14401 gtgaatacca cagaagaccc tccggtagag cgtttactta ttcccgatat ggcacttacc
    14461 gctgcagaat atttcgctgt cgacaagaat gaaaaagtat tggttttgct caccgatatg
    14521 acattatatg ccgatgcatt ggctatagta tccaatagaa tggatcagat tccttctaaa
    14581 gattccatgc cgggctcact gtattccgac ttggcaaaaa tatatgaaaa agccgtacaa
    14641 tttcccgccg gaggttctat taccattatc gcggtaacta ctctctcggg aggcgacatt
    14701 acccatgcta ttcccgacaa tacaggttat attaccgaag gacaattgtt cctgcgtcgc
    14761 gattcggata taggcaaagt aattgttgac cccttccgtt ccttgtcgcg cctcaaacag
    14821 ctggttatcg gcataaaaac tcgcgaagat cacccacagg taatgaacgc agctgttcga
    14881 ctttatgctg atgctgccaa tgccaaaaca aaacttgaaa atggatttga tctcaccgac
    14941 tatgacgaaa gagcattggc ttttgccaaa gactattccg acagattatt ggctatcgat
    15001 gtcaatatag acacaaccgc catgctcgac acggcatgga aactctttgc caaatatttc
    15061 agcccaacag aggtgaacat taaacaggaa ttcatagata aatattggaa gcaaaaacag
    15121 catgaataaa tcaacatttg ttaaattgct aaaaaaaatt atcatttagt taaataaacg
    15181 ttttatgtcc atacaatttc aatataataa aacatcgctg caacagcttg aaaaaaattt
    15241 aaaaataaga gtgcgcgctt tacccacaat taaaaacaaa gaaagcgctt tacgtaccga
    15301 agtgaaaaaa gctaaggaag agataaaaag aattgaaaat gaattggagc aacgtattgc
    15361 agaatacgat aaaatgcttg ctctatgggg cgaatttgat actactttat tggaggtaga
    15421 agaagtaaag atgaacataa aaaaaatagc agctgtacga gtaccgatat tggaagaagt
    15481 agtttataaa accaaacctt tcagcctatt taattctcca aaatggtatt ccgatggatt
    15541 ggcccttctt aaaaaactgg ctcaaatcgg aatagagcag gaatttttca tacaaaagct
    15601 aagcctgctg gagtttgctc gaaaaaaaac cacccaaaaa gtcaatttgt ttgaaaaagt
    15661 acaaattccc ggctacgagg atgctattcg taaaatcaaa cgtttcatgg aagatgaaga
    15721 aaatttatcc aaatcgtctc aaaaaatagt taaatcgcgt caggaactta taaagaagga
    15781 aaatttatga ttttgcaaat gacacgatat acttttctgg tgtatcacaa acaataccac
    15841 gatttcctcg aaaaaatgag ggaagtgggt gttgttcacg taattgaaaa agtggaaggc
    15901 attgccgaaa acgacgaatt acgtgaaaaa atgctacttt cttcgcgcat gaaaatagct
    15961 ctcaaacaaa tggaaaaatt gatacctcaa gatgcttctc ctctaccggc cgatctatcg
    16021 gttgatggaa tggaactttt gaaaaaaatt gaaaataaat ttgcggaaaa agaattatta
    16081 attcaaaaac tccaacttgc tgaaaaagaa cgagaccgca tggaaatgtg gggaattttt
    16141 gattatcaac ttatagaaaa tctgcaaaag gcaggttacg aaattaattt cttcagttgt
    16201 aatgtgcgaa aattcgatca ggaatgggaa atacgctaca atgcttttga aatcgataat
    16261 attggcagca cacgttattt tgtaaccata actcatacag gcgaaattat tgaaatagat
    16321 gctgatcccg taaaaatcgg cacaaaaaat gtattgcaaa tagatgaaga tattcaacaa
    16381 atacatcaag aaattgataa cattaatcgc caactcataa cactttctgt tgaaaattac
    16441 aactcattaa aagcagctca agtaaaagtt attgaaaata tggattttgc aagagtgttg
    16501 cttaacacag agccaactgt ggagaacaaa gtaatgcttt tggaaggctg gtgtccggaa
    16561 gatgcagaag aaaagctcaa agaatttctt aatgaaacgg atatttacta cgaaaccagc
    16621 aaacctgaag aggacgaaaa agtacccgta aaattgaaaa acaatcgctt ttcgcgcttg
    16681 tttgaattta taggcaacct ctatgactta cctaattatc atgaattaga cctgacccct
    16741 ttcttcgcac cattttacat gctttttttt ggaatttgct ttggcgatgc agcgtacgga
    16801 cttcttttac tgattgcatc tctttatttc agaaaaaaag cagatgcagc actaaaacca
    16861 atattgagtt tggctgccct attaggaatt tctaccgtta ttatgggctt tgtcagtgga
    16921 actttttttg gatttaactt actggaaatg gatgtcccct ggctcgcatc ttttaaagca
    16981 tttatgttag attccaatca attgttttac acatcgctac tgcttggcgt ggttcaaata
    17041 ctttttggga tggtaattaa agcaattgga aaagttaaac gttatggctg gcctgcttcc
    17101 ttttctacgt ggggatggct tattttgttg atcggttgcg gaagtacttt tgtcctttcg
    17161 tcatttgcag ggctttcgcc ccaaactgcc aaatggttgt atataatttt tggcagtgca
    17221 ggtggagctt tggtatttct gctgaacaat ataaaaagaa atccacttat caatatagga
    17281 tcagggttat gggatactta taacactgct accggcttac tgggagatgt actgtcttat
    17341 atcagattat ttgcactagg tatttctggt gcagttatgg gttttgtttt caacgatctt
    17401 gccatcaatt taagcggcaa catacctata gtgagcacac tcataatgtt ggttatcatg
    17461 atttttggac atggaatcaa catatttatg tgtggtctgg gtgctttcgt tcatcccatg
    17521 cgtcttactt ttgtagaatt ttataaaaat gcaggcttcg agggcggcgg gaaaaaatat
    17581 aaaccttttt cgcactttaa agaagagaaa agcatcatat aatttttcgg aataaaatcc
    17641 aactaaaaaa agaaatatca catttaaaaa acaaatttaa aaaaaaaatt atggaaccta
    17701 ttattttagc ttacattggt attggcctaa tggtgggact ttcaggagta ggaagtgcta
    17761 ttggcgtaac gattaccggc aatgccactg tcggtgcttt gaaaaaaaat cccgatgcat
    17821 ttggaaacta tatggtttta agtgcattac caggtacaca aggattatat ggatttcttg
    17881 gatatttttt gctctcatca catttagttc ccaacatgac gtggggtgct tcagcagcag
    17941 tatttggcgc cagtttggca cttgccctta cagggcttct ttctgccata cgacaaggtc
    18001 aagtttgtgc aaatggtata gccggtattg gatcaggata tgacgtcttt ggtcgaacat
    18061 taatactggc agtatttcct gaactttatg ccattgtttc attggcagct gtttttatga
    18121 ttggaaatgc tatctgatat tccaatctga aaaaatatct ctgaaacaca ataaaacaac
    18181 ctcaaatcaa ttattttttc tcattgtgaa catcgaacag gatttttgtc ttgttcgatg
    18241 ttttcattag gtttagcatt tcagaaaaaa ttttacggtt tcatgatcac gccgatattc
    18301 tttttcccat ccactttcac caccagcggc ttatcgaaat gcaaatgacg aatgtattcc
    18361 gattcgtata ttgccggttg ctgagccaag aaatcgaggt cataaaaacc gagatgctga
    18421 aaaggttgta ccgtaaaata ggctacacca aaagaagtca gattttggaa aaaatgcgtt
    18481 ccctgactgg ggtcgattcg ataatgcgat aattccgttt ccacaattac tctggcattg
    18541 ctaatgttgg gccatttcac cggaataccc agccaagggt cgcttgagcc ccatcgtccc
    18601 ggacctatca acacataata tttttcttct ttaaccattt gccggttcaa tttttcaatg
    18661 tcgtacatga tcaaccgatt atttgagcca ttgaaagctt cctgttttac gtaaatcaaa
    18721 tcgtaaatat cggtcatatt gccgttgccc aaagcattgg aactgtgaac aatagtttct
    18781 tcttcgggaa tagcactgat atcttcattg ataatttcct tgttgtcgac tatcggccta
    18841 acctgcaaca aataaaacga atgttgcttt ttgggtgaat aatccaaatt tacggcaaat
    18901 tcaatttcaa ccggtcgtcc catgccttcc tgcgcaatcc gcaaaatttc tcgcaacaat
    18961 tcggccaaag gaaaaacatt gtgttgcaat atgttggcaa aagttatgat ttttcttcca
    19021 ccttcatata agccatcata caccatctga tccgacacat tgtaggttga agccagatat
    19081 tgaagtgtcc cgtctttaat ggcatcctgc acgttgatac gcaaaaggtt gaagccatca
    19141 tcaacctgag gaacgaactg taccatattc aaatccaatg cgttgaaaaa cgtctgtgtt
    19201 tcgcttaaca taaattccag cgtactggtt tgcaaaacat tattgggata ggcgggagaa
    19261 aatcgaagag ttttgcctcc atcgacaatg tattttccca atcccattgc aatattggca
    19321 attccatctt caggcttttc atttccaatt ggataatagt tcaacgaacg ggctactccc
    19381 gaaaaagaag gataataacg attttcgtaa gcgctgccac agatttcctg caacacaatg
    19441 gccatttttt cttcatcgat caaattcttg gtggctgcca tgtaagtttt actgtcgtga
    19501 taaaaaaccg aggcataaac agctttaatg gcttccgtta tcatttgcag tgtatgagtt
    19561 ttgctgttgg catcaaaagg taccatgtag gttgaataaa ctcctgcaaa aggctgataa
    19621 tgagaatctt ccaacaaact tgaggatctt acggcaatgg gtttgcttac agctcccaaa
    19681 aaagcataaa gatcggctaa aagtttctgt ggcaatcgag cttttaaaaa atgtgccaga
    19741 atttcttcgt cgggaatatc ggacaaagca atgggataga gctgattcat ttccatgaag
    19801 tcggaaaaaa tatcggtgca cagtacgacc gttctgggaa tggcaatctg aacattttcg
    19861 aacttatcga gactttcgtt gcgtttgatc atggcatcca aaaaagccaa ccctctcccc
    19921 ttgcctccca atgaaccttc gccaatgcgg gcaaaattag aatacatgtc gaacctgtcg
    19981 cgttgaaaaa cagctacgat tccacgattt tttatttttc ggtaatgaac aatggcatca
    20041 aaaataattt gacgtacatt tttcatgttt tcggacattc cgggttcaac ttgaatattt
    20101 ttcagaaatt ctgccaacgg gaacatagct ctcgaataca accatttgga gatattgtta
    20161 cgagaaacat ggtaatacaa agcttcgtcg ctcaccttaa aaatacagtc ctgtaaactt
    20221 ctcagattgt gaacgcgtgc ttcttccacc ccggtctgag gattaaaaaa tatcaggtcg
    20281 ccaaaccaaa aattatcggt gattttttcc cgcaactcca taaaaagcgt tttcgacatt
    20341 ttgttcagaa aaacagcatt caattcttct gcggtttgtc gattttcttc ttcagtagaa
    20401 accacaatca gcggaataaa tttgtcattt ctccgtattt cgctgcataa atgtagaccg
    20461 gcttttttgt ctttgacatt tttttccgga aaacttacat ccgtaatcac gccaagcata
    20521 ttctggctat atttgttata aatggaaata gcttcttcat aagagcgtgc caacaaaatt
    20581 tttgggcgtc ccctcatgcg taacatttgc tcatgttcgt tcaaagcctc agtcataaat
    20641 gaacgcgact gcttaaaaac gtatttgtat aaatgaggaa caatggatga ataccagcgt
    20701 atggagtcct caacaaaaag aataacctga acaccaaccg aattgacatc ttcttccaca
    20761 ttcattctgt cttcaatcag tttgatgata gccaacaaaa tatctgaatt acccaaccaa
    20821 ctgaaaacat aatcgatggc actcaaatcc tcgtgtgcca atcgttgaga aatggatgaa
    20881 gaaaaaggag taagcaccac aatagggatt tcgggatatt ctttttttac atcttttgcc
    20941 cattcaaaag gatttatact atcgccactc ggcatggaaa taatcagttc aaaagtacgg
    21001 gtacgaagca actcattggc ctcctcctca ttcgaaacaa gtgtaaaacg gggcggataa
    21061 cgcaaattca aagctgtata ttcgttgaaa atttgttcat caatgcgtcc gtcttcttcc
    21121 agcacaaaag catcgtattt actggcataa agtaaaatat tataaatccg atgcgtcatt
    21181 aagttggcaa aagaagtgtc tttaaaatat aacttattaa tacttaaagt gttcataaat
    21241 aaatgttttc tgttattttg aagccaatga atctcaataa aaaaatcaac tgaacttcaa
    21301 aacacaagtt tatcgaaata ttttttcaaa attagctcaa aaagtttgat aatcccaaat
    21361 aaaaatttta aacttgcaaa gaaataaaat gacagaaaaa tatcgaatga aatcaaaatt
    21421 ttcaagaaaa accaacaact ttatgcttgt caaccaagaa ataaatgaca atatgtaaaa
    21481 attttcaaaa atttgagcta cacaaatcta tatttcaaca aaaaactaaa atctaatatt
    21541 atggacatag aaaaatttat gcaggttctt tcggcgaaac atcccaacga aaacgaatat
    21601 ttacaggccg taagagaagt gcttaccacc attaacgaag tgtattctca acatcccgaa
    21661 tttgaaaagg caaaaattgc agaaaggttg gttgaacctg accgaatttt tacttttaaa
    21721 gtcccttggg ttgacgatca ggggaatgtt caggtcaaca ttgggtatcg tgtgcaattc
    21781 aacaatgcca tcggacctta caaaggcggt ttgcgttttc acccttccgt gaatctttcc
    21841 atcatgaaat ttcttggttt tgaacaaata tttaaaaatg cgctgacaac tttacctctg
    21901 ggaggtgcca aaggaggttc cgatttcaat ccaaaaggaa aatcgaatgc tgaagtgatg
    21961 cgtttctgtc aagctttcat gaccgaattg tggcgatata taggccccga aacggatgtg
    22021 ccggcaggag acattggtgt tggagctcgt gaaataggct atttgtatgg aatgtatcgc
    22081 aaacttgccc atgaaaatac gggagtttta accggaaaaa atttggaatt tggcggctct
    22141 ctaattcgtc cagaagcaac cggatttgga gctctttatt ttgtaagaga aatgcttcaa
    22201 agagcaggta tggatataag gggaaaaacc gtagccatct ccggttttgg aaatgtggca
    22261 tggggagctg cgctgaaagc atcacaaatg ggagcaaaag ttgtttcaat ctcgggcccg
    22321 gatggttatg tatatgacga aaacggtatt tccggcgaaa aaattgatta tatgttggag
    22381 cttcgggcaa gttgtaacga cattgtttct ccttatgccg aaaagtttgg cgttccattt
    22441 ataccaaatc gacgtccgtg ggaattaaaa gtggacattg ccctgccttg tgccacacaa
    22501 aacgaactga acgaagaaga tgcgcggaat ctgattgaaa ataaggtttg gtgtgtggcc
    22561 gaagtttcca acatgggttg tacacccgaa gccattgatt tatttatcaa aaacaaaata
    22621 atgtatgctc caggaaaagc tgtaaatgcc ggtggcgtgt cggtttcggg tttggaaatg
    22681 acacaaaatg ccatgcacct ttcttggacg gcagaagaag tggacaaaca attgcatcaa
    22741 atcatgaaca aaattcacga gcaatgcgtt aactttggaa aagaagaaga cggatatatt
    22801 aattacctga aaggagcaaa tgttgcagga ttcataaaag ttgccagggc catgctggct
    22861 cagggaatcg tttaattttt ctacatgttt acaattcaaa aaacgaaagg aaattatttt
    22921 ttctgtttgt gaaataatga tttcgaaaaa ctaaaaagga ggaaaatcat ttttgatctc
    22981 tcctcctttt tgcattaaga attttcattt ttcaggacaa tttattgatt tgctgccgag
    23041 ctttgatttc tatggttcta attttatcct tataatgatt gtacgtgttc aaagtaacaa
    23101 tatcaagctc agcatcagaa ttatcgcgcc aacgacggca aagataaacc gattgaaaaa
    23161 ttctgccaat ttgatattca cggctaaaat acagtcctaa agcataatct tcaccataat
    23221 tggaatcggg gactttaatt tttctcagta gcggtgtata aaaagctctt ggtgcaccca
    23281 acccgttaat tctcagggca ttgtttcttc cgttttcagg agtccattcc ctgtgatcaa
    23341 tcaagcccgg cggtatcaca ttcatttcaa aatctgttat ggtataagaa ccgataacca
    23401 taccacaatt ttgctcgtaa aaagcattta ccattatttg aagcgtattt tcatcctgat
    23461 aaacatcatc actatccaac tgaacggaga attttccgca aaaaggagaa tgaacagcaa
    23521 cattccaaca accgccaata cccaaatcct cccgttgagg aattatatgc cgcactcgac
    23581 tgtcttcttt tgccaatttt tccacaattt ccgaagtccc atcggtagaa tgattatcca
    23641 ccacaatcac attgaaaggg aaagacgttt tttgagtcaa agccgaacga acggcatctt
    23701 caatggtggt tgccctgttc tttactggga tgattacgga agcttccacc tcaaaattgt
    23761 tttcatcgaa aagaattgtt ttaaactgtg gcttgagata ggcaccgata tttttcaaat
    23821 gttcggtgca ggcatgctcc atttccaact gacgctgacg atttttcgga tcaacataat
    23881 caaacaactt ttctccactt tttcgattat ccgactctac ctccgaatac aaatactcat
    23941 cgatatgaac caattcggct ttctgtgaaa ttcttaatct ggcatcatat aaaccggcat
    24001 gaagaaaatt ttcgttcatt tgggcagagg cctgcttcaa cacctccgca ttaaaaaaca
    24061 aaacggagcc aaaattaaaa tcgtcgcgaa cgcttccggt ctgataatcg atcaacggta
    24121 atttgctgcg aacgcctccc gacacttgat aataatcgga ataaaccatt cccgcctttg
    24181 tatcctgagc aatttgaaaa aaacgttcca aagcaaactg tcccagatgt aaaggagtat
    24241 ttttcaaata aacaagtgaa taaggcgtat cggatttttc agcaattttc ctaattgtat
    24301 tgctgctgta aagcgaatcg atcaaaatcg gaaaacattt ttcgttgacc tgaatatttt
    24361 ctcgcttgga aagcaaatac acagcattaa ccaattccga ctgcaacaaa ttttctatgg
    24421 taatttcagc ctgaacagta tcatcgtaaa caataaaaca attaatgggt ttcataacaa
    24481 atatttaata agttgatttg taacgcagac aaataattat actggcttat accggacaaa
    24541 ggcttcaatg gcttcataag aggccaaacc caactgggtg tacaattcgg cagtagcccg
    24601 attcctttct tccgaacgtt gccagaaaag tctctcatcg tttcccaaat agggagcact
    24661 ttccatttgg gattgatgac gcaaaatagc atttcgcttg tttttcaact cttccggact
    24721 taagggaaca gccatttcga tatgatctat ttcccattct ttccatgcac cacgatacat
    24781 ccaaactcgg cagtctttca tccattcttc atctttcagt tcgtcgatag cggccaatac
    24841 ggcatccaag caaatcttgt gagttccgtg agggtcggcc aaatcaccgg ctgcgtaaat
    24901 ctgatggggt tgaatctgct gcagcaagtc tttaataatg tcgatatcct tttgcgataa
    24961 atcgtttttc tttactgttc ccgtttcata aaagggaaga ttaagaaaat gaatgttttg
    25021 agagcttaaa cccaaatatc tacaagctgc ctttgcttct ccccgcctga ttaatgcttt
    25081 tacttcgaga atttctggtg tatccatatc attttccttt ttttcgtaaa ggagaaaatt
    25141 cttcattgct tcatatttgg cctgaatcac atcattattg ctgtcgaata aatctttaaa
    25201 tcctttcaca aaagaaagga agcgaatgac ttcatgatcg gcaactgcga tatttccgga
    25261 tgtttgataa gctacatgca ctttatgatt ttggtctatt aaccttttga aagttccacc
    25321 cattgaaata acatcatcat cgggatgcgg gctaaaaatg agaacacgtt tgggataagg
    25381 aagtgccctt tcgggacgat tagaatcatc agcatttggt tttccaccgg gccatcctgt
    25441 aatggtgtgc tgtaaatcgt taaaaattcg gatatttaca ttgtaggcag agccataaat
    25501 agccaataat tcacttaatc cattgtcgtt gtagtcctta ttagtcaatt tcagaatcgg
    25561 ttttttcaac ttcatgcaaa gccaaacaat ggcacgacgt atcagtttgc tgtcccactc
    25621 acaggatgtt accagccaag gcatgctgat gcgcgtcaat tcactggcag catgaagatc
    25681 aataactatt tttacattgg aatgaagttg aagataggat gcaggaatgg tttcggacat
    25741 ggggccttct actgcatttt ttacgaccgt agatttatgt tcgccccatg caaccaaaac
    25801 aattcttttt gctttaagaa tagttgaaat tcccaaagta atggcagaaa caggaaccat
    25861 ttcggcattg ctgaaaaatt cagcagcatc ttcccttgaa agcgtatcga gcaaaataag
    25921 gcgggtttga gagttagtca gtgaaccggg ctcattgaaa ccaatattgc ctgagcgccc
    25981 gattcctaaa agctgtaaat cgataccgcc caaagcagca attttttgct catattctgc
    26041 acaagcttgc atgatttgat gtttgtccat tgtgccatcc ggataaaaaa tattttctgc
    26101 cggaatatca acatgatcca acagcatgtt ttttaattgt ccataacttc cttttaattt
    26161 gcccgtgagc ggatagtatt caaacaataa aaaaacaaca acattattaa aactcaagtt
    26221 ttcctttttg tgcaatcgaa ccagttcagc ataaattccc accatcgatt tacccgaagt
    26281 aagacctaaa acacacttct catgggctgc ctgttttgtt ttaattaagg ctgctatttc
    26341 ttctgccact tttgccgagc cttctttttc tgtgtcaaaa atttcggtcg gtattttttc
    26401 aagacgggtg accctcgaaa gttcaaagac atcatcgggt tgataatatc gtcgaggaat
    26461 acgcgtcatc gtaatttttg agcttaaatt tgttctcata agtaccttga taatgatgaa
    26521 tttggtttca aaagtaatac aatttcaatt aaaatgcaaa cgtttgcgtt aaattttgtt
    26581 tttttcttga ttttctttgg cagggtgaat tcttttgctt taatttgtgc agaaaattaa
    26641 attcaactta ccgaacaatg aatattatag ctgacagcgg atcgaccaaa acccactggt
    26701 gtgttataga aggtgaaaat accgtaaagg aattttacac aaaaggcatc aatccatttt
    26761 atcaaaccaa agaagaaatc cttgccgagc tgaaagaaaa tctttaccca cgaatcatgc
    26821 aaagtacgat tgatggagtt catttttacg gagcaggttg cgcctttgcc gaacaaaaaa
    26881 agatgttaag cgaaattttt agagatattt tgggaaaaga gcttgtcgta accatagaaa
    26941 gtgatttact cggagccgct cgctcattgt ttggaaagga aagcggaatt gcctgtattc
    27001 ttggaaccgg ctcaaattca tgttattaca acggcagagg aatagaaaaa aacgttccgc
    27061 cattgggatt tattctggga gatgaaggca gtggagccat tcttgggaaa aaactgacat
    27121 ctgatgcttt aaaaaatcaa ctaccgcccg atttggtgtc aaaattcatg aaagaatatg
    27181 atttgacatt ggaaaaggtt ctcgaccatg tgtacaaaaa gcctttcccg aatcgctatt
    27241 tggctcaatt tgtgcctttt ataaaggaaa atattcaata tccgccttta tataatattg
    27301 tatatagcag ctttaacgat ttttttaaca gaaatatttg ccaataccca catagtgaaa
    27361 aaacaccctt aggatttgtc gggtcagtgg cttttcactt tgctgacatt ttgcaaacca
    27421 ttgcacggga aagacatttc agaataattg atatagcgca aaatccaatg aagggattga
    27481 ttaaatatca ttcgcaaaaa aacaaataat tatttgaaaa tatggcattt gaaaaaatta
    27541 ccgaacaaaa ttcgctgcac aacgatttgg acaaaaaaga cattcgccaa ttgctcgttg
    27601 aaatgaatga agaagacaaa aaagtaccat ttgcagtaga aagagccatt ccacaaattg
    27661 agcaactgat tgaaaaactg gtagaacgaa tgaaaatagg tggacgttta ttctatatcg
    27721 gagcgggcac aagcggacgt ttaggtgtat tggacgcttc tgaaattcca cccacttttg
    27781 gcatgcctcc acatctggta ataggcgtga ttgcaggtgg tgatacggct ttacgcaatg
    27841 cggttgaatt tgctgaagac gatatggaac aaggttggga agatttgaaa aaacacaata
    27901 tttcttccat cgataccgta gttggcatca ccgcttccgg aacggcaccc tatgtgatgg
    27961 gagctttgag aaaagcaaaa caacatggaa ttttgacagc ttccattgcc tgcaatcccg
    28021 attcgcccgt atctcacgaa gccgacataa aaatagaact cattgtcggg ccggaatttg
    28081 taacgggcag tacacgtttg aaatcgggaa ctgcccaaaa gttgacactc aacatgattt
    28141 ccaccgttac catgataaaa ctcggaagag tgaaaggcaa taaaatggtc aacatgcaac
    28201 taaccaattc aaagttgatt gatcgcggca caagaattat tgtagaagaa ctgaatatcg
    28261 actacgaaca agcccgaaat attctgttga ttcatggctc agtgaaaaaa gccatagatg
    28321 cctacaaaat tcaaaataaa atttaacatt aaaattttgt taaatagaaa acaaacgttt
    28381 gcagtaaaag attttttttg tttatttgca atcacaaatg tttattaaaa acattatttc
    28441 cctaataaaa aaggctacat caaacaacgc aattcaaaca attaattatt ttagtaccta
    28501 tgaaaaaaat tcttctatta tttgctgttc tttttctttc ccttcaagtg tctttggcac
    28561 agaaggtaac agttacaggt acggtaacat ctaaggaaga tggacttcct atcattggag
    28621 cttcagttct ggaaaaagga acgacaaatg ggaccattac aaattataat ggggtttata
    28681 caatttcagt tcctatagga gctactttgg tatttagtta tgttgggatg gataaagtag
    28741 aacgcaaagt tactggagag ggcaaaatag atgtaataat gaatcccaca gcaattgcca
    28801 ttgatgaagt aatcgttact gctatgggtg tgaaagcaga gaaaaagaaa ttaaattttg
    28861 cagtacagtc ggtagatgct gaagctatta cagacggacg ttctcctaat tttgtcaatt
    28921 cattgcaagg aaaaatttca ggagttagtg taaccagttc cggaggttcc cccaatgccg
    28981 gttcacaaat tttattgaga ggaatttctt ccatcaaccc aagccaaaac aatgagcctt
    29041 tatttattct ggatgggatg gttctttccg gtggaggttc tgcagcagca gacattaatc
    29101 ccagtgatat tgaaaatgta accgttttaa aaggagcggc tgcttcagca ttatatggac
    29161 aaaatgcagc taatggagca ataatgatta caacaaaaac agcaaaagca gggaaaatgt
    29221 cgctttcagc caatgctagc atacaatcag atacgcctac gaggctactg gagttgcaat
    29281 caatttatgg accaggtgct ttgggattct atcgacctca ggaaagtgga ggatatggtg
    29341 gttggggtcc actattagac aaagatgaac ctgtttataa caatgtaaag aattattttc
    29401 aaagcggatt atatcaaaaa tacgatttca gtataaatgg tggaagtgat aaatttcagt
    29461 cttatgcatc tgtaaactat tcacgacatg atggtatagt accgaaagat tatcttgaca
    29521 aaatgggttt gctgttgaaa ggaaattacg aaatcaacaa taaaatttcc gccaatttca
    29581 tgaccaatat tgtaaagaac agttatcgag gcgcaggaag cattagtgcc atttattctt
    29641 ggccaatcaa tgatgatatt accaattata aaaattctga cggaagcatc cgatatcgat
    29701 atatcagtat tcccaaaaaa gaaaattcac ccattagtcc tttgtggagc aggtatatgg
    29761 attacggatt aaattcttct acccgtaatt tattacaggg ttcattaatt tacaagccac
    29821 tcaaaggcct ggaaattacg ggtcgattaa gtctcgatca aaacaattac atgtatgacg
    29881 gatatcaagt gccgcgtttt gacgattcca tcattctcga taatcctccg aaagaaagcg
    29941 attacaccaa tttggatgct tatgctcagg ctttgaaaga atacaacgac tattattatt
    30001 ctgtccctta ttttaatgaa aaagactatg cgagtgttga taaggacttt tttggcaatt
    30061 acagttattc acaaagcaaa agagaattgg ttactgccac tttgatggga aattacaaaa
    30121 ttcctcttcc aaaagatttt aatttagaag tacttgccgg tacagagatt aaaaccgata
    30181 atcgtatttc ttcttccatc aatggtagag attttgtaat accgggtaca tacagtatat
    30241 ccaatgtcag ggaaattgtg ggaacaaatg atgtgtcgtt gaaccattgg gaaaaaagaa
    30301 atgctggtgt tttcggtgaa ctgagaagtg attacaaagg attattaaat ttaagcgtta
    30361 cttcgcgctg ggactggtct tctaccattg catacaaata caatccttat ttttatccct
    30421 ctgtaacggg aggaataata ttttccgaaa ttttcggatt aagtaatgac tggttttctt
    30481 acggaaaatt acgcggcaat tgggcaagag tgggtaaaga tgtagccacg ccctatctgt
    30541 tcgaccgtaa atacactcaa tatccaacct tgccggatgg tggttatgga gccgatccta
    30601 ccatgtcggt agccagcaat ttgcaacctg aaatttccga ctcttgggaa attggcttgg
    30661 aaacgcgctt tttctccagc aaaaccagat tagatgtagc ttattattct acaactgtcg
    30721 acaatcagat cgtaacagtt cgtgtgtcgc cagcttcggg atatattttg caaaccagaa
    30781 atgaaggcaa tatcaaaaat cacggagttg aaatctcttt ggatcaggac attttgaaaa
    30841 ataaggactt attgtggact ggtaccttaa attttggctt taatcgcgga aaagttttaa
    30901 gccttcctga agacattgtg gaactgcagg gaacccaata cggagatatt tttcctacgg
    30961 cttacctgaa cggctctacc acagctattt caggaaaaga ctatttgcgt aacgaaaaag
    31021 gagaaataat ttgtacaccc gatggctatc ctaaaattaa tccaaccaaa agtgtgttga
    31081 ttggaaatcg cgaacctgat tttttgatgg gattgaccag tacactgaaa tacaaaggaa
    31141 tttctttaag ttttcttgtt gatggacgaa aaggagggga tgtgatgaat gtaacgggcc
    31201 gaagtctttg gtcgagtgga cagcacaaaa tgctggaatt ctatcgtgga cggcaggtag
    31261 tatgggatgg agtggtagaa caggctgatg gcacatacgt aaggaacacg actccaattg
    31321 ttctggacaa tcaaacaatc atcaattatt attacaatgt aagttcaaat ttcattgagg
    31381 atggttcata tattcggttg agttatgtaa cactgggata tgattttacc aacaagttaa
    31441 aaaacaatcg ctatattcac ggacttagat gttccttaac cggtaccaat ttatttcttt
    31501 taaccaaata ttccggttcc gatcctcaaa tcaatgcttc tactagcgaa ggaggaaccg
    31561 gctcgatggg aatagacaac tatcctgtcc ccaataccag aggaatcaac tttacaatta
    31621 atgccaactt ttaaatctca atacaacaat gaataccaca attatgaaag gaaacatttt
    31681 caaggttatc agccttatac tgctcgtttc catcggcagc ctgcttagca gttgcgaaga
    31741 ctggctggat attaacgtca gcaaagacaa accggtgaaa gttactcccg accaatcgtt
    31801 gccgctggtt ctgctgtatg catcccaact ttgctatgac catgccgaat acggagttta
    31861 tctatcacag gcacttacta ctgcaggacg cagtcaaaca gggtcgtacg cctacaaaag
    31921 tggatgggaa tttttatcga tgaaccgtca tcctcagtgg agaaggcatt tttacgatat
    31981 tggcgtcaac gccaaaggaa tgattgaaac tgctgaagaa atcggttcaa aaaactttgt
    32041 attgattggg cgtaccattc ttttgcattc aaccttgcta actaccgatg cttttggcga
    32101 tatgccacgt tcacaggttt acctgtccaa ttcaccttcg tacgacacac aagcttccat
    32161 atacgaatgg atgctgcagg aagcagatga actgataagc ctttataata atgacgaatg
    32221 ggtgaacaat ccaaacaaca agcgcattaa taaaaacatg gaccgcatgt ttgaaggcga
    32281 cttgggaaaa tggagggcat tcgcaaaggc cctaaaagca agaatactga tcagaaaact
    32341 tcccaactgg gaaaacaccc ctgaaacttg tcaaaaaatt atcgatgcag tagatgcagc
    32401 attaaatgat ccttcctata ccgatgccat ctacaagtat gatgggggtt cggccgaaaa
    32461 aaattgtccg tggggtccgg ctcaaccatc cttgaatttg ggttggccac agggacgaca
    32521 aaatctgctt tctgaagcga ttccttccaa attttttact tatgccatac ttggaagtta
    32581 ctcaagttac agggctacaa ctggttacgc tctcgacccc cgtgctgtgg agctgatgac
    32641 ccctcgttca aacgataaca acgtaaaagt tttacgctat ctcgaaaata acattggcat
    32701 gccggcaacc atgaaaatta ctttctaccc agatttatat gctactgaag gacataatcc
    32761 ctacacacgg aataacggtt acattatgct tatgtcgcgt gaagaactgc tgtttatcaa
    32821 agcagaagcc caatattgga aaggtgataa aactgctgcc tatcaaacaa caattgatgc
    32881 ggtaaatgtg aacatggaac gtttgggtgt attacctacc agtacggcta caaagcctta
    32941 ttacgatcgt ttcttttcta tcaagctacc cagtgccaat acctttacca ttgnnnnnnn
    33001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccttaca atctctacga agcctattgg
    33121 actacgccgg ataattacgg caccaatgcc aaattctcgc ccaatgcatg gattacccgc
    33181 ttgaattacg acccggaaac ggaagaaaaa tataaccgca aggaattaca aagacttggc
    33241 gcttataaga atccggattg gttgaaaaaa agaatgattt gggcatacaa gaacaacgac
    33301 aaggttactg ccgcagacca aacagagtgg aaataaattt aaactttaaa aattacgagt
    33361 atgaaaaaca tacaattaaa aatagctgtt ctgttgtcag tattcatggg cttttttctg
    33421 acttcctgtg aaaaaaatga cacgttaaac gataatgtat ttattggcga aatggctccg
    33481 caagtttatt gggaaatcaa tagcagcacg gtaaatgcag gcagtgaggt tccgtttttt
    33541 gtgcagtatt atacttcagg aaaagaaccg attgaccgcc ttgaagtatg gtacagcgta
    33601 gaagaagaag agtccaagac agtttcgtgt ccgtggactc aaaccttcac ttttcagctt
    33661 tcttcaacaa cttcggataa caagcgcata ttgcaaaaaa tctccgaata tcctcacaat
    33721 gaaagttact ggaacgattc tttgcatgct tattctttca atgccacttt tccaaccagt
    33781 tcaacattaa gttctatttc atggatcaaa cctgcaactt tcgacagcac caaaatgata
    33841 aaatattttg gtgaaggatt tatgcaacat ttcaaagaca gcctctacac aaaaatgaaa
    33901 gcactcgatt ttcaaaaaat gtattcaggg ctgaatttgg tagaaaactt caaagtgtat
    33961 ttggattcaa cttttaatga gaattccggc agttacgatt atcatttcaa gaaagattct
    34021 ctcgggaatg aagttattcc aaccgaaatt caaaatattt atcagactat tccttttgcc
    34081 gatttaattt ataatgcttc caacaacaat tacgatgttg aattttcacg cagttatcaa
    34141 ttgaatgcgc tcataaaagc gatcgacaga aaagggacag cgggattgtc aaccaaatcg
    34201 attataacac ttaattaacc aattgaaagc atggaatgta tctcatactt tatcaattca
    34261 atagcgggaa cattcataat tcgtgaaact attaaataat tcgcttatga aaacgaaaca
    34321 aatcataaaa tatctgggca tattcggttt gcttgtcttt tttaccggaa gttgcaagga
    34381 cgaaatgccg gcaattgaag aattcccttc accggatgta actttcactt attcggtgaa
    34441 tgataattcc tatcaattag attattatgt tggggcagaa ataaaatttg aaaccgcttc
    34501 tgctactact ggttgtacat gggattttgg agatggcaca aatgctaccg gcacaacagt
    34561 aatacataaa tatactcagg ccggaacata tcaggttaaa ctgactgtag aaggcaaagg
    34621 atacgtgatt caaaaaatat ttatcagcga tattcgtcct attatgaagt tgaaacccat
    34681 agaaggagga atgtgcgaag ttcaaactac ctatgtaagt tttgaagtgg aattacccaa
    34741 tcccgaaggt cttgaggaag aatacctttg gactttcccg gaaggaacgc ttgatgaaaa
    34801 tggaaattcc agagaaactt atacaggcaa agatccggga aaagtaaaat tcagcaatgt
    34861 tggttctcaa actgttcggt tgcaagtaaa attaggcggt cgccaactcg aagaaagcag
    34921 cattaatgtt caggttgcct tcaatagagc agttccaacg ctttattatg ccgtaaaaac
    34981 aggcaatatc atggcgctga aattaagttc ttctactccg gaagg
//
LOCUS       DAOTAX010000016        34986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1004_length_34986_cov_32.694770, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000016 DAOTAX010000000
VERSION     DAOTAX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 34986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34986
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1004_length_34986_cov_32.694770"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLM04_03170"
     CDS             <1..223
                     /locus_tag="PLM04_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02112.1"
                     /translation="AQAIKALQVKEAGEQKRAITHDLMGGWGSNSPTYSINSALNKIV
                     CKQQINNEKINNLPCEYSLPSVQKDRPYF"
     gene            complement(162..1826)
                     /gene="asnB"
                     /locus_tag="PLM04_03175"
     CDS             complement(162..1826)
                     /gene="asnB"
                     /locus_tag="PLM04_03175"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HON02113.1"
                     /translation="MCGIVCAFDIKKNSQELRPQVLQMSKRVRHRGPDWSGIFSNDRA
                     ILAHERLSIVDPESGKQPLYSKDGKLVLAVNGEIYNHQEIRKQFDGKYEFLTKSDCEV
                     ILALYREKGPDFLNDLNGIFAFALYDIEKDAFFIGRDHIGIVPLYQGWDKDGTYFVAS
                     ELKALEGYCNRIEEFLPGQYFYSLDGQPKQWYHRDWEDYENVKDNESNIEELRKALEA
                     AVERQLMSDVPYGVLLSGGLDSSVISAIAKKFSPKRIESGNTQDAWWPQLHSFAIGLE
                     GSPDLAAAREVAEHIGTIHHEIHFTVQEGLDALRDVIYHIETYDVTTVRASTPMYLLA
                     RFIKSMGVKMVLSGEGSDEIFGGYLYFHKAPNPEEFHKETVRKIKNLHLYDCLRANKS
                     LASWGVEGRVPFLDKEFLDVAMRLNPKDKMAGNGKIEKWILRKAFENYLPEKIVWRQK
                     EQFSDGVGYNWIDSLKEMTNKLVSDEEMEKAAEKFPIQPPMTKEEYYYRSIYSDLFPS
                     DSAALCVPSVPSIACSTPIALEWDASFKNMADPSGRSVKSIHKEGY"
     gene            complement(1865..3295)
                     /locus_tag="PLM04_03180"
     CDS             complement(1865..3295)
                     /locus_tag="PLM04_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HON02114.1"
                     /translation="MGNPKAFLYIPRKEAGYRPVQERIYDFGEVEQTLNREDRKLQAS
                     RCMDCGVPFCHWACPLGNKMPEWQDLIYKEKWQEAIDILQETNNFPEFTGRVCPAPCE
                     KSCVLSLHEAPLTIRENEAAITELAFNEGMIKARPPRKRIAKKIAVIGSGPSGLAAAQ
                     QLNRKGYTVTVFEKDAHIGGLLRYGIPNFKLAKNVIDRRLKLMEEEGIIFSPNMEVGK
                     NILAKELLDSFDAICIAVGAGQPRDIQAEGREMRGIYFAMDFLSQQNKQLLGETMQQE
                     EIISAKNKHVLVIGGGDTGSDCVGTSIRQGAVKVTQIEILPQPPIGKNPETPWPYYPL
                     ILKTSSSHEEGCTRRWCLNTKAFIGKNGHVTEVLIEEVEWHREENGRMTMVSTGKTET
                     VKADLVLLALGFLHPVHEGFLNELGVKYDGRGNIEVDKNGKTSVDKIFATGDSVMGAS
                     LVVRAIASGRKAAENIHHFFCQEQKK"
     gene            complement(3335..7849)
                     /gene="gltB"
                     /locus_tag="PLM04_03185"
     CDS             complement(3335..7849)
                     /gene="gltB"
                     /locus_tag="PLM04_03185"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HON02115.1"
                     /translation="MNYLNLYEQQSLYSFQNEHDACGVGLVVNISGEKSHEIIENGLQ
                     VLENMVHRGAESADNKTGDGAGMLLQIPHEFILLQGIPVPAIGKYGTGLIFLPREKDE
                     SELCLRIIKENVEEENLHLLAVRDMPVNSKILGEISASNEPLIKQIFVTGNLRQEELE
                     RKLYLVRKKIENSIAKSNLSNRRSFYIVSLSSKVLIYKGMLTSLQLRDYYPDLTNPNF
                     TSAIALVHSRFSTNTFPTWDLAQPFRMLAHNGEINTVRGNRLWMEARESVFKSELLGD
                     LKPLFPIVQPAMSDSASLDNVLEFLVMAGKSLPHALAMLVPESWNQKNPISEELRGFY
                     EYHSMFMEPWDGPATLLFSDGRFAGGMLDRNGLRPARYLITNDGMMIIASETGTIEFE
                     PENIKEKGRLKPGKILMVDTEEGKIYYDEELKEQIAKAFPYNEWLERNRINLEEISSG
                     RSVKPEIKNYQNLLIAFGYSKEDVDLLMLPMTAEGKEPTSSMGNDTPLAVFSEKPQRL
                     FNYFRQQFAQVTNPPIDPIREELVMSLTGYVGSIHKNLLEPVPDLCKMVKLKSPVLTN
                     TQFDILLNLRYKGFSTAVLPMLFNPKKGADGLVKAVDELCLLVEKAVDEGKNYIVLSD
                     RGVDEEHAPIPSLLAVSAVHHYLIDKRKRMQTDIIVESGEPREVMHFALLFGFGANAI
                     NPYMAFAVISERIKSDDIKLDFHTAESNYVKAVNKGLLKILSKMGISTLRSYRGAQIF
                     EAVGISSSVLNKYFKGVISKIEGIDMTDLANETLFPYWKAFYPEIDEENRLKNLGFYS
                     FRVEGEKHAWNPETIARLRIATRTGDYAKFKEYSSIIDNKPEAIFIRDLLDYKRNPID
                     VNEVEPVENIMKRFVTGAMSYGSISKEAHEALAMAMNKIGGRSNTGEGGEDPERYKQR
                     PDGLSTRSAIKQVASGRFGVTAEYLVNADEIQIKIAQGAKPGEGGQLPGYKVDNVIAK
                     TRHSIPGISLISPPPHHDIYSIEDLAQLIFDLKNINPAAMISVKLVSESGVGTIAAGV
                     AKAKADLILISGAEGGTGASPASSIKHAGLPQEIGLAEVQQTLVINNLRGQIRLQTDG
                     QLKTGRDIVISALLGAEEYGFATSALIVLGCVMMRKCHINTCPTGVATQNDELRKHFN
                     GRYEYLINFFTFLAQEVREHLAQMGYRSLDEIIGHTELLVRKEIKTSSKIEKVDLSKI
                     LFKPKQAEENAIRKTKNQEHKIDQVLDRILISEAMPAIEQCIPVKIRHKVRNTDRTIG
                     AMLSGEIAKRYGQVGLPEDTVQIQMEGSAGQSFGAFLSKGITLRLEGDANDYLGKGLS
                     GGKIIVVPPKESTFAPEKNIIAGNTLLYGATSGEVYINGIVGERFCVRNSGAIAVVEG
                     AGDHCCEYMTGGRTVVLGKTGRNFAAGMSGGIAYVLDEDGKFDMFCNMEMVELSLIEE
                     IADNKELHEFISKHFKYTGSPLAKRILDNWSEYSEKFLKVTPIEYKKVLQEEKIQAIN
                     KKIEQVERDY"
     gene            complement(7990..10188)
                     /locus_tag="PLM04_03190"
     CDS             complement(7990..10188)
                     /locus_tag="PLM04_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HON02116.1"
                     /translation="MSSLRFKAVEEAFKKKAAAVEVPSELTSEYFGKYVFTRSTMARF
                     LSEQTIKEIDEAIEKGTTLDRSIADKVAEGMKKWAMELGATHYTHWFQPLTDKTAEKH
                     DSFIDYLDDKPGENIIEKFSGKLLAQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAF
                     IVDDTLCIPTIFISYTGESLDYKAPLLKSLNSVDKAATAVCKLFDPKVKKVFSYLGWE
                     QEYFLVDENLYVTRPDLLLTGRTLLGHESAKNQQLEDHYFGSIPTRVVAYMKDLEFEA
                     YKYGIPLKTRHNEVAPNQFELAPIYGECNLAVDQNLLIMSIMKKVARRHGFRLLLHEK
                     PFAGINGSGKHNNWSLGTDTGVGLLTPGKTPEENLQFITFVVNILMAVYKHNALLKAS
                     IMSAQNAHRLGANEAPPAIISVFLGTQVSSVLDKLENSSSDEAIVVDSKKKLSLGIAH
                     IPEVLLDNTDRNRTSPFAFTGNRFEFRAVGSSANCSSAMTALNTAVAAQLIEFKAEVD
                     KKIASGTSKQTAIFEVLKEYIKISKPIRFDGNNYSDEWKEEAKLRGLDCETSVPKILK
                     AYTNEESIKIFTSTNVMSEKELHARNEVKWDMYTKKIQIESRVLGDLAINHIIPIATR
                     YQSILLDNVNKMKTVFSKEEAEKMCQLDLQLIKEIAQRVNTIKSDVDNMIEARKKANV
                     IECEEEKANAYHDTVLPFFDKIRYQVDKLEMIIDDEMWPLPKYRELLFIR"
     gene            complement(10867..12231)
                     /locus_tag="PLM04_03195"
     CDS             complement(10867..12231)
                     /locus_tag="PLM04_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HON02117.1"
                     /translation="MKKYIISLLALLVMGSIKISAATVVAEPKIDAGDTAWMITATAL
                     VLFMSIPALALFYGGLVRRKNVLNIFMQCMIIVAVISIEWIVCGYSMSFGKSEGFLSP
                     FIGGLDGAFLRNIGIHDLSPYFISHSQIAADGTLVGTIPHLVFVMFQCMFAVITPALI
                     IGAFAERINFKGFLLFTVLWSLFVYNPVAHWVWSSDGWLYKLGALDFAGGTVVHINAG
                     IAAIVTAIMLGKRRDYKGHALPPHNITYVVMGAAMLWIGWFGFNAGSGLAADGLAANA
                     FMVTHIAAAAAALTWAILDWFNDGKPTIVGTSTGAVAGLVAITPAAGFVNVGGALAIG
                     VLVSLICFAMVAFVKPKMGYDDSLDAFGVHGIGGITGSILTGLLATPTIQSAYSGAIA
                     GNWHQLLVQLIAVIAVLLYSGIVTVVLFKVSDKILGVRVPDKLEAIGLDETQHGETAY
                     TSFD"
     gene            complement(12288..12647)
                     /locus_tag="PLM04_03200"
     CDS             complement(12288..12647)
                     /locus_tag="PLM04_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HON02118.1"
                     /translation="MKKIEAVIRKSKFEDVKMALYEAGIEWFSYWDITGLGKSKEDQV
                     VRGQIFQSSYIQRRMLSIVVRDINLEKTVNAILDSAWTGDPGDGKIFVSDIEQTYRIR
                     TRESGPEALYNKDSNGI"
     gene            complement(12686..13336)
                     /locus_tag="PLM04_03205"
     CDS             complement(12686..13336)
                     /locus_tag="PLM04_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02119.1"
                     /translation="MKATKLLIICSLTISIPVITKSQNFTVSTDIVSSYVWRGVKYSD
                     ASIQPTLTFSHQNFSVGSWGSAGIDGFMEMDLFVNYAFPFGLSLGMTNYYFPPSKFFD
                     HSASTGSHAYEFNASYKISGFYAAGNYIFNEAGSAGSKGGDKYFEAGYDFKNIRLFAG
                     AGDGWHTPDGSFQAVNIGLSVTRDIQITDSFKLPLKGSAILNPATEQYYLTIGLSL"
     gene            13763..14494
                     /locus_tag="PLM04_03210"
     CDS             13763..14494
                     /locus_tag="PLM04_03210"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HON02120.1"
                     /translation="MSNINKISILFFGLLSQLFCIQLHADNNVKIIYNIKYGLVNGGK
                     AMVEKRDTVFGGKKCIYYRLEGNTTGLTDKIFKVHDTYESIVDAHTHLPYVAVRNAKE
                     KSYRYYNKVFFYHQKDSIFSERTGGMKVPPQLIDILSAFFYMVNVYDMDTLTEGYSLT
                     LHTINGHDIQKMKVKYVNNEIVQTDIGNSLCYVIAPEVKKGKLLKSSSGFKFYIAQNE
                     KIPYQIEIETKVGKIEAMPIEIIRK"
     gene            14596..15597
                     /locus_tag="PLM04_03215"
     CDS             14596..15597
                     /locus_tag="PLM04_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HON02121.1"
                     /translation="MVQTAYSPNPDRYQHSVGYRFCGKSGLKLPQISLGLWHNFGDVD
                     DAEEAEKMIQFAFDHGITHFDLANNYGTPAGSAEINFGKILKKNFASHRDEMVISTKA
                     GHEMWPGPYGDGGSRKYLISSLHQSLKRMNLDYVDIFYSHRYDPNTPIEETMGALSDM
                     VKQGKALYIGLSKYPEEKAREAFRILRENGTPCLIYQDRYNIFSRNIERSHFPLLKEN
                     GIGFIAFSPLAQGILTEKYLHGIPEHSRAALSYGFLQKSQITPEVLAKVQELNQMAHQ
                     RGQTMAQMALAWCLRTSDVNSVIIGASSVNQIQENIDALENISFTDDELWRIGELGA"
     gene            15640..16089
                     /locus_tag="PLM04_03220"
     CDS             15640..16089
                     /locus_tag="PLM04_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02122.1"
                     /translation="MKIFKAFIFFVLLTWVFIGCDYSSRSSIPDATVNFQLNMLVAPY
                     NTFNTPNSYLIFDKRINAIDRIGYGGILLYIDYEGKYCAFDLACPYEVNPNVKVTPND
                     EGKAVCEKCGSVFELLYGIGAPISGPAKEPLKRYHVYLSNNWLYVTN"
     gene            complement(16107..17207)
                     /locus_tag="PLM04_03225"
     CDS             complement(16107..17207)
                     /locus_tag="PLM04_03225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02123.1"
                     /translation="MNFILIGCFILVGSYLLMVIFFIIGWKRLSEFELSPHRMNGKFP
                     VSVVVSCHNEEEHLPRLLSALLRQTFSDFELVLVDDHSTDGTYKIMQEFSSAFSNMKF
                     FPSEIKGKKNALQKAVNIANGELMITTDADCEPCESWIETICNFYFQYRPDLIIAPVK
                     LKSNSKIFPQLQKFEFASLVASGAGAAAFGKSILCNGANLAFPKKIYSIHVNDLHNEL
                     LSGDDMFLLESVKKSKGEIRFLKSTLAMVETKTAENLRDFFRQRKRWSSKFSAYRDKD
                     VITVALLVFLTSFFLLCLFILSLITPRMWKYFLVCFMVKYLIDGYFLYSVRKFFDLRS
                     VWFYAFLLSLIYPFYVLITAVSGVFSGQKSWK"
     gene            complement(17531..18025)
                     /gene="tpx"
                     /locus_tag="PLM04_03230"
     CDS             complement(17531..18025)
                     /gene="tpx"
                     /locus_tag="PLM04_03230"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HON02124.1"
                     /translation="MATVKFAGTDVHTNGELPAVGSKAPDFTLVGKDLQEIHLKDFAG
                     KRVVLNIFPSLDTSTCATSVRKFNQWVSGKNNVVVICVSKDLPFAQSRFCGAEGLDNV
                     ITASDFRYNTFATDYGVLLVDGPLKGLMARAVVAIDENGKVIYNQLVDEISNEPNYDI
                     KVLD"
     gene            18125..19324
                     /locus_tag="PLM04_03235"
     CDS             18125..19324
                     /locus_tag="PLM04_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02125.1"
                     /translation="MKLSLLHKLGFFVSIVFLLLSCKDSHAYRVDSAFSTYLYTFQSE
                     AAKRGQVFNFQQEGLIMEFADLTDNKAGLCHYEKPIRIEIDRTYWNDISKYAGAEYMK
                     EDVIFHELGHGFLGREHLNDVLENGDWKSMMCGGQKVNNRAWNINYRGIRRAYYLDEL
                     FNQNTPSPVFSTENFSIDTSAYTLKVRKSFDTALDAGWKIENTSEYETSIDNRRLKFE
                     SKVDPVYFVYAKTNVDVQSDFIYQLQIECKSKNDSDQYGLIFGSVPDQGASYVQKSLE
                     YFTINNLQKMYMGNTTWYSFYTELTKPCIIPSGNNRMKIVKSGEMLYYFINDVYCYCS
                     EMEVKDSGFYFGFIVPPKSTVWLDNLIVSQQITSQSAVSKVRELPVFDAQVVSEPKFR
                     MKCIKGK"
     gene            19373..21055
                     /locus_tag="PLM04_03240"
     CDS             19373..21055
                     /locus_tag="PLM04_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HON02126.1"
                     /translation="MSKFVIYQVLPRLFGNYNSNNKYNGSIEENGCGKFNSFTTKALK
                     EIKDLGVTHIWYTGIFEHATQTDYSAYGIRKDFPCLVKGKAGSPYAVKDYYDVDPDLA
                     ENVPDRMKEFEDLIHRTHKEGMKVLIDFVPNHVARQYFSDAKPENVVDLGEHDHPEWA
                     FSPLNNFYYLPGQPFRPNFDIDGYNEFPAKVTGNDQFTPSPTLNDWYDTVKLNYGINY
                     MEGMQKQFDPIPDTWNKMLDILLFWASKKVDGFRCDMAEMVPVEFWNYAISRVKEKYP
                     KIIFIAEIYKPAEYRNYLANGKFDYLYDKVGMYETLRNVTSRNYPARNITYAWQQLNG
                     IEDHMLNFMENHDEHRIASGFFAGDGSYAQPAMIVAATLTKAPVMIYFGQELGELGMD
                     NEGYSGLDGKTTIFDYWGVKSVQAWANNGKFDGENLTAEQKELRQFYQKLLNITLQEK
                     AITEGVMYDLEYANFNNPHFDTHEQFAYIRKFKDEMLLFVLNFDDKELYTEVRIPLEA
                     FQYLELMPGCRYKAVNLLDEVETIEQITLIPDMIFKISIPAWKGKIFKFKKL"
     gene            21165..21635
                     /locus_tag="PLM04_03245"
     CDS             21165..21635
                     /locus_tag="PLM04_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HON02127.1"
                     /translation="MKAYFGLFAGMALLLISCSGVKTTPESVKSLTQKVENKNFTVKV
                     QYAQPLSGKQIFLTSEYVLTVKNDSAEAFLPYFGVAYSAPYGGDGGIRFNERMTDFSI
                     MPNKRNNGWDIHFKVKAPEDYYEFFLKIFNDGSANFTVNSNRRQSISFMGEVKN"
     gene            22217..23713
                     /gene="zwf"
                     /locus_tag="PLM04_03250"
     CDS             22217..23713
                     /gene="zwf"
                     /locus_tag="PLM04_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HON02128.1"
                     /translation="MLVIFGSNGDLARRKLLPSIFQLHADRLLPESFAVIGTGSQEKE
                     QSVFLQEVEESLKKYVKSDSVNDDQTVREFLKKVYYLPVRNQVDEDFGKLKEYLHSLS
                     AKLNIPRNIIYYFSIPPFLYEVVASNLVKYGLNRQKDGWKRIIVEKPFGYSYETALDL
                     EKKLHAGFQEDQIYRIDHYLGKETVQNIMVTRFANGFFEPIWNSKYVDRVEITAAEKI
                     GVGNRGGYYDTAGALRDMVQNHLLQVMAIVAMEPPAVFDSDSIRNETVKVLQALRPID
                     EEHVAENVVRGQYIETMVDDVLQKGYRQEKGVASNSRTETYVAMKLFIDNWRWADVPF
                     YIRTGKQLPVQVTEVVIHLKPAPHQLFKQRCITQSTNMIILRLNPDAGIAIDFGMKIP
                     GAGYKVQNVNMAFHYSDLAHSKIPEAYERLILDCMMGDSTLYARADALRASWKFVDPI
                     LNAWKNNPEIPLYFYECNTWGPNEANALFGDKRLKWRPPFNKFKTTEE"
     gene            23749..24501
                     /gene="pgl"
                     /locus_tag="PLM04_03255"
     CDS             23749..24501
                     /gene="pgl"
                     /locus_tag="PLM04_03255"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HON02129.1"
                     /translation="MKQYDIHVYNTPEDTAKAIAQLISNRINEKQNSLSSFNLAISGG
                     KTPQTLFNILANDFAEKIPWHQLRLFWVDERCVPPTHPESNFGMTNETLLKKVPLPKK
                     NVFRMKGETVPEEEAIHYQSLLTKELPSKNGIPVFDLILLGMGNDGHTASIFPKDINL
                     IDSHLSVAVTQHPDTGQKRITLTGPVLKQADEIIFFVTGASKATILKEIVLNEPPAKK
                     YPAFHIYSVEGKTSFYLDKEAAALLPKELPLL"
     gene            complement(24595..25860)
                     /locus_tag="PLM04_03260"
     CDS             complement(24595..25860)
                     /locus_tag="PLM04_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HON02130.1"
                     /translation="MFGLVDCNNFYVSCERVFNPSLIGRPVVVLSNNDGCVISRSNEA
                     KALGIPMGQPAYQLKELIEKQNVAVFSSNYVLYGDMSHRVMTTLASFVPDVEVYSIDE
                     AFLLLDGFEKQNLKELGRKIVETTVRNTGIPVSMGIAPTKTLAKVANKFAKKFIDYKG
                     LCLIDTDEKREKALQLTAIEDVWGIGRQYSKMLAYQGIRTAWDFTQKSKAWVRRNMSV
                     TGERTWLELQGNPCIEMEHVAAKKSICTSRSFGERLTEYAPVSEAVANFAANCAEKLR
                     KQHSKAMLLTVFIHTNPFSTSQLQYFNQKTVPLPVPTNDSAELIHHALQALKAIFAEG
                     YLYKKAGVVVGDIVPETPLQTDLFDTRDREKFGKAMQVMDSLNATYGKHKVRIAAQGF
                     GRNWKLKNEKLSPFYTTRLKDILEVKATE"
     gene            complement(25913..26386)
                     /gene="umuD"
                     /locus_tag="PLM04_03265"
     CDS             complement(25913..26386)
                     /gene="umuD"
                     /locus_tag="PLM04_03265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HON02131.1"
                     /translation="MKADKIATSGYIDFYSVNTESAFYLPIADGGIAAGFPSPAQDYI
                     DVKIDLNRELIANPSSTFLGRVKGTSMKDAGIEDGDILIIDKSIEPQDGDMAVCFLDG
                     EFTLKYIHMEKDAVYLMPANPKFKPIKVTEENHFCIWGIVTYSIKHHKQKQGKTK"
     gene            complement(27034..27106)
                     /locus_tag="PLM04_03270"
     tRNA            complement(27034..27106)
                     /locus_tag="PLM04_03270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27071..27073),aa:Gly,seq:ccc)
     gene            complement(27222..27797)
                     /gene="rsxA"
                     /locus_tag="PLM04_03275"
     CDS             complement(27222..27797)
                     /gene="rsxA"
                     /locus_tag="PLM04_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HON02132.1"
                     /translation="MTYILIFISAVFVNNIVLSQFLGICPFLGVSKKINTALGMSGAV
                     VFVMTIATIFTYIIQKSILEAYNIAFLQTITFILVIAALVQLVEIILKKISPALYQAL
                     GVFLPLITTNCAILGVAILVVQKNFNLLESVVFAISTSIGFGLALILFSGIREQLSMI
                     KVPKKMEGAPIALLTAGLLALAFMGFSGIVK"
     gene            complement(27861..28451)
                     /locus_tag="PLM04_03280"
     CDS             complement(27861..28451)
                     /locus_tag="PLM04_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HON02133.1"
                     /translation="MSNSKVKIILNGLITENPIFVLILGMCPTLATTSSAINGFSMGL
                     ATSFVLIGSNVAISLLKNLIPDKVRIPAYVVVIATFVTVVEMLMQAYLPALFESLGIF
                     IPLIVVNCIVLGRAEAFASKNSVIDSSLDGIGIGLGFTFALTLLGIVRELLGTGKIFS
                     LSVLPEKYGMLVFVLAPGAFIALAYLIAIMNKIRKA"
     gene            complement(28507..29133)
                     /locus_tag="PLM04_03285"
     CDS             complement(28507..29133)
                     /locus_tag="PLM04_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HON02134.1"
                     /translation="MAKLTSSFKNMVISLLSITVFSALTLGSVYTLTKEPIEKSKTAK
                     LQKALKEVLPTFEHLDTAIKVPVNNVDTLNVYKAYDKNNVFVGAAVEANSKNGFGGEI
                     KIMIGFDKEGNIINYSVLEQKETPGLGTKMVDWFKMSNTKHDIRGKNPAKHRLLVSKD
                     GGEIDAITAATISSRAFLEAVNKAYQAYANNPKAPKTHDVSSGASNIN"
     gene            complement(29126..30094)
                     /locus_tag="PLM04_03290"
     CDS             complement(29126..30094)
                     /locus_tag="PLM04_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HON02135.1"
                     /translation="MNKLIVSPAPHVHSGNSIRKEMVSVLIALIPAYLVALYYFGVGA
                     IIVSVTSVISCVIFEYLIQKFLIKGENTVSDYSAVLTGLLLSFNLPSNLPVWIVVIGA
                     LVAIGVGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTSWPRPMAWNTAYLDAETSAT
                     ALSALKEHLGNIPSLFDMFVGNMGGSMGEVSALALLLGFVYLLFKRTITWHIPVSILL
                     TVFIMTGIFYLVNPQTYVSPIIHLLSGGLMLGAIFMATDFVTSPMTKMGMTIYGIGIG
                     IITVVIRMFGSYPEGVSFAILIMNAFTPLINTYIKPKRFGEVRKNG"
     gene            complement(30113..31450)
                     /gene="rsxC"
                     /locus_tag="PLM04_03295"
     CDS             complement(30113..31450)
                     /gene="rsxC"
                     /locus_tag="PLM04_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HON02136.1"
                     /translation="MKTFKMGGVHPPENKFSAGKKTKIISLPQQVIIPLRHYSGNPSV
                     AEVSRGEIVKVGQLIGRANGFISANVHSSVSGKVSKIDNAMDAFGYKFPAVFIDVIGD
                     DWLDEIDLSPEVKRVCNLEPHEIIKKVAEAGIVGLGGATFPTHVKLSIPQGKKAEVLI
                     INGAECEPYLTCDHQLMLEKGEEIIIGISILMKAIRVKKAIIGIESNKMDAVLNLEKW
                     AKTMEGIEVVPLKVKYPQGSEKQLIQALTGREVPSGALPIEVGAVVQNVATTFAVYEA
                     VQKNKPLIERIITVTGKSVPEPGNYWVRIGTPFTHIIEETGGMPQDTGKVVAGGPMMG
                     RALPSLDLSVAKGTSSILFIPSSESKRGKVFNCIRCAKCVFHCPMGLEPYLLMNQGEK
                     QMWENMEENRVMDCIECGSCSFICPSNRPLVDYIRYGKSTVGTMIRNRKTQKS"
     gene            complement(31510..32373)
                     /locus_tag="PLM04_03300"
     CDS             complement(31510..32373)
                     /locus_tag="PLM04_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HON02137.1"
                     /translation="MNLILASVVSLGGIGALSAVILYFIAQKFHVEEDARIDEVEAVL
                     PAANCGGCGFPGCRAFAAACVTADTLDNLSCPVGGTATMEKVADILGKKAAAVVPTIA
                     VVRCGGTCEVREKTNSYDGVKSCTIVHNLYGGETGCSWGCLGLGDCEVSCTFDAIHIN
                     PETMLPEVDEDKCTACGACVKACPKNIIELRRKGPKSRRIYVNCVNKDKGAVNKKVCK
                     VACIGCSKCFNVCPYEAIEMGNNLAYIDSEKCKLCRKCVEVCPTNAIVELNFPPRKTK
                     IEEPSTTGTEK"
     gene            complement(32413..32832)
                     /locus_tag="PLM04_03305"
     CDS             complement(32413..32832)
                     /locus_tag="PLM04_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HON02138.1"
                     /translation="MQQIEHIGIVQHIEGSKAQILIIQQSACSACHAKSACTVMEEEK
                     KIVDVVVTDSSIKEGDQVVIFGRNSMGLQAVLLAFIFPLLLIVLTLIISNYFQQNETI
                     SGIIALCSIFPYYGILSLFNHKLKRKFEFHVKKESLT"
     gene            complement(33830..34213)
                     /locus_tag="PLM04_03310"
     CDS             complement(33830..34213)
                     /locus_tag="PLM04_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HON02139.1"
                     /translation="MTENEISYKIRGAIFAVYNNVGPGLLESAYEAALSYELKNAGLE
                     VKTQVGLPFVYEDIALEIGYRVDILVENKVIIETKSVEQLTDVHYKQLLTYLKLSEKK
                     LGILVNFNTDNIEASIKRIVNHLEE"
     gene            complement(34800..>34986)
                     /locus_tag="PLM04_03315"
     CDS             complement(34800..>34986)
                     /locus_tag="PLM04_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HON02140.1"
                     /translation="NIETRPLWKPMHLQPVFACCPYYGDNTEGRLFDDGLCLPSGPIL
                     TDEDIQRVVDVIKKMYY"
BASE COUNT        10808 a         7069 c         6288 g        10821 t
ORIGIN      
        1 agctcaggcg ataaaggcgt tgcaagtaaa agaagcaggc gaacagaaac gagccataac
       61 gcacgatttg atgggagggt gggggagtaa ttcccctacc tactcgatta actctgcatt
      121 aaataagatt gtttgcaagc agcagatcaa caacgaaaaa atcaataacc ttccttgtga
      181 atactcttta ccgagcgtcc agaaggatcg gccatatttt tgaatgaagc atcccactcg
      241 agagcaatgg gtgtgctgca tgcaatagag ggaaccgaag gcacacaaag cgctgctgaa
      301 tcggacggga acaaatccga gtaaatggaa cggtaataat attcttcctt agtcatggga
      361 ggctgaatgg gaaatttttc ggctgccttt tccatttctt cgtcgcttac caacttgttg
      421 gtcatttctt ttaatgaatc aatccagttg taacccacac catccgaaaa ctgttccttt
      481 tgccgccata cgattttctc gggcaaataa ttctcaaatg ctttacgcaa aatccatttt
      541 tctatttttc cgtttccggc cattttatct tttggattta accgcatggc tacatccaaa
      601 aattctttgt ccaaaaaagg aacacgacct tctacacccc acgacgccaa cgatttattg
      661 gctctcaaac aatcgtacaa atgcagattc ttgatttttc tgacggtttc cttgtgaaat
      721 tcttcgggat ttggagcttt gtgaaaataa agataacctc caaaaatttc atctgaacct
      781 tcaccggaaa gaaccatttt aacgcccatc gatttaatga aacgtgccag caaatacatg
      841 ggtgtactgg ctctcacggt agttacgtca taagtttcga tatgataaat aacatctcgt
      901 aaagcatcca atccttcctg aacggtaaaa tgaatttcgt gatgaatggt gccaatatgt
      961 tcggccactt cacgtgctgc agccaaatcg ggcgaacctt caagtccaat ggcaaacgaa
     1021 tgcagctgcg gccaccacgc atcctgagta tttccacttt cgatacgctt gggcgaaaat
     1081 ttttttgcta ttgccgaaat tacggaagaa tctaaacctc ccgaaagcaa tacgccgtag
     1141 ggaacatcgg acataagctg acgttcgacg gctgcttcaa gtgctttgcg caattcttca
     1201 atattgcttt cgttgtcttt cacattttca taatcttccc aatcacgatg gtaccattgt
     1261 ttgggttgcc cgtccaaact gtaaaagtac tgtccaggaa gaaattcttc gatacggttg
     1321 caataacctt ccaaagcttt caactccgaa gcaacaaaat aagttccatc tttgtcccaa
     1381 ccctgataaa gtggaacaat accaatgtga tcacgtccga taaagaaagc atctttttca
     1441 atatcgtaga gtgcaaaagc aaatattcca ttcaaatcat tcaagaaatc ggggcctttt
     1501 tcccgataaa gcgccaaaat tacttcacaa tccgatttgg taagaaattc gtattttcca
     1561 tcgaactgtt tccgtatttc ctgatgatta taaatttcgc catttacggc aagtaccaat
     1621 tttccatctt tactgtacaa gggctgcttg ccggattcgg gatcaacaat cgacaggcgc
     1681 tcatgcgcca aaattgccct gtcattgcta aaaatgcccg accagtcagg tccgcgatgt
     1741 cttactcttt tcgacatctg caaaacttga ggacgcagtt cttgggaatt cttttttata
     1801 tcaaaagcgc aaactattcc gcacatattt ctctaatttt ttatgaaaaa acattttctt
     1861 tgattcattt tttctgctcc tggcagaaaa aatggtgtat attttcggct gcttttcgtc
     1921 ctgaagctat ggctctaact accaaacttg ctcccattac cgaatcaccg gtagcaaaaa
     1981 ttttatccac cgaagttttc ccgtttttgt caacttcaat atttcctctg ccatcatatt
     2041 ttacacccaa ttcgttaaga aagccttcgt gtaccggatg cagaaatccc aatgccagta
     2101 aaaccaaatc agccttaacg gtttccgttt tccccgtaga aaccattgtc attcgtccgt
     2161 tttcttctct gtgccattct acttcttcaa ttaagacttc cgtaacgtgt ccatttttcc
     2221 cgataaaagc ttttgtattc aggcaccaac gccttgtaca gccttcttcg tgtgaactgg
     2281 aagtttttaa aatcaacgga taataaggcc agggcgtttc cggatttttt ccaatcggtg
     2341 gttgtggtaa aatttctatt tgcgtaactt ttacggcacc ttgtctgatg gatgttccaa
     2401 cacagtctga gccggtatcg cctccaccaa ttaccaacac atgtttgttt ttggccgaaa
     2461 taatttcttc ctgttgcatt gtttctccaa gcaactgttt gttttgctga ctcagaaaat
     2521 ccattgcaaa ataaattcct ctcatttctc ttccttcggc ctgaatatca cgtggttgtc
     2581 ctgcaccaac agctatgcag attgcatcaa aactgtccaa cagttcctta gctaaaatat
     2641 ttttcccaac ttccatattg ggactaaata tgattccttc ctcttccatc aacttcaatc
     2701 gcctatcaat tacatttttt gccaacttaa aattaggaat gccataacgc aacaagcctc
     2761 caatatgagc atctttttca aaaaccgtaa ccgtgtatcc ctttcggttc aattgctggg
     2821 cagcagccaa accagacgga ccagaaccaa taacggcaat ttttttcgct atccgttttc
     2881 ttggaggacg ggctttaatc atgccttcat taaaagctaa ttcggtaata gcagcttcat
     2941 tttcacgaat cgttaaaggt gcttcatgca aagacagcac acacgatttt tcacaaggag
     3001 ccggacaaac tcttcctgta aattcaggga aattattggt ttcctgcaaa atatcaatgg
     3061 cttcctgcca tttctctttg taaatcaaat cctgccattc gggcattttg ttcccgagcg
     3121 gacaagccca atgacaaaac ggaactccac aatccatgca tctcgaagct tgaagtttcc
     3181 tgtcttcacg attcagggtt tgttccactt ctccaaaatc gtaaattctt tcctgaacgg
     3241 gccgataacc ggcctcctta cgcggaatat ataaaaatgc tttcgggttt cccataattt
     3301 tgaaatttaa acttttattg aacaacacgt tacatcaata atctctttca acctgctcga
     3361 tttttttatt gattgcctga attttttctt cctgcaacac tttcttgtac tcaatgggag
     3421 taactttcag gaatttttcg gaatattccg accaattatc caaaatacgc tttgccaaag
     3481 ggcttcctgt gtatttgaaa tgtttggaaa taaactcgtg aagttccttg ttatcagcta
     3541 tttcttcgat aagagaaagt tccaccattt ccatgttgca aaacatatca aatttgccgt
     3601 cttcatccag cacatatgct attcctccgc tcatccctgc ggcaaagttt ctacctgttt
     3661 ttcccaacac aacggttctt cctcccgtca tgtattcgca gcaatgatca ccggcacctt
     3721 ctacgacagc aattgctccg gaatttctta cacagaaacg ttcgcctaca ataccattga
     3781 tatacacttc acccgaagta gctccataaa ggagtgtgtt gccggcaata atattttttt
     3841 cgggagcaaa agtactttcc ttcggaggaa caacaatgat tttcccgccc gaaagacctt
     3901 tcccgagata atcgtttgca tcgccttcga gacgcagcgt aatgccttta gaaagaaaag
     3961 cgccaaaact ttgtcctgca gaaccttcca tctgaatttg aacggtatct tccggcagtc
     4021 caacctgtcc ataacgtttg gctatttctc cggaaagcat agctcctatg gttcgatcgg
     4081 tatttctgac tttatgtcga attttaacag gaatacattg ttcaatggcc ggcatggctt
     4141 cggatattaa tattctgtcg agcacctgat caattttgtg ttcctgattc tttgtttttc
     4201 tgatggcatt ttcttctgcc tgcttcggtt taaaaagaat tttggacaaa tcaacctttt
     4261 caatttttga actcgtttta atttctttgc gtaccaacaa ttcggtatgt ccgataattt
     4321 catccagact tctatagccc atttgagcca aatgttctct cacttcctgt gccaaaaagg
     4381 taaagaaatt tatcagatat tcgtatcttc cgttgaaatg tttgcgaagt tcgtcgtttt
     4441 gtgtggccac acctgtagga caggtattta tatgacattt tctcatcatg acacatccca
     4501 aaacaatgag ggcgctggta gcaaaaccat attcttcagc tcccaacaaa gcagaaatca
     4561 caatatcccg tcccgttttt aactgaccat ccgtttgcag tcggatttgt ccacgcaaat
     4621 tattgatcac caacgtttgt tgaacttctg cgagaccaat ttcctgtgga agtccggcat
     4681 gtttaatgga actggcggga cttgctccgg taccaccttc tgctccgctg ataagaatca
     4741 aatcggcttt tgcttttgcc actccggcag caatagtacc cacaccactt tccgaaacaa
     4801 gttttacgct aatcatggca gccggattga tattttttaa gtcaaaaatc aattgggcaa
     4861 gatcctcaat agaataaatg tcgtgatggg gcggcggaga aattaaagaa attccgggta
     4921 tggaatgtcg ggtttttgca ataacattat cgactttgta tccgggaagt tgtccgcctt
     4981 caccgggttt tgcaccttga gctattttaa tttgaatttc gtcggcattt accaaatatt
     5041 cggccgtaac tccgaatctg ccggaagcaa cctgcttgat ggcgctgcgc gtacttaaac
     5101 cgtcaggtcg ctgtttataa cgttcaggat cttcgcctcc ttcaccggta ttgcttctac
     5161 ctccgatttt attcattgcc attgccaatg cttcatgcgc ttccttgcta atagaaccat
     5221 aggacatggc tcctgttaca aatcgcttca taatgttttc taccggctca acttcattta
     5281 catcaatggg attgcgcttg taatcgagta aatcgcgaat aaaaatggct tccggtttgt
     5341 tgtctataat gcttgaatac tctttgaatt ttgcataatc tccggttcgc gtagcaatgc
     5401 gcaaacgggc aatggtttcc ggattccatg catgtttttc tccttctacg cgaaatgaat
     5461 aaaaaccaag attcttcaaa cgattttctt catcaatttc aggataaaaa gctttccaat
     5521 aaggaaataa agtctcattg gccaaatcgg tcatatctat tccttcgatt ttggaaatga
     5581 ctcccttgaa atatttgttc agcacggatg atgaaatacc tacagcttca aatatctgag
     5641 cgccgcgata acttcttaag gtagaaattc ccattttcga aaggattttc aaaagcccct
     5701 tgttcacagc tttaacataa ttcgattcgg ccgtatggaa atcgagtttg atatcgtccg
     5761 atttgatgcg ctcgctgata acggcaaaag ccatataagg attgattgca ttggctccaa
     5821 aaccaaatag caaggcaaaa tgcatcactt cacgcggttc tccactttcg acaataatat
     5881 cggtttgcat tcgtttccgc ttgtcgatga gataatgatg taccgcagaa accgcaagca
     5941 gcgaaggaat gggagcatgt tcctcatcaa cacctctgtc gctcaacaca atataatttt
     6001 ttccttcatc caccgctttt tctaccaaaa ggcaaagttc atctactgcc ttcaccaaac
     6061 catcagctcc ttttttcgga ttaaaaagca ttggtaacac tgcagtagaa aaacctttgt
     6121 aacgtaaatt aagcaaaata tcgaactgag tattggtcag aaccggactt ttcagcttta
     6181 ccattttaca aagatccgga acaggttcga gcaaattttt atgaatagag ccaacgtaac
     6241 cggttaaaga catgaccaat tcttcacgaa tcggatcaat gggtggattg gttacttgag
     6301 cgaattgctg acggaaataa ttgaataacc gttggggttt ctcggaaaaa acagccaaag
     6361 gggtatcatt tcccatcgat gaagtaggtt ctttcccttc agcggtcata gggagcatca
     6421 gcaaatcgac atcttccttt gaataaccga aagcgataag cagattctga taatttttta
     6481 tttccggctt tacacttctg cccgacgaaa tttcctccag attgattctg ttcctttcca
     6541 accattcgtt gtaaggaaat gctttggcaa tttgttcctt taactcttcg tcataataaa
     6601 ttttgccttc ttcagtatcc accatcagaa ttttaccggg tttcaatcgt cccttttctt
     6661 taatattttc cggttcaaat tctatggtgc cggtttcgga ggcaataatc atcataccat
     6721 cattggtaat aagatagcgg gccggtcgca gaccgtttcg gtcgagcatg ccaccggcga
     6781 agcgtccatc actgaaaaga agcgttgccg ggccatccca aggttccata aacatgctat
     6841 ggtattcata aaaacctctc agttcttcgg aaataggatt cttttgattc caactttctg
     6901 gtaccaacat ggccaatgcg tgaggcagac tttttcctgc cataacgaga aattctaaaa
     6961 cattgtccag agaagcactg tcgctcattg caggctgaac gatgggaaac aaaggtttta
     7021 aatcgcccag caactccgat ttgaatacac tttcacgcgc ttccatccat aatcggttgc
     7081 ctctcactgt attaatttca ccgttatgag ccaacatcct gaaaggttga gccaaatccc
     7141 aggtcgggaa agtgttggta ctgaaacgtg aatgaaccaa agcaatggca ctggtaaaat
     7201 tgggattggt caaatcggga taatagtcac gcaattgaag tgatgtgagc attcctttat
     7261 aaatcagtac tttggaagaa agacttacta tataaaagct ccttctgttg gaaagattgg
     7321 attttgctat cgaattttca atttttttcc ttaccagata gagttttctt tccaactctt
     7381 cctgacgaag attaccggta acaaatattt gctttatcag cggttcgttg gatgcagaaa
     7441 tttcacccaa aattttgctg ttgaccggca tatcacggac tgccaacaaa tgaagatttt
     7501 cttcttcaac attttcctta ataatacgta aacaaagttc tgattcatct ttttcgcgtg
     7561 gcagaaatat aagaccggtg ccatattttc cgatagcggg gactggaatg ccttgtagca
     7621 gaataaattc gtgtggaatt tgcagcagca taccggcacc gtcgccggtc ttgttatcgg
     7681 cactttctgc tccgcgatga accatattct cgagcacttg taatccattt tcaatgattt
     7741 cgtgggattt ttcaccggaa atatttacca ctaaaccgac tccgcatgca tcatgctcat
     7801 tctgaaaaga atacaatgat tgctgctcgt ataaatttaa ataattcata atcaattttt
     7861 taattttata gagatgggtt caaaatttgt caactgcaaa ataacagttg acaaattttg
     7921 aaggtagaaa aaactgtgtg caataaaaaa cctaagaagc ttattaataa aggatttttc
     7981 gggtaagact tagcgaatga aaagcaattc tctatatttt ggtaaaggcc acatctcgtc
     8041 atcaataatc atttcgagtt tgtccacctg ataacgaatt ttgtcgaaaa atggtaagac
     8101 ggtatcgtga taagcattgg ccttttcttc ctcacattca attacattgg ctttttttct
     8161 ggcctcaatc atattatcta catccgactt gatagtgttc actctttggg caatttcctt
     8221 tatcaactgc aaatcgagct ggcacatttt ctcggcttct tcctttgaaa ataccgtttt
     8281 cattttgttg acattatcca gcaaaataga ttgataacgt gtagcaatcg gaattatgtg
     8341 attgatggcc aagtcgccca atacacggga ttcaatctga attttcttgg tgtacatatc
     8401 ccatttaact tcatttcgtg catgaagttc cttttcggac atgacattag tagaagtgaa
     8461 aattttgata ctctcttcgt tggtataagc tttcaaaatt tttggcacac tcgtttcaca
     8521 atccaatccg cgaagttttg cttcttcctt ccattcgtcg ctatagttgt tgccatcaaa
     8581 acggatgggt tttgatattt ttatgtattc tttcaacact tcaaaaattg cagtttgctt
     8641 gctggttccg gaagcaatct ttttatccac ttcagcttta aattcaatta attgtgcagc
     8701 tacagctgta tttaaggctg tcattgccga agagcaattg gctgaagaac ctacggcgcg
     8761 gaactcaaac ctgttgcctg taaatgcaaa aggcgaagtt ctgtttctgt cagtattgtc
     8821 gagtaacact tcgggaatat gggcaatacc caaactcagt tttttcttgc tgtccaccac
     8881 aatggcttca tcgctgctgc tattctcaag tttatccagc acactgctca cctgtgtacc
     8941 aagaaaaaca gaaataatag ccggtggtgc ttcgttggct cccaagcgat gggcattttg
     9001 agccgacatg atggaagctt tcaacaacgc attgtgtttg taaacggcca tcaaaatatt
     9061 tactacaaaa gtgataaatt gaagattttc ttcaggagtt ttaccaggag taagcaagcc
     9121 aactcccgtg tcggttccca gcgaccaatt gttgtgtttt cccgaaccgt ttattccggc
     9181 aaaaggcttt tcgtgaagca acaaacggaa gccgtgtcga cgagcaactt ttttcataat
     9241 ggacataatt aacaaattct gatccactgc caaattgcat tctccgtaaa tgggtgccaa
     9301 ttcaaactga ttgggagcta cttcgttatg tctggttttc aagggtatgc catatttgta
     9361 ggcttcaaat tccaaatctt tcatataagc taccacccga gtaggaattg aaccaaaata
     9421 atggtcttcc aactgctgat tttttgccga ctcgtgtccc agcaaggttc gaccggttag
     9481 caataaatcg ggacgggtaa catacagatt ttcatctacc agaaaatatt cctgttccca
     9541 acccagataa gaaaaaactt ttttcacttt tggatcaaac aatttacaaa ccgctgtagc
     9601 tgccttatcc accgagttga gcgattttaa caaaggagct ttgtaatcga gcgattcgcc
     9661 ggtgtaagaa atgaaaatgg tagggataca caacgtatcg tcaactataa acgctggtga
     9721 tgaaggatcc cacgccgtat aaccacgcgc ttcaaaagtg cttctgattc ctccgctcgg
     9781 aaaagacgaa gcatcgggtt cctgttgcgc caacaacttt ccggaaaatt tttcaatgat
     9841 gttctctccg ggtttgtcat ccaaataatc aataaatgaa tcgtgctttt ctgccgtttt
     9901 atctgtaagc ggctgaaacc aatgtgtata atgagttgca ccaagttcca ttgcccattt
     9961 tttcatccct tcggcaactt tgtcggcaat actgcgatcg agcgtggttc ctttttcaat
    10021 ggcttcatca atttctttta tagtttgttc cgaaaggaat ctggccattg tggatcttgt
    10081 aaaaacatac tttccaaaat actcggaggt taattcggaa ggtacttcca ctgcagcggc
    10141 ttttttcttg aaagcttcct caactgcttt aaatcttagt gatgacataa atttataatt
    10201 ttgaatttat tctgtaattt ttatcttaaa aattttctta ttgctaactt aatgttatta
    10261 tttctctgca aaattaaata aaatgataat ctcaatctgt gaaattcatg tttttttgaa
    10321 aaaatatttc taatacttac tgtttgaaac gattagttat aataaaaaga agaattataa
    10381 gagaatcaat agaaaaagtt atatattaaa agtttaagag ttttcttctc cgaataaatt
    10441 gagttgaagc caatactgaa aaaatttgaa caaagataga taaaagtttt cttatttttc
    10501 tttaagactt tgattcatat tgtttactga ttgattgata aaaggtgtaa gcatcaaaaa
    10561 atttcaactc tgcatcgaac gatctctatt cggatcatag aattttattt attcaattct
    10621 tctatctgac attttgttgt taaaattcga attattgttt cttaatgaaa ttttttcgta
    10681 gaaaacttca tttcaccgtt tctaaaaaac ttgttttaaa aagacagact atttcatctc
    10741 gaagtagtct gtctaccaaa actataaaac taaaaaacaa cacggttttg tccgcgtttt
    10801 ggtgaatacg tttgaaatct cgtgaaaaag ctgaatatgc gctcatgttc agcttatgtg
    10861 aaaaaattaa tcgaatgatg tataagcggt ttcgccatgc tgcgtttcat ccaaacctat
    10921 ggcttccaat ttgtcgggca cccgaactcc caaaatctta tccgaaactt taaacagcac
    10981 aaccgtaacg atacccgaat agagcagtac ggcaataact gcaattaact gtaccaaaag
    11041 ttgatgccaa tttcctgcta tagcgccact gtaggcagat tgaattgttg gagtcgccaa
    11101 cagtccggtc agtatcgagc cggtaatccc gccaattcca tgtacaccaa aagcatccag
    11161 tgaatcgtcg taacccattt ttggttttac gaaagccacc attgcaaaac aaatcaaaga
    11221 aaccaataca ccaatagcca atgctccgcc aacatttacg aatccggcag ccggcgtaat
    11281 ggctaccaat cctgcaactg ctcccgtact ggttccgaca atggttggtt ttccgtcgtt
    11341 aaaccagtcg agaattgccc atgtcaaagc tgcagctgca gcagcaatat gggtaaccat
    11401 aaaggcattt gcagccaatc catctgctgc cagcccactt ccggcattga agccaaacca
    11461 tcctatccac agcatggcag cacccattac aacgtaagta atgttgtgag gaggtaaagc
    11521 atgacccttg taatcacgtc tttttcccag catgattgcc gtaactatgg ctgcaatacc
    11581 cgcattgata tgtaccactg ttccacctgc aaaatcaaga gctcccagct tatacaacca
    11641 cccatcagac gaccaaaccc aatgagccac aggattgtaa acaaatagcg accacaaaac
    11701 ggtaaaaagc aagaaacctt tgaaattaat acgttcagcg aaagcaccaa tgataagtgc
    11761 gggtgtaatt accgcaaaca tacattggaa catcacaaaa accaaatgag gaatagttcc
    11821 aaccaaagtg ccgtctgctg cgatttgcga atgagaaata aagtaaggac ttaaatcatg
    11881 aatgccgata tttcttaaaa acgccccatc caatccgcca ataaaaggag aaagaaatcc
    11941 ttcagatttt ccgaatgaca tgctatatcc gcaaacaatc cattcaatgc tgattacggc
    12001 cacaataatc atacattgca tgaatatgtt gagtacattc tttctgcgta ccaagccacc
    12061 gtaaaaaaga gcaagtgccg gaatgctcat aaataataca agcgctgttg ccgtaatcat
    12121 ccacgccgta tctccggcat ctattttcgg ttcggcaact accgttgcag ccgatatttt
    12181 aatactgccc attaccaata gggccaacaa ggaaattata tattttttca tttttttata
    12241 cctttaaaaa ttaatacctt ataaacaatt gttttaaaaa acgaaactta tattccgttt
    12301 gaatctttat tgtacaaagc ttccggtccc gattcacgag tacgaatgcg gtaagtctgc
    12361 tcaatatcgg aaacaaaaat ttttccatct ccgggatcgc cagtccatgc cgaatccaaa
    12421 atggcattca ccgttttttc gagattgatg tcgcgcacta caatcgacaa catacgccgc
    12481 tgaatataac tcgactgaaa aatttgtcct cttaccacct gatcctcttt cgatttgcca
    12541 agtccggtaa tgtcccaata ggaaaaccac tcgatgccgg cctcataaag tgccatcttt
    12601 acatcttcaa atttagattt tctgatcact gcttcaattt ttttcataat cgtatgattt
    12661 taataaaatg ttgaattata aataattata atgataagcc aatggttaaa taatattgtt
    12721 ccgttgccgg atttaaaatg gcagaacctt tcagtggaag tttgaacgaa tcggttattt
    12781 ggatatctct tgtaacgctc aatccgatat ttactgcctg aaaacttcca tcaggagtgt
    12841 gccaaccgtc gcctgcacca gcaaataatc ttatgttttt aaagtcatat ccggcttcaa
    12901 aatacttgtc gccgccttta gaccctgcac ttccggcttc attgaaaatg taatttccag
    12961 cagcataaaa tcctgaaatt ttatatgaag cattgaattc ataggcatgt gaaccggtac
    13021 ttgcggaatg atcaaaaaat ttggatggag gaaaataata gtttgtcatt cccaatgata
    13081 aaccgaacgg aaatgcataa ttgacaaaca agtccatttc catgaaacca tcaatacctg
    13141 ccgagcccca cgaaccgaca gaaaaatttt gatggctgaa agtcagcgta ggttgaatgg
    13201 aagcatcgga gtatttaaca ccacgccaaa cgtacgaact aacaatatcg gtagatactg
    13261 taaagttttg agatttcgtt ataactggaa tagaaattgt gagggaacaa ataatcaata
    13321 actttgttgc tttcataact ttcaaatttt aattgtagaa tttttcattt ttccgtaatt
    13381 ttgagtttat atatacataa tgtttatttt caaataaaat acaatcaaaa tgttttattt
    13441 acatggcaaa attaatcata atgataaatt aaattcaaaa taaaatacat tttatttcaa
    13501 aaaatatttt ttacttacaa attgttttca gaattataaa aataatatca tattgttttg
    13561 ttttaactaa aatagttttc agaatgatat ttaataatat ttaaggacaa gcatttttta
    13621 ttttttatct ccaaatcgaa agtttgaaac tcatagcttg aaaaaggcaa aaaagcaaaa
    13681 gaattttttg aaaaacgcat taaaaaatat gaactaaaat aagcacattt catcttaaaa
    13741 aaatttcgat aaaacattct atatgagcaa tataaataaa atttcaatac tattttttgg
    13801 attgctttct caattattct gcattcaatt gcatgcagac aataatgtga aaatcattta
    13861 caacataaaa tacggacttg tcaatggcgg aaaagcaatg gttgaaaaga gagatacggt
    13921 atttggcggg aaaaaatgta tttattaccg attagaaggc aacacaaccg gattaacaga
    13981 taaaattttc aaggtgcacg acacttacga aagcatagtc gatgcgcata ctcatctccc
    14041 ttacgtggcg gtgagaaatg cgaaagaaaa atcttaccga tattataata aagtattttt
    14101 ttatcatcaa aaagattcaa ttttcagtga acgaaccgga ggaatgaaag ttccaccaca
    14161 attgattgat attctatcgg catttttcta tatggtaaat gtatacgaca tggacaccct
    14221 cacagaagga tacagtttga cattgcacac catcaacggg cacgatatac aaaagatgaa
    14281 agtgaaatat gtaaataatg aaattgtaca aacagacata ggaaacagtc tttgttatgt
    14341 cattgctccg gaagtgaaaa aagggaaatt gttgaaaagc tcttccggct ttaaatttta
    14401 cattgcacag aatgaaaaaa ttccttatca gattgaaatt gagacaaaag tggggaaaat
    14461 tgaggctatg ccaatagaaa tcataaggaa gtagaaaatt aaaaatacta ctgaagtcaa
    14521 aaagataaca ataatatcgt tttaccacat aaaatttgta tttttaccgc tgaattattt
    14581 acaaattaat taactatggt tcaaacagct tattctccca atcccgatcg ctatcaacat
    14641 tcggtcggat accgtttttg cggaaaaagc ggattgaagt tgccgcaaat ttcactcggc
    14701 ttgtggcaca actttggaga tgtggacgat gcggaagaag ccgaaaaaat gattcagttt
    14761 gcctttgatc acggcatcac acatttcgat ttggcaaaca attacggaac acctgccggc
    14821 agtgccgaaa taaatttcgg aaaaatattg aaaaaaaatt tcgcttctca tcgtgatgaa
    14881 atggttatca gtaccaaagc cggacatgaa atgtggccag gcccttatgg cgatggcggt
    14941 tcgcgaaaat atctgataag cagtctccac caaagtctga aaagaatgaa tctggattat
    15001 gtggatattt tttattcgca ccgatacgat ccgaatacac ccattgagga aactatggga
    15061 gccctttccg atatggtaaa acaaggaaaa gctctttaca ttgggctttc aaaatatcct
    15121 gaagaaaaag cccgggaagc attccggatt ttacgcgaaa acggcacacc ctgtttaatc
    15181 tatcaagatc ggtacaatat tttcagccga aatattgaac gctcacactt ccctttattg
    15241 aaggaaaacg gaatcggatt tattgctttt tctccattgg ctcagggaat tttaaccgaa
    15301 aaatatttgc acggaattcc tgaacattca agagctgctt tgtcctatgg ctttttacaa
    15361 aaatcgcaaa tcacgcccga agtattggcc aaagtgcaag aactgaacca aatggctcat
    15421 caaagaggtc aaactatggc acaaatggcg ctggcttggt gtttgagaac ttccgacgtc
    15481 aattcggtta tcattggtgc cagctcggta aatcaaattc aggaaaacat tgatgctttg
    15541 gaaaatattt cgttcaccga cgatgaactc tggagaatcg gtgaattggg agcttagcgc
    15601 atcaattacc ctgactcaat aaggaaattt aactgcaaaa tgaaaatttt caaagcattc
    15661 atctttttcg tattgctcac atgggtcttc atcggttgcg actactcatc aagatcttcc
    15721 attcctgacg ccacggtgaa ttttcagctc aacatgctcg tcgcacctta caacacattt
    15781 aatacgccaa attcatatct gattttcgac aaacgtatca acgcaattga ccgaattggt
    15841 tacggaggga ttttactcta tatcgactac gaagggaaat attgcgcctt cgacctcgct
    15901 tgcccttatg aagtaaaccc aaatgttaaa gttacgccaa atgatgaagg aaaagctgta
    15961 tgcgaaaaat gtggaagtgt ttttgaactt ctttacggta ttggcgctcc catttccgga
    16021 cctgccaaag aaccactgaa acgttaccat gtgtatttgt caaacaactg gctttacgtt
    16081 acaaactgac aatcgagagt tttaaattat ttccaacttt tttgaccgga aaaaacgccg
    16141 gaaacagccg ttattagtac ataaaaagga taaatcagcg aaagcagaaa agcataaaac
    16201 caaactgaac gtaagtcgaa aaattttcgt accgaataaa gaaaataacc gtcaatcaga
    16261 tatttgacca taaaacaaac caaaaaatat ttccacattc tcggcgttat taaagacaaa
    16321 ataaaaaggc acagcagaaa aaaactcgtc aaaaaaacca atagagctac agtaataaca
    16381 tctttatctc gataagcaga aaatttggaa gaccatcgct tgcgctgtcg aaaaaaatcg
    16441 cgcaaatttt cggcagtttt tgtttctacc atcgccaaag tcgattttaa aaatctgatt
    16501 tctcccttac tttttttcac actctccaac aaaaacatgt catctcccga aagcaattca
    16561 ttatgaagat catttacgtg aatcgaataa atcttttttg gaaaagcaag attcgctcca
    16621 ttacacaaga tcgatttccc aaaagctgct gctcctgctc cggaagccac caaactggca
    16681 aattcaaact tttggagttg aggaaaaatt tttgaattac tttttagctt aaccggagca
    16741 ataattaaat cgggacgata ttgaaaataa aaattacaaa tagtctcaat ccaactttca
    16801 caaggttcac aatcggcatc ggttgtaatc atcagttccc cgtttgcaat atttacggct
    16861 ttctgcaaag catttttctt tcctttaatt tctgatggaa aaaacttcat attggaaaat
    16921 gctgacgaaa attcctgcat tattttatag gtcccatccg ttgaatgatc atcgaccagc
    16981 accagttcaa aatcggaaaa agtctgacgc aacaaagccg aaagcaaccg aggaagatgc
    17041 tcttcttcat tgtggcaact gacgaccaca gaaacaggaa attttccatt cattcgatga
    17101 ggagaaagtt caaattcaga caatcttttc caacctatga tgaaaaaaat caccataagc
    17161 aggtaagaac caaccaaaat aaaacaacca attaggataa aattcattgt tcaaaaaatg
    17221 atttaaacca attaattaat agacctttta tgagaataac agataaaaag ttctttttca
    17281 aaatatttga taaaagaact ttttttgctt tgatgaaaca aatgctttac tttgattttc
    17341 cttttatcaa aagccacaaa aatataacat tttactgaaa aacaaagaca aacaaattat
    17401 gttaaaaatt aaacaggttg tcaataagtt gaaaatgaaa aagggtaatt tgtttatttt
    17461 caacgcaaaa acaccctctc ccacaaaata agtgaaagag ggtgtttttt aaaagaaaat
    17521 agacctttta tcagtcgagc acttttatat cgtaattcgg ctcgttgctg atttcgtcaa
    17581 ccaactgatt gtaaataact ttaccatttt catcgatggc tactacggca cgagccatta
    17641 gtcctttcag cgggccatca accagcagaa ccccataatc ggtagcaaaa gtattgtatc
    17701 tgaaatcgga agccgtgata acattgtcaa gtccttctgc tccacaaaat ctgctttggg
    17761 caaagggcaa atcctttgaa acgcaaatta ccacaacatt gtttttcccg gaaacccact
    17821 gattgaattt tctaacggaa gttgcacaag tgctggtatc caagctggga aaaatattca
    17881 gcacgacgcg tttgccggca aaatctttca agtgaatttc ctgaagatct tttccaacca
    17941 atgtgaaatc gggagctttg gaacccactg caggcaattc gccattggta tgtacgtctg
    18001 ttcccgcaaa tttaactgta gccataaaat taagtattaa aatgtttaaa aattattttc
    18061 attttaaact aataacaaag ctatgacgtt ttttgtttaa atttgtgtgg aaaaattttt
    18121 aacaatgaaa ttatctctgc tccataaact tggttttttt gtaagcattg tttttctact
    18181 cctttcctgt aaagatagcc atgcctatcg agtagattca gccttttcca cttatttgta
    18241 cacctttcag tccgaagccg caaaaagagg tcaggttttt aattttcaac aagaaggttt
    18301 gatcatggaa tttgccgatt taactgacaa caaagcagga ctttgtcatt atgaaaaacc
    18361 gatacgaata gaaattgacc gaacttactg gaatgatatt tcaaaatatg ccggggctga
    18421 atatatgaaa gaagatgtta tttttcacga gttgggacat ggttttttgg gccgtgaaca
    18481 tttgaatgat gtgttggaaa atggcgattg gaaatcaatg atgtgtggag gccagaaggt
    18541 gaacaataga gcatggaata taaattatcg cggcatcaga agagcctatt atctcgatga
    18601 gcttttcaat caaaataccc cttctcctgt attttctaca gaaaattttt cgatagatac
    18661 ttccgcctat actctcaaag ttcgtaaatc tttcgataca gcgcttgatg ccggttggaa
    18721 aatcgaaaac acttccgagt atgaaacttc catcgataat cgccgtctga aatttgagtc
    18781 aaaagttgat cctgtttatt ttgtttatgc caaaacgaat gtcgatgtac aatcagattt
    18841 tatttatcag cttcagatag aatgtaagag caagaacgat tccgaccagt atggtttgat
    18901 ttttggttca gtgcccgacc agggtgcatc ttacgtacaa aaatcgctgg agtattttac
    18961 cattaataat ttgcagaaaa tgtatatggg aaatacaaca tggtacagtt tttataccga
    19021 attaaccaag ccatgcatta ttccatccgg aaataaccgg atgaaaatag taaaatccgg
    19081 cgaaatgctt tattatttta tcaatgatgt gtattgttac tgttcagaaa tggaagtgaa
    19141 agacagcggt ttttattttg gattcattgt tccgccaaag agtacggttt ggttggacaa
    19201 tttaatcgta tctcagcaaa tcacttcaca gagtgcagta tcaaaagttc gagaattgcc
    19261 tgtatttgat gctcaagttg tttctgaacc gaaatttagg atgaagtgta ttaaaggcaa
    19321 ataaaaatta gaagaaaaac ttttttgttt aaaaccaaca aattcagtca ttatgtcaaa
    19381 atttgtaata taccaggttt taccgcgtct tttcggtaat tataattcca acaacaaata
    19441 caatggaagc atagaagaaa atggatgcgg aaaatttaac agctttacaa caaaagccct
    19501 gaaagagata aaagacttgg gagttaccca tatttggtac accggaattt ttgagcatgc
    19561 tacccaaaca gattattctg cttacggcat cagaaaagat tttccttgtc tggtaaaagg
    19621 gaaagcaggg tctccttatg ccgtaaaaga ttactacgat gtcgatcccg atttggctga
    19681 aaacgtcccc gacagaatga aagaatttga agatttgatt caccgaaccc acaaggaagg
    19741 catgaaagtt ttgattgatt ttgtgcctaa tcacgtggcg cgacaatatt tttcggacgc
    19801 caagccagaa aatgtcgttg acttgggcga acatgatcac ccggaatggg ctttttctcc
    19861 gctcaataat ttttattatt tgcccggtca acccttccgt cccaattttg atattgatgg
    19921 ctacaatgaa tttccggcaa aagttaccgg aaacgaccaa ttcacccctt ctccaacgct
    19981 gaacgactgg tacgatacgg tgaagctgaa ttatggcata aattatatgg aaggaatgca
    20041 aaaacaattc gatcctatcc ccgatacatg gaataaaatg ttggatattt tgcttttctg
    20101 ggcatcgaag aaagttgacg gctttcgttg cgacatggcc gaaatggtgc cggtagaatt
    20161 ttggaattat gccatcagca gagtaaagga aaaatatccg aaaattattt ttattgcaga
    20221 aatctataaa ccggccgagt atcggaatta tctggcaaac gggaaattcg attatcttta
    20281 cgataaagta ggtatgtacg aaacgcttcg aaacgtaacc agccgaaatt atcccgcccg
    20341 caacatcact tatgcttggc aacagctgaa cggtatcgaa gatcacatgc ttaattttat
    20401 ggagaatcat gatgaacatc ggatcgcttc cggatttttt gcaggcgatg gcagttatgc
    20461 gcagccggct atgatagtgg cagcaactct caccaaagct cccgttatga tttatttcgg
    20521 tcaggaactt ggtgaattgg gaatggataa cgaaggatac agcggactcg acggaaaaac
    20581 aactattttc gattattggg gagtaaagtc cgtgcaggca tgggcaaaca atggaaaatt
    20641 tgatggagaa aatttaactg cagaacaaaa ggaactgcga cagttttatc aaaagttgtt
    20701 gaatattacg ctgcaagaaa aggctataac ggaaggggta atgtacgatt tggaatatgc
    20761 caatttcaac aatcctcatt tcgatacaca cgaacaattt gcctacattc gaaagttcaa
    20821 agacgaaatg ctgctgtttg ttctgaattt cgacgataag gaactttaca ccgaagtgcg
    20881 tattcctttg gaggcttttc aatacttgga acttatgccg ggttgtcggt acaaagcagt
    20941 caatttactt gatgaggtgg aaacgattga acaaattaca ctcatacccg atatgatttt
    21001 taaaatttcc atcccggctt ggaaaggtaa aatttttaaa ttcaaaaagc tgtgaatcag
    21061 gaagtttttg ttataaattt ttttgacaaa ataatggaaa tggaatattt tttgatgaat
    21121 taaaactgac aattattctt gttttactta taaatctgta cactatgaaa gcttattttg
    21181 gcttgtttgc aggcatggct ttattgctga tttcctgctc cggagttaaa accactcccg
    21241 aaagtgttaa atctcttacc caaaaagtgg aaaataaaaa ttttacggtg aaagttcagt
    21301 atgctcaacc tctctcagga aaacagattt ttttgacgtc cgaatatgta ctgacagtta
    21361 aaaatgactc cgccgaagca tttttgccct atttcggagt ggcttattca gctccctatg
    21421 gtggcgatgg cggtattcgt ttcaatgaac gcatgaccga ctttagcata atgcccaata
    21481 aaagaaacaa tggttgggat attcatttta aagtcaaagc gccggaagat tactatgaat
    21541 tttttctgaa aatcttcaac gatgggagtg ccaattttac ggtaaattca aatagacggc
    21601 aatcaatttc gtttatggga gaagtgaaaa attagctaat gctaattgta gaaacttctt
    21661 acctcacggt cgaagaagat atgcaaatta accctatatg gaatgcttct gcttgaaatt
    21721 tgaaaaatat cagaaaaggt caaattcttt atttttttga ttgaatgtgt ccaaaaattt
    21781 tataagcgtc ccatttaatt aaataaatgg agtttttaaa aattaattgt ttagtcaacg
    21841 taaatcctcc gttcagtcag aattattttt aaaactgctg aaatagagga tagaaagtga
    21901 agaattgtta ttttaggaaa ctgtttgata acagagagcg ttgataaaat tttttcctca
    21961 ttcgacatta aaaatcattt taaaaaacac atcatttcaa taaacttttg ttcaattggg
    22021 ttgtttacac aatgataaga tgaaaaaagt tttgcaatgt tattttgtat tcaagacaat
    22081 gcaataaatt cgatttttta aaacgtaaaa acacaaataa ttgtttatca atcaaatcaa
    22141 ataaaataaa ataaaataaa aatataaaat ggctgaacaa aaggtcttat ctacaaaaaa
    22201 taatattgaa gggcaaatgc tggtaatatt cggttcaaat ggagatttag ccaggcgcaa
    22261 gttgctgcct tcaatatttc aactgcatgc ggatcgtctt ctacccgaaa gttttgccgt
    22321 aattggaacc ggcagtcagg aaaaggaaca atccgttttt ttgcaggaag tggaagaaag
    22381 tctgaaaaaa tatgtaaaaa gcgattcggt aaacgatgat cagacagtga gggaattttt
    22441 gaaaaaagtc tattacttgc ccgtcagaaa ccaggtggat gaagatttcg ggaaactgaa
    22501 agaatatctt cattcccttt cggcaaaact aaatattccg cgtaatatta tttactattt
    22561 ttctatccct ccttttctat acgaagtggt tgcttccaat ttggtaaaat atggactgaa
    22621 cagacaaaaa gacggttgga aaaggattat cgtggaaaaa ccgtttggtt acagctatga
    22681 aacagcattg gacttggaaa aaaaacttca tgccggcttt caggaagatc aaatttaccg
    22741 tatcgaccat tatctgggaa aagaaacggt gcaaaacatt atggttacac gttttgccaa
    22801 cggttttttt gaaccgatat ggaacagcaa atatgtggac agagtagaaa taacggctgc
    22861 cgagaaaatt ggcgttggca atagaggcgg atattacgat actgcgggtg cacttcgcga
    22921 catggtgcaa aatcatttgt tgcaggtaat ggctattgtt gcaatggaac cgcctgccgt
    22981 ttttgattcc gattccatcc gaaacgaaac ggttaaggtg cttcaagctt tgcgacccat
    23041 tgatgaagaa catgttgccg aaaatgtggt gcgtggccag tatatcgaaa ctatggtaga
    23101 cgacgtgctg caaaaaggct atcgtcagga gaagggagtt gcgtccaatt ccagaaccga
    23161 aacgtatgtt gccatgaaac tttttatcga caactggcgt tgggcagacg taccatttta
    23221 tattcgcacc ggtaaacaat tacctgttca ggttaccgaa gtagtcattc atttaaaacc
    23281 ggctcctcat cagttgttca agcaacgttg cattactcag tccaccaata tgattatact
    23341 tcgtctcaat ccggatgcag gcattgccat cgatttcggt atgaaaattc ccggcgctgg
    23401 ctacaaagtt caaaatgtca acatggcatt tcattattcc gacttggcgc attcgaaaat
    23461 tccggaagct tatgaaagac tcattttgga ctgcatgatg ggagattcca cactttatgc
    23521 ccgagccgat gcactgagag caagctggaa gtttgtagat cctattttga atgcttggaa
    23581 aaataatcct gaaattccac tctattttta cgaatgcaat acgtggggac ccaatgaagc
    23641 caatgccctt tttggcgaca aacgtttgaa atggaggcct ccattcaaca aatttaaaac
    23701 aaccgaagaa taaattatca attctcattt ctttatacaa agttcgcaat gaagcaatat
    23761 gatattcatg tttacaatac gccggaagat acagcaaaag ctattgctca gcttatttcg
    23821 aatcggataa acgaaaaaca aaattcatta tcatccttta atttggctat ttcgggagga
    23881 aaaactccac aaacactttt taatattctg gctaatgatt ttgcggaaaa aattccgtgg
    23941 caccaacttc gcctgttttg ggttgacgaa cgttgcgtcc ctccgacgca tccggaaagc
    24001 aactttggaa tgactaatga aacgctgctg aaaaaagttc ctcttcctaa aaaaaacgtt
    24061 tttcgtatga agggagaaac tgttccagaa gaagaagcca ttcattatca atcgcttctg
    24121 acaaaagaac ttccatcaaa aaacggaatt cccgttttcg acctgatttt gttgggaatg
    24181 ggaaatgatg ggcataccgc ttccattttt ccaaaagaca taaatttgat tgacagtcat
    24241 ttatctgtag cggttaccca acatcccgac acaggacaaa aaagaataac gctaactggg
    24301 cctgtgctga aacaggcaga cgaaataata ttttttgtta ccggtgcttc aaaagcgaca
    24361 attttaaagg aaatcgttct gaatgagcct cctgcaaaaa aatatccggc ttttcatatc
    24421 tattcggtag aaggaaaaac ttctttttat ctggacaaag aagccgcagc tcttctaccc
    24481 aaagaattgc cattgttgta atattttttg tttattcatt gatttcaaaa atccgccaat
    24541 tcaaattttg attgataaaa catttcaact tgatagcaaa gaaagtcaaa aaatttattc
    24601 cgtagctttg acttccagaa tatcctttag tcgggtagtg taaaaaggag aaagtttttc
    24661 gtttttcaat ttccagttgc gcccaaaacc ttgtgcagca atacgtactt tgtgtttccc
    24721 gtaggtagca tttaagctat ccattacctg catggcttta ccgaattttt ccctgtcgcg
    24781 cgtatcgaac aaatcggtct gaagcggagt ctccggaaca atatcgccta caacaactcc
    24841 tgcttttttg tacagataac cttcggcaaa aatagctttc aaagcctgca gtgcatgatg
    24901 aatcaattcg gctgaatcgt tggttggaac aggtagagga acggttttct ggttaaaata
    24961 ttgcaattgt gaggttgaga aagggttggt atgaataaaa accgtcagca gcatagcttt
    25021 tgaatgttgt tttctcaact tttcggcaca gttggctgca aaatttgcta ccgcttccga
    25081 tacgggagca tattcggtga gtctttcacc aaaacttcga gaagtacaaa tagatttttt
    25141 ggcagctacg tgttccattt caatgcacgg attcccttgc aattcgagcc aagtacgttc
    25201 gcccgttaca ctcatatttc ggcgtaccca ggctttcgat ttttgagtaa aatcccaagc
    25261 cgtacgaata ccctgatatg ccagcatttt ggaatattgt ctgccaattc cccaaacatc
    25321 ttcaatggca gtgagttgga gtgctttctc acgtttttcg tcggtatcaa ttaaacaaag
    25381 tcccttgtaa tctataaatt ttttggcaaa tttattggct acttttgcca gtgttttggt
    25441 aggagcaata cccatactga caggaatgcc ggtgttgcgt acggtagttt ccacaatttt
    25501 tcttcccaat tcctttaaat tttgcttttc aaacccatct aacaacaaaa aggcttcatc
    25561 tatggaatac acttccacat cgggcacaaa agatgccaat gtagtcatca ccctgtgaga
    25621 catatcaccg tacaaaacat aattggaaga aaaaacggca acattttgtt tttctatcag
    25681 ttccttcaat tgataggcag gttgtcccat cggtattccc agtgctttgg cttcgttgga
    25741 acgggaaatc acacatccgt cgttgttgga caataccaca acgggacgac ctatgagcga
    25801 cggattaaaa acgcgttcac aggagacata aaaattattg caatctacca acccaaacat
    25861 tttgcacatt tttttcttct tttccctcaa tttcgggaaa aatttcttgg acttatttcg
    25921 tttttccctg cttttgtttg tgatgcttta tggaatacgt aacaattccc caaatgcaga
    25981 aatgattttc ctccgttacc ttaataggct tgaatttcgg attagccggc attaaataca
    26041 cagcgtcctt ttccatgtgg atatatttca gtgtaaattc gccatccaga aaacataccg
    26101 ccatatcgcc atcctgcggt tcgatcgact tgtcgataat cagaatatct ccgtcttcaa
    26161 taccggcatc tttcatcgat gttcccttta cccgtcccaa aaaagtactg gaaggattgg
    26221 caataagttc acgattcaaa tcaattttca catcgatgta atcctgcgcc ggactcggaa
    26281 atcctgctgc aattcctcca tctgcaatag gcagataaaa agcagactcc gtatttaccg
    26341 agtaaaaatc aatataacct gatgttgcga ttttatcagc tttcataaat ttttgttgac
    26401 atgaataaat cggtttttgt ttgttctttc aacacaaagt aacagatttt tttgaaaaaa
    26461 aaggaaagag caaagtaaaa gaaagcataa aaattgaaaa aatcaacaat ttattgtatg
    26521 tcaatgcgat aaaaaattag gagttactct tttaatagtc cggtttacgc ggcaagatta
    26581 taaatgtttg tgaatagaaa tagaaacaat gcttcgataa ataattctct tataaaaagt
    26641 tagaaattga ttgcacaaat ggttatccta cttttcaaat taagatcagg aaatgtcata
    26701 atattgagaa acccgtatga ttacatacac aaacacgaat gaatataatt ttagacatgt
    26761 gggttccatc agaccattaa aaaataaatc atattctgat gaaaagaagt ttaaaaatta
    26821 gagtttttca tcccaaaaaa tataatgtac tatagtctga agcgttcgca aacgggcttg
    26881 gataagttgt tttttaccta gatacttttt tacaagcaag tgctttttgc aggtgagccc
    26941 atgtttttgc aggtgagcca tttctatttc tatttctatt tcaatatcca ataaaaagag
    27001 actgtcccaa cttttgagac agccccattt ttgagcggca aacgggactc gaacccgcga
    27061 cctttagctt gggaagctaa agctctacca actgagctac tgccgcaaat agaaatacaa
    27121 aagtaaatta aattttataa acgacaagaa gttttcaaaa aatcccttag atatggatta
    27181 taattctttt gttgtgttaa taacatattc cccgaaaaaa tttattttac gataccggag
    27241 aaacccataa atgccaaagc cagcaaaccg gcagtaagta atgctatggg tgctccttcc
    27301 attttttttg gaacttttat catgctcaat tgttctctaa tgcccgaaaa aagaattaat
    27361 gccaaaccga aaccgatgga tgtagatatg gcaaatacta cactttcaag taaattgaag
    27421 tttttctgaa ccaccaaaat agctacgcca agaattgcgc aattggtagt aattaaagga
    27481 agaaaaactc ctaaagcctg atatagcgcc ggggaaattt tttttaagat aatttcaacc
    27541 agttgtacca aagctgcaat aaccagaatg aaggtaatag tttgtaaaaa agcgatattg
    27601 taagcttcca gaatggattt ttgaatgata tatgtgaaaa tagtagcaat agtcattaca
    27661 aaaactactg ctccactcat tcccaaagcc gtattaattt ttttggatac ccccaagaat
    27721 ggacaaattc ctaaaaattg agaaagaaca atgttgttta caaagacggc actgatgaaa
    27781 attaaaatat aagtcatggc attgtaaata tttaatcagt tcaacaattt aatatgtttc
    27841 aaaaataaat ctatcaggtt ttatgctttt ctaatcttgt tcataattgc aatcagataa
    27901 gcgagagcaa taaaagctcc cggagccaga acaaatacca gcattccgta tttttcgggt
    27961 aatacactca gactgaaaat ttttcctgtt ccaagcaatt ctcttactat gcccaacaat
    28021 gtcaatgcaa aagtaaatcc caatccaatg ccaatgccat caagggacga atcgataact
    28081 gagttttttg atgcgaaagc ttcagccctg cctaatacga tacaattgac aactattaag
    28141 ggaataaaaa taccgaggct ttcaaaaagt gctggtaaat atgcctgcat aagcatttcc
    28201 acaactgtta caaatgtggc aataacaacc acataagcag ggatgcgcac tttgtcggga
    28261 ataagatttt ttaacagaga aatggccaca ttggacccaa ttagtacaaa tgaagtggcc
    28321 aaacccatac taaaaccgtt gattgcagaa gatgtggtag ccagcgttgg acacattccc
    28381 aatatcaata caaaaatagg attttcggtg attaaaccat tgagtatgat tttaactttt
    28441 gaattactca ttgttttatt ttttgcggat aataatcgta ataaatatat aaaaatatgt
    28501 aattacttaa tttatatttg atgctcccga tgaaacatca tgggtttttg gtgctttggg
    28561 attgttggca taagcctgat acgctttgtt cacggcttcc aaaaaagctc ttgagctaat
    28621 ggttgctgca gtgatggcgt caatttctcc tccatccttg ctgaccaaaa gacgatgttt
    28681 agcaggattt tttccacgaa tatcgtgttt ggtgttggac attttaaacc aatctaccat
    28741 ttttgtcccc aaaccgggag tttctttttg ttccaatacc gaataattta tgatatttcc
    28801 ttccttgtcg aaaccaatca taatttttat ttctcctccg aatccatttt ttgagttggc
    28861 ttctaccgca gcacctacaa aaacgttatt tttgtcatag gccttgtaca cattcagtgt
    28921 atcgacatta tttactggaa ctttgatggc agtatccaaa tgttcaaaag tcggcaatac
    28981 ttctttcagt gctttttgta atttggcagt ctttgacttt tctattggtt ctttggtgag
    29041 tgtatatact gagccgagtg ttaaagccga aaaaactgtt atcgacaaaa gtgaaatcac
    29101 catattttta aaagaagatg ttaatttagc cattcttacg tacctcccca aaacgtttag
    29161 gtttaatata tgtattaatt agtggtgtaa acgcattcat gataaggata gcaaaagata
    29221 ctccttcggg ataggaacca aacattctga taacaacagt gataatacca atgccaatgc
    29281 cgtaaattgt cattcccatt tttgtcatgg gagaagtaac aaagtcggtg gccataaaga
    29341 tagctcccag cattaatccg cccgacaata aatgtattat tggggaaaca taggtctgag
    29401 gattaacaag atagaaaatg cctgtcatga taaatacagt tagtaaaata ctcacaggaa
    29461 tatgccaggt aatggtacgt ttaaagagta agtaaacaaa tcctaacagc aaagccaatg
    29521 cagaaacttc tcccatagat cctcccatat ttcctacaaa catatcgaac aaagagggta
    29581 tgtttcccaa atgttctttc aaagcactta aggctgtagc tgaggtttct gcgtcaaggt
    29641 aagcagtatt ccaagccata gggcgaggcc aagatgtcat ttgtacggga aaggaaatca
    29701 ataaaaaaac acgacccacc agggcgggat tgaaaggatt gtttcccagt cccccaaaac
    29761 tcattttgcc tacgccaatt gctaccaaag ctcctataac aacaatccat acgggcagat
    29821 tggaaggaag attaaaagat agcaatagtc cggttaaaac agctgaatag tcggaaacag
    29881 tattttctcc tttgattaaa aatttttgaa ttaagtattc aaaaattaca catgaaatca
    29941 ccgaggttac acttacaatg atagcgccaa ctccaaaata atacagagcc accagatatg
    30001 caggaatgag tgcgatcagt acactgacca tttcttttct gatggaattg ccactgtgta
    30061 cgtgtggggc aggcgaaacg attaatttat tcatattttt aatttttctg tcttatgatt
    30121 tttgtgtttt cctgtttcga atcatcgttc cgacggtaga ttttccataa cgaatgtaat
    30181 cgaccaaagg tctattggaa gggcaaataa aactgcatga accacattct atacaatcca
    30241 ttacacgatt ttcttccata ttttcccaca tctgtttttc tccctgattc ataagaagat
    30301 acggttctaa gcccatcggg cagtgaaaaa cacatttagc acagcgaatg caattaaata
    30361 cttttcctct tttcgattcc gaggaaggga taaacaatat ggaagatgtc cctttagcca
    30421 ccgacaggtc gagacttggc aatgcacgtc ccatcatagg gcctccggct acaacttttc
    30481 cagtatcctg aggcattcct cccgtttctt ctataatatg tgtaaaggga gttcctatac
    30541 gaacccaata gttacctggt tcgggtactg atttgccagt taccgtaatg attcgctcaa
    30601 taagcggttt atttttttgg acggcttcat acacggcaaa agttgtggct acgttttgaa
    30661 ctactgcgcc gacttcaata ggtaaagctc cactgggaac ttctcgtccc gttaatgcct
    30721 gaatcaattg tttttcactt ccctgtgggt acttgacttt gagaggaact acttctatgc
    30781 cttccattgt tttcgcccat ttttccaagt tgagcacagc atccatttta ttgctttcga
    30841 ttccaataat tgcttttttt actcgaattg ctttcatgag tatgctgatg ccgatgataa
    30901 tctcttctcc tttttccagc attagctgat ggtcgcaagt gagataaggt tcacattctg
    30961 ctccgttgat aataagtact tcagcttttt tcccctgagg aatagataat tttacgtgcg
    31021 ttgggaaagt tgctcctcct aatccaacta ttccggcttc tgcaactttt ttaattattt
    31081 cgtgaggctc aaggttacat actcttttta cttcaggcga caaatcaatt tcatcgagcc
    31141 aatcgtcacc tattacatca ataaatactg ccggaaattt atatccgaat gcatccattg
    31201 cattatcaat ttttgatact tttcccgata ctgaggaatg aacattggca gaaataaatc
    31261 catttgctcg tccaatcaat tgaccaactt ttactatttc acctctggat acttctgcta
    31321 cgctcggatt gcctgaataa tgacgaagtg gtataatcac ctgctggggc aacgagatta
    31381 tttttgtttt ttttccagct gaaaatttat tttccggggg gtggacaccg cccattttaa
    31441 atgtcttcat taaaagttta tgattttttc ttatctattt aaatcaaata aaaattgtgt
    31501 tgaaaaaact tatttctcag ttcctgttgt agaaggttcc tctatttttg ttttacgtgg
    31561 tggaaaattc aactccacga tagcattagt tgggcaaact tcaacgcatt tgcggcataa
    31621 tttacacttt tcggaatcga tataagccaa attgttcccc atttcaatgg cttcgtaggg
    31681 gcacacgttg aagcatttgg agcagccaat acaagccact ttgcaaactt ttttatttac
    31741 agctccctta tccttgttta cgcaattcac gtaaattctt ctcgatttag gaccttttcg
    31801 tcttagttca atgatgtttt tgggacatgc ttttacgcag gcgccacagg ctgtgcattt
    31861 atcttcatca acttcgggca acattgtttc gggattaata tggatagcat cgaaagtgca
    31921 agaaacttcg caatctccca gtcccaaaca accccaggaa caaccggttt cacctccata
    31981 taaattgtgt acaatcgtgc atgattttac accatcgtaa ctattggttt tttcacgcac
    32041 ttcgcaagtt cctccgcagc gaaccacagc tatggtagga actactgccg ctgctttctt
    32101 tcccaaaata tcggccactt tttccatagt agccgtacct cctaccggac aagataaatt
    32161 atctagtgta tcggctgtta cgcatgctgc tgcaaatgcc cggcaaccag ggaacccgca
    32221 accaccacag ttggcagccg gtaacaccgc ttccacttcg tcgatgcgtg catcttcttc
    32281 tacatgaaat ttttgggcaa taaaatatag aataactgcc gataaagcgc cgattcctcc
    32341 aagtgaaact actgatgcta atattaaatt cattgctgat ttatttgtga tttgatttta
    32401 ctaaagaatt ttttaggtaa gtgattcttt tttgacgtga aattcaaatt ttctttttaa
    32461 tttatggttg aataatgaaa gtatcccata atatggaaat atggagcaca gggctatgat
    32521 gccggaaatt gtttcgtttt gctgaaaata attggaaatt attaaggtaa gaacaatcag
    32581 caacaacggg aaaataaaag ccagcaaaac ggcttgaagt cccattgaat ttcgtccaaa
    32641 aattactact tgatcacctt cttttattga agaatcggtt acgactacat caacaatttt
    32701 tttttcctct tccattacag tacaggcact tttggcatga cacgcactgc atgccgactg
    32761 ctggatgata agtatttgag ctttactccc ttcaatatgt tgaactatgc cgatgtgttc
    32821 tatttgttgc attcaatttt ttttttgcaa tgttgacatt acaatttgtc ggcaaaaata
    32881 cggattattt atatttacat caaaaaaaat caataaaaaa tcgattttgt agagtcaacc
    32941 gggaatttga gaagttaaat tttttaaaag tatgagtccc ctcttaaatt tgattttttc
    33001 aaaaaaggga aaaataagat tatttgaaaa gcagcattaa atttttttgt tgtttcgata
    33061 ttttacaata atttgcaata taaaagttat ttttgttaca aaagtagttt tttgtaatgt
    33121 attaagcttc tttgagcggt ttgcactata tatttcagta ttcgaatgaa gtttatccac
    33181 aaagtgcgag aataagccca taagcggtgt ttgcaagtcg gcgataacgt gtcttgtcat
    33241 tggaataatg gtaacctaac tgaaaaatgg tcgcttcaac attgtttcta tggttaaatt
    33301 cttcttttgt ataatgtttc gtagtttttg tctcaaaaca gaagttcaca cctgctcaag
    33361 gttaaaataa cgatagttgc gattttctgt ttttttacgg cattttttat gaaggctcaa
    33421 ataactgccc gtcgctccag ccctgacctt cttttaaaat catcatcccc aacaaaactc
    33481 tgataaaagt ttactgaaat acaataatat atgaaaattg tcaaaaaact tttttgaata
    33541 tttttcctca tttttatttc aatgtcttaa aaaaactaat tctctttgag taacttttcg
    33601 gaggggaata ttgcattatt ttctgtttga tacaaaattt gtttggcata ctgaacaaca
    33661 tcaatccctt gtttttttct aaaaagagat gtattttttc tcgctgattg acgctgattc
    33721 ctttctgcgt ttatctgcgt tgcttatccg cgataatctg cgagaaatgt attttttctt
    33781 tctcgctgat tgccgctgat ttctttcgct gatgctgaac aaattcattt cattcctcta
    33841 aatgattgac tattcttttg attgaagcct caatgttgtc tgtattgaaa tttaccaaaa
    33901 tacctaattt tttttcggat aatttcagat aagtcagcaa ttgtttgtaa tggacatccg
    33961 tcaactgttc gacagatttt gtttcaataa tcactttatt ttcaaccaga atgtcgactc
    34021 gatatccaat ttccaatgca atatcttcat aaacaaaagg aagaccaact tgtgttttaa
    34081 cttcaagacc agcatttttt aattcataag ataacgctgc ttcatatgca gattccaata
    34141 agccaggacc aacattgtta tacacagcaa aaatagctcc tctaatttta taagatattt
    34201 cattttctgt cataagctat aaattttttc acgttgattg acgttaattt tctgtgtctc
    34261 tctgcgggtt tttaatctgc gataatctgc gagaacttta tttttttctc gctgatttac
    34321 actgatcttc tcacgccgat ttacgctgat cttctgcgcc catctgcgtg aaatatttct
    34381 ttcttttctc gcagatcaac gctgattttt ttctcgctga tttacgctga tcttttctct
    34441 ctaataacat taattttccg cgtccatttg cgctttttaa tctgggagaa tctgtgtgaa
    34501 atattattct ttttatcaga tcaacgctga atttaacaag ttttttggtt acaaccacaa
    34561 atatagctgc tttctcttat ttttttctgg ttataaccat aaaaatttct gcgtctatct
    34621 gcgggttttt aatctgcgat aatttgcgag agatgtattt tttctcgctg atttacgctg
    34681 attctctgcg tctatctgcg ggtttttaat ctgtgataat ctgcgagaac tttatttttt
    34741 ttctcgctga tttacgcaga tttctttcgc tgatgaacgc tgatttttta tactcatttt
    34801 taataataca tttttttgat tacatcaact accctttgga tatcttcatc tgttaatata
    34861 ggacccgaag gcaggcaaag cccgtcgtca aacaaccgcc cctctgtatt gtcgccgtaa
    34921 tagggacaac aggcaaacac cggctgcaag tgcatcggtt tccacagcgg gcgggtttcg
    34981 atgttc
//
LOCUS       DAOTAX010000017        31438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1173_length_31438_cov_29.941274, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000017 DAOTAX010000000
VERSION     DAOTAX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 31438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31438
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1173_length_31438_cov_29.941274"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLM04_03320"
     CDS             complement(<1..719)
                     /locus_tag="PLM04_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HON02141.1"
                     /translation="MKKFFPILLIFFIGFSGCSHAEKTIVVDGNGTGDFRNIQQAIDS
                     VRAFNPDGWVTIYIKNGCYKEKIEIPTHICSIRLIGENRDKTIISFDDHAKINNMGTF
                     KTYTLKICGNDMLVENLTVENAAAPVAQAVALHVEGDRIVFLNCRFLGNQDTVYAGRE
                     NSRQYFENCYIEGTTDFIFGPATCWFEKCQIYCKRNSFITAASTPQNIPYGFIFNNCT
                     VSLADSVNAVYLGRPWRPYAMT"
     gene            complement(733..2145)
                     /locus_tag="PLM04_03325"
     CDS             complement(733..2145)
                     /locus_tag="PLM04_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HON02142.1"
                     /translation="MKIFKELVFVCCLFTVGISASAQIPAFPGACGGGVFASGGRGGK
                     VLFVTSLADDNSEGTLRWAVNQKGARTILFKVSGIIPLTKTLTIKEPDLTIAGQSAPG
                     DGICIKNHDVFIAADNVIIRFIRFRLGNEILHNESDAVGGRDCKNVIIDHCSMSWSID
                     ECASFYTNEDFTLQWCIVSESLNNAGHAKGPHGYGGIWGGKNASFHHNLIAHNLSRNP
                     RFNGWRREGLKYNTSIDEERVDFRNNVIYDWGDNSSYGGENGKYNIVGNYFKYGPATK
                     PSVRFRITHVDMDSHPEMVSPGYGQYFIDKNFVYGCPECKKDNWECVSYAEGVNKQLC
                     KATHPFECTPIIEQSAEVAFKKVLQFAGASFRRDAVDKRIVKETQKGKATYKGSINGR
                     PGIIDSQNDVGGWPVYRSTEPYPDTNQDGIPDGWLEKNYPGKFATDINEEGYTYLEVY
                     LNSLVEKITKSEYTNALSNK"
     gene            complement(2157..3809)
                     /locus_tag="PLM04_03330"
     CDS             complement(2157..3809)
                     /locus_tag="PLM04_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON02143.1"
                     /translation="MLQIFAQIPAFPGAEGDGMYTTGGRGGKILYVTKLTDDGTTGTL
                     RWAINQSGARIIMFKVAGIIPLTSELRIKNGNLTIAGQSAPGDGICLKNYPVVIQASN
                     VIIRFIRFRMGDEAAAGNTSYSWDGADAIWGRNQSNIIIDHCSMSWSIDECSSFYDNK
                     NFTLQWCVIAESLRKSVHGKGTHGYAGIWGGAPATFHHNLLAFHDSRNPRFCGSRFSN
                     SPSTERVNFTNNVIYNWGSNSGYGGEGGYYNMMNNYYRSGIARSSGSTSYRIFSPNAD
                     DGTNSQPKGVWGHFHLSGNIMINTPSVTDNNWLGFQPVGTYSDTIKSEVPFDFPPYLR
                     LQRADSAYLSVTGFAGASLKRDAVDTRIIDNVLNGTVTVTQGSNGSTGGFIDSQADVG
                     GWPEYTYDPATVPTDTDGDGMPDDWETAKGLNPNDASDGSTKTIDGQYTNVEVYLNEL
                     AAPVVNRQYLDANTAVNEVYANSSLRIYPNPFKSGNLNIKAAEKINKVEFYTLSGAKI
                     LEKEMNGTEISFTMQQLNAGIYIVKVKMEDGIILPTLLIVEP"
     gene            complement(3868..7083)
                     /locus_tag="PLM04_03335"
     CDS             complement(3868..7083)
                     /locus_tag="PLM04_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HON02144.1"
                     /translation="MKKIFYFLLLFFFVGFSIIKAQTKIDVWDFGAAQFDPTIYNNML
                     DEATINSWYPGVTPGTTGKNLPSSFTVGILSWTSTSSTSDRLRTSNTNLTRYDSNGVP
                     VTVASTSETLTGYLYVNASAATGRYLSLNLNEDDEVTIYAKSQNGTGKMTFEYTSDNS
                     QKDIVSLTSSPEIYKFVAKKAGVYKIYDSVDKPFYYRILRKPATYVSVSGTFDLTNAS
                     DIPSGYTINFTNAAGKVWSVAPDASNNYSINLPAGYTYSLSLSNANGYIISNSDPLSV
                     SGNTTYNPVILKVTLYTLSGNITGLPSEKLAKLGLIFTPSDTRIYVPKPVVNASAGTY
                     SVQLEANCDYTISATGVNDYYISNNNVNISSDINTDIVFVAKPVYTVLLNINGLDATQ
                     LSKLSVTFSNLNESGYVYSFTDLNNIQLRDGVYSISCSGLDEYPVQLGLTSNLKVNGA
                     ETSKDLSFIPVTDWSFDDATITSGVTTAYKGMLFTGSVYNEKSKGHLVLTGNATAKVP
                     VSPGEKMIVYYYYSAKFTVDGGSEISTNSGSTNRIESHEFVYAESTPGYMTIANVTGT
                     TTYLTEIKMVKSVTYKPIITVGTDKEYQSINDALAAVRCMTRENTDTVKIMIDPGNYE
                     EMLVIDIPNVTLVNASTTPSIALLNKGVDIDENAVRITSYYGHGYNYFSMGTDQKWNA
                     DVLRVNKENGYTTYSNTGSGTTNGSYWNATVVVSASGFRADNIIFENSFNQYISRKES
                     EDVVQEWSTGGKGTRPTTYGSTDVQNKNFVERAAAIAFTASADRAILNKCRVVGRQDS
                     FYGAEGARIAVYKGVLMGATDYLFGGMTLTCYQTDLAMNTSEASSDVAYITAAQQNSA
                     RGFLMYECNITSAVPSVETASLYRSKPGYFGRPWQPTTSEVVFYSTTIETTNNPSFYG
                     QSLIVPTGWNNSLGGTSDKVYEYNTIEKSGVDNSASRVSWSKVLTEPVLSDGTEITTF
                     NFTKGTDNWDPINTLKSNDINASVSLQKKDDFVRIFSVGSKVFISNVKNPSKIKIYSV
                     NGSLSFVKEISGDDEISLDKGLWIVNVVNNESIKTAKVLIK"
     gene            complement(7122..8207)
                     /locus_tag="PLM04_03340"
     CDS             complement(7122..8207)
                     /locus_tag="PLM04_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HON02145.1"
                     /translation="MADSEMKRNPESWMLDFSKKLKWDYCNGLELQAFLKVWEKTGDE
                     KYFQYAKSYADTIIEENGQIKGYKLEEYNLDRINSGKFLFTLYEKTKDEKYKKAIELL
                     RSQINTQPRTSEGGFWHKKIYPHQMWLDGIYMASPFLAEYAYRFNEPALFDEVAFQIL
                     LIAKHCYDPATGLYYHGWDESRQQKWADPVKGTSPNFWSRSMGWYAMAIVDVLDFLPE
                     NHPKRPEIIKVLTDLCYSLDKYRDPKTGMWFQVTDKINEKGNYVESSGSAMFIYTWVK
                     GAQKGYLPKEFLKKGKKAYKQFVKTFIVENSDGTISLKNACAVAGLGGEKNYRDGSYE
                     YYISEPIRDNDPKAVAPFIMVSVLLNK"
     gene            complement(8333..9124)
                     /locus_tag="PLM04_03345"
     CDS             complement(8333..9124)
                     /locus_tag="PLM04_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HON02146.1"
                     /translation="MKFRFSAITILGVLMLFFLASAPKPVKVLMAGDSTMADKVLYKT
                     VFDSLTGKSYEEPFLERGWGQLLPELLNDKALVKNYARNGRSTRSFIEEGLWTELLNN
                     TEKGDFVIIQFGHNDESVEKTGRYTNPAQFRLNFVAFVAEVRLKGATPVLCTPVARRN
                     FDNTGRLVPTHGSYPEIIRSVARDYRVPMIDLEKLTSEWLQHEGVEGSKKYFHKIAPG
                     ISRLYPNGLDDNTHFNEAGARTVAEMFVKEVATQKIKGFEKIFKK"
     gene            complement(9121..10737)
                     /locus_tag="PLM04_03350"
     CDS             complement(9121..10737)
                     /locus_tag="PLM04_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HON02147.1"
                     /translation="MKFKSLFLMLASAILITANLNAKNPYEYLFKNLPFEMPKIQPPT
                     FKNNVVRIEDFGGKGDGKTLNTEAFKQAIDALARKGGGKLIVPAGVWFTGPIELKSNI
                     NLHLEKGALILFSKDFDLYPLVDTYFEGLATKRCQSPISGRNIENVAITGEGSINGSG
                     EAWRPLKKSKVTASEWKKVVNSGGVVKEGNYWFPSEKSYKGYSISDMNVPRAKLTDEE
                     WMEIRDFLRPVMVSFIQCKNVFLQGVLFENSPSWNIHPFMCENVIVDNIIVRNPDYAQ
                     NGDGIDVESCKNVIIVNSYFDVGDDAICIKSGKDEEGRRRAVPTENLIVHNCKVFKAH
                     GGFVVGSEMSGGVRNISVSCCQFLGTDVGLRFKSKRGRGGVVENIFVDGIYMFDILTD
                     SFLFDLYYGGKSATEAMEEGNDNQKDAVLMPVTEETPVFRNIFVKNIVSRNAHRAMFF
                     NGLPEMKISNIQVENAIITATYGAELNNSKDIVFKNIYVEPTIGPALRLNRIENFKAE
                     NFTYPEKLQNAIELKGEENENIIISNVKIK"
     gene            complement(10836..>11873)
                     /locus_tag="PLM04_03355"
     CDS             complement(10836..>11873)
                     /locus_tag="PLM04_03355"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4957 domain-containing protein"
                     /protein_id="HON02148.1"
                     /translation="SGLIEDTDYTARLMKDDKVRGSATFTTKIDLNAANTIVVHPDED
                     LVAAIQAAPSGSRIVLVASDDGSNEFLKSAGQTINIDKGISIRGYLKSNKPTLHMQFR
                     LTAENASLTVKDVILNGQSDADMRDHVVQLTQSVACGDILFKDCIITNYGKSLISGAS
                     RIAVSVRSFTIQNCKVSNILTSSADCIDVRSGCIENATLLNSTFYNCAPDRDFFRLDD
                     ASSSFPGKSTIIKVNRCTFSKVNNDAARRLLYVRFANNEITFTNNVVANSVGYYTNQT
                     ATNIVLFNKNNYFNAPNYLVYTGTALNVKTDNSGTYLQQNPGFVDEANGNLTITNATL
                     LEQGIGANISW"
     assembly_gap    11874..11973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11974..12598)
                     /locus_tag="PLM04_03360"
     CDS             complement(<11974..12598)
                     /locus_tag="PLM04_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HON02149.1"
                     /translation="MKKIFKNIPILFSLLIVFTVSSCKDELPEEITELVTDRLFSPVG
                     VEAKVINKTQVVLNWTKNPKAESYVIEVYENDSLTFAGSPVMIFTNITNDDIPDTIKS
                     GLIGATQYSVRIKAVGSEIAESKWSAVYFKTGEEQIMNAVSEESVSAHSVKLTWKAGE
                     TATKIILTAGQSTLEHVVTAEEIAAGEATLSGLIEDTDYTARLMKDDK"
     gene            complement(12622..14409)
                     /locus_tag="PLM04_03365"
     CDS             complement(12622..14409)
                     /locus_tag="PLM04_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HON02150.1"
                     /translation="MKKTIFISAILTLFLMGIMSSCEETLEAPTKSSLDEQVIFSTPV
                     LAEGALMGIHQSLAETNSYRGRYIPFYGINTDIEWYNSSENVSDDRAKLTGYDPLPTN
                     TQMNMENNAWAKMYEAIERANLCIRGLRLYGNVEKNPELAQILGEALTLRAMIYNDLI
                     KAWGDVPARFEPISPSTLYLPKSDRDVIYKQIIADLAEAENYVAWPNETAVTRTTERV
                     NKAFVKGLRARICLAAAGYSQRPDGTIRRSNDPELSVENLYPIVKQECIDVIESKTCK
                     LGTFEQNFRNLCQDNVTAGLESLYEIPFSDGRGRVLYTFGVKHTTTDKYTQQAQGGQN
                     GPLPYVYYDFDKDDIRRDITCVPYEWTNGKQVPRKLSSWCFGKLRYEWMKRVVTSTND
                     DGVNWQCMRYADIYLMAAEAINELDGPEAAAPYLREIRKRAFPNNPEKVDAYMASVTT
                     SKIAFFNAIVNERALEFCGESLRKADLIRWNLLSTKMNEAKAKLTRLANRQGEYADLP
                     EKIYYKTASDGESLIIYGLEHGHTDTEGAALNYESSKGWFVSNGVNTLTVDKINSLFQ
                     RDPDTRQFWPIWQVFIDSSNGLLKNDYGY"
     gene            complement(14431..17676)
                     /locus_tag="PLM04_03370"
     CDS             complement(14431..17676)
                     /locus_tag="PLM04_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02151.1"
                     /translation="MRTQKKFISILFFILVGFFVQVYGFTDQNNLIQQNERTIKGTVK
                     TTDGEPLIGVSVIVAGSPSAGTVTNLNGDFTITVPANATKLQFSYVGMKPLTVEIGNR
                     SVINVVMTEEAIGLNEVVAIGYGTVKRKDITGSVVSVSSDAIMNIPVSSPAEAISGKL
                     AGVQVTTTEGSPDAEIRIRVRGGGSITGSNKPLLIVDGFPVQSIDDISPYDIESIDIL
                     KDASSTAIYGSRGANGVVIITTKSGKEGKITANYNFYTAAKKIAKTLDVLSPSDYAKW
                     QYELALLKNDGDASSYEKYFGNYQDIDLYNNVPSNNWQDLVFGRTGTTFNHNLSINGG
                     DSKNKFAFSFSHIDDKAIMQMSSFKRDNINFKYTTNPSKNVSMNFSTRYINTKINGGG
                     MNEQKEVSSADSRLKYAMIYPPFPLKDLTSEAGTSDDTEIGNLYNPLVAISDNDRQQK
                     RNDLNMAGDITWKAFDNFKLKVEFGYENYRAEDQRFYGTTTYYIKNIPSSENQGYPAA
                     ELNNTFLNTFRNTNTVSYDFKKLLGTSHHLDVLLGEEYIFNSKQTLTNTIHGYPKSFT
                     STNAFRLTSQGTPYNIENYYSPDDILLSFFGRANYDYLSRYLMSVTFRADGSSKFSKG
                     NKWGYFPSAALAWRISSEPFMESTQSWLDDLKLRLSYGTAGNNNIPSGQMTQSFDSKS
                     TSWINNVASYWSPSKIMANPDLKWETTVTRNLGLDFVLLKSKLNGTIEGYYNTTSDLL
                     INFPVPGTGYDTQYRNMGETQNKGVEFTLNWIAIDKKNYGLSFSGNIGFNKNKINSLG
                     IMSDFGASSGWASTEIGNDYWIAVGGSVGQMYGYRSDGRYEVSDFEGYDSVKKKWILK
                     EGVVDCSSVIGSDALRPGAMKLKDITNDSIVNSKDYTIIGNANPLHTGGFTINGRLYG
                     FDLSANFNWSYGNDVYNANKIEYTSTSKYQYRNMLAIMADGKRWTNLKSDGTISNNPA
                     ELEAMNANTTMWSPYMSKYVFSDWAVEDGSFLRLNTLTLGYTLPVHLIRQIKIQQLRF
                     YVTGYNLYCWTNYSGFDPEVSTRRKTELTPSVDYSAYPKSRQVVFGLNLNF"
     gene            18486..19736
                     /locus_tag="PLM04_03375"
     CDS             18486..19736
                     /locus_tag="PLM04_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HON02152.1"
                     /translation="MSIKFRLILMNFLQYFIWGSWLISLGSYAGNILNFSGVQIGSIF
                     ASMGIVSLFMPGLLGIVADRWIPAHKMLGICHLLGSIFLIAASFQTQYMAFYSFMFIS
                     VMLYMPTISLSNTVAYNALEKNHYDIVKDFPPIRVWGTIGFICAMWTVDFTGFKFSQA
                     QLYVAATAALILGVYSFTLPPCTVNRSNEKKSVIDVLGLRAFTLFKHKKMAIFFIFSM
                     LLGASLQITNGFGDLFLRSFSEIPEYMNSFGVRHSVVLLSISQISETLFILTIPFFLK
                     KFGIKNVMLMSMFAWVFRFALFGMGNPGDRLWMLILSMIVYGMAFDFFNISGSLFVEQ
                     STDSSIRASAQGLFMIMTNGLGATFGSYLAGAIVDKFVTHAEVPNWSTAWYIFAGYAL
                     IVGILFAILFKYKHNTQEVTEKFH"
     gene            complement(19905..22496)
                     /gene="clpB"
                     /locus_tag="PLM04_03380"
     CDS             complement(19905..22496)
                     /gene="clpB"
                     /locus_tag="PLM04_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HON02153.1"
                     /translation="MNFNNFTIKSQEAIQEAVDIVQSKGQQAVETPHLLKGVLLKGED
                     VIQYLFGKMGVNLSMLNSVIDRMIETYPRVSGGEPYLGRETNAVLQKAIDISSKAGDE
                     FVALESILMALVVENNSVKKALNDAGVNEKSLSEAINELRKGSKVNSASAEDTYNSLN
                     KYAINLNERARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLA
                     HRIVRGDVPENLKSKQIYSLDMGAVIAGAKYKGEFEERLKSVVNEVVNSNGEIILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGDLRAIGATTLAEYQKYFESDKALERRFQIV
                     MVDEPDIPSSISILRGLKERYENHHKVRIKDDAIIAAVELSSRYITERFLPDKAIDLM
                     DEAAAKLRLEIDSVPEELDVIERNIKQLEIEREAIKRENDTKKLEQLSQEIANLKEQA
                     DALRAKWASEKSLIDKIQQAKIDIENLKFEAEKAEREGNYGKVAEIRYGKIKALESTI
                     AQYQEELAKIQGNNPLIKEEVDSEDIAEIVSRWTGIPVTKMLQSEKEKLLHLEEELHK
                     RVVGQDDAIQAVADAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEYLFNDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNVLLQVLDDGRLTDNKGRTVNFKNTIIIMTSNIGSSLIRENFEKINEYNHDEIIE
                     RTKNEVFELLKQTIRPEFLNRIDELIMFKPLDRSQIEDIVRLQLDGVRRMLEQNGIRL
                     ETTDRAVKFIADNGYDPQFGARPVKRAIQKFILNDLSKKIIAGEIVSNQVVVVDADEN
                     GIVFKNK"
     gene            22789..25512
                     /gene="ppdK"
                     /locus_tag="PLM04_03385"
     CDS             22789..25512
                     /gene="ppdK"
                     /locus_tag="PLM04_03385"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HON02154.1"
                     /translation="MDNKKRVYTFGNGQAEGSASMRNLLGGKGANLAEMNLLGIPVPP
                     GFTITTEVCTEYYALGKDKVVELLKPEVEKAMEGVEKLMRSKFGDKKNPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEIVEGLAEKTGNPRFAWDSYRRFIQMYGDVVLGLKPANKN
                     DIDPFEAVIEQLKEERGVKLDTELTVDDLKELVKRFKALVAERTGKEFPLSVYDQLWG
                     AICAVFDSWMNERAIFYRKMEGIPSEWGTAVTVQAMVFGNMGNTSATGVCFTRDAATG
                     EDVFNGEYLINAQGEDVVAGIRTPLQITKQGSQQWAKLTGISEEERKEKYPSMEEALP
                     EIYKELNEVQEKLEKHYKDMQDMEFTVQEGKLWLLQTRNAKRTGTAMVKIAIDMLHQG
                     IIDEQTALLRVDPLRLDELLHPIFDKNAVKKAKVIAKGLAASPGAASGKIVFNADDAA
                     EWAARGEKVIMVRIETSPEDLAGMAAAQGILTARGGMTSHAAVVARGMGKCCVSGAGS
                     LKIDYAKKTMEADGIVLREGDYISLNGSTGDVYLGQIPTQEAELDADFAELMTLADKY
                     SSMKVRTNADTPHDAAVARKFGAVGIGLCRTEHMFFEGEKIKAMREMILAENEEGRRK
                     ALGKILPYQQADFEGIFEVMDGFPVTVRLLDPPLHEFVPHDEKGQKEMAEAMGLPLQK
                     IQQRVEELREQNPMLGHRGCRLGNTYPEITEMQTRAILGAALKLKSKGIKIIPEIMVP
                     LVGIKKEFLEQERIIRETAEKLFAEKGDRIEYKVGTMIEVPRATLIADQIAENAEFFS
                     FGTNDLTQMTFGFSRDDIASFVPDYIEKKIIEKDPFQVLDQEGVGQLIKLGVEKGRST
                     RSDLKVGICGEHGGEPSSIEFCYRVGMNYVSCSPFRVPIARLAAAQAALKN"
     assembly_gap    25860..25959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25963..26319
                     /gene="rsfS"
                     /locus_tag="PLM04_03390"
     CDS             25963..26319
                     /gene="rsfS"
                     /locus_tag="PLM04_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HON02155.1"
                     /translation="MEQSEQIVNKIVEGIQEKKGKKVVIINLTGLKNAPCSYFVICEG
                     TTSIQVDAIGTSIKDTVREKMNIKPYAIDGLRNSEWVAMDYGSVIVHIFRPQIRNFYD
                     IEHLWEDAEITEIYDE"
     gene            26377..28380
                     /gene="ftsH"
                     /locus_tag="PLM04_03395"
     CDS             26377..28380
                     /gene="ftsH"
                     /locus_tag="PLM04_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HON02156.1"
                     /translation="METKSPQNSPQKPGKGPKFNITWLYVIIIGILIGSYLFSDTAPV
                     KEVPFSDFEKYMYNNKMEKIDVYSSKNIAEALIIKDSLKQVFGEKYEMYEKEPKIRTR
                     IPSAEEFSKIYQKAKEQYGLTAVVDYKESRNYVDIFLYSILPFLLLILFFVYMNRRMA
                     NQMGGGSGGGIFNVGKSKAQLFDKGDVANRITFKDVAGLEEAKQEVEEIVEFLKNPSK
                     YTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEADVPFFSISGSEFVEMFVGVGASRVR
                     DLFRQAKEKAPCIIFIDEIDAIGRARGKNPNFGSNDERENTLNQLLTEMDGFASNSGV
                     ILLAATNRADILDKALLRPGRFDRQIHVDLPDVHGRKEIFKVHLRPIKIDKSVDIDFL
                     AKQTPGFSGADIANVCNEAALIAARKNKSFVEKQDFLDAVDRIIGGLEKRTKITTKFE
                     KKSIAYHEAGHATISWMLEHASPLVKVTIVPRGEALGAAWYLPEERQLTVRAHMLDEM
                     CSTLGGRAAEELFLNQTSTGAINDLERVTKRAYAMVTYFGMSEKLPNLSYYDSSGSAE
                     YSFTKPYSEKTAELIDEEVKNIVSREYERAKEILREHAEGHNRLAELLIEREVIFSED
                     LERIFGKRPWASRSDELLAQNGENGKSSERMEDNTVENENKEE"
     gene            28361..29026
                     /locus_tag="PLM04_03400"
     CDS             28361..29026
                     /locus_tag="PLM04_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HON02157.1"
                     /translation="MKIKKNDLINEEELSSREIILKKISDILCTRTSETIFSEYFSHQ
                     IYREIAPDMLTCFMEEIKAVSGECFVHDSPSNLFVALKKFLLGKGFSKIHCRNEKITE
                     YLKSFDIPFSSSEADFEIMEVGITDCEFLVARTGSVIVSSFNSGRKMNIFPPVHIVIA
                     HRSQLVSYLDDAYRQILQKYKNNLPSLISTITGPSRTSDIEKTLVLGAHGPKQLLVFL
                     LDN"
     gene            29092..29904
                     /locus_tag="PLM04_03405"
     CDS             29092..29904
                     /locus_tag="PLM04_03405"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HON02158.1"
                     /translation="MNEMLKRAISGFIYVGLIVTSILVHPLAFAFVFGILTYCALHEF
                     YKITYRPSDIEVPISLASMGGLLLFVSTFLFKSGFSSPLIFSYYAIFVIWMFIRELFL
                     KKSNPIHNWAYFILGQVMVALPMSLLIFILFVDGYQPMLLLSIFILTWLNDTGAYLIG
                     VKFGRHRFFEHISPKKTWEGFFGGIFFSLTGGFILSLFIAELSIFQWLALCFITVLAA
                     TLGDLTESLLKRTLNIKDSGNLLPGHGGLLDRLDSMLLVSPFVLIFFLYIFV"
     gene            29993..31150
                     /locus_tag="PLM04_03410"
     CDS             29993..31150
                     /locus_tag="PLM04_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HON02159.1"
                     /translation="MEMNFDDIRCYTNEEVHEVLEKLTKEKSFMKVVSTAYPLLPKEI
                     IKERLLQYNNQQEFQKDFIFPFLQYLEENTTKGIELFGLEKINVSKAYLYLSNHRDIV
                     MDSSFFCKKLLENSIDTVEIAIGDNLLIYPWIEDFVKLNKSFIVKRGLNTRQTLESSK
                     LLSAYLLHTICEKNQSIWMAQREGRAKDSDDYTQESVLKMLNLSAKDNNFIQHILQFN
                     ICPLSISYEYDPCDYLKAKEFQLKRDFPDYKKSPTDDLENMVIGIMGYKGKVVFRITG
                     CINDELKKIEQKTSNRHEQISQTAQLIDLKIHANYEIYSVNKIAFDTLTGENRFAHEY
                     SNMERIDFEQYLMKQIDKIQLENKDEKFLRQKLLEMYANPLINHLKTLQHS"
BASE COUNT         9562 a         6136 c         5593 g         9947 t
ORIGIN      
        1 gtcatggcat aaggtcgcca cggacgtccc agataaacag cattgacaga atcggcaaga
       61 gaaacggtac aattgttgaa aataaatccg taagggatat tctgtggtgt actggctgct
      121 gtaatgaagg aatttcgttt gcagtaaatc tgacatttct caaaccaaca agtagccggt
      181 ccaaaaataa aatcggttgt tccttcaata taacagtttt caaaatactg acgtgaattt
      241 tcgcgtccgg cataaacagt atcctgattt cccaaaaaac ggcaattcag aaaaacgatt
      301 ctgtctcctt ccacatgcaa agctaccgct tgagccacag gtgcagcagc attttcaacc
      361 gtcaaatttt caaccagcat atcgttacca caaattttta acgtataggt tttaaatgtt
      421 cccatgttgt tgatttttgc atggtcgtca aaactgataa tagttttgtc tctgttttcc
      481 ccaatcaatc ggatactgca aatatgagtc ggaatttcga ttttctcctt gtaacatccg
      541 ttttttatgt aaatggtaac ccagccatcc ggattaaaag cccgaacaga atcaatggct
      601 tgctgaatgt ttctaaaatc gcctgtgccg tttccgtcca ccacaatagt cttctcggca
      661 tgggagcatc cagaaaaacc aatgaaaaaa atcaacaata ttggaaaaaa ctttttcatt
      721 tttatccaca atttatttat tggacaaagc attggtatat tccgatttgg tgattttttc
      781 aaccaaactg ttcaaataaa cttccaaata agtataacct tcttcgttta tatcggtagc
      841 aaatttgccg ggataatttt tttcgagcca tccatcagga atcccatcct gattggtgtc
      901 aggatagggc tctgtagaac gataaacagg ccagccaccc acatcatttt gagaatcgat
      961 aataccgggt ctgccattaa tcgaaccttt ataagttgct tttcctttct gcgtttcttt
     1021 cacaattcgt ttatccacag catctcttct gaatgaagct cctgcaaatt gcaatacttt
     1081 tttgaaagca acctccgcag attgttcaat aataggcgta cattcaaaag gatgtgttgc
     1141 cttacaaagc tgtttgttaa caccttcggc ataagacaca cattcccagt tgtctttttt
     1201 acattctggg caaccataca caaaattctt atcaataaaa tattgtccat agccgggaga
     1261 aaccatttca gggtgactat ccatatccac atgcgtaatt ctgaatctga ccgatggttt
     1321 ggtggcaggt ccatatttga aataatttcc aacaatgttg tattttccgt tttcgccgcc
     1381 ataagaacta ttgtcgcccc agtcgtaaat cacattattt ctgaaatcga cgcgttcttc
     1441 atcaatggaa gtattgtatt tcaatccttc ccgtctccaa ccgttaaatc gtggattgcg
     1501 acttaagtta tgcgcaatca gattgtgatg aaaacttgca tttttgccac cccaaattcc
     1561 gccgtatcca tgtggacctt ttgcatgtcc ggcattattc aaggattctg aaacaataca
     1621 ccattgcaag gtgaaatctt cgtttgtgta aaaagaagca cactcatcaa tgctccaact
     1681 catggagcaa tggtctatta tcacgttttt acaatccctt ccaccaacag catcgctttc
     1741 attgtgcaat atttcattcc ctaatctaaa gcgaataaaa cgaataataa cgttatcagc
     1801 cgctataaaa acatcatgat ttttaataca aattccgtcg ccgggtgcac tttgcccggc
     1861 tattgtcaaa tcaggttctt tgatggttaa tgtttttgtc aacggaatga ttcccgaaac
     1921 cttaaaaaga atggttcttg cacctttttg attgacagcc caacgcagcg taccttccga
     1981 attatcgtcg gcaagcgaag ttacaaaaag cacttttcct ccccgtccac cggaagcaaa
     2041 tactccgcct ccacaagctc cgggaaaggc cggtatctga gcggaagcag aaataccaac
     2101 cgtaaaaaga caacacacaa aaaccaattc tttaaagatt ttcattttga aaaatattat
     2161 ggttcaacta tcaaaagtgt tggaagaatt attccgtctt ccattttaac ttttacaata
     2221 taaattccgg catttagctg ctgcatggtg aatgaaattt ctgttccatt catttccttt
     2281 tctaaaattt ttgcaccgct caaggtatag aattcaactt tattgatttt ttcagcagct
     2341 ttgatattca aatttcccga tttgaaagga ttgggataaa tgcgcaaaga agaatttgca
     2401 tatacttcat ttacagcagt atttgcatcc aaatattgtc gatttacgac tggtgcagcc
     2461 aattcgttca aataaacttc aacattagtg tattgtccgt caatcgtttt tgtacttcca
     2521 tctgaagcat catttggatt caagcccttt gccgtttccc aatcgtcagg catgccatcg
     2581 ccatcggtat ccgttggaac agttgccgga tcataagtat attcgggcca accaccaaca
     2641 tctgcctgag aatcaataaa accaccggtg cttccattgc ttccttgtgt aaccgtaaca
     2701 gttccattca gcacattatc aattattcgt gtatccactg catcgcgctt taaactggca
     2761 ccagcaaaac ccgttacgct caaataagca ctgtcggcac gttgcagtct cagataaggc
     2821 ggaaaatcaa aaggaacctc tgatttaatc gtatcggaat aagttccaac aggttgaaat
     2881 cccaaccagt tattatcggt aacactgggt gtattgatca taatgtttcc gcttaaatgg
     2941 aaatgtcccc aaacgccttt tggttggcta ttcgttccgt catcagcatt tggactgaaa
     3001 attctgtaac ttgtagaacc gctacttcgg gctatgccgg agcggtaata attattcatc
     3061 atgttataat agcctccttc acctccatag ccggaattgc ttccccaatt gtaaatgaca
     3121 ttatttgtaa aattaactct ttcagttgat ggactatttg aaaatctgct tccacaaaaa
     3181 cgtggattcc tgctatcgtg aaaagccaat aaattatgat gaaaagtagc aggcgccccg
     3241 ccccaaattc cagcataacc atgtgtacct tttccgtgaa cagattttct cagactttca
     3301 gctattacac accattgaag tgtgaagttc ttgttatcgt aaaaagatga acattcatca
     3361 atactccaac tcatggaaca atgatcaata atgatattgc tttgattacg cccccaaatg
     3421 gcatcggctc catcccaact gtaagaagtg tttcctgcgg ccgcttcatc gcccattcga
     3481 aaacgtataa aacgaataat cacgttactt gcctgtatta ccaccggata atttttcaga
     3541 caaattccat cacctggagc actttggccg gcaatggtca gattcccgtt ttttatcctg
     3601 agttcggaag taagcggaat aattccggca actttgaaca taatgattct tgcacccgac
     3661 tgattaatag cccagcgcaa tgtgcccgtt gtcccgtcat cggtgagttt ggttacataa
     3721 agaatttttc ctccgcgtcc gccggtagtg tacatgccgt cgccttcagc acccggaaaa
     3781 gccgggattt gggcaaatat ctgtaacaaa aaaatgtttg aaactaaaac acacaaaatt
     3841 gttaaaattc tatttttcct catcatttta ttttattaaa acttttgctg ttttaatgct
     3901 ttcgttattc accacattta caatccacaa tcctttatcc aatgaaattt catcatctcc
     3961 ggatatttcc ttaacaaaag ataaagagcc atttacggaa taaattttta ttttggaagg
     4021 attttttaca tttgaaataa agactttgct cccaactgaa aatatccgaa caaaatcatc
     4081 tttcttttgc agtgaaacac ttgcattaat atcattagat tttaaagtgt ttattggatc
     4141 ccaattatca gttccttttg taaaattgaa agtagtaatt tctgttccgt cgcttaaaac
     4201 cggttctgta agcacttttg accaagaaac gcgagaagca gaattatcaa ctccagattt
     4261 ttctatggtg ttgtactcgt aaaccttatc ggaagttcca cccaaagaat tattccatcc
     4321 cgtagggaca atcaaggatt gcccgtaaaa actcggatta tttgtagttt caatggttgt
     4381 agagtaaaat accacttcgc tggtagttgg ttgccacgga cgaccaaagt atccgggttt
     4441 ggagcgataa agtgaagctg tctccacaga aggaaccgcc gaagttatat tgcattcata
     4501 cattaaaaat ccgcgagcag aattttgttg tgcagctgta atgtaagcaa catccgagct
     4561 tgcttcactt gtattcattg ctaaatcggt ttggtagcaa gtcaatgtca tgcctccaaa
     4621 caagtaatcg gtggcaccca taagtacgcc cttataaaca gctattctgg cgccttcagc
     4681 tccataaaat gaatcctgac gaccaacaac tcgacatttg tttaaaattg ccctatcggc
     4741 acttgccgta aatgcaatag ctgcagctct ttctacaaag ttcttattct gtacatccgt
     4801 actcccgtaa gttgtaggac gggtaccctt tcctccggtt gaccattcct gaactacatc
     4861 ttccgattct tttcttgaaa tatattgatt aaaagaattt tcaaaaatta tattgtcagc
     4921 tctgaaaccg gaagctgaga ctaccactgt tgcattccag taagaaccgt tagttgtgcc
     4981 acttccggta tttgaataag ttgtataacc attttcctta ttgactctca gaacatctgc
     5041 attccatttt tggtcggtgc ccatgctgaa ataattgtat ccgtgtccat aatacgaagt
     5101 aattctcaca gcattttcat caatatccac acctttgttt aaaagagcaa tacttggtgt
     5161 tgtagaagca ttaaccaaag taacgtttgg aatatcaatc accaacattt cttcgtaatt
     5221 tccgggatct atcattattt ttacagtatc tgtattttca cgcgtcatac aacgaacggc
     5281 agctaatgcg tcattgatac tctgatattc cttatcggtt ccaaccgtta ttattggctt
     5341 gtaggttaca gattttacca ttttaatttc agttaaatag gtggtcgttc ctgtaacatt
     5401 agcaatggtc atgtaaccgg gagtagattc cgcataaaca aattcgtgcg attcaatgcg
     5461 gttggtactt ccgctgttgg tagaaatttc acttcctcca tccacagtga acttggcaga
     5521 ataatagtaa taaactatca ttttttcacc gggtgaaaca ggaactttag ccgttgcatt
     5581 acctgtgagt accaaatgtc ccttcgattt ttcattgtaa accgaaccgg taaacaacat
     5641 tcctttataa gccgttgtaa caccacttgt aatggtggca tcatcaaaag accaatccgt
     5701 aacgggaata aacgataaat cttttgaagt ttcagctcca ttaactttta agtttgacgt
     5761 aagtcccaat tgtacaggat attcgtccaa accactgcac gaaatggaat agactccatc
     5821 acgcaactga atattattca aatcggtgaa agaataaaca taacccgatt cattcaggtt
     5881 ggagaaagta acagaaagtt tggaaagttg cgtagcgtct aaaccgttaa tatttaacaa
     5941 aacagtataa accggcttag ctacaaatac aatatcggta ttaatatctg aagagatatt
     6001 tacattattg ttggagatat aataatcatt tacacctgta gcagaaatcg tataatcgca
     6061 atttgcttcc aactgaacag aataagtccc ggcagaagca ttcactacag gtttggggac
     6121 ataaattctg gtatcagaag gagtaaaaat taatcctaat ttcgccagtt tttcggaagg
     6181 caatccagta atattcccac ttaatgtgta aagtgtaact tttaagataa caggattata
     6241 agttgtattt ccactcaccg acaaagggtc ggaattggat ataatatagc cgttggcatt
     6301 actgagactt aaagaataag tgtatcctgc aggcagattt atggaatagt tgttagaagc
     6361 atcaggagct actgaccaaa cctttccggc agcattggta aaattaatcg tgtatcctga
     6421 aggtatatcc gaagcgttgg tcaaatcaaa cgtaccactg acggaaacat acgtggcagg
     6481 ttttctcaaa attctgtaat aaaatggttt atcaacggaa tcataaattt tatatacccc
     6541 cgctttcttt gctacaaatt tatatatttc cggtgatgaa gttaaagaaa cgatgtcttt
     6601 ctgagaatta tccgacgtat attcaaaagt cattttcccg gttccatttt gagatttggc
     6661 ataaatggta acctcatcgt cttcgtttaa atttaagctt aaatatcttc cggtagcagc
     6721 gctggcatta acataaagat atccggttaa agtttctgaa gttgaagcta ccgttaccgg
     6781 aacaccattt gaatcgtaac gcgtaagatt ggtattggat gttctcaagc ggtcgcttgt
     6841 agaagaagta ctggtccagc ttaaaatacc gacggtaaat gagctgggta aatttttccc
     6901 tgtggttccg ggtgttacgc ccggatacca actattaatt gttgcttcat ccaacatgtt
     6961 gttgtaaata gtaggatcga attgtgccgc accaaaatcc caaacatcta ttttggtttg
     7021 agcctttata attgaaaaac ctacaaagaa gaataatagc agaaagtaaa atattttttt
     7081 cattggtatt tttttaaaat ttattgataa ggtatgtctc tttatttatt cagcaataca
     7141 ctcaccatta taaaaggggc aaccgctttc gggtcattgt ccctgatggg ttcggaaatg
     7201 taatattcat aactgccgtc gcgataattt ttttcgccac ccaaaccggc tacagcacag
     7261 gcatttttca atgaaatagt tccatcagaa ttttcgacaa taaaagtttt tacaaattgc
     7321 ttgtaggctt ttttaccttt cttgagaaat tctttgggca aataaccttt ttgcgctcct
     7381 tttacccatg tataaataaa catggcagaa ccgctcgatt ccacataatt tcccttttca
     7441 ttgattttgt ctgtaacctg aaaccacatt cccgttttcg gatcacgata tttgtcgagc
     7501 gagtagcaca aatcagtcaa aactttaatt atttcgggtc gttttggatg attttcaggt
     7561 aagaaatcca gcacatccac tattgccatt gcataccagc ccatgcttct gctccagaaa
     7621 ttgggtgaag ttcctttcac agggtcagcc catttttgtt ggcgactctc atcccaaccg
     7681 tgataataca gcccggtggc aggatcataa caatgtttgg caataagcag aatttgaaaa
     7741 gctacttcgt caaataatgc cggttcatta aaccggtaag catattcagc caaaaaaggt
     7801 gaagccatat aaataccatc cagccacatt tgatgcggat aaatcttttt gtgccaaaaa
     7861 ccgccttcgc tggtgcgagg ctgcgtattt atctggctac gaagcagttc aattgctttt
     7921 ttgtattttt catctttagt tttttcatat aaagtgaaaa ggaatttgcc agagttaatt
     7981 cgatccaaat tgtattcttc aagtttgtac ccttttattt gtccattttc ctcaataatg
     8041 gtatcggcat aacttttggc atactgaaaa tatttttcat cgccggtctt ttcccagact
     8101 tttagaaaag cctgcaattc aagcccgttg caataatccc atttcagttt ttttgaaaaa
     8161 tcaagcatcc agctttcagg attacgtttc atttccgaat cagccattcg aacgtaatat
     8221 tttgattctt ttggagttaa actgaagcac gttaatgaaa atattaccag caaataaagt
     8281 tttctcattg ataatcggtc ttttaaatta aaaataaact tattttaaaa attcattttt
     8341 taaaaatttt ctcaaatcct ttgatttttt gagttgcaac ttcttttaca aacatttcgg
     8401 ctaccgttcg agctccagct tcattgaaat gagtattgtc atccaatcca ttgggataca
     8461 ggcgactaat acccggcgct attttatgga aatatttttt tgaaccttct acaccttcgt
     8521 gttgcaacca ttcagaagtg agtttttcta aatcgatcat tggaacccga taatctcgag
     8581 caacactgcg tataatttcg gggtaactgc cgtgtgttgg tacaaggcga cctgtgttgt
     8641 cgaaatttct tcgggctacc ggagtacaaa gaacaggagt cgcacctttc aatcttactt
     8701 cggcaacaaa tgccacaaaa ttcagcctga attgcgccgg attggtataa cgacccgttt
     8761 tttctacaga ctcgtcattg tgtccaaatt gaataatcac aaaatcacct ttttcggtat
     8821 tgttcaataa ctctgtccac aatccttctt ctataaacga tctggtactt ctgccatttc
     8881 tggcataatt tttcaccaaa gccttatcgt tgagcaattc gggcaataat tgtccccatc
     8941 cccgctcaag aaaaggttct tcataagatt ttcccgtcaa actgtcaaaa actgttttat
     9001 acagcacctt atcggccatt gtagaatcac ctgccatcaa aaccttgaca ggtttaggtg
     9061 ctgaagcaag aaaaaacaac atcaaaacac caagaatcgt aattgctgag aacctgaact
     9121 tcatttgatt ttaacatttg aaataattat attttcgttt tcctctcctt tcagttcaat
     9181 tgcattttga agtttttcgg gataggtgaa attttcggct ttaaaatttt ctatccgatt
     9241 taaacgcaga gcaggaccga ttgtcggttc aacataaatg tttttgaata ctatatcttt
     9301 ggaattattc aattctgctc cataagtagc tgtgataatt gcattttcaa cctgaatgtt
     9361 gctgattttc atttcaggca acccgttgaa aaacattgct ctgtgtgcgt tgcgcgaaac
     9421 tatgtttttt acgaaaatat ttctgaaaac gggtgtctct tctgtaacag gcatcaatac
     9481 agcatctttc tgattatcgt ttccttcttc catagcttct gtagcggact tgccaccata
     9541 atacaaatca aataaaaatg agtcggttaa aatatcgaac atgtaaattc cgtcaacaaa
     9601 aatattctca acaacgccac cacgtccacg tttacttttg aagcgaagtc ccacatccgt
     9661 tcctaaaaac tgacagcaac tgacagaaat atttcttact ccacccgaca tttcacttcc
     9721 aaccacaaaa ccaccatgag ctttgaaaac cttacagttg tgaacaatta aattttcggt
     9781 aggaacagct ctacgacggc cttcttcgtc tttgcccgat ttaatgcaaa tggcatcatc
     9841 tcctacatca aaataactat tgactatgat gacattttta caggattcca catcaattcc
     9901 atccccattt tgagcataat cgggatttcg gacgataatg ttatcaacaa tcacattttc
     9961 acacataaaa ggatgaatgt tccagcttgg agaattttca aataaaacac cctgcaagaa
    10021 aacatttttg cattgaataa aactcaccat aaccggacga agaaaatccc ttatttccat
    10081 ccattcttca tcagtcagtt ttgcacgcgg aacattcatg tcgcttatcg agtatccttt
    10141 gtatgatttt tccgatggaa accaataatt tccctctttt acaactccgc ccgaattgac
    10201 cacttttttc cattccgaag ctgtaacttt tgattttttc aaaggcctcc atgcttcccc
    10261 cgatccatta atcgaacctt ctccagtaat ggctacattt tcaatgttcc ttccggaaat
    10321 gggtgattgg cagcgtttgg ttgccaaacc ttcaaagtag gtatctacca aaggatataa
    10381 atcaaaatct tttgaaaaaa gaattaatgc tcctttttcc agatgaagat taatatttga
    10441 ttttaattca ataggtccag taaaccatac acctgcaggt acaattaatt tacctccacc
    10501 ttttcgggca agagcatcaa ttgcctgttt aaaagcttcg gtgtttaaag tttttccatc
    10561 accttttccg ccaaaatctt ctatcctcac cacattgttt ttaaaagtgg ggggctggat
    10621 ttttggcatt tcaaaaggta aatttttgaa aagatattcg taaggatttt ttgcatttaa
    10681 attggcagtg attaaaattg cacttgccag cattaaaaat aatgacttaa atttcatcac
    10741 ttttataatt aagagatcaa taaaatctat tttttaatga agcataaaaa cagcaataag
    10801 gctgtcttta tgcttctact cagctatgaa aaaaactacc aagaaatatt ggctccaatt
    10861 ccttgttcca acaaggtagc atttgttatg gttaaattac cgtttgcttc atcaacaaaa
    10921 ccgggatttt gctgcaggta agtgcccgaa ttatcagttt ttacattcag cgctgtacct
    10981 gtataaacaa gataattggg tgcattgaaa taattatttt tgttgaaaag tacaatattg
    11041 gtagctgtct ggttggtata atatcccact gaattagcca ctacattgtt ggtaaatgta
    11101 atttcattat tagcaaatcg tacgtataat aagcgacgag cagcatcatt atttacttta
    11161 ctaaaagtac aacggttgac tttaataatg gtcgactttc cagggaagct ggaagaggca
    11221 tcatcaagtc ggaaaaaatc acggtcggga gcacaattat aaaaagttga attcaataac
    11281 gttgcatttt caatacatcc agatcgaaca tcaatgcaat cggcactact ggtcaaaatg
    11341 ttggaaactt tgcaattttg aatggtaaat gatcttaccg aaacagcaat tctacttgca
    11401 cccgaaatca aagatttacc ataatttgta ataatacaat ccttgaataa aatatcacca
    11461 caagccacac tttgagtgag ctgcaccaca tgatcacgca tatcagcatc gctctgacca
    11521 ttcagaataa cgtcttttac agttaaagaa gcgttttctg cagttaatct aaattgcata
    11581 tgaagagtag gtttattgct cttcaaatat cctctgatac tgataccttt gtctatatta
    11641 attgtttgac cggcactttt taaaaattca ttacttccat cgtctgaggc aactaacaca
    11701 attctgctgc cggaaggtgc tgcctgaata gcagcaacca aatcttcatc aggatgaaca
    11761 acaatggtat ttgctgcatt taaatcaatt ttggtagtaa aagtagcact tccacgtact
    11821 ttgtcatctt tcatcaacct ggcagtatag tcggtatctt caatcaatcc gctnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctttgtc atctttcatc aacctggcag
    12001 tatagtcggt atcttcaatc aatccgctta gggtagcctc tccagctgct atttcctccg
    12061 ccgttaccac atgctctaaa gtactttggc cagccgttaa aataattttc gtagcagttt
    12121 ctccggcttt ccatgtcaat tttacactgt gtgctgaaac cgattcttcg gatacggcgt
    12181 tcataatttg ttcttcgccc gttttaaaat atacagcact ccatttcgat tctgcaattt
    12241 cacttcccac cgccttgata cgtactgaat attgcgttgc tcctatcaat ccactcttaa
    12301 tggtatccgg aatatcatca ttcgtaatat tagtaaaaat cattactgga cttccagcaa
    12361 atgtcaagct gtcattttca tatacttcaa ttacataact ttctgccttt ggattttttg
    12421 tccagttaag aaccacctgt gttttgttta tcacttttgc ttcaacacca acaggagaga
    12481 acaatcggtc tgtcaccaat tctgtaattt cttccggtaa ttcatcttta catgaactta
    12541 cagtgaagac aatcaataaa ctaaatagga taggaatatt tttaaatatc tttttcatgt
    12601 tgtagaattt aataaggtta gttaatagcc ataatcattt ttcagcaagc cgttactcga
    12661 atcgataaac acttgccata tgggccaaaa ttgacgagta tctggatctc tttgaaaaag
    12721 agaattaatt ttatcaacag ttaaggtgtt gacaccattg cttacaaacc aacccttgct
    12781 cgattcgtag tttaaagccg ctccttcggt atcggtatgt ccatgctcca atccgtatat
    12841 gataagcgat tcaccatcgc tggctgtttt atagtaaatt ttttcaggta ggtcggcata
    12901 ttcgccttga cgattagcta gtcgggtgag tttagctttt gcttcattca tttttgtaga
    12961 aagtaaattc cagcgaatca aatcggcctt cctcaacgat tcaccacaaa actcaagagc
    13021 acgttcgttg acaatagcat tgaaaaaagc tattttgctt gtggttacac ttgccatgta
    13081 agcgtcaact ttttcaggat tatttgggaa agcccgtttg cgaatttctc tcaaataagg
    13141 tgctgctgct tccggaccat caagttcgtt aattgcttca gcagccatta aataaatatc
    13201 ggcataacgc atgcattgcc agtttactcc gtcatcattg gttgaagtaa ccactctttt
    13261 catccattcg taacgcaatt ttccaaaaca ccacgaactc agtttgcgtg gcacttgttt
    13321 gccatttgtc cattcataag gcacacaagt aatatcacga cgaatatcat ctttatcaaa
    13381 atcgtaataa acataaggaa gcggtccatt ctgtccacct tgtgcttgtt gagtgtattt
    13441 atcggtagta gtatgtttta ctccaaatgt atataacacc cgaccacgtc catcggaaaa
    13501 tggtatttca taaagtgatt ctaatcctgc agtaacgttg tcttggcaaa ggtttctgaa
    13561 attttgttca aaagttccca atttacaggt cttgctttca atgacatcaa tacattcttg
    13621 tttaacgatt ggataaaggt tttcaaccga aagttcagga tcattacttc tgcgaatcgt
    13681 tccatcggga cgttgagaat atccagcagc agccagacaa attctggctc ttaaaccttt
    13741 tacaaaagcc ttgtttactc gttcagtggt tcgtgtaaca gccgtttcat taggccaagc
    13801 cacataattt tctgcttcgg ccaaatcggc aattatttgc ttgtaaatta catctctgtc
    13861 cgattttggc aaataaagcg tagaaggaga aatgggttca aaccgcgcag ggacatcacc
    13921 ccacgctttt atcaaatcat tgtaaatcat ggctcttaaa gtaagggctt cgcccagtat
    13981 ttgggcaagt tctggatttt tctccacatt tccataaagt cgcaaacctc taatacataa
    14041 attagcacgt tcaatagctt catacatttt agcccatgca ttgttttcca tattcatctg
    14101 agtattggta ggaagcggat cgtaaccggt cagttttgca cggtcatcac ttacattttc
    14161 agatgaattg taccactcga tatcggtgtt aatcccgtaa aaaggtatgt aacgaccacg
    14221 ataggaattg gtttctgcca aagattgatg aatgcccatc aaagctcctt ctgccaaaac
    14281 aggcgtagaa aaaattacct gttcgtccaa tgacgatttg gtgggagctt ctaaagtttc
    14341 ctcgcatgaa ctcattattc ccattaagaa aagagtgagt attgcagaaa taaatattgt
    14401 ctttttcatt ttatcaaatt atttacaagg ttaaaaattt aaatttaagc caaatactac
    14461 ctgtcggctt tttggatatg ccgaataatc aaccgatggt gtcaattcgg ttttacgtcg
    14521 agttgaaact tcaggatcga aaccggaata atttgtccag caatacaaat tataaccggt
    14581 aacataaaaa cgcaactgtt gaattttgat ttgtctgatt aaatgaaccg gtaaagtata
    14641 acctaaagta agtgtattta acctcaagaa ggaaccatct tcaacagccc aatcggaaaa
    14701 aacatatttt gacatatacg gagaccacat tgttgtattg gcattcatgg cttccaattc
    14761 tgccggattg ttgctaatgg ttccgtctga tttcaaattg gtccagcgtt ttccgtctgc
    14821 catgatggct aacatgtttc gatattgata tttactggta gaagtgtatt cgattttatt
    14881 ggcattgtac acatcattcc cataactcca gttaaaattt gcagaaaggt caaatccgta
    14941 caatcttcca ttaattgtaa atccaccggt atgtaaggga ttggcatttc ctattatcgt
    15001 ataatctttt gaatttacaa tgctgtcatt ggtaatatct ttcagtttca ttgctcccgg
    15061 gcgaagcgca tctgatccaa ttacggaaga acaatcaaca actccttcct tcaatatcca
    15121 ttttttcttt acagaatcgt atccttcaaa atcagatact tcatatcttc catcggaacg
    15181 gtagccatac atttgcccaa cagaacctcc tacagcaatc cagtaatcgt tacctatttc
    15241 agttgaagcc caaccactgg atgctccaaa gtcgctcatt attcccaaag aattgatttt
    15301 gtttttattg aatccaatgt ttccactgaa actcaaacca taattctttt tgtctattgc
    15361 tatccaattc agtgtaaatt caacgccctt attttgcgtt tctcccatat tacggtactg
    15421 agtatcataa cctgtaccag gaacggggaa attgatcaac aagtcggaag tggtattata
    15481 ataaccttca attgttccat ttaatttact tttcaagagg acaaagtcca atcccaagtt
    15541 tcgcgttacg gttgtctccc atttcaaatc gggatttgcc ataatttttg agggagacca
    15601 gtaagaagca acgttattga tccatgaagt agacttggaa tcgaaagatt gtgtcatctg
    15661 accggatggt atgttattgt ttcccgctgt tccatagctc aacctgagct ttaaatcatc
    15721 caaccaagat tgagtagatt ccataaaagg ttctgaagaa attctccatg ctaaggcagc
    15781 agaaggaaaa tatccccatt tattgccttt ggaaaactta ctcgaaccat cggctctaaa
    15841 tgttacactc atcagataac gacttaaata atcataattt gctcttccaa agaatgataa
    15901 aagtatatcg tcgggtgaat aataattctc gatattatag ggagttcctt gagaggttaa
    15961 cctaaaagca ttggtagaag taaagctttt gggatatccg tgaatagtat tagttaacgt
    16021 ttgcttacta ttaaaaatgt attcttctcc cagcaataca tccaaatgat gactagttcc
    16081 caataatttc ttaaagtcat aacttaccgt attggtattt ctgaaagtat tcaaaaatgt
    16141 attgttcaat tcggcagcgg gataaccctg attttcggag gaaggaatat ttttaatgta
    16201 ataagtggta gttccataaa aacgctggtc ttctgcacga taattctcgt atccaaactc
    16261 aactttcagc ttaaaattat caaatgcttt ccacgttata tctcctgcca tattaagatc
    16321 gttccgcttt tgttgacgat cgttatcgga aatagccacc aatggattat ataaattccc
    16381 tatttccgta tcatcactgg taccggcttc tgatgtcaga tcttttaaag gaaaaggagg
    16441 ataaatcatg gcatatttca aacgcgaatc ggcacttgaa acttcctttt gttcattcat
    16501 acctccacca ttgatttttg tgttgatgta tcgagtagaa aaattcattg aaacattttt
    16561 ggaaggattg gtagtgtact taaaattaat attgtcgcgc ttgaaacttg acatttgcat
    16621 aatggccttg tcatctatat gactgaaact aaaagcaaat ttgtttttgg aatcgcctcc
    16681 gttgatgctt aaattgtgat taaaagttgt tcctgtccta ccaaatacaa gatcttgcca
    16741 attgttggaa ggtacattgt tataaagatc aatatcctga taattgccaa aatacttttc
    16801 gtaagagctt gcatctccat cgtttttcaa caaagccagc tcatactgcc acttagcata
    16861 atctgatgga gacaaaacgt ccaatgtttt ggctattttt tttgctgcgg tatagaaatt
    16921 ataatttgct gttattttgc cttcttttcc cgatttggta gtgatgataa ctaccccatt
    16981 agcaccacga gaaccataaa ttgccgtaga tgaggcatct ttcaaaatgt caatagattc
    17041 aatatcataa ggagaaatgt catcaataga ttgtacagga aatccatcaa ctatcaacaa
    17101 aggtttgttg cttccggtaa tggaacctcc gccacgaaca cgtattctga tttccgcatc
    17161 gggagatcct tcagtagttg taacctgcac gccggccaat tttcctgaaa ttgcttctgc
    17221 tggagaagat acgggaatgt tcataatcgc atccgaacta accgaaacta cagaaccggt
    17281 aatgtctttt cttttcaccg tgccatatcc aatggcaaca acttcattca atcctatcgc
    17341 ttcttcggtc attaccacat taatgacact tcgattccct atttcaacag ttaacggttt
    17401 cattccaaca taggaaaact gcaacttggt ggcattagcc ggaacagtga tagtaaaatc
    17461 tccatttaaa ttggtaacag tacctgcaga aggagatccc gcaacaataa ctgatacccc
    17521 aattaatggc tctccatcag tagtttttac agttccttta atcgttcttt cattttgctg
    17581 aattaaattg ttctggtcag taaaaccata aacttgaaca aaaaaaccaa ccaatatgaa
    17641 aaacaaaatg gaaataaatt ttttttgagt tctcataaaa ttagatttaa ttaaaagtta
    17701 agtatttaaa attgatgtga ttcttatcct tctaaatgtt tcaatataaa aaataatctt
    17761 ttaattttat cgatgctaaa attaaaatgt gttcgcacac ggtttttatt tcaaaattat
    17821 ttttaatgtg ttgaaaaagc aatgaaatat atgattatac atttttttaa acttattact
    17881 cgtaataact atatgtctct gattaacaag taattatatt gtttttcaaa aataatgtgt
    17941 acgcacacga ttttatcaca acaaatgtaa tatataattc tctatttaat tcactttgaa
    18001 aaattaaata atgttaaata tatattgttg ttacattctt gtttttgtaa ttctctgatt
    18061 ttgtgatttt taaaataaaa ttttatttcc gaaaaattta tgttttgcac gtaagcagcg
    18121 aaaaataaaa aaagaacctc tgaatgctca tgctgataaa atattaccat cttaaattat
    18181 tgctaaataa tgtttgattt gcatggaaaa tttaataaca taaataaggt tgtattttgc
    18241 aattgacttt atagcccgaa ttggcaatta tatcatttgt aaacaaaata aggaataaaa
    18301 ctttgaaaat ttgcttttcc aactttttat ctatctttgc tcccataaaa tttggggaga
    18361 ttaatttcct taatattgtg aaaaggcatc ttttatttgt agatactatc acttttttaa
    18421 aagctaccaa tcttgtggtg aatttttaaa gaaaaatcaa atataatcac ttaaattaat
    18481 acgctatgag tatcaaattt cgcttgattc ttatgaattt ccttcaatat tttatttggg
    18541 gttcttggtt gatttctttg ggtagttatg caggaaatat tcttaacttt agcggagtcc
    18601 agattgggag tatctttgcc tcaatgggta ttgtttcact tttcatgcct ggattgcttg
    18661 gcattgttgc cgacagatgg attccggcac ataaaatgct gggtatttgt catttattag
    18721 gttcaatatt tcttattgca gcttcatttc aaacccaata tatggcattt tacagcttca
    18781 tgttcataag tgtaatgcta tacatgccaa ctatttcact aagcaacaca gtggcataca
    18841 atgctttgga aaaaaatcac tacgatattg tcaaagattt tcctccaatt agggtatggg
    18901 gaaccattgg gtttatttgt gccatgtgga cagttgactt cacggggttc aaattttcac
    18961 aagcgcaact ttatgttgct gctacagcgg ctttaatttt gggagtctat tcttttacat
    19021 taccaccctg cactgttaac cgaagcaacg aaaaaaaatc ggtaatagat gtattggggt
    19081 taagggcatt tactttattt aagcacaaaa aaatggccat cttttttata ttttccatgc
    19141 tattgggggc ttcacttcaa attaccaatg gttttggaga cttatttttg agaagtttct
    19201 ccgaaattcc tgaatatatg aattctttcg gagtaagaca ttctgttgtg ttactatcca
    19261 tttcacaaat atccgaaacc ctttttattc ttactatacc attctttttg aaaaaatttg
    19321 gaatcaaaaa tgttatgcta atgagtatgt ttgcttgggt atttagattt gcactttttg
    19381 ggatgggcaa tccaggagac agactttgga tgcttattct ttcaatgatt gtttatggca
    19441 tggcttttga ctttttcaat atttccggat ctctttttgt cgaacaatcg accgattctt
    19501 ccattcgggc atctgctcaa ggattattta tgatcatgac caacggactg ggcgcaactt
    19561 ttggatccta tctagccggc gctattgttg ataaatttgt tacccatgcc gaagttccaa
    19621 attggtcaac tgcctggtat atttttgccg gttatgcctt aatagtggga attttatttg
    19681 ctattctctt caaatacaaa cacaacacac aagaagttac cgaaaaattt cattgataaa
    19741 acactttttt cattgaaatg aaacaacgta aatccaagta aatcaattgt tttttcaaat
    19801 aatgaaagta ttcggttaaa ttcaccaaat tccgttttgt aacataaaaa ggggccgtct
    19861 caaaaaatta ggatagcccc ctctttattt ttcaagtttt ttatttattt gtttttgaaa
    19921 acaataccat tttcgtcagc atcaacaacc actacttggt tgctaacaat ctctccggca
    19981 attattttct tcgacaaatc gttcaaaata aatttttgta ttgctctctt taccggccgg
    20041 gctccaaact gtggatcgta gccattgtcg gcaataaact tcactgcacg atcggtagtt
    20101 tccaaccgga taccgttttg ttcgagcatt cggcgtacac catccaattg cagacgaaca
    20161 atatcttcta tctgcgaacg atcaagtggt ttgaacatga tcagctcatc gatccggttc
    20221 aaaaattccg gacgaatggt ttgtttcagc aattcaaaca cctcattttt cgttctttcg
    20281 atgatttcat catggttgta ttcgttaatt ttttcaaaat tttcacgaat caatgatgaa
    20341 ccaatgttgg acgtcatgat gataattgta tttttgaaat ttacagtacg tcccttgtta
    20401 tcggtcaaac gtccatcgtc cagtacctga agcaaaacat tgaagacgtc aggatgagct
    20461 ttttctattt cgtcgaaaag cacaacggaa taaggttttc ttcgtatggc ttcggtgagt
    20521 tgtccacctt cgtcgtaacc tacatatccc ggaggcgaac caataagtcg tgttaccgaa
    20581 aatttttcct gatactcgct catatcgatt cgtgtcatca tgttttcgtc gttgaacaga
    20641 tattcagcca atgctttggc caattccgtt ttaccgacac cagtggtacc aagaaagata
    20701 aacgatccga taggtctttt tggatcttgc aatccggctc tactgcgacg aattgcatcg
    20761 gcaacagcct gaatagcatc gtcctgtccc accactcttt tatgtaattc ttcttccaag
    20821 tgcaaaagtt tttccttttc actttgcaac atcttggtta cggggattcc ggtccagcgg
    20881 cttacaatct ctgcaatatc ttcgctgtcc acttcttcct taatcaacgg attatttccc
    20941 tgaatttttg ccagctcttc ctgatattga gctatggtgg actctaatgc ttttattttg
    21001 ccataacgaa tctcggccac tttgccataa ttgccttctc tttcggcctt ttcggcttca
    21061 aatttcaaat tttcaatgtc tatttttgcc tgttgaattt tgtcaatcag cgatttttca
    21121 cttgcccatt tggctcttaa ggcatcggcc tgctctttca gattggcaat ttcctgacta
    21181 agttgttcga gtttttttgt atcgttttca cgtttgatag cttcacgttc aatttccagt
    21241 tgcttaatgt ttctttcaat cacatccagt tcttccggca cagaatcaat ttccaaacgc
    21301 aatttggcgg cggcttcgtc catcaaatca atagccttgt cgggaagaaa acgttcggta
    21361 atataacgac tcgaaagttc aacggctgca attatggcgt catccttaat tctcacctta
    21421 tgatgatttt cgtagcgttc tttcaaccct cgcaaaatgg aaatagaact cggtatatcc
    21481 ggttcgtcca ccatgacgat ttggaatcga cgttccaatg ctttatccga ttcaaaatat
    21541 ttttgatatt ctgccaaagt tgttgcacca attgctcgca aatcgcctct ggcaagtgct
    21601 ggttttaaaa tgttggcagc atccattgct ccttcgcttt tccctgcgcc taccagcgta
    21661 tgaatttcgt caataaagag aataatttcg ccattggaat taaccacttc attcacaacc
    21721 gattttagac gttcttcaaa ttcaccttta tatttggcac ctgctatgac ggcacccata
    21781 tccaatgagt aaatctgttt cgattttaaa ttttccggaa catcgcctct cacaattctg
    21841 tgtgccaaac cttccacaat ggcagtttta cctgttccgg gctctcctat caaaatagga
    21901 ttatttttgg tacgacggct caatatttgc aacactcgac gaatttcttc atcccttcct
    21961 attacgggat ccaacttgcc gcttctggca cgttcgttca ggttaatggc atatttgttt
    22021 aaagaattgt atgtatcctc cgccgaagcc gaatttactt ttgagccctt tctcaattca
    22081 ttaatggctt cgcttaacga cttttcgtta acacctgcat cattcagtgc tttttttaca
    22141 ctgttgtttt ccacaaccaa tgccatcaga atagattcca atgccacaaa ttcatcacca
    22201 gcttttgaag aaatatcaat ggctttctgc aaaacagcat ttgtttctcg tcccagataa
    22261 ggttcgcctc cggaaacgcg tggataagtt tctatcattc ggtcgattac agaattcagc
    22321 atcgacaaat taacgcccat ttttccaaac aaatactgaa taacatcctc gcctttcaac
    22381 aaaacacctt tcagcaaatg aggcgtttcg acagcctgct gtcctttgct ctgaacaatg
    22441 tccacagctt cctgaatagc ttcctgcgac ttgatggtaa aattattgaa gttcataagt
    22501 tttatgtttt aaaaattttt taactatttc aattgttttt cctttttcgt tcaaaacact
    22561 taccaatcat caaaagagga aatattgacg tgatttttca ttcaattaac tgataaacaa
    22621 gaaaatattt cataattact gaaaatatgt cattaatgct gcgatagcaa attgcaatat
    22681 aaggatgaca atttccccgt tttcctttcc atttttaatc aaaataatta tttttgtagt
    22741 ttgaagttgt tcattcaaac tataaaaatt tttaaatgaa aaaaaaatat ggataataag
    22801 aaaagagttt acacatttgg taatgggcaa gctgaaggaa gcgccagtat gaggaattta
    22861 ttgggaggaa aaggtgcaaa tttagccgaa atgaatttgc tgggtattcc cgttccaccg
    22921 ggttttacca taaccaccga agtttgtacg gaatattacg ctttaggaaa ggataaagta
    22981 gtggaactac tcaaacccga agtggaaaaa gctatggaag gcgttgaaaa actaatgcgt
    23041 tccaaatttg gagacaaaaa aaatccattg ttggtttcgg tgcgctccgg agcaagagct
    23101 tccatgcccg gaatgatgga caccattttg aatcttggtt taaatgatga aattgtagaa
    23161 ggcttggctg aaaaaaccgg caatccgcgt tttgcatggg attcataccg ccgctttatt
    23221 caaatgtatg gtgatgttgt gttgggctta aaacctgcca acaaaaacga tatcgatcca
    23281 tttgaagcag ttatcgaaca attgaaggaa gaacgaggcg taaaactgga caccgagcta
    23341 acggttgatg acttgaaaga attggtcaaa agatttaagg ctttggttgc cgaacgaacg
    23401 ggcaaagaat ttccgctgtc ggtttacgac caactgtggg gagctatttg tgccgttttc
    23461 gattcatgga tgaatgaacg cgccatattt taccgaaaaa tggaaggaat acccagcgaa
    23521 tggggaactg ccgttaccgt tcaggctatg gtatttggaa atatgggaaa tacttctgct
    23581 acaggtgttt gtttcactcg tgatgcggct accggcgaag atgttttcaa tggagaatat
    23641 ttaatcaatg cgcagggaga agatgtagtg gccggtattc gtactccgct gcaaatcacc
    23701 aaacaaggtt ctcaacaatg ggcaaaactt acaggcattt cagaagaaga acgaaaagaa
    23761 aaatatcctt cgatggaaga agcgctgccg gaaatctata aagaactgaa tgaagtgcag
    23821 gaaaaactcg aaaaacatta caaagatatg caggacatgg aattcaccgt tcaggaaggc
    23881 aaactctggc tactgcaaac ccgtaatgcc aaacgtaccg gaacagcaat ggtgaaaatt
    23941 gccatcgata tgcttcatca aggaattatt gatgaacaaa ctgccttgtt gagagtcgac
    24001 cctttgcgtt tggacgaact gctccaccca atttttgaca aaaatgccgt aaaaaaagca
    24061 aaggtcattg caaaaggact ggctgcttca cccggagccg ccagcggaaa aatagttttc
    24121 aatgctgatg acgctgcaga atgggctgca cgcggagaaa aagttattat ggttcgtata
    24181 gaaacttcgc ctgaagattt agccggaatg gccgcagctc agggaattct tactgctcgt
    24241 ggcggaatga cttcacacgc cgcagttgta gctcgcggaa tgggaaaatg ttgcgtttcg
    24301 ggtgccggtt ctcttaaaat tgattatgct aaaaaaacaa tggaagccga tggcattgta
    24361 ctccgagaag gagattacat atccctaaat ggttcgacag gtgatgttta tcttggccaa
    24421 attcctacgc aggaagccga attggatgcc gattttgccg aactgatgac gcttgccgat
    24481 aaatattcga gcatgaaagt tcgcaccaat gccgatacgc cacacgatgc agccgtagct
    24541 cgaaaatttg gtgcagttgg cataggtttg tgccgtacgg aacacatgtt ttttgaagga
    24601 gaaaaaatca aggccatgcg tgaaatgatt ttggccgaaa atgaagaagg acgccgcaaa
    24661 gcactgggaa aaattctgcc ttatcagcaa gctgattttg aaggaatttt tgaagtgatg
    24721 gatggttttc cggttacggt gcgtctgctc gatcctccat tgcacgaatt tgtcccccat
    24781 gatgaaaaag gacagaagga aatggctgaa gctatgggac ttcccttaca aaaaatacaa
    24841 caacgcgtcg aagaacttcg cgaacaaaat cccatgttgg gacatcgtgg atgtcggttg
    24901 ggaaacactt atccagaaat taccgaaatg caaacacgcg ctattttggg agctgccttg
    24961 aaacttaaat ccaaaggaat caaaattata cctgaaatta tggtaccttt ggttgggata
    25021 aaaaaagaat ttctggaaca ggaacgcata atccgagaga ctgccgaaaa actttttgcc
    25081 gagaaaggtg atcgtatcga atacaaagtg ggcaccatga ttgaagtacc gcgtgcaaca
    25141 ctgattgccg atcagatagc tgaaaatgct gaatttttct cgtttggtac caatgacctc
    25201 acgcagatga ctttcggctt ctcacgagac gatattgcct cttttgttcc tgattacatc
    25261 gagaaaaaaa tcattgaaaa agatccgttc caagttctcg atcaggaagg tgtcggacag
    25321 cttattaaat taggagttga aaaaggacgt tctacacgca gtgatttgaa ggttggaatc
    25381 tgtggtgaac acggaggaga gccatcttcc attgaatttt gctatcgcgt gggaatgaat
    25441 tatgtcagtt gttctccttt ccgcgtgcca attgcacgct tggctgcagc tcaagcagct
    25501 ttgaaaaatt gaggaggcaa aatacgatta aagttaattc gaaaacacgc ctactcttat
    25561 tctttgcacc gtgcatgctt tatgcacggt ttttttgcgt ttttccatta ttattttctt
    25621 tgaattacat ggtttgtggt aaccttttta tcttttgggt gaaaaattta tcctttttac
    25681 acggattggg gaacaagaaa gcaattactt ttgcaacaat ttcaagcttt attcaataaa
    25741 atatcacacg gcattttaag caatcaagaa aaaacagaaa aaatcaagtc aataaatccg
    25801 actttttatc tgttgttttc attactgatg gtaatagggt tgtcttttgc agacagcctn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taatggaaca aagtgaacaa
    25981 atagttaata aaattgttga aggaatacaa gaaaaaaaag gaaagaaagt agtcattatt
    26041 aatttaaccg ggttgaaaaa tgcgccttgc agttattttg taatatgtga agggacaact
    26101 tcgatacagg tagatgccat tgggacttcg ataaaggata cggtaagaga aaaaatgaat
    26161 ataaaacctt atgctataga tggtttaagg aatagcgaat gggtggctat ggattacggg
    26221 tcggtaattg ttcatatttt tcggccacag attcgtaatt tttacgatat tgaacattta
    26281 tgggaagatg ccgaaataac agaaatttac gatgaatgat aaaagcaaaa caacaattta
    26341 aaaatattca aacaaaaaaa ttaaagagaa tttacaatgg aaaccaaatc tccacaaaat
    26401 agtcctcaaa aacccggcaa aggaccaaag tttaatatta catggcttta cgtcatcata
    26461 ataggtattc tgataggttc atatttgttt tcggatactg ctccagtaaa agaagtccca
    26521 ttttctgatt ttgaaaagta catgtataac aacaaaatgg aaaaaattga tgtgtactct
    26581 tctaaaaata ttgctgaagc acttatcata aaagattctt tgaagcaggt ttttggcgaa
    26641 aaatatgaaa tgtacgaaaa agaaccaaaa atcaggacaa gaattccttc tgcagaagag
    26701 ttttcaaaaa tatatcaaaa agccaaagaa caatatggcc ttacagctgt ggtcgattat
    26761 aaggaaagcc gcaattatgt agatattttc ctttatagta ttttaccttt tcttttgctg
    26821 atattgtttt ttgtttacat gaacaggcgc atggcaaatc agatgggcgg agggtcagga
    26881 ggaggcattt ttaatgtagg taaatccaag gcacaacttt tcgataaagg ggatgtggcc
    26941 aacaggatta ccttcaaaga tgtagccgga ttggaagaag ccaaacaaga agttgaagaa
    27001 attgtggaat ttcttaaaaa tccatccaaa tatacggaat tgggaggtaa aattccaaaa
    27061 ggagttttat tggttggtcc tccgggaaca ggaaaaactt tacttgcaaa agctgtggcc
    27121 ggagaggctg atgtgccttt tttctctatt tcaggatcgg aatttgtaga aatgttcgta
    27181 ggtgtaggag cttcacgtgt tcgagacctt ttccgacaag caaaagagaa agctccctgc
    27241 attattttta ttgatgaaat agacgcgata ggacgtgcaa gaggtaaaaa tccgaatttc
    27301 gggagcaatg atgaaagaga gaacacactc aatcaactac ttaccgaaat ggatggtttt
    27361 gcttcgaaca gtggggtcat tttgttggcg gcaactaacc gtgccgatat tcttgacaag
    27421 gcgttattgc gtccgggtcg gttcgacaga caaattcacg tagatcttcc ggatgtacat
    27481 ggaagaaaag aaatatttaa agttcattta cgtccaataa aaatcgacaa atcggtagat
    27541 atagattttt tagctaaaca gacgccgggg ttttcgggtg cagatattgc caatgtttgt
    27601 aatgaagctg ccttaattgc agccagaaaa aacaaatctt ttgtagaaaa acaggatttc
    27661 cttgatgccg ttgacagaat aataggaggt ttggaaaaac gaaccaaaat cactacaaaa
    27721 tttgaaaaaa aatccattgc ataccatgaa gccggtcatg caaccatcag ctggatgctc
    27781 gaacatgcaa gtcctttggt gaaagttacc attgtgcctc gtggagaagc tttgggggca
    27841 gcatggtatc ttcccgaaga acgtcagctt accgttagag cacatatgct tgacgaaatg
    27901 tgttctacat tgggtggaag agcagcagaa gaactatttt taaatcaaac ttctacagga
    27961 gccattaacg atttagaaag agtaaccaaa agagcttatg ccatggttac ttacttcgga
    28021 atgagcgaaa aattacccaa tttaagttat tacgattcaa gtggttcagc cgagtattcg
    28081 tttacaaaac cctatagcga aaaaacagcc gagttgattg atgaagaggt taaaaacatt
    28141 gtcagtcgag aatatgaacg agccaaggaa attttacggg aacacgccga aggacacaac
    28201 agactggccg aactattgat tgaaagagaa gttatttttt ccgaagattt ggaaagaatt
    28261 ttcggtaaac gaccttgggc atcacgcagt gatgaattgc tggctcaaaa cggggaaaat
    28321 gggaaatcat ccgaacggat ggaagacaat accgttgaaa atgaaaataa agaagaatga
    28381 tttgattaat gaagaagaac tatcttcgcg agaaatcatt ttaaaaaaga tttcggatat
    28441 tctttgcacc cgtacatcag aaactatttt ttcagaatat ttctcacatc agatttatag
    28501 ggaaattgct cccgatatgc ttacctgttt tatggaagaa ataaaggcag tatcgggaga
    28561 atgttttgtc catgacagtc catcgaactt attcgttgcc ctgaaaaaat ttttgttggg
    28621 aaagggattt tccaagatac attgtcgcaa tgaaaaaata acggaatatt tgaaaagttt
    28681 tgatattcct ttttcatctt cggaagccga ttttgaaata atggaagtgg gtattacgga
    28741 ctgtgaattt ctggttgccc gtaccggaag tgtaattgtt tcgtctttca attccggaag
    28801 aaaaatgaat atttttccac ctgtgcatat agttatagct catcgttcac aacttgttag
    28861 ttatctcgat gatgcttaca ggcaaattct tcagaaatac aaaaataatt tgccatcact
    28921 tatttctact ataaccggcc caagtcgtac ctcagatatc gaaaagactt tggttttagg
    28981 agcacacggg ccaaaacaat tgctggtctt cctgttagac aactaagacg ctgtttttct
    29041 ttataataaa aaatattgtg taagtttgta attttgaaaa cataaatcca tatgaacgaa
    29101 atgttaaaac gagcaattag cggctttatt tatgtaggat tgatagtaac aagcattctt
    29161 gtccatccac ttgcttttgc ttttgttttc ggcattctta cttattgcgc acttcatgag
    29221 ttttacaaaa tcacctatcg accttcagat atagaagttc ccatttctct tgcttcaatg
    29281 ggtggcttac ttttgtttgt ttctactttt ttgtttaaat ctggttttag ttcacccctc
    29341 attttttctt attatgcaat ttttgttata tggatgttta tcagggaatt gttcctcaaa
    29401 aaaagtaatc ctattcataa ctgggcatat tttatattgg gacaggtgat ggtggcactg
    29461 ccaatgtcgc tgttgatttt tattttattt gtagatggtt atcaacccat gcttttgttg
    29521 tccatattta ttttgacatg gctcaatgat accggggcct acctaattgg cgttaagttt
    29581 gggaggcatc gattttttga acatatatct cccaaaaaaa cgtgggaagg ttttttcgga
    29641 ggaatctttt ttagtttaac gggaggattt atattatctc tttttatcgc tgaactgagc
    29701 atctttcaat ggcttgccct atgctttata acagtattgg cagccacctt aggtgattta
    29761 accgaatcat tattgaaaag aactttaaac ataaaagact cgggaaacct tttacccggt
    29821 catggtgggt tactcgaccg tttagacagc atgttgttgg tttcaccctt tgttttgatt
    29881 ttttttcttt atatttttgt ttgaatttgc tgttctattg tcttttatga ataataagta
    29941 aatttattgc tgagaaaata aaaccatatt gaattgaaaa taaacagaat ctatggaaat
    30001 gaattttgat gacatacgtt gctacacaaa cgaggaggta catgaggtgt tggaaaaact
    30061 aaccaaagaa aaatcgttca tgaaagtggt tagcacagca taccctttgc tacccaaaga
    30121 aataattaaa gaacgattac ttcaatataa caatcaacag gaatttcaga aagattttat
    30181 ttttcctttt ttgcagtatc ttgaagaaaa cactaccaaa ggtattgaat tgttcggttt
    30241 ggaaaaaatc aatgtctcta aagcttattt atacttatcc aatcatcgcg atattgttat
    30301 ggattcaagt tttttttgca aaaaattatt ggaaaattca atagatacag tagaaatagc
    30361 tataggagat aatttgctta tttatccgtg gattgaagat tttgtgaagc tcaacaaaag
    30421 ttttatcgta aaacgtgggc tgaatacacg gcaaacgttg gaaagttcca aattactttc
    30481 tgcctatctg cttcatacta tttgtgaaaa aaatcagtcg atatggatgg ctcaaagaga
    30541 aggaagagca aaggattcgg acgactatac tcaggaaagt gtattgaaaa tgttgaattt
    30601 aagtgcaaaa gataataatt ttatccagca tattttacaa tttaatattt gtcccttatc
    30661 catttcatac gaatacgatc cttgtgatta cctgaaagcc aaagaatttc aattaaaaag
    30721 agacttcccc gactacaaaa aatcaccaac agacgatttg gaaaacatgg ttatcggcat
    30781 tatgggatat aaagggaagg tggtgtttcg tattacaggc tgcatcaacg atgaattaaa
    30841 aaaaatagaa caaaaaacat ccaacaggca cgaacaaatt tcccaaacag cacaattgat
    30901 agatctaaaa atacatgcca attatgaaat ttattcggtg aataaaattg catttgatac
    30961 gcttactggt gaaaatagat tcgctcatga atactccaat atggaaagaa ttgattttga
    31021 acagtatttg atgaagcaaa tagacaaaat tcaactggaa aataaagatg aaaaattttt
    31081 gaggcaaaaa ttgttggaaa tgtatgccaa tcccttaata aaccatttga agaccttgca
    31141 acattcttaa ccttccgtat aaaaaagaaa aaataaaccg gaacgatttt ttcaacacaa
    31201 aaaatcggac ttaccaaatt cactctccat agatacaatg tttaccattt gtaatcatca
    31261 gtcattatag caaatgggat agtgttttcc ataatttttt atggaagttg tcctgaaatt
    31321 gtttgaaaaa attatgcaat cgtttgcgtt agttgaaaaa atattctaca tttaccaccg
    31381 aaaaaaccaa aattaatttt ttaaaatgtt aaaatcatga gaaaactact actgttat
//
LOCUS       DAOTAX010000018        31048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1199_length_31048_cov_30.644081, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000018 DAOTAX010000000
VERSION     DAOTAX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 31048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_31048_cov_30.644081"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..471
                     /locus_tag="PLM04_03415"
     CDS             226..471
                     /locus_tag="PLM04_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HON02160.1"
                     /translation="MNIYVGNLSYKVRENDLQGVMEEYGQVSSCKIIKDRETGRSKGF
                     GFVEMSDDTAATKAIEELNGAEFDGRTMVVKEARPKN"
     gene            577..1455
                     /locus_tag="PLM04_03420"
     CDS             577..1455
                     /locus_tag="PLM04_03420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HON02161.1"
                     /translation="MSSLKGVFKNRKIFSQLLLLLFLSGLFFYIGLAFWMVISAGKRE
                     DAQAIRVLQLIQSIGLFLIPPFIFAYLVSDRKPTEYLSLSQKINLKDVMYVILIMFIA
                     IPFVNFLTSLNQQLVLPEVLAPVEEWMKQTEEEAALLTKQLLQVDNIGGLFANLILIA
                     LIPALGEELYFRGALQNVIGRGKGTHFAIWMTGFLFSAFHLQFYGLLPRWLLGVFFGY
                     ILVWSGNIWLPVIAHFTNNAIAVIFYYFSGQNNEVVNIDAVGTNDSWWMGVISLILTI
                     LLVFRLKSRIPKKQHI"
     gene            complement(1452..1847)
                     /locus_tag="PLM04_03425"
     CDS             complement(1452..1847)
                     /locus_tag="PLM04_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HON02162.1"
                     /translation="MWVLIIILISVGLVLMTVTYFSRKGKREEATVQVRENSECCGAH
                     ALCERENLLNMENEIEYYDDEELDALSGKSPTQFSDKEIEQLSEVFYSLQESDVAGWL
                     RSLQMRQIELPLFLREEALMIVGERRNAC"
     gene            2181..3578
                     /gene="nhaA"
                     /locus_tag="PLM04_03430"
     CDS             2181..3578
                     /gene="nhaA"
                     /locus_tag="PLM04_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HON02163.1"
                     /translation="MIDHHSKRARIYIFLRNFKWETNPGIVMMVVAIATLVIANSPLK
                     EWYQSFWNIEFLLGTNSFNLFSHHGTPLTLLSFINDCLMAVFFFSIGLEIKREVLIGE
                     LSSFRQSILPMIAALGGMIVPVLCFFVTGRIQNLSPDEMRGMFIPMSTDIAFSLAVVS
                     LLGKKIPVSLKVFLLALAIIDDIGGIIVIALFYSDFNAESVIYLLYAAFFVIVLALGG
                     KLRINGKLFYAFFGIIVWYLFLQSGIHPSIAGVVVAFTVPAHPRINIRTFIRQLRRDI
                     DLLESVSPKPTKEEIILSNTQIKYLSHIENAADQVISPLQDFEDNLYVLVNFIILPLF
                     AFANAGVVINLSDFYLSGIALAIIIGLVLGKTLGIFSFTWLTIKSGLSRMPNGMNWHS
                     LFGLSLVGGIGFTVALFLTALSYPVGSEILNQAKLGIIAGSLISGFLGYYYLKFSINR
                     GEKNNYRLKKNSQAS"
     gene            3688..5205
                     /gene="guaA"
                     /locus_tag="PLM04_03435"
     CDS             3688..5205
                     /gene="guaA"
                     /locus_tag="PLM04_03435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HON02164.1"
                     /translation="MEKIIILDFGSQTTQLIGRRLRELNEFCEILPYNKFPEQTKDIK
                     GIILSGSPFSVYDPKALQIDLNKIRGKFPILGICYGAQYIVHSAGGKVQPGNTREYGR
                     ASLTYVDATDPLMSNIPVGSQIWMSHSDSITEVPHNFIQIGSTQDVQIAAFRIEGEKT
                     WGVQFHPEVFHTTEGLLLLENFLNICDCRRNWTPASFIETTIQQLKEQLKDEKVVLGL
                     SGGVDSSVTAVLLNRAIGKNLTCIFVDHGLLRKDEFREVLTNYEHLGLNVIGVDAKER
                     FYAVLRGIADPEKKRKIIGAEFINVFNEEAKKIENVKWLAQGTIYPDVIESYSISGNT
                     IKSHHNVGGLPEKMHLKLCEPLRLLFKDEVRSVGIELGMTPQLIKRHPFPGPGLAVRI
                     LGEVTPQKVAILQEADYIFIQELRKWDLYDKVWQAGCVLLPIQAVGVMGDERTYENVV
                     ALRAVTSTDAMTADWAKLPYDFLAKVSNEIINKVKGINRVVYDISSKPPATIEWE"
     gene            5330..6118
                     /locus_tag="PLM04_03440"
     CDS             5330..6118
                     /locus_tag="PLM04_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HON02165.1"
                     /translation="MIIQTSMIDTHAHIYSEEFDADREKVIERALKRGIEHIILPNID
                     LQSLQPMLQLEASHPDICHAAIGLHPVSVKENFAETLNFLKFKLQHHNYIAIGEIGID
                     LYWDKTYQNEQIEAFCQQIEWALEWELPVIIHVRDSFKETMQALEPYRNKKLRGVFHS
                     FTGTIEEANQIITFGGFKIGINGILTFKNSELSKTVSQIETEYLVLETDAPYLSPVPF
                     RGKRNEPAYLSYICEQLALILGKTSEEVEFITTQNAKKLFKLQP"
     gene            6213..6302
                     /locus_tag="PLM04_03445"
     tRNA            6213..6302
                     /locus_tag="PLM04_03445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6247..6249,aa:Ser,seq:gga)
     gene            6382..7191
                     /locus_tag="PLM04_03450"
     CDS             6382..7191
                     /locus_tag="PLM04_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HON02166.1"
                     /translation="MKKVFAILSIVAIFSFGSALHASAQDTSAPQTEQTAGNDTAVAA
                     AQAMEQIEAPAPAQGGGMHKALKQKFIEGSPAWMAPIALCLIIGLALCIERIIYLNLA
                     STNTKKLLDDIDQAWAKGGVQAAMEVCRNTRGPVASIFYQGLSRYDEGIDVVEKAVAS
                     YGGVQLGLLETNLTWISLFIAISPSLGFLGTVIGMIEAFDRIQQVGDISATVVAGGIK
                     VALLTTVFGLIVAMVLQVFYNYILTIIENMTNDMEDSSISLLDIIVKHSKK"
     gene            7215..7700
                     /locus_tag="PLM04_03455"
     CDS             7215..7700
                     /locus_tag="PLM04_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02167.1"
                     /translation="MSKFMQKIPIISAIFLVSVSVILVLLIYVGGTSGSINNSAGEAM
                     SVPRFTDALIYWSYFLVGLAVVITLGFVVKNFFQSSKQSSSSIFKSLLPIIGFILLFV
                     VSWLLGSDQELKIIGYEGAENVGFWSRFTDMMIFPIYVMAIGIILTIIGTSIYSSLRK
                     D"
     gene            7752..8354
                     /locus_tag="PLM04_03460"
     CDS             7752..8354
                     /locus_tag="PLM04_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HON02168.1"
                     /translation="MSKKKRKVGQINASSMADISFLLLIFFLVTTSMNVGTGIARRLP
                     PPLPPDQKPPDMDIKKRNIFIVRINSQNQIMAQGEILDIKQLRYKTKEFIKNENNDPN
                     LPELITENIPLLGEVTYTKNHVISLQNDVGTQYQAYIDVQNELIAAYNELRDEFAMQR
                     FGKKYNELSQELQEAVQKIYPQKISEAEPKNYGGVKSGKN"
     gene            8341..8811
                     /locus_tag="PLM04_03465"
     CDS             8341..8811
                     /locus_tag="PLM04_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HON02169.1"
                     /translation="MARIRKSEKREMPALSTSSLPDIIFMLLFFFMAVTTMKEVTYKV
                     RIDPPKATEIQKLENKSLIRYIYVGKPLEQYQRIYGSETRIQLSDMFADISEIEEYII
                     NERSAMKEEDQNLMSVCIRADKDTHMGIITDIKQALRRAYALKIIYAANQRTQY"
     gene            complement(9039..10082)
                     /locus_tag="PLM04_03470"
     CDS             complement(9039..10082)
                     /locus_tag="PLM04_03470"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HON02170.1"
                     /translation="MKKIIVSFLMASVISSVLCQSSLTLNKNESVLIYSLPMTELCIK
                     VDVEKITRKPGVFYRYSERYLATSDIITEEKTVYSLKDIQVTSNAVPDPSKTYAVRVD
                     KNSPFVSISVDQNGLLCGINVPPPTSLPELSVLAPTETKENLSNNVILPLGEEYMLVG
                     SVAKLAEGAAKQIYRIRESRLNLLTGDVDHFPSDGTSLSAILNELDNKEKELTELFIG
                     STTKEIQKHTLYLRPQQALNNEVLFRFSVLKGLVPADDLSGVPYYISVIPTKSPTILD
                     NPKQKAPKEYINFVIPSATLVTVSDGKSTFFSQQFLFPQFGIVIPLSSSMIKGNDKIV
                     IIDPATGRLLSIF"
     gene            10529..11296
                     /locus_tag="PLM04_03475"
     CDS             10529..11296
                     /locus_tag="PLM04_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON02171.1"
                     /translation="MGKIISLANQKGGVGKTTTAINLAASLASLGKKVLLVDADPQAN
                     ASSGLGIEIQSLKYTIYECLIDGVSPQQAIHGTEIENLSVLPSHIDLVGAEIEMLNME
                     EREKVMQHLLRPLKPDFDFILIDCSPSLGLITVNALTASDSVIIPVQCEYFALEGISK
                     LLNTIKIIKSKLNPSLDIEGFLLTMYDNRLRLANQVYAEVKKHFQNMVFENVIYRNVR
                     LSEAPSYGKPVMLYDPESKGSSNYLDLAKELIDRQKS"
     gene            11344..12225
                     /locus_tag="PLM04_03480"
     CDS             11344..12225
                     /locus_tag="PLM04_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HON02172.1"
                     /translation="MVKKSALGRGLGALIDTDDIYTSGSSSISEVDVDLIFPNPNQPR
                     SHFDEESLAELASSIRELGVISPITLRKNDDGTYLIIAGERRYRASKMLGLKSIPAYV
                     KTAADEQVVEMALIENIQREDLNAIEVALAFYRLLEEYHLTQEQLSERVGKKRATIAN
                     YLRLLRLPAEIQVGIKDKKIDMGHARAILGLEDPAVQLQLYEAILENFYSVRKVEEMV
                     RLYAETGSFEEKHVRVSKPNPIRKLKEYEELKNQLSKTFGTKVQFSCNEQGKGKISIP
                     FSNDEELSRIMGLLDKI"
     gene            12238..12915
                     /locus_tag="PLM04_03485"
     CDS             12238..12915
                     /locus_tag="PLM04_03485"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HON02173.1"
                     /translation="MKRFFLITAEIWFISLFGIVLNAQNVNEKREIVQDSINRLPEKS
                     LQKESQVSIDTVQKPVYMPNPSKIVWMGAVVPGWGQILNRKYWKLPIVYGGLLGCYYA
                     YSWNASRYNSYHTAYLDIIDSDPTTNSFLDILPAGYTLESIGGTGRYTTILKSGQDQF
                     RRQRDLSVIVSVAFYALTLVDAFVDAQLYGFNISPDLSLEIKPIYLKDNITTSGGLGM
                     QCRFKLQ"
     gene            12941..14218
                     /locus_tag="PLM04_03490"
     CDS             12941..14218
                     /locus_tag="PLM04_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HON02174.1"
                     /translation="MNLKFSIIVVSFICFSINSVISQAAKDFPEDTLSSKIDSIIGIP
                     DAYGYALDHLLHLWALQRMDTTECNSGEPPAPVSDSLYKYRLSQIPCLIELPYNSIVR
                     SFIELYTVRKRTQTEYMLGISNYYFPVFEQALDANKLPLELKFLPIIESALHPTAISR
                     AGAAGLWQFMIPTGRMYGLEINTLVDERLDPRKASYAAARFLKDLYAIYGDWNLVIAA
                     YNCGPGNVNKAIRRAGGKTDYWAIYPYLPLETRGYVPIYIAATYTFHYAEEHNLCPAV
                     VNIPALIDTFMINQRIHLEQISSVLNIPIEELRWLNPQYKKDIIPGDLKPYVLTLPVN
                     YSSIFMERLHEIASYKADELINNRRNEIEAFRSTSSSNLGTSKNVTYHKVKKGETLSG
                     IAAKYGVSVSNLKKWNNIKGSKITIGQRLKIIK"
     gene            14227..14547
                     /locus_tag="PLM04_03495"
     CDS             14227..14547
                     /locus_tag="PLM04_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HON02175.1"
                     /translation="MEKVYYSISEVAEMLKISPSNLRFWEKEFSQLKPKRSNKGTRTY
                     TKEDIEIIKMIHLLVNEQKLTLQGAKRKLSLKKETVSKQQELVERLQNVRKELLMISK
                     ALDE"
     gene            14571..15641
                     /locus_tag="PLM04_03500"
     CDS             14571..15641
                     /locus_tag="PLM04_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HON02176.1"
                     /translation="MKKTNFCLGLIAALILMSASVSAEKKPKNVILMIGDGMGLAQMY
                     SAMTANNNQLQLERCTYTGFSKTYSSDHYTTDSGAGGTAIACGVKTKNGMIGMTPDSV
                     KIESILELSERNQLSTGIVVTCAVTHATPASFIAHQISRNMYEEIAGDFLQTDVDVFI
                     GGGLKYFNNRKDQINVVEKLKKNNYQVAYSLDEAVKVKSGKLAGLFYENHCPPMPERG
                     DMLPKSVMAAIDILDNNKKGFFLMVEGSQIDWAAHDNDKKQIVKEMLDFDETIGKVLD
                     YAQKDGNTLVIITADHETGGMTILNGKFGSDEMDAHFSTKNHSGVPVPIYAFGPGAEQ
                     FTGIMENTAFKGKIEKLLDLKK"
     gene            15738..16845
                     /gene="prfB"
                     /locus_tag="PLM04_03505"
     CDS             join(15738..15800,15802..16845)
                     /gene="prfB"
                     /locus_tag="PLM04_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HON02177.1"
                     /translation="MITIEQLKDVTERTEALRRYLDVDNRQIQIEEEELRTQEPNFWD
                     DPKKAEAQMRKLKELKNWVTGYQEVKTAADELELAYDFFREGVIEEQELDETYRKALQ
                     LIEQLEMNNMLSHEEDQLGAVLKIVAGAGGTEAQDWAEMLMRMYQRWAERKGYKWEIT
                     NFQPGDEAGIKSVTIQIEGERAYGYLKSENGVHRLVRVSPFNAQGKRMTSFTSVFVVP
                     LIDDSIEIEINPANLSWDTFRSSGAGGQNVNKVETGVRLHYTFKHPVTGEQSEIVIEN
                     TESRSQFDNKDNALRLLKSQLYELELQKRRAEQEKVESTKKKIEWGSQIRSYVFDDRR
                     VKDHRTNYQTSNVQAVMDGDIDEFIKAYLMEFPD"
     gene            17263..18924
                     /locus_tag="PLM04_03510"
     CDS             17263..18924
                     /locus_tag="PLM04_03510"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HON02178.1"
                     /translation="MKETKYIFVTGGVVSSLGKGIIAASLGKLLQSRGFKVTNQKLDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHRANNITTGRIYQNVIE
                     KERRGDYLGKTVQIIPHITDEIKRNIKLLGSKGDYDFVITEIGGTVGDIESLPYIESV
                     RQLRWELGKNCLIVHLTYVPYISAAKELKTKPTQHSVKALQEQGVQPDILVLRTEHNI
                     SSDLRKKVALFCNVEPNSVMQSVDVSTIYRVPLMMYDEKLDEVVLRKMGFDLLKIPQA
                     NLSKWIQFVEKLENAKEEVHIALVGKYVQLPDAYKSIIESLLQACAYNDRKLKLDLIL
                     SDKLTEENIEKKLEGVDGVIIAPGFGQRGIEGKLVTIKYTREKNIPTFGICMGMQCMV
                     IEFARNVLGYADANSTEIDPTTTHNVVDIMEEQKNILNLGGSMRLGAFPCKLKKGTKV
                     FKIYGKENISERHRHRFEFNNYYKEEFDKAGMICSGINASSNLVEIVEVPSHRWFIGV
                     QFHPEYSSTVINPHPLFISFIKAAIEYRNESKEKTQNSKFKTAIK"
     gene            18981..20885
                     /gene="yidC"
                     /locus_tag="PLM04_03515"
     CDS             18981..20885
                     /gene="yidC"
                     /locus_tag="PLM04_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HON02179.1"
                     /translation="MDKNTITGFLLITVIIVVFALLNRPNQEMIEKQRRYNDSIAQIE
                     KAKAESETAIQEVNSLLSDSIKTTLDSSAVIRDAYGDFSVAALGKEEFITLENSVLKL
                     TFTNKGARIYAAELKDYRRHDSLPLILFQGSEANMGFTLITNNNRILNTANLFFEVLL
                     PIEKDTLGNQILVFRLKTTGEGYMDVVYTLPPDNYMFSFSIKANKMSNVMPAGTNFLE
                     MQWTTRLRQQEKGRQFESRYSNIQYKYLSDDVEKLSESKNDSKQITNKLKWIAFKDQF
                     FSNVLIAEDAFTSSFLESKVEPEDSPYLKSYRADMTIPFDPTGKTSTNFRMYLGPNKF
                     NILASYDKGVAKEEKLQLHKLIPLGWGIFGWINRYMIIPMFNFFSKYIGNYGIIILLM
                     TIIIKIIIFPMTYKSYTSSAKMRVLKPEIDEIYNKIPPEKAMERQKAVMDLYSKVGVS
                     PMSGCLPALLQIPILFAMFSFFPASIELRQESFLWAKDLSTYDSIISWNAYIPIISKY
                     FGNHLSLFCLLMTISSIISTKLTMTTTPTTNQPGGNLMKWMMYLMPVMFMFIFNNYSA
                     ALSYYYLLSTLITIIQTFIIRSMVDEKKLLAQLHAKRNQKQPVKKKSSWIERMEKMQR
                     EQQKALKNKR"
     gene            20967..21977
                     /locus_tag="PLM04_03520"
     CDS             20967..21977
                     /locus_tag="PLM04_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02180.1"
                     /translation="MTKKIKFFILFFLIIFLFSCQNHNRFRINTKKNEVEVRIRRFDK
                     DLINVDTTNLKAEIRKIYKDYPVFFPVFINEILNVAPSDTDSVAKLIGDFLCDAAFQK
                     VNKEVLKTFADVHDIEKQLSIAFTYMHHYFPEIRLPEIYFFVSGFNRSIIIYPSLIAV
                     GADLYLGADYPLYKSFTYEYLTFNMRPESIAPDVVSTLLFSYFPMESENNRLLDNMLY
                     NGKILFLLSVFMPDVEPSVLMGYTREQWQWCKRFEKEIWAKIIEDKALFSSDMRLINS
                     YLDEAPFTAPVSQDSPGRVGTWIGWQIVNSYMKKNNNVSLRQLANENNYQLLLEKSDY
                     RP"
     gene            complement(22044..22211)
                     /locus_tag="PLM04_03525"
     CDS             complement(22044..22211)
                     /locus_tag="PLM04_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HON02181.1"
                     /translation="MAYVITEDCIACGSCITECPVEAISEGDIYVIDPDVCTDCGVCA
                     DVCPSEAIIPA"
     gene            complement(22589..24196)
                     /locus_tag="PLM04_03530"
     CDS             complement(22589..24196)
                     /locus_tag="PLM04_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON02182.1"
                     /translation="MDRNKKNYLAPLIVFLAVIFGLILGNLLAQRAFFSSSPNIFFGF
                     GNASKVDELLQIINTNYVDTLNLDEMTENVMQDIIAKLDPHSVYIPAEDISLANSELE
                     GSFSGIGVQFNIQNDTVMIISVISGGPSETAGLMPGDRIVEVNDTTFVGKSINNEKVM
                     KKLRGPEGTKVKLGIKRNQTKEVLYYEITRGKIPVNSVDIAYIVQPSVGFIKVSKFAE
                     TTYGEFLNAIAKLKNEGADKFIIDLRGNSGGFMDQSINMINEFLPANRLIVYAQGKAY
                     KRFEAKSDGKGSCTREPLVVLVDEFSASASEIFAGAIQDNDRGTLVGRRTFGKGLVQQ
                     QFNLSDGSAVRLTVARYYTPSGRSIQKPYKLGNSEEYEKEFINRLLHEINDSIFHPDS
                     LRYKTVGGRIVYGGGGIMPDVYVPLDTSEFTPYFNKVVNYGYVYQFAFQYSDKNREKL
                     KKIKNWHEMDTYLNSQSLLDQFVKFVSEKGIQANKKEINISKNLLLTQIKGYISRNIL
                     GDEGFYPLLYRSDKTVLKALDVINSKQ"
     gene            24653..25030
                     /gene="gcvH"
                     /locus_tag="PLM04_03535"
     CDS             24653..25030
                     /gene="gcvH"
                     /locus_tag="PLM04_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HON02183.1"
                     /translation="MKVPENLKYTTEHEWIRVQGNTAWVGITDFAQHELGEIVFVEVE
                     TLGENIHAGEVFGTVEAVKTVSELFLPISGEILEFNKELENKPELLNDDPYGEGWIVK
                     IRVTDPSELEKLLSAEQYAKLIA"
     gene            25062..25568
                     /gene="purE"
                     /locus_tag="PLM04_03540"
     CDS             25062..25568
                     /gene="purE"
                     /locus_tag="PLM04_03540"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HON02184.1"
                     /translation="MKARASIIMGSTSDLKIMEKAAKLLDEFEIPFEIHALSAHRTPK
                     EVEDFALHAEKRGIEIIIAGAGMAAHLPGVIASMTTLPVIGVPIEASLNGMDALFSIV
                     QMPPGIPVATVGINAAQNAAILAVQILALKDEKINSKLKLYKENMKQKIVEANNQLKE
                     IKYTYKTN"
     gene            26147..28366
                     /locus_tag="PLM04_03545"
     CDS             26147..28366
                     /locus_tag="PLM04_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HON02185.1"
                     /translation="MTTYPSQSVFSLYRRMVTSEKNMFSFQERKAIKQILKEYYLLHP
                     QQLTLPGHSIEDKLYIIQILLNEIGLGKASVLSVLFMEPIKNNYLTVSEIEQKFKTPL
                     DSIIGGMQRVEELYERSFSLETENFRKLLLTFAQDVRVILMMIAERLYFMRILDKFPA
                     DMQQSMARECSFLYAPLAHRMGLYSIKSEMEDLSLKYTNREIYREIAQKLNETKRSRD
                     KYIEEFITPLKEKLGKEIKYPFEIKGRTKSIYSIYSKMLKQNTPFENIYDLFAIRIIL
                     DVPLEKEKMICWQVYSIVTDMYQPNPKRLRDWLSIPKSNGYESLHITVLGPQNKWVEI
                     QIRTTRMDEIAEKGLAAHWKYKGIKGESGMDEWLKNIRDILENPDLNAVDFMDEFKLN
                     LYDQEVFVFTPAGDLHKLPKGATVLDFAYLIHSNLGSKCIGAKINKERNVPIKYVLKN
                     GDQVEILTSPSQKPNPSWLNIVVTSKAKSKIRQALKDIEFKDAEIGREILLRRFKNWK
                     IELEEGKLSRLAKKKGYKTVSDFYQAIAHEKIDMLLIRDAYLDLDRHETGAEQAEPRS
                     AETFSKETPAEELSSKEDVLIIGQNLKNVDYTLAKCCQPIYGDDIFGFVSVGGGIKIH
                     RTTCPNAPQLISKYGYRIIKAQWAGRSGGTQYPITLYIVGKDDIGIVTNITSLISKEK
                     NILLRSISVDSNAGVFQGNLTIMVNDANDLDSIIKKIKQVKGVLNISRTKSLTQNNQ"
     gene            28380..28979
                     /locus_tag="PLM04_03550"
     CDS             28380..28979
                     /locus_tag="PLM04_03550"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HON02186.1"
                     /translation="MFSGIIEEAAPVVALKKEAGNLHITMTCSFVNELSVDQSVSHNG
                     VCLTVVSKTPDTYTVTAIRETLEKSNLGLLQEGSKVNLERSLLLTERLDGHIVQGHVD
                     TTAVCTDISEADGSWYFTFQYDVDRELAKRGYVTVDKGSVAVNGVSLTVCNPTENTFQ
                     VAIIPYTYEHTNFHQIVVGTTVNIEFDIIGKYISRLMKL"
     gene            29066..30223
                     /locus_tag="PLM04_03555"
     CDS             29066..30223
                     /locus_tag="PLM04_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02187.1"
                     /translation="MENFRKQIKISFFNPIIHFLPLLLFLVTAMYAGRTVANYVSIFV
                     LLVSAVYVFIAYNQIFIWFFLSCVIFLAVNIVGSIIRLPIIFLSFRPLLDEALTLVFL
                     GGMILLQKPIKKGIMALIPKTLPMNNNLDEYFGFIRTIFLIILGYVLATGFFYAFPTQ
                     RETAYLQLVQAIYVGILFFVLVFESLRVQIIRARLLKEEWLPIVNSQGKIIGSVQHQA
                     SLGEKRKFMHPIIRVHIIQDGMILLRKLPADDLSYPLHWDTAISNHIRMGETVEDCIK
                     RTAQELYGLTELKSFFLSNYITHTDFEQHYAFLFIACKMNKIDLQSEKVKQAKWWTLQ
                     QIEENLQEGIFTENFLIEFDLLKKSGVLENNICDCACKLKETIFKAPHLFR"
     gene            30245..30820
                     /locus_tag="PLM04_03560"
     CDS             30245..30820
                     /locus_tag="PLM04_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02188.1"
                     /translation="MMRLYFILLLIAFVSCGGHSNSEFKLQNSFQIKLQDSICINAKD
                     CFYFSTDSNLYRIFMYFSNAELKEKKIIDTVDFSPYKSKIHSFQSENNESYVVLWETE
                     YEFYPLIYVYFITAGKIVKIGELLISLPCQSCESFEYPIKDIRMLQKGMNIEISFLKD
                     VNYKPSNGNEWHLYKAGMLKCIFNAETNKLK"
BASE COUNT        10010 a         5340 c         5946 g         9752 t
ORIGIN      
        1 gaaaaaatta aaatttttta tatactgaaa taatttttct attttttttt gttatttgaa
       61 aaaaaagcat ttcctttgta tccgattaca agaagtgtat ctccctcgat cggttatttt
      121 caactttcca tttctctgga cggcaatctt taacctcgta tgtttgtttt actcctcttt
      181 aatcgcaaat tatattaatt tctaattttt aattttttta tttttatgaa catttatgtt
      241 ggtaacctga gctacaaagt tcgggaaaat gatcttcaag gggttatgga agaatacggt
      301 caggtatcat cctgcaaaat cattaaagac cgtgaaaccg gaaggtcaaa aggattcgga
      361 tttgttgaaa tgagtgatga cactgcagca acaaaagcaa ttgaagaatt gaatggagcc
      421 gaattcgatg gaagaacaat ggtagttaaa gaagcacgtc caaaaaatta agcttttttt
      481 aaaaatcgaa atggagaact tctattgtcg caagacaatg gaagtttttt gtttttaact
      541 gttttcacga atgattaatg atatatcgac agaagtatga gctcattaaa aggtgttttc
      601 aaaaatcgta aaattttttc tcaattattg ctgttgttgt ttttaagtgg cttgtttttt
      661 tacattggtt tagctttttg gatggttata tcggcaggaa aaagagaaga cgcccaagcc
      721 atccgagtat tacaactgat acagtcaatc ggattgtttt tgattccacc atttattttt
      781 gcatatttag tgagtgatcg taaaccgaca gaatatctca gtttatctca aaaaatcaac
      841 ttaaaggatg tgatgtatgt gatactaata atgtttattg ctattccatt tgtcaatttt
      901 cttacatctc ttaatcagca actggtttta ccggaggtac ttgcacctgt ggaagaatgg
      961 atgaaacaaa cagaagaaga agctgcattg cttaccaaac aacttttaca ggtcgacaac
     1021 ataggtggat tgtttgcaaa tctaatatta attgccttaa ttccagcatt gggagaagaa
     1081 ttatattttc gtggtgcttt acaaaacgtc ataggtcgtg gcaaaggtac ccactttgcc
     1141 atctggatga ccggtttttt gtttagtgca tttcatttac agttttatgg ccttttgcct
     1201 cgatggttgc tgggagtgtt ttttggatat atcttggtat ggagtggaaa tatatggctg
     1261 ccagttattg cgcattttac caacaatgca atagcagtga tattttacta cttcagtggg
     1321 caaaacaatg aagtagtaaa cattgatgct gttgggacaa atgatagctg gtggatgggt
     1381 gtaataagtt tgattttaac cattttgctt gttttcagat taaaatcaag aattccaaaa
     1441 aaacagcata tttaacaggc atttcttctt tcgcctacta tcattaaagc ttcctctcgt
     1501 aaaaataaag gtaattctat ctgcctcatt tgtaaactgc gtaaccaacc tgccacatcg
     1561 ctttcttgca aactgtagaa aacttccgaa agctgttcta tttctttatc ggaaaattgt
     1621 gtcggcgatt ttccactcaa agcatcaagt tcttcgtcat cataatattc tatttcattt
     1681 tccatgttta agagattttc tctttcacaa agagcatgtg ctccacagca ttctgaattt
     1741 tctcgaactt gtaccgttgc ttcttctctt ttgccttttc ggctaaaata ggtaactgtc
     1801 attaaaacaa gtccgactga aatcaaaatt attatcaata cccacataat tgtttgtatc
     1861 ctttcttagt tgtttataaa tatacaacaa attttttgac tgtaaagtta gtaataaaac
     1921 tgaaaaaatg tttgttaaat tgtgtatgaa ttttcggtcc cacttatgtt atatttatcg
     1981 tacactaagt tatacaaaaa atattttttt gttttctctt taatattttc ttacctttgg
     2041 ataattttta cagaatgatg tcccactcaa gggtatagat tttgatatct ggtattataa
     2101 attttttttt gtagaatttt gatgcatatt taccattaat ctaaggattt tactcataga
     2161 tgcttaattc ctgaataaat atgattgatc accattcgaa aagagcaaga atatatattt
     2221 ttttaagaaa ttttaaatgg gaaactaatc cgggtattgt catgatggta gttgccatcg
     2281 caacccttgt tattgccaat tctccattga aggaatggta tcagtcattt tggaatatag
     2341 aatttttact tggtacaaat tcattcaacc tttttagtca tcacggtaca ccccttaccc
     2401 tgttgagttt tatcaacgac tgcttaatgg cggttttctt tttttctata ggtttggaaa
     2461 taaaacgaga agttctgatt ggagaacttt catctttcag gcagtcgatt ttacctatga
     2521 ttgcagcttt gggaggaatg attgttcccg ttttgtgctt ttttgttacg ggtcgaatac
     2581 agaatctttc gccagatgaa atgagaggaa tgtttatccc gatgtcaacc gatatagctt
     2641 tttcgttggc agtggtatcg ttgttgggaa aaaaaattcc tgtgagttta aaggtttttt
     2701 tgcttgctct ggcgattatt gatgatattg gtggtattat agtaattgct ttattttata
     2761 gcgatttcaa tgccgaatcg gtaatatatc ttctttatgc tgcatttttt gtgattgtcc
     2821 ttgctcttgg aggcaaattg cgtatcaacg gaaaactatt ttatgctttt tttggtatta
     2881 ttgtatggta tttgttcctt cagtcgggta ttcatccttc cattgccggc gtagtagttg
     2941 cttttactgt tcctgcccat ccgcgtatca acatcagaac ctttatacgt cagctgcgca
     3001 gagatattga tttacttgaa agtgtttcac ctaagcccac gaaggaggaa ataattctca
     3061 gcaacacaca aattaaatat ctttctcata tcgaaaatgc agctgatcag gtaatcagtc
     3121 cgctacaaga ttttgaagac aacctctatg tattagtgaa ttttattatt ttgcctcttt
     3181 ttgcctttgc caatgccggt gtggttatca atctgtccga tttttatttg tcgggtattg
     3241 ctctggccat catcataggt ttggtactgg gtaaaacatt gggaatattt tcctttacat
     3301 ggcttaccat caaatcgggc ttatcgcgta tgcccaatgg catgaactgg cacagtctgt
     3361 ttggtttgtc attagttgga gggataggtt ttacagttgc attattctta actgctctat
     3421 cataccctgt cggaagcgaa atacttaatc aggcgaaatt aggtattatt gcagggtctc
     3481 ttatttcggg ctttttgggt tactattatc tgaagttttc gatcaacagg ggtgaaaaaa
     3541 acaattatcg attgaaaaaa aattcacagg cttcataatc atgtgtttaa acatttgtaa
     3601 gatgcctttt gacaaaaaaa taaaatactc aagggagttt gccaaatatt ttataaagca
     3661 taatttgtaa ctatccgaac tatttttatg gaaaaaatca ttatactcga ttttggttcg
     3721 caaaccactc aattgatagg tagacgttta agggaactta acgaattttg tgaaatactt
     3781 ccctacaaca aatttcctga acaaacaaaa gacataaaag gtataattct ttccggaagt
     3841 cctttttcag tttatgatcc aaaagcacta caaatcgatt tgaataaaat tagagggaaa
     3901 ttcccaattt tgggaatttg ttacggggca caatatattg ttcactcggc cggagggaag
     3961 gttcagcctg gaaatactag agaatatgga cgagcttcgc tcacctatgt ggatgcaaca
     4021 gacccattaa tgtcaaatat tcctgtcggt tctcaaattt ggatgtccca tagcgattct
     4081 ataaccgagg ttccgcataa ctttatacaa attggaagta cgcaggatgt acaaattgca
     4141 gctttccgta tcgagggaga aaaaacctgg ggtgttcaat ttcatcctga agtatttcat
     4201 actacagaag gcctattact cttagaaaat tttttaaata tttgcgattg tcggcgaaac
     4261 tggactcccg cttcatttat agaaactacc attcaacaat taaaagaaca attaaaagac
     4321 gaaaaggtgg tactgggatt gtcgggaggg gtagattctt cggtaacagc cgtgttatta
     4381 aaccgagcca ttggaaaaaa tttgacctgc atttttgtcg atcatggttt gctcagaaaa
     4441 gacgaatttc gtgaagtact cactaattat gaacatttgg gtttaaatgt tattggagtc
     4501 gatgccaaag aaagatttta tgccgtactg aggggaatcg cagaccctga aaaaaaacgt
     4561 aaaattattg gagccgaatt tataaatgtg tttaacgaag aagctaaaaa aattgaaaat
     4621 gtaaaatggc tggcacaagg aaccatttac ccggatgtta tcgaatccta ttccatttcg
     4681 ggtaatacta ttaagtctca ccacaacgtt ggcggattac ctgaaaaaat gcacttaaaa
     4741 ttatgtgaac ctctgcgttt actttttaaa gatgaagttc gcagtgttgg tatcgaactt
     4801 ggaatgacac ctcaacttat caaacgtcac cctttccccg gtcccgggtt ggcagtcaga
     4861 attttgggag aagttacccc tcaaaaagtt gccattcttc aggaagctga ctatattttt
     4921 attcaagaat tgcgaaaatg ggacctctat gataaagttt ggcaagcggg atgtgttttg
     4981 ctgcccattc aagcagttgg cgtaatgggt gatgaaagaa cttatgaaaa cgtggtggcg
     5041 ctccgtgctg ttacttcaac agatgccatg actgccgatt gggcaaaatt gccttatgat
     5101 tttttggcca aagtatcaaa cgaaattata aacaaagtaa aaggtataaa ccgggtagtg
     5161 tacgatatca gttctaagcc tccggcaacc attgaatggg aataatttaa aaggtggaat
     5221 tttcaaccaa atcacagtaa agaattcgct gcacaagtag cagctacaaa ttgtattcac
     5281 gatactgtct aaacattgtt gcactttcaa ggtaaaataa aacttttatg tgataattca
     5341 aacaagcatg atagacaccc atgcccatat atattccgaa gaatttgatg ctgaccgaga
     5401 gaaagttata gagagggctc taaaaagagg cattgaacac ataatcttac caaatataga
     5461 tttgcagagc ttacagccca tgttgcaact ggaagcaagc catcctgata tttgtcatgc
     5521 agctattggc cttcaccctg taagcgtgaa agaaaatttt gcagaaaccc tgaatttttt
     5581 aaaattcaaa ttacaacacc acaactatat tgctatagga gaaataggca tagatctgta
     5641 ctgggacaaa acgtatcaaa atgaacaaat tgaagctttt tgtcaacaaa tagaatgggc
     5701 attggaatgg gaattaccag taattattca tgtaagggat tcgtttaagg aaactatgca
     5761 ggctcttgaa ccctacagaa ataaaaagtt aaggggtgtt ttccatagtt ttacgggaac
     5821 aatagaagaa gcaaaccaga tcataacttt tggaggtttt aaaattggta tcaatggaat
     5881 actgacattt aaaaattctg aactttctaa aactgtcagc caaattgaga cagagtattt
     5941 ggtattggaa accgatgcac cttatttgtc gcccgtccct tttcgtggaa agagaaatga
     6001 gccggcttac ctatcctata tatgtgagca actcgcgctg atacttggga aaacatcgga
     6061 agaagtcgag tttatcacta cccaaaatgc aaagaaattg tttaaattac agccttaacg
     6121 gtaaaaaaaa ataaaaaaaa caagaatctg attataaaat aaaaacaaat atattgtatt
     6181 tcattttttt ttcttaactt gcaccccaaa atggagaggt gctcgagggg ttgaagaggt
     6241 gcgtctggaa agcgtatata cggtgttgaa ccgtatcgag agttcgaatc tcttcctctc
     6301 cgcaacatca aaacaaaaac aaaaaaaaca agcagtcaaa atttaaaaca aaaaattaga
     6361 aatcttaaaa tttaaagaaa aatgaaaaag gtatttgcaa tcttatcgat cgtagcaatt
     6421 ttttctttcg gaagcgctct gcatgcttcg gctcaagata caagcgctcc tcaaaccgag
     6481 cagacagccg gcaatgatac ggctgtagca gctgcacaag caatggaaca gatagaagcc
     6541 cctgccccag cacaaggtgg tggcatgcat aaggctttaa aacaaaaatt tatcgaagga
     6601 agcccggcat ggatggcacc tattgctctc tgcttaatta ttggattggc tttgtgtatt
     6661 gaacgtataa tttatttgaa tcttgcttct accaacacca agaaactctt ggatgatatt
     6721 gaccaagctt gggctaaagg aggtgttcaa gctgctatgg aagtttgtag aaataccaga
     6781 ggtccggtag cttcaatttt ttatcaggga ctttcacggt acgatgaagg aattgatgta
     6841 gttgaaaaag ctgttgcttc ttatggcggc gtacaattag gattgctcga aacaaactta
     6901 acttggatta gcttgtttat tgctatttca ccaagtttgg gattcttagg tactgttatc
     6961 ggaatgattg aagcatttga tagaattcag caagtaggcg atatttccgc aacagtggta
     7021 gccggaggta ttaaagttgc tcttttgaca acggtatttg gtcttatcgt tgctatggtg
     7081 ttgcaggtat tctacaatta catccttacc attatcgaaa acatgaccaa tgatatggaa
     7141 gactcttcaa tttcattgct cgatatcatt gtaaaacatt cgaaaaaata aattcgtaaa
     7201 aacttaataa ggttatgtcg aaattcatgc aaaaaatacc tataatttca gcaattttcc
     7261 tggtatcggt atcggtaatc ttggtattgc tgatttatgt agggggaacc tcaggttcga
     7321 taaacaatag cgccggtgag gcaatgtcag ttccaagatt tactgatgca ctaatttact
     7381 ggtcatattt tcttgtggga ttagctgtag ttattacatt gggctttgta gttaaaaatt
     7441 ttttccaatc ttcaaaacaa agttcatcat ctatttttaa gtctcttctg cctatcatag
     7501 gatttattct tttgttcgtt gtttcatggc tacttggcag tgatcaggaa ttgaaaatta
     7561 tcggatatga aggagcagag aacgttggat tctggtcaag atttaccgac atgatgattt
     7621 ttccgattta tgtaatggca ataggaatta tattaacaat cataggtact tctatttatt
     7681 ccagtctaag aaaagattaa aactgaattt tgagttaata aaagtttgac tcttactaat
     7741 aatatattaa aatgtcgaag aaaaaaagaa aagtaggtca aataaatgcc agttcaatgg
     7801 ctgacatttc atttttactt ttaatctttt tccttgttac tacatccatg aatgttggaa
     7861 cgggtatcgc aagaagattg ccgcctccgc tgcctcccga tcaaaaacca ccagacatgg
     7921 acataaaaaa acggaatatt ttcattgtcc gtatcaacag ccaaaaccaa attatggcac
     7981 aaggagaaat attagatata aaacagttaa ggtataaaac aaaggaattt ataaaaaatg
     8041 agaataatga ccctaatttg ccggaactga ttactgaaaa tattccctta ttgggagaag
     8101 ttacttatac taaaaatcat gtgatttcac ttcagaatga tgtggggacg caataccaag
     8161 cttacataga tgtgcaaaac gaattgattg cagcatataa tgaactgcgc gatgagtttg
     8221 ccatgcaacg ttttgggaaa aaatacaacg agttaagcca agagttgcaa gaagcagttc
     8281 aaaaaatcta tccacaaaaa atttcggaag ctgaacccaa aaattatgga ggagtaaaaa
     8341 gtggcaagaa ttagaaaaag cgaaaaacga gaaatgccgg cattgagtac ttcttcttta
     8401 ccggatatca tcttcatgct gttgtttttc tttatggctg ttacaaccat gaaagaagta
     8461 acctataagg tcaggattga tcctcctaaa gctacggaaa ttcagaaatt ggaaaacaag
     8521 tcgctcattc gatatatcta tgtcggtaag cctttggagc agtatcaaag aatttatggt
     8581 agcgaaaccc gaattcagtt gagcgatatg tttgcagata tttctgaaat agaagagtat
     8641 atcattaacg aacgtagtgc aatgaaggaa gaagatcaga atttaatgag tgtatgcatt
     8701 agagctgaca aagatactca tatgggtatt atcactgata taaagcaagc tttgcgaaga
     8761 gcctacgcac ttaaaattat ctatgctgcc aatcagcgta cacagtatta atcttcttag
     8821 tttttagtgt tatccaaata ggctgtctca taagtgcgac agcctatttt ttttgtgctt
     8881 tgcaggatat agaaaaattt attttacaaa taacaccaat tggtaaaatt caaccgcatt
     8941 tttacctaaa tcaaaaacat tattttgaac gttttaaatt gagatgagaa tcaagatgag
     9001 aattcgtgaa agtatcattc ctttttcaat cacatttttt aaaatatact caataaacgg
     9061 ccggttgcag ggtcaataat tactattttg tcattacctt tgatcatgga agacgatagc
     9121 ggaattacaa tgccaaactg tgggaacaaa aattgttgag aaaaaaatgt gcttttccca
     9181 tcggaaacag taaccaatgt agcagaagga ataacaaagt ttatgtattc tttgggtgct
     9241 ttttgtttgg ggttgtccaa tatggttgga gatttagtag gaataacact aatatagtac
     9301 ggaacgccac tcaaatcatc agctggcaca agccctttta aaaccgaaaa acgaaaaagg
     9361 acttcgttgt tcaaagcttg ttgaggtcgt aaatataagg tatgtttttg aatttctttc
     9421 gttgtactac caataaaaag ttctgttaac tctttttctt tgttatccaa ttcatttaaa
     9481 atggcactca atgatgttcc atcagaaggg aaatgatcaa cgtctccggt aagtaagttt
     9541 aatctgcttt cccgaattct atatatctgt ttggcagctc cttctgcaag ttttgctact
     9601 gacccaacca acatatattc ttcacccagc ggcaatataa cgttattgct aagattttct
     9661 tttgtttctg taggtgcaag cactgaaagt tccggcaaag aggtgggagg aggaacattt
     9721 attccacata ataagccgtt ttgatcaacc gagatggaca caaagggcga atttttatcc
     9781 actctaacgg cataggtttt cgatggatcg ggaacggcat ttgaagttac ctgaatatct
     9841 tttaaagaat aaactgtttt ttcttcggta attatatctg aagtagccaa atatctttca
     9901 gaataacgat aaaaaacacc gggttttcgg gtaatttttt ccacgtcaac tttaatacat
     9961 aattctgtca tgggtaaaga gtaaataagc actgactcgt ttttattcaa tgtgagggaa
    10021 gattgacata acacgctcga aataacagaa gccataagga atgaaacaat tatttttttc
    10081 ataacactga atttggttga ttgatgttgt taataaagca ggaaatatat aaaatattca
    10141 gaaagatttt tcgacttttt gaaaaaattg accttaaagg atgtttcttt atgtcatgct
    10201 ataaaatcaa ttttattgaa attcattgat ttctgagcaa atatagaaaa acaggtgcaa
    10261 attacaaaaa agaaaatcta aaaaatattt cagttggtaa gaagaaagta taaatacttt
    10321 agaaacatgt tgttgttacc gatttataaa aaatcttatt tctgttctac aacgcagaac
    10381 gtttgtaaaa gtataaattt atttttatta tctttgtcca gcttaaaaaa aatcatttct
    10441 tggctgcaaa aaaggcaata tgccttactt atatggaaga attttaatgt cgatttcata
    10501 aaaaagaata aattgcctta ttaattttat gggtaaaatt atatcgttgg ccaatcagaa
    10561 gggcggagtt ggtaaaacta ctaccgccat taatttagca gcttctttgg catcgttggg
    10621 taaaaaagtt ttgctggttg atgccgatcc tcaggcaaat gcctcttcgg gattgggaat
    10681 agaaattcaa tcgctgaaat ataccattta cgaatgtttg attgatggtg tgtcgccaca
    10741 gcaagctatt catggaacgg aaatagaaaa tttgtcggtg ctcccttcac atatcgattt
    10801 ggttggagca gagattgaaa tgttgaacat ggaggagcgg gaaaaagtga tgcagcattt
    10861 gctacgcccg ttaaaacccg atttcgattt tattttgatt gattgctccc cttcattggg
    10921 tttgataacg gtaaatgcac ttacggcttc cgactcggtg attattcccg tgcaatgtga
    10981 atattttgca ttggaaggta tcagcaaact tttaaatacc atcaaaataa ttaaaagcaa
    11041 gttgaatcct tcgcttgata ttgaaggctt tttgcttacc atgtacgata atcgtcttcg
    11101 gttggcaaac caggtttatg ccgaagtcaa aaaacatttt cagaatatgg tttttgaaaa
    11161 tgtcatatac agaaatgtac ggctcagcga agcgccaagt tacgggaaac ccgttatgct
    11221 gtacgatccc gaatcgaaag gttcttccaa ttacctcgat ttggcaaagg aattgattga
    11281 ccgacaaaaa agttaatata gcttgcaata aaaaataaaa agaataaaat ctcgtttgca
    11341 gttatggtaa aaaaatcagc attgggaaga ggacttggcg cgttgatcga caccgacgac
    11401 atttatacca gtggttcgtc ttccattagc gaagtggatg tggatttgat ttttccaaat
    11461 cccaatcagc cacgttctca ctttgatgag gaatctttgg ccgaactggc ttcttccata
    11521 cgtgaactgg gggttatttc acccattacg cttcggaaaa atgatgatgg aacgtatctg
    11581 atcattgcgg gagaacggcg ttatcgtgct tctaaaatgt tggggctcaa atccataccg
    11641 gcctatgtta aaacggcagc cgacgagcaa gtggtggaaa tggcactgat tgaaaacatt
    11701 cagcgcgaag atttgaatgc cattgaagtg gctttggctt tttaccgact gttggaagaa
    11761 tatcatctta cgcaggagca gcttagcgaa cgagttggca agaaaagggc taccattgcc
    11821 aattacttgc gtttacttcg tttgccggcc gaaatccagg tgggaattaa ggataaaaaa
    11881 atcgacatgg gacacgcccg tgctatcttg ggattggaag atcctgctgt acaacttcaa
    11941 ctctacgaag ctattctgga aaatttttat tctgtcagaa aagtggaaga aatggtcaga
    12001 ctctacgccg aaaccggaag ttttgaggaa aaacatgttc gcgtttccaa acccaatccg
    12061 ataagaaaat taaaagaata cgaagaatta aagaatcaac tgagtaaaac tttcggcact
    12121 aaagtacaat ttagttgtaa tgagcaagga aagggaaaaa tttccatccc attttcaaac
    12181 gatgaagaat tatcccgtat catgggattg ctggataaaa tttaaaccaa atttcaagtg
    12241 aaaagatttt ttctgataac ggcagaaata tggtttatct cccttttcgg aattgtactc
    12301 aatgctcaga atgtcaacga aaaaagagag attgttcagg atagtataaa tcggctgcct
    12361 gaaaaatctt tacaaaaaga aagccaagta tcaattgata ctgtacaaaa gccggtttac
    12421 atgcccaatc cgtcgaaaat tgtttggatg ggtgcagtag taccaggttg gggacaaata
    12481 ttgaacagga aatactggaa attgccaatt gtgtatggcg gattgttggg atgttattat
    12541 gcctattcat ggaatgcaag ccggtataat tcttatcata ccgcttattt ggatattatt
    12601 gattcagacc cgacgacaaa cagttttctg gatattttgc cggcaggata tactcttgaa
    12661 tcaattggcg ggaccggcag atatacaacc attttaaaaa gcggacagga tcaatttcgt
    12721 cggcagagag atttgagtgt aattgtttcg gtggcttttt atgctcttac gttggttgat
    12781 gcttttgtgg atgcacaatt gtacggcttc aacattagtc ccgatttgtc gcttgaaatc
    12841 aaaccaattt atctaaaaga taatataact acatcagggg ggcttggcat gcaatgccgt
    12901 tttaagttgc aataattacc ccgcattatt aggataaaaa atgaatttaa aattttccat
    12961 aattgttgtt tctttcattt gcttttcgat aaattcagtt atttcacaag ctgccaaaga
    13021 tttcccggaa gatactttat cttccaaaat cgacagcatc attggaatac ctgacgcata
    13081 cggatatgca ttagatcatt tgcttcatct atgggcattg cagcgaatgg atactacaga
    13141 atgcaattcg ggtgagccac ccgcaccggt ttccgattct ctatataaat atcggctctc
    13201 acaaatacct tgtttgatag agcttcctta taatagtatt gttcgttcct ttatagaact
    13261 ttatactgtc cgaaagcgaa ctcaaacaga atacatgctg ggcatcagca attattattt
    13321 ccctgttttc gaacaagcat tagatgccaa taaattgcct ttagaattaa aatttctccc
    13381 tatcattgaa tctgctttgc accctactgc tatttcacga gctggagctg ccggtttgtg
    13441 gcaatttatg attccaacag ggcgaatgta tggtttggaa atcaacacat tggttgacga
    13501 acggcttgat cctcgaaaag cgtcctatgc tgcagcgcgg tttcttaaag atttatatgc
    13561 catttacgga gactggaatt tggtgattgc tgcttacaat tgcggtcccg gtaatgtgaa
    13621 caaagccatc agaagagccg gcggaaaaac cgattattgg gcaatttacc cttatttgcc
    13681 attggaaaca cgaggctatg ttcccattta tattgctgcc acttatactt ttcattatgc
    13741 cgaagaacat aatttatgtc cagcagtagt aaatattcca gcactaatcg acactttcat
    13801 gatcaatcaa agaatccatt tggaacaaat atcgtcagtg cttaatattc cgattgaaga
    13861 gctccggtgg cttaatccac aatataaaaa ggacataata cccggtgatt taaaacccta
    13921 tgtcttaact ttgcctgtaa attattccag catttttatg gagagattgc atgaaatagc
    13981 ttcttataaa gctgatgaat taatcaataa ccgtagaaac gaaattgaag ctttcaggtc
    14041 aacttcttca tctaatttag ggacttccaa aaatgttact tatcacaaag taaaaaaagg
    14101 tgagaccttg agcggcattg ctgcaaaata tggtgtttca gtctccaacc ttaaaaaatg
    14161 gaataacatc aagggtagta aaataacaat tggccaacgt ctgaaaatta tcaaataaaa
    14221 ttcaacatgg aaaaagtata ttattccatt tctgaagtag cagaaatgct gaaaatcagt
    14281 ccttccaacc tgcggttttg ggaaaaggaa ttttcgcagc tgaagccaaa acgaagcaac
    14341 aagggaacac gtacttatac caaagaagat attgaaatta ttaaaatgat tcacttactg
    14401 gtcaatgagc agaaactcac attgcaaggc gcaaaacgta aactttcgct gaaaaaggag
    14461 accgtttcca aacagcagga gttggtggaa cgtttacaaa atgtgagaaa ggaattattg
    14521 atgatcagta aggctttgga tgaataggca atttacaaat aaaatataaa atgaaaaaaa
    14581 cgaatttttg tttaggcttg atagctgcat taattttgat gtcagcttcg gtttctgctg
    14641 agaaaaagcc aaaaaacgta attctgatga ttggtgatgg tatgggttta gcccaaatgt
    14701 attcggcaat gactgccaac aacaatcaac ttcagctcga acggtgcact tacactggtt
    14761 tttcaaaaac ttattcttcc gatcattata ctaccgattc aggtgccgga ggaacagcca
    14821 tagcttgtgg cgtaaaaaca aaaaacggca tgattggcat gacgcccgat tccgtaaaaa
    14881 tagaatctat tctcgaactc tccgaacgca atcagttgtc caccggaata gtagtaactt
    14941 gtgcagtaac ccatgcaaca cccgccagct ttatcgcaca tcagattagc cgcaatatgt
    15001 atgaagaaat agctggagat tttcttcaaa cagatgttga tgtgttcatt ggtggcggac
    15061 tgaagtattt taataacaga aaagaccaga taaacgtagt agaaaaactt aaaaaaaata
    15121 attatcaagt agcttattca ctcgacgaag ctgtaaaagt aaaatcggga aaattggccg
    15181 gattgtttta tgagaatcat tgcccgccga tgcctgagcg tggcgatatg ctcccaaaat
    15241 cggtaatggc agccatagat attttggaca acaacaagaa aggttttttc ctgatggtgg
    15301 aaggttctca aatcgactgg gctgctcacg acaacgacaa gaaacagatc gtaaaagaga
    15361 tgttggattt tgatgaaacc attggaaagg ttttggatta tgcccagaaa gatggcaata
    15421 cattggtaat tattacagcc gatcatgaaa ccggcggaat gactatttta aatggaaaat
    15481 tcggatctga cgaaatggat gcccattttt ctaccaaaaa ccactcgggt gttcctgtgc
    15541 cgatctatgc ttttggaccc ggagccgaac agtttaccgg tattatggaa aatacagcgt
    15601 ttaaagggaa aatagaaaag ttactcgatt taaaaaaata atttttcatt attacttgtt
    15661 gaacagttaa atttttaaca gttcggtcgt aaatttttct gatatattca ttttcaattt
    15721 aataaaaaga caatataatg ataacaatcg aacaacttaa agacgttaca gaacgcaccg
    15781 aagcgttgag gaggtatctt tgacgtcgac aacaggcaaa ttcaaataga agaagaagaa
    15841 cttcgtacgc aggaacccaa cttttgggac gacccgaaaa aggcagaagc tcaaatgcga
    15901 aaattaaaag agcttaaaaa ttgggtaacc ggttaccagg aagtgaaaac cgcagcagat
    15961 gagctggagc ttgcttatga tttttttcgt gaaggcgtga ttgaagaaca ggaattggac
    16021 gaaacctatc ggaaggcttt acaattgatt gaacagttgg aaatgaacaa catgctttct
    16081 catgaggaag atcagttggg ggctgttctt aaaattgttg ccggagccgg cggaaccgaa
    16141 gctcaggatt gggcggaaat gctgatgaga atgtatcagc gatgggcaga aagaaaaggt
    16201 tataaatggg agattaccaa cttccagccc ggcgatgagg caggaattaa atccgtaact
    16261 attcagatag aaggagaaag agcttacggc tatctgaaaa gtgaaaatgg cgttcaccgt
    16321 ttagtcagag tctctccttt caatgctcag ggaaaaagaa tgacttcttt tacctcggtt
    16381 tttgttgttc cgctgattga tgattccatc gaaatagaga ttaatccggc caatctttcg
    16441 tgggatactt ttcgctcgtc gggagctggc ggacaaaatg tgaataaggt tgaaaccggt
    16501 gtaagattgc attacacctt taaacatccc gtaacagggg aacagagcga aatcgttatt
    16561 gaaaacaccg aaagtcgttc acaattcgat aacaaggaca atgctctgcg tttactcaaa
    16621 tcgcaacttt acgagcttga acttcagaaa cgaagggctg aacaggagaa agttgaatcg
    16681 accaaaaaga aaattgaatg gggttcgcag attcgcagtt atgtttttga cgaccgtagg
    16741 gtgaaagatc atcgaaccaa ttatcaaacc tccaatgtgc aggcagtgat ggatggtgat
    16801 attgatgaat ttatcaaagc ttatttgatg gaatttcccg actaagagaa aaacgttaat
    16861 tgctttttcc caaattccat attttgatcg aaaatgtttg tcttcaaaaa aatggagtag
    16921 gggatatctc ttttttctat ttaaacccag attactcatt aaccaattca aagggaaagt
    16981 caaagtcaga agagttgttc tatagtcagg aaaactaagc tcgccttttt tatttattta
    17041 aatggatttg ataactggtt tgctgttgat tttttcatga tatgcattcg actgcagaag
    17101 ttctatatct ttagataaag ttattttata caaaattaaa accctttttc tattgaggat
    17161 tctgagttaa aataagacat cggacaaaaa aattgcaact ttcaatattc aatttttttt
    17221 gcgaaatttg caaacttaaa gaatttaagt aaaaacgcca aagtgaaaga aactaaatac
    17281 atttttgtta caggaggtgt agtttcctct ctgggaaaag gaattattgc agcttcgttg
    17341 gggaaactcc tgcagtcgag aggttttaag gtgaccaacc aaaaacttga tccttacata
    17401 aatattgatc cgggtacatt gaatccttat gaacacgggg aatgttatgt gaccgttgat
    17461 ggacatgaag ccgaccttga tctggggcat tatgagagat ttttgaacat tgagacccat
    17521 cgtgcaaata atattaccac cgggcgaatt tatcaaaatg ttattgaaaa agaacgtcgt
    17581 ggagattatt tgggaaaaac cgtacagata attcctcaca ttacggatga aataaagcga
    17641 aatattaaat tattgggctc aaaaggcgat tacgattttg taatcacaga aattggcggt
    17701 actgttggcg atattgaatc attgccctat attgaaagtg tccgccagtt gcgatgggaa
    17761 ctcgggaaaa attgtttgat tgttcatttg acttatgtac cctatatttc agctgctaag
    17821 gaactaaaaa caaaacctac tcaacattca gtcaaagctt tacaggaaca aggtgtacaa
    17881 cccgatatcc tggtattgcg taccgagcac aatatttctt ccgatttgcg taagaaagtg
    17941 gctttgtttt gtaatgtcga acccaattcg gttatgcagt ctgtagatgt ttctaccata
    18001 tatcgtgtcc cattaatgat gtacgatgaa aaacttgatg aagtagtact tagaaaaatg
    18061 gggtttgact tgttgaaaat acctcaggcc aatctttcca aatggataca gtttgtggaa
    18121 aaattggaaa atgcaaaaga agaagtacac attgcattgg tgggcaaata tgttcagctt
    18181 cccgatgctt ataaatctat tatcgaatct ctacttcagg catgtgctta taacgacaga
    18241 aaattaaagc tcgatttaat attgtcggat aaacttaccg aagaaaatat agaaaaaaaa
    18301 cttgagggag tcgatggtgt tattatcgct cccggttttg gtcaaagagg aattgaggga
    18361 aaattagtca ctatcaagta tacacgtgaa aaaaacatac caactttcgg catttgtatg
    18421 gggatgcagt gtatggtgat agaatttgca agaaatgtgc tgggttatgc agatgccaac
    18481 agcaccgaaa ttgacccgac taccactcat aatgtagtgg acattatgga agaacagaaa
    18541 aatattttga atttgggtgg cagcatgcga ttgggagcat ttccctgcaa attgaaaaag
    18601 gggacaaaag tttttaagat ttacggaaaa gaaaatatca gcgaacgaca tcgacaccgg
    18661 tttgaattta acaactatta taaagaagag tttgataaag ccggcatgat ttgcagtgga
    18721 ataaatgctt cttcaaattt ggtagaaatt gttgaagttc cttctcatag atggtttatc
    18781 ggcgttcagt ttcatcccga atacagcagt acagttatta atccccatcc tctttttatc
    18841 agttttataa aagctgccat cgaatataga aacgaatcga aggaaaaaac tcaaaactca
    18901 aaatttaaaa cagctattaa ataattaatg attgaaaatt taattctcat ttctcacttg
    18961 ttgaatttca aaaatatatc atggataaaa atacaattac aggttttcta ttaataacgg
    19021 ttattattgt tgtctttgcc ttattgaatc gccccaatca ggaaatgatc gaaaaacaaa
    19081 gacgctacaa cgattcgata gcacaaattg aaaaagccaa agctgaatcg gaaactgcta
    19141 ttcaagaagt caattcttta ctttccgatt ctattaaaac cacactggat tcttcggctg
    19201 ttatcagaga tgcttatggc gatttcagtg ttgccgcttt agggaaagaa gaatttataa
    19261 cattggaaaa cagcgtttta aaacttacct ttacgaacaa gggggctcgt atttatgcag
    19321 ccgaactaaa agattatcgt cgacatgatt cccttcctct gattcttttt caggggagtg
    19381 aggccaatat gggatttact ctgataacca acaataacag aattctgaac acagccaact
    19441 tattttttga agttttactt cccatagaaa aagatacatt gggaaatcaa atacttgttt
    19501 tcaggctaaa aactaccggc gaaggataca tggatgtggt ctacactctt cctcccgaca
    19561 attatatgtt tagtttcagc attaaagcca acaaaatgag caatgttatg ccggcgggaa
    19621 ctaatttttt ggaaatgcag tggactaccc gattgcgtca gcaggaaaaa ggtcggcaat
    19681 ttgagagtcg ttactcaaat attcagtata aatatctttc ggatgatgta gaaaaattaa
    19741 gtgaaagtaa aaatgacagc aagcaaatta ccaacaaact gaaatggatt gctttcaagg
    19801 accagttttt cagtaatgtg cttatagccg aagatgcatt tacttcgtct tttctggaaa
    19861 gcaaggttga acctgaggat tctccctatc tgaagtcgta cagggccgat atgaccattc
    19921 catttgaccc taccggaaaa acttcgacca actttagaat gtatttgggt cctaataaat
    19981 tcaatatcct tgcatcttat gacaaaggag tagccaaaga agaaaagctt caacttcata
    20041 aactgatacc acttggatgg ggaatttttg gatggattaa ccgttacatg attattccca
    20101 tgtttaattt cttcagtaaa tacatcggga attatggcat catcatctta ttgatgacaa
    20161 ttatcattaa aatcataatt tttcccatga cgtataaatc atatacatcc agcgccaaaa
    20221 tgcgagtcct taaaccagaa attgatgaaa tttacaacaa aatacctcct gaaaaagcta
    20281 tggaaaggca aaaagcagtc atggatcttt attccaaagt tggtgtaagt cccatgagtg
    20341 gatgtcttcc ggctttgttg caaataccta tcctttttgc catgtttagc ttcttccctg
    20401 cttcgatcga actccgtcag gaaagttttc tttgggcaaa agatctttca acttacgatt
    20461 ctataattag ctggaatgct tatatcccaa ttatttcgaa gtattttggc aatcatttaa
    20521 gccttttctg ccttttgatg accatttcaa gcattatttc caccaagttg acaatgacaa
    20581 ctactccaac taccaaccaa ccgggcggaa atttgatgaa gtggatgatg tacttgatgc
    20641 ctgtgatgtt tatgttcatt tttaacaatt actcggcagc attaagttac tattacttgc
    20701 tttctactct gattaccatt attcaaactt ttatcattcg ctcaatggtt gacgaaaaga
    20761 aacttttagc ccaactacat gctaaacgca atcagaagca gcctgtgaag aaaaaaagca
    20821 gttggattga acgaatggaa aaaatgcaac gcgaacaaca aaaagcattg aaaaacaaaa
    20881 gataagagtt ttagaaaata atatgtttaa gcgtaaaatt gcataaaaaa tttttgtggt
    20941 tttacgcttt cttttaaaat tcaatcatga caaaaaaaat caagtttttt attctgtttt
    21001 ttctgattat atttcttttc tcgtgtcaaa atcataaccg ttttcgcatt aacacaaaaa
    21061 agaatgaagt tgaagttcgg atacggcgtt ttgataagga tttgatcaac gttgacacta
    21121 caaatctcaa agcagaaatt aggaaaattt acaaagatta tcccgttttt ttcccggtat
    21181 ttattaacga aatacttaac gttgcacctt ccgacaccga ttcagttgct aaattaatag
    21241 gagattttct ttgtgatgcc gcttttcaaa aagtcaacaa agaagttctg aaaacttttg
    21301 ctgatgtgca tgatatagag aaacaacttt cgatagcttt tacttatatg catcattatt
    21361 ttcccgagat caggctaccc gaaatttatt ttttcgtatc cggcttcaat cgttctatca
    21421 ttatttaccc ttctttaatt gctgtagggg ccgatttata cttaggagcc gattaccctc
    21481 tttataagtc gtttacttat gaatacctga ctttcaatat gcgacctgaa agtatagccc
    21541 ctgatgttgt ttctacgctt ctttttagtt atttcccaat ggaatcggaa aataatcgct
    21601 tgttggacaa tatgctgtat aatgggaaaa ttttattttt gttgtcggtc tttatgcctg
    21661 atgtagaacc ttcagtattg atggggtata ctcgcgaaca atggcaatgg tgtaaacgat
    21721 ttgaaaagga aatatgggca aaaattattg aagacaaagc attgttttct tccgatatga
    21781 ggcttataaa ttcctatttg gatgaagctc cttttactgc tcctgtatca caagattctc
    21841 caggtagagt gggcacatgg ataggttggc aaattgtgaa tagttatatg aaaaaaaaca
    21901 ataacgtcag tctacgtcag ttagctaatg aaaataatta tcagctactg ctcgagaaat
    21961 cggactaccg tccataaaaa aagcggttcc gtcatgcttt aatgacagaa ccgcaaacaa
    22021 aataaattaa atcgaaaatt taattatgca ggtatgattg cttctgatgg acaaacatca
    22081 gcgcatacac cgcaatctgt acacacatcc ggatcaataa catagatatc gccttctgag
    22141 attgcttcta ccggacattc agttatgcac gatccacatg caatgcaatc ttctgtaatt
    22201 acgtaagcca tttttatata gatttagttt aatgctgcaa agttactttt ttttctcaaa
    22261 ccaaaaaaat ttcacaacac ttattcactt ttgaaatttc taaagtcgta atttttttat
    22321 caggtgagag acagattatt ttgttatgct cacaaacaaa tttttccttt catcagaata
    22381 taatttattt atttagagtc tgatggaacc cacatgtctg aattattata ttcattcgtg
    22441 tttgtgtatg caatacatgt gggtttcatg ttgaatttta aaatctcatt aagcctttaa
    22501 ttctgagatg ggatatttta tcttacattt ctaaagtaaa ttgttgaaac atatcttaaa
    22561 cattggtatt acataccaaa gtcaattatc attgtttgga atttatcaca tccagcgctt
    22621 tcaatacagt tttgtcgctt cgataaagta atggataaaa tccttcatcg cccagaatat
    22681 ttcgcgaaat atacccttta atttgtgtca aaagcaaatt ttttgagatg tttatttctt
    22741 ttttattggc ttgaattcct ttttcggaaa caaattttac aaattgatcc aataaggatt
    22801 gactatttaa gtaagtgtcc atttcgtgcc aattctttat ttttttcagt ttctctctgt
    22861 ttttgtcaga atattgaaag gcaaactgat aaacatatcc gtaattgacc actttattaa
    22921 aatatggtgt aaattctgat gtatccaaag gaacatatac atcgggcata atgccgcctc
    22981 cgccatatac aattcttcct ccaaccgttt tgtatcgcaa agaatccggg tggaaaatac
    23041 tgtcgttaat ttcatgcaac agacggttga taaattcttt ttcgtattct tccgaattac
    23101 caagtttata aggcttttga atacttcgac cggaaggggt ataatagcgt gccaccgtta
    23161 ggcgaacagc cgaaccatca gacaaattaa actgctgttg caccaatccc ttaccaaaag
    23221 tacgtctgcc tactaacgtt ccgcgatcgt tgtcttgaat ggcccctgca aaaatttcac
    23281 ttgccgatgc agaaaattca tcaacgagta ctaccagcgg ctcccttgta cagctaccct
    23341 ttccatctga tttggcttca aagcgcttat aggctttccc ttgtgcataa acgataagtc
    23401 ggttagccgg cagaaattcg ttaatcatat taatggactg atccataaat ccgccactat
    23461 tgcctcgcaa gtcaattatg aatttatcag caccctcgtt ttttaatttg gctatggcat
    23521 tcagaaattc tccataagtt gtttcggcaa atttgctgac tttaataaaa cccacacttg
    23581 gctgaacaat ataagcaata tctacggaat ttacaggaat ttttcctctg gttatttcat
    23641 aatacaacac ttcctttgtt tggtttcgtt taatgcccaa cttaactttc gtaccttccg
    23701 gtccgcgaag ttttttcatt actttttcat tgttaatact ttttcccaca aaggtggtgt
    23761 catttacttc tacaatgcga tctccgggca ttaagccagc tgtttccgaa ggacctccgc
    23821 tgatcacgga tataatcatg actgtatcgt tctgaatgtt gaattgtacg ccaataccac
    23881 taaaactacc ttccaattcg ctatttgcca aagaaatatc ttctgccggt atgtataccg
    23941 aatggggatc aagttttgcg attatatctt gcatgacgtt ttcagtcatt tcatctaagt
    24001 tgagtgtatc gacataatta gtgttaataa tctgaagcaa ttcatctact ttggaagcat
    24061 taccaaaacc gaaaaaaatg tttggtgaag acgagaaaaa agccctctga gccagtaagt
    24121 tacccagaat aagtccaaaa attacggcta agaatacgat gaggggtgca agataatttt
    24181 ttttgttgcg atccataaat aaaaattatt ttttatgttt tccaaaatat ttatttaaat
    24241 gacattgaaa tgatggtaaa tttcatctct tatttatgaa ggtttttgtt ctcgccggta
    24301 attttcattt ttatcaaacg aactaattct gcttcaaaaa atgattatct cataaataag
    24361 tggataaaat ggtggaaaaa ttaccgaaaa aatttactta aaagatgaat acaaaattag
    24421 agaattaact ttgtttttct gtaaacattt tactaaaaat aacgattttg accgactttt
    24481 gttctttgaa ttgtttttct ttgattttat acaatggttt cctatatccg ttggtctact
    24541 ttctctgtaa aaaaaattat tggcaaaaac attttcatta ttttaaataa aaacacctta
    24601 tatttccacg ttaattatta aatttgcgaa cttaattata aaaccagaaa aaatgaaagt
    24661 acctgaaaat ttgaaataca ccaccgaaca cgaatggatc agggtgcaag gtaatactgc
    24721 atgggtggga attaccgatt ttgctcagca tgaattgggc gaaatagtat ttgttgaggt
    24781 agaaaccttg ggagaaaaca ttcatgccgg cgaagttttt ggaacagtag aagctgtcaa
    24841 aacagtttcg gaactttttt tgccaataag tggtgaaatt cttgaattta ataaagaact
    24901 cgaaaacaag ccggaactgc tcaacgatga tccgtatggc gaaggctgga ttgttaaaat
    24961 aagggtcacc gatccttctg aactggaaaa attactttcg gcagaacaat atgccaagtt
    25021 gattgcttga acaagaagtt ttataaatca attttttaaa aatgaaagca agagcaagta
    25081 ttatcatggg tagcacctcc gatctgaaaa taatggaaaa ggctgccaag cttttggatg
    25141 aatttgaaat tccttttgaa atacatgcgt tatcggctca tcgtactcca aaggaggtag
    25201 aagattttgc tcttcatgct gaaaaaaggg gtattgaaat aattattgcc ggggcaggta
    25261 tggcagcaca tttaccgggt gttatagctt cgatgactac tttgcctgtg attggtgttc
    25321 caattgaagc ttctttaaac ggtatggatg ccttgttttc catcgttcaa atgcctccgg
    25381 gtatacctgt tgctactgta gggatcaatg ctgcacaaaa tgctgcaatt ttagctgttc
    25441 aaatattggc tttaaaagac gaaaaaatca attccaaatt aaagctgtac aaagaaaata
    25501 tgaaacaaaa aattgttgaa gcaaacaatc aactcaaaga aataaaatat acttataaaa
    25561 caaattaatt agctccgtaa actatcactg agaaagctga aattatcatt aaagttttta
    25621 ttcctttttg tttcattttc tgcgaagtat tatcactatt tcacgaagtg tgatgtaaat
    25681 taaaaagttg tataaaaaat taaaatctta ttctttcgac aaggataaga aaattgatta
    25741 agaataattt tccacttttg aataggtttt gttcattttt tagcatctac cctgagggta
    25801 aagaaaacca ttttataacc ccgtcgcccg aagaaaacga cattttattt ttggtttttt
    25861 cccctatttc ccctttttca agataaccaa cactgtatct ttttgtttcc agctttgttg
    25921 taatattgcc caaatttcgt acctttgttc tatggctaaa tgccttcatc atggcaattt
    25981 tttaagagga agagaccaaa gtaatctatt tgttcgataa ccaatgagaa aaattcttct
    26041 atcaacttta acttgaaaat aattctcttt tgcttttatt tcccacgaaa aaattgcatg
    26101 aattacttga atttacgttt gttttataaa gagtgttttt taaaatatga ctacctatcc
    26161 ttctcagtct gtttttagtt tataccgtcg catggtaact tctgaaaaga acatgttttc
    26221 ttttcaggaa aggaaggcca tcaaacaaat tctgaaggaa tactatctac tccatccgca
    26281 gcaattgaca ttaccgggcc attctatcga agacaaattg tatattattc agattctgct
    26341 caatgaaata ggattgggaa aagcttctgt tctgagtgtg ctttttatgg aaccgatcaa
    26401 gaacaattat ttgactgttt cagaaattga acaaaaattc aaaactccgc ttgactccat
    26461 aataggcgga atgcagcgtg tagaagagct ttacgaacgc agtttttcgc tggagacaga
    26521 gaatttcaga aaacttttat tgacttttgc tcaagatgtt cgtgtcattt taatgatgat
    26581 agccgagcga ctttatttta tgcgaatact ggataaattt cctgcagata tgcagcaaag
    26641 catggctcgc gaatgctcgt ttctttatgc tccgctggct catcgtatgg gactttattc
    26701 cattaaaagc gaaatggaag atttgtcgct gaaatataca aatcgggaaa tttatcgtga
    26761 aattgcccaa aaattgaatg aaaccaaacg ttctcgggac aagtacattg aagaatttat
    26821 tacccctttg aaggaaaaac ttggcaagga aattaaatat cccttcgaaa tcaaaggtcg
    26881 gacgaaatca atttattcca tttattccaa aatgctgaaa cagaatacgc cttttgaaaa
    26941 catctacgat ctttttgcaa ttcggattat tctggatgta ccgttggaaa aagaaaaaat
    27001 gatttgctgg caggtttatt ccattgtaac cgacatgtat cagcccaatc cgaaacgttt
    27061 gcgcgactgg ctttccatac cgaaatcaaa tggttacgag agccttcata tcactgtttt
    27121 ggggcctcaa aacaaatggg tggaaattca gattcgcact acgcgtatgg acgaaatagc
    27181 cgaaaaagga ttggcagctc attggaaata caagggaata aaaggtgaaa gcggcatgga
    27241 cgaatggctg aaaaatattc gcgatatttt ggaaaatccc gacttgaatg ccgtagattt
    27301 tatggatgaa ttcaagttga atctttacga tcaggaagtt ttcgttttta ctccggcggg
    27361 agatttgcac aagttgccaa aaggcgctac ggtactggat ttcgcttatc tgattcacag
    27421 caatttggga agtaagtgca ttggagccaa aatcaacaag gaaagaaatg tgccgataaa
    27481 atatgtgctg aaaaacggag atcaggtgga gatcctcaca tctccctccc aaaagccaaa
    27541 tccttcgtgg ctgaacatag tggtaacttc aaaggcgaaa tcgaaaattc gtcaggcgct
    27601 gaaagatata gagtttaaag atgccgaaat tggtcgggaa attcttttgc ggcgtttcaa
    27661 aaactggaaa attgagttgg aagaaggaaa gctttcgcga ttggccaaga aaaaaggtta
    27721 taaaaccgtc agcgattttt atcaggccat tgcacatgaa aaaatcgaca tgctgctcat
    27781 tcgcgatgcc tatctcgacc tcgaccggca tgaaacggga gcagaacagg ctgaacctcg
    27841 gagtgccgaa actttttcaa aagaaactcc tgccgaagaa ctttcttcaa aagaagatgt
    27901 tctgattatc ggccaaaatc tgaaaaatgt ggattacaca cttgccaaat gttgtcaacc
    27961 catttacgga gacgacattt ttgggtttgt atccgttggc ggaggcatca aaattcatcg
    28021 aaccacttgc cccaatgctc ctcagttgat ttcaaaatac ggttatcgaa ttatcaaggc
    28081 tcaatgggcc ggacgctcag gaggaactca gtatccgatt acactttata ttgtcgggaa
    28141 agatgacatc ggtattgtaa ccaacattac ttcacttatt tcgaaagaga aaaatatttt
    28201 gttgcgttcc atatcggttg attccaatgc cggtgttttt cagggaaacc ttacaataat
    28261 ggtaaacgat gccaatgatt tggacagtat cataaaaaaa ataaaacagg taaaaggtgt
    28321 tctgaatatt tcaagaacca aaagtttaac ccaaaacaac cagtaaaatt ttataaacaa
    28381 tgttttcagg aataattgaa gaagctgccc cggtagtagc tttaaaaaaa gaagccggca
    28441 atttgcacat taccatgact tgctcgttcg taaacgaatt gtcggtcgat cagagtgttt
    28501 cgcacaatgg cgtatgcctc accgttgttt ccaaaactcc cgatacctac accgtaacgg
    28561 ctatccgtga aacgttggaa aaaagcaatt tgggattgct ccaagaaggg agtaaggtga
    28621 atctggaaag gagtttgcta ctcaccgaac gtctcgatgg acatattgtt cagggacatg
    28681 tcgatacaac agcagtttgt accgatattt ctgaagctga tggcagctgg tattttactt
    28741 ttcaatatga tgtggacagg gaattggcaa aaagaggtta tgttaccgtt gacaaaggtt
    28801 cagtggcggt gaatggagtc agtcttaccg tgtgcaaccc gacggaaaat acctttcagg
    28861 ttgcgataat cccctatact tacgaacaca ccaattttca tcagattgtc gtggggacga
    28921 cagtcaatat tgagtttgat attattggaa aatatatcag taggttgatg aaattgtaaa
    28981 taattccctg attttttttg ttttatcaaa ctaaaatcga tggcaattgt tatataacat
    29041 tgcaaaatcc atttagaatt tggtaatgga aaatttcagg aaacagataa aaatttcatt
    29101 tttcaatccc attattcatt ttcttccgct tctgctgttt ttggtaacag ctatgtatgc
    29161 cggaagaacg gtggcgaatt atgtttccat tttcgtcctt cttgtgtcgg cggtatatgt
    29221 ttttattgct tataatcaga tatttatttg gtttttcctg tcgtgtgtca tctttttggc
    29281 ggtcaatatt gttggcagta taattcgttt gccgataatt tttctgagtt ttcgaccttt
    29341 gttggatgaa gcgttgacat tggtattttt gggaggaatg atattgctgc aaaaacccat
    29401 aaaaaaagga attatggcac tcattcccaa aactttgcca atgaataaca accttgatga
    29461 atatttcggt tttatccgaa ctattttctt gataattttg ggatatgttt tggctaccgg
    29521 ttttttctat gcttttccta cgcagcggga aacggcttat ttgcaactgg tgcaggcaat
    29581 ttatgtcgga attcttttct ttgttttggt tttcgaatcg cttcgggttc aaattattcg
    29641 tgcacgcttg ctgaaagaag aatggttgcc gatagtcaat tcgcagggaa aaattattgg
    29701 cagcgtgcaa catcaagcca gtttgggaga aaagcgaaaa ttcatgcatc ccattattcg
    29761 tgtacatatt attcaggatg gcatgatttt gctcagaaag cttcctgctg acgatttgtc
    29821 ctatccttta cattgggata cagcaatttc aaatcatatc cgcatgggag aaacggttga
    29881 agactgcatc aaaaggacgg ctcaagagct atatggtttg accgagttaa aatctttttt
    29941 cctttccaat tatattaccc acacagattt tgaacagcat tatgcttttc tttttattgc
    30001 ctgcaaaatg aataaaatag acctccagtc ggaaaaagtg aaacaggcaa aatggtggac
    30061 gctgcaacaa attgaagaaa atttgcagga aggaattttt accgaaaact ttctgataga
    30121 atttgatttg ttgaaaaaga gtggagtttt ggagaataat atttgcgatt gtgcgtgcaa
    30181 attgaaagaa accattttca aagctcctca tctgtttcgg taaaagcaaa gcataaataa
    30241 cttaatgatg agattatatt ttattttgct cctaatagca tttgtttcct gtggtgggca
    30301 ttccaactcg gaatttaagc tgcaaaattc ctttcaaatt aagctgcagg attcgatttg
    30361 tatcaatgct aaagattgtt tttattttag taccgacagt aacctttatc ggatctttat
    30421 gtatttttca aatgcagaat tgaaagaaaa aaaaataatt gataccgtag attttagccc
    30481 atataaatca aagatacata gctttcaatc ggaaaataat gagtcttatg ttgttttatg
    30541 ggaaacagaa tacgaatttt atcctttaat ttatgtctac tttattacag caggtaaaat
    30601 agtaaaaatt ggagaacttt taatttcttt accctgtcag tcgtgtgaga gtttcgaata
    30661 tccaataaaa gatattcgaa tgcttcaaaa gggaatgaat attgaaatct catttttaaa
    30721 agatgtaaat tataagccaa gtaacggtaa tgaatggcat ttgtataaag ccggtatgtt
    30781 aaaatgcatt tttaatgctg aaacaaacaa attaaaatag atctcatcat tgaattagtg
    30841 ttaagttaga tgacaaaaac aatttatttc acttaaagat aaacctatat tattgattat
    30901 ctatgtttta cattatataa agatattttg tctctcgctg gcttcagtga tttaccactg
    30961 ccaaaatccc ctgatttgta aggttttgga gagtggaagg agtgttcttg ttattaaggt
    31021 ttgagcaaaa taaggttgaa ggaaatac
//
LOCUS       DAOTAX010000019        30634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1229_length_30634_cov_33.928284, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000019 DAOTAX010000000
VERSION     DAOTAX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30634
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_30634_cov_33.928284"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..858
                     /locus_tag="PLM04_03565"
     CDS             478..858
                     /locus_tag="PLM04_03565"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HON02189.1"
                     /translation="MNLNKILAGFIFAGRGIRTAFISERNLKIQTCFVVLVLLFGWLL
                     HIRIVEWLICLLCFGLVIGMELINTSLEKLVDLVSPEYHPLAEKVKDIAAGAVLFDSI
                     IAAIIGLIIFVPKIWEYLLRLISS"
     gene            904..1599
                     /locus_tag="PLM04_03570"
     CDS             904..1599
                     /locus_tag="PLM04_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HON02190.1"
                     /translation="MKTNFIVDFINLMYPNLCLICGENLMKNEDYLCMNCLHQIPKTN
                     YHLLPDNPIEKRFWGKAEVFRATSFFYFQKGSLFQKLIHELKYNGNKEIGESLGKYAG
                     ADLISSADFSTVDMIVPVPLHPKKFAKRGYNQSEWIGKGLSSVMQKPQITNNLIRVKE
                     NTTQTKKSVYERFENTEGIFDLKDGLLFSGKHILLIDDVLTTGSTLEACVHALEKTEN
                     KNIKISIFTLAVA"
     gene            1614..2144
                     /locus_tag="PLM04_03575"
     CDS             1614..2144
                     /locus_tag="PLM04_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HON02191.1"
                     /translation="MKNRIEIVKGDITKLQADAIVNAANRTLLGGGGVDGAIHQAAGH
                     ELLEECKKLGGCNTGEAKITKGYHLPSKYVIHTVGPVWRGGDKNEPELLANCYRNSLL
                     LAKKYQIHSLAFPSISTGIYGYPIEKASVIALQEIAKFLKTEEIPEKVIIVCYSDHDY
                     QIYLQNFNEILTSEKN"
     gene            2332..3288
                     /gene="ftsY"
                     /locus_tag="PLM04_03580"
     CDS             2332..3288
                     /gene="ftsY"
                     /locus_tag="PLM04_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HON02192.1"
                     /translation="MGLINLFNKNKDKNENLEQGLQKTRESVFSKLSRAVIGKSKVDD
                     EVLDNLEEVLISSDVGVETTLRIIERIEKRVARDKYVNTNELNNILKEEIAALLSENN
                     TGFFTDFNAELPAKPYVIMVVGVNGVGKTTTIGKLAYQFKQAGKKVYLGAADTFRAAA
                     IEQLVVWGQRAGVPVIKQQMGSDPASVAFDTLSSAKANDADVVIIDTAGRLHNKVNLM
                     NELSKIKNVMKKIIPDAPHEVLLVLDASTGQNAFEQAKQFTKATDVNALAITKLDGTA
                     KGGVVIGISDQFKIPVKYIGLGEGVDNLQLFDKNAFVESLFE"
     gene            3345..4634
                     /gene="rimO"
                     /locus_tag="PLM04_03585"
     CDS             3345..4634
                     /gene="rimO"
                     /locus_tag="PLM04_03585"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HON02193.1"
                     /translation="MKKTVDVITLGCSKNLVDSELLMRQFEALGYQVRHDPQNPNGEI
                     VIINTCGFIGDAKEESIRTILQFAERRKKNKIDKLLVMGCLSQRYQNELQAEIPEVDK
                     FYGKFNFPEILTDLGQDFRADLRWERTLTTPSHYAYVKISEGCNRTCSYCAIPLITGR
                     HRSRPMEEIINEVQSLTSKGVKEFQLIAQDLSYYGADLYKSLKLPELVERLSDIPGVE
                     WIRLHYAYPAQFPYDLLKVMRERDNVCKYLDIALQHISDKMLKVMRRNFTKQETIELI
                     ETIRKEVPGIHIRTTMLLGHPGETEDDVEELKKFIKQTRFERLGAFAFSNEEGTYAFD
                     NYLDNIPEEEKLKRVDEIMAIQQKISAEINQTKIGKRLKVIIDRQEGENFIGRTEFDS
                     PEVDPEVIISDKVQGIEIGQFYLVEIVDADEYDLYAK"
     gene            4656..4946
                     /locus_tag="PLM04_03590"
     CDS             4656..4946
                     /locus_tag="PLM04_03590"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HON02194.1"
                     /translation="MNYKDFIQKVSTQTALTKEETEKLLETAINVFLEQLTENKSIGI
                     QGFGTFELRKKEERIIVHPATKKRLLMPPKLTVHFKSSNVLKEKLNNQLKNE"
     gene            4939..6228
                     /locus_tag="PLM04_03595"
     CDS             4939..6228
                     /locus_tag="PLM04_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HON02195.1"
                     /translation="MNNEKLSLQEFIDLFAQKANLKNKTAEDFIKNIVLVIEEALLKG
                     ESVKIKNFGVFKPQWIEPRKSVDVNSGKEIILPGSYKIGFVPDENLQNIVNEPFSYLE
                     AVELDIIDSSEKKEEETLEPLKIFSEQADEIKSIIAEIQSMSAHPDVKISESKEKQEK
                     IIPTENEDKKSEIFLEPEINNTDIVENESLKHLNDIENITSENVSEEKQEEKNNEIFD
                     KKSNHQKEDDFLEEFDEDTKSEPIGIPFQAYVPSEEYEFHENTEKKRYSWVWIPVVLM
                     LLLGGAGALYYFYPPVKNFVNLKMTKKSPILTERSLPSPAALTDTIQQQRQPEKVDSL
                     QILFDTPRAYSEFIAKERIRSGSRLARMAKRYYGASEFWVYIYEANRDKIPSPNDVPV
                     GTLIKIPKLDPRLIDVSNPRCMEKAKELSDLYLSLSK"
     gene            complement(6253..7587)
                     /gene="argH"
                     /locus_tag="PLM04_03600"
     CDS             complement(6253..7587)
                     /gene="argH"
                     /locus_tag="PLM04_03600"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HON02196.1"
                     /translation="MAKLWEKDLPLNKKIESFTIGRDKELDLFLAKYDVLGSIAHARM
                     LNTVGLLTAEETKLLIAELKKIYCSIENGEFKIEEGVEDVHSEVEFLLTDALGDTGKK
                     IHSGRSRNDQILLDLKLFTRAEIERTVRAVSSLFEVCQAQSERYKDILMPGYTHLQIA
                     MPSSFGLWFGAYAESLADDLQLLLAAWKITNRNPLGSAAGYGSSFPLNRQMTTDLLGF
                     DSMNYNVVYAQMGRGKMERIVSTALSGVAATLSKLAFDACLYNSQNFGFIKLPDEFTT
                     GSSIMPHKKNPDVFELIRAKCNKIQSVPQQILLITNNLPSGYFRDLQIVKEVFIPAFN
                     EMIDCIEMTKLMLEKIEVNVHILDDPRYDYLFSVEEVNQLATNGMPFRDAYKKVASEI
                     QNNSFRPQKNLHHTLEGSIGNLCNDQIKAYKEAILAQFTFEKVHQAEKALIE"
     gene            complement(7635..8060)
                     /locus_tag="PLM04_03605"
     CDS             complement(7635..8060)
                     /locus_tag="PLM04_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HON02197.1"
                     /translation="MVTYESEIKKINSNAENIFNKFVDLQNFNKLINNKLSEDQIKDI
                     EFSEDSCSFSIEGFGRIGFRIIEKEPFKTIKFELRELPVQANAWIQLVEKDENDTRMK
                     LTFKAEIPAMLKIMVDKKLKEGVNLAAEAIANAFNNSNL"
     gene            complement(8077..8715)
                     /gene="pyrE"
                     /locus_tag="PLM04_03610"
     CDS             complement(8077..8715)
                     /gene="pyrE"
                     /locus_tag="PLM04_03610"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HON02198.1"
                     /translation="MKTLEKNIAEKLLKIKAIKLQPNNPFIWASGWKSPIYCDNRKTL
                     SYPSIRNYIKIELTRLIMELFPDVEVIAGVATGAIAQGVLVADALNLPFIYIRPTPKD
                     HGLENLIEGDLRPHQKVVIVEDLVSTGGSSLKAVEAIRNNGSQVLGMISIFNYGFPVA
                     EEKFKQAKVKHISLCNYKAILDQALTTNYITQEDMKTLQEWHKDPSAWGLES"
     gene            complement(8823..9188)
                     /locus_tag="PLM04_03615"
     CDS             complement(8823..9188)
                     /locus_tag="PLM04_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HON02199.1"
                     /translation="MSAHNDIGNAGEERAQAYLREKGYKIRHTNWHAQKFELDIVAEK
                     DGLLIIVEVKTRSTENFEHPEDAVTPKKIRNIVQAAQEYIFQNNWEGETRFDIISVIP
                     SGQTFHIEHIEDAFLPPIN"
     gene            complement(9279..9977)
                     /locus_tag="PLM04_03620"
     CDS             complement(9279..9977)
                     /locus_tag="PLM04_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HON02200.1"
                     /translation="MKSRNVFMIYVMSAAIIFSGCKSMSKTASGALIGSGAGAAVGAG
                     IGALIGKDAKSAGIGAAIGTAVGATTGAIIGNKMDKAAQEAAAIEGAKVETITDINGL
                     PAVKVTFESGILFETNKSTLNAASKDALTKFAQIMIQNPTMDIAIIGHTDNTGSLEVN
                     QKLSQERAQAVANFLKSQGVPASQIKDVIGKNFSEPVADNSTAAGRAANRRVEVYMYA
                     SEQMIKEAQQQSQK"
     gene            complement(10026..10098)
                     /locus_tag="PLM04_03625"
     tRNA            complement(10026..10098)
                     /locus_tag="PLM04_03625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10063..10065),aa:Lys,seq:ctt)
     gene            10369..11811
                     /locus_tag="PLM04_03630"
     CDS             10369..11811
                     /locus_tag="PLM04_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HON02201.1"
                     /translation="MNELESLFQQFTGEKVLLKEELSASGSHRRYFRLESKHFSIIGV
                     EGTSIEENRAFIEMDKLFHKKGLPVPEFYIQSQDGLYYLQEDLGNTLLFDFIAEGRKT
                     GVFCESEKEMLRKTIRKLPEIQVKGAEELDFSICYPQPEFNERSILWDLNYFKYCFLK
                     ATGIDFQENLLEDDFHRFSKILLHSQSNTFMYRDFQSRNVMIKNDEPYFIDFQGGKKG
                     PLYYDVASFLWQAKAKYSEELREELIKNYLSALAELAPVDEMEFREQLKHFVLFRILQ
                     VLGAYGFRGYYEKKPHFLESIPFAIENLRTILQNEISGYPYLLKVLRQMTELKQFREV
                     SVRKPLTVKIYSFSYKKGIPHDDSGNGGGFVFDCRAVNNPGKYERYAHVTGLDESVKQ
                     FLEEDGEILSLLDNAYKLVDASVKRYMDRGFTNLMVCFGCTGGQHRSVYSAQKMAEHI
                     NEKFGIEVQLIHREQNKEQLFPAKKLSYSK"
     gene            11844..12611
                     /locus_tag="PLM04_03635"
     CDS             11844..12611
                     /locus_tag="PLM04_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HON02202.1"
                     /translation="MKALILAAGLGTRLKPITNHIPKALVTIEGKTLLEHQILKLKSA
                     GFNEIIVNVYHFADKIIDFLHSKNNFDIRIEISDERNCLLDTGGAVRKAAWFFDDGKP
                     FLVHNVDILSNIDLMDLMKFHLSSDSLATLVVNQRKTSRQLLFDKQMQLKGWFNETTG
                     QTKPQEINLQLYQKFAFSGIQILSPKVFLLMEKWNEVFSIIDFYLSNVDKEKISGYTN
                     NEIKVLDVGKIQSLEEASIFLKNINKNFFDNSDFNEG"
     gene            complement(12591..13655)
                     /gene="aroC"
                     /locus_tag="PLM04_03640"
     CDS             complement(12591..13655)
                     /gene="aroC"
                     /locus_tag="PLM04_03640"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007135861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HON02203.1"
                     /translation="MSNTIGKFFTFTSFGESHGKAIGGVIDGCPPGIEIDEAFIQNEL
                     NRRRPGQSNISTPRQEEDKVEFLSGIFEGKSTGAPIAFVIWNENQRSADYNHLKDVFR
                     PSHADFTYQQKYGIRDYRGGGRSSARETAARVAAGAIAKLALRKMGIQINAFTSQVGH
                     IAMSKTYEDVDFSLIETNSIRCPEPETAEKMIAYIQQLKEEGDSIGGIITCVVKGVPI
                     GWGEPIFDKLQSRLAQAMLSINATHGFDYGKGFEGVALRGSQMNDEFVKDGDRITTRT
                     NNSGGIQGGISNGQDIYFRVLFKPVATISKKQNTVDIHAQEVELEARGRHDPCVLPRA
                     IPIVEAMTALTLMDLSLIKI"
     gene            complement(13742..14326)
                     /locus_tag="PLM04_03645"
     CDS             complement(13742..14326)
                     /locus_tag="PLM04_03645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON02204.1"
                     /translation="MRIEENKSVSAKYDLFVDGENQGELELMERATEERPLSFIYGVG
                     MMLPKFEENLYGLQAGDSFEFTINDEDAYGPYDEEAVIDLDKKIFEVDGKIDENVVFE
                     GNVVPLMDTDGNRFNAQIVTVTDELVTVDLNHPLAGQNLHFKGQVLEVRDASEAELAA
                     LMNNSCSCGGNCSSEEGCDCESDSCEDPNCSCNK"
     gene            complement(14444..17224)
                     /locus_tag="PLM04_03650"
     CDS             complement(14444..17224)
                     /locus_tag="PLM04_03650"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HON02205.1"
                     /translation="MHHNFKFHNSLKCKLFLLITGLNISFCLQAKNLNPSPSDTLQAL
                     PDSVLTTNESLTVSVPRENPNKIDAEIIYSASDSIVFYGNGTGFLHGKGDVKYKNITL
                     QADFIRVKMDSSLIYARGTTDSLGNKIGEPVFSEGEATYYSKELTYNLQSKKGYIRNA
                     VTQQGEGYVISDKTKKLDDNILLIADGKYTTCDKHDNPDFYLYLTKGKIKPGDYIVTG
                     PAYLVLADVPLPIAVPFGFFPFTDKYSSGILMPNYADELNRGFGLTNGGYYFALSDYA
                     DLELLGDIYTKGTWALTANSRYIKKYKYNGNFSFSYRVDVTGEKDLPDYSKATNMNIR
                     WTHSQDQKANPYFTFTSSVNFSTSGYNRSNINSYFNPNLNSENTKGSSISFTKRFPSQ
                     SNLSLSGSMLINQRTKDSTISLTLPTLNIAVSRFSPFKRKNAVGNERWYEKIYMSYSG
                     TFANSIDTKESLLLSSSLSKDWRNGMRHSIPVSATFNLFKYINISPSFNYTERWYLRS
                     VDKNWDSDKQKEITDTVSGFNRVYDFNMGVSASTKLYGFFIPIRSIFGDKIDRIRHVF
                     TPTIGFGYAPDFGDPRWGYYDSYKKTTKDPSNPASMIEEEVVYSHYQGSLYGYPGSGK
                     SGNINFSFANNIEMKLRNDNDTTGKVPFKKVSLIDNFSVSGGYNLAADSMQWSNFSTN
                     LRIKFSDKYSLNLSGAFDPYMYGLNQYGNPVRINKLRWNYHKFPKFLGTNASYSLALN
                     NDTFKKLFSRGDNKKTDVKNNTSSKENLDNPDDESITTSPEINQLGLSEKSNQEIQTD
                     ANGYQKVTIPWNINISYSVRYANTNVFDKEKMEYKMGFTHNLSFSGDISLTPKWRISS
                     STSYDFVAKQFTYTSINVSRDLHCWNMSASFVPFGPFQSYYFRIGVNSSMLRDLKYEK
                     RSGYGSTPITWY"
     gene            17464..18690
                     /locus_tag="PLM04_03655"
     CDS             17464..18690
                     /locus_tag="PLM04_03655"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HON02206.1"
                     /translation="MKIPFQKYLLLPYGFLILFLSIFASSVAAQEKKFVVVIDAGHGG
                     HDPGAMGKISREKDINLAVAKELAKLMENNKELKVVLTRKTDKYLTLQERADIANENH
                     ADLFICIHTNSSKSSSAYGAETYTLGLAKTNANLEVAMRENSVILLEENYQTKYQGFD
                     PTSVDSYIMFEFMQDKYLDKSIEFASLVQQHLDRNGRLDRGVRQAGFWVLHRSACPST
                     LIELGFISNPEEELFLSSEYGQKKMAQAIYTAFLDFKKEHDKRNGKLNSTSVSNQAPS
                     KKSENIVSESQNNYSTPIQQAEIENINTANITNQEKQTENILPSTQQPVFKLQILASK
                     KKLSPQANELKGLKSVDYYIENELYKYTVGAETDYNKIVQLKNNLSAKFPQAFIVAFV
                     NGKKVDVKEALRLTGK"
     gene            18808..19719
                     /locus_tag="PLM04_03660"
     CDS             18808..19719
                     /locus_tag="PLM04_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HON02207.1"
                     /translation="MKKNFFNKEVKIGLMVFVAIFLLYFGLNFLKGIDIFTSTRSYYT
                     TYENLDGLVASSPVFIKGYKVGQVDEIQYDFSKEKPFLVKITIEKKINLPKNTKIQLF
                     DNGLMGGKAIQLIFAPYASSQNFCNNGDTLQSEIKPGLMADLSSNLLPKIESISTQTD
                     SLLRSIRSITEGEKLNNSLTSIEKTTADLAETSAQMKNLMKYQLPSIMSNLNGASSDF
                     KSVTSNLKEINFASTMNNVDQTVSNLNLLTNKINNGEGSLGMLLTDQQFYLNLANTSS
                     SANQLLIDLKNNPKRYVHFSLFGSKTK"
     gene            20130..21512
                     /gene="dnaA"
                     /locus_tag="PLM04_03665"
     CDS             20130..21512
                     /gene="dnaA"
                     /locus_tag="PLM04_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HON02208.1"
                     /translation="MHEQLWERCLKVIRDNINEASYKTWFEPIIPLQYENKRFVVQVP
                     SQFFVEYIEEKYIDLLSRTIYREIGSGTQLEYRVLIDQTNGKGTQIPSTAEQPKLKSS
                     NTTQGTKNPYDKSSLPNIDPQLNMKYNFNTLIEGKSNKLARTAGVTIGAEPGKTAFNP
                     LFVYGESGVGKTHLANAIGVKAKQMHPDKRVLYLSANTFQIQYTDAVRNNTVNDFVNF
                     YQTIEVLIIDDIQEFSGKTATQNTFFHIFNHLHQSGKQLILTSDRSPLLMAGLEQRLL
                     TRFKWGLSAEIEKPDFELRKEILKNKIYCDGLDIPEDVVNFIAQNVTDNIRDLEGVLI
                     SLLAHSTLTDMPIDLQLAEKVVSRIVNIPKKINTVEKIRDTVCDYFSIPVDAIFTKSR
                     KREVVQARQIAMYLSKQFTKNSLSSIGKAIGERDHATVLHACKIISDLMDTDKKFRSN
                     VKAIEEILRN"
     gene            21659..22219
                     /locus_tag="PLM04_03670"
     CDS             21659..22219
                     /locus_tag="PLM04_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02209.1"
                     /translation="MVFRFTILSDEVDNFLRVIDIDAEATFFDLHNAILDAVNFKKDQ
                     ITSFFLCSDDWEKEQEITLIEMDSSSEYDNLVMDKVKLEELLTDEKQKLMYVFDMIAD
                     RAFFIELSEIIPRKKLEKPVCIKSIGNPPEQTLSENDVNQAPKLNVEENFYGDEDFEL
                     DELDEEGFSDFNFDDDSNLFNDDTNF"
     gene            22286..23188
                     /gene="miaA"
                     /locus_tag="PLM04_03675"
     CDS             22286..23188
                     /gene="miaA"
                     /locus_tag="PLM04_03675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HON02210.1"
                     /translation="MQSTLIVILGPTGVGKTEISLQVAQNLQTPIISADSRQIYKELK
                     IGTAAPTSEQLSTVKHYFIGSHSIFENFSAGQFEQEALALLEKHFATNHIALMVGGSM
                     LYIDAVCKGLDNIPTVDTTTRNFWKKQYENQGLEYIRQELKKLDPVYYQQVDPNNYKR
                     ILHALEICSITGNPFSALRTGVHKKRAFDIVKIGLNRPRHELYERINARVDQMMEEGL
                     LEEAKQFYAFKNLNPLNTVGYKELFEFMDGKITLKQAVDKIKQNTRHYAKRQLTWFNH
                     DKDITWFHPDEREKIIRFLQNRLS"
     gene            23199..>23658
                     /locus_tag="PLM04_03680"
     CDS             23199..>23658
                     /locus_tag="PLM04_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HON02211.1"
                     /translation="MNIKYNYSLLSHNTFGIDVKAEEFIEFYTVDEALDLSKTLNHKK
                     WFVLGQGSNLLFLNDFKGVVLHSGIRQMEKIKEDNQFVYFEVGSGVVWDQFVEFCVKN
                     NWSGIENLSLIPGEVGAAAVQNIGAYGMEVQEVIEKVKAIDTATSQLMEFS"
     assembly_gap    23659..23758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23759..24361
                     /locus_tag="PLM04_03685"
     CDS             <23759..24361
                     /locus_tag="PLM04_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HON02212.1"
                     /translation="IEKVKAIDTATSQLMEFSKENCQYGYRQSIFKNQYKGRYILTSV
                     VFRLSKKPNFRLHYQHLEEEVRKRGNINLQTIRETIIQIRSQKLPDFRTMGNAGSFFV
                     NPVVSKVHFQRLQSEYPEIPHYYVSETEEKIPAAWLIEQCGWKGKQVGKVAVYAKQPL
                     VIVNCGGASGSEIKQLAEEIQLSVKNKFDIQLQPEVNYIE"
     gene            complement(24560..25528)
                     /gene="floA"
                     /locus_tag="PLM04_03690"
     CDS             complement(24560..25528)
                     /gene="floA"
                     /locus_tag="PLM04_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213154.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HON02213.1"
                     /translation="MGIEIVLLIVLVVVLLLFFYYVPFLLWLSAKVSGVSISLVQLFL
                     MRIRKVPPHSIVQCLIEAHKADLHEIKRDELEAHYLAGGHIERVVHALVSASKANIDL
                     TFKMATAIDLAGRDVFQAVQMSVNPKVIDTPPVAAVAKDGIQLIAKARVTVRANIKQL
                     VGGAGEETILARVGEGIVSSIGSAESHKAVLENPDSISKLVLKKGLDAGTAFEILSID
                     IADIDIGKNIGAQLQMDQAEADKNIAQAKAEERRAMAVALEQEMRAKSQEMRAKVIEA
                     EAEVPKAMAEALRNGNLGVMDYYRLKNIEADTAMRENIGKPSDKTK"
     gene            complement(25543..26034)
                     /locus_tag="PLM04_03695"
     CDS             complement(25543..26034)
                     /locus_tag="PLM04_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HON02214.1"
                     /translation="MDIAIAIILILVGVFFFLVEFFLVPGISFAGIAGFLFVGAGIYY
                     SYSQLGTTAGNVSIAGSVVLLAVAVWIFLRRKTLERISLKAKIDSSIGTVEELNIHAG
                     DTGIALSRLAPMGKVKVNGQTIEAKSVDEFIDEGTPILVKEVFKTNVLVERISENSNN
                     EHA"
     gene            complement(26082..27473)
                     /locus_tag="PLM04_03700"
     CDS             complement(26082..27473)
                     /locus_tag="PLM04_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02215.1"
                     /translation="MRSKISLITVLLFFCVAILGQTAAEADRLFQQNQFQKARDAYKI
                     LLEKRPKDALYNYRYARCCYELKDDENAIKHFELSGNRFPLTNKYLGELYMKTYRFSQ
                     SVAAYEEYLKTINETDKEFLTIQKAKKKAELAEKMIQRVDDIQIIDSVVVNKDHFLEF
                     YKFTNDLGSLSATPVKIGKKKDFKITYTTQRQDRKLFSDTVHGQMDIFTSYKLLDEWS
                     KPESVSDIINTKANENYPFLLLDGVTIYFASDGDNSIGGYDLFVSRFRPETNTYFQPE
                     NIGMPYNSIYNDYMMVIDDIHQVGWFATDRFQPEGKVIIFSFIPNTKKTILQSEDSEY
                     IRKAAQLQLFKKAKNFNSDIIPQTSFADEDSVHKIFFVVNDSVIYTDDNQFKTEETRN
                     LWNELEKLNSQLNQKRIQLEQLRMQYSQQEETQNELAPKILTLEKEISEMEDQLTTKT
                     FELRNKENINLMK"
     gene            complement(27549..30245)
                     /locus_tag="PLM04_03705"
     CDS             complement(27549..30245)
                     /locus_tag="PLM04_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HON02216.1"
                     /translation="MKRKNGRILGIIFSILIVLFAVLMILPFAFQGKIFNAVKLQINK
                     MLTAKVDFQNVQLSFIRHFPNASVRINGLKVIGEGDFAADTLVSADKIEAVVNLKSLL
                     SGKGYDIQKINVVNPHIMAHVLADGRANWNIMKDTAEEKTDTAAMKFHLKLKDVFIQH
                     ANIAYLDEQSNMKAIISNLNHHTSGDLTADSSLLSTQTTIDSISFWMNGIPYLLRANA
                     ELNVDIDANINDMKFAFSKNSSRINAIPFSFEGWVKMLDEGFDMDIKLIANKVDLKSI
                     LSMIPAIYSNSFKDLKTGGEVSLNGFVKGQIKDENYPAFDVNLSINNGWFQYPSLPQS
                     VKNIQLAFQIVNPGGSLDSTLIDLSKFSLTLGSNTFNAAAKIASIISDPDIRAKASGR
                     IDLGMVKSFYPLEPGTNLNGLLDLDLNLNGKMSYLENNLYDKFGFQGKLLANNIFLKT
                     SSLPQDISVNRAQVFFNNRYVNLSDFQLKIGKNDLQANGKLENFLAYALKNKTLKGQL
                     NLQSNYLNVNDFLTETTETKNEKMELFVIPQNIDFTLQAFFKQLLYDKMNFSNIQGKL
                     LVQNGEIKFQNLNLEAFGGTLLANGLYSTANVTKPYADINLNIKNVAFNQIFSQVGSF
                     QKFVPFFEKATGNFSTTLSLNTLLNNDMSLNLSSLKANGKFSTSSVALKDVPALSALA
                     SGLKKPELSNPSLKDLAVNFEISNGKLFTKPFDIKLGDIKMNLGGSMGLDQSLDFKGI
                     VQLPDQLNAGRFSNFNVTIGGTYDKPKVKLDLLDTFNEALEETKAKIKEEVATQVDAA
                     KKEVVEKARKEREAALKEAQVQADKIRLEAKKAGDKLIEEATLKGNELVEKAANPIAK
                     KVAEQSARKLVEEARKQADKLNADAEIQANKIIENASKNTNF"
BASE COUNT         9948 a         5700 c         5454 g         9432 t
ORIGIN      
        1 aacagtgtaa cgatagaatt taaaagaaat tttaaaaata tttcctgtaa aaaatctgtt
       61 gaacaaattt gttcaacaga tttttttata tttttgtgct tagtacatga caaaaaattg
      121 tacaaatatt tatattattt attttcagat atttaacaaa ggggctttgc cccgaacccc
      181 acttactttt ttgtcttgaa acaaaaaagt aagcaaaaaa attcaagact gtgcccactt
      241 cactcgaaaa acttacgctc gaaaagctga aatcgtccaa actcattccg acgaaatacg
      301 tcggaactca aacaaggacg atttttaaac gcttttctca cttgtttttc ggctcaccgg
      361 acaaggtcaa cccgaagagc ttggcttata tgttctgttt tacagatata taaaacaaat
      421 gtttcaattt ttgtcatgta ttaaaatttt tgtgaatcag aacacatttt tgttaacatg
      481 aacctgaata aaatacttgc cggatttatt tttgccggaa gaggaattcg tactgcattc
      541 atttcggaaa gaaatttgaa aattcaaacc tgcttcgttg tgttggtctt acttttcgga
      601 tggttgttgc atattcggat cgtcgaatgg ttaatttgtc ttttgtgttt tggactggtc
      661 atcggtatgg aactgataaa tacttcctta gaaaaactgg tcgatttggt ttcacccgaa
      721 tatcatccct tagcggaaaa agtcaaagat attgctgcag gcgccgtatt gtttgattct
      781 attattgcgg caattattgg attgataatt tttgtcccga aaatttggga atatcttctt
      841 cgcttgattt catcttgagt aaagtctaat tttcgtaatg tttttatccg aaatcacagc
      901 cttatgaaaa ccaattttat tgttgacttt atcaacctca tgtatccgaa tctttgcctt
      961 atttgtggag aaaatttgat gaaaaacgaa gattatcttt gcatgaattg cctccatcaa
     1021 attcctaaaa caaattatca tcttttgccc gacaatccta ttgaaaaacg tttctgggga
     1081 aaagccgaag tgttccgggc tacttctttc ttctattttc aaaaaggatc actgtttcaa
     1141 aaacttatac acgaactcaa atacaacggg aacaaggaaa ttggcgaatc gttgggaaaa
     1201 tatgccggag cagatctgat aagttcagcc gatttttcca cagtagatat gattgtaccg
     1261 gtaccattgc atccaaaaaa atttgccaaa agaggttaca accaaagcga gtggattggt
     1321 aaaggtttgt cgagcgttat gcaaaaacca caaatcacca ataatttgat tcgcgtaaaa
     1381 gaaaatacca cccaaaccaa aaaatcggtt tacgaaagat ttgaaaacac tgaaggaatt
     1441 tttgatttga aagatggttt gttgttttcg ggaaaacaca tattgctgat tgatgatgta
     1501 ttaactacag gctcaacatt ggaagcctgc gtccatgcac tcgaaaaaac tgaaaacaaa
     1561 aatatcaaaa tcagcatttt tactttagca gttgcttaaa tttaatcaca attatgaaaa
     1621 acagaataga aatcgtgaag ggcgatatta caaaactgca ggcagatgcc atagtaaatg
     1681 ccgccaatcg aactttgttg ggtggaggag gtgtggacgg tgccattcat caagcggcag
     1741 gacatgaact attggaagaa tgtaaaaagc ttggaggatg caataccggc gaggcaaaaa
     1801 taaccaaagg atatcatctc ccttcaaaat acgttattca taccgttgga cctgtatggc
     1861 gaggcggaga taaaaatgaa ccggaattat tggctaactg ttatagaaac agtttgcttt
     1921 tagcaaaaaa ataccaaatt cactctttag cttttccttc tattagtaca ggcatttacg
     1981 gctatcccat agaaaaagca agtgtcattg cgttgcagga aattgcaaaa ttcttaaaaa
     2041 ccgaagaaat acccgagaaa gtcattattg tttgttatag cgaccatgat tatcaaatct
     2101 atctgcaaaa cttcaatgaa atattaactt ccgaaaaaaa ttgattttct cttctctttc
     2161 actccatttt ccagcctttt ctgacaataa aaattgctga acactgcata ttctactaaa
     2221 ttatttgtat ccaattgttt gtatttctgt ctttgtggat atgttgttcc aaaatcggaa
     2281 aactcggaaa aatgttataa ttttgtaaat tgaaatttaa aaagcaaaag aatgggactt
     2341 atcaatttat tcaataaaaa caaggataaa aacgaaaatt tggaacaagg attacaaaag
     2401 accagagaaa gcgttttttc caaattatca cgggcagtaa tcggaaaatc gaaagtggat
     2461 gacgaggttc tcgataacct ggaagaagtt cttatcagtt ccgacgtagg cgttgaaacc
     2521 actttgcgaa tcattgaaag aatagagaaa cgtgtggctc gtgataaata tgtgaatacc
     2581 aatgaactaa acaacatttt gaaagaagaa attgcagcac ttctttctga aaataatacg
     2641 ggatttttta ccgattttaa tgccgaattg cccgcaaaac cttatgtgat catggttgtg
     2701 ggcgtaaatg gagtggggaa aaccactact atcggaaaac ttgcctatca gtttaagcag
     2761 gctggtaaaa aagtatattt gggagctgcc gatacttttc gtgctgctgc cattgaacag
     2821 ttggtagttt ggggacagcg tgccggcgtt ccggttatca agcagcaaat gggttccgac
     2881 ccggcttctg tggcttttga tacactttcg tcggccaaag ccaacgatgc cgatgtggtc
     2941 attatcgaca cggcaggacg tttgcacaat aaagtgaatt taatgaacga gctgtcaaaa
     3001 ataaaaaacg taatgaaaaa aattattccc gacgctcctc atgaagtatt gctggttctt
     3061 gatgcttcta ccggtcaaaa tgcttttgaa caggcaaaac agtttacaaa agcaaccgat
     3121 gtcaatgcac tggctataac aaaacttgat ggcactgcca aaggtggcgt tgtaattgga
     3181 atttccgatc agtttaaaat cccggtgaaa tacattggct tgggtgaagg agtcgacaac
     3241 ctgcaattgt ttgataaaaa tgcttttgtt gaatccttgt tcgagtgatt tttgcgtata
     3301 atccaaactt ttaaaataat gtcgtttacc gattcaattt cagcatgaaa aaaacagttg
     3361 atgtaattac tttgggttgt tctaaaaatc ttgtcgattc cgaattgctg atgcggcagt
     3421 ttgaagcgtt gggttatcag gttcgccacg atccccaaaa tccaaacggt gaaattgtta
     3481 ttatcaatac ttgtggcttt atcggcgatg ccaaagaaga aagcattcgt accattcttc
     3541 agtttgccga aagaagaaaa aaaaataaaa tagataaatt gttggtaatg ggatgtttgt
     3601 ctcaacgtta tcaaaatgaa ttgcaagccg aaatacctga agtggacaaa ttttacggaa
     3661 aatttaattt tcccgaaata ctgaccgatt tgggacagga ttttagagcc gatttacgtt
     3721 gggaacgtac cctgacaaca ccctcgcact atgcttatgt taaaatttcg gaaggatgca
     3781 atcgcacttg ttcctactgc gccattcctt tgatcaccgg acgacatcgt tcacgtccaa
     3841 tggaagaaat cattaatgaa gttcagagtc tgacaagcaa aggggttaaa gaatttcagc
     3901 tgattgcaca ggatttaagt tattatggag ctgacctcta taaatcgtta aagttaccgg
     3961 aactggttga acggctgtcc gatattccgg gagttgaatg gattagactg cactacgcat
     4021 atcctgcaca atttccttac gatttgctca aagtgatgcg tgaacgcgac aatgtttgta
     4081 aatatctgga tattgcactt caacatatca gcgacaaaat gctgaaagta atgcgaagaa
     4141 atttcaccaa acaagaaact atagaactga tagaaacaat aagaaaggaa gttcccggca
     4201 tccatattcg tacaaccatg ctgttgggac atcccggaga aacggaagat gatgtggaag
     4261 aattgaaaaa attcattaaa caaacccgat tcgaaagatt gggtgccttt gctttttcca
     4321 atgaagaagg aacgtatgcc tttgataatt atctggacaa tatacctgaa gaggaaaaac
     4381 taaaaagagt tgatgaaata atggctattc agcaaaaaat ttcagccgaa atcaatcaaa
     4441 ccaaaatcgg caaaaggctg aaagttatca ttgaccgaca ggaaggtgaa aactttatcg
     4501 gtcgtacaga atttgattcg ccggaagttg atccggaagt aataatttcg gacaaggtac
     4561 agggcataga aattggccaa ttctatctgg tggaaattgt cgatgccgat gaatatgatt
     4621 tgtatgccaa ataatcatct gaaaaaacta aaaacatgaa ttacaaggat tttattcaga
     4681 aagtttccac tcaaactgct cttacaaagg aagagacaga gaaattgctc gaaaccgcaa
     4741 taaatgtatt tttagaacag ttgacagaaa acaaaagtat tggtattcaa ggatttggaa
     4801 cttttgagct tcgaaaaaaa gaagaacgca tcattgtaca tccggcaaca aaaaagcgat
     4861 tgctcatgcc cccaaaactc acggttcact ttaaatcaag caatgtattg aaagaaaaac
     4921 tcaacaacca gctgaaaaat gaataacgaa aaattaagtt tgcaggaatt tatcgatttg
     4981 tttgcacaaa aggcaaattt aaaaaataaa acagccgaag acttcataaa aaatatcgtt
     5041 ttggtgatag aagaagcttt gctgaaagga gaatcggtaa aaattaaaaa ttttggcgtt
     5101 ttcaaaccac agtggatcga accccgtaaa agtgttgatg tcaatagcgg aaaagaaatt
     5161 attttgccgg ggagctataa aattggattc gttcctgacg aaaatttaca aaacattgta
     5221 aatgagccat tttcttactt ggaagcagtg gaacttgaca ttatcgattc ttctgaaaaa
     5281 aaagaagaag aaacactcga acccttaaaa atatttagcg agcaggctga tgaaattaaa
     5341 agcattatcg ctgaaattca gtccatgtct gcacatccgg acgtaaaaat cagcgaaagc
     5401 aaagaaaaac aggagaaaat aattccaaca gaaaacgagg acaaaaaatc agaaatattc
     5461 ttagagcctg aaataaataa cactgatatt gttgaaaatg aatcactaaa acatcttaat
     5521 gacatagaga atatcacctc tgaaaatgtt tctgaagaaa agcaggaaga aaagaacaat
     5581 gaaatttttg ataagaaaag caatcatcag aaagaagacg attttttgga agaatttgat
     5641 gaagatacaa aatcagaacc tatcggaatt ccttttcaag cttatgtgcc atcggaggaa
     5701 tacgaatttc atgaaaatac tgaaaaaaaa cgttattcat gggtttggat tcccgttgtt
     5761 ctgatgcttt tattgggtgg agcaggtgca ttgtattatt tttatcctcc ggtgaagaat
     5821 tttgtaaatt taaaaatgac aaaaaagtcg ccaattttga ctgaaaggag tctcccctct
     5881 cccgccgctc taacggatac gattcaacaa caaagacagc cggaaaaagt ggacagtttg
     5941 cagattttat tcgatacgcc tcgagcttat tccgaattta ttgctaaaga acgcattcgc
     6001 agtggaagca gattggctcg gatggccaaa cgttattatg gagcttcgga attttgggtg
     6061 tatatttatg aagccaacag agataaaatt ccttctccca atgatgttcc ggtaggaacg
     6121 ctgataaaaa ttcccaaact ggatccacga ttaatcgatg tttccaatcc gcgttgcatg
     6181 gagaaagcca aagaactaag tgatttgtat ctttcacttt ccaaataaaa aagctgaaga
     6241 aaattttctg tattattcaa tcaaagcttt ttcggcctga tgaacttttt cgaaggtaaa
     6301 ttgcgctaaa attgcttcct tgtaagcttt tatctgatcg ttgcacaaat tgccaatgct
     6361 gccttcaagt gtgtgatgaa gatttttttg tggtcggaaa gaattatttt gaatttcgga
     6421 agcaactttt ttataagcat ctcgaaaagg catgccgttt gttgccagtt gattcacttc
     6481 ttccacgctg aaaagataat cgtaacgtgg atcatccaaa atatgaacat taacttcaat
     6541 tttttccagc atcagtttcg tcatttcgat acaatctatc atttcgttga aagccggtat
     6601 aaatacttcc ttaacaattt gtaaatcacg gaaataccct gatggcaaat tattggttat
     6661 caacaaaatt tgttgaggta ctgattgaat tttattgcat tttgctcgaa tcagttcaaa
     6721 cacatccgga ttttttttgt gaggcataat gctggagcca gtcgtaaatt catcgggcaa
     6781 tttaatgaaa ccaaagtttt gagaattata aagacaggca tcgaaagcca gtttggaaag
     6841 agttgcagcc acaccggaca aagctgtaga aacaattctt tccatttttc cacgtcccat
     6901 ttgtgcatat accacgttgt aattcatgct gtcgaatccc aacaaatcgg ttgtcatttg
     6961 tcgattgagc gggaaagaag aaccatagcc ggcagctgat cccagcggat tccgattggt
     7021 tattttccac gcagccagca acagttgcag gtcatcggcc aaactttcgg cataagcgcc
     7081 aaaccacaaa ccaaaagacg aaggcatggc aatttgcaaa tgggtgtaac cgggcattaa
     7141 aatatcctta taacgctcgc tttgcgcctg acaaacttca aaaagtgaag aaacagcacg
     7201 aaccgttctc tcaatttcag cacgtgtaaa caatttcaga tccaacaaaa tctgatcgtt
     7261 acgtgaacgg ccactgtgaa ttttctttcc tgtatctccc aaagcatctg tcagtaaaaa
     7321 ttcaacttcg gaatgcacat cttccactcc ttcttcaatt ttaaattcac cgttctcaat
     7381 ggaacaataa atctttttca attcggcaat caacaacttc gtttcttcag cagtgagtaa
     7441 accaaccgta ttgagcattc gggcatgagc tatcgaaccc aaaacatcgt atttcgccag
     7501 aaataggtct aattccttat ctctgccaat cgtaaacgac tctatttttt tattcaaagg
     7561 caaatccttt tcccagagtt tagccatagt aatgaaacta aatgttttga agttacgaat
     7621 ataaagttaa attgttacaa atttgaattg ttaaatgcat tggcaatagc ttcggcagcc
     7681 agattaactc cttctttcag ctttttatcg accattattt ttaacatagc aggtatttca
     7741 gccttgaatg ttaatttcat gcgtgtatca ttttcatctt tttctaccag ttgaatccac
     7801 gcattggctt gaaccggaag ttcacgcaat tcaaatttta tagttttaaa gggttctttt
     7861 tcaataattc taaaaccaat cctgccaaat ccttctatag aaaaggaaca gctatcttcc
     7921 gaaaattcaa tgtcttttat ttgatcttca gataatttat tgtttatcag tttattgaaa
     7981 ttttgtaaat cgacaaattt attaaagata ttttctgcat ttgaatttat ctttttaatt
     8041 tcactttcgt aagttaccat aaattttttc tttcaattaa gattctaatc cccatgcaga
     8101 aggatccttg tgccattcct gcaatgtctt catatcttcc tgagtaatat agttggtggt
     8161 cagtgcctga tcgagaatgg ctttgtaatt gcacaaggaa atatgcttaa ctttggcctg
     8221 tttgaatttt tcctcagcta cagggaatcc gtaattaaaa atggaaatca ttcccaatac
     8281 ttgcgagcca ttgttacgaa tagcttccac agctttcaaa ctgcttcctc ctgtcgaaac
     8341 aaggtcttct acaatcacta ctttctgatg aggacgtaaa tcaccttcaa tcaggttttc
     8401 caaaccatga tctttcggtg tggggcggat ataaataaat ggcagattca acgcatcggc
     8461 aaccagcact ccctgagcga ttgcgccggt agctacgccg gctataactt ccacatcggg
     8521 aaacaattcc ataatcaaac gtgttaattc gattttgata taatttctga tggaaggata
     8581 cgaaagcgtt ttcctgttat cgcaataaat cggtgatttc cagcctgaag cccaaataaa
     8641 aggattgttg ggctgaagtt ttattgcctt aatctttaaa agtttttcgg caatgttttt
     8701 ttccaaagtt ttcattttgg gatagttata aaattggtaa acaaaaaact gtttaatttt
     8761 tctgcaaagt taagcaaagt atttaagaag ttaaaacaga atttctccaa tcgtttgaga
     8821 aattaattaa tcggaggtag aaaagcatct tctatatgtt cgatatggaa agtttgtccg
     8881 gaaggaataa cagagatgat gtcgaagcgt gtttctcctt cccaattgtt ttggaaaata
     8941 tattcttgag ctgcctgaac aatatttctg atttttttgg gagtaacggc atcttcggga
     9001 tgttcaaaat tttccgttga acgtgtttta acttcaacaa taatcaataa gccatctttt
     9061 tcggctacta tatccagttc aaatttttgc gcatgccaat tggtatgtct gattttatat
     9121 cccttttctc tcaaataagc ttgcgctcgc tcttctcctg cattaccaat atcattatga
     9181 gccgacatat cgaaaaaatt ttgcgaaaat ttggaaattc aaacaaaaaa agccgctcca
     9241 aaatataaga gcagctcttt tattagaaaa taatgaattt atttttgact ctgctgctga
     9301 gcttccttta tcatttgttc gctggcatac atgtacactt ctacgcgacg attggctgct
     9361 ctgccggcag cagtagaatt atcggcaacc ggctcagaga aatttttacc aataacatct
     9421 ttaatttgag aagcaggaac accttgcgat ttcaaaaagt tggccacagc ctgtgctctt
     9481 tcctgagata atttctgatt aacttccagc gaacctgtgt tatcggtatg gccaataatg
     9541 gcaatatcca tagtggggtt ctgaatcatg atctgagcaa attttgttaa agcatctttc
     9601 gaagcagcat ttaaagtact tttgttggtc tcaaataaaa ttcccgattc aaaagttacc
     9661 tttactgcag gcaatccatt gatatcggta atggtttcaa ccttagcacc ttcaatagct
     9721 gcagcttctt gagcagcttt gtccatttta ttcccaataa ttgcacctgt tgtagctcca
     9781 acagcagttc caattgcagc accaattcct gcgcttttag catctttacc tatcaatgcg
     9841 ccaattccgg cacctacggc agcacccgca ccgcttccta tcaaagctcc actggcggtt
     9901 ttactcatcg atttgcatcc tgaaaaaata attgctgcac tcatcacata aatcataaaa
     9961 acatttctgc ttttcatgtc tgtagaattt taaagattat ttttaatatt ttaaaccagg
    10021 aatagtgaca ccggtgggga tcgaacccac gacccacaga ttaagagtct gttgctctac
    10081 cagctgagct acggagtcgt tttatatttc aaaaaagtgt aagaacaaac ttacttctct
    10141 cctttttgta ttgcaaagat agaaagattt ttgaaatttt ttcattttga attttaaaaa
    10201 aatatttagt gcaaattttt cttaatcaac taccaaatat tcaaatattt atagaatttg
    10261 aacaatttac ttcgttttat aatttaacgc gcttaaaaat tcaagcaaag atgtatattt
    10321 ctttttatat ctttgtgtaa cctgcattaa ttaaaaaaaa ttctaaaaat gaatgaatta
    10381 gaatctcttt tccaacaatt taccggagaa aaagttcttc tgaaagaaga attgtcggct
    10441 tccggatcgc acagacgata ttttagactc gagagcaagc acttttctat tatcggagta
    10501 gaaggaactt ccattgaaga aaacagggct tttatagaaa tggataaatt gtttcacaaa
    10561 aaaggattgc cggttccgga gttttacatt caaagtcagg atggtttgta ttatttgcag
    10621 gaggatttgg gtaatacgct gcttttcgat tttatagcag aagggagaaa aacgggtgtg
    10681 ttttgtgaat cggaaaagga aatgcttcgt aaaacaattc gaaaacttcc cgaaattcaa
    10741 gtaaaaggag cggaagaact ggatttctcc atttgctacc cccagccgga atttaatgaa
    10801 cgttccattt tatgggattt aaattatttt aaatattgtt ttctgaaagc aaccggtatc
    10861 gattttcagg aaaatttgct ggaagacgat tttcaccgtt tttccaaaat cttgttgcac
    10921 tcacagtcca atacatttat gtatcgcgac tttcagagcc gaaatgtaat gataaaaaac
    10981 gatgaacctt attttatcga ctttcaaggt gggaaaaaag ggccattgta ttatgatgta
    11041 gcatcgttcc tatggcaggc taaagccaaa tatagtgaag aattgcgcga agaactcata
    11101 aaaaattatc tttcggcatt agcagaactg gcacctgtag atgagatgga gtttagagaa
    11161 caattaaaac attttgttct ttttagaatt ttacaagtgt tgggagctta tggttttcgg
    11221 ggctattatg agaaaaaacc gcattttctg gaaagcattc cctttgccat agaaaattta
    11281 agaaccattc ttcaaaatga aatttcaggt tacccttacc ttttgaaagt gctcagacaa
    11341 atgaccgagt taaagcaatt ccgcgaagtt tcagtaagga agccactgac agttaaaatt
    11401 tacagctttt cctataaaaa aggcattcct catgatgata gcggaaatgg aggcggcttt
    11461 gtatttgatt gcagagcagt caacaatccg ggcaaatacg aacgatatgc tcatgttaca
    11521 ggactcgacg aatcggtgaa acaatttttg gaagaagatg gtgaaattct atctttgctc
    11581 gataatgcct acaagcttgt ggatgcttca gtaaaacgct acatggatcg aggatttacc
    11641 aatttaatgg tttgttttgg atgtaccgga ggacaacatc gatcggttta ttcggcacaa
    11701 aaaatggcag aacatatcaa cgaaaaattt ggcatagaag tacaactaat tcatagagaa
    11761 caaaataaag aacaactttt ccctgcaaaa aaattaagct attccaaata acgttttata
    11821 tttcaaaaaa tctttaattc tgaatgaaag cattgatact ggcagccggt ctgggtacgc
    11881 gacttaaacc aattaccaat catattccta aagcgctggt aacgatagag ggaaaaactt
    11941 tactcgaaca tcaaattttg aaattgaaat cggcaggttt taacgaaata atcgtgaatg
    12001 tttatcattt tgccgataaa attatcgatt ttcttcattc aaagaacaat tttgatattc
    12061 ggatcgaaat ttcagacgaa agaaattgtc tactcgacac aggaggagcg gtacgcaaag
    12121 ctgcatggtt ttttgatgac ggaaagcctt ttttggtaca caatgtcgat attctttcca
    12181 atatagattt aatggacttg atgaaatttc atctaagcag cgacagcttg gcaacattgg
    12241 tggtaaatca acgtaaaacc tcacgtcaat tgctcttcga caaacaaatg cagctgaaag
    12301 ggtggttcaa tgaaacgacc ggacaaacca aaccgcagga aataaattta cagctttatc
    12361 agaaatttgc cttttccgga attcagattt tatcgccaaa agtttttcta ctcatggaaa
    12421 aatggaatga agttttttcc attatcgact tttatttgag taatgtggat aaagaaaaaa
    12481 tttcgggcta caccaataat gaaataaaag tgctggatgt aggaaaaata caaagtttag
    12541 aggaagccag catttttttg aaaaatatta ataaaaactt cttcgataat tcagatttta
    12601 atgagggata aatccatcaa agtaagtgct gtcatggctt ctactatcgg aatagcacgt
    12661 ggcaaaacgc aaggatcatg gcgtccgcga gcttccagtt ccacttcctg agcatgaata
    12721 tctaccgtat tttgcttttt tgaaatggtt gccactggtt tgaaaaggac acgaaaatat
    12781 atatcctgcc cgttacttat tcctccctga atgcctccgg aattattggt tcgcgtagtg
    12841 attctgtcgc catctttcac aaattcgtca ttcatttgcg aacctctgag cgccacacct
    12901 tcaaaacctt ttccgtaatc gaaaccgtga gttgcattta tgcttaacat ggcttgtgcc
    12961 aaacgcgatt gaagtttgtc gaaaataggc tctccccagc caatcggaac acctttgact
    13021 acgcaggtaa tgatgccacc tatagaatca ccttcttcct ttaactgttg gatgtaagct
    13081 atcatctttt cggcagtttc cggttccgga caacgaatag aattggtctc gattagtgaa
    13141 aaatcaacat cttcgtaagt tttgctcatg gcaatatgtc ctacttgcga agtaaaagca
    13201 ttgatttgaa tgcccatttt tcgcaaagcc aacttggcaa ttgcgcctgc tgctactctg
    13261 gctgccgttt cacgtgcaga gcttctgcca cctccccgat aatcgcgtat gccatatttt
    13321 tgctgataag taaaatcggc atgtgatggt cgaaaaacat ctttcaaatg attgtaatct
    13381 gcacttcttt gattttcatt ccaaatcaca aaagcgatag gcgctcctgt agattttcct
    13441 tcaaaaattc ctgaaagaaa ttcaacctta tcctcttctt gtctgggcgt tgagatattg
    13501 gattgtcccg gacgacgacg attcaattcg ttctgaatga atgcttcatc tatctctatt
    13561 cccggcggac atccatcaat aacgcctcca atggctttgc cgtgcgattc gccaaaagag
    13621 gtaaatgtaa aaaacttgcc aatggtgttc gacataacta aaaaatcgtt attactgaaa
    13681 aagaaaatcc aatggaaaag gctgtataac cttttccatt gcttaaaatt caaaaataaa
    13741 attacttatt gcaagagcaa ttgggatctt cacaactgtc gctttcacag tcgcaacctt
    13801 cctcagaaga acaattaccg ccacaactgc acgaattgtt cattaaagcg gccaattcgg
    13861 cttctgaagc atcacgtact tccaacacct gacccttgaa atgcagattc tgacctgcca
    13921 acggatgatt caaatctacc gtaacaagct catcggttac cgtaacaatt tgggcattaa
    13981 atcgattgcc atccgtatcc attaaaggaa caacatttcc ttcaaatact acgttttcat
    14041 caattttccc atccacttca aagatttttt tgtccaaatc gataactgct tcttcatcgt
    14101 aagggccata tgcatcttca tcatttatgg tgaactcaaa agaatcgccg gcttgcaatc
    14161 catataaatt ctcttcaaat ttgggaagca tcattcccac tccataaata aaactcagcg
    14221 gacgttcttc ggttgcacgt tccattaatt ccaattcacc ttgattttct ccgtcaacaa
    14281 ataaatcgta tttagctgac actgatttgt tttcttcaat tctcattttt tactctgtta
    14341 aatttatatt caattaattg tttgtttcta atttttttgt cctttattac caataaactg
    14401 atttttagtc aaaaagttca tttaatattg aaatttttta ttactaatac caggtaatgg
    14461 gcgtagaacc atacccgctg cgcttttcat acttcaaatc gcgcaacatg ctggaattga
    14521 caccaattct gaaataatag gactgaaaag gtccaaaagg aacaaaactg gctgacatgt
    14581 tccaacaatg caaatcgcgt gaaacattaa tactggtgta ggtaaattgt tttgcaacaa
    14641 aatcatagga agtactcgag ctgattctcc atttgggcgt cagcgaaatg tctcccgaaa
    14701 agcttaagtt gtgggtaaat cccattttgt attccatttt ttctttgtcg aaaacatttg
    14761 tattggcata tcgaacagaa taactgatat tgatattcca cggaatggtt actttctggt
    14821 aaccattagc atcagtctga atttcctgat ttgatttttc cgaaagtccc aactgattta
    14881 tttccggtga agtcgtaatg ctttcatcat caggattatc taaattttcc ttcgatgaag
    14941 tattgttctt gacatctgtt tttttgttgt ctccgcgcga aaaaagtttt ttgaaagtat
    15001 cgttgttcaa agccagactg taagaggcat ttgttccgag aaatttggga aatttatgat
    15061 aattccaacg caatttattg attctaaccg gattcccata ctgatttaat ccatacatat
    15121 aagggtcaaa tgctccacta agatttaacg aatatttgtc gctgaattta attcgaagat
    15181 tggtactaaa atttgaccat tgcatggaat cggctgccag attatatcct ccacttaccg
    15241 aaaaattatc aatcagactg acttttttaa aaggtacttt ccctgttgta tcattgtcgt
    15301 ttctcagttt catctcaata ttgttggcaa atgaaaaatt gatatttccc gacttacctg
    15361 aacccggata tccgtacaaa cttccctgat agtgggaata aacaacttct tcttcaatca
    15421 tgctggcagg attggaagga tctttggtcg tttttttgta ggaatcgtaa tatccccaac
    15481 gcggatctcc aaaatcggga gcatatccga aaccgatcgt tggagtaaaa acatgccgaa
    15541 tacggtcaat tttatctcca aaaatggaac gaataggaat aaaaaagcca taaagtttgg
    15601 tagaagccga aacgcccata ttaaaatcgt aaactctgtt aaaaccgcta accgtatcgg
    15661 ttatttcttt ttgtttatcg ctatcccaat tcttatccac agatcgtaaa taccagcgtt
    15721 cagtgtaatt gaaagaagga gagatgttta tatacttgaa taaattaaaa gttgcgctca
    15781 ccggaatact gtgccgcatt ccgtttcgcc agtctttgga aagcgaagat gacaacagca
    15841 aagattcttt tgtatcaatg ctattggcaa aagtacccga ataggacata taaatttttt
    15901 cgtaccatct ttcattacca acagcatttt ttcgcttgaa tggagaaaaa cggctgacag
    15961 caatattaag agtcggtaat gtaagactga tggtggaatc ttttgtacgt tgatttatca
    16021 acatacttcc cgacaaactt agattggatt gagaaggaaa tctttttgta aaagatatac
    16081 tggaaccttt ggtgttttcc gaattcaaat ttggattaaa atagctgttg atattacttc
    16141 ggttgtaacc actggttgaa aaattaacac tggatgtaaa ggtaaaatac ggatttgcct
    16201 tctgatcctg cgaatgtgtc caacgaatat tcatattggt cgccttactg taatcgggta
    16261 aatctttttc gccggtaaca tccacccgat agctaaaact gaaattccca ttatatttat
    16321 acttttttat gtatcgtgaa ttggcagtaa gagcccaagt tcctttcgta taaatatctc
    16381 ccaacaattc caagtcggca taatcgctca aggcaaaata atagcctcca ttagtcaacc
    16441 cgaaaccgcg attcagttca tcagcatagt tgggcattaa tatacccgaa gaatatttgt
    16501 cggtaaaagg gaaaaaacca aaaggaacag caattggtaa aggaacatca gccaacacca
    16561 ggtaagcagg accggtaaca atgtagtctc cgggttttat ttttcctttg gtcaaatata
    16621 gataaaaatc gggattatca tgcttatcgc aggtagtata tttgccgtcg gcaatcagca
    16681 aaatattatc atccagtttt tttgttttat cgctgataac ataaccttct ccctgctggg
    16741 taactgcatt tcgaatatat ccttttttgc tttgtaaatt gtaggtcagc tctttcgaat
    16801 aataagttgc ttcaccctca ctgaaaacag gttctccaat tttatttccc agcgaatcag
    16861 tcgttcctct ggcataaatc aggctgctat ccattttaac acgaataaaa tcggcctgca
    16921 atgttatgtt tttatacttc acatcgcctt tcccatgcaa aaaacctgtt ccatttccgt
    16981 aaaaaacaat agaatcggaa gcagaataaa tgatttcggc atcaattttg ttagggtttt
    17041 ctctgggtac agatacggtt aagctttcgt ttgtagttaa aaccgaatct ggcaatgctt
    17101 gaagagtatc ggaaggtgat ggatttaaat tctttgcttg cagacaaaat gaaatattca
    17161 accctgtaat taagaggaat aatttacatt tcaggctatt gtgaaattta aaattatggt
    17221 gcatccttgg gattaaacat tttcaattaa gctgcaaatt taaggaaaaa aaaagctatc
    17281 tacagtatct atcccctttt ttatgattaa tttcaaattt atccaatgga atttgaaata
    17341 aaaaagatta attttgcaga aaataaaagg attgtaaaaa cttggaaaat taatgaacgg
    17401 gttatagttc atcagaagat aaaaaaacga ttcgatgcgg aaaaagacaa aaaatttttt
    17461 ggcatgaaaa ttccatttca aaagtactta ttgttgcctt acggtttcct gatattgttt
    17521 ttatccattt tcgcttccag tgttgctgcg caggaaaaaa aatttgtagt ggtaatcgat
    17581 gccggacatg gaggacatga tccgggagca atgggtaaaa tttcaagaga aaaagatatc
    17641 aatctcgctg ttgccaaaga attggctaaa ctcatggaaa acaataagga attgaaagtt
    17701 gtactcacac gaaaaaccga taaatatctt accctgcagg aacgcgccga catagccaac
    17761 gaaaatcatg ctgatctttt tatatgcatt cataccaatt cttcgaaaag ttcttcggct
    17821 tacggggcag aaacttatac actgggtttg gccaaaacaa atgcaaatct tgaagttgcc
    17881 atgcgtgaaa actcagtgat tttgctggaa gaaaactatc aaaccaaata ccagggtttc
    17941 gacccgactt cggttgattc ctatataatg tttgaattta tgcaggacaa atatctggat
    18001 aaaagtatcg aatttgcttc gttggtgcaa caacacctcg atagaaacgg acgtttggac
    18061 agaggcgttc gtcaggccgg attttgggta cttcatcgct ctgcctgtcc cagcacacta
    18121 atagaactcg gatttatttc caatccggaa gaagaacttt ttttaagttc cgaatatgga
    18181 caaaaaaaga tggctcaagc tatctacacc gcatttttag atttcaaaaa agaacacgac
    18241 aagcgaaacg gcaagctaaa ttctacgtct gtttccaatc aagctccttc caaaaaatcg
    18301 gaaaacatag tttcagaaag tcaaaacaat tattcaactc ctatacagca agcagaaatt
    18361 gaaaacatca atactgccaa cattacgaat caagaaaaac aaacagaaaa tattttaccg
    18421 tcaacacaac aacctgtttt caaattacaa attctggctt caaaaaagaa attatctccc
    18481 caagcgaatg aacttaaagg cctaaaatcc gttgattatt acattgagaa cgaactgtac
    18541 aaatacaccg ttggagctga aaccgattac aataaaatcg ttcaattaaa aaacaatttg
    18601 tcggcgaaat ttccccaagc ttttatcgtt gctttcgtta acggtaaaaa agttgatgtc
    18661 aaggaggctt tgcgcttgac aggcaaataa caaaatttct aaaactgtgg tttctgaaaa
    18721 actctaagaa aagctctaaa tatctttttg atagcaaaaa caataaattg tttatgaaaa
    18781 aaaataaatt ctgataatat tcaagttatg aaaaaaaatt tttttaataa agaagtaaaa
    18841 atcggattga tggtttttgt agccattttt ttgttgtatt tcggtttaaa ctttctgaaa
    18901 ggaattgata tttttacctc aacccgttct tattatacta cttatgaaaa tttggatggt
    18961 ttggttgcat cttctcccgt atttataaaa ggatataaag ttggacaggt tgacgaaatt
    19021 caatacgact tttcaaagga aaaaccattt ttagtgaaaa ttactatcga aaaaaaaatc
    19081 aatcttccca aaaacaccaa gattcaatta ttcgacaacg gtttgatggg aggaaaagcc
    19141 attcagttga tttttgcgcc ctacgcttct tctcaaaatt tttgcaacaa cggagataca
    19201 ctccaatcgg aaatcaaacc cggactcatg gccgacttga gttccaattt gttaccaaaa
    19261 attgaaagta tttctacaca aaccgattcg cttttgcgtt ccattcgttc tattaccgaa
    19321 ggcgaaaaat taaacaacag cctcacttcc attgaaaaaa caactgccga cctggcagaa
    19381 acttcggcac aaatgaaaaa tctgatgaaa tatcagcttc catctatcat gagtaatttg
    19441 aatggagctt cttccgattt caaatccgta acttccaatc tgaaagaaat taattttgct
    19501 tcaactatga acaacgttga tcaaactgtc tccaatttga atttgcttac caataaaatc
    19561 aataacggag aaggaagttt gggcatgctt ctcaccgacc agcaatttta tctgaatttg
    19621 gcaaatactt cctccagtgc caatcaatta ttgatcgact tgaaaaataa tccgaaacgt
    19681 tacgtacatt tttcactttt tgggagcaaa acgaaataga atttatttag aatttctcta
    19741 aataaaaatt tgaaaataat tttgaattta tttttttaac atcattgaaa cacttgttaa
    19801 taaaaggttt caatgtaaga aatcggaaaa aattcaaatt ccgatttctt tttgttaaca
    19861 cctttagaat caggataatt tttaaaaatt tattatgcaa ttaactgaat atgaatttgt
    19921 ttcatcaaag cactgacagt taaccaaatg aaaaatctga ttttttttgt agaaaaaaag
    19981 atttgttctt gtcaaaaaaa ttttagattt ttgcctaact aaatgcagcc gtacgtctaa
    20041 gtagaatttt caaaagtaaa attttgaaaa tctttttttg gctgaaaatc tcacttgttt
    20101 acttttttga atggattaaa aaaatgaaga tgcacgaaca actatgggaa cgctgcctta
    20161 aagttatcag ggacaatatc aacgaagcat cgtacaaaac atggtttgaa cctattatcc
    20221 ccttgcagta tgaaaacaaa cgttttgttg tacaggttcc aagtcaattt tttgttgagt
    20281 atatcgagga aaaatacatc gatttgctta gtagaactat ttacagagaa attggatccg
    20341 gcacccaatt ggaatatcgt gtattgatag atcaaaccaa tggaaaaggt acacaaatcc
    20401 cgagcacagc tgaacagcca aaactgaaat cctcgaacac cacgcaagga acaaaaaatc
    20461 cttacgacaa gtcttcattg cccaatatcg atccacaatt gaacatgaaa tataatttca
    20521 atactctgat agaaggaaaa agcaataaac tggctcgcac agcgggtgtt accattggag
    20581 ccgaaccggg gaaaacagct tttaatcctc tttttgttta cggggagtca ggtgtgggaa
    20641 aaacgcattt agccaatgcc attggggtga aagccaaaca aatgcatcca gataaaagag
    20701 tgctttatct ttcggccaat actttccaaa ttcagtacac cgatgcggta cgaaacaata
    20761 cggtcaacga ttttgtaaat ttttaccaaa ccatcgaagt tttgattatc gacgatattc
    20821 aggaattttc gggaaaaacg gccacccaaa acactttttt ccatatattc aatcatctgc
    20881 atcaatcggg caaacaactg atattgactt ccgaccgttc tccgttactg atggcaggat
    20941 tggaacaacg tttgctgacc cgattcaaat ggggactttc ggccgaaatc gaaaaacccg
    21001 actttgaact tcggaaagaa atactaaaaa acaaaattta ttgcgatgga ttggatatac
    21061 cagaagatgt agtgaatttt attgcccaaa atgttaccga taatattcgt gatttggaag
    21121 gtgttttgat ttcactgctg gcacattcaa ccctcacaga tatgcccatc gacctgcaac
    21181 ttgccgagaa agtcgtcagt cgtattgtaa atatccccaa aaaaataaac acggtagaaa
    21241 aaattcgtga tacggtttgc gattatttct ccatcccggt agatgccatt tttaccaaaa
    21301 gtcgtaaacg agaagtggtt caggcgcgtc aaatagccat gtatctgtcc aaacagttca
    21361 ctaaaaattc tctctcttcg attggaaaag ccattggaga gcgcgatcac gcaactgtac
    21421 tccacgcctg caaaattatt agtgatctta tggatacgga caaaaaattc agatcgaacg
    21481 ttaaggctat tgaggaaata cttcgaaatt aatttatggc tattcgatca ataaatatct
    21541 tttaaaagct gtttaattat tgtttaaacg gctttttttc tgaaaaaatc gggagaaaat
    21601 gcttattagc tccaaaaaaa ttactatttt taccgtcgaa acttaaacaa caaaagctat
    21661 ggtttttaga tttactatat tatcggacga agtggacaat ttcttaagag taatagatat
    21721 agatgctgaa gcaactttct ttgacttaca taatgccatt ctggatgccg taaacttcaa
    21781 aaaagaccag attacttctt ttttcctctg ttctgacgac tgggaaaaag agcaggaaat
    21841 tacccttatt gaaatggatt cttcctcaga atatgataat ttggtgatgg ataaagtaaa
    21901 attggaagag ttgcttaccg acgagaaaca aaaactcatg tatgtgtttg atatgattgc
    21961 tgatagagca ttttttatag aactaagtga aattattcca cgaaaaaaac ttgaaaagcc
    22021 ggtttgcatc aaatcaatcg gcaatccacc cgaacaaacc ttaagcgaaa atgatgtgaa
    22081 tcaggctcca aaactgaacg tggaagaaaa cttttatggc gacgaagatt ttgaactgga
    22141 tgagttggat gaagaaggat ttagcgattt caatttcgat gacgacagca atctgttcaa
    22201 tgatgacacc aacttttgat ttcaactgat ttttttcact gacagaaaat aacgggttta
    22261 ttttctgttt ttttattttt tacgaatgca atcaacctta atagtaattc ttggccctac
    22321 cggtgtaggt aaaaccgaaa taagcctgca agtagcccaa aatttacaaa cgcccatcat
    22381 ttcggccgat tcgcgtcaaa tatacaaaga actgaaaatt ggtacagcag cacctacatc
    22441 cgaacaactt tccacagtaa aacattattt tatcggttct cattcaattt ttgaaaattt
    22501 cagtgccgga caatttgaac aggaagctct cgcattgctt gaaaaacatt ttgcgaccaa
    22561 tcacatcgcc ctaatggttg gaggttcaat gctttacatt gatgctgtat gcaaaggact
    22621 cgacaacatc ccgacagtag ataccactac aagaaacttc tggaaaaaac aatatgaaaa
    22681 tcagggactt gaatacattc gacaagaatt gaaaaaattg gatcctgtat attatcaaca
    22741 agtggaccca aataattaca aaagaatttt gcatgcgctg gaaatctgtt ccatcaccgg
    22801 aaatcctttt tcggcattaa gaaccggcgt tcacaaaaaa agagctttcg atattgttaa
    22861 aatagggctg aacagacctc gtcatgaatt gtatgaacgc atcaatgcaa gagttgatca
    22921 aatgatggaa gaaggtttat tggaagaagc aaaacaattt tacgctttta agaacttaaa
    22981 tcctttgaac actgttggat ataaggaact tttcgaattt atggacggta aaattacact
    23041 aaaacaagct gtcgataaaa ttaaacaaaa cacccgacat tatgccaaaa gacaattaac
    23101 atggttcaat cacgacaaag acattacttg gtttcatccc gatgaaagag aaaaaataat
    23161 ccgtttccta caaaaccgac tttcttaact tctcaagaat gaatattaaa tacaactatt
    23221 ccctcctttc gcacaatact tttggcattg acgtaaaagc cgaagaattt atcgaatttt
    23281 atacggtcga cgaagcttta gatttgtcaa aaacgttgaa tcataaaaaa tggtttgtgc
    23341 ttggacaggg aagcaatctg ctgtttttaa acgattttaa gggtgtcgtt ttacactccg
    23401 gaattcggca aatggaaaaa ataaaagaag acaaccaatt tgtgtatttt gaagtgggct
    23461 cgggagttgt gtgggatcag tttgtggaat tttgtgtaaa aaataactgg agtggcattg
    23521 aaaatctttc attaattccg ggagaagtag gtgcagcagc cgtccagaat attggcgctt
    23581 acggtatgga agttcaggaa gttattgaaa aagtgaaagc aattgatact gcaacttctc
    23641 aattgatgga attttccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
    23761 tgaaaaagtg aaagcaattg atactgcaac ttctcaattg atggaatttt ccaaagaaaa
    23821 ttgtcaatac ggttatcgtc aaagtatttt taaaaatcaa tacaaaggtc gatacatttt
    23881 gacttctgtg gtcttccgtc tgtcgaagaa acctaatttc aggcttcatt accagcattt
    23941 ggaagaagaa gtcagaaaaa gaggaaacat aaacctgcaa accattaggg aaaccattat
    24001 acaaataagg agccaaaaat tacccgattt cagaacaatg ggcaacgccg gcagtttttt
    24061 tgtcaatccg gtcgtttcca aagtgcattt tcagcgtttg caaagtgaat atcccgaaat
    24121 acctcattat tatgtatctg aaacagaaga aaaaattcct gccgcatggc ttattgaaca
    24181 atgcggctgg aaaggtaaac aggttggaaa agtcgctgtt tatgcaaagc aacctttggt
    24241 aattgttaat tgtggtggag cttcgggcag tgaaattaaa caattggccg aagaaattca
    24301 actttcagta aaaaacaaat ttgatattca acttcaaccc gaagtcaatt acatcgaata
    24361 atttctttat ataaatatct tattttcaac tttcaagaag atttcagtct actttttcac
    24421 ctatatgcct aaaaaagaaa gtgtcctttt gggacaccct cttaaaattt agatttgatc
    24481 tacttgtttc caatcaaaat aatttttcag caaacaaggt tcaattcaga ttcagtaacc
    24541 attaaattta gaagctactt tatttggtct tatcggaggg tttaccgata ttttcacgca
    24601 tagcagtatc agcttcaata tttttcaatc tgtagtagtc catcacaccc agattcccgt
    24661 ttctcaatgc ttcagccata gctttaggta cttcagcttc agcttcaatt acttttgcgc
    24721 gcatttcctg tgattttgcc cgcatttcct gctcaagggc aacagccata gcccgacgtt
    24781 cttcagcttt agcctgtgca atatttttat cggcttcggc ctgatccatt tgcaactggg
    24841 caccaatatt ttttcctatg tcgatatcgg caatgtctat ggataaaatt tcaaatgccg
    24901 tgccggcatc caaacctttt ttcaatacca acttggaaat ggaatccgga ttttccaaaa
    24961 ctgccttatg cgattcggcc gaaccaatgg aggaaacaat tccctctcct actcttgcca
    25021 gtattgtttc ttctccggct ccaccaacca actgtttgat attggcccgc accgtaacgc
    25081 gcgctttagc aatcagctga ataccatctt tagcaaccgc agctaccggc ggtgtatcga
    25141 taacttttgg gttgaccgac atttgaacgg cctgaaaaac gtctcttcct gccaaatcga
    25201 tagcagttgc cattttgaaa gttaagtcaa tattggcttt agaagctgag accaaagcat
    25261 gaaccactct ttcgatgtgt ccgccggcaa ggtagtgagc ttccagttca tcgcgcttta
    25321 tttcgtgcaa atcggctttg tgagcttcta tcaagcattg aacaatagag tgaggcggaa
    25381 cttttctgat gcgcatgagg aaaagctgca ccagagaaat actcacaccg gaaactttag
    25441 ccgagagcca caacaaaaaa gggacatagt aaaaaaacaa taataaaaca acgaccagaa
    25501 caatcagtaa tacaatttca attcccatca taatttataa atttaagcat gttcattatt
    25561 tgaattttcc gaaatacgtt caaccaacac attggttttg aaaacttcct ttaccaaaat
    25621 tggggttcct tcatcaataa attcatctac acttttggct tctatggttt gtccatttac
    25681 tttgactttt cccattggag caagtcttga gagcgcaatt cccgtgtctc ccgcatgaat
    25741 gttcaattct tcgacagtac caatactgga atctattttt gcctttaaac tgattctttc
    25801 caatgtttta cgtcgcaaaa aaatccaaac tgcaacggcc aaaagaacaa cactgcctgc
    25861 aatagaaaca ttccctgctg ttgttcccaa ctgggaatag gaatagtaaa ttccggcacc
    25921 aacaaacaaa aaaccggcta ttcccgcaaa cgaaattccc ggcaccagaa aaaattctac
    25981 cagaaagaaa aaaacaccaa ccaaaatcag aataatagct attgctatgt ccattgtctt
    26041 aaaatttttg gtaaaaataa aaaataaatt tcgaaataca actacttcat taagtttata
    26101 ttttctttat ttctgagttc aaaggttttt gtagttaact gatcttccat ttccgaaatt
    26161 tccttttcca aagtcagaat ttttggcgcc aattcatttt gagtttcttc ctgctgactg
    26221 tattgcattc tcagttgttc caattgaata cgtttttgat ttagttgcga atttaatttc
    26281 tccagttcat tccacaagtt acgggtctct tcggttttaa actgattgtc atccgtgtag
    26341 attacagaat cgttcactac aaagaaaatt ttatgaaccg aatcttcatc agcgaatgat
    26401 gtttgaggaa taatatccga attgaaattt ttggcttttt tgaatagttg taactgtgct
    26461 gcttttctta tatattctga atcctctgac tggagaatgg tttttttagt gttggggata
    26521 aaggaaaaaa taattacttt tccttccggc tgaaagcgat cggtggcaaa ccatcccact
    26581 tgatgaatgt cgtcaataac catcatatag tcattgtaaa tcgaattata aggcattcca
    26641 atgttttcgg gttgaaaata ggtgttggtt tccggacgga agcgggaaac aaacaaatcg
    26701 tagcctccaa tagaattatc tccgtccgac gcaaaatata tggtaacacc gtccaaaagt
    26761 aaaaaaggat aattttcgtt ggctttggta ttgattatat cggaaaccga ttcgggtttt
    26821 gaccattcat ccaaaagctt gtaagaggta aaaatatcca tttgaccgtg aacagtatcg
    26881 gaaaacaact ttctatcctg acgttgagtg gtataagtta ttttaaaatc cttttttttg
    26941 cctattttca caggtgttgc cgatagcgaa cccaagtcat tagtgaattt gtaaaattcc
    27001 aaaaaatggt ctttattaac caccacacta tcaatgattt gaatatcgtc cactctttga
    27061 atcatttttt ctgccagttc agctttcttt tttgcttttt gaattgttaa gaattccttg
    27121 tcggtttcat ttatggtttt tagatattct tcgtaggctg ctaccgattg ggaaaaacgg
    27181 taagttttca tatacaattc tcccaaatac ttgtttgtca aaggaaatcg attacctgaa
    27241 agttcaaaat gttttatggc attttcatca tctttcaatt cgtaacagca acgcgcatac
    27301 cgataattat acaaagcatc ctttggcctt ttctccaata atattttata ggcatcgcgt
    27361 gctttctgaa actggttttg ctgaaaaagt cggtctgctt cggcagcagt ttgacccaaa
    27421 attgctacac agaaaaacaa caatactgta atcaaagaaa tttttgaacg catgacaaat
    27481 attttcaaaa tatataattt gttgaaatta aggtacttaa ttaacaatct tgcataaatg
    27541 ctttagaatt agaaattcgt atttttcgat gcattttcaa taattttatt ggcttgaatt
    27601 tcggcatctg cattcagttt atcggcttgc ttgcgtgctt cttctaccag cttacgagcg
    27661 gattgttcgg caactttttt ggcaattgga ttggctgctt tctcaaccaa ttcgttgcct
    27721 ttcagggtgg cctcttcaat cagtttatcg ccagcttttt tagcttccaa tcttatttta
    27781 tctgcttgaa cctgtgcttc ttttaaagca gcctcacgtt ctttccgagc cttttcgaca
    27841 acttcttttt tggctgcatc tacttgtgta gcaacttctt ctttaatttt agcttttgtt
    27901 tcctcaagcg cttcgttaaa tgtatccaac aaatccaatt tcacttttgg tttatcgtag
    27961 gttccaccaa ttgttacgtt aaagtttgaa aagcgaccgg cattgagttg atccggtaat
    28021 tgaacaattc ctttaaaatc aagcgattga tccaaaccca ttgaacctcc caaattcatt
    28081 ttaatatcgc ccaattttat gtcgaatggt ttggtaaaca atttcccgtt ggatatttcg
    28141 aaattcacag caaggtcttt caatgatgga ttgcttaatt ctggtttttt caggcctgat
    28201 gccaatgccg acaatgccgg aacatctttt aaagcaactg acgaagttga aaattttccg
    28261 ttggctttta atgaactcag attcaagctc atgtcgttat tcaataaagt attcaacgac
    28321 aatgtggtag aaaaatttcc ggtagctttt tcgaaaaatg gaacaaattt ttgaaatgaa
    28381 ccaacttgcg aaaaaatttg attaaaagcc acatttttaa tgttcagatt tatatccgcg
    28441 tatggttttg taacgtttgc cgtgctgtaa agtccatttg ccaataacgt tcctccaaag
    28501 gcctccaaat tgagattttg aaatttaatt tcgccatttt gtaccaaaag ttttccctga
    28561 atattggaaa aattcatttt gtcgtaaagc aattgtttga aaaatgcctg gagtgtgaaa
    28621 tcaatatttt gaggaattac aaacaattcc attttttcat ttttagtttc tgttgtttcg
    28681 gtgaggaaat cattcacgtt caaataattg gattgcaagt tcagctgtcc ttttaaagtt
    28741 ttgttcttca aagcataggc caggaaattt tcaagttttc cgtttgcctg caaatcgttc
    28801 ttgccgattt tcaattgaaa gtccgataaa tttacatatc ggttattgaa aaaaacctga
    28861 gctcgattaa cagaaatatc ttgtggcaaa gagcttgttt tcagaaaaat attgttggcg
    28921 agcaattttc cctgaaaccc aaatttatcg tacaaattat tttccagata cgacattttc
    28981 ccattcagat tcaaatcgag gtcgagcaag ccgttcaaat tcgtgccggg ttccaaagga
    29041 tagaaggatt taaccattcc caaatcaatt cgccctgaag ctttggcgcg aatatcggga
    29101 tcggaaatga tggaagcaat tttagctgca gcattgaaag tattactccc caacgttaaa
    29161 gaaaacttgg aaaggtcaat taaagtactg tcgagcgaac caccgggatt gacaatttgg
    29221 aaggcaagct gaatattttt aacggattga ggcaatgaag gatattgaaa ccagccattg
    29281 tttatcgaca aattgacatc aaaagccgga taattttcgt cttttatctg acctttgaca
    29341 aaaccattca aactgacttc tccaccggtt ttcaaatctt tgaaagaatt ggaatagatg
    29401 gccggaatca tggaaagaat tgattttaaa tctactttat tggcaattaa cttaatgtcc
    29461 atatcgaaac cttcgtccaa catttttacc cacccttcaa aagaaaatgg aatagcgttg
    29521 attcgtgaag aatttttgct aaaagcaaat ttcatgtcgt taatattggc gtcgatatct
    29581 acattcagtt ctgcattggc ccttaaaagg taaggaatac cattcatcca aaagctaatg
    29641 gaatcgatgg ttgtctgtgt agacagaagc gaactgtcgg cagttaaatc gccactggtg
    29701 tgatgattca gattactgat aatggctttc atgttgctct gttcgtccaa ataagcgata
    29761 ttggcatgtt gaataaaaac atcctttaat tttaaatgaa atttcatggc agcagtatcg
    29821 gttttttctt ctgccgtatc tttcataatg ttccaattag ctcttccatc agcaagtaca
    29881 tgagccatta tatgcggatt aactacattg atcttttgaa tatcgtaacc ttttccggaa
    29941 agcaaacttt tcaaatttac aactgcctca attttatcgg cagaaaccag cgtgtcagca
    30001 gcaaaatcac cttctccgat aacttttaat ccgttaatcc tcaccgaagc atttggaaaa
    30061 tgtcgaataa aactcaattg aacattttga aaatcaactt ttgccgtcaa cattttattg
    30121 atttgcaatt tcaccgcatt gaaaattttc ccctgaaaag caaatggtaa aatcatcagg
    30181 acagcaaaca aaacaatgag gattgaaaaa attattccaa gaattcgtcc atttttcctt
    30241 ttcatattgc aaaattttta aactgaatca aaacaaaggt acaataaact gccgaaaaaa
    30301 gtaaaacttt ttttggtttt tcaattattt ttctcttatt ccggaaaacc tctaactttg
    30361 cactgaaaat gaaaagttct caatcatgag tcccctccaa aaagttactc aaagagaatt
    30421 agatttttaa agacattaag ataaaaaagg atacaaaata ttcaaaaagt tttttgaaaa
    30481 atttgcatat attcttatat ttcagtatct tagaagggtt ttggagggga tgatgatttt
    30541 aaaagaaggt cagggctgga gcgacgagca gttattttgg ccatcataaa aaatgtctta
    30601 tcaagacaga aaatggcaac tatcgttatt ttaa
//
LOCUS       DAOTAX010000020        30179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1257_length_30179_cov_30.877374, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000020 DAOTAX010000000
VERSION     DAOTAX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30179
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_30179_cov_30.877374"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PLM04_03710"
     CDS             complement(<1..1334)
                     /locus_tag="PLM04_03710"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HON02217.1"
                     /translation="MLRNQLKILALLGTIFFLNSCLGDGNVKEISSDPSFVSLTFSAN
                     NKIPNLKDAVFSLQYDSVIVNLDSLPYQTRIDSVYATFTFKSTSAAYLVYENDSTALI
                     SGNEAFDFTKIKAIINIASDGVTRRKYLIKVNVHQVEPELYIWQKVNDAAHSLNVQHQ
                     KAVYFNDRLYLFVNDRQQNYLFVSPNGVSWTQDVISGLPTNATFEDITVFNNQLYVTC
                     GNSNIFSSGDGLTWTRQTYSEYEFTSLIFVLNDRLWAVSHSLTDGKLRFASSVDGTEW
                     LIYNEIPENFPVRGFASVVFNTKVNKQKGLVLTGFSSDGTALKTNWNTEDGIYWVDFS
                     AENASLDSIGRGASVINYDDKLLLFGQDNKPIPGSYFRQSVDQGFSWQVPDSNLNNLP
                     KEMGIRHYSSVVVYRPRTYSSTASKQEIESSNWIFIIGGKNAYDEYLTDVWKG"
     gene            complement(1452..2216)
                     /locus_tag="PLM04_03715"
     CDS             complement(1452..2216)
                     /locus_tag="PLM04_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HON02218.1"
                     /translation="MKIISYNVNGLRAALTKDFIQWLQAENPDVLCLQETKAQPDQID
                     TLLFAELGYTAYLHSAQKKGYSGVATLTKIPPDYVYFGMDNPEFDTEGRVLRTDFGDI
                     TVINVYVPSGSSGGERQDFKMKFLEEFLKFIQNLLRERPNLVVCGDYNICHKPIDINH
                     PERQIGVSGFLPEEREWMDRFQALGMVDSFRVFDQSPEKYSWWSYRGGARYRNAGWRI
                     DYHWVSEPLRSRLKAAAILSEAFHSDHCPVMVELDV"
     gene            complement(2257..3024)
                     /locus_tag="PLM04_03720"
     CDS             complement(2257..3024)
                     /locus_tag="PLM04_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON02219.1"
                     /translation="MIEVKNIIKTFDGVPALQDISAVFETGKVNMIIGQSGSGKTVLM
                     KCIIGLHQIDSGCIEYDGRNLPKMKPQEILMLRREVGMLFQGAALFDSMTVLENVMYP
                     LEMFAHESNEEIRERAYFCLRRVNLEERSYDLYPSEISGGMQKRVGIARAIALRPKYL
                     FCDEPNSGLDPLTSMIIDDLIYEITHEFNITTIINSHDMNSVMEIGEKIIYIEKGVKA
                     WEGTKDEIFDAENESFNNFVFASKLFKQLKMLKQNPY"
     gene            complement(3155..3895)
                     /locus_tag="PLM04_03725"
     CDS             complement(3155..3895)
                     /locus_tag="PLM04_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON02220.1"
                     /translation="MNLFQETGKYFLLMKRVFVQPDRSKMFFRQFPKELDKLGLQSLP
                     IVLIISVFIGAIMTIQTKLNTENPLLPTYSTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDALEIMGVNSANYLILPKIVAFVVMMPFLVIFSMAVGLIGGYL
                     VGVFSDVITVADYLLGIQYAFNPFYVFYAMVKSLVFAFIISSVSSYYGYYAYGGALEV
                     GKASTNAVVNSSILILFFNLLITNVMLG"
     gene            4599..6119
                     /gene="purH"
                     /locus_tag="PLM04_03730"
     CDS             4599..6119
                     /gene="purH"
                     /locus_tag="PLM04_03730"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HON02221.1"
                     /translation="MEKKVIKKALISVYHKDNLEEIITKLHAAGVEFISTGGTKSFIE
                     SLDIPCEAVEDLTGYPSILGGRVKTLHPKIFGGILARRDNAQDVQQMSEYEISGIDLV
                     IVDLYPFEATVAAGADEPTTIEKIDIGGISLIRAAAKNFKDVVVIASKEQYEPFLEIL
                     NSSGAQTSIEERRWFAKEAFAVSSQYDTAIFNYFDEPYQSAFRWYENHPKVLRYGENP
                     HQRGIFFGKFSEMFEQLQGKEISYNNLLDIDAAVNLINEFDDITFAVLKHNNACGIAS
                     RPSLLEAWKAALAGDPVSAFGGILITNAIIDKETALEINKIFFEVIIAPDYDLEAIEI
                     LSQKKNRIILIMKNNTLRPKQFRSLLNGVLMQDRDLHTETAADLKVVTEKSPSEAEIE
                     DLLFANKIVKHSKSNAIVLAKNKQLCASGVGQTSRVDALVHAIDKARSFDFDLKGAVM
                     ASDAFFPFPDCVQIAHEAGITAVIQPGGSIKDQDSIDFCNQKGLSMVFTGYRHFKH"
     gene            6166..7185
                     /locus_tag="PLM04_03735"
     CDS             6166..7185
                     /locus_tag="PLM04_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HON02222.1"
                     /translation="MGFFSFTQEIAIDLGTANSIIVYNDQIVVDEPSVVALDKRTDRL
                     IAVGERARQMQGKENESIRTIRPLRDGVIADFYAAEMMIRGLIKMIPSKNRFFTPSLK
                     MVIGIPSGSTEVEMRAVRDSSEHAGGREIYMIYEPMAAALGIGIDVEAPDGCMVVDIG
                     GGTTEIAVISLGGIVLNKSIRIAGDDLTDDIVEYMGRMHNIKIGERTAELIKINVGAA
                     LTDLEDAPEDYIVRGPNRMTALPIEVPVSYQEIAHCLEKSISKIEAAILSALELTPPE
                     LYSDIVQHGIFLSGGGALLRGLDKRLTDKINIPFHIADDPLHAVARGTGIALKNVDKF
                     SFLLR"
     gene            7297..8142
                     /gene="mreC"
                     /locus_tag="PLM04_03740"
     CDS             7297..8142
                     /gene="mreC"
                     /locus_tag="PLM04_03740"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HON02223.1"
                     /translation="MKNLINFLIHHSGFFVFLLLEILSLMLVFNNRDYQKSVFFSSSN
                     TIISSFYNWNNYIVGFFNLSKSNQQLAEENTQLKNQLLDLQNKLEALQPSGTDSLSYK
                     IPPEKQYRFIEAKVINNSTNKLQNYITLNKGALDGVKPDMGVINDEGIVGIVKNVSQH
                     FSVVIPVLNPKIQINCKFKRNNYIGSLVWDGKDYRFALLTDIARHVPIKMGDTLVTSG
                     LTTTFPEGIPVGTIENFNLKESDAYYEIKVKLATNFRTLSYVKVIDFKNAGELKELEK
                     AAYEE"
     gene            8144..8644
                     /gene="mreD"
                     /locus_tag="PLM04_03745"
     CDS             8144..8644
                     /gene="mreD"
                     /locus_tag="PLM04_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HON02224.1"
                     /translation="MAITVFQNIIRFVLLMMVQIFVLNNIQLSGYINPYIYILFILSL
                     PYRTPQWLTLILAFVTGLMVDIFMNTMGTHAFATVLMAFTRNGIINLITDFEKDENPT
                     PSFYTFGIGVYIRYIVLLVFIHHTALFFLEAFSLAHFGIVIKKIFFSSVVSILLILGI
                     QSLKKK"
     gene            8648..10528
                     /locus_tag="PLM04_03750"
     CDS             8648..10528
                     /locus_tag="PLM04_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HON02225.1"
                     /translation="MINDSLQNRRYVIAGIISVVIALYIIRLFSIQVIETKYKQGAVS
                     NAFLNKTLYPPRGLIYDRNQNLLVYNKPAYDIAVIMREIHDLDTLAFCRALNIDQTFF
                     NKRMAEIKDRKKNLGYSAYTPQLFLTQLDIQDIATIQQMMYKFPGFYIQSRTLREYAY
                     PYAAHVLGSIGEISQSRLDEDNYYVRGDYVGRDGLEYTYEKDLRGEKGVEVLLRDAKG
                     RIKGKYENGKHDVAPKTGKNLITTLDIQLQMLGEQLLNGKIGGIVAIEPKTGEILAMV
                     SSPTFNPSSLVGRQRTKNYIKLANDPAKPLLNRATQAQYSPGSTFKTIQALVCQQMGG
                     IDTKTLFPCNGPASSPIKCTHHHGSPVNLLNAIEQSCNPYFWGAFRTTLEKDGYGEKN
                     KNFKRNYQIWRSYVLSFGFGKKFTDGDIYQQSSGNIPTEKYFNRYFGETGWRAMTIRS
                     LAIGQGEILVTPLQLSNAMAIIANKGYFVTPHLNKADSLLNRVHRTSVEKNYFDIVDE
                     GMWRVCEFGTARHYKVPNLSMCGKTGTVQNSRGKDHSIFVGYAPRENPQIAIAVVVEN
                     AGFGATWALPIASLMIEQYINGKIERTDLLERIASATLSASATRLTNKTNLTDFINGQ
                     DF"
     gene            10512..11960
                     /gene="rodA"
                     /locus_tag="PLM04_03755"
     CDS             10512..11960
                     /gene="rodA"
                     /locus_tag="PLM04_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HON02226.1"
                     /translation="MARTSNIKYDWITIIYYVLLVFMGWLSIYGASYDFEHPAIFDFS
                     QRAGKQFIWILTAFGIAGILLLIDDRIYNYFSYFIYGFTILLLIATIFVAQDIKGSRS
                     WIVIGPISFQPAEIAKMSTSLALAKFMNTYGFKLRSWKNLIPLLVIVFIPLILIMLQN
                     ETGSALVFLSFFLMFYREGMNGIVLLIGALAAFFFVVVIRFSIVPIAFGTGTLGIVLA
                     MTLIIVGQFIYAYFFVKNKKEALYLLGGSSLVALVAYLLSLKFKVNFNSVAFFTVLAS
                     CVYWMVMELKFRHKKFLYLSLISLGFSLFSFSSDYIFEKTLEPHQQTRIKVLLNMEND
                     PMGAGYNINQSKIAIGSGGFWGKGFLNGTQTKLKYVPEQDTDFIFCTVGEEHGFVGST
                     IVLILYWLFLMRLISIAERQRETFNRVYAYCVVSIFFFHIMVNIGMVLGIMPVIGIPL
                     PFFSYGGSSLWGFTILLFILLRLDASRFEHIH"
     gene            12060..12800
                     /locus_tag="PLM04_03760"
     CDS             12060..12800
                     /locus_tag="PLM04_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON02227.1"
                     /translation="MKKLIIISLPLILLLFSMKASSQKFEKDVFPTSAGNLTITFIGH
                     GTLMFEIDNKVIHIDPTAMFADYSTLPKADLILITHEHADHFDMATIDKISKKETEII
                     LTQTCKNQLQKGKVMKNGDTYNWKKFIHIQAVPAYNIQHKTNTGQPFHPKGQGNGYIL
                     TFGNFKVYVAGDTENIPEMKSLKDIDVAFLPMNLPYTMTPKMTADAALSFMPKILYPY
                     HYGNTNTNELVNLLKNRPEIEVRIRKLN"
     gene            12870..13565
                     /locus_tag="PLM04_03765"
     CDS             12870..13565
                     /locus_tag="PLM04_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HON02228.1"
                     /translation="MKNRIVEAAIIAVGIICLGLCIRNGIKIFSQKDRAVEVKGLAEM
                     EVPANKVICPILYKDLGNDLAQLYVDMNKKNQAIISFIKENGIQENEISPSAPEIVDM
                     AAERFIENKSPYRYNLTSVITISTPNVDKVLALMSRQAELLKQGIAVTVNNYSVRYEY
                     TKLNEIKPQMIEEATKNARKAAEKFAKDSGSKLGKIKSATQGQFIISDRDENTPYIKN
                     VRVVSTITYYLED"
     gene            complement(13881..14555)
                     /locus_tag="PLM04_03770"
     CDS             complement(13881..14555)
                     /locus_tag="PLM04_03770"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HON02229.1"
                     /translation="MKSLTLSFIALFGAMVMAFGQFQLPKLDYGYSDLEPYIDSTTMY
                     IHYNNHHATYANNLNKTLEKYPELYKKDIVELLENINELPADIQLSVRNNGGGYYNHC
                     LFWSMLAPAGSTTISPKLEKILIKNFGSIDTFKNEFEKAALGQFGSGWAWLIKESSGK
                     LRIISTSNQDNTLMPFSSVKGKPVLTLDVWEHAYYLKYQSKRASYVKAFWNVVNWKEV
                     EKLIEK"
     gene            14826..15416
                     /gene="ruvA"
                     /locus_tag="PLM04_03775"
     CDS             14826..15416
                     /gene="ruvA"
                     /locus_tag="PLM04_03775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HON02230.1"
                     /translation="MIDYIKGQIVELTPTYVVLEANGLGYFISIPLPAYTLLSHQSEA
                     KLFVHEVVREDAYLFFGFITKKEREVFQLLISVSGIGVNTARMIMSSYSVEEIQEIIA
                     AGNVSALNAIKGIGSKTAQRIIVDLKDKILKGKDDGGSLSLLSDKNAQIKEEAVSALV
                     MLGFPVGASQKAIENILKEQSQLEVEQLIKMALKIL"
     gene            15496..22929
                     /gene="sprA"
                     /locus_tag="PLM04_03780"
     CDS             15496..22929
                     /gene="sprA"
                     /locus_tag="PLM04_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HON02231.1"
                     /translation="MTNRKKSSSIFVYFGIFLSSTVGFVYASLQQPVPAETRSNAVKQ
                     QDSVQQHFPVKKLVQDTYEDITAAYPLDAPVPSNIKTEIEYDPSTASYILHTYLGNIE
                     IATPFRMTEQEYLDYSGRQEMQRYWYEKNSSAQQNVEDKFSVTDMKFDIGPADKIFGP
                     GGVQIRTQGSAEISMGFKTNKIDNPALTERMRKSTILDFDEKIQMNVNGKVGDKVNFN
                     MNYNTESSFDFDQKLLKLNYKGKEDDIIKNIEIGNVSMPLNSSLITGSTALFGVKTDL
                     QFGKLSISAVASQQQSQTQTVSSKRGAQTTDYEIGIDDYDENRHFFLGHYFRNNFENA
                     MSKLPFVTSGVTITRVEVWVTNKRGNYDQARNIVAFMDLGETQRLDNHYWNVLSSTSL
                     PQNKANSLYDEVTSIPNVRDIRQTNNVLSSQYAADSIFGGEDYEKIESARRLDPSEYT
                     LNSSLGYISLRTALNPDEVLAVAYEYTYGGQVYQVGEFSTDAVTAPNALIVKLLKSTA
                     QSPSLATWDLMMKNVYNLGALQIQPENFQLDIVYRNDSVGTDLKYITEGNIQNQLLLR
                     VMNLDRLNTKNAAQPDGKFDYVEGYTVLSSSGRIIFPVLEPFGSHLRKAIGNDQIAEK
                     YVFQELYDSTLVAAREYSEKNKFRIVGEYKASSGNEIRLNAMNIPRGSVTVTAGGVTL
                     VENVDYTVDYTMGTVNIINTSILESGSNIDVKLENQSMFSMQRKSLVGTHLEYQFNRD
                     FSLGATVMHLSEMPLTTKVNTGDEPISNTIWGLNTSWRKESQWLTNQLDKLPFVNATK
                     PSTLALNAEFAQLIPGHSNVISSAGLAYIDDFESTKTTIDLHYPVFWHLGSTPFDPNG
                     GLFPEAALSNNVDYGKNRALLAWYYVDPILNANSRNTPENLRNNLESQSNHLTRNVLE
                     EEIFPNKERLATQTSRMTIMNLSFYPNERGPYNLDWEGINEDGSLKNPEKRWGGIMRK
                     LDVTDFENSNIEYIEFWMMDPFITDKDGTQKGGDLYINLGDISEDILKDGKKFFEQGL
                     SIDGDVTKSDTTVWGRVPKTQSTVKAFDNTPGARKYQDVGLNGLSTDEEFEYPTYKKY
                     VEQITRKISPQTLQKWQDDQFSPLNDPAGDNYHFYRGSDYDEQQKDILSRYKHYNGTE
                     GNSPEAENVNESYTTSATSLPDMEDLNEDNTMNEYEKYYQYRVQIRRDAFQVGKNYIA
                     DKITSNVKLANGNVEPVTWYQFRIPIREYNDKIGSIRNFKSIRFIRMFLTDFEKDIHL
                     RFATFDLVRGEWRSYTKSLYPNGLTPPSEGKLEVQAVNIEENADRTPVNYVLPPGVTR
                     ETDPGQPQLLQLNEQSMVLRVRDLAPNDAKAVYKTTSYDMRQYKRLQMFVHAEKLIDD
                     ISNLQDNELTCFIRIGTDMVNNYYEYEIPLRLTPHGKYLGENTEQREIVWYPENMFDF
                     AFSKLINVKLQRNKTLQASAGNVSNLVPYTIFDPDKPKNRITVVGNPTISDVQNIMIG
                     VRNRSGSIKSGEIWVNELRMSEFDESGGWAAMGNLAVGLSDIGTLNLSGRIETSGYGS
                     LESNVMNRNLEDLRQMNFAAAIELGRFLPERAKLQIPTYFSYSNETVSPKYNPLDQDV
                     ELSDALDNLPTKSDRDSLLNLSQTVSTSKSFNVANAKLNIKSKKPQFYDPANLKFTYA
                     YIENNEHNSEIEQNLIKNQRAAVDYNFNFAAQPWEPFKDVKALNSPAFKIIKDFNLYY
                     LPSSLNYNANLNRSYSQIKLRSFDVYSGSYTNDELSNLTFSKDFMFNRQFDIKYDLTR
                     SLHFSFQTVMNANIDEPYFTPEMGKEYYEAWRDTVWHNIKKLGTPYVYQQVFTASWNV
                     PLNKIPITDWMQANVSYNSNYNWNRSAQMEGGSNIGNTATSTGSWQFDGQLNFETLYN
                     KSKFLKNVLNRNKVTSARPQPFKPGNYTQTVQLKSDQPLTIQHRLGSERIRLTAKDKD
                     GNSVLLSYKVKNASSVEILPKQNMDSITINIVSLDPNQQSVVNKTRDITLGALMMVRR
                     FSINYRENNSTVLSGFMPETGFMGQQNYNGIDAPGYAFAFGFQEKDFIKKAFERGWLN
                     AGDSVVNPATTAFTSDLDIKATLEPIPGFKIDLNGRRYTASNTSVFYMYDGMPTTYSG
                     SFNITQIGLSTAFKKLGNATDNYNSETFNAFLANRQKVADRLNQKYVGTRYPQSGFIK
                     SSNLAGKEYDPSLGAYQLNSADVLIPAFLAAYTGRDATKISTNPFLSISSILPNWRIS
                     YDGLSKIEWFKNHFRNVNLTHAYSFRYSIGSYSSYSTWVPMEDESSLGYVRDVQTDNP
                     IPSGAYDFTSVSLNEQFSPLLGINATMNNSLSAKIEYRKQRNLALNLSSTQLIESASD
                     EYVLGFGYLLKDFDVILRLKSDKQSKIKNDLKLSADISYKDIKTLLRKVEENITQASS
                     GNKMITIKFLADYVFSSKVNIQLYYDRQSSTPLVSSSFPVSSSNFGASLRFMLTR"
     gene            23042..25042
                     /locus_tag="PLM04_03785"
     CDS             23042..25042
                     /locus_tag="PLM04_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HON02232.1"
                     /translation="MKKRFFLVLCCFFGFLFSEAQIFEPIHWDISHKLTGKTTADIIF
                     KASIDEGWHLYGLSLPPNGPKPTAIVFEKIENARKVGELQAVSNLEKKFDPNFGMELN
                     WFTKEAVFIQKITFSSKQNVRLEGYVEYMACNDKSCLPPTSEPFSIRLSTTEFNSINS
                     GEQAPSSESEKIQPTEDFKNAKQVDLSSEDTAVTSSEWWTPVVKELNSFGNVVYHKET
                     TSLWYILLAGLLGGFLALLTPCVWPIIPMTVSFFLKRSAVPSRGRKDAVLYGLSIIVI
                     YVTLGLLITLIFGASALNSMSTNAIFNLFFFVLLVVFALSFFGAFELTLPSSWSTKVD
                     AKSESTVGLLSILLMAFTLVLVSFSCTGPIIGTLLVEVASTGSLLAPAVGMFGFAFAL
                     AVPFTLFAFFPSWLESLPRSGGWLNRVKVVLAFIELAFALKFLSVADLAYGWHILDRE
                     VFVSLWIIIFALLGIYLLGKIRFPHDSESKHTSVGGLMLGIISLAFAVYMVPGLWGAP
                     LKSISAFAPPLYTQDFNLYNNEVHPAFTDFEEGMAYAAMKKLPVIIDFSGYGCVNCRK
                     MEASVWTDPQVKALLDKNFVLISLFVDDKTPLEKPFEINENGKTRKIKTVGDKWSYLQ
                     RYKFGANAQPFYVMIDYHGKPLNKPYVFDTNPSHFVNWLNRK"
     gene            25309..25890
                     /locus_tag="PLM04_03790"
     CDS             25309..25890
                     /locus_tag="PLM04_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HON02233.1"
                     /translation="MEKENKASEKNQSAENEKKHQQQHKENEDNIIPQSENSEAENTE
                     KELERLREECEDLADKNLRLLAEFDNYRKRMLKEKADLLKNGGENVLVNILPLVDDFE
                     RALKAIETSDDIEAVKEGIHLIYGKFVDFLNKNNVKEIPTENVEFNTQYHEAVSKIPA
                     PSEELKGKVIECLSKGYTLNDKVIRYAKVVVGE"
     gene            25964..27115
                     /gene="dnaJ"
                     /locus_tag="PLM04_03795"
     CDS             25964..27115
                     /gene="dnaJ"
                     /locus_tag="PLM04_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HON02234.1"
                     /translation="MAKRDYYEILGVSRTATEVEIKKAYRQKAIQFHPDKNPGDKKAE
                     ENFKEAAEAYEVLSDPEKRERYDRYGHAGVGSSASSGSYGGGMTMEDIFSHFGDIFGG
                     HFGGFSGFGGGSRSSAPRMRRGTDLRVKVKLTLAEIAEGVEKKIKVKKYVPCSHCQGT
                     GAAEGSAPVTCSMCRGTGRVTRVQQTILGQMQTTSDCPTCHGEGKVIKEKCIHCNGEG
                     IIHDEEIVTINIPAGVAEGMQLTLSGKGNAARRGGINGDLIVVIEEEPHSELIRDGND
                     LIYNLLLSVPQAALGGAVEVPTVNGKVKVNIDAGTQPGKVLRLRGKGLPDVNRYGNGD
                     LLINIGVYIPEKLSKEEKTIMEKLEQSSNFKPSSSAQKGFFSRFKDMFD"
     gene            complement(27343..>30179)
                     /locus_tag="PLM04_03800"
     CDS             complement(27343..>30179)
                     /locus_tag="PLM04_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HON02235.1"
                     /translation="FVFSFVFPQFTKAVPAYPFPVKVVQPDGTEISVKLNGDEFFHYQ
                     TTLDGYLITRDKDGFFKYGKLSKKKEIAPSKYRVNNIEKRTKEEINFIKQLPKGIDFS
                     KQNQLNRIQRASSVTVTEKSGRKAYPLTGSPKSLVILVNFSDNSFTVSSPKEAFTKLL
                     NQNGYSENGSTGSARDYFYDTSTEKFNPQFDVVGPYTLPQTMGYYGENGSDGMDKNPQ
                     QMIIDACRLADNDGVDFSQYDTDRDGYVDNIFVYYAGYNEAEGAPANTIWPHRWTLAN
                     YNTKFDGVVVFDYACSSELRSSRGANMCGIGTFCHEFGHVLGLADYYPTDGGTHFTLS
                     YWNIMDAGAYLNDGKTPPTYSAFDRFFLNWLTPIELKSSGNFTLQPLTSSNSAYLISK
                     DGNHNLVGNSPNPSEFFMLENRQKSGWDAYLPGHGLLVTHINFNQSDWENNTPNNIAG
                     KLGYKIVEADGIGSEQTLAGDPFPGINNITTCSLVLRNGTKLKKSLTNITENNYINLD
                     FMGEENSPIVGAMSNFLAFETVQGTPSNAQLLKISGKKLKENILLAFSENKHFEMQLE
                     NDFNAEWTKNLILAPIDSVVDTTRILVRYNPTEPSYLDVHNEIISITSKDATPIYLNL
                     QGKSTRPVYVVPPVATEATDVSISSYVAHWEPVTDATGYYFTAYYISNESSETTEGFD
                     NGLTPSSGWTITAQALSTSASYSGKEVPAIQFKNSGEMIQTENYVLPAIKLSFFVKSF
                     SGNGALKVEAKNDSDKWITIDNVSVTTSLSAIKTYTFTESDHYTQFRLSYQKESGYLI
                     IDDITVGFAKKINYNEKDKWITSTTDTVINLPSNSDCYYKVKASDKTLRNSVILYENI
                     TDFSNLIYVHTLPDVGKQLKAYVNREDKTVTITVPNTSTVVRIYDVTGKLIVSEIPRY
                     NKFTIDALKNRIPGQLYIIVAGDRTAKIIM"
BASE COUNT         9377 a         5595 c         6051 g         9156 t
ORIGIN      
        1 cctttccaaa cgtcggtgag atattcatca taagcattct ttccgccaat aataaatatc
       61 cagttggaag attcgatttc ctgctttgag gcagtagagc tgtaagtacg tggccgataa
      121 actacgactg aagaataatg acgtattccc atttctttgg gaagattatt gagattactg
      181 tccgggactt gccaactgaa accctgatcg acagattgac ggaaataact tcccggaatt
      241 ggtttgttat cctgtccgaa aagtaagagt ttatcatcgt aatttattac cgaagctccc
      301 ctccctatag aatcaagcga agcattctcg gcgctgaagt caacccagta aatgccatct
      361 tctgtgttcc aatttgtttt caaagcagtt ccatcggaag aaaaaccggt caacaccagt
      421 cctttctgct tgttcacttt cgtattgaat accactgagg caaatccacg aaccggaaaa
      481 ttttcaggaa tttcattgta aatcagccat tctgtaccat cgacagaaga agcaaaccga
      541 agttttccat ctgtcaaaga atgggaaaca gcccaaagcc gatcgtttaa aacaaaaatc
      601 agtgaagtaa attcgtattc ggaataagtc tgtcttgtcc atgtcaatcc atcacccgaa
      661 gaaaaaatgt tcgaattccc gcatgttaca taaagctgat tgttgaaaac tgtaatatcc
      721 tcaaaagttg catttgtagg aagtcccgaa ataacatctt gagtccatga taccccattg
      781 ggagaaacaa acaagtaatt ctgctggcgg tcgtttacaa aaagatacaa ccgatcgttg
      841 aaataaacag ccttttgatg ttgaacattt aaagagtgag ccgcatcgtt taccttttgc
      901 caaatataca attccggttc cacttgatga acatttacct tgatcagata tttgcgcctt
      961 gttactccat cagaagcaat attaataatt gccttaatct ttgtaaagtc gaaagcttca
     1021 tttccggaaa taagggctgt tgaatcattt tcataaacca aataggcagc agacgtgctt
     1081 ttaaaggtga aagtggcata aacactgtca attcttgtct ggtagggtag cgaatcaagg
     1141 ttgacaatca ccgaatcgta ttggagacta aaaacagcat cttttagatt gggtatctta
     1201 ttgttggccg aaaaagtaag cgaaacaaaa cttggatcag aagaaatttc ctttacatta
     1261 ccatccccca agcaggaatt taggaaaaaa atagtaccta acaaagcaag tattttcaat
     1321 tgatttcgca gcatgaacaa taagttttga atgaaggagc aaagttaaaa aacatttcag
     1381 ttgttgcgac gaaaaggtcg atattttatt gttttttatt actttagcgg ataagtatta
     1441 cttaaaatta attaaacatc gagttccacc atgacaggac aatgatcgga atgaaaagct
     1501 tcgctaagga tagcagccgc tttcagtcga gacctcagcg gttcgcttac ccaatgataa
     1561 tcgatgcgcc agccggcatt tctgtaacgc gcaccaccac gataactcca ccagctatat
     1621 ttttccggac tctggtcgaa aacccgaaac gaatctacca ttcccaaagc ctgaaatcga
     1681 tccatccatt cccgctcttc gggtagaaac cctgatactc ctatttgacg ttcgggatga
     1741 ttaatgtcaa tgggcttgtg gcagatgttg taatcgccac aaaccaccaa attgggtctt
     1801 tcacgaagca agttttgtat aaatttcaga aattcttcta gaaatttcat tttgaaatcc
     1861 tgtctttctc cgcctgatga tccggaagga acatatacat tgataaccgt tatatcgcca
     1921 aagtctgtcc ttaatactct tccttcagtg tcaaattcgg gattatccat tccgaaataa
     1981 acataatcgg gcggaatttt agtaagagta gcaacgccac tgtacccttt tttttgtgcc
     2041 gaatgaagat aagcggtata acccagttcg gcaaaaagta gggtgtcgat ttggtcgggc
     2101 tgtgcttttg tttcctgcag acaaaggaca tcaggattct ccgcttgcag ccattgaata
     2161 aaatctttgg tcagtgcagc tcttaaaccg tttacattgt atgagataat tttcatggat
     2221 attgcttgct tgaggttgaa attttcagca aatgaattaa tagggatttt gttttaacat
     2281 ttttaactgt ttaaataact tggatgcgaa aacaaagtta ttgaagcttt cattctcagc
     2341 atcaaaaatt tcatcttttg tgccttccca agctttcact cctttttcga tataaatgat
     2401 tttttcgcca atttccatta ccgaattcat gtcgtgtgag ttgataatgg tggtaatatt
     2461 gaattcgtgg gtaatttcat aaatcaaatc gtcaataatc atagaggtaa ggggatcgag
     2521 cccggaattg ggttcatcac aaaaaagata tttaggccgt aaagcaatgg cacgcgcaat
     2581 ccccacccgt ttttgcatcc cgccactgat ttcggaagga taaagatcgt aagatctttc
     2641 ttcgaggtta acgcggcgaa ggcaaaaata agctctttcc cttatttcct cattactttc
     2701 atgggcaaac atttccagcg gatacattac attttccagc accgtcatgg aatcgaacag
     2761 ggcagctccc tgaaaaagca tgcccacttc acgtcgcagc ataagtattt cttgaggctt
     2821 catttttggt aaatttcgtc catcgtactc tatacaacca ctgtcgattt ggtgaaggcc
     2881 gataatacat ttcatcaaaa ccgttttgcc cgaaccactt tgtccgatga tcatatttac
     2941 cttgccggtt tcaaaaacgg cagaaatgtc ctgcaatgcc ggaacgccat caaaagtttt
     3001 tattatgttt tttacttcaa tcatgcttgg tttgaagttg gtgtgttatt cattaatatt
     3061 tatcctcctt tttaggtaaa cattgtgttt ttaccaggaa aaatcgtttc attttagaat
     3121 ttctgctgaa gattgaaagc gaaaaaagta ttttttatcc caacattaca ttggtaatca
     3181 atagattaaa aaacaaaatt aagatactgc tgttaactac ggcgttggta cttgctttac
     3241 ccacttccaa agctcctccg taagcataat aaccataata ggaagaaacg gaagaaatga
     3301 tgaaagcaaa aaccagcgat ttaaccattg cataaaacac ataaaaaggg ttaaaggcat
     3361 attggatacc cagcagataa tcggccaccg ttatgacatc gctgaaaacg ccgaccaaat
     3421 aaccgccaat tagtcccaca gccatgctga aaattaccaa aaacggcatc ataacgacga
     3481 aagccacaat ttttggaaga ataagataat tggcagaatt gacgcccata atttcgagtg
     3541 catcaatttg ttcggtaata cgcatggtac caatttcgga agcgatattg gaaccgactt
     3601 tgccggccag aatcagacac atgatggtag aagaaaattc gagcagtaat gtatcgcggg
     3661 ttaccaatcc agtgctatag gttggaagta agggattttc cgtattgagt ttggtttgaa
     3721 tggtcatgat agccccgatg aatacagaaa tgattaatac tatgggcaaa gattgtaaac
     3781 ccagtttatc caattctttg ggaaattggc ggaaaaacat tttcgaacga tcaggctgta
     3841 caaacacacg tttcatcagc aaaaaatatt ttcccgtttc ttgaaataaa ttcatacgca
     3901 aattttcaaa atttcaagca gttaaaaaat tgtcgacaaa ccatccaaca agttttgaag
     3961 caaaaagttc ttattttgtt tcgcaaacaa gttgcaaata tacttaattt ttgcaattga
     4021 tgaattagta cactactatt caaaagcaaa aaagaatgtg tgagaatttc ttattttagc
     4081 gataaaatca aaataaacag aataataaaa tttcacaggc ttcagtaaaa aaataaacag
     4141 aatcttaatc tttacgataa agcatggatt tatttacttg atagcctgtc tcgtgtcttt
     4201 cggttattca tgcaatatta accgaaggtg caaaagtaga tatattcaaa aattattttg
     4261 ataagaataa gtttgaaatg ggtcaggatt atttatgtat caataaaata gaagcaaatc
     4321 gtgtaagaag ggctcgtttc caaaaaattt ctgattgggg ataaagtatg gaataaaatt
     4381 aaaaagttcg atcattaggg actaatttta ctttgtcttt ttgtttgaag atttgcaaaa
     4441 gcaactttta aaacacaata agatttttta atagtttttc tgtaaaagtt atggtgtcaa
     4501 gaacaaattt acaaggaaaa atttcgattt taccttgtct gtgtgtaaat tgaaaatttt
     4561 cgtgtaactt tgcatcccat aaccaaagta acaaaggcat ggaaaaaaag gtaatcaaaa
     4621 aagcattaat atcggtctat cacaaagaca atctggaaga aataatcacc aaactgcatg
     4681 ctgctggagt agaatttatt tctaccggag gcacaaaatc gtttattgaa tcgctggaca
     4741 ttccttgcga agcagtagaa gatttgaccg gctacccttc aattcttgga ggaagagtaa
     4801 aaacactgca tccgaaaatc tttggaggta ttctcgcgcg gcgggacaat gctcaggatg
     4861 tgcagcaaat gtctgaatat gaaatttcgg gaatcgattt ggtcattgta gatttgtatc
     4921 ccttcgaagc cactgttgcg gcaggtgcag atgaaccaac aaccattgaa aaaattgata
     4981 ttggtggaat ttcgcttatc cgtgctgcag caaaaaattt caaggatgtg gttgtcatcg
     5041 cctccaaaga gcaatacgaa ccttttctgg aaattttaaa cagcagtgga gctcaaactt
     5101 ccatcgagga acgccgatgg tttgccaaag aagcttttgc tgtttcctct cagtacgata
     5161 cagctatttt caactatttc gatgaacctt atcaaagtgc ttttcgctgg tatgaaaatc
     5221 atccaaaagt tttacgttat ggcgaaaatc cgcatcaacg cggaattttc tttggcaaat
     5281 tcagcgaaat gtttgaacaa cttcaaggaa aggaaatttc ctacaataat ctgctcgaca
     5341 ttgacgccgc tgtaaatctt atcaatgaat ttgacgacat aacctttgct gttctcaagc
     5401 acaacaatgc ctgcggaatt gcatcacgtc cttcacttct tgaagcatgg aaagctgcgc
     5461 tggctggcga tccggtttct gctttcggcg gaattctcat caccaatgcc atcattgaca
     5521 aagaaacagc tctggaaatc aacaaaatat tttttgaagt aatcattgct cccgattatg
     5581 atttggaagc cattgaaata ttaagtcaaa agaaaaatcg gattattttg ataatgaaaa
     5641 acaatacact gcgtcccaag caattccgtt ccttactgaa tggagtgctg atgcaggata
     5701 gagacttgca taccgaaacg gctgctgact taaaggtagt taccgaaaaa tcgccttcag
     5761 aagctgaaat tgaagattta ctttttgcaa ataaaattgt aaaacattca aaatcaaatg
     5821 ccattgtact ggcgaaaaac aaacaattgt gtgccagtgg tgtcggacaa acttcgagag
     5881 tggatgcatt ggttcatgcc attgacaagg cgcgttcttt cgatttcgac ttaaaaggtg
     5941 ccgtcatggc ttccgatgcc tttttcccgt ttcccgactg tgttcaaata gcacatgaag
     6001 caggaatcac tgccgtcatt caaccgggtg gttccatcaa agaccaggac tccatcgatt
     6061 tctgcaatca aaagggtctt tcgatggtat ttacaggtta tcggcatttc aaacattgac
     6121 aatttgcaaa taaaaatttt cttaaaaaca taaacacaca aacaaatggg ttttttttca
     6181 tttacacagg aaatagccat cgatttaggt actgccaaca gcattatcgt ttacaatgat
     6241 cagatagtgg tggacgaacc gtctgttgtg gctttggaca aacgtactga ccgattgata
     6301 gccgtaggag aacgtgcacg ccagatgcag ggaaaggaaa acgagtcgat tcgtaccata
     6361 cggcctttac gcgatggggt aatagccgat ttttatgctg ccgaaatgat gattcgggga
     6421 cttatcaaaa tgattccttc aaaaaatcgt tttttcactc cttccttaaa aatggtcatt
     6481 ggtatcccat caggcagcac tgaagtagaa atgagagccg tcagggattc ttcagaacat
     6541 gcgggagggc gcgaaatata tatgatttac gaaccaatgg cagccgcttt gggaattgga
     6601 atcgacgtgg aagctcccga cggctgcatg gtggtagata ttggtggagg aacaacagaa
     6661 atagccgtaa tttcattggg cggaattgtt ttgaacaagt ctattcgcat tgccggagac
     6721 gatctgaccg acgatattgt ggaatatatg ggacgaatgc acaatattaa aattggagaa
     6781 cgtaccgccg aattgataaa aattaatgtt ggagctgcat tgaccgattt ggaagatgct
     6841 ccggaagact atattgttcg cggaccaaac agaatgacgg cattgcccat tgaagttcct
     6901 gtatcttatc aggaaattgc acattgtctc gaaaaatcaa tttcaaaaat tgaagcagca
     6961 atattaagcg cattggaact gacgcctccc gaactctatt ccgacattgt tcagcatggt
     7021 atttttcttt cgggtggcgg agctttgctg cgtggattag acaaaagact cacggataaa
     7081 atcaacattc cttttcatat tgccgatgac ccgcttcatg cagtagcacg cggcaccgga
     7141 atagctttga aaaatgtcga taaattctct ttcttgttaa gataaaattg ttaatattcg
     7201 attctgccgg actacaagaa tgtattttct tactgctcca aagaatcgga tatttttgtg
     7261 tttgcagaaa aaagccgcaa agattacaaa ctttttatga aaaatctcat taattttttg
     7321 attcatcata gcggattttt tgtattcttg ctgttggaaa tcctttcact gatgctggtt
     7381 ttcaataaca gagattatca gaaaagtgta tttttttctt ccagcaacac cataatttct
     7441 tctttttaca actggaacaa ttatattgtc ggctttttca atttaagcaa atcgaatcag
     7501 caactggccg aagaaaacac acaactcaaa aatcagttgc tcgacttgca aaataaactg
     7561 gaagcgttgc aaccgtcagg gactgattcg ctttcgtaca aaatccctcc ggaaaagcaa
     7621 taccgattta ttgaagcaaa agtgatcaac aattctacca ataaactgca aaattatatt
     7681 acgttgaaca agggagcttt ggatggcgtg aaaccggata tgggagtaat caacgacgaa
     7741 ggcatagtag gtattgtaaa aaatgtttct cagcattttt ccgttgtaat tccggtttta
     7801 aatcccaaaa ttcaaatcaa ttgcaaattt aagcgaaaca attacattgg ttcgcttgta
     7861 tgggatggaa aagattatcg ctttgccttg ttgaccgata tagcgcgaca tgttccgata
     7921 aaaatgggag acacattggt taccagcgga ctgacgacta ctttccccga aggtattccg
     7981 gtaggcacca tagaaaattt taacctcaag gaaagcgatg cctattatga aatcaaagtg
     8041 aagctggcaa caaattttcg taccctttcc tatgttaagg tgattgattt caaaaatgcc
     8101 ggcgaattaa aagagcttga aaaagcagca tacgaagaat aagatggcaa ttacggtttt
     8161 tcaaaatatt attcgtttcg ttttactgat gatggttcag attttcgtac tgaacaacat
     8221 tcagttaagt gggtacatca atccctatat ttacattctt ttcattcttt cgttgcccta
     8281 tcgcacacct cagtggttaa ccttgattct tgcttttgta accggactca tggtggatat
     8341 ttttatgaac acaatgggaa cacatgcttt tgcaactgtt ttaatggctt tcaccagaaa
     8401 cggaatcatc aatttgatta ccgatttcga aaaagatgaa aatcctactc catctttcta
     8461 tacttttggc attggcgtgt atatcagata tattgtattg cttgttttca tacatcatac
     8521 cgcccttttt tttctggaag cattttcact agctcacttt ggcattgtta tcaaaaaaat
     8581 tttttttagc tcagttgtat caattttgct aattttgggc attcaatcgc tgaagaaaaa
     8641 ataatcaatg ataaacgatt ctttacagaa cagaagatac gtaattgcag gcatcatttc
     8701 tgtagtcatt gccctgtaca tcattcgttt gttctctatt caggtaatag aaacaaaata
     8761 taaacaggga gcagtcagca atgcatttct gaataaaaca ctttatccgc ctagaggttt
     8821 aatttatgac agaaatcaaa acttgctggt ttacaacaaa cctgcctatg acatagccgt
     8881 catcatgcgt gaaattcacg acttggacac gctggcattt tgtcgtgctc tgaatataga
     8941 ccaaacgttt ttcaataaac gaatggccga aatcaaggac agaaaaaaga atcttggcta
     9001 ttctgcctat accccgcaat tgtttttgac tcaactcgat attcaggaca tagctaccat
     9061 tcagcaaatg atgtacaaat ttccgggatt ttacatccaa agccgaactt tgcgtgaata
     9121 tgcctatcct tatgccgcac atgttttggg aagtattgga gaaatttcac aatcgcgatt
     9181 ggatgaagac aactattatg ttagaggcga ttatgttgga agagatgggt tggaatacac
     9241 ctacgaaaaa gatctcagag gagaaaaagg agttgaagtc ttgctgcgtg atgcaaaagg
     9301 cagaataaag ggaaaatacg aaaacgggaa acacgatgtg gcacccaaaa cagggaaaaa
     9361 tcttattacg acgcttgata tacagcttca aatgctggga gaacagttgt tgaacgggaa
     9421 aataggtgga attgttgcca tcgaacccaa aaccggcgaa atattggcta tggtttccag
     9481 tcctactttt aatccttctt cacttgtggg aaggcaacga acaaaaaatt acataaaact
     9541 ggcaaatgac cctgccaaac ctttgttgaa tagggctaca caagcgcaat actcacccgg
     9601 ttctaccttt aaaactattc aggcactggt atgtcagcaa atgggaggaa tcgatacgaa
     9661 aacgcttttc ccttgtaatg gacctgcatc ctctcccatc aaatgcacgc atcatcatgg
     9721 ttcaccggtt aatttgctta atgctataga gcaaagttgc aatccttatt tttggggagc
     9781 tttccgcacc acattggaaa aagacggata cggcgaaaaa aacaaaaatt ttaagcgaaa
     9841 ttatcaaatc tggcgaagtt atgtattaag tttcggcttt ggaaaaaaat tcaccgatgg
     9901 cgacatctat cagcaatctt caggcaatat tccaaccgaa aaatatttca atcgctattt
     9961 tggggaaacc ggatggagag caatgaccat tcgatcatta gccattggtc agggtgaaat
    10021 tcttgttact cctcttcagt tgtcaaatgc tatggctatt attgccaaca agggttattt
    10081 cgttactcct catctcaaca aagctgactc gttgttaaat agagttcatc gtaccagcgt
    10141 ggaaaaaaat tatttcgaca tagtagatga aggaatgtgg cgggtttgcg aatttggaac
    10201 ggcacggcac tacaaagttc ccaatttgtc catgtgcgga aaaacaggaa ccgtacaaaa
    10261 cagtcgggga aaagatcatt ctatttttgt tggatatgcg ccaagagaaa atccccaaat
    10321 tgcaatagcc gttgtggtgg aaaatgcggg atttggagca acatgggcgc tcccaattgc
    10381 cagtttaatg attgagcaat atattaacgg aaaaatcgaa cgtaccgatt tgctggaacg
    10441 aattgcatcg gcaactctct cagcttccgc aactagatta acaaataaaa ccaatcttac
    10501 agattttatc aatggccagg acttctaata tcaaatacga ctggataacc atcatttact
    10561 acgttttgct ggtttttatg ggttggctta gcatttatgg cgcaagttac gacttcgaac
    10621 atccggcaat ttttgacttc agtcagcgtg cagggaaaca atttatttgg atactgacgg
    10681 ctttcggcat tgcaggcatt ctattgctaa ttgatgaccg aatttacaac tatttcagct
    10741 actttattta cggatttact attttattgc ttattgccac aatttttgtt gctcaggaca
    10801 tcaaaggttc gcgttcgtgg attgtcatag gtcctattag ctttcagcct gccgaaattg
    10861 caaaaatgtc cacatctctt gcgttggcca agtttatgaa cacatatggt ttcaaattgc
    10921 gatcgtggaa aaacttgatt cctttgttgg taatcgtatt tattcctttg attctcatta
    10981 tgctgcaaaa tgaaaccgga tcggctctgg tttttttgtc gttttttctg atgttctatc
    11041 gagagggaat gaatggaatt gttttattga ttggtgcttt agctgcattt ttctttgttg
    11101 tggttattcg tttcagcatc gttccaattg ccttcggcac aggcacattg ggaattgttt
    11161 tggctatgac gttaattatt gttggacagt ttatatatgc ttattttttt gttaaaaata
    11221 aaaaagaagc gctttatttg ttgggtggaa gttcattggt tgctttggtt gcctatctgc
    11281 ttagtctgaa atttaaagtg aatttcaatt cggtagcttt tttcacagtg ctggcttcct
    11341 gcgtatactg gatggtgatg gagctgaaat ttcgccataa aaagtttctt tatctttcgc
    11401 tgatttcact tggtttttca cttttcagtt tttcgtcaga ttatattttt gaaaagacac
    11461 tcgaaccgca tcaacaaacc cgtatcaaag ttttgctgaa tatggaaaat gaccctatgg
    11521 gagcgggtta taatatcaat cagtcgaaaa ttgccattgg ttcaggtggt ttttggggaa
    11581 aaggtttttt gaacggaacc caaaccaaat tgaaatacgt tcccgaacag gataccgatt
    11641 tcattttttg taccgttgga gaagaacatg gctttgtcgg ttccaccata gtacttattt
    11701 tatattggct ttttctgatg agacttataa gtattgccga acgacagcgg gaaactttca
    11761 acagggtata tgcatattgt gtagtgagta ttttcttttt ccatattatg gttaacattg
    11821 gtatggtact cggcatcatg cctgttattg ggattccttt gccatttttc agttatggtg
    11881 gctcttcact ttggggcttt acgattcttc tttttattct tttgcgactc gacgcttcaa
    11941 gatttgaaca tattcattga tacaaatcaa taatattttt tatatttacg ccgatattct
    12001 ggataattta ttgaattaac acttatattc gtttctgtta aatccattaa aaaaatatta
    12061 tgaaaaagtt aattatcatt tcattgccgc ttattttact tttattttct atgaaagcgt
    12121 catcccaaaa atttgaaaaa gacgtatttc ccacatcagc gggaaattta acaatcacgt
    12181 ttatcggaca tggaacattg atgtttgaaa tcgacaataa agttatacac attgacccca
    12241 ctgccatgtt tgccgattat tctactcttc ccaaagccga tttgatactt attacacatg
    12301 aacatgccga tcatttcgat atggctacca tcgacaaaat cagtaaaaag gaaaccgaaa
    12361 tcattcttac ccaaacctgc aaaaatcaac tgcaaaaagg aaaagttatg aaaaacggag
    12421 acacttacaa ttggaaaaaa tttattcata ttcaagctgt tccggcttac aacatccagc
    12481 acaagacaaa tacgggccaa cccttccatc ccaaagggca aggaaacggt tatatattaa
    12541 catttggaaa ttttaaagtt tatgtggccg gcgacaccga aaacatacct gaaatgaaat
    12601 ctttgaaaga tattgatgtg gcttttttac ccatgaacct gccctacaca atgacaccca
    12661 aaatgaccgc cgacgcggct cttagcttta tgccaaaaat tttatatccc tatcattacg
    12721 gtaataccaa cacaaacgaa ttggtaaatc tgctgaaaaa cagacccgaa attgaagtga
    12781 ggattcgcaa gttgaattaa gattgatttc taaagaactt attttttcta aacaaacaat
    12841 attgtttcac aaattaaaaa atcaattcca tgaaaaacag aatcgttgaa gcagccatta
    12901 ttgctgttgg aataatttgt ctgggactct gcatccgaaa cggcattaaa atattctcac
    12961 aaaaagaccg cgcagtggaa gtaaaaggac ttgcagaaat ggaagttcct gccaacaagg
    13021 tgatttgccc cattctttac aaggatttgg gaaatgacct tgctcagctt tatgtagata
    13081 tgaataaaaa gaatcaggcc attatttctt tcataaagga aaacggaatt caggaaaatg
    13141 aaatatcacc ttcggcaccg gaaattgtgg acatggcagc cgaaagattt atagaaaata
    13201 aatcgcctta tcgatacaat ttgacttcag ttattacaat tagcacacca aatgtggata
    13261 aggtgcttgc tttaatgtcg cgtcaagctg aacttctcaa gcaaggaata gctgttacgg
    13321 tcaacaatta cagcgttcga tacgaatata cgaaactgaa tgaaattaag ccccaaatga
    13381 tagaggaggc aacaaaaaac gctcggaaag cagccgaaaa atttgccaag gattcaggga
    13441 gcaaattagg aaaaattaaa agcgccactc aaggtcagtt tatcatttcc gacagagacg
    13501 agaacacgcc ttacataaaa aatgtccgcg ttgtttctac cataacctat tatttggaag
    13561 actaaaaaat tttttataat tatccgcatt gcgatatatt ataatgatta tccgcaatga
    13621 attttattgt aaattattga cttgaaatcg gagtgtagtt tacggcagca ataagcaatc
    13681 tgtagaagat aacttcttga aaataacgtt aattgaattt atctcagaac caatttgttt
    13741 actaaaactg ctaaaataga gtaaactgaa aagcgaaaaa atcgttgttc tcgaaaactt
    13801 gtgttattga cgacatgcac aaaaaaaggt aacgtttagc atattacctt tttttttaaa
    13861 aaaaatcaaa gtgaagaaat tcacttttca atcagttttt caacttcttt ccaatttacc
    13921 acattccaaa aggctttgac ataggatgct cgttttgact gatatttcag ataatacgca
    13981 tgttcccaca catcgagcgt caaaaccggc tttcctttta ccgaagaaaa tggcattaag
    14041 gtattatcct gattggaagt ggaaatgatt ctcaattttc ctgaagattc ttttatcagc
    14101 caggcccatc ctgaaccaaa ttgtcctaat gcagcttttt caaattcgtt cttaaaagta
    14161 tcgatgctgc cgaaattttt aatcagtatt ttttcgagtt tgggagaaat ggttgtggaa
    14221 ccggccggtg ccagcatcga ccagaaaaga caatgattgt aataacctcc accattgttg
    14281 cgtaccgaca attgaatatc agcaggcaat tcgtttatat tttccaacaa ttctacgata
    14341 tccttcttgt aaagttcagg atatttttcg agtgttttat tcaaattgtt tgcgtaagtc
    14401 gcatgatggt tgttgtagtg aatgtacatg gttgtactgt caatatacgg ttcaagatcg
    14461 ctgtagccat aatcaagttt tggaagctga aattgtccga aagccatgac catagctcca
    14521 aataatgcta tgaatgataa tgttaaggat ttcattttta taatgttttt agagttatta
    14581 tatttataaa caacagactt atttcctttg ttcagtgatg aaaaaaaatc tataaggttg
    14641 atgccatttt ttaccatcat ggaagtcggc ttttcttcct tttaacgttg taattgtaat
    14701 ttttaatcaa atacagcgtt attttaatca gtacttctgt caatgggttg gaggttgtct
    14761 tttggagcgt atgtaaataa atttgtattt ttacacacaa aaactctttt ttaaagtttt
    14821 gtcggatgat tgattatatt aaggggcaaa tagttgagtt gaccccaact tatgtggtgc
    14881 tggaggcaaa cggactggga tatttcattt caatcccttt gccggcctat actttattga
    14941 gtcatcaatc ggaagcaaag ctttttgtgc acgaagtagt gcgggaagat gcttatttgt
    15001 tttttggttt cattaccaaa aaggagagag aagtttttca acttttaatt tccgtttcgg
    15061 gcataggcgt aaatactgcc agaatgatta tgtcttctta ttcggtagaa gaaattcagg
    15121 aaataattgc cgccggcaat gtgtcggcgc tgaatgccat taaaggcatt ggcagcaaaa
    15181 cggcccagcg tattattgtg gatttgaagg acaaaatttt aaaagggaaa gatgatggtg
    15241 gttcgctgtc tttgctttcc gacaaaaatg cccaaataaa ggaagaggca gtgtcagcat
    15301 tggtcatgct tggttttccg gtcggtgctt cgcagaaagc aatagaaaat atcttgaaag
    15361 aacagtctca gcttgaagtt gaacaattga tcaaaatggc cctgaaaatt ttgtgagggt
    15421 ctgcaaaatt tgaaattcaa gaaaacttca tttcagtgaa gacaaattgt taagaacaag
    15481 gaatagaaat tttatatgac aaatagaaaa aaatcatctt cgatttttgt ttatttcggt
    15541 atttttttga gttcgacagt tggatttgtt tatgcttcgc tgcaacaacc cgttccggcc
    15601 gaaactcgtt ccaatgctgt gaaacagcaa gattccgttc agcaacattt tcctgtcaaa
    15661 aaattggtgc aggataccta cgaagatatc actgcagctt atcctctcga cgcacccgtt
    15721 ccttcaaata ttaaaacgga aattgaatac gacccctcaa cagccagtta tattttgcat
    15781 acttatttgg gaaacatcga aatagctact ccgtttcgaa tgacggaaca ggaatatctc
    15841 gattattccg gaaggcaaga aatgcaacgt tattggtacg aaaaaaattc ttctgcacaa
    15901 caaaacgttg aagacaaatt cagtgttacg gacatgaaat tcgatattgg ccctgccgat
    15961 aaaatatttg gtcccggtgg agttcaaatc agaacacaag gttctgccga aatttctatg
    16021 ggtttcaaaa ccaacaaaat cgacaatccg gcattgactg agcggatgcg caaaagtact
    16081 attctcgact ttgacgagaa aattcagatg aatgtaaatg gcaaggttgg cgacaaggtg
    16141 aacttcaata tgaattacaa taccgagtcg agtttcgatt ttgaccagaa attgttaaaa
    16201 ctgaattata aaggcaagga agatgatatc attaaaaaca ttgaaattgg taatgtcagc
    16261 atgccgttga acagttctct cattaccgga agcacggcat tgttcggtgt taaaaccgat
    16321 ttgcagtttg gcaagttaag cattagcgcc gtagcttctc agcaacaatc gcagacgcaa
    16381 accgtaagtt caaaaagagg agcacaaact accgattacg aaattggtat tgatgattat
    16441 gatgaaaacc ggcatttctt tttgggacac tatttcagaa acaattttga aaatgccatg
    16501 agcaagctcc catttgtaac ttcaggtgtt accattacac gtgttgaagt ttgggtaacc
    16561 aacaaacgtg gaaattacga tcaggcaaga aacattgttg catttatgga tttgggagaa
    16621 acacagcgac ttgacaatca ttattggaat gtgctttcat ccacatcttt accacaaaac
    16681 aaagccaatt ctctttatga tgaagttact tctattccta atgttcgcga catacggcag
    16741 accaacaatg tactttcttc tcaatatgcc gccgatagta tttttggagg agaagattac
    16801 gaaaaaattg aaagtgcccg acggctcgat ccttcggaat ataccttgaa ttcttctttg
    16861 ggttatattt cgttacgtac cgctttgaat cccgatgaag tgttggctgt tgcctatgaa
    16921 tatacttacg gaggacaggt ttatcaggta ggagaatttt ctaccgatgc tgtaactgct
    16981 ccgaatgctt tgattgttaa acttttgaaa agtactgcac agtctccttc acttgccact
    17041 tgggatttga tgatgaaaaa cgtctataat ttgggcgctc tgcagattca accagaaaat
    17101 tttcagttgg atattgttta tcgaaacgat tcggtgggaa ccgatctgaa atatattacc
    17161 gaaggaaaca tacaaaatca gcttttgctg cgtgtgatga atctcgatcg gcttaacaca
    17221 aaaaatgcag ctcagcccga cggaaaattt gattatgtgg aaggctacac tgtcctttct
    17281 tccagcggaa gaattatttt ccctgtgctc gaaccttttg gttcccattt gcggaaagcg
    17341 attggcaatg atcagattgc cgaaaaatat gtttttcagg aactttatga ttctacattg
    17401 gtagcggctc gtgaatacag cgaaaaaaac aaatttcgta ttgtgggaga atacaaggcc
    17461 agttcgggaa acgaaattcg gctgaatgcc atgaatatac cgcgaggctc cgttaccgta
    17521 acggcaggtg gagttactct tgttgaaaat gttgactata ctgttgatta cacgatggga
    17581 acggtcaaca ttatcaatac ttccattttg gaatcaggta gcaatattga tgtcaaattg
    17641 gaaaatcagt cgatgttcag catgcaacga aaatcgttgg taggcacaca tttggaatat
    17701 cagttcaatc gtgatttttc gttgggtgct accgtgatgc atctttccga aatgccttta
    17761 accactaaag tcaacacagg cgatgaaccc atttccaata ccatttgggg gctcaatact
    17821 tcgtggagaa aggaatctca atggctcacc aaccagctcg acaagctgcc ttttgttaat
    17881 gccaccaagc cttcaacatt ggctctgaac gccgaatttg cccagttgat tcccggacat
    17941 tcaaatgtta tcagttcggc ggggctggcc tatatcgacg attttgaatc gaccaaaacc
    18001 accatagacc ttcattatcc ggtattctgg catcttggca gcacgccttt tgaccccaat
    18061 ggtggacttt ttcctgaagc cgcactgagc aacaatgtgg attatggtaa aaatagagcc
    18121 ttactggcat ggtactatgt ggatccgata ttgaatgcca acagtcgtaa tactcccgaa
    18181 aatttaagaa acaatctgga atcacaatcc aatcatttaa cgcgcaatgt gttggaagaa
    18241 gaaatttttc cgaataaaga aaggcttgca acgcaaacat cccgaatgac cattatgaat
    18301 ctttcctttt atcctaacga aagaggtccg tacaatctag actgggaagg cataaatgaa
    18361 gatggttcgt tgaaaaatcc cgaaaaaaga tggggtggta tcatgcgtaa actggacgta
    18421 acggattttg aaaattccaa catagaatat atcgaatttt ggatgatgga tcctttcatt
    18481 accgataaag atggcacgca gaaaggtggt gatttgtata taaatttggg ggatatcagt
    18541 gaggatattt taaaagatgg gaaaaagttt tttgaacagg gcttgagtat cgatggcgat
    18601 gttaccaaaa gcgatactac cgtgtgggga agagttccga aaacgcaatc aacggtaaag
    18661 gcttttgaca atacaccggg agcgagaaaa tatcaggatg ttggtttaaa tggactatcc
    18721 accgatgaag aatttgaata tcctacttac aaaaaatatg tagaacaaat tacacgaaaa
    18781 atcagtcctc aaaccctcca aaaatggcaa gacgaccaat tttcgccatt gaatgaccct
    18841 gcaggcgaca actatcattt ttatcgtggt tccgattatg acgaacagca aaaagatatt
    18901 ctttcccgat acaagcatta taatgggact gaaggaaatt cacctgaagc agaaaatgtt
    18961 aacgaaagtt atacaacttc agctacttcg cttcccgaca tggaagattt gaatgaagac
    19021 aataccatga acgaatacga aaaatattat cagtatcggg ttcagattcg cagagatgct
    19081 tttcaggtag ggaaaaatta tatagccgac aaaattactt ccaatgtaaa gttggccaat
    19141 ggaaacgttg aacctgtaac ttggtatcag ttccgtattc ccatccgtga atacaacgat
    19201 aaaattggaa gcattcgcaa ttttaaatcc attcgtttta tcagaatgtt tcttaccgat
    19261 tttgaaaaag acattcattt gcgttttgct actttcgatt tggtaagggg tgaatggcga
    19321 agttacacca agtcattgta tccgaatgga ctgactcccc cttcggaagg gaaattggaa
    19381 gttcaggctg tgaatattga agaaaacgcc gatcgtaccc cagtaaatta tgtattgcct
    19441 cctggagtta ccagagaaac tgatccggga caaccacagc tattacagtt gaacgaacaa
    19501 tcgatggttt taagagtcag agatttggcg ccaaatgatg ccaaagctgt ttataaaact
    19561 acttcatatg atatgcgtca gtacaagcgt cttcaaatgt ttgtacatgc agaaaaattg
    19621 attgacgata tttccaattt gcaggataac gaattgacct gtttcatccg aatcggaacc
    19681 gatatggtga acaattacta tgaatacgaa attcccttgc gattgacgcc tcacggaaaa
    19741 tatctgggtg aaaatacaga gcagcgggag attgtatggt atcctgaaaa catgtttgat
    19801 tttgcatttt caaaactgat taatgtcaaa ctccaacgca acaagactct tcaggccagc
    19861 gcaggaaatg tttcgaatct tgtgccttat accatatttg atcccgataa gccaaaaaat
    19921 agaattacgg ttgtgggaaa tcctaccatt tccgatgttc agaatattat gattggcgtt
    19981 cgcaacagaa gtggcagcat aaaatcggga gaaatttggg taaacgaatt acgcatgtcg
    20041 gaatttgatg aatcgggtgg ttgggctgca atgggaaatt tggcagtcgg attatcggac
    20101 attgggacac tgaatctttc gggaagaatt gaaacttcgg gttatgggag tcttgaaagt
    20161 aatgttatga atagaaatct ggaagatttg cgtcagatga attttgcagc tgccatagaa
    20221 ttgggcagat ttttacctga acgggctaaa ttgcagattc ctacttattt ctcgtattcc
    20281 aacgaaaccg tatcgccaaa atataatccg ctcgatcagg atgtcgaact gtccgatgct
    20341 ttggataatc ttcccacaaa atcagatcgg gattccttgc tgaatttgtc gcaaacggtg
    20401 agcacttcaa aaagttttaa tgttgccaat gccaaattaa atataaaaag taaaaaacca
    20461 cagttttatg accctgcaaa tttgaagttt acctatgcct atattgaaaa caatgaacat
    20521 aattcggaga ttgagcaaaa tttgataaaa aaccagcgtg ctgcagtcga ttataatttc
    20581 aactttgcag cccagccgtg ggaaccgttt aaagatgtaa aagctttgaa cagtccggca
    20641 tttaaaatca tcaaggattt taatctgtat tatctgcctt cttctttaaa ttataacgca
    20701 aatcttaatc gatcgtattc tcaaatcaaa cttcggagtt ttgatgttta ttcgggcagc
    20761 tataccaacg atgaattgtc caatctcact ttcagcaaag attttatgtt caatcggcag
    20821 ttcgatatca aatatgattt gactcgttcg ttacatttca gttttcaaac agtaatgaac
    20881 gccaacatcg atgaacccta ttttacgccc gaaatgggga aggagtatta cgaagcatgg
    20941 cgcgatacag tgtggcacaa tatcaaaaaa cttggaacac cctatgttta tcagcaggta
    21001 tttactgctt cttggaatgt tccactcaat aagataccca tcacagactg gatgcaggca
    21061 aatgtttcct ataattccaa ttacaactgg aatcgaagtg cacaaatgga aggaggatcc
    21121 aatattggaa atacagctac cagcaccggt tcgtggcagt ttgatggaca actgaatttt
    21181 gaaacccttt acaataaatc caagtttctg aaaaacgttt tgaaccgaaa taaagttact
    21241 agtgccagac ctcagccttt taaaccggga aattatactc agaccgttca actcaagtcc
    21301 gaccagccgc tcaccattca gcatcgcttg ggcagtgagc gcatcaggct gacagcaaaa
    21361 gataaggatg gaaattctgt gttactctcc tataaagtaa aaaatgcttc ttcggtagag
    21421 attttaccca aacaaaatat ggacagcatc acaatcaaca ttgtcagtct ggatcccaat
    21481 cagcaatcgg tagtaaacaa aacgcgtgat attacattgg gtgctttaat gatggttcga
    21541 cggttttcta tcaattatag agaaaacaac agcacggtat tgtcgggatt tatgcctgaa
    21601 actggattta tggggcagca gaattacaat ggtatcgatg ctcccggata tgcgtttgct
    21661 tttggatttc aggaaaaaga ttttataaaa aaagctttcg aaagaggttg gctgaatgca
    21721 ggtgattctg tggttaaccc ggccacaact gcttttactt ccgatcttga tatcaaagca
    21781 acgctggaac ctattccggg ctttaaaatt gatttgaatg ggcggcgata cacggcttca
    21841 aatacttcgg tgttctacat gtacgacggt atgccgacta cttacagcgg aagttttaat
    21901 attacgcaaa ttggcttgtc cactgccttt aaaaagctgg gaaacgctac cgacaattac
    21961 aattcggaaa cttttaatgc tttccttgcc aatcggcaaa aagttgccga ccgtctgaat
    22021 cagaaatatg taggcacgcg atatcctcaa agtggattta tcaaatcttc caatttggca
    22081 ggcaaagaat atgacccatc attgggtgca tatcagctga attctgccga tgtgctgatt
    22141 ccagcttttc tggctgctta taccggacgt gatgctacaa aaatttctac caatccgttc
    22201 ctttctattt cgagtatttt gcccaactgg cgaatcagct atgatggact ttcaaaaata
    22261 gaatggttta aaaatcattt cagaaatgtg aatctgaccc atgcctattc ttttcgttac
    22321 agcattggtt cctattcttc ttattctaca tgggtgccta tggaggatga aagttcgttg
    22381 ggttatgttc gcgatgttca aaccgacaat ccgattcctt cgggtgctta cgattttact
    22441 agtgtttcgc tcaacgaaca gtttagtcca cttctgggta tcaatgctac catgaacaac
    22501 agtttgtcgg caaaaatcga ataccgcaag caaagaaacc ttgctttgaa tctttccagc
    22561 actcagctga tagaaagtgc cagcgacgaa tatgttttgg gatttggtta tctgttgaag
    22621 gattttgatg tgattttgcg attgaaatcg gacaagcagt cgaaaatcaa aaacgattta
    22681 aaactttcgg ccgatatttc ctataaagat attaaaacct tgttgcgcaa agtggaggag
    22741 aatattacgc aggcttcgag tggaaataag atgataacca taaaattcct ggccgattac
    22801 gtattcagtt ctaaagtcaa tattcaactt tattatgacc gtcagtcgag taccccactt
    22861 gtttcctctt catttccggt ttcatcttcc aattttgggg ctagtctcag gtttatgctg
    22921 accagataaa agtgattaca tgttgtttgt tgaattgtta aattttttga atatcaaacc
    22981 tcaattaaac tttggttgat tattattgtt ggaaggttaa tagaaatttt aatcacgtga
    23041 aatgaagaaa agattttttc tggtactgtg ttgctttttc gggttcttgt tttccgaagc
    23101 ccaaattttt gaaccgatac attgggatat cagtcataaa ctgaccggaa aaactacggc
    23161 cgacattatt ttcaaagctt ccatagatga agggtggcat ttatacgggt tatctttacc
    23221 tccaaatgga cctaaaccta ccgctatagt ttttgaaaaa attgaaaatg cccgcaaggt
    23281 tggtgaattg caagccgttt cgaacttgga gaaaaagttc gatccaaact ttggcatgga
    23341 gctgaactgg tttacgaaag aggctgtttt cattcagaaa attacattct cttccaagca
    23401 aaatgttcgt ttggaagggt atgtagaata catggcctgt aacgacaaaa gttgtttgcc
    23461 tcccacttcc gaaccgtttt cgattcgatt gagtaccact gaatttaatt ccatcaactc
    23521 cggggagcaa gcaccttctt cggaatccga aaaaattcag ccaacggaag atttcaaaaa
    23581 tgccaaacaa gtagatttaa gttccgaaga tactgccgta acttcttctg aatggtggac
    23641 accggtagtg aaagagttga acagctttgg gaatgtggtt tatcataagg aaacgacttc
    23701 gctttggtac attttgctgg ccggtttatt gggcggattt ctggctttgc ttactccatg
    23761 tgtttggcca attattccca tgacggtaag tttcttcctg aaacgttctg ccgttccttc
    23821 acgcggaaga aaagatgcag ttttatatgg tttgtccatt atagtcattt atgtgacatt
    23881 gggattgttg ataacgctta ttttcggcgc cagtgcattg aacagcatgt ccaccaatgc
    23941 aatttttaat ttgtttttct ttgttttgct tgtcgttttt gccttgtcgt tttttggtgc
    24001 tttcgagctg actttgcctt catcgtggtc aacaaaagtg gatgccaagt ccgaatcaac
    24061 ggtaggtttg ctgagtattt tgttaatggc ttttactttg gtgttggttt ccttttcctg
    24121 caccggtcct attataggga ctttgctggt tgaagttgca tccaccggtt ctttgcttgc
    24181 tccggctgta gggatgtttg gatttgcttt tgcgttggct gttcctttca ctttgtttgc
    24241 attcttccct tcgtggctcg aatcgttgcc acgctcaggc ggttggctaa acagggtaaa
    24301 agtagtgctg gctttcatcg aacttgcttt tgcactgaaa tttctttcgg ttgccgattt
    24361 ggcttatggc tggcatattt tggacagaga agtatttgtt tcgctgtgga ttataatttt
    24421 tgctttgctg ggtatttatt tgctcgggaa aattcgtttt ccacacgata gcgagtcgaa
    24481 acatacttct gttggtggac ttatgctggg aatcatttct ttggcttttg ccgtttacat
    24541 ggttcccgga ctttggggag caccgctgaa atcgatcagc gctttcgctc cgccacttta
    24601 tacgcaggat tttaatttgt acaataatga agttcatcca gcttttaccg attttgagga
    24661 aggaatggct tatgctgcaa tgaaaaaact gccggttatt attgactttt cgggttatgg
    24721 ttgtgtaaat tgccggaaaa tggaagcttc ggtttggact gatccacagg tgaaagcatt
    24781 gctcgacaaa aattttgtgc tgatttcgct gttcgtcgat gataaaacgc cattggaaaa
    24841 accttttgaa attaacgaaa atggtaaaac aagaaaaatt aaaaccgttg gtgataagtg
    24901 gagctatctg caacgatata aatttggagc caatgcacaa cctttttatg ttatgataga
    24961 ctatcacgga aaacctctga ataaacccta tgtttttgac accaatcctt ctcattttgt
    25021 caattggttg aatcgcaaat agtacatttt gtcttaatca gccctatgtg tttttatttt
    25081 tctgttattt tgtcatttaa ttcattgttt ggtagaaaca ttatattcaa aatagttgat
    25141 tggcaaactt attgtttaat atgagaatga gaaatttttt gagaaactta tcagagaatt
    25201 gtcttgtaat tctgatttag aaaaaataag ttggaatgga ttttttgtgt tatatagaaa
    25261 ttgattagct gaacttaaga gtttaccaat taaaaaaggg aggaaaaaat ggaaaaggaa
    25321 aataaagcgt cagaaaaaaa tcaatctgcc gaaaacgaaa aaaaacatca gcaacaacat
    25381 aaggaaaatg aagacaacat aattccacaa tcggaaaata gtgaagcaga aaatactgaa
    25441 aaagagttgg agcgattgag ggaagaatgc gaagatttgg cagacaagaa tttgcgtttg
    25501 ttggccgagt ttgacaatta tcgcaaacgt atgctcaagg aaaaagccga tttgctgaaa
    25561 aatggcggag aaaatgtgtt ggtgaatatt ttgccgcttg tcgatgactt tgaacgggct
    25621 ttgaaagcta ttgaaaccag cgatgacatt gaagctgtga aagaaggaat tcatttgatt
    25681 tacggtaagt ttgtcgattt tttgaacaaa aataatgtga aggaaattcc tactgaaaat
    25741 gtagaattta acactcagta tcacgaagca gtatccaaaa ttccggcacc ttcggaagaa
    25801 ttaaaaggga aagtaattga gtgtttatcg aaaggctaca cgttgaacga caaggtaatt
    25861 cgatatgcaa aagtcgtagt aggcgaataa ttttattcgc cttttaattt tttgaatttt
    25921 ttttgtttac aaatatgttg aggatgaggg agaattaagt tttatggcaa aaagagatta
    25981 ttacgaaatc ttaggtgttt cacgaacagc cacagaagta gaaattaaga aagcataccg
    26041 tcagaaagcc attcagtttc atccggataa gaatccgggc gacaagaagg ctgaagaaaa
    26101 cttcaaggaa gctgctgaag cttacgaagt tttaagtgat cctgaaaaac gtgaacgcta
    26161 cgatcgctat ggacatgcgg gagtaggaag ttcagcttcg agtggaagtt atggtggcgg
    26221 catgactatg gaagatattt tttcccactt tggcgatatt tttggcggtc attttggcgg
    26281 cttttcgggt tttggaggtg gatcgagaag cagcgctccc cgtatgcgaa gaggaaccga
    26341 tttgcgggta aaagtcaaat taacattggc tgaaatagct gaaggagtcg agaaaaaaat
    26401 aaaagtcaag aaatatgttc cctgttctca ctgtcaagga accggagcag ccgaaggttc
    26461 ggcgcccgta acctgttcga tgtgtcgtgg aaccggaagg gttacgcgcg ttcagcaaac
    26521 tattctcgga caaatgcaga ctacttccga ttgtccaacc tgtcatgggg aaggaaaagt
    26581 gattaaggaa aaatgtattc actgtaatgg agaaggaatt attcacgacg aagaaatagt
    26641 taccatcaac attcctgcgg gtgtggctga aggaatgcag ctaactttga gcgggaaggg
    26701 aaatgcggct cgcagaggtg gaatcaatgg cgatttgata gtggtgatag aggaagagcc
    26761 tcattcggaa ctgattcgcg atggaaatga tttgatttac aatttgttgc tgagtgttcc
    26821 gcaagcagca ttgggtggag ctgttgaagt ccccaccgtc aatggcaaag tgaaagttaa
    26881 catcgatgcc ggcacacaac cgggtaaagt actgagatta aggggcaaag gattacccga
    26941 tgtcaacaga tatggtaatg gcgatttgct tattaatatt ggcgtttata tccccgaaaa
    27001 attgagtaag gaggagaaaa ccattatgga aaaactggaa cagtcgagta attttaaacc
    27061 cagttcctca gctcaaaagg gttttttcag tcgttttaaa gatatgttcg attgaattct
    27121 aaatttgtat ttcatttcga aaaacagggg aaaaggagag aaaaagtgat ttctttcctt
    27181 ttttggtaag aaaataattt gccgattctg ccatagagta ttaaccgaat cttaaatttc
    27241 ttaaatatca acaagtatat ccaattaaaa acaaattaga ttgtaaaatt tttattttat
    27301 aaatgcaatt atctacttca acaataagct ttaataaact ttttacatga taattttcgc
    27361 tgttctgtcg cctgccacaa taatatagag ttgtcccggt attctgtttt ttaaagcatc
    27421 aatggtaaat ttgttgtaac gaggaatttc cgatacaatc aatttccctg taacatcata
    27481 aatccttact accgtactgg tattcggaac cgtaatggtt accgttttat cttctctgtt
    27541 gacgtaagct ttcaattgtt tgcctacatc gggcaaagta tgcacataaa tcaaatttga
    27601 aaaatcagtg atattttcat aaaggattac tgaatttctc aatgttttgt cgcttgcctt
    27661 cactttatag tagcaatcgg aattggaagg taaattaata accgtatccg ttgttgaggt
    27721 aatccattta tccttttcgt tataatttat tttttttgca aaccccaccg taatatcgtc
    27781 gatgatcaaa tatccgcttt ccttttgata agataatcga aattgagtat aatggtcaga
    27841 ttcggtaaag gtataagttt taatagcact gagactggta gttactgaaa cattgtcaat
    27901 ggttatccat ttgtctgagt cattttttgc ttcaacctta agtgctccat tcccagaaaa
    27961 agatttgacg aagaaagata attttatggc cggcaataca taattttccg tctgaatcat
    28021 ttcacctgag tttttaaatt gaattgccgg aacttctttt ccactgtacg aagcggacgt
    28081 actcaaagct tgagcggtaa ttgtccatcc cgatgatgga gtcaaaccat tatcaaaacc
    28141 ttcagtcgtt tctgatgatt cattggaaat ataataggca gtgaaataat atcccgtggc
    28201 atctgttact ggttcccaat gagctacgta actgctgatg cttacatcgg tagcttcagt
    28261 ggcaacagga ggaacaacat aaacaggtct tgtggatttt ccctgtaaat tcaaatagat
    28321 tggagtggca tctttagaag ttatagaaat aatttcattg tgaacatcaa gataagacgg
    28381 ttcggttggg ttatagcgta ccaaaattcg ggtagtatcc actacggaat caataggagc
    28441 aaggattaag tttttagtcc attctgcatt aaaatcattt tccaattgca tttcgaaatg
    28501 tttattttct gaaaaagcaa ggagaatatt ttcttttagt ttttttccgg aaatttttaa
    28561 aagttgtgca tttgatggcg ttccctgaac ggtttcaaaa gctaaaaaat tactcatagc
    28621 cccgacaatc ggagaatttt cttcccccat aaaatctaaa tttatataat tgttttcagt
    28681 aatatttgtg agagattttt ttagtttcgt accatttctt aaaaccaacg aacaggttgt
    28741 aatgttattt attcctggaa acggatctcc ggcgagtgtt tgttcagaac caataccgtc
    28801 ggcttcaaca attttataac ccagctttcc ggcaatatta ttgggtgtat tgttttccca
    28861 atcgctttga ttgaaattga tatgggtaac aagcaagccg tgtccgggta aataagcgtc
    28921 ccaccctgac ttttgcctgt tttccaacat aaaaaactct gaaggattgg gtgaatttcc
    28981 aaccaaatta tgattaccat cttttgaaat aagataggcg ctgttggatg aggttagagg
    29041 ttgcaatgtg aaatttcccg aacttttcag ttcgatgggt gttaaccaat tcaggaaaaa
    29101 tcgatcgaaa gccgaataag tgggaggcgt tttcccatca ttcaaataag ctccggcatc
    29161 catgatattc cagtaagata aggtaaaatg tgttcctcca tcggtaggat aataatcagc
    29221 cagaccaagc acatgaccaa attcatgaca gaaagtaccg atgccgcaca tgtttgcgcc
    29281 tctgcttgaa cgcaattcag aagaacaggc ataatcgaaa acgacaacac catcgaattt
    29341 tgtattgtaa ttggccaaag tccagcgatg tggccatatg gtattggccg gtgctccttc
    29401 tgcttcattg tagccggcat aatagacaaa aatattatcg acatatccat ccctgtcggt
    29461 atcgtattgg ctaaaatcta caccatcatt gtcggccaat ctacatgcgt caataatcat
    29521 ttgttgggga tttttatcca tgccatctga gccattttca ccgtaatagc ccattgtttg
    29581 tggcaaagta tagggcccta ccacatcaaa ttgggggtta aacttttcgg ttgaagtgtc
    29641 gtaaaaataa tcacgcgccg agccggttga accattttcg gaatatccat tttgattcaa
    29701 aagtttggta aaagcttctt ttggtgaact gacagtaaat gaattgtctg aaaaatttac
    29761 taaaattacc aatgatttcg gacttccggt caaaggataa gctttacgac cggatttttc
    29821 ggtaacagta acagatgaag ctcgttgtat gcggttcaat tgattttgtt tcgaaaaatc
    29881 tatccctttg ggaagttgtt ttataaaatt aatttcttcc ttcgtacgtt tttcgatatt
    29941 gtttacgcga tatttagacg gagctatttc ctttttttta cttaattttc cgtatttgaa
    30001 aaatccgtcc ttgtccctgg taatcaaata gccatctaaa gtagtctgat aatgaaaaaa
    30061 ttcgtctcca ttcagtttaa cagaaatttc tgtaccgtca ggctgaacca cctttaccgg
    30121 gaaaggatag gcagggactg cttttgtaaa ttgtggaaaa acaaaactaa aaacaaata
//
LOCUS       DAOTAX010000021        29982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1272_length_29982_cov_30.324857, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000021 DAOTAX010000000
VERSION     DAOTAX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29982
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_29982_cov_30.324857"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1369
                     /gene="coaBC"
                     /locus_tag="PLM04_03805"
     CDS             170..1369
                     /gene="coaBC"
                     /locus_tag="PLM04_03805"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HON02236.1"
                     /translation="MLQDKKIILGITGGIAAYKSATIVRLLVKQGAEVKVVMSASAKE
                     FITPLTLAALSKNPVLTDFFDPTNGQWNSHVDLGLWADAYLIAPATANSLAKMAAGIA
                     DNLLLTTYLSAKCPVFLAPAMDLDMFAHPATQRNLQILRSYGNLVIEPSFGELASGLE
                     GKGRMEEPENIVHFLDDYFTPKQLAGKKILITAGPTYEKIDPVRFIGNFSSGKMGFAL
                     AESCAAHGAEVWMIAGPVQLSLHHKNIQLIKVSSAQEMFDAVTEKFPEMDGAILSAAV
                     ADFTPMATAEDKIKRKGDRLTIELKPTPDIAATLGKIKTEKQFLVGFALETSNEIENA
                     QLKLKNKNLDFIVLNSLRDEKAGFGYDTNKITILHNTGKILSFDLKPKKEVAEDIVNE
                     LVKILNR"
     gene            1366..2268
                     /locus_tag="PLM04_03810"
     CDS             1366..2268
                     /locus_tag="PLM04_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HON02237.1"
                     /translation="MKKTFAVLIFLSFCFSFYAYSQELNCNISINSDQVQGTNKSVFN
                     TLQKSVSEFVNNRKWTEMTFLTNERIECSMNIIVKSVDGDNFTAEIQVQSRRPVYNSN
                     YNTTLFNFKDNDFTFSYKEFEPLEWNEASISSNLTAVLAYYAYIIIGYDMDSFSRLGG
                     TPFFQAAENIVNSAQGSNLPGWRAFESSRNRYALANNLTDEAFKNFRNYFYEYHRLGL
                     DEMANNPVNARARIAQGISVLREANRARPSAVIISTFLDAKTDELINIFSKGTDKEKA
                     LVVEILSDVNPTQTQKYEQILNKK"
     gene            2295..3953
                     /gene="recN"
                     /locus_tag="PLM04_03815"
     CDS             2295..3953
                     /gene="recN"
                     /locus_tag="PLM04_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HON02238.1"
                     /translation="MLKTLHISNYALISEINIGFNSGFSVITGETGAGKSIIIGALSL
                     ILGQRAESRFIKEGEEKAIVEAEFDIRDYRLEHFFEINELDFHPEQCIIRREITASGK
                     SRAFINDTPVSLQLLRELSAKLIDIHSQHENLLLSDDQYQLQVVDNMAQNASILKQYQ
                     QYYSQWKNLQTQLSQLQKTADKQLSELDYLKFQFQQLEEAHLSENEQDDLEMELETLS
                     HTEEIKQELQKAVFLLTDEQKSLVLLKEVIDAVGRIKSYISEGENWHKRLESVYIELK
                     DIAQEITSLEENIDFQPDRLEWVEQRLNEIYTLEKKFKLSSISELLSLKENLKEELQH
                     IESFDEEIDALKKKIAEAHKNVHQQAELLTKSRLNAVEPIEKFLKEQLSRLGMPNIQF
                     KVEITDAAEFSETGKDTVQFLFSANKNQSLQPVAQIASGGEVSRLMLSIKSLMAHRAG
                     LPTIIFDEIDTGVSGEIAHRMAEIMKEMSKNMQVIAITHLPQIAAKGNQHYRVFKDDS
                     GKTTQTHINLLDREERIWEVAQMLSGENVTEVALGNAKELLKSE"
     gene            4017..4892
                     /locus_tag="PLM04_03820"
     CDS             4017..4892
                     /locus_tag="PLM04_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HON02239.1"
                     /translation="MIQSMTGFGKATCEYEGKKIVVEIKSLNSKQLDLSLKIAAAYRN
                     KELEIRTEILQKLERGKIDVAIYIDNSGKDVANQINPSVFLSYMQQIKSLSQNLELPE
                     PTNWFDILLRLPDVMKTDNIELEENEWQALKKSIDKAIEQVISFRIQEGKSLENVFIS
                     KITNIGDLLEKISPYEAERIEKIRSRLKDNLQALSDKVDYDQNRLEQELIYYIEKLDI
                     NEEKVRLRNHLNYFLETMKLEPSAGKKLGFIAQEIGREINTLGAKANHSEIQKIVVKM
                     KDELEQMKEQVLNVL"
     gene            4957..5523
                     /gene="gmk"
                     /locus_tag="PLM04_03825"
     CDS             4957..5523
                     /gene="gmk"
                     /locus_tag="PLM04_03825"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HON02240.1"
                     /translation="MSGKLIILSAPSGTGKSTLVQYLLTRPFDLEFSVSATSRPPRGS
                     EQNGKDYYFLSKEEFQKKIAQNEFLEYEEVYPGCYYGTLRSEIDRIAAKGKNIIFDVD
                     VLGGLNIKKQYNGRALAIFIAPPSLEELKNRLTIRGTDPPEMIAKRLEKAEFEMSFAP
                     KFDVVIVNDDLEKAKKATEEVVRKFLEK"
     gene            5537..6115
                     /gene="nadD"
                     /locus_tag="PLM04_03830"
     CDS             5537..6115
                     /gene="nadD"
                     /locus_tag="PLM04_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HON02241.1"
                     /translation="MKKVGLYFGSFNPFHLGHQKLAEFLLDHQVVDEVWFVVSPRNPL
                     KKENDLLDENLRLEMVKIAIQDNPKLKVSDLEFSMPKPAYTIDSLHAFSSKYPENDFY
                     LLIGSDNAKVFDQWKNFKQILDEYPVLVYPRPGFDKEEIAKIYPQMKWLEGPQYNISS
                     TEIRQNIKQGKEVKDLLQPAVYQYIFKKKLYV"
     gene            complement(6118..8004)
                     /gene="ftsH"
                     /locus_tag="PLM04_03835"
     CDS             complement(6118..8004)
                     /gene="ftsH"
                     /locus_tag="PLM04_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HON02242.1"
                     /translation="MTKRAKYSWIYWLVIMFLFILMDWLIFPTTQGNRELSYNEFVDS
                     VRNGKVERIIFLQDKIVGEFKSDTNHISSMTQAPTAPWRIRIPDLEEQVQKQFVVSRI
                     PNMNEEYLINDLLAAKVNFKGHFQDNTVLNFFLNWILPFVLFMLLWGYFFRKMGNKNP
                     MLEIGRNKAQIQAEKPANPITFKDVAGIDEAVEEVKELVDFLKNPSKYTRLGGKLPKG
                     VLLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFNQAKAHAP
                     CIIFIDEIDAIGKSRAHSGYYAGGYDERENTLNQLLVEMDGFDASSGVIIMGATNRPD
                     VLDPALLRPGRFDRQVLVDKPDMNGREAIFKVHTKNIKLASDVDLKRLAAQTPGFAGA
                     EIANVCNEAAILAVRNNRDEVTMMDFEAAIERVIAGLEKKNKLINEKERKIVAYHESG
                     HAIVGYFTPGADEVQKVSIVPRGIGALGYTMQMPLEDRYLLSESELIGKIKGLLGGRA
                     AEELIFGEVSTGASNDLERATQLARNMITVYGMSKKLPNISLVNQSTTGFLGGGPGIE
                     RRSEYVEKMIDEEMTEIIRSCYENAKKLLSEKKDLLEKMAITLLEKEVLSYNEIKEIL
                     GEKN"
     gene            complement(8185..>9971)
                     /locus_tag="PLM04_03840"
     CDS             complement(8185..>9971)
                     /locus_tag="PLM04_03840"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HON02243.1"
                     /translation="SIWFYVLQFVIGALDNATNLTIASDSSMKLIIITTTTLTFGMGA
                     STQALFARVGGGIFTKAADVGADLVGKVEENIPEDDPRNPATIADNVGDNVGDVAGMG
                     ADLYESYCGSILSTAALGAVAFAAKGTAVQFNGVIAPMIIAAVGIILSIIGIFMVKSK
                     EDASQKQLLSSLSRGINFSAIFIAIFSFLILKYLNIPNYLGIWGSMLAGLFGGVAIGK
                     ITEYFTSAGFKPTQRISESSKTGPATVIIAGIGTGMISTAFPVLVVGISIILSYLFAS
                     GFDFSNISMGLYGIAISAVGMLSTLGITLASDAYGPIADNAGGNAEMSGLAPEVRRRT
                     DALDALGNTTAATGKGFAIGSAALTALALMASYLEEIKIAIARIPEKTELFFNAMQTD
                     IHQASIPQLIEYFQINLMNPRVLVGAFIGAMSAFLFCGLTMNAVGRAAQKMVQEVRRQ
                     FREIAGIMEGKAEPDYARCVAISTKGAQKEMLLPSLLAIIIPILTGVIFGVSGVMGLL
                     LGSVSTGFILAIFMANSGGAWDNAKKYIEEGNLGGKGSENHKAAVVGDTVGDPFKDTS
                     GPSLNILIKLMSMVSIVMAGLTVAWSLL"
     assembly_gap    9972..10071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10072..10535)
                     /locus_tag="PLM04_03845"
     CDS             complement(<10072..10535)
                     /locus_tag="PLM04_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HON02244.1"
                     /translation="MENKVFFLVPVSAVLALFFAWYFFHQMKKESEGTISMQKIAKYV
                     REGAMAYLKQQYKVVGIVFLIITIIFSFMAYYLKIQNPWVPFAFLTGGFFSGLAGFIG
                     MRTATYASARTANAAQQSLNAGLRLAFRSGAVMGLVVVGLGLLDISIWFYVL"
     gene            complement(10609..11850)
                     /locus_tag="PLM04_03850"
     CDS             complement(10609..11850)
                     /locus_tag="PLM04_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HON02245.1"
                     /translation="MSLVEILFYLILFFIVGDFILERYLSFLNFQYSKNELPDNLKDI
                     YDADKYGKQQEYFRSNIRFGTISSTINFIIILIFFCLGGFGWLDTLLRRITEHPILLP
                     LLYFGVLFVAETLISIPFQWYDTFVIEQKFGFNKVTPKLFVQDHLKEFALSVFLGGIL
                     LGAIVWIYQLTPKYFWLIAWSVVTLFSLFMSMFYSQIIVPIFNKQKPLEEGELRDEIE
                     KFATKAGFKLDNIYIIDSSKRSTKANAYFTGLGNKKRVVLYDTLLDKLTSEEIVAVLA
                     HEIGHYKKKHTLYQFLISLPESLLLFFLLGLILKSDVFAQVLGGTTASFHLNVIAFGL
                     LYTPVSLLLSICTNILSRKFEYQADAFAANFGWAKQLQSGLKKLAATSLSNLMPHPLY
                     AFFHYSHPTLYQRLTKLSDAQ"
     gene            12008..12967
                     /locus_tag="PLM04_03855"
     CDS             12008..12967
                     /locus_tag="PLM04_03855"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON02246.1"
                     /translation="MKRLQIFLFILITLFSLSCSNKTASDKISIAIPEGPSVVSFIKM
                     MDDSVSIEGKKVEYIVKSDPQQIQALMMQNKVDFAILPTNMAANLFNKGIKYKMIACP
                     VWGTLYILSNLPEVKTLNDLQNRKVSIFGQGTTADILFQKLIQKKGIEKVFIDYRFAG
                     NFELSQALRLKKTNIAVISEPLVSKLLEQDSTIHIVEKLSCEEYLNEKDRDIFVQTAF
                     LVNEKLERNNPQIIKEICNAYRASCNYVTQHPEEAAVLLVKHGILPETNVAKKSILLC
                     SINYVAAFAIEDEIYSYLKIFYDFNPESIGGKLPSENFIFQVF"
     gene            12979..14379
                     /locus_tag="PLM04_03860"
     CDS             12979..14379
                     /locus_tag="PLM04_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON02247.1"
                     /translation="MKNQNKKIFPFILSVFSLAIIWQIISISIGYPAIFPSIFDLISD
                     TLHLFTRSEFYFALLGTVSRGLLGFVITFFIALFFGSLSVFNSFFKDFFHPIVVFTRS
                     IPVISIVLVALLWFSPPFLPVFVAFFSMFPILFQSIQSGLENVDKKWVEMGKVFGLSS
                     RKRFLHIYYPAARSTIFDGISSAMGFGWRAVIIGEALAQPVHGIGSQMKAAQAYINVS
                     EIIAWTLAAILISYIFEKIILFLKKCNRPIQLPKSEECKLHKFNQIVDKKEITIYNLK
                     KKFNDYTVFSSFNENFKDTEINFIKGPSGIGKTTLLRLIARLEKPDEGTIYYSSKFSF
                     GYSFQDVRLLPWLTVKENIAFPLLYKKTDRQELTDNIDWLLNNMELTEHQNKFPDQLS
                     GGQLQRVGLARALVAQPDVLLLDEPFTGFDDDLKTRIQDFLSKWFEDFQPLVIWATHE
                     NILFPEKNRREIQLKN"
     gene            complement(14487..15008)
                     /gene="rimM"
                     /locus_tag="PLM04_03865"
     CDS             complement(14487..15008)
                     /gene="rimM"
                     /locus_tag="PLM04_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HON02248.1"
                     /translation="MISFTEVIPIGQIIKLHGINGEMAFMSNYEGLDFDNIPFLIFDM
                     HGILVPFYLKEWRFNSENSGFLKIEDVNNEKKAKNFLGLTIYLHKKFLNILESDVDMD
                     NNTFIEFRIFDKDNNFIGIVTDIDTSTQNVLFIVDNGEKQILIPACQDYILQIDEMKK
                     IIYMDLPEGLLEL"
     gene            complement(15077..16777)
                     /locus_tag="PLM04_03870"
     CDS             complement(15077..16777)
                     /locus_tag="PLM04_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02249.1"
                     /translation="MAQDSVFTRNITVEREYQPILKNTGKLNVTPKLIEPEAKPLPAQ
                     YTDLFIPLSINNNIHPLSSTEFFFNNRVEQNNFLQLGLGNFYNSSANFLYSLLNRPNT
                     KLDFYMNHIGTFGDKLHSTTKSGLVFDQQLKSLHLFAGVETQHERLKFYGFNFNDSAS
                     VQLNELAKNHGSSTYIERQFTSITRAPHSFTLQELSETPGTETFWHLNAFGGLQSLPL
                     TSDINYSAKLNYHFFQNKFGIKENRIHLTGNVIMPFHENNIGLDVQLHQIKYTANEAP
                     LFNFWDYYSVIMLNPFYSIEQTNWKLRMGLKAAFSMKHGRSFSPAADIAAEWNVFPQW
                     MTLYGGLSGDYEINSMDRIFSENCFLFSDTRIKDTYTPFLSYGGVKIKPAKNLLVDFY
                     ADYRFTDQQYFYVNKEYITEELNAPDSVLFTNRFNVAYSDAYHFKIGGRAAYNDEKNR
                     FNMEFRTTYNWWDTKDTALPWLQPTWETDLRANLGITSNITTSLYFFYKTGRYAQLGN
                     RSVKMDNIMDVNLGISYIVSKSLSVYLHLNNLLNNKYQYFYGYDVQGFNLMTGVAFSF
                     "
     gene            complement(16925..19969)
                     /locus_tag="PLM04_03875"
     CDS             complement(16925..19969)
                     /locus_tag="PLM04_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02250.1"
                     /translation="MAKKNIPFLTLILFLCLSNLFLSAQHTLFFASEDKDFIQGKELY
                     FQQKYAASYRSFEQFLEKKTFPSDGQFQEAEYFLSANAYELRKENALSLLQTFLTKHP
                     YTPFSEEIYFMIGVLNCEQKNYTVARENFSQVNEQELSENKIPEFLFYKGYTAIQTQH
                     YEEAGKIFSRLKLQNSPFRVDATYYYAYSEYMMGNYLKALPDFLAIENHPDYQISVPY
                     FLVQIYYVEKQYAKLKEKADFLLQKYPNNNNNAEIFRILGELAYGEQNYAQAISYFKK
                     YENLSPKVLRNDVYLLGLSYFQLKDYSNAIQYLSKVTTEPDEISENAYLHLGTSYVFL
                     NDKTNARLSFEAALQTHFNPTVREEALFNYALTCYETTSAFGESVAAFTRFLSEFPNS
                     KYADEAYQYLTSVYLTTKNYELAYQSILKIKKLNTSLTETKQYLLYQMGVNAFAQKDY
                     GKAIEYFSLAHTSAPKGKYSAECLFWRAECYYRTNNPDQAIVDLETFIKNPLSRNSEN
                     LIYAYYSLGYGYFDQKNYIKALEWFSKYLENENSEKSMLYADALTRAGDCHFYARNFK
                     NALSFYEKAAFTSPNTADYSIFQAAYVEGLQKNYLNKIYRLEQLLLKYPDSEYGDDAL
                     YEIGRSYLMLKNEKMALSAYERLMNQYPSSVLSRKAALESGMIYFNRQDFENAIVIFK
                     YVAEKYPDTEESYIALKSMESAYIELNDVNGFLQYTKNSGRIVRPLSVMQEDSISYIA
                     AEKQYINGRYTVAISGFENYLNNFCPGGRYCTIAQFYLADSYYRTQQRDKALKAYEVV
                     LKTNAAEYVEQAALHCAEITYDQKDYASAFNYFKQLKQVAQNSANKQAAHLGMLRCSY
                     FTNDYQSLVDIANVILQDPYSEAIVKTEARFYRAKAYLLHQQLNAAIEDLKIISADTK
                     TAFGAESKYLLASVYFQQEKLTEAENEILDFAKKNTPHQFWLARSFVLLADIYIKKGN
                     DFQAKQYLLSLKRNYTIQDEIQGLIDERLSAISQREKKNIIH"
     gene            complement(20043..20585)
                     /locus_tag="PLM04_03880"
     CDS             complement(20043..20585)
                     /locus_tag="PLM04_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON02251.1"
                     /translation="MDFFSKFSFCPSCGSADFEINNEKSKRCKACGFVYYLNASAAVA
                     AFILNEKKELLVCRRKKDPAKGTLDLPGGFVDNYETAEEALKREIMEELQLEVENPTY
                     LFSLPNEYEYSNLLIPTLDLFFLCQIKKDLIPIPSDDVEDCFYVPLEKIQLELFGLKS
                     VKKAVEIFLREQIGKKCQNI"
     assembly_gap    20698..20797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20889..22256
                     /locus_tag="PLM04_03885"
     CDS             20889..22256
                     /locus_tag="PLM04_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02252.1"
                     /translation="MGKKLLLPGDDEFKDLVHRYEQYLSGNANGYFDVEEFENIAEYY
                     LLRGRTKDSSRAIDFGMKLHPGSTALKAKRAKIYLAVDEVKKAYQLLSSFTESDYETL
                     LLKIEALVRMGRHAEADKLIKGLTTEKTADLDNIFLDIAYIYLENNKFEKASEFLLKG
                     FQINPKNIDLLFELAFSFEQMEEKEKAIEIYNRILDLDAFSGEAWFNIGQIYFNLQEF
                     PKALEAYDFVLAIDPNDSLAYLQKAHVHYQLEEFYTAIEYYAHYAAMTDDPASTSLYI
                     AECYEKLEQYDKAVDYYKYLLEQDPDNTDLLISIGSCLLEQEKFSASIEYLSRAIELN
                     DSLADAWVYLAEAYLAIDNLDIALKSYLLALQLDSEQPEVIMAVANIYLEKGDYETAL
                     NFYLAVEAMSNSLEIVNVFIAATYYKLGNKTEAYRYLKKGIEDNEDVLNLFLEICPEA
                     IETIH"
     gene            22358..23095
                     /locus_tag="PLM04_03890"
     CDS             22358..23095
                     /locus_tag="PLM04_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HON02253.1"
                     /translation="MRLYGLIGYPLKHSFSKRYFAEKFRREGIDARYDLYELQRIDDF
                     PELVKMPGLCGLNVTIPYKESVIPFLNELDEVAEKIGAVNVIKFLRKNNHLWLKGYNS
                     DIIGFEKAIIPFLKTFHDKALLLGTGGAAKAISFVLAAHNIKVTHVSRKPGINKITYN
                     DLNEELMREHKLIINATPLGTYPNLCSCPDIPYEHLTPQHLLFDVVYNPPKTLFLKKG
                     EEQGATIINGEAMLRGQAEAAWEIWNK"
     gene            complement(23243..>24047)
                     /locus_tag="PLM04_03895"
     CDS             complement(23243..>24047)
                     /locus_tag="PLM04_03895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HON02254.1"
                     /translation="IMFGIMLLLPVFGTAQNNNSNKKSDIQTKSISYKIGDQNFRGYL
                     AWKDDGKKTKPGILVVHEWWGFNEYIKMRTDMLAELGYVAMAVDMYGDGKVYDTAAEA
                     QNAAGYIFSHPELLKERMEKGYEILTSEKTVDKKHVSAIGYCFGGTVVLNAAAMGLPL
                     ETVVSFHGGLNGFKASPAMKNTKVLICNGAADMFVTQADIDNFKSEMQKYNITYEFKN
                     YEGATHAFTNKYSTEAGKKFNMPIAYNEAADKASWQDMILFFNKYFPNK"
     assembly_gap    24048..24147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24225..24932)
                     /locus_tag="PLM04_03900"
     CDS             complement(24225..24932)
                     /locus_tag="PLM04_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HON02255.1"
                     /translation="MIKVEIWSDVVCPFCYIGKHKFEKALEKFPYRENVQVEWKSFQL
                     DPFAVTNPELKTMEHLAAEKGWSMEQALQSTAFITQMAKEVGLEFHFEKAIVVNSFKA
                     HRLLHLAKKHGKQNEMEEKLFAAYFTDGKNIDDNEILLQLAKEVGLEATETNEVLLSD
                     AYSDEVKHDIAHAHQLYVRGVPYFLFDDKFAVSGAQDESVFLQALNRALAEHKKTDII
                     EFSGENGGVCGPDGCEI"
     gene            complement(24982..25944)
                     /locus_tag="PLM04_03905"
     CDS             complement(24982..25944)
                     /locus_tag="PLM04_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HON02256.1"
                     /translation="MTIIVTFKTFPEQKEYLKNVLGKEMQIYFKEDCTKEELENLMTK
                     ADVLLSWNPVQEGINKQNLPMDNIRFMQLLSAGYDHIRLEDFPEKLQIAANQGAYAEP
                     MAEHVMAMLLALSKRLYVYHNDLAKGNFSQIKSQTKFLKGSVAGIIGFGAIGKATARL
                     LRPFGVKLMAINSSGKTDEEVDFIGTLKDLDYVLQNADSLILSIALNEETEGLINKER
                     LELMKEDAVLVNVARGAIVEEAALYEHLKTHPNFYAGIDAWWVEPFKDRKFELHYPFF
                     ELPNLLGSPHNSAMVHNALLIGTEHAVQKILKFIHGEKVPGLIR"
     gene            complement(25956..26591)
                     /locus_tag="PLM04_03910"
     CDS             complement(25956..26591)
                     /locus_tag="PLM04_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HON02257.1"
                     /translation="MEQNKITIEFFHDVLCAWCYALSPRVEKLVEKYFDRIEVIHRSF
                     ALAPEPNGIEKIFGSKEEGKIQILGHWRAANMNDDAHRMHPELMATRTFDYPYSTRGL
                     LACKAAELQGGNEMHHKIFNRIQKAHLTECLNINDVEVLKMCAREVGLNVEQWEKDFH
                     SEKVKQMLDEDFYLAYQYGINSVPTLIANGKYKLTGAQPYETLEKWINQIK"
     gene            complement(26599..27417)
                     /locus_tag="PLM04_03915"
     CDS             complement(26599..27417)
                     /locus_tag="PLM04_03915"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HON02258.1"
                     /translation="MKISEKLPVFFIPHGGGPWHVMDDFMGDPQGYGMLRNYLKKIGN
                     DFHDKIKAILVVSGHWEESVPTVNFGEHPSLLYDYYGFPEFTYQLSWPAPGDPQLALK
                     IENLLLEAGFTTARENLRGFDHGVFVPLMVAFPQADIPVVQLSLIKGLRPAQHIAMGK
                     ALESLRSEGVLIIGSGMSFHNLRGMMSGNQAISKISEQFDKWLTHAVENKNPEIRNQL
                     LTQWLSAPGARESHPRSEHLVPLFVVAGAAGNDLGKQDFSGTLLKVKISSYRFG"
     gene            complement(27465..28025)
                     /locus_tag="PLM04_03920"
     CDS             complement(27465..28025)
                     /locus_tag="PLM04_03920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HON02259.1"
                     /translation="MIKIGIIVGSTRPGRNSEAVAKWVYEVASKRNDAIFEIVDIADY
                     NLPLLDEMIPASMHQYQKEHTKKWAEKINEMDAYVFVTPEYNHAPTAALKNAIDFVYA
                     EWNNKAAGFVSYGSAGGARAVEQLRQIMGEIQIADVRAQVTLSLFTDFENFSVFKPAS
                     SHEGSLHAMLDQLIAWGTALKTVREK"
     gene            complement(28033..28266)
                     /locus_tag="PLM04_03925"
     CDS             complement(28033..28266)
                     /locus_tag="PLM04_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02260.1"
                     /translation="METKKKLRCPLGIPGGIIAALIGLIGIIVNIVAFNWFQLIISLA
                     LLLLALPFVRVTMMVHMANDRLDELEEKINSKK"
     gene            complement(28282..28548)
                     /locus_tag="PLM04_03930"
     CDS             complement(28282..28548)
                     /locus_tag="PLM04_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HON02261.1"
                     /translation="MNKKTTIELFYTLTCPNCKLTKQMLKEVLPQFEDKFSLKTTLAN
                     SPSGMIRTMKLGIHTVPTLLIDNKVVFKSVPTKEELIEKLKNYQ"
     gene            29064..29837
                     /locus_tag="PLM04_03935"
     CDS             29064..29837
                     /locus_tag="PLM04_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HON02262.1"
                     /translation="MDLVGFFILGIAAGILSGLFGIGGGIVIVPSLIVIFGLDILDAN
                     AVSLAAMLLPVGILGVWAYYKEGYVNIGESLWVALGLFLGSFVGGGIAVNIGESLLAK
                     LYAGILIYIAISYFQLPKYFRKKQPIEKEEKPREKKSFWAFVLIGLAAGVFAGLFGKG
                     GGLVIVPMLIHFFHYDSRSAAATSLAALQLPVGLPSVLIYAENGHLNLLFAALIALGI
                     FVGVLIGSKIGIKIPPSIFKKIYAVFLLAIAVYMAFEYI"
     gene            complement(29883..>29982)
                     /locus_tag="PLM04_03940"
     CDS             complement(29883..>29982)
                     /locus_tag="PLM04_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HON02263.1"
                     /translation="YFGDAIFDRLLVAAVSYNTDFKSRIYNRNHCG"
BASE COUNT         9453 a         5463 c         5319 g         9447 t
ORIGIN      
        1 ccaattttca attcgatttt tctacctgat gatgctaatg gacaatttgg gattaaatga
       61 ataaaaagtg ggtaacacta tcagtattat gttttttgaa gtattacagg aaaattgaaa
      121 attatcttgt aaatttgcag gatataattc atccataata aaggttgata tgctgcagga
      181 taaaaaaatc attttgggca ttacgggtgg aattgctgct tataagtctg ccacgatagt
      241 tcgtttactg gtgaagcagg gagctgaagt aaaagtggtt atgtcggctt cggcaaaaga
      301 atttattaca cccttgacac ttgctgcact ttccaaaaat ccggtattga ccgatttttt
      361 cgatccaaca aatggtcaat ggaacagcca tgtggatttg ggcttgtggg ccgatgcata
      421 tttaattgca ccggcaacag ccaattcatt ggctaaaatg gcagccggca ttgctgataa
      481 tttgcttttg actacttatc tttcggccaa gtgtcctgtc tttttagctc ctgctatgga
      541 tttggatatg tttgctcatc ctgctacaca gcgcaacttg caaattcttc gttcgtatgg
      601 aaacctggtt atagaacctt catttggtga attggcaagt ggtctggaag ggaaggggag
      661 aatggaagaa cccgaaaata ttgttcattt tcttgacgat tattttacac ctaaacaact
      721 tgcggggaaa aaaattctga ttactgccgg tccaacgtat gaaaaaatag atccggttcg
      781 ttttattgga aatttctcat ccggaaaaat gggttttgcg ttggctgaat catgtgctgc
      841 tcatggagct gaagtttgga tgattgcagg gcctgttcaa ttaagtttgc accataaaaa
      901 tattcaactc attaaagttt catccgctca ggaaatgttc gacgctgtta cggaaaagtt
      961 tcccgaaatg gatggtgcca tattaagtgc tgcagtagca gattttacac cgatggctac
     1021 tgccgaagac aagataaaac gtaagggcga tcggttgacc atagaactaa aaccgactcc
     1081 ggatattgct gctacactcg gcaaaataaa aaccgaaaaa caatttttag taggttttgc
     1141 tctcgaaact tccaatgaaa ttgaaaatgc acaattgaag ttgaaaaata aaaatttgga
     1201 ttttattgtt cttaattctc ttcgtgacga aaaagccgga tttggctacg ataccaataa
     1261 aattaccatt ttgcataata ccggcaaaat tctttccttt gatttaaagc caaaaaaaga
     1321 agtggcggaa gatatagtta atgagctggt aaaaattttg aatcgatgaa aaaaacgttt
     1381 gctgttctga ttttcttatc attttgtttt tccttttatg cgtattctca ggaattaaac
     1441 tgcaatatta gcattaattc cgatcaggta caaggaacaa acaaatctgt tttcaacact
     1501 ttgcagaaat cggtttccga atttgtgaac aaccgcaagt ggacggaaat gactttttta
     1561 accaacgaac gaattgaatg ttcaatgaat atcattgtaa aatcggttga tggagataat
     1621 tttactgctg aaattcaggt acagtcgcgt cggccggttt acaattcgaa ttacaacaca
     1681 actcttttca atttcaaaga caacgatttt acgttctcat acaaggagtt tgagccgttg
     1741 gaatggaacg aggcatccat tagttccaat ctcactgcgg tattggctta ttatgcctat
     1801 atcattatcg gctacgacat ggattctttt tcaaggttgg gaggcacacc attttttcaa
     1861 gccgccgaaa atatagttaa ttctgctcag ggaagtaatt tacccggatg gcgagctttt
     1921 gaaagcagcc gaaatcgtta tgctttggca aataatttga ctgatgaagc tttcaaaaat
     1981 tttcggaatt atttctacga atatcatcgt ctgggattgg acgaaatggc caataatcca
     2041 gttaatgccc gtgcccgaat tgctcaagga atttccgtgt tgcgcgaagc caatcgtgcc
     2101 cgcccatcgg cagtaataat ttctacattt ttggatgcca aaacggatga attgatcaat
     2161 atattttcta aaggaactga caaggaaaaa gcattggtcg tagaaattct gtcggatgta
     2221 aaccccacac agacacaaaa atacgaacag attttaaata aaaaataaat actttttctg
     2281 actaccacat atttatgctt aagacgcttc atatcagcaa ttacgcattg atttccgaaa
     2341 ttaatatcgg ttttaattcc ggcttttcag ttattacagg cgaaaccggt gctggaaaat
     2401 ccatcataat cggtgcgctt tcgttaatac tcggacagcg ggctgaaagt cgttttatca
     2461 aggaaggaga agaaaaagct atagttgaag ccgaatttga tattcgggat tatcgtttgg
     2521 aacatttttt tgaaataaac gaattggatt ttcatcctga acagtgcatt atccgtcgtg
     2581 aaataacagc atcgggaaaa tcaagagctt ttatcaatga tacgcctgta agtttacagc
     2641 ttttacgtga actcagcgcc aaattgatcg atatacattc tcaacacgaa aatttattgc
     2701 tttcagacga ccagtatcaa ttgcaagtgg ttgacaacat ggcacaaaat gcttcgattc
     2761 tcaagcaata tcaacaatac tatagtcaat ggaaaaacct gcaaacacag ctttcccaat
     2821 tgcagaaaac tgccgataaa caattgtctg agctcgatta tttaaaattt cagtttcaac
     2881 agttggaaga agcccatttg tcggaaaacg agcaagatga ccttgaaatg gaactggaaa
     2941 cattgagcca cacggaagaa attaaacagg aattgcaaaa agccgttttt ttacttaccg
     3001 atgaacaaaa aagtcttgtt ttgcttaaag aagttatcga cgctgtagga cgaataaaaa
     3061 gttacatttc ggaaggagag aattggcata aacgtttaga gtcggtttat atcgaattga
     3121 aagatatagc tcaggaaata acaagtttag aggaaaatat tgattttcag cctgatagat
     3181 tggaatgggt cgagcaaaga ttgaatgaaa tttatacgtt ggaaaagaaa tttaaactga
     3241 gttctatttc cgaattatta tcgctgaaag aaaatttaaa agaagaattg cagcacatcg
     3301 aatcttttga tgaagaaatc gacgccttga agaaaaaaat tgccgaagct cataaaaatg
     3361 ttcatcaaca agccgaatta ttgacaaaaa gtcggttgaa tgccgttgag ccgatagaaa
     3421 aatttttgaa agagcagctt tcacgtttgg gaatgccgaa cattcagttc aaggttgaaa
     3481 ttacggatgc tgccgaattt tcggaaacag ggaaagacac ggttcaattt cttttttcgg
     3541 ccaataaaaa tcaatcgttg cagcctgttg ctcaaatcgc ttcgggaggt gaggtttcac
     3601 gtttaatgct ctccatcaaa tctttaatgg cacatcgagc cggtttgcct accattattt
     3661 ttgacgaaat cgatacgggc gtttccggcg agattgctca ccgaatggct gaaattatga
     3721 aagaaatgag taaaaacatg caggtaattg ctatcacaca tttacctcaa attgctgcca
     3781 aagggaacca acattatcgt gtttttaaag acgattcagg caaaactaca cagactcata
     3841 tcaacttgct cgatagggaa gaacgaattt gggaagttgc gcaaatgctc agcggtgaaa
     3901 atgttacgga agttgcattg ggcaacgcca aagaattgct taaaagcgaa taaaaaaccg
     3961 gaaatctgaa ttacggtaac ataaacaatt gcaaaaatca ctttttacaa aatattatga
     4021 tacaatccat gacaggtttc ggcaaagcta cctgtgaata tgaagggaaa aaaattgtcg
     4081 tagaaattaa atcactcaac agcaaacaac tggatctttc tttgaaaata gcggctgcat
     4141 accgaaataa agaactcgaa atcagaaccg aaattcttca aaaattggaa cgcggaaaaa
     4201 ttgatgtggc tatttacata gataactcag gcaaagatgt tgctaatcaa atcaatccat
     4261 ctgttttttt atcttacatg cagcaaatca aatctttatc acagaatctt gaattgcctg
     4321 agccaacaaa ttggtttgat attttgcttc gattgccgga tgtaatgaaa accgataaca
     4381 tcgaattgga agaaaatgaa tggcaagcac ttaagaagag tattgacaaa gcgatagaac
     4441 aggttatttc tttccgtatt caggagggaa aatctctgga aaacgttttt atctcaaaaa
     4501 ttacaaatat tggcgattta ttggaaaaaa tttcgcctta cgaagcggaa agaatagaaa
     4561 aaatacgttc cagactcaaa gacaatctgc aagcactttc tgataaggta gattatgatc
     4621 aaaatcgttt agagcaggaa ttgatttatt acattgagaa gctcgatatt aatgaagaaa
     4681 aagttcgttt gcgaaatcat ctgaattatt tcctcgaaac catgaaactg gaaccatcgg
     4741 cgggcaagaa actcggtttt attgctcagg aaattggtcg tgaaatcaat acgcttggag
     4801 ccaaagccaa tcacagtgaa attcaaaaaa ttgtggtgaa aatgaaagac gaactggaac
     4861 aaatgaagga acaagtattg aatgtccttt aatttgataa atttctttta ttataaggta
     4921 aaacaattgt actacgctta aaatttcact ttcttcatgt ccggaaaatt aattatttta
     4981 tccgcaccat caggcacagg caaaagcacg ctggttcaat accttctgac acgtcctttt
     5041 gatctggaat tttcagtgtc agctaccagt cgtccaccgc gcggaagcga acagaacggg
     5101 aaagattatt attttctttc taaagaagaa tttcagaaaa agatagcgca aaacgaattt
     5161 ttggaatacg aagaagttta tccgggatgt tattatggaa ctttgcgaag cgaaattgat
     5221 cgcattgcag cgaaggggaa aaatatcatt ttcgatgtgg atgtgcttgg aggtcttaat
     5281 attaaaaaac aatacaatgg cagggcattg gccatcttta ttgctccccc aagtttggaa
     5341 gaattaaaaa atcgtctgac gattcgaggt accgatcctc ccgaaatgat tgccaaacga
     5401 cttgaaaaag ctgaatttga aatgagtttc gctccaaaat ttgatgtagt aattgtgaat
     5461 gacgacttgg aaaaagctaa aaaagcaacc gaagaagttg ttcgcaaatt tttggaaaaa
     5521 taagttttcc tgcaaaatga aaaaagttgg attatatttt ggttctttca atccttttca
     5581 tctcggacac caaaaactgg ccgaatttct tcttgatcat caagtggttg atgaagtttg
     5641 gtttgttgtt tctcctcgca atccgttaaa aaaggaaaat gatttgctgg acgaaaatct
     5701 tcgcttggaa atggtaaaaa tagccataca ggacaatccg aaattaaaag tttccgacct
     5761 cgaattttca atgcctaaac ctgcatatac cattgacagt ctgcatgctt tttcttccaa
     5821 ataccctgaa aacgattttt acctgctcat tggtagcgat aacgcaaaag tctttgatca
     5881 gtggaaaaac tttaagcaaa ttctcgatga atatcccgtg ttggtttatc cgcgtcccgg
     5941 attcgataaa gaagaaattg ccaaaatcta tcctcaaatg aaatggttgg aaggtccaca
     6001 atacaatatt tcttcaacgg aaattcgcca aaacattaaa caaggtaaag aagtaaaaga
     6061 tttgcttcaa cctgctgttt atcaatatat ttttaaaaaa aagctatatg tttaatgcta
     6121 atttttctct cccaaaattt cttttatttc attataactc aaaacttctt tttctaaaag
     6181 tgtgatggcc attttctcca ataaatcttt tttttctgac aataattttt ttgcattttc
     6241 ataacatgag cgaatgattt cggtcatttc ttcatcaatc atcttttcaa catattccga
     6301 acgtcgttcg ataccgggtc cgccacccaa aaaaccggta gttgactgat ttacgaggga
     6361 aatattaggc aattttttac tcattccata aactgtaatc atgtttcgag ccaattgagt
     6421 agctctttcc aaatcgttgg aagcgccggt tgacacttca ccaaaaatca attcttcagc
     6481 agctcttccg cccaaaagtc cttttatttt tcctatcaat tcactttcag agagtagata
     6541 tctgtcttcc aaaggcattt gcatggtata tcccaaagct cctatacctc ttggaactat
     6601 tgaaactttc tgaacctcgt ctgctcccgg agtgaagtat ccgacaatag catggccact
     6661 ttcatgataa gctacaattt ttcgttcctt ttcgtttata agtttattct ttttctccaa
     6721 accggcaatt acacgctcaa tagcggcttc aaaatccatc atggtaactt catcacgatt
     6781 gtttcgtaca gctaaaattg cggcttcatt acaaacgttg gcaatttcgg cgcctgcaaa
     6841 tccgggcgtt tgtgcagcta atctctttaa atcgacatcc gaagccaatt taatattttt
     6901 tgtatgtact ttaaaaattg cttctctccc attcatgtcg ggtttatcga ccaatacctg
     6961 tcgatcaaaa cgtcctgggc gcaacaatgc cggatccagc acatctggcc gatttgtggc
     7021 tcccatgata attactccgg aactggcatc aaatccatcc atttcaacca aaagttgatt
     7081 cagcgtattt tcccgttcat cataaccgcc cgcataatat cccgagtgag ctctgctttt
     7141 accgatagcg tcaatttcat caataaaaat aatacagggt gcatgagcct ttgcctgatt
     7201 gaacaaatct ctaactcggg cagccccaac ccctacaaac atttcgacaa aatcggaacc
     7261 gctcagacta aaaaatggaa catccgcttc gcctgctact gctttagcca acaaggtttt
     7321 tcctgttccc ggagggccaa ccagcaggac tccttttggt aattttccgc ccaaacgtgt
     7381 atattttgaa gggtttttca gaaaatcaac caactctttt acctcttcaa cggcttcgtc
     7441 aatccctgct acgtctttaa aagttattgg atttgcaggt ttttctgcct gaatttgtgc
     7501 tttgtttctt ccaatttcga gcatggggtt tttgttgccc atttttctga aaaaatatcc
     7561 ccataacagc ataaataaca caaagggtaa aatccagttg agaaaaaagt ttaaaacagt
     7621 attgtcttga aaatgacctt taaaattaac ttttgccgcc aacaaatcat taatgagata
     7681 ttcttcattc atatttggaa ttctgctgac aacaaattgc ttttgtactt gttcttccaa
     7741 atcaggaatt ctgattctcc acggggcagt gggagcttgt gtcattgagg aaatatggtt
     7801 ggtatccgat ttgaattctc caactatttt atcctgtaaa aaaataattc tctccacttt
     7861 gccatttcgt accgaatcaa caaactcgtt atagcttaat tccctgttcc cttgtgtggt
     7921 cggaaaaata agccaatcca tcaaaataaa caaaaacatg ataaccagcc agtaaatcca
     7981 tgaatatttt gcacgttttg tcattcaaat aaattttttt aggtttataa aatttcgacg
     8041 aaaattagaa aaaaaaaacg ggaaactcaa tgaaaaaccg attttattat ttgatttttt
     8101 tcatttatta ttaccaaaac acaaaaaatc ccgacttttt aaatttttga gccgggattt
     8161 ttaaaataat tcgttaaaaa acaattacaa aaggctccat gctacagtga gacctgccat
     8221 tacgatagaa accatgctca tgagttttat taaaatattc aaggaagggc ctgaagtatc
     8281 cttaaaagga tctccaactg tatccccaac cactgcggct ttgtgatttt ccgaaccttt
     8341 accgcccaaa tttccttctt ctatatattt tttggcattg tcccatgcgc caccggaatt
     8401 ggccataaaa attgccagaa tgaaaccggt agaaacactt cccagcaaca atcccatgac
     8461 acccgaaacg ccaaatatca cgccggtaag aataggaata atgattgcca acaaggaagg
     8521 aagcaacatt tccttttgcg ctccttttgt agagatggct acacatcgag catagtcggg
     8581 ttcagctttg ccttccatga taccggcaat ttcgcgaaat tggcgacgaa cttcttgaac
     8641 cattttttga gcggcacgtc cgactgcatt cattgtcagt ccacaaaaca aaaatgcaga
     8701 cattgcacca ataaatgctc caactaaaac tcgaggattc atcaaattaa tttggaaata
     8761 ttctattaat tgtggaatag aagcttgatg aatatcggtc tgcatggcat tgaaaaataa
     8821 ttctgttttt tcgggaatac gagcaatggc aattttgatt tcttccaaat aagaagccat
     8881 caatgccaaa gccgtaaggg cggcagaacc aatggcaaat ccctttcctg tggcagctgt
     8941 agtatttccc aaagcgtcca gtgcatccgt gcgtcttcta acttcaggag ccaatccgct
     9001 catttctgca tttcctccgg cattatcggc aatagggcca taagcatcag aagccagcgt
     9061 gattcccaac gtagaaagca ttcctacagc cgaaatggca attccataca atcccatgct
     9121 gatattggaa aaatcgaaac ccgaagcaaa cagataggac aaaattatag aaatacccac
     9181 caccaaaacc ggaaaagcag tcgaaatcat tcctgttccg ataccggcaa taattaccgt
     9241 agcaggtccg gtttttgacg attcagaaat tcgctgtgtt ggtttaaaac ctgccgaagt
     9301 gaaatattcg gttatttttc caatggcaac acctccaaaa agtccagcca gcatcgatcc
     9361 ccaaatgccc aaataattag gtatattcaa atatttcaaa ataagaaatg aaaatatggc
     9421 aatgaaaatg gcgctgaaat tgattccacg acttaaagag cttaacaatt gcttttgaga
     9481 agcatcttct ttggatttta ccataaatat tccaataatt gatagaataa ttccaacggc
     9541 tgcaataatc atgggcgcaa ttacgccatt aaattgaact gctgttcctt ttgccgcaaa
     9601 agccacagct cctaatgcag cagtagaaag tatcgatcca caatatgatt cgtacaaatc
     9661 agcccccata ccggctacat cgcctacatt atcacccaca ttatcggcaa tggtggcagg
     9721 atttcgagga tcgtcttccg gaatattttc ttccactttt cccactaaat cggctcccac
     9781 atcagcagct ttagtaaaaa ttccaccccc aacacgtgca aacaaagcct gtgtagaagc
     9841 tcccattcca aaagttaaag tagtagtagt gataataatc agtttcatgg aagaatcgga
     9901 agctatagtc aaatttgtag cattatccaa tgcaccaata acaaactgca aaacataaaa
     9961 ccaaatggaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcaaaaca
    10081 taaaaccaaa tggaaatatc caataatccg agcccaacga caaccaatcc cataacggct
    10141 ccggaacgaa atgcaagtct cagtccggca ttcaaacttt gctgagcagc attagcagta
    10201 cgggcagaag cataagtagc tgttcgcatt cctataaatc cggctaagcc cgaaaaaaat
    10261 cctcctgtca gaaaagcaaa tggcacccac ggattttgta ttttcaaata ataggccata
    10321 aaggaaaaaa ttatagttat tataagaaat acaatgccta ctactttgta ttgttgtttt
    10381 aaataggcca ttgcaccttc ccgcacatat tttgctattt tttgcatact aatggtacct
    10441 tcactttctt ttttcatctg atgaaaaaaa taccacgcaa aaaataatgc caatacagca
    10501 gaaacaggta ccaaaaagaa aactttattc tccataacgt attgatttta agtatattaa
    10561 ttaaaatttc gaaatttatt cattttgata aaaaattcaa atttaaaatt attgagcatc
    10621 cgataacttt gttagccttt gatataaagt aggatgtgaa taatgaaaaa aagcatacaa
    10681 aggatgaggc atcaaattgc taagtgaagt ggcagccagc ttttttaaac cactctgcag
    10741 ctgctttgcc cagccaaaat ttgcagcaaa agcatcagcc tgatattcga attttctgga
    10801 taaaatattg gtacaaatac tcaacaacag cgaaacaggt gtatataaaa ggccaaaagc
    10861 aatgacgttc aaatgaaagg aagcagttgt tccacccaaa acttgagcaa aaacatcact
    10921 tttcagtatc aatcccaata ggaaaaatag taaaagactt tccggaagag aaatcagaaa
    10981 ttgataaagt gtgtgtttct ttttataatg accaatttca tgcgccaaaa cagctactat
    11041 ttcttcagaa gtaagcttgt cgagcaaagt atcgtaaagc actactcttt ttttgtttcc
    11101 caaaccggtg aaataagcat ttgcttttgt cgaacgtttt gaactgtcga taatataaat
    11161 attgtccaat ttgaatccgg ctttggttgc aaatttttct atctcatcgc gtaattctcc
    11221 ttcttcaagc ggcttttgct tgttgaaaat aggtacaatg atttgtgaat aaaacatgga
    11281 catgaacaaa ctaaacaagg taacaaccga ccaagcaatc aaccagaaat actttggagt
    11341 taattgatat atccaaacta ttgcgcctaa taaaatgcct cccaaaaata cacttaaagc
    11401 aaattccttt aaatgatcct gaacaaagag ctttggtgtt accttattaa atccgaattt
    11461 ctgttctata acaaaagtat cgtaccactg aaaaggaata ctgataagtg tttctgcaac
    11521 aaaaagcact ccgaaataca acaacggcaa taaaatggga tgctcggtta ttcggcgcag
    11581 aagcgtatcc agccaaccaa atccgcctaa acagaaaaaa attaatatta taataaagtt
    11641 aatggtggaa gaaatggttc cgaaacgtat attggatcga aaatattctt gctgtttacc
    11701 gtatttgtcg gcgtcataaa tatcttttaa attatcggga agttcatttt tggaatattg
    11761 aaaattcaaa aaagacaaat aacgctctaa aataaaatca cctactataa agaataaaat
    11821 caaataaaac aaaatttcta ccagtgacat catctttcgt ttcaattatt taaattgagt
    11881 gcaaatataa ttaatttaat gatttcccaa ccctttgggc tcaaaattta cataaaaagc
    11941 atatctttgc ctttccttta taaaaatttc aatttgtatg ctgtcagtca tggaaatcct
    12001 taaatatatg aagagattac aaatattttt atttattctc ataactcttt tttccctttc
    12061 ctgttccaat aaaacagctt ccgataaaat cagcattgcc atcccggaag gtccttcagt
    12121 tgtcagtttt ataaaaatga tggacgattc cgtgtcaatt gaagggaaaa aagtggaata
    12181 tattgtaaaa tccgacccac agcaaattca agccctcatg atgcaaaaca aagtggactt
    12241 tgccatttta ccaaccaata tggcggccaa tcttttcaac aagggcatta aatataaaat
    12301 gatagcctgc cccgtgtggg gaactttgta tattttgtcg aacttgcctg aagtaaaaac
    12361 tttaaacgat ttgcaaaaca gaaaagtaag catttttggt cagggcacca cagccgatat
    12421 cctgtttcaa aaattgatcc agaaaaaagg aatagaaaaa gtttttattg attatcgttt
    12481 tgcgggaaat tttgaacttt cccaagcgct tcgtttaaag aaaaccaata tagccgttat
    12541 ttccgaacct ttggtaagta aacttttgga acaggattct accattcata tcgtagaaaa
    12601 attgtcatgc gaagagtatc taaatgaaaa agatagagat atattcgtac aaactgcttt
    12661 tttagtcaat gaaaaattgg aacggaataa tcctcagatt ataaaggaaa tatgtaatgc
    12721 ttatcgtgcc agttgcaatt atgttactca acatccggaa gaagctgccg ttttactggt
    12781 taaacatggc attttaccgg aaacaaatgt ggccaaaaag tcaattttgc tgtgcagtat
    12841 caattacgta gctgcctttg ccatcgagga tgaaatttac agttatctga aaatttttta
    12901 cgacttcaat cctgaaagca ttggaggaaa attaccttca gaaaatttca tttttcaagt
    12961 tttttagcct tgacggatat gaaaaatcag aataaaaaaa tttttccttt tattctttct
    13021 gtgttttcgc tggcaattat ttggcaaatt atttctatat ctattggtta tcctgctatt
    13081 tttccctcca tttttgattt aatatcagat acgttacatt tatttacccg ctccgaattt
    13141 tattttgcat tattgggaac cgtcagcaga gggttattgg gttttgttat cactttcttt
    13201 attgctctct tttttggaag tttaagtgtt ttcaattcat ttttcaagga tttttttcat
    13261 cccattgtgg tttttacgcg ctccataccg gtgatatcta tcgtgcttgt cgctcttttg
    13321 tggttttctc cccctttttt gcctgttttt gttgcatttt tcagcatgtt tcccattctg
    13381 tttcaaagta tccaaagcgg attggaaaat gtggataaaa aatgggtcga aatgggcaag
    13441 gtttttggac tttcatctcg aaagcgtttt ttacacattt attatcccgc cgccagatcg
    13501 actatttttg acggtatcag ttctgcaatg ggatttggat ggagagccgt aattataggc
    13561 gaagcactgg ctcaacctgt tcatggcatt ggttcgcaaa tgaaagctgc tcaggcttat
    13621 attaatgttt ccgaaattat agcatggaca cttgcagcca ttttgataag ttatattttc
    13681 gagaagataa ttctttttct gaaaaagtgt aatcgtccca ttcaactacc caaatcagaa
    13741 gaatgtaagt tgcataaatt caatcaaata gttgataaaa aggaaattac tatttataac
    13801 ttaaagaaaa aatttaatga ttatactgtt ttttcgtctt tcaatgaaaa ctttaaggac
    13861 accgaaatta attttatcaa agggccatcg ggcattggaa aaacaacttt attgaggttg
    13921 atagcacgac ttgaaaaacc cgacgaagga acgatttatt attcttcaaa attcagtttt
    13981 ggatattcgt ttcaagatgt tcgtttattg ccttggctta ctgttaaaga aaatatcgca
    14041 tttccactgt tgtataaaaa aacagataga caagaactca ccgataacat cgattggctg
    14101 ctaaataata tggaattgac agaacatcaa aacaagtttc ctgatcaatt gagtggagga
    14161 caattgcaac gcgtcggatt ggctcgcgct ttagttgcgc aacccgacgt tttgcttttg
    14221 gacgaaccgt ttacaggttt tgacgacgat ttaaaaacaa gaattcaaga tttcttatcg
    14281 aaatggtttg aagattttca gccactcgtt atttgggcaa cacatgaaaa tattctcttt
    14341 ccggaaaaaa atagaagaga aattcaactt aaaaattgaa acgatggata tctgctgaaa
    14401 acatcgaatt tggaaattaa acaaaagcaa caattttgtt gggatagttg ttaaatttac
    14461 gttgatttga ctgatgtaaa taattgctac agctccaata atccttcggg caaatccatg
    14521 taaataattt ttttcatttc atcaatttgt agaatgtaat cttggcaagc tggaatcagt
    14581 atctgctttt cgccattgtc aactataaaa aggacgtttt gtgtactggt atcgatatct
    14641 gtaacgatac cgatgaaatt attgtcttta tcgaatattc gaaattcgat aaatgtattg
    14701 ttatccatat caacatcact ttccaagata ttcaaaaatt ttttatgcaa ataaatagtc
    14761 aatcctaaaa aattttttgc ttttttttca ttgttaacat cttcaatttt taaaaagccg
    14821 ctattttcag aattgaaacg ccattctttc aaataaaatg gaacaaggat gccatgcata
    14881 tcgaaaatca aaaatgggat attatcaaaa tccaatcctt cgtaattgga catgaaagcc
    14941 atttccccgt taattccgtg taatttaatt atttgtccta tgggtataac ttctgtaaaa
    15001 gaaatcatct cagaaaaatt ttaaaaatcc ggaaaaaaat ttttttcaac aaatcaagcc
    15061 attttccgaa aaatcgtcaa aatgaaaagg ctacacccgt catgaggttg aatccttgaa
    15121 catcgtagcc ataaaaatac tgatatttgt tgttcagaag attattcaag tgtaaataga
    15181 ctgaaagcga cttgctgaca atataggaaa ttcccaagtt aacgtccatt atgttgtcca
    15241 ttttcactga acgattgcct aattgagcat atctgccggt tttgtaaaag aaataaaggg
    15301 atgttgtgat atttgaagtt attcccagat tggctctcaa atcagtttcc catgtcggct
    15361 gtaaccaggg caatgccgtg tcttttgtat cccaccaatt ataagtggtt cgaaattcca
    15421 tgttaaaacg gttcttttcg tcattgtatg ccgccctgcc tccaatttta aaatgatagg
    15481 catcgctgta agcaacattg aaccgattgg taaataaaac cgaatcggga gcattcaatt
    15541 cttcggtaat gtattccttg tttacataaa aatattgctg atcagtaaat cgataatcgg
    15601 cataaaaatc gacgagtaaa tttttagcag gttttatttt gacacctcca tagctgagaa
    15661 aaggggtgta agtgtctttt attcgtgtgt cggaaaatag gaagcaattt tccgaaaaaa
    15721 ttctgtccat gctattgatt tcataatcgc ccgaaagacc accatacaaa gtcatccact
    15781 gaggaaaaac attccactcg gcagcaatat cggctgcagg actgaaagaa cgcccatgtt
    15841 tcattgaaaa agcggctttc agtcccattc gtaatttcca attggtttgt tctatggaat
    15901 agaagggatt aagcataata acggaataat aatcccaaaa gttgaataga ggagcttcat
    15961 tagcggtata ttttatttga tgaagttgga catccaagcc aatattgttt tcgtgaaaag
    16021 gcataataac attgcctgta agatgtattc ggttttcttt gataccaaat ttattttgaa
    16081 agaaatgata attcaatttg gcagaataat tgatatcgga agttaaaggt aatgactgca
    16141 aacctccgaa tgcattgaga tgccagaaag tttccgtacc gggagtttcg gacaattctt
    16201 gtaaggtaaa agaatgaggc gcacgggtga tggaggtaaa ttgccgttca atataggtgg
    16261 atgaaccatg attttttgct aattcattta gttgaacaga ggcggaatcg ttaaaattaa
    16321 agccataaaa tttcaatctt tcatgttgag tttctactcc ggcaaacaaa tgtaagcttt
    16381 tcaattgttg gtcaaaaacc aatcccgatt tggttgttga atgaagtttg tcgccgaaag
    16441 ttccaatatg attcatgtaa aagtcgagct ttgtattggg acgattaagt aaagaatata
    16501 aaaaatttgc ggaagaatta tagaagtttc ccaatcccaa ctgtaaaaaa ttgttttgtt
    16561 ctaccctgtt attaaaaaaa aactcagttg acgacaacgg atgaatattg ttattaatgg
    16621 aaaggggaat aaaaagatca gtatattgag ccggtagagg ttttgcttcg ggttcaataa
    16681 gtttgggtgt aacatttaat tttccggtat tttttagtat aggttgatat tccctttcaa
    16741 ctgtaatgtt tcgggtgaaa acagaatcct gagccaaaga tatggcaaaa tttacacagc
    16801 tgaatataga taaaaatact aattttttct tcaagtttat catatcaatt tacggtttgt
    16861 gaaaataaat acaattttgt ctaaaaaaaa catttttgtc aatcaaagag ttattcctaa
    16921 aatgtcaatg aataatattt ttcttttctc tttggctaat agctgacaat cgctcatcaa
    16981 tcaaaccctg aatttcatcc tgaatggtat aatttctttt taagcttaaa aggtattgtt
    17041 ttgcttgaaa atcattccct ttttttatgt atatatctgc cagcagtaca aaactacgag
    17101 ccaaccaaaa ttgatgaggc gtattttttt ttgcaaaatc caaaatttca ttttctgctt
    17161 cagtcagttt ttcttgttga aaatataccg aagctaacaa gtattttgat tctgctccaa
    17221 aagctgtttt ggtatcagca gaaataattt tcaggtcttc aatggctgca tttagctgtt
    17281 gatgaagaag atatgcttta gcacggtaaa accttgcttc ggtctttaca atagcttccg
    17341 aatatggatc ttgcaaaatt acattggcaa tatcgacaag actttgataa tcattagtaa
    17401 agtaactaca acgtaacatt cccaaatggg cagcctgttt attagccgaa ttctgagcta
    17461 cttgtttaag ttgtttgaag taattgaagg cagatgcata gtctttctgg tcataggtaa
    17521 tttctgcaca atgcagggca gcttgttcaa catattcggc agcattggtt ttcagtacaa
    17581 cttcataggc tttcagtgcc ttatctcttt gctgtgtgcg ataatagctg tcagccagat
    17641 agaattgagc aatggtacaa tacctgcctc cgggacaaaa attattcaaa taattttcaa
    17701 aacctgagat agcaacagta tatctaccat ttatgtattg tttttcggct gcaatgtaag
    17761 aaatggaatc ttcttgcata accgataagg gacgaactat acgccccgaa tttttggtat
    17821 attgcaaaaa accattcaca tcgtttaatt ctatatacgc actttccatg cttttaagag
    17881 cgatatatga ttcttcggtg tcgggatatt tttccgctac atacttgaaa ataacaatcg
    17941 cattttcaaa atcttgtcgg ttgaaataaa tcataccact ttcaagtgct gcttttctgg
    18001 ataatacaga tgaaggatac tgattcatta atcgctcata agcagacaaa gccatttttt
    18061 cgtttttaag catcaaataa gaacgaccaa tctcatagag ggcatcatcg ccatactctg
    18121 aatcgggata tttcaacaac aattgctcga gacgataaat tttgttcaaa taattttttt
    18181 gtaatccttc tacgtatgct gcctgaaaaa tagaataatc cgctgtattg ggagatgtaa
    18241 aagcagcttt ttcataaaag gaaagtgcat ttttaaaatt tctggcataa aaatgacaat
    18301 ctcctgctcg agttaacgca tcagcataga gcattgattt ctctgaattt tcattttcaa
    18361 gatatttgga aaaccattcc aatgccttga tataattttt ctgatcaaaa tatccataac
    18421 ccaaagaata ataggcatat attaaatttt cgctgtttct ggacaaggga tttttaatga
    18481 atgtttccaa atctacaatt gcttgatcgg gattgttggt gcgatagtag cattcggctc
    18541 tccagaataa gcattcggct gaatattttc ctttgggtgc agatgtatgg gcaagagaaa
    18601 aatattcgat agcttttcca taatccttct gagcaaaggc attgacgccc atctgataga
    18661 gaagatattg tttggtctcg gttaaagatg tatttaattt ttttattttc aaaatagatt
    18721 gataagccag ttcataattt tttgtagtta gataaacgga agtcaaatat tgataagcct
    18781 cgtcagcata tttggagttt ggaaactcgg aaagaaaacg ggtaaaagct gcaaccgatt
    18841 ccccgaatgc tgaagttgtt tcataacaag ttaaagcata attaaataaa gcttcttctc
    18901 gaacagttgg attaaagtgt gtttgcaaag cggcttcaaa agaaaggcgg gcattggttt
    18961 tatcgttcaa gaaaacatat gatgttccca gatgaagata ggcattttcg gaaatttcat
    19021 caggttcggt ggtaactttt gatagatatt ggatggcatt ggaataatct ttcagttgaa
    19081 aataggacaa tcccaataaa taaacatcgt ttctgagaac tttaggggac agattttcat
    19141 actttttgaa ataagatatg gcttgtgcat aattttgttc gccataagcc aattctccaa
    19201 gtattcggaa aatttctgca ttgttattat tgttgggata tttttgaagt agaaagtcag
    19261 ctttttcttt caattttgcg tattgttttt caacataata aatttgtacc aaaaaataag
    19321 gaacgctaat ttgatagtcc ggatgatttt ctatagctaa aaaatcgggg agggctttta
    19381 ggtaatttcc catcatatat tccgaatagg catagtaata tgttgcatcc acacgaaagg
    19441 gagaattctg aagttttaat cgagaaaaga tttttccggc ttcttcgtaa tgttgtgttt
    19501 gaattgcggt atagcctttg taaaaaagga attcgggaat tttattttca gacagctcct
    19561 gttcgtttac ttgagaaaaa ttttctctgg caactgtgta gtttttctgt tcgcagttaa
    19621 gtaccccaat cataaaataa atttcctcag aaaaaggtgt ataaggatgt ttggtgagaa
    19681 aagtctgtaa aagtgaaaga gcgttttctt ttctaagttc gtatgcattg gccgacagga
    19741 aatattcggc ttcctgaaac tgtccatcag agggaaatgt ttttttttct aaaaactgtt
    19801 caaaacttcg gtaagaggca gcgtattttt gttgaaaata aagttctttc ccttgaatga
    19861 aatctttatc ttcggaggca aaaaataagg tgtgttgtgc ggaaagaaat aagttgctta
    19921 aacaaagaaa aagaataaga gttaaaaaag gaatattttt ttttgccatt tttatagaat
    19981 ttatttttta caaacaaaag agcataatta agcaggacaa aatgtggaaa tttattgcaa
    20041 aattaaatgt tttgacactt tttgccaatt tgttcccgta gaaaaatttc aactgctttt
    20101 ttaaccgatt tcaaaccaaa aagttcaagt tgaatttttt caagaggcac ataaaagcag
    20161 tcttccacat catccgaagg tatgggtatc aggtcttttt ttatttgaca gagaaaaaac
    20221 aaatcgagcg taggaataag caaattgctg tattcgtatt cgttaggtaa ggagaaaagg
    20281 taagttggat tttcaacttc aagctgtaat tcttccatga tttccctttt caatgcttct
    20341 tctgctgttt cataattgtc tacaaagcct ccgggtaaat ccagtgttcc tttggcggga
    20401 tctttttttc gacgacaaac gagcaactct tttttttcgt ttaaaataaa agcagccact
    20461 gctgctgatg cattcaggta ataaacaaaa ccacaagcct tacaacgttt tgatttttcg
    20521 ttgtttatct caaaatctgc agaaccacat gaaggacaaa aagaaaattt cgaaaaaaaa
    20581 tccattactt gttctttccc ttgatatgtt tttttcattt aaagaatatc tttaaaatta
    20641 aagattacag ttgaaaattg taggcatgtc gtacaataaa catgccattt gataattnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt gtaattgaaa aagttgttta
    20821 taactttgtc ttaactcgtt tatttattga atttaaaatg ttatttttgc aaaaaaatta
    20881 atacgtatat gggcaaaaag ttattgcttc ccggcgatga tgagttcaaa gaccttgttc
    20941 acaggtatga gcagtacctt tcgggcaatg caaatggata cttcgatgtg gaagaatttg
    21001 aaaatattgc ggaatactat ttgctgagag gaagaaccaa agattcttcc agggctattg
    21061 attttggcat gaaattacac ccgggcagta cagcactcaa agctaaacgg gcaaaaatat
    21121 atttggctgt tgatgaagta aaaaaagctt atcaattgct ttcatctttt accgaaagcg
    21181 attacgaaac cttattgttg aaaattgaag cccttgttcg catgggtcgt catgctgaag
    21241 ctgataaatt gataaaaggc ttgacaacag aaaaaacagc cgatttggat aatatctttc
    21301 tcgatatagc ttatatttat ctcgagaaca ataaatttga gaaagcttcg gaatttttat
    21361 tgaaaggctt tcaaattaat ccaaagaata tcgatttact ttttgaatta gcttttagtt
    21421 ttgaacagat ggaagaaaaa gaaaaagcta ttgaaattta taaccgtatt cttgatttgg
    21481 atgctttttc cggagaagct tggtttaata taggacaaat ttatttcaat ttgcaggaat
    21541 ttcctaaggc tttagaagct tatgattttg tgctggctat tgatcccaat gacagtctgg
    21601 cctatctgca aaaggcacat gttcattatc agctcgaaga gttttatact gccattgaat
    21661 attatgccca ttatgctgcc atgaccgatg acccagcttc gactagcctc tatattgccg
    21721 aatgttatga aaaacttgag cagtacgaca aagctgtaga ttattataaa tatttacttg
    21781 aacaagatcc ggataatacc gacctgctta tttccattgg atcttgttta ttggaacaag
    21841 aaaaattctc agcaagcatt gaatatcttt cacgtgccat agaacttaat gattcattgg
    21901 ccgatgcttg ggtttatctg gctgaagctt atttggccat cgataatctg gatattgcat
    21961 taaagtctta tttgcttgca cttcagctcg atagtgagca gcctgaagtt ataatggctg
    22021 tagcaaatat ttatttggaa aagggagatt acgagactgc attaaatttt tatctggctg
    22081 ttgaggcaat gagcaattca ttggaaattg tcaatgtttt tattgctgcc acttattata
    22141 aattggggaa taagaccgaa gcttatcgtt acttgaaaaa aggaatagag gataatgaag
    22201 atgtgctaaa tctgtttttg gaaatttgtc ccgaagctat cgagacgatc cattaatttc
    22261 ctatagatgc agttattcaa aaattttttt accgcgtatt gtaatttttt tttatgccgg
    22321 tggctacctt atacttaaga aataaatttt gatgaaaatg agactgtatg ggttgattgg
    22381 ttatccgcta aaacactctt tttcaaaacg ttattttgca gaaaaattta ggagggaagg
    22441 aattgatgct cgatacgatt tatatgaact tcaacgcata gatgatttcc ctgaactggt
    22501 caaaatgcct ggtctttgcg gcttgaatgt aaccattcca tataaggaaa gcgttattcc
    22561 ttttctaaat gaattggacg aagtggctga aaagataggg gcagtaaatg tcattaaatt
    22621 tttgagaaaa aataatcatt tgtggctcaa aggctataat tcggatatta ttggttttga
    22681 aaaggccata attccttttt tgaaaacttt tcatgacaaa gcattgctac tcggaaccgg
    22741 aggagcagcc aaagcgattt cttttgttct tgcagctcat aatattaaag ttactcatgt
    22801 ttcacgaaag ccaggcatca ataaaataac ctacaatgat ttaaacgaag agttgatgcg
    22861 tgagcataaa ttaattatta atgccacacc acttggaacc tatcctaacc tttgctcatg
    22921 ccccgacatc ccttatgaac atcttacgcc acaacattta ttgtttgatg ttgtttacaa
    22981 cccacctaaa actttattct tgaaaaaagg ggaagaacaa ggagcgacca taatcaatgg
    23041 cgaagccatg ttgagaggac aagcagaagc tgcatgggaa atatggaaca aatagaagat
    23101 agtatagttt attttttcag agcaagagag gaaggactct ttcccctttt gctccgatgg
    23161 aaaaaaaatg actataattg ttcaccaaac gttttgatag gcaatcatta aaattatgct
    23221 gatttttttg attatacttt ccttatttat tgggaaaata tttattgaaa aacaatatca
    23281 tatcctgcca tgaagcctta tcggctgctt cgttatatgc aatgggcata ttgaattttt
    23341 tccctgcttc agtagaatat ttattggtaa aagcatgagt agcaccttca taatttttga
    23401 attcgtaagt tatattgtat ttttgcatct cacttttaaa attatctata tctgcttgtg
    23461 taacaaacat atctgctgct ccattacaaa taagcacttt tgtgtttttc atcgccggac
    23521 tggctttaaa gccattgagc ccaccatgaa aacttacaac cgtttccaaa ggcagtccca
    23581 ttgcagcagc atttagcacc actgtgcctc caaaacaata gccaattgca ctaacatgtt
    23641 ttttatcaac agttttttct gaagtcagaa tttcataacc tttttccatc cgctctttaa
    23701 gtaattctgg atgagaaaaa atatatcccg ctgcgttttg agcttctgca gcagtatcat
    23761 ataccttacc gtctccgtac atatcaactg ccatagccac atagcctaac tctgcaagca
    23821 tgtctgtacg catttttatg tattcgttaa atccccacca ctcatgcaca accagaatgc
    23881 cgggttttgt tttttttcca tcatctttcc atgcaagata tcctctaaaa ttttgatcgc
    23941 caattttata cgaaatactt tttgtttgaa tatctgattt tttgtttgag ttgttgtttt
    24001 gagctgttcc aaaaacaggt aataacagca tgatgccaaa cataattnnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24121 nnnnnnnnnn nnnnnnnnnn nnnnnnnttt attatttata ctttgattga ttgatttgat
    24181 ttttgaaaat atataaaatc tattaatgag aatagaaatt gttgttaaat ttcacatcca
    24241 tcggggccgc aaactccgcc attttcaccg gaaaactcaa taatatcggt ttttttgtgt
    24301 tcagctaaag ctctgttgag cgcttgtaaa aaaaccgatt catcctgtgc tcccgaaacg
    24361 gcaaatttat cgtcgaacag aaaatatggc acacctctga cgtagagctg atgcgcatgg
    24421 gcaatatcgt gtttgacttc atcgctataa gcatccgaaa gtaatacttc gtttgtttcc
    24481 gtagcttcca aacccacttc ctttgccaat tgaagtaaaa tttcattgtc atcgatattt
    24541 ttcccatcag taaaataagc agcaaaaagt ttttcttcca tttcgttttg cttgccgtgt
    24601 tttttggcca gatgcaataa tcgatgagcc ttgaaggaat ttactacaat tgccttttca
    24661 aaatgaaatt ccaatccgac ttccttagcc atctgtgtaa tgaaggcagt ggattggagc
    24721 gcttgctcca ttgaccaacc tttttcggca gccaaatgtt ccattgtttt caactcagga
    24781 ttggtaacgg caaaaggatc gagttgaaaa cttttccatt ctacttgaac attttccctg
    24841 taagggaatt tttcaagcgc tttttcaaat ttatgtttgc caatatagca gaaagggcaa
    24901 actacatcac tccagatttc aactttaatc atgaaattat ttttattttc catttttctt
    24961 attgtaaaat tattgtcagt tttagcggat aagtccggga actttttcgc catgaataaa
    25021 ttttaatatt ttttgtaccg catgttcagt cccaatcagc aaggcattgt gaaccatagc
    25081 cgaattgtgg ggtgaaccca acaagttggg taattcaaaa aacggataat gaagttcaaa
    25141 ttttcgatcc ttaaatggtt ctacccacca ggcatcgatc cccgcataaa aattcggatg
    25201 tgttttcaga tgttcataca acgcagcttc ctcaacaatc gcaccgcgtg ccacattcac
    25261 cagcacggca tcttctttca tcaattccaa tctttctttg ttgatcaaac cttctgtctc
    25321 ttcattcagc gctatggaaa ggatgagact atcagcattt tgcagaacat agtccaaatc
    25381 tttcaaggtt ccaataaaat cgacctcctc atccgtttta ccggaactgt tgatcgccat
    25441 taacttcaca ccaaaaggtc gcaataatct ggctgtagct tttccgatgg ctccaaaacc
    25501 aattattccg gcaaccgaac ctttcagaaa tttggtttga ctttttatct gactgaaatt
    25561 gccctttgcc aagtcgttgt gataaacata aagccttttg ctgagcgcca acaacatagc
    25621 catcacatgt tcggccattg gttcggcata agctccctga ttggcagcta tctgaagttt
    25681 ttcaggaaaa tcttccaacc gaatatgatc atatccggca gaaagtaatt gcataaacct
    25741 gatattatcc ataggcaaat tttgtttgtt aatgccttcc tgcaccggat tccatgacaa
    25801 aaggacatcg gctttagtca tcagattttc caattcttct tttgtgcaat cttccttaaa
    25861 gtaaatttgc atttcctttc ccagtacatt ctttaaatac tctttttgct ccggaaaagt
    25921 tttaaaagtt actattatag tcattttctt ttttatcatt ttatttgatt gatccacttt
    25981 tcgagtgttt cataaggctg tgcaccggtt aacttgtatt tgccgttggc aatcagtgtg
    26041 ggtaccgaat tgatgccata ctgataagca agataaaagt cctcatcaag catttgtttt
    26101 actttttcgc tgtgaaaatc tttctcccat tgttcgacat tcaatcccac ctcacgcgca
    26161 cacattttta gcacttcaac atcattaata ttaagacatt cagtcaaatg agctttctga
    26221 atacgattaa atattttgtg atgcatttca tttccaccct gcaattcggc agccttacaa
    26281 gccagcaatc cccgagtaga atacggatag tcaaaagttc gggttgccat taattcggga
    26341 tgcatgcgat gagcatcatc attcatgttg gcagctctcc aatgccctaa aatttgtatt
    26401 tttccttcct ccttcgaacc gaaaattttt tctatcccat ttggttcggg agccagtgca
    26461 aatgatcgat gaataacctc aatgcgatca aaatatttct ctactaattt ttccactctg
    26521 ggcgaaagtg cataacacca tgcgcaaagt acatcgtgga aaaattctat ggtaatttta
    26581 ttttgttcca taattttttt aaccaaaacg atacgacgaa attttaactt tcaaaagtgt
    26641 tcccgaaaaa tcctgtttgc ctaagtcgtt tccggcagct ccggcaacca caaaaagcgg
    26701 aaccaaatgt tcgcttcgcg gatgcgattc tcttgcaccg ggcgcactta accactgagt
    26761 aagaagttga ttacgaattt ccggattttt attttctact gcatgagtca accatttgtc
    26821 aaactgttcc gaaattttcg aaatagcctg attgcccgac atcattccac gcaaattatg
    26881 aaaactcatt ccggaaccaa taataagtac cccttccgag cgcaaagatt caagtgcttt
    26941 tcccatagca atatgctgag ccggtcgaag tcccttaatg agcgaaagtt gtaccacagg
    27001 aatatcagct tgaggaaaag ctaccattaa cggtacaaaa acgccgtgat caaatccgcg
    27061 aagattttca cgagctgttg taaaaccggc ttcgagcaac aggttttcaa tctttaacgc
    27121 cagttgagga tcgccgggag caggccagct caattgataa gtaaattcgg ggaaaccgta
    27181 ataatcgtac agcaatgacg gatgctcacc aaaattgacg gttggcaccg attcttccca
    27241 atgacctgag acgacaagaa tggctttaat tttatcatga aaatcattgc caattttttt
    27301 taaataatta cgcagcattc catatccctg agggtcgccc atgaagtcat ccatcacatg
    27361 ccatggccca cctccatgag gaataaagaa taccggtaat ttttctgaaa ttttcatgtt
    27421 gttgaatcaa tctgaaaata gacagattgt gtccattctc aattttactt ttctctgact
    27481 gtttttaaag cagttcccca agctatgagc tgatccagca tggcatgcaa gctgccttcg
    27541 tgtgaagaag caggtttaaa aacactgaaa ttttcaaaat ccgtaaagag tgaaagtgtt
    27601 acctgagctc taacgtcggc tatctgaatt tcacccatga tttggcgaag ctgttctaca
    27661 gctcttgcac cgccggcaga gccataactt acaaatcctg ctgctttgtt gttccattcg
    27721 gcataaacaa aatcgatagc attttttaaa gctgctgtcg gtgcatgatt atattctggt
    27781 gttacaaaaa catacgcatc catttcgttg attttctctg cccatttttt tgtatgttcc
    27841 ttttgatact gatgcatgga agccggaatc atttcatcga gcaacggcaa attataatcc
    27901 gcaatatcaa ctatttcaaa aattgcatca ttacgtttac tggctacctc ataaacccat
    27961 tttgcaacag cttccgagtt acggcccggg cgcgtacttc caactattat tcctattttt
    28021 atcataatct tttcattttt tactgtttat tttttcttcc aattcatcca gcctatcgtt
    28081 ggccatgtgc accatcatgg ttacccgtac aaaaggcaaa gccaacaaga gcaaagccaa
    28141 cgagataatc agttgaaacc agttgaaggc aacgatattt acaataattc cgataagtcc
    28201 aattaaagcg gcaataattc cacccggtat tccaagtgga catctgagtt ttttctttgt
    28261 ttccattttt ttgattttta attactgata attttttaat ttttctatca actcttcttt
    28321 cgtcggcacg cttttgaaca ccaccttgtt gtcaatcaac aaagtcggaa cggtatggat
    28381 acccaatttc atggttcgta tcattccgct tggtgaattg gcgagggtgg ttttcagtga
    28441 aaatttatcc tcaaactgag gtaaaacttc tttcagcatt tgcttcgtaa gtttacagtt
    28501 cggacaagtc aacgtataaa agagttcgat agttgttttt ttattcattt taaaggattt
    28561 ataaaatttc tttatgctta aaaagtataa atattactca cgacaaaaat aaaattgaaa
    28621 aaaatattct tgagactgta atttttttgt aaaatttgcc gttccttgta cttttcgtaa
    28681 gtctgttgtc tgcaaaatat ttttttataa ataaatttat ttcaaatttg tataaataac
    28741 atgcattaag gaaaaaagtt catgtttttt tgataaagca tttgccattt acaaaataaa
    28801 attgctgttt tttaaagcaa attattgtaa cgtattgtaa caataaaaaa tttaacgact
    28861 ttcttcagct taaattttgc ttttgctttg tttccatatt caaagtttaa ggagaaaggt
    28921 aaaaaatgta tttttattcg ttagttcaaa aattcaaaaa tatcccgtaa ttttgcaccg
    28981 caaaaaagaa attaactgca attgccttct tacctcgttt tttcttcgga agtataaggt
    29041 ttaaaaacag ctcaatatta ttaatggatt tggttggatt ttttattctt ggaatagcgg
    29101 caggaatttt gtcgggattg tttggcattg gaggaggaat cgttatcgtt ccctcgttga
    29161 tagtgatttt tggtttggac attttggatg ccaatgctgt ttcgctggca gccatgttgt
    29221 tgccggttgg aattttaggc gtatgggctt attacaaaga aggttatgta aatattggcg
    29281 agtcgctttg ggtagcattg gggttgttct tggggtcatt tgttggaggt ggaattgccg
    29341 taaatattgg cgaaagtttg cttgccaagt tatatgccgg aattttaatc tatattgcca
    29401 tcagttattt tcaattacca aagtattttc ggaaaaagca gccgatagaa aaagaagaaa
    29461 aaccacgcga aaaaaaatca ttctgggcat ttgttctgat aggtttggca gccggagttt
    29521 ttgccggatt atttggcaaa ggcggcgggc tggttattgt tccgatgctg attcattttt
    29581 ttcattacga cagtcgttct gctgctgcaa cttcattggc tgccttgcaa cttccggtcg
    29641 gcttgccaag cgtactgatt tatgctgaaa acggacattt gaatttgctg tttgccgcac
    29701 tgatcgcttt gggtattttt gtaggagttt taataggttc aaaaatcggc attaaaattc
    29761 caccatcaat tttcaaaaaa atctatgcgg tttttctgct ggcaattgct gtttatatgg
    29821 catttgaata tatttgaaac aattgatttc accgaatcga aatactttaa taaactttat
    29881 gattatccgc aatgattcct attgtaaatt cttgacttga aatcagtgtt gtagctcaca
    29941 gcagcaacaa gcaacctgtc gaagattgca tcaccaaaat aa
//
LOCUS       DAOTAX010000022        29256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1315_length_29256_cov_30.713914, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000022 DAOTAX010000000
VERSION     DAOTAX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29256
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_29256_cov_30.713914"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..3426
                     /locus_tag="PLM04_03945"
     CDS             424..3426
                     /locus_tag="PLM04_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02264.1"
                     /translation="MKNKFYLLLVLLSFPLLIFAQAQREVKGKILNENGEPVIGANIF
                     ESGNPTNGTISDLEGNFVIKISPQSAIEISYIGYVPQKIKDPKSPLTIQLEVDQRSLD
                     EVVVIGYQTVKKSDLTGAVSVFKPETMKNTVVTGTIGEALYSVPGIFVRSAGAPGNEG
                     FIQIRGTSTFGTSNPLYIIDGIDVGTANRDFNYNDIESIQVLKDASAAAIYGSRAANG
                     VIIITTKKGSKGKMKIDFSAKNTFQWLPQYNLTNREQWITLNDLAFKNAGRQPANHSD
                     GNTNWQDEVFKTGILQDYDLSFSGGGETNTYYLSANYQTNSGTTYGTNSERITARINT
                     SSFRDFGKNVRFTIGENLIISNYFVDELNTNPITDVWRMLPTIPVYDPSNPGGFGYGD
                     GSKDVTFGTNPIARENLENTTNENLRIRGNAFAELSFFKSLKYKFNLGLVTSSDSHMY
                     LRKVGNWTYNQPIDPSSLNRNKALYKFMVYDNTLEFNKTFGVNNIAAVAGTSFSTEDY
                     SQIWGTKNNVLTSGDDYFTQLDAALLDPKTGSYQNLAKLFSIFGRLNYSYDDKYILSA
                     TVRRDASSKFGPNYRNGVFPSIAGAWRISKEKFFNVSWIDDLKIRANYGILGSSNIGY
                     WDWVPFINIFPQAIFGAGQNINTGMTQVKLVNTDLKWEELHQTNAGFDAYLLNRRLEV
                     VGDYFNKTTQDVLTPMQILLTTGNNGGNPMVNAATLKNTGFEVSVNWRDRINDLNYSV
                     GLSTSYLKNEIVELGYGRESFTQWDTKSFVGKPIGDWYLIKTDGIFRSTDEVLNHINS
                     KGQVIQPSAKPGDIRFVDFNDDGQITDADRQYCGHSFPAWNMALNGTLEFKGFDLMFQ
                     FTSSFGKDFKLFNGPRSGYDRFDDNSNYRADYDGYDAVTNPNGVDPRPLYADARNARG
                     DQDRWLENGSYLRLKQVGIGYTFPKKAFNNVLSNFRLFVNAQNLFTITKYRGLDPEFL
                     NSNIWDRGYDSAPFPNPYGITVGTQISF"
     gene            3453..5030
                     /locus_tag="PLM04_03950"
     CDS             3453..5030
                     /locus_tag="PLM04_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HON02265.1"
                     /translation="MKKYLYLLMIASTFLLNGCDVDVINPNTLTQATFWKTQRDAEYG
                     VNAIYNMFYKPGTYSRWIWFRLDLTSDEGWSQSPWAELKEWTRFQYVNYNFWEGNAWT
                     YRDCYEAIFRANQALENIPNIEMDETVKNQLLGQAYFLRGLYYYDLALLWGSTNNSLP
                     INLKPSKPADTPEGHTGEAVYNQAISDLTQAVNLLPEQWDSNNLGRATKGAAYALRAK
                     CYMQLHDWTKAKNDLYWLVEGDGKQYYDLVANYEDNFTKDTENNKESVFEIQYSDVHK
                     APAGDGDFDVDPNLGLNRGQFFAPPGIGWTDGELRPWIVEEFKKERDLNGNFDIRLKY
                     TAFYEGMNQDFTDNNRIYRYRLDGADANIWNLQNWKGRVFFRKYGSDYYRDYDDYHNP
                     TNVRLIRYADVLLMYAECIANTENDFSKAVDLVDRVRQRANMPKLSVNHLDATQSKEK
                     FLKRLQTERALELCSEGHRWADIKRWGLVDTQNGLNELIARDADFQNFVIGKHAVLPI
                     PSDDVNNNPNQQQNPNY"
     gene            5032..5994
                     /locus_tag="PLM04_03955"
     CDS             5032..5994
                     /locus_tag="PLM04_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HON02266.1"
                     /translation="MKLKYVVILLSLIFLSCRKGKIEDQETNKILVPMADPYILLYDG
                     MYYAYGTGADNGIEVMTSSDLKTWKRRANTTSGLALYKDDSYGEKWFWAPEVYYVNEK
                     FYMYYSADEHICVAISDSPLGPFRQVEQKPMLEEEKAIDNSLFVDDNGKAYLFFVRFN
                     DGNNVWVAELEDDLMTLKKETMHHCISVSQPWEEIWPRVNEGPFVIKHNGIYYMTYSA
                     NSYESPFYGIGCATATNIMGDWTKYPDNPLLQKPGNLVGVGHNSLFFDKEGNLRIVFH
                     AHHDINTIHPRNMYISNVGFKHVDGIDKMFINPNYLTPFLEVIR"
     gene            6292..8460
                     /locus_tag="PLM04_03960"
     CDS             6292..8460
                     /locus_tag="PLM04_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HON02267.1"
                     /translation="MKRFRKFLFLSVFLFTSWLLIAQPVKTVNGIIIPSEGKYIEMSV
                     VNNSVFCLSFNSSGNSAEIKSAFIAEKDGNTKNHFSIVSKGSVVGLKTGFGKLLFDTQ
                     KQTWTLSDNKGKVLFSNATLSWGDSVQKISYTLPQKSLFYGSGNYSTKNLLKSQSAGK
                     QGNGTADIPYCWTTVGFCTFGTTGNDNHPATWKAVENSSVDWTFSGKTGNLYIWPASN
                     LYEATNNLIAITGKAKIPPRWAFGFLQSQWGWADRAYIENAIDNFRSRKLPVDAFIYD
                     FEWYTTFPDYFVKENGIKGFSDFSFNEKLFPEPQKQIAEYKKNGIKFIGIRKPRLGDS
                     LKLIEARKNGWILKSDYNNRDLDFSNPGLVKWYKSQTRPLLEAGVDAWWNDEGESYYT
                     CYYYWNKTESELLNEVFPNARFFSINRSFSPGNQRLGYCTWNGDIRSTWKALQETPAD
                     LLNFSLSGMFYSTCDIGGFGGKDPSKELVVRWYEAGVFLPVMRAHSNLFTKPRFPWLW
                     GDDAEAAIRKSLNLRYQLIPYFYSVAHEAYQKNKPIMRPLIMEFPNDTALLNLTDEWL
                     VGKDLLAAPILNEGGNRSVYLPDDKWYDFQTNQLITGPCKLAVSKALDEIPVYVRAGA
                     ILPIGPIVQHTEQDTTSVIEVHIYPGKNGSFCLTEDDGKSYDYVSGKVRNTVFEWNDA
                     TKTLSWKATGNFAGKYKSIKAVLFEVAKTASLSERGKIKF"
     gene            8562..12560
                     /locus_tag="PLM04_03965"
     CDS             8562..12560
                     /locus_tag="PLM04_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HON02268.1"
                     /translation="MYKFRAYLILLLLLLFVQSGRGLPSYQFKHFDINDGLSQNTVLS
                     IFQDKQGFMWFGTKNGLNRFDGSNFKVFKFFPNNEVKDNVFRCILQDKDEKLWLGTDE
                     GVYIYEPRSEKFQRFVAKTTANDSVRGVVSDMIIDSDGDIWISVEEKGVYLYRYESNK
                     LDHFPVKTPPAGLKGIKLCENKGKGVWAFPYSMPFLQIDKKNRKIQPFSLNQGNEILY
                     NTGEIGSVIEEQNNLLVGTSLQGLLQINLVERTYNKLLASDETGQPIFVRNILRKGDD
                     LWVATESGIYIYNLYSRDVINLRHNNVIPYSLSDNAVYSLYEDNEGGVWMGTYFGGVN
                     YFPNRLTYFEIFYPLENENSIKGRRVREFCENTDGKLWIGTEDNGLNLFDPETSKFLP
                     VDSKLNSLYHNIHALFRDGNTLWIGTFSKGLNRYDLKTKTLKIYYKTSQPNSLSENSV
                     FSICKDRQNTLWIGTLSGLNIFNYQNDSFTRIKELEGAFVQDIFEDTDGNIWVATFVK
                     GLFLFNPKTHTWKNFVHTSNAGSLPYNKTTSVFEDSKRRIWITTEGGGFCRYDKKNQS
                     FVTVNSTNGLPNDVVYQIQEDNSGNLWLSTNSGLVKYVPETGIMENFTVNDGLKTNQF
                     NYKSSYKAPDGTLYFGSIDGFVRFNPAYFKENNLLPPIVLTDFYLNNSPANVSDADSP
                     LKENISFAEKIRLPYNKNSFTIKYAILNYSGLRPKNVVYKLEGFDRQWIPANGSQSVV
                     YSNLNPGKYRLMISFDTNSSGGMNKVSKTLDIVIKPPFWETWWAYLLYFLITIAGIFE
                     LINYFRQRTKRIHLEKMRDFEQEKEKELYESKIDFFTNVAHEIRTPLSLIKAPLDQIL
                     RNDETVTPDVKDNLKVMSKNTDRLLDLTNQLLDFRKTEAELYKLKEKRTNLSELMKET
                     FDRFTPFAKQKNISFHLNLPENEVFTKIDAEAFTKIISNLINNAVKYCDSMVNISLEV
                     VAENEIEKLVFKTENDGAIVPERFKNEIFKPFTRLNKEKDKIVTGTGIGLALTKSLTE
                     LLKGDITYKVENGLNVFRVTLPFNPLTGNEWIEEKTEMQETPSRKEEDKVEKSLKRPL
                     ILLVEDDKGMMEFLTKCLKREYSVLSASDGKEALEVLKNKNIDLVVSDVMMPEMDGFE
                     LTSYIKSHIEYNHIPVILLTAKVNVQSKVQGFEVGADAYIEKPFSVDVLLAQIASLLQ
                     NREKFREAFLKNPFKGLNHVELNKSDEEFLQKLDDIIQENISNPDFNIEDLSEHFFMS
                     RASFYRKIKGILDLTPNEYLRVARLKKAAQLLKEGNQRITEICYLVGFNSPSYFAKCF
                     QQQFGVLPKDFS"
     gene            12668..14479
                     /locus_tag="PLM04_03970"
     CDS             12668..14479
                     /locus_tag="PLM04_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HON02269.1"
                     /translation="MLRIKLLLLSSLFSVITFAQWQPAGEKIKTRWSSQIDVNNVLPE
                     YPRPLMERSEWMNLNGLWNYAILPVGKPAPSAFDGKILVPFAVESSLSGVQKNVGKAN
                     ELWYEREFTIPSKWKNNRVLLHFGAVDWKTDVWVNDVKVGRHTGGYTPFSFEITTALK
                     NGSNKLIVKVWDPTNDGYQPRGKQVNKPEGIWYTPVTGIWQTVWLEPVPDKFITQVKT
                     TPNIDINILNIEVATNDFSASDRIEVKVMDGSKVVATAQSINHLPVEIVMPDQVKLWS
                     PESPFLYDLEITLWNGTKQIDKVKSYAAMRKYSIKRDENGIVRLQLNNKNLFQFGPLD
                     QGWWPDGLYTAPTDEALKFDIQKTKDFGFNMIRKHVKVEPARWYTYCDRLGMIVWQDM
                     PSGDGGPQWQPHQYFSGLEWLRSPESEANFRKEWKEIIDFLYSNPCIGVWVPFNEAWG
                     QFKTKEIVEWTKEYDPSRLVNPASGGNHYPVGDMLDLHNYPNPALYLYDAQRATVLGE
                     YGGIGWAVKGHLWEPDRNWGYVQFNSAKEVTDEYVKYAEQLMNLILHGFSAAVYTQTT
                     DVEVEVNGLMTYDREIIKMDESRVKQINQKICNILKN"
     gene            14458..15621
                     /locus_tag="PLM04_03975"
     CDS             14458..15621
                     /locus_tag="PLM04_03975"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HON02270.1"
                     /translation="MQYFKELAICISVLLCFSCQKNNEIAAKSPSLSIRWDESSLVCI
                     AAEGGYPRVKRLNDGSLLAAYENRKGDVLVKKSRDEGKTWSDSVLVFGSFDYIHPSNG
                     LSAKINIANPEFIQLGNNDILFACNFRPNKDEVYPFSIAVKRSRDNGKSWSEQQIVYQ
                     AAPRFIDGCWEPSFLILPDGRIQLYFANEFPYQQSNEQEISMLESNDNGTSWSADKKT
                     VSFRKGFRDGMPVATTDGNEIFVAIEDNGFDQFKPFIVKNAIADNWKEPVLAESPNRY
                     SALKEPLPSSVYAGAPYLIHTKEGYYVLSYQTTHHRTSNWELSTMEVVVSDKPSDFVS
                     PSQPFAVPLSKEAKWNSLCDLGNGEIAAVSSTNFKSDKIGIWMIKGKIVDNRK"
     gene            15628..16632
                     /locus_tag="PLM04_03980"
     CDS             15628..16632
                     /locus_tag="PLM04_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HON02271.1"
                     /translation="MKRSLGIILLTLFFISCKGSEAIIPETENVSTYKNPIIPRSLPD
                     PTVMKASDGNFYLYATEDIRNVPIFRSSDLVNWNFLGTAFTDQTRPTFVSGGGIWAPD
                     INYVNGKYVLYYSMSVWGGEWSCGIGVATADHPEGPFTDHGKLFISDEIGVQNSIDPC
                     YFEENGKKYLFWGSFRGIYGIELSDDGLSVKPGAQKVQIAGTAYEGTYIHKRNGFYYL
                     FASIGSCCEGLNSTYTAVVGRSDNLFGPYLDKSGQSMLENHHEILIQKNSKFVGTGHN
                     SEIVSDDKGQDWIFYHAVWVDYPQGRVLMLDKVQWMNDWPYVTDKCPSLEAEKPYFNE
                     "
     gene            16682..17752
                     /locus_tag="PLM04_03985"
                     /pseudo
     CDS             16682..17752
                     /locus_tag="PLM04_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
     gene            17771..18844
                     /locus_tag="PLM04_03990"
     CDS             17771..18844
                     /locus_tag="PLM04_03990"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HON02272.1"
                     /translation="MKPENFNEKIDGKQISLFTLKNRQGMTAQITNYGGRVVSLWVPD
                     KKYDFKDVITGYSSLKGFQQSTEVYFGALIGRYGNRIAKGRFELNGKTYQLPINDGEN
                     HLHGGPKGFHTVIWDARPFKNANNEDALELKYFSRDGEEGYPGNLTVKVVYTLTNDNE
                     LKIEYSATTDKPTIINLTNHMFFNLHGFSDGIAKSINSHVLEINASNYTPTDKGLIPT
                     GIIAPVKGTPMDFTVPTPIGERVNENFIDLINGKGYDHNWVLNKKGDKLTEAAVVHEP
                     SNGIQMRVITDQPGVQFYGGNFLNGTERGKYGEVYTYRTSFAMETQHFPDSPNHPNFP
                     STVLNPGQQYQHVCIYKFEVGGN"
     gene            complement(19102..21528)
                     /locus_tag="PLM04_03995"
     CDS             complement(19102..21528)
                     /locus_tag="PLM04_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02273.1"
                     /translation="MRQFVFTAILLFGTLMSFGQHWIKGKVLDENGHPLVGADIYLQK
                     EGIGKTTDKNGTFLFENLKNKNYFLQVSYLGYETQWVETTPDKEITIQMNRISIEIDE
                     ITVTSLRANERSPIAYSEIKKEEIESRNLGQDIPYLLALTPSFIVTSDAGNGVGYTGF
                     RIRGTDANRTNVTINGIPLNDAESHGTFFVDFPDLAASLSSVQVQRGVGTSTNGAAAF
                     GASINMQTESVSLEPYADLSTSLGSFATNKNSIKVGSGMLDNNFAFDARLSNVTSDGY
                     IDRASVDMQSYYFSGGYFSDKTVLRLLTFGGKEKTYQAWNGVDAEIMKTNRRYNDLGA
                     YTDNNGELKFYDNQTDNYLQTHYHLYWLQKWNSHLHSNVATHLTRGIGYYEEYKIDKK
                     YAEYGLTPAIIGFDTLKTTDLVRQKWLDNYFYGITGALNYEKKNTNFSLGGAVNRYDG
                     EHYGKIIWVRNAANLDIGKDWYRSSSIKDDANIYAKLNTETFKNLFASIDLQYRYINY
                     RMDGTDDKYDKINQSMRDITQQHIYHFFNPKFGFTWQPSTRNEFYASYSVSHREPNRN
                     NFTEAGANERPTNETLYDTEAGYRFRSSRFSAGTNFYYMKYKNQLILTGKVSEIGEFL
                     TTNIPDSYRAGIELTAGWKITNFLNWDGNCTFSHNKIKNFTEQDIDVYDENWNYIGTR
                     NNELGTTDIAYSPNVIANSIFSFHLKPFEAAFHSNYVSRQYIDNTSDIQRSIDPYFVQ
                     NLNLKYSLAISPIKTLEFLVQINNLFNEKYETNGYTWYSCYLGNERHNELRYFPQAGT
                     NFLASARLKF"
     regulatory      complement(21580..21675)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(22104..22874)
                     /gene="trpA"
                     /locus_tag="PLM04_04000"
     CDS             complement(22104..22874)
                     /gene="trpA"
                     /locus_tag="PLM04_04000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HON02274.1"
                     /translation="MNRIQQLFTQKKENILSVYFTAGFPKPNDTVPILESLQKYSVDM
                     AEIGIPFSDPLADGPVIQNSSQIALKNGMNLKKLFSQLANIRASVHIPLVMMGYLNPI
                     MQFGFENFCSECKKVGVDGMIIPDLPMDVYLTEYREIVEKNGLDFIFLITPETSEERI
                     RQIDEHTHGFIYMVSSAGVTGTQNSFDHHIDYFNRINSMHLKNPRLIGFGISNKLTRE
                     TASRYSSGVIIGSAFIKALQESSDVESAVKLLIKKLED"
     gene            complement(22901..23584)
                     /locus_tag="PLM04_04005"
     CDS             complement(22901..23584)
                     /locus_tag="PLM04_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HON02275.1"
                     /translation="MRSVEKNNIVPSRFADTTKKKLIKVCGMKFPENIRAVARLRPDF
                     MGFIFYPKSPRYAEPLNVSVLNELPPEIGKIGVFVNEKLDQILATVYKYHLDGVQLHG
                     SEMVKMCNQLHDTGLLVIKAFPVAEAYNFKVMKRYEKSCDYFLFDAQTPIYGGAGQKF
                     DWQLLENYKEKTPFLLSGGISIEDLEDINKIQHPAFAGVDLNSRFEISPGLKNVELLS
                     NFIDRYRSL"
     gene            complement(23736..24563)
                     /gene="trpC"
                     /locus_tag="PLM04_04010"
     CDS             complement(23736..24563)
                     /gene="trpC"
                     /locus_tag="PLM04_04010"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HON02276.1"
                     /translation="MTDSTLKNNHLPNPGENFLNNILLNKIKEVNKRKLNVSITELEN
                     SNFFDRSTISLKQKLQNSSLGIIAEFKRKSPSKGWIHADANVQEVTIGYNVEGASGIS
                     ILTDTEFFGGNIEDVTTVRNKISCPILRKDFVVDEFQIYEAKAIGADVILLIASALTI
                     YEVKNFSRLAHQLGLEVLLEVHNKEELSYLNENVDMVGVNNRNLKTFQVDTGISANLA
                     AFIPPEFVKISESGISNVETVKELNRLGYSGFLMGENFMKEVHPALALKEFLRQLNE"
     gene            complement(24573..25571)
                     /gene="trpD"
                     /locus_tag="PLM04_04015"
     CDS             complement(24573..25571)
                     /gene="trpD"
                     /locus_tag="PLM04_04015"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HON02277.1"
                     /translation="MKQILNRLFNHQSLTREESAEVMRNIGAGMYNESQIAAFISVYL
                     MRSIELDELIGFRDALMSMAIPMDLSDFDPIDIVGTGGDEKNTFNISTCSCFVLAGAG
                     YKVAKHGNYGATSISGSSNVLEYYGAKFTTDTDVVRRSLEESGFAYLHAPFCNPAMKN
                     VAPVRKNIGVRTFFNVLGPLISPIRPRYQCLGVYNLKMMRLYNYIYQRLGTNYTIVYT
                     LDGYDEVSLTDDCKIINNFGEYVYSPQQLGFQRVKQEELWGGTTVEHAAEIFMNVLDN
                     KASDAQRNVVVINSAFAIQTRCPEKSLEECKAEAIASLACGKAKKAFVKFEEIYHL"
     gene            complement(25650..26231)
                     /locus_tag="PLM04_04020"
     CDS             complement(25650..26231)
                     /locus_tag="PLM04_04020"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HON02278.1"
                     /translation="MKLLVFDNYDSFTYNLVHIVKKLGFTDVEVHRNDRISLEEINRF
                     DKIILSPGPGVPSESGILLELIKTYASSKSIFGVCLGVQAIAEAFGGSLLNLPQVYHG
                     IASEVSIFDDEVLFRNLPEKIIAGRYHSWVVNDSTLPPCLQVTAKDEQGTIMALKHRT
                     LDVRGVQFHPESVLTPSGEQILKNWLEFPLSGK"
     gene            complement(26317..>27702)
                     /locus_tag="PLM04_04025"
     CDS             complement(26317..>27702)
                     /locus_tag="PLM04_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HON02279.1"
                     /translation="ETPVGIYLKIRDLYTHSVLLESSDYHAAENSYSFIAFSPIGGIS
                     VNHFLVHEYYPDGTKIQTPVTDKMTVADRFDIFLKSFERIEQDNEVPAVVNGLFGYTS
                     YEAVQYFEDIRLSARETVPGSMPGLHYVLFKYIIAVNHFKNELTIIENLLENEKSAMN
                     EIEEILLNNNIAAYEFSGIGSEKCDINDEDYRRMVKRGKEECFKGNVFQIVLSRRFYQ
                     QYQGDDFMLYRTLRSINPSPYLFYFDFADFRIFGSSPEAHLVVDAKAQKAYIKPIAGT
                     FRRTGDDIKDLELAEKLKNDEKENAEHVMLVDLARNDLSRNADEVKVETFREVQYYSH
                     VIHLVSCVSGKLRPGTKIMKFFADTFPAGTLSGAPKIKAMQLIDQIEPHDRGPYGGCI
                     GYIGFDGGFNQAITIRSFISKNNTLFYQAGAGIVAKSDEENELQEVNNKLAALKQAII
                     LAGKRNNYQTE"
     assembly_gap    27703..27802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27803..28908)
                     /gene="trpB"
                     /locus_tag="PLM04_04030"
     CDS             complement(<27803..28908)
                     /gene="trpB"
                     /locus_tag="PLM04_04030"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HON02280.1"
                     /translation="MPENSQKSRNGADRKLYQPDEKGFYGEFGGAYIPEILYASVEQL
                     KKAFLSIKGDENFKNEYYDLLKNYVGRPTPLYFARRLSELYGVNIYLKREDLNHTGAH
                     KINNALGQVLLARKMGKTHMLAETGAGQHGVAAATACALMGMDCTVFMGKTDIERQYL
                     NVQKMRMLGARVEPVTSGNMTLKDATNEAIRYWCSHPDSYYIIGSTVGPDPYPEMVGY
                     FQSVISEEIKWQLQEKIGRNYPDYLMACIGGGSNAMGTYFHFLDDERVNIISAEAGGM
                     GIETGKTAATIHLGKTGIIHGAKTLILQNEDGQIDEPYSVSAGLDYPGVGPAHAFFTK
                     IGRTKVYAINDDEAMEAAFELTRLEGIIPAIESA"
BASE COUNT         9023 a         5302 c         5942 g         8889 t
ORIGIN      
        1 caaatcttta accgagaaaa aagttttaaa agaaaatgag tagcaataaa acacagcgag
       61 ttacaaactt gaatatatac tttacacttt acaaacaaag agataacatc atcaggtcaa
      121 attcagctct tttgaagaca agaaaattta tgcattagca aataatttaa gaaagagcaa
      181 tacatcggga atttaggata tgagtattaa attgttgagt agaaatcatt ctttaaaaaa
      241 gagaaaattg cataaccttc gaattttgat acctgtagct ggcaaactaa taagtttaaa
      301 aaatcgaata attgataata tttttttatt ttttgataca aaaattctat gttgaattta
      361 ttttagtatc tacttttatg gagcaaattt ttgtgaaatt tatcaattta aaaaattaaa
      421 tctatgaaaa acaaatttta tttattgcta gttcttttat cctttccgct tttaatattt
      481 gctcaagcgc aaagagaagt caaaggaaaa attttgaacg aaaatggaga acctgtcata
      541 ggggccaata tttttgaatc gggtaatcca acgaatggga ctatttctga tttggaaggc
      601 aattttgtaa taaagatatc tccgcaatcg gctattgaga tatcgtatat tggttatgtg
      661 ccacaaaaaa ttaaagatcc aaaatcacct cttaccattc agttagaagt ggaccaacgt
      721 tctttggatg aagtcgtagt cataggttat cagaccgtga aaaaatcgga tttgaccggt
      781 gctgtttcag tttttaagcc cgaaacaatg aaaaacacgg ttgttaccgg tacaatcggt
      841 gaagctttat acagcgttcc gggtattttt gttcgatcag ccggtgctcc aggaaatgaa
      901 ggttttattc aaatcagggg aacttcaact tttggcacaa gtaatccttt atatattata
      961 gatggaatcg atgttggaac agctaaccga gacttcaact ataatgatat cgaaagcatt
     1021 caagtattga aagatgcttc agcagctgct atttatgggt caagggcagc taatggagtt
     1081 attattatta ccaccaagaa gggtagcaag ggaaagatga agatcgattt ttcggcaaaa
     1141 aacacttttc aatggttacc tcagtataat ttaaccaatc gtgaacagtg gataactttg
     1201 aatgatcttg ctttcaagaa tgccggaagg cagccagcca accatagcga tggtaatacc
     1261 aattggcagg atgaagtttt caaaaccggt attttacagg attatgattt gagtttttcg
     1321 ggtggtgggg aaaccaatac ctattactta tctgccaact accaaaccaa tagtggaact
     1381 acttacggaa ccaacagtga aagaataact gcccgtataa atacttcttc ttttcgcgac
     1441 ttcgggaaga atgtaagatt tacgattggt gaaaatttaa ttatcagcaa ttactttgta
     1501 gatgaattga ataccaaccc gattacagat gtttggagaa tgttgccaac cataccggtt
     1561 tatgatccat ctaatcccgg agggttcggt tatggcgatg ggtccaaaga tgttactttt
     1621 ggtactaatc caattgctcg tgaaaatttg gaaaatacta ccaacgaaaa tttaagaatc
     1681 agaggtaacg catttgctga attaagtttc tttaaatcat tgaaatataa gtttaatctt
     1741 ggcttggtta ccagttcaga ttctcacatg tatttacgca aagttggtaa ttggacgtat
     1801 aatcaaccaa tagatccttc tagtttgaac agaaataaag ctttatacaa atttatggtg
     1861 tacgataaca cactggaatt taataagact ttcggcgtaa ataacattgc tgctgtagca
     1921 ggtacaagtt tttccactga agattattct caaatttggg gaacaaaaaa taatgtgcta
     1981 acttcaggag atgactattt cacacaattg gatgctgctt tgttggatcc aaaaacagga
     2041 agttatcaaa atttggcaaa attattctca atttttggac gattaaacta tagttatgat
     2101 gataaataca tcctcagtgc aacggttcgt cgggatgcct cttcaaagtt tggtccaaat
     2161 tacagaaatg gagttttccc atcaatagcc ggagcatgga gaattagtaa ggaaaaattt
     2221 ttcaatgtgt catggattga tgatttaaaa atcagagcaa attatggtat tttgggaagt
     2281 tccaatatag gttattggga ttgggttcca tttatcaata tatttcctca ggcaattttt
     2341 ggtgcaggcc aaaatattaa tacaggtatg actcaggtta aattggtcaa tactgatttg
     2401 aaatgggaag aactacatca aacaaatgca ggattcgatg cttatttgtt gaatcgtcgt
     2461 ttggaagttg tgggtgatta ttttaacaaa accactcagg atgttttgac tcccatgcag
     2521 atattgttga caacgggtaa taatggaggt aatccaatgg taaatgccgc cactttaaaa
     2581 aataccggtt ttgaagtttc ggttaattgg agagatcgca taaatgattt aaattattct
     2641 gttggtttga gtacttcata cttaaaaaat gagattgtag aacttggata tggtcgtgaa
     2701 agctttacac aatgggatac caaatctttt gtcgggaaac cgattggtga ttggtatctt
     2761 atcaaaacgg acggaatttt cagatctaca gatgaagtgt tgaaccatat taatagtaag
     2821 ggacaagtga ttcagccgtc tgctaagccc ggagatattc gttttgttga tttcaatgat
     2881 gatggtcaga ttacggatgc cgaccgtcaa tactgcggac attcgttccc agcttggaat
     2941 atggctttaa acggaacgct cgaattcaaa ggttttgatt taatgtttca gtttaccagt
     3001 tctttcggaa aagatttcaa actgttcaat ggaccaagaa gtggttatga caggtttgat
     3061 gacaattcaa attacagagc cgattatgat ggttatgatg cggttaccaa tccaaacggg
     3121 gtggacccac gacctcttta cgccgatgcc agaaatgctc gtggtgatca ggatcggtgg
     3181 ttggaaaatg gcagctatct acgcttgaaa caagtcggaa ttggatatac ttttccaaaa
     3241 aaggctttca ataatgttct tagtaatttt agattgtttg tcaatgctca aaatcttttc
     3301 accattacca aatatcgcgg gttagaccca gaatttctca attccaatat ctgggacaga
     3361 ggttatgata gtgcaccttt cccaaatcct tatggaataa cagttggaac acaaatttct
     3421 ttttaataat tgaccattta aattagaaca caatgaaaaa atatctttat ttattgatga
     3481 tagcatcaac tttcctttta aatggatgtg atgtagatgt tataaatccc aatacactta
     3541 ctcaggcgac attttggaaa acgcaacgtg atgcggaata tggtgtcaat gcaatttata
     3601 acatgtttta taaaccaggc acttacagcc gttggatatg gttccgactg gatttgactt
     3661 ccgacgaagg atggagtcaa agtccctggg cagagcttaa agaatggaca cgttttcaat
     3721 atgtaaatta caatttctgg gaggggaatg catggactta cagagattgc tacgaagcaa
     3781 ttttcagagc taatcaggct ttggaaaata tcccgaatat tgaaatggat gaaacagtta
     3841 aaaatcagct tttaggacag gcttattttt taagaggttt gtattattat gatttggctt
     3901 tactttgggg aagtaccaac aatagtcttc cgattaactt aaaaccatcc aaacctgctg
     3961 atactcctga gggacataca ggagaagctg tttacaatca ggcaatcagc gatttaacac
     4021 aggcggtaaa tttattgccg gaacaatggg acagcaataa tttgggacgt gctacaaaag
     4081 gagctgctta tgccttaaga gcaaaatgtt atatgcaatt gcatgattgg acaaaagcaa
     4141 aaaatgattt atactggttg gttgaaggag acggcaaaca atattatgat ttggtagcta
     4201 attatgaaga caactttaca aaggataccg aaaacaataa agaatccgtg tttgaaatac
     4261 aatactccga cgttcacaaa gcccctgccg gtgatggtga ttttgatgtc gatcctaatt
     4321 taggattgaa tcgtggacag ttttttgctc ctcccggaat tggatggaca gacggagaat
     4381 tacggccatg gatagtggaa gaatttaaga aagaaagaga tttgaatggg aacttcgaca
     4441 ttcgtttaaa atacacagct ttctatgaag gtatgaatca agattttacc gataacaaca
     4501 gaatttatcg ttataggctt gatggtgctg atgctaatat ttggaatttg caaaactgga
     4561 aaggacgagt attttttaga aaatatggtt ctgattatta tcgcgattat gatgattatc
     4621 acaatccgac caatgtaagg ttgattcgtt atgccgatgt attgcttatg tatgccgaat
     4681 gtatagccaa tactgaaaat gatttttcaa aagctgttga cttggttgac agggttcgtc
     4741 aaagagcaaa tatgcctaaa ctgtctgtta atcatcttga cgcgacacaa tcaaaagaaa
     4801 aatttttgaa aagattacaa accgagcgtg ctttagaatt gtgttcagaa ggtcatcgtt
     4861 gggcagatat aaaacgttgg ggattggtag atactcagaa tggtttaaac gagctgattg
     4921 cgcgtgatgc agatttccaa aattttgtta ttggaaaaca tgccgtgttg ccaattcctt
     4981 cggatgatgt gaacaacaat cctaatcaac aacaaaatcc caattattaa aatgaagtta
     5041 aagtatgttg ttatattgtt gagtttgatt tttctgtcgt gcagaaaagg gaaaattgaa
     5101 gatcaggaaa caaataagat cctggttcct atggccgatc cgtatatttt gctttacgat
     5161 ggaatgtatt atgcatatgg taccggagca gacaatggta ttgaagtgat gacttcatcc
     5221 gacctcaaga catggaaacg gagagcaaat acaacaagtg gacttgcact ttacaaggat
     5281 gattcctacg gcgagaaatg gttttgggca ccggaagtgt attatgtgaa tgaaaaattt
     5341 tacatgtact attcggctga tgaacatatc tgcgttgcca tctccgattc tcctttgggc
     5401 ccatttcgac aagtcgaaca gaaacccatg cttgaagagg agaaagccat tgacaattcc
     5461 ttatttgtgg atgataatgg aaaagcctac ttgttttttg ttcgcttcaa tgatggaaac
     5521 aatgtttggg tggctgaatt ggaagatgat ttgatgactt tgaaaaagga aaccatgcat
     5581 cattgcatca gtgtttcaca accgtgggaa gaaatttggc cacgagtaaa cgaaggtccg
     5641 tttgttatca aacacaatgg tatctattac atgacttatt cggccaatag ttacgaaagc
     5701 cctttttatg gaattggttg tgctactgca acaaatatta tgggtgattg gaccaagtac
     5761 cccgataatc ctttgttgca aaagccggga aatttggttg gtgttggaca caattcgctt
     5821 tttttcgaca aagaaggcaa cttgagaatt gtatttcatg ctcatcacga tataaatact
     5881 atccatccgc gaaacatgta tatcagcaat gtaggtttta agcatgtgga cgggatagat
     5941 aaaatgttta ttaatccaaa ttacctcact ccatttttag aagtaattcg ctaattttga
     6001 tttgaattcc ataaatgcgt tggaggattt gctcgattta ttcgtcatta aattgctcca
     6061 acgctttttt atatgacttt cgaatagaaa gtcagaaaaa ataagttgct aaacaatgta
     6121 aatcaatttt ctctctttcg aaaacaatct atttagtgtg aatatttatt tttaaatttg
     6181 tctattctta ttcgttttga gctgaggtgc taaagatttt tctatcgttg ggataataaa
     6241 tctttgattt gcatccattt gccgataaaa accaaactaa aacataacca catgaaaaga
     6301 tttcgtaaat ttttgttttt gagcgtattt ttatttacaa gttggctact tattgctcag
     6361 cctgtaaaaa ctgtcaatgg aataattatt ccttctgaag gaaaatacat tgaaatgtca
     6421 gttgttaaca attcggtttt ttgcttgagt tttaattctt cgggtaattc tgccgaaata
     6481 aaaagtgctt ttatcgcaga aaaggatgga aatacaaaaa atcatttttc aattgtttcc
     6541 aaaggatcgg ttgtcggact taaaaccgga ttcggaaaac tattgtttga cactcaaaaa
     6601 caaacatgga ctttgtcgga taacaaagga aaagttttgt tttcgaatgc aaccctgtca
     6661 tggggagatt ccgttcagaa aatcagttat acgcttcctc aaaagagctt attttatggc
     6721 tccggtaatt attctacaaa gaatttatta aaatcgcaat cggctggaaa acagggcaac
     6781 ggaacggctg atattcccta ttgttggact acagtcggtt tttgcacttt tggaaccacc
     6841 ggaaacgata atcatcctgc aacttggaaa gctgttgaaa attcctctgt agattggact
     6901 ttctcaggaa aaactggaaa tttatatatt tggccggctt ccaatcttta cgaagccact
     6961 aataacctga ttgctattac cgggaaagct aaaattcctc cccgctgggc ttttggcttt
     7021 ttgcaatcgc aatggggatg ggccgatcgt gcatacatcg agaatgccat cgataatttt
     7081 cgttcgcgta aacttccggt cgatgccttt atttacgatt ttgaatggta caccactttc
     7141 cccgattatt ttgtgaaaga aaatggtata aaagggtttt cagatttttc ttttaatgaa
     7201 aaattatttc ccgagccgca aaagcaaatt gcagaatata aaaagaatgg aataaaattt
     7261 ataggtatcc gtaaacctcg tcttggtgat tcgcttaaat taattgaagc gcggaaaaac
     7321 ggttggattt taaagtccga ttacaacaat cgtgatttgg attttagtaa tcccggtttg
     7381 gtaaagtggt acaaaagtca aactcgaccg ttactggaag caggtgtaga tgcatggtgg
     7441 aacgatgaag gagaatcgta ttacacttgc tattattatt ggaacaaaac agaatcggaa
     7501 ttactgaatg aagtttttcc caatgctcga tttttctcga ttaaccgatc attctcaccc
     7561 gggaatcaac ggttgggcta ttgtacgtgg aatggagata ttcgttcaac ttggaaagct
     7621 ttgcaggaaa cgccggccga tttattaaac tttagccttt cgggcatgtt ttacagtact
     7681 tgtgatattg gaggttttgg aggaaaagat ccgtcgaaag aattagtagt tcgatggtac
     7741 gaagccggag tttttttacc tgtaatgcga gctcattcca atttgtttac caaaccacgt
     7801 tttccttggt tgtggggtga tgatgccgaa gctgccattc gcaaatcgct taacttgcga
     7861 taccaactta ttccatattt ttacagtgtt gctcacgaag catatcaaaa gaataaacct
     7921 attatgcgac cgttaatcat ggaattcccg aacgatactg cacttttgaa tttgacagac
     7981 gaatggcttg tgggtaaaga tttactcgct gctccgattt taaacgaagg cggaaatcga
     8041 agtgtgtatc ttccagacga taaatggtac gactttcaaa ccaatcaatt gattaccggt
     8101 ccttgtaaat tggcagtttc caaagctctc gacgaaattc cggtatatgt aagagccgga
     8161 gccattcttc ctattggccc aatcgtgcag cataccgaac aagacaccac ctcggtcatt
     8221 gaagttcata tttatcccgg gaaaaatggt tcattctgct taacggaaga tgacggaaaa
     8281 tcatacgact atgtttccgg caaagtcagg aatactgttt ttgaatggaa cgacgctaca
     8341 aaaacactca gttggaaagc taccggcaat tttgctggaa aatacaaaag tataaaagcc
     8401 gttttgttcg aggtcgctaa aactgcttcg ttaagtgaaa ggggaaaaat taagttttaa
     8461 tctttgggaa taaagaaaat tatttcaaat atggttttat cttctaaaga ttttcgctga
     8521 agtgattcaa tgtgttccaa tgccaacatt taaaatttca catgtataaa tttcgtgcat
     8581 atctgattct ccttttacta ttgttgtttg ttcaatctgg taggggcctg ccttcctatc
     8641 agttcaaaca ttttgacatc aacgatggtt tgtcgcaaaa cacggttttg tcgatttttc
     8701 aagataagca gggattcatg tggtttggca caaaaaacgg gttgaatcgg tttgatggtt
     8761 caaactttaa ggttttcaag tttttcccaa acaatgaagt aaaggacaat gtttttcgct
     8821 gtattttgca ggataaggac gaaaaattat ggttgggaac cgacgaagga gtttacattt
     8881 acgaaccaag aagtgaaaag tttcaacgtt tcgtagcgaa aacgaccgca aatgattcag
     8941 taagaggtgt cgtcagcgac atgattatcg acagtgatgg agatatctgg atttcggtcg
     9001 aagaaaaggg agtttatttg tatcgatatg agagcaacaa attggatcat ttcccggtta
     9061 aaacgcctcc cgcaggattg aaaggaatta aactctgcga aaacaaagga aaaggagttt
     9121 gggcttttcc ttattccatg ccgtttctgc aaattgacaa aaaaaaccgt aagatccaac
     9181 cgttttcttt gaatcagggc aatgaaattc tttataatac gggtgaaatc ggaagcgtaa
     9241 ttgaagaaca aaacaatttg ctggtaggta cttccttgca gggactttta caaattaatc
     9301 tggtagaacg aacttacaac aaattattgg cttcggacga aaccggacag ccgatttttg
     9361 tgcgaaatat cttgcgaaaa ggggatgatt tgtgggtggc aacggaatcg ggaatttata
     9421 tttacaatct ttattctcgt gatgtcatta atttaaggca caataacgtt attccttatt
     9481 cgctttcgga caatgctgtc tattcccttt atgaagataa tgaaggcggc gtatggatgg
     9541 gaacttattt tggcggagta aattattttc cgaaccgact gacttatttc gaaatttttt
     9601 atcctcttga aaatgaaaat tcaataaagg gaagaagggt tagagagttt tgtgaaaata
     9661 cagatggaaa attgtggatt ggaacggagg acaacggatt gaatttgttc gatccagaaa
     9721 cttcaaaatt ccttcccgtc gacagtaaat taaactcctt ataccacaat attcatgcac
     9781 ttttcagaga tggaaacacc ctttggatcg gaactttttc caaaggtttg aatcggtatg
     9841 atttgaaaac gaaaacactc aaaatctatt ataaaacgag tcagccaaat tctttgtcgg
     9901 aaaacagcgt tttctccatt tgcaaagatc gacagaacac gttatggata ggaactttat
     9961 cgggattgaa tattttcaat taccaaaacg attcatttac ccgaattaaa gaattggaag
    10021 gagcttttgt tcaggatatt tttgaagata cggatggaaa tatctgggtt gctacttttg
    10081 taaaaggttt gtttctgttc aatcccaaaa ctcacacatg gaaaaacttc gttcacactt
    10141 cgaatgccgg cagtcttcct tacaacaaaa cgacttctgt atttgaagac agcaaaagga
    10201 gaatttggat aacaactgaa ggcggtggtt tttgcaggta cgacaaaaaa aatcaatctt
    10261 tcgtaactgt caacagcacc aacggtttac ccaatgatgt ggtttatcaa atacaggaag
    10321 acaattcggg caatttgtgg ctttccacca attccggttt ggtaaaatat gttcctgaaa
    10381 cgggaataat ggaaaatttc acagtcaacg acggacttaa aaccaatcag ttcaattaca
    10441 aatcgagtta caaagctccg gacggaacgc tttattttgg ttcgattgat ggttttgttc
    10501 gtttcaatcc tgcttatttt aaggaaaaca acctcttgcc tcccattgtt ttaaccgatt
    10561 tctatttaaa taattcgccc gccaatgttt cggatgcaga ttcacctttg aaagaaaaca
    10621 tttcgtttgc agaaaaaatt cgacttcctt acaataaaaa ttcttttacc attaaatatg
    10681 ccattttgaa ctattccggt ttacgcccta aaaatgttgt ttacaagctg gaaggattcg
    10741 acaggcaatg gattccggcc aatggttctc aatcggttgt ttattccaat ttgaatcccg
    10801 gaaaatatcg gttgatgatc tctttcgata caaattcctc aggtggcatg aacaaagtca
    10861 gtaaaacatt ggatattgtt ataaaaccac ccttttggga aacatggtgg gcctatttgc
    10921 tgtattttct catcactatt gccggaattt ttgagttgat caattatttt cgtcagcgga
    10981 caaagagaat tcatctggaa aaaatgcgag atttcgagca ggaaaaggaa aaagaactct
    11041 atgaatcgaa aatagatttt ttcaccaatg tagcgcatga aatcaggact ccattgtcgt
    11101 taattaaagc tcctttggat caaattttgc gaaacgatga aaccgttact cccgatgtga
    11161 aggacaattt gaaagtaatg agtaaaaaca ccgatcggtt actggattta accaatcagt
    11221 tactcgattt caggaaaacg gaagccgaac tgtataaatt gaaagagaaa cgcaccaatt
    11281 tgtcggaatt gatgaaagaa acctttgacc gtttcacccc gtttgcaaaa caaaaaaata
    11341 tttcattcca tttaaacctt cctgaaaatg aagtttttac aaaaattgat gctgaagcgt
    11401 tcacaaaaat tattagcaat ctgatcaaca atgccgtgaa atattgcgac agtatggtga
    11461 acatttcgct cgaagtagtg gctgaaaatg aaattgaaaa actcgtattt aaaacagaaa
    11521 acgatggagc tattgttccg gaacgtttta aaaatgaaat atttaagccc tttacacgat
    11581 tgaataagga aaaagataaa atagttaccg gaacgggcat tggtttggct ttgaccaagt
    11641 cgttaacgga acttttaaag ggcgacatta cttacaaagt tgaaaatggt ttgaacgtgt
    11701 ttcgggttac tctaccattt aatcctttaa ccggcaacga gtggattgaa gaaaagaccg
    11761 aaatgcagga aacgcccagt agaaaagagg aagataaagt tgaaaaatcg ttaaaacggc
    11821 cgttaattct tttggttgaa gatgataaag ggatgatgga atttttgaca aaatgtttga
    11881 agagggaata cagtgttctt tctgcttcgg atggtaaaga agctttggag gttttgaaaa
    11941 ataaaaatat cgaccttgtt gtcagtgatg taatgatgcc cgagatggat ggttttgaat
    12001 tgacatccta tatcaaatcg catatcgaat acaatcatat tccggtaatt cttttaacgg
    12061 ccaaagtaaa cgttcaatcc aaagtgcaag gttttgaagt gggagccgat gcctatatcg
    12121 aaaaaccttt ttcggtggac gttttactgg ctcaaatagc aagtttgttg caaaataggg
    12181 agaaatttcg cgaagctttc ttgaaaaatc cttttaaggg actcaatcac gtagagttga
    12241 ataaatccga tgaagaattt ttgcaaaaac tggacgacat tattcaggaa aacatatcga
    12301 atcctgattt taacattgaa gatttgtcgg aacatttctt tatgagccgt gccagctttt
    12361 acaggaaaat aaaaggaatc ctcgatttga ctcccaatga atatcttcgg gtggcaaggc
    12421 ttaaaaaagc agcgcaactc ttgaaggaag gcaaccaacg aataacggaa atttgttact
    12481 tggttggatt taattctcct tcttattttg cgaaatgttt tcagcaacaa tttggcgttt
    12541 tgcccaaaga tttttcgtaa attctgctat tttgagatga tatttttatt tttcgattca
    12601 tttttttctt ttgaatattg aattttaagc taagtttgct tgacaaagaa tactaaaaat
    12661 ttagccaatg ttgagaataa aattattatt gttaagttca ctgtttagtg tcattacttt
    12721 tgcccaatgg cagccagccg gagaaaaaat taaaacacgc tggtcgtccc agattgatgt
    12781 caataatgta ttgcccgaat atcctcggcc tttgatggaa cgttcggaat ggatgaatct
    12841 caatggttta tggaattatg ccattcttcc ggttgggaaa ccagctcctt ccgcttttga
    12901 tggtaaaatt ctggttccat ttgctgttga atcaagtttg tcgggtgttc agaaaaatgt
    12961 aggaaaggca aacgaattat ggtacgaacg tgaattcacc ataccttcaa aatggaaaaa
    13021 taatcgtgta cttctccact tcggagcggt ggactggaaa accgacgttt gggtaaacga
    13081 tgttaaggtt ggtcggcata ctggaggata tactcctttt tcatttgaaa ttactactgc
    13141 actaaaaaac ggttcaaaca aattgatagt taaggtttgg gatccaacca atgatggtta
    13201 tcagccacgt gggaagcaag tgaataaacc cgaaggcatt tggtacacgc cagtaaccgg
    13261 catttggcaa acagtttggc tcgaacccgt tccggataaa tttattactc aggtaaaaac
    13321 aacaccaaat attgatataa atattttgaa tattgaagtt gctacaaacg atttttctgc
    13381 ttcggacaga attgaagtaa aagttatgga tggctcaaaa gttgtggcta cggctcagtc
    13441 catcaatcat cttcccgtag aaattgttat gcctgatcaa gtaaaacttt ggtcgcccga
    13501 gagtccgttt ttatacgatt tggaaatcac gctttggaac ggaacaaaac aaatcgacaa
    13561 agtcaaaagt tatgctgcca tgcgaaaata ttctatcaaa cgggatgaaa atggaattgt
    13621 tcgtttacag ctgaataata aaaatttgtt tcaatttggg ccgctggatc aaggttggtg
    13681 gccggatggc ttatacacag ctccaacaga tgaagcatta aaatttgata ttcagaaaac
    13741 caaagatttt ggttttaaca tgattcgaaa acatgtaaaa gttgaacccg cgcgctggta
    13801 cacttattgc gaccgtctgg gcatgattgt ttggcaagat atgccaagtg gtgatggtgg
    13861 cccgcaatgg caacctcatc agtatttcag cggtttggaa tggcttcgtt cgcccgaatc
    13921 cgaagcgaat ttccgcaagg aatggaaaga aataattgat tttctgtatt ccaatccgtg
    13981 tataggcgtt tgggtgcctt tcaatgaagc atggggacaa tttaaaacca aagaaattgt
    14041 agaatggacc aaagagtatg atccttcacg attggtaaat cctgccagtg gtggcaatca
    14101 ttatccggtt ggcgatatgc tggatcttca caattatcct aatcctgcat tgtatctgta
    14161 tgatgcacag cgtgctacag ttttgggaga atatggcgga attggctggg ctgtgaaggg
    14221 acatttatgg gaacctgatc gcaactgggg atacgttcag tttaacagtg ccaaagaagt
    14281 taccgatgaa tatgtgaaat atgccgaaca actgatgaat ttgattctgc atggtttttc
    14341 cgcagccgtt tatacccaga ctactgatgt tgaagtggag gtgaatggtt tgatgactta
    14401 cgatcgtgaa ataataaaaa tggatgaatc gagagtcaaa cagataaatc aaaaaatatg
    14461 caatatttta aagaattagc catttgtata tctgttttac tttgtttttc gtgtcagaaa
    14521 aacaacgaaa ttgcagcgaa atcaccttca ctgtctattc gatgggatga aagttcgttg
    14581 gtgtgtatcg ctgccgaagg cggatatccg cgtgtaaaaa gattgaacga cggttcgctt
    14641 ttggctgcct acgaaaacag aaaaggggat gtgttggtaa aaaaaagtcg ggatgaagga
    14701 aaaacatgga gcgattcggt gcttgttttt ggaagtttcg attatattca tccttcaaat
    14761 ggactttcgg caaaaataaa tattgccaat ccggaattta tccagcttgg gaataacgat
    14821 attttgtttg cctgcaactt tcgtcccaat aaggatgaag tttacccttt ttccattgcc
    14881 gtaaaaagaa gtcgtgataa cggaaaatcg tggagcgaac agcaaattgt ttatcaagca
    14941 gctccgcgat tcattgacgg atgctgggaa ccttcttttc ttattttgcc ggatggcaga
    15001 attcaactct attttgccaa tgaatttccc tatcagcaaa gcaacgaaca ggaaatttcc
    15061 atgctcgaat ccaatgataa tgggacaagc tggtcggccg ataaaaagac agtgagcttc
    15121 cgcaagggat ttcgtgatgg catgccggtt gcaacgaccg atggaaatga aattttcgta
    15181 gccatcgaag acaatggctt tgaccagttc aaacctttta ttgtgaaaaa tgccattgct
    15241 gataattgga aagaacctgt tttagccgaa tctcccaatc gctattcagc tttgaaagaa
    15301 cctcttcctt cttcagttta tgcgggtgca ccttatttga ttcacacaaa agaaggatat
    15361 tacgttttgt cctatcaaac gacccatcat cgaacttcca actgggaatt gtccacaatg
    15421 gaagttgttg tcagcgataa accgtccgat tttgtttcgc cgtcacaacc tttcgctgta
    15481 cctttaagca aggaagccaa atggaattcg ctgtgtgatt tgggaaatgg tgaaattgcg
    15541 gctgtttctt ctaccaattt caaatcggat aaaatcggta tttggatgat taaaggaaaa
    15601 attgtagata atcgaaaata aaaatttatg aaacgttcgc ttggaataat attattgaca
    15661 ctgtttttca tttcgtgtaa gggaagtgag gcaatcattc ccgaaacaga gaatgttagc
    15721 acttacaaaa atcccatcat tccacgaagt ttgcctgacc caacggtaat gaaagcttcg
    15781 gatggcaatt tttatcttta tgcaacagaa gatatccgta atgttccgat ttttcgctcg
    15841 tccgatttgg tgaactggaa ttttttggga accgctttta ccgaccaaac gcgaccgaca
    15901 tttgtttcgg gtggtggtat ttgggctccg gatatcaatt atgtcaatgg aaaatatgtg
    15961 ctttattact ccatgtctgt ttggggtggc gaatggagtt gtggcattgg agttgcaaca
    16021 gccgatcatc ctgaaggccc gtttaccgat cacggaaagc ttttcattag tgatgaaatt
    16081 ggggtgcaaa attccattga tccttgttat ttcgaagaaa acggcaagaa atatctgttt
    16141 tggggaagtt ttcgaggaat ttatggtatt gaactttccg atgacggatt gtcggtaaaa
    16201 ccgggagcac agaaagtgca gatagcagga actgcttatg aaggaacata cattcacaaa
    16261 cgaaatggat tttattattt gtttgcttca atcggaagtt gctgcgaagg attaaacagc
    16321 acttacacag cagtagttgg acgttctgat aacctttttg gaccttattt agataaatca
    16381 ggacaatcca tgctggaaaa tcaccacgaa attttgattc agaaaaacag caaatttgtc
    16441 ggaaccgggc ataattcgga aattgtttcg gacgataaag gacaagattg gatattttat
    16501 catgccgttt gggtggacta tccacaagga agagtgttaa tgctggataa agttcaatgg
    16561 atgaatgatt ggccttatgt aaccgataaa tgtccgtcat tagaagctga aaaaccgtat
    16621 ttcaatgaat aattgttcaa taaaaaagtt ctatatattg atccgataaa acaaaaaagt
    16681 catgagcaag aaattgaata tcgttttttt aatgttggta ttgctgcttc cgttgcaggg
    16741 ggcaaaaaca gtttcgacca aacagtcagt ttggacaaaa gaaaaagcca atgcatggta
    16801 tgcttcgcag ccgtggttga gcggttgcaa ttatatccct gcgtcagcca tcaatcaggt
    16861 agaaatgtgg gcaaaggaaa catacgatgc accaacgatt gacaaagaat tgggttgggc
    16921 tgaagaactt ggattcaaca ccatgcgtgt ttttttgagt agtgaagtct ggaaagacga
    16981 tccttcggga ttgaaaaaac gaatggacaa cttcctttcc atttgcgaga aacaccacat
    17041 tcgtcccatg tttgtatttt tcgacgattg ctggaatgaa gaaacagtta aaggaaaaca
    17101 accggcaccc aaacccggca ttcataattc agggtgggta caggatcctg cctgcagcct
    17161 gagagccgac actgctacac tctttcctgt actcgaaaaa tacataaaag atatcgtttc
    17221 cactttcaaa aacgatcaac gtattctcat gtgggatttg tacaacgaac ccggcaacag
    17281 ccgacatgga attaattcgc tgccactgct gaaaaacaca ttcaaatggg cacgtcaatc
    17341 gaatccatcc caaccgcttt cagccgggat ttggtatttt ggttgccccg aactcaatga
    17401 atttcagttg gaaaattcgg atgtggtaac ctatcacaat tattcggatg aaaaaaatca
    17461 cgaattatgg atcaatttcc ttcggatgca aggaaggccc atgatttgta ccgaatacat
    17521 ggcacgacgg aacgacagtc atttcgaaaa tatcatgccg ttgctcaaac gcaataacgt
    17581 aggagccatc aattggggtt ttgtttcggg taaaaccaat acaatttttg cttggagcga
    17641 tccgcgtccg aacgaaaaag aaccgaccgt gtggttccac gatatttatc gtcaggacaa
    17701 aacacctttc gacccaaagg aaattgaatt tatcaaaaag atgaccggca aagagtccaa
    17761 tgtgcagctg atgaaacccg aaaatttcaa tgaaaaaatt gatggcaaac agatatcgct
    17821 tttcaccttg aaaaatcgtc agggaatgac agcccaaata acgaattacg gaggacgagt
    17881 tgtgtctctt tgggtgccgg acaaaaaata cgatttcaag gatgtgataa ccggatattc
    17941 ctcgctgaaa ggattccaac aatccactga agtttatttc ggagcattaa ttggaagata
    18001 tggcaacaga attgccaaag gccgatttga attgaatgga aagacttatc agctcccgat
    18061 caacgatggg gaaaaccatt tgcacggtgg acccaaagga tttcatacgg taatatggga
    18121 tgcccgacca ttcaaaaatg ccaacaatga agatgctttg gaactgaaat acttttctcg
    18181 tgatggcgaa gaaggttatc cgggtaattt gacggttaag gtggtttata cattgacaaa
    18241 cgacaacgag ctgaagattg aatattctgc cacgaccgac aagccaacca ttatcaatct
    18301 gaccaatcac atgtttttta acttgcatgg attttcggat ggaattgcga aatcgatcaa
    18361 ttcacatgtg ctggaaataa atgcgtctaa ttatacaccg acggacaaag gattgattcc
    18421 aaccggaatc attgctccgg taaagggaac gccaatggat tttacagtac caacgccaat
    18481 aggagaaaga gtaaatgaaa actttatcga tttaatcaac ggaaaaggat acgaccacaa
    18541 ttgggtattg aataaaaaag gagataaact gacagaagct gccgttgttc acgaaccttc
    18601 caacggcatt caaatgcgag tgattaccga ccaaccggga gttcagtttt acggaggcaa
    18661 tttcttgaat ggaacagaaa gaggtaaata tggcgaagtc tatacgtatc gcacttcatt
    18721 tgctatggaa acccaacatt tccccgacag tccgaatcac ccgaactttc cttcaacggt
    18781 tcttaatccg ggtcaacagt atcaacatgt ttgcatttat aaatttgaag taggcggtaa
    18841 ttaaaagcac tgcctccaaa agatgtgttt tcttaattgg tttaaataac agttgtttcc
    18901 tttaaaagat ggttaataat ccgatatttg tttaccaatg aaaacttatg tttcaggaac
    18961 ttattttctt gaattgcatt caaatggaaa gatttaccat cagactatca ttaaaaagta
    19021 atgttccaaa ttctcaaaat tcttaaagag ccggttgttc aggacggctc tttaattttt
    19081 cagacaaaaa acttgaaatt cttaaaattt taatctggca gaggcaagaa aattggttcc
    19141 ggcctgtgga aaatatctca gttcgttatg ccgttcgttg cccagatagc aggaatacca
    19201 tgtgtaaccg ttcgtttcgt atttttcgtt gaagagatta ttgatttgaa cgagaaattc
    19261 gagcgttttg ataggactga tagcaagcga gtatttcaaa ttcaaatttt gaacaaaata
    19321 tgggtcaata gaacgttgaa tatccgaagt gttgtctata tactgtcgac taacgtaatt
    19381 tgaatgaaaa gccgcttcga aaggcttaag atgaaaagaa aaaatactgt tagcaatcac
    19441 gtttggagaa taggctatat cggttgtccc aagttcgttg ttgcgtgtgc cgatatagtt
    19501 ccagttttca tcatacacgt cgatatcctg ttcagtaaaa tttttgattt tattgtgact
    19561 aaaagtgcaa tttccgtccc agtttaaaaa gttagtaatt ttccaacctg ctgtcagttc
    19621 aattcctgcc cgatagctgt cgggaatgtt agtagtcaga aattctccga tttcacttac
    19681 ttttcccgtg agaatcaact gatttttata tttcatatag taaaaattgg tgccggccga
    19741 aaatcgggac gatcggaaac gatagccggc ttcggtatcg tataatgttt cgttggttgg
    19801 acgttcgttt gctccggctt cagtaaaatt gttcctgttt ggttctctgt gagatacgga
    19861 ataggaagcg taaaattcat ttcgggtact tggttgccat gtgaatccga atttcggatt
    19921 gaaaaaatgg taaatgtgct gttgggtaat gtcccgcatg gattgattta ttttatcata
    19981 tttgtcatcg gttccgtcca tccgatagtt aatatatcgg tattgcaaat caattgaagc
    20041 aaacaagttt ttaaaagttt cggtgttcaa cttggcataa atattggcat cgtcttttat
    20101 ggaactactt ctgtaccagt ctttcccaat gtccaaatta gctgcattgc gaacccaaat
    20161 tatttttccg taatgctctc catcataccg gttgacagct ccgccaagcg aaaagttcgt
    20221 gttttttttc tcgtaattca aagctccggt aatgccatag aaatagttgt cgagccattt
    20281 ttgccgaacc aaatcggtcg ttttcagcgt atcaaaacct ataatggcag gtgtgagccc
    20341 atattctgca tattttttgt cgattttata ctcttcataa taaccgattc cgcgagtcag
    20401 atgggtagct acatttgaat ggagatggct gttccatttt tgcaaccagt agagatgata
    20461 atgagtttgc aaataattgt cggtttggtt gtcgtagaat tttaattctc cattgttgtc
    20521 ggtataggcg cccaaatcgt tgtatctcct gtttgttttc attatttcgg catctacacc
    20581 gttccatgcc tgataagttt tttcttttcc tccgaaggtg agcagtcgca aaactgtttt
    20641 atccgaaaaa tagccgccgg aaaagtaata ggattgcata tctaccgaag cgcggtcgat
    20701 ataaccatcg gaagttacgt tcgagaggcg agcatcaaat gcaaaattgt tgtccagcat
    20761 gcccgatcca actttaatgg agttcttgtt tgttgcaaaa gagcccaaag aggtgctgag
    20821 gtcggcatac ggttccaatg aaacactttc ggtctgcata ttgatgctgg cgccaaaagc
    20881 agctgcgcca ttggttgaag tgccgacgcc ccgttgaacc tgtaccgatg atagtgatgc
    20941 ggccagatcg ggaaaatcga cgaaaaatgt tccgtgcgat tcggcatcat tcaacggaat
    21001 accattaata gtaacatttg ttcggtttgc atctgttcct ctgattctaa atcctgtgta
    21061 accaacaccg tttccggcat cggaagttac tataaatgaa ggtgtcagtg ccaaaagata
    21121 aggaatatcc tgaccgagat ttcggctttc tatttcctct ttttttattt cactgtaagc
    21181 aatgggcgaa cgctcattgg cacgcaacga agttaccgta atttcatcta tttcaatgga
    21241 aattcgattc atttgaatcg tgatttcttt atccggtgtc gtttcaaccc attgggtttc
    21301 gtaacctaaa taggaaactt gcagaaaata atttttattt tttaaatttt caaaaagaaa
    21361 agtaccattt ttatcggttg ttttaccgat tccttccttt tgcaggtaaa tatctgcccc
    21421 aacgagggga tgaccatttt catccaacac ttttcctttt atccagtgtt gaccaaagga
    21481 cattaacgtc ccaaacagca aaattgccgt aaaaacaaat tgtctcatca caaaaaatta
    21541 attaatggtt aatactttgt tatgagagcc taaaaaacgg ttgattgaaa acttattccc
    21601 tactcggaat tacccgtgca ggttcaaagg gtatgatctc agcccgaaat tattaaggca
    21661 ccccatttta ataaaaaaat taagttgatg aagaaaaacc gcggatttct ctttttcaag
    21721 ttgcaaagtt acaaaatctt tcccaataaa agtttaaatt caagaaattg atattttttt
    21781 atcaaaaaat tatacgaagg gaaaaagtcg tttttaattt tttctattca attgaatatc
    21841 aataatataa ttgaaaaagt tgtattgatt tgctgaaatc aacatgcatg aaatacagta
    21901 aaaaaaacga tagaaaaact acaaagggaa ccaaaaaata agtaactaat tagaaaacaa
    21961 ctttttatat tcgaaattcg ataaaagtgc aaatgacagg aaaaataaaa attctgtccg
    22021 gtgaatttcc agcattttgc cggacagaat tttttttgat gcaaagaatt tattcttcac
    22081 gcaatttgac tcgaaagtcg aaattaatcc tccaattttt ttatcagcaa ttttacggct
    22141 gactccacat ccgaactttc ctgcaaagct ttgatgaaag cactgccgat aattacacct
    22201 gaagagtaac gcgaagcggt ttcgcgggtc aatttgttgg aaatgccaaa gccaatgagt
    22261 cggggatttt tcaaatgcat cgaattaatg cgattgaaat aatcaatatg atggtcaaat
    22321 gaattttggg tgccggtaac gccggcagaa gaaaccatgt aaataaaacc atgcgtgtgt
    22381 tcatcaatct gacgaatccg ttcttctgag gtttcggggg taatcagaaa aataaaatcc
    22441 aagccgtttt tttcgactat ttctctgtat tccgtcagat aaacgtccat aggcaaatcg
    22501 ggaataatca tgccatccac tccgactttt ttacattcgc tgcagaaatt ctcaaaacca
    22561 aactgcataa tcggattcag ataacccatc attaccagtg gaatgtgcac cgaagcacga
    22621 atgttggcaa gctgactgaa aagtttcttt agattcatgc cgtttttcaa agctatctgg
    22681 ctgctgtttt gaataacagg tccatcggca agcggatcgg aaaaaggaat accgatttcg
    22741 gccatatcca cactgtattt ttgtaaactc tccagaattg gaactgtgtc attcggtttt
    22801 gggaagccgg ccgtaaaata aaccgagaga atgttttctt ttttttgcgt gaataactgt
    22861 tgaattctat tcataatgaa ttatttatgt atttttcttg ttaaagtgaa cgataccgat
    22921 caataaaatt gcttaaaagt tcgacatttt ttaagccggg cgatatttcg aaacggctgt
    22981 tcagatcgac gcctgcaaaa gccggatgct gaattttatt aatatcttct aaatcttcaa
    23041 tcgaaattcc gccactgaga agaaaaggtg ttttttcttt gtaattttcc agcaactgcc
    23101 agtcgaattt ctgcccggcc ccgccataaa tgggagtttg tgcatcgaaa aggaaatagt
    23161 cgcaactttt ttcgtagcgt ttcattacct tgaaattata agcttcggca acaggaaaag
    23221 ccttgatgac cagcaatccc gtatcgtgca attggttgca cattttcacc atttcacttc
    23281 catgcagttg tacgccgtct aaatggtatt tgtaaaccgt agccaggatt tgatcgagtt
    23341 tttcgttgac aaaaacacca attttaccga tttcgggagg caattcattc aaaacggaaa
    23401 catttaaagg ttcggcataa cgcggcgatt tgggataaaa gataaaaccc atgaaatcgg
    23461 gacggagacg ggctacggca cgaatattct ccgggaattt catcccgcag acttttatca
    23521 attttttctt tgtcgtatct gcaaaccgag acggaacaat gttgtttttt tctactgatc
    23581 tcataaagaa aattcaagta atgtaacttt tgcagtgatt aagaggttta acaaaattat
    23641 ttgtcaggaa aatatttatg aaacaaattt acaaatatat cgactaatcg agtcaataat
    23701 ctaaagctgc taattatctt cattcaattg taatattatt catttaactg tctcaaaaac
    23761 tctttcagag caagagccgg atggacttcc ttcataaaat tttcacccat cagaaagccg
    23821 ctgtatccca atcgattcaa ttctttcaca gtttcgacat tggaaatgcc actttcactg
    23881 atttttacaa attcaggagg aataaacgct gccaaatttg ctgaaatgcc cgtgtccacc
    23941 tgaaaagttt taagatttcg attgtttacg ccaaccatat ctacattttc gttcaaatag
    24001 ctcaattctt ccttattgtg cacttccagc agaacttcaa gtcccaattg gtgagccaac
    24061 cgtgaaaaat ttttaacttc ataaatggtt aaggctgatg caatcagaag aatcacatcg
    24121 gcgccaatgg ctttggcttc atagatttga aattcatcca ccacaaagtc tttccgtaaa
    24181 atgggacagg aaattttatt gcgaacggtc gttacatctt caatatttcc accaaaaaat
    24241 tcggtatcgg taaggataga aattcctgaa gctccttcca cattgtagcc gatagtaact
    24301 tcttgcacat tggcatcggc atgaatccaa cctttggaag gagatttacg tttgaattcg
    24361 gcaataattc ccaaagaaga attttgtaat ttctgtttca gcgaaatagt gcttcgatca
    24421 aaaaaattag aattttccaa ctcggtaatt gacacattta gctttcgttt gttaacttcc
    24481 tttattttat tcagaagaat attattcagg aaattttctc ctgggttcgg taagtgattg
    24541 ttttttaatg ttgaatcagt cattggtaga aatcataaat gatatatttc ttcaaatttc
    24601 acaaatgctt tttttgcttt tccgcaggcg agcgatgcga tggcttctgc cttgcattct
    24661 tcgagcgatt tttcggggca gcgcgtttgt atggcaaatg cagaattaat gaccactaca
    24721 tttcgctggg catcggaagc tttgttatcc aatacattca taaatatttc ggctgcatgt
    24781 tccactgttg tgccacccca caattcttcc tgctttactc gctgaaagcc gagttgttgg
    24841 ggactgtaaa catattcgcc aaagttgttg attattttgc agtcgtcggt aagtgaaact
    24901 tcatcatatc cgtcgagagt gtaaacaatg gtataatttg ttcccaaccg ctgataaata
    24961 tagttgtaca agcgcatcat tttcaggttg taaactccca agcactgata acgcggtcga
    25021 atgggactga tcaacgggcc aagcacattg aagaaggtgc gaacgccgat atttttacgg
    25081 acgggagcaa catttttcat tgccggattg cagaatggag catgcaaata ggcaaaaccc
    25141 gattcttcca gtgagcgccg tacaacatca gtatcggtgg taaatttagc gccgtaatac
    25201 tccaacacgt ttgaagagcc actgatagat gttgctccat aatttccatg tttggcaact
    25261 ttgtaaccgg caccggccaa aacaaaacaa ctgcaggttg aaatgttgaa ggtgtttttt
    25321 tcgtcaccgc ccgttcccac aatatcaatg ggatcgaaat ccgataagtc cattggaata
    25381 gccattgaca tcaaggcatc acggaacccg atcagttcgt ccagttcgat gctgcgcatt
    25441 agatataccg aaataaaagc ggcaatttgc gattcgttgt acatgccggc tccaatattt
    25501 ctcattactt cggccgattc ttctctggtc aacgactgat gattaaataa tctgtttaat
    25561 atctgtttca taagttaact gtttttttgc tgaaaatgag gaaaaaactt gttttctctg
    25621 atttgtttct ctataatttt ttaaatctat tatttccccg ataaagggaa ttccaaccaa
    25681 tttttcagta tttgttcacc ggaaggtgtc agcaccgatt cgggatgaaa ctgaacgccg
    25741 cgaacatcaa gggtgcgatg tttcagcgcc atgatggtac cctgctcgtc ctttgccgta
    25801 acctgcagac atggtggcag tgtagaatcg tttacaaccc aggaatggta tctgccggca
    25861 ataatttttt ccggcaaatt tctgaacaaa acttcatcgt caaaaatgct gacttcggaa
    25921 gcaatgccgt gataaacctg aggaagattg agcaagcttc caccgaaagc ttcggcaatt
    25981 gcctgaacac ccagacacac gccaaaaatg cttttggatg aagcataggt ttttatcaat
    26041 tcgagtaaaa tgcctgattc ggaaggaacg cccggaccgg gagaaagaat aattttgtcg
    26101 aaacggttta tttcttctaa cgaaatccga tcattgcgat gaacttccac atcagtgaac
    26161 cccaactttt taactatgtg taccaaatta taggtaaagg aatcgtaatt gtcaaaaacg
    26221 agcaatttca tacaggttgc attatttttt cggtctattt aaagttttcg gagaaaattc
    26281 tgaaacttgt tcgataggcc atcggtttat taaaatctat tctgtttgat agttatttct
    26341 ttttccggcc aatataatgg cttgtttcag tgcagccaac ttattgttta cctcctgcaa
    26401 ttcgttttct tcatccgatt tggccacaat gccggcacct gcctgataaa aaagcgtgtt
    26461 gtttttgctg atgaaagagc gaatggtaat ggcttggttg aatccgccat cgaacccaat
    26521 gtagccaatg caacctccat aagggccgcg gtcgtgtggt tcaatttgat cgataagttg
    26581 catggcttta atttttggag cgccggaaag tgttccggca ggaaaagtat cggcaaaaaa
    26641 tttcatgatt tttgttccgg gacgcagttt cccgctaacg cacgaaacca gatggataac
    26701 atgtgaataa tattgcactt cccgaaaagt ttcaaccttc acttcgtcgg catttcggct
    26761 cagatcgttg cgtgccaagt ccaccagcat tacatgttcg gcattttcct tttcgtcgtt
    26821 tttcagtttt tcggccaact caaggtcttt aatgtcgtct ccggtgcggc gaaaagttcc
    26881 ggcaatgggc tttatgtagg ctttttgagc tttggcatcg actaccaaat gggcttcggg
    26941 cgaagaacca aaaatgcgga aatcggcaaa atcgaaataa aaaagatagg gagaaggatt
    27001 gatagaacgc aaggtacgat aaagcatgaa atcgtctccc tgatattgct ggtagaaacg
    27061 tcttgaaagg actatttgaa aaacatttcc cttgaaacac tcttcctttc cgcgttttac
    27121 catccgacgg tagtcctcgt cgttaatatc gcatttttcg ctgccaattc ccgaaaattc
    27181 gtaggcggca atattgttgt tgaggagtat ttcttctatt tcgttcatgg ccgacttttc
    27241 gttctcgagc aaattttcaa tgattgtcaa ctcattttta aagtggttga cggcaatgat
    27301 atatttgaac agcacataat gcaaacccgg catacttccg ggaacagttt cgcgtgccga
    27361 aagccggatg tcttcaaaat actgcaccgc ttcataagaa gtatagccaa aaagcccgtt
    27421 tactacagcg ggaacttcgt tgtcctgttc gatacgttcg aatgatttca gaaaaatatc
    27481 aaaacggtcg gctactgtca tcttgtccgt tacgggagtt tgaatttttg taccatcggg
    27541 gtaatattca tgtaccagga aatgattgac ggaaattcct ccaatgggag aaaacgcaat
    27601 gaaagaataa ctattttcgg ccgcatgata atcggaactt tccaacaaaa ccgaatgagt
    27661 gtataaatct ctgattttta gataaatgcc aaccggcgtt tcnnnnnnnn nnnnnnnnnn
    27721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27781 nnnnnnnnnn nnnnnnnnnn nntgagccga ttcgatagcc ggaataattc cttccagacg
    27841 agtcagttca aaggctgctt ccatagcttc atcatcgttg atggcataaa ctttggtacg
    27901 accaattttt gtaaaaaaag catgtgcagg tccaacgccg ggataatcca aaccggcaga
    27961 aacggaatat ggttcgtcaa tctgtccgtc ttcattctgc aaaatcaagg tttttgctcc
    28021 gtgaataatg ccggtttttc ccaagtgaat ggtagcagcc gtttttccgg tctcaattcc
    28081 cattccgccg gcttcggccg aaatgatatt gaccctttca tcatccagaa aatgaaaata
    28141 agttcccatt gcgttgcttc ctccgcctat gcaggccatc aaataatcgg gataatttcg
    28201 tccaattttt tcctgcagtt gccatttaat ttcttcgctg ataaccgatt gaaaatagcc
    28261 aaccatttca ggatacggat ccggaccgac tgttgatccg ataatgtaat aagaatcggg
    28321 atgcgaacac cagtaacgaa tggcttcgtt ggttgcatct tttaaggtca tgtttccgct
    28381 ggtaacaggt tctactcttg cacccagcat tcgcattttc tgcacgttca gatattgacg
    28441 ttcgatatcg gtttttccca taaaaacggt acaatccatt cccatcaaag cgcaagcagt
    28501 tgcagcagcc acaccgtgtt gtccggctcc ggtttcggcc agcatgtgcg ttttacccat
    28561 ttttcgggcc aatagtactt gtcccagtgc attgttgatt ttatgtgcac cggtatggtt
    28621 caagtcttca cgtttcaggt aaatattgac accatataat tcggaaagtc gccgtgcaaa
    28681 atacagaggt gttggtctgc caacatagtt tttgagtaaa tcgtaatact catttttaaa
    28741 gttttcatcc ccttttatgg ataaaaaggc ttttttcaac tgctcgacgg aagcatatag
    28801 aatttcagga atatacgctc cgccaaattc accataaaaa cctttttcat ctggctggta
    28861 aagttttcta tcagctccgt ttctcgattt ttgactattt tcgggcatag tttaattgtt
    28921 ttttgattgt atttttatca atgaaaaaag gcttgtcgtg agttcccgac aagccttttc
    28981 ataaatattt ctttatttca actatcaaca taggtgtccc ttctcacgat ctgaatattc
    29041 gcaagcgcca ccaccaaaat ttgttgattg tcgaatttgt tttcattttt tacagtctgt
    29101 tttgttttcg attgcaaagt tgacaaatta tttttaattg tgcaacaaaa aaatcaattt
    29161 ttaattcaga aaattattaa tatccaactt acaagtattt tgctcaataa atcatcctca
    29221 tcttacttgc aaaaagtgta atttaatatt ttgaat
//
LOCUS       DAOTAX010000023        28022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1393_length_28022_cov_31.124361, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000023 DAOTAX010000000
VERSION     DAOTAX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28022
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_28022_cov_31.124361"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLM04_04035"
     CDS             complement(<1..397)
                     /locus_tag="PLM04_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02281.1"
                     /translation="MKQFRITILLIATTLLLCACPEIEEVHRNIVFVNKSGKRVAYQF
                     AFSKIQDIYEDTVFNCNKTSEGFIENDSSFILDCPIRERSWEISLGNSYYIQYLVLDG
                     DKFSQYYTEPCDTIRKYVPVLHTYRLTLAD"
     gene            complement(978..1619)
                     /locus_tag="PLM04_04040"
     CDS             complement(978..1619)
                     /locus_tag="PLM04_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02282.1"
                     /translation="MKTRIVIIFSLVYFSFLQIYAQECISIFVEDIQKQSFNLKINEK
                     KAVKQFMKVGYKVHPNYKLQDSNVIAKPIYSIFKKKHYPQKMNDIRNILCYLDTKRLY
                     IDEIYFYKDSVFYGSADGAYSNYPDQVIGKSDFGVKLASIILKENPDLIFTIGQTNGY
                     YLFFKNNQLNVYSMFYNKEKKFIKYEIDDFINKYMDEFDLIIGTDYSPPIYCR"
     gene            complement(1623..1940)
                     /locus_tag="PLM04_04045"
     CDS             complement(1623..1940)
                     /locus_tag="PLM04_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02283.1"
                     /translation="MIKPNISPSKNNIRYYFGTYEKDIDKFGNITQTDYIYTPAGVIG
                     TDQMAGHAVNVVSFKVADKLNWFGGGFTPFLKMNTVSVWDPLSASVRRLTEEILKLEF
                     IKY"
     gene            complement(2208..2489)
                     /locus_tag="PLM04_04050"
     CDS             complement(2208..2489)
                     /locus_tag="PLM04_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02284.1"
                     /translation="MNIKQLKRELKKQLKKEKYPKTFIYYKWDLLKFKHAKYFELLLP
                     TESYCLEEKNNKWEVYYIERGTKLYIKEFSSENEACEYFYNWIMQRNSR"
     gene            complement(2871..3170)
                     /locus_tag="PLM04_04055"
     CDS             complement(2871..3170)
                     /locus_tag="PLM04_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02285.1"
                     /translation="MQLLRILADLTFYSSNIIGKDRTYFNGYRPAFKIREDMFNSGQI
                     EFIGQDKTISGSKNIKAYIIFSHGDLLLDFITIGQRFTFGEGSETLGEGIVLNIL"
     gene            complement(3174..4436)
                     /locus_tag="PLM04_04060"
     CDS             complement(3174..4436)
                     /locus_tag="PLM04_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HON02286.1"
                     /translation="MVKNGTLYYVHTDNLGSIQAITDENKNIVSSYYYTPWGGRVLLS
                     GANITDRGYTFHEHLEPFGLINMNGRVCDPVLARFLSPDNYVQAPDFTQSFNRYSYCL
                     NNPFKYTDPDGEFWHIIVGALIGGVVNLIANWNNCEGFWQYVAAFGAGAATAATGGAA
                     GATWVGIAGVSAAGSAAIMGTNSVIAQTGHNFSGMGNVDWGQVGISSAIGCVSGFAGG
                     AAGYWAANSSMLVNGINSPILRSAVVSPLAAGAGHIAGRTTANLFAGQNLSDAFANSF
                     NGLGSSMIVGGAMGVASTVAVNYANNVNPWTGKNLTQATPKTISIYRAVSTDEYKNIN
                     SNGLRPNPDGSGYQEGKLFYKSYQDAVNNTSAYDAAFGQKSIIIKIDVPYNISQNFNY
                     FHMDGYDVIYISTVDLPSVNNVMTINKP"
     gene            complement(4715..5113)
                     /locus_tag="PLM04_04065"
     CDS             complement(4715..5113)
                     /locus_tag="PLM04_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02287.1"
                     /translation="MKKLIYFSILFLFHGCIFTYDPARGLLYVHNNTNEAVYVYLKYG
                     NADSLPLISGLELFTFIDANMKDAYTIGGNRKKPRLPSNENEVTIFFITEKTMRSYDL
                     KEIHKNQMFVKKKTLTNEELENKNWIVTYP"
     gene            complement(5806..8616)
                     /gene="uvrA"
                     /locus_tag="PLM04_04070"
     CDS             complement(5806..8616)
                     /gene="uvrA"
                     /locus_tag="PLM04_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HON02288.1"
                     /translation="MSKQGNIIVKGARVHNLKNIDVEIPRNKLVVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGRMSKPEVDYIKGIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYMKLLYARIGKTYSPISGELVKIHEAEDVVEAAMSYPDDTKLTLLIPIRLN
                     EENNLTEKLRNFQMQGFSRVEAGNEMLRIQDLLLRPELLEKNTEIYLVIDRLVVDHSP
                     STISRLTDSVETAFYEGNGNCILKVNTEEGEKLLHFSKNFEADGITFEIPTEHTFSFN
                     SPIGACPVCEGFGKIIGIDEDLVVPNKSLSVYEDAIVCWRGEVMSEWKKRLIQAAPKF
                     GFPIHKPYYELTESQRQLLWTGNEYFEGLNSFFKFLEENQYKIQYRVMLARYRGKTTC
                     PACKGTRLKKEATYVRVGGKPITELVLMPVEELYDFFEQLSLDGHDAAIASRLLTEIQ
                     NRIRFLLDVGLGYLTLNRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPR
                     DTEKLINVLHQLRNLGNTVIVVEHDEEIIRSADYLIDIGPGAGRLGGNIVFNGQLNDI
                     DKQENTKETIANSYTLKYLYHTEEIPVPTLRRKWNNYIEIVGATENNLKNISVKFPLN
                     VMTVVTGVSGSGKSSLVQDVFYAALKKYYGGVVDHTGQFGVLQGSLQLISQVEFVDQN
                     PIGRSSRSNPVSYIKVYDDIRKLFSEQPLAKQMGYNPSFFSFNTEGGRCEECQGEGTI
                     TIEMQFMADLVLECEHCHGKRFKQDILEVTYRGMNIYDVLEMTVNQAIEFFGQSNNTL
                     EKKIVKRLKSLQDVGIGYVKLGQSSNTLSGGESQRVKLASFLANEKSDPTLFIFDEPT
                     TGLHFHDIKTLLKAFDALIAKGHTVLIIEHNPEVIKCADHIIDLGPEGGDKGGYVVFE
                     GTPEAIVNCGNSYTGKFLKGKI"
     gene            8968..10110
                     /gene="dnaN"
                     /locus_tag="PLM04_04075"
     CDS             8968..10110
                     /gene="dnaN"
                     /locus_tag="PLM04_04075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HON02289.1"
                     /translation="MKFIVSSTELLSHLQTISRVINTKNTLPILDDFKFSLEGNTLTM
                     TASDVETTLITSIQVEESEGEGQVAIASRLLLDTLREFSEQPLTFSINESNLSLTITT
                     SNGAYNFIGENADEFPRLPPFEEEPCSFELPVDNLLSGISKTLFAAAADDLREVMNGI
                     YFDLSPKELTIVATDAHKLSRYKTTNVSTSLDEDEVKSFVLPKKPANLLKNILPREKG
                     NVKIRFDKKYIHFELENYTMVCLQVAGKYPNYNSVIPFNNPIKIIVDRLMLLNSVRRV
                     SVFCNQATNLIKLVFNEQQVNISAEDIDFSISAEETIPCQLEGNAITIGFKATYLIDI
                     LSNIDSSDVVIELSDPSRAALISPLENEENEEVLMLLMPMLLMDYD"
     gene            10218..10988
                     /locus_tag="PLM04_04080"
     CDS             10218..10988
                     /locus_tag="PLM04_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HON02290.1"
                     /translation="MELNLKKPIVFFDLETTGINIVTDRIVEISYHKVFPNGKELTKT
                     KRINPGIPIPAYATAVHGITDADVANCPTFKEVAKEIAADFEGSDLAGYNSNRFDIPL
                     LAEEFLRAEVDIDLMKHRFIDVQVIFHKMEQRTLSAAYKFYCNKDLNDAHSAEADAMA
                     TYEVLKAQLEHYPELKNDVDFLSDFTTQNNNVDFAGRVIYNEKGEEIINFGKYKGKKV
                     EDVLKTDMGYYGWIMNSDFSLHTKKVFTNIKLRQFNNK"
     gene            11224..12048
                     /locus_tag="PLM04_04085"
     CDS             11224..12048
                     /locus_tag="PLM04_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HON02291.1"
                     /translation="MNKIVNKEYLSPNVVKFEVEAPLIAKARKAGHFVIVRVGEKGER
                     IPLTIADANTEKGTITLIVQKIGLSTSKLCELNEGDCITDLVGPLGKATHIDNFGTVV
                     CACGGVGTAPMLPIAEAMKKAGNHVITVLAARTKELIILEEQMRKFSDEVIIMTDDGS
                     YGNKGLVTDGVEMVIKRQKVDLCVTIGPAIMMKFVSELTKRYNIPTIASLNTIMVDGT
                     GMCGACRISVGGKTKFVCVDGPEFDAHQVDFDEMLIRLNGYKDIEREQLHKISPNN"
     gene            12116..13582
                     /gene="gltA"
                     /locus_tag="PLM04_04090"
     CDS             12116..13582
                     /gene="gltA"
                     /locus_tag="PLM04_04090"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HON02292.1"
                     /translation="MTNFNEERMQPWRVELRNSMKPKDRMAIARVKMPELDPEYRSHT
                     MKEEVNLGLTPEMALMESKRCIDCANPTCVQGCPVAINIPKFIKNIERGEFLEAAKTL
                     KETSVLPAVCGRVCPQEKQCEAQCIHLKTKNEAVAIGYLERFAADYERETGNISIPEI
                     AAPLHKKVAAVGSGPAGLAFAGDMAKMGYEVVLFEALHEIGGVLRYGIPEFRLPNKIV
                     DAEIRNLIAMGVHFVTNCIIGKTITIDDLLAEDYKGIFIGSGAGLPRFMNIKGENLVG
                     IMSSNEYLTRVNLMDAANPESDTPVIKGKKVAVIGGGNTAMDAVRTARRLGAEKAMIV
                     YRRTEQEMPARVEEIKHAKEEGIEFMTLRNPIEYYGDKNGRVTHMLLQVMELGEPDAS
                     GRRSPVEVKGKTELVEVDEVIVSVGVSPNPLIPQSVKGLEVTGRGTLVVNEETMQTSI
                     PYIFAGGDIVRGGATVILAMGDGRKAAKGMNEWIRSFT"
     gene            13675..13863
                     /locus_tag="PLM04_04095"
     CDS             13675..13863
                     /locus_tag="PLM04_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HON02293.1"
                     /translation="MENEVSVKVAKGGPYIVSGNFKLVLPSGEEKEMSGKTALCRCGQ
                     SKNKPFCDGSHHDTEFDK"
     gene            14025..14831
                     /locus_tag="PLM04_04100"
     CDS             14025..14831
                     /locus_tag="PLM04_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON02294.1"
                     /translation="MDKLRFQSFGSGSSGNCYYIGNAEYGFLIDAGMGSRTIQNCLKN
                     IGLNFENIQGVFITHDHVDHIRSLWALERKFHLPVFSTRMISEAINQYYYREKNINLP
                     KRRYIEKNETLDIRDFSFTAFDISHDATDNVGYSIEYKGKRITFATDLGCIGKTAASY
                     LILSDYLVIEANYDEQMLRNGKYPDYLKKRISSSRGHLSNDQTGYFLSENYTEKMKNI
                     FLCHLSKENNSPQVALKTIRDYMENKNISVGKDVDVVALNRLNASELYEF"
     gene            14857..15606
                     /locus_tag="PLM04_04105"
     CDS             14857..15606
                     /locus_tag="PLM04_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HON02295.1"
                     /translation="MGENLVIIPTYNEKENIENIIYAVFRQPHDFHLLIVDDASPDGT
                     AEIVKNLQQKFPDRLFMIQRPGKMGLGTAYITGFKWALQHDYQYIYEMDADFSHNPDD
                     LPRLYASCALDGADMAIGSRYLTGVNVLNWPLKRVLMSYFASYYVRIVTGLKVTDTTA
                     GFVCYKRKVLETIDLDKIRFKGYGFQIEMKFTAEKCGFQLKEIPIVFINREYGTSKMS
                     SGIFGEAFFGVIQLKIESWFRDFPKKAIKSS"
     gene            15617..16972
                     /locus_tag="PLM04_04110"
     CDS             15617..16972
                     /locus_tag="PLM04_04110"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HON02296.1"
                     /translation="MSELQLIRNAQIVNEGKIFIGSVLINGELIESVHEGKNNYGEID
                     PNKVEIIEGDNLLLLPGVIDDQVHFRDPGLTHKGDIYSESKAAVAGGVTSFMDMPNTR
                     PPAVNNELLEEKFQLAAEKSLANFSFYIGATNDNLDELLKINPQQVCGIKVFMGSSTG
                     NMLVDDEKTLNAIFRDAPCLIATHCEDELTIRRNKEYYQSLYGENLPAHFHSLIRNED
                     ACFASSAKAVSLAKKYNSRLHILHLSTEKEMSLFDNSVSLEDKKITAEVCVHYLWFSN
                     KDYEQLGNKIKCNPSIKSETDKLSLIRAINNNTIDVIATDHAPHLLPEKEGNCLNAAS
                     GLPLVQHSLVTMLQLAKQGYFTIEKVVEKMCHAPARLFKIEKRGFIRPGYYADITLVH
                     PNDTWQVSKQNIFYKCGWSPFEEMSFDFRVLKTWVNGNKVFDEGKFDEQVRGKRLFFR
                     V"
     gene            17046..18173
                     /locus_tag="PLM04_04115"
     CDS             17046..18173
                     /locus_tag="PLM04_04115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HON02297.1"
                     /translation="MEFRDLKKQYKVLKNDIDQAMLEVSASGAFIMGKPVKEIEQQLA
                     EYVGVKHCVSCANGTDALTLALKTWGIKAGDAVFVPDFTFFASAEVVSLEGATPVFVD
                     VDADTFNISPASLERSIEKTIQQGKLRPKVIITVDLFGLPADYSAIRKIADKYHLLIL
                     EDGAQGFGGEINGKKACSFGDISTTSFFPAKPLGCYGDGGAVFTDNDEWASLMDSLRV
                     HGKGSFKYDNVRIGMNSRLDTLQAAILKVKFDAFRKYELNDINKVADFYTEKLKDLVK
                     TPFIPKGYLSSWAQYTIQLQDEKQRDGLQAYLKEQGIPTMVYYPKPMHEQTAYLDLGN
                     LSGDFSVTESLCRRVLSLPMHPYLSMEEINKVCQTILDYLK"
     gene            complement(18219..20858)
                     /locus_tag="PLM04_04120"
     CDS             complement(18219..20858)
                     /locus_tag="PLM04_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON02298.1"
                     /translation="MMGIRIEKISAENLGPLQRFNYEMKNVNLIYGKNEDGKSFLTEF
                     IIRSLFSGTRQWGELREAGNGRIIVSGFNGQEKTFSPRTKKNEKLDVLFSDENGLSPA
                     LAKLLIVKSGEINIAENNLNGIDLNTLKELLSSQKTLNTLAAKISPTIKNSKIDGNLI
                     TINRQGEGKIYYDELSNLNKIRHFIELVSSQSETGKIQELKEEFRLLKKKNEKLLLAR
                     RYKAWQYATEIENVHHQLENISESELSELETKIKNCRELQSQKNQLSIEIKTLEERTK
                     DFDETKNNFDRQIAAKQYAAYALNQKISALEKQIDAVDEKEISELEKNLELHNLYQTE
                     ISDKEPKLEELINQTQHFDWLEKVRTAYQQLQFSSSEKDTLNKILMVLSVVFAAGGLG
                     TLLALKNILFGAILFAGSFIALSFYVFRLQKTFKNKLRNSEWNQIKQEFQEKFHQELN
                     QVNLDTLYDALNKKIVELGYMQEDRKKTEQKILELRATIKEKTLGIFGQEVEMSKIYE
                     RLSSLKKQRNELNRHKEQLKQELMMLQVDVSDYSPNDPCVAYSKNLLDSLSKKMTELN
                     IWREQKGEKETRLRQLSELTTALKNEILESFRAIFGTEIPENKWEQNITERRQFQTQL
                     KEKLSGLTGELKGLGVSPQDYVQENQEIEFSAEEQTKLQNEMETLKEKISELENGIDK
                     LKSEICTCLNIDFTTGWNEVVENLYEREKETLSNLKKNEAYIVAANVLYKTIKQLQQQ
                     EDEELAAGINSGIFTNLLYRLTGKYNKLEIADNEIIISSENNSWLLKELSTGTKEQVM
                     LALRITLAQRALNETAFLMLDDAFQHSDYERRPKIIDQLFELSSAGWQIIYLTMDEHI
                     RDLFNQTAQHKQFLENYQEICLS"
     gene            complement(20868..21926)
                     /locus_tag="PLM04_04125"
     CDS             complement(20868..21926)
                     /locus_tag="PLM04_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HON02299.1"
                     /translation="MKIAITADVHLRTKAETPERYLALETLVNSMHDFDASTLVVAGD
                     LFDKDFHNYADFDAFCKLHSSKHFLVIPGNHDLLLEQKYFTASNLEIVSSPEIKILGS
                     IPFVFLPYDAKLSMDEALSNFFIQRKGEEELKNWVLIGHGDYVSRSREMNPYEKGIYM
                     PLSSGSVSAFRPSKVLLGHIHKPYTSGSVWYAGSPFPLDVNETGKRRWLLFDTDDNNL
                     VSVDLPASVIYFVENIVVLPSDNEISYLTQQLEQMIDNWQLLPEDFSKVKLRLSVKGF
                     TTDKAKISGFVNDFLHRKGIGFYDETGIDTTELNILKDEDRISLFEKVKKIIDELPNF
                     DYAGKDDIMEQTLKLIFV"
     gene            22209..23579
                     /gene="radA"
                     /locus_tag="PLM04_04130"
     CDS             22209..23579
                     /gene="radA"
                     /locus_tag="PLM04_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HON02300.1"
                     /translation="MAKTKTMYVCQNCGADSPKWIGRCPSCGEWNTYVEEVVSKGNAE
                     KIISGGFTVTAQKPVLLSEVEAIEEKRINTFNNELNRVLGGGLVPGSLVLIGGEPGIG
                     KSTLVLQVVLHLKGKRTLYISGEESVKQIKLRAERLKFDDPDCYLLSETSLEQIFVHI
                     QNIQPDLVIVDSIQTVSTEMLDASPGSVSQVRECAAAFLKFCKTTSIPVILIGHINKE
                     GGLAGPKVLEHIVDTVLQFEGDRHYMYRILRSIKNRFGSTSELGIYEMRQDGLREVEN
                     PSEMLFSQDHEGLSGIAIASAIEGIRPFLIETQALVSSAAYGMPQRSSTGFDLRRLNM
                     LLAVLEKRVGFKLNQKDVFLNIAGGLRVNDPAIDLAVISAILSSNVDIAIEKQVCTAG
                     EVGLSGEIRPIHRIEQRILEAEKLGFRKMIVPANNLKGLSLKNIEMEIIPVKKVEEAF
                     KILLKK"
     assembly_gap    23751..23850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23860..27459)
                     /locus_tag="PLM04_04135"
     CDS             complement(23860..27459)
                     /locus_tag="PLM04_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON02301.1"
                     /translation="MRQSGKISFFGFLLVFVLAGCFCEYFYQTSPQRVVHFNQFQSKL
                     ISKELSTARILKKMSNLLDNHSVNELINFSFPDDDISFYVYRGNELIFWSDHHLDVSS
                     ISLNDSVELHFVQLPNAYCVSKTLSADSLKLLALIKIKNNFPYENDQLINDFAKGFNL
                     NKNVGIVGGSNLDKYAVFSSHGDYLFSLSEPTVPVYDERWALAGLISFLLFFLLFFVW
                     YARGFSLSRTKSLQWKDFCGYVLITGALLAVMLYFNFPSVLLANKLFAPYLYAVPPLL
                     NSTVHLTLLTVYIFSTVFLFYRHVQLGNSVSFLRSFGLLLMLPLYFLLFYYVLRGVVY
                     HSGPQILILSFKNFSIFTVWMHILMLLWGIGFALLFFKIHTFLTRHYYIKIAFAIDAI
                     FSCLFAMAAFKVFPEMWKLSFFSYLFLWIPLYGFYIVNFSRKRHVKLIIYVLFFSIFF
                     IGNVVVLSNEKKFNKYRIAAENLSLNGTIENDQMTEILMEELDAQLSRDRFVNQLMRN
                     SDSVEVLSEYLNNSYLRGFWNRFEVHINAVSKNSDLCKEYQLFLQEKGIKIKQTHFYN
                     IIPNEKNISYLGVFSVLTAGNDSTCYFMEFYPRQNFRSYSFPNLLISSPPSILSQLNV
                     SLARYDHRKLVYRNGNFVYPDVSNWIPFENSNYFVVHYADRRHYVYAPDRNTYLVLTE
                     NVQYGEGAYLLYFCYAFLAFFALCWLLQQVDDFFAKGRKFTLSFINRFQISFIALLIL
                     SFIGIFYSSVDYIRNNYEKEQIQNLENKKIYIQKALQNLYYWNKDLSDKNTQTLNLDL
                     QDLSYTFHTDVHVYNNDGILVGSSQPLIFSKKLISNRIAPKPYFTEDANINQYENIGH
                     LNYLTAYTDFFNGDYLQIGYIAVPQFFSQDEIQNEIESFISVIVHIYFVIILLVIILT
                     LLIGNQISAPLMMLEKKLKEIRLGRRNEKIDYRYDDEIGQLVAQYNRTVDELEESARL
                     LARSERESAWKSMARQVAHEINNPLTPMKLTIQQLSRTKKMDKEKFNEYFDKASQMLV
                     EQIDNLSQIARAFSDFARMPEAKFERVELGELLKSEVEFFKHNYEKVDISFEGSKEPI
                     YTFADPKQLIQVFNNLIKNAIQSIPENRQGKIEVKISTSHQQIEIRISDNGAGIPEDI
                     RDKLFIPNFTTKSTGMGLGLAISKNIIEVTGGNISFTTQIDKGTTFIVTLPKA"
     gene            complement(27503..27976)
                     /gene="rlmH"
                     /locus_tag="PLM04_04140"
     CDS             complement(27503..27976)
                     /gene="rlmH"
                     /locus_tag="PLM04_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HON02302.1"
                     /translation="MKFVLINIGKTLDIHLSSLQEQYQNRLKHYLNFEVINIPELKNT
                     KNLSSSEQAAKESDLILKKLEIGDDVILLDERGTEFTSVEFADFLSKKMHSSNKRIVF
                     VTGGAYGFSEELYRRANNMVSLSRMTFSHQMVRLIFVEQLYRAMTILKGEPYHHA"
BASE COUNT         8791 a         5226 c         5139 g         8766 t
ORIGIN      
        1 aatctgctaa tgttagccga taagtatgca aaacaggtac atatttgcgg attgtgtcgc
       61 aaggttctgt ataatactgg ctaaatttat ctccatccaa gaccaaatat tgaatatagt
      121 atgagtttcc caatgatatt tcccaacttc tttctctaat tggacaatca agaataaatg
      181 aagagtcatt ttcaataaaa ccttcagaag ttttattgca attgaaaaca gtatcttcat
      241 agatgtcttg aatcttacta aatgcaaatt gataagcaac cctttttcct gatttattaa
      301 caaaaacaat attacggtgt acttcttcta tttccggaca agcacaaagc aacaaagtag
      361 ttgcaatcaa taatatggtt attctaaact gtttcataaa tttattttat ataaatattt
      421 agcaggtatg gactgatcaa aaataacttc aaatccagag ctgccaacta cgggcaatat
      481 catagacctt caagtagtat atctttatac tcattccttg taaacacatc tatccgttct
      541 gcaaatgcaa aacttacaaa atatacttct tcaggattat gaaatttata atttctactc
      601 aagtttcaaa gatagttgat tttttaaaaa taacaatgca atcacgacta atgcattaag
      661 cagaaggtca tccctgcgcg ttaatttcat ttcatatgct ccatcgaagc atgcttatgg
      721 tagagaataa tcatgcaatt attttacata gccccgtcgg ggcggtcatt tttttatcat
      781 aaaatttttg tcagatggtc attcctacgg aatttttttt gttcgttgcc tttttctacc
      841 aaaaggttgt ccttacggga caaaatcaat ttcacagtac cacacgaaag gttcgtacgg
      901 cagcgtggag atacgataca ttaccactat ttgttcatta tcttatgtga tgacaaatct
      961 gatacattca cttttgatta tctacagtat attggtggtg aataatctgt tccaataatt
     1021 aagtcaaact catccatata tttatttata aagtcatcaa tctcatattt tataaacttt
     1081 ttttctttgt tgtaaaacat tgaatataca tttagttgat tatttttaaa aaataaataa
     1141 tacccatttg tttgtccaat agtaaatatc aaatcagggt tctcttttaa aataatagac
     1201 gctaatttaa caccaaaatc agatttccca ataacctgat ctggataatt tgaatatgca
     1261 ccatcagcag aaccatagaa cacagaatct ttataaaagt aaatttcatc tatatataat
     1321 cgtttggtat ctaaataaca taatatattc cttatgtcat tcatcttctg aggataatgt
     1381 ttttttttga aaatactata aatcggctta gcaattacat tgctatcctg aagtttatag
     1441 ttaggatgaa ctttgtatcc tactttcata aactgtttta cagctttctt ttcattaatc
     1501 tttaagttaa aactctgttt ttgtatatct tcaacaaaga ttgaaataca ctcttgtgca
     1561 tagatttgca aaaaagaaaa ataaactaat gaaaaaatta tgacgattct tgttttcatc
     1621 ttttaatatt ttataaattc taacttaaga atttcttctg tcaatcttct tacagatgca
     1681 gaaagaggat cccaaacact tactgtattc atttttagaa atggcgtaaa accaccacca
     1741 aaccaattta acttatctgc cactttaaac gaaactacat ttactgcatg acctgccatt
     1801 tgatcagtac caattactcc tgccggggtg taaatataat cggtttgggt gatgtttcca
     1861 aatttatcga tgtctttttc gtacgtgcca aaataatagc ggatgttatt tttgctcggc
     1921 gaaatgtttg gttttatcaa cgattaaaat ctggagattt tttttactta cgacgtaggc
     1981 gggaggctac gccgaacaag agcggaacaa ccggctggcg atttttttag gaaggctaca
     2041 ggaaaatagt agcgctgggc ggcgtaactt ccggcaggcc ggttcaacgc taatactctg
     2101 accgttttat agctaatggg aaaatgctca ccaattaaag tttaaggatt tctttcaaaa
     2161 aaatgtagca gcaatataag actaatgccg gattacccag ctttttgtta tctgctattt
     2221 ctttgcatta tccagttata aaagtattca caagcttcat tttcgctact aaattctttt
     2281 atatataact tagtgcctct ttcaatataa taaacttccc atttattatt tttttcttcc
     2341 aggcagtaac tttccgtagg aagcaaaagc tcaaaatatt tagcatgctt aaattttagt
     2401 aaatcccatt tataatagat aaaagttttt ggatatttct cctttttaag ctgttttttt
     2461 aactctcttt ttaattgttt aatattcatt tacgggactc ttattatata accattttct
     2521 aacaggtaat tgatattatt atcaaacata tattgagtac ctcctcctga agaattgaac
     2581 caaaatgaag tacagtttga gggacttgcc aggtaaatcg agagataaat ttccacactt
     2641 tttctccaaa attcttgttc ggatctgttt taaataatcc catgtttatt ttccatgctt
     2701 tattcgtttt tgaccaagag gcagtaggat cgaaatcgct ccagacgttt ttgtctgacg
     2761 cttttgcttg ttaggtctaa tccaccttta aataaagcga ttaatacaaa gttcacaatc
     2821 ccagtaataa ttgaccagaa gaactcccca ctcgggtcag gtaaacattg ctatagtata
     2881 tttaacacaa taccttcacc taaagtttct gaaccctctc caaatgtaaa acgttgacca
     2941 atagttataa aatccagcaa caagtcaccg tgcgaaaaaa ttatataagc ttttatgttt
     3001 ttacttcctg aaatagtttt atcttggcct ataaactcta tttgaccaga attaaacata
     3061 tcttctctga ttttaaaagc aggtcgatac ccattaaagt aagttctatc tttcccaatt
     3121 atattgctgg aataaaacgt taaatctgct aatattctca ataattgcat aaactaaggt
     3181 ttattgatag tcattacatt atttacagac ggcaaatcaa cagtgctaat atagataaca
     3241 tcataaccat ccatgtggaa gtaattaaaa ttttgcgaaa tattataagg aacatctatt
     3301 tttataataa tagacttctg tccaaatgca gcatcatatg ctgaagtgtt atttacagca
     3361 tcttggtatg atttataaaa taacttacct tcttgataac cactaccatc tggattaggt
     3421 cttaatccat tactattaat gtttttatat tcgtcagttg aaacagctct gtatattgaa
     3481 attgtttttg gtgtagcctg tgtcaaattt tttcctgtcc atggatttac attattggca
     3541 taatttacgg caacagtaga agcaacgccc attgcgccgc caacgatcat gctcgatcct
     3601 aagccattaa aggaatttgc aaaagcatcg ctaagattct gaccggcaaa aaggttggcg
     3661 gtagttcttc ctgcaatgtg tccagcacct gcagcaagtg gtgaaacaac agcggatctt
     3721 aaaataggac tattgatacc atttactaac attgaagaat ttgcagccca atatccagca
     3781 gcaccacctg caaaaccaga cacacatcca atagcggaac taattccaac ctgtccccag
     3841 tcaacatttc ccattcctga aaaattatga cctgtttggg ctatgacact attagttccc
     3901 attattgctg cacttccggc cgccgaaact cctgcaatac ctacccatgt ggcacctgca
     3961 gcacctccag ttgcagcagt cgctgctcct gctccaaaag ccgcaacata ttgccaaaaa
     4021 ccttcacagt tgttccaatt tgcaattaga ttgactactc cgccgataag tgctccaaca
     4081 ataatatgcc aaaactcacc atccggatca gtatacttaa acgggttatt caggcaatag
     4141 ctgtaacggt tgaaactctg ggtgaagtcg ggtgcttgca cgtaattgtc agggctgagg
     4201 aagcgtgcca acacgggatc gcatactcgc ccgttcatgt tgatcagccc gaacggttcc
     4261 aaatgttcgt gaaacgtata gcctctgtcg gtgatatttg ctccgctgag caataccctg
     4321 ccaccccatg gggtgtaata ataactgctg acgatgtttt tattttcgtc tgttatggct
     4381 tgaatggagc ctaaattgtc ggtatgaaca tagtacaatg ttccattctt taccattgca
     4441 gttaatcctg ccggggtgta aatataatcg gtttgggtga aatttccaaa tttatctatg
     4501 tctttttcgt aagtgccaaa ataatagcgg atgttatttt tgctcggcga aatgtttggt
     4561 tttatgagcg gttaaaatca ggagattttg gcagtggtaa aatgcttaca ttagcgagtg
     4621 accaattgaa aaagaagctt gcaaaaaaag tttaatgatt tttttcagaa aaatgtagca
     4681 gcaaaacaaa agccggattg ctccggcttt ctcgttatgg ataggttact atccaatttt
     4741 tattttctaa ttcttcattt gttaatgttt ttttctttac aaacatttga tttttgtgta
     4801 tttcttttaa atcatagcta cgcatcgttt tttcagtaat aaaaaaaatc gttacttcat
     4861 tttcgttgct tggaagacgt ggtttttttc tatttccgcc aatagtatag gcatctttca
     4921 tattagcatc tataaaagta aataattcta atcctgaaat taacggcagt gaatccgcat
     4981 ttccatattt gaggtaaacg taaacagcct cattagtatt attatgaaca tagagtaatc
     5041 cacgtgcagg gtcgtaggta aaaatgcaac cgtgaaacaa aaataaaata ctgaaataaa
     5101 ttaacttttt catgctcttg aaaataataa tattatggcc aaaaaaatgg tgatactgca
     5161 cgcaaacgca ataaattaaa ccaacttata ttttgtgctg gccatgcaga attatttaat
     5221 aatgaattaa caccaaagtg gttatttgat aaataattag accatgtttc agtccaaaaa
     5281 gtactgtgac ttcagccacc tactccatcc gttctgcaac tgcaaaactt acaaaatata
     5341 cttcttcagg attatgaaac ttataatttc tactcaagtt tcaaagatag ttgattttta
     5401 aaaaaataac aatgcaatca cgaccaatgc attaagcaga aggtcatccc tgcgcgttaa
     5461 tttcatttca tatgctccat cgaagcatgc ttatggtaga gaataatcat gcaattattt
     5521 tacatagccc cgtcggggcg gtcatttttt tatcataaaa tttttgtcag atggccattc
     5581 ctacagaatt tttttgttcg ttgccttttt ctaccaaaag gttgtcccta cgtgacaaaa
     5641 tcaatttcac agtaccacac gaaaggttcg tgcggcagcg tgaaaaaatt caaaactggt
     5701 tagacgttgc atgcaacatc tctactttta tttcttattt cgtaattcaa agctcagaac
     5761 tctggactca aaactcgaaa ctatattttt ttggtaaaat gaaatctaaa tttttccttt
     5821 caaaaacttt ccggtatagg aatttccgca atttacaatt gcttcgggag ttccttcaaa
     5881 aaccacataa cctcctttgt cgccgccttc gggccctaaa tcgataatgt gatcggcaca
     5941 tttgattact tcgggattgt gttcgataat gagaacggta tggcctttgg caatcagcgc
     6001 atcaaaagct ttgagtaaag ttttgatgtc gtggaaatgc agtccggttg taggttcatc
     6061 gaaaataaag agtgttgggt ccgatttttc gtttgccaga aaagacgcca attttacccg
     6121 ctgactttcg ccgcccgata aggtattgga tgattgtccc aatttgacat atccgatacc
     6181 gacatcctgc aatgacttga gacgtttcac aattttcttt tccaacgtat tgtttgattg
     6241 tccaaaaaat tcgatagcct gattcacagt catttccagc acatcataga tattcattcc
     6301 tcgataggta acttcgagaa tatcctgttt aaaacgtttc ccgtgacaat gttcacattc
     6361 cagtaccaaa tcggccataa attgcatttc gattgtgatg gttccttctc cctgacattc
     6421 ttcgcaacga ccgccttccg tattgaaaga gaaaaaggaa ggattataac ccatttgttt
     6481 ggccaatggt tgttcggaaa aaagttttct tatgtcgtca tacactttta tataactcac
     6541 cggattggag cgagaagaac gtccaatggg attttgatcg acaaattcta cctgactgat
     6601 cagttgcaaa ctgccttgca aaacaccaaa ctgtccggta tgatcaacca ctccaccata
     6661 atattttttc aaagcagcat aaaatacatc ctgaaccagt gacgattttc ccgaaccgct
     6721 gacaccggtt accactgtca tcacattcag aggaaatttt actgaaatgt tttttaaatt
     6781 gttttccgta gctccgacaa tttctatgta attgttccat ttgcggcgaa gggtaggaac
     6841 ggggatttct tccgtatgat acaaatattt caatgtgtaa gaattggcaa tagtttcctt
     6901 cgtattttcc tgtttgtcaa tatcgttcag ttgaccattg aagacaatat tcccgcccaa
     6961 tctgccggct ccgggaccaa tatcgataag gtaatctgcc gaacgaataa tttcttcatc
     7021 atgttctacc acaataactg tatttcccaa attacgcagc tgatgcaata cgttgatcaa
     7081 cttttcggtg tcgcgcggat gaagtccaat actcggttcg tccaaaatat acagcgaccc
     7141 caccaagctg cttcccaaag aagttgccag attaattcgc tgactttcac cacccgaaag
     7201 tgtattggaa agtcgattca atgtaagata acccaatcct acatcaagca aaaaacggat
     7261 acgattttga atttctgtca acaaacgact ggcgatggct gcgtcgtgtc cgtcaagcga
     7321 aagttgttca aaaaaatcgt ataattcttc caccggcatc aacaccaatt ccgtaatggg
     7381 ttttccaccg acgcgcacat aagttgcttc ctttttaagt cgagttccct tgcaagccgg
     7441 acaggtggtt tttccccgat accttgccag catcacgcga tattgaattt tgtactgatt
     7501 ttcttccaga aacttgaaaa agctgttcaa tccttcaaaa tattcattcc ctgtccaaag
     7561 aagttgtcgt tgactttcag taagttcgta ataaggttta tgaatgggga aaccaaattt
     7621 tggtgcagcc tgaattaacc tttttttcca ttcactcatt acttcgcctc tccagcatac
     7681 aatggcgtct tcgtaaacgg aaagcgattt gttgggaaca acaagatctt cgtcaatgcc
     7741 gataattttt ccaaagcctt cacacaccgg acatgcacca atgggactgt tgaagctgaa
     7801 agtgtgttcg gtaggaattt caaatgtaat gccgtcggct tcaaaatttt ttgaaaaatg
     7861 tagcagtttt tctccttctt ccgtattcac tttcagaata cagtttccat tgccttcgta
     7921 aaaagcagtt tccaccgaat ccgtcaaacg gcttatggtg gatggggaat gatcaaccac
     7981 caatcggtcg ataaccaaat atatttcagt gtttttttcc agcaattcgg ggcgaagcag
     8041 caaatcctga attctgagca tttcgttgcc cgcttccact cgactgaatc cctgcatttg
     8101 gaaatttcga agcttttcag tcaaattgtt ttcttcattc aaccgaatgg gaatgagtaa
     8161 agtaagtttg gtatcgtcgg gatacgacat ggctgcttcc accacatctt ctgcctcgtg
     8221 aatttttacc aattctcccg aaattggcga atacgttttt ccaatgcgcg cataaagcaa
     8281 tttcatgtac tcgtaaattt cggttgaagt tccaaccgtt gatcgcggat tgcgagtgtt
     8341 gaccttttgt tcgatggcaa tagccggcgg tatgcctttt atgtaatcga cttcaggttt
     8401 gctcattcgt cctaaaaatt gtcgtgcata agccgaaaga ctttccacgt atctgcgttg
     8461 tccttcggca tatatggtat cgaaagctaa agatgatttt cccgatcccg ataagccggt
     8521 aactaccacc aatttgtttc tcggaatttc aacatcaatg tttttaagat tgtggacacg
     8581 tgcgccttta acaataatat ttccttgttt ggacattcaa ctgctcccta aaaattaaaa
     8641 taaacaatag aaaatcagat tgttatttaa aaatttttct gattattctt cagtaaacga
     8701 cgatttttaa ataacctgca aagatacaat ttattgagcg gatgagtaat agatgcagat
     8761 aaaatttatt gaatatagtt attagctgag tcatatggaa ttttattgtt ttaattttcg
     8821 aatgagaaac aaaacactga aattgtttca tatagccaat tgattgagtg aattattaat
     8881 aaaaaataaa agattatttt ttaaaaatta aaatattctg tacatttgct caattaaacg
     8941 attcagtata aaataaaaac taaacgtatg aaattcatag tttccagcac agaacttttg
     9001 agccatttgc aaacgattag cagggttatc aacaccaaga atactttgcc tattctggat
     9061 gatttcaaat tttctctcga aggcaatact ctgacgatga ctgcttccga tgttgaaaca
     9121 accctcatca cttccataca ggtggaggaa tctgaaggag aaggtcaagt agctattgct
     9181 tctcgtttgt tactggatac actgcgcgag ttttcggaac agccgctgac tttttccatt
     9241 aacgaatcca atttgagcct caccatcacc acttccaatg gagcttataa ttttattggt
     9301 gagaatgcgg atgaatttcc tcgtttgcct ccatttgaag aagaaccttg ttcttttgaa
     9361 ctccctgttg acaacttgct ttcgggaatt tccaaaactt tgtttgcagc tgctgccgat
     9421 gatttgcgtg aagtcatgaa tggtatttat tttgatttat ctcctaaaga actcaccatt
     9481 gttgcaaccg atgcacacaa actttcgcgg tataaaacca caaatgtgag tacttctttg
     9541 gatgaagatg aagtaaaaag ttttgtactt cccaaaaaac cggcaaacct cttgaaaaac
     9601 atcctgccaa gagaaaaggg taacgtaaaa attaggttcg acaagaagta catccatttt
     9661 gaactggaaa actatacgat ggtttgcctg caggtggccg ggaaatatcc caactataac
     9721 agtgtcatcc ctttcaacaa tcccattaaa attattgttg accggctaat gttgcttaat
     9781 tccgtcagaa gggtttccgt attttgcaac caggcaacca accttataaa actggttttc
     9841 aacgaacagc aggtaaatat ttcggccgaa gacattgatt tttctatttc tgccgaagaa
     9901 accattcctt gtcaattgga aggaaatgct atcactatcg gattcaaagc tacttatctt
     9961 atcgacattc tttccaatat cgattcttct gacgttgtaa tcgagctttc cgatccaagt
    10021 cgcgctgcat tgatttcccc cctcgaaaac gaagaaaacg aagaagtctt aatgctcctt
    10081 atgccgatgc ttttaatgga ttacgattaa attcacgatt ttttcagtcg gattattttt
    10141 aaggtctaag gtgaaaaaac tttagacctt gttttttcga ttttagcttt gttcaatttt
    10201 tccaaaaaaa aaatttcatg gaattaaatt taaaaaaacc gattgttttt ttcgacctcg
    10261 aaacaacagg tatcaacatt gttaccgacc gtattgtgga aatttcctat cataaggttt
    10321 tccctaacgg aaaagaactt acgaagacta aacgtattaa tcccgggatc cctattcctg
    10381 cctatgcaac tgctgttcat ggcattaccg atgccgatgt ggccaattgt cctactttta
    10441 aggaagtagc caaagaaata gcggccgatt ttgaaggaag cgatttggca ggttacaact
    10501 ccaatcggtt cgacattcca cttttggcag aagaattttt gcgtgcagaa gtagatatag
    10561 atttgatgaa acaccgattt atagatgtac aggttatttt tcacaaaatg gagcagcgga
    10621 ctttgtcggc ggcttacaaa ttttactgca acaaggattt gaatgatgcc cacagtgccg
    10681 aagccgatgc aatggctacc tatgaagtgc tgaaagctca attggaacat tatcccgaat
    10741 tgaaaaacga tgtagatttt ttatccgatt ttactaccca gaacaacaat gtggactttg
    10801 ccggacgcgt catttacaac gaaaaaggag aagaaattat caatttcggt aaatacaagg
    10861 gcaaaaaggt ggaagatgta cttaaaaccg atatgggata ctatggttgg attatgaaca
    10921 gcgatttttc actccatacc aaaaaagttt ttaccaacat taaacttcgc caattcaaca
    10981 acaaataatt gcataaagtt aatttggatt gcaaaaaatc ttcttatatt caggaaaata
    11041 ttcaaaaatt taatggttcc cgatacttgg tgcaaattga tgttaattgc atatctgccc
    11101 gtttttcaga aaacgacttc ctgatattaa atctgctcgt tgtcaactaa agcatttcat
    11161 ctttttgtgt gaaaattgaa aattatcgtg taattttgtc ttttgaaact aaaacagcca
    11221 aaaatgaata aaattgtcaa caaagaatat ttgtccccca acgtggtgaa gtttgaagtg
    11281 gaagctccat tgatagccaa agctcgcaaa gcaggccatt ttgtgattgt acgggttgga
    11341 gaaaaaggag aacgcattcc cttaaccatt gccgatgcca atacagaaaa aggaaccatt
    11401 actttgattg ttcaaaaaat tggtttgtcc acttccaaac tttgcgaatt gaacgaaggc
    11461 gattgcataa ccgatttggt aggtccgctg ggaaaagcga cccatattga taattttgga
    11521 acggtagttt gcgcttgtgg aggtgtagga acagctccga tgcttcccat agccgaagcc
    11581 atgaaaaaag cgggtaatca cgtaataaca gttttggccg cacgtacaaa ggaattaatc
    11641 attttggagg aacaaatgag aaaattttcc gatgaagtaa tcattatgac cgatgatggc
    11701 tcttatggca acaaaggttt ggtaaccgat ggtgttgaaa tggttataaa gcgccaaaaa
    11761 gtagatttat gtgtaactat cgggccggca atcatgatga aatttgtgag tgaactgaca
    11821 aaaagatata atattcctac tattgcttcg ctcaatacca tcatggtaga tggaacaggc
    11881 atgtgtggcg cttgccgcat ttccgttgga ggaaaaacaa aattcgtttg cgtggatggc
    11941 cctgaatttg atgctcatca ggttgatttt gatgaaatgc tgatacgctt aaacggatac
    12001 aaggatatcg aaagagaaca attacacaaa atatctccta acaattgatt ccccagcaaa
    12061 atatttttgt tgtagaaaca aattactttt ttgaaaattt tttaagtccg aagaaatgac
    12121 aaattttaat gaggaaagaa tgcaaccttg gcgggtggaa ctgagaaatt ccatgaagcc
    12181 gaaagacaga atggcaattg ccagagtaaa aatgcccgaa ctcgatcccg aatatcgcag
    12241 ccacaccatg aaagaagaag tcaatttggg tttaacgccc gaaatggctt tgatggaatc
    12301 gaagcgatgc attgattgtg ccaacccaac ttgtgttcaa ggttgccctg tggctatcaa
    12361 tataccgaaa ttcatcaaaa atattgaacg aggagaattt ttggaagctg ccaaaacatt
    12421 aaaagaaact tcagtattgc cggcagtttg cggacgagtt tgccctcagg aaaaacagtg
    12481 tgaagcacag tgcattcatt taaaaacaaa aaacgaagcg gttgccattg gttatctcga
    12541 acgttttgct gccgattacg agcgtgaaac aggcaacata tccattcccg aaatagccgc
    12601 tccgcttcac aaaaaagttg ctgctgtagg ttcgggccct gccgggttgg cttttgccgg
    12661 cgacatggca aaaatgggtt acgaagtggt gctttttgaa gcattgcatg aaattggagg
    12721 cgttcttcgg tatggaatac ctgaatttcg tttgcccaac aaaattgtag atgccgaaat
    12781 taggaatctg attgctatgg gtgtacattt tgtgaccaac tgcattattg gcaaaaccat
    12841 tactatagac gacttactgg ctgaagatta taaaggtatt ttcattggaa gtggagccgg
    12901 attgcctcgt ttcatgaata taaaaggcga aaatctggtt ggcatcatgt catcaaacga
    12961 atacttgaca cgtgtcaatt tgatggatgc agccaatcct gaatcggata cacctgttat
    13021 aaaagggaaa aaggtggccg taattggtgg tggaaatacg gcaatggacg cagtaagaac
    13081 cgccagacgt cttggcgcag aaaaagccat gatcgtttat cgccgaacag agcaggaaat
    13141 gcctgcccgt gttgaagaaa tcaaacatgc caaagaagaa ggaattgagt ttatgacttt
    13201 acgaaatccc attgaatact atggcgataa aaacggaaga gttacccaca tgctgcttca
    13261 ggttatggaa ctgggcgaac ctgacgcttc gggacgccgc tctccggtag aagtcaaggg
    13321 aaaaaccgaa ctggtggaag ttgatgaagt aattgtcagc gtaggagttt cacccaatcc
    13381 gctcattcca caatcggtaa agggactgga agttaccggc agaggaacat tggtagtgaa
    13441 cgaagaaacc atgcagacga gcattcccta tatttttgcc ggcggagata ttgttcgtgg
    13501 tggtgctacg gttattcttg ctatgggcga cggaagaaag gctgcaaaag ggatgaatga
    13561 atggattagg agctttactt gaaatttgaa taagatttaa aaaatatttt ttaaaaaact
    13621 ttttcctatt aattttgttt ccatttacaa agttaaattt aaaatcttac taaaatggaa
    13681 aatgaagttt cagtgaaagt agccaaagga ggcccttaca tcgttagtgg taatttcaag
    13741 cttgttttac cttcaggaga agaaaaagaa atgagtggta aaacagcact ttgtagatgt
    13801 ggacaaagca aaaataaacc tttttgtgat ggttcacatc atgatacaga gttcgataaa
    13861 taattgaaga attgttgatt ttattaaagg aaacagggat aaaaaattgt ctcctgtttc
    13921 ttttttttct ttctgaggat gttgaaacat taactttatt tccgaataat tttttggtgc
    13981 aatttccgat taatgttaat tttgttcttt ctaaaattta ttgaatggac aaattacgat
    14041 ttcaaagttt tggtagtggc agtagtggaa attgttatta cattggaaat gccgaatatg
    14101 gttttttaat agatgcaggc atgggatcgc gaactatcca gaattgcctg aagaatatag
    14161 gtctgaattt cgaaaatatt cagggcgtgt ttattactca tgaccacgtc gatcatatac
    14221 gttcattgtg ggcattggaa agaaaatttc atcttccggt tttttccacc cgaatgattt
    14281 cggaggcaat caatcaatat tattatcgtg aaaagaatat taatttgcct aaacgaagat
    14341 atatcgagaa aaatgaaaca cttgatataa gggatttttc ctttaccgcc tttgacattt
    14401 ctcatgatgc tacggataat gttggttaca gcattgaata taaagggaaa agaattacat
    14461 ttgcaacaga tttgggttgt attggaaaaa cagccgcatc ctatttgatt ttgtccgatt
    14521 atttagttat cgaagccaat tatgatgagc aaatgctccg aaatggaaaa tatccagatt
    14581 atttgaaaaa aagaatttct tcttccagag ggcatttgag caacgatcag accggttatt
    14641 ttctttctga aaactataca gagaaaatga aaaatatatt tctttgtcat ctcagcaagg
    14701 aaaacaactc tccacaagtt gctctgaaaa cgatcagaga ttatatggaa aataaaaata
    14761 tttcggtagg caaggatgtg gacgtggtag cgctgaaccg actaaatgct tccgaattgt
    14821 acgaatttta aatttattcc tctgcaaatt aaaactatgg gtgaaaattt agttattatt
    14881 cctacttaca acgaaaagga aaacatagaa aatattattt atgctgtttt tcgtcagccc
    14941 catgatttcc atttgttgat tgttgatgat gcttcccccg atggaacagc agagatagtg
    15001 aaaaacttgc aacaaaaatt ccctgatcga ttgtttatga ttcagcgccc gggaaaaatg
    15061 ggactcggaa ctgcttatat taccggcttt aaatgggctt tgcagcacga ttatcagtat
    15121 atttacgaga tggatgccga tttttctcat aatcccgacg atttaccccg attatacgct
    15181 tcttgcgctc tcgacggagc agatatggct attggttcac gatatctcac aggcgtgaat
    15241 gtattaaatt ggccgctaaa gagagtttta atgtcttatt ttgcttccta ttatgttcgt
    15301 atcgttaccg gtcttaaagt taccgatact acagcaggat ttgtttgtta taaaagaaaa
    15361 gtgctggaaa ccattgatct ggataaaatt cgttttaaag gttatgggtt tcagatagaa
    15421 atgaaattta cggctgaaaa atgcggattc caattaaaag aaattccaat tgtttttatt
    15481 aatcgggagt acggcacttc aaaaatgagc agtggtattt tcggagaagc tttttttggt
    15541 gtaatccaat tgaaaattga aagctggttc agggatttcc ctaaaaaagc aatcaaatcc
    15601 agttaaatta tttattatga gtgaactgca attgattaga aatgcacaaa tagttaacga
    15661 aggaaaaata ttcatcggtt ctgtgctcat aaatggagaa ctaattgagt cagttcacga
    15721 aggaaagaat aattatggag agatagaccc aaataaggtt gaaatcattg agggagacaa
    15781 tttacttctt ttgcccggcg ttatagatga tcaggttcat tttcgcgacc ccggacttac
    15841 tcataaaggt gatatttatt ctgaaagtaa agcagctgta gctggaggag ttaccagttt
    15901 tatggatatg cccaatacgc gtcctcctgc tgtaaataat gaattgctcg aagaaaaatt
    15961 tcaactggca gccgaaaaat cgcttgccaa tttttccttt tatattggag ctaccaatga
    16021 caatttggat gaattactga aaataaatcc tcaacaggtt tgtggtataa aagtttttat
    16081 gggttcttct acagggaata tgttggtaga cgacgaaaaa acattaaatg ccattttccg
    16141 tgatgccccc tgtctgattg caacacattg tgaagatgaa ttaactatcc gcagaaacaa
    16201 ggaatattat caatccttgt atggagaaaa tttacccgcc catttccatt cgctcattcg
    16261 taatgaagat gcttgttttg cttcttcggc gaaagcagtt tcgttggcaa agaaatataa
    16321 ttctagattg cacattttac atctttcaac cgaaaaggaa atgtctttgt ttgataactc
    16381 tgtttcactt gaagacaaaa aaataacggc tgaagtttgc gttcactatt tgtggttttc
    16441 taataaagat tatgaacagt tggggaacaa aattaaatgt aacccttcca taaaatcaga
    16501 aaccgacaaa ctttcactta tacgggcgat aaacaataat accattgatg taattgctac
    16561 tgatcatgct ccacatttac ttcccgaaaa ggaaggtaac tgtttgaacg cagcttccgg
    16621 attgcctttg gttcagcatt cattggtaac catgttgcaa ttggcaaaac agggttattt
    16681 taccattgaa aaagtagtcg aaaaaatgtg tcatgcgcca gcccgattat ttaaaataga
    16741 gaaaagaggg tttattcgtc cgggatatta cgcagatata acattagtac atcctaacga
    16801 tacttggcag gtttccaaac aaaatatttt ttataaatgc gggtggtcgc catttgaaga
    16861 aatgagtttc gactttcggg tactgaaaac atgggtcaat ggaaataaag tatttgatga
    16921 aggaaaattt gatgagcaag tgaggggaaa acgattgttt tttcgagtat aaacaattct
    16981 tttcatcaga atataattag ttaattatag agcaaataaa atttgaaacc acaaataata
    17041 aaactatgga attcagagat ttaaaaaagc agtacaaagt tctgaagaat gatattgatc
    17101 aggccatgtt ggaagttagt gcttcgggcg catttatcat gggaaaaccc gtcaaagaaa
    17161 tagaacagca attggccgaa tatgttggcg taaaacactg tgtttcgtgt gcaaacggaa
    17221 cagatgcctt gacattggct ttaaaaacat ggggaataaa agccggagat gcagtttttg
    17281 tacctgattt cacttttttt gcttcagccg aagttgtttc tctcgaagga gctacacctg
    17341 tttttgttga cgtggatgcc gatactttca atatttctcc tgcatctttg gaacgaagca
    17401 tagaaaaaac cattcaacag ggaaaacttc ggcctaaagt cattattacg gtcgatttgt
    17461 ttggactacc cgccgattat tctgctatta gaaaaattgc cgacaaatat catctgttga
    17521 ttttggaaga tggagcacaa ggtttcggag gagaaattaa cggcaaaaaa gcttgcagtt
    17581 ttggcgatat ttccactact tcgtttttcc cggctaaacc cttaggttgt tatggtgatg
    17641 gcggagcagt ttttaccgat aacgacgaat gggcttcgct gatggattcc ttgcgggtac
    17701 atggcaaagg ttcttttaaa tatgacaatg tgcgtatagg tatgaactca cgtctcgata
    17761 cattgcaggc tgccatttta aaagtgaaat ttgatgcttt cagaaaatat gaattgaacg
    17821 acatcaataa agtagccgat ttttatacgg aaaaattaaa agatttagtg aaaacgcctt
    17881 tcattcccaa aggatatctt tccagttggg cacaatacac cattcaattg caggatgaaa
    17941 agcagcgaga tggcttgcag gcttatttga aggaacaagg tattcccaca atggtgtatt
    18001 atccaaaacc catgcatgag caaacagcct atcttgattt gggcaattta tcaggtgatt
    18061 tctccgtaac cgaatcccta tgccgcagag tactttctct accaatgcat ccttacttga
    18121 gtatggaaga aattaataaa gtctgccaaa cgattttaga ttatctcaaa taaatttcaa
    18181 ctgttatctc tgataagact ttaataattt tatagcattt acgacaaaca gatttcctga
    18241 taattttcaa gaaattgctt gtgttgagct gtttggttaa aaagatcacg aatatgttca
    18301 tccattgtca gataaatgat ttgccagccg gcagatgaca attcaaacag ctgatctata
    18361 attttggggc gtcgctcgta atcgctatgc tgaaaagcat cgtccaacat cagaaaagcc
    18421 gtttcgttga gtgctctttg tgccaaagtt atgcgaagag caagcattac ctgttctttg
    18481 gttccggtgc tgagttcttt tagcagccac gaattatttt cactcgatat gattatttca
    18541 ttatcggcaa tttccagctt attgtatttt cccgtaagcc gataaagcaa attggtaaaa
    18601 atgccggaat tgataccggc agccaattct tcatcttcct gctgttgaag ttgtttgatt
    18661 gttttgtaaa gtacatttgc ggccacaatg tacgcttcgt tttttttaag attagagaga
    18721 gtttcctttt ctctttcgta aagattttca accacttcgt tccatcctgt ggtaaaatca
    18781 atatttaaac acgtgcaaat ttccgatttc aacttgtcga ttccattttc gagttcggaa
    18841 attttttctt ttaaagtttc catttcgttt tgaagtttag tctgttcttc cgccgaaaat
    18901 tcaatttcct gattttcctg cacataatct tgaggcgaaa caccgagtcc cttcaactct
    18961 cccgttaggc ccgaaagttt ttccttcagt tgcgtttgaa actgacgtcg ttccgtaata
    19021 ttttgctccc atttgttttc tggaatctct gttccaaata tggcccgaaa gctttccaaa
    19081 atctcatttt tcaaagctgt agtcagttcg gaaagttgtc gaagccttgt ttctttttct
    19141 cctttttgct cacgccagat attaagctca gtcattttct ttgaaagtga gtccaataaa
    19201 tttttgctgt atgcaacaca gggatcattg ggagaataat cggaaacatc cacctgcaac
    19261 atcatcagtt cctgcttcag ttgttctttg tggcgattca actcgttgcg ttgttttttt
    19321 aatgaagata gccgctcgta aattttcgac atttctactt cctgcccaaa aataccaagc
    19381 gttttttctt ttattgtggc tctcagttcc aaaattttct gttcggtttt ttttcggtct
    19441 tcctgcatat agccaagttc aacaattttt ttattgagcg cgtcataaag agtgtccaga
    19501 tttacctgat tcagttcctg atgaaatttt tcctgaaatt cttgcttgat ctgattccat
    19561 tccgaatttc ggagtttatt tttgaaagtt ttttgaagac gaaaaacgta aaatgacaac
    19621 gctataaatg aaccggcaaa cagtattgcg ccaaaaagaa tgtttttcaa cgctaaaagt
    19681 gttcccaaac cgcctgctgc aaagacaaca gataaaacca tcaatatttt gttgagcgta
    19741 tctttttctg acgacgaaaa ttgaagttgt tgataagccg tgcggacttt ttccaaccag
    19801 tcgaaatgtt gggtttgatt tattagctct tcaagctttg gttctttgtc agatatttcg
    19861 gtttgataaa gattgtgtaa ctcaaggttt ttttcgagtt cagaaatttc tttttcatca
    19921 acggcatcga tttgcttttc caaagcggaa attttttggt tcagcgcgta agctgcatat
    19981 tgttttgctg caatttgccg atcgaaattg tttttcgttt catcaaaatc ttttgtccgt
    20041 tcttcgagcg tttttatttc gatactcaac tgattttttt gggattgaag ttctctacag
    20101 tttttaattt ttgtttccag ttcggaaagt tcggattcgg aaatattttc caattgatga
    20161 tgaacatttt caatttcagt ggcatattgc catgccttgt agcgacgagc caacaacagt
    20221 ttttcgtttt tcttctttaa aagacgaaat tcttctttca gttcctgaat ttttcccgtt
    20281 tcagattggc tggagaccaa ctcgataaag tgccggattt tattgagatt acttagctcg
    20341 tcgtaataaa ttttgccttc accctgtcga ttaatggtaa tgagattccc gtcaattttt
    20401 gaatttttta tggtaggaga tatctttgct gccagtgtat tcaatgtttt ttgtgaagaa
    20461 agcagttctt tcagggtatt taagtcgata ccgttcaaat tgttctcggc gatgtttatt
    20521 tctccacttt tgacaatcaa taatttggcc aatgccggcg aaagcccgtt ctcatcagaa
    20581 aaaagcacat ccagtttctc gttcttttta gttcggggtg aaaaagtttt ttcctgtccg
    20641 ttaaaacccg aaacaataat tctgccattg ccggcttcgc gcaattctcc ccactgacga
    20701 gtgccggaaa aaagcgaccg aatgataaat tcggtcagaa aagattttcc gtcttcattt
    20761 tttccataaa tcaagttaac atttttcatt tcataattga aacgttggag cgggcctaaa
    20821 ttttcagcag atattttttc gattcggatt cccatcatta cttgtgatta aacaaaaatc
    20881 aattttaaag tttgttccat aatatcgtct ttgccggcat agtcgaagtt tggcaattcg
    20941 tcaatgattt ttttaacttt ttcgaacagc gaaattctat cttcatcttt taaaatgttc
    21001 agttcggtgg tgtcgattcc tgtttcatca taaaaaccga tccctttccg atgcaaaaaa
    21061 tcgtttacaa aaccggaaat ttttgcttta tcggttgtaa aacccttaac cgatagtcgc
    21121 aatttgactt ttgaaaaatc ttccggcaac agttgccaat tgtctatcat ttgttccagt
    21181 tgctgggtta aataggatat ttcgttatcg gaaggcaaca caacaatatt ttcgacaaaa
    21241 taaatgaccg aagcgggtaa atcgaccgaa accagattat tgtcatcggt gtcgaaaagc
    21301 agccatcttc tttttcccgt ttcgtttacg tcgaggggaa aaggagaccc tgcataccaa
    21361 acactgcccg atgtgtaagg tttgtgtata tgtcccagta gaacttttga gggtcggaaa
    21421 gcagaaacgc tgcccgaaga caagggcatg taaattcctt tttcgtaggg attcatctcg
    21481 cgacttctgg aaacataatc accatgacca ataagcaccc aattttttaa ttcttcttct
    21541 ccttttcttt gaataaaaaa attactcaag gcctcatcca ttgaaagttt ggcatcataa
    21601 ggcaaaaaaa caaaaggtat ggaacccaaa atttttattt cgggagaaga tacaatttcc
    21661 aagttggaag ccgtaaaata tttttgttcc aacagtaagt cgtgattgcc aggaatgacc
    21721 agaaaatgtt tggacgaatg aagtttacaa aaagcatcaa aatcggcata attatgaaaa
    21781 tccttgtcga aaaggtctcc ggcaaccaca agtgtggagg catcgaaatc gtgcatggaa
    21841 tttacaagag tttccaaagc caggtatcgc tcaggcgttt cggctttggt tcgcaaatgc
    21901 acatcggcag taatggctat tttcattata cgtttctgac aaaaagtgta acgactaaag
    21961 gaaaaaaatc agtaaaagat tcactaaaaa attgttttcc aaaaacataa gttattcgtt
    22021 aggtaaacaa ttttagcaca taaaattagc aattttattc acattttcaa aaaaacttgc
    22081 agttaaactc aaatataatt tccagaaaaa acgtaaagaa acatgtaaat ggaacagaaa
    22141 aaatctctaa ttttgtaact ttcagcaata aaaaattgga cgctcaaaag aaaaaaatat
    22201 caaaagtcat ggcaaaaacc aaaactatgt atgtctgtca gaattgtgga gccgattcac
    22261 ccaaatggat aggtcgatgt ccttcctgtg gcgaatggaa tacttatgtg gaagaagtgg
    22321 tgagcaaagg aaatgcggaa aaaatcatca gtggaggttt taccgttact gctcaaaaac
    22381 ccgttttgtt gagcgaagtt gaagctattg aagaaaaacg catcaacact ttcaacaatg
    22441 aactgaaccg tgttttggga ggaggattgg taccgggatc gcttgtattg attggcgggg
    22501 aaccgggaat tggaaaatcg acactggttt tgcaagtggt tttgcatctc aaaggaaagc
    22561 gaacgctata tatttcggga gaagaaagcg taaaacagat taaattgcgg gcggaacggt
    22621 tgaaatttga tgatcccgat tgttatcttt tgagtgaaac ctcactggaa caaatttttg
    22681 ttcatattca aaatatacaa cccgatttgg taattgtgga ttccattcag accgtttcta
    22741 ccgaaatgct ggatgcttct cccggttcgg tatcgcaggt tagagaatgt gcagccgctt
    22801 ttctgaaatt ttgcaaaacc acttccattc cggtaatttt gattggacac atcaacaagg
    22861 aaggcggtct ggcaggtcca aaagtactgg aacacattgt ggacaccgtt ctgcaattcg
    22921 aaggcgaccg acattacatg taccgtattt tgcgttcgat aaaaaaccga tttggcagta
    22981 catccgaact cggcatttac gaaatgcgtc aagatggttt gcgcgaagtt gaaaatcctt
    23041 cagaaatgct cttttcgcag gatcacgaag gattgagcgg cattgccatt gcttcagcca
    23101 ttgaaggcat tcgtcccttt cttatcgaaa cgcaggcgtt ggtaagttcg gctgcttacg
    23161 gaatgccaca acgctcgagt acgggttttg accttcgtcg attgaatatg ttgctggcag
    23221 tgctcgaaaa gcgtgtgggt tttaaattga atcagaaaga tgtatttctc aatattgccg
    23281 gcggattacg agtaaatgat ccggctattg atttggctgt gatttctgca attttgtctt
    23341 caaatgtcga tattgccatc gaaaaacagg tttgcactgc cggagaagtt ggtctttcgg
    23401 gtgaaatacg accgattcat cgcattgaac aacgaatttt ggaagccgaa aaactgggat
    23461 tcagaaaaat gattgttcct gctaataatt taaaaggtct ttcactcaaa aatatcgaaa
    23521 tggaaattat accggtgaaa aaagtggaag aggcttttaa aatattgtta aaaaaatgag
    23581 gtgtcgggtc gtttaagggg aaatgctctt ttttttctat gttttgaaaa ataatttttc
    23641 tttaccttat tatttcccct gaaaaagttt tattcattac taaaatttcc gaattataat
    23701 tttttcaagc tttcggaaga gtaacaataa aagttgttcc tttatctatt nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ataatttttt caagctttcg gaagagtaac
    23881 aataaaagtt gttcctttat ctatttgggt agtgaaagaa atatttccac ccgtaacttc
    23941 aataatattt ttagaaattg caagtcccag ccccattcct gtagatttgg tagtaaaatt
    24001 cggaatgaac aatttgtctc tgatatcttc aggtatcccg gctccattgt cgctaattcg
    24061 aatttcgatt tgctgatgag aagttgaaat tttcacctct atttttcctt gtctattttc
    24121 gggaatggat tgtatggcat tcttaatcag attattgaaa acttggatca attgttttgg
    24181 gtcagcaaaa gtgtaaatcg gttctttgga accttcaaaa gaaatatcga ctttttcata
    24241 attatgtttg aagaactcca cttccgactt cagaagctct cccaactcta ccctttcaaa
    24301 ttttgcttcc ggcatacggg caaaatcgga aaatgcacgg gcaatttgcg aaaggttatc
    24361 aatctgttcc accaacattt gagaagcctt atcaaaatac tcgttgaatt tttccttatc
    24421 catttttttt gttctgctca attgctgaat agtaagtttc atgggagtaa gcggattgtt
    24481 gatttcgtga gccacctgcc gtgccatcga tttccatgcc gattcccttt cggaacgtgc
    24541 caacaggcga gcactctctt ccaactcgtc cactgtccgg ttatattgag caaccaattg
    24601 gcctatttcg tcatcataac gataatcgat tttttcgttt cttcttccca agcgtatttc
    24661 tttgagtttt ttctccagca tcatcagtgg agcggaaatc tgattgccaa taagcaaggt
    24721 cagtataata accaataaaa tgataacaaa atatatgtga acaatcaccg aaataaaact
    24781 ttcaatttca ttctgaattt catcctgact gaagaactga ggaacggcta tgtatccgat
    24841 ttgcagataa tcgccattga aaaaatcggt ataagccgtc aaataattca aatggcctat
    24901 gttttcatac tgattgatat tggcatcttc agtaaaatat ggctttggcg caattcggtt
    24961 gctgatcaat tttttcgaaa aaatcaaagg ctgtgaactg cccaccaaaa ttccgtcatt
    25021 gttgtaaaca tgcacatcag tatggaaagt ataggaaaga tcctgcaaat ccaaatttaa
    25081 tgtctgggta tttttatcgg acaaatcttt gttccaataa tacaaatttt gaagcgcctt
    25141 ctgaatgtaa atttttttgt tctccagatt ttgaatctgt tctttctcgt aattgttacg
    25201 aatatagtca accgaagaat aaaaaattcc gataaaactc aaaattaaaa gagcaatgaa
    25261 tgaaatttgg aaccggttga taaaactcaa agtaaatttc cttccttttg cgaaaaaatc
    25321 atcaacttgc tggagtaacc aacacaaagc aaaaaaagcc agaaaagcat aacaaaaata
    25381 caacaaatat gcgccttctc catattgaac attctctgtc agaaccagat aagtattgcg
    25441 gtcgggggca taaacataat gacgtcgatc ggcataatga actacaaaat aattggaatt
    25501 ctcgaacgga atccaattag aaacatcggg gtaaacaaaa tttccatttc tatagaccaa
    25561 ctttcgatga tcgtaccggg caagcgaaac attcaactgc gacaaaatgg aaggcggaga
    25621 tgaaatcagt agattgggaa aactatagct tctgaaattc tgacggggat agaattccat
    25681 aaaataacaa gtagaatcat tgccggcagt taggacagaa aaaactccca aatatgaaat
    25741 attcttttcg ttcggtataa tgttgtagaa atgagtctgt ttgattttta ttcctttttc
    25801 ctgcaaaaat aactgatatt ccttgcacaa atccgagttt tttgaaactg cattaatatg
    25861 tacttcaaaa cggttccaaa aaccacgcaa gtaactattg ttcaaatact cgcttaaaac
    25921 ttccaccgaa tcggagtttc tcatcaactg atttacaaag cggtcgcgac ttaactgggc
    25981 atccaattct tccatcagaa tttctgtcat ttggtcgttt tcaatggtac cattcagtga
    26041 aagattctcg gccgcaattc tgtacttgtt aaatttcttt tcattggata aaaccaccac
    26101 atttccaata aaaaatatcg aaaagaacaa aacataaatt atgagtttga catgtctttt
    26161 tctggaaaaa tttacaatat aaaagccata caagggtatc cagagaaata agtaggagaa
    26221 aaaagaaagt ttccacattt cgggaaaaac cttgaaagct gccatcgcaa agagacaaga
    26281 aaaaatcgca tcgattgcaa aggcaatttt tatataataa tgtctggtca gaaaagtatg
    26341 aattttgaaa aacaacaagg caaatccaat tccccacaga agcattaaaa tatgcatcca
    26401 cacggtaaat atggaaaaat ttttaaagct gagaataagt atttgcggcc cggaatgata
    26461 aactactcct cgtaaaacat aataaaacaa caaaaaatac aaaggcaaca ttagcagcaa
    26521 tccgaaactt ctcaaaaaag aaacactatt tcccaactga acatgacgat aaaacaaaaa
    26581 aaccgtagaa aaaatgtaaa cagtaagcaa ggtcaaatgt accgttgaat ttaaaagtgg
    26641 cggaactgca tacaaataag gcgcaaaaag cttgttggca agtaaaaccg aaggaaaatt
    26701 aaaatacagc atcactgcca acaacgcacc ggtaatcaaa acataaccac aaaaatcttt
    26761 ccattgcaac gattttgtgc gcgaaagaga aaatcctctt gcataccaca caaaaaacag
    26821 caagaaaaac aaaagaaaag aaattaagcc agccaaagcc caacgttcat catacacggg
    26881 cactgtaggt tcggagagtg aaaacaaata atcgccgtgt gaactgaaaa ctgcatactt
    26941 atccaaattt gaacctccga caattccgac gtttttgttt aaattgaaac ctttggcaaa
    27001 atcattgatc agttgatcgt tttcgtaagg aaaattattc tttatcttaa tcaatgccaa
    27061 caatttcaac gaatcggcag aaagcgtttt gctcacacaa tatgcattgg gcaactgaac
    27121 aaaatgaagc tcaacgctgt cgttcaacga aatactgctg acgtccaaat gatgatccga
    27181 ccaaaaaatc agctcattgc cccgataaac ataaaaagat atatcatcgt cgggaaaaga
    27241 aaaattaatc agttcgttaa ccgaatgatt atcaagcaaa ttactcattt tcttcaatat
    27301 acgagcagta gataattctt tgcttatcag cttactttga aactggttga aatgtacgac
    27361 acgctgaggc gaagtttgat agaaatattc gcaaaaacat cctgccaaca caaaaacaag
    27421 cagaaatcca aaaaacgata ttttaccgct ttgcctcata aaaaattttt tctctcaaga
    27481 aatcatttgt ccttttttca tttcaagcat gatgataggg ttcacccttt aaaatagtca
    27541 tggctctgta gagttgctcc acaaaaataa gtcttaccat ttgatgcgaa aaagtcattc
    27601 gtgaaagcga aaccatattg tttgccctgc gatacaactc ttcagaaaag ccataagctc
    27661 ctccagtaac aaaaactatg cgcttattgg aagaatgcat tttttttgat aaaaaatctg
    27721 caaattctac ggaagtaaat tccgtgcccc tctcatccaa caaaatcaca tcatcgccga
    27781 tttcgagctt ttttagaatt aaatccgatt ctttggccgc ctgttcggaa gaagacaaat
    27841 tttttgtatt ttttaattcg ggaatattta tcacttcaaa attcaaataa tgtttcaatc
    27901 gattttggta ttgttcttgc agtgaagaaa ggtgaatatc aagagttttt ccaatattaa
    27961 tcagaacaaa tttcatcaga atataattta ttttttaaag gtctgatgga acccacatgt
    28021 ct
//
LOCUS       DAOTAX010000024        26578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1499_length_26578_cov_33.762357, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000024 DAOTAX010000000
VERSION     DAOTAX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26578
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_26578_cov_33.762357"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLM04_04145"
     CDS             <1..798
                     /locus_tag="PLM04_04145"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HON02303.1"
                     /translation="QSIANTPMKKVLIIILLFGVQISFFVVHAQKNGLIGKSVIPNSK
                     SIFFAAAGPAYCFGDVGGSHYEQFLNGINDWDVLNTRFLFSLGYGQTFKHNIGYKLIF
                     NYGNFTGNDKNSRNEARQYSFNSEVSSLSLQAEYTFLGGIHSNYYDYYSLYLFGGIGV
                     MNSNAEVKKNGVAISQPPPDRIYDKIKLNETAAFIPFGIGYKYSITPKFSIGAELSWN
                     YAFTDFIDGISTVTSKENDLLANLSITFSYKFFDSLTPACRCLPYNQ"
     gene            complement(804..1589)
                     /locus_tag="PLM04_04150"
     CDS             complement(804..1589)
                     /locus_tag="PLM04_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HON02304.1"
                     /translation="MLCKVKTLFVILFCAINFALSAYDVTGHRIIASIAYQNLNAATR
                     NRVDKLLGKQGIVYEAGWADEIRSDDNYAYSYQWHYQNLKDSLTSKDLKFLLEHPKVE
                     GKHLFFALDSLSGELKKNPNNAEALKFIIHFVGDLHQPMHLGRVDDLGGNKIMLKWFG
                     TKSNLHRVWDSQLLESSKYSYSEYSIYLQNKFEPQRATYQKFSLLQSAEAVYGLRNQI
                     YAYDLTTETNNYKYIYLFSDKLDEMLYLGGIQLANVLNGIYSK"
     gene            1779..2702
                     /gene="xerD"
                     /locus_tag="PLM04_04155"
     CDS             1779..2702
                     /gene="xerD"
                     /locus_tag="PLM04_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HON02305.1"
                     /translation="MNSLLTDYHYYLKFEKALSENTIQAYERDLEKLNSYLSDFHVLL
                     QDATIELLRDFIIEIGSLGIHPRSQARIISSISSFYRFLVYKNIISTDPSELLESPKI
                     GLRLPQILSVEEIDAMISAIDLSKPEGQRNKAIIEVLYGSGVRVSELINVKLSQLYLN
                     EGYMMVEGKGSKQRLVPLSPNSIKQIELWKIDRNHLEIKKGHEDFLFLNRRGEKLTRA
                     MIFKIVKDLATMAGIQKNISPHTFRHSFATHLLENGANLRAIQQLLGHESITTTELYT
                     HIDVQFLRETILKYHPLNQSKNEENKNLSDF"
     gene            2671..5094
                     /locus_tag="PLM04_04160"
     CDS             2671..5094
                     /locus_tag="PLM04_04160"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HON02306.1"
                     /translation="MRKIKICRISEKSLLLFRFMMGILLTEVLFVAGLQAQETIVVGQ
                     VIDKYDKHPLSSVSIYFKGTSVGTTSNEEGYFLLRHTGKENILVFSAIGYKKTEIKIK
                     PGSNIGLTVEMEESNTLLQEVFVLPGANPALDLMRKVRLLRKVNDETKLPHYEVLATE
                     QNAIFLSRSQHRSISKQIFRQLQEGNLLSSDSSLLVPLYMDEIKYQLTSTAKTELSKK
                     IFSSSEEGNKILEQLFGELNPSLNFYENSIPVFNKNIISPLASIGNMYYNYYLADSMV
                     TSSGKQYEIHFRSRNPRNPAFNGKFCLDSASLALTYIEAELPSEANFNFIRHLSIKQE
                     FVPFSSKRWVRNKDQLSLVLNLDILTDSVPAGSQIFFKRSTIYNTGSIQIPESFAQSG
                     YSEETLNQKLQQLGKTPLMNAAKWLADIIFTGYIPVGKIDIGKIQYLARTTDVEGLRL
                     TLPFRTNEKMWKNFTVGGFVGYGFKNEAIKYSGFTQYKLPAEKRRIIGLNYTNDYRRI
                     DYNYNNFLYLENPLVSGDEDIAGTIFALRSAGRLNERKEWTLSFSGDWNRNIESGLYF
                     RSLQQLPSDALPLKKDGVDIGNFQQQSFTFSTRFSFNEKKYEDHLQRIYINNRNPIIY
                     GILEVGKYELGNTEKYYGKVSGILKQNVQFDLGQVNYWMEAGSIFGSVPYPLLAYQPG
                     AEGGYGFYRFSMMNFMQFAADKYFDFHGELIMNGLILNHIPLLKYLNMREMFSVKFGY
                     GKMSNDHLSVMDFPAYVYPFNRPYTEVGVGLANILHLFNLQAIWRLTDIKQFSGDNFA
                     IRGSLRISF"
     gene            complement(5103..6137)
                     /locus_tag="PLM04_04165"
     CDS             complement(5103..6137)
                     /locus_tag="PLM04_04165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON02307.1"
                     /translation="MQVSVVILNWNGKNYLQQFLPILLEKTRLSYVEIVVADNGSTDG
                     SLQLLKEQFPMVRIILFDKNYGFAEGYNKALSQIKSDYYVLLNSDVEVTDNWLLPLVD
                     YMEKHPQTAACQPKILSYFNRTHFEYAGAAGGFIDYLGFAFCRGRIFGEVEEDKGQYD
                     DPMNVFWTSGACMMVRAGLYHKMGGFDADFFAHMEEIDLCWRFNSRGYQLTCVPESMV
                     YHIGGGTLQSEHPFKTYLNFRNNLLMLYKNLPKKQLKSVFFKRAILDYLAVFQFLASA
                     KPKNARSILKARSDFRKMKPNFLDKRKENILFSTATSFPMIYPRSIVVDYYLRKKKKF
                     TTLKFDQKPN"
     gene            complement(6291..7181)
                     /locus_tag="PLM04_04170"
     CDS             complement(6291..7181)
                     /locus_tag="PLM04_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HON02308.1"
                     /translation="MFYVIYPLLWLLSLLPLKVLYAFSPLMYFLIYRVAGYRKKVVRQ
                     NLQNSFPELSASERRKIEKNFYRFFADLLLEIVKLLYISDKELLQRVKFVNVEEFFEC
                     CRQGKYPILMLGHYGNWEWILTISLLMPENCESFTVYKRLKNRKFDQFMKSLREKRGA
                     VMVEKRNLARSMIRWAKEGKFGAYGFIADQSPSGKNLRNWTTFLHQPTAILPGAEELA
                     RKFNYPVFYARVSRPKRGYYSCEILPIAREPRTTSDNEISTRFMQLLEEDILAHPELW
                     LWSHRRWKIKPLIDEVKKDN"
     gene            complement(7284..7616)
                     /locus_tag="PLM04_04175"
     CDS             complement(7284..7616)
                     /locus_tag="PLM04_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02309.1"
                     /translation="MNTNEDNLLEYSDEEAVKFILKSLPAEVKDRIDEDTVQYVLDVI
                     YDFYNEKGYIDEDDTAEETSIDEEEMYEYILENALKDEINLTEDDIQMILDAEFDYGK
                     SLGIYREE"
     gene            complement(7737..8444)
                     /locus_tag="PLM04_04180"
     CDS             complement(7737..8444)
                     /locus_tag="PLM04_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HON02310.1"
                     /translation="MNNKHPYRYVFLDLDDTIWDFHANARLSLQKMYEQQTLNRYFEN
                     FDEFFRIYAMRNMELWEMYGKGEITKDFLMFERFRYPLAKMGLDDLQLAEAIGKQYLE
                     ILPEQTTLLPFARELLDYLHSKYPLTLISNGFVEVQYKKLKSCQIEHYFSHVVLSEQA
                     GALKPDPEIFNYALHLNQASPEEAIMIGDSFSADIVGAKNAGIDQIYLQKNNMLPYTK
                     QQATFIVKSLEEIFSIL"
     gene            complement(8568..9461)
                     /locus_tag="PLM04_04185"
     CDS             complement(8568..9461)
                     /locus_tag="PLM04_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02311.1"
                     /translation="MNKNISYRNIRVFSVIFLVAFLTVSCGKQSSDYKKLKAENDSLL
                     KEKERLEREVDDYFSSINQIQENLEKIKSTENVISVEPAGEELSQDARTRINEDINYL
                     NELLQTNKDELARLKSRLSKSSLKLTELERTITRLTKTLEEETARIAFLEKKLAEKDT
                     LIAQMGSTISQMGENINTLTTENKSKEEKIKQLDENLHSAWYVFGSRKELIDQKILTS
                     KGIFRKAEVLKDSFNKDYFVRIDARNAKTIPLYASKAKILTTHPTSSYTIEKQDGKLV
                     LIIVDPTAFWSVSKYLVIEVD"
     gene            complement(9592..10335)
                     /gene="rsmI"
                     /locus_tag="PLM04_04190"
     CDS             complement(9592..10335)
                     /gene="rsmI"
                     /locus_tag="PLM04_04190"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HON02312.1"
                     /translation="MSQQKNKTSLAGTTINNFSASKEGGKLYVVPTPIGNLEDMTFRA
                     VKILKKVNLILAEDTRTSSFLLKHFGIETPMQSHHKFNEHKTVENIVQQLKSGQILAL
                     ISDAGTPAISDPGFLLVRECVENGIEIECLPGATAFVPALVTSGLPNDRFCFEGFLPV
                     KKGRQQRLKELETEKKTMIFYESPFRLLKTLTQLSEVFGENRKASVSREISKMFEETV
                     RGTLAELIQHFTEKTPKGEFVLIVAGFEE"
     gene            complement(10463..11356)
                     /locus_tag="PLM04_04195"
     CDS             complement(10463..11356)
                     /locus_tag="PLM04_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02313.1"
                     /translation="MNKDLILALLHKNIQDLDMLTESFMEMNELPPAILHLAMQKVDE
                     IHQYLEELNENNSTKKPAGKEIFPSEDLKDKKENNFLHFSEHSTNDFAEPSENVSQQT
                     EIEAIKEMEFQVGEENIAQETEKTEFSFTESPINEKLTEWDETEKETAEIEQEPLENE
                     KNRFVSTQPEIGGETTEITDSEAQEKEEPSANEGQKTAPTSIPLQEKFSKIPDNSLGT
                     TFANQKIDDIKKAISIGDRFRFQRELFRGNGEDMNKTLNYLNQLATLEEAVSFLQAKY
                     KWDGENETVQDFFQILKRRFL"
     gene            11543..12133
                     /locus_tag="PLM04_04200"
     CDS             11543..12133
                     /locus_tag="PLM04_04200"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HON02314.1"
                     /translation="MFYSEQTSHASRRGSIEVICGSMFSGKTEELIRRLKRAQFAKQR
                     VEIFKPVIDTRYSESEVVSHDRNAIRSTPVGSSGSILLLSANVDVVGIDEAQFFDDGL
                     VEVCNQLANQGIRVIVAGLDMDYQGVPFGPIPALCAIAEDVFKVHAICVRCGALAYVS
                     HRLVDSNKRVLIGEMQEYEPICRNCFNEIQRSQMKI"
     gene            12154..13326
                     /locus_tag="PLM04_04205"
     CDS             12154..13326
                     /locus_tag="PLM04_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HON02315.1"
                     /translation="MMEQTERPYTFDRVVRLVITLTVLTALFFLTQRLSGVLLPFLIA
                     WLLAYLLQPIVHFFQYKLKFKYRFVAIFATLLLFIIILTGLGFLLIPMIINEIEKAVV
                     LINSFLRNVDVNSFLPIAWQNQLSEYFRQLDLQTLLGSEKFMSLVQKLLPEVWNFVGN
                     SLNFILGLAVIVIIFLYLFFILMDYEKISEKWIELVPMKYRPLITNIVQDIQDGMNRY
                     FRGQALIAMCVGILFMIGLSIIRLPLAIVIGLSIGILNLVPYLELLGIIPCAFMALLK
                     ASETNQNFWMIALSIAAVWLIVQSIQDLILVPRIMGKYTGLHPALILLSLSIWGSLLG
                     FAGMIIALPFTSLILSYYKRFIIKGENIEHPNVPQPEQTVSPVSSEECVSEEPKQD"
     gene            complement(13364..15088)
                     /gene="recJ"
                     /locus_tag="PLM04_04210"
     CDS             complement(13364..15088)
                     /gene="recJ"
                     /locus_tag="PLM04_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HON02316.1"
                     /translation="MTNKWIFKSLSEEEKIIQKNLVESLSINPTLAQLLVQRGIQTFE
                     EARSFFRPDLAELHDPFLMMDMDKAIERLNKAIDNKEKILIYGDYDVDGTTSVALVYQ
                     FLKKYYSDIDFYIPDRYTEGYGISFQGIQFAAEQNFSLIIALDCGIKATEKVKHALNL
                     GIDFIICDHHTPDKELPPAVAILDPKREDCPYPYKHLSGCGVGFKLLQAFTMKKGIDF
                     SELIPLLDLVGLSIASDIVPITGENRILAYYGLKRLNSLPSLGIRSIINVCGLADREI
                     TISDIVFKIGPRINASGRMKSAREAVELLIANDAEIARQKSGNINEYNNDRKDIDKNI
                     TDEAISIINSQDVYKKRKSIVVYQPHWHKGVIGIVASRLSDEYYKPTVVLTRSNGLIS
                     GSARSVPGFDLYKAIDSCRDLLENFGGHMYAAGLSMKEENIQAFTERFEQFVSENILE
                     EQTYPQLEIDAVLEFKDITPKFFRILKQFAPFGPENMKPLFVSKKIFDYGTSRLVGKE
                     QEHLRMELIDSSSENVMNGIAFGMHEYNDHLKALHPLDICYTLEENNFNGNSTIQLMI
                     RDIKTEEY"
     gene            complement(15283..16434)
                     /gene="obgE"
                     /locus_tag="PLM04_04215"
     CDS             complement(15283..16434)
                     /gene="obgE"
                     /locus_tag="PLM04_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HON02317.1"
                     /translation="MSGSNFVDYVKIFCRSGKGGAGSTHFHREKFIPKGGPDGGDGGR
                     GGHVILRGNKNYWTLLHLKYARHVFAEDGENGSKNRSFGKDGNDKIIEVPCGTVAYDG
                     ETGEFICDIQEDGQEFVLLQGGRGGLGNWHFRSATQQTPRFSQPGEPRKERTVILQLK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPQLGIVAYRDNKSFVMADIP
                     GIIEGAAEGKGLGLRFLRHIERNSILLFMIPAEEVDIREKYNILLNELKKYNPSLLEK
                     SKILAISKSDLLDAELKEEMRKNLPADVPTVFISPLTGEGILELKDLIWDELNKPSNQ
                     IIEISHAPIPISNQENEEELEEDFFPDNISSDMEETDRFEKMDWEDWDE"
     gene            complement(16470..17051)
                     /locus_tag="PLM04_04220"
     CDS             complement(16470..17051)
                     /locus_tag="PLM04_04220"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HON02318.1"
                     /translation="MLNIIICGAPGSGKGTQSKLIAEKYNLKHLSTGDMLRNEIKLQT
                     EIGKKAEQFISRGQLVPDEMIVAMLDHVLDKEMHHYKGIILDGFPRTFAQAEALEKLL
                     KKRNEEISLLLDLKVNDEELISRLLKRAEISNRSDDKLDVIRKRLEIYYQNADEVNNF
                     YKKTNKYVAIEGVGTVEEIFSRISQIIDSKINN"
     gene            complement(17072..17611)
                     /gene="hpt"
                     /locus_tag="PLM04_04225"
     CDS             complement(17072..17611)
                     /gene="hpt"
                     /locus_tag="PLM04_04225"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HON02319.1"
                     /translation="MEIVQIKDKKFKKFIPEQEILSSIRKIGEEINKTLKDKNPLFIS
                     VLNGAFMFTSDLMKVVNIPCEITFIKLSSYEGLYSSGSVKEVMGLNENIVGRNVVILE
                     DIIDTGLTMEQIIDSLKAKGAGEIRVATFLQKPDALQRNIKIDYIAMKIPNEFIIGYG
                     LDYNGYGRNLKDVYTLITD"
     gene            complement(18387..18995)
                     /locus_tag="PLM04_04230"
     CDS             complement(18387..18995)
                     /locus_tag="PLM04_04230"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HON02320.1"
                     /translation="MLIKIYETNPNPHEIHRVIETLRDGGTIIFPTDTVYAFGCSLFN
                     KHGIEYIARIKKKDLRSSQLSFVCESIKQISEYTKLDDETFKLIKKNLPGPFTFILKG
                     NHRLPKLFKDKKTVGIRMPKNNIALTIVHELGNPLMTSSIFYHNNSLEYIVDPEMIEE
                     KYGHLVDLVIDGGPGGYIPSTIVDCTCEEPVILRQGAGELIW"
     gene            19435..21930
                     /locus_tag="PLM04_04235"
     CDS             19435..21930
                     /locus_tag="PLM04_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HON02321.1"
                     /translation="MKIIHYLLLTCILASSCSRQVSLREVIDFTQDWKFTLSDSAMDA
                     SSPAFDDGAWRTLNLPHDWSIESDFSEKFPATAGGGALPGGIGWYRKTFFVPQSEQNK
                     CVYVDFDGVYRNSEVWINGHYLGKRPYGYISFRYELTPFLKFGEKNTLAVRVDNSKQP
                     NSRWYSGSGIYRNVWLTITQPIHVDLWGTYFTTPVVNENAATIQIQTSVRNSTGERRL
                     VKVEQVLLDNEGRKITKNETTIEVLPDSLAVASQSFSVNKPILWDIEHPYLYKMITKL
                     SVEGKETDEYETSVGFRYFNFDAQKGFSLNGKHLKINGVCNHHDLGALGAAINKRALE
                     RQLQLLKDMGCNAIRCSHNPPAPELLQLCDSMGFIVMDEIFDMWFKKKSAYDYAQDFP
                     KWYKTDMTDWIKRDRNHPSVFMWSIGNEVMEQWEDNITTDNMNLDQANLLLNNLKISD
                     SPKAKISESSLLTKHLSDIVKLLDPTRPVTAACNGTQKNNPLFLSGGLDIIGFNYHHN
                     EFMDVPKNFPGKPFIVTESVSAVQTRGFYLMPSDSIYIWPESWDKPFFNPLQKCSAYD
                     NSHVPWGSTHEKTWKIVKKHDFISGQFIWTGFDYLGEPTPYSWPSRSSYFGIIDLAGF
                     PKDIYYMYQSEWTDKDVLHVFPHWNWQKGQTIDVWAYYNNADEVELYLNGRSLGKRDK
                     KGDDLHVMWRVKYEPGTLKAISRKDGKEVLAYEVKTAGAPVKIRLTADRSTISSNSKD
                     LSFVTVELLDKDNNVHPLADQLVKFTLKGPGMIAGTDNGNENDSISLKKPQRHLFFGK
                     CMAIVQSTEKKGKITLKATVDGLPDEQIVITSK"
     gene            22145..22771
                     /locus_tag="PLM04_04240"
     CDS             22145..22771
                     /locus_tag="PLM04_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HON02322.1"
                     /translation="MSLEDYILQHSDSEPAYLTKISRDTHVKLLNPRMVSGHLQGRVL
                     SMLCKMIQPHKALEIGTFTGYSALCIAEALTDEDVLHTIEIDDELEDLILENFAGSEH
                     GHRIKLHIGNALEIIPTLNEVFDLVFIDADKREYTAYFEAVFPKLRNGGFILADNTLW
                     NGKVVTEAKPNDRQTMEILRFNDLIASDDRIEKVILPLRDGLTIIRKK"
     gene            22777..23196
                     /locus_tag="PLM04_04245"
     CDS             22777..23196
                     /locus_tag="PLM04_04245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HON02323.1"
                     /translation="MTYTYELEMKVRDYECDLQGIVNNAVYQNYFEHTRHEFLQANHL
                     SFSELHQQGIDAVVARIEIAFKKPLLPGEVFLSKLYLRKEGVKYSFYQALFKKAEGTL
                     CAKAKVDTVVVINGKLASAFEPFEKLLADNHAVKGEE"
     gene            23291..23605
                     /locus_tag="PLM04_04250"
     CDS             23291..23605
                     /locus_tag="PLM04_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HON02324.1"
                     /translation="MSDFLETEEKIVQMLRTVYDPEIPVNIYDLGLVYNIDVDEDGHV
                     EIDMTLTAPNCPVADFIIEDVRMKVESIEGIKDVDVNIVYEPAWDKSMMSEEAKLELG
                     LL"
     gene            23683..24429
                     /locus_tag="PLM04_04255"
     CDS             23683..24429
                     /locus_tag="PLM04_04255"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HON02325.1"
                     /translation="MIYFLSDAHLGSRLVENPREHEQKLVRWLEQVRRDAEAIYLLGD
                     IFDFWFEYKTVVPKGFVRFLGKLAELSDQGIEIHFFTGNHDIWTFGYLEEEIGMKVHR
                     EPLTVKLGNKLFYLAHGDGLYTNEPKFLFIRKIFHNSFAQRLFQLVPPRLGQKFGYWW
                     SKKSREKVLSLDNKYLGEENENLVIFAKNYILNHEVDFLVFGHRHIALDLQLQQQKRV
                     IILGDFVGIFSYGVFDGENFRLEYFEPQGE"
     gene            complement(24436..24858)
                     /locus_tag="PLM04_04260"
     CDS             complement(24436..24858)
                     /locus_tag="PLM04_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HON02326.1"
                     /translation="MTINELQDEIIEQFSQFDDWMDKYALLIDMGNSLEPLENEYKTP
                     QNLIEGCQSRVWLGAQMKDGKIYFRGDSDAVLVKGIVYLLIHVLSGHTPDEILNADLY
                     CIDKIGLREHLSPTRSNGLLSMLKQMKMYALAFKSKQE"
     gene            complement(24926..26149)
                     /locus_tag="PLM04_04265"
     CDS             complement(24926..26149)
                     /locus_tag="PLM04_04265"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HON02327.1"
                     /translation="MNKEEIEHIRADFPILSEKVYNKDLIYFDNAATTQKPRCVVEKI
                     EYAYFHLNANIHRGVHYLSLKATEAHEAARLTAAEFLHAEKKEEIIFTRGTTESINLV
                     AFSFGEAFCHPGDEVIVSAMEHHSNIVPWQMLCERKGMKLRVIPVNENGELDMVAYSS
                     LLNEKTRIVSVAHVSNVLGTINPVKEIIRIAHEKNIPVLIDGAQAVPHLSVDVRELDA
                     DFYVFSGHKIYGPTGIGILYGKEKWLNAIPPYQGGGEMISTVTFEKTTYNELPFKFEA
                     GTPDYVGSTALAEALRYINNIGLDNIAAYENELLHYAEQQLMEIENMHIIGTAKNKCS
                     VLSFLVDGIHPYDIGMLLDKLGIAVRTGHHCAQPIMDMFGIPGTVRASFAFYNTKEEI
                     VRFTEALKKVVNMLK"
BASE COUNT         7932 a         4996 c         5192 g         8458 t
ORIGIN      
        1 caatccattg ccaacacacc catgaaaaaa gttttgatca taatattgct ttttggtgta
       61 caaatttcat ttttcgtggt ccatgcacag aaaaatggat taataggtaa aagtgtaatt
      121 cccaactcaa aatcaatttt ttttgctgct gccgggcctg cttattgctt tggagacgtt
      181 ggtggttcgc attatgagca gtttttaaac ggtattaacg attgggatgt gttgaatacc
      241 cgatttttgt tttcgcttgg ctacggtcag acatttaaac ataatattgg ttataaactg
      301 atttttaatt acgggaactt taccggtaac gataaaaatt ccagaaacga agccagacaa
      361 tattccttta attctgaagt ttcaagcttg tcgttgcaag cggaatatac ttttttggga
      421 ggcatacatt caaattacta cgattattat tcgctttatc tttttggggg cataggtgtg
      481 atgaacagta atgctgaagt aaagaaaaat ggagtggcta tttctcaacc tccgccagac
      541 aggatatacg ataagataaa actcaatgaa acagcagctt ttattccatt tggaatagga
      601 tataaatatt caatcacacc taaattttct attggtgccg aactatcctg gaattatgct
      661 tttacagatt ttatagatgg tatttctact gttacttcca aagagaatga tcttttggct
      721 aatctcagca ttactttcag ttataaattc tttgacagtc tgacacctgc ttgtcgttgt
      781 cttccttaca atcaataatt tttttatttc gagtaaattc cgtttaaaac atttgccagc
      841 tgaatgcccc ctaaataaag catttcatcc agtttatccg agaaaaggta aatgtacttg
      901 tagttattcg tttctgttgt caaatcgtaa gcataaatct ggttgcgaag cccatataca
      961 gcttctgccg actgcaacaa actgaatttt tgatacgtgg ctctttgtgg ttcaaacttg
     1021 ttttgcagat aaatactgta ttcagaatag gaatacttgg aactttccag caactgacta
     1081 tcccacacgc gatgcagatt ggatttggtt ccaaaccact ttaacatgat tttgtttcct
     1141 cccaaatcgt caacacgtcc caaatgcatg ggttgatgca aatcgccgac aaaatgaatg
     1201 ataaatttta aagcttccgc attgttggga ttctttttaa gctcaccgga caacgaatcg
     1261 agcgcaaaaa acaaatgttt gccttcgact ttgggatgtt ccagcaaaaa ctttaaatct
     1321 ttcgatgtaa ggctgtcttt caagttctga taatgccact ggtaagaata ggcataatta
     1381 tcgtcgcttc ttatttcgtc agcccaaccc gcttcgtaaa caattccctg cttaccaagc
     1441 aatttgtcca cccgattgcg tgtagctgca ttgagattct gataggcaat gctggcaata
     1501 atgcgatgcc ctgttacatc ataagctgaa agagcaaaat taatggcaca aaataaaatg
     1561 acaaataaag tttttacttt acacagcata ttgattttta gtatattgat taaacaatcg
     1621 aaaagatgga acaagttgga ggacaaaatt agaaaaactt ttaagatgtt tgacaaaatg
     1681 gagaaaaatt aaatattttg cataaaaatg tagttttaac tttcctacaa aagttccttc
     1741 gagattttgt atctttgcaa cggttttaaa aattgtctat gaactcctta ctcaccgatt
     1801 atcattatta tctgaagttt gaaaaagcac tttccgaaaa taccattcag gcttacgaaa
     1861 gagatttgga aaaactcaac agctatctgt ccgattttca tgtattgttg caagatgcaa
     1921 ccatcgagct tttgcgtgat ttcataatcg aaattggtag tctgggaatt catccgcgtt
     1981 cacaagcccg catcatatcg agcatcagtt cgttttatcg ttttttggtt tacaaaaata
     2041 taatttctac tgacccgagc gaattgctcg aaagtcctaa aatcggactt cggttgccac
     2101 aaatattgtc ggtagaagaa attgatgcca tgatttcagc catcgacctt tcaaaacctg
     2161 aaggtcaacg caacaaagcc atcattgaag ttctctacgg ttcgggcgtg cgcgtttccg
     2221 aattgattaa cgtcaaactt tcgcagcttt atcttaacga agggtacatg atggtagagg
     2281 gaaaagggag taaacagcgg cttgttccgc tttcgcccaa ttcgataaag caaatcgaac
     2341 tttggaaaat cgaccgcaat catttggaaa tcaaaaaagg acatgaagat tttctttttc
     2401 tgaatcgaag aggcgaaaaa ctgaccagag ccatgatttt caaaattgtg aaggatttgg
     2461 caactatggc aggcattcaa aaaaacatca gtccgcatac ttttcgtcat tcttttgcta
     2521 cccacttgct ggaaaatgga gccaacctgc gcgccattca acagttgttg gggcacgaat
     2581 ccattactac aaccgagctt tatacccata tcgacgtgca gttccttaga gaaaccattt
     2641 taaaatatca tcccttgaac caatcgaaaa atgaggaaaa taaaaatctg tcggatttct
     2701 gaaaaaagtt tgttgctttt ccgttttatg atgggaattc tcttgacaga agttctattc
     2761 gttgccggtt tgcaggctca agaaacaatt gtggtaggtc aggttatcga caaatacgac
     2821 aagcacccgc tttcatcggt cagcatttac ttcaaaggaa catccgttgg aacaacaagc
     2881 aacgaagaag gttattttct tcttagacac acgggaaagg aaaatatact cgtattttcg
     2941 gctatcggat acaaaaagac ggaaatcaaa ataaaaccag gttcaaatat tggcctgact
     3001 gttgagatgg aagaatcgaa cacactgctt caggaagttt ttgttttgcc cggcgctaat
     3061 ccggctctcg atttaatgag aaaagtccga ctgttgagaa aagtgaatga tgagaccaaa
     3121 ctgcctcatt acgaagtttt ggcaaccgaa caaaatgcta tttttctctc tcgttcgcaa
     3181 caccgctcca tcagcaaaca gattttccgc cagcttcagg aaggaaatct gctctcttcc
     3241 gattcttctt tgcttgttcc gctttacatg gacgaaataa aatatcaatt gacttccact
     3301 gcaaaaacag agctttcaaa aaaaatcttt tcttcctctg aagaaggcaa caaaattttg
     3361 gagcagcttt ttggtgaact caatccatca ctgaattttt acgaaaattc catccctgtt
     3421 ttcaataaaa acattatcag cccactggca agcattggca atatgtatta caattattac
     3481 cttgccgaca gcatggttac atcttcggga aaacaatacg aaatacattt cagaagccga
     3541 aatccgagaa atccggcttt caatggaaaa ttctgtctcg attcggcttc gctggcactt
     3601 acctacattg aagcagaact cccttcggaa gccaacttca actttattcg acacctgagc
     3661 ataaagcagg aatttgtgcc cttttcgtca aaacgctggg tgcgtaacaa agatcagctt
     3721 tcactggtat tgaaccttga tattttaacc gacagtgttc cggccggttc tcagattttc
     3781 tttaaaagaa gtacaattta caataccgga agtatccaaa ttcccgaaag ttttgctcaa
     3841 agcgggtaca gcgaagaaac tctcaatcag aagcttcaac aattaggaaa aacacccctg
     3901 atgaatgcag ctaaatggtt ggccgacatt atttttacag gttacattcc tgtaggaaaa
     3961 attgatatag gaaaaattca atatcttgcc cgcactaccg atgtggaagg actgagactg
     4021 acattgccct tcagaaccaa cgaaaaaatg tggaaaaact ttactgttgg cggttttgtg
     4081 ggttacggat tcaaaaacga agccataaaa tattcaggtt tcacacaata taaattgcca
     4141 gcggaaaaaa gacgcattat cggtttgaat tacacgaacg attaccgtcg tatagattat
     4201 aattacaata attttctgta tctcgaaaat ccgcttgtaa gtggcgatga agatattgcc
     4261 ggcaccattt ttgcccttcg cagtgccggt cgattgaacg aaagaaaaga atggacgctt
     4321 tctttctccg gcgactggaa caggaatata gaatccggat tgtatttcag gtctttgcag
     4381 cagcttccat ccgatgcttt accgttaaaa aaggacggag tcgatattgg caatttccaa
     4441 caacaatctt ttaccttttc aacacgtttt tcgttcaacg aaaaaaaata cgaagaccat
     4501 ttacagcgaa tttatatcaa taaccgcaac ccaataattt acggcatttt ggaagtgggg
     4561 aaatatgaac ttggaaatac tgaaaaatac tacggaaaag tttcgggtat tcttaaacaa
     4621 aatgtccagt tcgatttggg acaagtcaat tattggatgg aagccggaag tatttttgga
     4681 agcgttcctt atccactgtt ggcatatcaa cccggagccg aaggcggata cggattttac
     4741 cgtttcagca tgatgaattt tatgcaattt gcagccgaca aatattttga ttttcacgga
     4801 gaactgatta tgaacgggct gatattaaat catattccgc tcctcaagta cctgaacatg
     4861 cgcgaaatgt tttccgttaa gttcggttac ggaaaaatga gtaacgacca cttgtcggtc
     4921 atggattttc cggcttacgt ttatcctttt aatcgtcctt acaccgaagt tggcgtcgga
     4981 ctggccaata ttctgcatct attcaattta caggcaatct ggcgattgac cgatattaag
     5041 caattttccg gcgacaattt tgctattcgt ggaagcttaa gaatcagttt ttaaaatatt
     5101 ttttagtttg gtttttgatc gaattttagt gttgtgaatt ttttcttctt tctcaaataa
     5161 taatccacca caatacttcg gggataaatc atgggaaagg atgttgcggt ggagaagagg
     5221 atattttcct ttcttttatc cagaaaattt ggtttcattt ttctgaaatc ggagcgagct
     5281 ttcagtatgc ttcgtgcatt tttgggtttg gcagaggcaa gaaattgaaa aacagccagg
     5341 taatcgagaa tggctcgctt gaaaaacacg ctttttaatt gctttttcgg cagattttta
     5401 taaagcatca acaaattatt ccgaaagttg aggtaagttt tgaatggatg ttccgattgc
     5461 aaagtgcctc ctccgatatg gtaaaccatg ctttcaggaa cacaggtcaa ttgatagcca
     5521 cgactgttga atcgccagca aagatctatc tcttccatgt gggcaaaaaa atctgcatca
     5581 aatccgccca ttttatgata aagccctgcg cgaaccatca tacaggctcc ggaagtccag
     5641 aaaacattca tcgggtcatc atattgtccc ttgtcttctt ccacttctcc aaaaatgcgt
     5701 cccctacaaa aggcaaatcc cagataatcg ataaaaccgc cggcagcacc tgcatattca
     5761 aaatgtgttc gattaaaata agaaagtatt tttggttggc aagctgcagt ttgtggatgt
     5821 ttttccatat aatccaccag cgggagcaac caattgtctg taacttccac atcggaattc
     5881 aacagcacgt aataatccga tttgatttgg gacaaggctt tattgtagcc ttcggcaaaa
     5941 ccataatttt tatcgaataa aatgatgcgc accattggaa actgttcttt caacaactga
     6001 agtgaaccat ctgtagaacc attgtcggcc accactattt ccacatacga caaacgcgtc
     6061 ttttccaaca gtatgggaag aaattgctgc aagtaatttt tcccgttcca attcaatatc
     6121 actaccgaaa cttgcatagt caactataaa agaattattt gttttttgcg gttttaattt
     6181 taaaaaattt tttttaaaat gtcgattaac attcagacgt tcagaaaaaa aatcaaaatc
     6241 tgtcgttttg ctgcaacagt tgatgttttg cgtcaaagcg aaagcgattg tcaattatct
     6301 tttttcacct catcaatcag aggtttaatt ttccatcggc gatgcgacca aagccaaagt
     6361 tcaggatgag ccaagatgtc ttcttcgagc agttgcatga aacgtgtaga aatttcattg
     6421 tcggaagtgg ttcgtggctc tcgggctatg ggcaaaatct cacaggaata atatccacgt
     6481 tttggtcggc tcacgcgtgc ataaaaaacc ggataattga attttcttgc caattcttcg
     6541 gcaccgggca aaattgcagt cggctggtgc aaaaaagttg tccaatttcg aagatttttt
     6601 ccactcggac tttggtcggc aataaatccg taagcgccaa attttccttc ttttgcccaa
     6661 cgtatcatac tccttgccag attgcgtttc tccaccatta cagctccccg tttttcacga
     6721 agcgacttca tgaattgatc gaactttctg ttttttaacc gcttgtacac ggtaaaagac
     6781 tcacaatttt cgggcattaa caagctaatg gtcaatatcc attcccaatt gccataatga
     6841 ccgagcatca aaattggata ttttccctga cgacaacatt caaaaaactc ctcaacattg
     6901 acaaatttca ccctttgcag caactctttg tcgcttatgt aaagaagttt aacgatttcg
     6961 agcaacaaat cggcaaagaa acggtaaaaa ttcttttcta tttttcttct ttcggatgcc
     7021 gaaagttcag gaaatgaatt ttgaagattt tgtcgaacca ctttttttcg gtaacctgct
     7081 acacgataaa tcaggaaata cattaatgga gaaaaggcat aaagcacttt cagcggaagc
     7141 agggacaaca accacaaaag cggataaatc acataaaaca tatgtttttt gttattttac
     7201 aaaaatacca aagataaaaa aacaccaagc aaaacctgca tgcgttggtg ttgagggaaa
     7261 aagtaggtat gtgaaaaacg aaattattct tctctgtaaa tacccaatga tttgccgtaa
     7321 tcaaattcag catccaaaat catctgaata tcgtcttccg taaggttgat ttcatctttc
     7381 aaggcgtttt ccaaaatgta ttcatacatt tcttcttcgt caatgctggt ttcttcggca
     7441 gtatcatctt cgtcgatata acctttttcg ttgtaaaaat cgtaaatcac atccaacacg
     7501 tactgaacag tatcttcgtc gatgcggtct ttaacttcgg ccggaagaga ctttagaatg
     7561 aattttacgg cttcttcatc gctgtattcg agtaaattgt cttcgtttgt attcatgatg
     7621 caatggtttt aactattaaa aacgttcaaa gatagtgctt tttttggttt tcgtcaaaaa
     7681 ataaattttt tgagcttttt ttgaattgta gataaacatg cagggaattt tgtctgctac
     7741 aaaattgaaa aaatttcttc gagcgatttt acgataaaag tggcctgttg tttggtataa
     7801 ggcagcatat tgtttttttg aagataaatc tggtcgattc ctgcattttt ggcgcccaca
     7861 atatcggccg aaaaactgtc gccaatcata atggcttctt cgggtgaggc ctgattcagg
     7921 tgaagagcgt agttgaatat ttctgggtcg ggtttgagtg cgccggcttg ttccgaaagt
     7981 accacgtgag agaaataatg ttcgatttgg caggatttca gttttttgta ttgcacttct
     8041 acaaatccgt ttgaaatgag tgtcagcgga tacttggaat ggagataatc cagcagctca
     8101 cgggcgaagg gaagcagagt cgtctgttcg gggagaattt ccagatattg ttttccaatg
     8161 gcttcggcca gttgcaagtc gtcaagtccc attttggcaa gcggatagcg aaaacgttcg
     8221 aacatcaaaa aatccttggt gatttctcct tttccgtaca tttcccaaag ttccatgttt
     8281 cgcatggcat aaattcggaa aaattcgtcg aaattttcaa aataacggtt gagcgtctgc
     8341 tgttcgtaca ttttttgcaa cgacagtcgg gcgttggcat gaaaatccca gattgtgtcg
     8401 tccaaatcga gaaaaacgta tcggtaagga tgcttgttat tcattcggta gcatttttca
     8461 gaaataaatt tctaaaaatc atacttgatt attccgccag cctaaaaaag gatttgaagt
     8521 ttcccgacgc ctgttttttt aaaattcttt cagacaaaaa aatgttgtta atcaacttcg
     8581 ataacaaggt acttgctcac actccagaaa gcagtcggat cgacaatgat aagcaccaac
     8641 ttgccatctt gtttttcaat tgtataagat gaagtcggat gtgtagtgag tattttggct
     8701 ttggaagcat acagcggaat ggttttggca ttgcgggcat caatcctgac aaaataatcc
     8761 ttgttgaagg agtcttttaa tacttcggct tttctgaaaa ttcctttaga tgtaagtatt
     8821 ttctgatcaa tgagttcttt tcgtgaccca aaaacatacc atgccgaatg aagattttca
     8881 tcgagttgtt taattttttc ttccttagat ttgttttcgg tagtcagtgt attgatattt
     8941 tctcccattt ggctgatggt gctgcccatt tgagctataa gggtgtcctt ttcggccagc
     9001 tttttttcca gaaaagcaat gcgcgccgtt tcttcttcca atgtttttgt gaggcgggta
     9061 atggttcttt ccagttcggt gagttttaat gaacttttgc tcaatctcga cttcagccgt
     9121 gccagctcat ccttgttggt ttgcagcaac tcgttaaggt aattgatgtc ttcgttgatt
     9181 cgagtgcgtg catcctgtga taattcttct cctgccggtt caacagagat aacattttcg
     9241 gtacttttaa ttttttccag attttcctga atctgattga tactcgaaaa atagtcatct
     9301 acttcgcgtt cgagtctttc cttttctttc agcaaggagt cgttttctgc cttgagcttt
     9361 ttgtaatcgg acgattgttt tccacacgaa acagtcagaa aagctaccag aaaaattacg
     9421 ctaaaaactc tgatgttgcg gtatgaaata tttttgttca tggggatgga aaattataat
     9481 ggtttggagt atggagaaaa tacgaattgt tgaatgaaaa aaattaataa tatgataatc
     9541 aataaattaa aataaaaatt ttacacttta aaatactgtg caaagataca gctattcttc
     9601 aaaaccggca actatcaaaa cgaattctcc ttttggcgtt ttttcagtaa aatgctgtat
     9661 caactcggct aacgtacctc tgactgtctc ttcaaacatt ttggaaattt cacgtgaaac
     9721 ggaagcttta cggttttcgc caaatacttc tgatagttgt gtcaaagtct tcagcaagcg
     9781 gaaaggcgat tcatagaaaa tcatcgtttt tttttctgtt tccaattctt ttaaacgttg
     9841 ctgacgtcct ttttttacgg gcaaaaaacc ttcaaaacaa aagcgatcgt taggcaagcc
     9901 tgaagttacc aaagccggaa caaaagctgt tgctccaggt aaacactcaa tttctatgcc
     9961 attttctaca cattcgcgta ccagcaaaaa acccgggtcg gaaattgccg gagtgccggc
    10021 atcggatatc agggctaaaa tttggccgct tttcagctgt tgtacaatat tttcaaccgt
    10081 tttgtgttcg ttgaatttgt gatgcgactg catgggggtt tctatgccga agtgtttcag
    10141 cagaaaactg ctggtgcgtg tgtcttcagc caaaataaga ttcactttct tgagaatttt
    10201 tacggcgcga aatgtcatat cttccaaatt gcctatggga gtaggaacca cataaagttt
    10261 acctccttct ttggatgcgg aaaaattatt gattgtcgtc cccgccaatg aagttttgtt
    10321 cttttgctga ctcatgattt tttaaaaaaa attcttttca gatgagaccc acatgtattg
    10381 catacacaaa cacgaatgag tataatattc agacatgtgg gttccataag acctttaaaa
    10441 aataaattat attctgatga aattataaaa atcttcgttt gagaatttga aaaaaatcct
    10501 gcaccgtttc gttttctcca tcccatttgt atttggcttg caaaaaagaa acagcttctt
    10561 ccagagttgc cagctgattg aggtaattca gtgttttgtt catatcttcg ccattccccc
    10621 tgaaaagttc acgctgaaat cgaaaacggt cgccaatgct gatggctttt tttatgtcgt
    10681 cgattttttg attggcaaat gtagtcccca aagagttatc gggaattttg gaaaattttt
    10741 cctgcaacgg gattgaagtc ggtgcagttt tttggccttc attggccgat ggttcttcct
    10801 tttcctgggc ttccgaatct gttatttcgg tagtttcacc tcctatttcg ggctgggtag
    10861 aaacaaatcg gtttttttcg ttttcgagcg gttcttgctc aatttctgct gtttcttttt
    10921 ctgtttcatc ccattcggtt agtttttcat taattggact ttcagtgaaa gaaaattccg
    10981 ttttttctgt ttcttgggca atattttctt cgccgacttg aaattccatt tcttttattg
    11041 cttcaatttc ggtttgctgc gaaacatttt ctgagggctc tgcgaaatca tttgtcgaat
    11101 gttcagaaaa atgtagaaag ttgttttctt ttttgtcttt caaatcctcc gaaggaaaaa
    11161 tttctttacc ggcaggtttt ttcgttgagt tgttttcatt cagttcttcg agatattggt
    11221 gaatttcatc aactttttgc atggcaagat gaagaatggc cggtggaagt tcgttcattt
    11281 ccatgaaact ttcggtgagc atgtccaaat cctgaatgtt tttgtggagc aaagccaata
    11341 ttaaatcttt gttcatattt ttttctttaa aagaatttca gtacttgtta ttttttttaa
    11401 ttttgtccaa gatattattt ttccaacctg aaatttttca gactataaaa aaattgtctt
    11461 taaaacgttt tagacggtaa agttacaata attgaaatag aaaatataca aagaaagcgt
    11521 aaatttcaac aaaataaatg atatgtttta ttcagaacag acttctcatg catcgagacg
    11581 tggtagtata gaagtgattt gtggttcgat gttctcgggg aaaacagaag agttgattcg
    11641 gcgactaaag agggcgcagt ttgccaagca aagagtagaa atatttaaac cggttatcga
    11701 tacgcgttat tcggaaagcg aagttgtttc tcacgatcgc aatgccatac gttcaacgcc
    11761 ggtaggttcg tcgggaagta ttctgttgtt gtctgcaaat gtggacgtag tgggtattga
    11821 tgaagcacag ttttttgatg atgggctggt ggaagtgtgt aatcaattgg ccaatcaggg
    11881 cattcgtgtc attgtggcgg gtctcgatat ggattatcaa ggtgtacctt ttggtcctat
    11941 tccggcattg tgcgctattg cggaagatgt gttcaaggtt cacgccattt gcgtgagatg
    12001 tggtgcattg gcttatgttt cgcatcgact ggtggacagc aacaagcggg ttttgattgg
    12061 cgaaatgcag gaatacgaac caatttgtcg taactgtttt aatgaaattc aacgcagtca
    12121 aatgaaaatt taacttttct ataattctgc aatatgatgg aacaaactga aagaccttat
    12181 acatttgatc gggtggtgcg tcttgtcatc acgctgaccg ttcttaccgc tctttttttc
    12241 ttaacccagc gattaagcgg tgttttgctt ccttttttga ttgcatggtt actcgcctat
    12301 ctgcttcagc ctatagtcca tttttttcag tacaaactta aattcaagta tcgttttgta
    12361 gctatttttg caaccttgct gcttttcata attattctca cgggtctggg ttttctgttg
    12421 attccgatga tcatcaatga aatagaaaaa gcggtggtgc ttatcaattc ttttttaaga
    12481 aatgtagatg taaattcttt tttgcccatt gcctggcaaa atcagttgtc tgaatatttc
    12541 aggcaacttg atttacaaac acttttaggc agcgaaaaat ttatgtccct tgttcaaaaa
    12601 ttattgccag aagtatggaa ttttgtcgga aattctttga attttattct gggacttgct
    12661 gtgatagtaa ttatttttct ttatctattc tttattttaa tggattacga gaaaatttcc
    12721 gaaaaatgga ttgaacttgt tcccatgaaa taccgcccct tgattaccaa tattgttcag
    12781 gatattcagg acgggatgaa tcgttatttc agagggcagg ctttaatagc catgtgtgtg
    12841 ggaattcttt ttatgattgg gctgagtatc attcgtttac ctttggcaat tgtgattggt
    12901 ttgagcatcg gtattctcaa tcttgttccc tatctagagt tgctgggtat tattccatgt
    12961 gcttttatgg ctttgctgaa agcttcggaa accaatcaga atttttggat gattgctctc
    13021 agcattgcag cagtatggtt gatagtgcaa tcgattcagg atttaatttt agttccgaga
    13081 atcatgggaa aatatacggg acttcatccg gctttgattt tgctttcctt gtccatttgg
    13141 ggttcgctgt taggatttgc agggatgatc attgcactgc ccttcacgtc tttgatattg
    13201 tcttattata aacgcttcat tataaagggc gaaaatattg aacatcccaa tgttccgcag
    13261 cccgagcaga cggtctcgcc tgtttcttcg gaagaatgtg tttcagaaga gcccaaacaa
    13321 gattaaactt attaaagcac agtcttttcg agcgaagttt caattaatat tcttcggtct
    13381 ttatatctct gatcatcaat tgaatggtag aatttccatt aaaattgttt tcttccagtg
    13441 tataacaaat atcaagcgga tggagggcct tcaagtgatc attgtattcg tgcataccaa
    13501 aagctattcc attcatcacg ttttccgagc ttgagtcgat cagttccatt ctcagatgtt
    13561 cctgctcttt tcccaccagt cggcttgtcc cgtaatcaaa aatttttttg gaaacgaaaa
    13621 gaggtttcat attttccggc ccaaaaggtg caaattgttt caggattcgg aaaaacttgg
    13681 gagtaatatc cttaaattca agaacagcat cgatttccaa ttgaggataa gtttgttctt
    13741 ccagaatatt ttctgaaaca aattgttcga agcgttcggt aaaagcctga atattttctt
    13801 ccttcatgga aagaccggct gcatacatgt gtccgccgaa attttcgagc aaatctctgc
    13861 acgaatcaat ggctttgtac aaatcgaaac ccggcaccga gcgagcagaa ccggaaatca
    13921 agccatttga acgcgtgagg acaacggtag gtttgtagta ttcgtcggat agacgagacg
    13981 caacaatacc aattactcct ttgtgccaat gaggttgata tacgacaatg gatttgcgtt
    14041 ttttgtaaac atcctgcgag ttgatgatgg aaatagcttc gtcggtaata tttttgtcta
    14101 tatccttgcg gtcgttgttg tattcattga tatttccact tttttgtcgt gcaatttctg
    14161 catcgttggc aataagtaat tccacagctt cacgagctga tttcatacgt ccgctggcat
    14221 tgatgcgtgg cccaatttta aacacaatat cgctaatggt aatttccctg tcggcaagtc
    14281 cacatacatt gatgatactc cggatcccca atgaaggtag ggaattaagt ctttttaaac
    14341 cataataggc aaggatgcgg ttttctccgg ttatgggaac aatatcggaa gctatgctca
    14401 gtcctaccaa atcgagcaaa ggtatgagtt ctgaaaaatc gattcccttt ttcatggtga
    14461 aagcctgcag gagtttaaat cccactccgc aacccgaaag atgtttataa ggataagggc
    14521 agtcttcacg cttgggatcg agaatagcta cagcaggagg caattctttg tcgggagtat
    14581 gatgatcgca aataatgaaa tcgattccga gattgagcgc atgtttaact ttttctgtgg
    14641 cttttattcc gcagtccaaa gcaataatca acgaaaaatt ctgttcagcg gcaaattgaa
    14701 ttccctgaaa cgaaatgccg tatccttcgg tatagcggtc ggggatataa aaatctatat
    14761 cggaataata ttttttcaaa aattgataga ccaatgccac agaagtcgtt ccatccacat
    14821 cataatcccc gtaaatcaga attttttcct tgttgtcgat ggctttgttc aatctttcaa
    14881 tggctttgtc catatccatc ataaggaaag gatcatgaag ctcagccaaa tcgggacgga
    14941 aaaaactgcg tgcttcttca aaagtctgga taccgcgttg aacaagtaat tgggcaaggg
    15001 tagggttgat gctcaaactt tctaccagat ttttttgaat tatcttttct tcttcggaga
    15061 gagacttaaa tatccattta ttggtcatgt cagtataatt tcaaaattaa agaattcgac
    15121 aacggaactt aaaagaagat actgtaactt tcgtttctca ggcattacaa aaatagcaaa
    15181 aaaagctgag gcgatgacaa aattttttct gatgcctttt ttgaaggcaa gaaaaatctt
    15241 taaatattgg tgggaaactt agaaaatttc aaaaaacgaa gattattcgt cccaatcctc
    15301 ccaatccatt ttttcaaaac ggtcggtttc ttccatatca gacgaaatgt tgtcggggaa
    15361 aaagtcttct tccaattctt cttcattttc ctgattcgag atgggaatgg gagcgtgaga
    15421 aatttcaatt atttggttgg aaggtttatt caattcatcc caaatcaaat ctttaagttc
    15481 aagaatacct tcgccggtaa gtggagatat aaaaacggtc ggcacatcgg ccggcaaatt
    15541 ttttctcatt tcctctttca gttctgcatc cagcaaatca cttttgctga tggcgagaat
    15601 tttcgacttt tccagtaagg aggggttata ttttttcagc tcgttcagca aaatattgta
    15661 tttttcccga atatcaacct cttcggcagg aatcatgaac agtaaaatgg aattacgttc
    15721 aatgtggcgt aaaaagcgaa gtccgagtcc ttttccttcg gctgcgcctt caataattcc
    15781 cggaatatcg gccataacaa aagatttatt gtcgcggtaa gccacaattc ccagttgagg
    15841 gacaagtgtg gtgaaaggat aatcggcaat ttctggtttt gctgcagaaa cgactgaaag
    15901 cagcgtggat tttcccgcat tgggaaatcc aaccaaacca acgtcggcta atattttcaa
    15961 ctgaagaata actgttcttt cctttcgcgg ttctccgggt tgagaaaatc tgggcgtctg
    16021 ctgagtagcg cttctgaagt gccaatttcc caaacctcct cttccgcctt gcaacaaaac
    16081 aaactcttga ccgtcttcct gaatgtcgca gataaactcg cccgtttcgc catcgtacgc
    16141 aaccgttccg cagggcactt caataatctt gtcgtttcca tccttgccaa agctacggtt
    16201 tttacttcca ttttctccat cttcggcaaa tacatgtcgg gcatatttca gatgaagcaa
    16261 cgtccagtaa tttttgtttc cacgcaaaat aacgtgcccg ccacgaccac catcgccgcc
    16321 atcagggcca cctttgggaa taaacttttc ccgatgaaaa tgagtggacc ctgcaccgcc
    16381 tttgcctgaa cggcaaaaaa tctttacgta atcgacaaag tttgaacctg acattttggg
    16441 aaaaggaatt taatgaaaga caaaggttat cagttattta tttttgaatc gataatttgc
    16501 gagatacgag aaaaaatttc ttccactgtg ccaacgcctt ctatggctac atatttattc
    16561 gtttttttgt aaaaattgtt aacttcgtcg gcattttgat agtagatttc gagtcttttc
    16621 cgaatcacat ccagtttgtc atcacttctg ttcgatattt cggcgcgttt aagcaatcga
    16681 ctaattaatt cttcatcgtt aactttcaag tcgaggagca gactaatctc ttcatttctt
    16741 tttttcagca gtttttccaa agcttcagcc tgagcgaaag ttcggggaaa accatccaaa
    16801 ataatgccct tgtaatggtg catttccttg tcgagaacat gatcgagcat ggcaacaatc
    16861 atttcgtcgg gaaccaattg ccctctggag ataaactgtt cggctttttt gccaatttct
    16921 gtctgaagtt taatttcatt tcggagcata tctccggtgg ataaatgttt taaattgtac
    16981 ttttctgcta tcagtttgct ctgtgttccc tttccacttc ccggtgctcc gcaaataatg
    17041 atattcaaca tttgttaatc tgttttctgt attaatccgt tatcaaagta tagacatctt
    17101 ttaaatttct tccatatccg ttgtagtcga gcccgtagcc aataataaat tcattgggaa
    17161 ttttcatggc aatgtaatct attttgatgt tgcgctgcaa ggcatcgggt ttttgtaaaa
    17221 atgttgccac ccgaatttct ccggcacctt ttgctttgag cgaatcaatg atttgttcca
    17281 ttgtgagtcc cgtgtcgata atatcttcca aaatcactac attacggcct acgatatttt
    17341 cgttcaatcc catcacttct ttcacactgc ctgaactgta aagcccttcg taagaggaaa
    17401 gtttgatgaa ggtgatttca cacggaatgt tcaccacctt cattaagtcg gacgtaaaca
    17461 tgaaagctcc gttgagaaca gaaataaaga gtggattttt atctttcagg gttttattga
    17521 tttcctcacc aatttttctg atggaagaaa gaatttcctg ttcaggaata aattttttga
    17581 actttttatc tttgatttga acgatctcca tttcccaaca gttgcttttg gatgatttaa
    17641 cgatgagttt taaaaaaata atttgcaaaa gtagtaaaaa tgattggttt ttgaaagtta
    17701 agttttaatt gcttgttttt cccgtatttt aattatcatg tcattgaggt ttcgtttaac
    17761 atcgactgaa atttatcgac attagcaaaa gttgtcttgc aatatcttcc gtcattaaaa
    17821 aaatttaaag tttagtccat cctgaaattt ctttgtctta aaacaaaaaa gaagttttat
    17881 cagagtagtt tcctttttat cgctgcatcc cgcaacagga aaaaaggtgt aaatcttgta
    17941 taaaaacaga gaaaacttta tctaagatat agaactttta caaaaaactt ctgcagttga
    18001 tgcatatctt gaaaaaatca acagtaaact ggttctcaaa attgttttaa ataagcagag
    18061 cttggttttc ctgattatgg aacagctctt ctgactttgg ctttcccttt aaattggtta
    18121 atgtgtaatg ggggttaaaa ttagcgtctt acaagttttt gtttgtgcca gaaaattttc
    18181 tttgtttctt taaattaata agctttaggg gaggtaactt aaaaaatact ttccgaagta
    18241 aaatgctgat cgtacaaatc atgatcatca atgcttatgc aaaaatgctt tcgataagtt
    18301 tcgaagagtt aaaaataatt gtataatacc ctcttgttcg ttttctttaa gtttttgtgt
    18361 tattgatttg caacataaaa aaatacttac caaatcagtt ctccagcgcc ttgcctcaaa
    18421 ataaccggtt cttcgcaggt gcaatcaaca atggtagatg gaatataacc tccgggtcct
    18481 ccatcaatta ccaaatcgac caaatggcca tatttttctt caatcatttc agggtcaaca
    18541 atatattcca gtgaattgtt gtggtaaaaa atagaactgg tcattaatgg atttcctaat
    18601 tcatgaacaa tggtgagtgc aatattattt tttggcattc gtattccaac cgttttttta
    18661 tctttaaaaa gtttaggcag gcgatgattc cctttgagaa tgaaagtaaa cgggccgggc
    18721 agattttttt ttatcagttt aaaggtttcg tcatcaagtt tggtatattc gcttatttgt
    18781 tttatggatt cacaaacaaa tgaaagttgg gagctgcgaa gatctttttt cttgatgcgt
    18841 gcaatatatt ctatgccgtg tttgttgaac aaactgcatc cgaatgcata aactgtatcg
    18901 gtaggaaata taatggttcc tccgtcgcgt aaagtttcaa ttacacgatg tatttcgtgg
    18961 ggattgggat tggtctcata aattttaatt agcatattct gtcgaaaagg atttgatcga
    19021 aaaaatttgt caaagatata aaaaatccgt aatataaaat attttttcac aaccaatgat
    19081 tcaacaaata attgattaat aagttaataa ttagtaaatt aagaattata ttgtaacaaa
    19141 atattactcc tcaccgataa acatttttaa gaaaaatgta aaatgaatgg gacaaatgct
    19201 taagcattca ccctattgtt gtatatttga attgcaaaat ttcaaacatt ggaacatggg
    19261 caatgataat gattacgaaa aaaatttggc cgggcatccg aattttaatt tatcacgttt
    19321 tatagcagtt aaaattaatg ttctaattat gagcagttaa attaaaaaaa aatacaccag
    19381 tttatcagaa aaaatcagaa tttattattg ttaaccttaa aaaaatctat agttatgaaa
    19441 attattcatt atttattgtt gacctgtatt cttgcaagtt catgcagccg acaagtatca
    19501 ctccgtgaag tcatcgactt tacccaagac tggaaattca ccttaagcga ttccgcaatg
    19561 gatgcttcaa gcccggcatt cgatgatggt gcatggagaa ctcttaattt accccacgat
    19621 tggagtattg aatccgattt ttctgaaaag ttccccgcca cagccggtgg aggagctctt
    19681 cccggtggca ttggctggta tagaaaaact ttctttgtac ctcagtccga gcaaaataaa
    19741 tgcgtatatg tcgatttcga cggtgtttat cgcaatagtg aagtttggat caatggtcat
    19801 tatctcggca aacgacctta tggatacatc tcgttccgct atgaattaac acctttctta
    19861 aaatttggag aaaaaaatac cttagccgta cgcgttgaca attccaagca gcccaattcg
    19921 cgctggtatt caggatcagg catatatcgt aatgtttggc tgaccataac acaacccatt
    19981 catgtggatt tgtggggaac ttattttaca actcccgttg tgaacgaaaa tgcagcgact
    20041 attcaaattc aaacttcagt aagaaattca acgggcgaac gacggcttgt aaaagttgaa
    20101 caagtgttgt tagacaatga agggcggaaa attacaaaaa acgaaaccac gatagaagtt
    20161 ttacccgatt cgctggctgt ggcttctcaa tcattttcgg tcaataaacc gattctctgg
    20221 gatattgaac atccttattt gtacaaaatg attacaaaac tttcagttga aggcaaggag
    20281 acagatgaat atgaaacatc cgtcggattt cgatatttta attttgatgc tcaaaagggt
    20341 ttttcattaa atggaaaaca tttaaaaatt aacggagttt gtaatcatca cgacttaggc
    20401 gctttgggag ctgccatcaa caaaagggct ttagaacgtc aattgcaact gcttaaagac
    20461 atgggttgca atgctattag atgctcacat aatcctcctg ctcccgaact gttgcagctt
    20521 tgcgacagta tgggattcat tgtaatggat gaaatttttg acatgtggtt caaaaagaaa
    20581 tcggcttatg attatgctca agactttccg aaatggtata aaacagatat gaccgattgg
    20641 ataaaaagag atcgcaatca tccttccgtt tttatgtgga gtattggaaa tgaagtgatg
    20701 gaacaatggg aagacaacat tacgaccgac aacatgaatc ttgatcaagc gaatcttttg
    20761 ctaaacaatt taaaaatatc cgattcccca aaagctaaaa tttcagaaag ttccttgcta
    20821 accaaacatc tttccgatat tgttaagtta ctggatccta caaggccggt aacagcagct
    20881 tgcaatggaa cccaaaaaaa caatccgctt tttttgtctg gaggacttga tattattggt
    20941 ttcaattatc atcacaacga atttatggat gttcctaaaa actttcctgg caaacccttt
    21001 attgttacag aatctgtatc ggcagtacaa acaagaggat tttaccttat gccatccgac
    21061 agcatatata tttggcccga aagttgggat aaaccttttt tcaatccttt gcaaaaatgt
    21121 tctgcctatg acaattctca tgttccctgg ggctctaccc acgaaaaaac atggaaaata
    21181 gttaaaaaac acgatttcat ttctggccaa tttatttgga cgggttttga ctatttgggg
    21241 gaaccgactc cttactcctg gccatcaaga agttcctatt ttggaattat cgatttggcc
    21301 ggcttcccaa aagatattta ttacatgtat caaagtgaat ggacagacaa agatgtttta
    21361 cacgttttcc cacactggaa ttggcaaaaa ggtcaaacga tagacgtgtg ggcttattac
    21421 aataatgccg acgaagtaga actttatctt aatggacgtt ctttaggaaa gagggacaaa
    21481 aagggagacg atttgcacgt tatgtggagg gtaaaatatg aacccggaac gttgaaagct
    21541 atttcacgca aggatggaaa agaagtgctg gcgtatgaag tcaaaacagc cggagccccg
    21601 gtaaaaatcc gcctgacagc cgatagaagc actatctctt ccaatagtaa agatttgtca
    21661 tttgttacag tagagctgct tgacaaagat aataatgttc atccactggc tgatcaattg
    21721 gtgaaattta cgctaaaagg accgggcatg attgccggca ccgataacgg caatgagaac
    21781 gactcaattt cattaaaaaa acctcaacgt catttgttct tcggaaaatg tatggccatt
    21841 gttcaaagca ccgaaaaaaa agggaaaatt acactaaaag caacggtaga tggtttgccg
    21901 gatgaacaaa tcgttattac aagtaaataa agataaactc aaccctattt atagtcattt
    21961 tataaacagg gtttagtttt tccctgaaca ttgtttataa aaaacagagc ttgacatttc
    22021 gaatcaatga aatatgacaa aaaatggata attttgcacc atcagaaaga aaatatttct
    22081 taaaggagaa gggtacattt ggttgatttg taaccgaatt tcatttttta aagattaagc
    22141 atccatgtcg ctggaagatt acatattgca acattcggac agtgaacctg cctatttgac
    22201 aaaaatcagt cgtgatacac atgtcaagct tctgaatccc agaatggttt cgggacattt
    22261 gcaaggcaga gtattgtcca tgttgtgcaa aatgattcaa ccccataaag cgcttgaaat
    22321 aggtactttt accggatatt cggctttgtg cattgcagaa gcattgacgg atgaggatgt
    22381 tttgcatacg atagaaattg atgatgagct ggaagatttg attttggaaa acttcgccgg
    22441 ttcggaacat ggacacagaa ttaagttgca tatagggaat gcactggaaa ttattccgac
    22501 tctgaatgaa gtttttgatt tggtatttat tgatgccgac aaacgagaat acacagctta
    22561 ttttgaagcg gtttttccaa aactcagaaa tggcggtttt attttggccg acaacaccct
    22621 gtggaatgga aaagtcgtta cggaggcaaa acccaacgac agacaaacga tggaaatttt
    22681 gcggtttaac gatttaatcg catccgatga tagaatagaa aaagttatat tgcccttaag
    22741 agatgggtta acgataatca gaaaaaaata actgccatga cttatactta tgaactggaa
    22801 atgaaggtgc gcgactacga gtgcgacttg cagggaattg taaataatgc ggtttatcaa
    22861 aattactttg aacatacgcg gcacgaattt ttgcaggcga atcatctcag tttttcggaa
    22921 ttgcatcagc aaggcatcga tgcagtagtt gcccgcatcg aaattgcatt taaaaaacct
    22981 ttattgccgg gcgaagtatt tctttccaaa ctttatctga gaaaggaagg agtaaaatac
    23041 agtttttatc aggctttatt taaaaaggca gagggaacac tttgcgccaa agcaaaagtt
    23101 gatacagtag tagtgatcaa cggaaaattg gcttcggctt tcgaaccttt tgaaaaactt
    23161 ttggccgaca accatgccgt aaaaggcgaa gagtaaaaaa tttttcttgt gccgacaaca
    23221 atttgacttc tcgaagtgtt tgaaaagctg taggattttt ttattgatgg aacaaacaac
    23281 aaccattaac atgagtgatt ttttggaaac ggaagaaaag atagttcaaa tgctccgaac
    23341 ggtttacgac ccggagatcc ctgtcaatat ttatgacttg ggtctggtgt acaacatcga
    23401 tgttgacgaa gacggacatg tagaaattga tatgactttg acggcaccca attgtcccgt
    23461 tgccgatttt attattgagg atgtccgtat gaaagtggaa agtattgaag gcataaaaga
    23521 tgtggatgta aacattgtgt acgagccggc gtgggacaaa agcatgatga gcgaagaagc
    23581 aaagctggaa cttggattac tgtaaaaaaa atatattttg agaaatattt cacatataaa
    23641 tttcaatcgt aaaactactg cagcgagagg agggaagaaa gtatgattta ttttctttcc
    23701 gatgctcatt tgggttcacg tttggtggaa aatccccgcg agcacgaaca gaagcttgtt
    23761 cgttggttgg agcaggtgcg acgggatgca gaagccattt acctgttggg tgatattttt
    23821 gacttttggt ttgaatacaa aacggtcgtt ccaaaagggt ttgttcgctt tttgggcaaa
    23881 ctggccgaat tgagcgatca gggaattgaa attcattttt ttaccggcaa tcacgatatt
    23941 tggacatttg ggtatttgga ggaggaaatt gggatgaaag tgcatcgtga gcctttgacg
    24001 gtgaaattgg gaaacaaact tttttatctg gctcacggag atggtttgta cacaaatgaa
    24061 cctaaatttc tttttatcag aaaaattttt cacaattcct ttgctcagcg attgtttcag
    24121 ctcgtgccgc cgcgactggg gcagaaattt ggttattggt ggtcgaaaaa aagcagggag
    24181 aaagttctca gcctggataa taaatattta ggagaagaaa atgaaaatct tgtgattttt
    24241 gccaaaaatt atattttgaa tcatgaggtg gattttttgg ttttcggaca tcgacatatc
    24301 gcactcgatt tgcagttgca acaacaaaaa agggtaatta ttcttggcga ttttgtcgga
    24361 attttttctt atggcgtttt cgatggagaa aattttcgac tcgaatattt tgaaccgcaa
    24421 ggagaataat ccgattcatt cctgtttcga cttgaaagcc aacgcataca ttttcatttg
    24481 tttcagcatg gacaacaaac cattggaacg tgttggcgac aaatgttcgc gcaagcctat
    24541 tttatcgatg caatacaaat cggcattcaa aatttcgtcc ggcgtgtgtc ccgacaaaac
    24601 atggatgagc agataaacaa ttccctttac aagtaccgca tcgctatcgc ctcgaaaata
    24661 gatttttcca tccttcattt gtgcacccag ccacacccta ctttgacagc cttcaattag
    24721 attttgaggc gttttatatt cattttcgag tggttccagc gaatttccca tatcgataag
    24781 caaagcatat ttatccatcc agtcgtcaaa ctgactgaac tgttctatta tttcatcctg
    24841 taattcgttg attgtcattt tctttctgat aatttaatgt ttttttggaa aaattttcat
    24901 gaaattgtat ttttttctgc aacatttatt tcagcatatt taccactttt ttgagggctt
    24961 ccgtaaagcg tacgatttct tctttggtat tgtaaaaagc aaatgaagca cgcacggtgc
    25021 cgggaattcc aaacatatcc ataatgggtt gagcgcagtg atgaccggtt cgaacggcta
    25081 ttcccagctt gtcgagcaac atgccaatgt cgtagggatg tattccatcg accagaaaag
    25141 aaagaacgga acatttgttt tttgctgtgc cgataatgtg catattttca atttccatca
    25201 gctgctgttc ggcataatgg agcagttcat tttcataagc ggcaatgttg tcgagtccga
    25261 tgttgttaat atatcgaagc gcttcggcca aagctgttga gccgacataa tcgggtgttc
    25321 cggcttcaaa cttgaatgga agttcattgt aagtggtttt ttcaaaggta acggtagaaa
    25381 tcatttctcc gccaccttga taaggcggaa tggcattcaa ccatttttcc ttgccgtaaa
    25441 gaatgccgat gccggtaggg ccataaattt tatgtcccga aaaaacataa aaatcggcat
    25501 ccaattcccg tacgtcaact gaaagatgtg gaacagcctg cgctccatca atcaaaacgg
    25561 gaatgttttt ttcgtgagca atacgaataa tttccttcac cggattaatg gttcccaata
    25621 cattggaaac atgagccacc gatacgatac gcgttttttc gttcaacaga gaagaatagg
    25681 caaccatgtc gagttctccg ttttcgttga caggaattac gcgcagtttc attcctttgc
    25741 gctcgcaaag catttgccaa gggacaatgt tggaatgatg ttccattgcc gaaacgatca
    25801 cttcgtcgcc aggatgacaa aaagcttcac caaatgaaaa ggctaccaga ttgatggatt
    25861 ctgtggtgcc tcgtgtaaaa ataatttctt cttttttttc tgcatgaaga aattcggcag
    25921 cggtcagccg agctgcttcg tgcgcttctg tggctttcag actcagatag tgaacaccac
    25981 gatgaatatt ggcattcaag tgaaaataag catattcgat tttttctaca acgcaacggg
    26041 gtttctgtgt ggtggcagca ttgtcgaaat aaatcaaatc cttgttataa accttctcgc
    26101 ttaaaatcgg aaaatcggcc ctgatatgtt caatttcttc tttattcatg ttttatacaa
    26161 ctatcggaag tctgtttttg tttttgcaaa attacaaaaa aatgaagaaa cactcttttg
    26221 taaattttgt gagaagtttt tatcaatgag ttttaatttg gactttcaag gaaagtaatc
    26281 tataaacttt aaaaacgaaa tagggttcct ctgtcaataa aagttaagaa acccacatgt
    26341 attgcataaa caaacacgaa tgaatatata atattcagac atgtgggttc catcagacct
    26401 ttaaaaaata aattatattc tgatgaaaat cattaaacat ttggcacaat ctcattttat
    26461 caggccaaac aatcgaatgt tacgcaaaaa caaagatttt ctcaatcaat ggcgaagtaa
    26521 attaacccaa attgtccatt agcatcatca ggtagaaaaa tcgaattgaa aattggtt
//
LOCUS       DAOTAX010000025        25904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1562_length_25904_cov_30.835854, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000025 DAOTAX010000000
VERSION     DAOTAX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25904
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1562_length_25904_cov_30.835854"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /locus_tag="PLM04_04270"
     CDS             <1..2129
                     /locus_tag="PLM04_04270"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HON02328.1"
                     /translation="NGDFFTTSGSYLGMPIGGCMVDAELATMPVARKIFTFNENKLPY
                     VGEMSYSGIIRFNQTDYTLHHVNHLRSTNELVLYNRNNGKYTHTNSYGTEVLVELLEG
                     EHWKTNSPVKAKVTKIEKNKGNMPIPAGQAVLSGHGTSQTILDNLSLNDTVEISIGLK
                     LNNEITAPFTEMIGGDNRKEMLKDGVIETSEVWNELHPRTAIGYSQNKDTVIFCVVDG
                     RGVSAGVTTKQLAILMKSAGAFSAINLDGGGSSCMYVKSFGIMNSPSDGNERAVANGI
                     FAVACAPTDNVIAEIKSYTPIIQLPRYGVIMPKFLGYNQYGVLINTDLKGVTLSCTPD
                     VGQILDDGRFVATGSQGGIVTAHYNNIETKLTIKLINSAEIAFRLDSVLVDQRSNYPI
                     QVQTTIGINTMDVLPAALSWTIRDPEICSINENGQLTGLKNGSTMVIGSLGDFKDSLK
                     VTVQIPTSGRMIGDNFNIENWTLNASTPLNAVLNTQNLPANWTHGAAVNYVYTSTRAP
                     FIKLLRNAPMFGLPDTLKLVVNVGDINISKALLSLRANNASSFVSKEFTVIPKNTDTE
                     LSVAMSELFDATDIAIYPVWFDNINFYLGTQTAGQAYTLALKEIVLCYKGIEISGLPS
                     VLLSQYEVYPNPLEGNTLNIRIKNNDAKLLQAQLFHLNGQCVVSQQLNPMNGIATFKI
                     GKLPAGTYLLKIIHKETSETINLLIK"
     gene            2211..3128
                     /locus_tag="PLM04_04275"
     CDS             2211..3128
                     /locus_tag="PLM04_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02329.1"
                     /translation="MKTFKICLLGLFLIINSLSAQNVNIVKRYKVNGNDIKGSFPVLS
                     PAGDKLLFSSENHKGLWLYDLNSKELITICENEGAGYAPIFNADGTKVFYRKTIYQDK
                     RKFDAIESFDLIKKQKVEMISPQRNLSLLQNYHNGFLVSTDRKLLKATFGKTMNNIPP
                     YVCTEDLKLVVYANGKRTELNPIGENDARYIWVSLSPDSEKILFTVAGKGTFVSDLEG
                     KIVASLGYLNAPVWYDNNFVVGMQDKDDGHFITSSKVLMVSLNGKTKMTLSLDSEIAM
                     YPSASSKGNKVAYNKLEGDIIIVELNVKP"
     gene            3143..5563
                     /locus_tag="PLM04_04280"
     CDS             3143..5563
                     /locus_tag="PLM04_04280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HON02330.1"
                     /translation="MNFIKRYGLLIFLAIFAVSSPSQNLSGIKICVNPGHGGYDSDDR
                     NVVIAPFTSGDPNGFWESKSNLDKGLMLKSMLENAGATVIITRTTNTTADDLPLSQIV
                     AMANSANADYMLSIHSNAGNGIANYVLQLYAGLDPNDTYTYPTPTPFSDESRAISTIV
                     AQNLYSNQITNWSATYAVRGDKTFARTAMGWSDGYGVLRGLAVPGAISEGSMHDYIPE
                     TYRLMNMEYKWLEAWHFFKSFCQYYQAGNIPTGNIAGSVRDSRNIIQESYYKLKDTRD
                     ELLPLNGATLTLLETNETYTTDQLHNGVYMFKNLAPGVYHVKAEANGYHAQIFETTVT
                     ANNTTYLNIDLNKVRNTPPQVISYSPNIAPPDSAECSTDIVLTFNWDMDVTSTTEAFS
                     ITPSVEGTITFEDSQRILRFKPLLPLEKATLYTVRLAKTAKHPDNLSMTEDFTFQFLT
                     KNRNRLKLLAAYPYEGNHKVYTKPQFRFIFDRVLNTSNLQTAVKVLDKNGTELTKNAR
                     SVLNNKVQAPYGSTYFELTNALTPDEEYKVLIAGNVKDEIGMPLVEPIEINFKASNVL
                     VTDQPIVEKFETTNSYSYSNDQSLNVSSASVSRNTSKKLFETSSYLFSYSFSEPEAYA
                     TYLATTLGFVATHDKVVGLHVYGDLSGNSIELRFVAGAEVKYVKLCDLNFFGWEFVET
                     KLDHLQEGLPYEFTGFRVVRNEGILSGNGEIYFDNMLLYDEPIMAIPQVLKNKIQIYP
                     NPASNMVMVKTSDQFISQMQLYSLQGKLLKETNKTNLDISNVAAGTYVLKIKTEKDIV
                     SFPLIIVH"
     gene            complement(5640..6584)
                     /locus_tag="PLM04_04285"
     CDS             complement(5640..6584)
                     /locus_tag="PLM04_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567067.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HON02331.1"
                     /translation="MGMDTGFQQRYLNYNQVLKSEFDERIQKISVHAGFTCPNRDGTK
                     GWGGCTYCNNQTFVPEYCKPVKSITQQLDEGVNFFRHKYKSQYYLAYFQSYTNTYDSF
                     ENLKSLYEEALHHPKIKGLVIGTRPDCVSEDVLDYFERISKNSYVLIEYGIESTSDAT
                     LEFIRRGHDFACAEFAVKETARRGIKTGAHLILGLPFESKEIILEHADKISRLPLTAL
                     KLHQLQIVKGTVMAVQYRQHPEWFQLFTVDEFVDLVVDFLERLSPNIAVERFVSQSPE
                     GLVIAPQWKIKNFEITSKIEKQLIKRNTFQGRCWKGKD"
     gene            complement(6683..8431)
                     /locus_tag="PLM04_04290"
     CDS             complement(6683..8431)
                     /locus_tag="PLM04_04290"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HON02332.1"
                     /translation="MENDALLKEVMSKAQKWLDGNYDENTKNEIRRMMAAEDKTELIE
                     AFYKDLEFGTGGLRGIMGVGTNRMNIYTVGSATQGLSNYLKAQFSDLPQISVVIGHDC
                     RNNSRHFAEISANIFSANGIKVYLFENLRPTPETSFAIRYLGCQSGIIITASHNPKEY
                     NGYKAYWNDGAQMVSPHDVNVIAEVNKITRVEDIKFEGNPELIEIIGEKIDKAFIDAI
                     KTLSLSPEAIRRHSNLKIVYTPLHGTGITLIPRALEEYGFKNIIHVPEQDVISGDFPT
                     VVSPNPEEPAAMEMAVNKAKEVDADIVMASDPDADRMGIAVKNDKGEWILVNGNQTAL
                     LFVYYLIRRWKELGKLTGNEYVVKTIVTTELIKNIADRNGVECFDCYTGFKWIASVIR
                     ENEGKKVYIGGGEESYGFLPEDFVRDKDAVSCCALMAEITAWAKDQGKSLFELLQDIY
                     LEYGYGKEKGISIVKKGKSGADEIQEMMRNFRNHPPKEIAGSKVSIIKDFQTLKMLNL
                     KTNEENSLNMPATSNVLQFFTEDGTKISVRPSGTEPKIKFYIEVRGEMKSRADFDKAE
                     TEALKKIEAVRASLGI"
     gene            8907..9824
                     /locus_tag="PLM04_04295"
     CDS             8907..9824
                     /locus_tag="PLM04_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HON02333.1"
                     /translation="MNIKFHFLLFTAIWLSECLPFPLNAAKPQKETIKILAIGNSFSD
                     DAVEYLDELAIADSVPLIVGNLYIGGCSLERHWKNATELLPDYSYRKSNLGIFTNQDH
                     KTLLDGLLDENWDIVVVQQVSQLSGKYDTYFPYLPNLMKFVKTHAKNPKVKFALHQVW
                     AYAQNSTHEGFANYDRDQMKMYLAITETVKKVAATTGIKIIIPSGTAIQNCRTSEIGD
                     HLNRDGYHLDLHIGRYVAACTWFETFFHRSCVGNPFKPAGVTDRQAKIAQYAAHYAVQ
                     RPFEITLMTNFAWTFLPSSEKTISEFLVY"
     gene            10057..11052
                     /locus_tag="PLM04_04300"
     CDS             10057..11052
                     /locus_tag="PLM04_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HON02334.1"
                     /translation="MDQSVDIRELNERIERESSFVDALVMGMNKVIVGQKHLIESLLI
                     GLLADGHILLEGVPGLAKTLAIKTLSDLIKGNFSRIQFTPDLLPADVVGTMIYSQKKE
                     EFSIRKGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGEKSFKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVIINYPKKDEEKLIIRENLQKNMPSVQPILSPEDIV
                     KARDIVRQIYIDEKIEHYIVDIVFATRFPEEYGLESLKNMISFGASPRASINLALAAR
                     AYAFIKRRGYVIPEDVRAVCYDVMRHRIGLSYEAEANNMTSEEMITEILNTVEVP"
     gene            11172..12050
                     /locus_tag="PLM04_04305"
     CDS             11172..12050
                     /locus_tag="PLM04_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HON02335.1"
                     /translation="METSEILKKVRKIEIKTRGLSQDIFAGAYHSAFKGRGMTFSEVR
                     EYQYGDDIRTIDWNVTARFGRPYIKIFEEERELTVVLLIDVSGSQEFGTVSRMKKDMM
                     TEIAATLAFSTIQNNDKIGVIFFSDKIEKFIPPKKGRKHVLYIIRELIDFRPESNKTN
                     LAGALRYFTNAIKKRSTAFVISDFMDSNFDNELTIANRKHDVVALQIYDERETEMPDI
                     GLVKLKDAETGQRIWIDTADKHLRTFYKHHWGERQLNLQRAFTKAKVDAVSMNTSDDY
                     VQALMRLFQMRSKIGR"
     gene            12069..13067
                     /locus_tag="PLM04_04310"
     CDS             12069..13067
                     /locus_tag="PLM04_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HON02336.1"
                     /translation="MRKRNKHIKLLINLCISLLVSTTLSAQKFSVTVKVDSSVIWIGD
                     QTGFTLEVSQAIGQKVNFPLFSDTLIHGLEIVEPLKIDTVAGDNNSIRVLHKYIITAF
                     IDTLYYIPSYPFVEGTDTVWSNSVSLKVVQPFQIDTASNSITDIKPVFQPKYDWSGLF
                     KKILLTLLVLAVLAAAYYFIRKKIQKKPVILIHKEEPAIPPHEAALHALDKIKEEKLW
                     MKGKYKEYHTQLTDVIREYIERRFLINSMEMTSSEILDNLPKNIYKEQKTAFSALHQL
                     LQLADLVKFAKWVPSPEENISSLNNAYLFVEQTKEETPLTEEKQTEKPVDKKEGEE"
     gene            13120..14103
                     /locus_tag="PLM04_04315"
     CDS             13120..14103
                     /locus_tag="PLM04_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HON02337.1"
                     /translation="MTFHNPGYLFLLLLLLPIIYWYIKEMHKSDASLQISSLQDLTKF
                     PKSKRIKMRHLPFILRLFTLIFLIIAIARPQSSNSLRNETTEGIDIMIALDISGTMLA
                     EDLKPNRLEASKAVATEFILSRPNDNIGLVIFAAESFTQCPLTTDHDVLINLFKGVQY
                     GMIEDGTAIGLGLANAVNRIKDGKAKSKVIILLTDGSNNRGDIAPVSAAEIAKTFGIR
                     VYAIGVGSHGMVRVPVPTPLGMQYQLMESEFDEQTLKAIANITGGKYFRATDNLKLRS
                     IYQEIDQLEKTKISVREYSKKNEMFYLFALAAFLCLISEILLRNTVLRKIP"
     gene            14160..15194
                     /locus_tag="PLM04_04320"
     CDS             14160..15194
                     /locus_tag="PLM04_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HON02338.1"
                     /translation="MFRFAHPEYFFLLIVVPVLIGTFLYTQALKKRRLKKFGDPELLA
                     ELMPNVSYRRPVVKFFLQLFAVILLIIVLAQPQFGSKVENVKRQGIDVMIALDVSNSM
                     MAQDIQPNRLEKAKQILSKLIDQMNDDRIGLIVFAGDAYTQLPITVDYVSAKMFLSAI
                     STNMVPRQGTAIGTAIDMAIKSFGQKSQSGKAIIVITDGENHEDDAISAAKLAAQNGI
                     NVHVIGVGSPEGAPIPIPGTMSFKKDKDGNVVVSKLNEEMCKNIAAAGNGIYVRADNT
                     NNAYRIISKQLDTLAKSTTESKVFSDYSEQYQSFALIALIVLIIDFFLFDRKNKKLSK
                     IKIFDIENEK"
     gene            15184..15921
                     /locus_tag="PLM04_04325"
     CDS             15184..15921
                     /locus_tag="PLM04_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02339.1"
                     /translation="MKSKFQNFSVKTNVHSEFKKGVFLFLIMSVLSVSVSAQKGNNEV
                     RAGNKKYNNKKYIEAEIDYRKGLEKNPKSFEGNYNLGNSLFRQKKYEEAYQQYEKAAS
                     YAPKTEKEKLAATYHNMGNSLLMQKKIEESIEAYKQALRHNPKDNDTRYNLAYAQSLL
                     KKQQQQQNKNDKNKDQKQDQNQDNQQNKQNDNQKDQKNQQQQQQQQMTKENAQQILDA
                     LSQDEKETMNKAKKLPSKQEQKPDKDW"
     gene            16015..>17053
                     /locus_tag="PLM04_04330"
     CDS             16015..>17053
                     /locus_tag="PLM04_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HON02340.1"
                     /translation="MKRKIFLLFLIFAGFIGVKADEPVRFTATAPSTVILDKPFQLVY
                     TINAQGKDLRIPEIKDFDILAGPFESRSSSIQIVNGKRSSSMSISYTYTLMPQRTGTF
                     TIPSASIMVNNQKYTSNGLTIKVLPADQQSDESSSPADNGRKSSPKNISNENIFIRTI
                     PSKTSVYEQEALLITYKLYTLVDVAQCVNKKMPDFDGFLKQEIEQTQNKQFSYENYNG
                     RNYGTVVLYQTLLYPQRAGTIQIDNAVFESVIRVQNQTSIHSIFDDFFDSYTNVTKNL
                     VAPGLKITVKPLPEDKPMVFGGAVGNFTMSSSISAQEVKENEAVTLKINIAGSGNMKL
                     IKTPEVKFPSDF"
     assembly_gap    17054..17153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17154..17993
                     /locus_tag="PLM04_04335"
     CDS             <17154..17993
                     /locus_tag="PLM04_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HON02341.1"
                     /translation="SGNMKLIKTPEVKFPSDFEIYDPKVTNNFKTSASGVSGTKTIEY
                     LFIPRHSGNFEIPPVEFSYFDLRDKTYKMLRTPSYSLKVLKGSGNQNATVVSNYNPKE
                     DIRQINKDIRYIYTGNIRLIKPSTPVFGTTLAWLLFIIPLVVVVGLFFFFRKKVKENA
                     DIALVRNKKANKMAQKRLKLAQKLLKEGNKDAFYDEILKATWNYLSNKLSIPVASLTR
                     ETVSEELKLRGVTDELTKKLMKILNDCEFARYAPKSGQQEMGNIYEETIQVISELEEA
                     IKK"
     gene            18103..18861
                     /locus_tag="PLM04_04340"
     CDS             18103..18861
                     /locus_tag="PLM04_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02342.1"
                     /translation="MKRLISNIILIITLLASLPLSAQSDAVKYANDLYAKGNFHAAAE
                     QYEKILKNEGIAPELYYNLGNAYYKMNEIGLAILNYERALKLSPRFEDARFNLQLAQQ
                     KVLDNVNYNQQFFIQRWITNLMHMFTSNQWFTAGYLFFLAALIFAFLFAFGSSVRQRK
                     NSFYIGIVLLLFAVTSLIFSGIRKNEETNSLQAIIMTGVVTVKSSPDESGTDLFQLHE
                     GTKVTIKSTLGNWAEIKIGNGNVGWVESKSIEKI"
     gene            18902..19585
                     /locus_tag="PLM04_04345"
     CDS             18902..19585
                     /locus_tag="PLM04_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HON02343.1"
                     /translation="MNLIETLRIWDTNLFLALNGIHSPFFDGFMMAFSNKLVWIPFYI
                     SVLYVMIKQWKSEAFWIVLSFAVCILLADQISSGIIKNAVQRLRPSREETISGLVHLV
                     DGYRGGKYGFVSSHAANSLGFAVLSAYFFRKRSYTLIILLWSLITGYSRIYLGVHYPL
                     DVMGGYFVGFFVAIFCYWVLKKLKPNIFYVSSRFRSKRFADSALPLLVLTFTVVGIFV
                     WSVIRILAF"
     gene            19673..19745
                     /locus_tag="PLM04_04350"
     tRNA            19673..19745
                     /locus_tag="PLM04_04350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19706..19708,aa:Thr,seq:cgt)
     gene            19710..20123
                     /locus_tag="PLM04_04355"
     CDS             19710..20123
                     /locus_tag="PLM04_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02344.1"
                     /translation="MNRSGVRVPKKALYNDFQKMMFDNILFASFVPQILMILGYIMCL
                     SAATSSSHVSGISEITPITQHQVFFSTENKIETENTFQFEDYFFVSAIIPSKESIKPI
                     YYFPTIIPLVKCNACISNGFLAELFCRPPPTLSFC"
     gene            20391..22649
                     /locus_tag="PLM04_04360"
     CDS             20391..22649
                     /locus_tag="PLM04_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02345.1"
                     /translation="MKKILILLSLVPTFIFAVDLEPKDSLKHVYLEEIQIISHKETLP
                     AQPAASYSVVSSNLINQAQVNSVRDLSILVPNFFIPDYGSSMSTSPYIRGVGARSTGQ
                     SIALYMDNVPYFEKSTFDFDFYDIQQAEVLRGSQSTLYGRNALGGVVNIYTLSPFEYQ
                     GTKLSVTGGNYGLLQAQASHYAKLSNKTGLSLSGYYHHRNGFFTNTFTGKDADWKNSA
                     GGRAKFDWHITPSLRAQYIINYDYVNQNAFPYGLYNATTKSTAEPNFNDKSSYLRNML
                     TNSVLIEYRNDKIQLTSTTSYQYFSDKMFMDQDFTPLSYFSLEQQQKQHAVNEEIVLK
                     SRTSGNYQWSFGATGFYQKLNTDAPVAFKQDALTNILQPVLDRAHTAGAPQMTITSTV
                     MDIPGTYDTKTAEGALYHQSTFNHLLTDGLSATIGVRADFEKVNLDYFTNTRLDLLMK
                     MGNRNIPTSLADTLRGAESVKFSEFLPKVSVKYAWNSQQYIYANVSKGYKAGGYNIQM
                     FADLIQNDLMNSGNSSASQPDVRKAILFQPEYSWNYEAGCQSTFLDNRLKMQLSLFYM
                     QITDMQLTRFVPSGNGRMITNAGKVDSKGMEFSLQANLGKGFAAGLNYGYAHAAFTNY
                     TDSVKSNGTVNEVDYKGKFVPYAPQNTLNIGGEYLRIFKNSFIDQFMVSMQYNAAGKI
                     YWDVDNSIYQDFYGLINAKVGVEKGNFRVELWAKNLFDTQYNAFYFESFENKFFQKGN
                     PAQFGLTLKTEF"
     gene            22805..23611
                     /gene="mazG"
                     /locus_tag="PLM04_04365"
     CDS             22805..23611
                     /gene="mazG"
                     /locus_tag="PLM04_04365"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HON02346.1"
                     /translation="MEKTTHTMEEKLQEFKRLLEIMERLRAECPWDREQTFESMRVQT
                     IEETYELADAIMKDDKKAIMTEVGDLLLHVVFYAQMGSETGDFDIFDVCKNLNEKLIF
                     RHPHIFSNTKADNSQTVVQNWEDLKLKEKNGNRTVLAGVPETLPALIKAHRIQDKARN
                     VGFDWEKREQVWDKVQEEFQELKDEVEAMDKNKMEEEFGDLLFSLINAARLYDINPEN
                     ALERTNRKFIQRFNYLESQTISQGKDLRQMSLDEMDVFWEEAKKKEKQEG"
     gene            complement(23711..25591)
                     /locus_tag="PLM04_04370"
     CDS             complement(23711..25591)
                     /locus_tag="PLM04_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02347.1"
                     /translation="MFIAIFILISISTFSLKAQLFRDSILKNEEKTIYMNEVIVTGTR
                     SEADVRNLPMSLSVITDKQILDRQSQSVIPLLNELVPGLFITGRGIMGYGISTGAAGT
                     MKMRGIGGNPTTGVLILIDGEPQFMGLMGHPIADLYQSVIADRIEVVRGPASALYGSN
                     AMGGVINIITSKLYKDTVQQNFHFGYGSYSTLESSASNHFHKNRITGKFAISYNRTDG
                     HRENMDFDQISGYAKLGYRLDRYWSVSTNANISHFNASNPGTIDQPIVDNDSHVKRGW
                     INASVENRYRYTSGALSFFYNWGNHIINDGYNMGGQPLDYLFHSNDKISGITFYQSAI
                     LMPNNKITFGFDYQKFGGKAWNKYNDGNKKSIIDTTLYEIAGYADMRQDFGLLATVDI
                     GIRLDNHSKTGSQWIPQVGLSFHLPKNIEAKTIVGKGFRNPTIRELFMFTPQNPNLLP
                     EEVMNYEFSVSQNMVEKNLFYDVAVFYLEGKNMIQTVISNGRPINVNTGKIKNFGFES
                     VITYQINSIWSANVNYSWLDMKYPVTGAPKQKVFAGFDYSKKKLKFSSGIQYVHRLYS
                     SLNPVKKENFTLLNARTTYQLGKFTQLFLTGENLLNCSYEINAGYPMPKATFLIGCNV
                     HF"
     gene            25807..25904
                     /locus_tag="PLM04_04375"
                     /pseudo
     CDS             25807..25904
                     /locus_tag="PLM04_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase"
BASE COUNT         8338 a         5018 c         4827 g         7621 t
ORIGIN      
        1 ccaatggcga cttttttacc acttcaggca gctatcttgg tatgccgatt ggcggttgta
       61 tggtagatgc cgaattggca accatgcctg tggccagaaa aatttttact ttcaacgaaa
      121 acaaattgcc ttacgttggc gaaatgagtt atagtggaat aattcgtttc aatcaaacag
      181 attatacgct ccatcacgtt aatcatctcc gttctacgaa tgagttagtt ttatataaca
      241 gaaacaatgg aaaatataca cataccaatt cttatggtac agaagttttg gttgaattgc
      301 tcgaaggtga acattggaaa actaacagcc cagtaaaagc aaaagttacc aaaattgaaa
      361 agaacaaagg aaacatgcct attccagcag ggcaagccgt tctatccggg catggtacct
      421 cccagacaat attggataat ctgtctttga atgatacagt ggaaatttct attggcctga
      481 aattaaataa cgaaataacc gcaccattca cagaaatgat tggcggagat aatcgaaaag
      541 aaatgttaaa agatggcgta attgaaactt cagaagtttg gaatgaactt catcctcgaa
      601 cggcgatagg atattcacaa aataaagaca ccgtgatttt ttgtgttgta gatggtcgtg
      661 gcgtttctgc cggagttaca accaaacaac tggccatttt aatgaaaagt gcaggcgctt
      721 tttctgccat taatctcgat ggcggtggtt caagttgcat gtatgtcaaa tccttcggta
      781 ttatgaactc tcccagcgac ggcaacgaac gtgcggttgc caatggaatt tttgccgtag
      841 cctgtgcccc aaccgataat gtaattgcag aaatcaaatc ctacacgccg attattcagt
      901 tgccccgata tggagtaatt atgcccaaat ttctgggata taatcaatat ggcgttctga
      961 taaataccga cttgaaagga gtaaccttga gttgcacgcc ggatgtcggt caaattttgg
     1021 atgatggaag atttgttgca accggttctc aaggaggaat tgtaacggct cattataaca
     1081 atatcgaaac aaaattaacc ataaaattaa taaactcagc tgaaatagca tttcgactgg
     1141 attcggtttt ggtagatcaa cgcagcaatt atcccattca ggttcaaaca actattggaa
     1201 taaatactat ggatgttctt cctgctgcct tgagctggac cattcgtgat cctgaaattt
     1261 gttccatcaa cgaaaatgga caattgacag ggttgaaaaa tggcagcact atggtcatcg
     1321 gcagtttggg cgattttaaa gactctctca aggtaacagt tcaaattcca acttcaggaa
     1381 gaatgattgg cgataacttc aatatagaaa actggacatt gaatgcttcc actcctttaa
     1441 atgcagtact caatacgcaa aacttgcctg caaactggac gcatggagct gcagtcaatt
     1501 atgtatatac ttcaacaaga gctcctttta tcaaacttct aagaaatgct cccatgttcg
     1561 gattaccaga tacattaaaa ctggttgtca atgttggaga tataaatatc tcaaaagctc
     1621 ttttgtctct cagagctaac aacgcttctt catttgtatc gaaagaattt actgttattc
     1681 caaaaaatac tgacactgaa ctttcagtag ccatgagcga acttttcgac gctacagata
     1741 ttgccattta tcccgtctgg ttcgacaaca tcaacttcta tctcggtaca cagactgcag
     1801 gacaagctta tacgctggct ctcaaagaaa ttgttctttg ctataaagga atagaaattt
     1861 cagggctgcc ttctgtcttg ctttctcaat acgaagttta tcccaatcca ctagaaggga
     1921 atacgttgaa cataagaata aaaaataatg acgcaaaact tctgcaagct caactctttc
     1981 atttaaatgg tcaatgcgtg gtttctcaac aactaaatcc aatgaatgga atagctactt
     2041 ttaaaattgg caaattaccc gctggaactt atttgttgaa aattattcat aaagaaactt
     2101 cagaaacaat aaatcttctg atcaaataga atcttattca ctatcataat aaatttaaca
     2161 atttcaaata tttcaattca ctattataat cattttaaaa acagaatcca atgaaaacat
     2221 tcaaaatctg tttactgggg ctatttttga taataaactc attatctgct caaaatgtaa
     2281 acattgtgaa gcgctacaaa gtgaatggaa acgacatcaa aggttcgttt ccggtattaa
     2341 gccctgccgg agataagctc ttgttctcat cggaaaatca caaaggattg tggctgtacg
     2401 acttaaactc taaagaattg attactattt gtgaaaatga aggagcaggt tatgctccga
     2461 tatttaatgc tgatggtaca aaagtttttt acagaaagac tatttatcag gacaaacgaa
     2521 aatttgatgc catcgaaagt tttgatttaa taaaaaaaca aaaagtcgaa atgatttcac
     2581 cacaaagaaa tttgtctctt ctgcaaaatt atcacaacgg atttttggta tctaccgacc
     2641 gaaaactatt gaaagctact tttggtaaaa caatgaataa tatacctccc tatgtttgta
     2701 cggaagactt gaaactggta gtttatgcca atggaaaaag aacggaatta aatccgattg
     2761 gagaaaacga tgccagatac atttgggttt cactctctcc cgatagcgaa aaaattctct
     2821 ttaccgtcgc aggaaaaggg acttttgtat ccgatttgga aggaaaaatc gttgcttcgc
     2881 tcggatacct gaatgcgccg gtttggtatg acaataactt tgtagtgggc atgcaggaca
     2941 aagatgatgg acattttata acttcctcga aagtgctaat ggtttcgctg aacggtaaaa
     3001 ctaaaatgac cttgagcctc gatagcgaaa ttgccatgta tccttcagct tcttctaaag
     3061 gaaataaagt tgcctacaat aaattggaag gagatatcat aatagtggaa ttgaatgtaa
     3121 aaccataaat cagtaaaaaa tcatgaattt tatcaaaagg tatggcttgc taattttttt
     3181 agcgattttt gccgtttctt ccccatccca aaatttatca ggtataaaaa tatgtgttaa
     3241 tcccggtcat ggcggttacg acagcgatga cagaaatgta gttattgctc ctttcacttc
     3301 aggcgatccg aacggatttt gggaatcgaa aagcaatttg gacaaaggat taatgcttaa
     3361 atcaatgttg gaaaatgctg gtgcgacggt tattattacc cgaaccacca atactacagc
     3421 agacgattta ccattatctc aaattgtagc tatggcaaat tcggcaaatg cagactacat
     3481 gctttctatc cacagcaatg cagggaatgg tattgcaaac tatgttctac aactttatgc
     3541 gggcctcgat cctaacgata cctataccta tcctacaccc acaccttttt cggatgaaag
     3601 cagagccatc agcacaattg ttgcacaaaa tctttactca aaccagataa ccaactggtc
     3661 ggcaacttat gcagtgcgtg gtgataaaac atttgcacgc acagctatgg gttggagcga
     3721 cggatatggt gtgctgcgtg gattagctgt tccaggagct atttctgaag gatctatgca
     3781 tgactatatt cctgaaacct ataggcttat gaatatggaa tataaatggc ttgaagcctg
     3841 gcatttcttt aaatcctttt gtcagtacta tcaggcaggg aacatcccga caggaaacat
     3901 tgccggctcc gttcgcgaca gtcgaaatat aattcaggaa agctattata agctgaaaga
     3961 caccagagat gagttgcttc ctctaaatgg tgcaacatta acacttttgg aaacaaatga
     4021 aacttataca acagatcaac ttcacaatgg agtttatatg tttaaaaatc tcgctccggg
     4081 agtttatcat gtaaaagcgg aagccaatgg ttatcatgct caaatatttg aaactacagt
     4141 tacagcaaat aataccacat atttgaatat agatttgaat aaagtaagaa atactccacc
     4201 acaagtaatt tcttactctc ctaatattgc tcctcccgac agtgccgaat gttctaccga
     4261 tattgttttg actttcaatt gggatatgga tgtaacttca acaacagaag ctttttcgat
     4321 tacgccgtca gtagaaggaa ccatcacttt tgaagacagt cagcgcattt tgcgattcaa
     4381 accattgctc ccgcttgaaa aagcaacgct ttacactgtt cgattggcaa aaactgctaa
     4441 acaccccgac aatttaagca tgactgaaga ttttactttc cagtttttaa ccaaaaatag
     4501 aaatcgttta aaattacttg ctgcttatcc ctatgaaggt aatcataaag tttatacaaa
     4561 acctcaattc agattcatat tcgatagagt tttaaatacc agcaacttac aaactgctgt
     4621 aaaagtacta gacaaaaatg gaactgaatt gactaaaaat gcacgtagcg ttttaaataa
     4681 taaagttcaa gctccttatg gttctaccta ctttgaacta accaacgctt taactccaga
     4741 tgaagaatat aaagtgttga ttgcaggcaa tgtaaaagat gaaattggca tgcctttggt
     4801 ggaaccaata gaaatcaatt tcaaagcttc taatgtttta gttacagatc aacctattgt
     4861 tgaaaaattt gaaacaacaa atagttactc ttattcaaat gaccaaagtt taaatgtttc
     4921 ctctgcttct gtgtctcgta acacttctaa aaaacttttt gaaacttcct cttatttatt
     4981 cagctacagt ttttcagaac cagaagccta tgcaacttac ctggcaacaa cacttggatt
     5041 tgttgctaca catgataagg tagtcggcct tcatgtgtat ggagatttat cgggaaactc
     5101 gatagaactt cgatttgttg caggtgcaga agtcaaatat gttaagttgt gtgatttgaa
     5161 tttctttggt tgggaatttg tagaaactaa attagaccat ttacaagaag ggctgccata
     5221 tgaatttaca ggttttagag tagttagaaa tgaagggatt ttgtctggta atggagaaat
     5281 ttattttgat aatatgcttc tttatgatga gccaataatg gctattccgc aagtattgaa
     5341 aaataaaata cagatttatc cgaatcctgc ttcaaacatg gtaatggtta aaacttccga
     5401 ccaattcatt tctcaaatgc agctttattc tttgcaagga aaattattga aagagacaaa
     5461 taaaacaaat ttggatatca gcaacgtcgc tgccggaact tatgtgctga aaataaagac
     5521 agagaaagac atcgtcagtt ttccgttaat aatcgttcac taaaaaaaca caaatatcag
     5581 tgaagagctt acttattatg tacactcttc actgatattt atattataat cctatttttt
     5641 caatcttttc ccttccagca tcttccctga aaagtatttc gcttgatgag ttgtttctct
     5701 atttttgagg ttatttcaaa attttttatt ttccattgcg gggctatcac caacccttcg
     5761 ggcgattgag aaacaaatcg ttcgacagcg atgtttggag aaagtctctc cagaaaatct
     5821 accaccaaat cgacaaattc gtcaacagta aaaagttgaa accactcggg atgttgacga
     5881 tattgaaccg ccatcactgt tcctttcact atctgaagtt gatgaagttt caatgcagtc
     5941 aaaggcaatc gggaaatttt atcggcatgt tcaagtatta tttccttact ttcaaatgga
     6001 agtcccagaa tcaaatgagc accggttttg atgcctctgc gagcagtttc cttcaccgca
     6061 aattcggcac aagcaaaatc atgcccgcga cgaataaatt ccaatgtagc atccgaagtt
     6121 gattcaatgc cgtactcaat aagaacatag ctgtttttag aaattctttc gaaataatcc
     6181 aatacatctt cactcacaca atcgggacga gtgccgatta ccaaaccttt tatttttgga
     6241 tgatgcaaag cttcttcgta cagcgatttc agattttcaa aactatcgta tgtatttgta
     6301 tatgattgaa aataagctaa ataatattga cttttgtatt tatgccgaaa aaaatttact
     6361 ccttcatcga gttgttgggt gatacttttc accggtttac agtattcggg tacaaacgtc
     6421 tgattgttgc agtaagtaca gccaccccac ccttttgtcc catctcgatt gggacaggtg
     6481 aatccggcat gaacggaaat tttttgaatg cgttcatcaa attctgattt caaaacctga
     6541 ttgtagttta aatatcgttg ctgaaagccg gtatccattc ccattctgct aaaattttgc
     6601 atgaaaggta aaaggtaaaa aagcgtcatt cgttaataat tatctagaat gacgctttca
     6661 atattctaaa aaatcaatat cgttaaattc ctaacgaagc gcgaacagct tcaattttct
     6721 ttaaagcttc agtttcagcc ttgtcaaaat cggctcgaga tttcatttcg cccctaactt
     6781 caatataaaa tttgatttta ggctctgtac ctgaaggacg aaccgatatt ttggtgccat
     6841 cttccgtaaa aaactgcaat acattcgatg tggcaggcat attcaatgag ttttcttcat
     6901 tggtttttag atttaacatt ttcaaagttt gaaaatcttt gataatagaa acctttgagc
     6961 cggcaatttc tttgggagga tgatttctga agttgcgcat catttcctga atttcatccg
     7021 cacctgattt tcctttttta acaatggaaa ttcctttttc ttttccgtat ccgtattcca
     7081 ggtaaatatc ctgcaaaagt tcaaaaagag attttccctg atcttttgcc catgcagtaa
     7141 tttcagccat caaagcgcag caggaaacgg catctttgtc gcgaacaaaa tcttccggca
     7201 agaaaccgta actttcctct cctccaccaa tgtaaacttt tttgccttca ttttcgcgaa
     7261 tgacggaagc aatccattta aaaccggtat agcagtcgaa acattctact ccgttccgat
     7321 cggcaatatt tttgataagt tctgttgtta caatcgtttt tacgacatat tcgttgcctg
     7381 tgagttttcc caattctttc cagcgccgaa tcaggtaata cacaaacaaa agagctgtct
     7441 gattgccatt gaccaatatc cattcgcctt tgtcgttttt tacggcaatt cccattctgt
     7501 cggcatccgg atcggaagcc atcacaatat cggcatctac ttctttggct ttgttgacag
     7561 ccatttccat cgcagccggt tcttcgggat ttggagaaac aacagtagga aaatcaccac
     7621 taattacatc ctgttccggc acatgaataa tatttttaaa accgtattct tccaaagctc
     7681 gcggaattag agtaattccc gttccgtgaa gtggcgtata aacgattttc aaattgctat
     7741 gccgacgaat agcctctggc gacagggaaa gagttttaat tgcgtcgata aaagctttgt
     7801 cgattttttc tccgatgatt tcaatcaatt cgggattgcc ttcaaatttt atgtcttcca
     7861 cccgagtgat tttattgact tcggcaatta cgttcacatc atgaggtgaa accatctgtg
     7921 cgccatcgtt ccaataggcc ttgtaaccat tatattcttt cggattgtgc gaagctgtaa
     7981 taataattcc actctgacaa cccaaataac gaatggcaaa agaagtttcg ggcgtaggcc
     8041 ttaagttttc aaacaaataa accttaatgc cgtttgccga aaaaatattc gccgaaattt
     8101 cggcaaaatg acggctgttg ttgcggcaat cgtgtccaat tacaacactg atctgaggta
     8161 aatcggagaa ttgagctttt aaataattac ttaagccttg cgtggctgat cctacggtat
     8221 aaatattcat ccgattggtt cccacgccca taatgccacg caagccaccg gtaccaaatt
     8281 ccaagtcttt atagaaagct tctattaact ctgttttgtc ttcggcggcc atcattctgc
     8341 ggatttcatt tttggtattt tcatcataat ttccatccag ccatttttgt gctttcgaca
     8401 tcacttcctt taataatgca tcattttcca tatttttata tttttgatgt taacttttta
     8461 atttctaaaa ttcaaaaacg cttctgcccc acttgttttt tgtttctgtt tcgaacaaat
     8521 tttaaatgaa atttttaaat caggaaatta cctgcagatt aaaatatcaa acaaattcgg
     8581 cttttctccg acaaaaataa taaatttagg tgttaggtca aaaaatagac tgtttttaaa
     8641 actaatagat tcaactatca agtcgaaatt gcattaataa tttcatgaac tcatttttaa
     8701 gtagtgggta attaaaatca aatttttctc gataaattcg atgttaagtt caaacttgac
     8761 catcttttct ctataaaaaa gtgaaaaatc cgctttggcc attaaattag attaaaacaa
     8821 gactatttcc accaaaggct ctgcatgaaa atgctatttt tgcagtaaag aaagttttca
     8881 ctatcaacga aaaacaccaa accaccatga atataaaatt tcacttctta ttgtttaccg
     8941 ctatttggct gagcgaatgc ctgcctttcc cattgaatgc cgctaaaccg caaaaggaaa
     9001 ccattaaaat tctggccatc ggcaacagtt tttccgatga tgccgtagaa tatttggatg
     9061 agctggcgat agccgacagc gttccgctga ttgttggcaa tttgtacatt ggtggttgtt
     9121 cgctggaaag gcactggaaa aatgctacgg aactccttcc cgattattct taccgcaaaa
     9181 gcaatttggg cattttcacc aatcaggatc acaaaacgct gttggatgga cttctcgacg
     9241 aaaattggga tattgtggta gtgcagcaag tcagccaact ttcggggaaa tacgatactt
     9301 atttcccata tctgccaaac ttgatgaaat tcgtcaaaac acacgccaag aatccaaaag
     9361 taaaatttgc attgcatcag gtttgggcat acgcacaaaa ttctacccat gaaggttttg
     9421 ccaattacga cagggatcaa atgaaaatgt atctggccat caccgaaaca gtgaaaaagg
     9481 ttgcagccac tacggggata aaaataatta ttccttccgg aacagccatt cagaattgca
     9541 gaaccagcga aattggcgac catctcaatc gcgacggtta tcacctcgat ttgcatatcg
     9601 gccggtatgt tgcagcttgc acatggttcg aaaccttttt tcatcgaagc tgtgtcggta
     9661 accctttcaa gcctgccggc gtaaccgacc gacaggcaaa aatagctcag tatgcagctc
     9721 actatgccgt tcagcgtccc tttgaaatta cattgatgac caattttgca tggacttttc
     9781 tcccatcttc agagaaaaca atttcggaat ttttggttta ttaagttaag aaaaatttca
     9841 atttattatt tccactatct gtacagttga aaagtgaaaa atatcaataa gccaactatg
     9901 agaagaataa agcatttttc actgcaaaat agcgctaaga aggatattac ttaatcatct
     9961 aaattcaggt taaaagttat taaatcagat ttttaacgtt tcaaatagct gtatatttgc
    10021 tcaacaaatt caataaacaa acaaagaaaa aacaatatgg atcaatcggt tgatataagg
    10081 gaacttaacg aacgtattga acgggaaagc tcatttgtgg atgctctcgt tatgggaatg
    10141 aacaaggtaa ttgtaggaca aaaacatttg atcgaatcat tgcttatcgg tttactggct
    10201 gacgggcata ttttactgga aggggtaccc ggccttgcca aaacgctggc catcaaaaca
    10261 ctttcggact tgattaaggg aaatttcagc cgaattcagt ttacacccga cctgctgcct
    10321 gccgacgttg tcggaacaat gatttacagt cagaaaaaag aagaattcag cattcgtaaa
    10381 ggccctattt ttgccaacct cgttttggcg gacgaaatca acagagctcc ggccaaagtg
    10441 caaagtgcct tgctcgaagc catgcaggaa cggcaggtaa cgattggcga aaaatctttc
    10501 aaattggaag aacctttttt ggtgatggcc acacaaaatc ccatcgaaca ggaaggaaca
    10561 tatccgcttc ccgaagcgca agtcgatcgt ttcatgctga aagtcatcat caattatccg
    10621 aaaaaagatg aagaaaaact gattattcgc gaaaacctgc aaaaaaacat gccttccgtt
    10681 caacccatac tgagccccga agacattgta aaagcgcgcg atattgtgcg acaaatctat
    10741 atcgatgaga aaatagaaca ttatattgtg gatattgtgt ttgctacccg attcccggaa
    10801 gaatacggat tggaatcgct caaaaatatg atttcgttcg gtgcatcacc aagagcttcc
    10861 atcaatttgg cgttggcagc acgtgcttat gcctttatca aacgacgcgg atacgtcatt
    10921 cccgaagatg ttcgtgccgt ttgctacgac gtaatgcgcc accgcatcgg tttaagttat
    10981 gaagccgaag ccaacaatat gacttccgaa gaaatgatta ccgaaatttt gaatacggtt
    11041 gaagttccat aaaaaacggc tttgtcatta agagtgttta aaaatgttcc accggagaca
    11101 gaaaagagaa aaatatgcag ctaaaactgt cggaaaaaag tggagaaaat tttccatcta
    11161 cacgttaatt tatggaaacg agtgaaattt taaagaaagt tcgaaaaatt gaaataaaaa
    11221 cccgcggatt gtctcaagac atttttgcgg gcgcttatca ttccgctttt aaaggtcgag
    11281 gaatgacctt ttccgaagtg cgtgaatatc aatatggcga cgatattcgt accatcgact
    11341 ggaatgttac tgcccgtttt ggacgcccct acattaaaat ttttgaagag gaacgcgaac
    11401 tgacagttgt tttgctcatt gatgtttccg gcagtcagga atttggaaca gtttcgcgca
    11461 tgaaaaagga catgatgaca gaaattgcag ctacattggc tttttcaaca attcagaaca
    11521 acgataaaat tggcgtgatt tttttctccg acaaaataga aaaattcatt cctccgaaaa
    11581 aaggacgaaa acacgtactt tacatcatca gagaactgat cgatttccgc cccgaaagca
    11641 acaaaacaaa tctcgcagga gctttgcggt attttaccaa tgccataaaa aaacgctcga
    11701 ccgcttttgt catttccgat ttcatggaca gcaattttga caatgaactg accatagcca
    11761 accgtaaaca tgatgtagtt gcactgcaga tttacgacga aagagaaacg gaaatgccgg
    11821 acatcggact cgtaaaacta aaggatgcag aaaccggtca acgtatctgg attgatacgg
    11881 ccgacaagca tttacgaaca ttttacaaac accattgggg agaaagacaa ctgaacttgc
    11941 agcgtgcatt taccaaagca aaggtagatg ccgtctccat gaatacttcc gacgattatg
    12001 tacaggcgct catgaggctg ttccagatga gaagtaaaat tggacgttaa tacggaattt
    12061 tttaaaaaat gcgaaaaagg aataaacata taaagctgct gataaacttg tgtatcagtt
    12121 tattggtttc cactaccctg tcggctcaaa aattttcagt tactgttaaa gtcgattctt
    12181 ccgtcatttg gattggtgat caaacaggtt ttacgttgga agtatctcaa gctatagggc
    12241 aaaaagttaa ctttccgctc ttttcggata cccttattca cgggttggaa attgttgaac
    12301 cgctaaaaat agatacggtt gcaggagaca acaattcaat tcgcgttttg cacaaataca
    12361 ttattacggc ttttatagat accctctatt atattccttc gtatcctttt gttgaaggta
    12421 cagatacggt ttggtccaat tctgtgtcgt taaaggtagt gcagccattt caaatcgata
    12481 cggcttccaa ttccataaca gacattaagc ccgtttttca gccaaaatat gactggtcgg
    12541 gattattcaa aaaaatactg ttgacattgc tggtgcttgc tgtgctggca gcggcttatt
    12601 atttcatcag aaaaaaaatc cagaaaaaac ctgtcatact cattcacaag gaagaaccgg
    12661 ccattccgcc acacgaagca gctctccatg ctctcgacaa aatcaaggaa gaaaagcttt
    12721 ggatgaaggg aaaatacaaa gaataccata cccagctgac ggatgtcatt cgggaataca
    12781 tcgaaagacg cttcctgatc aacagcatgg aaatgacttc cagcgaaatt ttggataatt
    12841 tgccgaaaaa tatttataaa gaacaaaaaa cggcattctc agctttacat cagcttttac
    12901 agcttgccga tttggtgaaa tttgccaagt gggttccatc gcccgaagaa aacatttcga
    12961 gtttgaacaa tgcctatttg tttgtggaac aaaccaaaga agaaacgcca ctcacagaag
    13021 aaaagcaaac cgaaaagcct gtggataaaa aagaaggcga agagtaaaga taaagcaaaa
    13081 aaatttggtt aaacagataa aaaacgctta caataaaaaa tgacttttca taatccggga
    13141 tatttatttc tcttgttgct gttgctaccg attatttatt ggtacattaa ggaaatgcac
    13201 aagtcggatg cgagtctgca aatatcttca ttgcaagatt tgacaaaatt tccgaaaagt
    13261 aaaaggataa aaatgaggca tttacctttt attttgcgtt tatttaccct catattcctg
    13321 attattgcca ttgcccgacc tcaatcctcc aatagtttac gaaacgaaac aacagaaggc
    13381 atcgacataa tgatagccct cgatatttcg ggaaccatgc tggcagaaga tttgaaaccc
    13441 aatcgattgg aagcatcaaa agccgtagct acggaattta ttctttcccg cccgaacgac
    13501 aacatcggat tggtgatttt tgcagccgaa agttttaccc aatgtccgct cactaccgat
    13561 cacgacgtac ttatcaacct ttttaaaggt gttcagtacg gaatgataga agatggaaca
    13621 gccatcggac ttgggttggc caatgccgtg aatcggataa aagatggaaa ggccaaatcg
    13681 aaagtcatta ttttgttgac ggacggctca aacaatcgag gcgacattgc tccggtttcg
    13741 gctgccgaaa ttgcaaaaac attcggtatc agggtttatg ccatcggtgt tggttctcac
    13801 ggaatggtaa gagttccggt tcctactccc ctcggcatgc aatatcagct tatggaatcg
    13861 gaatttgacg aacaaacttt aaaagctatt gctaatatta ccggaggtaa atattttcgt
    13921 gccaccgaca acctgaaact tagaagtata taccaggaaa tagatcaatt ggaaaagaca
    13981 aaaatcagtg ttcgcgaata cagcaagaaa aacgagatgt tttacctatt tgcgctggct
    14041 gcctttctgt gtctgatttc tgaaatactg ctaagaaata cggtattgcg aaaaattccc
    14101 tgacaaaatc ttttgcatta aaaatgaaag aaatagatta aaaattaatc agatatttta
    14161 tgtttcgatt tgcacatccg gaatattttt ttctgctgat tgttgttcct gtgttgatag
    14221 ggacttttct ctacacgcag gctttaaaga aacgacgttt gaaaaaattc ggcgacccgg
    14281 aattgttggc tgaattaatg ccaaatgtgt cttatcggcg tcctgttgtc aaattttttc
    14341 tccagctttt tgccgtaatc ctgttgatta tcgttttggc acaaccgcaa ttcgggtcaa
    14401 aagtggaaaa tgttaaacgg cagggcatcg atgtcatgat tgcattggat gtttcaaact
    14461 ccatgatggc gcaggacatt caacccaacc gactcgaaaa agccaaacaa atattgtcga
    14521 aactgattga ccaaatgaat gatgaccgca taggactgat tgtttttgcc ggcgacgctt
    14581 atacccaatt gcccataacg gttgattacg tatcggcaaa aatgtttctt tccgccatat
    14641 cgaccaacat ggtgccaaga cagggaacag ccattggcac ggctattgac atggcgatta
    14701 aatcttttgg acagaaatcg caaagcggaa aagccattat tgttattacg gatggtgaaa
    14761 atcatgaaga cgacgccatc agcgctgcaa aactggctgc ccaaaacggt atcaatgtac
    14821 acgtaattgg cgttggcagc cctgaaggtg cgccaattcc aattcccgga accatgagtt
    14881 tcaaaaaaga caaagatgga aatgtggtcg tctccaaact gaatgaagaa atgtgtaaaa
    14941 atattgctgc agccggcaac gggatatatg tcagagccga taacacaaac aatgcgtatc
    15001 ggattatttc caaacaactc gacacgcttg ccaaatcgac tactgaatca aaagtttttt
    15061 ccgactacag cgaacagtat cagtcatttg ctttgatagc actgatagta ttaatcatag
    15121 actttttcct cttcgacagg aaaaataaaa aattgagcaa aatcaaaatt tttgatattg
    15181 aaaatgaaaa gtaaatttca gaatttttct gtcaaaacaa atgtacattc ggaatttaaa
    15241 aagggagtat ttctcttttt gataatgagt gttctttcgg tttctgtttc agctcaaaag
    15301 gggaacaacg aggtgagagc aggaaacaaa aaatacaaca ataaaaaata catcgaggcc
    15361 gaaatcgatt accgaaaagg acttgaaaaa aatcccaaat cgtttgaagg aaactacaat
    15421 ttgggaaatt cgctttttcg gcaaaaaaaa tatgaagaag cttatcagca atacgaaaag
    15481 gctgcttcgt atgcaccaaa aacagaaaag gaaaaactgg ctgccaccta ccacaacatg
    15541 ggaaattccc tgttgatgca gaagaagatt gaagaaagta ttgaagccta taaacaggct
    15601 ctgagacaca atccaaaaga caacgacacc agatacaatt tagcttatgc ccaatcgttg
    15661 ttgaaaaaac aacagcagca acaaaataaa aacgacaaaa acaaagatca aaaacaagat
    15721 cagaatcagg acaatcagca aaataaacag aacgacaacc aaaaagacca aaaaaatcag
    15781 cagcagcaac aacagcaaca gatgactaaa gaaaatgcgc agcaaatttt ggatgccctg
    15841 tcacaagacg aaaaagaaac tatgaacaaa gccaaaaagt taccatcaaa acaggaacaa
    15901 aaacctgaca aagactggta aattttttgg atttttatct gtatttttgt aataaaaaat
    15961 cttcgaaatt ggaagaatcc gaacagacaa agaaaaattt aaggaattgt aaaaatgaag
    16021 cgcaaaatat ttcttctctt tttaattttt gccggtttca ttggcgtcaa agccgatgaa
    16081 cctgttcgtt ttacggctac ggctccatca accgtaattt tggacaaacc ttttcagttg
    16141 gtctatacta tcaatgcaca aggaaaagat ttgcgcatac ccgaaatcaa ggatttcgat
    16201 attctggcag gtccttttga atcgcgcagt tccagcattc agattgtcaa cggaaaaaga
    16261 agttcttcca tgtcgatttc ctatacttat acgctaatgc ctcaacggac tggaactttt
    16321 accattcctt cggccagcat tatggtaaac aatcaaaaat atacttccaa cggattgact
    16381 ataaaagttt tacctgccga tcagcaatct gatgaatcct cttctcctgc cgataacgga
    16441 aggaaatcgt ccccgaaaaa tatttccaat gaaaatattt ttatccgcac catcccttcc
    16501 aaaacaagtg tttacgaaca ggaggcactt ttaattacct acaaacttta tacgctggta
    16561 gatgtggcgc agtgtgtaaa caaaaaaatg cctgattttg atgggtttct taaacaggaa
    16621 atcgagcaga cacaaaacaa gcaattttct tacgaaaact acaacggacg caactatgga
    16681 acggtagtgc tttatcaaac actcctctac ccgcaacggg caggtaccat tcaaatcgac
    16741 aatgctgttt ttgaatcggt gattcgcgtt caaaatcaaa cttccattca cagtattttc
    16801 gacgattttt ttgattccta taccaatgta acaaaaaatc tcgtagcacc gggactgaaa
    16861 attaccgtca aaccgcttcc tgaagacaaa cctatggttt ttggaggagc agtaggaaat
    16921 ttcaccatga gttcttccat atccgctcaa gaggtgaagg aaaatgaagc tgtaacgctt
    16981 aaaattaaca tcgccggtag tggaaatatg aaactgataa aaacaccgga agttaaattt
    17041 ccgagcgact ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagtggaa
    17161 atatgaaact gataaaaaca ccggaagtta aatttccgag cgactttgaa atttacgacc
    17221 caaaagttac taataatttt aaaacatcgg cttcgggcgt cagtggaacc aaaactatcg
    17281 aatatctgtt tattccgcgt cattctggaa attttgaaat tccaccggta gaattttcct
    17341 atttcgatct ccgtgacaaa acttataaaa tgttgcgcac tccgagttac agcctgaagg
    17401 tattgaaagg ttcgggcaat caaaacgcca ctgttgtaag caactataat ccgaaagaag
    17461 atatccggca aatcaacaaa gacattcgct acatttacac aggcaacatc cgtttgataa
    17521 aaccatcaac tcctgttttc ggcactaccc tggcatggct gctgttcatt attccattgg
    17581 ttgtggttgt tggtttattc ttcttcttcc gcaaaaaagt caaggaaaac gcagatattg
    17641 ctttggtgag aaataaaaaa gccaacaaaa tggctcagaa aaggttgaaa ctggctcaaa
    17701 aattactcaa agaaggcaac aaggatgctt tttatgatga aattttgaaa gccacttgga
    17761 attatttgag caacaaactc tccatacctg ttgcctcact taccagagaa acggtttcgg
    17821 aggaattgaa actaagagga gtaaccgatg aattgacaaa gaagttgatg aaaattttga
    17881 acgattgcga gtttgcacga tatgcaccaa aatccggaca acaggaaatg ggtaatattt
    17941 acgaagaaac tattcaagta atcagcgaat tggaagaagc cataaaaaag taaacaattc
    18001 gcagatagcg aagataagct tgtttaaaaa caccatttta tcttaacaaa taaaatatcg
    18061 gctgtgctgt aacaaaccga ataaaaaatt taaaaataaa ctatgaaacg tctcatatcc
    18121 aatatcatac ttataataac tctcttggct tcgctgcccc tctcagcgca atcagatgca
    18181 gttaaatatg ccaacgacct ctacgccaaa ggaaattttc atgctgctgc agaacagtac
    18241 gaaaaaatac taaaaaacga aggcatagcg cccgagctct actataattt gggaaatgcc
    18301 tattataaaa tgaatgaaat cggattggcc atcctgaatt acgaaagggc tttgaaactt
    18361 tcaccacgct ttgaggatgc ccgattcaat ttgcaactgg cacaacaaaa agtattggac
    18421 aatgtcaatt acaatcagca attttttatt caacgctgga ttaccaattt gatgcacatg
    18481 ttcacttcca accaatggtt tacggcgggt tacctgttct tcttggctgc cttgattttt
    18541 gcttttcttt ttgcatttgg ctcatcggta cgacaacgaa aaaattcttt ctatatcgga
    18601 attgttctgc ttctctttgc tgttaccagc cttatttttt ccggcattcg caaaaacgaa
    18661 gaaaccaatt ctcttcaagc tattattatg acgggagtgg ttacggtgaa aagctcaccc
    18721 gacgaaagtg gaacagattt gtttcagctg cacgaaggaa caaaggttac aataaaaagt
    18781 acattgggta attgggcaga aatcaaaatc ggtaatggaa atgtgggctg ggtagaaagc
    18841 aaaagcatag aaaaaatata aaaaagttgc tggttttcac ggttgcaaat acttctgaaa
    18901 aatgaacctg atagaaacat taagaatttg ggataccaac ttgttcctgg ccttgaatgg
    18961 catacacagc ccattttttg acgggttcat gatggctttc agcaacaaac tggtatggat
    19021 tccgttttat atttccgttt tgtatgtgat gattaaacag tggaaatcgg aagctttctg
    19081 gattgttctt tcttttgccg tttgtatttt actcgccgat caaatttcat cgggaattat
    19141 taaaaatgcc gttcaacgcc tgcgtccttc gcgcgaagaa actattagtg ggctggttca
    19201 tttggtggat ggttatcgtg gtggaaaata tggattcgtt tcttcacatg ctgccaattc
    19261 tcttggtttt gccgttcttt cggcttactt tttccgcaaa agaagctaca cgctgattat
    19321 tttactctgg tcgttgatta cgggctattc acgaatatat ttgggtgtac attatcctct
    19381 cgatgtcatg ggaggatatt tcgtcggatt ttttgtagct attttttgct attgggtact
    19441 aaaaaaactg aaaccgaata ttttttacgt ttcttcccgt tttcgaagca agcgttttgc
    19501 tgattcagcc ttaccgctat tggtgcttac cttcacggtt gtgggaattt ttgtttggag
    19561 tgtgattcgg atattggctt tttaaagaat tgcctgttct aaaaattttg acttcaacac
    19621 ctgcaaatat ctcgattttt ttgattaatt ttgcagccgt taataaaata gtgccttttt
    19681 agctcagctg gtagagcgat tcattcgtaa tgaataggtc tggggttcga gtccccaaaa
    19741 aggctcttta taacgatttc caaaagatga tgtttgacaa tattcttttt gcttcttttg
    19801 ttccacaaat actgatgata ttgggttata tcatgtgttt gtcggcagct acttcatctt
    19861 ctcatgtttc aggcattagt gaaattactc ccataacaca acatcaagta tttttttcga
    19921 ctgaaaacaa gattgaaacc gaaaatactt ttcaatttga agattatttt tttgtttcag
    19981 ccatcattcc atccaaagaa tcaataaagc caatttatta ctttcccaca ataattcctc
    20041 ttgtaaaatg taatgcctgc atttcaaatg gtttcttggc agaacttttt tgtcgtccac
    20101 ctcctactct ctccttttgt taaatcaata aatatttcct gtgtttcggc aattacactt
    20161 ataatttttg ccagaaaccc ttcattaatg gctgtttgtt gcagttaaaa agacgaaaga
    20221 aaatcgaaaa aaattggacg tttttattga attattgagc tttaattcaa tctgaaaaaa
    20281 ctcaataaac gaaaatcggt ttttttaaca atcttgcagc atattgcata caaaaaatgc
    20341 tgaataaata ttcactattg tattcgttta tctatattaa aatcaaaaaa atgaaaaaaa
    20401 tattgatatt actttcttta gttcctacct ttatttttgc ggttgacttg gaaccgaaag
    20461 attcattgaa acatgtttat ctcgaagaaa ttcagattat ttcacacaaa gaaaccttac
    20521 ctgcacaacc ggctgcttct tattcggttg tgtcatccaa tctcatcaat caggcacaag
    20581 tcaattccgt tcgcgacttg agcatactcg ttcccaattt ttttattccc gattacggtt
    20641 cttccatgtc aacctctccc tacattcgtg gtgttggagc gcgcagcacc ggacaatcaa
    20701 tagccttgta tatggacaat gttccatatt ttgaaaaatc aacgttcgat ttcgatttct
    20761 acgacataca gcaggctgaa gttttgcgtg gttcacaaag tactttgtac ggacgcaatg
    20821 ctttgggggg cgtggtaaat atttatacac tttctccctt tgaatatcag ggaacaaaat
    20881 tatcggttac aggtggtaat tacggtttat tgcaagcgca agcttcgcat tatgccaaac
    20941 taagcaacaa aacaggattg tcgctaagcg gttattatca tcatcgcaac ggattcttta
    21001 ccaatacttt taccggaaaa gatgccgatt ggaaaaattc tgccggaggt agagctaaat
    21061 tcgactggca tattacgcct tcgctgcgtg ctcaatacat tatcaattat gattatgtga
    21121 atcaaaatgc attcccttac ggactgtata atgcaactac caagtcgact gcagaaccga
    21181 attttaacga taaaagttcc tatttacgca acatgcttac caatagtgta ctgattgaat
    21241 atcggaacga caaaatacaa ctgacttcta ctacttctta ccagtatttt tcggataaaa
    21301 tgtttatgga tcaggatttt acgcctcttt cctacttttc tctcgaacaa cagcaaaaac
    21361 aacatgcagt aaacgaagaa attgtattga aatcgagaac ctccggcaat tatcagtgga
    21421 gtttcggagc tacaggcttt tatcaaaaat taaataccga tgcgcccgta gctttcaaac
    21481 aagatgcatt gaccaatatt ctgcaaccgg tactggacag ggcacatact gccggagcgc
    21541 cacaaatgac cattaccagt actgtaatgg acattcccgg aacttatgac acaaaaaccg
    21601 ctgaaggggc tttgtatcac caatcgacct tcaatcatct tttaaccgac ggactttcgg
    21661 cgacaatagg tgttcgtgcc gattttgaaa aagtaaattt agactatttt accaatacga
    21721 gactggattt attaatgaaa atgggaaata ggaacattcc aacttctttg gccgatacac
    21781 ttcgaggagc agaatcagtg aaattttccg aatttcttcc aaaagtcagt gtgaaatacg
    21841 catggaacag ccagcagtac atttatgcca acgtttcgaa aggttacaaa gcaggaggtt
    21901 acaacataca gatgtttgcc gacttgatac agaatgattt aatgaattcg ggaaattcgt
    21961 ctgcttctca acccgatgtt cgcaaagcca ttttgtttca accggaatac agttggaatt
    22021 acgaagcagg ctgccaatcg acatttttgg acaacagact gaaaatgcaa ttgtcgttgt
    22081 tttacatgca aatcaccgat atgcaattaa cccgatttgt tcccagcggc aacggacgaa
    22141 tgataaccaa cgcaggcaag gtggacagca aaggaatgga gttctcattg caggcaaatt
    22201 tgggaaaagg ttttgccgcc ggtcttaact atggttatgc acatgcagcc tttaccaatt
    22261 ataccgattc agtaaaaagt aacggaactg taaacgaagt ggattacaaa ggaaaatttg
    22321 ttccttatgc tcctcaaaac accttgaata tcggaggcga atatcttcgc atattcaaaa
    22381 actcgtttat tgatcaattc atggtttcaa tgcaatacaa cgcagccggg aaaatttatt
    22441 gggatgtgga caattccatt tatcaggact tttatggttt aatcaatgca aaagttggtg
    22501 tggagaaagg aaatttcagg gtcgaacttt gggcaaaaaa tctgttcgat acccagtata
    22561 atgcttttta ttttgaatct tttgagaaca agttttttca aaaaggaaat cctgcacaat
    22621 tcggtttgac tttaaaaacc gaattctgaa aaaactttta aaaaataact tttgcttcag
    22681 tatcattgat ccattacgag aaaaacttat tctcgtaatg gattttatat tttcggaaaa
    22741 atctcttcat cgaaaaaaaa taagtaattt cgcataaaat ttatacatac ttcaccctga
    22801 aattatggaa aaaacaacac atacaatgga ggaaaaactt caggaattta aacgtttgct
    22861 ggaaataatg gagcggttga gagccgaatg cccttgggat agagaacaaa ctttcgagag
    22921 catgcgcgtt caaactattg aagaaactta tgaattggcc gatgcaataa tgaaagacga
    22981 caaaaaagcc atcatgacgg aagtaggcga cttgttgctt catgtggttt tttatgcaca
    23041 aatgggaagc gaaaccggcg acttcgacat ttttgatgtt tgtaagaatc tgaacgaaaa
    23101 actgattttt cgtcatcccc acatattttc caataccaaa gccgacaaca gccaaacagt
    23161 cgttcaaaat tgggaagatt tgaaattaaa ggagaaaaac ggcaaccgaa ctgttcttgc
    23221 cggcgttccc gaaactttac cggcattgat aaaagctcat cggattcagg acaaagcgcg
    23281 aaatgttgga tttgactggg aaaaacgcga acaagtatgg gataaggttc aggaagaatt
    23341 tcaggaacta aaagacgaag tagaagcgat ggataaaaat aagatggaag aagaatttgg
    23401 cgacttgctt ttcagcctga tcaatgcagc ccgcctttat gacatcaatc ccgaaaatgc
    23461 actcgaacga accaaccgta aatttattca acgattcaat tacctcgaat ctcaaaccat
    23521 ttcgcaagga aaagacctga gacaaatgtc gctggacgaa atggatgttt tctgggagga
    23581 agccaaaaag aaagagaaac aagaaggtta aaaatcaatt ttattttttt ctttccctgc
    23641 agcaaagcct taataaagta ttagccaaca attatttatc atttgaataa ttgaaagaaa
    23701 tcgaagtttt ttaaaaatgg acattgcaac ctattaaaaa tgtggctttt ggcatgggat
    23761 aaccggcatt tatttcataa gagcagttta aaagattttc acccgttaaa aatagttggg
    23821 tgaatttacc caattggtac gtcgttctgg cattcaacaa ggtaaaattt tcctttttca
    23881 caggattgag agaagaataa agtctatgaa catattgaat tccggaagaa aattttagct
    23941 tttttttcga gtagtcgaat cctgcaaaaa ctttttgttt gggtgctccg gttactgggt
    24001 atttcatatc gagccaactg tagttgacat ttgccgacca aattgaattt atttgataag
    24061 ttatgacgct ttcgaagcca aagtttttaa tttttcccgt atttacgttt attggtcttc
    24121 catttgaaat tactgtctga atcatatttt ttccttcaag ataaaataca gccacatcat
    24181 agaacaaatt tttttcaacc atattttgag aaactgaaaa ttcataattc attacttctt
    24241 cgggcaataa attggggttt tgaggagtaa acataaaaag ttccctgatg gttggatttc
    24301 tgaatccttt gccaactatt gtctttgctt caatattttt ggggagatga aaggataagc
    24361 ccacttgagg aatccattga gaaccggttt tggaatgatt atctaagcga atgcctatat
    24421 ccactgtggc cagcaagccg aagtcttgac gcatatcagc ataacccgct atttcgtaca
    24481 aggtagtatc gataatcgat tttttatttc catcgttgta tttattccat gctttgcctc
    24541 caaatttttg atagtcgaac ccaaaagtaa ttttgttgtt gggcattaaa attgctgatt
    24601 gataaaacgt tataccggat attttatcgt tggagtggaa aagataatcg agtggctgtc
    24661 ctcccatgtt gtatccatca tttataatgt gatttcccca attgtaaaaa aaactcaaag
    24721 ctcctgaagt gtatctgtac ctgttttcta ccgaagcatt tatccatccc cttttaacgt
    24781 gcgaatcgtt gtcaacaatc ggctgatcga tagtgccggg attggaagca ttgaaatgag
    24841 aaatatttgc gttggtggaa acactccagt atctgtccag ccgataaccg agttttgcat
    24901 aaccgcttat ttggtcgaaa tccatatttt cccggtgtcc atcagtccga ttgtatgaaa
    24961 tggcaaattt accggttatc cgatttttat ggaagtgatt gcttgccgaa gattccagcg
    25021 tgctgtaaga gccgtaaccg aagtggaaat tttgttgaac ggtatccttg taaagttttg
    25081 aagtaataat attgataacg ccacccatgg cattggatcc gtaaagagca gaagccgggc
    25141 ctctgaccac ttcaatccga tctgctatta cagattgata caagtctgct ataggatgtc
    25201 ccattaagcc cataaattgt ggttctccat caatcagtat caatactcct gtagtaggat
    25261 ttcctccaat tcctctcatt ttcatcgttc cggcagctcc tgtcgaaatt ccgtagccca
    25321 taattcctcg accggtaata aatagtcccg gaaccagttc attcagtaac ggaattacag
    25381 actggctttg gcgatcaagt atttgtttgt cggtaattac ggataagctc atgggcaaat
    25441 ttcttacatc cgcttcactt ctggtacccg ttacaataac ttcattcata taaatggttt
    25501 tttcttcatt tttcagaata ctgtcccgaa aaagttgtgc tttcagcgaa aatgtcgaaa
    25561 tactgattag aatgaaaatt gcaataaaca tcagtttaca aaagagttgt ttcaaattca
    25621 ttattttatt tcgattttgt ttataagtaa attcaagcaa caagtgcgaa attaaataaa
    25681 atgtttgaaa aactcatttt taggggtatt aaagattaat ttttctcttg gtcttcgatt
    25741 caatttgtat tgactatctt tttatatagt gatccgaaat tgtcgggata taaaaagttg
    25801 tgtggaaaaa ttcttttcag agaatatcca gacctgaaac aaggttatgc tcttacacac
    25861 tccttgagaa tgatttacaa caaaaacagt atcaaagccg ctgc
//
LOCUS       DAOTAX010000026        24943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1639_length_24943_cov_30.783229, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000026 DAOTAX010000000
VERSION     DAOTAX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24943
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1639_length_24943_cov_30.783229"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..2281)
                     /locus_tag="PLM04_04380"
     CDS             complement(137..2281)
                     /locus_tag="PLM04_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HON02348.1"
                     /translation="MKKKILLSTLIIFSIVCTLNAATLLSPNKNLKLEFQLTPSGEPE
                     YSFFYRGKPIILPSKMGFELKNMPSFVNGFQIHKIDTLTFDQTWEPIWGEVRQIRNHY
                     NEMLVCLEQKSTQRTMKIRFRLFDDGLGFRYEFDKQQNLSYFQIMEEFTQFALTADHK
                     AFWIPGDYDTNEYYYTTTKLSEVDASKVDGSGIGTHGYFAKNAVQSPMMMKTDNGLYI
                     NIFEAALVDYPAMQLLIDQKKLVLTSSLVPSAVGVKAFMQTPQNTPWRTIIVSDKATD
                     ILASKMILNLNEPCKIKDTDFIKPQKFIGIWWAMHVPNKWSWNYAHEPNIKLKDTDWN
                     ALKPLPQHGAKTENVKRYVDFAAANGIPSVLVEGWNVGWEDWMGNWKEEVFDFVTPYP
                     DFDVKEIHRYAASKGVKIIMHHETSASVTNYERRLDKAIEFMKENGYDAVKTGYVGRI
                     IPRGEYHDGQWMVNHYIRVAEKMAENKIMLDAHEPVRPTGLHRTYPNWLACEAARGNE
                     FNAWSAGNPPEHETILPFTRLMGGPMDYTPGIFEIKMSYYQPGNPCQVHTTLAKQLAL
                     YVTMYSPLQMAADLPENYEKRMDAFQFIKDVAVDWDDTKYIEAEPGDYITVARKAKNT
                     DDWFVGAITDENARMAVIPFNFLDAKQWYVAIIYGDAANADWKENPMAYKIQKFLVNS
                     KSILKQKLAPGGGAAISLKKATKEEMKGLKKL"
     gene            complement(2402..2911)
                     /locus_tag="PLM04_04385"
     CDS             complement(2402..2911)
                     /locus_tag="PLM04_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON02349.1"
                     /translation="METEYFPIVNEKGEIIGKESREKCHSGSFLLHPVVHLHVLNPNN
                     EIYLQKRAENKDVQPGKWDTSVGGHVDYGETIEEVLKREAFEELGIQKFEPEFLYQYI
                     YTSDVESELVYSYFTFYDGIISPSKDEISEGRFWKDEEIEQLMKKGIFTPNFEYEWKK
                     WKEWNPNHK"
     gene            3266..4303
                     /gene="asnA"
                     /locus_tag="PLM04_04390"
     CDS             3266..4303
                     /gene="asnA"
                     /locus_tag="PLM04_04390"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HON02350.1"
                     /translation="MSYFIRPKNYQPLLDLQQTELGIKKIKDFFQSNLSSELRLRRVT
                     APLFVLKDTGINDDLNGVERAVSFPIKDMNDATAEVVHSLAKWKRVALANYGIQNGYG
                     IYTDMNAIRADEELGNLHSLYVDQWDWERVMSREERNLEFLKEIVRRIYATFLRTEYL
                     VWESFPQIEPCLPSDIVFIHAEELRQMYPELAPKERENEFARIHKAIFVIGIGGKLGD
                     GSKHDGRAPDYDDWSTIAENGLPGLNGDIILWNPVLEMAFEVSSMGIRVDSDALLRQL
                     EMEGKQERLQLYFHKSLMEGRLPLSIGGGIGQSRLCMFFLRKAHIGEIQSSIWPEEIQ
                     QECKKLNIVLM"
     gene            4520..5113
                     /gene="hisIE"
                     /locus_tag="PLM04_04395"
     CDS             4520..5113
                     /gene="hisIE"
                     /locus_tag="PLM04_04395"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HON02351.1"
                     /translation="MNLDFQKMGGLIPAIIQDEWTSKVLMLGFMNEEALEKTLATGKV
                     TFFSRTKNRLWTKGEESGNFLNLVSITPDCDNDTLLVKVHPTGPVCHTGNDTCWGETN
                     QGDFSFINYLQNFIKKRYEEMPEGSYTTSLFKSGVNRMAQKIGEEAVETVIEAVNGTD
                     EGFIYEASDLIYHLIVLLTSKGFSLDDIGKELKKRHK"
     gene            5123..5797
                     /locus_tag="PLM04_04400"
     CDS             5123..5797
                     /locus_tag="PLM04_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON02352.1"
                     /translation="MTKKLLVKYVDVNLYQQEMLVLKDVQLEIYSGEFIYLLGKVGSG
                     KSSLLKSFYGEVPIDSGSAGVLNYDLRNLKSNEIPFLRRKIGIVFQDFQLLTDRTVHD
                     NLEFVLKATGWKDKQAIDEQIEAALLQVGMQNKSYKMPHQLSGGEQQRVVIARALLNS
                     PEMILADEPTGNLDPETGNELVQLLYEICRSSDTTVVMATHNMTWVEKYPGRIIRFEN
                     EQLIEN"
     gene            complement(5802..7283)
                     /gene="proS"
                     /locus_tag="PLM04_04405"
     CDS             complement(5802..7283)
                     /gene="proS"
                     /locus_tag="PLM04_04405"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HON02353.1"
                     /translation="MGKELKELTPRSENYSQWYNDLVIKADLAENSSVRGCMVIKPYG
                     YAIWEKIQSELDRKFKETGHVNAYFPLFIPKSYLSREAEHVEGFAKECAVVTHHRLKA
                     DPEGNGLIVDPEAKLEEELIVRPTSETIIWSTYKNWIQSYRDLPILINQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATEAEALEEAKKMLNVYANFVENFMAIPVVKGVKSPNE
                     RFAGALETFCIEALMQDGKALQAGTSHFLGQNFAKAFDVTFVDKNNKQDYVWATSWGV
                     STRLIGALIMAHSDDNGLVLPPTLAPHQVVIVPIYKQPEQLSAITEKAEKIMKELKKF
                     GITVKYDDDDTKKPGWKFAEYELKGVPVRLAIGARDLENNTVEVARRDTLTKETVSAE
                     NIEIYVSKLLEEIQKNIYQKAFDFRTSVTREVNSYEEFKIEIEKGGFLLCHWDGTPET
                     EEKIKQETKATIRCIPLEGDQTPGVCMVTGKPSKQRVIFARAY"
     gene            complement(7410..7820)
                     /locus_tag="PLM04_04410"
     CDS             complement(7410..7820)
                     /locus_tag="PLM04_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HON02354.1"
                     /translation="MIFQTEITLQAYRKGCHLITREILQHLPVLPEKGLLHLFLKHTS
                     AALTINENADPDVQTDLFEIFSRLVPENQSYYEHTLEGSDDMPAHAKSTLSGVSLLIP
                     ITNGHLNLGIWQGIYLCEFKNRGGNRTLVATIIS"
     gene            8112..9404
                     /gene="tyrS"
                     /locus_tag="PLM04_04415"
     CDS             8112..9404
                     /gene="tyrS"
                     /locus_tag="PLM04_04415"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HON02355.1"
                     /translation="MNFIEELRWRGMIHSMMPGTEEQLAREMTTAYVGIDPTADSLHI
                     GHLVSIMILRHFQRAGHKPIALIGGATGMIGDPSMKSSERNLLDEATLRHNQACIKNQ
                     LTKFLDFGSDAPNAAELVNNYDWMKDYTFLNFIRDIGKHITVNYMMAKDSVKKRLSSD
                     SSEGMSFTEFSYQLIQGYDFLWLYENKNCKLQMGGSDQWGNITTGTELIRRKTGGEAF
                     ALVCPLITKADGGKFGKTESGNVWLDARYTSPYKFYQFWMNVSDADAEKYIKIFTFLS
                     KEEIDALIDEHTKAPHLRILQKKLAQEVTIMVHSEADYQAAVEASNILFGNSTPEALR
                     KLDEDTLLAVFEGVPQFNIPKSSLESGIKAVDLFTETAPVFPSKGEMRKMVQSGGVSI
                     NKNKLKQFDEVINTSYLIDNKYLLVQKGKKNYFLLIAE"
     gene            9719..9789
                     /locus_tag="PLM04_04420"
     tRNA            9719..9789
                     /locus_tag="PLM04_04420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9751..9753,aa:Gln,seq:ctg)
     gene            10030..10731
                     /gene="radC"
                     /locus_tag="PLM04_04425"
     CDS             10030..10731
                     /gene="radC"
                     /locus_tag="PLM04_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HON02356.1"
                     /translation="MEDNDKRLTLREWAEDDRPREKMLRKGIQSLSDAELLAILIRSG
                     SKNETVVELSRRILHECNDNLNELALLSVHDFCQRFKGIGEAKAITVIAALELGKRRK
                     TSEMLERKKITSSKDLLELFEPLLIDLPHEEFWVAFMNSGNKVLETYRLTQGGSKQTV
                     VDVPMLLKMALTKSAHALAVAHNHPSGQNQPSHEDEAITKKIKMGCDAVGITFLDHII
                     VARGKYYSFFDNGKF"
     gene            10798..11718
                     /locus_tag="PLM04_04430"
     CDS             10798..11718
                     /locus_tag="PLM04_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON02357.1"
                     /translation="MPFLEVKNVVKQYAEHLALNNVSLDVPENTIYGLLGPNGAGKTT
                     LIRIINCMTAPDSGTVLLNGKPLTFEDVRHIGYLPEERGLYKKMKVGEQALYFAQLKG
                     MSRHDALKALKYWFQKFEIQSWWNKKVEELSKGMAQKVQFIITILHEPKLLIFDEPFS
                     GFDPVNTDLLKQEILHLKEQGATIIFSTHNMASVEELCENISLIHRSKIVLQGNVAEI
                     RASFSNKTFKVITREPVLDGHYFELVHQTRKNGGVESIIKKVEACNNNILLSEILQQT
                     EILAFEELLPSMNDIFIETVSGHSKNSIEN"
     gene            11755..13068
                     /locus_tag="PLM04_04435"
     CDS             11755..13068
                     /locus_tag="PLM04_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON02358.1"
                     /translation="MSKIHLIIQREYTTRVMKRSFILLTFLTPLFFAAMIILPVWMST
                     LKDEKEKHILVIDQTHQYINALKSNNTYIFDFIDQPLNQVRKTHKKDKKIAAILLITD
                     DLLKNPAAATLYSEEQVNLETKSYIEGLLNEYLREQKLASYNIPDLDKIIQDSNSKIN
                     ISTIRWDEEGKEKQSSAELALIIGMISAFIIYMFILTYGSQVMSGVIQEKTNRVVEII
                     VSSVKPFELMLGKIIGIALVGLTQFFLWIILTFVLLFVAQFFFSNQIDMSMLQQTNQM
                     AAMPAASMENMGNTNILVVLQSFDFVQIGIYFLIYFLGGYLLYASLFAAVGSAVDNET
                     DTQQFSFPLTLPIIFALFVAIYGAQNPDSSLTFWCSLIPFTSPVVMMVRLPYGVAVWE
                     IVLSLAILILSFIATTWVAGKIYRTGILMYGKKTSWKELWKWLNY"
     gene            13146..14048
                     /locus_tag="PLM04_04440"
     CDS             13146..14048
                     /locus_tag="PLM04_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HON02359.1"
                     /translation="MMPPFLKSGDMVQIVSPASAIHPSYVEGAKRTLSEWNLKVLEGK
                     YARSAYGRFAGTKEERIFDLQQALDDPEVKAIFMSRGGYGLVQIIDKINFEQFKKSPK
                     WLIGFSDISILHAAVNRMGIASIHGIMAKHLAELPRTSDAVFHLREILFGRLPVYQLP
                     FHPLNRQGKITAKLLGGNLSVLMGLRGTSFDLPFEDAVLFVEDTGEKPYQVDRMIQNL
                     RIGGIFEKISGLIVGQFTDYESDPLMMQTIEEMFAEAVKNYSYPVCFNFPAGHIDYNL
                     PLLLGENITLEVSAENVLVDFNRQ"
     gene            complement(14158..14586)
                     /locus_tag="PLM04_04445"
     CDS             complement(14158..14586)
                     /locus_tag="PLM04_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02360.1"
                     /translation="MKPTCIAYTNTNEYNIQTCGFSIRPLKNKLYSDEKTILFVLAIF
                     VSCSLMKADPAKKVNVTYQDGKLKVEALHKVRNASNHFIDQVVVKVNGKEMKTIRLDK
                     QTSTASQLVEIELPDLKKGDQIEVVTRCNEFGKKNGKLIL"
     gene            14934..16061
                     /locus_tag="PLM04_04450"
     CDS             14934..16061
                     /locus_tag="PLM04_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HON02361.1"
                     /translation="MAKFWGRNKATFPLTMLLSYVVSFMQAQTETTKPLQLSVSYTGE
                     EAGNFSGGIKRGSTYLGLVSLKAELNTQYAGWWEGGTFMAEGMNTHGGEPAANLTGDF
                     QGVSNIEAGNHTFLNNLWFAQEIGKIKLTAGLQNMDGNFASTEYGSAFINSSFGIPST
                     FAYNIPSPIFPFTALGLRLEWNILENLWLKTAIYDGQPDDFESHPHRLNWEIGQKEGF
                     LSISEIEWGKSLLKNQNGSYKIGFYYHQSTDSLNIKQNNKGAYLIVDQQISKQTAAFA
                     QLAISPKNKNQHYLYAGAGINLKGILRQNSDDLLGLGVAYAALKNFDKKGETAFELTY
                     QYCFNEHFALQPDLQYILHPAGTEEKINNAFAGLLRFYINF"
     gene            16071..18581
                     /gene="feoB"
                     /locus_tag="PLM04_04455"
     CDS             16071..18581
                     /gene="feoB"
                     /locus_tag="PLM04_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HON02362.1"
                     /translation="MQIKTTVLADLEPGQEAVITKIMGHGSFRRRLTEMGFVKGKKIK
                     VIKKAPLQDPIEYEIMGYKVSLRQSEAELVEVMLMDEAQEWLNNQKFEGTFIDNEEKN
                     IPKEEILKHSLLKKEKVIEVALVGNPNSGKTTLFNYASGAHERVGNYAGVTVDSKEAI
                     LKKNGYVFKIVDLPGTYSINEYTPEELFVRNHLTEKMPDVVINVVDASNLERNLFLTT
                     QLIDMDIKVVMALNMFDELEKKGIHFDYRAFGKMLGIPVVPTIASKGKGIDELFKKVI
                     EVYEDADPISRHIHINYGTDIEKAIKEIQDVIWKEPTITDKFSSRYLAIKLLEGDKTT
                     MSQLEKYNNYKEVIKTAEKVRQELEKEYGETAETIITEAKYGFIDGALKETLKLPKKN
                     RRETTHELDDLLTHKIWGYPIFLFFMWLMFEATFKLGNYPMEWIDNGVTLLGNWIENV
                     MPEGALRDLIVQGIIGGVGGVIIFLPNIMILFFFISIMEDTGYMARASFIMDKLMHKI
                     GLHGKSFVPLVMGFGCNVPAIMATRTLKNRKDRILTMIIIPFMSCSARLPVYLLLISA
                     IFYKHQGLILFSLYLIGIMVAVITALLVKKIAFKKEEAPFVMELPPYRIPTLKNTTLH
                     MWDKSKEYLKKIGNVILLVSIIIWALGYFPHNKSISEHYDTQIAKVQQLPLDKEEKET
                     KILTLEVAKESALQEQSYLGQLGHFIQPAMYPLGFDWKMGVSLLAGLSAKEVVVSTMG
                     ILYQAERGADENSASLQEKIQQQVHTSGPLIGQKVFTPITAFAFMIFILIYFPCIAVI
                     SAIKKEGGWQWAVFSVVYNTSFAWLAAFAVYQIGILIV"
     gene            complement(18926..20374)
                     /locus_tag="PLM04_04460"
     CDS             complement(18926..20374)
                     /locus_tag="PLM04_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HON02363.1"
                     /translation="MQEIKFYKGENIPLELHKVRVVQKLHLVPIERRLEALQEGGYNT
                     FRLSTKDVFLDMLTDSGTNAMSDNQMAGMMQADDAYAGSQSFYRLQKAVQDVLGKKYY
                     LPVHQGRAAENIISYAYVKKGSIIPMNYHFTTAMAHITQYGGKIAELLYDEAYVIDSE
                     HPFKGNMNLEKLEEIIQLHGAENIPFIRMEASTNLIGGQPFSIQNLRDVRSIADKYKI
                     RLVLDASLIGENAFLIKQREEEFKNKSLGEILKIMTELADLVYFSARKLSSSRGGGIC
                     TDSNEIYHELEALVPLFEGFLTYGGMSVREMEAMAVGLYETLDETIICQSPNFIKYLV
                     AALDSIGIPVIKPAGVLGVHLDAMQICAHIPQKEYPAGALAAALFLISGIRGMERGSI
                     SNQRDEYGNETYADMELLRLAVPRRVFTLSQIKYVEDRVNWLYDNRQLIGGLRFVYEP
                     PVLRFFMGGLEPVSDWPQKLMAKFKDDFGESL"
     gene            complement(20681..22312)
                     /locus_tag="PLM04_04465"
     CDS             complement(20681..22312)
                     /locus_tag="PLM04_04465"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HON02364.1"
                     /translation="MAKEFKYQELFQLGKDKTEYYLLSKDFVSTAEFEGKEILKVQPE
                     GLTFLARTAFRDCSFLLRPEHQKQVAAILSDPEASENDKYVALTMLRNAEISAKGILP
                     FCQDTGTATIYAKKGSNVFTDGTDEEALSKGVYETYTQENLRYSQNAPLTMYDEVNTG
                     TNLPAQIDIMASHGNEYEFLFIAKGGGSANKSFLFQETKALLQPDTLEKFLTEKLKLL
                     GTSACPPYHIAFVIGGTSADACMKTVKLAAAKYYDELPTTGNELGRAFRDVELEEKIL
                     KAAQNLGIGAQFGGKYFAHDVRIIRLPRHGASCFVGLAVSCSADRNIKAKINKDGIWL
                     EKLEDNPGKYIPEEYRNTGEGNAVKIDLNRPMNEILSTLTKYPVSTRLSLSGTMVVAR
                     DIAHARIKEMLDAGKDLPQYMKDHPVYYAGPAKTPKGMPSGSFGPTTAGRMDTYVDLF
                     QEHGGSMVMIAKGNRSQQVVDACKKHGGFYLGSIGGPAAILAQNSIKKVECIDFPELG
                     MEAVWKIEVEDFPAFILIDDKGNDFFKLIKPTMTC"
     gene            complement(22414..23027)
                     /locus_tag="PLM04_04470"
                     /pseudo
     CDS             complement(22414..23027)
                     /locus_tag="PLM04_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
     gene            complement(23017..23298)
                     /locus_tag="PLM04_04475"
     CDS             complement(23017..23298)
                     /locus_tag="PLM04_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HON02365.1"
                     /translation="MFTVLGCMLAGMVVGFIFRKKHFKIIQSVLFVLIWLLLFLLGAE
                     IGSNPAVIRQTGKLGFDALLIGTAGTLGSILGADLLWKWIKPDKSTHEK"
     gene            complement(23444..24334)
                     /locus_tag="PLM04_04480"
     CDS             complement(23444..24334)
                     /locus_tag="PLM04_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HON02366.1"
                     /translation="MEHHHQHTHTHGESNIKMAFFLNFGFTVFEIFGGIFTNSIAIIS
                     DAIHDMGDSLSLGLAWYFQKKSTKESDKNYTYGYKRFSLLGAIINSIILITGSIFVLY
                     EAIPRLLHPETTNTQGMIVFAVIGILVNGLAVLRLKKGSSINEKVVSLHMLEDVLGWA
                     AILVGSLIMYFFDLPVIDPVLSVLITIYILFHVFRNVKESLHILLQGSPTDVDLEKIS
                     QLLLSIENVDEIHDLHIWSIDGNYNILTVHVVLKQPVTIEKAVEIKNKIRSELLQQGI
                     EHATIEFETTEENCSLQQCC"
BASE COUNT         7676 a         4715 c         4778 g         7774 t
ORIGIN      
        1 gccgaatgag actttgaaaa tattatgtgc aattaattat tagaaattat ataattaaga
       61 acagaacata ttaaacaaat caaaaaaccg gcataattgc ttacaccggt ttttttttaa
      121 attaaaagaa aatagtttat aattttttca atcctttcat ttcttctttt gtggctttct
      181 tcagactaat ggctgcacca ccaccgggag caagtttttg ttttaatatg ctttttgaat
      241 tgaccagaaa cttttgaatt ttataagcca tcggattctc tttccagtcg gcatttgctg
      301 catcaccata aattatagca acataccatt gttttgcatc taaaaagtta aaaggaataa
      361 cggccattcg tgcattttca tccgtaatgg cacccacaaa ccagtcatcg gtgtttttag
      421 cttttcgcgc taccgtgatg taatcgcccg gttcggcttc aatgtatttt gtgtcgtccc
      481 aatcgacagc cacatctttg ataaattgaa aagcatccat tcttttttcg taattttcgg
      541 gtaaatcggc tgccatttgt agtggactgt acattgttac ataaagagcc aattgttttg
      601 ccagtgttgt atgcacttga cagggattac ccggctgata ataactcatt tttatttcaa
      661 aaattccggg tgtataatcc attgggcctc ccattaaacg ggtaaaagga agaatagttt
      721 catgttcggg cggattgccg gcactccaag cattaaattc atttcctctg gctgcctcgc
      781 aggctaacca gttgggatag gttcggtgca atccggttgg tcgtacaggt tcgtgagcat
      841 caagcataat tttgttttct gccatttttt cggctactct gatatagtga ttcaccatcc
      901 attgtccatc gtgatattca ccacgaggaa taattctgcc cacataaccg gtttttactg
      961 catcgtaacc gttttctttc ataaattcta tggctttatc caatcgccgt tcataattgg
     1021 ttaccgaggc cgaagtttcg tgatgcataa taattttaac tcctttggaa gcagcgtatc
     1081 gatgaatttc cttcacatca aaatctggat agggagtaac aaaatcaaaa acttcttctt
     1141 tccagtttcc catccaatct tcccaaccca cattccatcc ttcgaccaac acacttggta
     1201 tgccgttggc tgctgcaaaa tcaacatacc tttttacgtt ttcggttttg gctccgtgtt
     1261 gtggaagggg ttttaaagca ttccaatcgg tgtctttcag ttttatgttt ggctcatgag
     1321 cataattcca cgaccattta ttgggaacat gcatggccca ccaaatccca ataaactttt
     1381 gcggttttat aaaatcggta tctttaattt tacaaggttc attcagattc aaaatcattt
     1441 ttgaagccag tatatcggtt gctttatcgc tgacaataat ggtacgccag ggcgtattct
     1501 gaggagtctg cataaaagct tttactccta cagcactggg aacaagcgaa gaagtcaata
     1561 ccagcttttt ttgatcgata agcagctgca tggccggata atcgaccaat gcagcttcaa
     1621 aaatattgat atacaatccg ttatctgttt tcatcatcat aggactttgc acggcgtttt
     1681 tggcaaaata tccgtgtgtt cctattccag atccatcaac ttttgaagcg tcaacttccg
     1741 aaagttttgt cgtcgtataa taatattcgt tggtatcata gtcgcccggt atccaaaatg
     1801 ccttgtggtc agccgtcaac gcaaattgtg taaattcctc cataatttga aaataactca
     1861 aattctgctg tttgtcgaat tcgtatcgga atcccaatcc atcgtcaaac aaacgaaaac
     1921 gaattttcat ggttcgttga gtggactttt gttcgagaca aaccaacatt tcgttgtaat
     1981 gatttctgat ttgacgaact tctccccaaa ttggttccca tgtttggtca aaagtgaggg
     2041 tgtctatttt atgaatttga aaaccattta cgaaagacgg catatttttt agttcaaaac
     2101 ccatttttga cggtagaatt atcggttttc ccctgtagaa gaaactgtat tcgggttcac
     2161 cggatggcgt caattgaaac tcgagcttca aatttttgtt gggagaaagt agtgtagctg
     2221 catttaatgt gcagacaatc gaaaaaataa tcagtgttga aagcaaaatt ttctttttca
     2281 tgtgttgaat aaatttaaaa aaataattgt atgaataaac aaaattaacg gttttttttg
     2341 aagatttatc cattaggaat taaaatttta caaacatcga ttgcaaacgt ttgaagaaaa
     2401 gttatttgtg atttgggttc cattctttcc actttttcca ttcatattca aaatttggtg
     2461 taaatatgcc tttcttcatc agttgttcaa tttcttcatc tttccaaaat cgtccttcac
     2521 ttatctcatc tttcgatggg gaaataattc cgtcgtaaaa agtgaaataa ctgtaaacta
     2581 attccgactc aacatccgaa gtgtaaatgt attgataaag aaattcaggc tcaaattttt
     2641 ggatgcccaa ttcttcaaag gcttctcttt tcaatacctc ttctattgtt tctccataat
     2701 ccacatgtcc gcctaccgaa gtgtcccatt tcccgggttg cacgtctttg ttttcagctc
     2761 ttttttggag gtaaatttca ttattgggat ttaaaacgtg cagatggaca accggatgca
     2821 ataaaaaaga accgctatga catttttccc tgctttcctt cccgataatt tctccttttt
     2881 cgttcactat gggaaaatat tcagtttcca tgtctcaaaa tctttataag attaaaatag
     2941 ttttcaaagt taatcaaaat tttttaaact ttgttcgtaa aatcgcaaat tgtatatcta
     3001 ttttttatca atactatgct tttactgtac cattgtaaat aaaaatgtcg ataattgata
     3061 tattttgatt ttttaacatc tcaatttggt taaaaatttt attttcaatt tactgatttt
     3121 caatggatta ttatttgttc cgaaaatttt tactcttcct ttccttcttg tgaatcaaaa
     3181 aaatcggtaa ggaatgatta tctttgcttc ttaatttttt aaaacaatag aacattttat
     3241 cgaccatcag caaataaatt aaaatatgag ctattttatt cgaccaaaaa attatcaacc
     3301 tttactcgat ttgcagcaaa ccgaattggg catcaagaaa ataaaagatt tttttcagtc
     3361 gaatctctcc tccgaattgc gtttgcgccg tgtaactgca cctctttttg tcctcaagga
     3421 tacgggaatt aatgacgatt taaacggtgt ggaacgcgct gtaagttttc ccatcaaaga
     3481 tatgaacgat gccactgccg aagttgttca ttcgttggca aaatggaaaa gggtggcgtt
     3541 ggccaattac ggtatccaaa acggatacgg tatttacacc gatatgaatg ccattcgggc
     3601 agacgaagag cttggaaatc tccactcttt atatgtcgat cagtgggact gggaaagagt
     3661 catgagccgc gaagaaagaa atttggaatt tctaaaagag attgtccgtc gtatttatgc
     3721 cacttttttg cggacagaat atctggtttg ggaaagtttt cctcaaattg aaccttgtct
     3781 gccttccgat attgttttta ttcatgcgga agagcttcgg caaatgtacc ccgaacttgc
     3841 tccaaaggaa agagaaaacg agtttgccag aatacacaaa gctatttttg tcattggcat
     3901 agggggcaag ttgggcgatg gaagcaaaca tgatggacgt gctcccgatt atgatgactg
     3961 gtcgacgatt gccgaaaatg gcttgcccgg acttaatggc gatattattc tttggaatcc
     4021 ggttctcgaa atggcttttg aagtttcatc aatgggcatt cgagtggaca gcgacgcatt
     4081 attgcgtcag ttggaaatgg aaggaaagca ggaaagattg caattgtatt tccacaaatc
     4141 actcatggaa gggcgtctgc ctctttccat tggcggaggt atcggacagt cgcgactttg
     4201 tatgtttttc cttcgtaaag cgcatattgg cgaaattcag tcaagtatct ggcccgaaga
     4261 aattcaacag gaatgtaaaa aactaaatat tgttttaatg taatcggttt ccaattattt
     4321 caaccaaata aaattttccc agaataccct ctggtttttg ggattttttg tgccaataaa
     4381 tattgggaag cgaataaatt tttaacgaca aatgaagcga aattcttaca aataatcttt
     4441 tttgccgatt tttctattga atatgggcat ttttttgtac tttcgcttgc tgtaaattca
     4501 aaataaaatt tttgacaata tgaatttaga ttttcaaaaa atgggcggac tgattccggc
     4561 cattattcaa gatgagtgga cttccaaagt cttgatgctg ggatttatga atgaagaagc
     4621 actcgaaaaa actttggcta caggaaaagt tacttttttc agccgaacca aaaatcgttt
     4681 gtggacaaaa ggagaggaaa gcgggaattt tttaaacctt gtttccatta cgcccgattg
     4741 cgacaacgat actttgctcg tcaaagttca tcccaccggc cctgtttgcc atacgggtaa
     4801 tgacacttgt tggggagaaa ccaatcaggg agattttagt tttataaatt atttgcagaa
     4861 ttttatcaag aaaagatacg aagaaatgcc cgaaggttct tatacgactt ctctatttaa
     4921 aagtggtgtg aaccgaatgg ctcaaaaaat tggcgaggaa gctgtcgaaa cagtgattga
     4981 agcagttaac ggaacggatg aaggttttat ttatgaagct tccgatttaa tttatcatct
     5041 tattgttttg ctgacttcaa agggattttc gctcgacgat attggaaaag aattgaaaaa
     5101 acgacacaaa taagtgaaaa tgatgacaaa aaaattattg gtcaaatatg tcgatgtaaa
     5161 tctctatcag caggaaatgt tggtgttgaa ggatgtgcag ctcgaaattt attccggcga
     5221 atttatttat ttgttgggaa aagttggaag tgggaaaagt tctttgctga aatcgtttta
     5281 tggagaagta cctatcgatt caggttcagc aggagtttta aactatgatt taagaaattt
     5341 aaaatctaat gaaattcctt ttttgaggcg aaaaatcggc atcgtttttc aggattttca
     5401 actgctcacc gatcgaaccg tacacgataa ccttgaattt gtattgaagg ctaccggttg
     5461 gaaggacaaa caggccatag atgaacagat tgaagcggct ttgttgcagg ttgggatgca
     5521 gaacaaaagt tacaaaatgc ctcatcaact ttccggcggc gaacagcaac gtgtagttat
     5581 tgctcgggct ttactcaatt cacccgaaat gattttggct gatgaaccga ctggtaatct
     5641 cgatcccgaa accggcaatg aattggttca actgctttac gaaatttgtc gctcgtcaga
     5701 taccacagta gtaatggcca cccacaacat gacttgggta gaaaaatatc ccggacgtat
     5761 tatccgtttc gaaaacgaac aattgataga aaactgattt ttcagtaagc tcgggcaaaa
     5821 atcactcttt gtttggatgg tttgcctgtt accatacaca ctcccggagt ttggtctcct
     5881 tccagaggaa tacaccgaat ggtagctttg gtttcttgct tgattttttc ttcggtttcc
     5941 ggtgtaccat cccaatgaca aagtaaaaat ccaccttttt ctatttcaat cttaaattct
     6001 tcgtacgaat ttacttctct ggtaaccgaa gttctgaaat caaaagcttt ttgataaata
     6061 tttttctgaa tttcctccag aagtttggaa acatatatct caatattttc ggccgaaaca
     6121 gtttcctttg ttaaagtatc ccgacgtgcc acttcaaccg tattgttttc caaatcacgt
     6181 gcgccgatag ccaatcttac tggaacgcct ttcagttcgt attcggcaaa tttccaaccc
     6241 ggttttttgg tatcgtcatc atcatatttc actgtaattc cgaacttttt caactccttc
     6301 ataatttttt ctgccttttc ggtaattgca gatagctgtt cgggttgttt gtaaatagga
     6361 acaatgacta cttgatgtgg tgccagcgtt ggtggcaaaa ccagcccgtt gtcgtcggag
     6421 tgagccatga taagcgctcc gataagtctg gttgaaactc cccaagaagt tgcccataca
     6481 taatcctgct tgttgttttt atcgacaaat gtaacgtcaa atgctttcgc aaaattttgt
     6541 cccagaaagt gggaagtacc tgcttgaaga gcttttccat cttgcatgag tgcctcaata
     6601 caaaatgttt ccaaagctcc ggcaaaacgt tcattgggcg atttcacgcc tttcaccact
     6661 ggaattgcca tgaaattttc aacaaagttg gcataaacat tcaacatttt ttttgcttct
     6721 tccagtgctt ctgcttccgt tgcatgagct gtatgacctt cttgccacaa aaactcagcg
     6781 gtacgcaaaa acaatcttgt gcgcatttcc cagcgtacca catttgccca ttgattgatc
     6841 aatatcggca aatcacggta cgattgaatc cagtttttgt aagtactcca aattatggtt
     6901 tcggaagtgg gtcttacaat cagttcttct tccagcttag cttcaggatc cacaatcagt
     6961 ccattccctt caggatcagc tttcagacga tggtgcgtaa ccactgcaca ttctttggca
     7021 aaaccttcca cgtgttcggc ttcccgactc aaataggatt tgggaataaa cagcggaaaa
     7081 taagcattga catgccctgt ttctttgaat tttctgtcga gttccgattg tatcttttcc
     7141 caaatagcat agccgtaggg tttaataacc atacaacctc ttactgaaga attttctgcc
     7201 aaatcggctt ttatcaccaa atcattgtac cactgtgagt agttttccga acggggtgtc
     7261 aattctttaa gttcttttcc cataatttgc gtataatgat atttcttttt gaaaaggaaa
     7321 cgatctgtaa tttgtaggat tgtttcccga aaaatttcgg agtgcaaagt taatcaaaat
     7381 ttacgactta tttgcttgct gaatcaggtt taactgatta tggtggctac caaggtacga
     7441 tttccgcctc tgtttttgaa ttcgcaaaga taaattccct gccatattcc aagattcaaa
     7501 tgtccattgg taatggggat aagcagactt actccggaaa gggtgctttt ggcatgtgca
     7561 ggcatatcat cgctgccttc cagtgtatgt tcgtagtaag attgattttc cggcaccagt
     7621 cggctaaaaa tttcaaacag atccgtttgt acatcaggat cggcattttc gtttatggtt
     7681 aaggcggcag aagtatgttt cagaaaaaga tgcagcaatc ctttttcggg caggacaggc
     7741 aaatgttgta aaatttcgcg tgtaattaaa tggcagccct ttcggtaagc ttgaagtgtt
     7801 atctcagttt gaaatatcat tgtttgaata tttttcgaca aaaataactc ttcttaaaaa
     7861 tttgtgcaat aaaccgacaa agagaaaatt tgattacttg atttcgtcaa cacatttttt
     7921 tacaaaaggg aagcggttgt agtcgttgcg cattaaattg tcgttccaga aaatcaaatc
     7981 aatatcaata tcaataactc aaagtaagat tttattttac cattgttttt tttgagtttt
     8041 cataggtctt tgggtaaatt gaaaataaac ttgtaaattt gcactccgaa ttaaaaaacc
     8101 tgaatttgca catgaatttt attgaagaat tacgttggcg gggaatgatt cattccatga
     8161 tgcccggcac ggaagaacaa cttgccagag aaatgactac agcttatgtg ggtatcgatc
     8221 caacagccga ttcgctccat attggtcatt tggtcagtat tatgattttg cgtcattttc
     8281 aacgtgcagg ccataaaccc atcgctctga taggtggcgc tacaggaatg attggagatc
     8341 cttccatgaa atcatccgaa cgaaatctgt tggacgaagc taccttgcga cacaatcagg
     8401 cgtgcattaa gaatcagttg acaaaatttc tcgatttcgg cagcgatgcg cccaatgccg
     8461 ctgaattggt caacaattac gattggatga aagattatac ctttcttaat tttattcgcg
     8521 atattggcaa gcacatcacc gttaattaca tgatggcgaa agattccgta aaaaaacgtt
     8581 taagcagcga ttcgagcgaa ggaatgtctt ttaccgaatt ttcctatcag ttgattcagg
     8641 gttacgattt tttgtggctt tacgaaaata aaaattgtaa acttcagatg ggaggttccg
     8701 atcaatgggg aaacattact accggaaccg aactgattcg ccgaaaaact ggtggcgaag
     8761 ccttcgcttt ggtttgcccg ctgattacca aagccgatgg tggcaaattt ggtaaaacgg
     8821 aaagcggaaa tgtttggctt gatgctcgtt atacttctcc ttacaaattt tatcagtttt
     8881 ggatgaatgt aagcgatgcc gatgccgaga aatatattaa aatatttact tttctgagta
     8941 aggaagaaat cgatgcactg atcgacgaac ataccaaagc gcctcatttg cgaattttac
     9001 aaaaaaaact ggcgcaagaa gttaccatca tggtacattc tgaggctgat tatcaggctg
     9061 ccgttgaagc ttccaatatt ttatttggaa attctacgcc tgaagctttg agaaaactcg
     9121 acgaggatac attgctggcc gtttttgaag gagttccgca attcaacatt ccgaaatctt
     9181 ctctcgaaag cggaataaag gcggtcgatc tttttacgga aaccgcaccc gtttttcctt
     9241 caaagggaga aatgagaaaa atggtacaat cgggaggagt aagcataaat aaaaataaac
     9301 tgaaacagtt tgacgaagtt atcaacactt cttaccttat cgacaataaa tatttactgg
     9361 ttcagaaagg gaagaaaaat tattttctgc tgatagccga atagaaaatc gcactacttc
     9421 aacatatttt ggctgtaaaa ttttgacaga aaaaaaatcg gttgtatctt tgtgcccgct
     9481 tgctgaaaga ttgcagcgaa agtttgtttg tctcatggtg taatggtagc actgcagttt
     9541 ttggtactgc ctgtctaggt tcgaatccta gtgagacaac tgtttttctg gcggttgagc
     9601 tttttcaaaa ggttcaatcg ttttttttac aagccaaaaa aactctttgc cacaaacaaa
     9661 ataatttgta tttttgcaaa gggttttaaa gttcccgatt tctctgaaat acacattctg
     9721 tctcatggtg taatggtagc acgtcagatt ctggatctgc ctgtctaggt tcgaatccta
     9781 gtgagacaac tacttcccaa cctgatttct cctttttttc tgtatcgatt cacaagataa
     9841 tctttccgta ttgtttgcaa gtttactaaa ctgatggtat ttttatgccc gaaaaaattt
     9901 atgaaccttt ttattgttca aaacaattta ttccgacaat aaaagtattt tcgtaaatcc
     9961 ataaccaatc gtcctgcttt aattttttat tccttttttc atagatttgt ttgcaaaaca
    10021 ttttataaaa tggaagacaa tgacaagcgt ttgacacttc gggaatgggc agaagatgat
    10081 cgcccacgtg aaaaaatgct gcggaaaggt attcaaagtc tttccgatgc cgaattgctg
    10141 gctattctca ttcgctcggg cagcaaaaat gaaaccgtcg ttgaactttc tcgccgtatt
    10201 ttgcatgaat gtaacgataa tttgaatgaa ttggcgctgt tgagtgttca tgatttttgt
    10261 cagcgtttta agggaattgg cgaagcaaaa gccattacgg tcattgctgc tcttgaactg
    10321 ggcaaacgcc gaaaaaccag tgaaatgttg gaacgtaaga aaattacttc cagcaaggat
    10381 ttactggaac tttttgaacc tttactgatt gatttgccac acgaagaatt ttgggttgca
    10441 tttatgaaca gtgggaacaa agttttggaa acttatcgtc tgacgcaagg tggcagcaag
    10501 caaacagttg tggatgttcc catgttgctg aaaatggcac ttacaaaatc ggctcatgct
    10561 cttgctgtgg cacacaatca tccttcggga caaaatcaac ccagccatga agacgaagct
    10621 attaccaaaa aaatcaagat gggatgcgat gcggttggaa ttactttttt agatcacatc
    10681 atcgttgctc gtggaaaata ctacagtttt tttgataatg gaaaattcta agtcattttg
    10741 ccgaaaaatg aaaattattt cttctaaaaa tctataaaaa caattttgta aagaattatg
    10801 ccttttttgg aagttaaaaa tgttgtcaaa caatatgcag agcatctggc tttaaataat
    10861 gtgagtctgg acgttcccga aaataccatt tatggcttac ttggcccgaa cggtgcagga
    10921 aaaaccacac tgatacgtat cattaactgc atgactgctc ccgatagtgg aacagtgctt
    10981 ttgaacggaa aacctctgac gtttgaagat gtgcgtcata ttggatattt gcctgaagaa
    11041 cgcggacttt acaagaaaat gaaagttggt gaacaggcgc tttattttgc ccaactgaaa
    11101 ggaatgtcgc gacacgatgc ccttaaagct ttaaaatact ggtttcaaaa atttgaaatt
    11161 caatcgtggt ggaacaaaaa agtggaagaa ctttccaaag gaatggcaca gaaagttcaa
    11221 ttcattatca ccattcttca cgaacccaaa ttgctgattt ttgacgaacc attcagcgga
    11281 ttcgatccgg tgaacaccga tttgctgaaa caggaaattc ttcacttgaa agaacaggga
    11341 gccaccatta ttttttctac gcacaatatg gcttcagtgg aagaactttg tgaaaatatt
    11401 tcactcattc atcggtcgaa aattgttttg cagggaaatg ttgccgaaat tcgggcttcg
    11461 ttttctaaca aaactttcaa agtgataacc agagaacccg ttttggatgg tcattatttt
    11521 gaattggtac atcaaactcg gaaaaacggt ggggtagaaa gtatcataaa aaaagtggag
    11581 gcttgcaata acaacatcct tttgtccgaa attttacaac aaaccgaaat tttggctttt
    11641 gaagaattat tgcccagcat gaacgatatt tttatagaaa ccgtcagtgg gcactcgaaa
    11701 aattcaattg agaattagtg tcataataac tttcaaatca aacaaatttt cagcatgtct
    11761 aagattcatc tcattattca aagagaatat actacccggg tgatgaaacg ttcatttatt
    11821 ttgttgacgt ttttaactcc cttgtttttt gctgccatga ttattctgcc tgtttggatg
    11881 tccaccttga aggatgaaaa ggaaaaacat attttggtta ttgatcaaac ccatcaatac
    11941 atcaatgcct tgaaaagtaa caatacatat atttttgatt tcatcgatca accactcaat
    12001 caagtgcgta agacacataa aaaggacaaa aaaattgctg ccattttact gattaccgat
    12061 gatttgttga aaaatccggc tgccgcaaca ctttactccg aagaacaagt gaatctggaa
    12121 acaaaaagtt acattgaagg tttgctgaat gaatatcttc gcgaacagaa actggcttcg
    12181 tacaatattc cggatttgga taaaataatt caggattcca attctaagat aaacatttcg
    12241 accattcgtt gggatgagga agggaaggaa aaacaaagtt cggccgaatt ggcgctgatt
    12301 atcggaatga tttctgcctt catcatttac atgtttattc ttacctacgg ttcgcaagtg
    12361 atgagcggcg ttattcagga gaaaaccaat cgtgtggtag aaattattgt ttcttcggtc
    12421 aaaccttttg aactcatgtt gggaaaaatt attggcattg cattagttgg cctcacacaa
    12481 ttttttcttt ggattattct tactttcgtt ttgctgtttg tagctcaatt tttcttttcc
    12541 aatcaaatcg acatgagtat gcttcagcag acaaatcaga tggctgccat gcctgctgct
    12601 tcaatggaaa atatgggtaa cacaaatata ttggtagttc tccaaagttt cgatttcgta
    12661 caaatcggca tatattttct tatctatttt ctgggtggct atttgcttta tgcttcgctt
    12721 ttcgctgctg ttggttcggc agtggacaac gaaaccgata cgcaacaatt tagtttcccg
    12781 ttgactttgc ctattatttt tgctttgttt gtagccattt atggagctca aaatcctgat
    12841 tcctccttga cgttctggtg ttcgctgatt ccctttactt cgccggtagt tatgatggtt
    12901 cgtttgcctt atggcgtggc cgtgtgggaa attgttcttt cgctggcaat tctgattctt
    12961 tccttcatcg ctaccacttg ggtggcaggg aaaatctaca gaacaggaat tttaatgtat
    13021 ggtaaaaaaa cttcatggaa agaactttgg aaatggttga actattgatt attcatacgt
    13081 cagattcctg tggtttaatt agtttgtgat ggttcaatta ttgataaaaa atctcattat
    13141 tgcttatgat gcctcctttt ttgaaatcgg gagatatggt tcaaattgtt tctccggcaa
    13201 gcgctatcca tccttcctac gttgaaggag caaaaagaac tttaagtgaa tggaatttga
    13261 aagtcctcga ggggaaatat gcacgttctg cctatggtcg ctttgccgga acaaaagagg
    13321 aaagaatctt tgatttgcaa caggcgcttg atgatccgga ggtaaaagcc attttcatga
    13381 gtcgcggtgg ttatgggctg gtgcaaatta tcgacaaaat caattttgag caatttaaaa
    13441 aatcacctaa atggttgatc ggattcagcg atatttccat cttgcatgca gcagtaaaca
    13501 ggatgggtat tgcttccatt cacggtataa tggccaaaca tcttgccgaa ttgcccagaa
    13561 cctctgatgc agtgtttcat ttgagagaaa ttttgttcgg gagattgcct gtttatcaac
    13621 ttccttttca tccactcaat cgtcagggaa aaattaccgc caaactgctg ggtggaaatt
    13681 tgtcggtttt aatgggattg cgcggtactt ctttcgattt gccctttgag gatgccgttt
    13741 tgtttgttga agatacagga gaaaagcctt accaagtcga cagaatgatt caaaacttac
    13801 gcataggtgg aatttttgaa aaaatttccg gtctgattgt tggccaattt accgattatg
    13861 agtccgatcc attgatgatg caaaccatcg aagaaatgtt tgccgaagct gtaaaaaact
    13921 attcttatcc ggtttgtttt aattttcctg ccggccacat cgattacaat ttaccgcttt
    13981 tattgggtga aaacataact ttggaagtgt ctgcagaaaa tgtgctggtc gatttcaata
    14041 gacaataaaa accttgtgtt tcggtagaat cggataaagc aacactcaac aaattgttga
    14101 acagaatcta ttcaaatttg ttgagtgccc atttaacacc aaaacacata caaaatgtca
    14161 taaaataagt ttaccgtttt ttttaccaaa ttcgttgcag cgggttacaa cttcaatttg
    14221 atcccctttt tttaggtcag gaagttcgat ttctactaat tgagatgctg ttgaggtttg
    14281 tttgtccaac cggatcgttt tcatttcttt tccgtttact ttcacaacca cctgatcaat
    14341 aaaatgattg ctggcatttc taactttatg caaagcttct acttttagct ttccatcttg
    14401 ataagttaca ttgacttttt ttgccggatc ggccttcatc aatgaacagg aaacaaatat
    14461 tgccaataca aataaaattg ttttttcatc agaatataat ttatttttta aaggtctgat
    14521 ggagaaccca catgtctgaa tattatattc attcgtgttt gtgtatgcaa tacatgtggg
    14581 tttcatatct ccatcaattt ttataattta taaaatgttt ggttttgtaa gtgctgaaaa
    14641 atctccaacc tacttaaaga aataatttat gcgagttagc ctgcgagtca gcagttgaaa
    14701 ctgattctca taaattattc aaatcaatat caagttgtct aaaaatggtt aatgaattga
    14761 tatttgaatg attattagga aatttcaaat ttattgaaat ttttttggat attattattt
    14821 agaatcaatc taaattgcgt aactttgcgt ctccaaaaca agagtagaaa cagaaagaaa
    14881 ttttattaag attaatggga aaaaattcag accaaaatcg gatacaatgg ttaatggcaa
    14941 aattttgggg tagaaataag gcaactttcc cgctgacaat gctgctgagt tatgtcgttt
    15001 catttatgca ggctcaaacc gagacgacaa aacctttgca gttatcggtt tcatatacgg
    15061 gagaagaagc aggaaatttc agtggaggaa taaagcgtgg cagcacgtat ctcggactgg
    15121 tatcgctgaa agctgaattg aatacacagt acgctggttg gtgggaaggc ggaaccttta
    15181 tggcagaagg aatgaatacg catggcggag agccagcagc aaatttgacc ggcgactttc
    15241 agggcgtttc gaatattgaa gccgggaatc ataccttttt gaacaatctt tggtttgctc
    15301 aggaaatagg aaaaattaaa ctcactgccg gactgcaaaa tatggatgga aactttgcct
    15361 ccaccgaata tggcagtgct ttcatcaaca gttcttttgg cattccttct acttttgctt
    15421 acaatattcc aagtcccatt ttcccattta cagcactcgg actccgcttg gaatggaata
    15481 ttttggaaaa tttatggttg aaaacagcca tttacgatgg ccaacctgat gatttcgaat
    15541 cccatcctca ccgtttgaat tgggaaatag gtcaaaaaga aggattcctt tctatcagtg
    15601 aaatagaatg gggaaaaagt ctgctgaaaa atcaaaatgg ttcttacaaa attggatttt
    15661 attatcatca gtcaacagac agtttaaaca taaaacaaaa caacaaaggc gcatatctga
    15721 ttgtcgatca gcaaatcagt aagcaaactg cagcttttgc tcaattggca atcagcccaa
    15781 aaaataaaaa tcaacattac ctgtatgccg gagccggaat caacctgaaa ggaattttaa
    15841 gacaaaattc cgacgattta ttgggattag gagttgctta tgcagcattg aaaaatttcg
    15901 acaaaaaggg agaaacagct tttgaactca cttatcagta ctgtttcaac gaacatttcg
    15961 ctctccagcc cgacttgcaa tatatacttc atccggcagg cacggaagaa aaaataaata
    16021 atgcttttgc tggattgctg cgtttttata ttaattttta aacactcgtc atgcaaataa
    16081 aaacaaccgt attggctgat ttggaacccg gtcaggaagc cgtgatcaca aaaataatgg
    16141 gacatggatc attccgaaga cgactgacag aaatgggttt tgtcaaaggg aaaaaaataa
    16201 aggttatcaa aaaagcaccc ttgcaggatc ccattgagta cgaaataatg ggttacaagg
    16261 tttctttgcg tcaatcggaa gccgaattgg ttgaagtaat gctgatggac gaagcgcaag
    16321 aatggttgaa caatcaaaaa tttgaaggga ctttcattga taacgaagaa aaaaatattc
    16381 ctaaagaaga aattctcaaa cattcgctac ttaaaaagga aaaagtgatt gaagtggctt
    16441 tggtagggaa tccaaattca ggaaaaacga cacttttcaa ttacgcatcc ggagcacacg
    16501 aacgagtggg aaattatgcc ggagtaactg tagattcaaa agaagccata ctgaaaaaaa
    16561 acggatatgt ttttaaaatt gttgatctgc ctgggactta ttccatcaac gaatatacgc
    16621 ctgaagaact tttcgtaaga aatcatctca ccgaaaagat gcccgacgtc gtgatcaacg
    16681 ttgtggatgc ttctaatctg gaaagaaatc tttttctcac tacccaactt atcgacatgg
    16741 acataaaagt ggtaatggcg ctcaacatgt ttgacgaact ggagaaaaaa ggaatacatt
    16801 tcgactaccg cgcattcggg aaaatgctgg gaattcctgt ggtgccaacc atcgcatcca
    16861 aaggaaaagg cattgatgaa ttatttaaaa aggtaataga agtttacgaa gatgctgatc
    16921 ccatttcccg acacattcac atcaattatg gtacagacat tgaaaaagct atcaaggaaa
    16981 ttcaggatgt aatttggaaa gagcccacca taaccgacaa attctcttca cgatacttgg
    17041 ctatcaaatt actggagggt gataaaacca ctatgtccca actcgaaaaa tacaacaatt
    17101 acaaggaagt aataaaaact gcagaaaaag taagacaaga acttgaaaaa gaatacggag
    17161 aaacggcaga aaccataatt actgaagcaa aatacggatt tattgacgga gcgttgaaag
    17221 aaacgctcaa acttcccaaa aaaaatcgac gagaaactac gcacgaactg gatgacctgc
    17281 tgactcacaa aatatggggc tatcccattt ttctattctt catgtggttg atgtttgaag
    17341 ccactttcaa actgggaaac tatccaatgg aatggatcga caacggggtc acgctgctgg
    17401 gaaactggat agaaaatgtt atgcccgaag gagccttgcg ggatttgatt gttcagggta
    17461 tcattggtgg tgtgggtggt gtaatcatat ttcttcccaa cattatgatt ttgtttttct
    17521 ttatatcgat tatggaggac accggttata tggccagagc atcttttatt atggataaac
    17581 tcatgcacaa aatcgggtta cacgggaaat cgtttgttcc gctggtaatg ggttttggct
    17641 gcaatgtccc ggccattatg gctacccgaa cgctgaaaaa cagaaaagac cgaattttaa
    17701 ccatgattat tattcctttc atgtcgtgca gcgcccgact accggtttat ctgctgctca
    17761 tttcggctat tttttacaaa catcaaggct tgatcctgtt ttcgctttac ttgattggaa
    17821 tcatggtagc cgtcattaca gcattgttgg taaagaaaat tgcttttaaa aaagaagaag
    17881 cgccttttgt gatggaattg cctccttacc gcatacccac attaaaaaac accaccctgc
    17941 acatgtggga caaaagtaaa gaatacctga aaaaaatagg taacgtcatt ttacttgttt
    18001 ccatcattat ttgggcgttg ggatattttc ctcacaacaa atcgatcagc gaacattacg
    18061 atacacaaat tgcaaaagtt caacaacttc ctcttgataa agaagaaaaa gaaacaaaaa
    18121 ttttgacttt ggaagtagca aaagaatcag ccttgcagga acaatcttat ctcggtcagt
    18181 tggggcattt tattcagccg gccatgtatc cgctgggttt cgactggaaa atgggcgtca
    18241 gtttactggc cggattatca gcaaaagagg tagtggtgag cacaatggga attttgtatc
    18301 aagccgaaag aggagccgat gaaaattctg cttcgcttca ggaaaagatt caacaacagg
    18361 tgcatacttc aggaccgttg ataggtcaaa aagtttttac gcccataacg gcatttgcct
    18421 ttatgatttt tatcctgata tatttcccct gtattgctgt catatcggct attaaaaaag
    18481 aaggtggctg gcaatgggca gtattctccg ttgtgtacaa tacttctttt gcctggctgg
    18541 cggcttttgc agtttaccaa attggaatcc tgattgtatg atattaataa aaacgtaatt
    18601 ttataattta ttcatgccat gacacaaacc attataacct acctaattat tgtaggagcg
    18661 atagtaattt cggccttaaa aatagtaaaa acaattcgca gcaaaaagaa ttctccctgt
    18721 agtggatgca acggctgcga aataaaacaa gaaattctga aaaacagtaa agataatctt
    18781 ttccatgccc ccgataaatg ttgttccggg gaaaaagaaa atttttcgtt caacaaaaca
    18841 agttttttaa caaaaaatca attagttgac caaaaacatc cgtaaaaata agagagagaa
    18901 aaatttccct ccctcatttt tcaaattata aactctcgcc aaaatcatcc ttaaattttg
    18961 ccataagttt ttgtggccaa tcgcttaccg gttcaagtcc tcccataaag aagcgcagaa
    19021 caggtggttc ataaacaaat cgaagtcctc cgataagttg tcgattatcg tacaaccaat
    19081 ttactcggtc ttcaacatat ttaatttgtg aaagtgtaaa aacacggcgt ggaacggcta
    19141 aacgcagcaa ttccatgtcg gcgtaagtct cattaccgta ttcatcacgt tggttcgaaa
    19201 tagaaccgcg ttccattcct cgaataccgg aaatcagaaa gagggctgca gcaagtgcac
    19261 ctgcaggata ttccttttgt ggaatgtgag cgcatatctg catggcatcc aaatgaacac
    19321 caagcacacc tgccggtttt atgacaggaa tgcctatgga gtcgagtgcg gcgacaagat
    19381 attttataaa atttggactt tggcagataa ttgtttcatc caatgtttca tatagtccaa
    19441 cggccatcgc ttccatttcg cgtaccgaca tacctccata agtaagaaat ccttcaaaaa
    19501 gtggaaccag agcttccaat tcgtgataaa tttcattact gtcggtacaa attccaccac
    19561 cccgcgatga actgagtttt cgcgccgaaa aataaaccaa gtcagctaat tcggtcataa
    19621 ttttcagaat ttcacccaat gatttatttt taaactcttc ttcccgttgt ttaatcaaaa
    19681 aagcattttc tccaatcaag ctggcatcga gtaccagacg gattttatat ttatcggcta
    19741 ttgaccttac atcacgtaaa ttttgtatag agaatggttg tccgccaatc agatttgtag
    19801 aagcttccat acgaataaac ggtatatttt cagctccgtg cagttgaatg atttcttcca
    19861 gcttttcgag attcatattt cctttgaaag gatgctcaga atcaattaca taggcttcat
    19921 catagagtag ttcggcgatt tttccgccat attgcgtgat gtgggccata gctgttgtaa
    19981 aatggtagtt catcggtatt atgcttcctt tttttacata ggcataagaa ataatatttt
    20041 ctgcagcacg accctgatga acaggcaaat aatatttttt acccaaaaca tcctgaacag
    20101 ctttttgaag tctgtaaaaa ctttgtgatc cggcataagc atcatcggcc tgcatcatgc
    20161 cagccatttg attatcactc atagcattgg tgccgctatc ggtcaacata tccagaaaaa
    20221 cgtctttagt actcaatctg aatgtattgt atccaccttc ctgcaatgct tccaatcgcc
    20281 tttcaatggg gacaagatgt aatttctgta ctactctaac tttgtgtaat tcaaggggaa
    20341 tgttttcgcc tttgtaaaat ttgatttctt gcataataaa aaaataaaaa gttaatacta
    20401 cattttgaag ttattataat gatgtaaaat tttttaatta aagtagttat cgggatatta
    20461 tttctttcgt tggatgaaaa tatttttttc taaataaacc atcgaataca atattcatta
    20521 caaaagtaat taattaattg gtatttattg agtaaaattg tacttattct ttagtagagt
    20581 tttgattaaa ttatagtaat tttttgtaaa tattgaaggg cagctttttc gaactgccct
    20641 tcaataaaag gaaaaatgac attaaaaaaa tcgaagctga tcaacaagtc atggttggct
    20701 taatcagttt gaagaagtcg tttcccttgt cgtcgatcag aataaaggcg ggaaaatctt
    20761 ctacctcgat tttccaaaca gcttccattc caagttccgg gaaatcgata cattcaactt
    20821 tttttatgga attttgagcg agaatggcag ccgggccacc aatagaaccc aaataaaaac
    20881 cgccatgttt tttacaggca tctaccactt gctgactgcg gttgcctttg gctatcatca
    20941 ccatactgcc gccatgttcc tgaaacaaat ctacataagt atccattcta ccggctgtag
    21001 tagggccaaa cgaaccggaa ggcattcctt tgggcgtttt ggcggggccg gcatagtaca
    21061 ccggatgatc tttcatatat tgtggcaaat ctttccctgc atcgagcatt tcctttatgc
    21121 gggcatgagc aatatctctg gccactacca ttgttcccga aagagaaagt cgtgtggaaa
    21181 ccggatattt tgtcaatgtg gacaaaattt cattcatcgg acgattcaaa tcgattttca
    21241 ctgcgtttcc ttcacctgta ttgcgatatt cttcaggaat atatttaccg ggattatcct
    21301 ccagtttttc cagccagata ccatctttat ttatctttgc cttaatgttg cgatcggcag
    21361 aacaagatac cgccaatcct acaaagcagg aagctccatg acgcgggagt cggataattc
    21421 gcacatcgtg agcaaaatat tttcctccaa attgagcccc aatacccaaa ttttgagctg
    21481 ctttcaggat tttttcttcc agttccacgt ccctgaaagc tcttcccaac tcatttcctg
    21541 tagttggcag ttcgtcgtaa tacttggctg cagcgagttt aaccgttttc atgcaagcat
    21601 cggcagatgt gccgccgata acaaatgcaa tatgataggg tggacaagcc gacgtgccca
    21661 ataatttcaa tttttcggta agaaattttt ccagcgtgtc gggttgcagc aaagctttcg
    21721 tttcctgaaa cagaaatgat ttgttggccg aaccgccccc ttttgcgata aaaagaaatt
    21781 cgtattcgtt accatgactg gccataatat caatctgggc gggaagattg gtaccggtat
    21841 tcacttcgtc gtacatggta agtggagcat tttgcgaata acgcagattt tcctgcgtat
    21901 aagtttcata aactcctttg gataaagctt cttcatccgt tccatcagtg aaaacgttac
    21961 tgcctttctt ggcataaatt gttgccgtac cggtatcctg gcagaaagga agaatacctt
    22021 ttgcagaaat ctctgcattg cgaagcatgg ttaatgctac gtatttatcg ttttcgctgg
    22081 cttcggggtc ggaaaggatg gccgcaacct gtttttgatg ttcgggacga agcagaaaag
    22141 aacagtcgcg aaaagcagta cgggcaagaa aagtcaaacc ttccggctga acttttaaaa
    22201 tttccttgcc ttcaaactct gccgtcgaaa caaaatcctt cgatagtaaa taatactcgg
    22261 ttttgtcttt tcctaactga aaaagttcct gatatttgaa ttcttttgcc ataaacgtta
    22321 taattttttt gaatgagcga tagattgaaa aacgttgcaa aattacggaa ttttttccgt
    22381 aataaaggag aaaaaattag aattttttta ttttcagaga ctgcaccaga aagttaccag
    22441 aaaagggacg gaaaagtcca tgataaatcc gtgataaatc cgtgataaat ggaaaccaac
    22501 gcataatcct ttccggcact ttgaacaatc accgggaaag tggtatccat agtagtagcg
    22561 ccacctgaag aaatgggaga aagttttcca aaatatttta ccatgagagg agcgaaaagc
    22621 aaagtgaaaa tttcacgcac gatattggac aacaaagcaa tagtccccaa ctctacgcct
    22681 ttgtagcgag tgatcaggat actggaaagc gaataataac caaatcccga acttgctgcc
    22741 agagagtcga acagagaaaa gccgtcaatg aagaaaccga ctgccagcgc acccaaccac
    22801 gtaccggcaa tggtaaacag aggtaaaaag acaatgcgtg ggttcaagcg acgaaattga
    22861 cggacaaaat tgccttgctg tccgatactg aatccaacgg taaacatcaa acagcaaagc
    22921 acataaaagc tgatttcttc gttaaagaga gtttcgggaa gcaacctgaa aatgcctaaa
    22981 agcaagcctg ttgcaaagaa agacaaaata atcaggctac ttttcatggg tcgatttatc
    23041 cggttttatc catttccaca acaaatctgc tccgagtata cttcccaaag ttccggccgt
    23101 tccaattaac agtgcatcaa agccgagttt tcccgtttga cgaataactg caggattact
    23161 gccaatttct gctcccaata agaaaagcaa cagccagatt aaaacaaaca aaaccgattg
    23221 aataattttg aaatgttttt ttcgaaaaat aaagccgact accataccgg ccaacataca
    23281 acctaataca gtaaacattt gtaaatcatg cgaattatga aatcaattta acaactttta
    23341 ttcaaaaact tttttcatca atatatttgg ggcttaatta aaactttggt aaattatcac
    23401 aaaaagtgat ttttgtattc tatttttaac aaacaactga aaatcaacaa cattgctgta
    23461 aagagcagtt ttcttccgtt gtttcaaatt cgatagtggc atgttcaata ccttgttgaa
    23521 gcaattctga gcgaatttta ttctttattt ctacagcttt ttctattgta actggttgtt
    23581 tcaaaacgac atgaacagta agaatattat aatttccatc aatagaccaa atatgcaaat
    23641 cgtgaatttc atccacattt tcaatcgaca gaagcaattg tgaaattttt tccaggtcga
    23701 catccgttgg cgaaccctga agaagaatgt gcaaactttc ctttacgttg cgaaaaacat
    23761 gaaacaaaat gtaaattgta atcaaaacgg ataaaacagg atcaataacc ggcaaatcaa
    23821 agaaatacat aatcagagaa cccaccagaa tagcagccca tcccagcaca tcttccagca
    23881 tatggagaga aacaaccttc tcgttgatag aacttccttt tttgagacga agaacagcca
    23941 aaccgtttac tagaattcct atcactgcaa aaacaatcat tccttgagtg ttggttgttt
    24001 cgggatgcaa aagacgcggt atcgcttcgt aaaggacaaa aatgctaccc gtaatcaaaa
    24061 taatggaatt gataatggca cccaataaag aaaatctttt gtatccgtag gtgtaatttt
    24121 tatcactttc ttttgtggat tttttttgaa aataccatgc taaacccaat gaaaggctat
    24181 cgcccatatc gtgtatggca tcggaaataa tagcaatact gttggtgaaa attccaccaa
    24241 aaatttcaaa aacggtaaat ccgaaattaa gaaaaaaagc catttttatg ttgctctctc
    24301 catgtgtatg cgtatgttga tgatgatgtt ccattacttg taaattgaaa tttattaaaa
    24361 acaattgaga ttataaatgg ttcgtttttt gcattgaaaa aagttcgaca aaatgatgat
    24421 tgaatctcct cttaaaacct gattttttca aaaaagagaa aaaataagat ttttttgaag
    24481 agaggcatta aattttttgt tgttatatta gcccttaaaa aaaacctgat gaaagcattg
    24541 ggcataccat tgttattgca ataaagaggc ttgaaaattt cttcatcgat gtgtatgaga
    24601 acttctcttc ggaaaagatt gtgccaggaa tcttcccttt gataataatt cccagctgtt
    24661 ccctctcata agttcttcgg gcgaaaaaaa taaatctaat tgtacttcac tttctgtttt
    24721 cctgaacatt tatctctctt ttttatcgta ttttaacatg ctaaggtgct gaaatatagc
    24781 aatatatgca aattttcaaa gaattttttt gaatattttt ttaacttttt tatctcaatg
    24841 tctttaaaaa aactaattct ctttgaataa cttttcggag ttgattctta atttgtggtg
    24901 taaaaatttg aattttcaat aagaaaatct acagaatgac taa
//
LOCUS       DAOTAX010000027        24823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1650_length_24823_cov_31.609173, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000027 DAOTAX010000000
VERSION     DAOTAX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 24823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1650_length_24823_cov_31.609173"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..462
                     /locus_tag="PLM04_04485"
     tRNA            390..462
                     /locus_tag="PLM04_04485"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:423..425,aa:Phe,seq:gaa)
     gene            complement(671..2035)
                     /gene="miaB"
                     /locus_tag="PLM04_04490"
     CDS             complement(671..2035)
                     /gene="miaB"
                     /locus_tag="PLM04_04490"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HON02367.1"
                     /translation="MGLKENTHTSVTENPHKKLYIETYGCQMNVADSEVVASIMQMDG
                     FVLTEDFSEADAVLINTCSIRDNAEQRVFERLKYFHSLKRKNKQLILGVIGCMAERMK
                     EELIEKQGVDLVAGPDAYLDLPNLMGAVEKGEKAINIQLSRTETYQEVIPARLGGQIS
                     GFISITRGCDNFCSYCIVPYTRGRERSRDVESILNELKDLQQKNFKEVTLLGQNVNSY
                     RVEQDGKLIGFPELLEIVAQAAPEMRFRFTTSHPKDMSDRTLETIARYPNLCRFIHLP
                     VQSGSNKILRLMNRKYTREWYLERIAAIRRILPDAAIGTDIFCGFHDETEEDHKQTLS
                     LMREVAFDMAFTFKYSERPGTYAARHLPDNVSEEVKLRRLNEIIALQNELSLESNLRD
                     VGKTFQVLVEGYSKRSKEQFFGRTSQNKVVIFPRQGRRIGETVKVKINSASSATLFGE
                     VEHL"
     gene            2551..3585
                     /locus_tag="PLM04_04495"
     CDS             2551..3585
                     /locus_tag="PLM04_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02368.1"
                     /translation="MVKWIRGFFLLGLFIPFSLQSQVLKGELGLNYFFDNTEFSSSTL
                     TIDQTMKGVHLIPGVAVEWDKNNSLHGGTDLLNISGSQKTIEKIDLVAYYQFQSRKTL
                     FRAGSFPRQNILSNYSDFFFKDSIRYFRPNLQGLFWQVSNPTSFFNLWLDWTGHQTAT
                     QRESFFIGASANKRQHLFFADFQSYMFHYANTAPRNPEYHVCDNLLAHLSAGVDLTSL
                     TQLDTLMFAVGILEGFERERGMDNMTFTPSGLVVRNEIEYGKVGVSNTLYWGDARMKL
                     YEKHGWNLYWSNPFFRSGSYFESKWYWNMLKSRQVNGKLAMEFHVSEGKVFFEQLFTL
                     SASVDKLLKK"
     gene            3640..5178
                     /locus_tag="PLM04_04500"
     CDS             3640..5178
                     /locus_tag="PLM04_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HON02369.1"
                     /translation="MLVKTFGAAVQGINATIITIEVNVNQGIRFFLVGLPDNAVKESH
                     ERIASALDYIGYKMPHKQVVINMAPADIRKEGSAYDLPLAIGILAADGKINPDVLQNY
                     VIMGELSLDGSLQPIKGVLPISIKAREEKFKGFILPKQNAREAAVVNNLEVYGVENLK
                     EVIDFLNGKNQLEPTVVNTREEFFNSLNQSEVDFAEVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKSMLAKRIPTILPPLTLHEALETTKIHSVAGNIDSNTSLIAERPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRTVLEVLRQPLEDRKICISRAK
                     FTIEYPASFMLVAAMNPCPCGYYNHPEKECVCAPGVVQKYLNRISGPLLDRIDIHIEI
                     VPVPFEKLSEKRDAESSAQIRERVIKARQIQELRFRDIDGVYCNAQMTTKLQRMFAVP
                     DKEGLELLKNAMQRLNLSARAYDRILKVSRTIADLDDSEKILPYHIAEAINYRNLDRE
                     GWAG"
     gene            complement(5219..5292)
                     /locus_tag="PLM04_04505"
     tRNA            complement(5219..5292)
                     /locus_tag="PLM04_04505"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5256..5258),aa:Asn,seq:gtt)
     gene            5440..6333
                     /locus_tag="PLM04_04510"
     CDS             5440..6333
                     /locus_tag="PLM04_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HON02370.1"
                     /translation="MSRFKSLSLYASIVAAMLIWAFSFIWTKEALTSFRPMLLITCRL
                     IIASVLMLIVSILSGRFQKIQKQDLKWFLLLALFEPYLYYIGETFGLTMVSSTLASVI
                     ISTIPVFSPLLAFLFLKERIGWFNVAGIILSLTGVFLVIFEPSGNFHVQPLGIVLLFF
                     AVFSAVCYAVVLQKISIHYYTLNIILYQSLFGLMFFIPTTLLADYPYFSTLHFTLSSI
                     EALLMLSIFASVIAYVLFARVVRRIGVARSNVFTNLIPVFTAIFSWLLLNEQLTILKW
                     IGIATVVGGLFVSQARKIKFK"
     gene            6512..7234
                     /locus_tag="PLM04_04515"
     CDS             6512..7234
                     /locus_tag="PLM04_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HON02371.1"
                     /translation="MKINNFLFSVYQWIICVPIVIVLTLLTSIFTIILSPIFPNSNIS
                     YLPARWWSKATCGLFFIKVKVKGLEKIERGKSYVFAANHQSMFDIFVIYGWLPVRFKW
                     IMKKELRKVPFVGLASEMAGHIFVDRSNAQAAKLSIEKAEKQLKKGNSIVIFPEGTRS
                     TNGQMRKFKKGAFQIAADLALPIVPVTIKGSFERLSKTSFKVKPGVIEMIIHQPIDVT
                     PYLPDRIPELMKTSWEMIHSVL"
     gene            7405..10266
                     /gene="uvrA"
                     /locus_tag="PLM04_04520"
     CDS             7405..10266
                     /gene="uvrA"
                     /locus_tag="PLM04_04520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HON02372.1"
                     /translation="MNNTNTDTSEKIEVIGARVNNLKNIDVSIPRDALTVITGLSGSG
                     KSSLAFDTIYAEGQRRYIETFSAYARNFLGNLQRPDVDKITGLSPVISIEQKTVNKNP
                     RSTVGTTTEIYDFLRLLFARAGEAFSYVTGEKMVKYSEDQIVELILKQYEGKRTYLLA
                     PLVRGRKGHYKELFEQIRKKGFLHVRIDGEMKELVPGLRVDRYKNHNIEVVIDRLVVA
                     EKDRRRLKDSMQKAMQQGNGIVMLLEKETDKVRYFSKKLMCPTSGISYDDPDPHHFSF
                     NSPHGACPTCKGLGFVNQVDMDKIIPDKSVSIYNGGIAPLGKYRNTVIFWQIEAILEK
                     YGCQLKTPVKDIPEEAIDEIMNGSEEPLMIKNHSLGITNYSLNYDGILKYIEMQQDDR
                     ASATEQKWANQYSKSVKCPECNGARLRKESLHFLIHEKNIADLAAMDIVELLNWLGNV
                     ENYLTEKQKAIATEILKEIRTRLQFMVDVGLHYLSLGRSSESLSGGESQRIRLATQIG
                     SQLVNVLYILDEPSIGLHQRDNFRLINSLKQLRDSGNSVIVVEHDKSMMLAADYIVDM
                     GPRAGRLGGEVVFAGKPEEMLKAKTLTSAYLNGEKRIEIPARVRKGNGKFLTIIGAKG
                     NNLKNIDVSFPLGVMICITGVSGSGKSSLITETLQPILSQHFYRSLQDPLPYDRMEGL
                     ENIDKVVEVDQSPIGRTPRSNPATYTNVFSDIRNLFATLPEAKIRGYKPGRFSFNTAG
                     GRCETCSGNGYKTIEMNFLPDVYVVCETCNGKRYNRETLEVRFKGKSIADVLDMTINQ
                     AVEFFENQPSILNKIKTLQDVGLGYIKLGQSSTTLSGGESQRVKLATELSKRDTGKTL
                     YILDEPTTGLHFEDIRALLDVLNRLVDKGNTVIIIEHNMDVIKSADYIIDLGPEGGNA
                     GGKVICTGTPAEITRHPESETGKYLKMELDENFMEDLID"
     gene            10286..10504
                     /locus_tag="PLM04_04525"
     CDS             10286..10504
                     /locus_tag="PLM04_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02373.1"
                     /translation="MNKPTLKSLLKTLLYGLGVFGVYIVIIVLLRLFSGRVPDEAVIF
                     NFFTKNDLLIGLLLACILTFTHQRNKKI"
     gene            10594..11760
                     /locus_tag="PLM04_04530"
     CDS             10594..11760
                     /locus_tag="PLM04_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HON02374.1"
                     /translation="MKNQRYDLRGVSASKEDVHNAIRNLDKGIFPKAFCKIIPDILGN
                     NPDYCNIMHADGAGTKSSLAYAYWKETGDLSVWKGIAQDALIMNTDDLLCIGAVDNIL
                     VSSTIGRNKNLIPGEVVSAIINGTDELIQQLGEMGVKLYFTGGETADVGDLVRTIIVD
                     STVTCRMKRADVIDNANIRPGDVIVGLSSSGQASYEKEYNGGMGSNGLTSARHDVFAH
                     YLAEKYPECYDDKIPSELVFSGSYHLTDKIENLGIDAGKLVLSPTRTYAPIVKKMLEK
                     LRPQIHGMVHCTGGGQTKILHFIENLRVIKNNLFPLPPLFQIIQQESQTDWKEMYKVF
                     NMGHRMEIYLPEAYAEEVIDISRSFNVEAKIIGYCEPSDKKELIIESEFGKFEY"
     gene            complement(11823..12398)
                     /locus_tag="PLM04_04535"
     CDS             complement(11823..12398)
                     /locus_tag="PLM04_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HON02375.1"
                     /translation="MDFSLIQIASAFMVLFAIIDILGSVPIILAIKSKGENVHAGKTS
                     LVALVILVVFLFAGDWILQLFNVDIESFAVAGSFVIFIFSLEMILDVEIFRNKGPEGA
                     SSVVPIAFPLIAGPGSFTTLLSLRAEYAIENIIIALVLNICFVFFVLRFTSKIEKWMG
                     KGGIYILRKFFGIILLAVSVKLFTTNIRFLL"
     gene            complement(13084..14775)
                     /locus_tag="PLM04_04540"
     CDS             complement(13084..14775)
                     /locus_tag="PLM04_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HON02376.1"
                     /translation="MKINEEETPKKNLNFIEAIVDQDVKEGKNGGRVQTRFPPEPNGY
                     LHIGHAKAICLNFDIAKRYGGICNLRFDDTNPVKEDVEYVDSIMEDIQWLGYQWKNVY
                     YASDYFQKLWDLAVQFIKKGLAYVDEQSAETIAKQKGTPTVPGIESPYRNRPIEENLA
                     LFQKMTNGEIPEGGMVLRAKIDMASPNMHFRDPIMYRIITSHPHHRTGWQWKVYPMYD
                     FAHGQCDYFEGVTHSLCTLEFEVHRPLYDWFIDQYQDTDYRPRQIEFNRLNLTYTVMS
                     KRKLLQLVQEKFVSGWDDPRMPTLCGLRRRGFTPESIHNFIDKIGYTKFEALNDIGLL
                     EHAARESLNKTAMRVNAVLHPVKLIVTNYPEGKVEMMTTQNNPEDPEAGTREVVFSRE
                     LFIERDDFMENPPKNYFRLMPGQEVRLKSAYIVKCTGCKKDENGVVTEVYCEYDPMTK
                     SGMPESNRKVKGTLHWVSAAHCVEAEVRLYDRLFNVENPSADERDFRELLNPDSLQIL
                     TGCKLENSLKDAKPLEHFQFQRIGYFTVDLDSTPDKLIFNRTVSLKDSWSKELNK"
     gene            complement(14831..15610)
                     /gene="lpxA"
                     /locus_tag="PLM04_04545"
     CDS             complement(14831..15610)
                     /gene="lpxA"
                     /locus_tag="PLM04_04545"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HON02377.1"
                     /translation="MKQPLAYIHPDAKIAPSVVIEPFVTIDKNVEIGEGTRIGPNVTI
                     LEGARIGKNCTIFPGAVIAAIPQDLKFKGEDTLAIIGDNTTIRECVTVNRGTASKGKT
                     QIGDNCLIMAYCHIAHDCFVGSNVIMSNATQLAGEVFIDDYAVISGGVLVHQFTHIGS
                     HVIIQGGTKVNKDVPPYVKAGREPLSFEGINSVGLRRRGFTNEQIGFIQEIYRYIFLS
                     KMNVSNAIDRIEAELPATKERDDILLFIKNSQRGIIKSFLE"
     gene            complement(15635..17017)
                     /locus_tag="PLM04_04550"
     CDS             complement(15635..17017)
                     /locus_tag="PLM04_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HON02378.1"
                     /translation="MTRQRTLKESFTLEGKGLHTGLDIVITFLPAPENHGIKLKRIDL
                     EEPVIIEALAENVTHTVRGTVLQKGDVQVSTVEHALAALYAFGIDNCLIEVNAPEFPI
                     LDGSARFYAEKIKEVGIIEQKAEKDFYVVRKKIEYSLPDSNSKITLLPDDVFSVDVHI
                     GFDSAVLANQYASLDSLDKFADEIASARTFVFVHEIEPLLSMNLIKGGDLKNALVIYD
                     KELSQDTMDRLTDKLHQPRIDATKLGYINGELNYPNEPARHKLLDVIGDLSLIGKPLK
                     AKVIVQYPGHKINTNLAKIIRKEIKKQEVSAPVYDDSVPPVLDINAIRKLLPHRWPFL
                     LVDKIIDINERVIVGVKNVSGNETFFMGHFPNEPVMPGVLLVEAMAQTAGILVLNKVE
                     EPEKYSTYFMKIDNVKFRQKVVPGDTVIFRLEMMDEIRHGCTDMKGYAFVGGKIVAEA
                     QFMAQIIKNK"
     gene            complement(17078..18130)
                     /gene="lpxD"
                     /locus_tag="PLM04_04555"
     CDS             complement(17078..18130)
                     /gene="lpxD"
                     /locus_tag="PLM04_04555"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HON02379.1"
                     /translation="MEFTAQQIADFLGGQVEGDPSVKVSNFSKIEEGKPGTITFLSNP
                     KYSHYIYDCNASIVLVNKDFKPEKEIPATLIRVNNAYECLAQLFSLVEQSKPKKKGIS
                     PLAFIASTAQIGEDVYIAPFVYVGENVKIGKNTQIHSHSNIEDNSSVGENTILFSGVH
                     VYPDCSIGNNCILHSGVVIGADGFGFAQSEDGTYKKITQIGNVVLEDDIEVGANTTID
                     RATLGSTIVHKGVKIDNLVQIAHNDEIGENTVIVAQTGIAGSTKIGKNCVIGGQVGIV
                     GHLQIADNTQIGAQSGVANSVKTPGQIIIGSPAIPIQNFRRSSIVFKNLSDLQKMVIE
                     LQNKVEELEKTKTSEK"
     gene            complement(18245..19513)
                     /locus_tag="PLM04_04560"
     CDS             complement(18245..19513)
                     /locus_tag="PLM04_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HON02380.1"
                     /translation="MTNISGNNSLGESRIQPNKLKIINDPVFGFINIPDDFLYEIIQH
                     PYFQRLNRIKQLGVSFYVYPGAQHTRLQHSLGAMHLMGEAIEELRQKGHVISGEEEQA
                     ARACMLLHDIGHGPFSHALENSLVKGIHHEEISIMLMQQLNKEMNNNLALALEIFSGH
                     YPKKFLHQLVSGQLDMDRMDYLSRDSFFCGVSEGIIGSARIIKMLNVFHGELVVELKG
                     IYSIEKFLVARRLMYWQVYLHKTLVATEKMLTNILKRAKELASVGETLFASPALHYFL
                     YHHLDKQNFVRSTEALDYFVQLDDSDVICAIKVWAHHPDKVLSFLCNAFINRKLFKVE
                     MLQEPLDETKKEELLKKYSRYFDVSQQEAEYFMGEEIVSTDTYDPVDEKINILYKDGT
                     VKDIADASDMLNINVLTKKVYKNYFCYCRI"
     gene            complement(19515..20957)
                     /locus_tag="PLM04_04565"
     CDS             complement(19515..20957)
                     /locus_tag="PLM04_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HON02381.1"
                     /translation="MENLEPSVVWKFFGEITQIPRPSKKEEKIREYLLDVAQQYRLEA
                     KTDQAGNVLICKPATFGYENHKKIILQSHMDMVCEKDGDVQHNFDSDPIQTFIDGDWI
                     KAEGTTLGADNGIGMAMMLAVLTSDNISHPALECLFTVDEETGLTGASAIEDHFLSGQ
                     ILINLDSEDDGVIFIGCAGGIDTTINFPFIAEKSPNNYFSFSLTVKGLKGGHSGDDID
                     KRHGNAIKILNRFLWNVNKKLDLRIHTFRGGNLHNAIPREAEVVASVPFEDKEKLRVL
                     LNEFIAEIEGELKEDEPALQILISSEEIPDQVLDKKSSDKLLNALYACPHGVKAFSRD
                     MPNLVETSTNLASVRMQENNSFLIITSQRSSVESAKFDIAHQVESVFTLAQAQATHGE
                     GYPGWKPNLNSEILQKAKVCYKNITCEEPKVRAIHAGLECGLFLKKYPYLDMISIGPQ
                     MYGVHSPNERLSISSTRKCWQWLLKILEKS"
     gene            complement(21087..21977)
                     /locus_tag="PLM04_04570"
     CDS             complement(21087..21977)
                     /locus_tag="PLM04_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02382.1"
                     /translation="MIDKFFIKRYFLFVFSLILSVSFFSCGEKEENEPDITNSIYVTQ
                     VFEYVYAPGQHAQLAKPADIEHFIGEPSDEKGFLYLGGFGGYVIAGFDHDIQNKDGYD
                     LEIFALPGASPEPAIVYVMPDDNGDGKPNDTWYELKGNQFENEKRNYWVRYYKPATSN
                     DNIQWKDSEGENGELISGFGAPNTSSWWWATCMEDSITFEGSRLPDAYNNKSSESAQS
                     WIVPTDRFIWGYAENNFGTDYDTHHHSNKLDISNAVDSSGKAVYLEKIRFIKIQTAVF
                     QRAGWVNEVSAEIRGAAELK"
     gene            complement(22253..23338)
                     /locus_tag="PLM04_04575"
     CDS             complement(22253..23338)
                     /locus_tag="PLM04_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HON02383.1"
                     /translation="MIKVGDKVRFLNSTGGGIVTRIDEREKIVYVSDADGFEIPMLER
                     ECVVIPEVNENTNFPKKDFKSASAAFMNENESPAAKSINLPPKPEIIEEEIFETPEGE
                     NLRVFLAFVPEDIKQLQTTSNDFYLVNDSNYFLFYNLALFENECCKSVANGLIEPNMQ
                     EWMAEIKKEELNAWEKIRVQIIPFKKHKTYRPQPVIDIDLKLNLMKFYKLHSFGENEY
                     FDVPAMMLNPLEEIETHQLHDISAEEIKQAISQKEKPERRPRISKPAKNPEIVEVDLH
                     INQLVDTTVGMNNADMLELQLEKFESVLNDYKNRKGQKIVFIHGKGEGVLRKEIEKLL
                     RTKYKSYYFQDASFQQYGFGATMVTIR"
     gene            23478..24710
                     /locus_tag="PLM04_04580"
     CDS             23478..24710
                     /locus_tag="PLM04_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HON02384.1"
                     /translation="MNELKEKTNFSIDDFDYVLPEERIAKYPLKKRDASKLLIYRDGT
                     ITENVFSNIASFLPEKALLVYNNTKVIQARIHFRKETGALIEVFCLEPAQPADYALSL
                     SATKECVWKCFVGNQKKWKNGTLSKELDINGTQFEFSAELLERKGNVNFVRFSWNNEN
                     IHFAEILEKAGELPIPPYLHRPTEESDLITYQTVYAKIKGSVAAPTAGLHFTPEVFES
                     LKSRNIETAELTLHVGAGTFQPVKTKQISDHQMHTEVIEIQKKTIEKLLKNIGKIIAV
                     GTTSVRTLESLYHIGLLIEKQQFPDEKHPYFFIPQWMPYQTECVLTPEASLQLILNEM
                     EKRNLSVLHAQTQLIIQPGYTFRLTNGMITNFHQPKSTLLLLVSAFVDGNWRKIYDYA
                     LSHDFRFLSYGDSSLLMK"
BASE COUNT         7514 a         5066 c         4677 g         7566 t
ORIGIN      
        1 cctgtgaatt ctttttacaa catttagaaa tcagaaatta aatgtagaat gtagtttttg
       61 aacaaatgat tgtcatgcaa aatcggtaga tgaatatgat agtcaggatg tagcaaattt
      121 tgggacaaag aaaaaagaag aaactatatg atggacaact tgtatttttc gacaaataaa
      181 atacctcgag gcttgctcta gatgattcat tacattgatt cccaatttta tttacataaa
      241 cacttgatac aggatacttt aaggataatc gttattttga aaaaagtcaa ggttaaaccg
      301 caattttttc aaaaatatga aattttttag ctgagaaact ttgagaataa atagaaaagt
      361 actatctttg cattctcaaa aataaaaatg gtaccatagc tcagttggta gagcaaagga
      421 ctgaaaatcc ttgtgtcccc ggttcgattc ctggtggtac cacaaaaaag cactttaaat
      481 tgtcagattt aagtgctttt tttggttcgc tatttaaaat taggtgtttt aaaaaaattg
      541 gtcaatttaa ttagaaagaa tttgatatcg aatattctta tttcatcctc taaatgactt
      601 catcatagat tcgaccgata aacgccaaac acaaataacc tgaaattaaa aaagtctttt
      661 tcttcagaaa ttacagatgc tctacttcgc caaataaagt tgccgacgaa gccgagttaa
      721 ttttcacttt caccgtttcg cctattcgcc ttccctgacg cggaaaaatc accactttat
      781 tttgtgaagt tcgtccgaaa aattgctctt tggaacgttt ggaatagcct tccaccagta
      841 cttgaaaagt ttttcctacg tcccgcaggt tgctttcgag cgaaagttca ttctgcaaag
      901 cgataatctc attcaaacga cgcaatttca cttcttcgct cacgttatcg ggcaaatgac
      961 gagcagcata agttcccggg cgttcggaat atttaaaagt aaaagccata tcgaaggcta
     1021 cttcccgcat caacgaaagt gtttgcttgt gatcttcttc cgtttcatca tgaaacccgc
     1081 aaaaaatatc tgttccaata gcagcatccg gtaaaattct tcggatagcg gcaatgcgtt
     1141 ccaaatacca ttccctggta tattttcggt tcatcagccg taaaattttg ttgcttccgg
     1201 attgcacggg gagatgaata aaccggcata aatttgggta tcgagcaatg gtttcaagtg
     1261 tgcggtcgct catgtctttg ggatgagaag tggtaaaacg aaaacgcatt tcgggtgcag
     1321 cctgagctac aatttccaac aattcaggaa atccgattaa ttttccatcc tgttccacac
     1381 ggtaggaatt aacgttttgt cccaaaagtg taacttcttt aaaatttttc tgctgcaaat
     1441 ctttcaattc gttaagaata ctttccacat cacgacttct ctctctacct cgcgtataag
     1501 gcacaatgca ataggaacaa aaattatcgc atccacgcgt aatggaaata aaacctgaga
     1561 tttggccccc cagtcgggca ggaatcactt cctgataggt ttccgtacgc gaaagttgga
     1621 tattaatggc tttctcacct ttttcgacgg ctcccatcaa gttgggtaaa tcgagataag
     1681 catcaggccc tgccaccaag tcaacgccct gtttttctat caactcttct ttcatccgct
     1741 cggccataca gccaatcaca cccaaaatca actgtttgtt ttttcgtttc aacgaatgaa
     1801 aatatttaag tcgttcgaac actcgttgtt cagcattgtc gcgaatggag caggtattga
     1861 tcaatacggc atcggcttct gaaaaatctt ctgtcagtac aaagccatcc atttgcatga
     1921 tggaagccac cacttcactg tcagccacat tcatttgaca accgtaagtt tcgatataaa
     1981 gttttttatg tggattttcc gtaacggaag tatgagtatt ttcttttaat cccatgattc
     2041 aagctttaaa aaaatgctta caaaggtaca acattaaagg aaattttatg gttaaaaatg
     2101 ttttagcttt atttaactac atgttaaata gttcattatt taattgattt cttttaattt
     2161 acatcaacag ataaaaaaag aatcgagaaa aaaagaattt ttttttgcaa tcgtttgcaa
     2221 aattcaaaat taagtttcat ctttgcaacc gtaaacaaac agataaattt ttcatagtta
     2281 aggtttaggt taaaaggtac aatgggtggc tgggaagtta cccattgtta ttttgagccc
     2341 cccatcaatc aaggcttatc taaataaaaa tttttgcgtt tatattttga accttacttc
     2401 ttcccattat ctattttcct ataatttttt gtttttagtc ataatattcg ttattcatac
     2461 tcaattttta agaaaaaaca gctaaacccc accaaaatat gaagataatc tgtatttttg
     2521 cttaaaatta attttaagtt gaaaaatttt atggttaaat ggattcgggg tttctttctg
     2581 cttggcctat tcatcccttt ttcccttcaa agtcaagtgt taaaaggcga attgggatta
     2641 aactattttt ttgacaacac cgaattttct tcttctaccc ttaccatcga tcagaccatg
     2701 aaaggcgttc atttaattcc aggagtggct gtggaatggg acaaaaataa ttccttacac
     2761 ggaggcactg atttattgaa catttccggt tctcaaaaaa ccatagaaaa aattgatttg
     2821 gtggcttatt atcaattcca atcacgaaaa actcttttcc gtgccggttc ttttccgcgt
     2881 caaaacatac tttccaacta ttccgatttc ttttttaaag attccatacg atatttccgt
     2941 cccaatttac agggactttt ctggcaagtt agcaatccga cttcattttt taacctttgg
     3001 ctcgattgga caggacatca aacagccacc caacgggaat ctttttttat tggagcgtct
     3061 gccaacaaac gacaacatct gttttttgcc gattttcaat cttatatgtt tcattatgcc
     3121 aacacggcac cacgtaatcc tgaatatcat gtttgcgaca atttgctggc ccatctttcc
     3181 gcaggagtcg atctgacctc tcttacacaa ttggacacgc ttatgtttgc ggtcggaatt
     3241 ttagagggtt ttgagcgcga acgtgggatg gacaatatga cttttacgcc ttccggttta
     3301 gttgttcgaa atgaaattga atatggcaaa gttggcgttt ccaatacgct ttattggggc
     3361 gatgcgagaa tgaaattgta cgaaaaacat ggctggaatc tttactggag caatccattt
     3421 tttcgatccg gcagttactt tgaaagcaaa tggtattgga acatgctgaa aagcaggcaa
     3481 gtgaacggaa aattggctat ggaattccat gtatcggaag gaaaagtatt ttttgaacag
     3541 ttgttcaccc tttcggcttc ggtggataag ctgttaaaaa aataagctat actgcagaaa
     3601 taaaattaat tatctgaatt acacctaaaa atattcctca tgctggtaaa aacttttgga
     3661 gctgctgttc aaggcattaa tgccaccatt attaccattg aagttaatgt aaatcaggga
     3721 attcgtttct ttctggtagg acttcccgac aatgcggtaa aggaaagtca cgaacgcatt
     3781 gcttccgcat tggattatat aggctataaa atgcctcaca aacaagtggt tatcaacatg
     3841 gccccggccg atattcgtaa agaaggttca gcttacgact taccgctggc tatcggcatt
     3901 ttggctgccg atgggaaaat taatcccgat gtgcttcaaa attacgtcat tatgggcgaa
     3961 ttgtcgcttg atggaagttt gcaaccgata aaaggtgttc tgcctatttc catcaaggca
     4021 cgcgaagaaa aattcaaggg atttatcctg cccaagcaaa atgctcgcga agctgccgta
     4081 gtaaataatt tggaagttta cggagttgaa aacttaaagg aagtaatcga ttttctgaat
     4141 ggcaaaaatc aactcgaacc aacagtagta aatacgagag aagaattttt caacagcctg
     4201 aaccagtccg aagtagattt tgccgaagtg aaagggcagg aaaatgttaa aagagcgctc
     4261 gaagtggctg ctgctggcgg acacaatata atcatgatag gtccgccggg cgcaggaaaa
     4321 tcgatgctgg ccaaaagaat ccccactatt cttccacctc tgactcttca tgaagcattg
     4381 gaaacaacca aaattcattc cgttgccggc aatatcgaca gcaacacttc gctcattgct
     4441 gaacgcccgt tccgaagtcc acatcatacc atttcggatg tagcactggt gggcggaggt
     4501 acatttcctc aacccggaga aatttcgctt gcccacaacg gtgttctttt tctggacgaa
     4561 ttgcctgaat ttaaaagaac agtgctcgaa gtgttgcgcc aaccattgga agacaggaaa
     4621 atatgtattt cgagagccaa atttaccata gaatatcccg ccagctttat gctggtagca
     4681 gccatgaatc cctgtccttg cggctattac aaccatccgg aaaaagaatg tgtttgcgct
     4741 ccgggtgtgg tacaaaaata tctgaatcgc atttcagggc ctctgctcga cagaatagac
     4801 attcatatcg aaattgttcc tgtgccattt gaaaaacttt ccgaaaaaag agatgccgaa
     4861 agtagcgccc aaatcagaga acgagtgatt aaggctcgtc aaattcagga actgcggttc
     4921 agggatattg atggcgttta ttgcaatgcc caaatgacca caaaattgca acgcatgttt
     4981 gccgttcccg ataaggaagg gctggaatta ttgaaaaatg ccatgcaacg actcaatcta
     5041 tcggcacgcg cttacgacag aattctaaaa gtttcgcgaa caattgccga cttggacgat
     5101 tcggagaaaa ttctaccgta tcacattgcc gaagccatta attacagaaa cctcgaccgg
     5161 gaaggctggg caggataaaa actgcgcaaa caaaaaaacc acctttgcgg tggttttgct
     5221 cttcctcttg gacttgaacc aaggaccctc tgattaacag tcagatgctc taaccgactg
     5281 agctaaggaa gaatttgata ttgctttcaa aaagcgaagg caaaagtaat attctttttt
     5341 gatatacaca aaaatttccg attgaaattt gatttaaaat tctttgtcga tagaaaaaat
     5401 aaaaacaaaa attaaaataa caagctgatt cttaaatata tgtcaaggtt caaatcattg
     5461 agtttatatg cttccattgt tgctgccatg cttatctggg ctttctcgtt tatctggaca
     5521 aaagaagcgt tgacttcatt tcgacccatg ttgctgataa cctgtcgatt aatcatagct
     5581 tctgtactaa tgctcatcgt ttcgatactt tcaggtcgct ttcagaaaat tcagaaacaa
     5641 gatttgaaat ggtttttgct gctggctctg tttgaaccat acctctatta cattggcgaa
     5701 actttcggtt tgacaatggt ttcttctaca ctggcatcgg ttattatttc aactattccg
     5761 gtattttcgc cgctactggc ttttttgttt ttgaaagaac gcatcggctg gttcaatgta
     5821 gcaggaataa ttttgtcgct taccggtgtt tttcttgtca tttttgaacc aagcggcaat
     5881 ttccacgtcc agccactcgg cattgtttta ctcttttttg ccgttttttc ggccgtttgt
     5941 tatgccgtag ttttgcaaaa aatttccatt cattattaca cgctgaacat tattctttat
     6001 caaagccttt tcggattgat gttttttatt cccactacat tgctggctga ttatccctat
     6061 ttctcaactt tgcattttac cctatcttcc atcgaagcat tgcttatgtt gtccattttt
     6121 gcttcggtaa tagcttatgt tctctttgca agggtagtga gaagaatcgg agtagctcgc
     6181 agcaatgttt ttaccaattt gataccggtt tttacggcta ttttctcctg gctattatta
     6241 aacgaacaat taaccatact taagtggata ggaattgcta ctgttgttgg tggactgttt
     6301 gttagtcagg cgcgtaaaat caagttcaaa taaacaatca accgtacttg tttgtttaat
     6361 ctaaaagctt tttgaaataa acgggaaaca tatatctttg caccgaaaaa ttttcttttc
     6421 ttgctattga tttaatctcg aaaaatttta aaaaagtttt attataaaat tctttgattg
     6481 atttttttct attccaaata atttcaaatc catgaaaatc aataattttt tattctctgt
     6541 atatcaatgg attatatgtg ttcccattgt aattgtttta actttgctga catctatttt
     6601 taccataatt ttatctccaa ttttcccgaa cagcaatata tcttatttgc cggcacgatg
     6661 gtggtcgaaa gcaacctgcg gattattctt catcaaagtg aaagtcaagg gactggaaaa
     6721 aattgaaaga ggaaaatcct atgtttttgc tgccaatcat caaagcatgt tcgatatttt
     6781 tgtaatttat ggctggcttc ccgtacggtt caaatggatc atgaaaaaag aactgagaaa
     6841 agttcctttt gtaggattgg ccagcgaaat ggccggacat attttcgtag accgttcgaa
     6901 tgcacaagcc gcaaaactca gcatcgaaaa agccgaaaaa caattgaaaa aaggaaactc
     6961 catcgttatt tttcctgaag ggacgcgttc aacaaacgga caaatgagaa aattcaaaaa
     7021 aggcgctttc caaattgctg ccgacctggc actccctatt gttccggtta ccatcaaagg
     7081 aagctttgaa agattatcaa aaacgagttt caaagtcaaa ccaggcgtta tagaaatgat
     7141 tattcatcag ccaattgatg taacacctta tctgcccgac cgcattcccg aactgatgaa
     7201 aacttcttgg gaaatgattc attctgtttt ataattttat taaaattttt tactcttggc
     7261 ttattgagaa gaatattttt cgaaaaaaca aaatattcaa tttttgcatt gctttgagtt
     7321 attccaatat ttgaaactac cttcattttt tgtacctttg caggttcaat ttaaaggagg
     7381 ctgactttgt caaacaacaa caatataaat aacacgaata cagatacttc agaaaaaatt
     7441 gaagtcattg gagcacgtgt caacaactta aaaaatatcg atgtttccat tccccgcgat
     7501 gccctcaccg tcatcaccgg attgagtgga agcggaaaat catccctcgc tttcgatacc
     7561 atttatgctg aaggacagcg acgctatatc gaaacttttt cggcgtatgc cagaaatttt
     7621 ttaggtaatc tgcaacgtcc tgatgttgat aaaattacag gtctaagtcc tgtcatttcg
     7681 attgagcaaa aaacggtcaa caaaaatcca cgttctaccg ttggcaccac taccgaaatt
     7741 tacgactttc tccgactact ttttgcccgt gccggtgaag ctttttccta tgttacggga
     7801 gaaaaaatgg taaaatattc ggaagatcag attgtagaac tgattttgaa acagtacgaa
     7861 ggcaagcgca cttacttgct ggcaccattg gtgcgtgggc gcaagggaca ttataaggaa
     7921 ttgtttgaac aaatccgaaa aaaaggattt cttcatgtac gtatcgacgg agaaatgaag
     7981 gaattggttc ccggacttcg cgttgaccga tacaaaaacc acaatataga agttgttatc
     8041 gaccgactgg tggtcgccga aaaagatcgt cgccgactca aggattccat gcaaaaagcc
     8101 atgcagcaag gcaatggaat tgtgatgctt ttggaaaaag agaccgacaa ggtacgttat
     8161 ttcagcaaaa aattaatgtg tccaaccagc ggaatttctt acgatgaccc cgatccgcat
     8221 catttctctt tcaattcgcc tcacggtgcc tgtcccactt gcaaaggatt gggatttgtc
     8281 aatcaggttg acatggacaa aattattccc gataaatcgg tttccattta caacggcggt
     8341 atagctccat tgggaaaata cagaaatacc gtgatttttt ggcaaataga agcaattttg
     8401 gagaaatacg gctgccaact gaaaactcct gtcaaagata ttcccgaaga ggctatcgac
     8461 gaaatcatga atggctcgga agaaccttta atgattaaaa accacagtct gggtatcacc
     8521 aattattcct tgaattatga cggtatttta aaatatatcg aaatgcagca ggatgacaga
     8581 gcttctgcca ccgaacagaa atgggcaaat caatattcca aatcggtgaa atgtccggaa
     8641 tgcaatggag ccagactgcg aaaagaatcc ctccacttcc ttattcacga gaagaacatt
     8701 gccgaccttg ccgcaatgga tattgtcgaa ttgttgaatt ggttagggaa cgttgaaaat
     8761 tatcttaccg aaaaacaaaa agccatagcc accgaaattt taaaggaaat tcgtacccgt
     8821 ttgcagttca tggtcgatgt agggttacac tatctttctc tgggtcgaag ctccgaatcg
     8881 ctttcaggtg gagaaagtca gcgaatcagg cttgcaacgc aaattggttc acaactggta
     8941 aatgtgttgt atattctgga cgaacccagc atcggactgc atcaacgcga taattttcgg
     9001 ctgatcaatt cattgaaaca actgcgcgat tccggaaatt cggttattgt agtggaacac
     9061 gacaaaagta tgatgttggc tgccgattat atagtcgata tggggccacg agccggtcgc
     9121 ttgggtggag aagtagtttt tgccggtaaa ccggaagaaa tgttgaaagc caaaacattg
     9181 acttctgcct atctgaatgg agaaaaacgt atagaaattc ctgctcgtgt gcgcaaagga
     9241 aacgggaaat ttctgaccat tatcggggcc aaaggaaata acttgaaaaa tatcgatgtg
     9301 tcgttccctt tgggcgtcat gatttgtatt accggagttt caggtagcgg aaaatcttca
     9361 ttaatcacgg aaacccttca acccatattg agccagcatt tttaccgttc gttacaggat
     9421 ccgcttcctt acgaccggat ggaaggattg gaaaatatcg acaaagtcgt ggaagttgat
     9481 caatctccta ttggacgcac gcccagatcc aatccggcaa cctatacaaa cgttttttcg
     9541 gatattcgaa atttatttgc aactttgcct gaagctaaaa ttcgcggcta caaacccggt
     9601 cgattttcat tcaacactgc cggcggtcgt tgcgaaacat gctccggaaa cggttacaaa
     9661 actattgaaa tgaattttct tcccgatgtt tacgtggttt gtgaaacctg taacggcaaa
     9721 cgctacaaca gagaaacact ggaagtccga tttaagggaa aatccattgc cgatgtgttg
     9781 gacatgacca ttaatcaggc ggtagaattt tttgaaaatc agccatccat tctcaataaa
     9841 ataaaaacac ttcaggatgt aggacttggc tatattaaac tcggacaatc cagcaccacc
     9901 ctttcaggag gagaaagtca acgcgtgaag ctggcaaccg agctgtccaa acgcgatacg
     9961 ggaaaaacac tttatatttt ggacgaaccg accaccggct tgcactttga agatatacgc
    10021 gctttgctcg atgttttgaa tcggctggtt gataaaggaa atacggtgat tataatcgaa
    10081 cacaacatgg acgttatcaa aagtgccgat tatatcatcg acttgggccc tgaaggcgga
    10141 aatgccggcg gaaaagttat ttgcaccgga actccggccg aaataactcg ccatcccgaa
    10201 agcgaaacgg ggaaatatct taaaatggaa ctggacgaaa attttatgga agatttgatc
    10261 gattgaaaaa ttaaactcat aaattatgaa caaaccaaca ctgaaatcgc tgttgaaaac
    10321 gcttctttac ggacttggag tatttggagt atatattgta attattgttt tactccgact
    10381 tttcagtggt cgcgttccgg atgaagctgt gatattcaac ttttttacca aaaacgattt
    10441 gttgattggt cttttattgg cctgtatcct gactttcacc catcaaagaa acaagaaaat
    10501 ataatttttt gacatgagtt gaagttctta tttcttgcga aaattaaacc gatgatttgc
    10561 aaataaaata ttaaaaatcc atttaaaact gttatgaaaa accaacgtta cgatttacgc
    10621 ggagtttcag catcaaaaga agatgtccac aatgcgatcc ggaatttgga taagggaatt
    10681 tttccaaagg ctttttgcaa aatcattccc gatattctgg gaaacaatcc cgattattgc
    10741 aacatcatgc atgctgacgg agcaggcaca aaatcttcgt tggcttatgc ctactggaaa
    10801 gaaacaggcg atttgtcggt atggaaaggt atcgctcagg atgcgctaat catgaatacc
    10861 gacgatttgt tgtgtatagg agcagtggat aacattttgg tttcctccac cattggaaga
    10921 aacaaaaacc tgattcccgg cgaagttgtt tcggctatca tcaacggaac ggacgaactg
    10981 attcaacaac ttggcgaaat gggcgtaaaa ctgtatttca caggaggaga aactgccgat
    11041 gtgggcgact tggttcgaac cattattgtg gacagcactg taacttgccg aatgaaaaga
    11101 gccgatgtaa tagacaatgc caatattcgt cccggcgatg taattgtggg gctttcctca
    11161 agtggtcagg ccagttacga aaaggagtac aacggcggca tgggcagcaa tggtttgaca
    11221 tccgcccgac atgacgtttt tgcacattat ttggccgaaa aatatcccga atgctacgat
    11281 gacaaaattc cttccgaact ggttttttca ggttcttacc atttaaccga caaaattgaa
    11341 aacctcggta tcgatgccgg gaaactggtg ctttcaccta cccgaacgta tgctccgatt
    11401 gtaaaaaaaa tgctggaaaa acttcgtccg caaattcatg gaatggttca ttgtacggga
    11461 ggaggtcaga caaaaatttt acattttatt gaaaatctca gggtaattaa aaataacctt
    11521 ttccccttgc cgccgctttt tcaaatcata cagcaagaat cccaaaccga ttggaaagaa
    11581 atgtataagg ttttcaatat gggacatcgc atggaaattt atctgcccga agcttatgca
    11641 gaagaagtca ttgacatttc ccgctcattc aatgtcgaag caaaaattat cgggtattgc
    11701 gaaccatccg acaaaaaaga attaatcata gaaagcgaat tcgggaaatt tgaatattga
    11761 tagcagcaag ataatttctg ccaatatatt ttattccaaa aaatgccgaa aaaacgcgcc
    11821 aactacagca aaaatctaat gttggtagta aatagtttta ctgaaacggc gagcaaaata
    11881 atgccgaaaa atttccttaa aatataaatt cctcctttcc ccatccattt ttctattttg
    11941 gatgtgaacc gtaacacgaa gaaaacaaaa caaatgttca gcacgagcgc tattataatg
    12001 ttttcgatgg catattccgc gcgtaaggaa agtaatgtgg taaacgaacc tggccccgct
    12061 atcaatggaa aagcgatcgg cactaccgaa gaagctcctt ccggcccttt gttcctgaaa
    12121 atttccacat cgagaatcat ttccagcgaa aaaataaaaa tcacgaaaga acccgcaaca
    12181 gcaaaagatt caatatcgac attgaaaagt tgaagaatcc agtcaccggc aaataaaaaa
    12241 accaccagaa tgaccaaagc caccaagctg gtttttccgg catgaacatt ttcaccttta
    12301 cttttgatag ccaaaataat aggcaccgag cccaaaatat caataatggc aaacagtacc
    12361 ataaaggcac tggcaatttg tattaaactg aagtccatag ttttaattgt ttgttgagtt
    12421 ctatttctta atgaaacagt ttcaatttaa agccgaatga atttttcccc tatttgaata
    12481 aaaagtccac gttaaatctt ctgtcccgct catcgggtgc aagttctact tttacaggta
    12541 gtatattttg aagttgtcca taaatagttt aactttgggt tacataacca tcataaattc
    12601 tgtcaattac ctgttgtatc atgtaatcga tggaatcctg ttcggttatt tcggcaactt
    12661 ctgcatggat cacttcagtt atctaaataa aagcgttttc ccaagttcaa taatatattc
    12721 ttttgacccg agattatcga aataatattc acgccattct tccaaatttt tttgtgcaca
    12781 ttttcgaatc gactcggatg agctccaaca ttctttaaga aatttaattg gaatcgattc
    12841 attgcgctat tgaatatgct ttctttatca tttatagatc cgaaatacag cactataagt
    12901 taatgaactt tttcactcaa gttcgttctc tttattcgca attcatctgc gaatataaca
    12961 cagaattctc aatttagaaa tttattgcat aaaaaattcc tgaattccaa tccagaccac
    13021 tacaaaagtc aacaatgctg caaatattca ttacaacatt cttggcaagt atttgaggtg
    13081 attttactta ttgagttctt tactccaact gtccttgaga gagacagtgc gattgaatat
    13141 caacttgtcc ggagttgagt ccaaatcgac tgtaaaatat ccaatgcgtt gaaactggaa
    13201 atgttcgaga ggcttggcat ctttaagtga attttccagt ttgcaaccgg taagtatttg
    13261 taaggaatcg ggattgagta attcgcgaaa atcgcgttcg tcggcagaag ggttttcaac
    13321 gttaaataag cggtcgtaga ggcgtacttc agcttccaca caatgagcag ccgaaaccca
    13381 gtgaagcgtt cccttcacct tgcgattgct ttcaggcatg ccgctttttg tcatggggtc
    13441 atattcgcaa taaacttcgg taacaacacc attttcatct tttttgcaac ccgtacattt
    13501 aacaatataa gcacttttca gccgaacttc ctgaccgggc atcaggcgaa aataattttt
    13561 tggaggattt tccataaaat cgtctctttc aataaaaagc tctctcgaaa agacaacttc
    13621 tctggttccg gcttcagggt cttcaggatt attttgcgtt gtcatcattt ctacttttcc
    13681 ttcaggataa ttggtaacaa tcagcttgac gggatgaagt acggcattaa cacgcattgc
    13741 cgttttgttg agactttcgc gggcagcatg ttcaagcaat ccaatatcgt tcagcgcttc
    13801 aaattttgta tagcctattt tatcgataaa gttatgtatg gattcaggtg taaaaccacg
    13861 tcgacgcaaa ccacaaagtg ttggcatgcg gggatcgtcc cagccactga caaacttttc
    13921 ctgaacaagt tgcaaaagtt tacgcttgct catgacggta taagtaagat tgagacgatt
    13981 gaattctatt tgtcgcggtc gataatcggt atcctgatac tgatcgataa accagtcgta
    14041 aagtggtcga tgaacttcaa actcaagcgt acataaagaa tgagtaactc cttcaaaata
    14101 atcgcactga ccatgagcaa aatcatacat cggataaact ttccattgcc aacccgtacg
    14161 gtgatgcgga tgggaagtaa taatcctgta catgatggga tcgcgaaagt gcatattggg
    14221 agaagccata tcgatttttg ccctcaacac catgccgcct tcgggaattt ctccattggt
    14281 cattttttga aaaagcgcaa gattttcttc tatcggacga ttccgataag gactttctat
    14341 tccgggaaca gtaggtgtac ctttttgttt ggctatggtt tcggcacttt gttcgtctac
    14401 ataagccaac ccttttttga taaactgaac tgctaaatcc caaagtttct gaaaataatc
    14461 ggaagcgtag taaacatttt tccactgata acccaaccat tgaatgtctt ccattatgga
    14521 atcgacatat tcaacatctt ctttcacggg gttggtatca tcaaaacgaa gattacaaat
    14581 tcctccgtat ctttttgcaa tatcgaaatt cagacaaata gccttagcat gtccgatatg
    14641 caaataaccg ttcggttcgg gaggaaacct tgtctgaaca cgtccgccat ttttcccttc
    14701 cttaacgtcc tgatcaacaa tagcttcaat gaaattcaga tttttttttg gagtttcttc
    14761 ttcgttaatt ttcatagctt ctgaatgata taaaaaattt tttctcaaaa acagagaaat
    14821 gaaatcaagt ttattccaga aaacttttga taatccctct ttgcgaattt tttatgaaaa
    14881 gaagaatgtc atcacgttct ttggtagcgg gcaattcggc ttcgatacga tctatggcat
    14941 tgctcacatt cattttcgac aaaaagatat agcgataaat ttcctgaata aaaccaattt
    15001 gttcattggt aaatccgcgt cgccgcaaac ccaccgaatt gattccttca aatgacagcg
    15061 gctcacgccc tgctttcacg taaggtggta catccttatt cacttttgtt ccaccctgaa
    15121 taattacatg cgacccaata tgggtaaatt gatgcaccaa gacgcctcca ctgatcaccg
    15181 cgtaatcatc aatgaatact tcgccggcaa gctgcgtggc attggacata atgacattgc
    15241 tgccaacaaa gcaatcatgt gcaatgtggc aataagccat gatcaggcaa ttgtcgccaa
    15301 tttgggtttt tccttttgaa gctgtgcctc tgtttacggt aacacattcg cgaatggtgg
    15361 tattatcgcc aataatggcc aaagtatctt cacctttgaa tttcaaatcc tgtggaatag
    15421 ctgcaattac ggcaccggga aaaatagtac agtttttccc gattcgggct ccttccaaaa
    15481 tggtaacatt cggaccgata cgcgttcctt cgcctatctc cacattttta tctatggtaa
    15541 caaatggttc aatgactacc gacggtgcaa tttttgcatc cggatgaata tatgccagcg
    15601 gttgtttcat tgtatgggtg taaatctttt agttttattt gtttttgatg atttgtgcca
    15661 taaactgggc ttcagcaaca atttttccgc ctacaaaagc gtaacctttc atatcggtgc
    15721 atccatgacg aatttcatcc atcatttcta atcgaaaaat aactgtatcg cccggtacaa
    15781 ctttctgacg gaattttaca ttgtctattt tcataaaata agtagaatat ttttcgggtt
    15841 cctccacttt gttcaacacc aaaattccgg cagtctgcgc catagcttct accagaagaa
    15901 ctccgggcat aacaggttcg ttgggaaaat gtcccataaa aaacgtttcg ttgccggata
    15961 catttttcac acccacaatc actctttcgt tgatatcgat aattttatcg accagcagaa
    16021 aaggccagcg atgcggcaaa agttttctaa tagcattaat gtccaatacg ggcggaaccg
    16081 aatcgtcgta cacaggagcc gaaacttcct gcttttttat ttccttgcga atgattttgg
    16141 ccagattggt atttatttta tgaccgggat actgcacaat aacttttgct ttcagtggtt
    16201 tcccaatcag ggaaaggtct ccgataacat ccagtaattt atgtcgagca ggctcattgg
    16261 gataattcag ttcaccgttg atatatccaa gtttggttgc atcaatacgt ggctgatgaa
    16321 gtttgtcggt gagtctgtcc atcgtatcct gcgacaattc cttatcgtaa atgacaagtg
    16381 catttttcaa atcgccacct ttgatgagat tcatgctcaa gagcggttca atttcgtgaa
    16441 caaacacaaa agtacgagca gaagcaattt catcggcaaa tttatcgagt gaatccaaac
    16501 ttgcatactg attggcaagt acggcagaat cgaatcctat gtgaacatct acactgaaaa
    16561 catcatcggg caacaaagtg attttggagt tggaatcggg aagtgaatat tcaatttttt
    16621 ttcttaccac ataaaaatct ttttccgctt tctgttcaat aattccgacc tctttaattt
    16681 tttctgcata aaagcgggca cttccatcca aaatggggaa ttcaggtgca ttgacttcta
    16741 tcaggcaatt gtcgatacca aaagcataaa gcgcagccaa cgcatgttct accgtgctta
    16801 cttgaacgtc gcctttttgt agaacagttc cacgtaccgt gtgggtaaca ttttctgcca
    16861 atgcttcaat gataacgggt tcttccagat caatacgttt taactttatt ccatgatttt
    16921 ctggagccgg caaaaaggta atcacaatat ccaaacctgt gtgtaagcct tttccttcta
    16981 atgtaaaaga ttcctttaat gttctttgtc tcgtcatttg tttttgatta acaattttta
    17041 tgcatttgag gcagtcaaaa aaaaatttca gaaaaaatta tttttctgaa gttttagttt
    17101 tttccagctc ttctacctta ttttgtaatt ctatcaccat tttttgtaaa tcggagagat
    17161 ttttaaatac aatggaagaa cggcgaaaat tctgaatggg aatagcagga gaaccgataa
    17221 taatctgacc gggagttttt accgaattgg caacacccga ctgtgccccg atttgggtat
    17281 tgtcagcaat ctgcaaatgt ccaactatac cgacctgtcc gcctatcaca caatttttcc
    17341 ctattttggt tgaaccggca atgcctgttt gcgctacaat tactgtgttt tcaccaattt
    17401 cgtcgttatg ggctatttgc accagattat caattttcac acctttgtga acaatggtgc
    17461 ttcccaacgt agcgcgatca atggtggtgt tggctccaac ttctatatcg tcttccaaca
    17521 cgacattccc tatttgggta atttttttgt aagtaccatc ttccgactgg gcaaaaccaa
    17581 aaccatcggc accaattacc acaccggaat gcagaatgca attgttgcca atgctacaat
    17641 cgggataaac atgtacacct gaaaacaata tggtattttc gcctaccgaa gaattgtctt
    17701 cgatattgct atgagaatga atttgcgtgt tttttcctat ttttacattt tcacccacat
    17761 aaacaaaggg agcaatgtaa acatcttcac caatttgggc agtagaagct atgaaagcca
    17821 atggagaaat gccctttttt ttgggtttgc tctgttcgac cagcgaaaaa agctgcgcca
    17881 aacattcata cgcattgttg acacgaataa gcgtcgctgg aatttctttc tcaggcttga
    17941 aatccttgtt taccaataca atgcttgcat tacaatcgta aatataatga ctgtattttg
    18001 gattggacaa aaatgttatg gttcctggtt ttccttcctc tattttagaa aaattactta
    18061 cctttactga tgggtctcct tccacctggc cacctaaaaa atctgcaatt tgctgagctg
    18121 taaactccat agtttttaaa aattttgggt acaaaagtac gaaagaattt ttaattattc
    18181 actttgtaac acttgttatt tttattctac aattttattt tacagaaaaa tatcttgctg
    18241 tcggctaaat tcggcaataa cagaaataat tcttgtaaac tttcttggtc aaaacattta
    18301 tattaagcat atcggacgcg tcggcaatat ctttcaccgt tccatctttg tatagaatgt
    18361 taattttctc atcaacaggg tcatacgtat ctgtgctgac gatttcctct cccatgaaat
    18421 attcggcttc ctgctgcgaa acatcaaaat accggctgta ttttttcaat aattcctctt
    18481 tttttgtttc gtccaaaggc tcctgcagca tttctacctt aaacaatttt ctgttgataa
    18541 aagcattgca aagaaaagaa agaactttat cgggatggtg agcccaaact ttgatggcac
    18601 aaatcacatc cgaatcatca agctgaacaa aataatccaa tgcttcggtt gaacgaacaa
    18661 aattctgctt gtccagatga tgatagagaa aataatgcaa agcaggagaa gcaaataatg
    18721 tttcaccaac ggaagcaagt tccttggcac gcttgagaat atttgtcaac attttttcgg
    18781 tagcaaccag cgttttgtgc aaatacacct gccagtacat caaacgacgt gccaccagaa
    18841 atttttcaat ggaataaatt cctttcaact caacgaccaa ttctccgtga aaaacattca
    18901 acattttgat aatacgcgcc gaaccaatga taccttcact gactccgcaa aaaaagctgt
    18961 ctctgctcaa gtaatccatt cgatccatgt cgagttgtcc cgaaaccaac tgatgaagaa
    19021 attttttagg ataatggcca ctaaatattt ccagcgccaa agccaaatta ttattcattt
    19081 ctttattcaa ttgctgcatg agcattattg aaatttcttc atggtgaatt ccttttacca
    19141 agctgttttc caaagcatga gaaaacgggc cgtgtccaat gtcgtgcagc aacatgcacg
    19201 cccgggcagc ctgctcttct tcgccggaaa tcacatgacc tttctgacgc aactcttcaa
    19261 tggcttcacc cattaagtgc atggccccca acgaatgttg caaacgggta tgttgcgctc
    19321 ccggataaac atagaacgaa acacccaatt gcttgatgcg gtttaatctt tgaaaataag
    19381 gatgctgaat aatttcatat aaaaaatcat ccggtatgtt gataaaacca aaaaccggat
    19441 cattgataat tttgagtttg ttgggttgta tccgactttc tccgagcgag ttgtttcccg
    19501 agatatttgt catttcagct tttttcgaga attttaagca accattgcca acatttccgg
    19561 gtagaagaga tacttaaccg ctcattggga gaatgaactc catacatttg cggtccaatg
    19621 gaaatcatgt cgagataagg atattttttc agaaaaagtc cgcattccag tccggcatga
    19681 atagcccgaa ctttcggttc ttcacacgtg atatttttgt aacaaacttt ggctttctgc
    19741 aatatttcgg aattcaaatt gggtttccag cccggatatc cttcaccatg cgttgcttga
    19801 gcttgtgcaa gtgtaaagac agattcaacc tgatgggcaa tgtcgaactt ggccgattca
    19861 acggaactgc gctggctggt tatgataaga aaagaattat tttcctgcat tcggacagaa
    19921 gccagattgg tggaagtttc caccagattg ggcatatctc tgctaaaagc ctttactcca
    19981 tgtggacagg cataaagtgc atttaaaagc ttatcggaag atttcttgtc gagtacctga
    20041 tcgggaattt cttctgaaga aataaggatt tgcaaagccg gttcatcttc tttgagttca
    20101 ccctctattt ccgcaataaa ttcgttgagt aacacacgaa gtttttcctt atcctcaaaa
    20161 ggtacggaag ctacgacttc ggcttcgcgt ggaatggcat tgtgcaaatt tcctccccga
    20221 aaagtatgga ttctcaaatc aagttttttg tttacattcc acagaaaacg attcagaatt
    20281 tttatggcat ttccgtgtct tttgtcaatg tcgtcgccgg aatgtccacc tttcaatcct
    20341 ttcacggtca gcgagaagga aaagtaatta ttcggagatt tttcagctat aaaaggaaaa
    20401 tttattgtcg tatcgatacc tccggcacaa ccgataaaaa taacaccatc atcttccgaa
    20461 tccagattga taagaatttg tccgctgagg aaatgatctt caatagccga agctccggtc
    20521 agtcctgttt cttcatcaac agtaaaaagg cactcaagcg ccggatgtga aatgttgtcg
    20581 gaagtaagca cagcaagcat catagccatg cctatgccgt tgtcggctcc taaagttgta
    20641 ccttccgcct ttatccaatc accatcaata aacgtttgaa tggggtcgga gtcaaaattg
    20701 tgctgcacat cgccatcttt ttcacaaacc atatccatgt gcgactgaag aatgattttt
    20761 ttgtgatttt cgtaaccgaa tgttgccggt ttacaaatca gcacattccc tgcctggtct
    20821 gtttttgcct caagtcggta ttgttgggca acatccagca aatattccct tattttttct
    20881 tctttttttg aaggtcgggg aatttgggtg atttccccga aaaatttcca aacaactgaa
    20941 ggctcgagat tttccataaa ttgattgttg tacgttgagg aaaattaaac tcacaaagat
    21001 atttaaaatt tttgtaacaa cagaaagacc gacagtaatt ttgaattttt atgcaggttt
    21061 ccaaagctta tggaagaagg gtctgattat ttcaactcag ctgctccgcg aatttcggcc
    21121 gaaacttcgt tcacccagcc ggcacgctga aaaacagccg tttgaatttt gataaagcga
    21181 attttttcca gataaacagc cttacctgaa gaatcgacgg cattggaaat gtccagttta
    21241 ttggaatgat gatgggtatc ataatcggtt ccaaaattat tttcggcata accccatata
    21301 aaacgatcag ttggaacaat ccaactttgt gcactttcag aagatttgtt gttataagca
    21361 tccggcaaac gtgaaccttc aaaagtaata ctgtcttcca tacaggttgc ccaccaccag
    21421 gaggaagtgt tgggagctcc aaatcccgaa atcaattctc cgttctctcc ttcactgtct
    21481 ttccactgaa tgttgtcatt ggatgtcgct ggcttgtagt aacgaaccca ataatttctt
    21541 ttttcatttt caaactgatt tccctttaac tcataccagg tatcgttggg cttaccatca
    21601 ccattgtcgt cgggcattac ataaacaatt gccggttccg gcgaagctcc gggcaaagca
    21661 aatatttcca aatcgtatcc atctttgttt tgaatatcgt ggtcgaaccc ggcaattaca
    21721 tatccgccaa aaccgcccaa atacaaaaat cccttttcat ccgaaggctc accaataaaa
    21781 tgctcgatat cggcaggttt tgccagttgg gcatgttggc cgggtgcata aacatattca
    21841 aaaacctgcg taacataaat agaattggta atatccggct cattttcttc cttttcgcca
    21901 cacgagaaaa acgacactga taaaataaga ctgaaaacaa aaagaaaata ccgtttaata
    21961 aaaaatttgt ctatcatgct aaatttgttt aagtaaatat tttttaatga aatgaagatt
    22021 tgaatttgga gttaggagtt tttagtaaag agttaggagt aaagagttag gagttaagtg
    22081 ttttattaat aaattgatta taagtctgtt aaactttatt gttttgaaaa cagacagaaa
    22141 atctgttatt taaaaaaatc aatggttttg agaagaaagt tgaaacctga taatgattgg
    22201 acagatcgaa attttaaata ttccgataac ctaaaacaaa agtatcgaat gattaacgga
    22261 tagtaaccat tgtggctcca aagccatatt gttgaaagga agcatcttga aaataataac
    22321 ttttatactt ggtacgaagc aatttttcaa tttcctttcg cagcactcct tctccttttc
    22381 cgtgtatgaa gacaattttt tgtccttttc gatttttgta atcattcaaa acggattcaa
    22441 atttttccag ttggagttcc agcatatcgg cattattcat accgaccgta gtatccacaa
    22501 gctgattgat atgcagatcg acttcaacaa tttccggatt ttttgcaggt ttgctgatgc
    22561 gtggtcgccg ttcgggcttt tccttttggg aaatggcttg tttaatttct tccgctgaaa
    22621 tgtcgtgcaa ttgatgcgtt tcaatttctt ccaaaggatt caacatcatg gcaggcacat
    22681 cgaaatactc attttcaccg aaactgtgaa gcttgtaaaa cttcatcagg ttcaatttga
    22741 gatcgatatc aataaccggt tgcggccgat aagttttatg ttttttaaag ggtatgattt
    22801 gaactcgaat cttttcccaa gcattcagct cttctttttt aatctctgcc atccattcct
    22861 gcatattggg ttcaatcaac ccgttggcca cacttttgca gcattcgttt tcaaacaatg
    22921 caaggttgta aaacagaaaa taattgctgt cgttcaccaa ataaaagtcg ttcgaggtgg
    22981 tctgcaactg cttgatatct tcaggcacaa aagccagaaa aactctcagg ttttctcctt
    23041 caggcgtttc aaagatttct tcctctataa tttcaggttt tggtggtaag ttgatggact
    23101 tagcggccgg cgattcgttc tcattcataa aagctgctga agccgattta aaatcctttt
    23161 tgggaaaatt ggtattttca ttcacttccg gaatgaccac acattcacgt tccagcattg
    23221 gaatttcaaa tccgtcagca tcggaaacgt acacgatttt ttccctttca tctattctgg
    23281 taacaattcc cccaccagta gaattgagaa aacgtacttt atctcctact tttatcatgt
    23341 tgttcggttt ttcaaaaaaa cattcttaca tctttatctt ccaaactgct tttgtgcagt
    23401 gagatacgaa cggatcggaa attttttcga tttttcgtat ctttgcacaa aattacaaca
    23461 atatttttgt atctcccgtg aacgaattaa aagaaaaaac gaacttttcc attgatgatt
    23521 tcgattacgt cttacccgaa gaacgcattg ccaagtatcc gttaaaaaag cgcgatgctt
    23581 cgaagttgct gatttacaga gatggaacaa tcaccgaaaa tgtttttagc aatattgctt
    23641 cttttttgcc cgagaaggca ttgctggttt acaacaatac aaaagttatc caagcccgta
    23701 ttcatttcag gaaagaaact ggcgcgttga tagaagtctt ttgcctggag ccagctcagc
    23761 cggccgatta cgcattatct ctctcggcaa ccaaagaatg tgtttggaaa tgttttgtcg
    23821 gaaatcaaaa aaaatggaaa aacggcactt tgtccaaaga actcgacata aacggaacgc
    23881 aatttgagtt ctccgccgaa ttgctcgaaa gaaaggggaa tgtaaatttt gttcgtttta
    23941 gctggaacaa tgaaaatatt cactttgccg aaatcctgga aaaggcaggc gaattgccta
    24001 ttcctcccta cttgcacagg cctacggaag aaagcgattt gataacctat caaacggttt
    24061 atgcaaaaat caaaggttcg gtggctgctc ctacggcagg attgcatttt accccggaag
    24121 tttttgaaag tttgaaatcc cgcaatatag agactgccga attgacttta catgttggcg
    24181 ccggaacttt tcaaccggta aaaacaaaac aaatttccga tcaccaaatg cacacggaag
    24241 tcattgaaat tcaaaaaaaa acaatagaaa agctgttaaa aaatatcgga aaaatcattg
    24301 ccgtgggaac cacttccgta cgaacactgg aaagtttgta ccacattggt ttacttatcg
    24361 agaagcaaca atttcccgac gaaaagcatc cttacttttt tatcccgcaa tggatgcctt
    24421 accagactga atgtgttctg actccggaag catcattgca attaatattg aacgaaatgg
    24481 agaaaagaaa tctctccgta cttcatgctc aaacgcaact gattattcaa cccggttaca
    24541 cattccgact gaccaacgga atgattacca actttcacca accaaaaagt actttgctac
    24601 tgctggtttc tgcctttgtt gatggcaact ggcgaaaaat ttacgattat gccctttctc
    24661 acgattttcg ttttctgagc tacggagaca gttctctgtt aatgaaatag ttttttgtaa
    24721 ttttcaatag caataatcat ttttagagaa tatgaaaccc acatgtatta catacacaag
    24781 cacgaatgaa tataatattc agacatgtgg gttccatcag acc
//
LOCUS       DAOTAX010000028        22949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1848_length_22949_cov_32.269503, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000028 DAOTAX010000000
VERSION     DAOTAX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22949
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1848_length_22949_cov_32.269503"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            663..1754
                     /locus_tag="PLM04_04585"
     CDS             663..1754
                     /locus_tag="PLM04_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HON02385.1"
                     /translation="MYIESINIVNYKNIRQAELSFSPKINCFIGNNGMGKTNLLDAVY
                     FLSFCKSHSNFIDSQNIFHEADFAVLQAKYRMGEATEEIYCGMKRNQKKQFKRNKKDY
                     ERLSDHIGLLPLVLISPEDSVLISEGSDERRKFVDGVISQYDKKYLHNLLLYNNALKQ
                     RNALLKVETPVDESLFEILEEQMADYGSYIFEQRTRFIDQLIPVFQQYYRYISSHENE
                     EIDITYLSHFQQNDIKKQMKSTRERDKMLGFSTRGIHKDELEMLLNGYPIKRVGSQGQ
                     NKTFLVSLKLAQFKFLKQTHHQPPILLLDDIFDKLDADRVRKIVELVSQETFGQIFIT
                     DTNREHLDQILYQLDQEAKIFKVENGIIL"
     gene            2054..4603
                     /locus_tag="PLM04_04590"
     CDS             2054..4603
                     /locus_tag="PLM04_04590"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HON02386.1"
                     /translation="MKPKTYTAEEVKKASFEYFQGDELAATVWSTKYALKDSYGNFYE
                     LTPDDMHKRLAREIARIEKHYPNPLTEDELFELFRHFKYIVPQGSPMTGIGNDFQIAS
                     LSNCFVIGFDHDADSYGGIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLTVSIKHPDSESFIDAKLEAGKITGANISVKI
                     HDDFMQAALAQETYTQQFPIEDDHPWFKKEIDAHSLWKKIIHNAWQSAEPGVLFWDTI
                     TRESVPDCYADLGFKTISTNPCGEIPLCGYDSCRLLAINLYSYVNHPFTPEASFDFDL
                     FKKHVAVAQRIMDDIIDLEMEKIDKIMAKINADPEDEEIKRTELKLWEKIKNKTSMGR
                     RTGVGTTAEGDMLAALGLRYGTNSAIDFSEQVHKILALAAYRSSVTLAKERGAFSIYD
                     AKREEKNPYILRLREADPELYDEMVKHGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVRVDFVDENGDSWEEYVVFHHKFVTWMEANHYSTTQKYTKAEL
                     DELVEKSPYYKATANDIDWLKKVQMQGRIQKWVDHSISVTINLPADTTEELVGQLYEE
                     AWRCGCKGVTVYREGSRAGVLLSVEEKKSEKSGERKICFPQSEEHLIRPKELDCDVVR
                     FQNAKDKWIAFVGLKDGRPYEIFTGLADDEEGIALPKTVTQGKIIKTVDENGNKRYDF
                     QFTNKRGYKTTIEGLSHKFDKEFWNYAKLISGVLRYGMPIDQVIKLVSGLQLDSESIN
                     TWKVGVERALKKYIPDGTEANGKNCPSCGQKTLIYQEGCLTCKNCGYSHCG"
     gene            complement(4904..5497)
                     /gene="folE"
                     /locus_tag="PLM04_04595"
     CDS             complement(4904..5497)
                     /gene="folE"
                     /locus_tag="PLM04_04595"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HON02387.1"
                     /translation="MDFDITSSRPFDEKKTGEIAQHYQEIIRLLGENPDRQGLLQTPQ
                     RVARAMQFFLQGYEQDAEEIIRTATFTENYRQMVIVKDIDFYSLCEHHLLPFFGKAHV
                     AYIPNKKITGLSKIARVVEIYARRLQVQERMTTQIKQCIQNTLDPMGVMVIIEAQHLC
                     MQMRGIQKQNSITTTSDFTGVFQEARTREEFMNLINR"
     gene            complement(5521..5997)
                     /locus_tag="PLM04_04600"
     CDS             complement(5521..5997)
                     /locus_tag="PLM04_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HON02388.1"
                     /translation="MMKNFKFLLFTIFLAVSGGLQAQDESTSVRQNIFEVLQASDGAG
                     KVIIHQDKRIENLINQKQTAKTGREATLGNGYRVQVFSSNEQRTGKVEAFKIEKEIRE
                     AFPNTGVYVNYNSPFWKVRVGDFSSVDEAQAFRNKLLEVFPDLRSQTYVVRDQIVK"
     gene            6152..6886
                     /locus_tag="PLM04_04605"
     CDS             6152..6886
                     /locus_tag="PLM04_04605"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HON02389.1"
                     /translation="MELTKIVLIVAGGKGERMQSDIPKQFIEIAGKPILMHTIEAFAR
                     YDFDLPVFVVLPVTQIEFWKNLCSKYSFKFSHQVVIGGETRFHSVKNGLEAVAAFLAS
                     LYPDKSEEENNSALVAVHDGVRPLVSVETIRRCFDEAEKFGSAIPSMDLVDSLRTIHT
                     KYSQSLDRSAYKLVQTPQVFDFNILKKAYEQEFSPLFTDDASVVEAIGVNVHLTEGNR
                     ENIKLTTAFDLELASFLLAKRENSSY"
     gene            6919..9015
                     /gene="recG"
                     /locus_tag="PLM04_04610"
     CDS             6919..9015
                     /gene="recG"
                     /locus_tag="PLM04_04610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HON02390.1"
                     /translation="MDFFTQDIQFIPGVGPKRAELFKKELNIRTAEDLLRHYPYKYVD
                     RSRFYMISEISENMPFIQLKGKILKFEKVGEGCSQRLIGVFTDGRQYIELVWFKGLKF
                     VAERYKTNIEYVVFGKPTLFNGKYNIVHPDIEILSQLPPVEQMGLQPFYNTSEKMKTH
                     FLNSKAIQKIIYSLLPTLQNGIPETLPAYILNRYGLLNLKDALVNIHFPKNSHLLNRA
                     RQRLKFEELFYIQLDIVKQTAWRNQRFKGFVFKHVGYYFNTFYRDYLPFELTNAQKKV
                     IREIRSDVASGKQMNRLLQGDVGSGKTLVALFAMLMAVDNGFQACMMAPTEILAHQHF
                     ESLKQFLGNIGIQIALLTGSTRPKERAAIHEKLLSGELNILLGTHALIEETVQFKNLG
                     LVVIDEQHRFGVAQRARLWSKNVQPPHILVMTATPIPRTLAMTIYGDLTVSVIDELPP
                     GRKPVQTFHFYDNKRQALYKFMVKQIEAGRQIYVVYPLIQESETMDLKNLEEGYQHLS
                     EIFPQYQISMVHGKMKPAEKEAEMQKFVAGKTQIMVATTVIEVGVNVPNASVMIIENA
                     ERFGLAQLHQLRGRVGRGADQSYCILLTGYKLSNLTRKRIETMVNTNDGFQIAETDLQ
                     LRGPGDLDGTQQSGLPFELKIANLAQDGKMLEMARKVAMDIIEEDPQLQKKENLILSE
                     QLKKMKTSSLNWSVIS"
     gene            9116..9577
                     /gene="ndk"
                     /locus_tag="PLM04_04615"
     CDS             9116..9577
                     /gene="ndk"
                     /locus_tag="PLM04_04615"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HON02391.1"
                     /translation="MEKTLVIIKPGAIQRELIGEIITRFERKGLHLCGIKMYQFTDKV
                     VSEHYAHLADRPFFKRIKDSMMVTPVIVCCWEGLDAVRIVRSMSGVTNGREAAPGTIR
                     GDFSMSVQENIVHASDTVENAKVEIDRFFKPEEIFEFKPNNFPFVYANDEV"
     gene            9750..10487
                     /locus_tag="PLM04_04620"
     CDS             9750..10487
                     /locus_tag="PLM04_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HON02392.1"
                     /translation="MLNDTSDDLMENHPADDIYNNIWTSERLNPYHFPVDSLPDSVRI
                     DCSNFILPIYGKVTSKFGRRRYRYHYGIDLKLNVGDSVRCAFEGKVRIIDYEHGGYGH
                     YVVVRHNNGLETVYAHLSKVLVELNQIIGAGEVIALGGNTGRSTGPHLHFEMRYLGNA
                     IDPDNLVDFEKGSIRQFSYLITKKNTFKYQKDLKSLQMARYHVVHKGDTLSLIASRYG
                     TTVKQLCNLNQISSKKLLKPGQKIRVR"
     gene            complement(10927..11790)
                     /locus_tag="PLM04_04625"
     CDS             complement(10927..11790)
                     /locus_tag="PLM04_04625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON02393.1"
                     /translation="MKKLNLFLLVAISATFTFSSCNSDKPGKPTLKSQIDSLNYAFGV
                     ANGDGIKNYYLQGDSTGKALNSLLKGINDGLKSKVDKENMELASIGSQIGYSLKEQSK
                     SGLMGDSSLSVNINLVKQGLINGMKGSNIQMTPQEASEYLQSTMQSLQEKKIEKQYSA
                     NKEAGEKFLLENAKKSGVITTESGLQYEIIKKGKGPIPTDSSKVKVHYHGTLIDGTVF
                     DSSVERNQPAEFYVNQVIKGWTEALKLMPVGSKFKIYVPYQLAYGSANQGAIKPFSML
                     IFEVELLAIEK"
     gene            complement(11840..12367)
                     /locus_tag="PLM04_04630"
     CDS             complement(11840..12367)
                     /locus_tag="PLM04_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HON02394.1"
                     /translation="MKKQIGFMLATVLAVFLLASCSKKYEAKKVQLKTQNDSLNYTLG
                     LANGEGIKNYYLQNDSSDAPIKALINALDKAYSSKEVDDQMYQLGYQIGTSLKYQKEN
                     GLMGDSTLVFNTKLFKLGLVNGLHGFDGGMTPQQAQEYLQKTMTELQAKKSAQQAPAN
                     QPQPAPESQKTDSVQ"
     gene            complement(12408..13130)
                     /locus_tag="PLM04_04635"
     CDS             complement(12408..13130)
                     /locus_tag="PLM04_04635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON02395.1"
                     /translation="MRKIFVLSLLAIFLITSAEAKKPKKIQEEPPVQKLTNTIDSMSY
                     ALGLSIGSDFIKNLKTIPGGQFNIDWLIQGFTGGMKKDSTVMTTAFANNYFREYITKA
                     QSLESAKKKEVADAFMEKNKTRESVITTESGLQYEILACANGPKPSINDTVEVNYQGF
                     LPDSTKFDSTFDRGEPAVFALNQVIPGWSEGLQLMTVGSKYKFYIPYELAYGEQGAGG
                     VIPPYSPLIFEVELLQINPAKK"
     gene            complement(13228..15009)
                     /locus_tag="PLM04_04640"
     CDS             complement(13228..15009)
                     /locus_tag="PLM04_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HON02396.1"
                     /translation="MKKTCLIFMYLLPVFSFGQVLDDFSDGNFTENPSWSGTTGNFKI
                     NEYFQLQSNAPTTSVSYLFTPSEVFYDAEWQAWFKINYSTSSSNYSAFYIISSSPDNL
                     GNAYFVKIGDTQDDVSLWRQQGTSKIKIIDGTDKRTDGTQVEIRVKVTRDSLGYFSLY
                     SKLPSESDFVLEGTVRDTTIESSSYIGLLYSNTTTTTSAYFFDDIAANGKKAVDKKPP
                     VWEKLAIIEPDTLQLQFSEPVDLSNSIFILNENEITPTKIDILSTKPFLMNVALNENF
                     DKGKIYTLELLQVTDLAGNTLLDNIKKTGIIEPLAQGDLLLNEVMFNAPPTATEYIEI
                     YNASAKTLDVSGIVITTRKSDGSLNTLRHIPDGKLLAAGGYLAVCQHADSVMNYFGLP
                     NDGSIVSISWTALNNQQSTIVLANSKADTVYDELTYNEKWHHLMIKNRQGVALERINP
                     ALPTQSAESWHSAASEVKYGTPGYKNSQYREIENTFSNKQFVWVEPEAFSPDNDGTDD
                     VCFIKYRTPTEGFVANAQIINATGTKVFQLVSNALLSSEGFFSWDGRTDSGKIANAGI
                     YVLYFEMIQPKTGQKKQMKIPIVVSAR"
     gene            15175..16182
                     /locus_tag="PLM04_04645"
     CDS             15175..16182
                     /locus_tag="PLM04_04645"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HON02397.1"
                     /translation="MRVAIVGASGAVGQEFLRVLAERNFPLTSLSLFGSSRSAGSVYD
                     FKGEKLTVKELKHNDDFKDIDLAFTSAGAGISKEFAEDITKYGALMIDNSSAFRMEND
                     VPLVVPEVNASDAKNRPRGIIANPNCTTIQMVVALKAIENVSHIKRVHVATYQAASGA
                     GAAAMAELELQYRQVLAGETPTVNKFPYQLAFNVIPQIDVFTENGYTKEEMKMFNETR
                     KIMHSDVVVSATCVRVPSLRAHSESIWAETERPLSLEEVKAAFENAEGVVVMDNPAEK
                     LYPMPLFLSGKDPVYVGRIRKDLANPNGITFWCVSDQIKKGAALNAVQIAEYLIKEKE
                     F"
     gene            16465..17901
                     /gene="sufB"
                     /locus_tag="PLM04_04650"
     CDS             16465..17901
                     /gene="sufB"
                     /locus_tag="PLM04_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HON02398.1"
                     /translation="MSEDIINQVTQGEYKYGFTTDIDTEVFPMGLNEEIIRLISAKKN
                     EPEWLLDFRIKSYRHWLTMKMPTWAHLEIPPIDFQKISYFAAPRKKSPKSMDEVDPEI
                     LKTFDKLGIPLEEQKALSGMAVDAVMDSISVKTTFKENLAELGIIFCSFSEAVQHHPD
                     LVQKYLGSVVPYSDNFFATLNSAVFSDGSFVYIPKGVRCPMELSTYFRINASNTGQFE
                     RTLIVADEDSYVSYLEGCTAPMRDENQLHAAIVEIVALKNAEVKYSTVQNWYPGDKEG
                     KGGIYNFVTKRGICKGENSKISWTQVETGSAITWKYPSCILQGDNSSAEFYSVAVTNH
                     FQQADTGTKMIHLGKNTRSHILSKGISAGFGQNSYRGLVRVSKNADNARNFSQCDSLL
                     LGDKCGAHTFPYMDVNNDTAIVEHEATTSKISEDQIFYCNQRGISTEDAVGLIVNGYA
                     REVINKLPMEFAVEAQKLLQITLEGSVG"
     gene            17957..18721
                     /gene="sufC"
                     /locus_tag="PLM04_04655"
     CDS             17957..18721
                     /gene="sufC"
                     /locus_tag="PLM04_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HON02399.1"
                     /translation="MLEIKDLHASINGKEILKGVNLTIRAGEIHAIMGPNGAGKSTLA
                     WVLSGNPAVEVTHGEIIFNGKNLLEMSPEDRSREGIFLSFQYPVEIPGVSMVNFMRTA
                     VNEQRKYKNLEPLSAADFLRLMREKKELVEMDNKLTSRSVNEGFSGGEKKRNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGVNKLKRPDNATLVITHYQRLLDYIIPDFVHV
                     FYDGRIVKTGDKNLALELEEKGYDWIKKEVETPVEQ"
     gene            18756..20093
                     /gene="sufD"
                     /locus_tag="PLM04_04660"
     CDS             18756..20093
                     /gene="sufD"
                     /locus_tag="PLM04_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HON02400.1"
                     /translation="MEQQYIDFYKESHTLIKQHSAALMNNFRDAAFEKFERLGFPTSK
                     LENYKYTDMSAPLSIEYGLNINRLQFQINPYDVFKCDVPAIHSYLFFVLNDTFYPVNS
                     TAKQHLPEGVIIESLRTAAENYPQLVEKYLGKLSNEKNDGWIAFNETFAQDGFFMYVP
                     KNVKLSKPVQLINIMRSDVDFMANSHNLIILEEGAKAQLLVCDHTMDNVHFFSNRLTE
                     VFAGKNAVYEHYKLENTNILNTNVSNLLIHQEDSSNVLTNVITLHNGMTRNGIEMIID
                     GENCESLLCGMVIGDKNQDVDNFTEIVHKKPNSQSRELFKYVLDDSSKGAFAGKIQVA
                     KNAQKTSSFQTNKNILLSKTAKMRTKPQLEIYADDVKCSHGATIGQLDEAAKFYMQSR
                     GISESEARLLLMFAFTADVMDNIRIDALRDRMKLLVEKRLRGELSRCEGCMIC"
     gene            complement(20308..21609)
                     /locus_tag="PLM04_04665"
     CDS             complement(20308..21609)
                     /locus_tag="PLM04_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HON02401.1"
                     /translation="MNINNLKFETLQVHAGQEVDKTTKSRAVPIYQTSSYVFDDAADG
                     ADLFALRKFGNIYTRLQNPTTDVFEKRMAALEGGISALATSSGQAAEFLALNNIVQAG
                     DNFVATSHLYGGTYNQFKNQFKRLGVDVKFTANDEPETFEKLIDDKTKAIYIETIGNP
                     DLNIPDFEAIAAVADKYDIPLIVDNTFGAGGYLFRPIEHGASIVVESATKWIGGHGTS
                     LGGVIVDSGKFNWGNGKFPAFTEPSESYHGLVFWDAFGADSPFGNIAFNIRARVEGLR
                     DWGCVISPFNSFLLLQGLETLSLRMERHVQNALELARWLQTLSAVEYVNYPGLENNPY
                     HEMAKKYLKKGFGGVLAFKLKGDASMADKFINNLKLISHLANVGDAKSLIIHPATTTH
                     EQLSPEDKIAAGVFPGLLRFSVGIENIDDIKADIEQALSNI"
     gene            complement(21643..22590)
                     /gene="cysK"
                     /locus_tag="PLM04_04670"
     CDS             complement(21643..22590)
                     /gene="cysK"
                     /locus_tag="PLM04_04670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HON02402.1"
                     /translation="MSKIAKNLTELIGNTPLLQLTHFVSNKGLQANIMAKLESFNPAG
                     SVKDRIALAMIEDAEEKGLLKQGSEIIEPTSGNTGIGLAMIATVKGYKLTLTMPETMS
                     VERRNLLKALGANIVLTPGTEGMKGAIAKAHELHDQNPSSFMPQQFENPANPLKHFHT
                     TGEEIWRDTEGKVDIFVAGVGTGGTISGVGAALKKHNPAIKIVAVEPASSPVLSGGKP
                     GPHQIQGIGAGFVPKNFDPTVVDEIIQVSNDDAIRTSRQLAQQEGLLVGISSGAAAFA
                     ASLLSQRPENKGKNIVVLLPDTGERYLSTALYAFDEYPL"
BASE COUNT         7051 a         4317 c         4538 g         7043 t
ORIGIN      
        1 ccgtacatat tttggatgtg gaaagctccg tccgcgatat tgtcaatatg actgctatcg
       61 ctgttcttga tgccattgta gaagaaaaaa ttacaaccga aaatctcgaa cgtatttaat
      121 aattttttct ttctaaacaa ttgctcgatc ataggaaaaa tggtcgagca tttttaatta
      181 ttttcaatcg ttttgccgct catcattcat aatttccatt tccaaacagg cttgcagaat
      241 ttttagattt aaattataca caaaccaata atttctgcag cctccttatt ctctgatggt
      301 atcctttcta tttcgttcgg tttggtctaa ccaattacct gtttgcgaat caaaagtttt
      361 ttatgcgata aatacttttg gcctccggat gcgttttgaa atgattcata ataaacattg
      421 atgcaaaatt gctttttacc aaaaagggat agcatggttt tgatgaatat gggacagctc
      481 ttctgagctt gactttcctt ttttaaattg tcaaagctta atcctggttt aatcccctga
      541 atttcgaaat ttcatgctaa agaagccgac gtcagcgtta ttcatgtatt tttgtttttt
      601 accgtgtgaa attcaaaaat tatcccgtaa atttgtgaac acaaagtttt gatttagcat
      661 tcatgtacat cgaatcgatt aacattgtca attataaaaa catccgtcag gctgaattga
      721 gcttttcccc gaaaatcaat tgctttattg gcaacaacgg gatggggaaa accaatttgc
      781 tggatgccgt atattttttg tctttttgta aaagtcattc caactttatt gattcacaaa
      841 atatttttca tgaggccgat tttgccgtat tgcaggcaaa atatcgtatg ggcgaagcaa
      901 cagaggagat ttattgtgga atgaaacgca atcagaaaaa gcaatttaaa cgcaacaaaa
      961 aggactacga acgtctatcc gaccacattg gattgttacc gctggtattg atttcgcccg
     1021 aagattcggt ccttatttcc gaaggcagcg acgagagaag aaagtttgtt gatggcgtga
     1081 tttcgcaata cgacaaaaaa tatctgcata atttattgct ttacaacaat gcgctgaagc
     1141 agcgaaatgc cttgttgaag gtcgaaacgc ccgtcgacga atctttgttt gaaattctcg
     1201 aagaacaaat ggccgactat ggttcttaca tttttgaaca acgaacccgt tttatcgatc
     1261 agttgattcc tgtgtttcaa cagtactatc gctatatttc ttcccatgaa aatgaggaga
     1321 ttgacatcac ttatttgtcg cattttcagc aaaacgatat aaaaaaacaa atgaagtcaa
     1381 cccgtgaacg ggataaaatg cttggttttt ccacacgagg cattcataag gatgagttgg
     1441 aaatgttgtt gaatggttat ccgattaagc gtgtgggttc acaaggacaa aataaaactt
     1501 ttttggtttc gttgaaattg gctcaattca aatttcttaa acaaacccat catcaaccac
     1561 cgatactttt attggatgat atttttgaca aactcgatgc cgatcgtgtt agaaaaattg
     1621 ttgaacttgt ttcacaggaa actttcggtc agatttttat taccgacacc aatcgcgaac
     1681 atttggatca aattctttac caactcgatc aggaagccaa aatttttaag gtagagaatg
     1741 gaataatttt gtaagtttta ttaatctcat ctatcttttt tctatttata ttattaggtt
     1801 atagatttcc tttattgaaa attaattata tgcaaaagtt ttcaaataag gaaatttttt
     1861 atttaatata aaaagttaaa ttattaataa tcaataatat aatttttgaa atagaaaatt
     1921 tttaccgaaa aggaaaaata attattgaaa ttattggata atcatttttt tatttataaa
     1981 cttgcagttg taaaaaatgc gtcgctaatt tttcttaaac ctttatttat taacccaatt
     2041 aaaaattttt gttgtgaaac caaaaactta tactgccgaa gaagtaaaaa aagccagttt
     2101 tgaatatttt cagggagatg aattggctgc cactgtttgg tcaacaaaat atgcgttaaa
     2161 agattcttac gggaattttt acgagttgac gccggatgac atgcacaaaa gattggctcg
     2221 tgaaattgcc agaatcgaga aacattatcc caatccgctg acagaagatg aactttttga
     2281 acttttccgg catttcaagt atatcgttcc acaaggaagc cccatgaccg gaattggaaa
     2341 cgattttcaa atagcttcac tttccaattg ttttgtcatt ggttttgacc atgatgccga
     2401 ttcttatggc ggtatcatta aaattgatga agaacaggtt caattgatga agcgtcgggg
     2461 tggagtgggg catgatcttt ctcacattcg ccccaaaggt tcgccggtga aaaattccgc
     2521 gcttacttct acgggaattg taccttttat ggagcgttat tccaattcta ctcgtgaagt
     2581 tgcacaggat ggtcgtcgtg gtgctttgat gttgacggtt tccattaaac atcccgattc
     2641 ggaatcgttt atcgatgcaa aattggaagc agggaaaatt acgggtgcca atatttcggt
     2701 gaaaattcac gatgatttta tgcaggctgc tttggcgcag gaaacttata ctcagcaatt
     2761 tcctatagag gatgaccatc cgtggttcaa aaaagaaata gatgcccatt ccctgtggaa
     2821 aaaaattatc cacaatgctt ggcaatcggc tgaaccgggc gtacttttct gggataccat
     2881 tacccgtgaa tctgttcccg actgttatgc cgatttgggt ttcaaaacta tttctaccaa
     2941 tccttgtggt gaaattccac tgtgcggata cgatagctgc cgactgctgg ctattaatct
     3001 gtattcgtat gtcaatcatc catttacgcc ggaagcttca ttcgatttcg atttgtttaa
     3061 gaagcatgtc gcagtagctc agcgaattat ggacgatatt attgatctgg aaatggagaa
     3121 aatcgataaa attatggcca aaataaatgc cgatcccgaa gatgaagaaa tcaaacgcac
     3181 ggagctcaaa ctttgggaaa aaattaaaaa taaaacttct atgggtcggc gtaccggtgt
     3241 gggtaccaca gctgaaggag acatgttggc tgcgttgggt ttacgttatg gaaccaattc
     3301 tgccatagat ttttctgaac aggtacacaa aattcttgct ttggcggctt atcgttcttc
     3361 tgtgactctt gccaaggaac gcggtgcttt cagcatttat gatgcaaaac gcgaagaaaa
     3421 aaatccctat attctccgtt tacgtgaagc cgatcctgaa ctgtacgatg aaatggtaaa
     3481 acatggaaga agaaacattg cctgccttac cattgctcca accggaacaa ccagcatcat
     3541 gacacagaca acttcaggta tcgaaccggt ttttatgccg gtttataccc gtcgtcgaaa
     3601 agtgaatccc aatgataaaa atgttcgcgt agattttgtg gacgaaaatg gcgattcatg
     3661 ggaagaatat gtggtttttc atcataagtt tgttacatgg atggaagcca atcattattc
     3721 cactacccag aagtatacca aagcagaact tgacgaattg gtggaaaaat ctccctatta
     3781 caaagcaaca gccaacgata ttgactggtt gaaaaaagtt cagatgcagg gacgaattca
     3841 aaaatgggtg gaccattcca ttagcgtaac catcaattta cctgccgata caaccgaaga
     3901 gctggttggt caactttatg aagaagcatg gcgttgcgga tgtaagggag taaccgttta
     3961 tcgtgaaggt tcacgagccg gcgttttgct gtcggtagaa gaaaaaaaat ccgagaaatc
     4021 aggcgagaga aaaatttgct ttcctcagtc ggaagagcat ttaattcgtc cgaaagaatt
     4081 ggattgcgat gtggtcagat ttcagaatgc caaagataaa tggattgctt ttgtagggct
     4141 gaaagatggg cgtccttatg aaatttttac cggattggct gatgacgaag aaggaattgc
     4201 attgcctaaa accgttactc agggaaaaat aattaaaacg gtggacgaaa acggcaacaa
     4261 aagatacgat tttcagttta ccaacaaaag agggtataaa acaaccattg agggcttgtc
     4321 ccataagttt gacaaagaat tttggaatta tgccaaactg atttcaggcg tgttgcgtta
     4381 cggaatgccg attgaccagg taataaaatt ggtttcggga ctgcagttgg acagcgaatc
     4441 aatcaacact tggaaagttg gagtagaacg tgccctgaaa aaatatattc cggatggaac
     4501 agaagctaat ggtaaaaatt gtccttcctg tggacaaaaa acactgatct atcaagaagg
     4561 ttgtttaacc tgcaaaaact gtggatattc gcattgcgga taaatataat ttgagtccgc
     4621 tccgaaaagt tttatttttg aaaattcgct tcgcccaaac gataaagaaa actcattttt
     4681 cagcaaccta catttaaatt aagggcaaaa agttgctgaa tttagctttt ttgacttttc
     4741 ggagcggact ctaatttgat ttgtctttgg gaaaaaggaa catttaaaaa gattgtttaa
     4801 acttttgtct tgtttttaaa aaacttacaa aaagactttt aaatatagct tttaattgta
     4861 tgttgctgcg tgtaggggaa agttgatgtt ttatgatttc gggctatcgg ttaatcaaat
     4921 tcataaattc ctcacgtgtg cgcgcttcct gaaaaacgcc tgtaaaatcg gaagtggttg
     4981 taatagaatt ttgtttttgt attcctctca tctgcatgca cagatgctgc gcttcgataa
     5041 taaccattac ccccatagga tcgagcgtgt tttgaataca ttgttttatt tgtgtggtca
     5101 tacgttcctg tacttgcaat cgacgggcat aaatttctac tacgcgtgct attttgctca
     5161 aaccggtaat ttttttgttg gggatatagg caacatgcgc ttttccgaaa aaaggaagca
     5221 aatgatgttc gcaaagcgaa taaaaatcta tatccttaac aataaccatc tgacggtagt
     5281 tttctgtgaa agtggccgtg cgtataattt cttcagcgtc ctgttcgtaa ccttgtagga
     5341 aaaactgcat ggcgcgtgcc actctctgag gcgtttgcag caaaccctga cggtcggggt
     5401 tttcgcccag cagacggata atttcctgat aatgttgtgc aatttctcct gtcttttttt
     5461 cgtcaaaagg acgggaactg gtaatgtcga aatccatgaa actgattttt tttcaaaaag
     5521 ctattttaca atctgatctc tcaccacata agtctgactt cgcaggtcgg gaaagacttc
     5581 cagcagcttg ttgcgaaagg cttgcgcttc atctaccgaa ctgaagtcgc ctacgcgaac
     5641 tttccagaaa ggcgagttgt agttgacata aacgccggta ttggggaaag cctcacgtat
     5701 ttctttttct attttgaaag cttcaacttt tccggtgcgc tgttcgttgc tcgaaaaaac
     5761 ctgcacgcgg taaccattcc ccaacgtcgc ttcacgaccc gttttggcgg tttgcttttg
     5821 attgataaga ttttctatgc gcttgtcttg atgaatgata actttgcctg ctccatccga
     5881 tgcttgaaga acttcaaaaa tattctgtcg aacagatgtg ctttcatcct gagcctgaag
     5941 cccgccagac actgcgagaa aaattgtgaa aagtaaaaat ttaaaatttt tcatcatttc
     6001 attcatgatt ggagtgcaaa gttataaaat atcgggtaaa tccgatagct tttcgacgaa
     6061 attatgaaat aatggaaaga ttgaactaaa tttggtcatc attttttgaa gtttaatgtt
     6121 tatctttgtt cgtaactatt tttcccaatt tatggaactt acaaaaattg tgcttattgt
     6181 tgccggcgga aaaggtgaac gcatgcagtc cgacattcca aaacagttta ttgaaatagc
     6241 gggaaaaccc atattgatgc acaccattga agcttttgca cgttacgatt tcgatttacc
     6301 tgtatttgtg gttttgcctg taactcaaat tgaattttgg aaaaatcttt gttcgaaata
     6361 ttctttcaaa ttttctcacc aagtggttat tggtggcgaa acgcgttttc attccgtaaa
     6421 aaacgggttg gaagctgttg ctgctttttt agcgtcgctt tatccggata aatctgagga
     6481 agagaataat tcagcattgg ttgctgtgca cgatggtgtt cggcctttgg taagtgtgga
     6541 gaccattcgg cgttgttttg acgaagccga aaaatttggt tcggccatac cgagtatgga
     6601 tttggttgat agccttcgca caattcatac caagtacagc caatcgttgg acagaagtgc
     6661 ctacaaactg gtgcaaacgc cgcaggtttt tgattttaac attttgaaaa aggcttatga
     6721 gcaggagttt tctccacttt tcaccgacga tgcttctgtg gttgaagcta tcggtgtaaa
     6781 tgttcatctc accgaaggaa atcgggaaaa tattaaactg actacagctt ttgatttgga
     6841 attggcatct tttctgttgg cgaaaagaga aaacagcagt tattgatttg gaaaattttt
     6901 tgaagatttt ttacataagt ggattttttt acccaagaca tacaatttat tccgggcgtt
     6961 ggccccaagc gggccgagct tttcaaaaag gaattgaaca tccgaacggc tgaggatttg
     7021 ctgagacatt atccttataa atacgttgac cgaagtcgtt tttacatgat cagcgaaatt
     7081 tccgaaaata tgcctttcat ccaactgaaa gggaaaattc tgaagtttga gaaggttggt
     7141 gaaggatgca gtcagcgttt gattggggta tttaccgatg gaaggcaata tatcgagctg
     7201 gtgtggttta aaggtttgaa atttgtagca gaacgttata aaaccaatat cgaatatgtt
     7261 gttttcggaa aacctacgct tttcaacgga aagtacaata tagttcatcc cgacatcgaa
     7321 attctttcac aacttccccc ggttgagcaa atgggcttac agcctttcta caatacttcc
     7381 gaaaaaatga agactcattt tctgaattcc aaagccattc agaaaattat ttattcactg
     7441 ttgccgactt tgcaaaatgg aattcctgaa accttgccgg catacattct gaatcgttat
     7501 ggattgctta acctgaaaga tgccttagtc aacatacatt ttccaaaaaa cagtcatttg
     7561 ttgaatcgcg cacgacagcg actgaaattt gaagaacttt tttacattca gttggatatt
     7621 gtgaaacaaa ctgcatggcg aaatcagcgg tttaaagggt ttgttttcaa acatgttggc
     7681 tattatttca atactttcta tcgcgattat ttgccttttg agttgaccaa tgcccaaaaa
     7741 aaagtcattc gagaaattcg ctccgatgtt gcttccggca aacaaatgaa cagattgctt
     7801 caaggagatg tgggaagcgg gaaaacgctg gtagcacttt ttgccatgct gatggcggtt
     7861 gataatggtt ttcaggcttg tatgatggct cccaccgaaa ttcttgccca tcaacatttt
     7921 gaaagtttga agcagttttt gggaaatatt ggtatccaaa ttgctttgct cacgggttcg
     7981 acgcgaccaa aggaacgagc ggcgattcac gaaaagctgc tgagcggtga attgaatatt
     8041 ctgctgggta cacatgcctt gatagaagaa acggttcagt tcaaaaatct ggggttggtt
     8101 gtgattgacg aacagcatcg ttttggcgtg gctcagcgtg caagactttg gagtaaaaat
     8161 gttcaacctc cgcatattct ggtaatgaca gccacaccaa ttccccgtac cctggccatg
     8221 accatttacg gcgaccttac tgtttccgtt atcgatgaat tgccgcccgg cagaaaacct
     8281 gttcagactt ttcatttcta cgacaacaaa aggcaggcgc tttataaatt tatggtgaaa
     8341 caaattgaag ccggaagaca gatttatgtt gtttatccgt tgattcagga atctgaaact
     8401 atggatttaa aaaatctgga ggaaggttac caacatttga gcgaaatttt tccgcaatac
     8461 caaatcagta tggtacacgg taaaatgaaa cctgccgaaa aggaagccga aatgcagaaa
     8521 tttgttgccg gaaaaaccca aataatggtt gctactacag tgattgaagt tggggtgaat
     8581 gttcccaatg cttcggtaat gattattgaa aatgccgagc gctttggttt ggctcagttg
     8641 catcaactca gaggcagagt gggaagagga gctgaccagt cgtactgcat tttgctgacg
     8701 ggatacaaat tgagcaattt gacccgcaaa agaattgaaa ctatggtgaa caccaatgat
     8761 ggttttcaga tagcagaaac cgatttgcaa ttacggggac cgggcgattt ggacgggaca
     8821 cagcaaagcg gattaccttt cgagttgaaa attgccaatc ttgcacagga tggaaaaatg
     8881 ctcgaaatgg cacgaaaagt agctatggat attatagaag aagacccgca gttgcagaaa
     8941 aaagaaaatt taatactctc tgaacaatta aaaaaaatga aaacctcatc cttaaattgg
     9001 agcgttatta gttaaaaaaa actaataagg ttttgttttt gattcatttt tttttatttt
     9061 tgggaaacca aaaatctcct gtatataaaa cttcctttca ttacaattta aattaatgga
     9121 aaaaacgctt gttattatta agcccggtgc cattcagcgc gaactgatcg gtgaaatcat
     9181 tacccgattt gaaaggaaag gattgcattt gtgcggaata aaaatgtatc agtttaccga
     9241 caaagtggtt tctgaacatt atgctcattt ggccgatcgg ccttttttta agcgtatcaa
     9301 ggattcaatg atggttacgc ccgtgattgt ttgttgttgg gaaggactgg acgccgttag
     9361 aattgtgcgt tccatgtcag gtgtaaccaa tggacgtgaa gcagctcccg gaacaataag
     9421 gggcgatttc agcatgagcg tgcaggagaa tattgttcat gcttccgata cggtagaaaa
     9481 tgccaaggta gaaattgacc gttttttcaa acctgaagaa atttttgagt ttaagcccaa
     9541 taattttcct tttgtttatg ctaatgatga agtttaattt ttttattaaa agaaaagttt
     9601 tgatgatgga gtatatttcg aagtgtttaa aattcttttt tttattgctt ggttttttgt
     9661 tgttttgcaa tgcaataaac gcagaaaaag ttaatattcc caactcgaca acttcaaatt
     9721 taaaagatac tcttaatatt taccaccaaa tgttgaatga cacatctgat gatttgatgg
     9781 aaaatcaccc ggcagacgat atttacaaca acatttggac ttccgaaagg ttaaatccct
     9841 atcattttcc ggtagatagt ctgcctgatt ccgtaagaat tgactgttcc aatttcattc
     9901 tacctattta cggaaaagta acgtctaaat ttggccgccg tcgatatcgt tatcattacg
     9961 gaatcgactt aaaactaaat gtaggcgatt cagttcgttg tgcctttgaa ggaaaggtac
    10021 gcattattga ttatgaacat ggtggttatg gtcattatgt agtagttaga cacaataacg
    10081 gactggaaac ggtttatgct catctttcaa aagttctcgt agaactgaat caaataattg
    10141 gagctggaga ggtaatagct ttaggaggaa ataccggtcg ttccacagga ccacatttac
    10201 attttgaaat gcgttatttg gggaacgcca ttgatcctga taatttggtt gactttgaaa
    10261 aaggttccat aagacaattt tcttacctga ttaccaagaa aaatactttc aaatatcaaa
    10321 aagatttgaa aagtcttcaa atggcacgct accatgttgt tcataagggt gatacactta
    10381 gtcttatagc ttcacgctat ggcacaacgg taaaacaact ttgcaattta aatcagatat
    10441 catctaaaaa gttattaaaa cccggtcaaa aaatcagagt tcgataggag aggggaacta
    10501 tgaaatgctc tcctttttag gtaatgaaaa attttttgct tatttcacag gttagaataa
    10561 tagttttagg ctcttcatgt aaaagagagg cataaagtag attaaatcct cgcaacaatt
    10621 atttttcctt ttttataatt cgcaattact tcctttacat cattttgcat gcaaaccata
    10681 tctatgcctg ttagccattg tttagttttg gtttgacctg caagcatcat tttcgattcg
    10741 attgatgaaa aggataatca aaacctcaaa aagtataact tacttttcct aaatcaaacc
    10801 aaaaccacat gaaaattctt tttgctgtgc tttaaagtat ttctgtatta tatatgttca
    10861 aaaaaaaaat ccctgtttct aattttaaga aacaagggat gttgtaagaa taaaatttag
    10921 gaaaaattac ttttcgatcg caagtaattc aacttcaaaa atcagcatgg aaaaaggttt
    10981 tatggcacct tgattggcac ttccgtaagc caattggtaa ggaacataaa ttttaaattt
    11041 ggaacctacc ggcatcaatt tcaatgcttc agtccaacct ttaatcacct gattgacata
    11101 aaattcggca ggttgatttc tttcaacaga gctgtcgaaa acagttccat caatcaacgt
    11161 cccatgataa tgaactttga ctttgctgct gtcggttggg ataggacctt tgcctttttt
    11221 gatgatttcg tattgtaaac cactttctgt agttattact cccgattttt ttgcattttc
    11281 aagcaaaaac ttttcaccgg cttccttgtt ggcactatat tgtttttcta ttttcttttc
    11341 ctgaagactt tgcatggtag attgcaaata ttcgctggct tcctgaggag tcatttggat
    11401 gttcgagcct ttcatgccat tgattaaacc ttgtttaacc aaattgatat tgacagataa
    11461 agaagagtcg cccattaaac cacttttgct ctgttcttta agagagtatc caatttgaga
    11521 accaatactg gcaagttcca tgttttcttt gtccactttg gattttaaac catcgttgat
    11581 tccttttaac aaagaattta aagctttgcc ggtagaatcg ccctgtaaat aataattttt
    11641 gattccatca ccattggcaa cgccaaaagc ataattcaaa ctgtcgattt gcgatttaag
    11701 agtcggcttg ccgggtttat cgctgttaca tgaagaaaaa gtgaaagtag ctgaaatggc
    11761 taccagaaga aataaattta attttttcat cttacaaaaa ttatatgaga ttttattgta
    11821 atgtcttaat tcatttgaat tactgcacac tgtcagtttt ttgactttct ggagccggtt
    11881 gaggctgatt agcaggtgct tgttgagccg atttttttgc ttgtaactca gtcattgttt
    11941 tttgtaaata ctcctgagct tgttgaggtg tcattccacc atcgaaaccg tgaagtccgt
    12001 tgacaagacc gagtttaaac aacttggtat tgaaaaccaa cgtggaatca cccatcaacc
    12061 cattttcttt ctgatacttc agagaagttc caatctgata acccaactga tacatctgat
    12121 catctacttc tttactggaa taagctttgt ccaaagcatt tatcaaagct ttaatgggag
    12181 catccgatga gtcgttttgt aaatagtaat tcttaattcc ttctccattg gcaagaccca
    12241 acgtgtaatt gagactgtca ttttgagttt ttaattggac tttttttgct tcgtattttt
    12301 tactacaaga agccaacaaa aaaacggcaa gaactgttgc caacataaaa ccgatttgtt
    12361 ttttcatttt gactgactta tttttttatg atacaaaaat ttttagttta ttttttagct
    12421 ggatttattt gcaataattc cacttcgaaa atcagtggag aataaggagg aataacgcct
    12481 ccggcaccct gctcgccgta agcaagctca tatggaatat aaaatttata tttggaaccg
    12541 acggtcatca gttgcaggcc ttcactccat cccggaatta cctgattgag agcaaaaact
    12601 gcaggttctc ctctgtcaaa agtactgtcg aacttggtac tatcgggaag gaaaccttga
    12661 taattaacct caaccgtatc attgatagat ggttttggac cattggcaca tgcaaggatt
    12721 tcgtactgca aaccactttc agttgttatt acactttctc tggttttgtt tttttccata
    12781 aaagcatcag caacttcttt tttctttgcc gactctaagg attgagcttt agtaatgtat
    12841 tcacgaaaat aattgttggc aaaagcagtg gtcattacag tagaatcttt tttcataccg
    12901 ccggtaaatc cctgtatcaa ccaatcaata ttaaattgac cacccggaat ggttttcaaa
    12961 tttttaataa aatcagatcc aatgctcaaa cccaaagcat aactcatgga atcgatagtg
    13021 ttggtgagtt tctgaaccgg aggttcttct tgaatttttt tgggcttttt ggcctctgcc
    13081 gaagttatca agaaaattgc caataaagat aaaacaaata tttttctcat ttttatagtg
    13141 aataattgat ttttagaata ttaaaacttt aaaaatttca gcaaagataa ctgatttatt
    13201 gcattccaac atgttaattt ttcaaaatta tcgtgcagag accacaattg gaattttcat
    13261 ctgctttttt tggccggttt ttggttgaat catctcaaaa tagagtacat aaattcctgc
    13321 attagcaatt ttcccgctat cggtgcggcc atcccaagaa aagaaacctt ccgaggatag
    13381 caacgcattg ctaaccaact gaaaaacttt tgtcccggtc gcattaataa tttgagcgtt
    13441 agccacaaaa ccttctgtcg gagtacgata ttttatgaaa cagacatcat ccgtaccatc
    13501 gttatcggga gaaaaagctt cgggttcaac ccaaacgaat tgtttgtttg aaaaagtgtt
    13561 ttcgatttca cgatattgtg aatttttata gccgggtgtt ccgtatttaa cttcagaggc
    13621 tgccgagtgc caactttccg ccgattgtgt gggcagagcc ggattaattc gctccaatgc
    13681 cacaccctgc cggtttttta tcattaggtg atgccatttt tcattgtacg tcaattcatc
    13741 atatacagta tcggctttgg aattggcaag cacaatggta ctttgctgat tgttgagtgc
    13801 cgtccacgaa atggaaacga tactgccatc gtttggcagg ccaaaataat tcattaccga
    13861 atcggcatgc tgacatacag ccaaatagcc tccggctgcc aacaatttac cgtcaggaat
    13921 atggcgcaaa gtattcaatg aaccatccga tttgcgtgta gttatgacta ttcccgaaac
    13981 atccagcgtt ttggctgaag cattgtagat ttcgatgtat tcagtagcag taggaggtgc
    14041 attaaacatt acttcgttca gcaataaatc tccctgcgct aaaggttcaa taataccggt
    14101 ttttttgata ttgtcaagta acgtatttcc tgccaaatcg gttacttgca aaagttcaag
    14161 cgtataaatt tttcccttat caaaattttc gttcagcgca acattcatca aaaaaggttt
    14221 ggttgataaa atatcgattt ttgtaggagt aatttcattt tcgttcaaaa tgaaaattga
    14281 attggacaag tcaaccggtt cagaaaattg caactgcaag gtatcaggtt caattatggc
    14341 aagtttttcc caaacaggtg gttttttatc cacagctttt ttcccgttag cagcaatatc
    14401 atcgaaaaaa taagctgagg ttgtagtcgt tgtattggaa taaagcaaac caatatatga
    14461 gctgctttcg atagttgtat cgcgaaccgt tccttccagc acaaaatcac tttcggaagg
    14521 gagtttactg taaagagaaa agtaacccag tgaatcgcgg gtaactttta cccgaatttc
    14581 aacctgtgta ccatctgtgc gcttatctgt gccatcaatg attttgattt ttgacgttcc
    14641 ttgctgtctc cacaacgaaa catcgtcctg cgtatcacca atttttacaa aataagcatt
    14701 tcctaaatta tcgggagaac tcgaaataat gtaaaaagcg gaataattgg aagaagaagt
    14761 ggaataatta attttaaacc atgcttgcca ttcggcatcg taaaaaactt ccgatggcgt
    14821 aaacaaatag gaaacggaag ttgttggagc attggattgc aactgaaaat attcgtttat
    14881 cttaaaattt cctgtcgttc cgctccatga tggattttca gtaaaattcc catcagaaaa
    14941 gtcgtcaagt acttgtccga aagaaaagac aggcagtaaa tacataaaaa taagacaggt
    15001 tttcttcatt ttttatgaaa attctatgat ttagattaat tttgtaccga aaaaagaaga
    15061 agaatcaatt ttttaagaca atatactgca aaaataaaaa tttcttaaca aagaaaatta
    15121 tttgcgtgca tatttctttt taccaaacat acaaataacc aaaattttac aacaatgaga
    15181 gtagcaattg taggcgccag cggagctgtt ggccaggaat ttttgcgcgt tttagccgaa
    15241 cgcaatttcc cattgacttc cttgagcctg tttggctctt cccgtagtgc gggcagcgta
    15301 tatgatttta aaggagaaaa acttaccgta aaggaattaa aacataacga cgattttaaa
    15361 gacatagact tggcatttac ctctgcagga gccggaatat ccaaagaatt tgccgaagat
    15421 atcaccaaat acggggcatt gatgatcgac aattcatctg cttttcggat ggaaaatgac
    15481 gtgccgttgg tagttcctga agtaaatgct tccgatgcaa aaaatcgtcc ccgcggcatc
    15541 attgccaatc ccaactgcac taccattcaa atggttgtag ctttaaaagc cattgaaaac
    15601 gtttcacaca tcaaacgcgt tcatgttgcc acgtatcagg ctgcaagtgg tgcaggtgcc
    15661 gctgcaatgg ccgaattgga actgcagtac cgtcaggtat tggctggtga aacaccgaca
    15721 gtgaataaat ttccttatca gctggctttc aatgtaattc ctcaaattga tgtgtttact
    15781 gaaaacggct atacaaaaga agaaatgaaa atgttcaacg aaaccagaaa aatcatgcat
    15841 tccgacgtag tggtgagtgc tacctgtgtg cgcgtgcctt cattgcgtgc gcattccgaa
    15901 agcatctggg ccgaaactga gcgtcctctg agcttggaag aagtgaaagc tgctttcgaa
    15961 aatgccgaag gtgtggtggt gatggataat ccggctgaaa aattatatcc catgccactt
    16021 ttcctgtcgg gtaaagatcc tgtatatgta ggacgtatcc gcaaggattt ggcaaatccc
    16081 aacggaataa cattctggtg cgtttcggat cagataaaaa aaggagcagc actgaatgca
    16141 gttcaaatag ctgaatatct gatcaaggaa aaagagtttt gatgaaggag aacttttttc
    16201 ttctacaatt ttaacaaaaa ccgtcataac atgttggtat ggcggttttt gttttcaact
    16261 tattttgttt ttgagattaa aaatcaaagt ttttcatgaa gttgcgataa aagggaggtc
    16321 aatttattct acaaaaaaga aatttaccaa aacaaaatct gagttttgaa tgttatcaaa
    16381 aatgcaaatg aaattttaga atttctgata caatttctaa aatttacaag caactttttt
    16441 gacaagtaaa aaatttttca atttatgtca gaagatataa taaatcaagt tacacaggga
    16501 gaatacaagt acggtttcac taccgacatc gatacagaag tttttccgat gggacttaac
    16561 gaagaaatta ttcgacttat ttcggcaaag aaaaacgaac cggaatggtt gctcgatttt
    16621 cgtatcaaat cctatcgcca ttggctgaca atgaaaatgc cgacttgggc tcatttggaa
    16681 attccgccca ttgattttca gaaaatatcc tattttgcgg cgccacgaaa aaaatcgcca
    16741 aaaagtatgg acgaagttga tcctgagatt ttgaaaacct tcgacaagtt gggaattccg
    16801 ctcgaagaac agaaagcgct ttcaggtatg gctgttgatg ccgtgatgga cagtatttcg
    16861 gtaaaaacaa cgttcaagga aaatctggca gaattgggta ttattttctg ttcattcagc
    16921 gaagccgtgc aacatcatcc cgatttggtt caaaaatatc tgggttcagt tgttccttat
    16981 tccgacaatt tttttgcaac gctcaatagt gccgttttca gcgatggttc ttttgtgtac
    17041 attcccaaag gtgttcgctg tccaatggaa ctttccacct actttcgcat caacgcttcc
    17101 aataccggtc agtttgaaag aacactgatc gttgccgacg aggacagtta tgtttcatat
    17161 cttgaaggtt gtacggctcc aatgcgcgac gaaaatcagc ttcatgctgc cattgtggaa
    17221 atcgtagcac tcaaaaatgc cgaagtaaaa tattctaccg tacaaaactg gtatccggga
    17281 gacaaagaag ggaaaggcgg aatttacaat tttgtaacca aacgcggcat ttgcaaggga
    17341 gaaaattcca aaatttcgtg gacacaagtt gaaaccggct cggccattac ttggaaatat
    17401 cccagttgca ttttacaggg cgacaattcg tcggccgaat tttattcggt agcggttacc
    17461 aatcattttc agcaagccga taccggcacc aaaatgattc atttgggaaa aaacacccgc
    17521 agccacattc tctcaaaagg aatatctgca ggtttcggcc agaacagcta tcgcgggttg
    17581 gtgcgagttt caaaaaacgc cgacaatgct cgaaattttt cgcaatgcga cagcctcctg
    17641 ctgggcgata aatgtggtgc tcatactttt ccatacatgg acgtcaacaa cgatacagcc
    17701 atagtggaac acgaagccac cacttccaaa atcagtgaag accagatttt ttactgcaac
    17761 caaagaggaa tttccactga agatgctgtt ggcttgattg taaatggtta cgcaagggag
    17821 gttatcaata aattgcctat ggaatttgcc gtagaagcac aaaaattatt acagataacg
    17881 ttggaaggtt ctgtgggata aaacctgtta accttttgaa agaaattttt attacagatt
    17941 attagaaaaa tacattatgt tggaaataaa agatttacat gcatccatca acggaaaaga
    18001 aattttaaag ggagtaaact taacaattcg tgccggtgaa atacatgcca ttatgggccc
    18061 gaatggggct ggaaaatcca ctttggcgtg ggttttgtcc ggcaatccgg ctgtggaggt
    18121 aacacacggc gaaattattt ttaacggcaa aaatttgctc gaaatgtcgc cggaagatcg
    18181 ttcgcgcgaa ggaattttcc tgagttttca atatccggtc gaaattccag gtgtcagcat
    18241 ggtcaacttc atgcgtaccg ccgtcaatga acaacgtaaa tacaaaaatc tcgaacctct
    18301 ttcggctgcc gattttctgc gcttgatgcg cgaaaagaaa gaattggtcg aaatggacaa
    18361 taaactgacc agccgttccg tgaatgaagg tttttccggc ggagaaaaga aacgaaacga
    18421 aatatttcaa atggcaatgc tggaacctaa attggcaatt cttgacgaaa cagattccgg
    18481 actcgatatt gatgcgctgc ggattgttgc caacggagta aacaaactca agcggcctga
    18541 caatgccaca cttgtgataa cccattacca acgcttgctc gactatatta ttcccgattt
    18601 tgtacatgtg ttttacgacg gacgaatcgt aaaaacaggc gataaaaact tggcacttga
    18661 gctggaagaa aaaggttacg attggataaa aaaggaagtt gaaactcctg tagagcagta
    18721 aaaattcttc ttaactatcc gaaatttgaa aaatcatgga acaacaatat atcgattttt
    18781 ataaagaatc tcataccctg ataaagcaac attcggctgc tttgatgaat aattttcgcg
    18841 atgccgcctt tgagaaattt gaacggctcg gttttcccac ttcaaagctc gaaaactata
    18901 aatataccga catgtcggca cctttgtcga tagaatatgg gctgaacatc aaccgattgc
    18961 agtttcaaat caatccttac gatgttttta agtgtgatgt gccggctatt cattcctact
    19021 tgttttttgt gttaaatgat actttttacc ccgtcaacag cactgccaaa cagcatttgc
    19081 cagaaggcgt aattattgaa agtctgcgaa cagctgcaga aaattatcca caactggtag
    19141 aaaaatatct tggaaaatta agcaatgaaa aaaatgacgg ttggattgct ttcaatgaaa
    19201 ctttcgctca agatggattt tttatgtacg ttcccaaaaa cgtgaaactt tccaagcctg
    19261 tacaattgat aaacataatg cgctccgacg tggatttcat ggccaacagc cacaatttaa
    19321 tcattctgga agaaggagcc aaagctcagt tgctggtttg cgaccacact atggacaatg
    19381 tgcacttctt ttccaatcgt ctcaccgaag tttttgcagg aaaaaatgct gtttacgaac
    19441 attataaact cgaaaacacc aatatcctca acaccaatgt ttcaaactta ctgattcatc
    19501 aggaagattc gtcgaatgta ctgacaaacg tcattactct tcataatggc atgacacgca
    19561 acggcatcga aatgataatt gatggagaaa attgtgaaag tttgctttgt ggcatggtga
    19621 ttggcgataa aaatcaggat gtggataatt tcactgaaat tgttcacaaa aaaccaaatt
    19681 ctcaaagtcg cgaattgttt aaatatgttt tggatgattc ttccaaagga gcatttgcag
    19741 gaaaaataca ggttgcaaaa aacgcccaaa aaacttcatc ctttcaaacc aacaaaaata
    19801 ttctcctcag caaaactgcc aaaatgcgaa ccaaaccgca actcgaaatc tatgccgacg
    19861 atgtgaaatg ttcgcatggc gctaccatcg gacaattgga cgaagcagcc aagttttaca
    19921 tgcaatcgcg tggtatttcg gagagcgaag cacgtttgct gctaatgttt gcctttactg
    19981 cggatgtgat ggacaatatc cgtatcgatg ctttgagaga cagaatgaaa ttattggtag
    20041 aaaaacgtct tcgcggtgaa ctttcccgat gtgaaggctg catgatttgt tgactttttc
    20101 ggtgaaatta ttgtaattct ctgttttttg taaaatattt ttgtcgattt tctccggtat
    20161 ttttttgtgt tgattaataa atttgggtca atttttttat ggggaacaat ttatgttttc
    20221 tgataacaca tcgatttaca acttttcatt tattaaaaaa ggctgccggt tttgttccaa
    20281 cagccataag gaattaagaa ataaaaatca aatgttggaa agggcttgct caatatcggc
    20341 tttgatatcg tcaatatttt cgattcccac tgaaaaacgc agtaatcccg gaaaaactcc
    20401 cgctgcaatc ttgtcttcgg gcgaaagttg ctcgtgagtt gtggtagccg gatgaataat
    20461 aagagatttt gcatcgccca cattggccaa atggcttatc agctttaagt tgttgatgaa
    20521 tttgtctgcc attgaagcat cgcctttcaa tttgaaagcc aatacgccgc caaaaccttt
    20581 tttcaggtat ttttttgcca tttcgtgata aggattattt tccagaccgg gataattaac
    20641 atattccaca gccgacaatg tttgtaacca gcgagcaagt tccaacgcat tttggacatg
    20701 tcgttccatt ctcaaagaca aagtctccaa accctgcagc aacaaaaacg aattaaaagg
    20761 actgataacg catccccaat cgcgaagacc ttctacacgt gctcttatat tgaaagcaat
    20821 attcccaaat gggctatctg ctccgaaagc atcccaaaaa accaatccat gataactttc
    20881 agaaggttcg gtaaaagccg ggaatttgcc atttccccaa ttaaacttac cactgtccac
    20941 aattacacct cccagcgaag taccatgtcc tccaatccat ttggtagcgc tttctaccac
    21001 aatggaagct ccatgttcta tgggacggaa aagatagccg cctgcgccaa aagtattgtc
    21061 cactatcagc ggaatatcat atttatcggc tacagcagca attgcttcaa aatcggggat
    21121 attcaaatcg ggattgccaa tggtttcgat ataaatggct tttgttttgt cgtcaatcaa
    21181 tttttcaaaa gtttccggtt cgtcatttgc cgtaaactta acgtccacac ccagacgttt
    21241 aaattgattt ttaaactgat tgtaagttcc gccgtaaaga tgagaagttg ctacaaaatt
    21301 atctccagcc tgtacaatat tgtttagagc aagaaattct gcagcctgtc ccgatgaagt
    21361 tgccaatgcg ctaatgcctc cctcgagtgc agccatacgt ttttcaaaca cgtcggtagt
    21421 aggattttgc agacgggtat agatatttcc aaattttctc aaagcaaaaa ggtcggctcc
    21481 atcggctgcg tcgtcgaaaa cgtaggaaga agtctgataa atgggtactg ctcgcgattt
    21541 ggtagttttg tccacttcct gaccggcatg aacctgcaag gtttcaaatt ttaaattgtt
    21601 gatattcata ttgctaatta tttagatttc gattcttgaa atttataaag gatattcgtc
    21661 aaaagcataa agtgcagtgg aaaggtatct ttcgcccgta tcgggcaaca gaacgacaat
    21721 gttttttcct ttgttttcgg gtcgttgaga caacaaagaa gcggcaaaag ctgccgcacc
    21781 cgacgaaatt cccaccagca agccttcctg ttgagccaat tggcgtgaag ttcgaatggc
    21841 gtcatcattg ctaacctgaa taatttcatc tacaaccgtc gggtcaaaat ttttgggtac
    21901 gaaacctgct cctattccct gaatttgatg ggggccgggt ttccctcccg aaagtacggg
    21961 agaacttgca ggttcgaccg caacaatttt tattgcagga ttatgttttt tcaacgcagc
    22021 tcctactcca cttatcgttc ctccggttcc cacaccggca acaaaaatat cgactttccc
    22081 ttccgtatca cgccaaattt cctcgcctgt ggtatgaaaa tgtttcaatg gattggcagg
    22141 attttcaaac tgctgcggca taaacgaaga tggattctga tcatgtaatt catgcgcttt
    22201 ggcaattgca cctttcattc cttcggtccc gggcgttaaa acaatattgg cacccaaagc
    22261 cttcagtaaa ttacgccgtt caacactcat ggtttcgggc atggtaaggg taagtttgta
    22321 acctttcacc gtagcaatca tagccaaacc aatacctgta tttccgctgg taggttcgat
    22381 aatttcagaa ccttgtttta aaagtccttt ttcctctgcg tcttcaatca tggctaaggc
    22441 aattctgtcc tttacgcttc ctgccggatt aaatgactcc agcttggcca taatattggc
    22501 ttgcaatcct ttatttgaaa caaaatgtgt taattgcaac aaaggtgtat tgccaataag
    22561 ttcagtaaga tttttagcaa ttttcgacat aaacttttga tttttaatga atttacaaaa
    22621 tcttttttac cgaaaaaaat atctgactaa agattttgtt tttcttttgg caaaggtaca
    22681 aaacaactaa aaacaagtca atcacaagaa tgggttaatt taatctacca cgatgatggt
    22741 atagtcaaaa tttcaaataa caataatgga gaaaattagg attcaaattt atataataat
    22801 ttgtaaatca agaatataat taattagaga aacaaacgaa aagaatttcc actttgctta
    22861 ttatgaaatt cacatgtatt acatacacaa acaagaatga atataatatt cagacatgtg
    22921 ggttccatca gacctttaaa aaataaatt
//
LOCUS       DAOTAX010000029        22373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1904_length_22373_cov_27.849583, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000029 DAOTAX010000000
VERSION     DAOTAX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_22373_cov_27.849583"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1428)
                     /locus_tag="PLM04_04675"
     CDS             complement(253..1428)
                     /locus_tag="PLM04_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HON02403.1"
                     /translation="MKKLFLLIFTTFICLNGIAQKFDGLANTPPMGWNSWNKFNCDVN
                     EELIMKMADAMISSGMKDAGYEYIVVDDCWQVSRDENGNIQHDTQRFPHGIKYLADYI
                     HSKGLKFGIYSDAGIKTCQGRPGSRGYEFQDAKTYASWGVDYLKYDWCNTGTQDAKAS
                     YSIMRDALYAAGRPIVFSICEWGKSRPWEWAQEVGHLWRTTGDIVDRWDAMIDIVDKQ
                     RDLGPYSGPNHWNDPDMLEVGNGGMTIEEYKTHFSLWCMMAAPLMAGNDLSDMSKETL
                     EILTNKELIAIDQDKLGKQAFCFRDNGDYEIWIKKLSNDEKAVCLLNRSDEIKEVMVD
                     FAFLLKSNDDYWTQDPYQLQNYIVRDLWEHKEIKTDKYPTFVKIPPHGVKVYKYIKK"
     gene            complement(1613..3064)
                     /locus_tag="PLM04_04680"
     CDS             complement(1613..3064)
                     /locus_tag="PLM04_04680"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HON02404.1"
                     /translation="MNNRSSALDALRGLAIIGMVLSGTVAPTLPAWMYHAQVGPRSNF
                     NFDPTIFGITWVDLVFPFFLFAMGAAFPLALGRKLDKGATVTSLFPAILKRVFLLVLF
                     AIAIYHCSPYRLGGGWNFALAILAFGLFFISFVRFPNVSEKKNTWLNWLGIAFLMGLI
                     AFNVSSQPEVFSKGFKLSHNDIIILVLANMALFGSLAWILTRTNLLLRLGILAFLFAL
                     RLTSGVDGSWNQVLWNFNPMQLLPDSITTIVYNPGGWLYRMDFLKYLFIVIPGTIAGD
                     LLVRRFTDKDNEKSILPSKQKMGASLLLMVAFVLSNLICLFGRFLLLNLILNIAFSIA
                     GYILMRKPTHSLSRLYFSLYKWGIFWLMLGTAFEAFEGGIRKDSATMSYFFVTSGLAI
                     FTLLFFSFVIDYFENKKLFRYISECGQNPMVAYVAGTFFVVPILVFTGLMPHINTLHE
                     ITPWFGILKGVIITSGMMAVTVFTVRRKWFWKT"
     gene            complement(3087..5102)
                     /locus_tag="PLM04_04685"
     CDS             complement(3087..5102)
                     /locus_tag="PLM04_04685"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HON02405.1"
                     /translation="MKKITLLLISFYIAFNADAINAVVYDGSGQLSEDKLIALTLAGI
                     VNRDAPRLYLRNVYETWSFNQTDELWENEYKTVGGVQFTVITNINALVQYFKSYIKGA
                     ITYDATLTYSNFSGQSFRWQAEAAAMLGGLTDCIPVPSTNTIIDINKPTSVDLPDYFN
                     GQQTVPVSAKLELTSHSWNNTALTQEQRYFAWLDWALDNLLPRCNTSKFYLREITDWA
                     VNQRMFQLNLAGTEDLRFTSLSDEKAAKIERVMNYLKTKRPNEIFHVYGWMRPEPLVQ
                     WISAWGGSFHETLLSNLSWHHVFPVDANFSYQRPADINPELVLLEDKYYVLFISSEGD
                     AGNWNIGFQGGAWASTTRGQVPVAWGFNLQMFEEFPFVAQYYYRTATANDGFMAVSTP
                     LGYAYPDVFPTSYLPDAKAKTIALMDKFNVNSVYAYKHYNGAGSSQYRGITISNNFNF
                     SNLGLFAEQTNAQMTMLFDPALMTQKAYTNYGGLLYNHVNDDTFYADFSNLTTVRDRI
                     VSKLSGKTKPRFLMAGYQRFRFDNATLATNDVTLPRLKTLMDDIKNNTSIGADVEFVT
                     PEKFTYLLRKSLGLSTTVPAVENNGQKLLVFSDQNNRLQLNLQLESAQLVQIRVFDLA
                     GKLILSENWNMTMSNDFKTISVAQKGIFILNIKGQNVNLTSKIVNGK"
     gene            complement(5175..6971)
                     /locus_tag="PLM04_04690"
     CDS             complement(5175..6971)
                     /locus_tag="PLM04_04690"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HON02406.1"
                     /translation="MFSTFYSCNEPDLSNPKAPFPAPTELVVEKITTTSVKISWSDNS
                     NGEDGFTIERWVTGTSVPVKATVGANTTEWIDEGLQEATYLYKVYGFCKQRVSDTVSV
                     YYQHVPVSMPTNFTVVAAHNTVNLTWDALTENIDGYKIERKIGTEIYQAWKTLDANTT
                     SVTDSDPAVGLNTYKLYAYAGSATSVGVERSLMILKTPIITIASPIVSYLKISAYFTL
                     TSDGGEPCNAGLCWSKSPNPTITDSKSVWHVKVKSGETFFGNAVNLDKNTNYYIRAYA
                     TNSKGTTYSPEIQATLSPELPAIALSWTPITSINAELPTEINVYSTTSSLNGRPFSAW
                     YAIADMSTGIIELKTTLSTSPQKPSQFIAAATNETTYVMTNAGYFGYSGSTVNSYSLA
                     VDRTSKKSDNISTLTRGSHSYAVTRGAFGVSQNQTPSIKWAVGNFAYDIPSPNVEGET
                     PQPSLSASFPAQSQNWNPYSAIGGAPVLLKDGKIVFDFTTTTSGKYMTNYELLQTDIF
                     STTARPPRTVIGSTADNKIVLFVCDGRQTHSDGATLIELEQIMKGIGCVNALNLDGGG
                     SSAIIAGGKLLNKPSDGTERGVATVVSFVKKK"
     gene            complement(7041..8693)
                     /locus_tag="PLM04_04695"
     CDS             complement(7041..8693)
                     /locus_tag="PLM04_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HON02407.1"
                     /translation="MKKFFLFFTCILLLSCKQSLEGGNEIISFKFQEISQANEAYISN
                     DTVLIMVPTNTDLTSLTPVIEVSVKATINPASGVKQDFSKPVFYTVTSELGTKKSYIV
                     LVYSQPTFRVNNVKLPDNFVTGTINGDNITFTVPFGTNVSAMKLTYLLADNGLTSNIA
                     SGSTVDLSQPFLWKLSIGNLSKTYTITANIQPQETGVRAVWVTNVASSVLFSESALTD
                     MVDKLAELNFNTICVCTYNKSQTLFPSQTLATVLGTTPAQTVFSGCSWGDVLEKLIEK
                     AHAKNIKVIAWFEYGFASHYSGQDSPISDKHPDWVSKQQDGTQTVKNSFYWLNGFHHE
                     VQQFMTDMIVECVTKYPELDGIQGDDRLPALPAHAGYDDYTKQLYKTETGRDVPSNPT
                     ETIFFNWKVNKMSDYAVSLYNAVKAARSTCLVTWAPSTRGWSRDNYLQDWPEWLRRGV
                     ADWISPQLYRNESQGVDSYTTLVYSDVQNVFNTADLRKKYVPGMLVRNGNTIVSDEFL
                     AQLIQYNRTKGVYGESTWFYEAIPSKQKVYKALYPGKAIFPF"
     gene            complement(8813..10243)
                     /locus_tag="PLM04_04700"
     CDS             complement(8813..10243)
                     /locus_tag="PLM04_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON02408.1"
                     /translation="MLPDKIKEKISEIGTDFNENKLESINILKGFKALKLTEGFSEFK
                     HLKRSGYSFQLVLSSMIYAVTPGRKTAVSSLPLLKEKGITMGKNILYRLKNNAGINWR
                     KLLWQTACKFIGITGQEKEVSCKVRCLIFDDTLLEKTGKKMEKIGKVYDHVTNTFKLG
                     YKLLLGLYWDGKSMIPLDFSLSREKGKRAEKPYGMSRKELRHQYSKKRLKELSGRERE
                     KELDKSKIEMMLEIFYRAVFYHIPLDYVLCDSWFTCQALITAVGSKHIHLIGMYRIVK
                     AKFLYQGKQMNYREIQSSIDKITRCRKMKLHYKRADVMLDGIAVTLFFTRQGKRGKWK
                     VFLTTDTKLSFVQLMEIYQIRWSIEVFFKETKQLLNLGGCQSNDFDAQIADTTISMIA
                     YILLSFRYRYEHYESMGELFRVMNAECLQQTLDERLWGLFLELLQEICEAFEKDIDEL
                     FELIMNCPKTAELVSKMLTPPLREAV"
     gene            complement(10551..12017)
                     /locus_tag="PLM04_04705"
     CDS             complement(10551..12017)
                     /locus_tag="PLM04_04705"
                     /inference="COORDINATES: protein motif:HMM:NF027725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HON02409.1"
                     /translation="MKTNRLFNKIAIVLFVALSLASCKNEPEVVLKTDSEIKSFALDL
                     SSLDALADQTIKASISVEIVGEEIQINLPFGLKPDTINRLLNSNAATLTVSEGATVSP
                     ASGTPVKFNSATPVYYTVTAEDGVSKTAYKVRFFETAQKGYGVISEYWRKNGTAFTDK
                     FTDNNEKTFALTPTHIVQQIGGLYGGTLKLVKVSLLDGSETGYAKLVVAPGDTIKQIR
                     AMAGDRKGSFIGCNLAAPGGDFKVWCWDNVDAAPRELIKWNVSDLTPVGTPPSWAGNH
                     VGRRISVIGDLKTQAYIYTYANYTLDILRWKVTDGVAASSPEFIHYTPGGSMSRFELW
                     ASIVPLGATATSDILVNAACAGSEVGVMNGITKQTYMFATAVATPKGIATYLEFNNVK
                     YYIPLNVEAYATPHSVNARLYDYTTTTNSVEMSNNWWLTPQPEKIEADGTLKNGDATG
                     CIDAILDADGLGFKVVALGTGCGIVVTKFSKKKELTFN"
     gene            complement(12078..13784)
                     /locus_tag="PLM04_04710"
     CDS             complement(12078..13784)
                     /locus_tag="PLM04_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HON02410.1"
                     /translation="MKNTIFILLVAVVFSACTDRFAEINTNPNASTTIDPAYLFTTSQ
                     RRGAMDDRVYYYTRGMACFHTQMYANTNYTKEHCTDRYQFNAAWNGTLWSAAYAGDAS
                     KTYLSFVAEAEKIAKQKGWENKEAICNIWKSYLFLQLTDLFGDIPYSEAFNDLIDYPK
                     YDKQEDIYIGIYNSLKTSVQKIDATKGNGMGSADLFYNGDYSSWKRFGNSVRLRMAIR
                     ISKVNPTLAKTWFDETLADAGGLMSNNLQSCRLKTDPAGPQGIHSDNPIKVMANAFDW
                     YRISEFMVKTLNELDDPRIFNFAEPTKNYLNALKQICKDIEQNSTIYNSLSYAEQAKV
                     SYAAAEGGSFINIFAQGRYFTDEIEAIVARVLPSRSSEISRSIKYRGLRNGQSIESLT
                     TLQSKLGEYSDLSQYLRQDNYPTNIFVYPEICFLLAEGNLNGWTTPKTAEEHYNDGVR
                     AAMQLYEVPDSEINKYLAGAAKFKSNGTKAEKLEQIILQKYLGNYTDGFEAYADLRRT
                     GYPKILPIEYTSSVPDEMELPRRWKYPSDEHLLNKANVTEAANRMGGDYQLSRMWWDV
                     NN"
     gene            complement(13812..17027)
                     /locus_tag="PLM04_04715"
     CDS             complement(13812..17027)
                     /locus_tag="PLM04_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HON02411.1"
                     /translation="MKKNVKLLIVLVFQLFAFGQINAQIVKGIVTDAKGEAIIGATVL
                     EKGTNNGTVTNVNGEFSLTLKNANSMIEVSYIGYEKQSLSHTGNGLIKVVLNENAKAL
                     DEVVVTALGIKREKKSLGYAIQEVKGDDLTKVKSNNVMNSLAGRVAGLKVSTTGTGEW
                     GTTNILLRGATSLSGNNQPLIVVDGVPVSGDSRGTGSENGGIDYGSGLSDINPDDIES
                     ISMLKGGNAAALYGSRAAAGVIMITTKKGIVKKTLGVKYSYDLGISTLNVMPDLQNLY
                     GRQTEIVDGVEQEVLKGTNWADSKLDGRMVKKWNGDIAPFTAQPNNIRDFFQTGIRNT
                     HSIEMDGGSDIAQARLSTMVSNSKGIIPNSELKKYSFSLSGNAKLGKKLTISGRATYL
                     IQNTQNRPNLADSPDNPMYSFLRMPRNIQLDDMRDYEDVNGFPRLWDGLAKSYTDDIS
                     KNQNPFWSVYKNINKDNRYRLMANVSAKYEFTKNLDLLVRAGTDYILDDNETRVAKRT
                     AYENNETRSKYAQNWGKSFENNFDLLLSYKNNFMDNKFSVVATGGGNFMHTWGNSIGF
                     YALALSLDDLYTVGNAKNVTSSQGFSQKQVQSLYGTVSLGYNSYLFLDLTAREDWNSS
                     LYSNINPSASVLNYFYPSASLSALFTDAFPAIKSNFLSYLKVRLAYARVGNGTSPYRT
                     SYTYSIGPGHLGQQYAYKPNVKPFVTLKPEMMNSSEVGLEANFFQNLLKLDATYYLNR
                     TSNQIIAQRIPSPSGYTTKLMNAGLVQNQGVELQLTTNIFNKKDFHWDVFVNFSRNRD
                     RIVELLDPNKKDRRIIASAGTLSIHAKAGGRYGEIYGTKYLRNDKGERIIGSNGLPML
                     AEDSIGSRTDNYLGNIQNDFLGGLGTNLSHKNFNFSALIDVRMGGKLYSFTEKITTEY
                     GNAPWTTTGREEWAASEIARIAAGKSPVEWLASRNQRGYLPQGVIQLPDGSYTPNRNL
                     YVSPQSYWINAAGVDEEFLHDASFVKFKELRLGYKLPKKWLKPLTINNLEIAAVGRNL
                     FYIYKAAPKGLDPEASFNGMGFEYSSIPSTREWGASMSVSF"
     gene            complement(17032..>18600)
                     /locus_tag="PLM04_04720"
     CDS             complement(17032..>18600)
                     /locus_tag="PLM04_04720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HON02412.1"
                     /translation="YFTAVFSKDMNFTKVVEPVFRPRSNDSIDVQKAVLSFETKKNEA
                     VEVKVAISYVSCKNAANNLIAELPNWNFKETLAQAQAKWNTELSKIRIKTNDEKVRTM
                     FYTSLFRTMISPSDFCDVNGDYRGADGKVYKANGFVPHTIFSLWDTYRTFHPLQTIIN
                     QKRTADWVNSLLDITEKQGSLPIWHLVGNETGTMVGVHSIPVIVDAVLKNIENIDAER
                     AYNLVRGFEQRNDRGLKFVREQGWIPADKENWSVSKGLEYAIDDYAIALLAKHLGKED
                     DYQLFSKRAKYYANYFDKELGFMRGKLADGTFRKGYNPAFSLHEEADYVEGNGWQYTF
                     LVPHDVEGLIELFGSKQKFTDKLDRLFTVSSELNEGASIDISGLIGQYAHGNEPSHST
                     IYLYQYVDRADKTAERVRQVLEEMYKPTPAGLCGNDDCGQMSAWYVMSALGIYPMNPV
                     NGEYVFGSPIVHNAVIQLENGNKFIIEAKNNSEKNKYIQSVKINGKMVYGKAISFNQL
                     MNGGKLEFRMSDKK"
     assembly_gap    18601..18700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18701..19055)
                     /locus_tag="PLM04_04725"
     CDS             complement(<18701..19055)
                     /locus_tag="PLM04_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HON02413.1"
                     /translation="MISKTKILTVILLITCHLQAVDFTRYVDPMIGSDYHGNVFVGAS
                     VPFGMVQLGPTNMSKGWHWCSGYHYSDSTIIGFAHTHLNGTGIGDLGDILFMPTTGTP
                     TTFKGTFDDLSKGYVS"
     gene            complement(19052..20851)
                     /locus_tag="PLM04_04730"
     CDS             complement(19052..20851)
                     /locus_tag="PLM04_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON02414.1"
                     /translation="MDVLVVGGGTSGTAAGIQAARMGALTLIVEETPWLGGMLTSAGV
                     SAMDGNHQLPSGIFGEFIDSLVAHYGSADALKTGWVSNVLFEPKVGNAIFQKMTKTEK
                     KLTVWQNSKFISATKIKHGWKVKVSKGGVIQSVTTKIMIDATELGDVAKHCGVKYDIG
                     MEARSVCGEDIAPEQSNDIIQDLTYVMILKDYGFDKTITKPASYDASLFYCTAKSSNC
                     TNPKPGQTLWPKEKMMTYGKLPNGKYMINWPIEGNDFYVNMLEMNDAERTVALEKAKN
                     FSLCYLYYLQTELGFSTYGLDEEQFPTADHFPLIPYHRESRRIHGLVRFNVNHVVKPF
                     SQPEALYRTGIAVGDYPIDHHHNRYPEWNKLPDLHFYPIPSYNVPMGALIPKDVDRLI
                     VAEKSISVSNLLNGSTRLQPVVLQIGQAAGALAALSVKQKKTISSVSVRDVQSELLNA
                     DCYLLPYLDLPKTDVHFKAVQRIGSTGILKGVGMNVGWSNETWFQCDSLIDVAELKEG
                     LNDFEPAFKWNSKSNELSIKEAIELVASLATFYKIKNADFGTISSNWQKMQLNNYNAE
                     RKITRKEFAVLLDTYIQPFSLKDVDMKGSYIKR"
     gene            complement(20935..22260)
                     /locus_tag="PLM04_04735"
     CDS             complement(20935..22260)
                     /locus_tag="PLM04_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON02415.1"
                     /translation="MKKNLLLLLSCCSVLATAQVSFTRVWERSDSAYTETGLEANKKP
                     LYFDDMNTRGIAVGNINSNAGMVERVFLVSRNVLGNVVRVLDVTTGADVKSLDMTNVT
                     GGGFIISDAGVTEDGVLLVSNCTHSSSGPFKIYKWDNEDAAPSVAISYPSGSARYGDK
                     ITVTGKYSDGTARIYAIAGGVLSNYKVQYFGMEEVNGTWQFKQTPDELNTSINAVSSN
                     FHQINPGPDGGYYFRSVGLSNFQQIKSDFTANIGNTPFPTTMPQGGTAPVFIKTVGGF
                     DYACYLQYGSHATYAAPANNRIQVIKIDRLMGITSATVLTSSPSFGKLAPGNGAGHVV
                     VKHQTNGDVDIFALSTQNGVVRYRLSGAEIGTDIRKSVDNLIGIKAFSGKISIEGINP
                     SSIELFNTLGQKIKSISNSNELQTSGLKGIYIVQVKAGGKTIKTEKISL"
BASE COUNT         6573 a         4685 c         3908 g         7107 t
ORIGIN      
        1 caatagtttt cacaacttta tactttcctc gaaacttctg tatcacttgt acagattgac
       61 ttccagatcg attttttact tttcggataa acataataat aattttgcaa cacccaaaat
      121 taaaatatta ttattgatat tcaaatactt ataagggtgt tgcaaaaaaa tgcggaaaac
      181 aggaaaaacc atgagagaaa gataagtgaa tatctaaaaa ctctcatggt catgtaacaa
      241 actaaacaac aatcattttt taatatactt gtatactttt acaccatgtg gaggaatctt
      301 tacaaatgtt gggtacttat cagtttttat ttctttatgt tcccataaat ctctaacaat
      361 ataattctga agttgataag gatcttgtgt ccaataatca tcatttgact taagcagaaa
      421 agcaaaatcc accataacct cttttatttc atcacttcta tttagtaaac aaacagcttt
      481 ttcatcatta gatagttttt ttatccaaat ctcataatct ccattatctc taaagcaaaa
      541 agcttgttta cctaacttat cctgatcgat agcaattaat tctttgttcg ttaatatctc
      601 taaagtttcc ttagacatat ctgacagatc attgcctgcc atcaaaggag cagccatcat
      661 acaccaaaga gaaaaatgag ttttatattc ttctatagtc atacctccat ttccaacttc
      721 taacatatcc ggatcattcc aatgattcgg tccagaatag ggtccaagat ctctctgctt
      781 atctacaata tctatcattg catcccaacg gtcaacaata tctcctgtag tgcgccaaag
      841 atgacctact tcttgagccc actcccatgg tcgtgatttt ccccattcac aaatactaaa
      901 aacgattggt cgacctgcgg cataaagggc atcacgcata atagaatagg aagctttagc
      961 atcttgtgtc ccggtattgc accaatcata ttttaaataa tccactcccc aacttgcata
     1021 agtttttgca tcctgaaatt catatcctct acttcccggt cgtccctgac aagtttttat
     1081 tccagcatcc gaataaatac caaatttcaa gccttttgaa tgaatataat cagctaaata
     1141 cttaattcca tgagggaaac gttgagtatc atgttgtata ttgccatttt catctcttga
     1201 aacttgccaa cagtcatcaa ccactatata ttcataacct gcatctttca ttcccgaact
     1261 tatcatggca tcagccattt tcataatcaa ctcctcatta acatcgcagt taaatttatt
     1321 ccaactattc caacccatag ggggagtgtt tgctaaacca tcaaattttt gtgcaattcc
     1381 atttaaacaa ataaacgtag tgaaaattaa caaaaaaagt tttttcattt ttttaaattt
     1441 atgttaaatt ttattcattt attaatcgag tcttaaatag cttcttgctg tatcataatg
     1501 caaatttcta tcaaaaagat gatctttctt tattttcttg taattaaatg cagctgctgg
     1561 aagcattaca ttatttttgc ctgtaattct tttgtataaa ttttactatt gtttatgttt
     1621 tccaaaacca ttttcttctg acggtgaaaa cagttactgc catcatgccg gatgtaataa
     1681 taactccttt cagtattccg aaccagggcg ttatttcgtg taaagtatta atatgcggca
     1741 ttaaacctgt aaaaactaat attggaacca caaaaaaagt acctgctaca taagccacca
     1801 tcgggttttg tccgcattcg gaaatgtagc ggaataactt cttattttca aaataatcga
     1861 ttacaaaaga gaaaaacagc agtgtgaaaa ttgccaaacc acttgtaacg aaaaaataac
     1921 tcatggtagc gctgtctttt cgtattccgc cttcgaatgc ttcgaaagcc gttccgagca
     1981 taagccaaaa aattccccat ttgtacaaac tgaaatacaa acggctaagc gaatgggttg
     2041 gtttgcgcat taaaatatat cctgcaatgc taaatgcaat atttagaatt aaatttaaca
     2101 gcaaaaatct tccgaaaaga caaatcagat tgctcagtac aaatgcaacc atgagcagaa
     2161 gcgatgctcc cattttttgt ttcgatggca atattgattt ttcattgtcc ttatcagtaa
     2221 accggcgaac cagcaaatcg ccggcaatgg ttccgggaat aacgataaac aggtatttca
     2281 gaaaatccat gcgatacagc caacctcccg gattgtaaac gattgttgtg attgagtcgg
     2341 gtaaaagctg cattggatta aaattccaca acacctgatt ccacgaacca tcgacacccg
     2401 atgtaagccg gagcgcaaat agaaatgcaa ggatacctaa ccgaagaagc agatttgtgc
     2461 gtgtcaaaat ccatgcaagc gaaccgaata aagccatatt ggcgagcacc agaataataa
     2521 tatcattgtg cgaaagtttg aaacctttac tgaaaacttc gggttgcgag ctgacattga
     2581 aggcaattaa tcccatcaga aaggcgattc ctaaccagtt cagccacgtg ttttttttct
     2641 ccgaaacgtt aggaaatcgg acaaaactga taaaaaacag accaaatgcc aatattgcca
     2701 aagcgaaatt ccagcctcca cccagccggt atggactgca atgataaatt gcaatggcaa
     2761 atagcaccaa taaaaaaaca cgtttcagaa ttgcaggaaa aagtgaagta actgtcgctc
     2821 ctttatctaa ttttcggcct aaagccagcg gaaaagccgc tcccatagca aaaaggaaga
     2881 aaggaaaaac caaatcgacc catgtaatgc caaaaatcgt tggatcgaaa ttaaaattgg
     2941 aacgcggtcc aacctgtgcg tgatacatcc aggcaggtaa agtaggggca acagtacccg
     3001 aaagtaccat gccgataata gctaaacccc gaagggcatc gagtgcggag gaacgattat
     3061 tcattaaaaa aatacttgca atttaactat ttaccattta ctatttttga tgtcaaattt
     3121 acattttgtc cttttatgtt caaaataaag atcccttttt gtgcaacaga aatagtttta
     3181 aaatcattgg acatggtcat attccagttt tcgcttaaaa tcagttttcc cgccaaatca
     3241 aatacgcgaa tctgaaccag ctgcgcactt tcgagttgca gattcagttg cagccgattg
     3301 ttttgatcgc tgaaaaccaa cagcttttgt ccgttatttt ctactgccgg cacagtggtt
     3361 gataagccaa gcgacttccg caagagatat gtgaattttt cgggcgtaac aaattcaaca
     3421 tcagcgccaa tgcttgtatt atttttaatg tcatccatca atgttttaag gcgtggtaat
     3481 gtaacatcat ttgtagccaa tgtagcattg tcgaaacgaa atcgttggta accggccatc
     3541 agaaaacggg gctttgtttt gccactcagt ttgcttacaa tccggtcgcg tacggtagtg
     3601 aggttcgaaa aatcggcata aaaagtatcg tcgtttacat gattatacag caaaccaccg
     3661 taattggtat aggctttttg cgtcatcagt gcagggtcaa acaacatggt catttgtgca
     3721 ttggtttgct cggcaaacaa acctaaattg ctgaaattaa aattattgga aatagtaatt
     3781 cctctatatt gagaagaacc tgctccgttg taatgcttat acgcatacac cgaatttaca
     3841 ttgaatttat ccatcaatgc aatagttttt gcttttgcat cgggcaaata cgaagtcgga
     3901 aacacatcgg gataagcata acccagcggc gtggacacgg ccataaaacc atcgttagca
     3961 gtggccgttc ggtaataata ctgcgctaca aacggaaatt cttcgaacat ttgtaaatta
     4021 aaaccccagg caacaggcac ttgtccgcgg gtagtcgatg cccatgcacc accctgaaaa
     4081 ccgatattcc aattgcctgc atcaccctcg gaactgatga aaagcacata gtatttatct
     4141 tccaacaaaa ctaattcggg attaatatca gccggacgtt gataactgaa atttgcatcg
     4201 accggaaaca catgatgcca cgacaaattg ctcaataacg tttcgtgaaa acttccaccc
     4261 caagccgaaa tccattgcac aagcggctcg ggacgcatcc agccatatac atgaaaaatt
     4321 tcattcggtc gtttggtttt caaataattc ataacccgtt ctattttagc tgctttttcg
     4381 tcgctgagcg aggtaaaacg taaatcctcg gttccggcca gattgagttg aaacatacgt
     4441 tgatttacag cccagtcggt tatttcccga aggtaaaatt tcgatgtatt gcaacgtggc
     4501 aacagattat cgagcgccca gtcaagccag gcaaaatagc gttgttcctg tgttaaagcc
     4561 gtgttattcc aactgtgtga ggttagttcc agttttgctg atactggtac ggtttgttgt
     4621 ccattaaaat aatcgggcaa atcaaccgaa gttggtttat ttatatcaat gattgtgttg
     4681 gttgatggaa ccggaatgca gtcggtaagt ccgccaagca tggcagccgc ttcagcttgc
     4741 catcgaaacg attgcccact gaagttgctg taagtcagcg tggcatcgta ggtaatggct
     4801 ccctttatgt agcttttgaa atactgaacg agagcattaa tattggtaat tacggtaaac
     4861 tgaacaccac caacggtttt gtattcattt tcccacaatt cgtcagtttg gttaaaactc
     4921 catgtctcgt acacatttcg caggtacaaa cgaggtgcgt cacggtttac aatcccggca
     4981 agcgtgagtg caattaattt atcctcagat aactgacccg aaccatcgta aaccactgca
     5041 tttatggcat ccgcattaaa agcaatataa aatgagataa gaagtaaagt gatttttttc
     5101 atttttttaa acaaagagca aaaaatttaa taataaaaat acaataaaca aaaacatgaa
     5161 tcccgaacga cgatctattt tttctttaca aaagaaacaa ctgttgccac accgcgttca
     5221 gttccatccg aaggtttgtt gagtaatttt ccgcctgcaa taatagcaga agaaccgcca
     5281 ccatcaagat taagtgcatt tacacaacca atacctttca taatttgctc caattcaatc
     5341 aaggtagctc catcactatg cgtttgtctg ccatcgcaaa caaaaagaac aattttatta
     5401 tcggcagtgc ttccaattac tgttcgtggc ggacgagctg ttgtggagaa aatatcagtt
     5461 tgtaaaagtt cataatttgt catgtatttt cccgaagttg tagttgtaaa atcgaaaaca
     5521 atttttccat ctttcagcaa aacaggagca ccaccaatgg cagaataagg attccagttc
     5581 tgtgactggg cagggaaact agccgacaat gatggttgcg gagtttcacc ctccacattc
     5641 ggacttggaa tatcataagc aaaattgccc accgcccatt tgatagatgg agtttgattt
     5701 tgagaaacgc caaatgcacc acgcgtaaca gcataggaat ggcttccacg tgtcaatgtc
     5761 gaaatattat ccgatttttt tgatgttctg tctacagcca gactataaga gttgacagtt
     5821 gaaccactgt atccaaaata gccggcattg gtcattacat atgttgtttc attggtagca
     5881 gcagcaataa actgacttgg tttttgaggc gaagtggata atgttgtttt caattcaata
     5941 attccggtgc tcatatctgc aatggcatac caggcactga aaggccttcc gtttagtgat
     6001 gaagttgtgc tataaacatt tatttcagta ggtaattctg catttataga tgtaattggt
     6061 gtccaggata acgcaattgc agggagttca ggggacaagg ttgcctgaat ttccggacta
     6121 taagtagttc ctttcgaatt tgtggcataa gctcgtatat agtaatttgt atttttgtct
     6181 aaattcaccg catttccaaa aaatgtttct cctgatttta cttttacatg ccacactgac
     6241 ttggagtcag ttatggtagg atttggtgat ttactccaac acaaaccggc attacaaggt
     6301 tctccaccat cggaagtaag agtaaaataa gctgatattt ttaaataaga aactatcggt
     6361 gaagcaattg taattatggg cgttttcagt atcattagtg agcgttccac accaaccgat
     6421 gtggcactcc ccgcataagc ataaagttta taagtgttca gtcctacggc cggatctgaa
     6481 tctgtaacag aagttgtgtt tgcatcaagt gttttccacg cttggtaaat ttctgtgcca
     6541 atcttgcgtt ctattttgta accatcaatg ttttctgtta gagcgtccca tgttagattt
     6601 acagtattat gggcggctac aactgtgaaa tttgtaggca tagaaacagg aacgtgctga
     6661 taataaaccg agacagtatc ggacacacgt tgtttgcaaa atccataaac tttgtataaa
     6721 tacgttgcct cctgtagtcc ctcatcgatc cattcggtcg tatttgctcc aacagttgct
     6781 tttacgggta ctgacgttcc agtaacccaa cgttcgatag tgaaaccatc ttcaccattg
     6841 ctgttgtccg accagcttat ttttaccgag gttgtggtta ttttttcgac aaccaattct
     6901 gttggagcgg ggaatggtgc ctttggatta ctcaaatcag gctcgttgca agagtaaaat
     6961 gttgaaaaca tcggaataag gagaaaaagc ttgtacctta tttttgttgg actcacaaac
     7021 atggtttatt gtatagtaaa ttaaaatgga aaaattgcct tgccgggata caaagcttta
     7081 tatacttttt gttttgatgg aatagcttcg taaaaccacg tggactcacc ataaacacct
     7141 ttagtacggt tatattgaat taattgtgcc aaaaattcat cagagacaat tgtatttcca
     7201 ttgcgtacca acatgccagg aacatacttt ttacgtaaat cagctgtgtt aaaaacattt
     7261 tgtacatcgc tataaacaag ggttgtatac gaatcaactc cctgcgattc gttacgatac
     7321 aattgaggcg aaatccaatc agcgacacct cttctgagcc attcaggcca gtcctgcaaa
     7381 tagttatccc tactccagcc acgtgtactt ggagcccaag taaccagaca agtagaacgg
     7441 gcagctttca ccgcattata aagactaaca gcataatcgc tcattttatt gactttccaa
     7501 ttaaaaaaaa tggtttctgt ggggttggat ggaacatctc ttcctgtttc agttttataa
     7561 agttgtttgg tataatcgtc gtatccagca tgtgccggaa gagctggcag gcggtcgtcg
     7621 ccctgaatgc catccaattc tggatatttt gtaacgcatt ccacaatcat atcggtcata
     7681 aattgctgca cttcgtgatg gaacccgttg agccagtaaa aactgttttt aaccgtttgt
     7741 gtcccatcct gttgtttact cacccagtcc gggtgtttat ctgagatagg tgaatcctga
     7801 cctgaataat gtgaagcaaa tccatattcg aaccaggcaa ttacctttat atttttagcg
     7861 tgagcttttt caatcagttt ttctaataca tcgccccacg agcaaccaga aaatactgtt
     7921 tgtgcaggtg ttgttcccaa cacggtagcc agcgtttgac tggggaaaag agtttgactt
     7981 ttattgtatg tacaaacaca tatcgtgtta aaattgagtt cggccagttt atctaccata
     8041 tccgtaagag ctgattctga aaataaaact gagcttgcta catttgtaac ccaaacagct
     8101 cgtacgcctg tttcctgtgg ttgaatatta gctgtaatag tatatgtttt tgagagattc
     8161 ccaattgata atttccacaa aaacggttgg cttaaatcga ctgttgaacc cgaagcgata
     8221 ttggatgtta atccattgtc tgctaacaga taagtcaact tcattgccga aacatttgtt
     8281 ccaaaaggaa cagtaaatgt aatattgtcg ccattgatag ttccggttac aaaattatcg
     8341 ggaagtttga cgttattgac tctgaaagta ggttgtgagt ataccagaac tatgtaactt
     8401 ttttttgtac ccaactcgga agttacagta taaaatacag gttttgaaaa atcctgttta
     8461 acacctgaag caggattaat ggttgctttt actgaaactt caatcactgg tgtaagtgaa
     8521 gttaaatcag tgtttgttgg caccataatc aaaactgtat cgtttgaaat ataagcctca
     8581 ttggcttgtg aaatttcctg aaatttgaaa ctaattattt catttcctcc ctccaatgat
     8641 tgtttgcacg aaagtaataa aatacaagtg aagaataaaa aaaatttttt catcagaata
     8701 taatttacta aagtttcgca cttcgtttta atgatttgaa agacaggaaa taaagaagtg
     8761 cgattaagtc atttcctgtc tgattatcaa tatattaata attccatttt cgtcaaactg
     8821 cttccctgag cggaggagta agcatttttg aaaccaattc cgccgtcttg ggacaattca
     8881 tgatcaactc aaacaattcg tcaatatctt tctcaaatgc ttcacatatt tcctgcaaca
     8941 actccaaaaa taatccccaa agacgttcgt caagcgtttg ctgcaaacat tccgcattca
     9001 ttaccctgaa gagttctccc atcgattcat aatgttcata ccgatatcga aaagacagta
     9061 aaatataggc tatcatgctt atcgtggtat cggctatctg tgcatcgaaa tcattggatt
     9121 gacatcctcc cagattcaac aattgcttcg tttctttgaa aaatacttct atcgaccacc
     9181 gtatctggta gatttccatc agttgcacaa aactcaactt cgtatcggtg gttaaaaata
     9241 ctttccattt ccctctcttg ccttgacggg taaagaataa cgttaccgca ataccatcca
     9301 acattacatc tgcacgcttg tagtgcagtt tcatctttct gcaacgggtt attttatcaa
     9361 ttgaactttg tatctcccgg taattcatct gcttgccctg atacagaaat tttgctttta
     9421 ctatcctgta catgcctatc aggtgaatat gctttgaacc cactgccgta attaatgcct
     9481 gacaggtaaa ccaactgtcg cacaaaacat aatcgagggg aatgtgataa aatacagccc
     9541 ggtaaaaaat ctcaagcatc atttctatct tgctcttatc caattctttc tcccgttctc
     9601 tccctgaaag ctccttcaaa cgctttttgc tgtattgatg gcgtaattcc tttcggctca
     9661 ttccgtaagg cttctctgcc cgtttccctt tctctcttga aagtgaaaaa tccaatggaa
     9721 tcatcgactt tccgtcccaa tacaatccta acaacagctt gtatcccaat ttgaaagtgt
     9781 ttgttacatg atcgtaaact ttcccgatct tttccatctt tttccctgtc ttttcaagca
     9841 gggtgtcatc aaatatgaga catcgtacct tacatgatac ttctttctcc tgtccggtaa
     9901 tgccgataaa cttacacgct gtctgccata acagcttccg ccaattgatc cctgcattgt
     9961 tcttcaaacg gtacaatatg ttctttccca tcgtaatgcc tttttccttt agcaggggca
    10021 gcgaagatac tgccgttttt cgtcccggcg tcacggcata aatcattgac gacaatacca
    10081 actgaaaact gtaaccactc cttttcaaat gcttgaattc cgaaaatcct tctgtcagtt
    10141 tcaacgcctt gaaacctttc aaaatattta tactctccaa tttattttca ttgaaatctg
    10201 ttccaatttc agaaattttt tccttaattt tgtccggtag catatttttt tctgttttgt
    10261 aattatacaa ctaatatact catttttaat gatatattag ataaaatatg ctactttttt
    10321 ttacttcttt aactcttttt cctctttatt ttcagccctt tctgaaatga gaaactttta
    10381 taatttattt tttaaaggtc tgatggaacc ctcatgtctg aatattatat tcattcgtgt
    10441 ttgtgtatgc aatacatgtg ggtttcatat aaagtattat tgagaacaag gcccacacgt
    10501 taacgcgtgg actttgttgg aaatagaatt aattgcaaat ttttcaatta ctaattaaaa
    10561 gtcagttctt tctttttgct gaatttagtt acaacaatac cacatccggt acccaaagcc
    10621 acaactttga aacccagacc atcagcatca agaattgcat ctatacatcc ggttgcatct
    10681 ccgtttttta atgttccatc agcttcaatc ttttcaggct gcggagttag ccaccagtta
    10741 ttgctcattt caacgctgtt tgtggtagtt gtataatcat acagtcttgc atttacgctg
    10801 tgaggagttg cataagcctc aacatttaag ggaatgtaat attttacatt attaaactca
    10861 agataagttg ctattccttt aggtgttgct acagctgtag caaacatata agtttgttta
    10921 gtaattccat tcataactcc aacttcagat cctgcacaag cagcatttac caaaatatcg
    10981 ctggttgctg tagccccaag cggaacaatt gaagcccaca attcgaagcg cgacatagaa
    11041 ccacccggtg tgtaatgaat aaactcggga ctcgaagcgg caactccgtc agttactttc
    11101 caacgaagaa tgtccagtgt ataatttgca taggtataaa tataagcttg agtttttaaa
    11161 tcaccaataa ccgaaatacg tcgtccaaca tggttgccag cccagcttgg tggagtcccc
    11221 accggtgtca aatccgacac attccatttg atcagctcac gtggagcggc gtctacgtta
    11281 tcccaacacc atactttaaa gtcaccacct ggagctgcca aattacatcc gataaaactg
    11341 cctttacggt ctccagccat tgcacgaatt tgtttaatcg tatcgccggg agctactact
    11401 aatttagcat atcccgtttc ggagccatca agcaatgaaa cttttaccag ttttaatgtt
    11461 ccaccatata atccccctat ttgttgaaca atatgagttg gggttaaagc aaatgttttt
    11521 tcattattat cagtaaactt atctgtaaaa gctgttccat ttttacgcca gtattcagaa
    11581 attacgccat accctttctg agctgtttca aaaaaacgaa ctttgtatgc cgttttcgaa
    11641 acaccatctt cagccgtaac ggtgtaatac accggagttg ctgaattaaa tttaacaggt
    11701 gttcctgatg ccggtgaaac tgttgcccct tccgatactg taagtgttgc ggcgttgctg
    11761 ttcaaaagtc gatttatcgt atctggcttt aaaccaaatg gtaggttaat ctgaatttct
    11821 tctcctacaa tttcgacaga tatactggct ttaatagttt gatcagctaa agcatccaac
    11881 gaactaagat ctagggcaaa tgatttaatt tcagagtcag ttttcagaac cacttctggt
    11941 tcatttttgc atgatgcaag tgataaagca acaaaaagca caatagcaat tttgttaaat
    12001 aaacggtttg ttttcattgt aactaaattt aaattgttta agtaaattat attttgaaaa
    12061 ttatgaattg ttaattatta attatttacg tcccaccaca ttctgctcaa ctgataatcg
    12121 ccacccatac ggtttgccgc ttcggttacg tttgctttgt taagcaggtg ttcatcgctc
    12181 ggatatttcc aacgtctagg taattccatt tcgtcaggaa cagatgaagt atactcaatt
    12241 ggtaaaattt taggataacc tgtgcgacgt aagtcagcgt aagcttcgaa gccgtcagta
    12301 taatttccca aatatttttg taaaataatt tgttctaatt tttcagcttt tgtgccattt
    12361 gacttaaatt tagcagcccc agctaaatat ttatttattt cactgtcagg cacttcatat
    12421 aattgcatag ctgctcttac accatcatta taatgttctt cagcagtttt aggagttgtc
    12481 cagccattca ggtttccttc ggctaataaa aaacaaattt cggggtaaac aaaaatattg
    12541 gttggataat tgtcctgacg caaatactga ctcaaatcag aatattcacc aagtttactt
    12601 tgcaatgtag ttagtgattc gatgctttga ccattccgta atcccctgta ttttatactt
    12661 ctacttattt cagaacttct tgaaggcaaa acacgggcaa caatcgcttc tatttcgtcg
    12721 gtaaaataac gtccctgggc aaaaatattg ataaaagacc cgccttctgc agctgcgtaa
    12781 cttacttttg cttgctcggc atacgacaat gaattataaa tagtagaatt ttgttcaata
    12841 tccttacata tttgtttcag agcattcagg taattttttg ttggttctgc aaaattgaat
    12901 attcgaggat catctagctc gttaagagtt ttaaccataa attcgctaat gcggtaccag
    12961 tcgaacgcgt tagccattac ttttatcgga ttgtcgctgt gtattccctg aggtccggcc
    13021 ggatcagttt taagccggca agattgcaga ttattactca ttaaccctcc tgcgtctgct
    13081 aatgtttcat caaaccatgt tttagcaagt gtgggattaa ctttcgaaat tcggattgcc
    13141 attcgtaagc gtactgaatt gccaaatctt ttccatgagc tgtagtcgcc attataaaat
    13201 aaatcggcac tgcccattcc atttcctttt gttgcatcta ttttttgtac acttgtttta
    13261 agcgaattat aaattcctat atatatatct tcctgtttgt cgtactttgg ataatctatc
    13321 aaatcattga aagcctcgct gtaaggtata tcaccaaaaa ggtcagtcaa ttgcaaaaac
    13381 aaataagatt tccagatatt acaaatggct tctttatttt cccaaccttt ctgttttgca
    13441 attttttcgg cttcagccac aaacgaaaga tatgtttttg aggcatcgcc agcataagct
    13501 gcactccaca aagtcccatt ccaggctgcg ttaaattgat acctgtcggt acaatgttct
    13561 ttagtgtaat ttgtattggc gtacatttgt gtatggaaac aagccatacc acgggtgtaa
    13621 taataaacgc ggtcgtccat tgcaccacga cgttgagaag tggtgaagag gtaagccgga
    13681 tcaatcgttg tagatgcatt tgggttcgtg ttaatttcgg caaagcggtc ggtacatgcc
    13741 gaaaaaacaa ctgctacaag taaaataaat attgtatttt tcattttcta ttaaatttat
    13801 atcgttgtta tttaaaagct tacactcatg cttgctcccc attcgcgggt tgatggtatg
    13861 gaactatatt caaaccccat tccgttaaat gaagcttctg ggtccaaacc ttttggagct
    13921 gctttgtaaa tgtagaataa attccggcct accgcagcta tttccaggtt attaattgtt
    13981 agtggcttta accatttttt tggtaattta tacccaagtc ttagctcttt aaatttcaca
    14041 aatgatgcgt cgtgcaagaa ttcttcatct acaccggctg cattaatcca gtatgattgt
    14101 ggtgaaacgt acaaattacg gtttggagtg tagcttccat caggcaattg aatcactcct
    14161 tgcggtaaat atccacgctg attacgcgag gctaaccatt caacaggaga tttgccggca
    14221 gcaatacggg caatttccga agctgcccat tcttcacgtc cagttgttgt ccagggtgca
    14281 tttccatatt ctgtagtaat tttttctgta aaagaatata actttcctcc catacggaca
    14341 tcaattaatg cactgaaatt aaaattttta tgacttaaat ttgtccctaa acctcctaaa
    14401 aagtcatttt gaatattccc aaggtaattg tctgtacgcg aaccaatcga atcttcggca
    14461 agcattggca aaccgttcga accaattata cgttctcctt tgtcgttgcg caggtatttt
    14521 gttccgtaaa tttcaccata acgcccacca gcctttgcat gaattgacaa cgttcctgcc
    14581 gaagcaatta tacggcggtc tttcttattt ggatcaagca gttcgacaat tctatcgcgg
    14641 ttgcgggaga aattaacaaa cacatcccaa tgaaaatctt ttttattaaa gatatttgta
    14701 gtcaactgta gctcaactcc ctgattttga accaaacctg cattcatcaa cttggtagta
    14761 taacccgaag gtgatggaat gcgttgtgca attatctggt tcgatgtacg attcagataa
    14821 taagtagcat caagcttcag aagattttga aagaaatttg cttctaaacc gacttccgaa
    14881 ctattcatca tttcgggttt aagtgtaaca aatggtttta cattgggttt ataggcatat
    14941 tgctgaccaa gatgtccggg accaatggaa taagtataag aggtacgata aggacttgtg
    15001 ccgttaccaa cccttgcata agctaatctg accttcagat aagataaaaa gttggattta
    15061 atagcaggga aagcgtcagt aaatagtgca gacaaacttg ctgatgggta aaaataattt
    15121 aacacggatg cagaaggatt tatgttcgaa tataaacttg aattccaatc ttcacgggcg
    15181 gtaagatcca aaaacaaata gctgttatat cctaaagaaa cagtcccgta tagcgattgt
    15241 acttgcttct gactaaatcc ttgtgaactt gtaacatttt ttgcattacc tactgtgtac
    15301 aaatcgtcaa gtgaaagggc caatgcataa aaaccaatac tgtttcccca tgtatgcata
    15361 aaattacctc caccggtcgc aactacagaa aatttattgt ccataaaatt atttttgtag
    15421 gagagcaata aatcaaagtt attttcaaat gattttcccc aattttgagc gtatttcgaa
    15481 cgggtttcgt tgttctcata agctgtacgt ttggcaaccc ttgtttcatt atcatccaga
    15541 atatagtctg ttccggctct gaccaacaaa tccaaattct ttgtaaattc atattttgcc
    15601 gatacatttg ccatcaaacg ataccgatta tctttgttaa tgtttttata gacagaccag
    15661 aatggattct gattttttga aatatcatcg gtgtaacttt tagctaatcc atcccacaac
    15721 cgcggaaatc cgttcacatc ttcatagtca cgcatatcgt cgagttgaat atttcgtggc
    15781 atacgtaaaa aactatacat cggattgtca gggctgtccg ctaaatttgg tctgttttgt
    15841 gtattttgaa ttaagtaagt tgcacgtccg gaaatagtga gttttttacc gagttttgca
    15901 tttccactca gactgaaact atatttcttt aattcgctat tggggataat tcctttggag
    15961 ttacttacca tcgtcgataa tcgagcttgc gcaatatcgc taccgccatc catttcaata
    16021 gaatgtgtat ttcgaattcc ggtttgaaag aaatctctaa tattgttggg ttgagctgta
    16081 aatggagcaa tatcaccatt ccattttttt accatacgac catccagttt tgaatcagcc
    16141 cagtttgttc ctttcaacac ttcttgttct actccatcta caatttcagt ttgtcgaccg
    16201 tatagatttt gcaaatcagg cattacattt agcgttgaaa tgcccaaatc gtaactatac
    16261 tttacaccaa gtgttttttt tacaatacct tttttggtgg taatcattat tactccggcg
    16321 gccgcacgcg aaccataaag tgcagctgca tttcctcctt tcagcatcga aatgctttcg
    16381 atatcatcgg gattaatgtc tgacagacca ctaccgtagt ctataccgcc attttcactt
    16441 cctgtaccac gtgaatctcc acttacagga acaccgtcaa caacaataag tggctgatta
    16501 ttaccggata aagatgttgc accacgtaat aaaatattgg ttgttcccca ttctcccgta
    16561 cctgttgtgc tgacttttaa tccagcaaca cgtcctgcaa gcgaattcat tacattgttt
    16621 gatttaacct ttgtaaggtc gtcgccttta acttcctgaa tggcatatcc aagcgatttt
    16681 ttttcacgtt tgataccaag cgcagttaca acgacttcat ccaatgcttt tgcattttcg
    16741 ttgaggacaa cttttattaa tccattacct gtgtggctca atgactgttt ttcgtacccg
    16801 atataagata cttcaatcat cgaatttgca ttttttaaag taagcgaaaa ttctccattt
    16861 acattggtta ctgttccatt gtttgttcct ttttccaaaa cagttgcacc aataatggct
    16921 tcaccttttg cgtcggttac aattcctttt acaatttgtg catttatttg tccgaatgcg
    16981 aacaattgaa ataccagtac aattaaaagt tttacatttt ttttcatatc attatttttt
    17041 atcactcatt ctaaattcaa gctttcctcc gttcattaac tgattgaaag aaatggcttt
    17101 tccgtaaacc atttttccgt tgatttttac tgattgaatg tatttgtttt tttctgaatt
    17161 attcttggcc tcaatgataa atttatttcc gttttccaac tgaattacag cattatgcac
    17221 aataggacta ccaaatacat attctccatt cacgggattc atggggtaaa tgcccaaagc
    17281 agacattacg taccaggccg acatttgtcc acaatcgtcg ttgccgcaaa gtccagctgg
    17341 tgtgggtttg tacatttcct ccaatacctg acgtacgcgt tcggctgttt tatcggctct
    17401 gtcgacatac tgatacaggt aaatagtgct gtggctcggt tcgtttccgt gcgcgtactg
    17461 accaatcaat cctgaaatat caatcgaagc gccttcgttt agttccgaac ttactgtaaa
    17521 taacctgtct aacttatccg taaatttttg tttactacca aaaagttcta tcagtccttc
    17581 cacatcgtgt ggaaccaaaa acgtatattg ccagccgttt ccttctacat aatcggcttc
    17641 ttcgtgcaac gaaaacgcag gattgtatcc cttgcggaat gttccgtcag ctaattttcc
    17701 gcgcataaat cccaattctt tatcgaagta attagcgtaa tattttgccc tttttgaaaa
    17761 caattgataa tcatcttctt ttcccaaatg tttggccagc aatgcaatgg cataatcatc
    17821 aatagcatat tccagccctt ttgaaaccga ccagttttct ttatcggccg gaatccagcc
    17881 ctgttcgcga acaaacttca atcctctgtc gtttcgttgt tcaaaaccgc gaaccaaatt
    17941 atatgcacgt tcagcatcaa tgttttcaat atttttcaaa accgcatcta caatcaccgg
    18001 aatggaatga acccctacca tcgtacccgt ttcgtttcca actaaatgcc agattggcaa
    18061 actgccctgc ttttcggtaa tatccagcaa agaattaacc cagtcggctg ttcgcttctg
    18121 attgataata gtttgtaacg gatgaaaagt tcggtatgta tcccacaagg agaaaatggt
    18181 gtgtggaacg aaaccgtttg ctttgtacac ttttccgtca gctccccgat aatcaccatt
    18241 cacatcgcaa aagtcagaag gtgaaatcat ggtacgaaac agcgatgtgt aaaacatggt
    18301 acgcactttt tcgtcgtttg ttttaattcg gattttcgac aattcagtgt tccattttgc
    18361 ctgagcctga gctagtgttt ccttgaaatt ccagtttggc aattcagcta taaggttatt
    18421 agccgcattt ttgcaactta catacgatat agccactttt acttccaccg cttcattttt
    18481 tttagtttca aaacttaaaa ccgctttttg cacatcaata ctatcattcg aacgtggacg
    18541 aaaaacaggt tctaccactt ttgtaaaatt catgtctttt gaaaaaacag ccgtaaaata
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tagaaacata gcctttcgat
    18721 aaatcgtcga aagttccttt aaatgttgtg ggagttcctg tggttggcat aaacaaaata
    18781 tcgcccaaat cgccaatgcc agtaccgttc agatgcgtat gtgcgaaacc aatgatcgta
    18841 ctgtcggaat aatgataccc cgaacaccag tgccagcctt tgctcatatt tgtcggtccc
    18901 aattgcacca tgccaaacgg cacgcttgct cccacaaata cgtttccgtg atagtccgaa
    18961 ccaatcattg gatcaacata gcgcgtgaaa tcaacagcct gcaaatggca ggttatcagc
    19021 aaaataacag ttaatatttt tgtttttgaa atcatctttt tatataactt cctttcatat
    19081 ccacatcttt caatgaaaac ggttgaatat aggtatcgag cagaacagcg aattctttgc
    19141 gtgttatttt tcgttcggcg ttgtaattat tcagttgcat tttttgccag tttgaggaaa
    19201 ttgttccaaa atctgcattt ttgattttat aaaaagttgc caacgatgca actaattcaa
    19261 ttgcttcttt tattgaaagt tcgttacttt tcgaattcca tttgaaagcc ggttcaaaat
    19321 cgtttagccc ttcttttaac tccgcaacat caattaaact gtcgcactgg aaccatgttt
    19381 cgttgctcca gcccacattc attcccaccc ctttcaaaat tccggttgaa ccgatgcgtt
    19441 gtactgcttt aaagtggaca tcggttttag gtaaatccag atagggaagc aaataacaat
    19501 cagcattcag aagttcgctt tgtacatcgc gcaccgatac tgaactgatt gtttttttct
    19561 gttttaccga aagcgctgcc aatgctcctg ctgcctgacc aatttgcaat accactggtt
    19621 gcaagcgggt tgaaccgtta agtaaatttg aaacagaaat cgatttttcg gcaactatca
    19681 gtcgatcaac atcttttggt atcaaagcgc ccatcggtac attgtaggac ggaattggat
    19741 aaaaatgcag atcgggcaac ttgttccact ccggataacg gttgtggtga tggtcaatcg
    19801 gataatcgcc tacagctatg cctgttcggt aaagcgcttc gggctgacta aatggtttta
    19861 caacgtgatt cacgttaaat cttaccaaac catgaatacg ccgactctcg cgatgataag
    19921 gaatcaaagg aaaatgatcg gcggtcggaa attgttcttc gtctaatcca taagtggaaa
    19981 aaccaagttc cgtttgtaaa taatacaagt aacaaagcga aaagtttttt gctttttcga
    20041 gtgcaactgt acgttcggca tcattcattt ccagcatgtt aacataaaaa tcatttcctt
    20101 ctatcggcca gtttatcatg tatttaccat taggtaattt accataagtc atcatttttt
    20161 cttttggcca aagagtttgt ccgggtttag gattggtaca gtttgatgat tttgctgtgc
    20221 aataaaacaa tgatgcatcg taactcgcag gctttgtgat ggttttatca aagccataat
    20281 ctttcagaat cattacataa gtcaggtcct gaataatgtc gtttgactgc tcgggtgcaa
    20341 tatcttctcc acaaactgaa cgtgcttcca tcccaatatc gtattttacg ccacagtgtt
    20401 tggctacatc gcccaactcg gtagcgtcaa tcatgatttt tgtagttact gactgtatta
    20461 caccgccttt actcaccttt actttccatc catgtttaat ttttgttgcc gaaatgaact
    20521 ttgaattttg ccaaacagtt agttttttct cagtttttgt cattttttga aaaattgcat
    20581 ttcccacttt tggttcaaac aaaacattgc ttacccagcc tgttttcagg gcatcggcac
    20641 tgccgtaatg tgccaccaaa ctatctatga actcaccaaa aatgcccgaa ggaagttggt
    20701 ggtttccatc catcgccgac acacctgcag aagtaagcat tcctccaagc cagggtgttt
    20761 cttctacaat aagtgttaga gcgcccattc gagcagcctg gatgccggca gcagtaccac
    20821 ttgtaccgcc gcctacaacc agtacatcca ctttttgcgc aacagaagtt aaatgaaata
    20881 aaacaaaaat tatgaaaact aactttttaa gcataaacac gacacatgaa aattctacaa
    20941 actgatcttt tcggttttta ttgtttttcc tccagctttt acctgaacta tataaattcc
    21001 ttttaaacca cttgtttgca actcgttaga gtttgaaatc gattttattt tttggcctag
    21061 cgtgttgaat aattcgattg aagaaggatt gataccttca atgcttatct ttccgctaaa
    21121 tgcttttatt cctatcaaat tatcaacaga tttacgaatg tctgtcccaa tttcggctcc
    21181 tgaaagacga taacggacaa caccgttctg agtcgataaa gcgaaaatat ctacatcgcc
    21241 attggtttga tgtttcacca caacatgacc tgcaccatta cctggagcaa gtttcccaaa
    21301 tgaaggcgaa gatgttagaa cagttgcact ggttattccc attaaacgat caatttttat
    21361 cacctgaatt ctgttattag caggagcggc ataagttgca tgactgccgt attgcaggta
    21421 acaggcataa tcgaagccac ctaccgtttt aataaaaaca ggagcggttc caccttgagg
    21481 cattgttgtt gggaagggtg tatttccaat atttgcagta aaatctgatt taatctgttg
    21541 aaaattactt aatcctaccg aacggaaata ataaccacca tctggtcccg gatttatctg
    21601 gtgaaaattt gaactgacag cattaatgga tgtattcagt tcatcgggcg tttgtttaaa
    21661 ttgccatgtc ccatttactt cttccatgcc aaaatattga actttgtaat tagaaagtac
    21721 acctccagcg atcgcgtaaa tacgggcagt tccatcgcta tattttcccg taactgtaat
    21781 tttatcgcca taacgagccg aacccgatgg ataagaaata gcaacagatg gtgccgcatc
    21841 ttcattatcc catttgtaaa ttttaaatgg gccagaactg ctgtgtgtac aattcgagac
    21901 taataaaacg ccatcttcgg taactcctgc atcgcttata ataaaaccgc cacctgttac
    21961 attggtcata tctaatgatt ttacatcggc tcctgtggtt acatccaaaa cacgaaccac
    22021 attgccaagt acattacgac ttactaaaaa aacacgctca accatacccg cattactgtt
    22081 aatattgcca acagcaatgc cccttgtatt catgtcatca aaatagagag gttttttatt
    22141 tgcctccagc ccagtctctg tatatgcaga atcgctacgt tcccaaactc ttgtaaaaga
    22201 aacttgggct gttgctaaaa cggagcagca actgagcaac aaaagtagat tttttttcat
    22261 cagcatataa tttattttta atggtctgat ggaacccaca tgtctgaatt attatattca
    22321 ttcgtgtttg tgtatgcaat acatgtaggt ttcataaaat tagttttttt aac
//
LOCUS       DAOTAX010000030        21733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_1971_length_21733_cov_31.964895, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000030 DAOTAX010000000
VERSION     DAOTAX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21733
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_21733_cov_31.964895"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(612..1031)
                     /locus_tag="PLM04_04740"
     CDS             complement(612..1031)
                     /locus_tag="PLM04_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HON02416.1"
                     /translation="MYYSIAYSQQYLIQHSIKPSVQRTAVMDYLLNNRTHPTVDEIFS
                     VLSPSMPTLSKTTVYNTLKIFIERGAARMLTIDEKNARYDVDTTPHAHFICQQCGKVS
                     DVFDVRPEVFQLPLNDNFKVNSVEIFYYGVCNNCSSK"
     gene            complement(1205..2146)
                     /locus_tag="PLM04_04745"
     CDS             complement(1205..2146)
                     /locus_tag="PLM04_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HON02417.1"
                     /translation="MSKVSVIGAGNVGATCVNVLSFYQIADEVVMLDVKQGIAEGKAL
                     DINQTATLLHFNTRISGCTNDYEKTANSDVVVITSGVPRKPGMTREELVDVNAGIIKS
                     VVENVLKYSPNAILVIVSNPMDTMTYLTYKIAGLPRNRVIGMGGTLDTARFKCYLGKA
                     LNANQNEIEGFVIGAHGDTSMIPLVRYAAYKCRPVTDLLDKETLDKVVSDTMLGGATI
                     TSLLGTSAWYAPGAAAAFLVEAIIHDEKKIIPCSVYLEGEYGQNDICIGVPVLIGRNG
                     VEEILDYHLNSEEKELFIKSSESLRATNAMLKDLGVI"
     gene            2453..3592
                     /locus_tag="PLM04_04750"
     CDS             2453..3592
                     /locus_tag="PLM04_04750"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HON02418.1"
                     /translation="MKNYFYSLVIIILLIGCQNKNSFTVEGNISSAKGEKLYIEHMGL
                     LKTTSVDSVKLGKNGKFHFTIKRPEYPDFYRLRLNDKLIIFAVDSTEKITIKASKEDL
                     PYNYTLTGSEQSLKIQQLRKSVANIQNKINKFSSNLAPEEQNQLMKEIEKDIENHKRE
                     AQKLILENPRSTAAYYALYQKVNDFYLFSPYVKEDRSYCAAVATAYHTFMPNYTRSQN
                     LYATVMDAIKNERKAESQLRLEELFRTNSTGYINIVLPDKNGIQQKLSDLEGKIVLID
                     FSIYTARENVEHTFSLRELYNKYHERGFEIFQVSLDENRFLWEKSVQNIPWICVRDER
                     GPNTPYVLSYNISTIPASFLMDRQGNIIARDLDFKQLDKQIAKLL"
     gene            3668..4606
                     /gene="queG"
                     /locus_tag="PLM04_04755"
     CDS             3668..4606
                     /gene="queG"
                     /locus_tag="PLM04_04755"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HON02419.1"
                     /translation="MRNFIINTALSIGFDACGITKAETLSADASYLQSWLKAGCHASM
                     KYLERNFDKRVNPKLLVPGCESIIVVLMNYFPSEMQLSAAPQIAKYAYSKIDYHKVLK
                     TKLQQLEKAITEKFGKNSFNDEVQHIFVDSAPVLERRWAEKAGLGWIGKNKQLIHPGL
                     GSYVFIGILMMNAETEYDLPIKNRCGNCRKCIDSCPTQALSEEWLDANKCISYLTIEC
                     KTNIPEEFQGKLSNYAVGCDICADVCPWNRKWAQPNRHPELETASPIKKWNCKQWENL
                     NQEEFESAFSKSAIEYVGYSKLKENISILMEKFKKD"
     gene            complement(4630..7017)
                     /locus_tag="PLM04_04760"
     CDS             complement(4630..7017)
                     /locus_tag="PLM04_04760"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HON02420.1"
                     /translation="MENNIDSRVAANENEVIDLRQLLIKYLSKWHWFVISIVFCFIVA
                     FLYLKITHNKYQVQTTILLRNQNSTPELSNMTMFSNMGLLGVSKEVEDEIQVLSSKTL
                     VRQVIDSLNLGTEYYVKNGLRYDEVYPISPVQIFVSNNFNDTLKQKIILILSPTSKGY
                     DISFQYGEIIEKKYRVSDLSTPLSTPIGSIRFFVNGPLKSGQKIKIISYPLAALTNQL
                     AASISVATVNKNSNAISISTVSPTPHKAKVMLDKLVELYNLDAIFDKNMILTNTADFI
                     ESRLRLISSELLDVELNVENYKKTHGLTDISSEAQLYLETASEYEKKLADIETQLNLV
                     NYVESYINESKNQFNLIPANLGIKDPALQKLIEEYNVALLERMKILRSSNEQNPVITQ
                     MEQQINAIRSNIVKSIAGVKDGLVISKKDLMAYNNEFASRIKSVPTQERQFMEIKRQQ
                     QIKESLYVFLLQKREENALNLASTIPSARTIDAAYTTGSPISPKRMIIFLLALILGLG
                     LPILVMYLMEFFNNTVQDRKEFQKFVKAPFLGTIGLSKETENIVVKEGRTTPIVEMFR
                     LVRSNLQFMFSDIKSPVILVTSTISGEGKTFIAANLAMSFALMKKKVILVGVDIRNPM
                     LAEYFNLKKNNKGLTIFLADPNCRMDEIIQPSEVHPYLDVILAGAVPPNPAELLMSQR
                     LDELIRELKSRYDYIILDSAPIGIVSDTFQLNRLVDNTICVARLKYTPKEALHFINHT
                     YESKKLKNMATVLNGTNDVSHYGYGYKNEKKYKYRYRETTQNALEKRLNKLKKNK"
     gene            complement(7055..7840)
                     /locus_tag="PLM04_04765"
     CDS             complement(7055..7840)
                     /locus_tag="PLM04_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HON02421.1"
                     /translation="MRNFSFLFFTLLIVLFTGCTSQKKIAYFYGVNEKSVANINQSFT
                     KGHEARICTGDMLSITVSGLDPEAVAPFNLPLVSYANPGSTQIYASPVLQSYLVDING
                     YINFPIIGPIKLVGLTKSEAIAYISELLSPYLKNAIITIQFLNYKVTVLGEVLRPGQY
                     SINNERVTVLDALGLAGDMTVYGKRENVLLIRENFGKLEFVRLNLNSEDVFTSPYYYL
                     QQNDILYVEPNVVRSISAQNIPLYLSTISTFATLVTVIYSVTK"
     gene            complement(7980..9827)
                     /locus_tag="PLM04_04770"
     CDS             complement(7980..9827)
                     /locus_tag="PLM04_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HON02422.1"
                     /translation="MDSINKTDFSELITDKASQNVSAELSVGENNSAVEDQNTESTPS
                     VVSNYSTLSNEELIEALKKCLNQEVEEVKNDVESIKQAFYRNHRAEVENQKKLFLENG
                     GVEEDFVPKPNELEETFKSLLKEFRDKKAEHLARVEKEKQDNLLKKQQILEKMKILAD
                     AKDDVSTHINEFRALQQEWKSIGQVPPSSLSELFKQYNAYQEAFWDLIKINNELREYD
                     FKKNLEAKIQICEAAEKLMDEPDVVTAFQHLQKLHNEWHEIGPVSRELREEIWQRFKN
                     ASTAINKKHQTYFESIRKIEEDNLAAKTALCEKIESIDFSVLKTFSEWEEATKNVLAL
                     QDEWRTIGFAPRKLNQKVFERYRHACDAFFSAKAEFFKQVRNSYAENYRKKVELCENA
                     ESLKDNTDWKETSEKFIQLQKEWKAIGPVPRKYSDELWKRFISACDYFFDQKKKNTNS
                     LHGEELENLEKKKNLIARINSLKKSDHPNETVATLKALIEEWSTIGHVPFKEKDKVYK
                     DFRAAVDKKYEELNIDWVSRRLETFKNNLKDMNTKGQRKLIHERDKLLHARERLNSEI
                     ATYENNLGYFTSTTQKGSKVILELERKIDTLKEESRLIEEKIRLIEDNL"
     gene            complement(9999..10775)
                     /locus_tag="PLM04_04775"
     CDS             complement(9999..10775)
                     /locus_tag="PLM04_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HON02423.1"
                     /translation="MTTSQNNKIAFVSGATSGFGRAIAFRLGKLGYRLIITGRRNERL
                     QRISQQLKEEYSTDVFSLCFDIRDKNACEQAIQSLPADFQNIDLLVNNAGLAAGASSF
                     EESDIADFEQMVDTNIKGLLYITKLIVPKMVERKSGHIINISSIAGIEVYPGGSVYCA
                     TKHAVNAITKGLRIDLVKYGIKVSSISPGMAETEFSLVRYHGDAEKAKAVYAGLVPLN
                     AEDIADAVEFIVTRPKHVSINDIQINPAQQANTYISFRNT"
     gene            complement(10805..12049)
                     /locus_tag="PLM04_04780"
     CDS             complement(10805..12049)
                     /locus_tag="PLM04_04780"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HON02424.1"
                     /translation="MMEIKLNTIEEAINDIRNGKFVIVVDDEDRENEGDFICAAEKVT
                     PEMVNFMITYGRGVVCAPLTEERCEELELEMQVENNTSMHSTPFTVSVDKLEGCTTGV
                     SAADRAATLRALADPSSTPETFGRPGHIFPLRAKSKGVLRRAGHTEAAVDLARLAGLY
                     PAGALVEIMNEDGTMARLPQLMEIAERFDLKIITIKDLIAYRLKFESLIEKGEMVNLP
                     TAYGNFKLIPFRQISNDKEHVALIKGEWEKDEPILVRVHSSCVTGDIFGSLRCECGEQ
                     LHKAMEMIEKEGKGVIVYMNQEGRGIGLMNKIKAYKLQEQGMDTVEANLHLGFDADER
                     DYGVGAQILKDLGVTKIRLMTNNPVKRIGLEAYGLSIVENVPIEIKPNPYNAFYMQTK
                     KERMGHTLRYVNGDKKIDTDIL"
     gene            complement(12060..13547)
                     /locus_tag="PLM04_04785"
     CDS             complement(12060..13547)
                     /locus_tag="PLM04_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HON02425.1"
                     /translation="MFKIKKIDIYVFKNFISLFIVTFLICIFILLMQFLWRHFEDLVG
                     KGIGWSVLFEFFFYSALSLVPMALPLAILLASLMTFGNLGETLELTAMKAAGVSLFQI
                     MRPLLFFISLICVAAFFFSNFALPMAQTKLWTLIFSLKQKSPELEIPVGEFYTGINGY
                     NIYVREKDGKILKDLMIYDFSAGFDNAIVMVADSGEIQLSSDKKNLLLSLYSGESFEN
                     LKNQQIGSSPSSIPYRRESFRTKEIVVDFNAEFNRFDESLLNDQHISKNISQLSQSID
                     SVNKIVRSMEIQQSKEIMQHPYINWSINQPESTLLPEEKLSTYSFDSLFLSLNEESMF
                     RAIDLAISEVQQIKSSIDFNKIMLEQQIMYVRRHWIEWHRKFTLSFACLIFFFIGAPL
                     GAIIRKGGFGMPVVISVVMFIIYYIIDNTGYKMAREGIWSTFQGMWLSSAVLLPIGIF
                     LTYKAVTDSALFNSENYTRIWNSLVQKIKIWIDKFFALQLKKRHA"
     gene            complement(13714..14097)
                     /locus_tag="PLM04_04790"
     CDS             complement(13714..14097)
                     /locus_tag="PLM04_04790"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HON02426.1"
                     /translation="MQKSIFTLEYPLKSASANFLWTKIGSPLGLSEWFANEVDLEDSK
                     YVFKWEEFQQSARLLELKPNKSIRFQWLDDLDTPAYFEFRIDTQELTGEVTLVITDFA
                     WEDEKEDAILLWNYQVDELKRIAGM"
     gene            complement(14233..14715)
                     /gene="cdd"
                     /locus_tag="PLM04_04795"
     CDS             complement(14233..14715)
                     /gene="cdd"
                     /locus_tag="PLM04_04795"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HON02427.1"
                     /translation="MFKKSTKVTYSVCRFDELTSLQQQLIDKAKEQVQNAYAPYSGFY
                     VGAALELENGEVIAASNQENSAYPSGLCAERVAVFYANAQYPDIPVKTLAIAAFTNGE
                     FLKMPVTPCGACRQVLLETELRFEKDITILLYGSQEIYIISCVKDLLPLSFDKLQLQK
                     "
     gene            14989..15933
                     /locus_tag="PLM04_04800"
     CDS             14989..15933
                     /locus_tag="PLM04_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HON02428.1"
                     /translation="MYSVASKFRLFIVLLFATNIAFGQQLNKNYLSYIEKYHQLAIKQ
                     QKKHGIPASIILAQGLLESSAGQSQLSQLSNNHFGIKCNSDWNGETISWDDDEKNECF
                     RKYKNVADSYEDHSLFLKNRPRYAFLFQLDKTDYQGWAHGLKKAGYATDPTYAYKLIS
                     LIENYNLNRFDKQIFDNNYAERMEIQINTSIPQTIQKVSDYVTHKKFKNNGVPYVIAV
                     PCDTYANIAQEFNLSEETLRSYNEVSATTALQPGEKVYIRPKKNKASKNYPVHVVQAG
                     ESMHDIAQFYGIKTEKLYKMNRIPFTEGAQVGQVLKLR"
     gene            15956..16246
                     /locus_tag="PLM04_04805"
     CDS             15956..16246
                     /locus_tag="PLM04_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HON02429.1"
                     /translation="MRRKNTELLSEIIRQMLKDQHLEKPLYEKRIIDAWTSVLGPQIK
                     KYTGHLEVKNGILYVSINSAVLRHDLFLSREEIKNALNKEVGHEVINAIVFR"
     gene            16412..17956
                     /locus_tag="PLM04_04810"
     CDS             16412..17956
                     /locus_tag="PLM04_04810"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HON02430.1"
                     /translation="MSAKKNIIALRQKKAIIEKGGGEKAIEKQLAMGKMTARDRIRAL
                     LDEGSFHEYDLFVQHEEKNFGMDTKDLPGDGVITGTGSINGNPVCIYAQDFTVMGGSL
                     GIQHARKITKIMDHALKMKMPCIGINDSGGARIQEGIGALAGYGEIFYRNTMASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVENISKMFITGPNVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYALSEKECLQQIKELISYIPHNNEEKAARVEPKKPKFKKKIENIIPTN
                     PKLPYDVREIIYALSDDSKFFEVHQLWAPNIVVGFARIEGETVGFVANQPMYLAGVLD
                     CDASDKAARFIRFCDAFNIPLITLEDIPGYLPGVDQEHAGVIRHGAKVLYAYSEATVP
                     KITVILRKAYGGGYIAMNSRHLGADFTFAWPEAEIAVMGPEGAANIIFKKEIMEAEDP
                     DAMRQLKVKEYIEKFANPFVAAGKGYIDAVIEPSETRELLIHSLSVSRKKFDYRPTRK
                     HGNIPL"
     gene            18157..18504
                     /locus_tag="PLM04_04815"
     CDS             18157..18504
                     /locus_tag="PLM04_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HON02431.1"
                     /translation="MKKNKLSIQENQELIDFAVTARKYKTQLTKKFLNRKKWENPSPF
                     EIYSFIPGTVVDIFVNEGDIVEEETPLMTLEAMKMQNKIEMPFTAKIKKINVKKGEQI
                     PKDFLIFELEPVE"
     gene            18808..19374
                     /locus_tag="PLM04_04820"
     CDS             18808..19374
                     /locus_tag="PLM04_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HON02432.1"
                     /translation="MNQTDKQFDQIAGICRNIFIQKLKDYGASWRLLRPTSVTDQLYI
                     KANRIRNIQVKGFSKVDEGIQSDLIGIVNYAIIGLIQLEKGFAEKPDLSVEETIALYD
                     KYMQQAKELMMDKNHDYDEAWRHMRIESMVDLILMKIERTKQIEENDGKTLISEGIDA
                     NYFDMINYAIFALIKMDENDCQQKNEKI"
     gene            19402..20565
                     /locus_tag="PLM04_04825"
     CDS             19402..20565
                     /locus_tag="PLM04_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HON02433.1"
                     /translation="MSSDIYKNKKMAFPAEQKGKKILFVRICSILLGALFVFSGFVKA
                     IDPLGTTYKIEDYLNAFGGPFPQLVFLAFPAAVFLSTLELVMGLNLLLRIYVRFTTWV
                     AAVFLFFMTLLTFYIALKNPVTDCGCFGEAIVLTNWQTFIKNIFFIALSTFLLINRKH
                     LYPLVLPSLEAIFTVSFVIVGVGISVYSYFHLPIMDFRPYKVGVNIPEAMKIPEGAKP
                     DVYETTFIYEKEGKQKEFNLENYPKNDKSWKFVDQKTKLISKGYRPPIHDFTILNKDN
                     EDISDIILSQPDSTFLLIMYDINKTSQEGAKKAERIFQKTQQNGHSFYAITASTDNDI
                     QKFTERTKISFPFYKADPIMLKTVIRANPGLVLIKDGTILGKWNWRDFPENFY"
     gene            20647..21402
                     /gene="tpiA"
                     /locus_tag="PLM04_04830"
     CDS             20647..21402
                     /gene="tpiA"
                     /locus_tag="PLM04_04830"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HON02434.1"
                     /translation="MRKNIVAGNWKMNKTLPEGLALATELDKMLSNYQPKCEVIIAPP
                     FIHLASITATIDTKKIGVSAQNCADKESGAYTGEVSAAMVKSTGANYVILGHSERRTY
                     YGETPVLLKEKVNLALKNELTPIFCIGEVLAERESGIYFDVVKSQIEGSLFHLSAEDF
                     SKIILAYEPVWAIGTGKTATPQQAQEMHAFIRQIITEKYGKNIAENTSILYGGSCNPG
                     NAKELFANEDVDGGLIGGASLKAADFKAIIDAF"
BASE COUNT         7272 a         4141 c         3680 g         6640 t
ORIGIN      
        1 aggtaaaaca ttccattcct ttgtcctgat aaaataaatg ctttacttcg ggctcttccc
       61 attaaaaaac tacaaagatg aaacatttaa ctgtagagga aaggtacgaa atttgaacag
      121 tattacaaca aggattgaaa caaaaagata ctctattgac taccggaaaa gaaaagaaaa
      181 aaaatatcgt tgccttccga tgatgcagtt gtaaaatctc tatctttttt ccctcaggaa
      241 agccactaaa aaatggacaa tccccattca aaaccaggac atttctttgt cgaaagagta
      301 aaattttaat ttttaaacca aatttttaat ttacacgctt cgaggaaaag tattacaaat
      361 tcgcagcgtt atcaactact ccaacactga ttatttgctc gaaaagttaa acggatatag
      421 aaaaaggatt tgattcatat tgcaggattg caagcaacaa cctttggtgt tgagttaagg
      481 aaaaaatagc ataaaacata aaaaatctgc tttcaaaaaa tcaaaacata tcaaaagcag
      541 atttaaaatt taaccaaatg gtcttatccg taaaatttct tatccaaatt catccaaagc
      601 agataaaatt cttatttgct tgaacaatta ttgcatacac cataataaaa tatttcaact
      661 gaattgactt tgaaattatc attcagtggc aattgaaaaa cttccggacg aacatcaaaa
      721 acatcagaaa ccttcccaca ttgctggcaa ataaaatgag catgcggggt agtatcgaca
      781 tcataacgcg cattcttctc gtcgatggtc aacattcgcg cagctcccct ttcaatgaaa
      841 attttgagcg tattgtaaac tgtagttttc gaaagtgtcg gcatagaagg gcttaaaacc
      901 gaaaaaattt catcaactgt aggatgcgtt ctgttattca acagataatc cataactgct
      961 gtccgttgaa ccgatggttt tatagaatgt tgaataagat attgttgtga ataggctata
     1021 gaataataca taatggtttc ttatttttgc tgggcaaaga tacaatatac tattgacact
     1081 gacaagattt caaacttgaa ttaaatttaa atttttactt cccattcggc ctactgttta
     1141 aaaaatttaa aaggttatga gcttgtcgca tcataacctt aaaaagcaaa aaaaagaaaa
     1201 aaaattatat tacacccaaa tccttgagca tagcattggt tgcgcgcaaa ctttcagaac
     1261 tttttatgaa taattccttt tcttcagaat ttaggtgata atctaaaatt tcttcaaccc
     1321 catttctacc tatcagcaca ggaacgccga tacaaatatc gttttgtcca tactctcctt
     1381 ccaagtaaac agagcagggt ataattttct tttcatcatg aataatagct tctaccagaa
     1441 acgcagcagc tgctccaggc gcataccaag ccgaagtacc caacaaactt gtaatagtag
     1501 ccccacccag catagtatcc gaaactactt tgtccaatgt ttctttatcg agcaaatcgg
     1561 taaccggacg acatttataa gctgcataac gtaccagtgg aatcattgag gtgtcgccat
     1621 gagctccaat gacaaaacct tcaatctcat tttgattagc attcagagct ttacctaaat
     1681 aacatttaaa acgagctgtg tcgagtgttc ctcccattcc gattactcta ttgcgaggga
     1741 gacctgcgat tttataagta aggtaagtca tggtgtccat cggattactt actattacca
     1801 aaatagcatt gggagaatat tttaaaacat tctcaactac acttttaata atgccggcat
     1861 taacatctac caattcttca cgtgtcatac ccggtttgcg aggaactccc gaagtgatga
     1921 ctactacatc cgaattggcg gttttttcat aatcgttcgt acaaccggat atcctggtgt
     1981 taaaatgaag caaagtagct gtttgattta tatctaaagc tttaccctca gctattcctt
     2041 gtttaacatc aagcattaca acctcatcgg ctatttgata gaaagaaaga acatttacac
     2101 atgttgcgcc aacattcccg gcgcctatca cacttacttt tgacatacta ttttaattta
     2161 aattattaat ccgagaaaag cattgcaaaa ttacaaatta aaatgattag tgaaaaattt
     2221 ctttaattat tttagcataa atatatttat ctcgagaaac gacattgtta tggcattcca
     2281 aacagaaaaa ttaaacaaat ctcatttgaa aataaacaaa ataaacgtaa ttttgtagaa
     2341 tgtaaattat atgtgattat gaaacccaca tgtgttgcat acacaaacaa gaatgaatat
     2401 attattcaga catgttggtt ccatcagacc tttaaaaata aattatattc tgatgaaaaa
     2461 ttatttttat tcacttgtca ttattatttt gctgattggt tgtcaaaata aaaattcttt
     2521 tactgttgaa gggaacatat cttcagccaa aggagaaaaa ttatacatag aacacatggg
     2581 gttattaaaa acaacttctg tagattcagt taaacttggt aaaaacggaa aatttcattt
     2641 taccataaaa cgacctgaat atcccgattt ttaccgcttg cgactgaacg ataaattaat
     2701 catttttgcc gtagactcaa cagaaaaaat tacaatcaaa gcaagtaaag aagatttgcc
     2761 atacaattac acactaacgg gttctgaaca atcgcttaaa attcaacaat tgaggaaatc
     2821 ggtagccaat attcaaaaca agatcaacaa attttcaagc aacttagctc cagaagagca
     2881 aaatcagcta atgaaagaaa ttgaaaaaga cattgaaaat cataagcggg aagctcaaaa
     2941 attaatcttg gaaaatccac gctctacagc tgcttattat gcactttatc agaaagtgaa
     3001 tgatttttat ttgttttcac cttacgtaaa ggaggataga tcttattgcg cggctgttgc
     3061 cactgcttat cacactttca tgcctaacta caccagaagt caaaaccttt acgcaacggt
     3121 aatggacgct ataaaaaatg aaagaaaagc agaaagtcaa ctacgattgg aagagttatt
     3181 tagaacaaac agcaccggct acattaatat tgttttgccc gataagaatg gaatacagca
     3241 aaaactgtcc gatttggaag ggaaaattgt tttgatcgat ttttcaattt atacagctcg
     3301 tgaaaacgtt gaacatactt tttcgctgcg tgagttatac aacaagtatc acgaacgcgg
     3361 ttttgaaatt tttcaagttt cacttgatga aaatcgcttt ctttgggaaa aatccgttca
     3421 gaatattcct tggatttgtg ttcgtgacga acgaggaccc aatacgccat atgtactttc
     3481 ttacaacatt tctactatac cggcatcttt tttgatggac aggcaaggca atattattgc
     3541 ccgtgatttg gatttcaaac aattggacaa acaaatagca aaacttttgt agtttatttg
     3601 atacaataaa aagtttaatc gtaatctata ccgctcaatt cggttgtctt tctcaacaaa
     3661 aaatcgaatg agaaatttca ttatcaatac tgcactctct atcggattcg atgcttgcgg
     3721 aataaccaaa gccgaaacac tttctgcaga tgcaagttat ttgcagtcgt ggctaaaagc
     3781 aggctgtcac gcaagcatga aatatctgga aagaaatttc gacaaaagag tgaatccaaa
     3841 attactggtt ccggggtgtg aatcaataat tgtagttctg atgaactatt ttccatccga
     3901 aatgcaattg tcagccgcgc cccaaattgc aaaatacgct tacagcaaaa tcgattacca
     3961 caaagtttta aagacaaaac ttcaacagtt ggaaaaagcc atcacagaaa agtttggtaa
     4021 aaacagtttt aacgatgaag tacaacatat ttttgtggac tctgctcccg tactggaacg
     4081 tcgatgggca gaaaaagccg gtttgggatg gattggaaaa aacaaacaac tcatccatcc
     4141 gggattgggt tcttatgtct ttatcggaat tttaatgatg aatgccgaaa cagaatatga
     4201 tttgccaatt aaaaaccgct gcggaaactg ccgaaaatgc atcgactcct gtcccactca
     4261 agcgctatcg gaagaatggc tggatgcaaa taaatgtatt tcttatctga ctatagaatg
     4321 caagacaaat attcctgaag aatttcaggg caaattatcc aattacgcgg tcggctgcga
     4381 tatttgtgcc gacgtttgtc cgtggaacag gaaatgggca caacccaaca ggcatcccga
     4441 attggaaaca gcttctccca taaaaaaatg gaactgcaag caatgggaaa atctcaacca
     4501 agaggaattt gaatcagcgt tttcgaaatc tgccatcgaa tacgttggct atagcaagct
     4561 gaaagaaaac atttccatat tgatggaaaa gttcaaaaag gactaaaaaa ttaataaagt
     4621 gtcagtttat cacttattct tttttagctt gttgagtctt ttttccaaag cattttgagt
     4681 agtctctcta tatctgtact tatatttctt ttcattttta tatccatatc cataatgaga
     4741 aacatcattt gttccattca atacagtagc catgttttta agctttttgg attcatacgt
     4801 atgattaata aaatgtaaag cttcttttgg cgtatattta agacgagcta cacaaatagt
     4861 attgtccacc aaacgattga gttggaaagt atcggaaaca attccaatag gagccgaatc
     4921 gagaataata taatcgtaac gggatttcaa ttccctgatc aattcatcaa gtcgttggct
     4981 catcaatagt tcagcgggat tgggaggaac tgctcctgct aaaataacat caagataggg
     5041 atgtacctcg gaaggctgaa taatttcatc cattcgacaa ttcggatcag ccaaaaatat
     5101 agtcaacccc ttattgttct tttttaaatt aaaatattca gccaacatgg gatttctgat
     5161 atctacgccc accagaatga ctttcttttt cattaaggca aaggacatgg caagatttgc
     5221 tgcaataaag gtcttccctt ctccgctgat ggttgaggtt accaatatga caggtgattt
     5281 tatgtcggaa aacataaact gcaaattgga acgcaccagc ctgaacattt caactatggg
     5341 agttgttcga ccttctttca ctacaatatt ttccgtttct ttacttaacc caatagttcc
     5401 aagaaatggt gccttgacaa atttctgaaa ttcttttcta tcttgaactg tattattaaa
     5461 aaattccatc agatacatca ccaatatcgg caatccgagt cccaaaatca acgcaagtaa
     5521 aaaaataatc attcttttgg gagaaatagg tgaaccggtg gtataggctg catcaattgt
     5581 tctggctgat ggaatagtac ttgccagatt tagagcattt tcttctcgtt tctgaagcaa
     5641 aaaaacatag agcgattcct tgatctgctg ctgacgttta atttccataa actgacgctc
     5701 ttgagtggga acgctcttaa ttcttgaagc aaattcattg ttgtaggcca ttaaatcttt
     5761 cttagaaata accaatccat cttttacacc ggcaatactt tttacaatgt tactgcgaat
     5821 ggcattgatt tgttgctcca tttgggtaat aacggggttt tgttcgttcg agcttctcaa
     5881 aattttcatt cgttccaata aggcaacatt gtattcttca ataagttttt gcaaggctgg
     5941 atctttaata cccaagttcg caggaatcag attgaactgg tttttactct cgttgatata
     6001 actttcaaca taatttacca aattgagctg tgtttctata tcggcaagtt ttttttcata
     6061 ttcactagcc gtttcgagat aaagttgggc ttcagatgaa atatcagtta atccgtgagt
     6121 ctttttatag ttttctacat ttaattctac atcgagcaat tcgctgctaa taagccttaa
     6181 acgactctca ataaaatcgg cagtattggt caatatcata tttttatcaa aaatggcatc
     6241 caaattataa agctcaacca atttatcgag catgactttt gccttgtgag gtgtaggcga
     6301 aacagtacta atgctgatgg cattagaatt cttgttcact gtagccacag aaatggacgc
     6361 tgcgagctga ttggttaagg cagcaagtgg ataggaaata atttttatct tttgtccact
     6421 tttcaaggga ccatttacaa aaaatcgaat tgaaccaata ggtgtagaaa gtggtgtgga
     6481 aagatcggat actcgatatt ttttttcaat aatttctcca tattgaaatg aaatatcgta
     6541 accttttgaa gtaggagaca atattaagat tatcttttgc tttaaagtgt cattgaaatt
     6601 atttgacacg aaaatctgaa ccggactgat gggataaact tcatcatatc ttaatccatt
     6661 ttttacatag tattctgttc ctagatttaa ggagtcgatt acttgcctaa caagagtttt
     6721 agaagaaagt acctgaattt catcttccac ctctttcgaa actcccaaca accccatatt
     6781 agaaaacatg gtcatgttgg acaattcagg agtcgaattt tgattacgaa gcaagatggt
     6841 ggtctgaacc tgatatttgt tgtgggtaat tttcagatac aggaaggcta cgataaaaca
     6901 aaaaacaatt gaaataacaa accaatgcca tttactcaga tattttatta gtaattgcct
     6961 taaatcaatc acttcatttt cgtttgcagc aacgcgagaa tcaatattgt tttccattta
     7021 taacagataa aaataacaca atttaaacta aaaattattt tgtaacacta taaataaccg
     7081 taaccaaagt agcaaacgta gagatagtag atagatacaa cggaatattt tgagccgaaa
     7141 tgcttcttac aacgttgggt tctacatata aaatatcgtt ttgttgtaaa taataataag
     7201 gagaggtaaa cacatcctcg gaattcaaat ttaaacgaac aaattccaat tttccaaagt
     7261 tttcacgtat caacagcaca ttttctctct ttccgtaaac tgtcatgtcg ccagccaaac
     7321 ccaaagcatc caacactgta actctttcgt tgttgatgga atattgtccg ggacgaagta
     7381 cttcacccag aaccgtaact ttataattca aaaattgtat ggtgataatg gcatttttca
     7441 aataaggact caataactca ctaatataag caattgcttc cgatttggtc aatccaacca
     7501 attttattgg tccaataatc ggaaaattga tatagccgtt aatatctacc aaataagatt
     7561 gcaaaacagg agaagcgtaa atttgtgtag atccaggatt ggcataagaa accaaaggca
     7621 agttgaaagg agcaacagct tcagggtcta agcccgaaac tgttattgac aacatatcac
     7681 ccgtacaaat acgcgcttca tgccctttgg tgaaagattg attaatattt gcgaccgatt
     7741 tttcattaac accataaaaa taagcaattt ttttctgaga ggtacatcct gtgaataaaa
     7801 caattaacag agtaaaaaaa aggaaagaaa aatttctcat aatcagttat ttttcagtat
     7861 tcatactcaa aataaaaaat ttacaaccgt taggtttata acaccattta tcaaaaaaat
     7921 taacgacttt aaaatgaaat tattttatca ttttaaagtt aaaaaaattt ccttgcgccc
     7981 tataaattgt cttcaatcag cctgattttt tcttctatta agcggctttc ttctttcagg
     8041 gtatcaattt ttcgttccaa ttcgagaatc actttactgc ctttttgggt agtagaagta
     8101 aaataaccca aattgttttc ataggttgct atttcagaat taagtctttc ccgtgcatga
     8161 agcaatttat ccctttcgtg aatgagtttt ctctgccctt tggtattcat gtctttcaaa
     8221 ttatttttga aagtttccaa gcgacgactt acccaatcga tattgagctc ttcatatttt
     8281 ttatctacag ctgcacgaaa atccttgtaa accttatcct tttccttgaa aggtacatga
     8341 ccaatggttg accattcttc tataagagct tttaaagtgg caaccgtttc attgggatga
     8401 tccgattttt ttaatgaatt gattctggct atcagatttt tctttttctc gagattctcc
     8461 aattcttctc catgtagaga attcgtgttt ttcttttttt gatcgaaaaa ataatcgcaa
     8521 gcagatataa aacgtttcca aagttcatcg gaatattttc ttggcaccgg accaatggcc
     8581 ttccattcct tttgtaattg gataaatttt tcggaagttt ctttccagtc ggtattatct
     8641 tttaaagact cggcattttc gcaaagctca acttttttgc gataattttc ggcataactg
     8701 tttctcacct gtttgaaaaa ttctgcttta gccgaaaaaa aggcatcgca agcatgccgg
     8761 taacgttcaa aaactttttg attgagtttt cgcggtgcaa aaccaatggt tctccattca
     8821 tcctgcaagg ccagtacatt tttagtggct tcttcccatt cactgaaagt ttttaataca
     8881 gaaaaatcaa tggattctat tttttcgcaa agagcagttt tggcagccaa attatcttct
     8941 tctatctttc gaatggattc aaagtaagtc tgatgttttt tattgatagc agtggaagca
     9001 tttttaaaac gttgccagat ttcttctcgt aactcacgtg aaacaggacc gatttcatgc
     9061 cattcattat gaagcttttg taaatgttga aatgctgtaa ccacatcggg ctcgtccatc
     9121 aatttttcgg ccgcttcaca aatctgtatt ttagcttcca gatttttctt gaaatcatat
     9181 tctctcagct cgttgttgat tttaattaaa tcccaaaaag cttcctgata agcattgtat
     9241 tgtttaaata attccgacaa actggaggga ggaacctgtc caatagattt ccattcctgc
     9301 tgcaatgctc tgaattcatt aatatgggtc gacacatcgt ctttggcatc ggcaaggatt
     9361 ttcatttttt ccagaatctg ctgctttttc aacaggttgt cctgtttttc tttctcaacc
     9421 cttgccaaat gttcagcttt cttatcgcga aattccttta acaaagattt aaaagtttct
     9481 tccaactcat ttggttttgg aacaaaatct tcttctaccc ctccattttc gagaaaaagt
     9541 tttttctgat tttcaacttc cgcacgatga tttcgataaa atgcctgttt aatactttcc
     9601 acatcatttt taacctcttc aacttcctgg tttagacact ttttcaaagc ctcaatcaat
     9661 tcttcatttg aaagagttga ataattcgaa acaacagaag gtgtcgattc ggtattttga
     9721 tcttctacag ctgaattatt ttcgccaaca gacaactcag cagaaacatt ctgcgatgct
     9781 ttgtcggtaa tcaattcaga aaagtcggtt ttgttgattg aatccattta aagtaacgtt
     9841 attcatgatc tgttctccca ttttttagcg agaacgatgc tatttctttt caaaaacatt
     9901 tttatattca caaaagtata ataattattt taaatatcga tgatgtacat taaaagattt
     9961 taagctttct ttgtcgaatc ggtttattat tgataatttt acgtatttct aaatgatata
    10021 taggtatttg cctgctgtgc aggatttatt tgaatgtcat taatggaaac atgctttgga
    10081 cgggttacaa tgaattccac cgcatcggct atgtcctcgg cattcaatgg aacaagacca
    10141 gcatataccg cttttgcttt ttcggcatct ccatgatagc gcaccagcga aaattcggtt
    10201 tcggccatac cgggagaaat ggaacttact tttattccat attttaccaa atcaattcgc
    10261 aaacctttag taatggcatt cacggcatgt ttggttgcac aatatacact tccgccggga
    10321 tatacttcta ttcccgcaat tgaagaaata ttaataatat ggccactttt ccgctccacc
    10381 attttcggca ctattagttt ggtaatatac aacaaacctt taatgttggt atctaccatt
    10441 tgttcaaagt cagctatatc gctctcttca aaagaagaag cccctgctgc caaaccggca
    10501 ttgttaacca acaaatcgat gttttgaaaa tctgcaggca acgattgaat agcttgttca
    10561 caagcatttt tgtcgcgaat atcgaaacag agagaaaaaa catcggtaga atattcttct
    10621 tttaattgtt gggaaatccg ctgcaatctt tcgtttcgtc gtccggtaat tatcaatcga
    10681 taacccaatt ttcccaatcg aaaagctatt gcacgtccaa aacccgacgt ggcacctgag
    10741 acaaaggcta ttttattgtt ttgagaagtt gtcataaaac aatttagtaa aaatagaagt
    10801 atttttaaag aatatcggta tcgatttttt tatctccatt tacataacgc agcgtatgtc
    10861 ccattctttc cttttttgtt tgcatataaa aagcattgta aggatttggt ttaatttcta
    10921 tcggcacatt ttcgacaata gacaatccgt aggcttcgag tccaatgcgt ttaacaggat
    10981 tgttggtcat caatctgatt ttggtaacac ccaaatcctt caagatttgc gcaccaacgc
    11041 cgtaatcgcg ttcatcggca tcaaagccga gatgcaaatt ggcttcaacc gtatccatgc
    11101 cctgctcctg caacttgtaa gcctttattt tattcatcaa tccaatacct cgtccttcct
    11161 gattcatgta aacgatcaca cccttgccct ccttttcaat catttccata gctttgtgca
    11221 actgctcgcc acattcacat cgaagcgaac caaaaatatc tcccgttaca caagaggaat
    11281 gaacacgcac caaaatgggc tcatcttttt cccattctcc cttgattaag gcaacatgtt
    11341 ccttatcatt ggaaatctga cgaaaaggaa tcagtttaaa attaccgtat gcagtaggca
    11401 aattgaccat ttcgcctttt tctatcaaag attcaaactt caaccgataa gctatcaaat
    11461 ccttaatggt tataattttt aaatcgaaac gttctgcaat ttccatcaat tgcggtaaac
    11521 gagccatagt tccatcttcg ttcataattt ctaccaaagc tccagccgga taaagaccgg
    11581 ctaaacgtgc aagatcaaca gcagcttccg tgtgaccagc cctgcgcaat acgccttttg
    11641 attttgctcg aagtggaaaa atatgaccgg gacggccaaa agtttcggga gttgatgaag
    11701 gatcggcaag tgcacgtaaa gtagctgctc tatcagccgc cgaaacgcct gtagtgcatc
    11761 cttccaattt gtctacgctt actgtaaaag gtgttgaatg catggaagtg ttgttttcta
    11821 cctgcatttc gagttccaat tcctcacatc tttcttcggt caggggagca caaactactc
    11881 cacgaccata agtaatcatg aaattgacca tttcgggcgt aaccttttcg gcagcgcaaa
    11941 taaaatctcc ttcgttttcg cgatcttcat catccaccac aataacaaat ttgccattac
    12001 gaatgtcatt aatggcttct tctatagtgt tcaattttat ttccataatt tataaaaatt
    12061 taagcatgtc ttttttttaa ttgcagtgca aaaaacttat ctatccatat cttgattttt
    12121 tgcaccaaag aattccaaat tcttgtgtaa ttttctgaat taaaaagagc agaatctgtt
    12181 actgccttat atgtcagaaa aatgccaatg ggcagcaata cagccgaact cagccacatc
    12241 ccttgaaaag ttgaccaaat tccttctctg gccattttgt atccggtatt gtcgataatg
    12301 taataaataa taaacatgac aaccgaaata actaccggca tgccaaatcc tcctttacga
    12361 ataatggctc ccaacggcgc tccaataaaa aagaaaatca gacaggcaaa ggaaagagta
    12421 aatttccgat gccattcaat ccaatgtcgg cgtacataca taatttgctg ttcgagcata
    12481 attttattaa aatcaatact gcttttgatt tgctgaactt cagaaattgc caaatcaatg
    12541 gcacgaaaca ttgattcctc gtttagcgac aaaaaaagag aatcaaaaga ataagttgaa
    12601 agcttttcct cgggcagcaa agtagattca ggctgattga tgctccaatt gatataggga
    12661 tgctgcataa tttcttttga ttgttgaatt tccattgacc gcacaatctt attgacagaa
    12721 tcaatagatt gagaaagctg tgaaatattt ttgctaatat gctggtcatt caacaaagat
    12781 tcatcaaaac gattgaattc ggcattaaaa tcaaccacta tttcttttgt tcgaaaagat
    12841 tcacgtcggt aagggatgct ggaaggagaa ctgccaattt gctgattttt aagattttca
    12901 aaactttctc cactgtaaag ggaaagcaat aagttttttt tgtctgatga cagctgtatc
    12961 tcacccgaat ctgccaccat tactatggca ttatcgaaac ctgcagaaaa atcataaatc
    13021 atcaaatctt tcaatatttt cccatctttt tctcgaacat aaatattata accattgata
    13081 cctgtataaa attcgccaac aggaatttcc aactcaggag atttttgttt cagtgaaaaa
    13141 atcaatgtcc aaagctttgt ttgagccatt ggtaaagcaa aattggagaa aaagaaagcg
    13201 gccacacaaa ttaacgagat aaaaaataac aacggacgca taatttgaaa aagagatacg
    13261 cctgctgctt tcatggctgt aagttctaaa gtttctccca gattaccaaa agtcataagg
    13321 gaagctaaca aaatagcaag tggtaaggcc ataggaacaa gagaaagagc ggagtagaaa
    13381 aaaaattcaa ataataccga ccaccctatt ccttttccga ccaaatcttc aaaatgacgc
    13441 cacaaaaact gcattaaaag aataaaaatg cagatgagaa aagtaacgat gaaaagcgaa
    13501 ataaagttct taaatacgta tatatcaatc ttttttatct taaacatatt cgttttctta
    13561 aaattacaaa attacgctaa aaagtttgat tttacattat tcgtagcatg aaatatgctt
    13621 aaaaaatgaa acttatcagc aaaaaaaagc ataaaaaaac cacttgtcgg acaaacaaat
    13681 ggctaattct tgagaaacaa gcaacagcag cttttacata ccggcaatac gtttcaattc
    13741 gtcaacttga tagttccaca acaggatagc gtcttccttt tcgtcttccc atgcgaaatc
    13801 ggtaataacc aatgtaactt caccggtaag ctcttgcgta tcgattctaa attcaaaata
    13861 agcaggagta tccaaatcgt cgagccattg aaaacggata cttttattgg gtttcagttc
    13921 caaaagtcgc gcactttgct gaaattcttc ccatttaaaa acgtattttg aatcttccaa
    13981 atcaacttca ttggcaaacc attccgataa gccaagcggc gaaccaattt tagtccacaa
    14041 aaagtttgcg gaagccgact tgagaggata ttccaatgta aaaatcgatt tttgcatttt
    14101 tctcttattt tttcgttctt attctaactc tcaaattcct aaaaaacatt attacattaa
    14161 cgactgcaat ataatgaatt aaaacgagca ttcaaaattt ttcactcact aattcagcaa
    14221 aaattgtggt actcattttt gtagctgcaa cttgtcgaaa ctcaaaggca acaaatcttt
    14281 cacgcaagag ataatataaa tttcctgact tccataaaga agaatggtaa tatctttttc
    14341 aaaacgcaat tctgtttcga gcaaaacctg ccgacatgct ccgcagggcg taacgggcat
    14401 ttttaaaaat tctccgtttg taaaagccgc aatagcgagc gttttcacag gtatgtcggg
    14461 atattgggca ttggcataaa atacagctac tctttcggca cataaacccg aaggataagc
    14521 cgaattttcc tgattgctgg ccgcaataac ttcaccgttt tcgagctcaa gagctgcacc
    14581 aacataaaat ccggaataag gtgcataagc attttgtacc tgttctttgg ccttatctat
    14641 caattgttgc tgcaatgaag tcaattcatc aaaacgacag accgaataag taacttttgt
    14701 tgatttttta aacatagtag aatctgattt aatgatactt tcaaaaaatt ttcctttagc
    14761 agggggtaca aaagtaaaaa aataatcaac aaaacgctca tttttgagaa aataaattat
    14821 atttttgcaa taaactaaac tttattagcc ggtttaatct atcaagctga aaactacggg
    14881 aatttttcta atatgttttt caaacaacat tcctgtggag catatttagt ttgacagaga
    14941 ttgaccaact aatttttttt ttttaaatca tttcagaaaa tagcattcat gtattcagtt
    15001 gcaagtaaat ttcgtctttt cattgttttg ctttttgcta caaacattgc ttttggccag
    15061 caactcaaca aaaattatct gtcctacatc gaaaaatacc atcagttggc catcaaacaa
    15121 caaaaaaaac acggcattcc cgccagcatt atcctggctc agggtttgct ggaatcgtcg
    15181 gccggacaaa gccaactatc acaactctcg aacaaccatt tcgggatcaa atgcaattca
    15241 gattggaatg gagaaaccat ttcatgggac gatgacgaaa aaaatgaatg tttcagaaaa
    15301 tacaaaaatg ttgccgattc gtatgaagat cactcattgt ttctaaaaaa tcgtcctcgc
    15361 tatgctttcc ttttccaact agacaaaact gattatcaag gttgggctca tggactgaaa
    15421 aaagccggct atgctactga tccaacttat gcatacaaac taatttccct gatagaaaac
    15481 tacaacctga atagattcga caaacaaatt tttgataaca attatgccga aagaatggaa
    15541 attcaaatca acacttccat tccacaaacc attcaaaaag tgagcgatta cgtaacccac
    15601 aaaaagttta aaaacaatgg agtcccttat gtaatagctg ttccgtgcga tacttatgcc
    15661 aatattgctc aagaattcaa tttaagcgaa gaaaccctca gaagttacaa tgaagtcagc
    15721 gcaaccacag cattacaacc gggagaaaaa gtatatattc ggcctaaaaa gaacaaagct
    15781 tccaaaaatt atccggttca tgtggtacaa gccggagaat ccatgcacga cattgcacaa
    15841 ttttacggta taaaaaccga aaaactttat aaaatgaacc gcattccctt taccgaagga
    15901 gcacaagtag gacaagtact gaaattacgc taaaaaatgc tctttattta aaatcatgcg
    15961 aagaaaaaac acggaattat taagcgaaat cattcggcaa atgttgaaag accaacattt
    16021 ggaaaaaccg ttgtatgaaa aaagaattat tgacgcatgg acttctgttt tgggccctca
    16081 aataaaaaaa tataccggcc atttggaagt caaaaatgga attttatatg tcagcatcaa
    16141 ctctgccgtt ttgcgacacg atttattcct ctcaagagaa gaaataaaaa acgctttaaa
    16201 caaagaagtt gggcatgaag ttatcaacgc tatcgttttc cgttgataaa tatttctcta
    16261 taacatactg aatataagag atataatttt atccttccat aagaaaactt caaaaaagta
    16321 acatacaatg aaaaaaagtg ttacttttgc tcacttattc aaagcccatt aaagggtaaa
    16381 tttttatgtt taatcacaat aatttattgt tatgtcagct aagaaaaaca ttattgcatt
    16441 gcgccaaaag aaagcaatca tagaaaaagg cggcggtgaa aaagctattg aaaaacaatt
    16501 ggcaatgggt aaaatgacag cccgcgaccg catccgcgct ttgttggacg aaggctcatt
    16561 tcacgaatac gatttgtttg tgcagcacga agaaaaaaac ttcggaatgg acacaaaaga
    16621 tttgcccggt gatggagtta ttacaggaac tggatctatc aacggaaatc ctgtttgcat
    16681 ttatgctcaa gattttacgg ttatgggcgg ttctttagga attcaacatg ccagaaaaat
    16741 cacaaaaatc atggatcatg cgctgaaaat gaaaatgccg tgcatcggca tcaatgactc
    16801 gggaggtgca cgtattcagg aaggaattgg cgcattggcc ggttatggcg aaatttttta
    16861 tcgcaacact atggcttccg gtgtaattcc tcaaatttca gttattcttg gaccttgcgc
    16921 cggtggtgca gtttattctc cggcactgac cgattttgtg tttgtcgtag aaaatatttc
    16981 aaaaatgttt atcacgggtc ctaatgtgat tgaaaccgta ctgggagaaa aaatttcaca
    17041 ggaagatttg ggaggcgcca gagtacatgc cgaaatcaca ggtaatgctc acttttacgc
    17101 actgagtgaa aaggaatgtc tgcaacaaat caaggaactt atttcttaca ttcctcacaa
    17161 caacgaagaa aaagctgcca gagttgaacc caaaaagcca aaattcaaga aaaaaattga
    17221 aaatatcatt ccgacaaatc caaaattacc atacgatgta cgagaaataa tttacgcgct
    17281 ttcagatgat tctaaatttt ttgaagtaca tcaactttgg gcacccaata ttgtagtggg
    17341 atttgctcgt atagaaggag aaacggttgg ttttgttgcc aatcagccaa tgtatttggc
    17401 cggtgtactc gattgcgacg cttcagataa agctgcccgt tttattcgat tctgcgatgc
    17461 ttttaacata ccgctcatta ctttggaaga tattccaggc tatttgccgg gtgtagatca
    17521 ggaacatgca ggcgttattc gtcatggagc aaaagtgctt tatgcttatt cggaagcaac
    17581 tgttccaaaa atcaccgtca ttttgagaaa agcttatgga ggaggataca ttgccatgaa
    17641 ttccagacat ttgggcgccg attttacttt tgcttggcct gaagccgaaa ttgcagttat
    17701 gggaccggaa ggtgctgcca acattatttt caaaaaagaa atcatggaag ctgaagatcc
    17761 cgatgccatg cgccaattaa aagttaagga atacatcgaa aaatttgcaa atccatttgt
    17821 tgccgccggt aaaggttata ttgatgccgt tatcgaacct tcggaaactc gtgaactgct
    17881 tatacattcg ctaagtgtat caaggaaaaa atttgattat cgcccgacaa gaaaacacgg
    17941 aaatattcct ttataatttg gctcggcgaa aaatcagtat ttttcatctt tattttttgt
    18001 ttctgcaact aattaattat caaaattatg ataaaaaaca accgtttgtc ttgataatct
    18061 ttcagtaaaa agaaccaaaa aattatttga aaaaagccga cttatgccaa aaagctgatt
    18121 aacgagcttt ttgctcaact taaaaacata gtatttatga aaaaaaataa attatctata
    18181 caggaaaacc aagaattaat agattttgca gttactgcgc gaaaatataa aactcaacta
    18241 accaaaaaat tcttgaacag aaaaaaatgg gaaaatccga gtccttttga aatctattca
    18301 tttattccag gaacagtggt agacattttc gtaaacgaag gcgatattgt ggaagaagaa
    18361 acacccttaa tgacgctgga agccatgaaa atgcaaaata aaatagaaat gcctttcact
    18421 gcaaaaataa aaaaaattaa tgtgaagaaa ggtgagcaaa tacctaaaga ctttctgata
    18481 ttcgaattgg aaccggtcga ataacggcaa tttcaggaaa aaacatcctg tgtttaaacc
    18541 gaaaaaaatc tcttcgtcaa taacgacaac aatgtcgaaa aaagcggaga gattttttta
    18601 catattgtgg tagaaacaat tgaataacaa ttaaaaaaac aactttcagc cctcgcttgc
    18661 ccaaaagttt gcttttcatc aaaacttcaa attgcctatc ttcataaaat attatacttt
    18721 tgtactctca acggaagtat tttcttatcc aactgttgat tggggaaaat ccaaaatttt
    18781 cattaactga aaaaccaact tttacttatg aatcagaccg ataaacaatt tgaccagatt
    18841 gctggcatct gccgcaacat tttcattcag aagctgaaag attatggcgc ttcatggcgt
    18901 ttgttgcgtc caacttcggt taccgatcag ctctatatca aagccaaccg catcaggaac
    18961 attcaggtaa aaggtttttc gaaagtcgat gaaggcattc agtcagattt aatcggcatt
    19021 gtaaattacg caatcatcgg tttgattcaa ttggaaaaag gatttgccga aaaacctgat
    19081 ctttccgtcg aagaaacgat tgctctgtac gacaaataca tgcaacaggc taaagaacta
    19141 atgatggaca aaaatcatga ttacgacgaa gcatggagac acatgcgaat cgaatcgatg
    19201 gtagatttga tattgatgaa aattgaaaga accaaacaaa tcgaagaaaa tgatggaaaa
    19261 actttgattt cggaaggcat tgatgccaat tattttgata tgatcaatta tgccattttc
    19321 gccctaatca aaatggatga aaacgactgt caacaaaaaa acgaaaaaat ttaaagccac
    19381 tgaagcaatt aattaaatct catgtcgtca gatatttata aaaacaaaaa aatggctttt
    19441 cctgccgaac aaaagggaaa aaaaattcta tttgttcgaa tatgcagcat ccttttggga
    19501 gcgctttttg tattttcagg cttcgtgaaa gccatcgatc cgctgggcac tacttataaa
    19561 attgaagatt atttgaatgc ttttggcggt ccttttcccc aattggtttt tttggctttt
    19621 ccggctgctg tttttctttc tactttggag ttggtgatgg gactgaatct tttgctccga
    19681 atttatgtac gattcaccac atgggtagcc gctgtattcc tgttttttat gaccttgctg
    19741 actttttaca tcgctttaaa aaatcccgtg acggactgtg gctgctttgg cgaggcaatt
    19801 gtccttacga attggcaaac ttttattaaa aatatttttt tcattgctct gtcgactttt
    19861 ctgcttatca accgcaaaca tctttatccg cttgttcttc cttcattaga agccattttc
    19921 actgtttcat tcgttatagt aggcgttggc atttcggttt attcttattt tcacttgcct
    19981 atcatggatt ttcgtcccta caaagtgggt gtaaatattc cggaagccat gaaaattccg
    20041 gaaggagcca aacccgacgt atatgaaact acattcatct acgaaaaaga ggggaaacaa
    20101 aaggaattca atctggaaaa ctacccgaaa aacgacaaaa gctggaaatt tgtagaccaa
    20161 aaaaccaaac tgatttcgaa aggctatcga cctccgattc acgattttac tattctcaat
    20221 aaagacaatg aagatatttc tgacattatt ctttctcaac ccgattcaac ttttctgctc
    20281 atcatgtacg acatcaacaa aacttctcag gaaggggcga aaaaagcaga aagaattttt
    20341 caaaaaactc agcaaaacgg gcattcgttt tatgccatta cagcttcaac cgataacgac
    20401 attcaaaaat ttacagaacg tacaaaaatt tcatttcctt tctacaaagc cgatcctatc
    20461 atgctgaaaa ccgtcattcg cgccaatccg ggtttggtac tgataaaaga tggaacgatt
    20521 ttgggaaaat ggaattggcg agattttcca gaaaactttt attaagcgtc aaaaaagaat
    20581 aaatttcttc agacaaactt ctacaaataa actaaattta tcaaactcaa taaaaaaaca
    20641 gtaaatatga gaaaaaacat tgtagccgga aattggaaaa tgaataaaac cttaccggaa
    20701 ggtttggcat tggcaacaga actggacaaa atgctttcga attaccagcc caagtgtgaa
    20761 gtaattattg ctccgccgtt tatccatttg gcaagcataa cagcaactat tgatacaaaa
    20821 aaaattggcg tttctgcaca aaattgtgcc gataaggaat cgggagccta tacgggagaa
    20881 gtgagtgcag caatggtaaa atctaccggt gccaactatg ttattttagg tcattccgaa
    20941 cgcagaacat attatggtga aactcccgtc ctactgaaag aaaaagtgaa tctggcattg
    21001 aaaaacgaac ttacacccat tttttgcatt ggtgaagtac tggccgaaag agaatcggga
    21061 atttattttg acgtagtgaa atctcaaatt gaaggctcat tgttccacct ttctgcagaa
    21121 gatttttcaa aaataatcct tgcttacgaa cccgtttggg caattggaac cgggaaaact
    21181 gccacacctc agcaggcaca agaaatgcat gctttcatac gccaaatcat caccgaaaaa
    21241 tatggaaaaa acatagccga aaacacttcc attctttacg gtggcagctg caatccgggc
    21301 aatgctaaag aattgtttgc taacgaagat gtggacggcg gactgattgg tggagcttct
    21361 ttaaaagctg ccgatttcaa agctattatc gacgcatttt aagcactgaa cattaacgaa
    21421 acttaccaaa tatgatcatg tcacactaaa aaatggaatt cggtaaaatt ccactgagtg
    21481 gtatttaggc tctacgttag caggttttag tccaattcaa taaaccttca atttttcatc
    21541 agaatataat ttatttttaa aggtctgatg gaacccacat gtctgaatat tatattcatt
    21601 cgtgtttgtg tatgcaatac atgtgggttt cttatattga aaatgtgaat ggttataaaa
    21661 ttggctattc tacaaatacg gctcacctgc aaaaacatgt gtttgtagaa aaagaaaagt
    21721 atctttgtga aaa
//
LOCUS       DAOTAX010000031        21428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2011_length_21428_cov_30.325598, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000031 DAOTAX010000000
VERSION     DAOTAX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21428
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2011_length_21428_cov_30.325598"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..167
                     /locus_tag="PLM04_04835"
     tRNA            95..167
                     /locus_tag="PLM04_04835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Gly,seq:tcc)
     gene            257..328
                     /locus_tag="PLM04_04840"
     tRNA            257..328
                     /locus_tag="PLM04_04840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Thr,seq:ggt)
     gene            400..1587
                     /gene="tuf"
                     /locus_tag="PLM04_04845"
     CDS             400..1587
                     /gene="tuf"
                     /locus_tag="PLM04_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HON02435.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGLAEM
                     RSFDSIDNAPEEKERGITINTSHVEYQTAARHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRLVVFINKCDLVDDEEMLDLVEMEMRELLSF
                     YDFDGDNTPVIRGSALGALDGDPKWEAKIVELMDAVDNWIQLPQRDVDKPFLMPVEDV
                     FSITGRGTVATGRIETGIVKVGDEVQIIGLGHEAKKTVVTGVEMFRKILDQGQAGDNV
                     GLLLRGIDKDEIKRGMVITHPGAVTPHKKFKAAVYILKKEEGGRHTPFHDHYRPQFYI
                     RTMDVTGEITLPEGVEMVMPGDNIEFTVELIYPVACSVGLRFAIREGGRTVGSGQITE
                     ILD"
     gene            1714..1790
                     /locus_tag="PLM04_04850"
     tRNA            1714..1790
                     /locus_tag="PLM04_04850"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1748..1750,aa:Trp,seq:cca)
     gene            1806..1997
                     /gene="secE"
                     /locus_tag="PLM04_04855"
     CDS             1806..1997
                     /gene="secE"
                     /locus_tag="PLM04_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HON02436.1"
                     /translation="MKIINYIKECYSELVHKVSWPSLSELTNSAIVVLIASIILALIV
                     WVMDLSFESIMTFIYEKIF"
     gene            2023..2571
                     /gene="nusG"
                     /locus_tag="PLM04_04860"
     CDS             2023..2571
                     /gene="nusG"
                     /locus_tag="PLM04_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HON02437.1"
                     /translation="MSDTTNFKWYVLRAISGKENKVKEYIETEIKNSDLGQYVRQVLI
                     PTEKVFQIRNGKKIAKERNCMPGYVLIEANLMGEALHRVRETPNVLGFLGEKNNIPTP
                     IRTSEINRILGTMDELQESEEEMITPFMVGENVKVIFGPFSGFSGTIEEVNTEKKKLK
                     VMVMIFGRKTPLELAFTQVEKE"
     gene            2625..3065
                     /gene="rplK"
                     /locus_tag="PLM04_04865"
     CDS             2625..3065
                     /gene="rplK"
                     /locus_tag="PLM04_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HON02438.1"
                     /translation="MAKEIAGVIKLQIKGGAANPSPPVGPALGSKGINIMEFCKQFNA
                     RTQDKAGKILPVVITYYTDKSFDFVVKTPPVAIQLLELTKLKSGSPEPNRKKVAEISW
                     DQVKEIAQDKMVDMNCFELESAMRMVAGTARSMGITVKGTFPGK"
     gene            3087..3782
                     /gene="rplA"
                     /locus_tag="PLM04_04870"
     CDS             3087..3782
                     /gene="rplA"
                     /locus_tag="PLM04_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HON02439.1"
                     /translation="MSTLTKNRKYALEKIEAGKAYPLIEAAKLVKEVTTTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPNGTGKQVKVLALCPPELEAAAKEAGADYVGLDEYIE
                     KIKGGWTDVDVIITTPAVMGKLGALGRILGPRGLMPNPKSGTVTNDVAKAIKEVKQGK
                     IDFKVDKFGIVHASIGKVSFTAEQIAENAQEFISTLIKLKPSTAKGTYIKSIYLSSTM
                     SPGIKVDPKSIEI"
     gene            3794..4309
                     /gene="rplJ"
                     /locus_tag="PLM04_04875"
     CDS             3794..4309
                     /gene="rplJ"
                     /locus_tag="PLM04_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HON02440.1"
                     /translation="MKKEDKSTIIDQIESVLSEYPHFYLTDIGGLNAAQTSELRKLCY
                     KEKVKLVVVKNTLLKKALDRSSVDFSELYPTLKGETALMLSDMANVPAKLIKNFSAKN
                     KKPVLKAAYVEESFYIGENQLDTLVNVKSKNELIADLIALLQSPAKNVVSALQSGANT
                     IHGVLQTLSEK"
     gene            4348..4725
                     /gene="rplL"
                     /locus_tag="PLM04_04880"
     CDS             4348..4725
                     /gene="rplL"
                     /locus_tag="PLM04_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HON02441.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAQILKNDYGIEPAAAAVAVAAG
                     PSAAAAEAVEEKTAFDVVLKSPGANKLAIVKLVKELTGLGLKDAKDLVDAAPSVIKEG
                     ISKDEAEALKNQLVEGGAEVEIK"
     assembly_gap    4866..4965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4966..>6176
                     /gene="rpoB"
                     /locus_tag="PLM04_04885"
     CDS             <4966..>6176
                     /gene="rpoB"
                     /locus_tag="PLM04_04885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564161.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HON02442.1"
                     /translation="GQSIHANGTKLYSARIIPFRGSWIEFATDINNVMYAYIDRKKKL
                     PVTTLLRAIGFESDKDILEIFNLAEEIKVNKSNLKKAVGSKLAARVLKTWLEDFVDED
                     TGEVVTIERNEIIIDRETVLENEHIDLILESGAQTILIHKEEANQNDYSIIYNTLQKD
                     PSNSEKEAVLYIYRQLRNAEPPDDATAREVINNLFFSEKRYDLGEVGRFRINQKLNLT
                     TSMEVKVLTKEDIIEIIKYLIDLVNSKAEVDDIDHLSNRRVRTVGEQLYNQFSVGLAR
                     MARTIRERMNVRDNEVFTPGDLINAKSISSVINSFFGTNALSQFMDQQNPLAEITHKR
                     RMSALGPGGLSRERAGFEVRDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINELGF
                     IETPYRKVNNGV"
     assembly_gap    6177..6276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6277..7947
                     /gene="rpoB"
                     /locus_tag="PLM04_04890"
     CDS             <6277..7947
                     /gene="rpoB"
                     /locus_tag="PLM04_04890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HON02443.1"
                     /translation="VKEYKLPKYQKTNQNTTIDLRPIVRRGDRVHAGMIITEGYGSES
                     GELALGKNLKVAFMPWKGYNFEDAIVINEKVVREDVFTSIHVVEQLLDVRETKRGTEE
                     FTSDIPNVSEDATRNLDENGIIRVGARVEPGDIIIGKITPKGESDPSPEEKLLRAIFG
                     DKAGDVKDASLKATPSLSGVVIGKRLFSKAQKNRKSKLADKAVLPKLDEEFETQANAL
                     RETLIEKLMKILTNKTSTGVKDFLGTDIIPRNSKFTVERLREIDYTTVQLGKWTNDAH
                     TNDMVNSLITNFLRKFKELDAQLKRQKFNITIGDDLPSGIMQMAKVYIAKKRKIRVGD
                     KMAGRHGNKGIVSRIVREEDMPFLEDGTPVDIVLNPLGVPSRMNLGQIFESVLGWAGK
                     ELGVRFATPIFDGATLNDLNAWTDKAGLPRAGKTYLYDGGTGERFDQPATVGVIYMMK
                     LGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAANILREILT
                     VKSDDVNGRARTYEAIVKGDPMPTPGIPESLNVLLHELRGLCLSINLE"
     gene            8029..12327
                     /gene="rpoC"
                     /locus_tag="PLM04_04895"
     CDS             8029..12327
                     /gene="rpoC"
                     /locus_tag="PLM04_04895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HON02444.1"
                     /translation="MAFKTDNKIKSNFSKISIGLASPEEILKLSHGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRTLPSKIGYLLGMSSKKLESIIYYEKYVIIQGGILENGDNIATGELL
                     TEEEYLDILDSLPKDNQQLEDTDPNKFIAKMGAEAIYDLLSRIDLDALSYELRHKANT
                     DTSQQRKAEALKRLQVVEAFRASKNRNRPEWMIVKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKASAVKTEAN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FIIRKLIERGIVKTVKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKLIEGMAIQLHPLACTAFNADFDGDQMAVHLPLSNEAVLEAQLLMLASHNILNPAN
                     GAPITVPSQDMVLGLYYITKPRPNALGEGLIFYSPEEAEIAYNEHKVDIHARVKVRTK
                     DLDENGNSVEKLIETTVGRIIFNKYVPEEVGYINELLSKKSLRDIIGRVIETCGMART
                     AHFLDDIKELGYNMAFRGGLSFNLDDVIIPKDKEKLIEEGNREVEEILNNYNMGFITY
                     NERYNQIIDTWTHVNSKLSDVLMKELSSDNDGFNSVYMMLDSGARGSKEQIRQLSGMR
                     GLMAKPQKSVSEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVTITEEDCGTLRGLIATEMKNNEEVISSLYERILGRVSVHDIYHPLTG
                     ELIVSSGEEITESLAKKIQDSPIEQVEIRSVLTCESKKGVCAKCYGRNLATGRMVNIG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIASNIAAESKIVLRYDGRLEFDELRTVQPSD
                     DQSYRVVVSRLAEMRVVDPNTGIVLTTMNIPYGSKLYVKEGDICTKGQLICEWDPFNA
                     VIIAENEGKIEFEDVVENVTFKVETDEATGLREKIIIESKDRNKVPTAHILDKDGNIL
                     KTYNLPVGAHIVFENGDYIKTGQILVKIPRAVGKAGDITGGLPRVTELFEARNPSNPA
                     IVAEIDGEVTFGKIKRGNRELSITSKTGEVKKYLIPLSKQILVQENDFVRAGTPLSDG
                     SITPTDILQIKGPTAVQDYIVNEVQDVYRLQGVKINDKHFEIIVRQMMRKVEIIEPGD
                     TRFLEKQVVDKNDFMEENDRIWGKKVIVDAGDSPTLKPGQIITPRKLRDENSMLKRKD
                     LRPVQARDAIPATSNQILQGITRAALQTNSFMSAASFQETTKVLNEAAINAKVDKLEG
                     LKENVICGHLIPAGTGQRDFEKIVVYSREEYEKKVFARKSLIDVEEGEVVEENN"
     gene            complement(12418..13248)
                     /locus_tag="PLM04_04900"
     CDS             complement(12418..13248)
                     /locus_tag="PLM04_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HON02445.1"
                     /translation="MNSKPDFLIDIEKVVKEKAPNTFIPSFLVSYLRKIVHEKEINEL
                     LTTYSHQKNVDFLESAVKYFQVNVSIKNRENLPHDDKLYIFAGNHPLGGMDAIMTGSL
                     LGREYDGKIRFMANDMLMFIEPLKDLFIPVNKVGSQGKNHVRELNDFFSSDCHLITYP
                     AGKCSRKSNGKIQDEAWNKNFVVKAIQHQRDIVPIYFDGKNSNFFYNLANLRKFFHIK
                     FNIEMLYLSDEMFKQKGKSFSLVIGEPIRWQTFDRSKTYGKWAQWVKEKVYALSEKTF
                     "
     gene            13588..14037
                     /locus_tag="PLM04_04905"
     CDS             13588..14037
                     /locus_tag="PLM04_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HON02446.1"
                     /translation="MSTFIGKYEAKADVKGRIFIPSAYRKILGVDDREQIIMRKDAGN
                     ECIVFYPESIWNQKVENLKSKLDEWNPSDQLLLMQFVADAEWLDIDAQGRVLISKKHL
                     QTIGVENAEVLFVGMIDRFALWSKSRYEQMQLSAENFAALLKEKMSK"
     gene            14052..14972
                     /gene="rsmH"
                     /locus_tag="PLM04_04910"
     CDS             14052..14972
                     /gene="rsmH"
                     /locus_tag="PLM04_04910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HON02447.1"
                     /translation="MSEKEYHIPVMLKETLQGLVLNPDGIYVDVTFGGGGHSRGILDL
                     LSKKGKLIAFDQDQDACQNIFEDDRFLFVQSNFCYLKNFLRYHGISLVDGILADLGVS
                     SHHFDESERGFSFRLDGELDMRMNRKTELTAATVLNTYTEEQLADVFYHYGELSNARK
                     IAATIVKARDREPFQKILPFVEMMKRFFPRQKEKKGLACVFQALRIEVNHELEALQEF
                     LQQTPDVLKPGGRLAVLTYHSLEDRMVKNFMKTGNFEGILNKDFFGNIIAPFKPISGK
                     AITPQLSEIEKNPRARSAKLRIAELIDKTN"
     gene            15020..15400
                     /locus_tag="PLM04_04915"
     CDS             15020..15400
                     /locus_tag="PLM04_04915"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HON02448.1"
                     /translation="MSWFSKIIENIKGSEDFSELKSSSFRDILNGNIFTKRFFIKQIG
                     LLIMIVVLFIFYIGNRYEYESELTYQNKLKKEVQDKKYESLSISAELMQVSRQSNVEK
                     MLKERGIELHEPVSPLVVLDDSIQ"
     gene            15409..17550
                     /locus_tag="PLM04_04920"
     CDS             15409..17550
                     /locus_tag="PLM04_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HON02449.1"
                     /translation="MADQHKNIVFRFGIVYVFIVVAFLLVIYKIITIQFFERENWLAL
                     AAKNVKTNIEVKANRGNIFSSDGRLMASSIPTYYIYMDLRVPALQENNGQLFHENIDS
                     LSLCLSKFFKDKSQAEYKKDITEAYNKKLGAFRIYPKKISYSQLKELKTFPLFRLGKN
                     KSGLITKEQFRRVKPFGSLASRTIGDIYADETKGGKNGLELKFDSLLRGTPGIAIRQK
                     VANTWQETVQVEPIDGIDVITTIDVDIQDIAEKALLDSLRSFDAQEGYVIVMEVQSGE
                     VKAIVNMQKNENGTYSENRNGAVSDMVEPGSTFKVVSLMAALDDGKVKVTDSIDTGNG
                     IFQFANAFMKDHNYNRGGFHKITIAEAIHASSNIGISRMIVNAYGNNPAAYVDKLYKM
                     KVNEPMNLEIPGTAAPNIRHPLDKSKYWSKTTLPWMSIGYETQIPPIYTLAFYNSIAN
                     DGKLIRPYFVKALSKNGQILKTFQTETINEQICKPSVLQTIRETLLGVVEGRLGTAKN
                     VRSKYVRIAGKTGTAQISKGALGYRSGGKSHQVSFCGYFPAEHPLYTCIVVIREPRIG
                     YPSGGKMSGSVFKDIAERIMFLRSTLKPEHFVPDTMRNVPKVPYALWGSFSDLEKVTS
                     EMRLPISGKAGSFVNVLPDEKNNGLEISKKTVYRQLMPDLKGMGAKDAVFLAEKLGLK
                     VMISGRGKLVSQSISPGTKITKGQILSLHFQ"
     gene            17568..19013
                     /locus_tag="PLM04_04925"
     CDS             17568..19013
                     /locus_tag="PLM04_04925"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HON02450.1"
                     /translation="MILKVALEKIFPTEIIGNTEMEISGVQSDSRKVDKGNIFVATRG
                     TNVDGHNYISPAIEKGASAIVCETFPETIQQGIVYIRVENSLEALGLLACAWYGNPSE
                     KLTLVGVTGTNGKTTIATLLYQMFRQLGYKAGLLSTVRNYVVDEPVEATHTTPDPLEL
                     NHLLAQMVEAGCQYAFMEVSSHAIDQRRIAGLLFKGGIFTNITRDHLDYHLTFENYLK
                     AKKKFFDDLPENAFALTNLDDKNGLVMLQNTKAKKYTYSLRSMADFKTRILEQDFEGM
                     LLEMNGKQVHVSFVGRFNASNLTAVFGAAVLMGEEETEVLRILSLLHPVSGRLETLHA
                     PSGYTVIVDYAHTPDALNNVISTIHQVLQNKGRLITVVGCGGNRDKGKRPLMAKEAVT
                     GSWKTILTSDNPRFEDPMDILNDMIAGLTDEERKTSLVIPDRRQAIHTACSLAQPGDV
                     VLIAGKGHEDYQIIKGEKHHFDDREVVKECF"
     gene            19108..20370
                     /gene="mraY"
                     /locus_tag="PLM04_04930"
     CDS             19108..20370
                     /gene="mraY"
                     /locus_tag="PLM04_04930"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HON02451.1"
                     /translation="MFYHLFRYLDKFYDIPGAGMFNYISFKTGLSFIFALLLSTLFGR
                     RIIDFLQKKQIGETIRDLGLEGQLSKKGTPTMGGIIIINAILIPTLLFADLTNIYIIL
                     MLITTVWMGAIGFVDDYIKVFLKNKEGLSGKAKIVGQVGLGLIVGLVMYFSPDVVMRE
                     NTEIRGELRVEDVVYSKKDIKATRTTIPFVKNNDLDYSNAFKNAGKNAQKYGWILFVL
                     VAIFIVTAVSNGANLTDGLDGLSAGSSAIMGGALGVLAYLSGNINYSGYLNIMYIPGT
                     GELLVFAGAFLGATIGFLWYNSFPAQIFMGDTGSLTLGGIIAVFAIAIRKELLLPILC
                     GVFLAESLSVICQVSYFKYTKKRYGVGKRIFKMTPLHHHFQKPGNAGIEALIQRPLQA
                     VPEPKIVVRFWIVSMILAALTIITLKIR"
     gene            20410..>21428
                     /gene="murD"
                     /locus_tag="PLM04_04935"
     CDS             20410..>21428
                     /gene="murD"
                     /locus_tag="PLM04_04935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HON02452.1"
                     /translation="MKRIVVLGAGESGAGAAVLAKQQGFDVFVSDLSEIKENYKELLN
                     QYQIEWEEKQHTEALILNADEVIKSPGIPDKAPIIKNLKEKNIPIISEIEFAGRFTNA
                     KMICITGSNGKTTTTMLTYHILKNAGYNVGLAGNVGQSLAYQVATSSRDYYVVEISSF
                     QLDGMYDFKADVAILLNITPDHLDRYEYNFQNYVDSKFRIIRNQTSEDAFIFWDDDPV
                     IQSEIAKRSILAAKYPFSLKKKEHCKAYIENNNLIIETSNNPLIMPVKELSLKGIHNT
                     YNMMAAGLAASAVNVKKEFIRQSLQDFQGVEHRLEYVATVKNVTFINDSKATNVNSCW
                     YALES"
BASE COUNT         6738 a         3848 c         4652 g         5990 t
ORIGIN      
        1 tgactgtaac tcagctgtct ttcgacttcg gtggttcgaa tccatcactg cccacgataa
       61 aacagaaatt ttttttctga tttattgaaa ttttgcggaa gtagctcagt cgatagagca
      121 ttagccttcc aagctgaggg tcgcgggttt gagtcccgtc ttccgctctg aaaatctgaa
      181 ggaaacaaac aacggttcac atgaaacagg accgttgtat tccttttttt acagaaggga
      241 actgtaaaaa ttgcacgctg ttgtagctca gaggtagagc acttccttgg taaggaagag
      301 gtcgcgggtt caattcccat caacagctct atgtttttct tgaaacaaag cttttataaa
      361 acataaatta ctaattatta aaaaaactat tctaagatta tggcaaaaga aaaatttgac
      421 aggtcaaaac cacatgtcaa cattggaaca atcggacatg ttgaccatgg taaaactact
      481 ttgaccgccg ctattaccaa agtgcttgct gaaaaaggac ttgctgaaat gcgatctttc
      541 gattctatcg ataatgcacc agaagaaaag gaacgtggaa tcaccattaa tacttcgcac
      601 gtagaatatc aaacagctgc ccgccactac gctcacgttg actgtccggg acacgccgac
      661 tacgttaaaa acatggttac gggtgcagcc cagatggatg gagctattat tgtggttgca
      721 gctactgatg gtccaatgcc acagactcgc gaacacattc ttttggctcg tcaggttaac
      781 gtaccaagat tggttgtgtt tatcaacaaa tgcgatttgg ttgatgacga agaaatgctc
      841 gaccttgttg aaatggaaat gcgcgaactt ctttcattct acgatttcga tggcgacaat
      901 actccggtta ttcgtggttc agctttgggc gcattggatg gcgatcctaa atgggaagct
      961 aaaattgttg agttaatgga tgctgttgac aactggattc aattacctca acgtgatgtt
     1021 gacaaaccat tcttgatgcc tgtagaagac gtattctcca tcacaggacg tggaactgtt
     1081 gctacaggaa gaattgaaac cggaattgtg aaagttggtg acgaagttca gattatcgga
     1141 ttgggacacg aagcaaagaa aaccgttgta acgggtgtag aaatgttccg taagattttg
     1201 gatcaaggtc aggccggcga taacgtaggt ttacttttga gaggtatcga taaggacgaa
     1261 atcaaacgag gtatggttat tacccacccg ggagccgtta cacctcacaa gaaattcaaa
     1321 gctgccgttt atattctgaa gaaagaggaa ggtggacgtc atacgccttt ccacgatcac
     1381 tatcgtccgc agttctatat tcgtacaatg gacgttaccg gtgaaattac tcttccggaa
     1441 ggagtggaaa tggtaatgcc cggcgacaat atcgaattta ctgtagaact gatttatccg
     1501 gtagcttgca gcgtaggttt gcgttttgct attcgtgaag gcggacgtac ggtaggttct
     1561 ggtcagatta cagaaatttt ggattaattt gacagacgga tgtcgttcac ttttttggac
     1621 atcactaaaa aataaacatg aattaatctt tttaagctct tttcctttta cagactttca
     1681 aatcattaaa gtctgtaaaa ggaaaaatta cctacgggcg tagctcagtt tggtagagca
     1741 ctggtctcca aaaccaggtg tcgggagttc gagtctctct gcccgtgcca atttgtacaa
     1801 aaaacatgaa aataattaac tatatcaaag agtgctattc agaactggtt cataaagttt
     1861 cttggccttc gctctcggaa cttaccaaca gcgcaattgt tgtattgatt gcttccatca
     1921 tccttgcgtt gattgtctgg gttatggatt taagttttga atctatcatg acgttcattt
     1981 acgaaaaaat cttttaatct ttaatcagtg gaggtcgaga aagtgtctga tacgacaaat
     2041 tttaaatggt atgtactccg tgccattagt gggaaagaaa acaaggtaaa ggaatatata
     2101 gaaacagaaa tcaaaaattc tgaccttgga cagtatgtac gtcaggtttt gatccctact
     2161 gaaaaagttt ttcagattcg taacggcaaa aaaattgcta aagaaaggaa ttgtatgccg
     2221 ggttacgttt tgatagaagc aaatcttatg ggtgaagctc ttcatcgtgt tcgcgaaaca
     2281 cccaatgttt tggggttttt aggcgaaaaa aacaacatcc ctacgcccat ccgcacttcc
     2341 gaaataaata gaattttagg gacaatggat gaattgcagg aaagtgaaga agaaatgata
     2401 actcctttca tggtaggaga aaatgtaaaa gtcatcttcg gacctttttc gggatttagt
     2461 ggaacgattg aagaggtaaa caccgaaaag aaaaaactca aagttatggt tatgatattc
     2521 ggtcggaaaa ctccgctcga attagcgttt actcaagtag aaaaggaata gttggctcgg
     2581 ttacaagtcg attatttcga taacaatcaa aaatcaaaac aattatggca aaagagattg
     2641 ctggagtaat taaattgcag ataaaaggag gtgctgcaaa tccatctcct cccgtaggac
     2701 ctgctttagg ttctaaaggg atcaacatta tggagttctg taaacaattt aatgccagga
     2761 ctcaggacaa agccggtaaa atcttacccg ttgtcattac ctattatacg gacaaatctt
     2821 tcgattttgt tgttaaaacg cctccggtag ccattcaatt gctcgaactt accaaactca
     2881 aaagtggatc tcctgaaccc aaccgtaaaa aggttgccga aatttcttgg gatcaggtga
     2941 aggaaattgc acaggataaa atggttgaca tgaactgttt cgaattggaa tcagccatga
     3001 gaatggtagc tgggacagca agaagtatgg gtattaccgt gaagggtact ttcccaggta
     3061 aataacaatt taacacttca attacaatga gtacattaac aaaaaatcga aaatacgctt
     3121 tagaaaaaat tgaagctgga aaggcatatc cactcattga agctgcaaag ttagtaaaag
     3181 aagttactac tactaaattt gatgcttcgg tggatatcga tgtgcgttta ggtgttgatc
     3241 cccgtaaggc caatcaaatg gtacgtggtg tcgtttccct tcccaatggg acaggaaaac
     3301 aggtgaaagt acttgctttg tgccctcctg aattggaagc tgctgctaaa gaagccggcg
     3361 ccgattacgt tggactggac gaatacatcg aaaaaattaa aggtggctgg acagatgttg
     3421 atgtcatcat tactacacct gctgtaatgg ggaaattggg agctcttgga agaatacttg
     3481 gcccccgcgg tttaatgcca aaccctaaaa gcggtacagt aacgaatgat gttgccaagg
     3541 ctattaagga agttaaacag ggaaaaatag atttcaaagt cgacaagttc ggaattgttc
     3601 atgcttccat cggaaaagtt tcctttactg cagaacaaat cgccgaaaat gcacaggaat
     3661 ttatttctac gctgataaaa ctgaaacctt ctacagccaa aggaacttac attaaaagca
     3721 tttatctttc tagtacgatg agtccgggta taaaagtaga tcccaaatca attgaaattt
     3781 aaagattaaa cttatgaaga aggaagataa aagcacaatc attgatcaga ttgaatcggt
     3841 tctgagcgag tatcctcatt tttaccttac tgacataggc ggtttaaatg ccgctcaaac
     3901 cagtgagttg agaaaacttt gctacaagga aaaggtgaaa ctggtagtgg ttaaaaatac
     3961 tttgttgaaa aaagcactgg atcgctcatc ggttgatttc agtgaactgt atccaacttt
     4021 aaagggagaa actgctctca tgctttccga tatggctaat gttccggcca aactgataaa
     4081 aaatttcagt gccaagaaca aaaaacctgt tttaaaggct gcttatgttg aagaaagttt
     4141 ttacattggc gaaaatcaac tcgatacact tgtcaatgtg aagagcaaga atgaactgat
     4201 tgccgatttg attgctttgt tgcaatctcc ggctaaaaat gtcgtttcag ctctacaatc
     4261 aggagctaat actattcatg gtgttttaca aacactttct gaaaaataat ttttttaaat
     4321 taaaaacaaa tcaaaaaata gaataaaatg gcagatttga aagcttttgc agaacaattg
     4381 gttaacttga cagtaaaaga agtcaacgag ttagcacaga ttttaaaaaa tgattatggt
     4441 atcgaaccag ctgctgcagc tgttgctgtc gctgcaggcc catcagctgc tgcagcggaa
     4501 gcagttgaag aaaaaacagc ttttgatgta gtattgaaat cgccgggtgc caacaaactg
     4561 gcaattgtaa aattagtaaa agaacttacc ggtttgggac ttaaagatgc caaagattta
     4621 gttgacgctg ctccttctgt tataaaggaa ggtatttcga aagatgaagc tgaagcctta
     4681 aaaaatcagt tggttgaagg cggagctgaa gtcgaaatta aataatgtca gctgaatatc
     4741 agtaacttgg tttagaacct ttcgcaacgg gaggttctaa acctttttgt ctatacaata
     4801 tagtgagttc attgaacccc aataacctct ttgaaaatgt cttcatctac taacaatcaa
     4861 agaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggaca aagtattcat
     4981 gccaacggaa caaaattgta ttcagccaga attattccat ttcgtggctc atggatagaa
     5041 ttcgctaccg acattaacaa tgtcatgtat gcctatatcg acagaaagaa aaaattaccc
     5101 gtaaccacgt tgcttagagc cattgggttt gaaagtgata aggatatttt ggaaattttt
     5161 aatctggctg aagaaatcaa agttaataaa tccaatctga aaaaggctgt gggcagtaaa
     5221 cttgccgccc gtgtgctgaa aacatggttg gaagatttcg tggatgaaga taccggtgaa
     5281 gtagtaacca ttgaacgtaa cgaaatcatt attgaccgtg aaactgtatt ggaaaatgag
     5341 cacatcgatt taattctcga atcgggagct caaaccatac tgatacataa agaagaagcc
     5401 aatcagaacg attacagcat tatttacaat accttgcaaa aggatccaag caattcggaa
     5461 aaagaagctg tactttatat ttacaggcaa ttgagaaatg ctgaaccacc agatgatgct
     5521 actgcccgtg aagtaatcaa caatctgttc ttttcggaaa aaagatacga tttgggcgaa
     5581 gttggacgtt ttcgcatcaa tcaaaagctg aacctcacta caagtatgga ggtgaaagtg
     5641 ctcaccaaag aagatattat tgaaattata aaatatctga tcgatttggt gaattctaag
     5701 gctgaagtgg acgatatcga tcacctgagc aacagaaggg tcagaacagt aggtgaacaa
     5761 ttatacaatc aatttagcgt tggattggca cgtatggcac gtaccattcg ggaacgaatg
     5821 aatgttcgcg ataatgaagt ttttactccc ggcgatctga tcaatgccaa gagtatttca
     5881 tcggttatta attcattctt tggaaccaat gctttatcgc aattcatgga tcagcagaat
     5941 ccgcttgctg aaattaccca caagcgaagg atgtcggctt tggggcccgg cggtttgtct
     6001 cgcgaaagag ccggttttga agtgcgtgac gttcattata cccattacgg tcgtctgtgt
     6061 ccaatagaaa ctcctgaagg tccaaatatc ggtcttatat catctttgtg tgtgtatgca
     6121 aaaatcaatg agctcggttt tatagaaact ccttaccgta aagttaataa tggagtnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtta aggaatataa acttcctaaa
     6301 tatcaaaaga caaatcagaa taccaccatt gaccttcgtc caattgttcg caggggagac
     6361 cgtgtacacg caggaatgat tatcaccgaa ggctatggca gcgaaagtgg agagttggct
     6421 ttgggcaaaa acttgaaagt tgcttttatg ccgtggaaag gatacaattt cgaggatgct
     6481 attgtgataa acgaaaaggt ggttcgagaa gatgtcttta cttccattca cgtagtggaa
     6541 caattgttgg acgttagaga aaccaagcgt ggcactgagg aatttacttc cgacataccc
     6601 aatgtgagtg aagatgctac cagaaatctt gacgaaaacg ggattattcg agttggtgca
     6661 cgcgtagaac ccggtgatat aattatcgga aaaattacac caaaaggaga atccgatcct
     6721 tctcccgaag aaaagttgct tcgtgcaata ttcggcgata aggcaggcga tgtaaaagat
     6781 gcttctctga aagccacacc atcgttgtcg ggggtagtca ttggaaaaag attattctcc
     6841 aaagctcaga aaaatcgtaa atcaaaatta gctgacaagg cagttcttcc aaaattggac
     6901 gaagagtttg aaacacaagc caatgcgttg cgtgaaactt tgatagaaaa gttgatgaaa
     6961 attttgacca acaagacttc aaccggtgtg aaggactttt tgggtaccga tattatacca
     7021 cgtaacagta aatttactgt tgagcggttg cgcgaaatag attatactac ggttcagctt
     7081 ggaaaatgga caaacgacgc tcatacaaat gacatggtga acagtttgat taccaatttc
     7141 ttaagaaaat ttaaagaatt ggatgcgcag ctgaaaaggc aaaaattcaa cattaccatt
     7201 ggagatgatt tgccaagtgg cattatgcaa atggccaaag tttatatagc caaaaaacgt
     7261 aaaattcgtg ttggggataa aatggcaggt cgtcacggga ataaaggtat tgtttcgcgg
     7321 attgtacgcg aagaagacat gcctttcctc gaagatggaa cgcctgttga tattgtgctc
     7381 aacccgttgg gtgtaccttc gcgtatgaac ttgggtcaga tttttgagag tgttttggga
     7441 tgggcaggta aagaattggg agttcgtttt gcaacaccaa tttttgatgg tgccacgctc
     7501 aacgacttaa atgcttggac agacaaagca gggttgcccc gtgcaggtaa aacttatttg
     7561 tacgatggcg gaaccggcga acgcttcgac caacctgcca cagtaggcgt tatttacatg
     7621 atgaaactgg gacacatggt ggaagataag atgcacgctc gttctattgg accttattct
     7681 ctgattacgc agcaaccttt gggaggaaaa gcccagttcg gcggacagcg atttggagaa
     7741 atggaagtat gggcactcga agcattcggg gctgccaata tcctgcgtga aattcttacc
     7801 gttaagtcgg atgatgtcaa tggtcgtgcc cggacttatg aagcaatcgt gaagggagat
     7861 ccaatgccta ctcccggaat cccggaatcg ttgaatgtat tacttcacga attacgcggt
     7921 ctgtgcttga gcattaattt ggaataggga acgtgaattt atcataacgt tcattcctat
     7981 ccaaaattaa tttaaaattg tcaatttaat taaattgaaa taaaaaatat ggcttttaaa
     8041 actgataata aaataaagag caatttctcg aaaatttcta tcggattagc ttcacccgaa
     8101 gaaattttga aattgtctca tggtgaagta ctcaaacccg aaacaatcaa ctacagaact
     8161 tacaagcctg aaagagacgg tttgttttgc gaacgcattt ttggccctgt aaaggactat
     8221 gaatgtcatt gcggaaaata caagcgtatt cgatacaagg gaattgtttg tgatcgttgc
     8281 ggggtagaag ttacagagaa aaaagttcgt cgtgaaagaa tgggacatat ccaactggtt
     8341 gttcctgttg cccatatatg gtatttccgc actttaccga gcaaaattgg ctatctgctg
     8401 ggaatgtcga gcaaaaaact ggaatcgatt atttattacg aaaaatatgt gattattcag
     8461 ggaggcattc ttgaaaatgg agacaatatt gccaccggag aactactcac cgaagaggaa
     8521 tatttggata ttttggattc gctgccaaaa gataatcaac aactggaaga caccgatccc
     8581 aataagttta ttgctaaaat gggtgcagaa gccatttacg atctgttgag cagaatagat
     8641 ttggatgcgc tttcctatga attgcgtcat aaggccaaca ccgatacttc tcagcagcgt
     8701 aaagccgaag ctttgaaacg tttgcaggtg gtggaagctt tcagagcttc taaaaataga
     8761 aatcgtcccg agtggatgat tgtgaagatt gttccggtta ttcctcctga attgcgtccg
     8821 ttggttccgt tggatggcgg tcgttttgcc acttcagact taaatgatct ttatcgtcga
     8881 gtaattattc gcaataatcg cctgaaacgt ctgattgaaa tcaaggcacc ggaagtaatt
     8941 ttacgcaatg aaaaacgtat gttgcaggaa gctgtggatt cgttgttcga caattcgcgt
     9001 aaagcaagtg ccgtgaagac cgaagccaac cgtcctttaa aatcactttc cgattcattg
     9061 aaagggaaac agggacgttt tcgtcagaac ttgctgggaa aacgtgttga ctattcggct
     9121 cgttcagtta ttgtggtagg cccggaattg aaaatgcatg aatgtggatt gcccaaagat
     9181 atggctgccg agctttacaa accgtttatt atccgaaaac ttattgaaag gggaatagta
     9241 aaaactgtaa aatcggcaaa aaaaattatt gatcgcaagg accctgttat ctgggatatt
     9301 ctggaatatg taatgaaagg acatccggtt ttacttaacc gagctccaac attgcaccgg
     9361 ctgggaattc aggcattcca acccaaactt atcgaaggga tggccattca attgcatcct
     9421 ttagcttgta cggctttcaa tgccgacttt gatggagacc aaatggccgt gcatttaccg
     9481 ttgagcaatg aggctgtttt ggaagctcaa ttgttgatgc tggcttctca caatattttg
     9541 aatccggcca acggagctcc tattactgtt ccttcacagg atatggtatt gggattgtat
     9601 tatattacaa aacctcgacc aaatgctttg ggtgaaggtt tgatatttta ttctcccgaa
     9661 gaagcagaaa ttgcttataa tgaacataaa gtagatattc atgctcgcgt aaaagttcgt
     9721 accaaagatt tggacgaaaa cgggaattcg gtggaaaaac tgattgaaac cactgttgga
     9781 cgaattattt tcaataaata tgttccggaa gaagttggct atatcaacga attgctttca
     9841 aaaaaatcct tgagagacat tatcggcagg gttatagaaa cttgtggaat ggcgcgtacg
     9901 gctcattttt tggacgatat caaggaattg ggctacaata tggcctttag aggcggactt
     9961 tccttcaatt tggatgatgt gattattcca aaagataagg aaaaacttat tgaagaaggc
    10021 aacagggaag tagaagaaat tctgaataac tacaatatgg gattcataac ctataacgaa
    10081 cgttataatc agattatcga cacttggacg cacgtaaatt ccaaattgtc cgatgtgctg
    10141 atgaaggaat tgtcgagcga taatgatgga ttcaattctg tgtatatgat gcttgattcg
    10201 ggagcgcgcg gttcaaagga acagattcgt cagttgtcgg gaatgcgtgg tttgatggca
    10261 aaacctcaga aatcggtatc ggaaggtgga caaattattg agaacccgat tctttccaac
    10321 tttaaggaag gactttcggt gttggaatac tttatctcca cgcatggtgc ccgtaagggt
    10381 ttggccgata cggcacttaa aacggctgat gcagggtatc tgactcgacg tctggtcgat
    10441 gttgctcaag atgtaaccat tactgaggaa gattgcggaa cattgagggg cttgattgct
    10501 accgaaatga aaaataatga agaagtaatt tcttctttgt atgaaagaat tttgggacgt
    10561 gtttccgttc acgatattta tcatccgctt accggagaat taatcgtatc ttcgggagaa
    10621 gaaattacag aatcactggc caaaaaaatt caggattctc ctatcgaaca agttgaaatt
    10681 cgttccgtat tgacctgcga atccaaaaaa ggagtctgtg caaaatgtta cggacgaaat
    10741 ttggcaacag gaagaatggt aaacattgga gaagctgtag gtgtcattgc tgcccagtca
    10801 attggagagc cgggaactca gctgacactt cgtaccttcc atgttggagg tattgcttca
    10861 aatatagctg ccgaatcaaa aattgtactt cgttatgatg gacgtctcga atttgacgaa
    10921 cttcgtaccg ttcagccctc tgatgatcaa agctatcgcg ttgtagtaag tcgtttggcc
    10981 gaaatgcgcg ttgtggatcc taatacgggt attgtgctta ctaccatgaa tatcccttat
    11041 ggatcgaagt tgtatgtgaa ggaaggagat atttgtacta aaggtcagct tatttgcgaa
    11101 tgggatcctt ttaatgcggt tataattgca gaaaacgaag gaaaaatcga atttgaagat
    11161 gtggtcgaaa acgttacttt taaggttgaa accgatgaag ctacgggact tcgagagaaa
    11221 attatcatcg aatcgaaaga ccgaaacaaa gttcctacag ctcatattct ggataaggat
    11281 ggcaatatct tgaaaactta taatttaccg gttggagctc acattgtgtt tgaaaacggt
    11341 gattatataa aaaccggtca gattctggta aaaattcctc gtgctgttgg gaaagccgga
    11401 gatattaccg gtggtcttcc tcgagttacg gaattgtttg aggctcgaaa tccttctaat
    11461 cctgccattg tggctgaaat tgatggagaa gttactttcg gaaaaatcaa acgtggaaat
    11521 cgtgagttaa gcatcacttc caaaaccggt gaagttaaaa aatatctgat tcctctttca
    11581 aaacaaattc tggtacagga aaacgatttt gttcgtgcag gtacgcctct ctctgatggc
    11641 tcgataacgc caaccgatat tttgcagatt aagggaccta ctgccgttca ggattacatt
    11701 gtcaacgaag ttcaggatgt ttaccgcctg caaggggtga aaattaacga caaacatttt
    11761 gaaattattg ttcgtcagat gatgcgcaag gttgaaatca ttgaaccggg agatacccgt
    11821 ttcctcgaaa aacaggtagt tgacaaaaat gattttatgg aagaaaacga cagaatttgg
    11881 ggaaagaaag ttattgtgga tgcgggagat tctcctactt tgaaacccgg ccaaattatt
    11941 acgcccagaa agttgaggga tgaaaacagt atgttgaaac ggaaagactt gcgtccggtt
    12001 caggctcgtg atgccattcc ggcaacatcc aatcagattt tgcagggaat tactcgtgct
    12061 gcacttcaaa ccaacagctt tatgtcggct gcttctttcc aagaaacaac aaaagtgctg
    12121 aacgaagctg ccattaatgc caaagtggac aagctggaag gcttgaaaga aaacgttatt
    12181 tgcggtcatt taatccctgc aggtaccgga cagcgcgact ttgaaaaaat tgttgtctat
    12241 tcccgtgaag aatacgaaaa gaaagttttt gcacgcaaaa gcttaattga tgttgaagaa
    12301 ggcgaagtag tagaagaaaa caattaaaac ttatatctat tttttcaaaa ggcagggctt
    12361 tttttgctct gtcttttttt tattaatgcc cttattttag gaataatcgt aattttatta
    12421 gaaagttttt tcggacaagg cataaacttt ctctttgacc cattgcgccc atttgccgta
    12481 agtttttgaa cggtcaaaag tttgccaacg aataggctcg ccgataacca gcgaaaaact
    12541 tttccctttt tgtttgaaca tttcgtcgga cagatagagc atttcgatat tgaactttat
    12601 atgaaaaaat tttctcagat tggccaggtt gtaaaagaaa tttgaatttt ttccgtcaaa
    12661 ataaatcggg acaatatccc gctgatgttg aatagctttg acaacaaaat tcttgttcca
    12721 tgcttcatcc tgaatttttc cgttggattt tctgctgcat tttccggccg gataagtaat
    12781 cagatgacaa tcggaactga aaaaatcgtt caattcgcga acatggtttt tcccctgtga
    12841 accgactttg ttgaccggga taaacaagtc cttcaagggc tcgataaaca tcaacatatc
    12901 gttggccata aatcgaattt tcccatcata ttccctgccc aacaagctac cggtcattat
    12961 ggcatccatt cctcccaacg gatgatttcc ggcaaaaata taaagtttat cgtcatgagg
    13021 caaattttct ctgtttttaa tggaaacatt gacctgaaaa tattttactg cgctttcgag
    13081 aaaatctaca ttcttttggt gcgaataagt ggtcagcaac tcattgattt ccttttcgtg
    13141 aacaatttta cgcaaataac tcaccaaaaa agaagggata aaagtattag gcgctttttc
    13201 cttgacaact ttttctatat caattaaaaa atccggttta ctattcatga aaataaaatg
    13261 gtgttgttct attgcaaagt tacaataatt tgaaattttt gtgtttttta tcattgcatt
    13321 tttcgttgaa aagttatcaa catttttgaa attcacaaat atttttcacc acttaccata
    13381 taaaaatgaa tgaataaaat ataaaatctt tattttctga tgtttacaaa ataattgtct
    13441 aatttcttgt tgttgataaa ttgttgaaaa gcggttgaaa acaagctaaa ataatttaaa
    13501 aaaattgttt ataacttttt tgtgggtgaa tgtggggaat tgaaaataat tccatatttt
    13561 tacagttgaa aaaatatagc tactgcaatg tctacattta ttggaaaata tgaagccaaa
    13621 gccgacgtaa aaggtcgcat attcattcct tccgcttatc gaaaaatctt gggcgtagac
    13681 gaccgtgaac aaatcatcat gcgcaaagac gcagggaatg aatgtattgt gttctatccg
    13741 gaaagtatct ggaatcaaaa ggtagaaaac ttaaaatcga aattggacga atggaatcct
    13801 tccgatcagc ttttactgat gcagtttgtg gcagatgcgg aatggctgga tattgatgcg
    13861 cagggacgtg tattgatttc gaaaaaacat ttgcagacca ttggcgttga aaacgcagaa
    13921 gtgctttttg tgggcatgat tgaccgtttt gcgctttgga gcaaaagtcg gtacgaacaa
    13981 atgcaattgt cagccgaaaa ttttgcggct ttgctgaagg aaaaaatgtc aaaataacta
    14041 attataattt catgtctgaa aaggaatatc acatacccgt catgctgaag gaaacgctgc
    14101 aaggattggt actcaatccg gacggtatct atgtggatgt aaccttcggc ggcggaggac
    14161 attcacgtgg tattctcgat ttactgagca aaaagggaaa acttatcgct tttgatcagg
    14221 accaggacgc ctgccaaaat atttttgagg atgacagatt tttgttcgtt caaagtaatt
    14281 tttgttattt aaaaaacttt ctacgttatc atggtatttc tctggtagat ggcattttag
    14341 ccgatttggg cgtttcttcg catcactttg atgagagcga acgggggttt tcgttccggc
    14401 ttgatggaga attggatatg cgcatgaaca gaaaaacgga actgacggct gctaccgtgc
    14461 tgaacaccta cactgaagaa cagttggccg atgtttttta tcattacgga gaattgtcca
    14521 atgcacgaaa aattgcagca accatagtaa aagcgagaga cagggaacct tttcaaaaaa
    14581 ttcttccgtt tgtagaaatg atgaaacgct ttttcccccg tcagaaagaa aaaaaaggac
    14641 ttgcctgcgt ttttcaggct ttgcgcattg aagtaaatca cgaattggaa gctttgcagg
    14701 aatttctgca gcaaaccccc gatgtgctga aacccggagg aagattggcc gtgctcactt
    14761 atcattcgct cgaagaccgc atggtgaaaa attttatgaa aacagggaac tttgaaggta
    14821 ttttaaacaa agattttttt gggaatatca ttgctccctt caagcctata agcggcaagg
    14881 ctattacgcc acagctttcg gaaattgaaa aaaatccgag agccagaagc gccaaattac
    14941 gcattgccga attgattgac aaaactaatt gattgatttt ttgaacattt aatgaaagag
    15001 ttaaactttg aaataacgaa tgagttggtt cagtaaaatc atagagaaca taaagggcag
    15061 tgaggatttt tccgaactca agtcgagctc atttcgggat atcctcaacg gaaatatttt
    15121 caccaaaaga ttttttatca agcaaatagg tcttttgatt atgattgtgg tgctttttat
    15181 attttatatt ggcaaccgct acgaatacga atcggaattg acctaccaaa acaaattgaa
    15241 gaaggaagtc caggacaaaa aatatgaatc gcttagcatc tcggcagaac tgatgcaagt
    15301 cagccgtcag tcgaacgtgg aaaaaatgtt gaaagaaaga ggcatcgaac tgcacgagcc
    15361 ggtttctcct ttggtagtgt tggacgattc gatacaataa gaattgatat ggcagaccaa
    15421 cataaaaata ttgtatttcg ctttggcatt gtatatgttt ttatagtcgt tgcctttctg
    15481 ctggtgattt acaaaataat taccatacag ttttttgagc gcgaaaattg gctggctttg
    15541 gcagcaaaaa atgtaaaaac caatatagaa gtgaaagcca acagaggaaa tattttttct
    15601 tccgacggac gtttgatggc cagttccata cctacttatt acatttacat ggacttacgg
    15661 gtgccggcat tgcaagaaaa caacggacaa ctgtttcacg aaaatattga ctccttgtcg
    15721 ctttgtttgt caaaattttt taaagataag tctcaagccg aatacaaaaa agacattacg
    15781 gaagcttaca acaaaaagtt gggagctttt cgcatttatc cgaaaaaaat ctcttatagt
    15841 caattaaagg aattaaaaac ttttccgttg tttcgtttgg gaaaaaacaa aagcggtttg
    15901 ataacaaaag aacaatttag aagagtaaaa cctttcgggt cgctggcctc ccgaaccatt
    15961 ggagatattt atgctgacga aaccaaaggc ggaaaaaacg gtcttgaact taaattcgac
    16021 tcccttttaa gaggaacacc cggaatagcc attcgccaga aagttgccaa tacatggcag
    16081 gaaacggtac aagtggaacc cattgacggg attgatgtga ttaccactat tgatgttgac
    16141 attcaggata ttgccgaaaa agcgctgttg gacagtttaa ggtctttcga tgcgcaggaa
    16201 ggttacgtta ttgtaatgga agttcaatcg ggagaagtta aagccattgt aaacatgcag
    16261 aaaaatgaaa atggaaccta ttccgaaaat cgaaacggcg ctgtttccga tatggtggag
    16321 ccgggctcta cgtttaaagt cgtttctctg atggctgcac tcgacgacgg taaagttaaa
    16381 gttaccgatt caatagatac cggaaacgga atttttcagt ttgctaatgc ttttatgaaa
    16441 gatcacaatt acaacagagg cggttttcat aaaatcacca tagccgaagc aattcatgct
    16501 tcttctaaca tcggaatttc ccgaatgatt gtaaatgctt acggcaacaa tccggctgcg
    16561 tatgtagata aactgtacaa aatgaaagtg aatgaaccaa tgaatttgga aattcccgga
    16621 acagccgctc caaatatccg tcatccgctg gataaatcaa aatattggtc gaaaaccact
    16681 ttaccttgga tgtcgatagg atatgaaacc caaattccac ccatatatac tttggctttt
    16741 tacaattcaa ttgccaatga tggaaaactg atacggcctt attttgtaaa agcgctgagt
    16801 aaaaacggac aaattttaaa aacctttcag accgaaacca tcaatgaaca aatttgcaag
    16861 ccatctgttt tgcaaaccat tcgagaaacg ttgttggggg ttgtagaagg ccgattggga
    16921 acggctaaaa acgtacgttc caaatacgtt cgcattgcag gaaaaacggg aacggcacaa
    16981 atttcaaaag gtgcgctcgg ttatcgttcg ggaggaaaat cacaccaagt gtctttctgc
    17041 ggatattttc cggccgaaca tccactctat acttgcattg tcgtgattcg tgaaccgaga
    17101 atcggctatc cttcgggcgg caaaatgagt ggttcggtat tcaaagatat cgccgaacga
    17161 atcatgtttt tgagatccac attaaaaccg gaacatttcg taccggatac catgcgaaat
    17221 gtaccaaaag ttccttatgc tttatggggc agtttcagcg atttggaaaa agttacgtca
    17281 gaaatgcgac tgcctatttc gggcaaggca ggaagttttg tcaacgtgtt gcccgatgaa
    17341 aaaaataatg gtttggaaat ttcaaaaaaa accgtttaca ggcaattgat gcccgattta
    17401 aaaggaatgg gagcgaaaga tgctgttttt ctggctgaaa aattaggctt aaaagtaatg
    17461 ataagtggtc gcggaaaatt ggtttcgcaa agtatcagcc ctggcacaaa aataacgaag
    17521 ggtcaaatcc tttctcttca ttttcagtaa attaaaaaca aaaagttatg attttgaaag
    17581 tcgctttaga gaaaattttc ccaactgaaa ttattggaaa tacggagatg gaaatttccg
    17641 gtgttcagtc cgattccaga aaagtggata aagggaatat ttttgttgct actcgtggca
    17701 ccaatgttga tggacacaat tacatttctc cagccattga gaaaggagcc tctgccatag
    17761 tatgcgaaac gtttcctgaa actatccagc agggaatcgt ctatattcgc gttgaaaaca
    17821 gtttggaggc attgggactt ttggcttgtg catggtatgg caatccttca gaaaagctga
    17881 cattggtcgg cgttaccggc accaacggaa aaaccaccat tgctaccttg ctttaccaaa
    17941 tgttccgtca gctcggatac aaagccggac tgctgtctac tgttcgaaat tatgttgtgg
    18001 atgaacctgt agaagcaacc cataccactc ccgatccgtt ggagttaaat catctgctcg
    18061 ctcaaatggt tgaagctggt tgtcaatatg cctttatgga agtaagttct cacgccatcg
    18121 atcagcgtag aattgccgga ttgcttttta aaggaggaat tttcaccaat attacgcgtg
    18181 atcatctgga ttaccatttg acttttgaaa attacctgaa agcaaagaaa aaattttttg
    18241 acgatttgcc cgaaaatgct tttgctttaa ccaacttaga tgataagaac gggctggtca
    18301 tgctgcaaaa cacaaaagca aaaaaatata cttattctct cagatcgatg gccgatttta
    18361 aaacccgtat tctggaacag gattttgaag gaatgctttt ggaaatgaac ggcaaacaag
    18421 tacacgtttc atttgtagga cggttcaatg caagcaatct gaccgccgta ttcggagcag
    18481 cggtactgat gggagaagaa gaaacagaag tactgagaat tctgagtttg cttcatcctg
    18541 tttcagggcg attggaaacg ttgcatgctc cgtctggata taccgttatt gttgattacg
    18601 cacatactcc agatgctttg aataatgtga tcagcaccat ccatcaggtt ttgcaaaaca
    18661 aaggccgact aattacggta gttggctgtg gaggaaatcg agacaaggga aaacgtccct
    18721 tgatggcaaa agaagccgta accggcagtt ggaaaaccat tctgacttcc gacaatccac
    18781 gttttgaaga tccgatggac atactgaacg atatgattgc cggattgacc gacgaagaaa
    18841 gaaaaacaag ccttgtgatt cctgaccgcc gtcaggccat tcataccgcc tgctcgctgg
    18901 ctcaacccgg cgatgtggtg ctgattgcag gaaaaggaca cgaagactac cagattatta
    18961 agggcgaaaa acatcatttt gatgatcgag aagtggtaaa agaatgtttt taaaagattt
    19021 tattcggcta taaaagaaaa aaaattcaaa ttactaacca agactttcct gttttcagaa
    19081 aaggcgaagt caaaaaaaga aacggacatg ttctatcatt tatttcgcta tttggacaag
    19141 ttttacgata ttcccggagc aggaatgttc aattacattt cgttcaaaac gggtttgtcc
    19201 tttatttttg ccttgttgct ttccacgctt tttggacgga gaatcattga ttttttgcaa
    19261 aagaagcaaa ttggcgaaac aatccgcgat ttgggattgg aagggcagct ttcaaaaaaa
    19321 ggaactccca ctatgggtgg aattattatt atcaatgcta ttcttatccc gactttactt
    19381 tttgccgacc tgaccaatat ttatatcatt ctgatgctca tcaccactgt ttggatggga
    19441 gcaattggat ttgttgacga ttacataaaa gtatttctga aaaacaagga aggacttagc
    19501 ggcaaagcga agatagttgg tcaggtagga ttaggactga tagttggatt ggtaatgtat
    19561 ttcagtcctg acgtggttat gcgcgaaaac accgaaatca gaggagaatt gcgagttgaa
    19621 gatgtagtat attcaaaaaa ggatatcaaa gctacccgaa ccaccattcc ttttgtgaaa
    19681 aacaatgacc tcgattattc aaatgcgttt aagaatgcgg ggaaaaatgc acaaaagtat
    19741 ggatggattc tgtttgtact ggttgctatt ttcattgtaa cggcagtatc aaatggtgca
    19801 aatttgactg atggattgga tggactttca gccggttcgt cggcaattat gggaggtgca
    19861 cttggcgtac ttgcctactt gtcgggcaat atcaactatt ccggttacct aaatattatg
    19921 tatattcccg gaaccggaga actgctggtc tttgccggtg cttttttggg tgccaccatt
    19981 ggttttctat ggtacaattc tttccctgca caaattttta tgggcgacac ggggagtttg
    20041 acgttgggag gtatcattgc tgtttttgcc attgctatca gaaaagagtt gttgttaccc
    20101 attttgtgcg gtgttttttt ggccgaaagc ctgtcggtaa tctgccaggt gagctatttt
    20161 aaatacacaa aaaaaaggta cggtgttggc aaaagaattt ttaaaatgac acctttgcat
    20221 catcattttc aaaaacccgg aaatgcggga attgaagcgt taattcaacg acccttgcaa
    20281 gcagttcccg aaccaaaaat tgtagtcaga ttctggattg tgagcatgat tttggctgca
    20341 ttaactatta ttacattaaa aatcagataa attcttggaa aatttcagaa aattttgttc
    20401 aaaaaagata tgaaacgaat cgttgtactt ggagcgggag aaagtggagc aggcgctgcc
    20461 gtattggcaa aacagcaagg ttttgatgtg tttgtttccg acttgtccga aataaaggaa
    20521 aactacaaag agttgctcaa ccagtatcaa atagaatggg aagagaaaca acataccgaa
    20581 gcgctgattt tgaatgccga tgaagtaata aaaagtccag gaatccccga taaagcgcca
    20641 attatcaaaa atttgaaaga gaagaatatt cccattattt ccgaaattga atttgcaggg
    20701 cggtttacaa atgccaaaat gatttgtatt accggcagta atgggaaaac aaccacaacc
    20761 atgctgactt atcatatttt gaaaaatgca ggttacaatg ttggtctggc aggcaacgta
    20821 ggacaaagtc ttgcctatca ggtagcaaca agttcgcgcg attactatgt ggtagaaatc
    20881 agcagttttc agttggacgg gatgtatgat tttaaagcag atgtagcaat attgctcaac
    20941 ataacacccg accatttgga ccgttatgaa tataattttc aaaattatgt cgattcaaaa
    21001 ttccgtatca tccgtaacca aacctctgaa gacgctttca ttttttggga cgatgatccg
    21061 gttattcagt cagaaatagc aaaacgctct atactcgcag caaaatatcc cttttcactc
    21121 aaaaagaaag agcattgtaa agcttatatc gaaaacaaca acctgatcat agaaacttct
    21181 aacaacccat tgattatgcc ggtaaaagaa ttgtcgttaa aaggaataca caacacctac
    21241 aatatgatgg ctgccggttt ggcggcatcg gctgtgaatg taaaaaaaga atttattcgc
    21301 cagtcgttac aggattttca aggcgtggaa catcgcctcg aatatgtagc tacagttaaa
    21361 aacgtaactt tcatcaacga ctcgaaagcg accaatgtca attcatgctg gtatgcgctg
    21421 gaaagcat
//
LOCUS       DAOTAX010000032        21014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2057_length_21014_cov_29.746887, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000032 DAOTAX010000000
VERSION     DAOTAX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_21014_cov_29.746887"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLM04_04940"
     CDS             <1..702
                     /locus_tag="PLM04_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02453.1"
                     /translation="FCVTGQDSSPQSLTGHIVKRCGQFKKTLMKHLKILTIIVLTLIW
                     TCGFGQTDIDNEIISALINSEFKSLPNDTIFNRKGEIKRIKTYKKTDILLIIETETFL
                     FDPEVDSFDRFKNETNGLPSLDSLTYLDFIDKNKTKIQIDSIYGFQGTITYNSRQEID
                     NIFKQGGWDNYHKIHGYKSMIKVSRPGLNKNMTKAFIYYSSSSGGLSGAGFYLILENI
                     DGKWIVKESMLAWIS"
     gene            complement(1426..2871)
                     /gene="amrB"
                     /locus_tag="PLM04_04945"
     CDS             complement(1426..2871)
                     /gene="amrB"
                     /locus_tag="PLM04_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HON02454.1"
                     /translation="MEKFNETIDRRPAVAGKFYPANVEELQKEIDIFFKNAAPKQLDR
                     VRAIICPHAGYVFSGKITASAFNQIDANREYKRIFLIGSSHHKYFDKASVYCSGNFLM
                     PYGEEKVDMDFGRKLVENFPEWFTDDRTPHLNEHCLEVQLPFLHHVLRKNYVIVPILI
                     GTAHPSVCKQIASVLKPYFNEENLFVVSSDFSHYPAYQDAKYVDSLTKDAICSNRPEK
                     LLETLAENERKHISNLQTSLCGWTSVLTLLYMTSSNKSLKFHAVDYCNSGDVKYYGER
                     DRVVGYWAIAVAESKHAAEDFYLTEKDKKDLLKISRKTLEEHCRAGRVSEVDTATLSE
                     TVKKNCGAFVTLHKRGALRGCIGILTAEKPLYKTVQEMTIAAASHDPRFMPVEASELP
                     FIDIEISALSPLKKINDISEIELGKHGILIEKNFHRGVFLPQVATETGWSKEEFLGHC
                     SADKAGLGWDGWKTADIYIFTATVFSEKDFW"
     gene            3464..4315
                     /locus_tag="PLM04_04950"
     CDS             3464..4315
                     /locus_tag="PLM04_04950"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HON02455.1"
                     /translation="MDKKYNALSFSTSKFVTKFYSTSFYSASQYFSKEIQEAIFSIYG
                     FVRLADEIVDSFHFSDKEYLLQKFESDYYDAVKNGISLNPILHSFQMTVGKYGISDEY
                     VQHFLSSMKADLEKKEYKSQIELNTYVYGSADVVGLMCLKVFCKGDEQLFDRLMLPAT
                     KLGSAFQKVNFLRDIKQDTQELGRHYFPQIIEEGLTEQTKKELVEDIRKDFDDAYKGI
                     VLLPADSRLGVLIAYKYYTALLRKIEKTAACDILNKRIRISDAGKMFLVLSTQIKYYL
                     RAFYGNK"
     gene            4302..4856
                     /gene="idi"
                     /locus_tag="PLM04_04955"
     CDS             4302..4856
                     /gene="idi"
                     /locus_tag="PLM04_04955"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HON02456.1"
                     /translation="METNKENFVQLVDENDQPVGIMEKYEAHKKAALHRAISVFIVNS
                     KGDWLIHRRALDKYHSNGLWTNATCSHPYPDESYLEAAKRRLKYEMGINCELIPLFHF
                     IYQEKVNDELTEHELDHVFFGVSDDIPIHNTGEVEEWKYIPYRQLQEDVENHPEKYTV
                     WFKIILKKVKAYIDTENLTESSCR"
     gene            4847..5512
                     /locus_tag="PLM04_04960"
     CDS             4847..5512
                     /locus_tag="PLM04_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HON02457.1"
                     /translation="MQISLLLQKFKQKYPERKVRQFIFTFYAVGIVGLALPATHVFFV
                     YLIPLALLLSAFILLPFQEDDFSIKTGVLFTVIFVVSFTVEAIGVNTGKIFGQYFYEN
                     GLGVKCFGTPLIIGLNWALLVYLTASIFQSFRINKFFLVLFPSVLMVLYDVVLEQVAP
                     KMQMWFWEADRVPFRNYLVWFLMALIFHTLLVISGLKLKNPLSKIVFSAQFLFFLILM
                     FIL"
     gene            5522..6148
                     /locus_tag="PLM04_04965"
     CDS             5522..6148
                     /locus_tag="PLM04_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HON02458.1"
                     /translation="MLKAKHHCLIYPFFRRYTLLLMKRNFHSVNVIRTNFSRNENLPV
                     LMIANHISWWDGFWAMYLNLKVFHKKFYFMMLEDQLLKFRFFQWTGGFSIKKKSREMI
                     ESLQYAAKILDNPDNLLLIFPEGKLQSLYNPLHVFEKGIERIVEGHKNNFQLVFVANF
                     IDYLSHKKPDLYMYVSEYQGNVFSHSKLQEAYNVFYGNCLDIQKSKTE"
     gene            6152..7243
                     /locus_tag="PLM04_04970"
     CDS             6152..7243
                     /locus_tag="PLM04_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON02459.1"
                     /translation="MIYLAWVVFGFTFFQLCIAGLNYFFREKIPISTNRISDEKISVL
                     IPARNEEQHIGNLLNDLSGEDDNILEIIVFDDLSSDHTVEIVNQFIGTNEKIRLIRST
                     HLPAGWLGKNFACYSLANAAKGNYFLFLDADVRMEKEIVVSSVKFFKQKSLHLLSIFP
                     TQLMMTKGEKMTVPNMNYILLTLLLLPLVRKLSFPSISAANGQFMLFDADTYKKFQPH
                     EKFKDKRVEDIEIARFYKKLKLKIACLTGIKQVRCRMYENFAEAVNGFSKNVIHFFGN
                     SFLIAVLFWIVTTFGWLAVWLAFGLPTLAVYLLAVVCIRIFFSLASNQSILDNLIFFI
                     PQQFSLGIFIYQALINRSKNQYLWKGRKV"
     gene            7222..7971
                     /locus_tag="PLM04_04975"
     CDS             7222..7971
                     /locus_tag="PLM04_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02460.1"
                     /translation="MERKKSLVLFFLLILLHLHVHASHRENIYKAYINNDMQAWKNTI
                     DKMNLSSGNASDEFLLELVNYEYGYIGWCIGNKKTAEAKEYLKKAENRLITLEKNQFR
                     LSMIEAYKSAFYGFQIGFNKLKAPVLGPKSVEAAKLAIQYDPNNYFGYLQMGNIYYYS
                     PAAFGGSKAKAIESYRQAEKLILSNNHRLKNDWNYLSLLVTLAQAYENTGNDESAKIY
                     YQKILNVEPDFAWVKNKLYPEFLKKTQKNNI"
     gene            7968..>8173
                     /locus_tag="PLM04_04980"
     CDS             7968..>8173
                     /locus_tag="PLM04_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HON02461.1"
                     /translation="MKEKVIIVGAGLGGLATGLRLAKEGYEVEFLEKNTKAGGRLNQI
                     KKDGFTFDTGPSFFSMSYIFKEFA"
     assembly_gap    8174..8273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8274..9430
                     /gene="crtI"
                     /locus_tag="PLM04_04985"
     CDS             <8274..9430
                     /gene="crtI"
                     /locus_tag="PLM04_04985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HON02462.1"
                     /translation="LAKQFDDVEPDFELKMEKYLQKSAQLFNDTVDIVIKRNFDSYLD
                     YFLALMKVNPVHLPILFKTFWQHVCDYFTSKQAREIISLVAFFLGRTPFDTMGIYSLL
                     SHVEFRHDGYYNVKGGMYKIVEGMLEELSKLDVKFHYQTEIVAYHAENRKIVSLKDAQ
                     GNLWKADKFVINADAALFRSKVMKYKNFSEKKLDKMEWTMGYLTFYLGLKCKLPQVNH
                     HNYFLGSNYEQYAGNILQNPDTLEKPYYYVNVVSKYNPECAPEGCESLFFVCPVPNLK
                     YKNDWSDREKIVNSIIDDFSQRIKKDISAEIISKTIFTPQEWRDKFSLYQGSGLGLSH
                     KFTQIGAFRPKNYDEKHDNLFYVGASTIPGAGLPMAVISAELVVERILGK"
     gene            complement(9554..10498)
                     /locus_tag="PLM04_04990"
     CDS             complement(9554..10498)
                     /locus_tag="PLM04_04990"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HON02463.1"
                     /translation="MAASNSSFKVFSGTNSRSLAEKICQSLGCPLGKMNIEHFADGEF
                     AVSYEESIRGLNVYLVQSTFPNSDNLMELLLMIDAAKRASAYKVIAVIPYFGWARQDR
                     KDKPRVSLGAKLIADMLSVAGIDRLITMDLHADQIQGFFNVPVDHLYASTIFAPYIQT
                     LRLKNLVIAAPDVGASKRAGSYSKYFDVPMVLCHKTREKANVVGEMRIIGDVKGKDVV
                     IVDDMVDTAGTLCMAANLMMEQGANTVRAIVSHGVMSGSASEKVEKSALTELAFTDSI
                     PFDASKCSKVKILSIADMFADTICRVQEHKSISEQYIF"
     gene            10792..13485
                     /locus_tag="PLM04_04995"
     CDS             10792..13485
                     /locus_tag="PLM04_04995"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HON02464.1"
                     /translation="MKIHFKIPYYTHWGQQIFVCGNLPELGNNVPEKSFQLHFQNTED
                     WYGEIEIHQSEHFELEYRYLLYEENTKRFTYEWIEDRKITIDPRMEHIFCFDFWNAPG
                     AVENVFFTAPFKKVFFSDNFNHSQKTRSKKNFTHIFRIKAPLLKNHQVPCLIGDCKVL
                     GSWNTVSPLLLTRDGENWWKAEVDLGKEKSDVHYKYGIYDTENGQFLMFENGFDRTAP
                     VFNAKNTFVRLSDGFLRMNSQSWKGAGVSVPVFSLRTKESFGVGDFGDIRLLVDWAEK
                     AGLKLIQVLPLNDTIGTHTDADILPYAAISAFALNPLYLNLPSLGKLPENHPLQKEYE
                     ERQPQLNALEWIDFLEVVNFKLKYVKALFSFHQEEFLQNKDFLTFFENNKHWLIPYAA
                     FCVLRDRYGTPDYHQWKEFSTFNAEKIAAFVSANQPHYADIALNYFIQYHLHIQLMEA
                     ASYAHHHGIVLKGDIPIGVNKNSVETWAHPELFHIDMQAGAPPDMFSIKGQNWELPTY
                     NWEAIKQTGFDWWKKRLKQMSNYFDAFRIDHILGFFRIWEIPNDQLEGIMGHLYPSIP
                     VYLDEFKAKGIWFDYNRFCKPFINDQVLEQLFGTDAGWVKENCLQIEDGWILRLKPFY
                     RSQKNVQQLYEEGQFSENIRNGLFDLISNVLFFEVEGSHGTRFYPRWGMHSLSTFQYL
                     DDFTKQKLDEIYIDYFYHRQDEFWFQSGMEKLPVLKQTTDMLICGEDLGMMTPCVTRA
                     MKELGILSLEIQRAPKTNKIEFFHPSDAPYLSVVTPSTHDMSTIRGWWEEDRNITQRF
                     YNTQLGHWGEAPYFCEWWICRDILLQHVYSPAIWAIFQIQDLLGVSEKLRRENPHEER
                     INVPSDSKKSWRYRLHLNLEDLLKEDDFNRQIRNYIRQAGR"
     gene            complement(13699..16179)
                     /locus_tag="PLM04_05000"
     CDS             complement(13699..16179)
                     /locus_tag="PLM04_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02465.1"
                     /translation="MHSRKLFSFVFFSILSISLFSQNKVRIYGYVIDTNNRGIELANV
                     YVQHTNIGTSTNQNGYYELTVDIKDSIKLVFSMIGYQTVTYTVHPKLKVIQLTVELPP
                     LSKQIEDVNVIGQRRQTSTIDVVDPSKYRLMPNSAGGIESLLISFTGVSSSNELSSQY
                     NVRGGNFDENIVYVNGIEVYRPLLIRAGQQEGLSFINPDMVQNVSFSSGGFNAEYGDK
                     MSSVLDISYKKPSAFEATASASLLGASAYVGTAGKRFSQMHGIRYKTSKYLLGTMDTQ
                     GEYDPSFVDYQTFLTYQIKPKWELTFLGNFSQNDYRFIPQTRETSFGTYSMGRKLTIF
                     FEGQEKDLFQTSFGALTLNFRPSKSTKLSLIGSAFYTNERENYDISGEYILSEVKMDV
                     DPDKQTGESLGIGNYHEHARNKLNATVANLSHLGEYAKNGHLLKWGANVQIEKIDDQI
                     NEWEWRDSAGYSLPYNNRQVNLYYNMNASNSLKTLRTTSFLQDTYKYSSTAGTFSFTG
                     GLRSHYWTFNREFLLSPRFTVAYLPHWEKDFSFRFATGLYYQAPFYKEIRDTLTDALG
                     NVDIKLNSNIKAQRSLHFVLGGDHYFRAWGRPFKFTTEAYLKLADRVVSYSVDNVRIR
                     YSGENDAKAYTAGIDFKLFGELVPGTDSWINLSLMDSKEDIIGDSYVRHTYDDEGNIL
                     TSETVYPSWVPRPTEQRYAFSMMFQDYLPSNPKYKLQLKLIWSDGLPFGAPRNEQFRS
                     AFRMPPYRRVDIGGSRLLVNGSDEWMNKSWLGHVKNIWINVEVFNLLDFKNVNSYYWV
                     TDIYQHQVAVPNYLTGRQLNLRLLVDFK"
     gene            16620..17543
                     /locus_tag="PLM04_05005"
     CDS             16620..17543
                     /locus_tag="PLM04_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HON02466.1"
                     /translation="MKISNLLVILGLLFCFSFVQAKSIQKITRTYAVKDSLELKMDIY
                     TVDSTSSSLQPCLVFVFGGGFKEGKRDEPTYQPYFDYFAKKGFMVVSIDYRLGMKDQR
                     SPGVFNTKPLQNAISLAVLDLYSATKFLIEKADSLKIDKNKIIISGSSAGAITVLQAE
                     YELKNHHAITSELPHDFRFAGVISFAGGIFSTKGMPSYAESPAPTLFFHGSADKLVPY
                     KETRLFNKGMFGSRSLTNKFHKKKYSYLFYSMEGLGHEVASFPMKEFLPEIEQFIQNF
                     VFEQKPWMVEVYFTDKERKTEKSVTPADYYK"
     gene            complement(17605..19332)
                     /locus_tag="PLM04_05010"
     CDS             complement(17605..19332)
                     /locus_tag="PLM04_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02467.1"
                     /translation="MKKHQKKILIALLLFSNILFVVAQDVPEHISYSRIYDFLDELAN
                     EKLIEINSVVKPYSRTFIAQLLQQAKSKEAKLNRRQRKELYFFLENYALEQNRLPQYD
                     YPLFEQTNWQLDVFPPSVHYRDSLLRGRVMPILGMNILYNNNDKITQRWFGIDFQSMI
                     GKHFAVYASLRDVSQQGELLAESTYLTPYQGGAYKRTTNGGDYSEMRGGISYAWEWGS
                     VALTKDHVQWGDNYHGSNIIAGRTPSFPMLKLSLKPAKWLEMNYFHGWLTSNVIDSTR
                     YYLSNQNEKMYRYTNKFLAANMISFIPLPQLHLSAGNAIVYAESNVQPAYLIPLAFYK
                     SMDHTLTMGTENQNSQLFLNISSRNIQHLHLFTSIFVDEFSISRIKPSSKEKNPISYK
                     LGFRLSNFPLENLSLRGEYTKNAILSYKHSIPALTWATNGFNLGNYLGDNSQEIYAAF
                     KYKPMRGLDINLSWTQAEHGNEYAYIRHGTWQGSKASVLDIISQPFMKDKIWSNKTLA
                     LHVKYELFPNTYATLNIENSYIQTFNAVSEVTFGERRMTAEEALNHYTPKFLQGKQTT
                     FTAGFSFGF"
     gene            complement(19514..>21014)
                     /locus_tag="PLM04_05015"
     CDS             complement(19514..>21014)
                     /locus_tag="PLM04_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HON02468.1"
                     /translation="KPKWNSILVDLILLSLNFLIIYGWFPLTTPVPFQKYFIPSLLFT
                     GSWLIFSYFFQRYLPLRKQTYSQALVKLLLVGSLQFVIYVTILHHFFKQYSGFVILAV
                     TIGIFTVEFLYLFFYFSYRFATQFDISISDEKERPQGKPREPREIDEESYFNLINFIE
                     QYSGKKALKYLQSRFDLRKDDVRVFLNTSIENLELIPHYHYSTFIYLRKLNDTEKINT
                     FFSGINEKLPDDGNFVCCFEKKSTRKKKFLQKYPPGINYILYSLDYFFKRFIPKLFFS
                     RRIYHSLKGGKNRILSKAEVLGRFYCHGFEVIEEKKIDGLLYVWAQRKRNPTPVYNRA
                     YGPLIKLKRYGKNGKEIYVYKFRTMHPYSEYLQEYVYQRHSLQDGGKFNKDFRVTTIG
                     KFMRKYFLDELPMLINIFRGEMKLIGIRPLSQQYLNLYNDEIQQLRMKHRPGLLPPFY
                     ADMPRTLDEIQESERKYLLACEKNGVLWTDIKYFFLILKNILFHHARSA"
BASE COUNT         6613 a         3775 c         4033 g         6493 t
ORIGIN      
        1 ttttgtgtaa ccggacagga tagtagcccg caatccctta ctggccatat tgtcaaacgt
       61 tgtggccaat ttaaaaaaac actcatgaaa catttgaaaa tactgacaat aatagttctc
      121 acactgattt ggacatgcgg atttggacaa actgacattg ataatgagat aatctcagct
      181 ttgataaata gtgaatttaa atcattaccc aatgacacaa tttttaatag aaaaggagaa
      241 attaaaagaa ttaagactta caaaaaaaca gatattcttt taatcattga aacggagact
      301 tttttatttg accccgaagt tgattcattt gacaggttta aaaatgaaac aaatggactg
      361 ccttctcttg acagcttgac ttatcttgat tttatagata aaaataaaac aaagatacaa
      421 attgattcaa tttatggttt tcagggaaca attacatata attcaagaca agaaatagat
      481 aatatattca aacaaggtgg gtgggataat tatcataaaa tacacggata taaatctatg
      541 ataaaggttt cacgtccggg attaaataaa aatatgacaa aagccttcat ttattatagt
      601 agttcaagtg gtggattaag tggagctgga ttttatctga ttcttgaaaa tatagatggt
      661 aaatggattg ttaaagaatc tatgcttgca tggatttctt gaccaaatga ctgattgtga
      721 atgataaatt ggccacaaca ctcggtataa ccaataaggg tttcagtggt ttgcgaaggg
      781 ttgtagtctg cttcaacttt tgtgtaaccg aacaggaaat tatccccaat cggcaacttt
      841 tcataccacc gaacgttgtg tgcaagctta aaaaaagcaa taaaaactct attaaatgtc
      901 gaaaaaagaa caaaaatatg aacgaaaaac cttttaagga cgatcaaact ttgaaaaaaa
      961 taattttatg ttaactgctt gccacacgat gcgattattc ggcagaagag ttgtatgaga
     1021 tttttgaact aaacattaaa aaatttgatt attctatctc tgacacagtt attgaaaagc
     1081 tgaaagcatt tttcaacaat tcagtagcgc acaaggataa aaactttggg gatgctcgct
     1141 ttgtcttctt tgaaaaaaca actgttgaaa aagtaaaatt acacacagcg agttcaaaac
     1201 cttatccttc aagcaaatct tagtaaccaa caatatgtcc acgtgaacca aaccttaaag
     1261 ggttgcttcg gattctacat cctcgcgagg agaggactat taaaagtagg aataaaggat
     1321 tgttgaataa tgttttgatc gaaaaattat ttcttgcttt tcaaacaaaa cacaaataaa
     1381 tacttgattt tcagtttacc aaaactttgc caaaattatt ctattttacc aaaaatcttt
     1441 ttcgctgaaa acggtcgccg taaaaatgta aatatcggct gttttccagc catcccaacc
     1501 caaacctgcc ttgtcggccg aacagtgtcc gagaaattct tccttactcc agcctgtttc
     1561 tgtagctact tgaggcagaa atacgcctct gtgaaaattt ttctcgatta aaattccgtg
     1621 tttccccaat tcgatttcgg aaatatcgtt gatttttttc aggggcgaca aagccgagat
     1681 ttcaatatcg ataaaaggca gttcggatgc ttcgaccggc ataaatcgcg gatcatggga
     1741 agcagcggct atcgtcattt cctgaacggt tttgtacaag ggcttttcgg ctgtcaaaat
     1801 accgatacaa cctcttaaag ctcctctttt atgcaatgtt acaaaggcac cgcaattttt
     1861 tttaacggtt tccgacaaag ttgccgtatc cacttccgaa actcttccgg ctcgacaatg
     1921 ttcttcaagc gtttttctcg aaattttcag caggtctttt ttgtcttttt cagtgagata
     1981 aaaatcttcg gcagcatgtt tactttcggc tactgcaata gcccaataac ccacaaccct
     2041 gtcccgttcg ccataatact tcacatcccc cgaattgcaa taatccaccg cgtggaactt
     2101 aagcgatttg ttcgaagaag tcatataaag cagcgtcaat accgaagtcc aaccgcacaa
     2161 actggtctga agattagaaa tatgttttct ttcattttca gccaaagttt ccaacaattt
     2221 ttcgggacga ttgctacaaa tggcatcttt ggtgagggaa tcaacatatt tggcatcctg
     2281 ataggccgga tagtgagaaa aatcggaact gacaacgaat aaattttcct cgttgaaata
     2341 aggttttaat accgacgcta tttgcttgca aacggaagga tgggctgtgc caataaggat
     2401 aggaacgata acataatttt ttcttaaaac atgatgcaag aatggaagtt gaacttccag
     2461 acaatgttcg tttaaatgag gagtacggtc gtcggtgaac cattctggaa aattttccac
     2521 cagttttcgt ccaaaatcca tatcgacttt ttcctcaccg taaggcatca gaaaattgcc
     2581 cgaacagtaa acggaagcct tgtcaaaata cttgtgatga gaggaaccaa taagaaaaat
     2641 tcttttgtat tctctgtttg catcgatctg gttaaatgcc gaagcggtaa ttttgccgga
     2701 aaaaacataa ccggcatgcg gacaaataat ggctctgacc ctgtcgagct gtttgggtgc
     2761 agcatttttg aaaaaaatgt cgatttcttt ctgcaactct tcaacatttg ccggataaaa
     2821 cttgccggct actgcaggtc ttctgtcaat ggtttcgtta aatttttcca taatatcaag
     2881 tcaaggttaa gtgattgatt tactatgaaa aacaattcgt atttaaaaaa gttcaaagga
     2941 aattcaaata taataaaaat ctgtcagaaa aaaaataaaa acacgtgttt tttctgattc
     3001 atatttttat gaggttttaa gttttttgca aaaatgagtg aattcacttt ttgaaaatta
     3061 attaccatta attaaaaaat tatacacaaa aaaatatcgc ttttacattt tactttgcta
     3121 tatttgcgtc aaaatttggt tactgtgtgg tttaaatttt aaaatcacaa ctgctcgaga
     3181 aataagtttc gaccaaattt tacggattga aatttaatgg aatacaagct ttttggccaa
     3241 caaaagttaa ataccaaata tgaagttttg gaaactaaaa gcaaattaat atgagcaaag
     3301 gatgtaacaa tggtattgaa aatgaatgtt ttgtcggaca ataccgataa aattaagtta
     3361 aatttgatta atcaacaata cttggtatcc gattacaaaa taaaaatttt taaaatacaa
     3421 gaattaagtt tttatcctga ttaacaaatt tctccaaaaa attatggaca aaaaatacaa
     3481 tgcccttagc ttttctacca gcaagtttgt tacaaaattc tacagtactt ccttttattc
     3541 cgcttcgcaa tacttttcaa aagaaattca ggaagccatt ttcagcatat atggctttgt
     3601 acggttggcc gacgaaatcg ttgacagttt tcatttttcc gataaggaat atctgcttca
     3661 gaaatttgaa tccgattatt atgatgccgt gaaaaacggt atcagcctga atcccatcct
     3721 tcattcgttt caaatgactg tgggtaaata tggaatttcg gatgaatatg tgcagcattt
     3781 tctctctagt atgaaagccg atttggaaaa aaaagaatac aaaagtcaaa ttgaactgaa
     3841 tacttatgtg tatgggtcgg cagatgttgt tggattgatg tgtttgaaag ttttttgcaa
     3901 gggagatgag caattgttcg accgattgat gctgccggct accaaacttg gatcggcttt
     3961 tcagaaagtt aattttctta gagatattaa gcaggatacg caagagctgg gcaggcatta
     4021 ttttccgcaa attattgaag aagggttgac cgaacaaacg aaaaaagaac tggttgagga
     4081 tattcgaaaa gattttgacg atgcctacaa gggaatcgta ttattgccag ctgacagcag
     4141 actgggggtt ttgattgcct acaaatatta tacggcactg ttaagaaaaa ttgaaaaaac
     4201 tgctgcctgc gatattctca acaaaagaat cagaatttcc gatgccggaa aaatgttttt
     4261 ggttttgtca acgcaaataa aatattattt aagagccttt tatggaaaca aataaagaga
     4321 attttgttca gttggtggat gaaaatgatc agcctgtggg aatcatggaa aaatatgaag
     4381 cccacaaaaa agctgcttta caccgagcca tttctgtttt tatcgtgaac tccaaaggcg
     4441 attggctgat acatcgcaga gctttggaca aataccattc caacggactt tggacgaatg
     4501 caacctgttc gcatccctat cctgatgaaa gttatctcga agcagcaaaa cgaaggctaa
     4561 aatacgaaat gggaataaat tgcgaattaa ttccactttt tcattttatt tatcaggaaa
     4621 aagttaatga tgaactgaca gagcatgaat tggaccatgt ttttttcggt gtgagcgatg
     4681 acattcccat tcacaacacc ggtgaggtag aagaatggaa atacattccc taccgacaac
     4741 tgcaggaaga tgttgaaaat catcccgaaa agtacaccgt ttggttcaaa ataatattga
     4801 aaaaagtcaa agcatacatt gatacggaaa atttaacaga aagttcatgc agataagcct
     4861 tttacttcaa aaattcaagc agaaatatcc cgaaaggaaa gttcgacaat tcattttcac
     4921 cttttatgcg gtaggaattg ttggtttggc gctgccggca acgcatgttt tctttgttta
     4981 tttgatcccg ttggctttac tgctttcggc tttcatcctt ctcccttttc aggaagatga
     5041 ttttagtatc aaaacgggcg tattatttac tgttattttt gttgtttctt ttacagtgga
     5101 agctattggc gtaaatacgg ggaaaatatt cggacagtat ttttatgaaa acggtttggg
     5161 cgttaaatgt tttggtactc cgctgattat tggtttaaac tgggcattgc tggtttatct
     5221 cacggcttcc atttttcaat cgtttcgcat caacaaattt tttcttgtac tttttccttc
     5281 tgttttgatg gttttgtatg atgtggtact tgagcaggtt gctcccaaaa tgcaaatgtg
     5341 gttttgggag gcagaccgcg tcccttttcg gaattatttg gtttggtttc tcatggcttt
     5401 gatttttcat acattattgg taatcagcgg acttaaatta aaaaatcctt tgagtaaaat
     5461 cgttttttcg gctcaattct tgttttttct tatccttatg ttcatcctct aaaaaggttc
     5521 tatgttgaaa gccaaacatc attgcctgat ttatcctttt tttcgtcgat atacactttt
     5581 gttgatgaaa aggaattttc attcggttaa cgttattcga acaaattttt ctcggaacga
     5641 aaatttgccc gttttgatga tagccaatca tattagctgg tgggacgggt tttgggcaat
     5701 gtatctgaat ttgaaagtat tccacaaaaa gttttatttt atgatgttgg aagaccaact
     5761 cttgaaattt cgtttctttc agtggacggg cggattttcc ataaaaaaga aaagcaggga
     5821 aatgattgaa tcgttacagt atgctgccaa aattttggac aaccccgaca atttgctttt
     5881 aatatttccc gaaggaaaac ttcagtcgtt atacaatcct cttcacgttt tcgaaaaagg
     5941 aatcgaaaga attgttgaag gacacaaaaa taattttcaa ctggtatttg tggccaattt
     6001 tatcgattat ctttcgcaca aaaaacccga tttgtacatg tatgtgtccg aatatcaagg
     6061 aaatgttttt tcgcattcca agttgcaaga agcctataat gttttttacg gcaactgtct
     6121 cgatattcaa aaatctaaaa cagaataagt tatgatatac ttagcctggg ttgtttttgg
     6181 ttttactttt tttcaactgt gcattgccgg gttgaattat tttttccgtg aaaaaatacc
     6241 aatttcgaca aaccggattt ccgacgaaaa aatttcggtg cttattcccg ctcgtaatga
     6301 ggaacaacat attggaaatc ttttaaatga tttgtccggc gaagacgaca atattctcga
     6361 aattattgtt tttgacgatc tttcttccga tcatacagtt gaaatagtga atcaatttat
     6421 tggtacgaac gaaaaaatac ggctgattcg ttccacccat ttgccagcag gctggctggg
     6481 gaaaaatttt gcctgttatt cgcttgccaa cgctgcaaag ggaaattatt ttttgtttct
     6541 ggatgcagat gttcggatgg aaaaagaaat tgttgtttca tcggtaaaat ttttcaaaca
     6601 aaaatcgctg catttgcttt ccattttccc gactcaatta atgatgacaa aaggcgaaaa
     6661 aatgacggtt ccaaacatga attacatttt attgactttg ctgttgctac ctttggtaag
     6721 aaaattatct ttcccatcta tttcggcagc aaacggacaa tttatgctgt ttgatgccga
     6781 tacttataaa aaatttcaac cacatgaaaa atttaaagac aagcgggtgg aagatatcga
     6841 aattgcaagg ttttacaaga agctaaaact taaaattgcc tgtctcaccg gaattaaaca
     6901 agtaagatgt cggatgtatg aaaattttgc tgaagcggta aacggatttt ctaaaaatgt
     6961 aattcatttt tttggaaatt cttttttgat tgctgtttta ttctggattg ttaccacttt
     7021 tggatggtta gcagtatggc tcgcattcgg attaccgaca cttgccgttt acttgcttgc
     7081 tgttgtctgt atcaggatat ttttttcgct ggcaagcaat caaagtatat tggataatct
     7141 catttttttc attccccaac agttttctct cggaattttt atttatcagg cgctcatcaa
     7201 ccgctcgaaa aatcaatatc tatggaaagg aagaaaagtt tagttttatt ttttttgttg
     7261 attttgctgc atctgcatgt tcatgcttcc cacagggaaa atatttacaa agcctacatt
     7321 aacaacgaca tgcaggcttg gaaaaatacg attgataaaa tgaacctttc gtcaggcaat
     7381 gcttccgacg aatttcttct ggagctggta aattatgaat atggatacat tggttggtgt
     7441 attgggaaca aaaaaactgc agaagccaaa gaatatttaa aaaaagcaga aaacagacta
     7501 atcactctcg aaaaaaatca atttcgctta tcaatgattg aagcctataa atcggctttt
     7561 tacggatttc aaataggatt caataaatta aaggcacccg tgttgggacc caaaagcgtt
     7621 gaagctgcca aattagccat tcaatacgac ccaaacaatt actttggtta tctgcaaatg
     7681 ggtaatattt actattattc gcctgctgct tttggtggtt ctaaagcaaa agccattgaa
     7741 agttaccgtc aggcagaaaa gttgatattg tccaataacc accgacttaa aaacgattgg
     7801 aattatttga gcctgcttgt tactctggct caagcttacg aaaacacagg caatgacgaa
     7861 tcggcaaaaa tttactatca gaaaatctta aatgttgaac ccgattttgc ttgggtgaaa
     7921 aacaaattat atcctgaatt tttaaaaaag actcaaaaaa ataatatatg aaagaaaaag
     7981 tgatcatagt gggcgccgga cttggaggat tggcaacagg acttcggctg gcaaaagaag
     8041 gatacgaagt agaatttttg gaaaagaaca ccaaagccgg aggcaggtta aatcagatta
     8101 agaaagatgg ttttacattc gatacggggc caagtttttt cagcatgtcc tatattttta
     8161 aggaatttgc cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaacttgc
     8281 caaacaattc gatgatgtgg aaccggattt tgaactgaaa atggaaaaat atctccaaaa
     8341 atctgcacag ttgttcaatg acacggtaga tattgtgata aaaagaaatt tcgattccta
     8401 tctggattat tttcttgcgt tgatgaaagt taatcctgtt catttgccca tcttgttcaa
     8461 aactttctgg caacatgtat gcgattattt tacctcgaag caagcacgag aaattatttc
     8521 gctggtagct ttttttctgg ggaggacgcc gtttgataca atgggaattt acagtttact
     8581 ttcgcatgtc gaattccgac acgatggcta ttacaacgta aaaggaggca tgtataaaat
     8641 tgttgaagga atgctcgaag aactttctaa attggatgtc aaatttcatt atcagacaga
     8701 aattgttgca tatcatgccg aaaatcgaaa aattgtttcg ttaaaagatg ctcaaggaaa
     8761 tttatggaaa gccgacaagt ttgtgatcaa tgccgatgcg gcactgtttc gatcgaaagt
     8821 tatgaaatac aaaaattttt ctgaaaaaaa actggataaa atggaatgga caatgggata
     8881 tttaactttt tatctgggct tgaaatgcaa acttccgcaa gtaaaccatc acaattattt
     8941 tttggggtcc aactacgagc aatacgccgg caatatttta caaaatcccg acacactcga
     9001 aaaaccatac tattatgtta acgtggtttc gaaatataat cccgaatgtg ctcccgaagg
     9061 ttgtgaaagt ttattttttg tttgtcccgt tcccaatttg aaatacaaaa acgattggag
     9121 cgaccgcgag aaaattgtca acagcattat tgacgacttt tcacaacgaa tcaaaaagga
     9181 catttctgcc gaaattattt caaaaacgat atttacacct caggaatggc gagataaatt
     9241 ttccttgtat cagggtagtg gactgggact gtctcataaa tttacccaaa ttggagcttt
     9301 ccgaccaaaa aattacgacg aaaaacacga caatcttttc tacgtgggag cttccaccat
     9361 tcccggcgcc ggtctgccaa tggcagttat cagtgctgaa ttggtggtgg aaagaatttt
     9421 aggcaagtaa tcgaatcgaa aagtcaattt ttgttaaaac atttccggca tttctctgtc
     9481 ttaactcgtt aacaaaacag aaaaatgccg gataaaaaat tttttcattt ttcggatttt
     9541 actccaaaaa aaatcagaaa atatattgtt cgctgataga tttatgttcc tgaacgcggc
     9601 aaatagtgtc ggcaaacata tcggcaatgg aaagtatttt aactttggaa cattttgagg
     9661 catcaaacgg gatggaatcg gtaaaagcca attctgtcag tgccgatttt tcaacttttt
     9721 cggaagcact tcccgacatc acgccatgag aaacaatggc acgaaccgta ttggctccct
     9781 gttccatcat taaattggca gccatgcata aagttcctgc cgtgtccacc atatcgtcaa
     9841 caataaccac atcttttccc tttacgtctc caataattct catttctccc accacattgg
     9901 ctttttcgcg cgttttgtgg cacaacacca ttggaacgtc aaaatacttc gaatacgaac
     9961 ctgcacgttt ggaagcaccc acatcggggg cagctatcac cagatttttc agacgcaaag
    10021 tttgaatgta cggagcaaaa attgttgatg catacaaatg atccaccggt acattgaaaa
    10081 atccttgaat ttggtcggca tgcaaatcca tagtaatcag tctgtcgata ccggccacac
    10141 tcaacatgtc ggcaataagt tttgctccca gcgaaacgcg tggtttgtct ttcctgtcct
    10201 gacgtgccca tccgaaataa gggataacgg caataacttt ataggctgaa gctcgttttg
    10261 cggcatcaat catcagaagc aactccatca aattatccga attcggaaat gtggattgta
    10321 ccaaataaac atttaatccg cgtatcgatt cttcgtaaga aacagcaaat tcgccatcgg
    10381 caaaatgctc aatgttcatt tttcccagcg ggcatcccaa agattgacag attttttcgg
    10441 ctaaagaacg actgtttgtt ccggaaaaca ctttaaatga ggagtttgaa gctgccatga
    10501 ataattaatt tttgagttaa atatttttcg caaaattact cattttatta gtaaaaacag
    10561 acttttgatt cataaaaaat ttttctcttt tcaaatcatg tttcacatga cccaataatt
    10621 gctactaaaa acaaagaatt ttatttttgg aattttataa attaaaattg gtgcaaacgt
    10681 ttgtataaat ttttgtataa ttatttacgt caacaaacat cattttaata gaaataaatt
    10741 tatttttgaa gttgaaaatg ttaatgtagt tgaaacactt atttctaaat tatgaaaatt
    10801 cattttaaaa taccttacta cacccattgg ggacagcaga tttttgtttg tggcaatctt
    10861 ccggagttgg gaaacaatgt tcctgaaaaa tcgtttcagt tacattttca aaataccgaa
    10921 gactggtatg gagaaataga gattcatcaa agtgaacatt ttgagttgga gtaccgatat
    10981 ttactttacg aagaaaacac aaaacgtttt acttacgaat ggattgaaga tcgtaaaata
    11041 accattgacc caagaatgga gcatattttc tgtttcgatt tttggaatgc tcccggagcg
    11101 gtcgaaaatg tattttttac ggctcctttc aaaaaagttt ttttcagcga caatttcaat
    11161 cactcacaaa aaactcgttc aaaaaagaat tttacccata ttttcagaat caaggctcct
    11221 ttgctgaaaa atcatcaagt tccttgtctg attggagatt gcaaagtgtt gggcagttgg
    11281 aatacggttt cgcccttact tttgaccaga gacggtgaga attggtggaa ggcagaagtc
    11341 gatctgggta aggaaaagtc ggacgttcat tataaatatg gaatttacga caccgaaaac
    11401 ggacagtttt taatgtttga aaatggtttt gaccgaacgg cacccgtatt caatgcaaaa
    11461 aacacatttg ttcgtctttc agacggtttt ttgcgaatga attcgcaatc atggaaagga
    11521 gccggtgttt cggttccggt tttcagtctg cggacaaaag agagttttgg tgtaggcgat
    11581 tttggggata tcaggttgct tgtggattgg gctgaaaaag ccggtttaaa gttgattcag
    11641 gttcttccgc tcaacgatac cattggtacg cacaccgatg ccgatattct tccttacgct
    11701 gccatctcgg cttttgcgtt gaatccgctt tatcttaact taccctcgtt gggaaaactg
    11761 cccgaaaatc atccgttgca aaaagaatac gaggagcgac aaccgcaatt gaatgctttg
    11821 gaatggattg attttctcga agtggtcaat ttcaaactga agtatgttaa agctcttttt
    11881 tcttttcatc aggaagagtt tttgcagaat aaagattttc tgactttttt tgaaaacaac
    11941 aaacactggt tgattccgta tgctgctttt tgtgttttga gagaccggta tggtacaccc
    12001 gattatcatc aatggaaaga attttccact ttcaatgctg aaaaaatcgc cgcatttgtt
    12061 tcggcaaatc agccccatta cgcagatatt gccctgaatt atttcattca gtatcatttg
    12121 catatccaat taatggaagc tgcttcctat gctcatcatc atggcattgt gctcaaggga
    12181 gatattccaa ttggcgtcaa taaaaacagt gttgaaacat gggcgcatcc ggaacttttc
    12241 catattgata tgcaagccgg cgcacccccc gacatgttct ccataaaagg gcaaaattgg
    12301 gaattgccaa cttacaattg ggaagctata aaacaaacag gatttgactg gtggaaaaaa
    12361 agattgaaac aaatgtccaa ctatttcgat gcttttcgta tcgaccacat tctcgggttt
    12421 ttccgaattt gggaaattcc caatgaccag ctggagggta ttatggggca tttgtatcct
    12481 tccatccctg tttatcttga tgaatttaaa gcaaaaggaa tttggttcga ttacaaccgc
    12541 ttctgcaaac catttattaa cgatcaggtg ctggagcagc tttttggaac tgatgcgggt
    12601 tgggtaaaag agaattgttt acaaatagaa gatggctgga tacttcgatt aaagcctttt
    12661 tatcgcagtc agaaaaacgt tcagcaattg tatgaagaag gacaattttc ggaaaatatc
    12721 agaaacggat tgtttgatct gatttctaat gtgctgtttt ttgaagtgga aggttcgcat
    12781 ggaacgcgct tttatccgcg ttgggggatg cattcgctat caactttcca atatctcgac
    12841 gactttacca agcaaaagtt ggatgaaatt tatatagatt atttttatca caggcaagat
    12901 gaattttggt ttcaatcggg aatggaaaaa cttcccgtgc tcaaacaaac aaccgatatg
    12961 cttatttgcg gcgaagattt gggcatgatg acgccttgcg taacccgagc catgaaagaa
    13021 ttgggaattt tgagccttga aatacagcgt gctccgaaaa ccaacaaaat agagtttttc
    13081 catccgagcg atgcgcctta tttgtcggtt gttactcctt ccactcacga tatgagtact
    13141 attcgaggtt ggtgggaaga agacagaaac ataacgcagc gtttttacaa tacacaattg
    13201 gggcattggg gcgaagcacc ttatttttgc gaatggtgga tatgtcggga cattctgctc
    13261 caacatgttt attcacccgc tatctgggca atttttcaaa ttcaggattt actgggcgtg
    13321 tcggaaaaat tacgaagaga aaatccccat gaagaaagaa ttaatgtgcc ttccgattcc
    13381 aaaaaaagct ggcgttatcg cttgcatttg aatttggaag atttactgaa agaagatgat
    13441 tttaatcggc aaatcagaaa ttacatccgg caagcgggac gataaaaaat tcttgatttc
    13501 tattagcgaa atcttacagg gaaccattct tgaagacaat ttttggttag acaaattttg
    13561 taataaaatt catttgttgt taagaaaccc acatgtattg catacacaaa acgaatgaat
    13621 ataatattca gacatgtggg cttcatcaga ccattaaaaa ataaattata ttctgatgaa
    13681 aaaatttcgt gaattatatt atttgaaatc cactagcaag cgaaggttca gctgccggcc
    13741 ggtaagataa ttgggaacag ccacctgatg ctgataaata tctgttaccc agtagtaaga
    13801 atttacattc ttaaaatcca gaagattaaa cacttccaca ttgatccaga tgtttttcac
    13861 atgccccagc cacgatttgt tcatccattc atccgaaccg tttaccagca gccgtgaacc
    13921 accaatatcc acacggcgat aaggaggcat gcgaaaagcc gaacgaaatt gttcgttgcg
    13981 tggagcgcca aacggtaatc cgtcagacca aattaacttg agctgtaatt tgtattttgg
    14041 atttgagggc aaataatcct gaaacatcat cgaaaaagca tagcgttgct ccgtaggacg
    14101 aggcacccag gatggatata ccgtttcgga agtcaaaata ttgccttcgt cgtcgtaagt
    14161 atgacgcaca tagctgtcgc caataatatc ttccttcgaa tccatcagcg acagattgat
    14221 ccacgaatcg gttccgggaa caagttctcc gaaaagtttg aaatcgatgc cggcagtata
    14281 agctttggcg tcattttcac ccgaatagcg aatgcgaaca ttatccaccg aataggaaac
    14341 aaccctgtct gccaacttaa gataagcttc ggtagtgaat ttgaaaggtc ttccccacgc
    14401 cctgaaatag tggtcgccac ccaacacaaa atgcaacgaa cgttgtgcct ttatgttgga
    14461 attcaatttg atatccacat ttcccaaagc atcggtaagc gtatcgcgaa tttccttgta
    14521 aaaaggagcc tgataataaa gccccgtagc aaaacgaaaa ctgaaatctt tctcccaatg
    14581 cggaagatag gcaacagtaa aacgcggact gagtaaaaat tcgcggttga aagtccaata
    14641 atgagaacga agtccgccgg taaatgaaaa agttccggca gtggaagaat atttataggt
    14701 atcctgtaaa aaggacgttg ttctaagcgt tttcagcgaa ttggaagcat tcatgttgta
    14761 atacaaattc acttgcctgt tattgtaagg caaagaatag ccggccgagt cgcgccattc
    14821 ccattcattg atctggtcat cgattttttc tatctgaacg ttggcacccc acttcagtaa
    14881 atgaccattt tttgcgtatt cgcccaaatg tgacagattg gcaaccgtag cattcagctt
    14941 gtttcgggcg tgttcgtgat aatttccaat acccaaagat tcgccggttt gtttatcggg
    15001 gtcaacatcc attttaactt cactcaaaat atattcgccc gagatgtcgt aattctctct
    15061 ttcgttggtg taaaatgccg aaccgatgag acttagtttt gtcgattttg aaggtctaaa
    15121 attcaacgtc aacgcaccga aagaagtttg aaaaaggtct ttttcctgtc cttcaaaaaa
    15181 aatggtcagt tttcgtccca tcgaataggt gccaaatgat gtttcgcgag tctgaggaat
    15241 aaaacggtag tcattttgtg aaaaatttcc cagaaatgtc aattcccatt tgggttttat
    15301 ttgataggta agaaaagtct gataatccac aaaagaagga tcatattctc cctgagtatc
    15361 cattgttccg agcaaatact tggaagtttt gtaacgaatg ccgtgcattt gtgaaaaccg
    15421 cttgccggcc gtacccacat aagccgaagc cccgagcaga ctggcagagg ctgttgcttc
    15481 aaatgcagaa ggttttttat agctgatatc caacacggaa gacattttat caccatattc
    15541 ggcattgaaa ccacctgacg aaaaggatac attttgtacc atatcgggat tgataaaact
    15601 caaaccttct tgctgtccgg ctctaatcag caaagggcga taaacttcaa taccgttaac
    15661 atatacaata ttttcgtcaa aatttccgcc tcttacgttg tattgagaac tcagttcgtt
    15721 actggaactt acgcctgtaa aagaaatcaa cagcgattct attccgccgg ccgaattagg
    15781 catcaagcga tatttggaag gatcgaccac atcaatggta gatgtctgcc gtcgctggcc
    15841 tattacattc acatcttcaa tttgtttcga tagcggagga agttccacag ttaactgtat
    15901 caccttcaac ttaggatgaa cggtataggt aaccgtttga taaccaatca tagaaaaaac
    15961 gagctttatc gaatccttga tgtcgaccgt tagttcataa tagccattct gattggtaga
    16021 agtgccaatg ttggtatgtt gaacatatac atttgccaat tctatgccgc gattgttggt
    16081 atcgattaca taaccgtaaa tacgcacttt attttgcgaa aaaagcgaaa tggatagtat
    16141 tgaaaagaaa acaaacgaaa ataatttacg gctgtgcatt gttattgtct tcacactaaa
    16201 attaatactt ttttaaacgt cggaaacaaa agtttatttt gttggatttt tgaccaaaaa
    16261 acaaatagaa agcattggta gaataaagaa aagctacccg aatttggatc attcctctaa
    16321 tttgtagctt tttgatcata caaaacagac tataaaagta tgttgcccac aaagatatta
    16381 atttataagc tgaatttaac ttataaagga aatttttagt gagacaaatt tcgtactttt
    16441 gttctaaagt tctatgcttc attttgataa ttttttaggg gaaaagtggc aaggtaaaat
    16501 atatacttta caaagcagca aggattttgt tgcttgtttt gcgctaattt ttctttacct
    16561 tttcttttcc taaaaagttg cattattcag ttgactttac gacaaataaa tttatacata
    16621 tgaaaatcag taacttatta gtaattttag gcttattatt ttgtttttct tttgtacaag
    16681 ccaaaagcat tcaaaaaata acacgtactt atgccgttaa ggattctttg gaactgaaaa
    16741 tggatattta tactgtcgat tccacatctt cttctttgca accatgtttg gtatttgtat
    16801 ttggtggagg gtttaaagaa gggaaaagag acgaaccgac ttaccaacct tattttgatt
    16861 attttgcaaa aaaaggtttt atggtggttt caattgacta tcgattgggg atgaaagacc
    16921 aacgctcacc cggggttttt aataccaaac ctttgcaaaa tgccatttct ttagctgttt
    16981 tagacttata ttctgccaca aaatttttaa tagaaaaggc tgattcattg aaaattgaca
    17041 aaaataagat cattattagc ggttcaagtg ccggtgccat tacagttctt caggctgagt
    17101 acgaattaaa gaatcatcat gctataacat ccgaattacc tcacgatttt cgttttgcag
    17161 gcgtaatttc ctttgcaggg ggcattttca gcacgaaagg catgccgtct tacgctgaat
    17221 cacctgcacc aaccctgttt tttcatggta gtgccgacaa actggtgcca tataaggaaa
    17281 ctcgactttt taacaagggc atgtttggtt ccagatcgtt gaccaacaag tttcataaaa
    17341 agaaatattc ttatcttttt tacagcatgg aaggtttagg acatgaagtt gcaagttttc
    17401 caatgaaaga atttttacct gaaattgagc aattcattca aaattttgtg tttgaacaaa
    17461 aaccctggat ggttgaagtt tattttaccg acaaggagcg aaaaacagaa aaatcagtta
    17521 ctccggccga ttattacaag taatcagttt gccgaaaaac gtttttcgct atttatactt
    17581 tcatcatttt aagaaaactt ttgttcaaaa tccaaagcta aagccggcag taaaagtagt
    17641 ttgtttaccc tgaagaaatt ttggcgtata gtgattcaat gcttcttcgg cagtcattct
    17701 cctttcacca aaagtaactt ccgaaacagc attaaaagtt tgtatatagc tgttttctat
    17761 gttcaatgtt gcataagtat tggggaaaag ctcatatttt acatggagtg ccagagtttt
    17821 attgctccaa attttgtctt tcataaacgg ttgactaata atgtcgagaa cacttgcttt
    17881 tgagccttgc caggttccgt gtcgaatata ggcatattcg tttccgtgtt cggcttgtgt
    17941 ccacgacagg tttatgtcca gcccgcgcat aggtttgtat ttaaaggcgg cataaatttc
    18001 ctgcgaattg tcgcccaagt aatttcccag attgaaacca ttggttgccc atgtcagagc
    18061 aggaatagaa tgtttgtaac taaggatggc attttttgtg tattcacccc tcaacgaaag
    18121 attttccagc gggaaattgc taaggcgaaa tcccagttta taggaaatag gatttttttc
    18181 cttgctggag ggtttaatgc gggaaatgga gaattcgtcc acaaaaatgg aagtaaacaa
    18241 gtgcaaatgt tgtatgtttc ttgaactgat attaagaaac agttgcgaat tctgattttc
    18301 ggtacccata gtcagcgtat gatccatcga tttgtaaaaa gctaaaggaa tcaaataagc
    18361 gggctgtaca ttgctttcgg catacacaat ggcatttccc gccgacagat gcaattgggg
    18421 caggggaata aatgatatca tattggcagc cagaaattta ttggtgtaac gatacatttt
    18481 ttcattttga ttggaaagat aataacgtgt gctgtcgatt acattggaag tcagccaccc
    18541 atgaaaataa ttcatttcca gccattttgc cggtttcagg ctcagcttca gcatgggaaa
    18601 cgaaggcgtg cgacccgcaa taatgttgga accgtgatag ttgtcgcccc attgcacatg
    18661 gtcttttgtc agtgcaacac ttccccattc ccaagcatac gaaataccgc cacgcatttc
    18721 gctgtaatcg ccaccattgg tagtacgttt gtaggctccg ccctgatacg gtgtcaggta
    18781 agttgattcg gcaagcaatt ctccctgttg cgaaacatcc cggagggaag catagacggc
    18841 aaaatgtttt cctatcatcg attgaaaatc aattccaaac cagcgttgcg taattttgtc
    18901 gttgttgttg tagagaatat tcattcccaa aatgggcatc acacgtccac gtaacagtga
    18961 atcgcggtag tgaacggaag gagggaaaac atccaactgc cagttggttt gttcgaaaag
    19021 cggataatcg tattgcggga ggcgattttg ttccaacgca taattttcca gaaaaaaata
    19081 caattctttc cgctgacggc ggttgagttt tgcttccttt gattttgcct gttgcaacaa
    19141 ttgggctata aaagttcttg aatagggctt aacaaccgag ttgatttcaa taagtttttc
    19201 gttggcgagc tcatccagaa aatcgtaaat gcgggagtag gaaatatgtt cgggaacatc
    19261 ttgagcaaca acaaaaagga tattggaaaa gagcagcagt gcgataagta tttttttttg
    19321 atgttttttc atgttgtagc caaattttta agatttttca aaattagttg aaaataaagg
    19381 caatatgttg ttattaaaaa attttttcgc taaagaagtc ttgaaatttt tttgaaaaaa
    19441 gtttgattta acctgttgct tattttggtt ccttatactt ttgcttttta taagttcttg
    19501 gattcttgaa ttttcaggca cttcgtgcat gatgaaacag aatattcttc agaataagaa
    19561 agaaatattt tatatcggtc cataaaacgc catttttttc gcaggccagt aaatattttc
    19621 gttccgattc ttgaatttca tccaacgtgc gaggcatatc ggcatagaaa gggggtagca
    19681 atccgggtcg gtgtttcatg cgaagttgtt gaatttcgtc attgtacagg ttaaggtatt
    19741 gctggctaag tgggcgaatg ccaatcaatt tcatttcacc cctgaaaata ttgatcagca
    19801 tgggcaattc gtccagaaag tatttgcgca tgaattttcc gatggtggtt acccgaaaat
    19861 ctttgttgaa ttttcctcca tcttgtaaag aatgccgctg ataaacatat tcctgaagat
    19921 attccgaata gggatgcatg gtgcgaaatt tataaacata gatttccttt ccgtttttgc
    19981 catagcgttt caatttaatc aatgggccat aggcacgatt gtaaacgggt gtcggatttc
    20041 gtttcctttg cgcccatacg tacagaagtc catctatttt tttctcttca atcacctcaa
    20101 aaccgtgaca ataaaatctc cccaatactt ctgctttgga aaggatacgg ttttttccac
    20161 ctttcagcga atgataaatg cgcctcgaaa agaaaagttt tggaataaac cgcttgaaaa
    20221 aataatctaa agaatataaa atataattga tacccggagg atatttttga aggaatttct
    20281 tttttctggt gcttttcttt tcaaaacaac aaacaaaatt tccatcgtct ggcaattttt
    20341 cgttgatacc cgaaaaaaaa gtgttgattt tttccgtatc gttcaactta cgcaggtaga
    20401 taaaagttga ataatgataa tgaggaatca gttcaagatt ttcaatgctt gtatttaaga
    20461 aaaccctcac atcatctttt ctcaagtcga aacggctttg caaatatttc agcgcctttt
    20521 tacctgaata ctgctcaatg aaatttataa gattgaagta actttcttca tcaatttcac
    20581 ggggttctct tggttttccc tgaggacgtt ctttctcgtc ggaaatagaa atatcaaatt
    20641 gagtggcaaa tcggtaggaa aaataaaaaa acaaataaag aaattcaaca gtgaaaatac
    20701 caatggttac cgccaaaata acaaaaccgg aatattgttt gaaaaaatgg tgtagaatgg
    20761 ttacatatat gacgaactgc aaagaaccga ccagtaaaag tttgaccaaa gcctgcgagt
    20821 aagtttgttt tcgtaacgga aggtaacgtt gaaaaaaata tgaaaaaatg agccagcttc
    20881 ctgtgaatag gagagaggga ataaaatact tctgaaaagg aacaggcgtg gtcagtggaa
    20941 accagccata tataatgaga aaattaaggc tgagcagtat caaatcaacc aaaattgagt
    21001 tccattttgg tttg
//
LOCUS       DAOTAX010000033        20993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2060_length_20993_cov_31.983666, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000033 DAOTAX010000000
VERSION     DAOTAX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2060_length_20993_cov_31.983666"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1248)
                     /locus_tag="PLM04_05020"
     CDS             complement(40..1248)
                     /locus_tag="PLM04_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HON02469.1"
                     /translation="MSLNDQLHKQRSLFPEECLLKEIANQPRLSIGIPKENASIETRL
                     ALTPEGVAIVTEEGHSVYVERGAGEPMSYSDLQYSEAGAFLVDSPQDAFSADIVIKIT
                     PPTLDEINCMNDHASIFSMLQLSNLSTESIKAMMSKKLNAIAYELIRDEQKGFPVVSS
                     ISEIEGNTAITLIAELMSNQHGGKGLLLGSVAGISPPEVVILGASITGTVAARTALAL
                     GAQVKIFDHDINKLRTIQHHLGQQVFTSVIHPAVLFKALATADAVIGNLRYINGSERF
                     MVSEDLVKTMKRGAIIVDLSMDQGGCFETSECRTLQNPIYEKHGVIHYCIPNISARVA
                     RTSSMALSNILSPILLKIGHSGCINAAIAESSGFRHGVYVYKGFLVNRLIGDYYNIKS
                     NDINLLLAGY"
     gene            2167..3000
                     /locus_tag="PLM04_05025"
     CDS             2167..3000
                     /locus_tag="PLM04_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02470.1"
                     /translation="MKSIQLSTVFFLSIIFLVACNSRGKQEKEADNQLQKIQQLIQQN
                     ELNAAKIAIDSFHANFPRLVAKRRIAEAFQDTITRRECKRTIAYCDSILPFKQKEADS
                     LLLNFRLEKNEKYQDIGNYVYKTQSTEANAARTYLKAYVDENADYYLVSQYMGPKIEH
                     TSVEISNGEVFAHTDTIDITSASYLSFSDETGRWEIVTFKNEAENGVSEFISQYMNEN
                     LKVILHGKKNFSYFLQNNDKKAIKETYHLWIVKKDINRLKKEIEKAKLKIEQINRRNP
                     V"
     gene            complement(3010..4059)
                     /gene="mutY"
                     /locus_tag="PLM04_05030"
     CDS             complement(3010..4059)
                     /gene="mutY"
                     /locus_tag="PLM04_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HON02471.1"
                     /translation="MNLSDQLISWYQVHKRDLPWRNISDPYRIWISEIILQQTRVNQG
                     LDYYLRFVERFPDVETLAAAEEDEVLKYWQGLGYYSRARNLHHAARQIMQDFGGVFPT
                     TYSEVMQLKGVGAYTAAAVCSFAFNLPYAVVDGNVYRVLSRLFAIETSIDSHSGKKEF
                     DALAQQLMENSVPSLHNQAIMEFGALQCVPVSPDCSVCPLRVYCKAYEMKMVERLPVK
                     SKKTPIKQRFFNYLVINYNHHIFLQKRTADDIWKNLYEFPLIETDHLLQVQELLENEF
                     FCQLFQGLEQVKIINISKPVHHLLTHRRISAQFITLQIAEKNEALNQFIEIPNVEIGD
                     YAVSRLVEMYLKKEI"
     gene            complement(4405..6444)
                     /gene="metG"
                     /locus_tag="PLM04_05035"
     CDS             complement(4405..6444)
                     /gene="metG"
                     /locus_tag="PLM04_05035"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HON02472.1"
                     /translation="MKKFKRTLVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKGE
                     EVIFIGGSDEHGVPITIQAKKEGISPQEIVDRYHFLIKKSFEDFGISFDIYSRTTSKI
                     HHELASDIFKTIANKGGFIEKTSEQYYDEEAGQFLADRYIVGKCPHCGNENAYGDQCE
                     ACGSSLNPTDLINPRSAITGNIPVLRETKHWYLPLDQHEEWLRHWILEEHKEWKPNVY
                     GQCKSWLDMGLQPRAVSRDLDWGIPVPLERAEGKVLYVWFDAPIGYISNTKELLPESW
                     EKWWKSEDTRLIHFIGKDNIVFHCIIFPTILKLEGTYILPDNVPANEFLNLEGDKIST
                     SRNWAVWLNEYLEDFPDKQDTLRYVLTANAPETKDNDFTWKDFQARNNNELVAIYGNF
                     VNRTLVLTQNYFDGKVPVRNELQEIDLQTINELSDIKGNVEKLLDNFKFRDAQKEAMN
                     LARLGNKYLADTEPWKIVKTDKDRVATILNLSLQIAANLSIVFEPFLPFSSEKLRDLL
                     NLETFDWNNLGSIELLKPQLQLKKPVLLFDKIEDSAIEYQVQKLLNTKKKNELSAFKP
                     KDVKSNISYENFEKLDIRVGTVLACQKVPKADKLLQFKIDDGLGGRTIVSGIAQYYPE
                     PEKLIGQQVCFIANLEPRKLKGIVSEGMLLSAEDADGRITLIQPQTTVVNGAEVK"
     gene            6860..9202
                     /locus_tag="PLM04_05040"
     CDS             6860..9202
                     /locus_tag="PLM04_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HON02473.1"
                     /translation="MNMQLSILKNLRFSGVFLISIFSVFISYSAPRYDLIPYPQELKP
                     QSGEFILNKKTVILCPFDQPQIVEIAQQFSDHFALVAGIKLPLRDYQTSSTNKNFILF
                     NTGSLTNENPEAYTLKISTSSVQITGNTAAGVFYGLQTLYQLFPCEVYASKESEKIKK
                     WSVPCVEITDFPRFSYRGLHLDVSRHFFPVDFIKKYIDLMAIYKFNYFHWHLTDDQGW
                     RIEIKKYPRLTEIGSKRNETLIGHYYESFPQQFDGKPYGGYYTQAEAKEIVAYAKKRG
                     ITVIPEIEMPGHAQAAIAAYPFLSCTQDTTIKVATKWGIFKDVFCTRDTVFNFLEDVL
                     SEIMEIFPSKYIHIGGDECPKDRWKECSYCQSLIKKLNLKDEHQLQSYFTQRIEKFIN
                     EHGRQIIGWDEILEGGLAPNATVMSWRGKIGGIEAARLSHDVIMTPTEYCYLDYYQAD
                     PEFEPLAIGGFVPLSKVYNFEPIPDELTPAEAKYILGAQANLWTEYILDEKQVEYMAY
                     PRATALSEVVWSNKESKNWENFKERLSTHFQRFEKLGVNASHAFYEPQFNAQVTENNK
                     LKITLSCDHPKAKIYFSLNEKNPDQHYSKPLIIDSTTIVSVATFVDGKKASRTLQKTF
                     IVSKLTNLICQPDSNVSYHQQSIFKLTDGKLGNIKTTSEWARLISNEDYLLNIDLQKN
                     QIIQRFTMGILKAPAFCGQIPSEIILYGSKDGINYQPLEEQKFSSSFTGKYEIIRPVF
                     NFPPVEVRYLKIVLKNPRFCIENLENEKNNSIFIDEIAVW"
     gene            complement(9408..9530)
                     /locus_tag="PLM04_05045"
     CDS             complement(9408..9530)
                     /locus_tag="PLM04_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02474.1"
                     /translation="MSSVSMCTFNPPFLFPSPLGFPPIRFIISGKSMIVVESNA"
     gene            complement(10207..11124)
                     /gene="metA"
                     /locus_tag="PLM04_05050"
     CDS             complement(10207..11124)
                     /gene="metA"
                     /locus_tag="PLM04_05050"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HON02475.1"
                     /translation="MPLNIPKSMPAVEILRQENIFVMDSERASSQEIRPLKIVILNLM
                     PLKITTETDLIRLLSNSPLQIEIDFLQLKSHTPKNTPIEHMMAFYKTFDAIEASNYDG
                     MIITGAPVEQLEYEEVTYWPEVQEIFDWSRKHVTSTMFICWAAQAGLYHFYGIPKYPL
                     EKKMFGVFEHYIHNPQNPIFRGFDDIFYVPHSRYTEVRAEDIKKNPELILLSESDESG
                     VYMVMGRNGREFFITGHSEYSATTLDNEYKRDLAKGLPIDMPKNYYRDNNPANEPLVR
                     WRSHANLLFTNWLNYFVYQETPYDLSKIH"
     gene            complement(11207..12010)
                     /locus_tag="PLM04_05055"
     CDS             complement(11207..12010)
                     /locus_tag="PLM04_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HON02476.1"
                     /translation="MRKGFRITIYFVAVLLLMAGAWAVFHEKPVHDLPAMLKSGKLYV
                     VTDSSRMGFRLEGDSVYGFQYEIVKTFADQLGLTLVITPENNFETCLKGLTNGDYDIY
                     AGFIPVTTEWMNDIAFSDPLNTSRLVLVQKNFPDSLKRLMKNSQQDLAGDTIFVPKMS
                     PHKLRLKHLSDEIADTIHIVEVPNVNLEQLVEWVAQGKIRQTVCDEREARFLKLTYPQ
                     IDISLPLGFDQHYAWMVDKESKLLLQQLNDFLKDFIGSSAYWNIYRKYY"
     gene            complement(12060..13919)
                     /locus_tag="PLM04_05060"
     CDS             complement(12060..13919)
                     /locus_tag="PLM04_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HON02477.1"
                     /translation="MFILFIVTALSVWAINPPSIRYVKTWKVSDPLGMADSVQVDTLH
                     LNFQDENPIDRFSIANSYNGNLGSPIQSKLYFSRPESNDFIFSDAFYPYITDMQSAVF
                     YNTKTPFTKIVYNTGGSNYRQEDQIKFLFTFNANKRLNFGTTLDYIYSVGEYANQAAK
                     RFTGSLFGSYDGKHYSAVGLAAVNNMFNHENGGISDSSYIYEPIGVKTQDIPVNIHGY
                     STYRKNVFFYHQKYTIGFDQIIRISPDSVRKEYVPVTHFGHTIKYEDPRKRYYEANVE
                     KDFYKNTYLPFTQTNDTAALQTLTNTVSVGIAEEFNKWMRFGLTGFVENELQRFTYNV
                     DSLIQKKNESNTKVGGILSKQRGERFRYNILGDIYLLGYKQGDFSIKGQVNGFFKAWK
                     DSIVLSANGFVRSDEPSYFLNYYQSNHFRWENNFDKIYRTNAGGTFSIPTRKFSVNVS
                     VENLTNYVYFNSEALPEQYDGNIQVIAANLKKDFHVGNFVLENNVVYQLSSQERVLPL
                     PTLTLFHNLYFKNMLFNVLSIQLGANVRYHTAYYAPCYMPAIAQFYTQSETKVGNFPV
                     VNVYANFHLKQARFFVEYFHVNQLFMNHAYFSMPNYPINPAIFKIGVATNFYN"
     gene            complement(14104..15192)
                     /locus_tag="PLM04_05065"
     CDS             complement(14104..15192)
                     /locus_tag="PLM04_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HON02478.1"
                     /translation="MKMNNFFKYFSFYALIFGFFLFSSLNLWASNEKKINVKIACVAF
                     YNQENLFDTIDNENVNDYEFTPNGPNRWNSMKYKSKLEKMSYAISQIGLDVTPDGAAI
                     IGVSEIENRGVLEDLVSQPSLKKRSYEIVHYDGPDRRGVDVALLYNPRYFIVSNSKSY
                     RLHTADTTFRTRDQLMVSGYLFGEKIHVIVNHWPSRSGGEMRSRPKRNAAAALTRSIV
                     DSLYRVDKNAKIIVMGDLNDDPFNESVAKVLGAKKNASEVKETELYNPFWKILDRGIG
                     SLAYNDQWNLFDQIIISAPLLNAPPSELKFWKAEVFNRDFLIQQEGRYKGNPWRTFAG
                     GVWLNGFSDHFPTLIYLIKEVDSQANEN"
     gene            15581..18310
                     /locus_tag="PLM04_05070"
     CDS             15581..18310
                     /locus_tag="PLM04_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON02479.1"
                     /translation="MKKLFLILFVFFLCIPLLMAETSIKGTVKSADTQNPIPGVIVSL
                     IGQNISTQTNAEGQFSLRFIEAGDQEIRISKIGYFTQIKLIHIEPDKENDLGTFYLRE
                     DVQQQTKQEVVLQLSENQIDEDEGRATQNISAALSSKGDVYVSQSSFSFSPMRFRTRG
                     YDDNFESTYINGVYFNSLERGGFNYSSLGGLNDAMRNQDYTYGFAPNSFSYGNLGGTT
                     NINTKASNYAAGTKASVAYTNRSYKLRGQATYATGLMQNGWAFAGSAVVRWADKGIVD
                     GTFYNSWGYFFSAEKILNKQHSLSFVTFGSPTQRAQQGAVTQEVFDLAGSIYYNPYWG
                     YQEGKIRNSRIVKSFDPTAILSHEYKISNHQLLRTGLAFHYSLYSNSALNFYNAPDPR
                     PDYYRNLPSFVSDDSLKNVIANQWKSDTNVSQVNWSALYQANYKNNVINPNGTARYIV
                     ERRHNNLMEGALNSTYTQQVNKQLKLIAGIEGKMSKGMHYKTLDDLLGGNQWIDIDQF
                     SERDFPSNPDIIQNDLRNRNAVIKEGDTFGYNYDINMAYANGFIQNEWILPQFDIYYA
                     AKLTYTQFYRYGHMQNGRAPENSYGKSKTWYFVDPSFKAGFTYKIDGRNRLSFNGLIE
                     SRAPLASQAYLSERIKDMLIPYLQSQDIRSADINYNFTYSTIRGRIGVFQTNLYNTSD
                     MLGYYDDEYRTFINHVLAKSDKVYKGVELGTTVKINSNFSVSLAGTYADYHYANNAIG
                     VKSPENGAFADVAETVLTKGLKIAAGPQAAGNITIDYFHPKMWFADVTLNYFDNNYLD
                     FAPNRFTENNMAKYTTPEMMAALGTQEKLKGGFLLDASVGKLIYLKHRRSLNFNLSAS
                     NILNNTKMITGGYQQARLPLSNGAIDPTGLNRFPNKYYYAWGFNVFFNIGYKF"
     gene            18355..19341
                     /locus_tag="PLM04_05075"
     CDS             18355..19341
                     /locus_tag="PLM04_05075"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HON02480.1"
                     /translation="MKNFKIGGISILLAAIFTSCAETDFDAIPVNKLMGDSLTTTYTI
                     EQLKNNFLTLNDAYSDTVNYKGGLYTADPIQAESDIVISGYITSTDVDGNVYKYFVVQ
                     EPGANGQAIKVSIDASGLSAIYPLGQKVWIRCNGLYIGRYGEAEQIGSRYVNKERFKV
                     KKSTGDTIYRIEPGRMPFLIANEHIHAYGMPDPTVIKPDTMTIAQIKASNHTKLVNKL
                     VCIKNAFFTGKSEGDNLTDSELIFAPSTNGAGYPQLRDINDGTGEISIATSEYAKFAN
                     YPLPASTYRGDITVIVGWYRNYTNAAGAWQLTLRTINDLGKGFESYKAKIQK"
     gene            19368..20849
                     /locus_tag="PLM04_05080"
     CDS             19368..20849
                     /locus_tag="PLM04_05080"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HON02481.1"
                     /translation="MKKFNSKIVFTALAVLFTASCTNVEDVYIEKPTSDVIYSETFQN
                     GLGDFKAVNVFGDQVWTYNSSGYAIMTGYVNPTNYANEDWLISPEIDLTNIASAYFSF
                     DYVMRYSNDPANDATVWVLADHAYSPDTLPNVSQWVKLDLKPFVDPGSWTFSNSNDIS
                     LNDFVGKKIRIAFRYISSATKAGTWEIKNFTVKKGTAKVVESNDGSEEKPFTVKEAIQ
                     NQAGASAWVKGYIVGYIWSGTQNNYVFSADTCTQATNIILADTNDLSALYVSKILAVQ
                     LPAGVVRDSLNLKDHKDKFGKNVTLYGSLTKYFGIGGLKSVSYYKLEDGTSGGTKPVK
                     AIFSETFANSKGDFTIVDVTKVSVLPNIWAHDASYKCMKATAFDNPTNYESESWLISP
                     TINLSGVTNPVMVFDYVTRYFANPATDATIQVSENYQGGDPRSTTWTQIPYPFTNMSS
                     WTFVTTNPIDLSAFANKNIRIAFKYTSTSAKAGTWEIKNLVIY"
BASE COUNT         6872 a         3957 c         3776 g         6388 t
ORIGIN      
        1 tgaaagagtt cgacaaagtc aatcagaatg aattttattc taataacctg ctaacagtaa
       61 atttatgtca ttcgatttta tgttgtagta gtcgccaatt aacctgttta caaggaatcc
      121 cttataaaca taaacgccat gacgaaatcc tgaactttct gctatggcag cattgataca
      181 gccggagtga ccaattttca acaaaatggg agacagaata ttgctgagcg ccatagagga
      241 tgttcgggcc actcgggcag aaatattggg aatgcaataa tgaattacgc catgcttctc
      301 gtaaatcgga ttctgcaggg tacgacattc ggaagtttca aaacatccgc cctgatccat
      361 gcttaagtca acaataattg ctccacgttt catcgttttt accaaatctt ccgaaaccat
      421 aaatctttcg gaaccattga tatatctcaa atttcctatt accgcatcag ctgttgcaag
      481 agctttgaaa agtacggccg gatgaatgac agaagtaaaa acctgttgtc ccaaatgatg
      541 ttggatggtt cgcagtttat tgatatcgtg gtcaaaaatt ttgacttgtg ccccaagtgc
      601 caaagctgtt cgtgcagcaa cggtaccggt gatgcttgcc cccaaaataa ccacttccgg
      661 aggactgatg ccggcaacac ttcccagcaa caagcctttt cccccatgtt ggttgctcat
      721 tagttcggct ataagtgtga tggctgtgtt tccttcaatt tccgaaattg aactgaccac
      781 cggaaatccc ttttgttcgt cgcgaatcag ttcataagca attgcattga gctttttgct
      841 catcattgcc ttaatgcttt cggtagataa attactaagt tgcagcatgg aaaatatgga
      901 tgcatgatca ttcatgcaat ttatttcatc caatgtgggt ggtgtaattt ttatgacgat
      961 atcagcactg aaagcatctt gtggcgaatc gaccagaaaa gctcctgctt cgctgtattg
     1021 tagatcggag taggacatgg gttcgcctgc acctcgctcc acataaacgc tatgaccttc
     1081 ttccgtaaca atggcaacgc cttcgggagt aagtgccagc cgtgtttcga tggaggcatt
     1141 ttctttggga attccaatgg acaatcgtgg ttgattggca atttccttca acaaacattc
     1201 ttcgggaaat aaagaacgct gtttatgcag ttggtcgttt aaactcattt tcagtatgtt
     1261 tttgcaatat tttacaaaac taaatactat tcttgaaatg agcaaaaatt gtatctaaat
     1321 taatgctatt attgttgaat taattttttt atttttgttt gatattttgt taaatatttg
     1381 attgtcaatt gattttccac aaaaaatatt ttttcggtat tatctttcgg atattcacct
     1441 gccagatgca aaacaatgcg acagggagct ttttcgttga aaagttcatt gggaatggct
     1501 aatttccatt cagggctttt ggaagtgagt atcaccggct cattgtattc ggaggaaaat
     1561 ctcagtatat tctcggaaat cagtatattc tcggaaaata gtttttaaaa tttttatttt
     1621 agtacatgac aaaaattgag acatttgttt tatatatctg taaaacagga catataagtc
     1681 aagctcttgg ggttgccttg tcctgaatat atttgagtaa atcaacattt aaaaatctta
     1741 tgaatatcat taaattcagt tcacaattct ccaacaaaaa gttatttatt gtacattcta
     1801 aggattaaag atatgctttc ggaataattt attccaaatg cagcattgta aactattacc
     1861 cgatgcaaaa taaactctgt taggaataca aaccatgtcc tcattgtcaa ccctttcgtt
     1921 cttaaacatt aatggaaggc agtaaattac cttttagata ccgatatttt aactttgata
     1981 acattaaaaa gtatacacct tcttttttat gtacaatttg ttttatacat ttgtatgttt
     2041 tgtattttca attgaatttt ccaataacat tatgaaaccc acatgtattg catacataaa
     2101 cacgaatgaa tataatattc agacatgtgg gtttcatcag acctttaaaa aataaattat
     2161 attctgatga aatctattca actctcaaca gtatttttct tgtcgataat ttttctggta
     2221 gcatgcaata gcagggggaa gcaagaaaaa gaagccgata accaacttca gaaaatccaa
     2281 caactcattc agcaaaatga attaaatgct gcaaaaattg ccatcgacag ttttcacgca
     2341 aattttccgc gtttggttgc caaaagacga atagccgaag cttttcagga tactattaca
     2401 cgcagagaat gcaaaagaac aatagcatat tgcgacagta ttttgccttt taaacagaag
     2461 gaagccgatt cactgctgct aaattttcgg ttggaaaaaa acgaaaaata tcaggacatt
     2521 ggaaattacg tttacaaaac ccaatcgact gaagcaaatg cagctcgtac ttatttgaaa
     2581 gcatatgtag atgaaaatgc cgattattat ttggtgagtc agtatatggg accaaaaatt
     2641 gagcatactt cggttgaaat atccaacggc gaagtttttg cacataccga caccatcgac
     2701 attaccagtg cttcttattt gagcttttcg gatgaaaccg gacgttggga aattgtaact
     2761 ttcaaaaacg aagctgaaaa tggggtttca gaatttattt cgcaatatat gaatgaaaat
     2821 ctgaaagtta tcctacacgg aaaaaagaat ttcagctact ttcttcagaa caacgataaa
     2881 aaagccatta aggaaactta tcatttatgg atagtaaaaa aagatattaa ccgcttaaaa
     2941 aaggaaattg agaaagcaaa actgaaaata gagcaaatta acaggaggaa tccggtttag
     3001 gcgggaaaat caaatttctt ttttcaaata catttccacc agccgcgaaa cagcataatc
     3061 gccaatttcc acgtttggaa tttcgataaa ttgatttaat gcctcatttt tttcggcaat
     3121 ctgtaaagta atgaactgag ccgaaatgcg ccgatgcgtc aaaaggtgat gaacaggttt
     3181 tgaaatgttt ataattttta cttgttccag tccctgaaac aactggcaaa aaaactcatt
     3241 ttcaagcaat tcctgaacct gcaacaaatg gtctgtttcg atcaacggaa attcgtacaa
     3301 atttttccaa atatcgtccg ctgttctttt ctgcaaaaaa atatgatggt tataattgat
     3361 aaccagataa ttaaaaaatc gttgtttgat aggtgttttt ttagatttta ccggcaaccg
     3421 ctctaccatt ttcatctcat aagctttgca ataaacccgt agaggacaaa ccgaacaatc
     3481 gggagaaacg ggcacacact gcaatgcacc aaattccata atggcctgat tgtgaagaga
     3541 aggaacagaa ttttccatca attgctgagc aagagcatca aattcctttt tacctgaatg
     3601 gctgtcgata gacgtttcga tagcaaaaag gcgtgaaaga acgcgataaa catttccatc
     3661 caccacagca tacggaagat tgaaggcaaa agaacaaaca gccgctgcgg tataagctcc
     3721 aacacctttt aattgcataa cttccgaata agtggttgga aaaacgccgc caaaatcctg
     3781 catgatttgt cgcgcagcgt gatgcaaatt gcgggcacgc gaataataac ccaaaccttg
     3841 ccagtatttc aagacttcgt cttcttcagc ggcagccaac gtttcaacat ccgggaatcg
     3901 ttcgacaaac cgtaaataat aatccaatcc ctgattgaca cgcgtttgct gcaggatgat
     3961 ttcggaaatc catattcgat aaggatcaga aatgtttcgc cagggcaaat cacgtttatg
     4021 aacctgatac cagctgataa gttgatcgga taaattcata ttttcaaaaa attcaaagaa
     4081 aaactttcaa ctctgcaaaa aaaacaaaaa atagcctcga ttgaaatgta ctttatatca
     4141 aatgatattt acgaggtttt gccccaaacc tcactataag aaaaatggac gttcattaac
     4201 aaaaaatgaa ttgtcctgtc ggatttgcaa tccgacagga ttatttgcga attttaaatc
     4261 cgctattaaa acaccaaagg attacaaatt ttttttgacg aagacatttc atgaaacatc
     4321 gctaaaaaaa ctacaaacta gtaattatca attaaataat ccataatcag gactccaaac
     4381 tcggaactcg aaacttgaaa ctggtcattt cacttctgcc ccattcacta ctgttgtttg
     4441 aggttggatc aaagttattc tgccatcagc atcttcggcc gagagaagca ttccttcgct
     4501 gacaattcct tttaattttc gtggttcaag gttggcaata aagcaaactt gctgtccaat
     4561 taacttttca ggttcaggat aatactgagc gattccggaa acgatagtac gaccgcccaa
     4621 accatcatca atcttgaact gtagcagttt gtcggctttc ggaacttttt gacaagccaa
     4681 cactgtgccg acgcgaatat cgagtttttc aaagttttcg tacgaaatgt tggattttac
     4741 gtctttaggt ttaaacgccg aaagttcgtt ctttttcttt gtatttagca gtttctgaac
     4801 ctgatattcg atagcactgt cttcaatttt gtcaaaaaga agcaccggct ttttcaattg
     4861 aagctgaggt ttcagtaact caatgctgcc caaattattc cagtcgaaag tttccaaatt
     4921 caacaaatcc cttaattttt ccgaactgaa tggcaaaaac ggttcaaaga cgatggaaag
     4981 attggctgca atctgcaaac tcaaattcaa aatagtagca actctatcct tatctgtttt
     5041 tacaattttc cagggttctg tgtcagccag atatttatta ccaagccggg caagattcat
     5101 ggcttctttt tgtgcatcgc gaaatttgaa attatcgagc aatttttcga catttccctt
     5161 tatatcagag agttcgttaa ttgtctgtaa atcaatttct tgcaattcat ttcgaaccgg
     5221 aacttttcca tcaaaataat tttgtgtcaa cacaagagtt cgattcacaa aattaccata
     5281 aatagctacc aattcattgt tgtttcgagc ctgaaagtct ttccatgtga agtcgttgtc
     5341 tttcgtttcg ggagcgttgg cagtcagcac ataacgcaaa gtatcctgtt tatcgggaaa
     5401 atcttccaga tattcgttca accaaactgc ccaattgcgt gaagttgaaa ttttgtcgcc
     5461 ttcaaggttt aaaaattcgt tggccggtac attatccggt aaaatataag ttccttcgag
     5521 ttttaaaatt gttgggaaaa taatacaatg aaaaacgata ttgtcttttc caataaaatg
     5581 aattaatcgg gtatcttcac ttttccacca tttttcccag ctttcgggca atagttcttt
     5641 ggtgttggaa atataaccaa tgggagcatc aaaccataca tatagtactt ttccttcggc
     5701 cctttcgagt ggaacgggaa taccccaatc caaatcgcga cttacggcac gcggttgtaa
     5761 gcccatatcc agccaacttt tgcattgtcc gtaaacattt ggtttccatt ctttgtgctc
     5821 ttccagaatc caatgccgaa gccattcttc atgctgatcg agaggcaaat accaatgttt
     5881 tgtttcacgc agcacaggaa tatttcccgt aattgctgag cgcggattaa ttaaatcagt
     5941 aggattgagc gaggaaccgc aagcttcaca ctgatcaccg taagcatttt cgtttccgca
     6001 atgcggacat tttcccacaa tatatcggtc ggcaagaaat tgaccggctt cttcatcgta
     6061 atactgttca ctcgtttttt ctatgaaacc acctttattg gcaatggttt taaaaatatc
     6121 cgaagccaat tcatgatgaa tttttgaagt ggtacgagaa taaatatcga aagaaattcc
     6181 aaaatcttca aatgattttt taatcagaaa atggtaacga tccacaattt cctgcggaga
     6241 aatgccttct ttttttgcct gtatggtaat gggaacacca tgttcatcgg aaccgccaat
     6301 aaaaatcact tcttctcctt tcaaacggag ataccttaca taaatatcag ccggaacata
     6361 tacacctgcc aaatggccga tatgtaccgg gccattggca taaggcaaag ccgaagtaac
     6421 cagcgtacgt ttaaattttt tcatttaaat caattaaaaa tttgtcttga agagctttaa
     6481 aagaatgcgc aaaattatat gaaaatggct ttatttccaa acgcatgaaa ataaaaatca
     6541 aggtcgcaaa actttaaagg cttaccgtag cgtgctttcg agcacaaatg ttacctttgc
     6601 aagttggttt ttttaataac caatgaaata aactaatatg attattcgca atgattccta
     6661 ttgtaaattc ttgagttgaa ataggagtta taacataaat aaaaacatat atataattga
     6721 taattaaata tttaacaata aaaaacattt ggtatatatt aaattttttc aaatccgctt
     6781 ttcgaattgc atcaaataat tttcttttga aagaataaaa atgtaaattt gaaaaatctt
     6841 tttttaaatt ttaataacca tgaatatgca actttcaatt ttgaaaaatc tccgtttttc
     6901 aggagtattt ttaataagta ttttttctgt atttatttca tactcggctc ctcgttatga
     6961 tttgattcct tatccacaag aactaaaacc tcaatctggt gaatttattt taaataagaa
     7021 aaccgtcatt ctttgtcctt ttgatcagcc ccaaatcgtt gaaattgctc aacaattttc
     7081 tgatcatttt gctctggttg ctggcataaa attaccttta cgggattatc aaacgtcttc
     7141 tacaaataaa aatttcattt tatttaatac aggttcatta acaaatgaaa atcctgaagc
     7201 ttacacctta aaaatttcta cttcttcggt tcaaattaca gggaacacag cggctggcgt
     7261 tttttacggc ttacaaacat tatatcagct tttcccgtgc gaagtttatg cttcaaaaga
     7321 aagcgaaaaa ataaaaaaat ggtcagttcc ctgtgttgaa attacagatt ttccgcgttt
     7381 cagttacaga ggacttcatc tcgatgtttc tcgtcatttc tttcctgtag attttataaa
     7441 aaaatatatc gacttaatgg ctatttataa gttcaactat tttcattggc atcttactga
     7501 tgatcagggt tggcgaatag aaataaaaaa atatcctcga cttacagaaa ttggttctaa
     7561 aagaaatgaa acattgattg ggcattatta tgagtcgttt ccacaacaat ttgatggaaa
     7621 accttatggc ggttattata cccaagcaga agctaaagaa attgttgctt atgctaaaaa
     7681 acgaggcatt actgtaattc ctgaaataga aatgccgggg catgctcaag ctgctattgc
     7741 tgcttatcct tttttgtcgt gtacccaaga cacaactatt aaagttgcca ctaaatgggg
     7801 tatatttaaa gatgtatttt gtacacgtga taccgttttt aattttttag aagatgtatt
     7861 atctgaaatt atggagattt ttccttcaaa atatattcac attggaggag atgaatgtcc
     7921 gaaagataga tggaaggaat gttcctattg tcaatcgttg ataaaaaagt tgaatttgaa
     7981 agatgagcat caattacaaa gttattttac tcaacggatt gaaaaattta ttaatgaaca
     8041 tggtcgacaa atcattggtt gggatgaaat tttagaaggt ggtttagctc ctaatgctac
     8101 agttatgtca tggcgaggaa aaattggtgg cattgaagct gctcgtcttt cgcacgatgt
     8161 gattatgaca ccgacggaat actgttattt agattattat caagctgatc ctgaatttga
     8221 accactcgct attggtggtt ttgttccttt atccaaagta tataattttg aacctattcc
     8281 agatgagttg actcccgcag aagcaaaata tatattggga gctcaagcca atttgtggac
     8341 agaatacatc cttgatgaaa aacaggtgga atatatggct tatccacgtg caactgcatt
     8401 atcagaagta gtatggagca acaaggaatc aaaaaattgg gaaaatttca aagaacgttt
     8461 gtcaactcat tttcaacgat ttgaaaaact tggagtgaat gcctctcatg cattttatga
     8521 acctcaattt aatgctcaag taacggaaaa taataaatta aaaattactt taagttgtga
     8581 tcatccaaaa gcaaaaattt atttttcatt aaatgaaaaa aaccccgatc agcattactc
     8641 aaaaccgtta ataattgatt caactactat tgtatcagtt gctacttttg ttgatggtaa
     8701 gaaagcaagc agaactttac aaaaaacttt catagttagc aaattaacaa atttaatttg
     8761 tcaacctgac tccaatgttt cttatcatca acaaagtatt tttaaattaa ccgatggaaa
     8821 attaggaaat ataaagacca cttctgaatg ggcgagattg atatcaaatg aagattattt
     8881 attaaatatc gatttacaaa agaaccaaat tattcaacgt tttacgatgg gaatattgaa
     8941 agcacctgct ttttgtggac aaattccttc tgaaattatt ctttatgggt caaaagatgg
     9001 aattaattat caacctcttg aagagcaaaa attctcatct tcttttactg gaaaatatga
     9061 aattatacga ccagtattta attttcctcc agttgaggtt cgttatttaa aaatagtgtt
     9121 gaaaaatcct cgtttttgta ttgaaaatct tgaaaatgaa aaaaataatt ctattttcat
     9181 agatgagatt gctgtatggt aaacagttta tcagagaaat ttttctgtaa ttgttctgaa
     9241 tcactgttga cctacaaaaa aaattaagta aaatggaagg gacaagttgg aagcctcgtc
     9301 tggggtagaa aatttgtatt tcaaactcaa attaacagtc ctggttaaag atacaacgac
     9361 aaattttgta ggtcagccga aaagaaaaag tcagtttttg agaaatttta agcattcgat
     9421 tctactacta tcatactttt tcccgatata atgaaacgga taggcgggaa tcccaaaggt
     9481 gaagggaaaa ggaaaggagg gttgaaagta cacatgctga ccgatgacat agtcagacgc
     9541 cttttttagt gaaaattagt aacgcaaaga tgcacgacaa gaagtttttg acatttttgc
     9601 atccttgatg ggaagtacat tagtttacga taaagcctat aattactatc tccaatttgc
     9661 tgcctggaga gagaaagaag taaattttgt ttgtcgtctg aaagaccatg tcaagaaaca
     9721 agtacaagag gttttgttta agaaaaagtg ttcaaaagaa gcatttggtg tttacagggt
     9781 agggcatata cacttggatt ataaggaaaa caaagaaaga aaaaaacact ttgtttgcgt
     9841 ttggttataa taaagatgag aaagggcaaa aatacaagtt ataaccaata attgagaaat
     9901 aagagctgaa gaagaaactt tgaaatacca ttatcgttgg acaatagagt tgacatttaa
     9961 aaaaactgaa acaaaatctt caattgcatt ttttctattc tgagacaaaa aatgtgatgt
    10021 aaaatatcaa aacaacaaaa aatttaatgc ctcttttcaa aaaaacctga ttttttctcc
    10081 cttttgaaaa aatcaggttt taagagggga ttcaaaattg aaacaaataa aaaatacaat
    10141 tagttaatta tcaattattt ttttaaaaaa tcttgtctta tactgaatta aacaccaaat
    10201 atttctttaa tggatttttg aaaggtcgta gggtgtttcc tgataaacga aataattcaa
    10261 ccagttagta aataaaagat tggcatgact tctccatcga accagcggtt cattggcagg
    10321 attattgtcg cgatagtagt tttttggcat atcaataggc aaaccttttg ccaaatctct
    10381 tttgtattca ttatccagtg tggtagctga gtattccgaa tgtcccgtaa tgaaaaactc
    10441 tcttccgttg cgtcccatta ccatataaac acccgattcg tccgattcgg aaagcaatat
    10501 cagttcagga ttttttttga tatcctccgc cctgacttcg gtataacggc tgtggggcac
    10561 ataaaaaatg tcgtcgaacc cgcgaaaaat gggattttgc ggattatgaa tataatgctc
    10621 gaaaacgcca aacattttct tttccaacgg atattttgga ataccataaa aatgatacaa
    10681 tccggcttgg gctgcccagc aaataaacat ggttgaagtt acatgcttgc gcgaccagtc
    10741 aaatatctct tgaacttcag gccaataagt aacttcttcg tattcgagct gttctaccgg
    10801 agcccccgta ataatcatcc catcgtaatt acttgcttca atggcatcaa aagttttgta
    10861 aaaagccatc atgtgttcaa taggcgtatt cttcggagtg tgtgatttca attgaagaaa
    10921 atctatttca atctgcaggg gagaattgga aagcaagcga atcaaatcgg tttcggttgt
    10981 aattttgagc ggcatcaggt tcagaattac aattttcagt ggcctgattt cttggctcga
    11041 tgccctttcg gaatccatca caaaaatatt ttcctgcctt aatatttcaa cagccggcat
    11101 cgattttggt atatttaacg gcatagtcgt gaaaagtttt tatattctgt tttaatttga
    11161 atcaaataaa tagctgaaaa gcaagctctg tccattaaaa ttttattcag taatactttc
    11221 ggtagatatt ccaataggcc gaacttccaa taaaatcttt aagaaaatcg ttcaattgct
    11281 gaagcagcaa tttcgattcc ttatccacca tccatgcata atgctgatcg aacccaagcg
    11341 gcaacgaaat atcaatctgc ggataggtta atttcagaaa ccgggcttct ctttcatcgc
    11401 aaactgtttg ccttattttt ccctgcgcaa cccattcgac caactgctcc aaattaacat
    11461 taggaacttc gacaatatga atggtatcgg ctatttcgtc cgaaagatgt ttcaatcgca
    11521 acttgtgcgg cgacattttc ggaacaaata tcgtatcgcc ggccaaatct tgctgagaat
    11581 ttttcatcaa tcgtttcaaa gaatccggaa aatttttttg taccaaaacc aaacgggaag
    11641 tattgagcgg atcggaaaaa gcaatatcgt tcatccattc ggtggttacg ggaataaaac
    11701 cggcgtaaat atcgtaatcg ccatttgtca atcctttcag acaagtttca aaattatttt
    11761 caggggtaat aaccagtgtc aaacccaact gatcggcaaa agttttaact atttcgtact
    11821 gaaatccgta aacgctgtcg ccttccagac gaaaacccat ccggctactg tcggtaacaa
    11881 cataaagttt ccccgatttc agcatggcag gcaaatcgtg cacgggcttc tcatgaaaaa
    11941 cagcccatgc gccagccatc aagagcagca ctgccacaaa ataaattgtt attctaaaac
    12001 cctttctcat gatttaaaaa atgttgaact acaaatctat cattttgttt acagcaaaat
    12061 caattgtaaa aattggtagc cacgccaatt ttaaatatgg cagggttaat gggataattc
    12121 ggcatagaga aataggcatg attcataaac aattgattca catggaaata ttctacaaag
    12181 aaacgagcct gtttcaaatg aaagtttgca tacacattta ccactggaaa attcccgact
    12241 ttggtttccg attgcgtata gaattgagct atggcaggca tgtagcaggg agcataatag
    12301 gcagtatggt aacgaacatt tgcacctaat tgaatactca aaacattaaa caacatgttt
    12361 ttaaagtaaa gattatgaaa cagggtcaaa gtcggcaaag gtaaaacacg ttcttgcgaa
    12421 ctcaattgat acaccacatt gttttccaaa acaaaatttc caacatgaaa atcctttttc
    12481 aaattggctg caattacctg aatatttcca tcatattgct ccggcaatgc ttcggaattg
    12541 aaatatacat aattggtcag attttctacc gaaacattca ctgaaaattt tcgagtgggg
    12601 atggaaaaag tgccaccggc attggttcga tatattttgt caaaattgtt ttcccaccgg
    12661 aaatggttcg attgataata atttaaaaaa taagaaggtt catcgctgcg aacaaaaccg
    12721 ttagccgaaa gcacaatact gtctttccat gctttgaaaa atccgtttac ctgtcccttt
    12781 atcgaaaaat cgccctgttt atacccgagc aaataaatat cgcccaatat gttgtatcta
    12841 aatctttcgc ctcgttgttt ggacaaaata cctcccactt ttgtgttcga ttcgtttttc
    12901 ttttggatca gcgaatcgac attgtaggta aatcgttgca attcattttc tacaaaacct
    12961 gtcagtccaa aacgcatcca tttattgaac tcttcggcaa ttcctaccga cacagtattg
    13021 gtcaaagttt gcaaagctgc cgtatcattg gtttgtgtaa aaggcaaata agtattttta
    13081 taaaaatctt tttcgacatt cgcttcataa tagcgttttc gcgggtcttc atattttatg
    13141 gtatgcccga aatgggtaac cggcacgtat tctttcctca cggaatcggg tgagattctt
    13201 attatctggt caaaacctat agtatatttc tgatgataaa agaaaacatt ttttcgataa
    13261 gtggaatatc catgtatatt gacggggata tcctgcgttt ttacgccaat cggttcataa
    13321 atataggaag agtcgctaat acctccattt tcatgattga acatattatt gaccgcagcc
    13381 aaaccaaccg cgctgtaatg cttgccatcg taactaccaa acagagaacc cgtaaaacgt
    13441 ttggcagcct gattggcgta ttctcccacc gaatagatgt aatccagcgt cgtgccgaaa
    13501 ttcaatcgct tgttggcatt aaaggtaaac aaaaatttaa tttgatcttc ctgacgataa
    13561 ttgcttccac cggtattgta aacaattttt gtgaaaggag tttttgtatt gtaaaatacg
    13621 gcactttgca tgtcggtaat ataaggataa aaagcatccg aaaaaataaa atcgttactt
    13681 tccggacgac tgaaatacag tttcgactga atgggcgacc ccaaatttcc attatacgaa
    13741 ttggcaatac tgaaccgatc gatcggattt tcatcctgaa agttcaaatg cagcgtatct
    13801 acctgtacag aatctgccat ccccaatgga tcggaaactt tccaagtttt aacataacgg
    13861 atcgatggcg gattgattgc ccaaaccgat aatgcagtaa ctataaataa aataaacaaa
    13921 aaataccttc tcacccgtac tgataaaaaa attgaaaagt caatttgttc ctaactcatt
    13981 tttagaaagg acaaaattaa gaaaaatatc ccgtaaatat attttttggc aaagtcttat
    14041 tgcaaaaaac cttacccaag caatactcaa gtaaggtttt tgttccgaaa aaagcagaaa
    14101 aagttagttc tcgttagcct gactgtctac ttctttaatc aaataaatca aggttggaaa
    14161 atggtcgcta aaaccattca gccacactcc tccggcaaat gtacgccacg gatttccctt
    14221 gtagcggcct tcctgctgaa tcagaaaatc acgattgaaa acttccgctt tccaaaattt
    14281 caattcggaa ggtggagcat tcagcaatgg agctgaaata atgatttgat caaacaaatt
    14341 ccactggtcg ttataggcca aggaaccaat tcccctatcc aaaattttcc agaaaggatt
    14401 gtacagttcg gtttctttca cttccgaagc attttttttg gctcccaaaa ccttagccac
    14461 actttcgtta aaggggtcat cattcaagtc gcccataaca attattttgg catttttatc
    14521 cacccgataa agagaatcga caatggagcg cgtcaaagca gcagcggcat tacgtttggg
    14581 gcgcgaacgc atttcgcctc ccgaacgcga tggccagtga ttcacaatga catgaatttt
    14641 ttctccaaaa agataaccgc ttaccattag ctgatcacgg gtacggaaag ttgtgtcagc
    14701 agtatgcaat cgataagatt tgctattgga aacaataaaa tatctcggat tatacaaaag
    14761 tgcaacatct acgccccgac ggtcaggacc atcataatgg acaatttcgt aagaacgttt
    14821 ttttaatgac ggttggctca ccaaatcttc taacactcct cgattttcaa tttcagaaac
    14881 accaatgatg gcagctccgt caggtgttac atccaacccg atttgagaaa tggcatacga
    14941 cattttctcg agcttcgatt tgtatttcat cgaattccaa cgatttgggc cattgggtgt
    15001 aaattcataa tcgttaacat tttcattgtc aatggtatca aataagtttt cctggttgta
    15061 gaaagctaca caggcaattt ttacgttgat ttttttctcg ttcgacgccc aaagattgag
    15121 tgatgaaaac agaaaaaagc caaaaattaa agcgtaaaaa gaaaaatact taaagaaatt
    15181 gttcatcttc atggcaaaaa aattattgag aatttatcgt tttattaaag agggacaaat
    15241 gtagtaaaaa agaaatacaa agaaaaaaat tgaaaaataa atttagaatt ttgtcaacaa
    15301 atctcacaaa ttactgtttt aattaaaatt aaaaatttca tttacaacta attaatcata
    15361 taaataaaga ataaacagta ttttaaccaa aaaaaaattc tacttcgctg ttttaattca
    15421 aaaaaagatg ctaacttgca cgcccttttc caaaaaacat taaaaacaga aaaaaacaaa
    15481 ttaacacttt ttttttgatt aaaaatttca tgttattcga agtcgttaat aacgaacaac
    15541 ttttatgcaa tccacaaaaa acaaattaaa tcacaactgt atgaaaaaac tatttctgat
    15601 actttttgta ttctttttgt gcattccact tttgatggca gaaacttcca tcaaaggaac
    15661 cgtaaagtcg gccgacacac aaaatccgat tcccggagta attgtttcct taatagggca
    15721 gaacatttcg acacaaacca atgcggaagg tcaattctct ctgaggttta tcgaagccgg
    15781 cgatcaggaa attcgtattt ccaaaatcgg ctattttact caaatcaagt taattcatat
    15841 tgaacccgac aaggaaaatg atttgggaac tttttatctg cgtgaagatg ttcagcaaca
    15901 aacaaaacag gaagttgttt tgcaattaag tgaaaatcag atagatgaag atgaaggtcg
    15961 tgctactcaa aatatatcgg ccgctttgtc atccaaaggt gatgtatatg tttctcaatc
    16021 cagcttttct tttagtccga tgcgcttcag aacaagagga tatgatgata attttgaatc
    16081 cacctatatc aacggtgtat attttaacag cttggaaaga gggggattca actattcttc
    16141 gctgggtggg ctgaatgatg ccatgcgaaa tcaggattac acctacggtt ttgctccaaa
    16201 ttccttcagt tatggcaatc tgggaggaac taccaatatc aatacaaaag ccagcaacta
    16261 cgcagccgga acaaaagcca gtgttgccta caccaatcgt tcctataaat tgagaggtca
    16321 ggctacttat gctaccggtt taatgcaaaa tggttgggca tttgctggtt cagctgttgt
    16381 tcgatgggca gacaaaggaa ttgttgacgg cactttttac aattcgtggg gatatttctt
    16441 ttctgccgaa aaaattctta acaaacaaca cagcctgtct ttcgtaactt ttggttctcc
    16501 aactcaacgt gcccaacagg gcgccgttac ccaggaagtc tttgatttgg ccggttcaat
    16561 ttattacaat ccgtattggg gttatcagga aggaaaaatt cgaaattcac gtatcgtaaa
    16621 aagtttcgac ccaaccgcta tcctttctca tgaatataaa atcagcaatc atcagttgtt
    16681 gcgaaccgga ttggcatttc attacagcct ttacagcaac tcggctttga atttctacaa
    16741 tgctcctgat ccacgtcccg attattatcg caatcttccg agctttgtga gtgatgattc
    16801 gttaaaaaat gtgattgcca atcaatggaa atctgacacc aatgtttctc aagtcaattg
    16861 gagtgctctc tatcaagcca attacaaaaa caatgtaatc aatcccaacg gaactgcacg
    16921 atatatcgta gaacgccgac acaacaacct gatggaagga gctttgaact caacctatac
    16981 ccagcaagtg aataaacagt tgaaacttat tgccggaata gaaggtaaaa tgtcgaaagg
    17041 aatgcattac aaaacacttg acgatttatt gggcggtaat caatggattg acatcgatca
    17101 gttttccgaa cgcgatttcc cttcaaatcc cgatattatt cagaatgatc ttagaaaccg
    17161 aaacgctgtt ataaaggaag gcgacacttt tggttataac tacgatatca atatggctta
    17221 tgccaatggt tttattcaaa atgaatggat acttccacag tttgatatct attatgccgc
    17281 taaactgacc tatacgcaat tctatcgcta cggacacatg caaaacggac gtgctcccga
    17341 aaattcgtac ggaaaaagca aaacttggta ttttgtcgat ccttcattta aagccggttt
    17401 tacttataaa attgacggac gcaatcgttt gtcattcaat ggactgatag aaagcagagc
    17461 gccgctcgcc agccaagctt atctttccga aagaataaag gatatgttga ttccttattt
    17521 gcaaagtcag gatattcggt cggccgatat taactacaat tttacctact cgaccattag
    17581 aggaagaatt ggcgtgtttc agacaaattt atacaacacc tccgatatgt tgggatatta
    17641 tgatgacgaa tacagaactt ttatcaacca tgtgttggca aaatcggaca aggtttataa
    17701 gggagtcgaa ttgggaacaa cggtaaaaat caacagtaat ttctccgttt ctttggcagg
    17761 aacgtacgcc gattatcatt atgccaacaa tgccatcggc gtaaaaagtc ctgaaaacgg
    17821 tgcttttgcc gatgttgccg aaaccgttct tacaaaaggt ttaaaaattg ctgcaggtcc
    17881 tcaagcagcc ggaaatatta ccatcgatta tttccatcct aaaatgtggt ttgccgatgt
    17941 taccttaaat tatttcgaca acaattattt ggattttgcg cccaaccgtt tcaccgaaaa
    18001 caacatggca aaatatacca cacccgaaat gatggctgct ttgggcaccc aggaaaaact
    18061 gaaaggtggt tttctgctgg atgcttctgt aggaaagctt atttatttga agcatcgtcg
    18121 ttctttgaac tttaacttaa gcgccagcaa catactgaac aataccaaaa tgattaccgg
    18181 tggttatcag caagcccgct tgccgctctc caatggagcc attgacccca ccggtctgaa
    18241 cagatttccc aataaatact attatgcttg gggctttaac gtgttcttca atataggata
    18301 caagttttaa tgaataaaaa tcatatattt atagatattt aaaatatgac gaaaatgaag
    18361 aattttaaaa taggtggaat ttccatcctt ctggcagcaa tttttacatc ttgtgccgaa
    18421 acagattttg atgcaattcc cgtcaacaag ttgatgggag attcattgac tactacttat
    18481 accattgaac aattgaagaa taattttttg actttaaacg atgcttattc cgatacagtc
    18541 aattataaag gtggcttata tacagctgat cccattcagg ccgaatcgga cattgtaatc
    18601 agtggttaca tcacttctac tgatgtggat ggaaatgttt acaagtattt tgttgttcag
    18661 gaacccggag ctaacggtca agccattaaa gtttcgattg atgcttcagg attatctgca
    18721 atttatccac tcggacagaa agtatggatt cgatgcaacg gtttgtacat aggacgttat
    18781 ggagaagccg aacaaattgg ttcacgctat gtaaacaagg aaagatttaa agttaaaaaa
    18841 tcaactggag ataccattta ccgaatagaa ccaggccgaa tgccattttt gattgccaac
    18901 gaacacattc acgcttacgg aatgcctgat cctacggtta ttaaacccga taccatgacc
    18961 attgctcaaa taaaagcatc aaatcatact aaacttgtca acaaactggt atgcatcaaa
    19021 aatgccttct tcacgggcaa aagtgaaggc gataatctta ccgatagcga actgattttt
    19081 gctccatcca ccaacggagc gggttatccg caattgcgcg acataaatga tggtaccgga
    19141 gaaatcagta tcgccaccag cgagtatgcc aagtttgcaa attatccgtt acctgcttcc
    19201 acttatcggg gtgatattac cgttatcgta ggttggtatc gtaattacac taatgcagca
    19261 ggcgcatggc agttaactct gagaaccatc aatgaccttg gaaaaggctt tgaaagttat
    19321 aaagcaaaaa ttcagaaata aatccgacat taaattacga aatcaaaatg aagaaattca
    19381 attcaaaaat agtttttacg gctttggctg tgttatttac ggcaagctgt accaatgttg
    19441 aagatgttta cattgaaaaa ccaacttcag atgtaatcta ttccgaaact tttcagaatg
    19501 gtcttggaga ttttaaagcg gtaaatgtat tcggcgatca ggtttggacc tacaacagca
    19561 gtggctatgc cattatgact ggatatgtca atcccaccaa ttatgccaac gaagactggc
    19621 ttatttctcc ggaaatagat ttgaccaaca tcgcttcagc ttatttttca tttgattatg
    19681 tgatgcgcta ttcaaatgat ccggcaaacg atgccactgt ctgggtattg gctgatcatg
    19741 catacagccc cgataccttg ccaaatgttt cgcaatgggt aaagctcgac ttaaaaccgt
    19801 ttgtcgatcc cggaagttgg actttcagca attccaacga tatcagtctg aatgattttg
    19861 tgggaaagaa aatcagaata gcattcaggt atatttcttc cgcaacaaaa gccggcactt
    19921 gggaaattaa aaatttcacg gtaaaaaaag gtactgctaa agtagttgaa agcaatgatg
    19981 gcagcgaaga aaaacctttt accgtgaaag aagccattca aaatcaggct ggagcttccg
    20041 catgggttaa agggtatatt gtgggatata tttggtcggg gactcaaaat aattatgttt
    20101 tcagcgctga tacctgtact caggccacca atatcatatt ggccgatacc aacgatttgt
    20161 cagctttgta cgtatcaaaa attttagctg ttcaattgcc tgccggtgtt gtgcgcgata
    20221 gtctgaacct gaaagatcac aaggacaaat ttgggaaaaa cgtaaccttg tatggttcgc
    20281 taactaaata ttttggaatt ggcggactga aatctgtttc ctactacaag cttgaagatg
    20341 gcaccagcgg tggtactaaa cccgttaagg ccatttttag cgaaacattt gcaaattcaa
    20401 aaggagattt cacaatagta gatgttacta aagtttctgt tctgcctaat atttgggctc
    20461 acgatgcttc atacaagtgt atgaaagcta ctgcttttga caaccctacc aattatgaat
    20521 ctgaaagctg gctcatttct ccgaccataa atctttcggg agtaactaat ccggtaatgg
    20581 tttttgatta tgtaaccaga tattttgcaa atcccgcaac agatgcaacc attcaagttt
    20641 ctgaaaatta tcagggaggc gatccaagaa gcacaacgtg gacacaaatt ccttatccat
    20701 ttaccaatat gtcgagctgg acatttgtaa caaccaatcc gatagattta tcggcttttg
    20761 ccaacaaaaa tattcggata gcgttcaaat atacttctac cagtgcgaaa gccggaactt
    20821 gggaaattaa aaatctggtg atttattaat acagttaata taagcggtta attgtttttt
    20881 atttttgaaa agctacagcg ataactatcc tgtagctttt ttggtatgtc gggcatgtta
    20941 ttaagcttgc aggatgcgag ttccggtatg tgccgagttg ataggaaaca cta
//
LOCUS       DAOTAX010000034        20793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2092_length_20793_cov_30.549330, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000034 DAOTAX010000000
VERSION     DAOTAX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20793
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_20793_cov_30.549330"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PLM04_05085"
     CDS             <1..1229
                     /locus_tag="PLM04_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON02482.1"
                     /translation="NRFPVLKNVVFIGQEKHRGMYTTAEILLLGTYQNNEEYFQLKKT
                     INCHDVVNMQYTSGTTGFPKGVMLTHHNITNNGFYTGEHMKFTNDDKLCICVPLFHCF
                     GIVLAVMNCLTHGCTQVMVEKFDALLTLASVHKERCTALYGVPTMFIAELNHPMFNLF
                     DLTSLRTGIMAGALCPIELMRNVNEKMYMNITSVYGLTETSPGMTQTRVDDSFEVRCT
                     TVGREYEFVEVAVLDPKTGAPCPDGVEGEMCCKGYNVMKGYYKNPEATHQVIDANGFL
                     HSGDLGVRDKNGNYRITGRIKDIIIRGGENISPIEVEDFLYQMPGIKDVQVVAIASPR
                     YGEDVAAFIIKKDGFDIREEDVRDFCKDKIARYKIPRYVFFVDEYPLTGSGKIQKFKL
                     REMALQICKERGIEIV"
     gene            1288..2067
                     /locus_tag="PLM04_05090"
     CDS             1288..2067
                     /locus_tag="PLM04_05090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON02483.1"
                     /translation="MKKLWRIYRYFLHRFTARNTHGFGVHSPFLFHFICFVVEEKNPY
                     YIFSSIENLRDRLLKDRTKIQIEDFGTGKSRTSTIATVVNKSVKSKKYGQLFFRITLH
                     TKAQSVLELGTSLGITTAYLAAASSQLHCISLEGSQTLAALAKKNFQQLGLKNISLIE
                     GNIDENLEKVLESTPSFDLIFIDANHTSQAVIEYFNMCLEKIHTHSVMLVDDIYWSAD
                     MLKAWKYIKQHPKVTATIDLFETGIVFFDPNLYKKHYKVRY"
     gene            2217..2777
                     /locus_tag="PLM04_05095"
     CDS             2217..2777
                     /locus_tag="PLM04_05095"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HON02484.1"
                     /translation="MKLYTKTGDEGETGLIGGTRVPKNHIRLEAYGTVDELNSFIGLL
                     TTFQIDNSDLLFLQSIQNKLFTIGSYLATDFSKFSKTKVSPLFEKDITSIEKEIDRLT
                     SEVPALKNFVLPGGSQASALCHICRTITRRLERRLYDLHEQIPVDKNILIYINRMSDY
                     FFALSRYLLFKNNIQEILWQKNEEND"
     gene            2865..3086
                     /locus_tag="PLM04_05100"
     CDS             2865..3086
                     /locus_tag="PLM04_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HON02485.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYESIEDIWPDYPSKEDFFFNEEEY"
     gene            3269..4216
                     /locus_tag="PLM04_05105"
     CDS             3269..4216
                     /locus_tag="PLM04_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HON02486.1"
                     /translation="MKKIVLTVVVFMVSSVSIISQTASEWSQYMFNSSSLNPAAVGEK
                     GMIEAVWQHRIQWVGMPNAGQSDIINLNLPVDFLSTTHGVGLKVIDDKVGLFSNTSVY
                     LQYAYKLKTTSGVWSFGTELGLINAGFEGDSVHSVNIGDYHESTDPVVPSTNVQGMGF
                     DIGLGAWYSSPKFEAGISYAHLNQPVISWSDQLDYQEKGVLFITGGYNWTLIDPKMVI
                     KPTTLFKTDFVSYQWDASARMEYDNKYWGGLSYRWGDAVVFFAGINAIAGLNIGYAYD
                     LPVSQIIKASSGSHEFLISYSFEYKLNKAKHKYKSIRIL"
     gene            4286..5641
                     /locus_tag="PLM04_05110"
     CDS             4286..5641
                     /locus_tag="PLM04_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HON02487.1"
                     /translation="MLISILFGSLLLAGCNKPAGELIGVGNRGNFREANPYGMVFIKG
                     GSFLMGANDQSAIGAINDKSIHVTVDAFWMDQTEITNDEYKQFVNWVRDSIILRSLVM
                     AGRDEFRLKLRNQDELPPETAPLNWKAKIPWKSQDEEIREVLNTFYYEDVNSLSGRQL
                     NPTKFRYRYEWINYDQAALASNKYDVNTGAYPKNAKVRVDTSYVDENGVIHHQTIERP
                     LRTRKDLISTKIVNIYPDTIMWIRDFQYAYNEPKMKMYFSHPGFANYPVVGVTWEQAQ
                     AFCQWRTHIYNGTNKISAQDYRLPTEAEWEYAARGGRQMALYPWGGNYVRDKRGCFMA
                     NFKPMRGSYNDDTGTTTMKVGSFLPNDFGLYDMAGNVAEWTSSAFNVSSSNIVYDINP
                     NFQFNAKNSDPQVLKRKVVKGGSWKDIAYYLQCGARTYEYQNESRPYIGFRCVRSYNG
                     E"
     gene            5668..6546
                     /gene="gldL"
                     /locus_tag="PLM04_05115"
     CDS             5668..6546
                     /gene="gldL"
                     /locus_tag="PLM04_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HON02488.1"
                     /translation="MSNNAINSKKQSGFSEWWNSPKGRKIVNAIYSVGAGVVIIGAMF
                     KILHLPGANVVLGTGMICEAIIFSMGALDKPFKEYHWDKIFDFENGTARFQTELIGSA
                     TISASPAHPSINYSETLSEEDVEKLSEGIKNLSATARQLASVSDLTAPTSEFIRNITS
                     ASESVSNFSKDQNNLNSSLRNLSGIYVEIGTEMKKAEENTKDYTGEIEKINKNLSSLN
                     AIYEIHLKNVEEQSTKIGNSNKELENILSNLKKINSFSSASAEESEKYKNETVKLAQQ
                     ISDLNKVYGNMLNALS"
     gene            6609..8144
                     /gene="gldM"
                     /locus_tag="PLM04_05120"
     CDS             6609..8144
                     /gene="gldM"
                     /locus_tag="PLM04_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HON02489.1"
                     /translation="MSGAKNCPETTRQKMIGMMYLVLTAMLALNVSSEVLEGFTMVDN
                     SLHNTIISASVRNEDLYKQFEYLYNQNPTKVKEWLDKALDVKKQSDSLYNYIQNFKYE
                     ILKMVDNKNADPEARRVINKENYDAPGEYALVKGNGKILKEKIDNYRNFLISLSGNSP
                     AKQQMYATIFSTAPSENGVPWEYALFEMMPVSAVITVLTKYQSDIRMAEAELIQYLKA
                     QTDVSDFRVNKIEALVVPNSRYVFRGSKYSAQIVLSAVDSTKTPEFYVGNTPVKNGIY
                     EVNCNQTGSFTYTGFVQLIGNDGVIRKFPFTSDYIVGEPSATISNEDLNVVYRGIDHK
                     FSISVPGVASENISVRVAGATVQKTAGGKFIIRPTQDGEINVAVYAKVEGKEILMGNS
                     IYRVRYLPDPKAYLQYSDAGGITRLIQDGNLNKRLLRGDNVAIVASYGEDELIQAKFN
                     IISFTMLTIYGSVETTGSRLSNRQLADIDRLEGGDYITFKNIKAVGPDGKVRNLGLVQ
                     VQI"
     gene            8194..9096
                     /gene="gldN"
                     /locus_tag="PLM04_05125"
     CDS             8194..9096
                     /gene="gldN"
                     /locus_tag="PLM04_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HON02490.1"
                     /translation="MKKIGVLTLFLAFILLPGSIKAQINEIPFFDSKGNVNIETTELD
                     TLADTIAVVHHRRDDIVWSRVVYRVIDMREKQNYQLYFPTRPNKEYRNLFTVMIDAIC
                     DGVNVYKKNPRDIKPEFNELLEGEELSRAFAFEEDNTSNLIQIDPITNQRTFNNYRYI
                     QYVRNQLKYLIQEIIFFDKHTSRMYSKIIGIAPLYALHPDNVESNETMGYFRGSVLCW
                     FAFDELRPYLAKQYVIPNGNDTQRMTFDDFFSQKLYASYLLGDSNMFNRMLLDYVIDP
                     AKVRKEQNRIENELLNFEQDLWEY"
     gene            9303..9953
                     /locus_tag="PLM04_05130"
     CDS             9303..9953
                     /locus_tag="PLM04_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON02491.1"
                     /translation="MKNIILSDNQYISKAGWLYLISTYSLEATIREAATKKELEKLLV
                     EEQESLVILDYTLFDFENVNELIIWSMRFEKTEWLLFSEELSEEFLQQIIYNTHSFSV
                     LTKNSNKEEINLALKETLSGNRFICNFVSNLLIDCQKHLKNDGKPVLTPTEREILKEI
                     ALGKTTKEIASERFVSVHTIMTHRKNIFRKIKVNNVHEATKYAMRAGIVNLAEYYI"
     gene            9968..11029
                     /gene="mnmA"
                     /locus_tag="PLM04_05135"
     CDS             9968..11029
                     /gene="mnmA"
                     /locus_tag="PLM04_05135"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HON02492.1"
                     /translation="MKIAALLSGGVDSSVVVHLLKEQGYTPSLFYIKIGRDEDEMLNC
                     SSEEDIEMASLVAQKYDCKLEIIDLHKDYWETVVAYTISKVKEGLTPNPDVMCNKLIK
                     FGIFEQKVGKEFDKTATGHYATITEKDGKIFLSTAKDPIKDQTDFLAQIDYLQVSKLM
                     FPIGHFMKSEVRSIAQKAGLPSAKRQDSQGICFLGKINYNDFIRRYLGEKPGPIVEWE
                     TGKILGKHKGYWFHTIGQRKGLGLSGGPWYVIKKDIAANIVYVSCGYDVETQYGNEFS
                     MRNFHFITENPWQNQTEEANITFKIRHTPEFTKGKILHTNSGWHVISEKKIQGIAPGQ
                     FGVIYDKDSHICIGSGEIF"
     gene            11095..12321
                     /locus_tag="PLM04_05140"
     CDS             11095..12321
                     /locus_tag="PLM04_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HON02493.1"
                     /translation="MTYQTHTLPNGLRIIYLQKKSEVTFCGITINAGSRDENREEEGC
                     AHFAEHLLFKGTRKKNAREIINRMEDIGGELNAYTSKEETVVYAAAMKKYTRRMIELM
                     ADMTLHSTFPQKELEKEREVILDEIASYHDNPSELIFDDFEELIFKDHPLGHNILGNA
                     KLLKKMNSESVSNFVKRCYQPHQMIVFVVGNLNFEHFVKWCEEYFVSESAENINEKQK
                     RNPPEQYRAEKLKLKKKTHQIHTVIGNRCFDLYHPNRLPMYLLNNILGGPGMNSLLNL
                     SLREKHGLVYTAESNYQPFTDSGWWCVYYACDERNDEKCEQLVLEQLQKIVDRPLPES
                     KLRKYKQQLIGQMTVSRENTENFALSLGKSYLRFGKVEEMEEIKKQLENIQPKDLQYI
                     AQEIFNEEQLSVLKYY"
     gene            complement(12477..13460)
                     /locus_tag="PLM04_05145"
     CDS             complement(12477..13460)
                     /locus_tag="PLM04_05145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HON02494.1"
                     /translation="MTNYLFRTPVELAPSQQKITYKDKIMMLGSCFTENIGKQLHEAF
                     FQVDINPFGVLYNPLSISMSLERLIENKGFSRSDIFEYQSLWHSFFHSSSFSDVSPEK
                     TLSNIQARFDESCSFLKNTDFLLLTFGTAWVFEDRKTQTVVSNCHKLPSQHFNRRRLS
                     IEEIVEHYTLLIAQLQQLNPSLHLIFTVSPIRHWKDGAHENNLSKSILLLSIEELQKK
                     FKNVDYFPAYEIQMDELRDYRFYASDMLHPSETAINYIWEKFSDTYFSEKTKQIKQRL
                     EMLREKMNHRPLHPNSPEYVSFLQQIELEKNNILNEYPFLKDRIKDVNSRN"
     gene            13874..14440
                     /locus_tag="PLM04_05150"
     CDS             13874..14440
                     /locus_tag="PLM04_05150"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HON02495.1"
                     /translation="MPLDSVIPAFVREQSSDYRNNNLFMLNTLNLQNYFAFNNDHILE
                     GFSLWSDENIFSYGIKRYVFMGGAHGLSTQTFLNFDLKTGKTLTEKDLFIPDYKEKLT
                     TLIRERLIEENDEISEYRDLNQTDYWVDSIQPNGNFYISDEGINYLFNPYEIGPYYLG
                     ETEVRIPYARLKDILKNGNPLEYLYENR"
     gene            14452..14979
                     /locus_tag="PLM04_05155"
     CDS             14452..14979
                     /locus_tag="PLM04_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HON02496.1"
                     /translation="MNCFFNLAKSRRSIRKFLPQKVEPEKISMITKVALMAPASKRSN
                     PWEFVVVQQPEMLQKLSLCRPKNSQFLAGAPLAIVVLADTEKSNVWMVDAAIASIMMQ
                     LQAHDLGLGSCWIHVHDRQMDENTSSEDYIRGLLQIPTQYAILNILAVGYPNETKNPH
                     DEEKLAYEKVHFEKF"
     gene            14995..15318
                     /locus_tag="PLM04_05160"
     CDS             14995..15318
                     /locus_tag="PLM04_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02497.1"
                     /translation="MASKKKSSNLAWGITLLLFGLLFLLRQLQIVPPEIGVFVFDIKN
                     FPFIAGVIFLLFHPNISIGIVLLAVGVLFRLSEIIAWTKNFSEFILPALLIIAGSILI
                     FSKRK"
     gene            15404..16198
                     /locus_tag="PLM04_05165"
     CDS             15404..16198
                     /locus_tag="PLM04_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HON02498.1"
                     /translation="MKRVVIVGAGNVATHLGRALQNKNFVVSQIFSRTENSATQLASD
                     LGVPFTTDLQLLDREADIYFYCVKDAVLCDVINKIDIPDALHVHTAGSLPLSIFESHL
                     QYRRYGVFYPLQTFSKTRKVNFDEIPIFVEGNSLEAEEEIRQIAQTLSKRVFSSDSEQ
                     RKKIHLSAVFACNFVNHLFSIAEELVKTAGYSFDILLPLITETVNKLNELSPYDAQTG
                     PAVRNDQNIIRQHLLLLENKPEFSEIYSVLSESIYRMHKKDIQTRE"
     gene            16258..17244
                     /locus_tag="PLM04_05170"
     CDS             16258..17244
                     /locus_tag="PLM04_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HON02499.1"
                     /translation="MKQYLQLLRLPNLLFIAFVQFAMSEAVILPLLMTYGFDAGMSSS
                     KLLLLILSTILIAAGGYVLNDYFDVKIDTINRPDKMIVGKTIPRASAMRYYQILTALG
                     LLSGFILAFLVKSFALGFIFLVVPGMLWFYSATYKRQFLTGNIIVAFASALSVLLPAI
                     LELAVLESQYGKLIFETPIPKQIYAWVGGFAVFAFFCTLLREIIKDAEDIAGDREMEC
                     RTLPIVWGIKKTKIIVYCLILLIVAGLLAADFFLIDFSSSLTTRYLIFGLIIPFVCLG
                     YLIFKAENAHDFHQTSTLSKVIMLLGVSYSFVFYFLQAQQYHFPIFHIFMVQ"
     assembly_gap    17264..17363
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17487..18305
                     /gene="yaaA"
                     /locus_tag="PLM04_05175"
     CDS             17487..18305
                     /gene="yaaA"
                     /locus_tag="PLM04_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HON02500.1"
                     /translation="MAFSQKKKFTSKEFTAMLMLLSPAKIQNTKLPKNSYPATIPQFL
                     DDAEYLVEKVRELSLRELAQLLGVNSQLAELNRDRFYSWHRPFTVDNARQAVLVYNGE
                     VFRGLNAASMTENELTYLQQHLRILSGLYGVLRPLDLIQPYRLEISSRLPTFAGNDLY
                     DFWGKKITDAVSQAFQFSGQPEILLNLCSSEYIKAIDRDFFDGKIVDIEFLENDHGNF
                     RPIVVYLKKARGLMVRYVAQYRIENVEDLKGFDVEGYWYHPELSNDNKMVFVRG"
     gene            complement(18773..19831)
                     /locus_tag="PLM04_05180"
     CDS             complement(18773..19831)
                     /locus_tag="PLM04_05180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HON02501.1"
                     /translation="MSNLLIFGDNLKGLKYLLEEKELKGQIDLVYIDPPFATGGNFVI
                     SNGRATTISNSKNGDVAYSDNVKGSDFIEFLRQRLEILKDLLSERGSIYLHTDYKIGH
                     YVKVMMDEIFGVENFRNDITRIKCNPKNFSRIGYGNIKDMILFYTKTSNPIWNEPKEK
                     HKEVNIEKLYPKKDKNGRRYTTVPIHAPGETSSGDSSKPFKGLLPPPGRHWRTDIKTL
                     EKWDNEGLIEWSSTGNPRKIIYADESEGKRVQDIWEFKDPQYPDYPTEKNSDMLDLII
                     KTSSNENSIVLDCFCGSGTTLKSAHLNNRNWIGIDQSSLAIDAAKRKLGEISASLFVP
                     KPEYEFVDLMENLLAYNS"
     gene            complement(19836..20618)
                     /locus_tag="PLM04_05185"
     CDS             complement(19836..20618)
                     /locus_tag="PLM04_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HON02502.1"
                     /translation="MNKWINLSIEYANQKSYLDDLFQVYPTIPEGIREIDVQIWREVE
                     NAFIKRDNYRLIKELLKLDLFPIKDSYIAYLKRDSSAIDRNPRTINRICGRIYELGLN
                     KLYERCSEPKETNRQIGPMFKDWLRNKSLGILPVEIDEFMSGNNDAILDGGDKVLMDF
                     AKEKLGYYHDKGLDFVARFNGKYVIGEAKFLTDFGGHQNAQFNDAISTLEVKKVKAIK
                     VAILDGVLYIKGNNKMYKSITQKFNNYNIMSALVLREFLYQL"
BASE COUNT         6751 a         3597 c         3915 g         6430 t
ORIGIN      
        1 caaaccgctt tcctgttttg aaaaatgttg tctttatcgg acaggaaaaa cacaggggaa
       61 tgtatactac tgctgaaatt ctgcttcttg gaacttatca gaataatgaa gaatattttc
      121 aactgaaaaa aacgatcaat tgccatgatg tggtcaatat gcaatatact tccggaacta
      181 ccggtttccc aaaaggagta atgcttaccc atcacaacat aaccaacaac gggttttaca
      241 caggagaaca catgaaattt accaatgacg acaaactgtg tatctgtgtt cctcttttcc
      301 actgttttgg cattgtattg gcagtaatga attgtctgac acatggttgt acacaagtaa
      361 tggttgaaaa attcgatgca cttttaacgc tggcttctgt tcataaagaa cgttgcacgg
      421 cactttacgg agtccccact atgtttatcg ccgagctgaa tcatcccatg tttaatttgt
      481 ttgacctcac ctcattgcgt accggcatca tggcaggtgc actctgtccc atagaactga
      541 tgcgcaacgt aaatgaaaaa atgtatatga acatcaccag cgtttacgga ctcaccgaga
      601 catctcccgg tatgacgcaa acacgagttg atgattcgtt tgaagttcgc tgtaccaccg
      661 ttggaagaga atatgaattt gttgaagtgg cagttctcga tccaaaaaca ggcgctccct
      721 gccccgatgg tgtagaaggc gaaatgtgtt gcaagggcta caacgtaatg aaaggttact
      781 acaagaatcc ggaagccacc catcaggtaa ttgatgccaa tggatttttg cattcaggtg
      841 atttgggagt cagggacaag aacggaaatt atcgaattac gggacgaatt aaggatatta
      901 ttattcgagg tggcgaaaat atttctccca tcgaagttga agattttctt tatcaaatgc
      961 ccggaataaa ggacgttcag gtcgtggcca ttgcttcccc acgttacgga gaagatgttg
     1021 cagcttttat tatcaaaaag gatggatttg acattcggga agaagatgtc agagattttt
     1081 gcaaagataa aattgcccgt tacaaaattc cgcgttatgt attttttgtt gatgaatatc
     1141 cattgaccgg cagcggaaaa atacaaaaat tcaaactgcg cgaaatggct ttgcaaatct
     1201 gcaaggaaag aggaattgaa attgtttgaa tgtaacggtg aaaataaaaa tgtacctttg
     1261 taaggtttct tgatattttt tttcgcaatg aaaaaactat ggcgtattta cagatatttt
     1321 ttacatcgtt ttacagcaag aaacacgcat ggttttggcg tacattctcc attccttttc
     1381 cattttattt gttttgttgt tgaagaaaaa aatccctatt atattttttc ttccatcgaa
     1441 aatttgagag atcgcctcct caaagacaga acaaaaatac aaattgaaga tttcggtacg
     1501 ggtaaaagca ggacttcaac cattgcaacg gtagtcaaca aatcggtaaa atcaaaaaaa
     1561 tacggtcaac ttttttttcg gataacactc cacaccaaag cgcaatctgt tcttgaactg
     1621 ggaacttcac tcggtattac aacggcatat ctggctgcag cttcttctca gcttcattgc
     1681 ataagtctgg aagggtccca aacattggcc gccttggcaa aaaaaaattt ccaacaattg
     1741 ggactcaaga atatttcctt aatcgaaggg aatattgatg aaaacttgga gaaagttctt
     1801 gaaagtactc cgtcattcga cttaatattc attgatgcca atcatacgtc gcaagccgta
     1861 attgaatatt tcaacatgtg tcttgaaaaa attcacactc attccgtaat gctggttgac
     1921 gatatttatt ggtctgccga tatgctgaaa gcctggaaat acatcaaaca acatccaaaa
     1981 gtaacagcta ccatcgattt gtttgagaca ggaatcgtat ttttcgaccc caatctatac
     2041 aaaaaacact ataaagtgcg ttactaaatt gctattgcac aaaacatatt aatgtatttg
     2101 ttcaacaaaa aatcacgaaa aattgttatg tggtcagatt gctattgaaa aacaaatcaa
     2161 caaattaaaa aacaactgat tttcaaaaca atagaaaaat aaagaaacca aacagtatga
     2221 aactatatac aaaaacaggc gatgaaggtg aaacgggatt aattggagga acgagagttc
     2281 caaaaaatca cattcggctg gaagcttatg gtacggtaga tgagctaaat tcatttattg
     2341 gcttgttgac cacttttcag atagataatt cggatttact ttttctgcaa tccattcaaa
     2401 ataaactttt taccattggc tcttatttgg caaccgattt ttctaaattt tccaaaacta
     2461 aagtttctcc actttttgaa aaagacatca caagcataga aaaggaaata gaccgattga
     2521 cgtcggaagt tccggctttg aaaaattttg tcttgcccgg tggcagtcaa gcctcagcct
     2581 tgtgccatat ctgcagaacc attacacgtc gtttggaacg acgtttgtat gacttacatg
     2641 aacaaatacc tgtcgacaaa aatatactga tttatatcaa tcgcatgtcc gattatttct
     2701 ttgccttgtc gcgatatttg ttgtttaaaa acaatattca ggaaatatta tggcaaaaaa
     2761 atgaggaaaa cgattaattt gtaagttttt taattatttt tgcgcaaaat ttagaggaaa
     2821 aataaatatt caatcaaaaa aattgaacct aaatatttag ctatatgtat tggacgttag
     2881 aattagcttc aaaaatagaa gatgcaccct ggccggcaac taaggatgaa ttgatagatt
     2941 atgctatccg ctccggtgcg ccattggaag taatcgaaaa cttgcaggaa attgaagatg
     3001 aaggtgaaat ctatgaatcc atcgaggata tttggcccga ttatccgagt aaagaagact
     3061 ttttcttcaa tgaagaagaa tattaatctt tcaaaattct ttttattaca aaactttact
     3121 tatttttgtt gagctaaaag taaggattag gagtaatgga tacggtgaaa gtgcaatttt
     3181 tttgttaaat tgaagttatt tataaagttg acaagctttt ttgatcgaaa aaaaggttga
     3241 ataaaaactt aataataaga atcatctcat gaaaaaaata gtactcactg ttgttgtatt
     3301 tatggtttca tcagtgagta ttatttctca aactgcatca gagtggagcc agtacatgtt
     3361 taattcttct tccttaaatc cggcagcggt cggcgaaaaa ggaatgattg aagctgtttg
     3421 gcaacatcgt attcagtggg taggaatgcc caatgcgggt caatcggata ttattaacct
     3481 caacttgcct gttgattttt tgtccaccac tcatggtgtc ggtttaaaag tcatagatga
     3541 taaggtaggc ttattttcta acacttcagt atatctgcaa tatgcatata aactaaaaac
     3601 aacatcagga gtttggagtt ttggaacaga attgggtttg atcaatgcag ggtttgaggg
     3661 agacagcgtt cattcagtca acataggtga ttaccacgaa tcgaccgatc ctgtggtacc
     3721 atcaaccaat gttcaaggga tgggtttcga tattgggttg ggagcttggt actcttcgcc
     3781 aaaatttgag gcaggaattt cctacgcaca tctcaatcag cctgtcatta gttggagcga
     3841 ccaacttgat tatcaggaga aaggtgttct ttttatcaca ggtggttaca attggacttt
     3901 gattgatccg aaaatggtta taaaaccgac aacgctcttt aaaactgatt ttgtttctta
     3961 tcagtgggat gcttctgcac gtatggaata tgacaataaa tactggggag gactttccta
     4021 ccgatggggc gatgcggtgg tattttttgc tggaatcaat gccattgctg gtttaaatat
     4081 tggttatgct tatgatttac cggtttcgca aattataaag gcaagttccg gatctcatga
     4141 atttttgata agttatagtt ttgaatataa attaaataaa gcaaaacaca agtacaaaag
     4201 tattcgtatt ttgtgacaaa agtatttttt cagaaaaaag attaaaaaac aatcacttta
     4261 ttgttataca aggatgaaaa gaaatgtgct aattagtatc cttttcggat cattgcttct
     4321 ggccggatgc aacaagcctg ccggagaatt gattggagtt gggaacagag gaaattttcg
     4381 cgaagccaat ccctatggta tggttttcat aaaaggaggt tcgtttttga tgggagccaa
     4441 tgaccaatca gcaattggag ctatcaatga caagtcgata cacgtaacgg tcgacgcctt
     4501 ttggatggat cagaccgaaa ttaccaatga cgagtacaaa caatttgtga attgggtgcg
     4561 tgattccatc attttacggt ctcttgtaat ggctggccga gacgaatttc gcctgaagtt
     4621 gaggaatcag gatgagcttc cacctgaaac ggcaccttta aattggaaag cgaaaattcc
     4681 gtggaaatcc caagatgaag aaattagaga ggtgctcaat acattttatt atgaggacgt
     4741 aaactcactt tcaggcagac agttgaatcc aaccaaattc cgttatcgtt atgaatggat
     4801 taattatgat caagctgctt tggccagcaa taaatatgat gtaaacacag gagcctatcc
     4861 aaaaaatgca aaagttagag ttgacacctc ttatgttgac gaaaacggag tgattcatca
     4921 tcaaactatc gaacgtccct tgagaactcg taaagatttg atttccacga aaatagtaaa
     4981 tatatatccc gatactatca tgtggattag agattttcag tatgcctaca acgagccaaa
     5041 aatgaaaatg tacttttccc atcccggctt tgccaattat cctgtggtag gggtaacatg
     5101 ggaacaggca caggcatttt gtcaatggag aacgcatatt tacaacggta caaacaaaat
     5161 cagtgctcag gactatcgtt tgcctaccga agccgaatgg gaatatgcag ctcggggtgg
     5221 tcgtcaaatg gctttgtatc cttggggtgg caattatgta agagacaaaa gaggatgttt
     5281 tatggctaat tttaaaccca tgcgtggaag ttataacgat gataccggaa caacaaccat
     5341 gaaagttggt tcatttcttc cgaacgattt tggactgtac gatatggcag gaaatgtagc
     5401 agaatggact tcttcagctt tcaatgtctc cagcagcaac attgtttacg atattaaccc
     5461 gaattttcag ttcaatgcaa aaaacagcga tcctcaagta ctgaaacgca aagtggtaaa
     5521 aggcggatca tggaaagaca ttgcctacta cctgcaatgc ggtgcacgta cctatgaata
     5581 tcagaacgaa agcagacctt atattggatt tagatgtgta cgatcatata atggcgaata
     5641 aaagtgtaaa ctttaataat tacaaacatg tcaaataatg ctataaattc aaaaaaacaa
     5701 tcaggatttt ccgaatggtg gaattctccc aaagggagaa aaattgttaa tgccatatac
     5761 agcgttggag ccggagtagt tatcatcggt gcaatgttta aaatactgca cttgccgggt
     5821 gcaaacgtgg tgctaggtac cggcatgatt tgtgaggcaa ttattttttc aatgggagca
     5881 ttagataaac cttttaaaga ataccattgg gataagattt tcgattttga aaatggaaca
     5941 gcccgatttc aaactgaact aataggaagt gcgacaatat ccgcttctcc ggctcatcca
     6001 tctataaatt attctgaaac gctttctgaa gaagatgtag aaaaattatc ggaaggaata
     6061 aaaaatcttt ctgccacagc acgacaacta gcatctgttt ctgatttaac tgccccaact
     6121 tcggagttta tccgcaacat aacttctgct tctgaatctg taagcaattt ttcgaaagac
     6181 caaaataacc tgaactcttc tcttcgtaat ttatcaggga tttatgtgga aattggtact
     6241 gaaatgaaaa aagcagaaga gaacacaaaa gattacactg gggaaattga gaaaatcaac
     6301 aagaatttaa gttcattgaa tgccatttac gaaattcatt tgaaaaacgt ggaagaacaa
     6361 tcaactaaaa taggaaattc caacaaggaa ttggaaaata ttctatcgaa cttgaaaaaa
     6421 attaattcat tttcctctgc aagtgctgaa gaatcagaaa agtataaaaa cgaaactgtt
     6481 aaactggcac aacaaatttc tgatctcaac aaagtttatg gaaatatgtt gaatgcatta
     6541 agctgataac aaagagattt ttatttttta aattttctta aaataagaca ttagaaataa
     6601 aataaaagat gagtggagca aaaaattgtc ccgagaccac ccgacaaaaa atgatcggga
     6661 tgatgtattt ggtgctgaca gccatgctgg cattgaatgt atcaagtgaa gtgctggaag
     6721 ggtttactat ggttgacaac agcttgcata acactatcat ttcagcttcg gtaaggaatg
     6781 aagatttata caaacaattc gaatatttat acaatcagaa tcccacaaaa gtaaaagaat
     6841 ggttagataa agctttggat gttaaaaaac aatcggattc tctttacaac tatattcaaa
     6901 atttcaaata tgaaatattg aaaatggttg ataataaaaa tgctgatcct gaagcgcgta
     6961 gggtgattaa taaagaaaat tacgatgcac ccggtgaata tgcattggta aaaggtaatg
     7021 ggaaaattct taaggaaaaa attgataatt atagaaattt tttgatctca ttatctggca
     7081 atagtccggc aaaacagcaa atgtatgcta ctatattttc aaccgctcca tcagaaaacg
     7141 gagtaccttg ggaatatgct ttgtttgaaa tgatgcccgt ttcggcggtt ataaccgtac
     7201 taacaaaata tcaaagcgat attcgaatgg cagaagccga attaatccag tacctcaaag
     7261 cacaaactga cgtttccgat ttcagggtta ataaaattga agccttagtt gttccaaatt
     7321 ctcgttacgt atttagagga agcaaatatt cggctcaaat tgttctttca gccgttgatt
     7381 ccactaaaac gcccgagttc tacgtaggta atacccctgt aaaaaatgga atttatgaag
     7441 ttaattgcaa tcagacaggt agctttactt atactggttt cgttcagtta atagggaacg
     7501 atggtgtgat aagaaagttt ccctttacaa gtgattatat tgtcggtgag ccttcggcaa
     7561 ccatttccaa tgaagatctg aatgttgttt atcgcggcat cgaccataaa ttcagtatct
     7621 ctgttccggg agtagcctct gaaaatattt cagtaagggt ggcgggagca accgttcaaa
     7681 aaactgctgg aggtaaattc attattcgtc ccacacagga tggggaaatc aatgttgctg
     7741 tgtatgccaa agttgaagga aaagaaattt taatgggtaa cagtatttat agagtgagat
     7801 atttgcctga tccaaaagct tatttacaat attcggatgc cggtggaatt acaagattaa
     7861 tacaggatgg aaatcttaat aagcgtttat tgagaggcga caatgtagcc attgtggcaa
     7921 gttatggtga agacgaactc attcaggcaa aattcaatat tatttctttt acaatgctca
     7981 ctatatatgg ttcagtagaa accaccggat caaggttaag taatagacaa ttggctgata
     8041 ttgatcgttt ggagggtgga gattatatta catttaaaaa tattaaagcc gtcggtcctg
     8101 atggcaaagt aagaaatctt ggattagttc aagtacaaat ttagtttttt aaaatttttt
     8161 gcaaaaataa gcaaaccaaa tagaatgttc agtatgaaaa aaattggtgt tctaacttta
     8221 ttcttggctt ttattctact accgggttcc ataaaagccc aaattaatga aattcctttt
     8281 tttgatagca aaggaaatgt taacattgaa actaccgagc tcgatacatt ggcggacacc
     8341 attgcagttg ttcatcatcg tagagacgat attgtttggt cgagagtggt atatagggtt
     8401 atagatatgc gagaaaaaca aaactatcaa ctatattttc caactcgacc caataaggaa
     8461 tatcgcaatc tttttacggt aatgatcgac gcaatttgtg atggagttaa cgtgtataaa
     8521 aaaaatccgc gcgatatcaa acctgaattc aacgaattgt tggaagggga agaattatcc
     8581 agggcttttg cttttgaaga agacaatacc agcaatctta ttcaaataga tcccataacc
     8641 aatcagcgaa ccttcaacaa ttaccgttat attcagtacg tgcgcaatca gttgaaatat
     8701 ctgattcagg aaattatttt cttcgataag catacctcca gaatgtattc caaaatcatt
     8761 ggtattgccc ctctttatgc tttacatccc gataatgtcg aatccaatga gacaatggga
     8821 tatttcagag gttccgttct ctgttggttt gcatttgacg aactgcgtcc ctatctggcc
     8881 aaacaatatg ttatcccaaa tgggaacgac acccaacgca tgacttttga cgactttttc
     8941 tcgcaaaaac tctatgcaag ttacttgctg ggcgacagca acatgtttaa cagaatgctg
     9001 ctcgattatg ttattgaccc tgccaaagtt agaaaagaac aaaacaggat agaaaacgaa
     9061 ttgctgaact ttgaacagga tttgtgggaa tattgaaaaa agctttcaaa tattattcaa
     9121 atattcaaaa agacggctgt tgagtcgtct ttttttgctt aacgtatgaa actacattgc
     9181 aaaaaatttt tttttaaagt aaattttatc gaacaagtga acaaaattat tcttaaacga
     9241 ttataatcaa aaaaacacaa acgtcgtaaa gattttttaa gaaaaaagtt attcataaaa
     9301 aaatgaagaa tatcatactt tccgataatc aatatatttc taaagccggg tggctttatc
     9361 tgatctcaac ctactctctg gaggcaacca ttagggaagc agccacaaaa aaagaactgg
     9421 aaaaactcct tgttgaagag caggaatcat tggtaatatt ggattataca ctttttgatt
     9481 ttgaaaacgt caatgaattg ataatatgga gcatgcgttt tgaaaaaacc gagtggttat
     9541 tattttccga agaattaagc gaagaatttc ttcaacaaat tatttacaac acccactcct
     9601 tcagtgtact cacaaaaaac agcaataaag aagaaataaa tctggcatta aaagaaacat
     9661 tgtctggtaa caggtttatt tgtaactttg tatccaattt gctgattgac tgccaaaaac
     9721 atctgaaaaa tgatggcaaa ccggttttaa cacctactga gcgtgaaatt ctgaaagaaa
     9781 ttgcactggg aaaaaccacg aaggaaatag catccgagcg ttttgtgagt gtacacacca
     9841 ttatgacaca cagaaaaaat attttccgca aaataaaagt caataacgta cacgaagcca
     9901 caaaatatgc aatgcgggct ggaattgtca atctggctga atattacatc taaaatcagg
     9961 ctaaaaaatg aaaatagcgg ctttactttc cggaggggtt gacagttcgg tagtagttca
    10021 tttgctgaag gagcagggtt acactccttc gctgttttat attaaaatcg gaagagacga
    10081 ggacgaaatg ctgaattgct cttcggaaga ggatatagaa atggcttcac tcgtagcaca
    10141 gaaatatgat tgcaagctcg aaattatcga cctgcacaag gattattggg aaactgttgt
    10201 tgcctacacc ataagcaagg tgaaggaagg attgacccca aatcctgacg tgatgtgcaa
    10261 caaactgata aaatttggca tttttgaaca aaaagttggg aaagaattcg acaaaacagc
    10321 caccggacat tatgccacca tcaccgaaaa agatggaaaa atttttcttt ccactgcaaa
    10381 agatccgata aaagaccaaa cggattttct tgcacaaatc gattatctgc aggtttcaaa
    10441 actgatgttc ccgattggac acttcatgaa aagtgaagtt agaagcattg cacaaaaggc
    10501 cggtttgcca agtgccaaaa gacaggacag ccagggaatc tgttttctgg gaaaaatcaa
    10561 ttacaacgat tttattcgtc gctacttggg agaaaaacca ggtcctattg tggaatggga
    10621 aacgggaaaa attcttggga agcacaaagg atattggttt cataccattg gacaacgcaa
    10681 aggtcttgga ctatcgggag ggccgtggta tgtgataaaa aaagatattg ctgccaatat
    10741 cgtttacgtt tcctgcggat atgatgtaga aacacaatat ggaaatgaat tttcgatgcg
    10801 caattttcat ttcattaccg aaaatccgtg gcaaaaccaa acggaagaag caaatataac
    10861 tttcaaaatc agacatacac ccgaatttac gaaaggaaaa attctgcata ccaacagcgg
    10921 ctggcatgtg atttccgaaa aaaaaattca gggtattgct ccgggacaat tcggcgtgat
    10981 ttacgacaag gattcccata tttgcatcgg cagtggtgaa atattctgaa aaaacatccc
    11041 gtcttttttc acacaaatgt aaccatcaat tatttcaata aacaaattct gcagatgact
    11101 taccagaccc atacgcttcc taatggttta cgaatcatat atctccaaaa gaaaagtgaa
    11161 gttacctttt gcggcattac gataaatgcc ggttcacgcg acgaaaatcg ggaagaagaa
    11221 ggatgcgcgc attttgccga acatttgttg tttaaaggta cacgaaaaaa aaatgcacga
    11281 gaaatcatca accgaatgga agacatcgga ggagaactga atgcctatac atccaaagaa
    11341 gaaacagtgg tgtatgcagc ggccatgaaa aaatacaccc gtcgcatgat tgaattgatg
    11401 gccgacatga ccctacattc cacctttcct caaaaagaac tcgaaaaaga gcgggaagta
    11461 atcttggacg aaatagcgtc ctatcatgat aatccgtcgg aacttatttt tgacgatttc
    11521 gaagaattga tttttaaaga tcatcctctg gggcacaata tattgggaaa cgcaaaatta
    11581 ttgaaaaaga tgaattcgga atcagtcagt aattttgtaa aaagatgcta tcaaccccat
    11641 caaatgattg tttttgtggt tggaaacctg aattttgaac actttgtaaa atggtgtgaa
    11701 gaatattttg tatcggaaag tgcagaaaat attaacgaaa aacaaaaacg caatccccct
    11761 gaacaatatc gggcagaaaa attgaagctg aagaaaaaga cccatcaaat ccataccgta
    11821 attggaaatc gctgtttcga tttgtaccat cccaacaggt tgcccatgta tttactcaac
    11881 aatatacttg gggggccggg aatgaatagt ttgctcaatt tatcgctgcg cgaaaaacac
    11941 ggacttgtct ataccgcaga atcaaattat caacctttta ccgacagtgg atggtggtgt
    12001 gtttactatg catgcgacga aagaaacgac gaaaaatgtg aacagctggt tttggaacaa
    12061 cttcaaaaaa tcgttgaccg acccttgccg gaaagtaaac ttagaaaata caaacagcaa
    12121 ctgatcggac aaatgactgt ttcaagagaa aacactgaaa attttgcact tagtttggga
    12181 aaaagttatt tgcgcttcgg gaaagtggaa gaaatggaag aaataaaaaa acagttggag
    12241 aatattcaac ctaaagattt acaatacatt gctcaagaaa tttttaatga agagcaacta
    12301 tccgttttga aatattattg aaattatctt ctacaaaaaa aaatggatcg aaaatccgaa
    12361 aaggaaaaac cgagtttgct tttttgcctg attatggatg tattgggttg cattactaca
    12421 ttatgccttg aaaaataatt tccatttacc ttttatttcc tctgaaaaaa gttgcattaa
    12481 tttcttgaat ttacgtcttt tattctgtct ttgagaaacg gatattcgtt caggatattg
    12541 tttttttcca attcgatttg ttgtagaaaa gaaacatact ccggtgaatt gggatgcagt
    12601 gggcgatgat tcattttttc cctcaacatt tccaatcgtt gttttatttg ttttgttttc
    12661 tccgaaaaat aagtatccga aaatttttcc caaatgtaat tgatggcggt ttccgaagga
    12721 tgtaacatgt ccgaagcata gaaccgataa tcacgcaatt catccatctg aatttcatag
    12781 gcagggaaat aatcaacatt tttgaatttt ttctgcaatt cctctatcga taacagcaaa
    12841 atgcttttac ttaagttgtt ttcgtgagcg ccatctttcc aatggcgtat agggctgact
    12901 gtaaaaatga gatgaagcga tggattcaat tgttgaagtt gggcgatgag caaagtgtaa
    12961 tgttccacta tttcttcaat ggatagcctt cggcggttga aatgttgtga aggtagctta
    13021 tggcagttgg aaactacagt ctgtgttttc cggtcttcaa aaacccacgc tgtgccaaag
    13081 gtcagcaaaa gaaaatctgt atttttcaga aatgaacaag attcatcaaa ccgggcttga
    13141 atattagaga gtgttttttc gggtgaaaca tcagaaaatg aactactgtg aaaaaaacta
    13201 tgccaaagag attggtattc aaaaatatct gaacgtgaaa aacctttgtt ttcaattaat
    13261 ctttcaagac tcatagagat ggaaagcgga ttgtaaagca ctccgaaagg attgatgtca
    13321 acctgaaaga aagcttcatg aagttgttta cctatatttt cagtaaagca agagcctaac
    13381 atcatgattt tatccttata agtaattttt tgttgggatg gagcaagttc aacaggggtt
    13441 ctaaaaagat agttagtcat aaaatttact ttatttttaa aaatttgtca gatttcatat
    13501 taactttaac agattataaa cgtattttcc gtaaaaaatc atttatttat tttacaaaag
    13561 taataaatgt tcatagttat tgttgtaaat ttgtagctct aaaaatgata tacaaaatat
    13621 gaaaaagaac aaaaacatct tattgtttat ctgtttttta tcgatttctt ttttttgggg
    13681 gtgtgtgtca aaaacagtta aaacaaaaaa tttcgagtat gcgaataaat tttacctcac
    13741 cgatgattca acaaaaggag ctttaaattt gattattaat gttgagatac cagtaaattt
    13801 ttctaataaa accgttttgg attcagttcg caataaaata ttgttgtttc tatttggaga
    13861 aaattatgtg aacatgcctt tggattcggt tatcccagcc tttgtccgag aacaaagttc
    13921 cgattatcga aataacaatc tttttatgtt gaatacactc aatttgcaga attattttgc
    13981 tttcaacaat gaccatattt tggaaggatt ttcgttgtgg agtgacgaaa atattttttc
    14041 atacggaata aaacgttacg tattcatggg tggagctcac ggattatcaa cacagacttt
    14101 cctgaatttt gatttgaaaa cgggaaaaac tcttacagaa aaagacttgt ttattcctga
    14161 ttataaagaa aaactgacta ctttaattcg tgaaagactg attgaggaaa atgacgaaat
    14221 ttcggaatat agagatttga atcaaaccga ttattgggtg gactccatac agcctaacgg
    14281 gaatttttac atttcggatg aaggaatcaa ctatttgttt aatccttatg aaattgggcc
    14341 ttattatctg ggcgaaacag aagttcggat tccttatgct cgattaaaag atattttaaa
    14401 aaatggtaat ccgttggagt atctatatga aaatcggtaa gttaaatgaa tatgaattgt
    14461 tttttcaatt tggccaaatc gcggcggagc attcgcaaat ttcttcctca aaaagttgaa
    14521 cccgaaaaaa tttcgatgat aacaaaagtg gctttgatgg ctcctgcatc gaaaagaagc
    14581 aatccctggg agtttgtggt ggttcaacaa cctgaaatgt tacaaaaatt atcgctgtgt
    14641 cgtcccaaaa attcgcaatt tctggcgggg gctccgttgg caattgttgt gctcgccgat
    14701 acggaaaaaa gtaatgtttg gatggttgat gctgctatcg cttccattat gatgcagttg
    14761 caggcacacg atttgggatt gggcagttgc tggattcatg ttcatgaccg gcaaatggat
    14821 gaaaatacaa gctcggaaga ttatattcgc ggtttgctgc aaattcctac tcagtatgca
    14881 attctgaata ttctggctgt gggttatccc aacgaaacaa aaaatccgca cgacgaggaa
    14941 aaactggctt acgaaaaagt tcatttcgaa aaattctaac tttattttta aaatatggct
    15001 tccaaaaaga aaagcagtaa tctggcctgg ggaataacat tactgttatt tggcttgttg
    15061 tttcttttac gtcaattgca gattgttccg cccgaaatcg gcgtttttgt ttttgatatt
    15121 aaaaattttc cgtttattgc cggagttatt tttcttcttt ttcatcccaa tatcagtatc
    15181 gggatagttt tactcgcagt aggtgttctg tttcggcttt cagaaattat tgcatggaca
    15241 aaaaattttt cggaatttat tcttcccgct ttgctgataa ttgccggtag catattgatt
    15301 tttagtaaaa gaaaataatc aattgccaca ggtgagtcaa attttttgta agtatctatt
    15361 ccttaatgct tttaaattct gatacagtta tagttttttc atcatgaaac gggttgttat
    15421 tgtcggagcg ggaaatgtag ctactcattt ggggcgagcg ttacaaaata agaattttgt
    15481 tgtgagtcag atttttagtc gtactgaaaa ttctgcaaca caacttgctt ctgacttggg
    15541 agttcctttc acaacagact tgcaattact cgacagagaa gccgacattt atttttattg
    15601 tgtaaaagat gccgtattgt gcgacgtcat caataaaatc gatattcccg acgctttgca
    15661 tgttcatacg gccgggagct tgcctttaag cattttcgaa agccatctgc aataccgtcg
    15721 atatggagtt ttttatccac ttcaaacttt ttcaaaaaca agaaaagtta attttgatga
    15781 aattcctatt tttgtggaag ggaattcatt ggaagcagag gaagaaatca ggcaaattgc
    15841 gcaaacatta tccaaaagag ttttttcttc cgattcggaa caacgaaaaa aaatccatct
    15901 ttctgccgta tttgcctgca attttgtcaa tcatcttttt tccattgcag aagaattggt
    15961 gaaaactgcc ggatattcct ttgatatttt gttgccgttg attactgaaa cagtaaataa
    16021 actgaacgag ctgtctcctt acgatgcgca aaccgggccg gccgtgcgaa atgatcaaaa
    16081 cattattcgt cagcatttgt tgttgcttga aaataaaccc gaattttcgg aaatttacag
    16141 cgtgttgtcc gaaagcattt atcggatgca caaaaaagat attcaaacaa gagaatagtc
    16201 aattttattg taaatcagaa aattgccttt tctttttatc agctaaattc aatatacatg
    16261 aagcaatatt tgcaattact tcggttacca aatttacttt ttatcgcttt tgttcagttt
    16321 gccatgtccg aagcggttat tttgccgctt ttgatgacct atggttttga tgccggcatg
    16381 agttcatcca aacttttgtt gttgatattg agtacgatat taattgctgc cggaggatat
    16441 gtattgaacg attattttga cgtaaaaatc gataccatca atcgtcccga taaaatgatt
    16501 gtgggaaaaa ccattccccg tgcttctgcc atgcggtatt atcaaatttt gacggctctg
    16561 ggattgttgt cagggtttat tcttgccttt cttgtaaaaa gttttgctct tggctttatt
    16621 tttctggtgg ttccgggtat gttgtggttt tattcggcca cctacaaaag gcagtttctg
    16681 acgggaaaca tcattgtagc ttttgccagt gctctttccg tacttttgcc tgccattctc
    16741 gaactggctg tattggaaag tcaatacgga aaattaattt ttgaaacgcc tattccgaaa
    16801 cagatttatg cttgggtagg aggatttgcc gtttttgctt ttttttgtac tttattgagg
    16861 gagattataa aggatgcgga agatattgca ggcgaccggg aaatggaatg tcgtactttg
    16921 ccaattgttt ggggaataaa aaagaccaaa attattgttt attgccttat tttgctcatt
    16981 gttgccggct tgctcgcagc cgactttttc ctcattgatt ttagtagtag ccttactacc
    17041 agatacctga tttttggttt gataattcct tttgtttgtt tggggtatct gatttttaaa
    17101 gctgagaatg cccacgactt ccatcaaact tctactcttt caaaagttat catgttgttg
    17161 ggtgtttcct atagctttgt gttttatttc ttgcaagccc agcagtatca tttccctatt
    17221 tttcatattt ttatggttca ataaatcggg aaaaatcatc catnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnncatttcc ctatttttca tatttttatg gttcaataaa
    17401 tcgggaaaaa tcatccatca ctgtcattca ttgacaattg gtaatcgggc tttatcgttt
    17461 tatgataaaa ttgaactaac acgtttttgg ctttttctca aaaaaaaaaa ttcacctcaa
    17521 aagaatttac agctatgtta atgttgcttt ctcccgcaaa gattcaaaac accaaactcc
    17581 caaaaaattc atatcctgcc accataccgc aatttttgga tgacgccgaa tatctggttg
    17641 aaaaagttcg cgaactttca ctgcgggaac tggctcaatt gcttggggtc aactcacaat
    17701 tggccgaact caatcgcgat cgtttttaca gttggcatcg cccttttact gtcgacaatg
    17761 caaggcaggc tgtgttggtt tacaacgggg aagtttttag aggattgaat gctgcttcca
    17821 tgacagaaaa tgagttaacc tatttgcagc agcatttaag aattttatcc ggtttgtacg
    17881 gcgttttgcg tcctctcgat cttattcagc cttatcgctt ggaaattagc agccgcttgc
    17941 caacttttgc aggcaatgat ttatacgatt tttggggaaa gaaaatcaca gatgccgttt
    18001 cccaagcttt tcaattttcc ggtcaacctg agattttact taatctctgt tccagtgagt
    18061 atattaaagc catagacaga gattttttcg atggtaaaat tgttgatatc gaatttttgg
    18121 aaaatgatca tgggaatttt cgtccaattg tcgtctattt gaagaaagca cgcggcttga
    18181 tggtgcgcta tgttgcccaa tatcggatag aaaatgtgga ggatttgaaa ggttttgatg
    18241 ttgaagggta ttggtatcat ccggaattgt ctaacgacaa caaaatggtt tttgtcagag
    18301 gataaggcaa attctctcgt tgtttttcca caaatctatg ccgattaaat ctcatttaaa
    18361 gcaaaatcaa acaatttaga aataaattaa aaagacattt agatacattt ttgcttcaaa
    18421 agtggaagca ttttttggct ttatcaaaca taaatactac tgtccgataa aaaaatagaa
    18481 aaggaaagaa taaaaaaggg gaagttactc aaattagata acttccccga catatagtat
    18541 ttttgttttt gcacaaacat aaaaactata tgggcaaaaa tccagaaaga aatttggtct
    18601 ttcagccgga ctttcggatt gacatttaat gaagttcttt tgattgatct tacgacgata
    18661 aggctattta gggacattct caaggaagtt gggcgaaatg cccaaaaagc ccgacacaaa
    18721 aaaaatggtt ttttccattg tggcaacatt agtacggata gatttaatca atttatgaat
    18781 tgtacgccaa caggttttcc atcaaatcga caaattcata ttcaggcttg ggcacaaaca
    18841 aactggcaga aatttcaccc aattttcttt ttgctgcatc tattgccaga gatgactggt
    18901 caatgcctat ccaatttctg ttgtttaagt gagctgattt taaagttgtt cctgacccac
    18961 aaaaacaatc gagtacaata ctgttttcgt tcgaggaagt tttaataatt aaatccagca
    19021 tatctgaatt tttttctgta ggataatcgg gatactgagg atccttaaat tcccaaatat
    19081 cctgcaccct tttcccttca ctttcatctg cataaataat ttttctcgga ttacctgtcg
    19141 aagaccattc gattaagcct tcattatccc atttctcaag cgttttaatg tctgtacgcc
    19201 aatgccttcc gggcggaggc aataaacctt taaaaggttt actcgaatct ccacttgatg
    19261 tctcaccagg agcatgaatc ggaacggttg tgtatcgcct tccgtttttg tcttttttcg
    19321 gataaagttt ttcgatatta acttctttat gtttttcttt tggctcattc catataggat
    19381 ttgatgtttt ggtatagaat aaaatcatat ccttaatatt cccataacct atgcgagaaa
    19441 aatttttcgg attacattta attcttgtaa tatcgtttct gaagttttct acaccaaaaa
    19501 tttcgtccat cattacttta acgtaatgtc ctattttata atcagtatgt aaataaattg
    19561 agcctcgttc tgaaagcaaa tcttttaaaa tttcaagcct ttgccttaaa aactcaataa
    19621 aatcactacc tttcacgtta tcggaatatg caacatctcc attcttagaa ttgctgatag
    19681 ttgttgccct cccgttagaa ataacaaaat tgcctcctgt agcaaatggg ggatcaatat
    19741 acaccaaatc tatttggccc tttaattcct tttcttccaa taaatactta agtcccttaa
    19801 gattgtctcc gaaaataagt aggttgctca tagatttata attggtaaag aaattctctt
    19861 aatactaaag cgctcataat gttgtaatta ttaaactttt gagtgataga tttatacatt
    19921 ttgttgtttc cctttatata taaaacacca tcaagaatag ccaccttgat cgctttaact
    19981 tttttaactt caagagtaga aatggcatcg ttaaattgcg cattttgatg tcctccgaaa
    20041 tcagttaaaa acttcgcttc acctattaca tacttcccat tgaatcgggc aacaaaatca
    20101 agtcctttat catgataata acccagcttt tctttagcaa aatccattaa aactttatcg
    20161 cccccatcca aaatcgcatc attattaccg gacataaatt catctatttc aacaggcaaa
    20221 attcctaaag atttgtttcg caaccaatct ttgaacattg gcccaatttg tcgattcgtt
    20281 tctttgggct cgctacatct ttcataaagt ttattgagcc ctaattcata aattcttcca
    20341 caaattctgt tgatggttct tgggttccta tcaatagctg aactatctct tttcaaatac
    20401 gcaatataac tatcttttat aggaaaaaga tccaatttta aaagttcttt tatcagtcga
    20461 taattgtccc tctttataaa ggcattttca acctctctcc atatctgcac atctatttct
    20521 cgtattcctt ccggaattgt tggatatact tgaaataaat catcgagata tgatttttga
    20581 ttggcatatt caatactaag attaatccat ttattcattt ataaataatt tttaaatatc
    20641 aatttgaaaa acttgctcat atattttccc ccttccgaat accttacaaa tttaagcgaa
    20701 aattctcaat tttcataccg tcaaaagctc gttgattttt taagcatatt cattcaggca
    20761 attagtatta ttcgttagat tacaaaattt tat
//
LOCUS       DAOTAX010000035        20721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2109_length_20721_cov_31.115697, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000035 DAOTAX010000000
VERSION     DAOTAX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20721
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_20721_cov_31.115697"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..749)
                     /locus_tag="PLM04_05190"
     CDS             complement(111..749)
                     /locus_tag="PLM04_05190"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HON02503.1"
                     /translation="MTVIHFFVLKYSTVEFIGLFISLFGLFIRAYAVGHTPANTSGRN
                     TKEQLADELNTTGIYSMVRHPLYLGNFFMWLGAAVLTKNGWFIVAFILLYWIYYERIM
                     FAEEQFLRRKFGSVYTDWANKTPAFIPSFKKYIPSKYPFSWKKVLKKEKNGLLAVFVL
                     LFVFHNINYSFLTNHIRIDLNWIAWGTFAAGILYLVLKFLKKKTSILNETGR"
     gene            complement(947..1609)
                     /gene="ung"
                     /locus_tag="PLM04_05195"
     CDS             complement(947..1609)
                     /gene="ung"
                     /locus_tag="PLM04_05195"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HON02504.1"
                     /translation="MDVKIESSWKEVLQSEFEKEYFKQLTDFVRKEYKTKKIFPPGSL
                     IFNAFNLCPYDKVKVVIIGQDPYHGPGQANGLCFSVNEGVDFPPSLVNIYKEIENEFA
                     KPMPKNGDLSSWAKQGVLLLNAILTVQAHLAGSHQGKGWETFTDAVIRILAEKKEHLV
                     FLLWGSYAQQKGLYIDTNKHLVLKSPHPSPLSAYRGFIGNRHFQLANQYLKEHGMSEI
                     EW"
     gene            2048..2320
                     /locus_tag="PLM04_05200"
     CDS             2048..2320
                     /locus_tag="PLM04_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HON02505.1"
                     /translation="MTKAEIVNEIAKKTGIDKATVLASVEAFMDAIKDSLSKNENVYL
                     RGFGSFVVKTRAQKTARNISKNTTIIIPPHNIPSFKPAKKFMSVVK"
     gene            2555..4129
                     /locus_tag="PLM04_05205"
     CDS             2555..4129
                     /locus_tag="PLM04_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HON02506.1"
                     /translation="MNSELVVDVKPSEISIALLEDKRLVELAQETREEQFAVGNIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFIHYLDLGPNFNTLSQFTLQALSDPKKAPSMQKVKM
                     LPEIEKSGFINDLLKPGQQILVQVAKEPISTKGPRLTAEISLAGRFLVLIPFNDKVLI
                     SQKIKSENERVRLKQLIQSIKPKGFGVIVRTVAEDKKVAELINELNVLVKRWDEAVAK
                     LQQAKGVTLISEELGRTVGIIRDLFNPSFKNIYVNDPNVFAEVQDYIEWIAPEQKNIV
                     KLYKDETPIFDHFGITKQIKSSFGKTVSFKNGAYLIIEHTEALHVIDVNSGNRAKTGN
                     GQEANALEVNLAAADEIARQLRLRDMGGIIVVDFIDLHESDNRQKVYDRMKEMMANDR
                     AKHNILPLSKFGLMQITRQRVRPDTHVDIEEACPTCFGTGKIKPSILFTDLLESKIDY
                     LVNTLKVKEFTLYVHPYVEAYISKGMIPLKNKWKIKFGRGCKVRASQELGFLQYKFID
                     NKGMELNLQEEIEKIS"
     gene            complement(4210..5031)
                     /locus_tag="PLM04_05210"
     CDS             complement(4210..5031)
                     /locus_tag="PLM04_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HON02507.1"
                     /translation="MNDDNITNHEQNINKTGICLSGGGALGFAHIGVLQALEENGIFP
                     VQIAGSSMGAIVGTLYAAGFTPAQMLQMIREDKLYKITKLMSIHPMSLKSGLSTHKML
                     RDLIKELIPHNSFELLKKKMSVCVSNLNKAEWEIIESGKELDKWVAASASIPGVFEAI
                     QVKKEYYVDGGLLNNMPAQALKENCSTIIGVDVIPHLNEVQLKKPIDTLTYSIRIIQH
                     KNAQKGRELCQYLIEPKAIRKFHEFSFDAYQQIYEEGYQCTHTFIENNPAINNGI"
     gene            5197..7365
                     /locus_tag="PLM04_05215"
     CDS             5197..7365
                     /locus_tag="PLM04_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HON02508.1"
                     /translation="MKRIFLAFVLAFAVQFSFADLLDDIVDGKFNVNNIMVPRSMKDG
                     ENYTLMKDNAVLKYNYKTGQLVDTIFSIHRLKDAPFKEINGYEFSPNEAKLLVYNNVK
                     KRYRRTFTADYYVYDIKRNEIQPLSDNGSQEVPLFSPDSRYIAFGRNNNLYLKKLDFN
                     TESAITKDGEPGKIINGLPDWLYEEEFEATRYFVWSPDSKLLAFVKFDETEVPQFSFQ
                     LYENKNSTDDLTLYPSLVSFKYPKAGQNISKVSVCVYEERTKAIRTVQLSNVGEDFYI
                     PRIKWTNSLDQLAVFVLNRNQNQLKMFLANPKSTLSTLILQENDKYYIDYQNIDACQF
                     TSDNQYFIFLSEKDGFRHVYQHRINGTPVKQLTKGNFDVTQVYGFDEKNQTLYYQSAE
                     SSPLQRDIYSVSLKGVKKKLTNGNGFHNAIFNSTFSFFVDNFSNLQTPDKLILYSNNG
                     NFIREMQNSDGILENWNTLHLPKKEFFSFTTSENISLNGWMLKPLNFDPNKKYPLLLI
                     QYSGPNSQMVLDKWVVDWEYYLAENNLVVACVDGRGTGARGVEFRKCTYEQLGVLEAK
                     DQTEAARFFSKQDYIDPQRIGIWGWSYGGFLTLMAMSSGENVFKAGIAVAPVTDWRFY
                     NAAYTERYMRRPQENFKGYDLASPIMKADRLKGELLIIHGTADDNVHAQNTLMYVKRL
                     VEADKQYNMQLFTDKNHSILGKQTRRYLYRSMSNFLFKNL"
     gene            7553..8908
                     /locus_tag="PLM04_05220"
     CDS             7553..8908
                     /locus_tag="PLM04_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02509.1"
                     /translation="MNAEKKSGNLVAIITMFFLFAMISFVTNLAAPIGVIWKNQPAFE
                     GSNFLGMLGNMMNFLAYLFMGIPAGKLLSKIGYKKTALVAIAVGFLGVLTQYLSGIIG
                     VGAELFSLPANFFVYLLGAFIAGFSVCMLNTVVNPMLNLLGGGGNKGNQLIQIGATFN
                     SLAATITPMFVGALIGDITKERILSGEVTINNVNPVLYIALGVFALAFLILLFIPIAD
                     PDENRKKRSEIKYEHSPWNFRHFVLGSIAIFIYVGVEVGIPGTLNFFLADTTIKGASL
                     DPQKAAAIAGFVAGTYWFLMLIGRFLGSLVGGKVSSKTMLTFVSALGIALVLLAILLP
                     KDITTSMPVFTGSSFSMVQVPLIALFLVLCGLCTSIMWGSIFNLAVEGLGKYTAAASG
                     IFMMMVVGGGILPLLQNWIADISTYQASYIVILVGLLYILFYALIGSKNVNKDIPVEV
                     E"
     gene            8931..9884
                     /locus_tag="PLM04_05225"
     CDS             8931..9884
                     /locus_tag="PLM04_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HON02510.1"
                     /translation="MEKPYVIGIDMGGTNTVFGIVDVRGNVISKSAIKTAVHDDVNLY
                     VNDIYHELMKLIDAEGGIDQIRGIGVGAPNGNYYSGNIEYAPNLPWKGVIPFAKLLNE
                     KFGVPVALTNDANAAAIGEMAYGIARGMKDFIMITLGTGVGSGIVIDGKLVYGHDGFA
                     GELGHTTSIRNGRLCGCGKNGCLEAYSSATGVARTAREILETTNQDSILRNIPLADIT
                     SKDVYDAAKEGDQVAIDIFNFTGKILGEAFADFVAFSSPEAIILFGGLSKAGEFILNP
                     IIENMEKNLLPIWKGKIKILFSELKESDAAVLGASALAWEL"
     gene            complement(9960..10121)
                     /locus_tag="PLM04_05230"
     CDS             complement(9960..10121)
                     /locus_tag="PLM04_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02511.1"
                     /translation="MKKVLLFCAVIFAFAACAPKESKEASTTEDSVVVVDTVVVDSMV
                     VDTTAAPMK"
     gene            complement(10329..12794)
                     /locus_tag="PLM04_05235"
     CDS             complement(10329..12794)
                     /locus_tag="PLM04_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HON02512.1"
                     /translation="MIYPKHFEQKIDFITIRQLLKDKCVSTLGIEKVDEIRFSSNFDE
                     IFHWLSETNEMLKVLTTEVQELPVNHFYDLRSSLSKIRIEGLYLTETEIFQLYQTLEN
                     VLTITNYISQLNPQLYPCLHQLTGNVNVFKPIIQRVDAILSKFGKIKDQASPQLTQIR
                     KNMTEVQNNISKSIQEILRKAQTNGYVEKDTTPALREGRLVIPVPQAFKRKINGIIHD
                     ESASGKTVYVEPAEIVEMNNRLRELEREERRETIRILTLFTDFVRPYLPDIQESQNFL
                     GKIDFLRAKALLAIDIHAIQPQLENHCLIDWHKAIHPLLFLSFSKQNKQVVPLDIQLN
                     EKQRILIISGPNAGGKSVCLKTTALLQYMLQCGLLIPLHESSRTGIFEHLFIDIGDEQ
                     SIEDDLSTYSSHLLNMKFFIRYSNDKTLLLIDEFGSGTEPQLGGAIAEAVLDRINRNK
                     AFGVITTHYTNLKQFAEETEGIVNGAMLFDRQKLQPLFQLQIGMPGSSFAIEIARKIG
                     LPEDIIDQASKKIGKDYLNYDRYVQDIIRDKRYWQNKREQIKLKEKQMEETIRRYETE
                     LTELKRQRTEIIQKAKEEAQNILEETNAKIENTIRQIKEAQAEKEKTKEIRKELENFK
                     QTVNQSVDNRLSVNPTAVSTKKKLVKKVTPKEEIHKKDVQEEKISVGSNVRLKNQGTT
                     GTVLEINGNEAIVVFGQMKSTVPFSKLEPLSRTKLKKENQNKKNIHVNTIEAVRQRKL
                     EFKTEIDVRGMRSNEALQAITYYIDDAIMVGVPSVRILHGTGTGALRQIIREYLRTIP
                     AVRNFHDEHIQFGGSGITVVELE"
     gene            13106..14491
                     /locus_tag="PLM04_05240"
     CDS             13106..14491
                     /locus_tag="PLM04_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02513.1"
                     /translation="MKIQINIKLKENRWLLMAIVIGSFFNSFMSSSLNIAMPLIDNEY
                     HVGLAKVAWISESFLLASAVGLMLAGWLADQFGRRKTFLFGNLIFGFISLFIYFVPGF
                     YPAVMLRFIQGLGSALIMASAPALIASAFDPSRRGFVLGIQTTFTYVGLSLAPLLGGA
                     LTQIFGWRSLFLFNFIFGLAALSIMFFGVKREWKAETRNKFDVGGYVIFLLAIVALVL
                     ALDWMNKFSIIMLLFSLMAGYVFIRYELKKEMPFISIAFLKSNKYFTNSNFAALINYA
                     TTFAISYVLSLYLQNVRGMTPVGAGLILCFQPLMMALFSAYAGHLSDRHNPGKIASYG
                     MAMIALALFIIGITLSEKLPLAIIAGLLAFLGFGFALFSSPNTNAVMSSADAGSYGFA
                     SAFLAFNRLFGQFISMALASAFILLSNHTRLATVEEQALLMASKIIFIFFGLLSCLGV
                     YLSYQRIRMKK"
     gene            complement(14557..20445)
                     /locus_tag="PLM04_05245"
     CDS             complement(14557..20445)
                     /locus_tag="PLM04_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HON02514.1"
                     /translation="MKKLSFILLAVVFVVVAFAAFQQPTVSFDERWKKVEELAEKQLP
                     ESALKEVDAILKDAKKQNNSPEIIKATLYKMRFTLEKDPDQAPQLIKEFETFATSTSD
                     TAEKSLLYSMTAELYAQYFQQKAYSIRQRTEISDYVPENIDEWTKNIFFDKVSQLLAA
                     SLEPAEMLQKTDIHKFETILEKGDSSHRVQPTLFDFLAYRRIDILQSLEGAQSIKNPL
                     KSEDLFSDLSHFVSLLLDTTYSKSVENQVLQTYQQLLSFHLKDNNVPALLYADLQRLT
                     YLKENSQLSNSDELYLSALARLSEQYANNENVVEVMAAMAQYYLDKEYELEENMNFAK
                     KAYAICSEGIRRFPDYPRIALLKNIQQQITQKSVSLRHSDVVRSKSQLSLYISTKNVE
                     DLQMQIYRVNASSLDYFSFTKNPRNSGKDFPDRTLWQTSEIKIKPDTNFVAVDTVLKI
                     PSGTFGIYEVQLMIKGDTSSEVARSSYVVSDLSFMVRQNEPKKTALYVLNSETGWQQK
                     EVKVSLYEQKWTGNFYQMNLLQTAVTDKNGFCTFPYNNQGTKWIFLEKGEDRYFSSSV
                     NTYYWERDTNKEKPVQLEFFTDRAVYRPGQTVYFKGIAYYADAKRQEVAKSLKFDVSL
                     IDTHYQKIASKTFTTNKFGSFAGEFVLPADGLNGTFRLESGNFSKTIWVEEYKRPTFE
                     VKIQKPTDEVKFGEKVTLTGNVKAYSGYNIADAKVNFRVVRRPHWYCSWIRSEEKAIS
                     NGETKTDANGNFTLFFIPEKSKENFSFRKDAYYVYTVYAEVTDTKGETQQGQQFVAVG
                     DKSLFILADVPEKWNKKEKADFPLRLETINGEKVDGTVQFTVYRFENSEDFATNEKWA
                     EMEEEGEEEISMKSAKKIMEGTFNTKDGKLKLDVNKWESGGYRIVFSTLDQSGKKVEL
                     GKNFILYGKNDKRPSIQTMTWLPVEEITCEVGETAEVVFGSSADNVAVLYEMMQGNTV
                     LESKWIRMNNEIKTFPIPFKAEYGDGITVMFTFMKKGLFFTESVIIRRKIAEKKLTPT
                     LTVFRNKLLPGEKAEWTVNIPEIKQIKKQAELLMDMYDASLDVIRPNEWTFQPIFRGN
                     FMESPRWDAPYNRIISDNIDIPVKYIPVEEYRLDRLNWFGLDLMAGQKYNGPFVVRGL
                     KSLSATSEFREISAMNEKFEEETVVAFSVVKTVKTDLTNVIMQPETSPVIRKNFNETA
                     FFYPQLYTDSAGDVKFSFTAPESLTRWNIKMLAHTPDLYFGQADAQAITQQDLMIQMN
                     LPRFVRCSDTLILSCIITNLSEKEISPVAHFELLDAANEQPVAVFEDSKVQLSPNETK
                     TVRWQLKDFSSHELLICKVMATAGSFSDGEQNYLPVLPDKILVTESLPMTVRGHQTKT
                     FQFESLQKKFSQVETKNLTLEFAAQPVWYAIQALPTLAEPENQNAVDYFAAYYVSTLA
                     AYIVQSYPEIEKIFRRWEKSETGKNALLSNLEKNSELKNFLLKETPWLMAAQDEMEQK
                     NRLALLFDLNMQQDKSQQYLDKLLKLQLSTGAFTWFEGMTENRYITQLVLLNMKRLHE
                     KTGLPMDKRLESAMTLALNYLDKEIVNDFEQLKKNRKNFEKENCISPLQLQYLYLRSS
                     YSEIPVPDFSQEAVKFYRHQAEKFWTDFSLYGKSQAAIVAYRDGKMALARNILKSLKE
                     NALKSEEMGMYWANNKAGYFWNERPVAVQTAIMEAFSEIGADTSDLEEMKTWLLKQKQ
                     TQRWDSPISTVDAVFALLTIGNNWLQNNDKVIIKIGDKELQPSSIEAGSGYFKQSLPV
                     EQITSASSRITVSKNSDGIGWGAMYWQYLQKMDQVQSTVGALNISKKLFVEKVTSAGR
                     TLVPIEQVKISLGDKVVTRLVVTTDRNLEFVALKDIRAAGLEPVNQISGCQWKQGVCY
                     YQTIKDASAEFFFSSLPKGTYVFEYALWANNVGTFNGGTASIQCQYAPEFVGYAAGQQ
                     VVVQ"
BASE COUNT         6125 a         3875 c         3965 g         6756 t
ORIGIN      
        1 tatcaactcg gcacataccg gaactcgcat cctgcaagct taataccata catacaaaaa
       61 aaaccggtag aaaatttcac cggttttttt gtaaaaaata tttttcagat ttatcttccc
      121 gtttcgttca atatcgaagt tttctttttc aaaaatttca ataccagata aaggatgcct
      181 gctgcaaatg ttccccaagc tatccagttc aaatcaatac ggatatgatt cgttagaaaa
      241 gaataattga tattgtgaaa tacaaacagg aggacaaata cagccagcaa gccgtttttt
      301 tcttttttga gtactttctt ccagctgaaa ggatattttg aaggaatgta ttttttaaag
      361 cttggaataa aagccggcgt tttgttggcc caatcggtat aaacagaacc aaattttcga
      421 cgcagaaact gttcttcggc aaacatgatt ctttcgtaat aaatccagta aagaagaatg
      481 aaagccacga taaaccagcc atttttcgtc aggacagcag caccaagcca catgaaaaaa
      541 ttacccaaat agagtggatg acgaaccata gaataaattc cggtggtatt caattcatcc
      601 gccaactgtt ctttggtgtt tcgtcccgat gtatttgccg gcgtatgtcc cactgcataa
      661 gctcttatga aaagtccgaa aagtgaaata aacaaaccga taaattcaac ggtcgaatat
      721 ttcaatacaa aaaaatgtat gaccgtcatg tggttcaata aaaaccgcaa gccctactgc
      781 tacaataatc aaaggtaaat atccacgata cttgaaaagg aaattaccct gtttctccat
      841 ttcttcctgc aatgccataa aatattattt aaatgatttt tgtatagttg attaattata
      901 gaaaagttta attgttattg ttttgattta ctttttttga gaggatttac cattctattt
      961 cgctcatacc atgctctttc aggtattgat tggcgagttg aaaatgtcga ttcccgataa
     1021 atccccgata agccgaaaga ggagaaggat gaggcgattt cagcaccaaa tgtttattgg
     1081 tgtcgatata taggcctttt tgttgggcat aacttcccca aagtaaaaac accaaatgtt
     1141 cctttttttc ggccaatatc cgaattactg cgtcggtaaa tgtttcccaa ccttttcctt
     1201 gatgagaacc ggccaaatga gcctgcacgg tgagaatggc attgagcagc aacacgccct
     1261 gttttgccca agagcttaaa tcgccatttt tgggcattgg tttggcaaat tcattttcta
     1321 tttccttata aatattgacc agcgaaggcg gaaaatccac gccctcgttt accgaaaaac
     1381 ataaaccgtt agcctgaccg ggaccatgat acggatcctg acctataata actactttca
     1441 ctttatcata cggacacaga ttgaaagcat taaaaataag agagcccggc gggaaaattt
     1501 tttttgtttt atattccttt cgtacaaagt cggtaagctg tttaaaatac tctttttcaa
     1561 attccgattg aagcacctcc ttccagctcg attctatttt gacgtccatt agaaaaggtt
     1621 ttttatatcg ctgtgaaaaa taaagttttc cggtgcaaat ttacatcaaa aaaaatattt
     1681 atgctgctac gaaagttcaa tgatctgttt gtttttttaa tttataactt tttctttagc
     1741 agtttatttt caatggatta aaaatttttt ccattggaca attcaaaaaa agaaattaat
     1801 tcaaagccga tgtttatatt ttactgataa taaacaattt gattttacgt tgaataaaga
     1861 aattttaatg tttgcttcca tgtaaaattt cattttatat ttcagtcttg cattaaaaaa
     1921 atcaatttta gctaaaaaaa tagcgcaaat gttttttaat cagaataaaa gcaatatatt
     1981 tgcaatccta tttcaattca ataaatagtt ttcttaattt attaacatat ttaaaatatt
     2041 agcaaaaatg actaaagcag aaattgtaaa tgaaattgcc aaaaaaacag ggatcgataa
     2101 agctactgtt ttggcatctg ttgaagcttt tatggatgca ataaaagatt ctctttctaa
     2161 aaatgagaat gtatatttaa gaggttttgg tagttttgtg gtaaaaacca gagcacagaa
     2221 aactgcccga aacatttcaa aaaacacaac cattatcatt ccacctcaca acattccttc
     2281 tttcaaacct gcaaagaaat ttatgtctgt agtgaagtaa attatttcat ttcaaaaatt
     2341 aaaattttaa aaaatgccaa gcgggaaaaa aagaaaaaga cacaaaatgt ctacgcacaa
     2401 gcgtaaaaag agactaagaa agaacagaca caagaaaaaa taggtcgagg ggtctttaaa
     2461 tgattaaagt tcaaggaaac aaactttaaa gcattcattg aggctttgag tttgtttttt
     2521 tgttgaaaat cgaataacca aaatcaataa aaaagtgaat agcgaattag tagttgatgt
     2581 aaagccatca gaaatttcta ttgcgctgct cgaagataaa cgtctggttg aacttgccca
     2641 agaaacacga gaagaacaat tcgctgtcgg gaatatttac ttaggaaaag taaaaaaaat
     2701 tatgccaggc ttaaatgcag cctttgttga tgtcggttac gaaaaagatg cttttattca
     2761 ttatcttgat ttaggtccca atttcaatac cctttctcag tttactttgc aggcgctcag
     2821 cgacccgaaa aaagcgccct cgatgcaaaa agttaaaatg cttccggaaa ttgaaaaatc
     2881 aggttttatc aacgacctgc tcaagccggg tcaacaaatt ctggtgcagg tggccaaaga
     2941 acccatctcc accaaaggac cgcggctgac ggccgaaatt tctttagccg gacgattttt
     3001 ggtgttaatt ccctttaacg acaaggtgct tatttctcag aaaattaaat ctgaaaatga
     3061 gcgtgtccgt cttaaacagc ttattcagag catcaaaccg aaagggtttg gcgttattgt
     3121 tcgtacagtg gcagaagaca aaaaagttgc cgaactgatc aatgaactca atgtgctcgt
     3181 aaaacgctgg gatgaagctg ttgccaaact tcaacaagca aagggagtta ctcttatttc
     3241 agaagaattg ggtcgtactg taggaattat cagggattta tttaacccga gttttaaaaa
     3301 catttatgtg aacgatccca atgtatttgc agaagtgcaa gactacattg aatggattgc
     3361 tccggaacaa aaaaatatcg taaagcttta taaagatgaa actccgatat ttgatcattt
     3421 tggcattacc aaacaaataa aatcgtcttt tggtaaaacc gtttcattta aaaatggagc
     3481 ttatctgatt attgaacata cggaagcttt acacgttatt gatgtgaaca gtggcaatcg
     3541 ggcaaagaca ggaaacggac aagaagccaa cgctttggaa gttaacttgg ctgctgccga
     3601 cgaaatagcc cgacagctcc gtttgcgcga tatgggcgga attattgtag tggatttcat
     3661 tgatttgcat gaatctgaca atcgtcagaa agtgtatgac cggatgaaag agatgatggc
     3721 aaacgacaga gccaaacaca atattttgcc tctcagcaag ttcggactga tgcaaataac
     3781 acgtcaacgt gttcgacccg atacgcatgt cgatattgag gaagcttgcc ctacttgttt
     3841 cggaacaggg aaaataaaac cttctatttt atttactgac ctattggaaa gcaaaataga
     3901 ttatttggtc aatactttaa aagttaaaga atttactctc tatgtacatc catacgttga
     3961 agcctatatt tccaaaggaa tgattccttt gaaaaacaaa tggaaaatta aattcggacg
     4021 aggatgtaaa gtgagagctt cacaggaatt gggtttcctg cagtataaat ttatcgataa
     4081 taaagggatg gaattgaatc ttcaagaaga aatagaaaaa atttcgtaat tggacatttg
     4141 taaaaaaatt ctaacagaaa actgcctttt ggatgttacc atctaaaggc agttttttat
     4201 ttcggtatgc taaattccat tatttatcgc cgggttattt tctataaaag tgtgagtgca
     4261 ttggtagcct tcttcataaa tttgctggta agcatcaaaa ctgaattcgt gaaattttcg
     4321 gatcgctttc ggttcaatca aatattggca aagttctctc cctttttgtg cgtttttatg
     4381 ttgtatgatg cggatggaat aagtcaaggt gtcaatcggt ttttttaatt gaacctcatt
     4441 caggtgagga atcacatcta cgccgataat cgtactacaa ttctctttga gagcttgtgc
     4501 cggcatgttg ttcaataatc cgccatccac ataatattct ttttttacct gaatggcttc
     4561 aaatacgccc ggaatacttg ccgaagcggc tacccattta tccagttcct tgccgctttc
     4621 aattatttcc cattcggctt tattcaaatt gctgacgcag acagacattt ttttctttaa
     4681 taattcgaaa ctgttgtgag ggatcaattc ctttatcaaa tcccgcaaca ttttgtgggt
     4741 ggatagtccc gatttcaacg acatgggatg gatagacatt aatttggtta ttttgtacaa
     4801 tttgtcttct ctgatcattt gcagcatttg ggccggtgta aacccagcag cataaagcgt
     4861 tcctacaatg gctcccatac ttgagcctgc aatctgaacg gggaaaatgc cattttcttc
     4921 aagtgcctgt aaaacgccaa tatgggcaaa gcctaaagcg ccgcctccac tcaaacaaat
     4981 gcctgttttg ttaatattct gttcatggtt tgtgatgttg tcatcgttca ttgttatatg
     5041 tatttttgta ttgttgtgga tgttctttct tgtataatgt taaaccattc aaaaaaattg
     5101 acctaaaatt tttaaaactg atgaattacg ctacttttgt gtgaatttac aagaaaaaaa
     5161 tcaactaata ggtaatttaa cggttaaaag ttttttatga aacgtatttt tttagctttt
     5221 gttttggcat ttgcagtgca attttcgttt gccgacttgc tggacgatat tgttgatggc
     5281 aagtttaatg tgaataatat catggttccc cgctcgatga aagatggaga gaattatacg
     5341 ctgatgaaag ataatgcagt gctaaaatac aattacaaaa ccggtcaatt ggtagatacc
     5401 atttttagta ttcatcgctt gaaagatgcc ccattcaagg aaattaatgg ttatgaattc
     5461 agtccgaatg aagccaaact attggtttat aacaatgtaa aaaaacgata cagacgtact
     5521 ttcacagccg attattatgt ttacgacatt aaaaggaacg aaattcagcc gctttcggat
     5581 aacggctcgc aggaagtacc tcttttttct cccgacagta gatatattgc tttcggaagg
     5641 aacaacaatc tttatttgaa aaaactcgat tttaataccg aatcggctat aacaaaagat
     5701 ggtgaaccgg gaaaaattat caatggtttg cctgactggc tgtatgaaga agaatttgaa
     5761 gcaacgcgtt attttgtttg gagccccgac agcaaattat tggctttcgt aaaatttgac
     5821 gaaactgaag tgcctcaatt ttcatttcaa ttgtatgaaa ataaaaactc tacggacgat
     5881 ttaacgcttt atccatcact tgtttctttt aaatatccca aagccggaca aaatatttcc
     5941 aaagtaagtg tttgcgttta tgaggaacgg acaaaagcga tcagaactgt tcagttgagt
     6001 aatgttggag aagattttta tattccgagg ataaaatgga ccaattcact ggatcagttg
     6061 gcagtttttg tgttgaatcg caatcaaaat cagttgaaaa tgtttcttgc caatccaaaa
     6121 tctactctgt ccactctgat attgcaggaa aacgataaat attatattga ttatcaaaat
     6181 attgatgctt gccaatttac ttccgacaat caatatttta tttttctgag tgagaaagat
     6241 ggctttcgtc atgtttatca gcatagaatc aatggcacgc cggtcaaaca actgacaaaa
     6301 ggaaattttg atgtaacgca agtttatggt tttgacgaaa aaaatcaaac gctttattat
     6361 caatcggcag aaagttctcc tttgcaaaga gatatttatt ctgtcagctt aaaaggcgtt
     6421 aaaaagaaac ttaccaatgg gaatggtttt cacaacgcca tttttaattc gaccttcagc
     6481 ttttttgtcg ataatttttc caatctacaa actcccgata aacttatttt gtatagtaac
     6541 aatggcaatt tcattcgtga aatgcaaaac tctgacggca tattggaaaa ttggaatacc
     6601 cttcatttgc ccaaaaagga atttttcagc tttacgactt ctgaaaatat ttcgttaaat
     6661 ggctggatgc tcaaaccgtt gaattttgat ccgaataaaa aatatccatt attgttgatt
     6721 caatatagtg ggcctaattc gcaaatggtt ttagacaagt gggttgtgga ttgggaatat
     6781 tatttggctg aaaataacct ggtggtggct tgcgttgatg gacgtggcac tggagctcgc
     6841 ggagtagaat tcagaaaatg tacgtatgaa cagttaggcg ttctcgaagc aaaagaccag
     6901 acagaggcag cccgtttttt cagcaagcaa gattatattg atccgcaaag aatcggtatt
     6961 tggggttgga gttacggcgg atttcttacg ttaatggcca tgagtagtgg agaaaatgta
     7021 tttaaggcag gcattgctgt agctccggta accgactgga gattttacaa tgctgcatac
     7081 accgaaagat acatgcgtcg tcctcaggaa aattttaagg gatatgatct tgcttcgccc
     7141 ataatgaaag cagatagatt aaagggtgaa ttgctgatca ttcatggtac ggccgatgat
     7201 aatgttcacg cacaaaatac cctgatgtat gtaaaacgat tggtagaagc tgacaaacaa
     7261 tataacatgc agctgtttac ggacaaaaat cacagcattt tgggaaaaca aacgcgtagg
     7321 tatttgtatc gttcgatgag taattttttg tttaagaatt tgtaattcca ttacatataa
     7381 aaaatcaaac tgtttttatt ggttaaaaat atgttgtaaa acattgtttg gataatgctt
     7441 ttttgtttat tctgttaaaa ggaactgaca aaaaaatggt atttttgtcg gtcaataaaa
     7501 attaattcaa aaaacatttt cattacaatt aactttatta attctaaaca atatgaatgc
     7561 agagaaaaaa agtggaaatc tggtggccat tatcactatg tttttcctgt ttgcaatgat
     7621 ttcgtttgtt actaatcttg ctgcgccgat tggggtaatt tggaaaaatc agcctgcttt
     7681 tgaaggatcg aatttccttg ggatgcttgg aaatatgatg aactttttgg cttatctgtt
     7741 tatgggtatt ccagccggta aactgctttc aaaaattggt tataaaaaaa cggctcttgt
     7801 tgcaattgca gtaggttttt taggtgttct gacacagtat ctgtccggta ttattggtgt
     7861 tggagctgaa cttttctcac tacctgccaa tttctttgtt tatttgttgg gtgcatttat
     7921 agccggtttc tctgtttgta tgcttaacac agttgtcaac ccaatgttga atttacttgg
     7981 aggcggtggc aacaaaggaa atcagttgat tcaaatcgga gcaactttca attctttggc
     8041 tgcaaccatt acgcctatgt ttgttggagc tttgattggt gatataacca aagaacgcat
     8101 tttgtcaggt gaagttacca ttaacaacgt aaatcctgtg ctttatattg ctttgggcgt
     8161 ttttgcactt gcctttctta ttcttttgtt tattcctatt gcagacccgg atgaaaacag
     8221 aaagaaaagg tccgaaatca aatacgaaca ttcaccgtgg aatttccgtc attttgtact
     8281 gggatccatt gccatattta tttatgtagg agtagaagtg ggcatacccg gaacgttaaa
     8341 tttcttcctt gccgatacaa caattaaagg agcttcttta gatcctcaaa aagcggctgc
     8401 cattgccggt tttgtggcag gcacttattg gtttctcatg cttattggac gttttcttgg
     8461 tagtttggtt ggtggaaaag tttctagtaa aaccatgctt acatttgttt ccgcattagg
     8521 aattgcattg gtgttgcttg ccattttatt gccgaaagat attactactt ccatgcctgt
     8581 atttaccggt tcatcattca gcatggtgca agtaccgctc attgctttat tccttgttct
     8641 ttgcggcttg tgtacttcca ttatgtgggg tagtattttc aacctcgcag tagaaggact
     8701 tggaaaatat acggctgctg cttcaggaat atttatgatg atggtagtag gtggtggcat
     8761 tttaccactt ctgcaaaact ggattgccga catttctact tatcaggcaa gctacattgt
     8821 aattttggtt ggattgctgt atattttgtt ctatgcttta attggttcta aaaatgttaa
     8881 taaagatatt cccgttgaag tggaataaat ttcaataaaa ttatcgtatt atggaaaaac
     8941 cttatgttat cggaatagat atgggcggaa ccaataccgt tttcggtatt gtggacgttc
     9001 gtggaaatgt gatttctaaa agtgccatca agactgcagt tcacgatgat gtcaatcttt
     9061 atgtaaatga tatttaccat gaattaatga aattgattga tgctgaaggc ggtatagatc
     9121 aaataagagg gattggggtt ggagctccca atggaaatta ttattcaggc aacatcgaat
     9181 atgctcccaa tttgccatgg aaaggagtaa ttccttttgc aaaactgctt aacgaaaaat
     9241 ttggcgttcc agtggctttg actaatgatg ccaatgctgc agccatcggc gaaatggctt
     9301 atggaattgc tcgcggcatg aaagatttta tcatgattac attgggaacc ggcgttggaa
     9361 gtggaattgt tattgacggg aaactggttt acggccatga tggatttgca ggtgaattgg
     9421 gtcatacaac ctccatccgc aatgggcgtt tatgtggctg tggaaaaaat ggttgtttgg
     9481 aagcctattc ttctgctacc ggtgtggcac gcacagctcg tgaaattctt gaaactacca
     9541 atcaagacag tatcttgcgt aatattcctc ttgccgatat tacttcaaaa gatgtttatg
     9601 atgctgctaa ggaaggagat caggtggcaa tagacatttt taattttacc ggaaaaattt
     9661 taggtgaagc atttgccgat tttgttgctt tcagttctcc ggaagccatt attctttttg
     9721 gtggtttatc aaaagcggga gagtttattt taaatcctat tatagaaaat atggaaaaaa
     9781 atcttcttcc tatttggaaa ggtaaaatca aaatcttgtt ttccgaactg aaagaatcgg
     9841 atgcagctgt attgggtgcc agtgcattag cttgggaact ttaaaataac ttaaaaaata
     9901 atcgagtagg aagaaaatga aaatctcacc tactcgatta caaaaaaaaa acggcatttt
     9961 tacttcattg gagcagcggt agtgtcaacc accatactgt caacaacaac agtatcaaca
    10021 acaactacag aatcttctgt ggttgaggct tctttgcttt ctttaggagc acaagctgca
    10081 aatgcaaaaa taacagcaca aaacaacaat acttttttca tttttcaaaa ataatttaaa
    10141 aatttaacat gtgaacttct atgacgaaaa tttctgcaaa gttatgaata ttttaaatat
    10201 attgttatca aaataatttt ttttttcgtt ttttttcaga ataatttcag cattcaattt
    10261 tttaattcca tactgtttgt tttaatttat cttgttttta cctaaaaaaa ttaaaagaaa
    10321 ttttattttt attccaactc cactactgta atacccgagc caccgaattg gatatgttcg
    10381 tcgtgaaagt ttcgtacagc cgggatagtg cgtaaatact cccgaataat ttggcgcaaa
    10441 gcacctgttc cggtaccatg aagaatgcgt accgaaggga ctccaaccat aatggcgtcg
    10501 tcgatatagt aggtaatggc ttgcaaagct tcgttactac gcatgccgcg tacatcgatt
    10561 tcggttttga actcgagttt gcgttgccga acggcttcga tggtattgac atggatattt
    10621 tttttgtttt gattttcctt tttaagtttg gtgcggctca gtggttcgag ttttgagaac
    10681 ggcaccgtag atttcatttg cccgaaaacg acaatagcct catttccatt aatttcgaga
    10741 acagttcctg tagtaccttg atttttcagc ctgacgttgc tgccaacaga aattttttct
    10801 tcctgtacat cttttttatg gatttcttct ttgggtgtta ctttttttac aagtttcttt
    10861 ttagttgaaa ctgcagtggg atttacgctt aatcgattat caacagactg attgacagtt
    10921 tgtttaaaat tttccagttc tttcctgatt tctttggttt tttctttttc tgcttgtgct
    10981 tcttttattt gtcgaatggt attttcaatt ttggcatttg tctcttcgag aatattttgg
    11041 gcttcttctt tagctttttg tatgatttcc gtccgctgcc gtttcagttc agtcaattcg
    11101 gtttcgtaac ggcggatagt ttcctccatt tgtttttctt tcagtttgat ttgttctctt
    11161 ttgttttgcc aataacgctt gtcgcgaata atgtcctgaa cataacggtc ataattgaga
    11221 taatcttttc cgattttttt tgatgcttga tcgataatat cttcgggcaa tcctattttt
    11281 ctcgctattt caatggcaaa cgagctaccc ggcataccaa tttgaagctg gaaaagcggt
    11341 tgaagttttt gtcggtcgaa gagcatcgct ccgttgacta ttccttccgt ttcttcagca
    11401 aattgtttta aattggtgta atgagtggtg attacgccga aagctttgtt gcgattgata
    11461 cggtccagta cggcttcagc tattgcgcca cccaattgag gttcggttcc ggaaccgaat
    11521 tcatcaatca gcagtaatgt tttgtcgttg ctgtagcgaa taaaaaattt catgttcaac
    11581 aaatgtgaac tgtaggtgga caagtcgtct tcaatggatt gttcgtcgcc aatatcgata
    11641 aaaagatgtt caaaaattcc agttctactg ctttcatgta gcgggatcag caagccgcat
    11701 tgcagcatgt attgcaacaa cgctgtagtt ttcagacaaa cggattttcc tcctgcattg
    11761 ggacctgaaa taattagaat acgctgtttc tcattcagtt gaatatccag cggaacaact
    11821 tgtttgtttt gtttggagaa tgaaagaaaa agcaggggat gaatggcttt atgccagtcg
    11881 atgaggcaat gattttccaa ttgcggttgt atggcatgga tatcaatagc cagtaatgct
    11941 ttggcacgca aaaaatctat ttttcccaaa aaattctgtg attcttgaat gtcaggcaaa
    12001 tagggacgta caaaatcggt aaatagggtc aaaattcgga ttgtttcgcg gcgttcttct
    12061 ctttcgagtt cacgcaaccg gttattcatc tccactattt cggccggttc cacataaacg
    12121 gtttttccgc ttgccgattc atcgtggata atgccgttga tttttctttt aaatgcctgt
    12181 ggtacaggta ttaccaacct cccttcacgt agagcgggag tggtgtcttt ctctacatac
    12241 ccgttggtct gcgctttacg caaaatttcc tgaatagatt ttgagatatt gttctgaact
    12301 tcggtcatgt ttttgcgtat ctgcgtcaac tgaggagatg cctgatcctt gatttttcca
    12361 aatttggaaa gaatggcgtc gacacgttgt atgatgggtt tgaaaacatt tacgttgccg
    12421 gtcagttgat gaagacaagg ataaagctgc ggattcagtt gtgaaatata attggtgatg
    12481 gtcagcacgt tttccagcgt ctgatacaac tgaaatattt ctgtttcggt aagataaaga
    12541 ccttcgatac gaattttgga aagagaagaa cgcaaatcgt aaaaatgatt tacaggcaat
    12601 tcttgcactt cagtggtcaa cactttcaac atttcattgg tttccgacag ccaatgaaag
    12661 atttcatcaa agttggaaga aaaacgaatt tcatcaactt tttcgattcc caacgtgctg
    12721 acacatttat ctttcagcag ttgccgaatg gtaatgaagt cgattttctg ctcgaagtgc
    12781 ttaggataaa tcattgagca tgtaattaaa ttttttaaaa tcaaatagct attaaaaact
    12841 tggtttgctg cgtattgaga aagataagat aaactctatt tttttaaaaa cagttttgtc
    12901 caaaaaatat tataaatttg ctagcctttt tctttaaacc acccatgttt atcaccttac
    12961 ttttgtaaag taaataaatc gaatgattga tttttcatat tatgttgttt gcagactgaa
    13021 cccctctgtg ggaagacaaa attatgaaaa atatttcatt aagccgtttt aaaaagtatt
    13081 ttagtaaatt gtataagcaa acggtatgaa aatacaaatc aatataaaat tgaaagaaaa
    13141 ccgatggtta ttgatggcca ttgtgatagg ttcatttttt aacagtttca tgtcctcatc
    13201 gctgaatatc gccatgcccc ttatcgacaa cgaataccat gtaggactgg ccaaagtagc
    13261 atggatatcg gaatcttttc tgttggcttc tgccgtcgga ttaatgttgg ccggttggct
    13321 ggccgatcag tttgggcgac gcaaaacttt tctttttgga aatttgattt tcggttttat
    13381 ttcacttttc atttactttg ttcccggttt ttacccggct gtaatgttgc gatttataca
    13441 aggattgggg agcgcattga taatggcctc cgctcctgcc ctgattgctt ctgctttcga
    13501 cccttccaga agaggatttg tactgggaat tcaaaccacc tttacttacg tcggactttc
    13561 actggctccg cttctgggtg gcgctttgac tcaaattttc gggtggagaa gtctgtttct
    13621 tttcaacttt atttttggac tggcggcttt gtccattatg ttttttggtg tgaaaagaga
    13681 atggaaagcc gaaacaagaa acaaatttga tgttggcgga tatgttattt ttctcttggc
    13741 catagttgca ttggtacttg cacttgactg gatgaacaaa ttttcaatta tcatgctgtt
    13801 attcagtctt atggcaggtt atgtttttat tcgctatgaa ctgaagaaag aaatgccatt
    13861 tatttcaatc gcttttctga aatcgaacaa atattttacc aattccaatt tcgcagcttt
    13921 aatcaattac gccaccacat ttgccattag ttatgtgctg agtttgtacc tgcaaaatgt
    13981 acgcggaatg acacctgtcg gcgccggtct tattttatgc tttcaaccac tgatgatggc
    14041 tttgttttca gcctatgcag gtcatttgtc ggacagacac aatcccggca aaatagcatc
    14101 ttacgggatg gctatgatcg ctttggcatt gtttatcatt ggaattactc tgagcgaaaa
    14161 acttccgctg gcaattatcg ccggattgtt ggcatttttg ggatttggat ttgctctttt
    14221 ttcttcaccc aacaccaatg ccgtgatgag ctcggcagat gcgggttctt atggttttgc
    14281 ttcggctttt ttggctttca accggctgtt cggacagttc atttcaatgg cattggcatc
    14341 ggcatttatt ttgttgagta atcatacccg cttagcaacc gttgaggaac aggctttact
    14401 aatggcctca aaaataattt tcatcttttt tggcttgctt agttgtctgg gagtttatct
    14461 ttcttatcag agaattcgca tgaaaaaata gaaataccaa aacaaaatca tgcgaatttc
    14521 tttacacact caaatcatca aatttttccc gaaaagttat tgaacaacaa cctgttgtcc
    14581 tgctgcatag ccgacaaatt ccggtgcata ctgacactga atggaagctg ttccgccgtt
    14641 gaaagttccg acgttattag cccaaagggc atattcaaac acataggttc cttttggcaa
    14701 agacgaaaag aaaaattctg ccgaagcatc ttttatggtc tgataataac aaacaccctg
    14761 tttccactga cagcccgaaa tttgattcac cggttcgaga cccgccgcac gaatgtcctt
    14821 caaagcaaca aattcgagat ttctgtcggt agtaaccacc agccgcgtaa caaccttgtc
    14881 tcccagcgaa atcttaacct gttcgattgg aacaagcgtt cgaccagcgg aagtaacttt
    14941 ttccacaaac aattttttgg aaatattcaa tgcaccgacc gtactctgca cctgatccat
    15001 tttttgtaaa tattgccaat acatggctcc ccaaccaatt ccatccgagt ttttgctgac
    15061 cgtgatgcga cttgaagcag atgttatttg ctccacaggc aacgattgct tgaaatatcc
    15121 gctcccggct tcgatggatg aaggctgaag ttctttatcg ccaattttga taataacttt
    15181 atcgttgttc tgcaaccagt tatttcctat ggtaagcagg gcaaaaacag catcaactgt
    15241 tgaaatgggc gaatcccatc gttgcgtttg tttttgtttg agcaaccagg ttttcatttc
    15301 ttccaggtcg gaagtgtcag ctccaatttc cgagaaagct tccataatag ctgtttgaac
    15361 agcaacagga cgttcgttcc aaaaatatcc ggctttattg ttagcccaat acatacccat
    15421 ttcttccgat ttgagtgcat tttctttcaa agatttcagt atgttgcgag ccaaagccat
    15481 ttttccatca cgataagcaa caatggcagc ctgcgatttg ccataaagtg agaaatccgt
    15541 ccagaatttt tccgcctgat gccgataaaa ttttaccgcc tcctgtgaaa aatcgggaac
    15601 gggaatttcc gaatagctcg aacggagata caaatactga agttgaagcg gactgataca
    15661 gttttccttt tcaaaatttt tccggttttt cttcagttgc tcaaaatcat tgacaatttc
    15721 tttgtcaaga taattcaaag ccagcgtcat ggccgactcc aaccttttat ccattggcaa
    15781 accggttttt tcgtgtaacc ttttcatatt gagcaaaaca agctgcgtga tgtaacgatt
    15841 ctccgtcatg ccttcaaacc aagtgaaagc tccggtcgaa agttgaagtt tcaacaattt
    15901 gtcgagatat tgttgcgatt tgtcttgctg catattcaaa tcgaaaagta aagccaatcg
    15961 atttttctgc tccatttcat cctgagcagc catcaaccag ggtgtttcct tcagtaaaaa
    16021 gtttttcagt tcggaatttt tctccagatt cgataaaagt gcatttttcc ctgtttccga
    16081 cttttcccaa cgtcgaaata tcttttcaat ttcgggataa gattgaacaa tataggctgc
    16141 cagcgtagaa acataatagg cagcaaaata atcaacagcg ttttggtttt cgggctcggc
    16201 aagagtaggc aaagcctgaa tggcgtacca aaccggttga gcagcaaatt ccagcgtcag
    16261 atttttagtc tccacctgtg agaatttttt ctgtaaactt tcaaattgaa aagttttggt
    16321 ttgatgaccg cgtactgtca tgggtaaact ttctgttacc aaaatcttat cgggcaaaac
    16381 cggaagataa ttctgctctc catcgctgaa actgcctgct gttgccatca ccttacaaat
    16441 caacagttca tgcgaagaaa aatcttttaa ttgccaccgc acggtttttg tttcgttggg
    16501 ggataactgt actttgctgt cttcaaaaac tgcaaccggc tgttcatttg ctgcatccaa
    16561 cagttcgaaa tgtgccaccg gggaaatttc tttttccgaa aggttggtaa taatgcaact
    16621 caatatcaac gtgtccgagc aacgaacaaa acgaggcaaa ttcatctgaa tcattaaatc
    16681 ttgctgagtg atggcctgtg catcagcttg tccgaaatat aaatcgggcg tatgagccag
    16741 cattttaatg ttccatcggg tcaaactttc cggtgcagtg aaagaaaatt tcacatcgcc
    16801 tgcactgtcg gtataaagtt gcggatagaa aaatgccgtt tcattgaaat ttttccgaat
    16861 gactggagaa gtttcaggtt gcatgattac attcgtcaaa tcggttttta ctgtttttac
    16921 tactgaaaaa gcaacaacag tctcttcctc gaatttctca ttcatggccg aaatctctct
    16981 aaattcagat gttgcactta aactttttaa gcctcttaca acaaaaggac cattatactt
    17041 ttgacctgcc atcaagtcca gaccaaacca gttcaaccga tccaaccgat attcttctac
    17101 cggaatatat ttaaccggaa tatcaatatt atcggaaatt atcctgttgt aaggtgcatc
    17161 ccagcgcgga ctttccataa aatttccacg aaagataggc tgaaatgtcc attcattcgg
    17221 ccgaatgaca tccagcgaag catcgtacat gtccatcaac aattctgcct gttttttaat
    17281 ctgttttatt tcaggaatat tgactgtcca ttccgctttt tcacccggca aaagtttatt
    17341 gcgaaaaacg gttaaagttg gcgtcaactt cttctccgct atctttcggc gaatgattac
    17401 actttcagtg aaaaacagtc cttttttcat aaaggtaaac atcaccgtga tgccatctcc
    17461 atattcagct ttgaatggaa tcggaaaagt tttgatttcg ttgttcatcc tgatccattt
    17521 gctttccaaa acggtatttc cctgcatcat ttcataaagc acggcaacat tatcagccga
    17581 cgaaccaaaa acaacttcag ctgtttcacc tacttcacag gttatttctt ccacaggcaa
    17641 ccaggtcatg gtttgaatag acggacgttt gtcgtttttt ccgtaaagaa taaagttttt
    17701 acccaattcc acttttttgc ccgactgatc aagcgtagaa aaaactatac gataaccacc
    17761 cgattcccat ttatttacat ctaattttaa cttaccatct ttggtattga aagttccttc
    17821 cattatcttt tttgctgact tcatggaaat ctcttcttca ccttcttctt ccatttctgc
    17881 ccatttttca ttggttgcaa aatcttccga attttcaaat cgataaacgg taaattgtac
    17941 cgtaccatcc accttttcac cgtttatggt ttcaagccgc agcggaaaat cggctttttc
    18001 tttcttgttc catttttcgg gaacatcggc caaaataaac aacgacttgt cgccaaccgc
    18061 tacaaactgc tgaccttgtt gtgtttcgcc tttggtatcg gtaacttcgg cataaactgt
    18121 gtaaacataa taggcatctt ttctgaaaga aaagttttcc tttgattttt cgggaataaa
    18181 aaaaagtgta aaattaccgt ttgcatcagt ctttgtttct ccattcgaaa ttgctttttc
    18241 ttcgcttctg atccaactgc aataccagtg tggacgtcga actacacgaa aatttacttt
    18301 tgcatccgca atattataac ctgaataagc cttcacattg ccggttagcg taactttttc
    18361 cccaaacttt acttcatcgg tcggtttttg aatttttact tcaaaagtgg gacgtttgta
    18421 ttcctccacc caaatagttt tggaaaaatt tcccgactca agccggaaag ttccattcaa
    18481 tccatcggca ggcaagacaa attctcctgc aaatgaacca aatttattgg tagtgaatgt
    18541 ttttgaagca attttctgat aatgggtatc aataagcgaa acatcaaatt ttagcgattt
    18601 tgccacttcc tgacgcttcg catcggcata ataggcaatt cctttaaaat agactgtttg
    18661 tccgggacga tacactgcac ggtcggtgaa aaattccaac tgaaccggtt tttctttatt
    18721 tgtatctctt tcccaataat aagtatttac agaagaacta aaatatcgat cttctccttt
    18781 ttcgagaaat atccatttcg taccctgatt gttgtaagga aatgtgcaaa aaccgttttt
    18841 gtcggtaaca gcagtttgta aaaggttcat ctgataaaaa ttgcctgtcc atttttgttc
    18901 gtaaaggctc actttcactt ctttttgttg ccatccggtt tcgctattta aaacatataa
    18961 tgctgtcttt ttcggctcgt tctggcgtac cataaaactt aaatcggaaa ccacataaga
    19021 acttctggca acttcactgg aagtatcgcc ttttatcatc aactgaactt cgtaaattcc
    19081 aaaagttccc gaaggaattt ttaaaacagt atcgacggct acaaaatttg tatccggctt
    19141 aatttttatt tccgaagttt gccacaaggt acggtcagga aaatcttttc ctgaatttcg
    19201 cggatttttg gtaaatgaaa aataatcgag cgatgaagca ttgacgcggt aaatctgcat
    19261 ttgtaaatct tccacgtttt ttgtagaaat atacaatgag agttgtgatt ttgatcgaac
    19321 aacatcagaa tgccgcaaag aaacagactt ctgcgttatt tgctgctgga tatttttcaa
    19381 caatgcaata cgcggataat caggaaaacg gcgaattcct tcggaacaaa tagcgtaagc
    19441 ctttttcgca aaattcatat tttcttccaa ttcatattct ttgtcgagat aatactgtgc
    19501 cattgcagcc ataacttcca ctacattttc gttgtttgca tattgttccg ataaacgtgc
    19561 cagagccgac aaatacagct catcgctatt cgacaactgg gaattttctt tgagatatgt
    19621 taaccgctga agatcggcat acaaaagtgc cggcacatta ttatccttca agtgaaaaga
    19681 aagcaattgc tgataggttt gcaacacctg attttcaacg gatttggaat aggttgtatc
    19741 caataataac gaaacaaaat gagataggtc agaaaacagg tcttcggatt ttaacggatt
    19801 ttttatggat tgtgcacctt ccaacgattg caaaatgtcg atacggcgat aggccaaaaa
    19861 atcaaaaagt gtgggctgta cccgatggct gctatctcct ttctcgagaa tggtttcaaa
    19921 tttatgaata tcggtttttt gaagcatttc tgccggttcc aatgaagcag ccagcaactg
    19981 actgacttta tcgaagaaaa tatttttggt ccattcgtcg atattctccg gaacataatc
    20041 ggaaatttcc gttcgttgac gaatggaata ggctttttgc tgaaagtatt gagcatagag
    20101 ctctgccgtc atagaataaa gcaacgattt ttcggctgtg tcggaagtac tggttgcaaa
    20161 agtttcaaat tctttgatga gttgtggtgc ctgatcggga tctttttcca gcgtgaaacg
    20221 cattttgtaa agagtagctt tgataatttc cggagaattg ttttgttttt ttgcgtcttt
    20281 caaaatggca tccacttctt tcagtgccga ttcgggcaac tgtttctcgg ccagttcttc
    20341 cacttttttc cagcgttcgt caaaggaaac agtcggttgc tgaaaggcag caaaagccac
    20401 cacaacaaaa actacggcaa gcaatataaa agaaagcttt ttcatgagga agaaatttta
    20461 aacggatatt tttatttgtg aagcgaaatg gaagacaaat atagtaccaa aatcggcagg
    20521 aagaaatttt ttgagcaaaa ttcgacaggg aaaattgttt ttgtagagat gttgcatgga
    20581 acggctctac tcagttctac caaaaggttg tccctgtgtg acttgaaagc tctgtaggag
    20641 cgaccttatg gttgagaaat ggaaaattat cactgccgta cgaaagtttt taaaaatgtt
    20701 gtttttgaaa attcatatat t
//
LOCUS       DAOTAX010000036        19440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2307_length_19440_cov_31.621047, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000036 DAOTAX010000000
VERSION     DAOTAX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19440
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_19440_cov_31.621047"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..3078)
                     /locus_tag="PLM04_05250"
     CDS             complement(304..3078)
                     /locus_tag="PLM04_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HON02515.1"
                     /translation="MRKIFFFLSLFFFPILIQAQTFSGSALITGKLIDKETKKPVPLA
                     NIRVLQQRDSSFVNGQASRNDGSFSIPVHYGSYIVHISFLGYDDVFKHVQVSASNPIA
                     VLDTIFLRQTDFKLSEVIITEKAPEVRVKGDTLEFNAGSFKVTESAVVEDLLKKMPGV
                     EIDKDGKITVNGREIKKIMVDGKEFFSSDPKVASKNLPAKMVEKVQVLDKKSEMAEMT
                     GFDDSDEETVINLVIKQGMKEGLIGNAFAGYGSKDRYEGNAMVNYMRNQNQFTFIGGL
                     NNTNNAGFTDLASSMFGNMGGRGMPGMFGNQDGVTTSGMGGFNFNTQLSQTFKLGGNV
                     RYGSTNSDVLSKIRTQNMLSTGNTFENENDTTNSFSQNFNMDLRMEWTPDTVTRIIFR
                     PSVGVYENSRDEISNFNTTNEENDSINKGDSKYSSDGNGYNVGGRLEVSRQLGKKGRV
                     LSLQLRGQLNDADNDGYNLSNTYYFGTKPDDIIDQKFTNTSNSKNWQSFLSYVEPIGH
                     NNFIQLAYNYQQKFSESNKDTRTKDDLGNYSVMDTTYSKRLENDFVNQSIEMNFKANR
                     GKYDYMFGFSMQPFNSSSKTYKGDSLYNNTSNQGINYAPSAQFNYRWSRQNNLRIRYS
                     GITSQPSVTQLSPVRDVSNPLNVTYGNPDLKPSFSHQLNIRYMNFMPQQKSSINFFGN
                     ASYTLNDIVSSTSTDVFTGQKETTYKNVNGNWNANGRLTLNLPIKKTNFSVFSMSSGS
                     YTHSKGYSNNEINLSQRTGLGQTLGIDFRSGVVDFGTRGNINYNNIQNSLTGQQDQEY
                     FNYNVSANTTIYLPYDLSIDSDLSYNTNSGYSTGFEQEALIWNASLQKQLFKHKNGSL
                     RLKIYDILQQKSNISRRATANYITDTTTNTLTSYFIVHFVYRFNIFKNGANQNDMMPH
                     RSFGGGPSGH"
     gene            3517..5391
                     /locus_tag="PLM04_05255"
     CDS             3517..5391
                     /locus_tag="PLM04_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02516.1"
                     /translation="MKIKQTKNISPWAWVPTLYFAEGIPYVVAMTVAVIMYKRLGISN
                     ADIALYTSWLYLPWVIKPFWSPFVDLIKTKRWWITSMQLLIGAGFAGVAFLIPAPFFF
                     QATLAVLWLIAFSSATHDIAADGFYMLALDSSQQSFFVGIRSTFYRFAMIAGQGILII
                     IAGALESHTGMEPVVFSVQSSPDPISQIVNVDSLSKVNFPEDAEMKFVVFPASLSLST
                     QPITSDSLNKIKAAALENNKRYQFIAEENLQNNQTKKENDSWWHQHVSGPFADFIREN
                     FGEKREKISMVENLTGNAGVVAIRLTKKPEAGKPLILNLSQSKGDKSISLVSGERLVF
                     TEENWDKPALVVVQLDAKLKGQAVSEFKGVSGNIRLAWSITFFILSGLFILFYLYHRF
                     ILPRPASDHPSLTHSSKDIIREFGRTFATFFQKKGIGLAIAFMLLYRLGEAMLVKMSS
                     PFLLDPHEVGGLGLTTGQVGLVYGTVGVIALTLGGIVGGIAASRKGLKYWIWPMALCI
                     TLPNLAYLYLSWFMPENMLLINAAVAFEQFGYGFGFTAYMLYMIYFSEGEHKTAHYAI
                     CTGFMALGMMLPGMMAGWLQELIGYNHFFIFVLICAIPTLAIIPFMKIDKEFGKKKSV
                     "
     gene            5571..6344
                     /locus_tag="PLM04_05260"
     CDS             5571..6344
                     /locus_tag="PLM04_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HON02517.1"
                     /translation="MKILILGAGKMGTFFTDLLCLHHEVALYDVDPQKLRFVFNTYRL
                     TQYKEIQEFEPELVINCVTLKYTIEAFKNVLPYLPENCMLSDIASVKTGLKEFYESTG
                     KRFVSTHPMFGPTFATLDNLSSQNAIIISESDYLGKMFFKDLYHSLHLKIFEYSFEEH
                     DKTTAYSLAIPFASTLVFAAVMKHQDAPGTTFKRHMDIAEGLLSEDDYLLTEILFNPY
                     AEEQIDNIRAQLKVLLDIIKKKDAARMQEFLEKVRKNIE"
     gene            6403..6579
                     /locus_tag="PLM04_05265"
     CDS             6403..6579
                     /locus_tag="PLM04_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HON02518.1"
                     /translation="MNTKEIVLEAMKKAGSPVNAGKIVEMTGLDRKEVDKAMNELKAE
                     GVIESPKRCYWQPK"
     gene            6883..8004
                     /locus_tag="PLM04_05270"
     CDS             6883..8004
                     /locus_tag="PLM04_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HON02519.1"
                     /translation="MPSIVIDSYIPYLRGVLDEVMDVTYLPHSQITNETVKNADALIV
                     RTRTKCDARLLSGSKVKFIASATIGYDHIDAAYCAENGIKWTNAPGCNALSVAQYMAS
                     VLSYVNRNYFELAGKTLGVVGVGAVGSKIVQLGASFGMNVLQNDPPRARKEDPYQFVS
                     LSEICSTSDIISFHTPLNMEGEDKTFHLADKKFFDTLKKKPMIINAARGEVISTSALL
                     DALNRQGILTAVIDCWENEPDIDTELLNKTALGTPHIAGYSAEGKANAASQSVRSVSK
                     FFGLGLDNWEVSNLPEPTKVDLSFVNNIEDFFLKTYNIESESNQLKNHPRLFEYLRSH
                     YPFRREPDAYLPQLNEIMQKQLIEQFPLFFNQKNFKTLA"
     gene            complement(8209..9123)
                     /locus_tag="PLM04_05275"
     CDS             complement(8209..9123)
                     /locus_tag="PLM04_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HON02520.1"
                     /translation="MKNIEAKNSILCRYLIANEQDTRWGLTITTVGKQSLSINEDYPP
                     KDGHPMGYLFSTEQGRVLDEYQLLYIVKGSGIFTSTSCKLTNINAGNMFMLFPGEWHN
                     YKSNPATGWEEYWIGFKGENIDKRVGNGFFCKENPVYKVGVLDEAVQLYNQGILVAIE
                     QKSGFQQMLAGIVNHLLGLAFCLNKYNEFENLEITNLINEAKIRFMEDFRKDIDIKDV
                     AKGLNMSYSTFRKVFKEYTGFAPAQFLQEIRIQHAKELLTNTNLNIKEIAFESGFENP
                     EYFFTVFKNKTGYTPIQYRNFTQEKISI"
     gene            9437..10915
                     /locus_tag="PLM04_05280"
     CDS             9437..10915
                     /locus_tag="PLM04_05280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HON02521.1"
                     /translation="MSKLPHFFAVDLGATSGRTILGTLQNNRLDLKELTRFQNKIICI
                     HDKYYWNIYSLFEEIKKGLLIASQQGIHINAMGIDTWGVDFVYLANDGSLLNLPRSYR
                     DPYTNGVPEEYFSRISKREVYELTGIQIMNLNSLFQLYAAKKESSSALEYADSILFMP
                     DALNYLFTKKKVCEYTIASTSQLLNPFTKKIEAELLVPMNIEPTILPQMVMPGTVVGN
                     LEKSIQRECNVPEIPFIAVAEHDTASAVVAVPAEDENFAYLSSGTWSLMGIETKNPII
                     TEESYRLNFTNEGGVDGTIRFLKNITGMWLLERCREEWKLDGNSYTYPEIVQMAESVR
                     SFQFLIDPDDSIFSNPESMTRSIAVFCRNSGQKVPATHAEFIRCIFDSLALKYKYVLE
                     SLQKLSPFPIKRLHVIGGGSQNKLLNQLTANAIGLPVIAGPAEATAMGNIMMQAKGLG
                     LINSLTEIRSIIRNSVELETFYPQNTELWEKAYKKYLHILEK"
     gene            10949..12208
                     /locus_tag="PLM04_05285"
     CDS             10949..12208
                     /locus_tag="PLM04_05285"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HON02522.1"
                     /translation="MKKDEIIQKSYEIAKERYEAVEVSVDKAMEVLEKLSLSIHCWQA
                     DDVTGFENFGNINDAGIQATGNYPGKARNMDELRQDIEKVLALVGGKHRLNLHAIYGD
                     FQGKKIDRNEIEPSHFTSWMQWAQEKGLKLDFNSTSFGHPKSGNLTLANPDKEIRNFW
                     IEHTKRCRLIAEEMGKFQNDPCIMNLWIHDGMKDLTVNRLLYRSILEKSLDEIFAKGY
                     TNMKDSIEAKLFGIGLESYTVGSYDFYLAYGTKNQKIVTLDTGHFHLTESVADKISSL
                     LLFVPEIMLHVSRPVRWDSDHVTILNDDVTELAKEIVRADALSRVHVGLDYFDASINR
                     IGAYVVGIRATQKAFFQALLEPVSLLRKYEDNGQYFERLALLEETKNLPWNAVWDYFC
                     LKNNVPVGEDYIAEIQKYEKNVTSKRN"
     gene            12345..13379
                     /gene="rhaT"
                     /locus_tag="PLM04_05290"
     CDS             12345..13379
                     /gene="rhaT"
                     /locus_tag="PLM04_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HON02523.1"
                     /translation="MNTILGLIIIAIGSIGQSSFYVPIKKVKNWSWESFWLMQGIFAW
                     LVFPFIGSLMAIPAGHSLGEIWSAGGSDMWKSVGYGILWGIGGLTFGLSMRFLGIALG
                     QSLALGTCSAFGTLIPALLKGDNLLKGDGLILLIGVCLAIAGIAVIGYAGSLKAKNLT
                     EEERKKAVKDFALKKGILIALLAGIMSACFNLGLESGSSIKTALLNIGGNSLMVLNPV
                     IFLVTIGGFLTNAVYCFYQNYKNQTFSDYTQSSGKIWMNNLLFCALAGVLWYSQFFGL
                     GAGQSFFAPGSVMMAFSWSILMSLNVLFSNIWGIILKEWNGVGKKTIFVLIIGLAILI
                     FSIIFPQIVK"
     gene            13848..15191
                     /locus_tag="PLM04_05295"
     CDS             13848..15191
                     /locus_tag="PLM04_05295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HON02524.1"
                     /translation="MAVVVIMPKQGQSVESCIVTEIKKKKGDTVKKGDILFSYETDKA
                     SFEEESPVDGVILECFHEEGDEVPVLQNVMIIGEPGENYSDLLAQTNISESVKEEPEI
                     KTRQSVAEVESSPAQPALAEPQSKNNAPISPRAKYLAEKEAIETSALTGSGPNGRIIE
                     KDVRAALENRPKLTPLAKKLASEEGLQPTTSGSGLNGIAKSVDLSAPTNAVYGLDYED
                     RKLPNIRKLIAKSMQASLQNSAQLTHHLGADARCILQLRKKAKAAYESGQLQVNITLN
                     DFVCFAVIKALQKFPNVNSHFLGDSIRYFKKIHLGLAVDTDRGLMVPVVRNADDLSII
                     GLASQLKEVAALCRKGNVNPEILSSEAGTFTVSNLGNYGVEIFTPIINLPQSAILGVN
                     TIVPRPKDLGDGVYGFLPYIGLSLTYDHRSIDGGEATRFLKQIAVEIENLEIELF"
     gene            15205..16563
                     /gene="lpdA"
                     /locus_tag="PLM04_05300"
     CDS             15205..16563
                     /gene="lpdA"
                     /locus_tag="PLM04_05300"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HON02525.1"
                     /translation="MTDLLIIGGGPAGYVAAERAGHHGLSVTLFEKKALGGVCLNEGC
                     IPTKTLLYSAKTYENALHADKYGVQTENVGYDYEKIISRKNKVVRKLVAGVKSKMAAN
                     KVTVIEGAATIVGRGQDGIEVSCNGNIYKGKNLLICTGSEATVPPIPGLKEAGDVVVT
                     NREILELKQQPKSLVVIGGGVIGMEFASFFNSLGTKVTIVEMLPEILGGLDFEISAML
                     RDIYAKKGIDFHLNAKVVQVDGHKVVFEKEGKTQTVEGEKILLSVGRRPVTQGFGLEN
                     LNVELNRGGIKTDEKMCTNVPGVFAAGDVTGFSLLAHTASREGEVVVNNLVGRQDRMR
                     YNAIPGVVYTNPEVAGVGETEESAKAKGIAYKVAKLPMSYAGRFVAENEGGNGLCKVL
                     VGEKYGEVIGVHLLGNPSSEIIYGACMAIEQEMTIKELQEVVFPHPTVSEIIKETIMS
                     EL"
     gene            16646..17074
                     /locus_tag="PLM04_05305"
     CDS             16646..17074
                     /locus_tag="PLM04_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HON02526.1"
                     /translation="MSSTIKTEYKYSELTSRIIGCALEVHKHLGNGFQEVIYQRALAY
                     EFELNGLQFHREFDMPVFYKEIQVGLRRVDFLVENVISVEIKAIIKLEDVHLAQAINY
                     LEAYNLEIGLLINFGERSLNFKRLTNKKYIPICHNNQLIK"
     gene            17093..>19440
                     /locus_tag="PLM04_05310"
     CDS             17093..>19440
                     /locus_tag="PLM04_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HON02527.1"
                     /translation="MPKSEFIDPNQVRQPGFIEFQAIPVNQYQKTVKDERSNFTDDEF
                     KSMYHDMVLIREFETMINLIKTKGEYNGTPYNHPGPAHLSIGQESAAVGMAWTLTVED
                     FIFGSHRSHGEILAKGMSAIHKLDDEQLMQIMENFFDGTILKVVQKDFNGTTKELARR
                     FLVYGTLAEIFARETGFNKGLGGSMHAFFTPFGVYPNNAIVGGSGDIAVGAALFKKVN
                     RKPGLVVANIGDAALACGPVWEGITFSAMDQFKQLWEGDMKGGLPIIFNIMNNQYGMG
                     GQTCGETMGYEIAARIGAGVNPEQMHAERVDGYNPLAVIDAYKRKRKVIEEKKGPVLL
                     DVLTYRYSGHSPSDASSYRTKEEVEAWEKHDCIVSFGKQMIEAGVATQDDLDNVWKDV
                     KALVYELFLKTIDDNISPRLKNPEVIGDMMFSNGSVDSFSDAKPEVLMPLEENSRVKK
                     IAAKERFAFDAEGKPYSKMKQFQLRDAIFEAIMDRFYKDASLIAYGEENRDWGGAFGV
                     YGGMTEALPYHRLFNAPIAEAAIVGTAVGYAMCGGRVIPELMYCDFLGRAGDEIFNQL
                     PKWQAMSGNVLKMPVVVRVSVGSKYGAQHSQDWTSLVSHIPGLKVCFPATPYDAKGLM
                     NAALQGTDPVVFFESQRIYDIGEQFHKGGVPTGYYEIPLGEPDIKKEGKDITFLTIGY
                     TLYQALEAAKELEEKYGMSVEVIDARSLVPFNYEKVIESVKKTGKIIIASDACARGSF
                     LNDMATNISQLAFDYLDAPVCVLGSRNWITPAHELETAFFPQA"
BASE COUNT         5873 a         3387 c         4014 g         6166 t
ORIGIN      
        1 agtatataaa aaattttaat tttttcagat ttttatattg aaaaataata gtataaatca
       61 ttgtttatgt gtttgttaca aaaaaataat ttttattcat gcaatacatc atgttcaagg
      121 gttatatttt gttcggaatt gctcttgtaa atcttttgga aaaagcaaag aaaaagaata
      181 taaaaacctt aatctgttat aacaactgaa tttttattac aaaatttgtc ctataaacag
      241 ttgcatttat gattttaata cagtagttat tgttctgttt tttcaagtat tgtcagcata
      301 gggtcagtgt ccactgggtc ctccaccaaa gcttctgtgc ggcatcatgt cgttttgatt
      361 tgcgccgttt ttgaaaatat tgaaacgata cacaaaatga acaataaaat aggaagttag
      421 cgtgttggta gtagtgtctg taatataatt ggccgttgct cttctgctga tgttgctttt
      481 ctgttgcaga atgtcgtaaa tttttaagcg cagggagcca tttttgtgtt taaaaagctg
      541 cttttgaagg gaagcattcc atatcagtgc ttcctgctcg aatcccgttg aatagccgga
      601 attggtattg taagataggt cgctgtcgat cgataagtca taaggtaaat aaatagtggt
      661 attggctgaa acattgtaat tgaaatattc ctgatcctgc tgacctgtca aactgttttg
      721 aatattgttg taattgatgt ttccccttgt gccgaaatcc acgacacccg aacgaaaatc
      781 aattcccagt gtttgtccca aaccggttcg ctgacttaag ttaatttcat tattggaata
      841 tcctttggaa tgagtataac tgcctgatga catggaaaaa acggaaaaat tcgttttttt
      901 tattggcaaa ttcagtgtca gtcgtccgtt ggcattccag ttgccattaa catttttata
      961 agtggtttct ttttgtcccg tgaaaacgtc tgtagaagtc gaagaaacaa tatcgttcaa
     1021 cgtgtaggaa gcgtttccaa aaaaattgat tgaacttttt tgttgcggca taaaattcat
     1081 atagcgaata ttcaattgat gggagaaaga aggttttaaa tcgggattac cataagtaac
     1141 gtttaaaggg ttggaaacat ctctcaccgg tgaaagttgc gttacggaag gttggcttgt
     1201 aatgcccgaa tacctgattc ttaaattgtt ttgtcgactc catcgataat tgaattgggc
     1261 acttggtgca taattgatac cctgattgga agtattgtta taaagagaat ctcctttata
     1321 ggttttactg ctggaattga acggctgcat actgaagcca aacatatagt catattttcc
     1381 tctgtttgct ttgaaattca tttctatcga ttggttaacg aagtcgtttt ccaatctttt
     1441 gctgtaggta gtatccatta ccgaataatt tcccaagtcg tctttggttc gtgtatcctt
     1501 gttcgattcg gaaaattttt gctgataatt gtaggcaagc tgaataaagt tattgtgtcc
     1561 gatgggttct acgtaggata gaaaactttg ccagtttttg ctgttgctgg tattggtaaa
     1621 tttctgatca ataatatcat caggtttggt accaaaataa taagtattgg atagattgta
     1681 accgtcgttg tcggcatcat tcaactgtcc tctcagttgg aggctgagaa cacgtccttt
     1741 tttccccaat tgcctgctta cttccaagcg cccgccgaca ttgtatccgt ttccatccga
     1801 agaatacttt gaatcacctt tatttataga atcattttct tcattggttg tattgaaatt
     1861 actaatttcg tcgcgactgt tttcatacac cccgacgctt gggcggaata tgatgcgggt
     1921 aactgtgtca ggagtccatt ccattcgtaa atccatatta aaattttgac tgaaactgtt
     1981 cgtggtgtca ttttcgtttt caaaagtatt tccggtactc agcatgtttt gtgtccggat
     2041 ttttgacaac acatcactgt ttgtggaacc ataacgaaca tttccaccca atttgaaagt
     2101 ttgagaaagt tgggtgttga aattgaatcc tcccatgccc gaagtagtaa ctccatcttg
     2161 gtttccaaac attcctggca taccgcgacc gcccatgtta ccaaacatac ttgatgccaa
     2221 atcggtaaat cctgcattat tggtattgtt taggccgcct atgaaggtaa actgattttg
     2281 gtttctcatg taattgacca tagcattgcc ttcgtagcgg tctttacttc catatccggc
     2341 aaaggcattt cctatcaatc cttctttcat cccttgctta ataaccagat ttatcacagt
     2401 ttcctcatcg ctgtcgtcaa agccggtcat ttcagccatt tcggattttt tatccagcac
     2461 ttgaactttt tctaccattt tggcaggtag atttttggac gcaactttgg gatcgctgct
     2521 gaaaaactct tttccgtcca ccataatttt tttaatttcg cgtccgttga cagtaatttt
     2581 cccgtcttta tcaatttcca caccgggcat ttttttaagt aagtcttcta ccacagcact
     2641 ttcagtaact ttaaacgaac cggcattaaa ttccagtgtg tctcctttca ctctaacttc
     2701 aggcgctttc tcggtaatta taacttcaga aagcttaaaa tcggtctgtc gcaaaaatat
     2761 agtatcaagc acagcaatag gatttgaagc tgatacttga acgtgcttaa aaacatcatc
     2821 ataacctaaa aatgagatat gtacaatgta agatccataa tgaaccggaa tggaaaaaga
     2881 tccatcattc ctgctcgcct gtccgtttac aaagctgctg tcgcgctgct gtaaaacccg
     2941 gatatttgct aatggtacag gtttttttgt ttctttatca atcagtttac ctgtaattaa
     3001 agcacttcca gaaaaagttt gcgcttgaat caggatgggg aaaaaaaata agctaagaaa
     3061 aaaaaatatt tttctcatta ctctgtctgt ttacgatatt ttttgtttca aaattatacc
     3121 tatttaaatt aatttccaaa aaagagaaaa tatttccctt gaaaaattga agggaaattc
     3181 agcatataaa tgtaagaatt gttggtagat gatttatcat tttttctttt aaattcacag
     3241 aagattgttt acctttgcct ttcttaattt gaaagcaatt gtttttcagt tcgatttttt
     3301 ctgtcgtggt ttgtattttt ctaattctta agcaaagaag ggatgttccc atatattgtt
     3361 ttacaatttt attgagttag aaatatttta aactcgtttc caaaatttat ttataaccct
     3421 caccctgact ttaattaaag ttgctatgaa aagtttatat gtttgaaatg atcagaaaca
     3481 gaatttataa cctctaaatt tttccgatat atcgacatga aaatcaagca aacaaaaaac
     3541 atttctccct gggcatgggt acccacttta tactttgctg aaggcattcc ttatgtagtt
     3601 gccatgacgg tagctgtgat catgtacaag cggctgggta tttcaaatgc cgatattgcc
     3661 ttgtatacca gctggttgta tttgccttgg gtaataaagc cattttggag tccatttgta
     3721 gatttaatta aaaccaagcg ttggtggatt acttccatgc agttgctgat tggtgccggt
     3781 tttgctggag tagctttttt aattcccgct cctttctttt ttcaggctac tttggctgta
     3841 ctgtggttga ttgctttcag ttctgcaact cacgacattg cggccgatgg attctatatg
     3901 ctggcgctcg atagctcaca acagtctttt tttgtgggaa ttcgcagtac cttttatcgt
     3961 tttgccatga ttgcaggtca gggaattctc attattattg cgggagcttt ggaaagccat
     4021 accggcatgg aaccggttgt tttttccgtt caatcttctc cggatccaat ttctcaaata
     4081 gtaaatgtag acagtctgtc aaaagtaaat tttccagaag atgccgagat gaaatttgta
     4141 gttttccctg ccagcttgag tttgagtacg caacctatta ccagcgattc gctcaataaa
     4201 atcaaagccg ctgcactgga gaacaacaaa cgctaccaat ttattgcgga agaaaacctt
     4261 caaaataatc agaccaaaaa agaaaacgat tcttggtggc atcaacatgt ttcagggcct
     4321 tttgccgatt ttatcaggga aaattttgga gaaaaacggg aaaagatttc aatggtagag
     4381 aatttaacgg gaaatgccgg tgttgtagcc atacgtttga cgaaaaaacc tgaagccgga
     4441 aaaccattga ttttgaattt aagtcagtcc aaaggcgata aaagtatttc tcttgtcagt
     4501 ggcgaacgtt tggtttttac cgaagaaaat tgggataaac ccgcgctggt agttgttcag
     4561 ctcgatgcta agctgaaggg acaggcggta agcgaattta agggagtttc aggaaatatc
     4621 agattagctt ggagcataac tttcttcatt ttgtccggtt tatttatctt attttatctg
     4681 tatcacaggt ttattttacc ccgaccggct tccgatcacc catctttgac tcattcgtca
     4741 aaagatatta ttagagaatt tggacgaact tttgccacat ttttccaaaa gaaaggaatc
     4801 gggttggcta ttgcattcat gcttctttat cgtttgggtg aagcaatgct ggtgaaaatg
     4861 tcttctcctt ttttactcga tccgcatgaa gttggaggtt tgggactaac tacaggacaa
     4921 gttgggctgg tttatggaac ggttggcgtc attgccttga ctttgggagg aattgtgggc
     4981 gggattgcag cttcgcgcaa aggattgaaa tattggattt ggccgatggc cttgtgcatc
     5041 acgctgccaa acttggctta tttgtatttg tcgtggttca tgcccgagaa tatgttgctt
     5101 attaatgcgg cagtcgcttt cgaacagttt ggttatggat tcggattcac ggcttatatg
     5161 ctgtatatga tttatttttc ggaaggagaa cacaaaactg cccattatgc catttgtacg
     5221 ggattcatgg cgctgggaat gatgttgccc gggatgatgg ccggttggct gcaagaattg
     5281 atagggtaca atcatttctt tattttcgtg ctgatttgtg ccattccgac tcttgccatt
     5341 attccgttta tgaagataga caaggaattc gggaaaaaga aatctgtgta agaaatcttt
     5401 ctacttattt gtaaaatgaa tcgttttgtt ttcatttttg ccacttttgt attcgaaatt
     5461 gaaaaataac aatttatgaa acccacatgt atcccataca caaacacgaa tgaatataat
     5521 attcagacat gtaggttcca tcagaccttt aaaaaataaa ttatattctg atgaaaatat
     5581 tgattttagg agcagggaag atgggcactt ttttcaccga tttattgtgc ctccatcacg
     5641 aagtggctct ttatgacgtt gacccacaaa agttgcgttt tgtttttaat acttatcggc
     5701 tgactcaata caaagaaata caagagtttg aacctgaatt ggtaatcaat tgtgttacgc
     5761 ttaagtatac catagaggca tttaaaaatg tgttgcccta tttgcccgaa aactgcatgt
     5821 tgtccgatat tgcatcggtt aaaacgggtt tgaaggaatt ttacgaaagt accggaaaac
     5881 gttttgtttc cactcacccg atgtttggac caacatttgc aacgctcgac aatttaagtt
     5941 ctcagaatgc catcattatt tcggagtccg attatcttgg aaaaatgttt ttcaaagatc
     6001 tgtaccattc tctacattta aaaatctttg aatactcttt tgaagaacac gataaaacaa
     6061 cagcatattc gctggctata ccatttgcat ccacattggt ttttgcagct gtcatgaaac
     6121 atcaggatgc acctggcacc actttcaaaa ggcacatgga cattgcagaa gggcttttat
     6181 cggaagacga ttatctgttg actgaaattc ttttcaatcc ctatgcggaa gaacaaatcg
     6241 acaacattcg agctcaactg aaagtgttgc tggacatcat taaaaagaag gacgcggcac
     6301 gaatgcagga atttttggaa aaagtcagaa aaaatatcga ataagctttg tattgaaaac
     6361 attttcggga aatgattgtt gtaaacaaaa aaattaacag ctatgaatac taaagaaatt
     6421 gtactggaag ccatgaagaa ggccggttcg ccggtgaatg caggaaaaat tgtcgaaatg
     6481 accggtctcg acagaaagga agtagacaaa gccatgaacg agctgaaagc cgaaggagtc
     6541 atagaatcac ccaaaagatg ttattggcaa ccaaagtaat tagaaaagtt taggcaaaaa
     6601 agggttcaag ggttatttca aaaaattttt cttgaactga ttactaataa taaagcgtct
     6661 gagaatttat tgatttttaa ggcgctttat cgtttaatca atgtttataa agtctgtttt
     6721 tacgcccagg atagtaaaat tattgtcgaa aaataacttt ttaaaccatt ttatttgatt
     6781 tctcacttgc ctgttggctt tacaactttg atttttattt tcacaagctt ggaaataatt
     6841 caattttcat gtaattttgc agttcaatta tttcaatcaa aagtgccaag tattgtcatt
     6901 gacagttata ttccttacct tcggggtgta ttggatgagg tgatggatgt tacatatctg
     6961 ccccattctc agattacgaa tgaaaccgta aaaaatgcag atgcccttat agtccgtacg
     7021 cgtaccaaat gcgatgcacg tttgttgtcg ggttctaaag ttaagtttat tgcctcagcc
     7081 actattggtt acgatcatat agatgctgca tattgtgccg aaaacggtat aaagtggacc
     7141 aatgcgcccg gttgcaatgc gctttcggtt gctcagtata tggcttcggt gctcagttat
     7201 gtaaatagaa attattttga gctggcagga aaaactttag gagtggtcgg ggtaggagct
     7261 gtaggttcaa aaattgttca attgggagct tcttttggta tgaatgtgtt gcaaaacgat
     7321 cctcctcgcg ccagaaaaga agacccttac caattcgttt ctttatcgga aatttgttcg
     7381 acatccgata ttatctcgtt tcacacaccc ctcaatatgg aaggtgaaga taaaactttt
     7441 catttagccg ataaaaaatt tttcgataca ttaaaaaaaa aaccgatgat tattaatgct
     7501 gctcgaggag aagtaatttc tacaagtgca ttgttggatg ctttgaacag acaaggaata
     7561 ttgactgctg ttattgattg ttgggaaaat gagcctgata ttgataccga attgttgaat
     7621 aaaactgcat tgggtactcc acacattgcc ggatattcgg ccgaaggcaa agccaacgct
     7681 gcttctcaaa gtgtgcggtc ggtaagcaaa ttttttggtc tgggacttga taattgggaa
     7741 gtctctaatc tccctgaacc tactaaagtc gatttgtcgt ttgttaataa catagaagat
     7801 ttttttctga aaacttacaa tatcgaatca gaaagcaatc agttaaaaaa tcatcctcga
     7861 ctgtttgagt atctgagatc tcactaccct tttcgacgtg agccggatgc ttacctgcca
     7921 caattgaatg aaattatgca gaaacagtta attgaacaat ttcctctttt ttttaatcaa
     7981 aagaatttca aaacgcttgc ctaaggatta tttattctaa taagataaat taatgggcaa
     8041 ctgctaataa aatcagagat ggtatgaaat acgtttgctc ttacctgtaa agaaaaagtt
     8101 taatggaaat tacattcata atatgttgtt aatctgtcat tcaaatcatt aaaaagaagc
     8161 acataatttt cggaataaac aaaatagcaa aaaacagaat gacaattttt agatgctaat
     8221 tttctcttga gtaaaatttc gatattggat gggagtatat ccggttttgt ttttaaaaac
     8281 agtaaaaaaa tattccggat tctcaaatcc gctctcaaat gctatttcct taatattcaa
     8341 gtttgtgttt gtcaataatt cttttgcatg ttgtatacgt atctcttgaa gaaattgtgc
     8401 cggagcaaaa ccggtatatt ctttaaaaac cttacgaaaa gttgaataac tcatatttaa
     8461 tcctttcgca acatctttaa tatctatatc tttccggaag tcttccataa atctgatttt
     8521 agcttcgttt ataagatttg taatttccag attttcaaat tcattatatt tatttaaaca
     8581 aaaagctaat cctaacagat gatttactat tcctgccaac atttgttgaa aaccactttt
     8641 ctgttctatt gccactaaaa ttccttgatt atacaactga acagcttcgt ccaatactcc
     8701 taccttataa acgggatttt ctttacaaaa aaagccattt cctacacgtt tatctatatt
     8761 ttctccttta aatccaatcc agtattcttc ccatcctgtt gctggatttg atttataatt
     8821 gtgccattcg ccagggaaaa gcatgaacat atttcctgca tttatattgg taagcttgca
     8881 agaagttgat gtgaaaattc ctgaaccttt aacaatatat aataattgat attcatccaa
     8941 tacacgacct tgttcggtgg aaaataaata tcccatagga tgtccatctt ttggcggata
     9001 atcttcattg atgcttaagc tttgtttacc aaccgtagtt attgtcaatc cccatcttgt
     9061 atcttgctcg tttgcgatga gatagcggca aagaattgag ttttttgctt ctatgttttt
     9121 catgtattaa tctgcttgta aaattagaga caaaggaaac aagtaaatat ctgaaaatta
     9181 aacgtctatt tcttttctcc gatccttaaa gatttgaaat ttcatgacaa aattaatttt
     9241 ttttttgaac ttgtgaaggg aaaaattttt attccacaga ttacaagaaa aaaaatttac
     9301 attttatgcc aaatttcaaa gtatttatat caaaatactt agtattttct tatggttatt
     9361 aatgtacttt tgttgaaatt tacattaggt agattcatta ataaattttc tgcattcata
     9421 atacaagata aattctatga gtaaactccc tcattttttt gctgtagatc tgggtgccac
     9481 cagtgggcga accattttag gtactttgca aaataaccga cttgatttaa aggaacttac
     9541 tcgttttcag aataaaatta tttgtattca tgacaaatat tattggaaca tttattcatt
     9601 atttgaagaa attaaaaaag gacttttaat tgcaagtcag caaggaatcc atatcaatgc
     9661 aatgggtatc gatacatggg gagttgattt tgtgtatctg gcaaacgatg gttctttact
     9721 taatcttccg cgttcctatc gtgatccata tactaacgga gtaccggaag aatatttttc
     9781 acgtatttca aagagagaag tttatgaact gacaggcatt cagattatga atttaaacag
     9841 cttgtttcaa ttgtatgcag caaaaaaaga atcttcttca gctcttgaat atgctgattc
     9901 aattttgttt atgcccgatg ctcttaatta tttgtttacc aagaaaaaag tttgcgaata
     9961 taccattgct tcaacttctc aattgttaaa tccattcaca aaaaaaattg aagccgaatt
    10021 attagttcca atgaatattg aaccaacaat actaccacaa atggttatgc cgggtacagt
    10081 tgttggaaat ttggaaaaaa gtattcaacg ggaatgcaat gttcctgaaa ttcccttcat
    10141 cgcagttgca gagcatgata ctgcttcggc agtggttgca gtgccggcag aggacgaaaa
    10201 ttttgcctac ctgagttcgg ggacatggtc attaatgggc attgaaacca aaaatcccat
    10261 tattacagaa gaaagttatc gtttaaattt taccaatgaa ggtggagtag atggtaccat
    10321 acgattcttg aaaaacataa caggcatgtg gcttttggaa agatgccgcg aagaatggaa
    10381 attggatggg aattcctata cgtatcctga aattgttcaa atggcagaat cagtaaggag
    10441 ttttcaattt ttaattgatc ctgatgacag tattttttca aatcctgaaa gcatgacacg
    10501 gtcaatagct gttttttgtc ggaatagtgg tcaaaaagtt cctgctacac atgctgaatt
    10561 cattcgttgt atctttgaca gtttggcatt gaaatataag tatgttctcg agtcattgca
    10621 aaaattgtct cctttcccta taaaacgact tcatgttatt ggaggtggct ctcaaaataa
    10681 attgcttaat cagctcacag cgaatgctat aggacttcct gtgattgcag gacctgcaga
    10741 agctactgcg atgggaaaca ttatgatgca ggcaaaagga ttggggctga tcaattcatt
    10801 aactgaaatt aggtctatca tccgaaattc tgttgagctg gaaacttttt atcctcaaaa
    10861 tacagagtta tgggaaaaag catacaagaa atatcttcat attttagaaa aataaaagtc
    10921 gaaataacta ttacaaaatt aatgtatcat gaaaaaggat gaaattattc aaaagtccta
    10981 cgaaattgca aaagaacgtt atgaagcagt agaagtaagt gttgacaaag caatggaagt
    11041 tctcgaaaaa ttatctcttt ccattcactg ctggcaagcc gatgatgtga cgggttttga
    11101 aaattttggc aatatcaacg atgcaggtat tcaagccact ggcaattatc cgggaaaagc
    11161 acgcaatatg gatgaacttc gtcaagatat agaaaaagtg ttggcattgg taggaggtaa
    11221 acatcgctta aatctccatg ctatttacgg agattttcaa gggaaaaaaa ttgaccgcaa
    11281 cgaaatagag ccttcccatt tcaccagctg gatgcaatgg gctcaagaga aagggctaaa
    11341 acttgatttt aattcgacat cttttggaca tccaaaaagt ggtaacctca cgttggcaaa
    11401 tcccgacaaa gaaattcgca atttttggat tgaacacacc aaacgttgtc gactaattgc
    11461 cgaagaaatg ggaaaatttc aaaacgaccc ttgtattatg aatctttgga ttcacgacgg
    11521 gatgaaagat ttaactgtaa atcggttgct ttacagaagc attttggaaa aatcgttgga
    11581 tgaaattttt gccaaaggtt acacaaacat gaaagacagt atagaagcaa aactttttgg
    11641 tattgggttg gaaagttaca cggtaggttc atatgatttt tatctggctt atggtactaa
    11701 aaaccaaaaa atagttacgt tggatacagg ccattttcat ttgacggaaa gtgttgctga
    11761 taaaatatct tctcttttgc tttttgttcc ggaaattatg ttgcatgtaa gtcggccggt
    11821 gcggtgggat tccgaccatg taacgatatt aaatgatgat gttactgaat tggctaaaga
    11881 aattgtacgg gcagatgcct tgagtcgagt tcatgtgggg ttggattatt tcgatgcttc
    11941 tatcaatcgc atcggagcgt atgttgttgg catacgggct acgcagaaag ctttttttca
    12001 ggccttgcta gaacctgttt ctttattgag aaaatatgaa gataatggac aatattttga
    12061 acggctggca ttgctcgaag aaaccaaaaa tttgccctgg aatgctgttt gggactattt
    12121 ttgcttgaaa aacaatgtgc ctgtcggaga agattatatt gctgaaattc aaaaatatga
    12181 gaaaaatgta acctcaaaac gaaattaaat ttcatcagaa tataatttat tttttaaagg
    12241 tctgatggaa cccacatgtc tgaatattat attcattcgt gtttgtgtat gcaatacatg
    12301 tgggtttctt gattaaaaat tttttaaaag ccatttttta tatcatgaat accatcttag
    12361 gattaataat tattgccatc ggtagtattg gacagtcaag tttctatgtc cccataaaaa
    12421 aggtcaaaaa ttggtcgtgg gaaagtttct ggctgatgca aggtattttt gcatggcttg
    12481 ttttcccatt tataggctca ttaatggcaa ttcctgccgg acattcgctg ggagaaatat
    12541 ggtctgcggg tggtagtgat atgtggaaat ctgttggata tggtattctt tggggaattg
    12601 gaggcttgac ctttggtctt agtatgcgtt ttttgggaat tgcgttagga cagtcattag
    12661 ctttgggaac atgctccgca ttcggaacat tgataccggc tttattaaaa ggtgataatc
    12721 tgttgaaagg cgatggattg attctgttaa taggagtttg tcttgctatt gccggtattg
    12781 cagtgattgg ttatgccggt tcgctaaaag cgaaaaattt aaccgaagaa gaaagaaaaa
    12841 aggcagtaaa agattttgct ttgaaaaaag gaattctcat tgctttgctg gctggtatta
    12901 tgagtgcatg ttttaatttg ggtttggaat ccggaagctc tataaaaact gctcttctga
    12961 atattggagg gaacagttta atggtactga atccggtgat ttttctggtt actattggtg
    13021 gttttctaac aaatgcagtt tattgttttt atcaaaatta taaaaatcaa actttttccg
    13081 attacactca atcttcaggg aaaatctgga tgaataattt gttgttttgc gccttggcag
    13141 gcgttctctg gtattcgcag tttttcggat tgggtgccgg tcaaagtttt tttgctcccg
    13201 gaagtgtcat gatggcattt tcatggagta ttcttatgtc actgaatgtg ttgtttagca
    13261 atatttgggg aattatattg aaagaatgga atggcgttgg taaaaaaaca atttttgttc
    13321 ttattattgg gttggccata ttgatattct ctattatatt tccccaaatt gtaaaataaa
    13381 catgtaattt atcgcaaatg gaattgctca tcaataggta cttgacggtt gcaccaagca
    13441 attgagtcag aaacaagcat aagaataggt atccttctac ctccgaattt actacaaatg
    13501 ttttaataaa attaatttga cagatatgtt attaacttct cacacatttc gacggaaagt
    13561 gaattcttca aaattttttc ataattttgc atttgctggt tgaatctact attaatgttt
    13621 tgtaaaaaaa tattttgaag ttttgataac aatccttatt cttaggctat tttgaaattt
    13681 tatttagaaa acatcaattt cataatagat ttattttctg gtaacatgcg tttctaaata
    13741 aaaaattatt tttcttttat ctaattttac tttacttatt aagtcaaatt ctttcaaaag
    13801 gaagaatatc ccatttgaaa gaggcatttt atttaaatta tttatctatg gcagttgtgg
    13861 taattatgcc caaacaaggg caatcggtgg aaagttgtat tgttactgaa ataaaaaaga
    13921 aaaaaggaga caccgtaaaa aaaggagata ttttgttttc gtatgaaacg gataaagcct
    13981 cttttgagga ggaatctccg gtagatggcg ttatattgga atgttttcat gaggagggag
    14041 acgaagtgcc cgtgctgcaa aacgtgatga ttatcggtga acccggagaa aattattccg
    14101 atttgcttgc acaaaccaat atttcggaat cggtaaagga agaacccgaa atcaaaacgc
    14161 gacaatctgt tgccgaagtc gaaagttctc ccgcacaacc tgctcttgca gaacctcaat
    14221 caaagaataa tgctccgatt tcaccccgag ccaaatatct tgccgagaaa gaagcaatcg
    14281 aaacgtctgc tcttactggt tcgggaccta atggtcgtat tatcgaaaaa gatgttcggg
    14341 ctgcactcga aaatcgtccc aaattgacac cgttggcaaa aaaacttgct tcggaagaag
    14401 gtttgcaacc aactacatcg ggaagcggat taaatggaat agcaaaatcg gtagatttat
    14461 cagctccgac aaatgcagtt tatggtttgg attacgaaga tcgaaaattg cctaatatcc
    14521 gcaagctaat tgcaaaatcg atgcaagctt cgcttcaaaa ttcggctcag ttgacgcatc
    14581 atctgggtgc cgatgcccga tgcattctcc aacttcgcaa aaaggcaaaa gctgcctacg
    14641 agagcggaca attgcaggtg aatatcacgt tgaacgattt tgtctgcttt gccgtgatca
    14701 aggctttgca aaaatttccg aatgtaaatt cacattttct gggcgacagc attcgatatt
    14761 tcaagaaaat tcatctcggt ttggcggtcg ataccgatcg gggattgatg gtgccggtgg
    14821 tgcgcaatgc cgacgacttg tccattatcg gattggcaag ccagcttaag gaagtggcag
    14881 ctttatgtcg aaaagggaat gtgaatcccg agattctttc atcagaggcg ggtacgttta
    14941 ccgtatccaa tttgggaaat tatggtgtag agatttttac gccaatcatc aatttgcctc
    15001 aatcggcaat tttgggagtg aatacaattg tgcctcgccc gaaagatctg ggagatggag
    15061 tttatggatt tctcccatac atcggattga gcctcaccta tgatcatcgt tcgattgacg
    15121 ggggcgaggc tacacgcttt ttaaaacaaa ttgcggtgga aatagaaaat ctcgaaatcg
    15181 aactttttta aattgaaaca ccttatgaca gatttattaa ttatcggcgg cggcccggca
    15241 ggatatgtgg cggctgaaag agcaggacat catggattgt ctgtgacgct tttcgaaaaa
    15301 aaggccttgg gcggagtgtg cctcaatgaa ggttgtattc ccacaaaaac tttgctctac
    15361 agtgcaaaaa cctacgaaaa cgcattgcat gcagataaat atggcgtaca gaccgaaaat
    15421 gttggatatg attacgaaaa gatcatcagt cggaaaaata aagtagtacg aaaattagtt
    15481 gcaggcgtca aaagtaaaat ggcagccaac aaagtaaccg ttattgaagg cgctgccaca
    15541 atagttggtc gtggacaaga cggaatcgaa gtatcctgca atggcaatat atataaaggt
    15601 aaaaacctgc tgatttgcac cggctccgag gcaaccgtgc ctcccatacc cggattaaag
    15661 gaagcaggcg atgtggttgt taccaatagg gaaatacttg aactgaaaca gcagccaaaa
    15721 tctctcgttg tgatcggggg aggcgttatc ggaatggagt ttgccagttt tttcaatagt
    15781 ttgggaacga aagttacgat tgtggaaatg ttacccgaaa tattgggcgg actcgatttc
    15841 gagatttcgg ccatgcttcg tgatatttat gccaaaaaag gaattgattt ccacttgaat
    15901 gcgaaagtgg tacaggtgga cggtcataaa gtagttttcg aaaaagaggg caaaacacag
    15961 acggtcgaag gagaaaaaat ccttcttagt gtaggtcgtc gtcctgtaac gcaaggattt
    16021 ggattggaaa atctgaatgt ggaactaaac agaggcggca tcaagaccga cgaaaaaatg
    16081 tgcactaacg tacccggcgt cttcgcagcc ggcgatgtta cgggtttctc tctgctggct
    16141 catacggcaa gtcgcgaagg agaagtggtg gttaataatc ttgtcggacg tcaggacagg
    16201 atgcgttata atgctattcc cggcgtggtt tacaccaatc ccgaagtagc aggtgtgggc
    16261 gaaacggaag aatcggctaa agccaaaggc atagcctaca aggttgccaa acttccgatg
    16321 tcttatgccg gacgttttgt cgccgaaaac gaaggtggca atggcctttg caaagtcttg
    16381 gtcggagaaa aatacggcga agttattggt gtgcatttgt tgggaaatcc gtcgagcgaa
    16441 ataatttatg gtgcttgcat ggctatcgaa caggaaatga ctataaaaga attgcaggaa
    16501 gttgtttttc cgcacccaac cgtgagcgag attatcaagg aaaccatcat gtcagaactt
    16561 tgattttatt gatcaaatga ttcttatgat taaatcgcac atcatctttc agtatagtat
    16621 agtcattttt taaattgatt acagcttgag ttcaacaatt aaaacagaat acaaatattc
    16681 cgaactcacc tctcgaatta ttggatgtgc tttggaagta cacaagcatt taggcaatgg
    16741 atttcaagag gtaatttatc agcgtgcatt agcatatgaa tttgaattga atggattaca
    16801 attccatcga gagtttgata tgcctgtttt ttacaaagaa atacaagtcg gtttacgacg
    16861 agtagatttc ttggtagaaa atgtaatttc agtcgaaata aaagctatca tcaaattgga
    16921 agatgttcat cttgctcaag caataaatta tttggaagcg tataacttag aaattggctt
    16981 actgattaat ttcggtgagc gaagtttaaa ttttaaaaga ttgacaaaca aaaaatatat
    17041 accaatttgt cataataatc aattaatcaa ataaataata gttcaaacta tcatgccaaa
    17101 aagcgaattt atcgatccca atcaagtacg acaaccggga tttatcgaat ttcaagctat
    17161 tcctgtcaac cagtatcaaa aaacggtaaa ggatgaacga agcaatttta cagatgacga
    17221 atttaagtcc atgtatcatg atatggtttt gattcgcgag ttcgaaacca tgatcaatct
    17281 tatcaaaacc aaaggtgagt ataacggaac gccctataat catcccgggc ctgcacatct
    17341 ttccatcggt caggaatctg cagcagtcgg catggcttgg acgcttactg tcgaagattt
    17401 catttttgga agtcaccgtt cgcatggtga aattcttgca aaagggatgt cggcaattca
    17461 taaactcgat gatgagcaat tgatgcaaat catggagaat ttcttcgacg gaactatttt
    17521 gaaagtcgtt caaaaagatt tcaacggaac tacaaaagaa ctcgctcgcc gatttttagt
    17581 atatggaacg cttgccgaaa tatttgcgcg cgaaacggga ttcaataaag gtttaggtgg
    17641 atcgatgcac gcatttttca cccccttcgg cgtttatccc aacaatgcca ttgttggtgg
    17701 ttcgggcgat attgccgttg gcgctgcgct tttcaagaaa gttaatcgca aacccggttt
    17761 ggtcgttgcc aatatcggcg atgccgcatt ggcttgtggc cctgtgtggg aaggaattac
    17821 tttctcggcg atggatcaat tcaaacagct ttgggaaggc gatatgaagg gcggattacc
    17881 catcattttc aatatcatga ataatcagta tggcatgggc ggacaaactt gtggcgaaac
    17941 gatgggctac gagattgcag cacggattgg agcaggcgtc aatcctgagc aaatgcatgc
    18001 cgagcgcgtt gacggataca atccgcttgc tgtcatcgat gcttataaac gtaaacgaaa
    18061 agttattgaa gagaaaaagg gacctgtctt gctcgatgtg cttacttatc gctatagcgg
    18121 gcattcgcct tcagatgctt cttcgtatcg gacaaaagaa gaagttgagg catgggaaaa
    18181 gcatgattgc attgtttcgt tcggtaaaca gatgattgaa gccggagtgg ctactcaaga
    18241 cgatttggat aatgtatgga aagacgtaaa agcgctcgtt tacgagttgt ttctgaagac
    18301 tattgacgac aatatttccc cgcgtttaaa aaatccggag gttatcggcg atatgatgtt
    18361 ttcgaatggc tcggtagatt ctttctctga cgccaagccc gaagtgttga tgccacttga
    18421 agagaattcg cgtgtgaaaa agattgctgc caaagagcgt tttgcattcg atgccgaagg
    18481 caaaccttac agtaagatga aacaatttca gctgcgcgac gccattttcg aagctatcat
    18541 ggatcggttt tataaagatg cctcacttat cgcctacggc gaagaaaatc gagattgggg
    18601 aggggctttt ggcgtatatg gcggaatgac ggaagcgttg ccttatcatc gtcttttcaa
    18661 tgcgcccatt gccgaagcag cgattgtggg aacggctgtc ggatatgcca tgtgcggagg
    18721 acgcgtcatt cccgaattga tgtattgcga ttttctggga cgtgcaggcg atgaaatttt
    18781 taatcaattg cccaaatggc aggcaatgag cggtaatgtc cttaaaatgc ccgttgttgt
    18841 tcgtgtatct gtcggttcta aatatggtgc acaacattcg caggattgga catcgttggt
    18901 ttctcatatt ccgggtctga aagtttgttt tccggcaact ccgtatgatg caaaagggct
    18961 gatgaatgcc gctttgcagg gaaccgatcc ggtagtattc ttcgaaagtc agcgcattta
    19021 cgatatcggt gaacagtttc acaaaggtgg agtgccgacc ggatattacg aaattccatt
    19081 gggtgaacca gacatcaaga aagaagggaa ggatatcacg tttcttacaa ttggttacac
    19141 cttatatcaa gcattggagg cagcaaaaga actggaagaa aaatacggca tgagtgtcga
    19201 agtcatagac gcacgatcgt tggttccttt taattatgaa aaagtaatcg aatcggtgaa
    19261 aaagacggga aaaataataa tcgcaagtga tgcatgtgcg agaggttcgt ttttaaacga
    19321 tatggcgacc aatatcagtc aacttgcttt cgattatttg gatgccccgg tttgtgtgct
    19381 gggatcgcgt aattggatta caccggctca tgaactcgaa acggcatttt tcccgcaggc
//
LOCUS       DAOTAX010000037        19222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2348_length_19222_cov_31.890176, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000037 DAOTAX010000000
VERSION     DAOTAX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19222
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_19222_cov_31.890176"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLM04_05315"
     CDS             <1..371
                     /locus_tag="PLM04_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HON02528.1"
                     /translation="NDSKATNVNSCWYALESMKTPVILILGGTDKGNDYSEIESLVKE
                     KVKALVFLGVDNTPLHRFFDGKIDKIVDVRSMEDAVRAAYAMAEEGDTVLLSPCCASF
                     DLFQNYEDRGRQFKTCVRNL"
     gene            410..1642
                     /locus_tag="PLM04_05320"
     CDS             410..1642
                     /locus_tag="PLM04_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HON02529.1"
                     /translation="MENFLKKYLKGNATIWIVFILLCLVSILEMYSASSTLAFKSSNT
                     AMPLLRHAGFLLFGVILAFLVHLIPYKYIRLLAYLGLFLSFILLILVYYKGQSANDAT
                     RWFEIGGLQFQPSELAKLSVIIVVADFMSRIKNEAADEKKYFRYILIVLAAICPLILF
                     ENFSTAMLLFGVVFLMMFIGGISRKKLGLLLLSITLFVLGGYAMIKTVPEDSMPKIFD
                     RAYTWSARIDRYTEEKKQEENKYVITDENRQVQHGRIAIARGGMLGVMPGNSVERDYL
                     PQAYSDFIYAIIVEEMGLVGGVIIILFYLVLLYRAGRIAVKSETVFPALLVIGLSLMI
                     VLQAFVSMAVATSLGPVTGQPLPLISRGGTSIIITCIYFGIILGVTRQLKGEQHVEEE
                     IPEEQERNFPVVDLNELS"
     gene            1725..2831
                     /gene="murG"
                     /locus_tag="PLM04_05325"
     CDS             1725..2831
                     /gene="murG"
                     /locus_tag="PLM04_05325"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HON02530.1"
                     /translation="MPYRFIISGGGTGGHIFPAISIANALKKQLPNAEILFVGALGRM
                     EMERVPAAGYPIKGLPVSGFDRKNPFRNVKVLLNLRRSLQMARQIIRDFKPDAVIGVG
                     GYASAPTLKAASDLGIPTLIQEQNSYAGITNKLLAKKAARICVAYEGMERFFPKEKLV
                     LTGNPVRQELLSIPEKSKEAFEFFGFRPEKPTLLVVGGSLGARTINRSIISHLQELSQ
                     ADIQIIWQTGKFYIEEARKAAEPFSSPQLIVTDFVSRMDLAYSVADLVISRAGAGSIS
                     ELCLLGKPAILVPSPNVAEDHQTKNALALVQKEAAVMIKDEEAEKMLIPKALELIKDA
                     DSLNKLGQNIRKLAQKDSAERIAAEVLQLISKKN"
     gene            2878..4260
                     /gene="murC"
                     /locus_tag="PLM04_05330"
     CDS             2878..4260
                     /gene="murC"
                     /locus_tag="PLM04_05330"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HON02531.1"
                     /translation="MDFTKYYFLGIGGMGMSALARYFNSKGFQTAGYDRVQSKVTADL
                     QTEGIQINFDPNVDQIPISFRYPENTLVVVTPAIPDDLPQLVYFRENGFTIKKRAEVL
                     GDITRKASTICIAGTHGKTTTSTLTAHLLYQSHISCNAFLGGIANNYNSNLLLSKDSN
                     LFVIEADEYDRSFHQLSPYMAVITSADPDHLDIYQTPEAYRESFEHFTSLISPGGVLL
                     MKKGVNIQPRLQKGVKQYTYSMNDGGDFYAENIHLVNHEFHFDFVTPSERISDIRLKI
                     PIRINIENSVAAIALAWLNGVTKEEIRTAIASYSGIYRRFNLVFRSEQTIFMDDYAHH
                     PKELKASILSIKELFPEKKITGIFQPHLYSRTRDFASDFAEALSLLDELILLDIYPAR
                     EEPIEGISSEMIFRNVKLDNKTLCSRENLLSLLKDKHPEVLVTFGAGDIDKLVPLIKK
                     QLMEQTNGNA"
     gene            4383..5132
                     /locus_tag="PLM04_05335"
     CDS             4383..5132
                     /locus_tag="PLM04_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HON02532.1"
                     /translation="MKPVFKYILITFGILLVTGYLAWALLTFPDPKANEVCNRFHIVF
                     LDQSGVKLISEKEVAEMIDIKGLNPMGKTLKHVRTEEIEELLTKHPFIKQAECFKTPS
                     GGVYLFLQQRHPKFRLAGNENFYVDDEKNIIPVSYNDAARLPVVSGLVTKSMAKGELF
                     DFVSYLEENPFWNAQIEQIYIRPDRKIELVPRVGTGIILLGNLKDYEKKLHKLKILYE
                     KGFNEFGWNYYKTIDLQYENQIVCTKTDNID"
     gene            5177..6439
                     /gene="ftsA"
                     /locus_tag="PLM04_05340"
     CDS             5177..6439
                     /gene="ftsA"
                     /locus_tag="PLM04_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HON02533.1"
                     /translation="MNQNTYIALDLGSSRITAMAAEVLESGAVKVLATESKAADDVYH
                     GIIEHTSGAAFKISELLKLLQNSAKIRDIQQVCVALNAKSMKLSKSSTTRFIGQGKIV
                     TDALLDEMKEECERKNSDENNIVFDVIPVSFEIDGKITTDPVGQSGNQITGYYHVVKG
                     NARILQQLKSCFVRNQIQIEYAPLSVEALSTVLTEEEERQNGCALIDFGASTTTLAVY
                     KNDVLQDLLVVPLGSKNITYDIAELGIEESNAERLKCLTGVAMEKFVEKHVYVQVAPS
                     SGTDPVRISTLFLAQIINARLDEMTEPVFKVLEELSFPLESGIIITGGGAKLKFLKEF
                     VEEKSGLKVRWGNHSDWLEDQAPDYLADSSYSQLIGTILLANENRRLHPLKSENTTKK
                     GQKNKKKITERITDGFLRFFDDETTWNK"
     gene            6469..7749
                     /gene="ftsZ"
                     /locus_tag="PLM04_05345"
     CDS             6469..7749
                     /gene="ftsZ"
                     /locus_tag="PLM04_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HON02534.1"
                     /translation="MINIEELVEMLPLELPEIENSIIKVIGVGGGGGNAVNHMYRQGI
                     TDVSFVVCNTDNQALVKSPVPLKIQLGANTTSGLGAGGKPEVARQAAEESIEKIQNIL
                     QDNTKMVFITAGMGGGTGTGASPVIAKAAHDMGILTVGIVTIPFAFEGNLKIRQALEG
                     VAALSEHVDAILVINNEKLKEIYPDLELSNAFAKADDVLTNAAKGIAEIITIPGYINT
                     DFADVYSIMKDGKVAIMNTGYASGENRITKAIENALNSPLLNTSDVSGASKVLLSLYC
                     SSTHQIKMEEVQQIHDFMRKVGENVQVIWGATFDDELEDRVKITLIATGYDVSDIPGM
                     PASVIKNKKRLETTPDKIEVFSELKPKENQPESKEKIIENAVNSYYGKSIPDEKPIEE
                     PELDLPVVNLEDLENDQTLKYIEKFPSWKRKKGK"
     gene            7801..8256
                     /locus_tag="PLM04_05350"
     CDS             7801..8256
                     /locus_tag="PLM04_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HON02535.1"
                     /translation="MEQLFEKVSADIKTAMLAHDKVTLEALRGIKKEFLEAKTAKGSD
                     GTLSDEAAIKILQKMVKQRKETAQIYKEQNRPDLEQNELAEIAVIEKYLPAQMSESEL
                     EATLREIIAQTGATGPQDLGKVMGVATRQLVGKADGKAISEMVKKLLSS"
     gene            8402..9055
                     /gene="nth"
                     /locus_tag="PLM04_05355"
     CDS             8402..9055
                     /gene="nth"
                     /locus_tag="PLM04_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HON02536.1"
                     /translation="MTTEERYLHILDWFMENMPNAQTELNYSNPFELLVAVILSAQCT
                     DKRVNMVTPQLFSDFPTPEAMASTTPEKLLEYIRSVSYPNSKAKHLVEMSRKLVADFE
                     GKVPDNVDKLQTLPGVGRKTANIIVSVIYNQPAMPVDTHVFRVSERLGLTTDSKNPLQ
                     TERELVRHIPPQLLPIAHHWLILHGRYVCHARKPKCNVCGLKSWCRYYFNHSLEQDI"
     gene            complement(9460..9834)
                     /locus_tag="PLM04_05360"
     CDS             complement(9460..9834)
                     /locus_tag="PLM04_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HON02537.1"
                     /translation="MEFNDAQAIYLQIADYISEHILLGKWKEGERLLSVRELGTELEV
                     NPNTVMRAYDFLQSNEIIYNKRGIGYFVADDAKEKILNYRRKRFLEKELPILFKNIHL
                     LNIDVEEIKKRYNDYLQKQQAL"
     gene            complement(9836..10603)
                     /locus_tag="PLM04_05365"
     CDS             complement(9836..10603)
                     /locus_tag="PLM04_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02538.1"
                     /translation="MNQKFSLKRIGLLLRRYFIENQNYELYLWSILTIVFMIFRNGNS
                     ASAILIVTGALYAVRFFKVFNFAPNGIQYLLIPATQLEKTISVFLLNTVYFILMFTVV
                     FSVGNVIGNLLEQLIFSTSDTVALQFFQSSKSFEFNIQGLKNIWISFGVFAAIQAIFT
                     LGSLYFKRNAILKTLLSLFVVAVALGILEYFIFKITFGSVTETLKALNAGDFVWDENI
                     FPKALTETWKIFWTISGYLFIPFCWIVSYFRLTEKEI"
     gene            complement(10616..11473)
                     /locus_tag="PLM04_05370"
     CDS             complement(10616..11473)
                     /locus_tag="PLM04_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON02539.1"
                     /translation="MIQIKHVEFSYSRNAQLFTGLELSLSPGHIHGILGKNGEGKSTL
                     LKMISGMIFPQKGEIEVLGYEPRKRQPEMLREIFFLPEELPPITLSIENYEKVYAPFY
                     PHFNHEQFQGYLQEFEINSGKSQINKLSYGQKKKVFMAFGLATNTKIVLLDEPTNGLD
                     IPSKAQFRKMTASALEEERIMLISTHQVRDLDSLIDSIIIMNSHQIVFNELCENITEK
                     LLFSYGKEKSEGEEVLYSEENLRGFDQVSINKTGEESKLDIELLFNTVLKNHQRIREI
                     FSNQNNKRF"
     gene            complement(12316..15642)
                     /gene="mfd"
                     /locus_tag="PLM04_05375"
     CDS             complement(12316..15642)
                     /gene="mfd"
                     /locus_tag="PLM04_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HON02540.1"
                     /translation="MLRVKEWANSLEQNLKISGLSGSSSALVFSSLFKAVPQHHIIIM
                     NDADEAGYLYNDLKLLLSENEIYFFPSSFKRAIKLSQLDAANDILRTEVLNRLANNSQ
                     PCIVVTYPDAIMQKIVSLQSLRQKTLKLHIGENIGIDFINELLFDFGFQRVDFVYEPG
                     QFSIRGGIVDIFSFANELPYRCDFFGDEIESIRVFDIETQLSKEEKQEIEIVPDLQRE
                     NKLPHVSFFEFIPKNTLLSFTHPNFIHDRINQLYDEFLVKANSEGHETPHLHKTMITG
                     DQWLELIEPFRKIDWGTDRTFFKTSSQISFNTSPQPVFHKNFDLISDHLRKNIEEGYR
                     IYIMSDSVKQTDRIAAIFADRGDNIPFQPIKSTLHEGFVDHDLSLLCYTDHQIFDRFH
                     KYQLSTDKTRQGKVVMTLKELNQLKIGDYMVHVDHGIGVFGGLVRTEVNGKMQEMVKL
                     IYKDDDILFVSIHSLHRISKYKGKEGESPHINKLGSGAWERLKERTKSKVKDIARELI
                     KLYAQRKAEEGFRFSPDTYLQQELEASFIYEDTPDQTKATVEIKKDMESTLPMDRLIC
                     GDVGFGKTEVAIRAAFKAVADNKQVAVLVPTTVLAVQHYNTFKDRLHDFPCNVEYLTR
                     ARSAKETKEILERLAKGEIDIIIGTHKLIGKSVKFKDLGLLIIDEEQKFGVAVKEKLK
                     QMKVNVDTLTMTATPIPRTLQFSLMGARDLSIISTPPPNRYPVQTEIHRFDEDVIREA
                     IQLEMNRNGQVFFVHNRIQNIYSIQAMIKRLVPGCRVAVGHGQMPPDELEEVIVDFIN
                     YDYDVLVATTIIESGIDIPNVNTIIINNAHTFGLSDLHQLRGRVGRSNRKAFCYLLTP
                     EFQLLTTEARHRLEAIETFADLGSGFNIAMQDLDIRGAGNLLGAEQSGFIADLGYETY
                     QKVLNEAVQELKDEEFADLYAEVEEKTTTGGTYVTDCQIDSDLELLFPPDYVENVSER
                     INLYRQLDSIENENQLAAFEKELEDRFGKIPPQGKSLILMVRLRWMAINLGIEKMVLK
                     NQQMTTYLVSNQKSHYYQSDTFGEILRYAASHPHQCQLREKNGRRSIIFLKVTSVEKA
                     WEILSEIYRNLETAKLQKAV"
     gene            complement(15731..16201)
                     /locus_tag="PLM04_05380"
     CDS             complement(15731..16201)
                     /locus_tag="PLM04_05380"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HON02541.1"
                     /translation="MEFQEKMMRKAIALSLENIKKGGGPFGAVIVKDGKIIATGVNRV
                     TSNNDPTAHAEITAIRKAAGKLKNFDLSGCEIYTSCEPCPMCLGAIYWAHLDKMYFAN
                     TKADAQNAGFDDSFIYEEINLKPEDRKLKSQQLLREEALAAFETWKNTTNKTEY"
     gene            16619..17674
                     /locus_tag="PLM04_05385"
     CDS             16619..17674
                     /locus_tag="PLM04_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HON02542.1"
                     /translation="MKKNFLPLLLLFLNLTVVFSQPKQGYYNQAVGKSGAELKTTLYS
                     IISSGYVSKSYDFLYTIYETSDVTPEGLIWDMYSDCTWVPGQKKCGNYSKVCDCYNRE
                     HSIPQSWFNSQSPMVSDAFHVYPTDGKVNGQRSNFPYGECSGGITLSGGRGRLGNSTF
                     TGYSGTVFEPDDEYKGDFARTYFYFATRYQNIMTTIDGDSFNKTTYPALSDWAKALFL
                     KWHRQDPVSKKETDRNNAVEVYQKNRNPFIDHPELAEYIWGTKVGEPWSLSSGIENLK
                     IDFSISPVPAQDFLNIKTDENELMCTIFNIQGQELMKTLIDGNSSISVSQLNNGMYLL
                     QLEKDGKKTIQKFMVSR"
     gene            17760..>19222
                     /locus_tag="PLM04_05390"
     CDS             17760..>19222
                     /locus_tag="PLM04_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HON02543.1"
                     /translation="MKKFFSTLFLILIVWLNCQIAFSEVPAGYYYLAHNKKKAELKSA
                     LSQIASPLRVLNYGSGKGYTWEGFYYADQNPDGSVKDMYSSEIRYFKDFSSVAGMHIE
                     HSLPKSWWGGHENNAYKDLFHLYPADGVTNSTKNNLPLGEVTGTPNFDNGVSKIGKNG
                     FGGIYTDNCFEPADEYKGDFARSYLYVSTVYENFATLWNSPMMNNNTYPVWKKWAIDL
                     LLKWNREDPVSQKERDRIEVVYNIQGNRNPFIDYPDLAEYIWGADTTKFFPFPEETEP
                     FLIYPKRGDQMDFGVILQTDSIQQHLTVSGLNLQNNLNIRLKGKSKSFKISKASLTKE
                     EVINGSEIIITFRPVVSGWNRDTLLLTSDGWKDSLRIPLQGLASTDFLALEPQNITPT
                     GGTLRWIFDPDADNYKLNLYQGDQQAGDLIISAYVEGSSWNKALELFNGTGKNIDLSN
                     YSLQKQSNGDGNFGATLKLNGMLENGKTYVLVHKSCS"
BASE COUNT         5826 a         3586 c         3930 g         5880 t
ORIGIN      
        1 tcaacgactc gaaagcgacc aatgtcaatt catgctggta tgcgctggaa agcatgaaaa
       61 cacccgttat tctgattttg ggcgggacgg acaaaggcaa cgattattcc gaaattgaaa
      121 gtctggtgaa agaaaaagtg aaagcattgg tttttttggg agtagacaac acgcctttac
      181 atcgtttttt cgatggaaaa atagataaaa tcgtggatgt ccgctcgatg gaagatgccg
      241 taagagcagc ttatgcaatg gccgaagaag gagataccgt tttgctatcg ccatgctgcg
      301 ccagctttga tttatttcag aattacgaag acagaggaag acaattcaaa acttgcgtaa
      361 ggaatctgtg atttatcaat tgtatatgtt tatttaagca aagagacaga tggaaaattt
      421 tctgaaaaaa tatttaaaag gaaatgcaac aatttggatt gtattcattt tgttgtgcct
      481 ggtttccatt cttgaaatgt acagtgcttc gagcacactg gcattcaaat cttccaatac
      541 ggccatgccc cttttgcgcc atgccggttt tttgctcttt ggcgttattt tggcatttct
      601 tgtccatctg attccataca aatatattcg attgctggct tatttgggac tgtttttgtc
      661 gttcatactc ctgattctgg tttattacaa gggtcaatcg gcaaacgacg caacacgttg
      721 gtttgaaatt ggcggactgc aatttcagcc ttcagaactg gcaaaacttt cggtcattat
      781 tgtagtggcg gattttatgt cgcgcatcaa aaatgaagca gcagacgaaa agaagtattt
      841 tcggtatatt ctcattgtac tggctgcaat atgtcctttg attctttttg aaaatttttc
      901 tactgccatg cttctttttg gtgtcgtatt ccttatgatg tttattggag gaatatccag
      961 aaaaaaattg ggattattgc tgctgtctat tacacttttt gtgttgggtg ggtacgctat
     1021 gattaaaacc gttcctgaag actcgatgcc caaaattttc gaccgtgcct atacatggtc
     1081 ggcacgcatc gaccgataca ccgaagaaaa gaaacaggaa gaaaacaaat atgtcattac
     1141 ggacgaaaac cggcaggtgc aacacggaag aatagctatt gcccgaggcg gaatgttggg
     1201 agtaatgccc ggcaacagtg tggaaaggga ttatcttcct caagcttatt ccgattttat
     1261 ttacgccatt attgtagaag aaatgggatt ggttggaggg gtaataatca ttctgtttta
     1321 tttggttcta ctttacagag ccggccgcat tgccgtaaaa agtgaaacgg tatttcctgc
     1381 cttgctagtc ataggacttt ccctgatgat agttttacag gcttttgtca gcatggctgt
     1441 agccacaagt ttgggccccg ttaccggtca gcctttgccg ttgataagcc gaggtggaac
     1501 ttccatcatt atcacctgca tatattttgg aattatttta ggcgtaactc gacaactgaa
     1561 aggagaacaa catgtcgaag aggaaatacc tgaagaacag gagcgcaatt ttcccgttgt
     1621 ggatttgaac gaattatcct aattttgtaa attgtttttt atacaacgtt ttagaaaatt
     1681 attgatgtaa atttcagttt tactgacaat tataatttaa aacaatgcct tatagattta
     1741 tcatcagcgg tggaggaaca ggaggtcata tcttccctgc catcagtatt gccaacgctc
     1801 tgaaaaagca gctgcccaat gcagaaatac tttttgtcgg agctttgggt cggatggaaa
     1861 tggaaagagt tcctgctgca ggttatccca tcaaaggttt acctgtaagc ggttttgacc
     1921 gaaaaaaccc gtttcgcaac gtcaaagtgc tgttgaatct tcggcgaagt ttacaaatgg
     1981 ctcgtcaaat cattcgcgat ttcaaacccg atgcagttat tggagttgga ggatacgcca
     2041 gtgccccgac cttgaaagct gcttccgatt tgggtattcc aacactgatt caggagcaga
     2101 attcgtatgc cggcattacc aacaaactgc tggcaaaaaa agctgcacgc atttgtgtgg
     2161 cttacgaagg aatggaacga tttttcccta aagaaaaact ggtattaacc ggcaatcccg
     2221 tacgtcagga actgctgtca atacccgaaa aaagtaaaga agcctttgaa ttttttggat
     2281 ttcgtccgga aaaaccaacc cttctggtag tcggaggaag tttgggagca cgaactatca
     2341 atcgcagcat aatatcgcat ttgcaggaac tttcccaagc cgatatccaa attatatggc
     2401 aaaccgggaa gttttatatt gaagaagcca ggaaagctgc cgaaccattt tcttcgcctc
     2461 aattaattgt tacagatttt gtttctcgga tggatttggc atattccgta gccgatttgg
     2521 ttatatcacg tgccggcgcc ggttcaattt ccgaattgtg tctgctgggg aagcccgcta
     2581 ttctggtacc ttcacccaat gtggccgaag atcatcagac aaaaaatgcg cttgcactgg
     2641 ttcagaagga agccgctgtg atgataaaag acgaagaggc cgaaaaaatg ctgataccga
     2701 aagcgttgga gttaataaaa gatgcggact cactcaataa actgggccaa aatatcagga
     2761 agctggcgca gaaagattct gccgaacgaa ttgcggcaga agtattgcaa ctgatttcaa
     2821 aaaagaattg attcataaca aatcgaatac gacctaaacc aattttaatt ttaaaaaatg
     2881 gattttacaa aatattattt cttgggtatt ggaggaatgg gaatgagcgc tttggctcgc
     2941 tatttcaatt ccaaaggatt tcaaactgcc ggttatgacc gcgttcagtc aaaagttact
     3001 gccgacttgc agacggaagg gatacaaatc aatttcgacc ccaatgtgga tcaaattccc
     3061 atctcgttca gatatcccga aaacactctg gtggtggtta ctccggctat ccctgatgat
     3121 ctgccacaac tcgtttattt ccgagaaaac ggatttacca ttaaaaaaag agctgaagtg
     3181 cttggcgaca ttacccgtaa agcttctacc atttgtatag ccggaacaca cggcaaaacg
     3241 acaacttcaa ctttgacagc tcatttgctt taccagtcgc atatttcctg caatgctttt
     3301 ttgggcggaa ttgccaataa ctacaactcc aatcttttgt tatcgaaaga tagcaatttg
     3361 ttcgtaatag aagctgatga atacgaccgt tcctttcatc aactctctcc ttatatggca
     3421 gttattactt ccgccgaccc cgaccatttg gatatctatc aaacgcctga agcttatcgc
     3481 gagagttttg agcatttcac gtcgctcatc agcccaggtg gcgttttgtt gatgaaaaag
     3541 ggagtcaata tccaaccgcg tctgcaaaaa ggagtaaagc aatataccta ctcaatgaat
     3601 gacggaggag acttttacgc tgaaaatatt catttggtga atcatgaatt ccatttcgat
     3661 tttgtaacac cttccgaacg catttcggat atacggttaa aaattccaat tcgcatcaac
     3721 atcgaaaata gtgttgctgc cattgcttta gcatggttga atggcgttac aaaagaagaa
     3781 atacgcacag caatagcctc ttattctggt atttatcgaa gatttaatct ggtttttcgt
     3841 tcggaacaaa caattttcat ggacgattat gcacatcacc caaaagaatt gaaagccagc
     3901 attttgtcca tcaaagaatt atttcccgaa aagaaaatta cgggaatatt tcaacctcat
     3961 ctctatagtc ggacgcgtga ttttgcttcc gattttgctg aagcattatc attgctggat
     4021 gagttgattc tgctggatat ttatccggca agagaagaac ccattgaagg aataagctct
     4081 gaaatgattt ttcgtaatgt taaactggac aataaaacac tatgttccag agaaaatcta
     4141 ttatctttgt taaaggacaa gcatccggaa gttttggtta cttttggagc cggcgacata
     4201 gataagctgg ttccgctcat taaaaaacag ctgatggagc aaaccaatgg aaatgcttga
     4261 tgaacaatat cttcccgttt tctgaaagaa agaaaaaaat cgaaattggg ttgaaacgta
     4321 taagaaaata tatttttcaa caagtaggaa gcgttcttaa attatgaaac tggaataaag
     4381 tcatgaaacc tgttttcaaa tatatattga taacattcgg tattctgctg gtaacaggat
     4441 atttggcttg ggcattgttg acttttcccg acccaaaagc aaacgaagtt tgcaacaggt
     4501 tccacattgt ttttttggat caatcaggcg ttaaacttat ctccgaaaaa gaagttgccg
     4561 aaatgatcga tatcaaaggt ttgaacccta tgggaaaaac gctcaaacat gtaaggacgg
     4621 aagaaataga agaattattg acaaaacatc ctttcatcaa acaagctgaa tgtttcaaaa
     4681 cgccatcagg tggagtgtat ttatttctgc aacagcgtca tccaaaattc agactggcag
     4741 gcaatgaaaa cttctatgtg gacgacgaaa aaaatataat tcctgtttcc tataacgatg
     4801 cagcacgtct ccctgtagtt tcgggattgg ttacaaaatc gatggcgaaa ggcgaattgt
     4861 tcgattttgt gtcttacttg gaagaaaatc ctttttggaa tgctcaaatc gaacaaattt
     4921 atattcgccc cgacagaaaa attgaacttg ttcctcgtgt tggaaccggc atcattttgc
     4981 tgggcaattt aaaagattat gagaaaaaac tccacaaact aaaaatactc tacgaaaaag
     5041 ggttcaatga atttggttgg aattattaca aaacaattga tcttcagtac gaaaatcaaa
     5101 ttgtctgcac aaaaaccgac aatattgatt gatgcaatgg ctgaagtttt ttgatatttt
     5161 tttaagataa atatttatga atcagaacac atatatagca cttgatttgg gaagttcgcg
     5221 tataactgct atggctgccg aagtattgga aagtggagcg gtgaaggtac ttgccacaga
     5281 atcgaaagca gccgatgatg tttatcacgg cattatcgaa cacacttcag gcgctgcttt
     5341 taaaattagc gaacttttga aacttttgca aaatagcgca aaaattcgcg atattcaaca
     5401 agtttgtgtg gctctgaatg ccaaaagcat gaaactttcc aaatcttcga ccactcgttt
     5461 tattggccag ggaaaaattg ttacagatgc tttgctggat gaaatgaaag aagaatgtga
     5521 acgaaaaaat tccgatgaaa acaacatagt gtttgatgta attccggttt cattcgaaat
     5581 tgacgggaaa attacaactg atcctgttgg acaatccggg aatcagatta caggctatta
     5641 tcatgttgta aaaggaaatg cacgcattct gcagcaattg aaaagctgtt tcgttcgcaa
     5701 tcaaattcag atcgaatatg cacctctttc agtggaagct ttatctaccg tactgacaga
     5761 agaagaggaa cggcagaatg gatgtgcgct gatagacttt ggggcttcca ctactacatt
     5821 ggccgtttac aagaatgatg ttttgcagga tttactggtc gttccgctcg gaagcaagaa
     5881 cataacctac gatattgcag aactcggcat tgaagaatca aatgccgaac gcctaaaatg
     5941 tttgacagga gtagcaatgg aaaaatttgt cgaaaagcat gtttatgttc aggttgctcc
     6001 ttcttccggc actgatccgg taagaatttc cacacttttt ctggctcaga ttatcaatgc
     6061 aagattggat gaaatgaccg aacctgtttt taaagtactg gaagagttgt cattcccact
     6121 ggaatcggga attatcataa ccggcggagg cgccaaactg aaatttttaa aggaatttgt
     6181 agaagaaaaa agcggtttga aggtacgttg gggcaatcac tccgactggt tggaagatca
     6241 agcacccgat tatttggccg attcatctta ttctcaactt attggcacta ttttactggc
     6301 aaacgaaaat cgacgtcttc atccgttaaa atcggaaaac acaacaaaaa aaggacaaaa
     6361 aaacaagaaa aaaattacag aaagaattac cgatggtttc ctcaggtttt tcgatgatga
     6421 aaccacttgg aataaatgag aaatttttaa tgaacattat aaattgatat gatcaatata
     6481 gaagaattag ttgaaatgtt gcctttggaa cttcccgaaa tagaaaattc catcatcaag
     6541 gtaataggcg taggcggtgg tggaggaaat gccgtcaacc acatgtatcg tcagggaatc
     6601 acggatgtgt cgttcgttgt ttgcaacacc gacaatcaag ctttggtaaa atcgcctgtt
     6661 cctctcaaaa ttcagttggg tgccaatacc acatcagggc ttggagccgg cggaaaacct
     6721 gaagttgcac gtcaggcagc cgaagaatcc atcgaaaaaa ttcaaaacat tttgcaggac
     6781 aatacaaaaa tggtatttat cactgccggt atgggtggag gcacaggaac aggagcttct
     6841 ccggtaattg ccaaagcagc tcacgatatg ggaatactta ctgttggtat cgttaccatt
     6901 ccttttgcct ttgaagggaa tctgaaaatc aggcaggcat tggaaggtgt agcagctttg
     6961 agcgaacatg tagatgctat tctggtaatc aacaacgaaa aattgaaaga aatctatccc
     7021 gacttggaac tgtccaatgc ttttgccaaa gcagacgacg tgctgaccaa tgcagcaaag
     7081 ggaatagcgg aaatcattac catccccggt tatatcaaca ccgactttgc cgatgtatac
     7141 agtatcatga aagatggcaa agtagccatt atgaacaccg gttatgcttc gggtgagaac
     7201 cgtatcacca aagccattga aaatgcgctt aactccccat tgctcaatac cagtgatgtg
     7261 agcggagctt ctaaagttct cttgagcttg tattgctctt cgactcatca aattaagatg
     7321 gaggaagtac aacaaatcca tgatttcatg cgaaaagttg gcgaaaacgt tcaagtaatc
     7381 tggggcgcaa cttttgatga cgaattggaa gatcgagtta aaataaccct tattgccact
     7441 ggttatgatg tgagcgatat acccggcatg cctgcatcgg ttattaaaaa taaaaaaagg
     7501 ttagagacta ctcctgacaa aattgaagtt ttctccgaat taaaacccaa agaaaatcaa
     7561 ccggaaagca aggaaaaaat tattgaaaat gccgtaaaca gctattatgg aaaatccatt
     7621 cccgatgaaa agcctataga agaaccggaa ttggacttgc cggtagtgaa cctggaagat
     7681 ttggaaaatg atcaaacttt gaaatacatt gaaaaatttc cttcttggaa gagaaaaaag
     7741 ggaaaataaa agtgtttttc cgagaattaa aaaagacaaa tctactaatt taaaatacaa
     7801 atggaacagc tatttgaaaa ggtaagtgcc gacataaaga ctgccatgct ggcgcacgat
     7861 aaagttacgt tggaagccct tcggggaatt aaaaaggaat ttctggaagc caagactgcc
     7921 aaaggaagtg atggaacatt atcggatgaa gcagccataa aaattttgca gaaaatggta
     7981 aagcagcgca aggaaacagc gcagatttac aaagaacaaa atcgtcccga tctcgagcaa
     8041 aatgaattgg cagaaattgc cgtcattgaa aaatatctgc cggctcaaat gagcgaaagc
     8101 gaactggaag caactctgag ggaaatcatt gctcaaaccg gtgccacagg gcctcaggat
     8161 ttgggaaaag taatgggagt tgccactcga cagctggtgg gaaaagcaga tgggaaagcg
     8221 atttcggaaa tggttaaaaa attgctttca tcataatata atttattttt ttaaggtctg
     8281 atggaaccca catgtctgaa tattatattc attcgtgttt gtgtatgcaa tacctgtggg
     8341 tttcatagtg ttttttgttt tcaatgaaaa gagaattttt ctaaactatt gattttattt
     8401 catgaccact gaagaacgtt atttgcacat tttagattgg tttatggaaa atatgcccaa
     8461 tgctcaaacg gagttaaact attctaatcc tttcgagtta ttggtagctg ttattttatc
     8521 ggcacaatgt actgataaac gcgtaaatat ggttacgcct caacttttct ccgattttcc
     8581 cacgccagaa gctatggcct caactacacc tgaaaagtta ttggaatata ttcgcagcgt
     8641 atcttatccc aacagtaaag ctaagcattt ggtggaaatg tcgcgaaaat tggtagctga
     8701 ctttgaggga aaagttccgg ataatgtgga caaactacaa acgcttccgg gtgtgggaag
     8761 aaagacagcc aatataattg tttcagtcat ctacaatcag cctgccatgc cagtcgatac
     8821 acacgtgttt cgggtttccg aacgcttggg actgacaaca gattcaaaaa atccattgca
     8881 gactgaacga gaattggttc gccacattcc gcctcaattg ttgcccattg ctcaccattg
     8941 gctaattttg catggtcggt atgtttgtca tgccagaaaa cctaaatgca atgtatgtgg
     9001 cttaaagtca tggtgccgtt attacttcaa tcattcatta gagcaagata tttaaaatga
     9061 gagaaaaatt tgaatcgaat tttactgttt ttgtcatctt ttctgaaaga gcctgatatt
     9121 tcagtattaa gctttaattt ttagcggtaa aagtttttgt ggcttcaaag aataagagcg
     9181 ttatatcttt gttttgaagt ttttcgaaaa tttttctttt ccaaaatctc gaatttacaa
     9241 cctgctttgg tttttttgtt tttgctgaaa aaaatactcc ctacaatttc gatgcatcat
     9301 ggctgcctaa accggtttct gtcttttgtt cctgtgttga ttcgaacatg cggtttattt
     9361 gactttttaa gattaaattg ttgataatca ttattataat tatatagata aaaaagcctg
     9421 ccagtaattt gttgctcgtc ttcatttctt taattgtttt cataatgctt gttgtttttg
     9481 aaggtagtca ttatatcttt tttttatttc ctcaacatcg atgttgagca gatgaatgtt
     9541 tttaaacaaa atcgggagtt ctttttccaa aaatcgtttt cgtcgataat tgagaatttt
     9601 ttcttttgcg tcatcagcca caaaatatcc gattcctctt ttgttgtaaa taatttcgtt
     9661 gctttgcaga aaatcgtaag ctcgcataac agtgttggga ttcacttcca attctgtacc
     9721 cagttccctt accgacagca atctttctcc ttctttccat ttgcccagca aaatgtgttc
     9781 gctgatataa tcggctattt gaaggtaaat agcttgtgca tcattaaatt ccattttaaa
     9841 tttctttttc tgttaaacgg aagtaactta ctatccagca aaagggtata aacaaatatc
     9901 ccgatatagt ccaaaatatt ttccatgtct ccgtaagtgc ttttgggaag atgttttcgt
     9961 cccatacaaa gtcaccggca ttcaaggcct tgagggtttc tgtaacggaa ccaaacgtaa
    10021 ttttgaaaat aaaatattcc aatatgccca atgctacggc tacaacaaac agactcagca
    10081 aggttttgag tatggcattt cgcttgaaat acagcgaccc caaagtgaaa atagcctgta
    10141 tggcggcaaa aacaccaaat gaaatccaaa tattttttaa gccttgaata ttaaactcaa
    10201 aactttttga ggattgaaaa aattgcaaag caaccgtatc gcttgtactg aaaataagtt
    10261 gttccagtaa atttccaatc acgtttccta ccgaaaaaac aaccgtgaac attaaaataa
    10321 agtaaacggt attgagcaaa aacacagaaa ttgttttttc cagttgtgta gccggaatca
    10381 gtagatattg aatgccattg ggtgcaaaat tgaaaacttt gaaaaatctt actgcataaa
    10441 gtgcacctgt aacaattaaa atggcgcttg cagaatttcc gtttctgaaa atcataaata
    10501 caatggtcaa aatgctccaa aggtagagtt cgtaattttg attttcgata aaatatcggc
    10561 gcaagagcaa tccgattctt ttaagactaa atttctgatt catattgaag aatttttaaa
    10621 aacgtttatt gttctgattc gaaaatattt ctctgatgcg ctggtgattt ttcagcacgg
    10681 tattaaacag cagttcaata tccagtttgc tttcttcacc ggttttgttg atactaacct
    10741 gatcaaagcc gcgcaaattt tcttctgaat aaagcacttc ttcaccttca cttttttctt
    10801 ttccgtaact gaaaagtaat ttttcggtaa tgttttcgca cagttcgttg aaaacaattt
    10861 ggtgagagtt cataatgata atgctgtcga tcaggctgtc caaatctcta acttgatggg
    10921 tggaaatcag catgatgcgt tcttcttcca aagcagaagc cgtcattttt ctgaattggg
    10981 ctttggaagg aatatccaaa ccattggtcg gctcgtcgag caatacaatt ttggtgttgg
    11041 tggccaatcc gaaagccata aaaactttct ttttctgtcc gtacgaaagt ttgttgattt
    11101 gcgattttcc cgaatttatc tcaaattcct gcaaatatcc ttgaaattgt tcgtgattga
    11161 aatgaggata aaagggagca taaacttttt cgtagttttc gatgctcaaa gttatgggcg
    11221 gcaattcttc gggcaggaaa aaaatctcac gcaacatttc gggctgacgt tttcggggtt
    11281 cgtacccgag aacttcaatt tctccctttt gtggaaaaat catgcctgaa atcattttca
    11341 acaaagtact tttgccttcc ccgttttttc ccagtatgcc gtgaatgtgg ccgggagaaa
    11401 gcgacagttc caaaccggta aacaattgag cgttgcggct gtagctgaat tcgacatgtt
    11461 tgatctgaat cataataaat tgtttattga attagtgtaa tagttaaata gtacaccaca
    11521 aaagtaattg cttttttata aagttgttca aatacataag ttaaattttg ttaattatca
    11581 aaattaattt gcaacaatat gattatcagc gtgtttaatt tttgaaaatt gagaagaaaa
    11641 accaaaaagt tttgaaatat ttcgtctcat tttttccaag tgcatcaact tgcgggcaga
    11701 gagataggtt tgttggcgag gaaatttgtt ctattccagt ttaaaacggt acaatgtttt
    11761 taccttcttt tttctttggt accagttgtt gaaaaccaat tcggcgtatc aacggaaaag
    11821 aaaccaaaat aatcgtttct gtgtggttcg agttgttcga aaagtttcat tttattttat
    11881 ttgctaaaat ccgataaatg attctttcaa ttttttctgc tctaaaatta caaaatttta
    11941 aaatatatga aagccggaag aaatgtacat tttgggagaa gaaaaaaatt atcggtttga
    12001 ttatcagttt gttttgttga caaaggcgaa aaactgcatt tttaacaaaa ttttctgtga
    12061 aaaagggcat caaatcgaga ataatgtttt atatttgcat taaagaaaaa cgagccgcat
    12121 tgtttagaga ctggaaaact catcagtaaa gaattcaaac cgagaaaagt ctgactttca
    12181 atggcgggct gcgccttcgg gtattcgtca gcaatgacga acggcagatt acaaagaaag
    12241 tcaacgtctc aaatcctatt caataggagt tttctcacaa caaacagtgc ggttttcgtt
    12301 ttttctaccc tttgtttata ccgccttttg taattttgcc gtttcgaggt tgcgataaat
    12361 ttctgaaaga atttcccaag ctttttctac cgatgttact ttgaggaaaa taatggatcg
    12421 gcgtccgttt ttttcacgga gttgacattg atgcggatgc gaagcagcat atctcagtat
    12481 ttcgccaaaa gtatcggatt gatagtaatg ggatttttga ttggaaacga gatacgtcgt
    12541 catttgttga tttttcagaa ccattttttc gatacccaaa tttatggcca tccatcgcaa
    12601 acgtaccatc aaaataaggc tttttccctg aggtggaatt ttaccaaagc ggtcttccag
    12661 ttccttttca aaagctgcca attggttttc gttttcaata ctgtcgagct gtcggtacag
    12721 attgatacgt tcggaaacat tttccacata atcgggagga aacagtaatt ccaaatcgga
    12781 atcaatctga caatcggtta cataagtacc accagttgtg gttttttctt ccacttcagc
    12841 atacaggtcg gcaaattctt catctttcag ttcttgaacg gcttcgttga gtaccttctg
    12901 ataagtttcg taaccgaggt cggctatgaa tccgctttgc tctgctccca acaaattccc
    12961 tgcgccacga atgtccaaat cctgcatggc aatattgaat ccgcttccca aatcggcaaa
    13021 agtttcaatg gcttcgagtc ggtgccgggc ttcggtagta agcaattgaa attcgggcgt
    13081 cagcagataa cagaaagctt ttcggttgct gcggcctact cttcctctga gttgatgcag
    13141 atcgctcagt ccgaaagtat gtgcattgtt gataataatg gtgttgacat tcgggatgtc
    13201 gataccggat tcgataatgg tagtggctac cagcacatca taatcgtaat tgataaaatc
    13261 gacaatgact tcttccaatt cgtcgggtgg catttgaccg tgtcctacgg caacgcggca
    13321 accgggtaca agccttttta tcatggcctg aatagaataa atgttctgta tgcgattgtg
    13381 tacaaaaaat acttgtccgt ttctgttcat ttccagctga atggcttcac gaattacatc
    13441 ttcgtcgaaa cggtgaattt cggtttgaac cggataacga ttgggcggcg gagtggaaat
    13501 gatggacagg tctctggctc ccatcagcga aaattgtaag gttcgaggga tgggtgtggc
    13561 agtcatggtt aaggtgtcca cattaacttt catctgtttc aatttttctt tcactgcaac
    13621 gccaaatttc tgttcctcgt cgataatcag caatccgaga tctttgaatt ttaccgattt
    13681 tccaatcagt ttatgggttc cgatgatgat gtcgatttca cctttggcca aacgttcgag
    13741 aatttctttt gtttcctttg ccgatcgagc tctggtgaga tattccacat tgcagggaaa
    13801 atcgtgtaat cggtctttga aagtgttgta gtgttgtaca gcgagcacgg ttgtcggaac
    13861 cagaacggca acttgcttgt tgtcggcaac tgccttgaaa gctgctctga tggctacttc
    13921 cgttttgcca aaaccaacat cgccacaaat caaccgatcc ataggcaaag tcgattccat
    13981 gtcttttttt atttcaaccg tagctttggt ttggtcgggt gtatcttcat aaataaatga
    14041 ggcttccaat tcctgttgaa gataagtgtc gggcgaaaac cgaaatcctt cttctgcttt
    14101 tcgttgagca taaagtttta tcagttcgcg tgctatatct ttaactttag atttggttct
    14161 ttctttcagt cgctcccaag ctcccgaacc caatttgttg atgtggggag attcaccttc
    14221 ttttcccttg tattttgaaa tgcgatgcag ggaatgaatg ctgacaaaaa gaatatcgtc
    14281 atccttgtaa atcaatttta ccatttcctg cattttcccg tttacttccg tgcgcaccag
    14341 gccgccaaaa actccgatgc cgtgatcgac atgtaccatg tagtcgccaa ttttcagctg
    14401 attgagctcc tttaaggtca ttaccacttt tccctgacgg gttttatcgg tacttaactg
    14461 gtatttgtga aaacggtcga aaatctgatg atcggtgtaa cacaaaagag aaaggtcgtg
    14521 atcgacaaaa ccttcgtgaa gagtggattt tatgggttga aaagggatat tgtcgcctct
    14581 atcggcaaaa atggcagcaa tgcgatccgt ttgtttcacg ctgtcgctca tgatgtaaat
    14641 gcggtaacct tcttcgatat tctttctcag atgatcgctt atcaaatcga aatttttatg
    14701 aaataccggc tgtggtgagg tattgaaaga aatttggctc gaagttttga aaaaagttct
    14761 atcggttccc cagtctattt ttctaaacgg ttcaatgagt tccagccact ggtctccggt
    14821 aatcattgtt ttgtgaagat gcggcgtttc atgtccttcg gaatttgctt ttaccaaaaa
    14881 ttcgtcataa agttgattta tgcggtcatg aataaaattt gggtgcgtga aactcagcag
    14941 ggtattttta ggaataaact caaaaaagga aacatgaggc aatttatttt cccgctgaag
    15001 atcggggaca atttcaattt cttgcttttc ctctttggac agttgggttt cgatatcgaa
    15061 aacccgaatg gattctattt cgtcgccaaa aaagtcgcat cgatagggca attcattggc
    15121 aaaggaaaaa atatctacaa ttcctcctct aatcgaaaat tgcccgggtt catatacgaa
    15181 gtcaacccgt tgaaaaccaa agtcgaacaa caattcgttg ataaagtcga taccgatatt
    15241 ttcgccaatg tgaagtttca aggttttttg acgcaaactt tgaagcgaaa caattttttg
    15301 catgatcgcg tcgggatagg taacaacaat gcacggctga gaattattgg caaggcggtt
    15361 cagtacttcg gttcttaaaa tatcgttggc agcgtcgagt tgcgacagtt tgatggctct
    15421 tttgaaagag gaagggaaaa aataaatctc gttttcagac agcaacagtt tcaggtcgtt
    15481 gtacagatat cctgcttcgt cggcatcatt cattattata atatggtgct gaggaacggc
    15541 tttgaacagt gacgaaaaaa cgagtgcaga tgacgaaccg ctcaaaccgg aaatttttaa
    15601 attctgttcg agtgaatttg cccattcttt tacccgcaac atctgcgggt gatgagcata
    15661 aagttgttga aattcagaga tattcaatat gcaaattaac tattataaat gcagttgcca
    15721 aaagcaactt tcagtattct gttttatttg ttgtgttttt ccatgtttca aatgctgcaa
    15781 gggcttcctc tctgagcagt tgttgcgatt ttaattttct gtcttcaggc ttcagattta
    15841 tttcttcgta aatgaaggaa tcgtcaaatc cggcattttg cgcgtcggct ttggtgttgg
    15901 caaaatacat tttgtcgaga tgagcccaat aaatagcacc cagacacatt ggacagggtt
    15961 cgcacgaagt ataaatttcg caacccgaca ggtcaaaatt tttcagtttg ccggctgctt
    16021 ttctgatggc cgttatttcg gcatgtgcag tgggatcgtt gttggatgta acacggttga
    16081 cgccggtagc aatgattttc ccatctttca cgattaccgc accaaacggg cctcctcctt
    16141 tttttatatt ctccaaagac aatgcaattg ctttacgcat cattttttct tgaaactcca
    16201 tgtcgttgac gtattctaca gtttcttttt ggttcttgaa acatcaatac aaagataata
    16261 ttttttcagt aaacaggtta tacctgctag ttacatacta ttttttgaat aaacctttta
    16321 aaaaaaaatt cggattaaat attcacgtta ttttggtcaa ttttagctga atgtacctga
    16381 attataggtt ttaatttttc cttagaatga atttttcaaa cattttatta atttcacatt
    16441 tgtcgattaa ctctacagcc ttctattgtc tttttttaac ttgccattta aaaataatgt
    16501 tttatctttg aagccctaaa tttttaggaa attttataaa tattcatttt ttaaacattt
    16561 ttatttgttt attgcataaa aactcattta ctcatttgtt taattgaatt aattcatcat
    16621 gaagaagaac tttctcccat tgttgttgct ttttttgaat ctgacagttg ttttttctca
    16681 acccaagcag ggttattaca atcaggccgt aggaaaaagc ggtgccgaac tcaaaaccac
    16741 tctttattcc attatttcat caggttatgt aagcaaaagt tatgattttc tgtacaccat
    16801 ttacgaaacc agcgatgtta ctcccgaagg tcttatttgg gacatgtatt ccgactgcac
    16861 ttgggtgccc ggacaaaaaa agtgcgggaa ttatagcaaa gtttgcgatt gttacaaccg
    16921 cgaacattcc ataccgcaaa gctggtttaa ttctcaatcg cctatggtaa gcgatgcttt
    16981 tcatgtttat cccaccgatg ggaaagtgaa tgggcagcga agcaatttcc cttatggcga
    17041 atgtagcggt ggaattaccc tttccggtgg aagaggaaga ttgggaaatt ccacttttac
    17101 aggatattcg ggaacagttt ttgaacccga tgatgaatat aaaggcgatt ttgcacgtac
    17161 ttatttttat ttcgccacac gttatcagaa cattatgact accattgatg gagattcatt
    17221 taataaaaca acttatccgg cgctctcgga ttgggcgaaa gcattatttt tgaaatggca
    17281 ccggcaggat ccggtttcaa aaaaggaaac cgacagaaac aatgccgtag aagtttatca
    17341 aaaaaacagg aatcctttta ttgatcatcc ggagttggca gaatatattt ggggcacgaa
    17401 agttggtgaa ccttggtcgc tttcttcagg aattgaaaat ctgaaaatag atttttccat
    17461 aagtcctgtg ccggctcaag attttctgaa tatcaagact gacgaaaacg aattaatgtg
    17521 tactattttc aacattcagg ggcaggaatt aatgaaaacc ttgattgatg gaaattcttc
    17581 tatatctgta tctcaactaa ataacggaat gtatttgctg caactagaga aagatgggaa
    17641 aaaaaccatt caaaaattta tggtcagcag gtaatggcag caacttttat attttacaaa
    17701 atagtgatga taaataagga tgatcttaaa aacgtctcgc atttaatttg aacgaaaaaa
    17761 tgaaaaaatt tttcagcacg ctttttttaa ttttaattgt ttggctaaac tgtcagattg
    17821 cattttcgga agtgccggct ggttattatt atttagccca caacaaaaag aaggcagaat
    17881 taaaatcagc tctttctcaa attgcatctc cattgcgtgt cctcaattat ggcagtggga
    17941 aaggatatac atgggaaggg ttttattatg ccgatcaaaa cccggatggt tcggtaaaag
    18001 atatgtattc ttcggaaata cggtacttca aggatttttc ttccgtcgca ggcatgcata
    18061 ttgagcactc attgccaaaa agttggtggg gcggacacga aaataatgca tacaaggatt
    18121 tgtttcattt gtatccggcc gatggagtta ccaattctac aaaaaacaat cttccgcttg
    18181 gggaagttac cggcacgcca aatttcgaca atggggtaag caaaattggg aaaaatggtt
    18241 ttggaggaat ttataccgac aattgctttg agcctgccga tgaatataaa ggcgattttg
    18301 cgcgttctta tctgtacgtg tcaactgttt acgaaaattt tgctaccttg tggaattcgc
    18361 ccatgatgaa taacaacact tatccggtgt ggaaaaaatg ggctatcgat ttattgctga
    18421 aatggaatag agaagaccct gtcagtcaaa aagaacgcga tcgtattgaa gttgtttaca
    18481 acattcaggg aaatcgcaat ccttttattg attatcccga tttggcagaa tatatttggg
    18541 gtgccgatac aacaaagttt tttccttttc ctgaagaaac agagccgttt ctgatttatc
    18601 ccaaacgcgg tgatcaaatg gatttcggcg taatattgca aaccgattcc attcaacagc
    18661 atttaactgt ttcaggattg aatttacaaa acaatttgaa tatcagactt aagggcaaaa
    18721 gtaaatcctt taaaatttcc aaagcttcgt tgacgaaaga agaagttatc aatggcagtg
    18781 aaataattat cacttttcgt cctgtggttt caggctggaa cagagatacg cttttgctga
    18841 cttccgacgg ttggaaagat tctttgcgta tcccactgca aggtttggct tcgactgatt
    18901 ttcttgcctt agaaccgcaa aatatcactc ctacgggagg aacattacga tggattttcg
    18961 accccgatgc cgacaactac aaactgaatc tgtatcaagg cgatcagcaa gccggcgatt
    19021 tgattatttc cgcttatgtg gaaggcagta gctggaacaa agctttggaa ttgttcaatg
    19081 gaaccgggaa aaatatcgat ttatccaatt acagtctcca aaaacaatcc aatggagatg
    19141 gaaattttgg agctacactg aaattgaatg gaatgctgga aaacggaaaa acttatgtgt
    19201 tggttcacaa aagctgttca aa
//
LOCUS       DAOTAX010000038        19014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2389_length_19014_cov_31.327074, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000038 DAOTAX010000000
VERSION     DAOTAX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2389_length_19014_cov_31.327074"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..2750)
                     /locus_tag="PLM04_05395"
     CDS             complement(75..2750)
                     /locus_tag="PLM04_05395"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HON02544.1"
                     /translation="MKIHFKIHFITQWGQSIVLLVKNDNHDTPQRIEMNCGKNFEWTA
                     VIDIQPGTKELFYRYALENDSSTLEFEYGDFRQIKIDTEFSDVYISDNWRARYGDSPF
                     ESSAFTDCLFKRNSLYAAPQVKDANLVLRLRCAQLEPNRHFAVIGNQDALGNWDVAKK
                     IRLDESDFPVCSVALNATKFKFPLEYKYLIVDTATDEVLAWGGGPNRVVESVMKKSLN
                     IVNDEHFIRTIPSWKGAGVAIPVFSLRSEESCGIGEFYDLKKMVDWAKKTGQRIIQTL
                     PINDTILYHTNYDSYPYNAVSVYALHPIYLHLESMGTLNDELQKKYFEDKKKEFNAKT
                     FSDYQNVMNLKWEYFEVIYQQEKATVFASESYLNFFVSNKSWLIPYAAFSFLRDFYGT
                     PDFRRWEKYSRYEEQEILEFSDPKQPHYDQLAIYYYLQYHLHCQLSEVRQWAHENGVV
                     IKGDIPIGVSPFSVDVWINPELFNTQVQAGAPPDDFSLTGQNWGFPTYNWEEMEKDDF
                     LWWKKRFQKLSEYFDAYRIDHILGFFRIWEIPADDVWGLTGSFHPALPFTRQELEAKG
                     LWWDESRYLEPYLKEHVLYATFGKYADDVIREFFVPHGWKSYKFKPEYDTQKKVEAYF
                     NSLGYNFSNKDVMIRDGLYSLHCEVLFVRDLRQPEKFHPRIAMHSTASFRDLPDETRR
                     TLDRIYVDFFYHRHNEFWKQQAMKKLPALITATSMLPCGEDLGMVPDSVPEVMQILEI
                     LSLEVQRMPKKPNMEFGMPSEAPYLSVCTTSTHDMNPLRAWWEEDSAVTQRFYNHALG
                     LQGAAPKTCETWIVERIVQQHLQSKAMWVILPWQDWMALDEKLRYEHPFAERINVPSN
                     PRNFWCYRMHLTLEKLLSEDDFNQYLFQLISQSGR"
     gene            complement(2814..3695)
                     /locus_tag="PLM04_05400"
     CDS             complement(2814..3695)
                     /locus_tag="PLM04_05400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON02545.1"
                     /translation="MSIKHLEHSEIDYEKWDKSISQAKNQLVYAETWYLDIVSPEWEA
                     LVADDYKYVMPLPVKTKYAIPYLVQPILTQQLGIFSASEVSEIIIKEFLKRIPYPSYE
                     LNFNEENFIPEAESFPNYVLSLRPTYEELKTKFSKNTKRNIDKAQQAGLQVRNLFSIS
                     DFLDFYYLQEKKFIYPERTLLEILLGQGKEKNAVDIFGVFSPHNELIAALCMIHSKKR
                     LTYWIPVSNAVGKKTSAMFLLVDEIIRQNAERDVILDFEGSRVDGIARFYKGFGSEYK
                     PYFVLKRFRPSFLVGKI"
     gene            complement(3963..4736)
                     /locus_tag="PLM04_05405"
     CDS             complement(3963..4736)
                     /locus_tag="PLM04_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HON02546.1"
                     /translation="MKITLIQDVIAWSDKQANFNRINRHLETLQGETDLVVLPEMFST
                     GFCTNRLDLAEEGKGETVQTLSNWAKNFGIALTGSFIACENNRYFNRSFFVFPDGNIQ
                     TADKRHLFRHGGEHQYFSAGNRHLLVHYKKWNICVLVCYDIRFPVWSRNVDNRYDLLI
                     YTANFPSKRIRDWDILLQARAIENQAYVCGLNRIGTDGQGIEYNGHSTLVDFKGRFLL
                     TFPENIFDIKTAEISLDSLQNYRQHFPVWMDADRFQIIE"
     gene            complement(4775..5323)
                     /locus_tag="PLM04_05410"
     CDS             complement(4775..5323)
                     /locus_tag="PLM04_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HON02547.1"
                     /translation="MKKLIYLFFKSVSWEIIVPHEIPEKSVICFAPHTSNCDFVIGKL
                     FSWSVGMQFSFLVKKSWFVFPLGYFFKAVGGIPIDRSKSNATVEQMIAEFEKHDRFHL
                     VIAPEGTRSYVPAWKKGFYYIASGANVPIELAFIDYKKKQIGIGKLLYPSGNFSSDFS
                     EIQQFYQNISPRYPEKYNPTIF"
     gene            5900..6355
                     /locus_tag="PLM04_05415"
     CDS             5900..6355
                     /locus_tag="PLM04_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HON02548.1"
                     /translation="MEKIDELDRQILKIITQNARLPFRDVAVQCGVSRAAIHQRVQRL
                     IDMNVITGSGYFVNPKKLGYQMCVYIGITLERGSMYKEVVQELEKIPEIVESQYTLGP
                     YTILVKLYAKNDEHLMELLNGKIQEIKGVATTETLTSLDQRIRRTIPID"
     gene            6409..7437
                     /locus_tag="PLM04_05420"
     CDS             6409..7437
                     /locus_tag="PLM04_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HON02549.1"
                     /translation="MFAFKVIHAAFYFFADMKVLVTGATGLLGHQVTMELIQHGYQVQ
                     IIVRKMDNIFFDLNAVKVFIGNFTEYEQLKAAASGCEAVIHIAAVTDVHLLHYEDYRK
                     INVEGSAQVIRVCRDLNIRNVVYISSSNTIGFGSKSKPATEDFPFQYPFTRSFYARSK
                     VEAEKLFQQFASEFGNHVIIIHPTFMIGAYDVKPSSGKLLLMGYRKLLLFIPKGGKNF
                     VPVRDVAKAVCNALTLGKNGEHYLTAGVNLSFAEYYRLQKNIAGYRQIIMEVPDFLLK
                     VLGKVGDFLRFLGIKTDVCSMNLSQLMIREYYCGQKAIDELNMPQTDLKFAIAEAVEW
                     FKERGWIR"
     gene            complement(7484..>10365)
                     /locus_tag="PLM04_05425"
     CDS             complement(7484..>10365)
                     /locus_tag="PLM04_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HON02550.1"
                     /translation="VLSQAVDNLFFNLSKNENKLLLDWLISFAEESMEQNGKWNLKND
                     IINFGAEIFKENYQYRAEEIGKKLHNLDFLGKYIGKLKKIKTDFEEEVKKLAQDGIRI
                     MTDFELSHDDFSYKETKLFDKLISGNVAAPNSRFRKYADDVENCTNKSTPNWVKNKVN
                     SAYISGLGKCFEKINEIFQEPFIRYNSVDIVLKHIYKLGILSDLAVEINQLTAEQNTF
                     LISDTNLLLNRIIDNSDTPYVYEKIGNKLQHFMIDEFQDTSVLQWKNFLPLVRNSLAE
                     NNFNMVVGDVKQSIYRWRNSDWKLLDEQIFKDFTPQQIRQEPLNVNWRSDRNIVKFNN
                     NFFSQSAQLLQQQLNANIQEYLPADKYPFVSELSNKIQHAYTDTEQKVSEKAGAGHVC
                     IQFIPNDEKNWQEESLNRLPIMLENFQDRGYKASDIAILVREKKDAALVIQSLMNYKN
                     SPSAKEGYCYDILGNEGLLIGKASSVRFIVGLLKLFVSPSDDIQLTIVSLEYALNKLG
                     KTKNEALRDIAALKENTENKFSHLFDCEEEKALQAIRHYSLFDRVEQIISLFNIGEWE
                     NQAPFVQAFQDLVYNFTAKGFSDISDFLSWWDEAGCNKFVAMPADQEAIHIMTIHKAK
                     GLDFPVVILPLCDWALEGNIHKTNVLWCETNVPPFSELPLLPISYNQYLRQSIFTQNY
                     FDEQMHLYIDNLNLAYVAFTRPKNELVCMAPAPDEKNEEKKKKSTSETVKNVSSLLWQ
                     FVQTQQSTIGENSSMRKDDENKIYEWGEATYAPQKEIISSTKSEKFVQYPSCNSAQKL
                     QIRHQGTQYLIEEKDLSDSQLNYGNIMHEILRKITNQADQDKVLLEFVSSGQISEAEK
                     ITVEKGLEKFWSLPQAKEWFNGEGRILNETTILSPENRLHRPDRVIIQEAKATVIDYK
                     FGQKESKYHLAQVKEYMDLIAQMGYQTKGYVCYAELGKVVEVFQN"
     assembly_gap    10366..10465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10466..10926)
                     /locus_tag="PLM04_05430"
     CDS             complement(<10466..10926)
                     /locus_tag="PLM04_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HON02551.1"
                     /translation="MLNVYRASAGSGKTFQLTKDYIHLLFRHQGENLHRHILAVTFTN
                     KATEEMKSRILKELFALAQGQPSNYRKSLMEEYARNEAEVNLKAKQILVSLLYDYSSF
                     AVSTIDSFFQQVIRSFAREMGINGAYNLEMENDAVLSQAVDNLFFNLSKNE"
     gene            complement(10965..11297)
                     /locus_tag="PLM04_05435"
     CDS             complement(10965..11297)
                     /locus_tag="PLM04_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HON02552.1"
                     /translation="MDWKEKLNQLKDSLPQDNSEPTENELPEKKVQTEPLRVQMEKRN
                     GKPVTIVSEFEGNEKELKELAKTLKVSCSAGGTAEHGMVLIQGDFRVKIAEILQKMGY
                     KVKKINFK"
     gene            11829..12641
                     /locus_tag="PLM04_05440"
     CDS             11829..12641
                     /locus_tag="PLM04_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HON02553.1"
                     /translation="MKEVTKVLYISQEIFPYLPASEMANMGRYLPQAVQEKGLEVRIF
                     MPKFGSINERRNQLHEVIRLSGMNIIIDDTDHPLIIKVASIPAARMQVYFIDNEDYFQ
                     RKNTVANENNEEYDDNDERAIFFARGVMETVKKLRWSPDVIHCQGWITALVPLYVKKI
                     YNDDPFFKHSRVVYSIFDDEFKKPLNDRLMDKILMDGIQKKHLASIKNPPVDYHSLLK
                     LAVDYSDGFIQSSPKVNESVLKYVADKKIKFLPYQPAEDYSDQYIDFYNQLF"
     gene            12672..14042
                     /locus_tag="PLM04_05445"
     CDS             12672..14042
                     /locus_tag="PLM04_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HON02554.1"
                     /translation="MHFNKFFLFLFTLAFVSCTDGLTDIGVSIMPSQDIISVNADAFD
                     LNTENVFVDFIYTRQDSFLLGNFYDHKYGSTRADILAQVNCPVDFTFPDNAVPDSAVI
                     ALRYLTWFGDKYSPMEIKIYQMNKATFSYSEHYPSNINPDDYTDRSILLGKRIITPSD
                     TSGSRSDGTQVRFKLSDSFVQWFFPENRTYNSQKDFLQKFKGMFITTTFGSANILYVN
                     QIDLYYYYHYTYVRKTSDGSDSTVTVNNVITFPANAEVRQVNRIQHPDRNEVVQSRPE
                     VNYVASPANINTRISLPVDKIAKQMKTTIHNKYLSVNSALLKVEVTEQDEATIAVPLV
                     QNMMLIKESALDRFFKNSELPSDTCALIAQYSYTKNADTNSYDYYYTFNLSKLIANEI
                     KKAETNGTQLPETMDLLLVPVTVQYNNSGYYTSVKHQILLSGVTIKSGKNTSSPMKIN
                     LVYSGF"
     gene            complement(14056..15369)
                     /gene="der"
                     /locus_tag="PLM04_05450"
     CDS             complement(14056..15369)
                     /gene="der"
                     /locus_tag="PLM04_05450"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HON02555.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLTQTRRAIVNETAGTTRDRQYGK
                     SEWSGYEFSVIDTGGWVTNSSDTFEQEIKRQVQLAINEADVILFLVDVQCGITDLDFE
                     VAQLLKKTNKPVILVTNKADTFEWQYQAPEFYKLGMGDPFLVSSINGLGTGDLLDKLV
                     SYLKPQEDEETEEHLPRFAIVGRPNAGKSSLLNSFLDEERNIVTEIPGTTRDSIYTRF
                     NKFGFDFYLVDTAGIRKKAKVDEDLEYYSIVRSIRAIENSDVCMLLIDATRGLESQDL
                     NIFSIIQKNKKGIVVCVNKWDLIEDKDTHSTKKMEAYIRERMSPFTDFPIIFISALTR
                     QRIFKVLESAVKVYENKNKKIPTAKLNEYLLPLIQNFPPPALKGKYIKIKYVTQLPDT
                     QVPTFIFFANLPQYIKEPYRRFLENKIRSQWDFTGTPIQLFFRKK"
     gene            complement(15582..17525)
                     /gene="thrS"
                     /locus_tag="PLM04_05455"
     CDS             complement(15582..17525)
                     /gene="thrS"
                     /locus_tag="PLM04_05455"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HON02556.1"
                     /translation="MIKITFPDGSVKEFPKGITGLEIAESISSRLAQDALSITVNGQV
                     WDLTRPIETDAAVKINTWEDEEGKHAFWHTSAHLMAEALQELYPGIKFGIGPAIENGF
                     YYDVDPGEAVIRESDLPAIEAKMVELAARGEKLKRTDISKADALKKFQTKGDEYKVEL
                     INDLQDGTITLYSQGNFTDLCRGPHLPNTEKIKAIKLLSVAGAYWRGDEKRKQLTRIY
                     GITFPKKKMLDEYLLLLEEAKKRDHRKIGKELELFMFSEMVGKGLPIWLPRGTDLRLR
                     LENVLKTIQQRFEYQQVITPHIGNKQLYVTSGHYAKYGKDSFQPIHTPEEGEEYLLKP
                     MNCPHHCEIYKFKPRSYKDLPLRFAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PDQVKDEFLKVMDIIFIIFNALDFKNFEAQISLRDPDNKEKYIGTDENWEKAEKAIIE
                     ACEEKGLKAKIVQGEAAFYGPKLDFMVKDAIGRKWQLGTIQVDYNLPERFELEYTGED
                     NQKHRPVMIHRAPFGSMERFIAVLIEHTAGKFPLWLTPDQVVVLPISEKFNDYAFDIA
                     KKLKEKSIRVTVDERNEKIGRKIRDNELKRIPYMLIVGEKEMETSHVSVRRQGEGDAG
                     SMSVEEFAELIHNEVVSQMKQFN"
     gene            complement(17988..18944)
                     /locus_tag="PLM04_05460"
     CDS             complement(17988..18944)
                     /locus_tag="PLM04_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02557.1"
                     /translation="MSEKDLFGFVIPAPPKIEVNIYADETMNRKCSDTKHNWHYMCLI
                     FERVDKPLLDDLIIERYCNNFDSASPYYFKNNKILHWTEMKNADEINICKRWFQYILN
                     PEKSSDKFYCYILGLNESYLSDDEFDADNKFNSIYNRFFRTAIEYSLKCFWGNNEIVI
                     KNIFHEQGQQQDHAYFSWHPIDKLQNKKNNFKFETDKIQFLGKDHKIDEKANILQLCD
                     CFLGAIVNLIHGIENPESKKSRSKNELLDLLLPLCQRMTKNPDNPNSSYRHKNRIRIQ
                     FFPKNKCEKGDIRRFQNQFYSDRPLKYGIDKDDSNQLSLNFN"
BASE COUNT         5497 a         3691 c         3514 g         6212 t
ORIGIN      
        1 ttttatcgca atatccaata aaaaagagac tgtctctact tttgagacag cctctattac
       61 tgaaaaacaa tttcttatct tcccgattgg ctaatcagtt gaaataaata ttgattgaaa
      121 tcatcttcac tcagcaattt ttcgagtgtc aaatgcattc ggtaacacca aaaatttcgt
      181 ggattgctgg gtacgttgat gcgttcagca aaaggatgtt catagcgaag tttttcgtcc
      241 aaagccatcc aatcctgcca cggcaaaatt acccacatag ctttcgattg aagatgctgt
      301 tgaacaatgc gttcaacaat ccatgtttca caagtttttg gagctgctcc ctgcagaccc
      361 agcgcatgat tgtaaaaacg ttgagttacg gcggaatctt cctcccacca tgctctgagt
      421 ggattcatat catgagtaga agtggtacat accgataaat agggagcttc cgatggcatt
      481 ccaaattcca tattgggttt ttttggcatg cgttgaactt caagactcaa aatttcgagt
      541 atttgcatta cttcgggcac agaatcaggc accataccca aatcctctcc acacggtaac
      601 atactggtag cagtaataag ggctggtaat ttcttcattg cctgctgttt ccaaaattcg
      661 ttatgacgat gatagaaaaa atcgacgtaa attctatcca gcgttcgacg cgtttcatcc
      721 ggcaaatcgc gaaaggaagc agtactgtgc atggcaatac gcggatgaaa tttttccggt
      781 tgacgtaaat cgcgcacaaa aagaacttcg caatgcaaag agtataaacc gtcgcgaatc
      841 atcacatctt tattgctaaa attatatccc aaagaattga aataagcttc aacttttttc
      901 tgcgtatcat attcaggttt gaacttataa cttttccagc catgaggtac aaaaaattcg
      961 cgaatgacat catcggcata ttttccaaaa gtggcataca acacatgttc tttcaaataa
     1021 ggttccaagt aacgactttc gtcccaccaa agtcccttag cttcaagttc ctgacgcgta
     1081 aaaggcaaag caggatgaaa actgccggta agtccccaga cgtcatctgc cggaatttcc
     1141 cagatacgaa aaaatccgag aatatgatcg atacgataag catcgaaata ctccgaaagt
     1201 ttttggaaac gctttttcca ccaaagaaaa tcatcttttt ccatttcttc ccaattgtaa
     1261 gtgggaaaac cccaattttg acccgtaagt gaaaaatcat caggtggagc accggcctgt
     1321 acctgcgtat taaacaattc cggattaatc cacacatcca cactgaaagg gctcactcca
     1381 ataggaatat cacctttgat aactacacca ttttcatgtg cccattgcct tacttcggac
     1441 aactgacaat gcaaatgata ttgcaaataa taatatatgg caagttgatc ataatgtggc
     1501 tgttttggat cggaaaactc caatatttcc tgttcttcgt accggctgta tttctcccat
     1561 cgtctgaaat cgggcgttcc ataaaaatca cgtaaaaaag aaaatgcagc ataaggaatc
     1621 aaccaagatt tattggagac aaaaaaattc aaataggatt cggaagcaaa gactgtggct
     1681 ttttcctgtt gataaataac ttcaaaatac tcccatttaa gattcatcac attttgataa
     1741 tcggaaaaag ttttggcatt aaattctttt ttcttatcct caaaatattt tttctgcaat
     1801 tcatcattta aggttcccat actttccaga tgaagatata tgggatggag cgcataaacc
     1861 gaaacagcat tgtaaggata cgaatcgtaa ttggtatgat aaagtatggt atcgttaatt
     1921 ggcaaagttt gtatgatacg ctgaccggtt ttttttgccc aatctaccat tttttttaaa
     1981 tcgtaaaatt cacctattcc gcaactttcc tcactgcgca gcgaaaaaac aggtatggcc
     2041 acgccggcac ctttccatga aggtatcgta cgaataaaat gttcatcatt aacgatattt
     2101 agcgatttct tcatcaccga ttccaccacc ctgtttggtc cgccacccca tgccagcact
     2161 tcatccgtag ccgtatcaac tattaaatat ttatattcga gcggaaattt gaattttgtg
     2221 gcattcaacg caacgctgca caccggaaaa tcagactcgt ccagacgaat tttctttgca
     2281 acatcccagt tccccaatgc atcctgattg ccaataacag caaaatgccg atttggttca
     2341 agctgagcac atcgcagacg aagaaccaga tttgcatctt tgacctgtgg ggctgcatac
     2401 aatgaatttc ttttgaaaag gcaatcggta aaagccgaag attcaaacgg actatcgccg
     2461 taacgtgctc gccaattatc gctgatgtat acgtcggaaa attccgtgtc gatttttatt
     2521 tgacgaaaat cgccatattc aaattccaat gtagatgaat cgttttccaa agcatagcgg
     2581 taaaaaagtt cttttgtgcc cggctgaata tcgataacgg ctgtccattc aaaattttta
     2641 ccgcaattca tttctattct ttgcggtgta tcatgattgt cgttctttac caaaagaaca
     2701 atcgattgcc cccactgcgt aatgaaatgt attttaaaat gaattttcat gtgttttatt
     2761 tctgttgtcg atcagtgtat ttattctgtt aacaggtcat tagtaaaagc aaattaaatt
     2821 ttccctacta aaaaagaagg tctgaaacgt ttcaaaacaa aataaggctt gtattccgaa
     2881 ccaaaacctt tgtaaaatct tgctatccca tcaactcgtg aaccttcaaa atcgagaatg
     2941 acatctcttt cggcattttg tcgaataatt tcatccacca gcaaaaacat ggcagatgtt
     3001 tttttgccaa cagcattcga aaccggaatc caataggtta accttttttt ggaatgaatc
     3061 atgcagagag cagcaatcaa ttcgttatgc ggagaaaaaa cgccaaaaat atcaacagca
     3121 tttttttctt tcccttgccc gagaagaatt tcgagcaaag tacgctccgg ataaataaat
     3181 tttttctcct gtaaatagta aaaatctaaa aaatcgctga tagaaaataa atttctcacc
     3241 tgcaaaccgg cttgctgcgc tttgtctata tttcttttgg tgttttttga aaatttagtt
     3301 ttgagttcct catacgttgg ccgaagcgac aatacatagt tcggaaaaga ctcggcttcg
     3361 ggaataaaat tttcttcatt aaaattcaat tcataacttg gatatggaat tcttttcaga
     3421 aactctttaa taattatttc gcttacttct gaagcagaaa aaatacccag ttgttgcgtc
     3481 aaaatcggct gaacaagata gggaatggca tattttgttt ttaccggcaa aggcataaca
     3541 tatttgtagt catctgccac aagtgcttcc cattccggag atacaatatc caaataccat
     3601 gtttcggcat aaaccaactg atttttcgct tgcgaaatgg atttatccca cttttcataa
     3661 tcaatttcgc tatgttcgag atgctttatg gacataaaaa atttgtttaa caaaagtaaa
     3721 aacaaactta cataaaaaaa atgatttata cactcctcac gataaaaatc atttgtattc
     3781 agtcaatcct taaaaagtca atttttatgc agaaagtcta taaaactttt tgaattggac
     3841 tcaatatttt gtttttcatc agaatataat ttatttttaa gggtctgatg gaacccacat
     3901 gtctgaatat tatattcatt cgtgtttgtg tatgcaatac atgtgggttt cataaaaagt
     3961 tattattcaa ttatttgaaa tcggtcggca tccatccaca ccggaaaatg ttgacggtaa
     4021 ttttgtagag aatcgagtga tatttctgca gtttttatat caaaaatatt ttctggaaac
     4081 gtgagcaaaa aacgcccttt gaaatcgacc aatgtagaat gtccgttata ttctataccc
     4141 tgtccatcgg ttccaattcg gtttaaacca cacacatacg cttgattttc aatagctcta
     4201 gcttgcaaca gaatatccca atcgcgaatt cgcttggaag gaaaattggc agtataaatc
     4261 agcaggtcgt aacgattgtc cacatttctc gaccaaacag gaaaacgtat atcgtagcaa
     4321 accaacacac agatattcca ttttttataa tgaacaagca ggtgtcggtt gccggcagaa
     4381 aaatattggt gttctccacc atgtcgaaac aaatgccgtt tatcggcagt ttgaatgttg
     4441 ccatcgggaa aaacaaaaaa actccggttg aaataacgat tattttcgca ggctataaaa
     4501 cttcctgtta gggcaatacc aaaattctta gcccaatttg aaagtgtttg aaccgtttcc
     4561 cccttccctt cttcagccaa atcgaggcga ttggtacaaa atccggtcga aaacatttcg
     4621 ggcaatacca ccaaatcggt ttccccttgc aaggtttcca aatgtcggtt tattctgtta
     4681 aaattggctt gtttgtctga ccaagctata acatcttgaa taagcgtgat tttcatattt
     4741 ttataaattt taaaaatcaa tccgattcaa ttttctaaaa aatagtcgga ttatactttt
     4801 cgggatatcg gggagaaata ttttgataaa actgctgaat ttcagaaaag tcagatgaaa
     4861 aatttcccga tggataaagc aatttcccta tcccgatttg tttttttttg taatcaatga
     4921 aggccaattc aataggcaca ttggcaccgg aagcaatata ataaaaacct tttttccaag
     4981 ctggcacgta acttctggtt ccttcaggcg ctataaccaa atgaaaacga tcatgttttt
     5041 caaactctgc tatcatttgc tcaactgtgg cattgctttt ggagcgatcg atcggaatac
     5101 ctccgacagc cttaaaaaaa taacccaacg gaaacacaaa ccatgatttc ttcaccaaaa
     5161 acgaaaattg catcccaact gaccacgaga aaagtttgcc gataacgaaa tcacaattgc
     5221 tggtgtgtgg agcaaaacag attacgcttt tttcaggaat ttcatgggga acaattattt
     5281 cccaactcac gcttttaaaa aacaaataaa ttaacttttt catcatttta gtctcgagga
     5341 aaattttaaa aagcaaataa aaacaatatt tcagttgacc agattcagtt caatgttgtt
     5401 tttatttccc aaaacagtaa attatttttc tatgtttaag tttcttccga aaaagttttt
     5461 caaagttaat tatttttaat agagatggag atcaaaaagc ataaaatttt tatgaaaccc
     5521 acatgtattg catacacaaa cacgaatgaa tataatattc agacatgtgg gtttcatcaa
     5581 acccttaaaa aataaattat attctgatga aatgaaaaaa gtgatgtaca attagatttt
     5641 tttctccggg aaaacaaata cgttgtgaac tcttgaaagt tgccactctt ttttaaattt
     5701 tcctgaattt tcggattttt ctgaaattat taccgatatt ttaatcattg aaaagacttc
     5761 cttttgtcaa aaagtttcta ttttctatta aaaggatatt atttttaatt cttgagataa
     5821 attttgccga caatttttca gaattcgagt ttttctattt atttttgttg tctaaatgat
     5881 acaataaaat tttatagcca tggaaaaaat tgatgagtta gacaggcaaa ttttaaagat
     5941 aattacgcaa aatgcgcggt tgccctttcg cgatgtagct gttcaatgtg gagtttcacg
     6001 agcagccata catcagcgcg tacaaaggct gatagatatg aatgtcatta ccggttcggg
     6061 atattttgtc aatcccaaaa aactcggtta tcaaatgtgt gtttatatcg ggattactct
     6121 tgaacgcggc tccatgtata aagaagtggt tcaggaactg gaaaaaattc ctgaaattgt
     6181 cgaatcgcag tacacgttag ggccgtacac cattttggta aaactgtatg caaagaatga
     6241 tgaacattta atggaattgc tcaacggaaa aattcaggaa ataaagggag ttgcaactac
     6301 cgaaacgctc acttcactcg atcaacggat tagacgtaca attcctatag attgaacgat
     6361 cgggggaaac tgctttaaag tcaatcaacc tatttttatt tttgcagcat gtttgctttt
     6421 aaggtgattc atgctgcttt ttattttttt gccgacatga aagttttggt aacgggagca
     6481 accggtttgt tgggacatca ggtaactatg gaattgattc agcatggtta tcaagtgcaa
     6541 attattgtcc gaaaaatgga taatattttt ttcgatctga atgcagttaa ggtttttatc
     6601 gggaatttta cagaatacga gcaattaaaa gctgcggctt cggggtgtga agccgtgata
     6661 catatcgcag cagttaccga tgtgcattta ctgcattatg aagattatcg gaaaataaat
     6721 gtcgaaggtt cggcacaggt aattcgtgtt tgcagagatt tgaatattcg caatgtggtg
     6781 tatatcagtt cttccaatac catcggtttt ggttcaaaaa gcaaacctgc cactgaagat
     6841 tttccttttc aatatccttt tacccgctct ttttacgcca gaagtaaagt tgaagctgaa
     6901 aagctgttcc agcaatttgc ttcggaattt ggtaatcatg taatcattat tcatcccaca
     6961 tttatgattg gagcttatga tgtaaagccg agttcaggga aattgctgct gatgggttat
     7021 cgaaagctac tcttgtttat tccgaaggga gggaaaaatt ttgttccggt tcgcgatgtg
     7081 gctaaggctg tatgcaatgc tctcacattg ggtaaaaatg gggaacatta tttgactgcc
     7141 ggcgttaatc tttcattcgc agaatattat cgtttgcaga aaaatatagc cggttaccgc
     7201 cagatcataa tggaagtccc ggattttttg ttgaaagtgt tgggaaaagt cggagatttt
     7261 cttcgttttt taggtatcaa aacggatgtt tgttccatga atctttctca gttgatgatt
     7321 cgggagtatt attgcggaca aaaagccata gacgagctca acatgcctca gaccgatttg
     7381 aaatttgcta ttgctgaagc tgtcgaatgg ttcaaagaaa gaggttggat acgctgaaaa
     7441 gagagaattc tcaatttttt ttgtgggcga aaaagttttt ttgtcagttc tgaaaaactt
     7501 caaccacttt tcccagttct gcataacaaa cgtaaccttt tgtttgataa cccatttgag
     7561 caattaaatc catgtattct ttcacttgtg caagatggta tttactttct ttctgaccga
     7621 atttgtaatc aatcacagta gctttagctt cttgaatgat cactcgatcc ggacgatgca
     7681 gacgattttc gggtgaaaga atggttgttt cgttcagtat tctgccttca ccgttgaacc
     7741 attcttttgc ttgtggaagc gaccagaatt tttccaatcc cttttcaacc gttatttttt
     7801 ctgcttcgct gatttgaccg gaagatacaa attcgagcaa cactttatcc tgatctgctt
     7861 ggttggtgat tttccgtaga atttcgtgca tgatgttgcc gtagttcagc tgactgtcgg
     7921 acaaatcttt ttcttcgatg agatattgag ttccttgatg ccgaatttgg agcttttgcg
     7981 cactgttgca cgaaggatat tgaacaaact tttcagattt tgtggatgaa attatttcct
     8041 tttgaggtgc atacgttgct tctccccatt cgtaaatttt attttcatca tcctttcgca
     8101 tcgacgaatt ttctccaata gtgctttgtt gtgtttgaac aaattgccaa agtaaggaag
     8161 aaacattttt tacagtttca gacgtagatt ttttcttttt ttcttcattt ttttcatcgg
     8221 gtgccggcgc catgcaaaca agttcgtttt ttggacgagt gaatgcaacg taagcaagat
     8281 tcagattatc gatatataag tgcatttgct catcgaaata attttgagta aaaatgctct
     8341 gtctcaaata ttgattgtag gaaatgggca gcagcggcaa ttcactgaaa ggcggaacgt
     8401 ttgtttcgca ccacagcaca ttggttttgt gtatattgcc ttccaatgcc caatcgcata
     8461 aaggaagaat aacgaccgga aaatcgagcc cttttgcctt gtgaatggtc ataatgtgga
     8521 tagcttcctg atcggccggc atggctacaa atttattgca gcccgcttcg tcccaccaag
     8581 atagaaaatc gctaatatcg gaaaaacctt tggctgtaaa attatatacc aaatcctgaa
     8641 aagcttgaac aaatggcgcc tgattttccc attcacctat attgaaaagg gaaataattt
     8701 gttctacgcg gtcaaaaagc gaataatgac gaattgcctg tagtgctttt tcttcttcgc
     8761 aatcaaatag atgggagaat ttattttcgg tattttcctt taatgcagca atgtcccgta
     8821 aggcttcatt ctttgttttt cccaatttat tcaacgcata ttcgaggctt acgattgtca
     8881 gttgaatgtc gtcggaagga gatacaaaaa gtttaagcag tccgacgata aaacgtaccg
     8941 aagatgcttt ccctatgagc aatccttcat ttcccaatat atcgtaacaa taaccttctt
     9001 tggctgaggg cgagtttttg tagttcatta aactttgaat gacaagggct gcgtcttttt
     9061 tttcgcgaac caatatggca atatcggaag ctttgtaacc acgatcctga aaattttcga
     9121 gcatgattgg caagcggttc aaactttctt cttgccagtt tttttcgtcg ttgggaataa
     9181 attggatgca cacatgccca gctccggctt tttctgaaac tttctgttcg gtgtctgtat
     9241 aggcatgttg aattttgttg gatagttcgg acacaaatgg atatttgtcg gccggcaaat
     9301 attcctgaat gttggcattc agctgttgtt gcagaagctg agcagattga gaaaaaaaat
     9361 tattgttaaa tttcacgata ttcctgtcgc tgcgccaatt gacgttgagt ggttcctggc
     9421 gaatttgttg cggagtaaaa tccttgaaaa tttgttcgtc caacaatttc caatcggaat
     9481 ttctccagcg gtaaatactt tgcttcacgt ctcccacaac catgttgaaa ttattttctg
     9541 ccaaactgtt tcgcacaagc gggagaaaat ttttccactg aagtaccgat gtgtcctgaa
     9601 attcgtcaat cataaaatgc tgcagcttgt tgccgatttt ttcgtaaacg taaggcgtat
     9661 cgctattgtc gataatacga ttgagcaaaa gattggtgtc ggaaattaaa aatgtgtttt
     9721 gttcggcagt caactgattg atttccacag ccaaatcaga aagaataccc agtttgtaaa
     9781 tgtgcttcaa tactatgtcg acggaattgt agcggataaa gggctcttga aaaatctcgt
     9841 taattttttc aaaacatttt cccaatccgc ttatataggc tgagttgact ttatttttta
     9901 cccaattggg tgtgctttta ttggtacagt tctctacatc atctgcatat tttctgaaac
     9961 ggctgttggg agcagctaca tttccactga tcagcttatc gaataatttt gtttccttat
    10021 aactaaaatc atcatgtgac agttcaaaat cggtcattat acgaatgccg tcctgtgcca
    10081 atttttttac ttcctcttca aagtctgttt tgattttttt cagtttgccg atatattttc
    10141 ctagaaaatc aagattgtga agttttttgc caatttcctc cgctctgtat tgatagtttt
    10201 ccttgaaaat ttcagcaccg aagttaataa tatcgttttt aagattccat ttcccgtttt
    10261 gttccatgct ttcttcggca aagctgatca gccaatccaa taacagtttg ttttcatttt
    10321 ttgataggtt gaaaaacaga ttgtcaacag cttgcgaaag caccgnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnttttc attttttgat aggttgaaaa acagattgtc
    10501 aacagcttgc gaaagcaccg cgtcgttttc catttcgagg ttgtaggctc cattgatacc
    10561 catctcacgt gcaaaactgc gaataacctg ctgaaagaaa ctgtcaattg tgctgacagc
    10621 aaatgaagag taatcgtaca acagacttac gagtatttgt ttggctttga gattgacctc
    10681 tgcttcattg cgagcgtatt cttccattaa actttttcgg taattgctgg gttgcccttg
    10741 tgccagcgca aaaagttcct tcagaatacg tgatttcatt tcttcggtag ctttgttggt
    10801 aaaggttacc gcaagaatat ggcgatgaag attttcacct tgatggcgga aaagcaaatg
    10861 aatgtaatct tttgtgagtt ggaaagtttt tcccgaccca gcagaagccc gataaacatt
    10921 caacataaaa aattttttta cagggtgatg aaaataaacg atttttactt gaaattgatt
    10981 tttttgactt tgtaacccat tttttggagt atttctgcaa ttttcacacg gaaatcgcct
    11041 tgtatgagta ccattccgtg ttctgccgtg cctcctgcgc tgcatgaaac ttttagcgtt
    11101 tttgcaagct ccttcagttc cttttcgttt ccctcaaatt cggaaacaat agtaaccggc
    11161 tttccgttgc gtttttccat ttgtactcgt aaaggttcgg tttgcacttt tttctccggc
    11221 agttcatttt ctgtcggttc actattatct tgtggcaaac tgtctttcag ttgattgagt
    11281 ttttctttcc agtccatgag gtaactataa ataagcggca agttaataat ttttttgtag
    11341 aaagagcaat agaaaaatga aatgaagaaa atattctttc agcttttcgc acatcttctt
    11401 caaattcaag gtagaagcca acaaaaaagc attcatttaa tgctgtcttt ttataggaaa
    11461 aaattctatt tcattcggaa atcttttgac gcatgtccga tgaattttag aaagcctgct
    11521 cttgcgaggt actccatcat catgcgtatc ggtacactcg aagctctgtt atccgaataa
    11581 tagggctcca aatttttatc aaaataactg cgattaattt ttttaatccg cagattcttt
    11641 tcgtagttta tgtcagtaaa gtttttctac cggggaccaa ataaattccg ttattttttg
    11701 caatttttct gtagtttttt tttggcattt tatagcttaa cttcactttg ggaaccaaaa
    11761 cttgcaggta tcccattttt ttttaatatc tttgcatatt gaaaatactc tcactataaa
    11821 gggaaaaaat gaaagaagtt acaaaagtat tgtatatttc ccaggaaatt tttccttatc
    11881 tgcctgcatc ggaaatggca aatatgggaa gatatcttcc tcaggcagta caggaaaaag
    11941 gattggaagt acgaattttc atgcccaaat tcggatctat taatgaacga agaaatcaac
    12001 ttcacgaagt aatcagactt tcaggaatga atatcattat tgatgatacc gaccatccgc
    12061 tgatcatcaa ggtagcctct attcctgctg cccgaatgca ggtatatttc atcgacaatg
    12121 aagattattt tcagcggaaa aatacggtgg ccaatgaaaa taacgaagag tatgatgata
    12181 atgacgagcg ggctatattt tttgcgcgcg gcgtgatgga aacggtaaaa aaactgcgct
    12241 ggtcgcccga tgtaatccat tgtcagggtt ggataacggc tttggtgcct ttgtatgtaa
    12301 aaaaaattta taacgacgat ccgtttttca agcattcaag agtggtttat tccattttcg
    12361 atgacgaatt taaaaagccc cttaacgatc gcttaatgga caagatttta atggatggca
    12421 ttcagaagaa acatttggct tccataaaaa atccacctgt cgattaccat tcacttctta
    12481 aacttgctgt cgattattcc gatggcttta tccaatccag tcccaaagtc aatgaatcgg
    12541 tgttgaagta tgttgccgac aaaaagatta agtttttgcc ttatcagccg gccgaagatt
    12601 attccgatca atatattgat ttttacaatc agcttttcta aagtttacat atcgataatc
    12661 atatattcaa tatgcatttc aacaaatttt tcctgtttct ttttacactg gcttttgttt
    12721 cgtgtacgga tggattgacc gacataggcg tcagcataat gccttcacag gatattatca
    12781 gtgttaacgc cgatgcattt gatttgaaca ccgaaaatgt ttttgttgat tttatttaca
    12841 cgcgtcagga ttctttcttg ttgggaaatt tttacgacca taaatacggt tctacccgcg
    12901 cagatattct ggcacaagta aactgccctg tcgattttac ttttcccgat aatgctgttc
    12961 ccgattcggc tgtaatagct ttgcgttatc tcacctggtt tggagataaa tattctccaa
    13021 tggaaatcaa aatttatcaa atgaataagg ctactttttc ctattcggag cattatccct
    13081 ccaatatcaa tccggatgat tataccgatc ggtctattct tttgggaaaa agaattatca
    13141 cgccaagcga tacttccggt tcgcgttccg atgggacaca agtacgtttc aagctttccg
    13201 atagttttgt tcaatggttt ttccccgaaa atcggaccta caacagtcaa aaggattttc
    13261 ttcagaagtt caaagggatg tttattacca caacttttgg cagcgcaaac attctttatg
    13321 tgaatcaaat cgatttgtac tattactatc attatactta tgtacgaaag acttccgatg
    13381 gttccgactc gacggttaca gtcaataatg ttattacttt tcctgccaat gccgaagttc
    13441 gtcaggttaa ccgtattcag catcctgaca ggaatgaagt cgttcaatcg cgtccagagg
    13501 tgaattatgt tgcctctccg gcaaatatca acacacgaat ttcgttgcct gtcgataaga
    13561 tagcgaagca aatgaaaaca accatccaca acaaatatct ttccgtgaac agtgcgttgt
    13621 tgaaagtaga ggttaccgaa caggatgaag caactattgc cgttcccctg gtgcaaaaca
    13681 tgatgttgat aaaagaatcg gctttggaca gatttttcaa aaacagcgaa ttgccttccg
    13741 atacctgtgc attgatagct cagtattcct atacaaaaaa tgccgatacc aatagttacg
    13801 attattatta cactttcaac ctttccaaat tgattgcgaa tgaaataaaa aaggctgaaa
    13861 ccaatggtac ccaacttccc gaaactatgg acttgttgct ggttcccgtt accgtgcaat
    13921 ataacaacag tgggtactac accagtgtga aacatcagat tttactgagt ggagtaacga
    13981 tcaaaagcgg gaaaaatact tcttccccaa tgaaaatcaa tttggtgtac agtggttttt
    14041 aaatcactgt aaattttatt tttttctgaa aaacagctga atgggtgttc cggtaaaatc
    14101 ccactgcgat ctgattttat tttccagaaa tcgccgataa ggttccttga tgtattgagg
    14161 caaattggca aaaaagataa atgtgggaac ttgcgtatca ggcagttgcg taacatattt
    14221 tatcttgatg tatttccctt tcagtgccgg cggaggaaaa ttctgaatca aaggaagcaa
    14281 atactcgttc aacttggcag ttggaatttt tttgtttttg ttttcgtaaa cttttactgc
    14341 cgattcgagc accttgaaaa ttctctgacg ggtgagtgcc gaaataaaaa taatcgggaa
    14401 atcggtaaaa ggagacatcc tttcccgaat atacgcttcc atttttttag tggaatgggt
    14461 gtctttgtct tctatcaaat cccatttgtt tacacaaacc actattccct ttttgttttt
    14521 ctgaataatg gagaagatgt tgagatcctg actttccaaa cctctggttg catcaatcag
    14581 cagcatacag acgtcgctgt tttcaatagc ccggatggaa cgcacgatgg aatagtattc
    14641 caaatcttca tctactttgg cttttttgcg aatgccggcc gtatcgacca gataaaaatc
    14701 gaatccgaat ttattgaaac gggtataaat agaatcccgc gtagtgcccg gaatttcggt
    14761 tacaatattt ctttcctcgt ccaaaaaaga gttgagaagt gaggattttc ctgcattggg
    14821 tcttcctaca atggcaaatc ttggcagatg ctcttccgtt tcttcatcct cttggggttt
    14881 caaataactt accagtttgt cgagcaaatc tccggttcca agcccgttga tggaagaaac
    14941 caaaaacgga tcacccatgc ccagtttgta aaattccggt gcctgatact gccattcgaa
    15001 tgtatcggct ttattggtaa ccagaataac cggtttgttg gtttttttga gaagttgcgc
    15061 aacttcaaag tccaagtcgg taatgccgca ctgtacatca accaaaaaaa gaattacgtc
    15121 ggcttcgttg atggccagtt gaacctgacg tttgatttcc tgttcaaagg tatcggaaga
    15181 gttggttacc caaccgccgg tgtcgatcac cgaaaattcg tatcccgacc attcgctttt
    15241 gccgtactgg cgatcgcgcg tagttccggc tgtttcgttc acaatggctc tccgcgtttg
    15301 cgtaagccgg ttaaaaagag ttgattttcc aacatttgga cgccctacaa tggctacaat
    15361 atttcccata attttttatt tttcatttgt aaatcttcaa tcggagtatt cccatattcc
    15421 gattgtgggt gaaaatattt tttaatttcg atgcaaagtt aagtaaaaca attgactttg
    15481 ctgtggtcgt gtttttttgg gctcagaaaa gcagggtact gaagccaatt tttttaattg
    15541 ccaaaatagc ggcgtaaaag aatattcaac ccctttgtct gttaattgaa ctgcttcatt
    15601 tgtgaaacca cctcgttgtg gatcaattcc gcaaattctt ccacgctcat gctccctgca
    15661 tcgccttctc cctgtcggcg taccgaaacg tgtgaagttt ccatttcctt ttctcccaca
    15721 ataagcatgt aaggaatacg tttcagttcg ttgtcccgaa tttttcgacc aattttttcg
    15781 tttctttcat ctacggtaac ccgaatggat ttttctttca gttttttggc aatatcgaaa
    15841 gcataatcgt tgaatttttc actgatgggc agcactacta cctgatcggg ggtaagccac
    15901 agcggaaatt ttcctgccgt atgttcaatc aaaacggcaa taaatcgctc catcgaacca
    15961 aaaggtgctc ggtgaatcat caccggcctg tgtttctggt tgtcttcacc ggtatattcc
    16021 agctcaaagc gttccggcag gttatagtcc acctgaatgg tgccaagctg ccattttctg
    16081 ccaatggcat cttttaccat aaagtccagc ttggggccgt aaaaagcggc ttctccctga
    16141 acaattttgg ctttcagccc tttttcttca caggcttcaa taatggcttt ttcggctttt
    16201 tcccaatttt cgtccgtgcc gatatatttt tccttattgt ccggatcgcg cagggaaatc
    16261 tgcgcttcaa aatttttaaa atcgagtgca ttgaaaataa tgaaaatgat gtccatcact
    16321 ttcaaaaatt catctttcac ctgatcggga cggcagaaaa tatgggcatc atcctgcgta
    16381 aaactcctga cgcgtgtcaa tccgtgaagt tcgccgcttt gttcgtagcg gtaaaccgta
    16441 ccaaattctg caaaacgcag tggcaaatcc ttgtaggaac gcggtttaaa tttgtaaatt
    16501 tcgcaatgat gcgggcagtt catcggtttg agcaaatatt cttctccttc ttcgggagta
    16561 tgaatgggct gaaaagagtc ttttccgtat ttggcataat gaccggaagt tacataaagt
    16621 tgcttgttgc caatgtgagg agtgatgact tgttgatatt caaatcgctg ttgaattgtt
    16681 ttgagtacat tttcaagcct gaggcgtaaa tctgtaccgc gaggcaacca aatgggaagc
    16741 cctttgccaa ccatttcgga aaacatgaac aattcgagtt ccttgccaat tttccgatgg
    16801 tcgcgttttt tggcttcttc caacaacaga aggtattcat ccagcatttt tttcttggga
    16861 aacgtaatcc cataaattcg agtgagttgc ttgcgctttt cgtcgccgcg ccagtaagcg
    16921 ccggccacac tcaacagttt gatggctttg attttttccg tattgggcaa atgaggcccc
    16981 cgacacaaat cggtaaaatt tccctgactg taaagagtta tggtgccgtc ctgcaaatcg
    17041 ttgattaatt ctactttgta ttcgtcgcct ttggtttgaa attttttcag tgcatcggct
    17101 ttgctgatgt ccgttctttt cagtttttcg ccgcgtgcag ccaattcgac catttttgct
    17161 tcaattgcgg gtaaatcgct ttcgcgaatg accgcttctc ccgggtcaac gtcgtagtag
    17221 aatccgtttt caatggccgg accaatgccg aattttattc cgggatacaa ttcctgcaat
    17281 gcttccgcca ttaagtgagc cgaagtgtgc caaaatgcat gtttcccttc ttcatcttcc
    17341 caagtattga tttttacggc agcatccgtt tcgatggggc gcgtcaagtc ccacacctga
    17401 ccgtttacgg ttatcgacaa ggcatcctga gccaatctgg agctgatgct ttcagctatt
    17461 tcaagtccgg taattccttt gggaaattcc tttaccgaac catcaggaaa tgttattttt
    17521 atcatgttca tgaaccttac aaatggtttt ttaatttttt cgcaaagata atggtttttc
    17581 ttcaaagccc gatgacttat tcggaaagag gaggcttcgg atgcattaga aaataaaaaa
    17641 atcaacccgc ttgcaggttt tttgggcatt ttgctccatt ggttttggtg tgctttgttt
    17701 tttccgaatg tcggatccgg acttgtttgg tttttgggaa aatacaatat ttttaccttc
    17761 ccccatgttt ggtgcggctt tgaattttca tcagaatata atttgttttt taaaggtctg
    17821 atggaaccca catgtctgaa tattctattc attcgtggtt gtgtatgcaa tacatgtggg
    17881 tttcttttgt ttttgccaaa actttttaat cgttcggaag atgaaaaatt gtcgtataat
    17941 ttttttatta tcctgttcta atttcaaaca gaaaaaattt gtttatatca attgaaattg
    18001 agacttaatt gattgctatc atctttatcg ataccgtatt ttaggggacg gtcagaataa
    18061 aattgatttt gaaatcttct aatatcgcct ttttcacatt tgttctttgg aaaaaattgg
    18121 atcctgatgc ggtttttatg ccgatatgaa ctgtttggat tgtctgggtt tttagtcatc
    18181 cgttggcaaa gaggtagaag aagatctaat aattcatttt tagaacgaga tttcttgctt
    18241 tcaggatttt caatgccatg tatcaaatta acgatagctc ctaaaaagca atcgcataac
    18301 tgtaaaatat ttgctttttc atcaatttta tgatctttac ctaaaaattg aatcttgtct
    18361 gtttcaaact taaaattatt ttttttgttt tgaagtttgt ctattggatg ccaagaaaaa
    18421 taagcgtgat cttgttgctg accttgttca tgaaatatat tttttataac tatttcatta
    18481 ttaccccaaa aacactttaa actatactcg attgcagttc taaaaaaccg attataaata
    18541 gaattgaact tattatcggc atcaaattca tcatcactta aataactttc attcagacct
    18601 aaaatataac aataaaactt atcgctactt ttttcaggat ttaaaatata ttgaaaccat
    18661 cgtttacaga tattgatttc atcggcgttt ttcatttcag tccaatgaag aattttgttg
    18721 tttttaaaat aatagggaga agctgaatca aaattattac aatacctttc gataattaaa
    18781 tcatccaaaa gaggcttgtc aaccctttcg aatattaaac acatataatg ccaattatgc
    18841 tttgtatcgg agcattttct gttcattgtt tcatcggcgt aaatattaac ctcaatttta
    18901 ggtggagccg gaatgacaaa gccaaataaa tctttttcag acatcgtata gttcctccac
    18961 ttttttgtct aattctattt gaattatgtc taattgtttt tcggcttcat cttc
//
LOCUS       DAOTAX010000039        18758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2442_length_18758_cov_30.690477, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000039 DAOTAX010000000
VERSION     DAOTAX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18758
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_18758_cov_30.690477"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLM04_05465"
     CDS             complement(<1..587)
                     /locus_tag="PLM04_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HON02558.1"
                     /translation="MNNFITNSNKKQLKTRLTELLSKSEELKFLVGFFYFSGLRELYD
                     GLQSNDKVKLKVLIGLNVDKTIYGLTEYGEKDKNLSDDERIENFFTSIRKSINSDVFD
                     NKDFYEQVKFFLQKIKDDEIILRKSREPNHAKLYIFKLEEGQIGRNKLFITGSSNLTS
                     AGLMSQAEFNVEISDYGFDEAESYFDELWNNAIKIT"
     gene            1368..4847
                     /gene="ileS"
                     /locus_tag="PLM04_05470"
     CDS             1368..4847
                     /gene="ileS"
                     /locus_tag="PLM04_05470"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HON02559.1"
                     /translation="MKKFAEYSSLDLSEVNKKMLLRWKEKDIFHKSIQIREGEPTFVF
                     YEGPPSANGMPGIHHVMSRTIKDLFCRYKTMQGFQVKRKAGWDTHGLPVELSVEKSLG
                     ITKEDIGKKISIEEYNAACRRDVMKYTREWEDLTTKMGYWIDLDDPYITYDNRYIETL
                     WWLLKQLYDKELLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTCVAQFKVKDT
                     SSLPFKTKINGDCYFLAWTTTPWTLPSNTALCVGPNITYVLVKTFNPYTGKPIKVILA
                     KDLVNTYFNQKNENLPFENYQPGDKNIPYRILDEFKGSELVGIRYEQIIPWVNPGENA
                     FRVIAGDFVSTEEGTGIVHIAPTFGADDDRVAKQNNIPPMLLLDKDGNKQPMVDRTGK
                     FFKLEDLDPEFVSKNVNISLYREYAGRYVKNEYISAFEPENQSDKNSSGEKTEDSTLD
                     IDLCVMLKQRGLVFKIEKYTHSYPHCWRTDKPVLYYPLDSWFIKTTALRDEMIALNQT
                     INWKPASTGYGRFGKWLENLQDWNLSRSRFWGTPLPIWHTEDGSEEICIGSVDELISE
                     IEKSIQAGFMTSNPYKNFKSGDYSRENYAVQNIDLHRPYVDNIVLVSKSGKPMRREPD
                     LIDVWFDSGSMPFAQLHYPFENREAIDQHTFYPADFISEGVDQTRGWFFTLHAISTMV
                     KGSVAYKNVVSNGLVLDKNGNKMSKRLGNVVDPFETIEKYGSDPLRWYMMTNSAPWDN
                     LKFDIEGVEEVRRKFFGTLYNTYSFFALYANVDEFTCSEPEIPVENRPEIDRWIISLL
                     NTLIKEVGEYYEDYDPTRAGRAISDFVTENLSNWYVRLNRKRFWGGEYNKDKISAYQT
                     LYTCLETVAKLMAPIAPFYADQLFLDLNEVTEKNNCESVHLSNFPVYDEKLIDKNLEE
                     CMQLAQQISSMILSLRRKAGKKVRQPLSKAIVPAADDKIYQQISYVADLIKAEVNIKE
                     LQVIPANMEMANLTKKVKLNFKVLGKKYGKQMKEIAEATAKLSNKEIAELEQNGIYIL
                     NLPGGEVQLTTDDVEIITEDMPGWLTANEGKLTVALDITVTDELLKEGIARELVNRIQ
                     NIRKQNGYEITDRITVEIEKRDEINDAVTEFSNYISSQTLAAGISLVDKLEDGIELDF
                     DEYMVKVKVEKTR"
     gene            4983..5360
                     /locus_tag="PLM04_05475"
     CDS             4983..5360
                     /locus_tag="PLM04_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HON02560.1"
                     /translation="MSEKTRYSDEELEEFRQIILEKLEKAQKDYELLRNGVTNSDGND
                     VTDTSPTFKVLEEGASTLSKEEAGRLAQRQLQFIQHLQAALVRIENKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKKSKK"
     gene            5444..6052
                     /locus_tag="PLM04_05480"
     CDS             5444..6052
                     /locus_tag="PLM04_05480"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HON02561.1"
                     /translation="MSTTKKSVLLIIFVLLVDQASKFYIKTHFALGDHIDIFSWFQIY
                     FIENNGMAFGIELLDKLFLTAFRIGAVVLIIFYIRSLIKRNFRTGYVLSISLLLAGAA
                     GNIFDSVFYGVLFSDSYGRVATFLPPEGGYAPLFYGKVVDMLYFPLIKNAAGETIFFR
                     PVFNVADAAITISVFIILIFYRKELNASFEKKEKTNVAEQSK"
     gene            6030..6887
                     /locus_tag="PLM04_05485"
     CDS             6030..6887
                     /locus_tag="PLM04_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HON02562.1"
                     /translation="MLLSSPNKIFSFFLLLALFLLPACDNRPKNVLSKKEMVKMLTEY
                     HLLHGTLQTTNQYYTPEGTLYFESLLKKHGITKAEFDSSMVWYAKNPKKFERIYAEVL
                     QNLNHLREDIVKGKFRSPDSILPGIEISNLWNLHPSYRFTPDSARTKLPFKIQQPYLM
                     TQDVYELQFLERIAPEDSCVDPYVVMKIHYANGKTDSIFQKTFNDSILRRYTLRLRAR
                     FPYKVDSVTGLLLGCKQYNGNFNVQLDSIRLLRYFVPQWQDSLKQVVEKNAAPPQKIQ
                     TKTIRPKEN"
     gene            complement(7061..7582)
                     /gene="ribH"
                     /locus_tag="PLM04_05490"
     CDS             complement(7061..7582)
                     /gene="ribH"
                     /locus_tag="PLM04_05490"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HON02563.1"
                     /translation="MSTEYKNLSSYNEDEMPDKSKVFLQQYGIVVADWNPQITHAMLE
                     GAYNALIENGSNPELINIVHVSGTFELTYGAKQLLEQYEEMDHIKRHKYAAIIALGCV
                     IQGETPHFNYVCEGVTNGIAYLNANSVGCPVIFGVLTTKTLQQALDRAGGINGNKGVE
                     AAITAIKMANIDW"
     gene            complement(7661..8338)
                     /locus_tag="PLM04_05495"
     CDS             complement(7661..8338)
                     /locus_tag="PLM04_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02564.1"
                     /translation="MSKKKGKGQDELANVEHALSASELFFEKYQKQILYVVGALIMVV
                     LVVLAVRNFYLTPREKAAQNEMYHAQYYFNLDSFQVALNGKEPEFMGFKQIVSEYGIT
                     PSGNLAAAYAGICYYKLGQYENAIKYLTQFDGNNSYFSTSVIGLLGDCFVEIGQNDKA
                     LTYFNKAIAVENEVLSPLYLKKAGLLCESMNQLKKAEEYFLQIKEKYPKSMEAGDIDK
                     YLARVQN"
     gene            complement(8817..10790)
                     /locus_tag="PLM04_05500"
     CDS             complement(8817..10790)
                     /locus_tag="PLM04_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HON02565.1"
                     /translation="MKNRYRSLFFAFFLALLPLSFTHAVKAYPFPVQITQPDGTVLTI
                     RIHGDEFHHIKTTVDGYLVKQNAKGYWTYASQDAAGRITPSEVIARNVSSRSAQDIQF
                     LKSLKISSSVSLQHVKASGRQFQKPQLSVRKNAFPLSNSPRSLVILVNFSDKSFVTSS
                     AQSAFTNLLNQSGYSANGATGSARDYFMASSYGKFSPEFDVVGPYTLSKTMQSYGAND
                     ADGYDVDPARMIVDACKAADNAGVNFSQYDTDNDGLIDNIFVYYAGYNEAEGASENTI
                     WPHRWVVYTSQESSTDYTYDGTVASVTFDGVRLYDYACTSELKGTSGSNMCGVGTFCH
                     EFGHVLGLPDYYHTAEDKTTLDYWNIMDAGNYLNDGRTPPVYSTYDRFYLGWLTPEEA
                     NTPSNLTLKPIYQGTTQPANTHQQSYLLSATTHNLVGNNPSPKEFFVLEYRKKTGWDA
                     YLPAEGMLIWHIDYDQTAWDNNEPNNYTGTTQTASSHMRVYLQPLSGSTTTPGTAFTS
                     GSFTPTTWSGTNINRAITEISKSSDQVTFKLMGGEIEDPNKAVIKLGVIQSQLVFPYT
                     AVNNAALKVINIKTNGITGNLTVTLSGDQAEMFTVSTNSITQNAANSTDGVNLNITYR
                     PTTSGEHTAVLTISGGGLSPDKVILLKGTGQPQ"
     gene            11585..13030
                     /locus_tag="PLM04_05505"
     CDS             11585..13030
                     /locus_tag="PLM04_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02566.1"
                     /translation="MLNIQKKMSNAFFALLSLPSTAMGFALSVQISALSWILSTKYGL
                     AIHEIGIVWAAGPIAGIIAQPIVGAISDKLWFMGGRRRPWIVLGGTMSALMLLALPNL
                     EVIQKIVVGGSDPQAGLLTIAIVVALTLDLSINVSFNPTRSLVADCTPLSERSKGFTW
                     MQTISGSFGVLAYLIGGTLGNIFLIYFGVGVAFLASVIPAFLIEEPRELKNFDAETQS
                     PKSKTTFRELWEAMLPLSGFLLYGIYVIFSKLGGFELKPTIPLFGYYLNVIEFLSILL
                     LLVSAIAVLLNLFKSGKKSNEFQKVLLAHSFTWIGVQTMFIYALFYLKDVLQLADNAG
                     SVNSIAFFVLSLVSAVLPVLVLNRLAEKYGMVKTHLSCIFIMALGYASILFFGHSVAV
                     YYFLIAIVGIGWGSVVSLPFAIMSEKVDQTKMGLYMGIFNLAVVLPQLVASFKMGEVI
                     NAAPNKSVIFAICAITLFISGILWLFVKNEK"
     gene            13200..13841
                     /locus_tag="PLM04_05510"
     CDS             13200..13841
                     /locus_tag="PLM04_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02567.1"
                     /translation="MFLNIPLLEWIGYLASIIVAISLTMSSIKKLRWLNLIGSAVFSF
                     YGFSIGALPVGFLNLFIVLVNIYYLIRMNSAQESFKALAVEPNDAYFNYFIEFHKKEI
                     LQIFPHFELASTKQDKTSSIALLLLRNAVAAGVFYGTIDNGCLYVELDFVASEYRDLK
                     PGEFIYKTNVKQLKETGIHKLVAKSEHPQHHKYLIKMGFVRRNDTPAVFEKEI"
     gene            complement(14021..16159)
                     /gene="pnp"
                     /locus_tag="PLM04_05515"
     CDS             complement(14021..16159)
                     /gene="pnp"
                     /locus_tag="PLM04_05515"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HON02568.1"
                     /translation="MTVVTKTITMSDGREIVIETGKLAKQADGSVMVRMGDTMLLATV
                     CSAQDANEGTDFMPLSVDYKEKFAAFGRFPGGFLRREGRPSDYEILISRLVDRALRPL
                     FPDDYHAETFVNIMLCSADGENIPDALAGLAASAALAVSDIPFNGPVSEVRVARIDGN
                     FVVNPTFNQLENADMDIIVAATMDNIMMVEGEMKEVSEDDLLDAMKVAHEAIKLHCKA
                     QLELMETVGKTKKREYCHEQNDEELKKDIWEKTYDKVYAEAASGNADKHSRSEHFEAI
                     MNEYIASLDTETAEAKKPLIERYYHAIEKEAMRRAILDEGKRLDGRKTTEIRPIWSEV
                     DYLPGTHGSSIFTRGETQALATVTLGTKMDEKLIDEPLIQGKERFLLHYNFPPFATGE
                     ARASRGIGRREIGHGNLAMRALKNVIPDDYPYVIRVVSDILESNGSSSMATVCAGTLA
                     LMDAGVQIKKPVAGIAMGLISENQGKNYAVLSDILGDEDHLGDMDFKVTGTREGITAT
                     QMDIKVDGLSYEILEKALRQAKEGRLHILNKMLETISEPRADFKPHVPRIVVMFVPKE
                     MIGAIIGPGGKIIQNIQLETGATITIEEIGDQGRVEIAATNKDSIEAAINKIKGIVAI
                     PEVGETYIGKVKSIMSYGAFVEFLPGKEGLLHISEIEWKRTESMDQTGIKEGDMIEVK
                     LLDIDEKTGKFKLSRKALLPRPPKEEKPRN"
     gene            complement(16416..17228)
                     /locus_tag="PLM04_05520"
     CDS             complement(16416..17228)
                     /locus_tag="PLM04_05520"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HON02569.1"
                     /translation="MLEKIQETADFLLAKMPMRPKTGIILGTGLGNLVTQMDIEMEIP
                     YETIPNFPVSTVEGHKGKLILGKLGGVEVLAMQGRFHFYEGYDMKAVTFPVRVMKALG
                     IKNLLVSNAAGGMHPDFEIGDLMIITDHINLFPEHPLRGKNFEELGPRFPDMSEAYCK
                     DFIVKAKQIAERNHIKVVEGVYVGTSGPTFETPAEYRYFRAIGGDAVGMSTVPEVIVA
                     NHAGMKCFGISIITDLGVPGKIVEVSHEEVQKIGDSVQPLMTLIMKELVKEI"
     gene            complement(17200..18330)
                     /gene="lpxK"
                     /locus_tag="PLM04_05525"
     CDS             complement(17200..18330)
                     /gene="lpxK"
                     /locus_tag="PLM04_05525"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HON02570.1"
                     /translation="MVSLLSSFSKNNPKLKKDLKHIVLYPLSLLYGLIVDIRNWLFDE
                     KIFHSVGFDVPTIVLGNLAVGGTGKTPHAEFVLSHLQHEFKTAFLSRGYKRKTKGFVL
                     ADENASAATIGDEPFQIFRKFPNVKVAVDEKRVEGIKRLLKLFPDLQLIVLDDAFQHR
                     YLLPGFSVLLTDYSNLYTSDFFLPAGSLRESKEGSHRANLIVVTKCPENLKPIDKRII
                     EMELHPLPYQQVFFSTFRYHDPVPVFPELTTENWTLSKLREESPAVLMVTGIASPQSM
                     KDFLKDYVSTLKEVSFPDHHKFSKNDFQLIRQEWDALPSENKIIIVTEKDAARLYNDK
                     YLPETLKAKMFALPIEVKFLFDQESLFIQKLKDYVRENSRNR"
     gene            complement(18455..18676)
                     /gene="yidD"
                     /locus_tag="PLM04_05530"
     CDS             complement(18455..18676)
                     /gene="yidD"
                     /locus_tag="PLM04_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HON02571.1"
                     /translation="MKKFFRWLVLLPVYFYRYSISPLFPPSCRYTPTCSQYTIDAVKK
                     YGVFKGGWLSVKRISRCHPWGGSGYDPVP"
BASE COUNT         5649 a         3578 c         3650 g         5881 t
ORIGIN      
        1 gtaattttta tggcattatt ccagagttca tcaaaataac tttctgcttc atcaaaacca
       61 taatcgctaa tttcgacatt aaattctgct tgcgacatta atccggctga agtcagattg
      121 ctgctgcccg taataaatag tttgttgcgc ccaatttgtc cttcttccag tttaaaaatg
      181 tagagcttgg catgattggg ttcgcgtgat tttctgagaa taatttcgtc atcctttatt
      241 ttttgaagga aaaatttcac ctgctcataa aaatctttat tgtcaaaaac atctgaattt
      301 atggactttc ggatggaagt aaaaaagttt tctattcgtt cgtcatcaga aaggtttttg
      361 tctttttcgc catattcggt cagtccgtaa atggttttgt ccacatttaa gccgatcagc
      421 acttttagtt tcactttatc gttcgattgc aagccatcgt acaattctct taaaccagaa
      481 aaataaaaaa atccgaccag aaatttcagc tcttcacttt tggatagaag ttcggtcaga
      541 cgtgttttta actgcttttt gttgctgttg gtaatgaaat tgttcataat tttttattga
      601 tcaataaaaa aattaaactg aaaaggaaaa ttctttttaa aatgattcgg caaaatatct
      661 tttaataaat tggcagctta ttgattaaaa agcaaagttt cacgcaaaaa aattgattaa
      721 gcagctgatg gtcagtggat gcatgctaat cccaaacact ttaaagatgc aacaaaccgc
      781 aaactcaatc aatataggag tttctgtagt aactattagt tatcccggct acgaagttaa
      841 ataaaagttt taaatcttac cttaatggag atattaattt tatctgccgt gatgtcttgg
      901 gtcgctgtat cttaatattt tggtcgggta cgggcaaaaa ttaagggatt tctttttcaa
      961 tataattttg taaaacaatg atatacaaag cctgctggta agccttcgga catttttccg
     1021 atatattttt aacaaactgt tgatgttgaa aaaccgcatc ggaatcgggc gatgtagttt
     1081 tcggagaaat aaatcaagga ggaaacacca aacaaggcaa cctttttttc ttcaaataat
     1141 tattactctt tcagttccat aaccaatgcc cgaatacttc tttgtattcg acttttcgtt
     1201 tttcaataaa atttagtaat tttgcacctt ccaaaaaaac agcaaagaaa gccgaaaaaa
     1261 gcaggaagga atctcagaaa ttgagttcta tcaaaaattt ttatgcaatt cgggttttca
     1321 gcgtttttgc tattggttaa agacaggaat cagaggataa acacgatatg aaaaagtttg
     1381 cagaatactc ttctttagat ctttcggaag tcaacaaaaa aatgctttta cgttggaaag
     1441 aaaaagacat tttccacaaa agcattcaaa ttcgagaagg agaaccaacg tttgtatttt
     1501 atgaaggccc gccttcggcc aacggtatgc cgggcatcca tcatgttatg tcccgaacca
     1561 taaaagacct tttctgccgt tataaaacca tgcagggttt tcaggttaaa agaaaagcag
     1621 gatgggatac gcatggactt ccggttgagt tgagcgtgga aaaatcattg ggaatcacta
     1681 aagaagatat tggtaagaaa atcagtatag aagaatacaa tgcagcttgt cgtcgggatg
     1741 taatgaaata tacccgcgag tgggaagatt tgaccacaaa aatgggttat tggattgatt
     1801 tggatgatcc ttacattacc tacgacaatc gttatattga aacactttgg tggttgctta
     1861 aacagcttta tgataaagaa ttgctttaca agggctatac catacaaccc tattcaccgg
     1921 ctgcaggaac cggattgagc acgcacgaac tgaatcagcc gggttgctat cgcgatgtaa
     1981 aagatactac ctgcgttgct cagttcaaag tgaaggatac ttcttctttg ccattcaaaa
     2041 ctaaaataaa tggcgattgc tattttctgg catggaccac tacaccttgg actttacctt
     2101 ccaatacagc cttgtgcgta gggccgaata ttacgtatgt gcttgtaaaa acgttcaatc
     2161 cttataccgg caaacccata aaagtaattt tggccaaaga tttggtaaac acttatttca
     2221 accaaaaaaa cgaaaatctt ccttttgaaa attatcagcc gggtgataaa aatatacctt
     2281 accgcatttt ggacgaattc aaaggcagcg aattggtcgg cattcgatac gaacagataa
     2341 ttccgtgggt taatcccgga gaaaatgctt ttcgtgttat tgcgggagat tttgtttcta
     2401 cggaagaagg aaccggaatc gttcacattg caccaacttt tggggctgat gacgataggg
     2461 tggcaaaaca aaataacatt ccgccgatgc tgttgcttga caaggacggg aacaagcaac
     2521 caatggtgga cagaacgggt aaatttttta aattggaaga ccttgatccc gaatttgttt
     2581 ctaaaaatgt aaatatttcg ctttacagag aatacgccgg tagatatgta aagaatgaat
     2641 atatttcagc ttttgaaccg gaaaatcagt cagataaaaa ttcgtctgga gaaaaaaccg
     2701 aagattcaac gctggatatt gacttgtgcg tcatgctcaa acaacgggga ttggtattca
     2761 aaattgagaa atacacacac agttatccgc attgctggcg aaccgacaaa ccggtgttat
     2821 attatccgct ggacagctgg tttattaaga ctaccgcttt acgggatgaa atgattgcat
     2881 tgaaccaaac catcaattgg aaacctgctt ctacaggata tggacgtttt ggaaaatggc
     2941 tcgaaaatct tcaggattgg aatctttcac gaagtagatt ttggggaact ccgcttccca
     3001 tttggcacac tgaagatggt tcggaagaaa tttgcattgg gtcagtggat gaattgattt
     3061 cagaaattga gaaatccata caagccggtt ttatgacatc caatccctat aaaaatttca
     3121 aatccggcga ttattcacgt gaaaattatg ctgttcaaaa tatcgatttg catcgtcctt
     3181 atgtggacaa tattgtgctc gtatctaaat cgggaaaacc catgagaaga gagccggact
     3241 tgattgacgt ttggttcgat tccggttcga tgccatttgc acagttgcat tatcctttcg
     3301 agaacagaga agccattgat caacatactt tttatcctgc cgattttatt tcggaagggg
     3361 tagatcagac acgtgggtgg ttttttactt tacatgccat cagcacaatg gtgaaaggtt
     3421 cggtagccta taaaaatgtt gtttccaatg gcttggtgct cgacaaaaat gggaataaaa
     3481 tgtcgaaacg tttgggcaac gtggtagatc cttttgaaac catcgaaaaa tacggttctg
     3541 accccttgcg gtggtatatg atgaccaatt ctgctccctg ggacaatctg aaatttgata
     3601 ttgaaggagt ggaagaagta cgaaggaaat tctttggaac gctttataat acttattctt
     3661 tctttgctct ttatgccaat gtagatgaat ttacttgcag cgagcccgaa attcctgtgg
     3721 aaaatcgtcc ggaaatcgac cgatggataa tatcattgtt aaatacattg ataaaggaag
     3781 ttggtgaata ttacgaagat tacgatccaa caagagcagg cagagcaatt tccgattttg
     3841 ttaccgaaaa tctgagcaat tggtatgttc gcttgaatcg gaaaagattt tggggtggag
     3901 aatacaacaa ggataaaatc tcggcttatc aaacgcttta tacctgtttg gaaaccgttg
     3961 ccaaattaat ggctccaatc gctccgtttt atgccgacca attgtttctc gacttgaatg
     4021 aagttacgga aaaaaataat tgcgaatctg ttcatttgag caattttccc gtttacgacg
     4081 aaaagttaat cgacaaaaat ctggaagaat gtatgcaact ggctcagcaa atctcctcca
     4141 tgattctttc tctgcgtcga aaagccggga agaaagttcg tcagccactt tctaaagcta
     4201 ttgttccggc tgccgatgat aaaatctatc agcaaattag ttatgtggcc gatttaatta
     4261 aagccgaagt aaacataaaa gaattgcaag tgataccggc caatatggaa atggcaaatt
     4321 taaccaaaaa ggtaaaactc aattttaagg ttttgggtaa aaaatacggc aaacaaatga
     4381 aagaaattgc agaggcaacc gcaaaattga gcaacaaaga aattgccgaa ctggagcaaa
     4441 atggaattta tattttaaat ttgcccggtg gggaagttca acttacaaca gatgacgtcg
     4501 aaataataac agaggatatg ccgggctggc ttactgccaa tgaagggaaa cttaccgttg
     4561 cgctggatat taccgtgacg gatgaattgc tcaaagaagg aattgcgcgc gaattggtaa
     4621 acagaattca aaatatacgc aagcaaaatg gctatgaaat aaccgacaga ataactgttg
     4681 aaattgaaaa aagagatgaa atcaacgatg ccgttacgga attttcgaac tatatttcct
     4741 ctcaaacatt ggctgccggc atttctttgg tagataaact ggaagatggc attgagcttg
     4801 attttgatga atatatggtg aaggttaaag ttgaaaaaac acgctgaaaa agccacaaaa
     4861 ttattactaa aataattgga aaagagaaaa aaaacgatta ttttcgcaca ttattaaaaa
     4921 ctcagcattt gcagaatgct gaaccgaaac aaagggatta aacaaaataa agttttacga
     4981 ttatgtcaga aaaaacaaga tattcggatg aagagctgga agaatttcgt cagattatcc
     5041 tcgaaaagtt ggaaaaagct caaaaagatt acgaattatt acgcaacggt gttaccaata
     5101 gtgatgggaa tgatgtaacc gatacttctc caacctttaa ggttttggaa gaaggtgcat
     5161 ctacactttc aaaagaagaa gccggacgtt tagctcagcg tcagctccaa ttcattcagc
     5221 atttgcaggc tgctcttgtt agaattgaga acaaaacata cggaatttgt cgcgagaccg
     5281 gcaagctgat ccctaaagaa cgcttgcgtg ccgttcctca tgctacacta agtattgaag
     5341 ccaaaaagtc gaaaaaataa tttatatatt ctatccacta ttaaagacgg caacagagta
     5401 tttttctttg ttgccaattt tttaataaaa gcagcttttg ttcatgtcaa ctacaaaaaa
     5461 atcggtttta ttgataatct ttgttttgct tgtagatcaa gccagcaaat tttacatcaa
     5521 aacgcatttt gctttaggcg atcatatcga tatttttagt tggtttcaga tttattttat
     5581 cgaaaacaac ggaatggctt tcggcattga attgttggac aagcttttcc tgactgcttt
     5641 cagaattggc gcagttgttt tgatcatatt ctacattcga tcgctaatta agcgaaattt
     5701 tcgtaccggt tatgtgttgt ccatttcatt gcttttggcc ggcgcagcag gaaatatttt
     5761 cgactctgtt ttctatggcg tccttttcag cgacagctat ggaagagtgg ctactttttt
     5821 acctcccgaa ggcggttatg ccccgctatt ttacggaaaa gtggtcgaca tgctttattt
     5881 cccgcttata aaaaatgctg caggtgagac aatatttttc cgacctgttt tcaatgttgc
     5941 cgatgctgcc attacaattt ccgtgtttat tattctgatt ttctatcgaa aagaattaaa
     6001 tgcaagtttt gagaaaaaag aaaaaaccaa tgttgctgag cagtccaaat aaaatctttt
     6061 ctttcttttt gctcctcgcg ctttttctgc tgccggcttg cgataatcga cccaaaaatg
     6121 tgttgagtaa gaaggaaatg gtgaagatgc ttaccgaata ccatttgttg cacggcactc
     6181 ttcagacaac aaaccaatat tatactcctg aaggtacttt gtattttgaa tccttactta
     6241 aaaaacacgg aattacaaaa gcagaatttg attcttctat ggtttggtat gccaaaaatc
     6301 ccaaaaaatt tgaacgcatt tatgctgaag tactgcaaaa tcttaatcat ctcagagaag
     6361 acatcgttaa ggggaagttt cgctcgcctg attctatttt acccggaata gaaatcagta
     6421 atttatggaa tcttcatccg agttatcggt ttactccgga ctcggctcga acgaaattgc
     6481 cttttaaaat tcagcagcct tatctgatga cacaagatgt ttacgaactt caatttttgg
     6541 aaagaattgc tcctgaagat tcctgtgttg acccttatgt agtaatgaaa attcattatg
     6601 cgaatgggaa aacggacagt atttttcaaa aaactttcaa cgacagcata ttgcgtcgtt
     6661 acacgctccg acttcgggcc agatttccct ataaagtcga ttctgttacc ggcttgctgt
     6721 tgggttgcaa acaatataat ggaaatttca atgttcagct tgacagcatt cggttgttac
     6781 gctattttgt tcctcagtgg caagatagtt tgaagcaggt tgttgaaaaa aatgcagctc
     6841 ctcctcaaaa aattcaaacc aaaacaattc ggccaaaaga aaattgaata attccaattt
     6901 tcactatccg ataaaaagta aaaagcgatc agtaaatggt cgcttttcat tagaggaaaa
     6961 aagaacaatc ctcaaacccc tgccctcaac ttggaaagcc aatgctctat tgaccaagct
     7021 gcttgcggaa aagttgtaaa atattggttg agaaaaatat ttaccaatcg atatttgcca
     7081 tttttatagc ggtaatggca gcctcaacgc ctttgttgcc attgattcct ccggcacggt
     7141 cgagagcctg ctggagtgtt ttggtagtaa gaacaccaaa aatcaccgga caacctactg
     7201 aattggcatt taaatatgca attccgtttg taacaccttc gcagacataa ttaaaatggg
     7261 gcgtttctcc ttgaattaca caacccaaag ctatgatagc tgcatatttg tgtcgtttta
     7321 tatggtccat ttcttcatat tgctccaaca gctgtttagc gccataagtc agctcaaaag
     7381 tacccgatac atgaacaata ttgattaatt cgggatttga gccgttttct atcaaggcat
     7441 tataagctcc ctccaacatg gcatgggtga tttgaggatt ccaatcagct accacaatgc
     7501 cgtattgttg cagaaaaacc ttgcttttat ccggcatttc atcttcatta taggaggaaa
     7561 gatttttata ttctgtggac attgggttat aatgtaattt tttatgatat ttttttaaaa
     7621 aaacaatttt ttcaaatttt acccgacaaa aaacgtcaaa ttagttctga acgcgtgcca
     7681 gatatttgtc gatgtcgcct gcctccatac tttttggata tttttcctta atttgaagaa
     7741 aatattcttc ggccttttta agttgattca tcgattcgca aagcagaccg gcttttttca
     7801 aataaagggg gctcaaaacc tcattttcaa ctgcaatagc cttgttgaaa taagtcaaag
     7861 ctttatcatt ctgaccgatt tctacaaaac aatcgcctaa taaaccaatg acagaagtag
     7921 agaaataact gttgtttcca tcaaattgag tcaggtattt gatggcattt tcatattgcc
     7981 ctaacttata atagcaaata ccggcatatg cagcggccaa attacccgaa ggtgttattc
     8041 cgtattccga tactatttgt ttaaaaccca taaactcggg ttctttccca ttaagtgcaa
     8101 cctgaaacga atcgagattg aaatagtatt gagcatggta catttcattt tgtgctgctt
     8161 tttcacgagg agtcaaataa aaattacgca ctgccaatac aaccagaaca accattatca
     8221 aagctcctac tacgtataga atctgcttct gatacttttc gaaaaacagt tcggatgctg
     8281 ataaagcatg ttccacattt gccaactcgt cttgaccttt tccctttttc ttcgacataa
     8341 gaatggttat ctttaggatt ttaaatttgt gtgttgttta aataatagaa tgcaaaatta
     8401 aagattttct ttcggataat gaaattttat ggtaagtttt tcacatagca gactcaacca
     8461 gccagtgcga aatttacata aaatgtttga taaattggaa ttttggtaaa tttcagttta
     8521 ttcatgcatc ataaaaaaat ccatgacttt tttacaaatt tgaatcagcc gatatacaat
     8581 acagtttgaa aaaccaaaca acaacgatat aaagatgcca gccaagaaac attaaatggt
     8641 tactaagatt acaagtttat ggataaggtt tttctttttt ctcctgtttt ccgaactttt
     8701 tcaagacacc catgttaccc tatagtgggc aatattttta aacacgaaaa aaaatcgatt
     8761 taaaaagtag gcgcttgctt tttaaatcga ttttcgtcaa tgttcattca accattttat
     8821 tgtggttgtc cggttccttt cagtagaata actttgtcgg gagaaagtcc gccacctgaa
     8881 atagtgagca cagcggtgtg ttcgcctgaa gtcgttggcc gataagtaat gttgagattt
     8941 acaccatcag tgctattggc tgcattttga gtaatggaat ttgtcgaaac agtgaacatt
     9001 tctgcctgat cgccgctgag ggtaacggtc aaattccctg ttattccgtt cgttttaatg
     9061 ttgattactt tcagggcagc attgtttacg gctgtgtaag gaaaaacaag ttgagactga
     9121 ataacgccga gttttatcac agctttgttt gggtcttcga tttcgccacc catcaacttg
     9181 aaagtaactt gatcagatga tttggaaatt tctgtgatag ctcgattgat gtttgttccc
     9241 gaccatgttg ttggtgtgaa tgatccgctt gtgaaagctg ttcccggagt ggtggtagag
     9301 ccgctcaaag gttgcaggta aactctcatg tggctgcttg ctgtttgagt agttccggtg
     9361 tagttgttgg gttcgttgtt gtcccaagcc gtttgatcgt aatcgatgtg ccaaataagc
     9421 attccttcgg caggtagata agcatcccaa ccggtctttt tacgatattc gagtacaaaa
     9481 aactcttttg gagagggatt atttcctacc aagttgtgtg tagtagccga aagtaaatag
     9541 ctttgctgat gggtgtttgc cggttgcgtt gtgccctgat aaataggttt cagagtcagg
     9601 ttgcttggtg tattggcttc ttcgggagtt agccagccca aataaaaacg gtcgtaagtc
     9661 gaataaacgg gaggcgttct tccatcgttc aagtaattac cggcatccat aatgttccag
     9721 tagtctaatg ttgttttgtc ttcggcagtg tgataataat cgggtaaccc cagaacatga
     9781 ccgaattcat ggcaaaaagt tcccactcca cacatatttg aaccggaagt tcctttcagt
     9841 tctgaagtac aggcgtaatc gtacagccga acgccatcga aagtaacaga agccactgta
     9901 ccatcataag tataatcggt tgaagattct tggctggtat aaactaccca tcggtgtggc
     9961 caaatcgtat tttctgatgc gccttccgcc tcgttgtagc cggcgtaata aacgaatatg
    10021 ttgtctatca gtccatcgtt gtcggtgtca tattgcgaaa aatttactcc ggcattgtcg
    10081 gctgccttac aggcatcaac aatcattcgt gccggatcca catcgtatcc atccgcatca
    10141 ttggctccgt aactttgcat tgtttttgat aaagtgtaag ggcctaccac atcaaattct
    10201 ggagaaaatt ttccgtaaga gcttgccatg aaatagtcgc gtgccgaacc agtagcacca
    10261 ttggcactgt aaccgctttg attgagcaga ttggtaaaag ccgattgagc agaagaggtt
    10321 acaaaacttt tatcggaaaa atttaccaga ataaccagcg aacgtggtga atttgacaga
    10381 ggaaaggcat ttttgcgaac agaaagttgt ggcttctgaa attggcgacc ggaagctttt
    10441 acatgttgca gggaaacaga ggaagaaatt ttcaaacttt tcagaaattg aatatcctgt
    10501 gcggaacgac ttgaaacatt tctggctata acttcacttg gagtgattct gccggcagca
    10561 tcctgtgaag catacgtcca gtaacctttt gcattttgtt ttactaaata gccatcaaca
    10621 gttgttttaa tatgatgaaa ctcatcacca tgaatgcgaa tggtaagcac agttccgtcc
    10681 ggttgcgtaa tctgaacagg aaatggatag gctttaacgg catgagtgaa actcagaggc
    10741 aacaaagcaa gaaaaaaagc aaagaaaaga gaacgataac gattcttcat gggtttaatt
    10801 tttttgttga acaataaaat ctgaaactat ctaacctgct tattataaac atactacaaa
    10861 attttgtttc catattttaa tttgtttgga aaatattagg gtcgcaaaga taacaataat
    10921 tttaaattta aaatggtttt ctgaaaaatt gttggttttt tcgagaaaat ttaaaatctt
    10981 catgttctaa aatagattga caaaaataca tcactaccga atatacaaaa aatttgcctg
    11041 acataaaatg atcagctcgg cattgatggt agatttcttt tttcaaaatt tttcattcgt
    11101 gggaaaggaa gtctctgaaa atactcacag acttcttcat cagtattctc ctgaaaaaac
    11161 ttcttttgtg aatatctctg atcaagatat aaagcgagta gaagatattt tgaacaacag
    11221 accaagagaa aattggattt catcagaata taatttattt tttaatggtc tgatggaacc
    11281 cacatgtctg atatattcag ttgttcgtgt ttgtgtattt aatagatgtg ggttgcttaa
    11341 catcaaatga atttttatta cgaaatttgt gttaccaaaa agttgtattt actaattgaa
    11401 tttacaactc taatttttgt ggttgataag aaattttgct tatccctaca tctctgcaaa
    11461 cgtttgtcct gaaattttat cgaattttca ttcaaatttt cctttcattc ataaaaaaat
    11521 aatatcattg cagcgtttta ttccatcaaa tttttcaaga ataaatttat aaaaaaactc
    11581 aaccatgtta aacattcaaa agaaaatgag caatgctttt tttgctcttc tcagtttgcc
    11641 ttctacagca atgggttttg ccctgtcggt gcagatttcg gcattaagtt ggattttatc
    11701 taccaaatac ggattggcta ttcatgaaat aggtattgtt tgggcagccg gaccgattgc
    11761 gggaattatt gcccagccaa ttgttggagc tatttccgat aaattgtggt ttatgggcgg
    11821 caggcggagg ccttggattg ttttgggtgg aacaatgtct gctttgatgt tgctggcttt
    11881 gccaaatctt gaagttatcc aaaagattgt tgtaggtgga agtgatccac aagccggact
    11941 tttgaccatt gccattgtgg tagcactgac gctcgatttg tccatcaatg tcagttttaa
    12001 tcccacccgt tcgctggtag ccgattgtac gcctttgtcc gaaagaagca agggatttac
    12061 ttggatgcaa actatttcag gttctttcgg cgtgttggca taccttattg gcggaacgct
    12121 gggtaatatt tttcttatat attttggtgt tggcgttgcc tttctggctt cggtcattcc
    12181 ggcttttctt atcgaagaac ctcgcgaatt gaaaaatttt gatgctgaaa ctcagtctcc
    12241 caaatcaaaa acaactttcc gtgaattgtg ggaggcaatg cttccgcttt ccggattttt
    12301 actttacggt atttacgtga ttttttcaaa attgggcgga tttgaattga aaccaaccat
    12361 ccctcttttt ggttattatt tgaatgttat cgaattttta tctattctgc ttttgctggt
    12421 aagtgccatt gctgttttgc tgaatctttt caaatccgga aagaaaagca acgagtttca
    12481 aaaagtcttg ctggcacatt cttttacatg gataggcgtg caaacgatgt ttatttatgc
    12541 tcttttttat ttgaaagatg ttttgcaatt ggcagataat gcaggttcgg tcaactccat
    12601 tgcgtttttt gttttaagtt tggtaagtgc cgttttaccg gttttggtat tgaatcggct
    12661 tgcggaaaaa tatggtatgg taaaaacaca tttgtcttgc atttttatca tggcattggg
    12721 atatgcatcc attttatttt tcggacacag tgttgccgta tattattttc tgatagccat
    12781 agtgggaatt ggctggggat cagtcgtttc gttgcctttt gccattatga gcgaaaaggt
    12841 ggatcaaacc aaaatgggac tttacatggg aatattcaat cttgccgtag tgttgcctca
    12901 attggtggca agtttcaaaa tgggtgaagt aatcaacgct gctcccaata agtccgtcat
    12961 ctttgccatt tgcgccataa cgctttttat ttcaggtata ttgtggttgt ttgtgaaaaa
    13021 tgaaaagtaa gtttttagca atttttcttt tttcaagagc aatggattta tgtttttttg
    13081 aattaatatt tgaaaagtaa acagaaaata tttacttttg cgctgaaagt aaatcgacgt
    13141 ctaaagatgt agatttactt ttttgtttct ttaatcatta tattttaaaa ccaataatca
    13201 tgtttctaaa cattccactc ttagaatgga ttggctatct ggcatccatc atagttgcta
    13261 tttctctgac aatgagttcc ataaaaaaac ttcgatggtt gaatctcata ggttctgccg
    13321 tgttttcctt ttatggattt tctattggag ctttaccggt tggattcctt aatttgttta
    13381 ttgtactggt aaatatttat tatctgatca ggatgaattc ggctcaagaa tctttcaaag
    13441 cacttgccgt tgaaccaaac gatgcttatt ttaattattt tatcgaattc cataaaaagg
    13501 aaattctaca gatttttcct catttcgagc ttgcttcaac gaaacaagat aaaacttctt
    13561 ccatagcttt acttttgttg cgtaacgccg ttgctgccgg agttttttat ggaacgatcg
    13621 acaatggttg tctttatgtt gagcttgatt ttgttgcttc agaatataga gatctgaaac
    13681 cgggagaatt tatctacaaa accaatgtga aacagctgaa ggaaaccggc attcataaac
    13741 ttgttgccaa atcggaacat ccgcagcacc ataaatattt gataaaaatg ggttttgttc
    13801 gtcgcaatga tacaccggct gttttcgaaa aggaaattta atcttgatta aaataatctc
    13861 cagataggtt caaaagtaag ctctttaata gaattttttt tcttttcaac ttggcacatg
    13921 caggaactct catcctatta gcttaatacc atgcccgaca tactaaaaaa aagaaagtgt
    13981 ccttttggga cactctctta tttgataatg tttaacttaa ttagttcctg ggcttttctt
    14041 cttttggtgg acgtggaaga agagctttcc gtgacagttt gaatttaccc gtcttttcat
    14101 caatatcgag aagtttcact tctatcatgt ccccttcctt gataccggtt tggtccatgc
    14161 tttcagtacg tttccattca atctccgaaa tatgcagcaa accttccttg ccgggtagaa
    14221 attctacaaa agcaccatag gacataatgg attttacttt tcctatataa gtttcaccaa
    14281 cttcaggaat agcaactatt cctttaattt tattgatggc agcttcaatt gagtccttat
    14341 tggtagcagc aatttccaca cgtccctgat cgccaatttc ttcaattgtg atggtagcac
    14401 ctgtttccag ctgaatgttt tggataattt ttccgcccgg gccaataatt gcgccaatca
    14461 tttcttttgg tacaaacatt accacaatgc gcggaacatg cggtttgaag tcggcacgcg
    14521 gttcagatat ggtttccaac attttgttca ggatatgcaa tcgtccttct tttgcctgtc
    14581 tcagagcttt ttccaaaatt tcgtacgaaa gtccatcaac tttaatatcc atttgtgtgg
    14641 cagtaatacc ttctctggtg ccggtaacct taaagtccat atcgcctaag tgatcttcat
    14701 cacccaaaat atcggaaagc acggcataat tttttccctg attttcagaa atcagtccca
    14761 tagcgatacc ggcaacaggt tttttgatct gcactcctgc atccatcagt gctaaagtac
    14821 cagcacaaac agttgccata gaagacgatc cgttggattc caaaatatcg gaaacaacgc
    14881 gaataacata aggataatcg tccgggataa cattttttaa ggctctcatg gccaggttgc
    14941 catgtccaat ttcccgacgg ccaatacccc gcgaagccct tgcttcacct gtggcaaaag
    15001 gtggaaaatt gtaatgaagc agaaaacgtt cttttccctg aattaaaggt tcatcaatca
    15061 acttttcgtc cattttggtt cccagtgtta cggtagccaa tgcctgagtt tcacctcgcg
    15121 taaaaatgga agaaccgtgc gtccccggca aataatctac ttccgaccat attggacgga
    15181 tttcggtagt tttacgtcca tccagacgct ttccttcatc aaggatagcc cgacgcatgg
    15241 cttctttttc tatggcatga taatatcttt cgatcagcgg tttttttgct tcggctgttt
    15301 cagtatccaa cgatgcaata tattcgttca taatggcctc aaaatgttcg ctgcgcgaat
    15361 gtttgtcggc atttccggaa gctgcttcgg cataaacctt atcgtaggtt ttttcccaaa
    15421 tgtccttttt cagctcttcg tcgttttgtt cgtggcaata ttcgcgtttt ttagtcttgc
    15481 ctactgtctc cattaattcc aactgagctt tgcagtgtag ttttatggct tcatgagcaa
    15541 ctttcatggc atcaagcaaa tcgtcttcgc taacttcttt catttcgccc tccaccatca
    15601 tgatattgtc catagtagca gccacaataa tgtccatatc ggcattttcc aactgattga
    15661 aagttggatt cactacgaaa tttccatcaa tgcgtgccac gcggacttcc gatacgggcc
    15721 cgttgaaagg aatgtcggaa acggcaagtg cagcagaagc tgccaaacct gcaagcgcat
    15781 ccggaatatt ttccccatca gccgaacaaa gcataatgtt cacgaaagtt tcggcatgat
    15841 aatcatctgg gaaaagtgga cgcaaagccc tgtcaaccaa gcgggaaata agaatttcat
    15901 agtcagaagg acgaccttcg cggcgtaaaa aacctccggg aaaacgtcca aaagcagcga
    15961 atttttcctt gtaatctacc gataacggca taaaatcggt tccttcattg gcatcctgag
    16021 ccgaacatac cgttgccagc aacatggtat cacccatgcg taccattacc gatccatcag
    16081 cctgcttggc caatttaccg gtttcaataa caatttctcg tccatcactc attgtgatgg
    16141 tttttgtaac aactgtcata caattttttc ttttaaactt ttatttttcg gatacaaaga
    16201 tacaatttta tttgcaaatt ttttaatcct taataattta cagtcataag taacttatag
    16261 taacgtaaat tcaagtaatt aatgcaactt tttcagggga aataaaaggt aaagaaaaat
    16321 tttttctcaa aaacaaggga aaaaataaaa ggggtatcta attcggaaaa aattttgata
    16381 cccctttgtt atttatattg gcatagaaca aaaaatcaaa tttctttcac cagttctttc
    16441 attatcagtg tcataagtgg ctgcaccgaa tctccgattt tttgaacttc ttcgtgagaa
    16501 acttccacaa tttttcccgg tacacccaaa tcggtaataa tggaaatacc gaaacatttc
    16561 atgcctgcat gattggccac aattacttcg ggaacagtgc tcatgccaac tgcatcgccg
    16621 ccaatggcac gaaaataccg gtactcggca ggagtttcaa aagtaggtcc actggttcct
    16681 acataaacgc cttccaccac tttaatgtga tttcgttctg caatttgttt ggcctttaca
    16741 ataaaatcct tgcaataagc ttcactcata tccgggaaac ggggtccgag ctcttcaaaa
    16801 ttttttccac gcagcggatg ttcgggaaac aaattgatgt gatcggtaat aatcatcaaa
    16861 tctccaattt cgaaatcggg atgcatacct cccgcagcat tggaaacaag taaattttta
    16921 atacccagtg ctttcatcac tcttacggga aaagttacgg ctttcatgtc gtacccttcg
    16981 tagaaatgaa aacgtccctg catggccaac acttctactc ctccgagttt tccgagtatc
    17041 agcttgcctt tgtgtccctc aacggttgaa acgggaaaat tgggaatggt ttcgtaaggt
    17101 atttccattt caatatccat ttgggttacc aaatttccca atcctgttcc caaaatgata
    17161 cctgttttag gtcgcattgg cattttggcc aacaaaaaat cagcggtttc ttgaattttc
    17221 tctaacataa tctttaagtt tttgaatgaa taatgattct tgatcgaata aaaatttaac
    17281 ctctatcggc aaggcaaaca tttttgcctt gagcgtttcg ggtaaatatt tgtcgttata
    17341 aagtcgtgcc gcatcttttt ccgtaacgat aataatttta ttttcggacg gaagagcgtc
    17401 ccattcctgt cggatcaatt ggaagtcatt ttttgaaaat ttgtgatggt cggggaagga
    17461 aacttctttc aaagtcgaaa cataatcttt cagaaaatcc ttcatcgatt gaggagaagc
    17521 aatgccggta accatcaaaa cggccggact ttcttccctc aatttgctca atgtccaatt
    17581 ttcagtagtc aactccggaa aaaccggcac cggatcgtga taccgaaaag tagaaaaaaa
    17641 cacttgctga taaggcaatg gatgcagctc catttcaata atgcgtttgt cgatgggttt
    17701 gaggttttcc gggcatttgg taaccactat caggttggcg cgatggctgc cttccttgct
    17761 ttcgcgcaaa ctgccggccg gcagaaaaaa atcggatgtg taaaggttcg aataatcggt
    17821 gagcaaaacg gaaaaacccg gaagcaaata acgatgttga aaggcatcgt ccaacacgat
    17881 caattgcaaa tcgggaaaaa gtttcaacag ccgtttgata ccctctactc ttttttcgtc
    17941 cactgccacc ttaacgttgg gaaattttcg gaaaatttga aaaggttcgt cgccaatggt
    18001 agcggcagag gcattttcgt ccgccaaaac aaaacctttg gtttttcttt tatatcccct
    18061 gctgagaaaa gcagttttaa attcatgctg caggtgcgac aaaacaaatt ctgcatgtgg
    18121 cgtttttccc gtcccgccaa cagccaaatt tcccaacaca atggttggca catcgaaccc
    18181 gacggaatga aaaatttttt cgtcaaatag ccagttgcga atatcgacaa tcaatccgta
    18241 aagaagggac agcggataaa gaacaatatg tttcaggtct tttttcaatt tgggattgtt
    18301 ttttgaaaaa gaagagagca aagataccat ttttctgatt tgaaacaaac tgaaacttac
    18361 aaatcattca acaaggaaaa aagaagaaga aacacgaaaa cgggaaaggt aaaaaaacgc
    18421 tgaatattgc tgataataaa attttagtgt accttcaggg aacaggatcg tagccgctgc
    18481 ctccccaagg atggcaacga cttatgcgtt tcactgaaag ccaaccgcct ttgaaaacgc
    18541 catatttttt taccgcatcg atggtatatt gcgaacaggt gggcgtgtac cgacaacttg
    18601 gcggaaaaag cggagaaata ctgtaccggt aaaaatagac cggcagcaat accaaccatc
    18661 gaaagaattt tttcattgct cagaagctat ttttttcaac tgatccaaaa tctgtttcat
    18721 tttgtcgttc agttcatgga agtttagttc cttatctg
//
LOCUS       DAOTAX010000040        18700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2456_length_18700_cov_31.629391, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000040 DAOTAX010000000
VERSION     DAOTAX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18700
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2456_length_18700_cov_31.629391"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..500)
                     /locus_tag="PLM04_05535"
     CDS             complement(150..500)
                     /locus_tag="PLM04_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02572.1"
                     /translation="MRIPLCAKLIESMCKWLVDNGFENTPLHFSRFFPTNQLPDLSPT
                     PIKTLLNAREIAQTAGIKYVYVGNVPALGYENTFCTSCKKLLIERVGYTVNKNFINDG
                     KCLFCRTPIPGVWQ"
     gene            complement(603..1976)
                     /gene="xylE"
                     /locus_tag="PLM04_05540"
     CDS             complement(603..1976)
                     /gene="xylE"
                     /locus_tag="PLM04_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HON02573.1"
                     /translation="MNKKQENKVFVFGITLVATLGGLLFGYDTAVISGAEKALQAYFH
                     TSNLIHGITTSSALIGCIIGGSVSGVFASKLGRRKSLQVASVLFFLSALGSGYPEFLF
                     FKQGSASLDVLIAFNIYRIMGGIGVGLASAISPMYIAEIAPADIRGKLVSWNQFAIIF
                     GMLVVYFVNYSIAVGQSIEWINVIGWRRMFASEAIPASLFGILLFFVPETPRYLVLNN
                     KEDKAFNVLKKISGIDEGSKILEDIKATVHETTGKVLSYGWKILIVGILLSVFQQFVG
                     INVVLYYAPRIFQSMGAARDASMMQTVVMGFVNIVFTLVAILTVDKFGRKPLLIVGSI
                     GMAIAMFAISGLAYAEIIGVSTLVFIIIYTASFMMSWGPICWVLISEIFPNTIRSKAV
                     AIAVAAQWIANFLVSSTFPSLINFDPAFTYALYGCMAVLSAIFVWRMVPETKGKTLEE
                     MSKLWKK"
     gene            complement(2037..3353)
                     /gene="xylA"
                     /locus_tag="PLM04_05545"
     CDS             complement(2037..3353)
                     /gene="xylA"
                     /locus_tag="PLM04_05545"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HON02574.1"
                     /translation="MTIKEYFPTVGKIKFEGKESKNPFSFRYYDAEKVVYGRKMKDWF
                     KFSMAYWHTLCAEGSDQFGGPTKSFPWNEGKDALERAKNKMDAGFEFMQKVGINYYCF
                     HDIDLIDEGNTIEEYEANMKAIVEYAKQKQAETGIKLLWGTANVFSHKRYMNGAATNP
                     DFNAVARAAVQIKNALDATIALGGENYVFWGGREGYMSLLNTQMKREKEHLAMMLTMA
                     RDYARSKGFKGTFLIEPKPMEPTKHQYDVDAETVIGFLRAHGLDKDFKLNIEVNHATL
                     AGHTFEHELQCAADAGLLGSIDANRGDYQNGWDTDQFPIDIYDLTQAMLVILQNGGLQ
                     GGGTNFDAKTRRNSTDLEDIFIAHIAGMDAFARALESAAAILEKSPYKKMLTDRYASF
                     DSGKGKEFEEGKLTLEDLRTYALSKGEPALMSGKQELYEAIVNMYI"
     gene            complement(3445..4923)
                     /locus_tag="PLM04_05550"
     CDS             complement(3445..4923)
                     /locus_tag="PLM04_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HON02575.1"
                     /translation="MYLLGYDIGSSSIKASLINATTGECVSSEFFPKKEMQIKSVKPG
                     WAEQDPETWWENLKLATQAVITDSKVRSEEIKAIGISYQMHGLVLVDERLQVIRDAII
                     WCDSRAVPYGEKAFKEIGEEKCLSRLLNSPGNFTLAKLAWVKENEPENFKRIKYYMLP
                     GDYIALRLTGKPCTTASGLSEGIAWDFTKEAPADFLFDYFGFSTSIIPEIVPTFGNQG
                     FLSAEAAHELGLAAGTAVTYRAGDQPNNALSLKVLNPGEIAATAGTSGVVYGVNGEIK
                     YDPQSRVNSFAHVNHSAGEKRIGVLLCINGTAILNAWIKNNVAPEGVSYNEMNEMAAK
                     IPIGSGGVSIIPFGNGAERMLGNREPNCSIHGINFNHIGKAHLIRAAHEGIAFSFKYG
                     MDIMNEIGIETKTIRAGHANLFLSPVFRQTLANITGATIELYNTDGSIGAARGAGIGA
                     EIYKNEEEAFATLKKILTVQPEKENQEITREAYDRWLQHLKF"
     gene            complement(4968..5678)
                     /locus_tag="PLM04_05555"
     CDS             complement(4968..5678)
                     /locus_tag="PLM04_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HON02576.1"
                     /translation="MESVNVYNPHVSVDCVVFGFDGEKLKVLLIERTIDEPGEQYNDK
                     KLPGSIIYENENLDEAASRVLGELTGLKNIYLSQFKSFGDPKRTSNPRDIYWLENTTK
                     MKIGRIVTVGYVALIKINRKIKLEEDNKTVQWYDVKDIDKINLAFDHNEIIHAALEHI
                     RHTLKVEPRLMFELLPRKFTMSQLRILYDVIHQTHSDVRNFQKKMMQLHQLVMLDEVE
                     KNVPHRAARLYKFDRKKL"
     gene            complement(6339..7214)
                     /locus_tag="PLM04_05560"
     CDS             complement(6339..7214)
                     /locus_tag="PLM04_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HON02577.1"
                     /translation="MYKIIVVLVGFMSFFSSVQGQLLWKISGNGLKKSSYLFGTHHFI
                     PLQFLDSIPGLYQAFNTSEIVVGEMVVNNLDAVEKVKKAAIIPNQQTIYDLVDKNQIL
                     QIDSALKSVLHIGLKEMALLQPNLILTIYQTEVYKKASGQIENIAPDSYFQQLGIAKG
                     KKIVGLETIDQQLEMLFDKTSLQRQAEIFTYSVLHTDNMILETNKLNNFYKKGDLEKL
                     LLLSIQKNNPSDATAEEYYKLVDERNLEWMKQLPELIKSSSCFITVGALHLPGENGLI
                     EQFRKLGYHVTEVKK"
     gene            7439..9676
                     /locus_tag="PLM04_05565"
     CDS             7439..9676
                     /locus_tag="PLM04_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HON02578.1"
                     /translation="MSNIYLIIILVLLGLAVLDLVVGVSNDAVNFLNSSIGAKVAPLW
                     IILTVASAGILLGSLFSGGMMEIARSGVFFPAKFTFPEVMMLFLAVMITDVILLDVFN
                     TFGLPTSTTVSLVFELLGSAVAVSLFKIWHSDAGAALSEYINAGKSITIISGIFISIA
                     IAFLCGTVIMYISRIIFSFNYKASFNYFGALWSGLAFTGIAYFVIFKVLLTSTLVSSE
                     TAAYINANIGTFLWGIFLASTVILGLLQHLFKINILKIVVLAGTMSLAMSFAGNDLVN
                     FTGPFMAALNSFQIASGVAASGGSIDNLYMGMLSEPVMADWRYLTASGIIMILALWFS
                     KKAKTVTKTEINLARQGEGIERFSSTQASRTLVRRAINLSRLIEKITPKKVKDFIESR
                     FEVVPLNDKDAPSFDLIRASVNLTVSALLISLGTSLKLPLSTTYVTFMVAMGTSLADR
                     AWGRESAVYRITGVLTVIGGWLMTALVAFTVSLIVGLTLMYGKVYAVYGLLLLVLILL
                     IQFASVHRKREKKEAAKSSTILTNEDAILHECTELVKRTIETTIRIFRETIDALHTED
                     RKKLRNLYKEADNLNNEWKDKRNYEVLPTLQHLQSGALEIGQYYVQIVDFSHEISKSL
                     RVITEASHKYIENNHEPFSQEQLNDLMTVCDAFVDVFNEYAKMVETNNFTSYANIRDK
                     QLRISELFSELTKKQIKRVKANESGTRNSILFLNILNEAKLISLQINNLMRARFRLFS
                     LSDKN"
     gene            complement(9782..10228)
                     /locus_tag="PLM04_05570"
     CDS             complement(9782..10228)
                     /locus_tag="PLM04_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HON02579.1"
                     /translation="MEKSKLMNEKQKLLDMRYMRMARIWAENSYCRRRQVGALLVKNK
                     MIISDGYNGTPAGFENNCEDEDDNSKPYVLHAEANAITKVARSHNSSDGATLYVTASP
                     CMECAKLIIQAGIKRVVYGEHYRIMDGIELLQRAGIEVVYLGDDNI"
     gene            complement(10234..11601)
                     /locus_tag="PLM04_05575"
     CDS             complement(10234..11601)
                     /locus_tag="PLM04_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HON02580.1"
                     /translation="MQRVHFIAIGGAAMHNLAIALSKKNNYKITGSDDEIFEPSYSRL
                     KEHGLLPEKTGWFPERIHKGLNAVILGMHAREDNPELLKAKELKLKIYSFPEFLYQQT
                     RNKTRIVVGGSHGKTTTTAMILFVLNKLKIEADYMVGAQIEGFDNMVRLSYDSRIAVF
                     EGDEYLTSPLDPRPKFHLYHPHIAVLTGIAWDHMNVFPTFENYLEQFKKFTDLIELQG
                     RLIYFDGDENLNKIVQQLRRDIVPFPYHTPPYEVRNEITWLKIKNKEIPLKIFGEHNL
                     QNLTAAHIACHQIGISDDDFFTAISEFSGASNRLEKLTENNDTVVFKDFAHSPSKVKA
                     TIKAVRQQYPHRRLIACLELHTFSSLMEDFLPQYARCMDEADVAMVYYDPEIIEQKRL
                     KQFEPEQVKTSFGKADLQVFTNKNDLEKALKSLDYKNSALLLMSSGNFSGLNVNDIVN
                     EKISL"
     gene            11702..12544
                     /locus_tag="PLM04_05580"
     CDS             11702..12544
                     /locus_tag="PLM04_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON02581.1"
                     /translation="MKIHKIEAGFFDCDGGAIFGVVPKKVWQKRYPCNEENFCKLAMR
                     CLLIDTGEKLVLIDSGTGDKQLDYLKYYNFKGVVNFELALQQLGYSCGDVTDVILTHL
                     HFDHCGGCTYYDSDRKNVLLTFPNAVHWVGERQWINFLEPNVREGDSYFPENMLPVQQ
                     AGKLKLVKENSFLCPEIELRIFNGHTVGQLVSYIQYLGKTWIYVGDVIPVAPSIPLAW
                     ISAFDAYPVTSLEEKKILLQEAEEKHQILLFEHDAYTECCTVKKSNDNFRMDESFPFP
                     NPVK"
     gene            complement(12765..14198)
                     /locus_tag="PLM04_05585"
     CDS             complement(12765..14198)
                     /locus_tag="PLM04_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02582.1"
                     /translation="MSNFRWSIVSLLFFATAINYLDRQVLSLTWKDFIAPEFHWTNTH
                     YGTITSLFSLFYAIGMLFAGRFIDKLDTKKGFLWAIGIWSLGAVLHAFCGIATSGIVA
                     GKWLVGFEGAKEAIATVNNVSMVLNVSVTLFIFARLVLAIGESGNFPASIKAVAEYFP
                     KKDRAFATSIFNAGSTVGALAAPVVIPVIAEKWGWEMSFIIIGALGFIWMGFWAFIYK
                     KPEVHPKVNKLELEYIHLDHADEAAVNPNANTDKEKKLSFKQCFKFKQTWAFAFGKFM
                     TDGVWWFYLFWTPAYLSSVYGLKSSDPRSQLAIFVLYAITLLSIIGGWLPTYFVDKKG
                     MHPYAGRMKAMLIFAFFPLLALFAQPLGHISYWLPIIIIGIAGAAHQSWSANIFSTVS
                     DMFPKSAVATVTGIGGMAGGIGSMLINKASGALFDFAGQTQMSFLIFKGEEAGYFIVF
                     SFCAVAYLIGWIVMKALVPKYSPITDL"
     gene            complement(14276..14953)
                     /locus_tag="PLM04_05590"
     CDS             complement(14276..14953)
                     /locus_tag="PLM04_05590"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HON02583.1"
                     /translation="MMKFNKLEVLSTIKSTGVVPVFYHSNFEIAKSVVKACYEGGIRA
                     FEFTNRGEFAHEVFGQVVKFVQKDCPDMIMGVGSVIDAPTAALYIQLGANFVVGPLFN
                     PDVAKICNRRLVPYMPGCGSVSEIGFAQEAGCDVCKVFPGEVLGPAYVKALKAPMPWS
                     NLMVTGGVKPEEANLKSWFDAGVMCVGMGSNLFPADVIAAHDWGKITELCRETLDLIK
                     KIQKNIK"
     gene            complement(14997..16040)
                     /locus_tag="PLM04_05595"
     CDS             complement(14997..16040)
                     /locus_tag="PLM04_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HON02584.1"
                     /translation="MSKKIVTFGEVMLRLATPGYERFSQATEFSATFGGGEANVAVSL
                     ANYGMETEFVTRLPQNEIAESCIMDLRKHNVGTHYILRGGERMGIYFLETGAVARGSK
                     VIYDRAHSSIAEIEPGMIDWREVLKDADWFHWTGITPAISQGTADVCLEAVKVANELG
                     VTVSTDLNYRKNLWKYGKSASEIMPELVEGCDIILGNEEDAEKVFGIKPEGFDVSRTG
                     GEIDSAEFESVCKQLMKKFPRAKKVIITLRGSINANHNTWGGVLYSGEKLYQSKRYDI
                     THIVDRVGGGDSFMGGLIYGLLTYEGNDQKALDFAVAASCLKHTIYGDYNLVTVKEVE
                     ALMSGDESGRVSR"
     gene            16442..17518
                     /locus_tag="PLM04_05600"
     CDS             16442..17518
                     /locus_tag="PLM04_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON02585.1"
                     /translation="MEKAKGKIRIKDIARMAGVSEGTVDRVLHQRGVVSEKSRKAVKK
                     VLEEIDYTPNLLARSLASKKKYRFICLFPAYNKGEYWESIEKGFRQASEEFNNYKVEV
                     MIEYFDQFDSSSFLATAKAVFVYNPDAIIMAPVFRQETILFARELTEKKIPFSFIDSM
                     VENVEFTSYYGQHSLQSGYIAGKLLFNSLPKESKILVLHTYRKGGSGSIQTENRKKGF
                     LQYIEENNLKNFCEILSIEIIEGNDEENMRLIRNAFIQHPDIRAAITFNSKVHRLAKY
                     FKNLSHSDIKLIGYDVLEENVKCMKEGFVSFLIAQRPEKQAYCAVRDMSFHLIFDKEV
                     NKINYVPIDILMKENIDYYLNFNE"
     gene            17690..>18700
                     /gene="amrS"
                     /locus_tag="PLM04_05605"
     CDS             17690..>18700
                     /gene="amrS"
                     /locus_tag="PLM04_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HON02586.1"
                     /translation="MNTSIASDWIQPALYQQPLENGMVKCVLCPHQCMVHEGKTGLCH
                     TRQNINGVLFTLAYANPCSLNVDPIEKKPLFHFLPGSKIFSLATAGCNFKCLNCQNWE
                     ISQSSPQEVIHYDLLPEAVVELASNRQIASIAFTYTEPTVFYEYVYDTAKLAHQKNMK
                     TVIVSNGYINEKPLLDLCPFLDAANIDLKCFDNHLYRKLTGGSLEPVLKTLKTLKAQG
                     VWLEITNLIVPTYSDSPEMIESMCKWLVDNGFEDTPLHFSRFFPANQLPDLPPTPIKT
                     LLAAREIAQTAGMKYVYVGNVPALGYENTFCPSCKKLLIERTGFTVNKNFINDGKCLF
                     CHT"
BASE COUNT         5689 a         3731 c         3414 g         5866 t
ORIGIN      
        1 ttgctacatc agcctgttat tccttttttg aagtgtaccc cacaaaaata cacaataatt
       61 ttaattattc aaaacaaatg attaaaaaaa ttaattattt gtttaatcga ttggactatt
      121 gtggtttaag aagataaaat tcattcattt tactgccaaa ctccaggtat aggggtacgg
      181 caaaacagac atttgccatc gttaatgaaa tttttgttaa cggtatagcc aacccgttct
      241 atcagcaatt ttttgcagga agtacagaaa gtattttcgt agccaagtgc aggaacattt
      301 cccacataaa catattttat tcctgcagtt tgggcaattt ctcgagcgtt gagcaatgtt
      361 tttatgggcg tcggtgataa atcgggtaat tgattggtag ggaaaaatcg gctgaaatgg
      421 agtggcgtat tttcaaaacc attgtcaacc agccatttgc acatgctttc tattaacttg
      481 gcacatagag gaattctcat cctggcagtt taataccatg cccgacacac aaagaaaatc
      541 cactccggag aaattctttt atttccggag tggaatcaat cacaaaaaac caactaaatt
      601 cactattttt tccacagttt gctcatttct tccagcgttt ttcctttggt ttcgggcacc
      661 attcgccaaa caaatattgc cgataaaact gccatacatc catacaaagc ataagtaaat
      721 gccggatcaa aattgatcaa agaagggaaa gtggaagaaa ccaggaaatt ggcaatccat
      781 tgagctgcaa cggcaatagc aaccgctttg ctacgaatgg tattgggaaa aatttccgaa
      841 atcaataccc agcaaattgg tccccacgac atcataaagg atgccgtata aataataatg
      901 aaaaccaatg tactgacgcc gataatttct gcataagcca gtccgctaat ggcaaacatg
      961 gcaatagcca taccaatgga accaacaatc aacaaaggtt ttctgccaaa tttatctact
     1021 gtcagtatgg ctaccaacgt aaatacaata tttacaaagc ccattacaac tgtctgcatc
     1081 atggaagcat cgcgggcagc gcccatactc tgaaaaattc gcggagcata atacaatacc
     1141 acatttatac ctacaaattg ctgaaaaaca gatagaagaa taccgactat caaaattttc
     1201 cagccgtacg aaagaacttt tccagtagtt tcgtgtactg ttgccttgat atcttccaaa
     1261 atctttgaac cttcgtctat acccgatatt tttttcagca cgttaaatgc tttgtcttct
     1321 ttattgttaa gaaccaaata acgaggcgtt tcagggacaa aaaacagcaa aattccaaaa
     1381 agcgatgcag gaatggcttc agaagcgaac atccttcgcc agcctatgac attgatccat
     1441 tcaatggatt gaccaacagc gatggaataa ttcacaaaat aaaccaccag cattccaaaa
     1501 ataatggcaa actgattcca tgaaacgagt tttccccgaa tatcggcagg agcaatttcg
     1561 gcaatgtaca tgggagaaat ggctgaagcc aaccctacgc ctattccacc catgatgcga
     1621 taaatattaa aagcaattaa cacatcaagt gaagcactac cttgcttgaa aaacaaaaat
     1681 tccggataac ctgagcccaa tgctgataag aaaaacaaaa ctgaagctac ttgcaaagac
     1741 tttcttcgtc caagtttgga tgcaaaaact ccggagaccg atcctccaat aatacaacct
     1801 atgagtgcac ttgaagtggt aataccatga attaaattgc tggtgtgaaa gtatgcttgc
     1861 aaagcttttt cagctcctga aatcacagcc gtatcatatc cgaagagcaa accacctaaa
     1921 gtggctacca atgttattcc aaaaacaaaa accttgtttt cttgtttctt attcataatt
     1981 ataaatttag ccatttaagc actttcaaaa agtaacttga atggggctga tgaattttaa
     2041 atatacatat taacaatagc ttcatacaat tcttgcttgc cactcataag agcaggttcg
     2101 cctttggaaa gtgcataagt gcgcagatct tccagtgtca atttaccctc ttcaaattct
     2161 ttaccttttc cactatcaaa agaagcataa cgatcagtga gcattttttt gtagggcgat
     2221 ttttccagaa tggcagcagc cgattccaat gcacgtgcaa aagcatccat cccggcaata
     2281 tgagcaataa aaatatcttc caaatcggta gaatttcgac gggttttggc gtcaaaattc
     2341 gttcctcctc cctgaagtcc gccattttgc aaaataacca gcatggcttg tgtcaaatca
     2401 taaatatcaa tcgggaactg atcggtatcc caaccgtttt gataatctcc acgattggca
     2461 tcaatcgagc ccagtaaacc agcatcggca gcacattgca attcatgttc aaaagtatga
     2521 ccggcaaggg ttgcatggtt cacttcgata ttcagtttaa aatctttatc cagaccgtga
     2581 gcacgcagaa aaccaataac cgtttcagca tctacatcat attggtgttt ggtgggttcc
     2641 attggtttgg gttcgatcag aaaagttcct ttaaaacctt ttgaacgcgc ataatcgcgt
     2701 gccatagtca acatcatggc caaatgttcc ttttcacgct tcatttgcgt attgagcaag
     2761 ctcatgtaac cttcacgtcc accccagaat acataatttt cgccaccaag agcaatcgtg
     2821 gcatccagcg cattcttgat ctgaacggct gcccgtgcta cagcattaaa atcaggattg
     2881 gtagcagctc cgttcatata tcgtttgtgc gaaaatacgt tggctgttcc ccaaagcaac
     2941 ttgatccccg tttcagcctg tttttgtttc gcatattcca caatggcttt catattggct
     3001 tcatattctt caatggtatt tccttcatca atcaaatcga tatcatggaa acaataataa
     3061 ttgataccaa ccttttgcat gaattcaaaa ccagcatcca ttttgttttt ggctctttcc
     3121 agtgcatctt ttccttcatt ccacggaaaa cttttggtgg gtccgccaaa ctggtcgctt
     3181 ccttctgcac acaatgtatg ccagtatgcc attgaaaatt taaaccaatc tttcatttta
     3241 cggccataca ctactttttc ggcatcgtag taccgaaacg aaaaaggatt tttactctct
     3301 tttccttcaa atttgatttt tcccactgtt ggaaaatact cttttattgt cataattcat
     3361 aaattattaa attcctcagc caaattagct taagaataag ttaaacattt tttattatct
     3421 tgtaattatc actaaaattt ctatttaaaa tttcaaatgc tgcaaccatc gatcgtaagc
     3481 ttcacgcgta atttcctgat tttctttttc aggctgaacg gtaagaattt tctttaacgt
     3541 tgcaaaagct tcttcttcgt ttttataaat ttccgctcct attcctgctc cgcgagcagc
     3601 acctatcgag ccatcagtat tatacaactc aatggttgca ccggttatgt tagccagtgt
     3661 ctgacggaaa acgggcgaaa ggaaaaggtt ggcatgtccg gcacgaatgg ttttcgtctc
     3721 gatacctatt tcattcataa tatccattcc gtatttaaac gaaaatgcaa tgccttcatg
     3781 agcagctctt attaaatgag ctttgccgat atgattgaaa ttgatgccat gaatggaaca
     3841 attgggttcg cgatttccca acatccgttc tgctccatta ccaaaaggaa taatacttac
     3901 gccgccactg ccaataggga tttttgctgc catttcattc atttcattat aactcacacc
     3961 ttccggagcc acgttatttt ttatccatgc attcaatata gctgtaccat taatacaaag
     4021 cagcacacca atacgctttt caccggcaga atggttgaca tgggcaaacg aattgactct
     4081 cgactgcgga tcgtatttta tttctccgtt cacaccataa acaacgcctg aagttccggc
     4141 agtagcagca atttcacccg gatttagcac ctttaacgac aaggcattgt ttggttgatc
     4201 gccggcacga taagtaacgg ctgttcctgc tgcaagtcca agttcatgcg cagcttctgc
     4261 actgaggaat ccctgattgc caaaagttgg aacaatttca ggaataatgg aagtactaaa
     4321 cccaaaataa tcaaacagaa aatcggcagg agcttccttt gtgaaatccc atgctattcc
     4381 ttccgacaag cccgatgcag tagtacaggg ctttcctgtc agtcgcaaag caatgtaatc
     4441 gcccggcaac atgtaatatt tgattcgttt gaaattttcc ggttcatttt cttttaccca
     4501 agccagtttt gcaagcgtaa aatttcccgg agaattcaat aagcgagata agcatttttc
     4561 ttctccaatt tccttgaacg ctttttctcc gtaaggtaca gcacgactgt cgcaccagat
     4621 aattgcatcc cgaattactt gcagtcgttc gtccaccaat accaaaccat gcatctgata
     4681 ggaaatgccg atggctttaa tttcttccga ccgaacttta ctgtctgtta ttacagcttg
     4741 cgttgcaagt ttgagatttt cccaccacgt ttccgggtct tgttctgccc atccgggttt
     4801 caccgattta atctgcattt ctttcttcgg gaaaaattca ctcgaaacac attcgcctgt
     4861 tgtggcatta ataagacttg ccttaatgga ggaacttcct atatcatatc caagtaaata
     4921 cataattctt agtcgattat tgatttataa taatgatttt ttcaggttta taatttcttt
     4981 ctgtcaaatt tatacaaccg ggcagcacgg tgaggtacat ttttttctac ttcgtcgagc
     5041 atgacgagtt gatgcaactg catcattttt ttctgaaagt ttctcacatc cgaatgagtt
     5101 tgatgaataa catcatagag aattcttaat tgtgacatgg taaattttcg gggcagcaat
     5161 tcaaacataa gacgaggctc tactttcaaa gtatgccgaa tatgttccaa agccgcatga
     5221 atgatttcat tgtggtcgaa agcaagatta attttgtcga tatctttcac atcgtaccac
     5281 tgcaccgttt tgttatcttc ttccagctta atttttctgt ttattttgat taaagccaca
     5341 tagccaaccg ttacaatacg cccgattttc attttcgtgg tattttcaag ccaataaata
     5401 tcgcgtggat tactggttct cttgggatca ccaaaactct taaactgact gagataaata
     5461 ttcttcaatc ccgtgagttc tcctaaaaca cgactggcag cttcgtccaa attttcattc
     5521 tcgtaaataa tgctccccgg cagtttctta tcattgtatt gctcaccggg ttcgtcgatg
     5581 gttcgctcaa taagcaaaac tttcagtttc tctccatcaa atccgaaaac cacacagtca
     5641 accgagacat gcggattata aacatttact gattccattg atttataaag aagtttttga
     5701 ataacgacac aaaaataata tttgatttta aaataatagc ataagatatt atgttataaa
     5761 acacatttac aatatattaa acattgtatt ttgtacaata tgtaattgaa tcatattgta
     5821 taaagtacaa taataccaat gaaaacaaca aaatttattt attgaaaaaa aggcacagca
     5881 aaatatttcg tctgtttact taaaagattt tcagcttttt acaatgaaat aataaaactt
     5941 ttttcttttt tgggagaaat gccaaataac ttttgggaat agaaaaattt tttctgaaaa
     6001 aacaataaaa agaaagagtc ttctcttaaa aacctaattt ttttaaagtt tcattcggct
     6061 ttaatgtagt agacataagc tgccaagact tggcaatgcg ttctttgata gatgataaat
     6121 atcgattgtg agttattgac tttcgcatat accaccaaac acgttcaaca ggattcaagt
     6181 ccggagaata tgccggaaga tatcttattt ccaatgtggg attagcttcc agaaagccct
     6241 taagcttttt tgcatgatga aacctcacgt tatctactgg atttaccttt ggacaactga
     6301 ttgtgtaata atttaaggta atatatataa ttgttgcctc atttttttac ctctgttacg
     6361 tgatatccca attttctgaa ttgttctatc aggccgtttt cgccaggcaa atgcagtgct
     6421 ccgactgtaa taaaacatga tgagcttttt atcagttcag gtagctgttt catccattca
     6481 agatttcttt catccactaa tttataatat tcttcagctg tagcatctga aggattgttt
     6541 ttttgaatcg acaataaaag caatttttct aaatcaccct ttttgtaaaa attattcaat
     6601 ttgttggttt ctaaaatcat gttgtcggta tgaagaactg aataagtaaa tatttctgcc
     6661 tgtcgttgca gggaagtttt gtcaaaaagc atttccagtt gctggtcgat ggtttcgagt
     6721 cctacaattt tttttccttt tgctattcct aactgttgaa aatacgaatc gggtgcaata
     6781 ttttcaattt ggccggaggc ttttttgtat acttcggttt gataaatggt taaaatcaaa
     6841 ttgggctgca gcagggccat ttctttcaat cctatgtgaa gaaccgattt tagagctgag
     6901 tcgatttgca agatttgatt tttatctact aagtcgtaaa tagtttgctg gttgggaata
     6961 atagctgctt tcttaacttt ttccaccgca tccaggttgt tcaccaccat ttctccaacc
     7021 acaatttcag aagtattaaa tgcttggtat aagccaggga tactatcgag aaattgtaac
     7081 ggaataaaat gatgggtgcc aaacaaataa gaactttttt tcaatccgtt gcctgagatt
     7141 ttccataaca actgtccttg aacggatgaa aaaaacgaca tgaatcctac cagaacaaca
     7201 attattttat acatgaaatg ttaaattata tggttggcac aaatatatgc aaattatttg
     7261 ttgagataag gaaacaaaat caggcaacta ctgttttttg tgttttttaa ttgaaattac
     7321 gatttctatc aattcacatt tgtgttttgt aacatttttg aaatatcttt ttaatggatt
     7381 tgaaatttgt gttataaaaa acgatagtta tctttgtgca aaattattaa tttctaatat
     7441 gagtaacata tacctgatta tcattttagt attgttagga ttggcggtgt tggatttggt
     7501 ggttggagta tcgaatgacg ctgttaattt tcttaattcc agtattgggg ccaaagttgc
     7561 tccgctttgg ataattctta ctgttgcttc tgccggtata cttttgggtt ctttattttc
     7621 aggtggtatg atggaaattg ccagaagtgg cgtttttttc cctgcaaagt ttacttttcc
     7681 tgaggttatg atgttgtttt tggccgttat gattaccgac gtaatcctgc tcgacgtatt
     7741 caatactttt ggtttaccta cttctaccac tgtttctctt gtatttgaat tacttggttc
     7801 ggctgttgca gtttctttat ttaaaatttg gcacagtgat gcaggtgctg cattgtcaga
     7861 gtatattaat gcgggcaagt cgattaccat tatttctgga atatttatct ccatagccat
     7921 tgctttttta tgcggaacag taattatgta catttcacgc attatttttt ctttcaatta
     7981 taaagcttct ttcaattatt ttggtgcatt atggagtggg cttgctttta cgggcattgc
     8041 ttactttgtt atttttaagg ttttgttgac ttctactttg gtatcttcag aaacggctgc
     8101 atatataaat gccaatattg gtactttttt gtggggtata ttcttagcga gtactgtaat
     8161 tttaggactt ttacaacacc tttttaaaat aaatattctc aaaatagtag tactggctgg
     8221 taccatgtcg ttggctatgt cattcgcagg caatgatttg gtgaatttta ccggtccttt
     8281 tatggctgct ttaaattctt ttcaaatagc ttcaggtgtg gcagcaagcg gaggttctat
     8341 tgataatctt tacatgggta tgcttagtga accggtaatg gccgattggc gatatttgac
     8401 tgcttctggt ataataatga ttcttgccct gtggttttca aaaaaagcca agactgttac
     8461 caaaacagaa atcaacctgg caaggcaggg cgaaggaata gagcgttttt cttcaactca
     8521 ggcttcgcgt acacttgtga gaagagctat caatttatca agattgatag aaaaaattac
     8581 acccaaaaag gtcaaagatt ttattgagag tcgttttgaa gttgtacctt tgaacgataa
     8641 agatgctccc tcttttgacc tcatcagggc ttcagtaaat ttaactgttt ccgctttgct
     8701 tatttcttta ggtacatcat taaagctacc tctttctact acttacgtta cttttatggt
     8761 ggctatggga acttcacttg ccgatcgagc ttggggaaga gaaagcgccg tttatcgcat
     8821 tactggggta cttacggtta ttggtggctg gttaatgact gctctggtag catttaccgt
     8881 ttccttgata gtgggattga cccttatgta cggaaaagtt tatgctgtct acggattatt
     8941 gcttctggtt ttaattttat taattcagtt tgcttctgtt cataggaaaa gagagaaaaa
     9001 agaggcagca aaaagttcaa ctattttgac taacgaagac gcaattctgc atgaatgtac
     9061 cgaattggtg aaacgaacca tcgaaactac tattcggatt tttagggaaa ccattgatgc
     9121 acttcataca gaagacagaa aaaagctcag aaatctttac aaggaagcag acaacttgaa
     9181 taacgaatgg aaagacaaaa ggaactatga agttttgcct acccttcagc atcttcaatc
     9241 cggtgctttg gaaataggtc agtattatgt gcagattgtc gatttttcgc atgaaatatc
     9301 caaatcgtta cgtgtcatta ccgaagcaag tcacaaatat atagaaaata atcatgaacc
     9361 tttcagccaa gaacaattaa acgacctgat gaccgtttgc gatgcttttg tagatgtttt
     9421 taatgaatat gcaaaaatgg ttgaaacaaa caatttcacc agctatgcta atatacgaga
     9481 taaacaactc agaatttcgg aattgttttc cgaactgaca aaaaaacaaa ttaaaagagt
     9541 caaagcgaat gaatcgggaa cgcgtaacag tatattgttt ttgaacatac tgaatgaggc
     9601 gaagcttatt tcgttgcaaa tcaataacct gatgagagcc cgttttagat tgttttcttt
     9661 aagtgataaa aattaaagca gtattttaat ttgtcagtcc gtcgtgccac agataggctt
     9721 ccgtttatcg taaaggttta aaataatttt tgtaatccat aatacaaaaa agaggtgaat
     9781 tttatatgtt atcatcgccc agataaacca cctcaatgcc ggcacgttgt aaaagttcga
     9841 tgccatccat gattcggtaa tgttcgccat acacaactct tttgatgcct gcctgaatta
     9901 ttaatttggc gcattccatg cagggagagg cggtaacgta aagcgttgca ccgtcgctgc
     9961 tattgtgcga acgtgctact tttgttatgg catttgcttc agcatgaagc acgtaaggtt
    10021 ttgaattgtc gtcctcatct tcacagttat tttcaaatcc tgcaggtgtg ccattgtagc
    10081 catccgaaat aatcatttta tttttcacca gtaaagcacc cacctgcctg cgacgacaat
    10141 aggagttttc tgcccatatt ctggccatgc gcatataccg catatccagc aatttttgtt
    10201 tttcattcat aagttttgat ttttccatct gtttcacaat gaaatttttt cattgacaat
    10261 gtcattcaca ttcaaacccg aaaagtttcc cgacgacatg agtaaaaggg ccgaattttt
    10321 ataatccaaa gattttaaag ctttttccaa atcattctta ttcgtaaaaa cctgtaagtc
    10381 tgcttttccg aaacttgttt ttacctgttc tggctcaaat tgtttcagtc gtttttgttc
    10441 aataatttca gggtcgtaat aaaccatagc cacatctgct tcgtccatgc atcttgcata
    10501 ttgaggaaga aaatcttcca tcagactgct gaaggtgtgt agttcgaggc aggctattaa
    10561 tctgcgatga ggatattgtt gccgaacggc tttgatggta gctttaactt tggaaggaga
    10621 gtgggcaaaa tccttgaata cgacggtatc attgttttcg gtcaattttt ccaatcgatt
    10681 agaagcgccg gaaaattcgg aaatagccgt aaaaaaatcg tcgtcggaaa tgccaatctg
    10741 atgacaggca atgtgtgcgg cagtcaagtt ttgcaaattg tgttctccga aaatttttaa
    10801 aggaatctcc ttgtttttaa ttttaagcca ggtaatttcg tttcttactt cataaggtgg
    10861 tgtgtgataa ggaaagggga cgatgtcgcg gcgaagttgc tgaacaattt tattcaggtt
    10921 ttcgtctcca tcaaaataaa ttagtcttcc ttgcaattca attaaatcgg tgaatttttt
    10981 aaattgttct aaatagtttt caaaagttgg aaaaacattc atatgatccc aggcaatgcc
    11041 ggtaagtact gcaatatgtg gatgatataa atgaaattta gggcgtggat cgagcgggga
    11101 ggtaaggtac tcgtctcctt caaaaactgc tatgcgcgaa tcgtaagaaa gcctgaccat
    11161 gttatcgaaa ccttcaattt gagcgccaac catataatcg gcttcaattt tcagtttgtt
    11221 cagcacgaat aaaatcatgg ctgttgtggt ggtttttccg tgacttccgc ccaccacaat
    11281 acgcgttttg ttgcgtgtct gctgataaag gaattcaggg aaagaataaa ttttcaattt
    11341 taattctttt gctttcagca gttcaggatt atcttcgcgg gcgtgcatgc cgagaattac
    11401 agcattcaaa cccttgtgga tgcgctcggg aaaccagccc gttttttccg gcaacagtcc
    11461 atgttctttc aatcgggaat aagaaggttc aaaaatttca tcatccgaac ctgtaatttt
    11521 atagttgttt tttttactga gtgcgatggc taaattatgc atggctgctc cgccaatggc
    11581 aataaaatga actcgctgca taaggttgga aggtttttta aattttattg ctacttttgt
    11641 ggaaagtatt ttttataatg caaaaatacg aagaaaatac gaatcgaaag ctactttgaa
    11701 tatgaaaata cataaaatag aagccggttt tttcgactgc gatggtggag ccatctttgg
    11761 tgtagttccc aaaaaggttt ggcaaaagcg ttatccgtgc aatgaggaaa atttttgtaa
    11821 gctggccatg cgttgcctgt tgatcgatac aggcgaaaaa cttgttttga ttgacagtgg
    11881 tacgggcgac aaacagctgg attatttgaa atattacaac ttcaagggag ttgtgaattt
    11941 tgaattggct ctacagcaat tgggatacag ttgcggggat gttaccgatg taatcttgac
    12001 tcatttgcat ttcgaccatt gcggaggatg tacttattat gattcagatc ggaaaaatgt
    12061 attgctcacc ttcccaaatg ccgttcactg ggtgggagaa aggcaatgga taaattttct
    12121 ggagcccaat gtgagggaag gagactctta ttttcctgaa aatatgctgc ctgtgcagca
    12181 agcagggaaa ttgaaactgg tcaaggaaaa cagttttctc tgtcccgaaa ttgaacttcg
    12241 tattttcaac ggacatacag tagggcaatt ggtcagttat attcaatatt tggggaaaac
    12301 atggatttat gtgggtgatg taattcctgt agcaccatcc attccattgg cttggatatc
    12361 ggctttcgat gcttatccgg taacttcgtt ggaagaaaag aagattcttt tacaggaagc
    12421 tgaagaaaaa catcaaatcc tcctattcga gcatgatgct tatactgaat gttgtactgt
    12481 gaaaaaatcg aacgataatt tcagaatgga tgaaagtttt ccgtttccaa acccagtgaa
    12541 atagtctctt tttagtacta tagacagtac tatatagaaa atcagactgc catagtagtg
    12601 atatgttttt aaaaaatttt acggtaaagc attatcgctt tgtcttctgt ccggttgaat
    12661 gtcggacaga agacaaagcg ataaacttgt taaaagaatc attgagctta ctttaattgt
    12721 cgcttgactc tttatctaaa gaatttttgt cgtctcccaa ctgattataa atcggttatg
    12781 ggagaatatt tgggaaccaa agctttcata actatccaac caatgagata ggcaacagca
    12841 cagaaagaaa aaacgataaa gtaacccgct tcttcgccct taaaaatcaa aaatgacatt
    12901 tgtgtttgcc ccgcaaagtc aaacaaagca cctgaagcct tattaattaa cattgaacct
    12961 atacctcctg ccattccgcc tatgccggta actgtagcca cagcactttt aggaaacata
    13021 tcgcttacgg tagaaaaaat attagccgac catgattgat gtgcagctcc cgctatacct
    13081 atgataataa tgggcaacca ataagaaata tgacccaatg gctgggcaaa aagggccaac
    13141 aatgggaaga aagcaaaaat caacatggct ttcattcttc cagcataggg atgcattcct
    13201 tttttatcga caaaataggt cgggagccag cctccaataa tggacaaaag tgtaattgca
    13261 taaagaacaa atatagccag ctgacttctt ggatcggatg atttcagtcc gtacacagaa
    13321 ctcaaatatg caggagtcca gaaaagatag aaccaccaaa ccccgtctgt catgaatttt
    13381 ccgaaagcaa aagcccatgt ctgcttaaat ttaaaacact gtttgaaact cagtttcttt
    13441 tccttatccg tattggcatt aggattcaca gctgcttcat ccgcatgatc caaatgaatg
    13501 tactccaatt cgagtttatt cacttttgga tgcacttctg gttttttgta aataaatgcc
    13561 caaaacccca tccaaataaa acccaaagct ccgataatga taaacgacat ttcccaaccc
    13621 cacttttctg caatcacagg aatgacaacc ggtgcagcca gtgctccaac cgtagaacct
    13681 gcattaaaga tactggtggc aaaagcacgg tcttttttcg ggaaatattc tgctacagcc
    13741 ttgatagaag ccggaaagtt acccgattct ccgatggcaa gtaccaaacg cgcaaaaatg
    13801 aataaagtta cactcacatt cagcaccatc gaaacattat tcactgtagc aatagcttcc
    13861 tttgctccct caaaaccaac caaccattta cctgcaacga ttcctgaagt ggctattcca
    13921 caaaaagcat gtaacacagc tcctaatgac catattccaa ttgcccacag aaaacctttt
    13981 tttgtatcca acttgtcaat aaatcggcct gcaaacaaca tcccaatggc ataaaataac
    14041 gaaaataaag aagtaatggt accataatga gtattggtcc aatgaaattc aggagctatg
    14101 aaatccttcc acgtcagcga aagaacttgc ctgtctaaat aattgatcgc cgtagcaaaa
    14161 aacaatagtg agacgattga ccacctgaaa tttgacattt ttttagcttg ctgttccatt
    14221 tcaattgttt tacgatatta gaagttttac aatgtttgcc ttatgaagca aataattatt
    14281 tgatgttttt ttgaattttt tttatcaagt ctaatgtctc cctgcaaagt tcagttattt
    14341 ttccccaatc atgggcagca atcacatccg caggaaacaa attggaaccc atccccacac
    14401 acatgacacc ggcatcaaac cagcttttca aatttgcctc ttcgggtttt actccgcctg
    14461 taaccattaa attcgaccag ggcatgggcg ctttcaatgc ctttacatag gccgggccca
    14521 agacttcgcc ggggaaaacc ttgcaaacat cacaaccggc ttcctgagca aatccaattt
    14581 ccgaaaccga tccacatccg ggcatatagg gcaccaaacg ccgattgcaa attttagcaa
    14641 catcgggatt gaacaaagga cctaccacaa aattggctcc caactgaata taaagcgctg
    14701 ctgttggagc atcgatcacc gaacctacgc ccataatcat gtcggggcag tctttttgaa
    14761 caaatttaac cacttggccg aaaacctcgt gcgcaaattc tcctctgttg gtaaattcaa
    14821 atgctctgat tccgccctcg taacaagctt tgaccacgct tttggcaatt tcgaaattgc
    14881 tgtggtaaaa caccggcacc actccggtgg attttatggt agaaagtact tccaatttat
    14941 tgaatttcat gattttattt ggtattttaa tatcctgatt gtaaaatttt aaaatgttac
    15001 cgggaaactc ttccgctttc atctccgctc atcaaagctt ccacttcctt gacggtaacc
    15061 aggttataat cgccgtaaat ggtatgtttc aggcaagagg ctgccacagc aaaatccagc
    15121 gccttctgat cgtttccttc gtaagtcaac aagccgtaaa tcagtccgcc catgaaagaa
    15181 tcgccgccgc ccactctgtc cacaatatgc gttatgtcgt aacgtttcga ttgatagagt
    15241 ttttcaccac tgtagagcac gccaccccac gtattgtgat tggcattaat ggatccgcgc
    15301 aaggtaatga tcactttttt ggcacgtgga aattttttca ttaattgctt gcaaaccgat
    15361 tcaaattcgg cagaatcaat ttctccgccg gtacgggata catcgaaacc ttccggcttg
    15421 ataccaaaaa ctttttctgc atcttcctca tttccgagaa taatatcgca accttcaacc
    15481 agttcgggca ttatttcgga agcggatttt ccgtatttcc aaagattttt tctataattc
    15541 aaatccgtag ataccgttac gcccaattca ttggccactt ttacggcttc gagacaaacg
    15601 tcggcagttc cctgcgaaat ggccggcgta atgcctgtcc agtgaaacca atcggcatct
    15661 ttcaatactt ctcgccaatc aatcatgccc ggctcaattt ccgcaatgga cgaatgtgcc
    15721 cgatcataaa ttaccttgct tcctcgtgct accgctcccg tttccaaaaa ataaattccc
    15781 atgcgttcgc cacctcgtaa aatgtaatgc gtacctacat tgtgcttgcg caagtccatg
    15841 atgcacgatt cggcaatttc gttttgtggc agccgcgtaa caaattcggt ctccattccg
    15901 taattggcca acgaaaccgc cacattggct tctcctccgc caaaagtggc actaaactcc
    15961 gtagcctgac taaatctttc atatcccggc gtggccaacc gtagcattac ttctccaaat
    16021 gttacaattt ttttgctcat ttttcagtct cctttttaat tgttgataaa taattggtcg
    16081 accaaaatta ctgcttttat attcactttc aaaatttttt tatttcgttt tcaaaaaaaa
    16141 tttttttcct tgaaaatctg cggaaaaaat aaaaagaaga cgaaattttt gaaattagtt
    16201 ttattaactt tgctcaatta attataagat aaaaatccgg cgaaaaacaa gaacaagtgc
    16261 taaaatttct tatattgcca taaagtaaaa agcaaatcat gaattgaaaa tcaggtttca
    16321 atgaaaattc tgtgacttgt tggcaaaagt acaaaacaat ttcatattat caaatattta
    16381 ttttaatttt ggacatattt tttttgtgca cgtacacaaa ataacaatta aaaagcaaga
    16441 tatggaaaaa gcgaaaggca aaatacgcat taaggatatt gccaggatgg caggagtatc
    16501 tgaaggtaca gttgacaggg tgttgcatca gcgaggtgta gtttccgaaa aaagtcgaaa
    16561 agcagtaaag aaagtattgg aagaaattga ttatacacca aatttgcttg ctcggtcgtt
    16621 ggcttcgaaa aaaaaatatc gttttatttg tcttttccct gcctataaca aaggtgaata
    16681 ctgggaaagt atagaaaagg gatttcgaca agcctctgaa gaatttaaca attacaaagt
    16741 agaggtaatg attgaatatt ttgatcagtt tgatagcagc tcatttttag caacggcaaa
    16801 agctgttttt gtctataatc ctgatgccat tattatggct ccagttttca ggcaagaaac
    16861 cattttattt gccagagagc ttacagaaaa aaagatccct ttttctttta ttgattctat
    16921 ggtagaaaat gttgaattta catcttatta tggtcagcat tctttgcaaa gcggatatat
    16981 tgcaggcaaa ttactgttta attctttacc gaaagaaagc aaaattttag tacttcatac
    17041 ctacagaaaa ggtggttctg ggtctattca gacggaaaat agaaaaaaag gatttttgca
    17101 atatattgaa gaaaataatt tgaaaaattt ctgtgaaata ttgtctatag aaattattga
    17161 gggaaatgat gaagaaaaca tgcgcttaat ccggaatgct tttatccaac acccggatat
    17221 tcgggcagcc ataactttta actctaaagt tcatagattg gccaaatatt tcaaaaacct
    17281 ttctcattcg gacattaaat taattggata tgatgtattg gaggaaaatg taaaatgcat
    17341 gaaagaaggg ttcgtttctt ttttgattgc tcaacgaccc gaaaaacagg cttattgcgc
    17401 agtaagagac atgagttttc acttgatttt cgataaagaa gttaataaaa tcaattatgt
    17461 gcctatcgac attttgatga aggaaaatat tgattattat ttgaatttca atgaatgatc
    17521 gaattaagcc catgaggatt aatcaagaga atcaagtagc gtttttgcag tgcaaatttt
    17581 taatctacgt tgccaaaacc aacaaaaata ttgttttggg aaaaagaagt ttggatattt
    17641 ttgaagaaat aatttccttt aaaaaaatca tttttaagaa gatttcatca tgaatacttc
    17701 tattgcttca gattggattc aacctgccct gtatcagcaa ccattggaaa acggaatggt
    17761 aaaatgcgtt ctctgtcccc atcaatgtat ggtgcacgaa ggaaaaaccg gactttgtca
    17821 tacccggcaa aacatcaacg gggttctatt tacgcttgct tacgctaatc cgtgttcact
    17881 gaatgtcgat ccaatcgaaa aaaaaccact gtttcatttc ctccccggct caaaaatttt
    17941 ttcattggct accgccgggt gcaatttcaa atgcttaaac tgtcagaatt gggaaatttc
    18001 tcaaagctca ccacaagaag tgattcatta cgatttgttg ccggaagcag ttgtggaact
    18061 tgcctcgaat cgacagattg ccagcattgc cttcacttat accgaaccga ctgtatttta
    18121 tgaatatgtt tatgacacgg cgaagttggc acatcaaaaa aacatgaaaa cggtgattgt
    18181 ttccaatggc tatattaacg aaaaaccttt gctggacttg tgtccatttt tggatgccgc
    18241 caatattgac ttgaaatgtt ttgacaacca tttgtaccga aaactcaccg gcggaagtct
    18301 ggagcctgtt ttaaaaaccc tgaaaacatt gaaggcgcaa ggcgtttggc tggaaattac
    18361 caatctgatt gttcccacgt attccgattc cccggaaatg atagaaagca tgtgcaaatg
    18421 gctggttgat aatggatttg aagatacacc gctccatttc agccgatttt tcccggccaa
    18481 tcaattaccc gatttacctc caacacccat aaaaacattg cttgcagccc gagaaattgc
    18541 ccaaactgcc ggaatgaaat acgtttatgt gggaaatgtt cctgcacttg gttatgaaaa
    18601 tactttctgt ccttcctgta aaaaattgct gatagaacga accggtttta ccgttaacaa
    18661 aaatttcatt aacgatggca aatgtctgtt ttgccacacc
//
LOCUS       DAOTAX010000041        18563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2474_length_18563_cov_31.386373, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000041 DAOTAX010000000
VERSION     DAOTAX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18563
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_18563_cov_31.386373"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1685)
                     /locus_tag="PLM04_05610"
     CDS             complement(60..1685)
                     /locus_tag="PLM04_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3843 family protein"
                     /protein_id="HON02587.1"
                     /translation="MSTKSTDRINLKQWLELKPYSQQTSTDKYYVELANKVKNVLTKK
                     QPSAFLNEYLNEDEIDILCCFLVSYLEDIVTGSNIWNTFTKKHFSLYKKYLPFFPLEN
                     YILNNVNMQDVNFLIWYFLNTIQQNFFISPINEEIMDMAFEVKNILSDEYKYAPENPI
                     LKSYYQLNRDETNYFSVRGLIELILFNTYLFYPDTYIRFKKENEKEQKNKQKSVDKDR
                     EAMLFYEIKDNFINTSNTSLLAMTGKEWAAELLGEKHPLYKDVINISPKVKGFFLYKG
                     QDKNNIFIEHIASGRKFEMTKKSYEHYNDLKKVDTILLIGIVKWRGEWWFSGTSSKMD
                     YNKKLIEDVKHTLAFKQTVNFLDFEEFEGNKLIKNQMMAFLNANNGNFIAFMKRSQVN
                     EFIEKVTNCYNDKLNISEDEKERFKKNAKQKKDLKLEKEDEISADELDDPCIVFFNPH
                     SGIEIAVGINSAFPMKSNPFFHPEKSETDFWQLLLSNFYSAELVQFCLDTCKNNLPFL
                     RKKVWKTYLKDIDFVLRFLKVDSYHSKPEITFL"
     gene            2041..3267
                     /locus_tag="PLM04_05615"
     CDS             2041..3267
                     /locus_tag="PLM04_05615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HON02588.1"
                     /translation="MYSNKKIKVAFFADILTRDHDGAIKTMYQLIDKVPEENFEFMFC
                     TGTPPKEKINHKILQIPSLTLPFNSNYKACLPHFSSNKIVKALEDFDPDVIHIATPSP
                     LGFFGLNFARKKHIPVLSIYHTNFLSYIQYYFNRLPFLIKPVESIMKKIYQSFYNFCN
                     MVYVPTKQMIIELKDSGVSHQPLKLWQRGIDSNLFNPSKKDMHYIQQITGNNKPCILF
                     ASRLVWEKNLETLFNIYDETEAQNLDVNFIIAGSGVAEQAARKRMKKAFFLGFLNHEE
                     LSKVYASSDVFVFPSISETFGNVVVEAMASGCVPVIARGGGSQSLVMNGVTGFLCNPY
                     DAREYVENIKVILNYPSLRESIQQNAIRFASNFNWNRLANEYFSDLQMLAYKSSPTFE
                     DNVVLTNELFPTNYAI"
     gene            3314..4321
                     /locus_tag="PLM04_05620"
     CDS             3314..4321
                     /locus_tag="PLM04_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HON02589.1"
                     /translation="MSKLKRYRKLIISVLMISVSVLIIRFISSIDFHILNMYLQRMPS
                     MFLLVIAISFVGYFFSSLGWKLCMGEEGMNISIWKIFIIRHIGEMLGAFNPTSIIAGD
                     SLKISYLVKSGISTKIGLSSILLMRMITVLSSIFLIVVSIFYLIFSKFGNGKTFVLFL
                     LIALVMISGFLMAKYLLDDHLYLGKTLECIKSKTNWSFLTSKTIDSCYEINTLASNYF
                     KHHKLNFLLAFLLLTLHWVCGALEFFIILQTLHVNISVIDAISMEVGVSLFKSLGAIV
                     PGQIGIEEYGNKIMLDVVAVKGNEIWFVVSLVRRSRQLFWLSVAGVFFWITKRKKFTL
                     Q"
     gene            4321..5484
                     /locus_tag="PLM04_05625"
     CDS             4321..5484
                     /locus_tag="PLM04_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HON02590.1"
                     /translation="MYEEILCITHKYPPFTGGMEKQSYELIQGLSSYYKVHIIAFEGK
                     ENKLLWFLKLKSRVKKLLKANPNIKLIHLNDGAMGVAALWLQKITQIPVVVTYHGLDI
                     TYPLTFFQKKLIPKLIKYKGTVCVSKFTSEQCIKRGFDPKTTFIVNNGVDLKLGEIPF
                     DPAIAQKLVEKYGVDVTDKKIIMATGRPVKRKGFSWFLKNVMPLLDNDVILILTGPLK
                     KSQTFFDIFLRCFPKMLRHKIQLLFGFPDDNKEVIEQLKKQRNAFHLGRVSNEDLMQI
                     ISLADLFVMPNINIPGDVEGFGLVALEASVRGTYVLASKVQGITDAVIDMENGTLLPC
                     ENAEAWADKIKEMLSDKEKLKILSEKGRNFTIQHFSWQKMVDEYIQVFNGFIH"
     gene            complement(5952..8516)
                     /gene="glgP"
                     /locus_tag="PLM04_05630"
     CDS             complement(5952..8516)
                     /gene="glgP"
                     /locus_tag="PLM04_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HON02591.1"
                     /translation="MSLKLNQVNEPRWKEINVKSFLPDGLKKLEEIAQNIFWVWRSDA
                     RHLFRELDKDAWAESGYNPVQLLNMLSYERLVEVEKDAHFMSNLDRIYNEFQAYLHQP
                     FAPDKPSIAYFSMEYGLTEVLKIYSGGLGILAGDYLKEASDCGVDLTAVGLLYRYGYF
                     TQSLSVDGDQIEIYDKQNFNTLPIVQVRDENGQAVVIEVPYPGRTVYANLWKVSVGRI
                     NLFLLDTDNEMNSEADRSITFELYGGDWENRLKQEIMLGIGGILTLKKLGIEKDVYHC
                     NEGHAALINLQRLADLVTEKKLSFHEALEIVRSSSLYTVHTPVPAGHDRFDEALFGKY
                     MKDYAALLNISWNDLMNLGRENPDDPNDKFSMSVFACNTCQEVNGVSWLHGKVSQEMF
                     QPIWKGYYPEELHVSYVTNGVHLPTWTTSEWKGVYEKYLGKDFYDDQSNMKLWQAIYE
                     VPDQVIWETRVKMKNKLIDYIRLQFQENWLKSQGDPSRIMSVLESMNPNALIIGFGRR
                     FATYKRAYLLFTDLKRLSKIVNDPSKPVQFIFSGKAHPADQGGKALIKRIVEVSRMPE
                     FLGKIIFLENYDMHLAKRLISGVDIWLNTPTRPLEASGTSGQKAQMNGVLNFSVLDGW
                     WLEGYVKGAGWAITEKRTYDNDAYQDQLDAATIYSMLENEIIPLYFAKNSRGISPEWI
                     QYIKDSIAQITPRFTTKRMIDNYVEQFYTKLSNRHALLKDNNFARAKEIAAWKQKIAK
                     GWDKIEVVSMDIPEGLLKNPQVSEVYHMNIVVDIKDINDKGIGIELVITKPDKDNKFY
                     LHDVEEFKLVKANGSLLYFSLEYQLNRAGLFQFGFRMFPKNEDLPHRQDFCYVKWI"
     gene            complement(8749..10395)
                     /locus_tag="PLM04_05635"
     CDS             complement(8749..10395)
                     /locus_tag="PLM04_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HON02592.1"
                     /translation="MLKPEYIFETSWEVCNMVGGIYTVLSTRAATLKEVYGDKLIFIG
                     PDIWIQKNNPLFVEDSRLFPGWKDAVQQQFQLNIRIGRWQIHGNPIAVLVDFMPLMLR
                     KDEIYGHVWLQTGVNSLPAYGDYHESSMFGYAAGMVIESFYRFFGLSLKNDVIAHFHE
                     WQTTFGIFYLKEHLPEAATIFTTHATTVGRSIASNHKPLYDYLQEYNGDQMAAELNVV
                     SKHSAEKTAAHLSDCFTTVSDITAAECRQLLEIKPAVVTPNGFEDDFVPKADTFIQKR
                     QEARTCLRKVAETLLGYSLQENALFVGTAGRYEYKNKGIDVFIESMRRLIECPHLNRE
                     IVVFIMVPAHVMGPRTDLLAALNHPNLKLHSWNLFTTHELYDYQNDHIMSAIRWFHFT
                     NQSSQKVKMIFVPSYLNGSDGIFNKKYYDLLIGLDVTIFPSYYEPWGYTPLESIAFSV
                     PTITSDLSGFGRWVAKQPQDITEGVGVVHRSDYNQYDATVKIAQMICQFSSLEQSQVE
                     NIRSIAASISLKALWENFIQYYYEAYNIALTQKNLRLGKH"
     gene            complement(10631..11239)
                     /locus_tag="PLM04_05640"
     CDS             complement(10631..11239)
                     /locus_tag="PLM04_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HON02593.1"
                     /translation="MKNLLFVSNIVLFIAVVVLFVLFFQIKPAKNAVQLSLKGDTLSS
                     QVLPIAYINVDSLLLKYRFAKDANESLIKKQEDSRLTINSRARELQNEMNEFQRKLEN
                     NAFLSRERAEQEHTRLLKKQQELQNLDGQLTQQLLQEQQKMSEELRDTINAFLKEYNK
                     DGRYEIIFSNTTGDNILFASKSYDITDEVVKLLNERFSNKKK"
     gene            complement(11323..11568)
                     /locus_tag="PLM04_05645"
     CDS             complement(11323..11568)
                     /locus_tag="PLM04_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02594.1"
                     /translation="MFYTLLITLLIVLLSVLLLGFRIFFIKSGKFPNIHIGGSKALQQ
                     RGIGCATTQDKEAQRNFHKGIDVSNLLTEISEQLKNE"
     gene            complement(11756..12331)
                     /locus_tag="PLM04_05650"
     CDS             complement(11756..12331)
                     /locus_tag="PLM04_05650"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HON02595.1"
                     /translation="MKFQLPELPYAQNALEPVISERTISFHYGKHHLTYVNNTNNLVV
                     GTEFENQDLETIVKKATGPLFNNAAQIWNHNFYFLSLTPDKGTKPSEKLLKAIEAEWK
                     SLDNFKIEFGKAATGLFGSGWAWLVKDASGKLSIVNESNAGNPMTKNLSPILTFDVWE
                     HAYYLDYQNRRADYVAALWDLVNWSIVSERF"
     gene            complement(12635..12719)
                     /locus_tag="PLM04_05655"
     tRNA            complement(12635..12719)
                     /locus_tag="PLM04_05655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12683..12685),aa:Ser,seq:tga)
     gene            complement(12885..15398)
                     /locus_tag="PLM04_05660"
     CDS             complement(12885..15398)
                     /locus_tag="PLM04_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HON02596.1"
                     /translation="MTVNYSEQLHNILLYSLQEAERLGNNYIGPEHLLLGIIRNGKGK
                     AIDILNHTQVDLSKIKQSIESQIRTDNEPTGEEILSLKSTERIKKIMELETRSLSSET
                     ADSEHLLLAILKEKNNLAVEVLNEENLTYEIAKSYLEHSKDIQMSSNFQDDDDEDDEP
                     RQHQRNVSGSGKQSDTPALDTFGTDITKAALENRLDPVVGRQVEIERLAQILSRRKKN
                     NPVLIGDPGVGKSAIVEGLALRIVQHKVSRVLFDKRIVALDMASIVAGTKYRGQFEER
                     IKAILNELAKNPNIILFIDEIHTIVGAGGAPGSLDAANMLKPALARGEIQCIGATTLD
                     EYRQSIEKDGALERRFQKIIVEPTSYEETLQILRNIQDKYEYHHNVRYTPAAIEACVR
                     LTERYITDRCFPDKAIDALDEAGSRVHIGSVSVPKEIEELEKRIEELRQQKMKVLSEQ
                     KYELAGPIRDQEKQCQYQLEDAIRRWEQESQQHPEIVDEEQVAEVVAMMSGIPVQRIA
                     QAEGLKLRQMKDVLQSKIIGQEEAVNKIVKAIQRNRIGLKDPNKPIGSFIFIGPTGVG
                     KTYLAKVLAEFMFDSPDALIRVDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRR
                     KPYSIVLLDEIEKAHPDVFNLLLQVMDEGRLTDSLGRRIDFKNTILIMTSNVGTRQLK
                     DFGKGVGFNTIETPEMDAEFSQGVIQKALSKTFAPEFLNRIDEVIVFNPLSKDAIKSI
                     IDLELKGLFGRITSMGYRLELTPEAKDFIAEKGYDAQFGARPLKRAIQRYLEDELADI
                     VLSGDLETGDTILMDLDAENGKMKAKIVKPAPVLNQNNN"
     gene            15811..17007
                     /locus_tag="PLM04_05665"
                     /pseudo
     CDS             15811..17007
                     /locus_tag="PLM04_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17633..18244
                     /locus_tag="PLM04_05670"
     CDS             17633..18244
                     /locus_tag="PLM04_05670"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HON02597.1"
                     /translation="MRNLWQQLRTEGIEDERVLKAMANVPRELFVGNSCVEEAYENTP
                     LPIGHGQTISQPFIVAYMTECLHVEPDDFVLEIGTGSGYQAAVLAQLAKKVYSVEIIK
                     ELGISAKERLKKLHYDNVEIIIGDGYYGWLEHAPYDKIIVTAAANEIPPPLVEQLKNQ
                     GRMVIPVGFPYTTQHLMLITKSGGKIASTTLLPVSFVPFTGKH"
BASE COUNT         5661 a         3388 c         3315 g         6199 t
ORIGIN      
        1 gcttgtttcg ttgtgattta ttttccgagg aaaatatctc tcttctcttc gttttaaaat
       61 cacaaaaatg taatttcagg ttttgagtga taagaatcga ctttcaaaaa acgtaagaca
      121 aaatcaatgt ccttcagata ggttttccat acctttttcc tgagaaacgg caaattattt
      181 ttgcaggtat ccagacagaa ttgtaccaat tcagctgagt aaaaattaga taataataac
      241 tgccaaaaat cggtttcact tttctccgga tgaaagaagg gattgctttt catgggaaat
      301 gcgctgttaa tgccaacagc gatttctatt ccagaatggg gattaaaaaa aactatacag
      361 ggatcatcaa gttcatcggc acttatttcg tcttctttct ccaatttcaa atcttttttt
      421 tgtttagcat tttttttaaa tctttctttt tcgtcttccg aaatatttaa tttgtcattg
      481 taacaattgg ttactttttc aataaattcg ttcacttggc ttcttttcat aaatgcaata
      541 aaatttccat tgtttgcatt caaaaaagcc atcatttgat tctttattaa cttatttcct
      601 tcaaattctt caaaatcaag aaagttgaca gtttgcttga atgcaagagt atgttttaca
      661 tcttcaatca atttcttatt gtagtccatc ttggaagaag tacctgaaaa ccaccattct
      721 ccacgccact tcacaattcc aataagcaaa attgtatcta cttttttcaa atcgttgtaa
      781 tgttcataag atttttttgt catttcgaat tttcttcctg aagcaatatg ttcaatgaaa
      841 atattgttct tgtcctgacc tttataaaga aaaaaacctt taacttttgg agaaatattt
      901 ataacatctt tatataatgg atgtttttcc cccaacaatt cggctgccca ctcttttccg
      961 gtcattgcca acaggctggt attagaagtg tttataaaat tatcctttat ttcataaaat
     1021 aacattgcct ctctatcctt gtcaacagat ttttgcttgt tcttttgctc tttttcattt
     1081 tcttttttaa acctgatata agtatcagga taaaacaaat aagtattaaa caatatcaat
     1141 tcgataagac ctcgaacgga aaaataattt gtttcatccc tgtttagttg gtaatatgat
     1201 tttaatattg gattttctgg tgcatattta tattcatcag ataagatatt ttttacttca
     1261 aaagccatgt ccataatttc ttcattgatc gggctgataa aaaagttttg ttgaatggtg
     1321 ttcaaaaagt accaaatcag aaaattaaca tcctgcatat tcacattatt cagaatgtaa
     1381 ttttccaacg gaaaaaaagg cagatatttt ttatacaaac taaaatgttt ttttgtaaag
     1441 gtattccaga tattgcttcc agtaacaata tcttccaaat aagaaaccaa aaaacagcaa
     1501 agaatatcta tttcatcttc atttaaatat tcgtttagga aagctgaagg ttgttttttt
     1561 gtcaacacat tttttacctt gttagccagc tcaacataat atttatccgt tgatgtttgt
     1621 tggctgtatg gcttgagttc cagccactgt ttgagattga ttctgtcggt tgattttgta
     1681 ctcatttgct gacctaactt tttattttat aatcaaaatt ttgatagaat tttctactga
     1741 ttgcgttatg aagacttgca cgattttttt atacaaatgt aaaaaaattc actgactttc
     1801 aaatgttaaa ttgtttcaac aaaaacctct atcctaaaat ggttttcgat gatagaattt
     1861 ttgaagcagg aatttaattg tagtgataca aaattgtcat gcaccatttc tacttttgtg
     1921 ctgtggaaaa gagttttgaa aagagaagtt ttaaaaatct attttcaaaa aagatgaacg
     1981 gtttctttat ccctttagcc attagcaata tctgaacaat aataaaacct ataaaaaaac
     2041 atgtattcca ataaaaaaat taaagtagcc ttttttgccg atattcttac tcgggatcat
     2101 gatggtgcca taaaaacaat gtatcaacta atagataagg tacctgaaga aaattttgag
     2161 ttcatgtttt gcaccggcac tcctccaaaa gaaaaaataa accataaaat actacaaatc
     2221 ccttcattaa cactaccttt taattcaaat tacaaagcat gtctccctca ttttagcagc
     2281 aataagattg ttaaagcact tgaagatttt gatccggatg tgattcatat cgctactcca
     2341 tcgccacttg gtttctttgg actaaatttt gcccgaaaaa aacatattcc tgtcttgtca
     2401 atttatcata ccaatttcct ttcctacata cagtattatt tcaatcgttt gccattttta
     2461 ataaagccgg tagaatcgat tatgaaaaaa atttatcaaa gcttttacaa tttttgtaat
     2521 atggtttatg ttcctacaaa gcaaatgatt atagaactga aggacagtgg tgtttcgcat
     2581 caacctttga agctttggca aagaggaata gattctaatt tgtttaatcc ttccaaaaaa
     2641 gacatgcatt acattcaaca aattacaggc aacaataaac catgtatttt gtttgcatct
     2701 cgtttagttt gggaaaaaaa tttagagaca ctttttaata tttatgatga aactgaggct
     2761 caaaatttag acgttaattt tattatagct ggaagtggtg ttgctgagca ggcagcacga
     2821 aaaagaatga aaaaagcttt ttttcttggg tttttaaatc acgaagaact gtcgaaagtt
     2881 tatgcatctt ccgatgtgtt tgttttccct tctatttcgg aaacatttgg taatgtggtt
     2941 gtagaagcaa tggcttccgg ttgcgtaccg gtaattgctc gtggaggcgg ttcgcaatct
     3001 ctggttatga atggagtaac cggatttttg tgtaatcctt atgatgctcg cgaatatgta
     3061 gagaatatta aagttattct aaattatcct tccttgaggg aatcaataca gcaaaatgct
     3121 attcgttttg catccaattt taattggaat cgcttggcaa atgaatattt ctccgatctt
     3181 caaatgttgg cttataagtc atcgcctact tttgaagaca atgtagtttt aacaaatgaa
     3241 cttttcccta ccaattatgc tatctgatta aaaaactctt agacctcaga catttattca
     3301 ctaagctaac agaatgagca aacttaaaag atatcgaaaa ctgattatct cagttttgat
     3361 gatttcggta tctgtgctga taattcgatt tatatcgagc attgacttcc atattctgaa
     3421 tatgtatctc caacgaatgc cctccatgtt tttattggtt attgctattt catttgttgg
     3481 gtattttttt tcttcattgg gttggaaatt gtgcatgggt gaggaaggaa tgaatatttc
     3541 tatttggaag atatttatta tcaggcatat aggagaaatg ttgggggctt ttaatcctac
     3601 cagtattatt gcaggcgaca gcttaaaaat ttcttatctc gtaaagtccg gaatttctac
     3661 aaaaattggt ttgagttcca tcttgttgat gagaatgatt actgtgttgt catccatttt
     3721 tctgatcgtc gtttctatat tttatttgat tttcagtaaa tttggcaatg ggaaaacttt
     3781 tgtacttttt ttgttaattg cgttggtaat gatttcgggc tttttaatgg caaaatattt
     3841 actcgatgat catttatatc ttggtaaaac attggagtgc attaaatcaa aaaccaactg
     3901 gtcgttcttg acgagcaaaa caatcgattc gtgttatgaa ataaatacac ttgcttcaaa
     3961 ttatttcaag caccacaaac tgaacttctt attggctttt ttattattga cacttcattg
     4021 ggtatgtgga gcccttgaat tttttatcat actgcaaacc ttacatgtga atatttcggt
     4081 aatcgatgcc atctcaatgg aagttggtgt ttcgcttttt aaatctttgg gtgcaattgt
     4141 tcccggccag attggaatcg aagaatatgg gaacaaaatc atgcttgatg tagtggctgt
     4201 aaaaggaaat gaaatttggt ttgtggtttc tctggtgaga agatcgcgtc aacttttctg
     4261 gttgtctgtt gcaggagtat ttttctggat cacaaaaagg aaaaaattta cacttcaata
     4321 atgtacgagg agatattatg cattacccat aagtatccac cttttaccgg tggtatggaa
     4381 aaacagagtt atgaactgat tcagggttta tcctcctatt acaaagtaca cattattgct
     4441 tttgaaggaa aggaaaataa attgctttgg tttttgaaat tgaagtcgag agtaaaaaaa
     4501 ttattgaaag caaatccaaa cataaaactg attcatttga atgatggagc tatgggagtt
     4561 gcagcacttt ggctacagaa aatcactcaa attcctgttg ttgtaactta tcatggtctg
     4621 gacataacct atcctttgac tttttttcaa aaaaaactta tcccaaaact aataaaatac
     4681 aaaggtactg tttgcgtaag taaatttacc agcgaacagt gtatcaaaag gggttttgac
     4741 ccgaaaacca cctttatcgt aaacaatggg gtagatttga agttgggtga aattcctttt
     4801 gatccagcca ttgctcagaa acttgtggaa aaatacggcg tagatgtaac cgataaaaaa
     4861 attatcatgg caaccggcag accggtgaaa cgaaaaggtt tttcatggtt tttaaaaaat
     4921 gtcatgcctt tattggataa tgatgtgatc ttgattttaa caggtccact taaaaaatct
     4981 caaacatttt ttgacatttt cttacggtgc tttccaaaaa tgctacgaca taaaattcag
     5041 ttgttatttg gttttcctga tgataataaa gaagttatag aacaattgaa aaaacaaaga
     5101 aatgcctttc atctgggaag agtttcaaat gaagatctta tgcagataat ttcgcttgcc
     5161 gatttatttg taatgcctaa cattaatatt ccaggcgatg tagaaggatt tggcttggtt
     5221 gcactcgaag ccagcgttcg gggaacgtat gtgttggcat ccaaagtaca aggaattaca
     5281 gatgcagtta tagacatgga aaatgggacg ctacttcctt gcgaaaatgc tgaagcttgg
     5341 gcagataaaa taaaagaaat gctgagcgac aaggaaaaac ttaaaatatt gtcggagaaa
     5401 ggcagaaatt ttacaattca acatttttca tggcaaaaaa tggtcgatga gtacatacaa
     5461 gtattcaatg gctttatcca ttaaaagata ttgtatttta tccaagttgc aacttttttg
     5521 gcttactgta ataaaccaaa attactcatt aaccaattta atgaaaaagt cttactccaa
     5581 gaactgttta taagtgagga aaatcacgtt tgtcttcttg tatttgaaac aaaatttaat
     5641 aacggtttgt tgttgatttt ttctaaatat acagtaacaa aagtttaaat tttcagtgta
     5701 agagtccact tttaaaagtc tgttttttta atttcttatt aaggcttagc gttgatattc
     5761 tgtgttttac aaatatcaaa gttgtcaaat ttgacttttt ggagtggact cgcgtaataa
     5821 aactttctgc gtttttttac aatatgagca tactgtgaac aacaacataa cataatgcac
     5881 tttcatcaaa aaaaataacc ccgaggcaag cctcgaggaa atcttttgat taaaatccga
     5941 ataggtaaag gttatatcca ttttacataa cagaaatcct gacgatgagg caaatcttca
     6001 tttttgggga acatccggaa tccgaattgg aacaagcctg ctctattgag ctgatattcg
     6061 agactgaaat acaatagtga accattcgct ttcaccagct taaactcttc cacatcatga
     6121 agatagaatt tattgtcctt atcgggtttg gtaatgacca gttctattcc gatacccttg
     6181 tcattaatgt ctttgatatc aacgacaata ttcatgtgat aaacctcact aacttgagga
     6241 tttttcaaca aaccttcagg aatatccatg ctcacaactt caattttgtc ccaccctttg
     6301 gcaattttct gtttccatgc agcaatttct ttggcacgcg caaaattatt atcttttaat
     6361 aatgcatgac gattgcttag tttggtgtaa aactgctcta cataattgtc aatcatgcgc
     6421 ttggttgtaa aacgtggtgt aatttgtgca atggaatctt tgatgtattg aatccattcg
     6481 ggcgaaattc ctctggaatt tttggcaaaa tacaatggaa taatttcatt ttcgagcatg
     6541 ctgtagatgg ttgcagcatc aagctgatcc tgatacgcat cgttatcgta agttcgtttt
     6601 tcggtaatag cccaaccggc tcctttcacg taaccttcca accaccatcc gtcgagaacc
     6661 gaaaaattca atacgccatt catttgtgcc ttttgtccag aagttcccga agcttccaaa
     6721 ggacgcgtag gggtattcaa ccaaatatcg acgccggaaa tcaatcgttt ggccaaatgc
     6781 atatcataat tttcgaggaa aatgattttg cctaaaaatt cgggcattct agaaacctcc
     6841 actatgcgtt taataagcgc ttttcctccc tgatcggcag gatgtgcctt accggaaaaa
     6901 ataaactgca caggttttga cgggtcattt acaattttcg ataaacgctt caaatcggta
     6961 aataaaaggt aagcgcgctt gtaagttgca aatcttctac caaaaccgat gatcaaagca
     7021 ttgggattca tcgattccag aaccgacatg atacgggaag ggtcgccctg tgacttcagc
     7081 cagttctcct gaaattgcaa acgaatataa tcgatcaatt tgtttttcat tttgacgcgc
     7141 gtctcccaga taacttgatc gggcacttcg taaatagcct gccagagttt catgttcgac
     7201 tgatcgtcat aaaaatcttt tccgagatat ttttcgtaaa ctcctttcca ctctgaagtg
     7261 gtccatgtgg gtaaatgaac accattggta acatagctca catgcagctc ttcaggataa
     7321 taacccttcc atattggctg aaacatttct tgtgaaacct tgccatgcaa ccaacttaca
     7381 ccattaactt cctgacaggt attgcaggca aaaacactca tactgaattt gtcattggga
     7441 tcatctggat tttcacgtcc caagttcatc aagtcgttcc acgaaatatt cagcaaggca
     7501 gcataatctt tcatgtattt tccaaacaag gcttcatcaa agcggtcgtg tcctgcagga
     7561 acaggtgtat gtacagtata aagggaactg ctccgcacaa tttccaaagc ctcgtggaaa
     7621 gaaagttttt tctcggttac taaatccgcc agacgctgaa gattgataag ggctgcgtgg
     7681 ccttcattgc aatgatagac atccttttcg atgccgagtt ttttcagtgt cagaattcca
     7741 ccaattccca gcatgatttc ctgtttcaga cgattttccc aatcgccgcc atacaactcg
     7801 aaagtaatac ttctgtcagc ttcactgttc atttcattgt cggtatccaa aaggaaaaga
     7861 ttaatacgtc caacgcttac tttccacaaa ttggcataca cggtgcgtcc cgggtaggga
     7921 acttctataa caaccgcttg tccattttca tcacgaacct gcacaatagg caaagtattg
     7981 aagttttgtt tgtcataaat ctcaatttga tcaccgtcga ccgaaagcga ctgagtaaaa
     8041 tatccgtaac gataaagtaa tccgacagcc gtcaaatcaa cgccacaatc actggcttcc
     8101 ttcaaataat cgcctgccaa aattccaagc cctccggaat agatttttaa aacttccgtc
     8161 agcccgtatt ccatactgaa gtaggcaatc gaaggtttat cgggtgcaaa aggttgatgc
     8221 agataagcct gaaattcgtt gtaaatcctg tctaagttgg acatgaaatg ggcatccttc
     8281 tccacttcta caagtcgctc ataactgagc atgttcagca attgcacagg attatagccc
     8341 gattccgccc aagcatcttt gtccaattca cgaaaaaggt gtcgggcatc acttctccat
     8401 acccaaaata tattttgcgc tatttcttcc aattttttca agccatcggg caaaaaagac
     8461 ttgacattta tttctttcca gcgcggttca tttacctgat ttaatttcaa agacatgttt
     8521 ccttacttta agttagtgaa attcaaaaaa aattttatac ctaaaactct gtaggcaaag
     8581 ttaagcaaag tttcaaaaag ccaagataaa acaaatcata aaaggagtaa aaacaaatgc
     8641 aaacgattgc aatttattaa aaaatttttg ttaatatatt ctttttcaat taattaatta
     8701 ttaattcgtt acaaagaaaa accgaaaaaa tcgatttttt tcttaattct aatgtttgcc
     8761 aagtcggaga tttttttgag taagggctat attgtatgct tcataataat attgtatgaa
     8821 gttttcccat aaggctttca gggaaattga agccgctatt gagcgtatat tttctacttg
     8881 agattgctcc aaactcgaaa actgacaaat catttgtgca attttcacag ttgcatcata
     8941 ctgattgtag tcggaacgat gtactactcc cacaccttcg gtaatatcct gaggttgttt
     9001 tgccacccag cggccaaaac ccgacaaatc gctggtaata gttggtacgg aaaaagcaat
     9061 gctttcgaga ggtgtatagc cccatggttc ataataggaa ggaaatatgg taacatcgag
     9121 cccaatgagc aggtcgtaat attttttatt gaatatgccg tcggaaccat tcaaataaga
     9181 gggcacaaaa atcatcttaa ccttttgcga cgattgattg gtaaaatgga accaccgaat
     9241 agccgacatg atatgatcat tctgataatc gtaaagttcg tgcgtggtaa acaaattcca
     9301 tgaatgcaac ttgagatttg gatgatttaa agctgcaagt aaatccgttc tggggcccat
     9361 cacatgggcg ggcaccataa tgaaaaccac aatttctctg ttcaaatgag ggcattcaat
     9421 caacctccgc atcgattcga taaacacatc aatacctttg ttcttgtatt cgtaacgtcc
     9481 ggcagtaccc acaaaaagag cattttcctg taaagaataa cccaacaaag tttcagcgac
     9541 tttgcgtaaa caagttcgtg cttcctgtcg cttttgaata aaagtatcgg ctttgggcac
     9601 aaaatcatct tcaaaaccgt ttggtgtaac aacagcgggt ttaatttcca gcaattgtcg
     9661 gcattcagcg gcagtaatgt cgcttacagt tgtgaagcaa tcggaaagat gagcggcagt
     9721 tttttcggcc gaatgtttgg aaacaacatt caattcggca gccatttggt ccccattgta
     9781 ctcttgcaga taatcataca gcggtttgtg attgctggct atggaacgtc ccacagtagt
     9841 ggcatgagta gtaaaaatgg ttgcagcttc aggcaaatgt tcttttaagt aaaaaatacc
     9901 gaaagttgtt tgccactcat gaaaatgggc aatcacatca tttttcaaag acaaaccaaa
     9961 aaatcgataa aaactttcta tcaccatacc tgcagcatag ccaaacatcg aagattcgtg
    10021 ataatcgcca taggcaggca aggaattgac tccggtttgc agccaaacat ggccataaat
    10081 ttcatctttt cgtagcatca gcggcatgaa atcgaccaat actgcaatcg gatttccatg
    10141 aatttgccaa cgccctatac gaatattcaa ctgaaactgt tgctgaacag catctttcca
    10201 accggggaaa agcctactgt cttcaacaaa aagcgggttg tttttttgta tccatatatc
    10261 aggtccaata aaaatcagtt tgtctccata aacttctttc aatgttgcag cacgagtaga
    10321 taaaacggta tatattccac caaccatatt gcatacttcc cagcttgttt caaatatgta
    10381 ttccggcttc aacatactta atttggtata atcgaaatta aactttctgt tgttttttga
    10441 aaacggggaa aaattgaaaa taaaactgat aaaaaattaa ttatctatca atgattttaa
    10501 aatcaaacaa aagtgataaa aaagtatttt tgaaaaaact aaatatttgt tttatgaata
    10561 tactatacct tgcttgtcaa aaaaaatcct acttacgtag gatatacttt ttgaacagcc
    10621 aaattagaaa ttattttttc ttgtttgaaa atcgttcgtt caacagcttc accacttcat
    10681 cggtaatatc gtaacttttg gaagcaaata ggatattgtc gcctgtggta ttactgaaaa
    10741 taatttcata acggccatcc ttattatatt ctttgagaaa ggcattaatg gtatcgcgca
    10801 attcttcact cattttttgt tgttcctgta aaagctgttg agtaagttgc ccatcaagat
    10861 tttgtaattc ttgttgtttt tttaataaac gcgtatgttc ctgctctgcc cgctcccggc
    10921 tgagaaatgc attgttttcg agttttctct gaaattcgtt catttcattt tgcagttcac
    10981 gagcacgtga gtttatggtc aaccgtgaat cttcctgttt tttaatgagg gattcattgg
    11041 catcctttgc aaaccggtat tttaataaca aggaatctac atttatgtaa gcaataggta
    11101 aaacctgact tgacagggta tctcctttta atgacaactg aaccgcattc ttagcgggtt
    11161 taatctgaaa aaacaaaaca aaaagtacaa caacagcaat gaaaagaacg atgttggaaa
    11221 caaataacaa atttttcata tttaagcttt aaaatattat ctttaattca taccatgcaa
    11281 agacaaaaat aaaattcaaa aagacaatct gcatgaaaca acctattcgt tttttaattg
    11341 ttcagaaatt tcagttaaca aattggaaac atcaatccct ttatgaaaat tccgttgggc
    11401 ttctttgtcc tgtgtggtag cacagccaat acctctttgt tggagggctt tgcttccacc
    11461 aatatgaata ttgggaaatt ttccactttt aatgaagaag atacgaaatc ccaataataa
    11521 aaccgaaagt aaaacaatca gtaaggttat aagtaaggtg taaaacataa acttaaaaat
    11581 ataacggctt gcaaagataa gatatttaat agaaaatgaa attatttcta tgatttttta
    11641 gcaaaacaaa taatttattt gctttattag taattcttaa atatttatgc aataaattaa
    11701 aaaccggcct atgttataag ccggtaaaaa aacaaaaagg taaaaaacaa aataatcaga
    11761 aacgttccga cactatgctc caatttacca gatcccataa agcagctaca taatccgctc
    11821 tccggttttg gtaatcgaga taataagcat gttcccaaac atcgaaagta agaatgggag
    11881 acagattttt agtcatcgga ttccccgcat tactttcgtt gacaatggaa agctttcccg
    11941 aagcatcctt tacgagccaa gcccagcctg aaccaaacaa tccggtcgca gctttgccaa
    12001 attcaatttt gaaattatcc aacgatttcc attcggcttc gatggctttc agtagttttt
    12061 cgctgggttt agtaccttta tcaggggtca aggaaaggaa atagaaattg tgattccata
    12121 tttgtgcggc attgttgaaa agcggacctg tcgctttttt aacaatggtt tccaaatcct
    12181 gattttcaaa ttctgtacct actaccaaat tgttggtgtt gtttacgtaa gttagatgat
    12241 gcttgccgta gtgaaaactg attgttcttt cactgataac cggttccaaa gcattctgtg
    12301 cataaggtaa ttcaggcaat tgaaatttca taacaataaa attttatggt taatactaaa
    12361 ttgattttat agaaaattta ttgttataac aatcaatttt ctgttttgtt ttaatattta
    12421 cccaaaaaaa aatcaacgga tattatctct tacttttcaa aatttctata gttctgttaa
    12481 aaaaaaaaat ccaaataatt atctttcaaa ttaataacta taccaatttt tttaagtaaa
    12541 gaattttttt gaccaatgga atttgaagtc gataaaggga aacgattcaa tatgtttata
    12601 tacaaagaaa gccctgaaat acaggacttt tcagcggaga gagagggatt cgaacccccg
    12661 gaacctttca gttcaacggt tttcaagacc gccgcaatcg accactctgc catctctcca
    12721 acatttctca aaatgcggtg caaatatact tctttttctt ttatcctaca acaatagttg
    12781 cggttttaaa gagatatttg gcacgatttt cattcaaaac agacaaaaaa aatacctgcc
    12841 attgcatcga atagcaggta catttttgat ttttgcttga tattttagtt gttgttttgg
    12901 ttcagtaccg gagcaggttt aacaattttt gctttcattt tgccattttc ggcatccaaa
    12961 tccattaaaa ttgtatcgcc cgtttccaaa tctcccgaca gcacaatgtc tgccagttca
    13021 tcttcaaggt atcgttgaat ggcacgtttc aggggtcgtg caccaaattg agcatcgtaa
    13081 cctttttcgg caatgaaatc tttggcttcg ggtgtcaatt cgagtcgata acccatagaa
    13141 gtaattcgtc caaaaagtcc ttttaattca aggtcaataa tagatttaat ggcatccttg
    13201 ctcaacggat tgaatacgat tacttcgtct atacggttca gaaattcggg agcaaatgtt
    13261 ttgctcagcg ctttctgaat tacaccttgt gaaaattctg catccatttc aggtgtttca
    13321 atggtattga atcctacgcc tttaccaaaa tctttcagtt gtcgcgtacc gacattggaa
    13381 gtcataatca gaatggtatt tttaaaatct attctgcgac ccaagctgtc ggtcaatctt
    13441 ccttcatcca ttacctgaag gagaaggttg aaaacgtcgg gatgcgcttt ttcaatttca
    13501 tccagcaaga ctattgaata aggtttgcgt cgaactttct cggtcagttg accgccttct
    13561 tcgtaaccca catatcccgg aggtgctccg ataagtcgcg atacgctgaa tttttccatg
    13621 tattcgctca tatcgacgcg aatcagtgca tcgggactgt cgaacataaa ttcggcgagc
    13681 actttggcca gataagtttt tcctacgcct gtggggccga taaatatgaa agaaccaatc
    13741 ggtttatttg gatccttcaa tccaatacgg tttctttgaa tggcttttac aattttgttc
    13801 acagcttctt cctgcccgat aattttgctc tgaagtacat ccttcatttg tcggagtttc
    13861 agtccttctg cttgagcaat acgctgcacg ggaatacccg acatcatggc aactacttcg
    13921 gcaacctgtt cttcgtctac aatttcggga tgctgttgtg attcctgttc ccatcgtctg
    13981 atcgcatctt ccagctggta ttggcattgt ttttcctgat cgcgtattgg accggccaat
    14041 tcatatttct gttccgaaag aactttcatt ttttgctgac gaagttcttc tatacgtttt
    14101 tctaattctt caatttcttt cggtacggaa actgagccta tatgaacgcg cgaaccggct
    14161 tcgtccaatg catcaatagc tttgtcgggg aagcatcgat ctgttatgta acgttcggta
    14221 agtcttacgc aagcttcaat ggctgccggc gtataacgca cgttgtgatg gtattcgtat
    14281 ttatcctgaa tgttgcgtaa aatctgcaat gtttcttcgt aagaagtcgg ttctacaata
    14341 attttctgga atcgacgttc caacgctcca tctttttcga tgctttggcg gtattcgtcc
    14401 aaagtggtgg caccgataca ttgaatttca ccacgtgcca atgcgggttt aagcatgttg
    14461 gcagcatcca gtgaaccggg tgcgccaccg gcaccaacaa tggtgtgaat ttcatcaata
    14521 aaaagaatga tgttcggatt ttttgccaat tcgttgagaa tagctttaat gcgctcttca
    14581 aactgtccgc gatattttgt ccctgccact atggaagcca tatccaatgc aacaattcgt
    14641 ttgtcgaaga gtacacgcga aactttatgt tgaacgatgc gcaaagccaa accttctact
    14701 atagccgatt taccaactcc gggatcacct atcaatacgg gattgttttt ctttctacgg
    14761 ctaagaatct gagccaatct ttcaatttcg acttgtctgc ctactacggg atccaacctg
    14821 ttttcgagag cagctttggt aatatctgtt ccgaatgtgt ccaaagccgg agtatcactc
    14881 tgtttgccac ttcccgaaac atttcgctga tgttggcgag gttcatcatc ttcgtcatca
    14941 tcatcctgaa aattggaact catttgaata tccttagaat gttcaagata cgatttggca
    15001 atttcatagg tcagattttc ttcatttaaa acttctacag ccaaattgtt cttttctttc
    15061 agaatggcca aaagcaaatg ttcagaatcg gctgtttcgg atgaaagaga acgagtttca
    15121 agctccatga tttttttaat gcgttcggtg cttttcaaac ttaaaatttc ttctccggta
    15181 ggttcgttat ctgtacgaat ctgactttcg attgactgtt ttatttttga caagtccact
    15241 tgcgtgtggt tgagaatgtc gatagctttc cctttgccgt ttctgattat ccccagcagc
    15301 aaatgttcag gccctatata attgtttccc aacctttcag cttcctgaag gctgtaaaga
    15361 agtatgttgt gtaactgttc tgaatagttg acggtcatat ttagatttct ccttttttct
    15421 acaaaaataa ttaatttaat gtgatgttgg acactcaaag tttgacttta gaaattttaa
    15481 ttttttataa gaaaacgaat ggattaaaat tttttcatgt caacattagt aaaactttaa
    15541 tcttgaaatc gtttactgtg cttattatta tcaaactttt cactccaaaa ttttatttta
    15601 tctatcaaaa ataataccga tttgttttta tcgtgtcttc tgaaattttg tcagtataaa
    15661 gtgaactgtt gtcaggctgc aaaaaaatat gaataggaat gcctgcatga aattgttttc
    15721 aaaaattttt tcagtagttt tgcatttctt tatttttaaa taccaagaac ataaattcaa
    15781 taaaaaaaca accattaaag aaaattgttt atgtcgcaga taaaatcagt ttcattacgt
    15841 gaatcaccgg tggcacgttg gacggctttg ggaatagttt cctttaccat gctttgcgga
    15901 tatttcattg ccgatgccat gagtcctctt aaaccattgc tggagagtca gttgctatgg
    15961 agtagcattg attattcgct gtttaccagt gcttatggat ggttcaatgt atttctgttg
    16021 atgcttattt ttggtggtat cattttggac aaaatgggtg ttcgttttac aggattggcc
    16081 tctacagccg tcatggtgat tggggcagcc atcaagtatt ttgccatatc gccttttttc
    16141 cccgatgatg ccgggactat tctgggcatc aaggctcagg taatgatagc ggcattggga
    16201 tatgccacat ttgccgtcgg agtagaaatt gccggcatta cggtttctcg tgtcattgtc
    16261 aaatggttta aaggaaaaga gatggcgctg gctatgggac ttcaaatggc tacagctcga
    16321 cttggaactt tgctggccgt cgctattccg ctccctgttg ccaaacattt taatgatgtt
    16381 tcggttccta tcctgtttgg cgttttactg ctctgtattg gacttatcgc ttatattgtt
    16441 tacgttcaga tggacaaaaa gctggaagct tcagaagcaa atgatgaaaa ggaagaggaa
    16501 gaaccgttta aagtttcgga agttttcact attattaaaa ataaaggttt ttggcttatt
    16561 gccattttgt gtgttctgtt ttattccgca gtttttccgt ggataaaatt tgccaccgat
    16621 atggttatca acaagtatca ggttaatccg gatttggcag ggcttatccc gagcgttctt
    16681 cctttgggaa cattgtttct tactccatta tttggaaata tttacgaccg aaagggcaaa
    16741 ggtgcttcca ttatgattct tggcgcttta ttgcttatat ttgttcatgc aattttttct
    16801 attccatttc tcaataattg ggtcttggct attggcgtaa cgatagtttt gggaattgcc
    16861 ttatcgcttg taccttcggc catgtggcca tcagttccga aaattattcc ggaaaggcag
    16921 ttgggaacag cttacgcatt gattttctgg atacaaaatt ggggattaat gggtgttcca
    16981 ttgttgatcg gattggtgct gaacagtgca aatcctacgg tttacaaagc gaaagaaatg
    17041 gtgaagacgg gtgtggtgca aacttatgaa ttatatcaac cttcattggg actgaataaa
    17101 attgaatttg agaaagccgt tgataaaact actacggctt taatcaatga agtgttgttg
    17161 aaaagcgatt atgaaggtgt gcaggatacc gtaggttttg cagcctttaa aaatgaatgg
    17221 atacataaaa atattaccgc tttgccattg gaaaaactaa attcttttca agacaaaaat
    17281 cagaaacttc gagcggtaga aaatacttgc ttagagattg gttattcgga aatcgtaaaa
    17341 aataaaattg ctttgaggta cgactataca cgtccaagtc tggtatttat gatttttgga
    17401 gttctggcct tgctgtttgc tttcttgttg aaaaaggaag ataaagcgaa aaactatggt
    17461 ttggagctac ccaatattca aaaggaataa atttttcggt atttttattt aaagaaaagg
    17521 cggtttgaaa attttcaggc tgcttttttc tttcatgagc aaacaaaaat ttacaagaat
    17581 ttgtttttat aattgataat aaacaaatta tttttgtagt ttttattttg acatgagaaa
    17641 tctttggcaa caactcagaa cggaaggaat tgaagacgaa cgtgtgctga aagcaatggc
    17701 gaacgtacca cgcgaattgt ttgtaggtaa ttcttgtgtg gaagaagctt atgaaaatac
    17761 gccgttaccc attggacacg ggcaaactat ttctcagcct tttattgtag cttacatgac
    17821 cgaatgtttg catgttgaac cggatgattt tgttcttgaa atcggaacgg gttccggtta
    17881 tcaggcggca gtacttgctc aattggcaaa aaaagtttat tcggtagaaa ttataaaaga
    17941 attgggaatt tctgccaaag aacgactaaa aaaattacat tacgacaatg tagaaataat
    18001 aattggagat ggctattacg gttggctgga acatgctcct tacgataaaa ttatagttac
    18061 tgccgctgca aacgaaattc cgccaccact tgtagaacaa cttaaaaatc agggacgaat
    18121 ggtaattccg gttggttttc cctatactac acaacatttg atgctgatta caaaaagtgg
    18181 cggaaaaatt gcttccacca ctctgttgcc tgtgagtttt gttcccttta cgggaaaaca
    18241 ttgaagagaa gagtaatgtt ttacgttgcg ccaaatctac agtccattgc ttgtttctat
    18301 tttcgtaatt tcgctgccgt tcaatttaag caaccaattg gcctctatca ttaactgatg
    18361 ccatattttt ttcaatactt agttgcatca ttgaccggat tttgtatctt tgttctaagg
    18421 ttcatgtttt ttatgaaaaa aattgtttga attgtttgaa tttaggaaat atttttctaa
    18481 aatctgtatt aattcaccaa attattattt tgttattttt aaacaaattc tgtgatttat
    18541 aatttttgct ttattttatg aaa
//
LOCUS       DAOTAX010000042        18337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2523_length_18337_cov_30.494640, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000042 DAOTAX010000000
VERSION     DAOTAX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18337
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_18337_cov_30.494640"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..3597
                     /locus_tag="PLM04_05675"
     CDS             349..3597
                     /locus_tag="PLM04_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02598.1"
                     /translation="MTAYFNIYFNGLESYNEGLKNILVAHQDDYSTILPMFPISKHSN
                     ASAATANMDRAIEKSRKAIKLHSIKQKPEKNYNKWNSAEYQAFYNQEEFNPVLKDAWM
                     LIGKAEFHKGDFLGSAGTFSYIARHFASDPDLVAQAKIWLARAYGEMGWLYEAQQVLG
                     DVNQDHLKRQHIGLFASVNADILLKNHQYKEAIPFLRVAIDKETDKKLKQRFLFILAQ
                     LYQKTGDEKAAYETYTKVLQSNPPFEMDFYARIQRSELKAANHAEVQKDLNKMLKSSK
                     YKDYQDQIYYVLGKNFLHRGDTAQAINAFRSSIEKSTRNGYDKANSLITLGDLFYHQK
                     NYKEAYPCYDAASKIIAEEHPDYRRISKLADILSEFVTQYDIVMLQDSLQRLAAMPEK
                     QRMEVIQKIISQVEKEEKAAAKKAEEELLGLNYPNNDDEETLIPIGAQMGNSNDWYFY
                     NQNLMKVGKSEFQKKWGNRKLEDNWRRANKSASLFAEEPNAENVSSEMMMDSTSSTLA
                     ITDNKNPAFYLKQIPLTPLQLKISNEQMAQAMFNVGKIYYEKIQDIPSAIRTFEEFVG
                     RFPDHKLVPDAYFQLYLMESKLGNLANAEKWRNRLVGEFLQTDYSKMLSKPDYIQRLQ
                     RMYQEQDSIYNATYRAYSSNDFATVFRNFQYMQENYPLSELMPKFQFLYALSVGKTQE
                     SQNFEKELDLLVSKYPESDVSTMAKDILALMRQGMVAKSGTTHGSLLAKREEAEKLEI
                     SPSAQQQFSTDTKTKHRLLLITDAPREELNKLMYQIASFNFSRFLIKDFDLGIQPLDS
                     VRNILYVDNFDSYDEVVWYENTMASDAYLTELLSRLKVQELIISDENFALLKNGLSLA
                     DYMAFHDKFLGKNAGSASKQISALPEKEKKSESPKTIAATANKPNSVTETKKTVSTST
                     EKTPLVAPTQPAEQTPNVASVAEQPVKNEPVEKVEVKKEEEVPLFKNLFAYKPNDPHF
                     IAVYVVSGTVDVAKTKAAFDEYNRQNYDVLQLNVRFENVGKQQFFIIGSFTDANTAKS
                     YLLRMVKESKLFEGLKGASYRNLVGTQQNLNVMVQQNALNVYFEFMKQYYLN"
     gene            3629..5176
                     /locus_tag="PLM04_05680"
     CDS             3629..5176
                     /locus_tag="PLM04_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HON02599.1"
                     /translation="MRKERILWIDDEIEMLRPYLLFLDEKGYEVVTATNGNDAVEICQ
                     EENFDIIFLDENMPGLSGLETLALIKEIDPDVPMVMITKNEEENIMNMAIGNKIADYL
                     TKPVNPSQILMTLKKNIHRKEIVNEHATAAYRSEFGQIGMKINDSFTYDDWVEVYKKL
                     VFWELELSETESGMDELLRMQKMDANTTFSKFIKNNYERWFERPEERPLMSFEIFKNK
                     VFPLLDNGEKVFFVVVDNFRLDQWKMIRELLSDYYQFDEEDLYCSILPTATQYARNAI
                     FSGLMPLQIQQMFPQFWVEEEEEEGKNLMESELLQTQIQRYRKKYTFSYHKINDSVSC
                     QHLIDQLPHLENNNLNVCVLNFIDMLSHARTESKMVRELANDEKAYRSLTLSWFRHSA
                     TFDLFRAIAERGYKVVFTTDHGTIRVNNPIKVIGDKNTNVNLRYKVGKNLSYNKKEVF
                     EITQPAKIGLPSPNVSSAYIFACKDDFFAYPNNYNYYVSYYKNTFQHGGVSLEEMLVP
                     LIVMNPK"
     gene            5287..5976
                     /locus_tag="PLM04_05685"
     CDS             5287..5976
                     /locus_tag="PLM04_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HON02600.1"
                     /translation="MRIGLIGFGKTGKAVASVILQSESFELKWILRRKKLLDHRSAAE
                     VLGIETSSTEGLIYSTSNTNLENLMDTLPVDAIIDFSSGQGIYTYGELAAKRKIKIIS
                     AISHYKPKEVAFLHKLAQQTTVFWSPNITLGVNYLMISAKFLKKIAPWVDIEIVEEHF
                     KGKDGISGTALKIAEVLDMDKAKINSIRAGGIVGRHEIIFGFPYQTVRLIHESISREV
                     LVMEFCLLPKI"
     gene            6128..8167
                     /locus_tag="PLM04_05690"
     CDS             6128..8167
                     /locus_tag="PLM04_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HON02601.1"
                     /translation="MKKILFVLMTVAFLGCRSEKPATPAMETFPYTVDRFADIEILRY
                     RVPDFETLTLQQKELIYYLSEAALSGRDILYDQNNKYNLCVRRTLEAVYENYRGDRQS
                     EEFRNFITYLKQVWMGNGIHHHYSQDKFVPKFSAAFFAQAVKSIDAEVLPLEKGETPD
                     DLIRKLTPVIFDPTVFPKRTNQADGVDLLLTSAVNFYEGVTQAEAEEFYDRMRNPNDS
                     TPISYGLNSKLMKENGVLVEKTYKLGGMYGKAIEQIVYWLNKALSVAENDKQKDVIHS
                     LIKFYETGDLKTYDEYCIKWVQDTASLVDFVNGFTETYADPLGLKGSWESIVNFKNLA
                     STKRTETISENAQWFENNSPIDNRFKKENVKGITAKVVTAAILAGDCYPSTPIGINLP
                     NSDWIRHDYGSKSVTIENITEAYDRASLGNGFNEEFMWSDVERERAKKYGSLTNNLHT
                     DLHECLGHGSGKLLPGVDPNGLKAYASTIEEARADLFGLYYVADSKMLELGLLPDHEA
                     YKAEYYKYIMNGLLTQLTRVQTGKNIEEAHMRNRQLIARWVYEKGLPENVIELKKRDG
                     KTYVVVNDYEKLRILFGKLLAEIQRIKSTGDYNGAKTLVENYGVKVDPVIHKEILERY
                     EKLNLAPYRGFVNPVYKAIKNADGKIRDVKISYDENYVEQQLRYSRQYSNLPTFN"
     gene            8382..9539
                     /locus_tag="PLM04_05695"
     CDS             8382..9539
                     /locus_tag="PLM04_05695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HON02602.1"
                     /translation="MNTNNEKWHGSPKKGGNSYDSREGRHFEKGGNNSPFSKPGRNQK
                     VYSSRNTSNSNYDNRSTGKEPYYKNENKKPYRRPFTTDENSEGENRKLKLVKPGKFVE
                     TRFDDRQPNVTFRKRKNKTEGEFNPNDKYSLKKQLEYQKNNVDYSKPIRLNKFLANAG
                     ICSRREADEFIQSGVISVNGEVVTQLGAKIMMTDTVMFKNQPVQSERKVYLLLNKPKD
                     CLTTSDDPNNRKTVLDLVKNACSERIYPVGRLDRNTTGVLLLTNDGDLAAKLTHPKYN
                     KKKIYHAVLDKNLETVDYESIIQGVDLGDEHVSVDALEFVKAGNRREIGIEIHSGQNR
                     VVRRIFEKFGYKVVYLDRVYFAGLTKKNVPRGKYRFLTQREINMLKMGAYE"
     gene            9615..10181
                     /locus_tag="PLM04_05700"
     CDS             9615..10181
                     /locus_tag="PLM04_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02603.1"
                     /translation="MSEENLNNTRSDEQSPVSSQPTNKKGFFRTFISRHTGVFVLLCL
                     LIAVVIAAFIRIKLLERSFNKQQEALRVRYENKVDSLLQADRLTTAKVFSWAVRGELI
                     RDNRDQINQYFMNFVKESGVKKIKLIDPVSAKAIISTDKKDEGLVFADPTFFTEEIAV
                     RHLDSVTQIISPVMGLNKKIGILVIEYE"
     gene            10377..13604
                     /locus_tag="PLM04_05705"
     CDS             10377..13604
                     /locus_tag="PLM04_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HON02604.1"
                     /translation="MNDLMKRFKSLNVIFGWVAFAIATVTYLLTLEPTASFWDCGEFI
                     STAYKLDVGHPPGAPFFMLTARFFTLFTSDPTRVAVMVNTLSALCSGLTILFLFWTIT
                     HLARKVVIKSDEDYTTGNIIIILGAGLVGALAYAFSDTFWFSAVEAEVYAYSSLFTAV
                     VFWLILKWEEVADEPGSDRWLLLIAYLMGLSIGVHLLNLLAIPAIVLVYYFKKFTPSL
                     KGVLFALLGSFLILGVVLYGIIPGFVQVAGWFELLFVNKLGMAFNSGLYVYIVLSIAA
                     LTWAIYESYVNKNYLRIVLSFLVAITLVGIPFFGQHIVLGIILVAGLSFYFYKWKNKI
                     NVHWIHTSILMITLMLIGYSTYSVIVIRSAANPPMDQNSPDNVFSLRYYLNREQYGDR
                     PLIYGPVYNAPVKLRIDGNMCIPEQELGEAMYAPKPKKSPNEKDQYMLTGYKMDYKMD
                     ERFNMLFPRMYSTNSSHVQAYKIWGNVKGKPITFDYCGQQRTEMKPTFAENLRFFFDY
                     QLNFMYWRYFLWNFSGRQNDLQGNGEVDKGNWITGINFIDKYLVGDQTNLPTFLAENK
                     GRNAYFMLPLILGILGILWQLYGGKNGKQTFWVTFLLFFMTGIAIVVYLNQTPYQPRE
                     RDYAYAGSFYAFSIWIGLGVLEIFKFINKYIPKTPSAILASLLCLGVPVLMASENWDD
                     HDRSGRFTARDFGQNYLESCRPNAIIFTNGDNDTFPLWYNQEVEGKRTDIRVCNLSYL
                     QTDWYIDQMKREAYESAPLPISWEPEDYISGKNEVISVEDFIKQPIDVKTIMDFIRSS
                     DPQTKINGEAVIPTKQIYIPVDAQQVVKTGTVKPERAAEIVPKIDFNLKNRLTKSELM
                     VIEMLKENNWERPMYFAVTVGDDYYLGLKDHFELTGMAYQIVPVGAKDAGPGVNTDEM
                     YENVMHKFRFGGINNPKVYIDENIGRMCRTHRMFMGQLAGALLAEGDTVKAKAVLDYC
                     LEMIPGNSVRHDYTSVQIANYYYRLEEFEKGDAIMDSVAKDCVEYINWGFSLSPSKQN
                     SITGDLGNNMAVLSQILQICDELKRKNILDKYLPVYMQYSRYFQM"
     gene            13605..14213
                     /locus_tag="PLM04_05710"
     CDS             13605..14213
                     /locus_tag="PLM04_05710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HON02605.1"
                     /translation="MSCQFPAFLRPLIGRNYLWRKNSSEKVVYLTFDDGPVPEVTPQV
                     LGVLDDFDIKATFFCVGDNVKKYPELYREILLKGHSVGNHTFHHLNGFKVGQEQYLKD
                     VEQASQLIDSRLFRPPYGKLTRKEKKILEQKFQIVLWDVISYDYDQNLSSSQVLKNVK
                     RYTRSGSVILFHDSVKAKNNMLTVLPQAISFLLAKGYRFEKL"
     gene            14283..15560
                     /locus_tag="PLM04_05715"
     CDS             14283..15560
                     /locus_tag="PLM04_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HON02606.1"
                     /translation="MKESILQYVWQHKLFLQSNLQTADGEKVEIIDVGKSNTDAGPDF
                     FNSKIKIDGTLWVGNVEIHLRSSEWIKHGHQKDKAYDTVILHVVAEMDGEVFRTDGTK
                     IPQLVLPFSKDIEANYDSLLNNNKWIPCADKIEKISEIFIQSWKNALLVERLEQKMTE
                     IENLLTQNHYHWEEAFYIIIARNFGFGTNGQAFELLAKSVPLNVLAKHKDNLLQLEAI
                     LFGQAGLLPVDTSDDEYAMQLIKEYAFLRNKFSLQPIDCSQWKLFRLRPYNFPHVRIA
                     QFASLIHQSSKLFSKIIETPNLELMKDLFSCKPSNYWQTHYLFGSASPKSTKQLGLYS
                     IYSIVINTIVPFLFCYASKKNNEDLKDKSLHLLEAIPSEKNAIVKGWRNIGIASESAF
                     DSQALIQLKKQYCDTKKCLFCRIGHKVLTLQTT"
     gene            15573..17408
                     /locus_tag="PLM04_05720"
     CDS             15573..17408
                     /locus_tag="PLM04_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HON02607.1"
                     /translation="MRLNFFKTIAFSGIILSVVGCANKAQGPTGGPKDSIPPSVVRSV
                     PSNGALNFKKKQILIDFDENVSIEKAAENVLISPPQVKPPEVVAIGKRVTVNLQDDLK
                     DSTTYTINFGNAIVDLNEKNPLKNFSFSFSTGNQIDTLQVKGTVINAEDLNPVSGVIV
                     GVYNDLSDTVFTQKPFLRIGKTDEIGKFSVENLKKDTFKIYALGDANRDYFYQPGEGL
                     AFTDSLIVPGFFREEVMDTIWKDSVQIDTIRSIVRTHFFPDNILLKYFKENKKRQYFI
                     KAERTLPYAFSLFFSAPMTELPTLKPIDFEWDGKYILQKNNTMDSLTYWMTDSLVWKK
                     DTLQFEMSYQKTDSLFRLVPATDTLKIFMRTARSTVRNTKDKKEPVSRVKMEQNYQFT
                     TNIAGSFDIYQPVVFRFSTPLAYFDSSKISLAQRVDTILKPLPVNWRQVDSTKMVYAV
                     DYKWEPEKNYELTVDSAAFTSIYQQTSKKYSGKFKIRSLDEYSTVIMVLENFVPSAVI
                     QILDAKDAVIASKQAQEKGTVFQYLKPGDYYARLFLDENGNGKWDTGDFAAHRQPEAV
                     FYYPKKMTLMPNWEFEETWDYLKVPLLEQKPKEIRKDAGKKTSGN"
     gene            complement(17450..>18337)
                     /locus_tag="PLM04_05725"
     CDS             complement(17450..>18337)
                     /locus_tag="PLM04_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HON02608.1"
                     /translation="QTTLPTIDPTTKLTLANSLWYRSGFQVKPDFLKVNIDYFNAYVR
                     ELDFSKTWAVDTINQWCAKKTNNLIPSVLDAIPDEAVMYLINAIYFKGIWRHQFEKKN
                     TKQTDFTNEAGEKVKVNMMFQKDTFAYAEDEKAQYLDMPYGNKAFSMTVILPKNGNTT
                     ADLLEYLTVDQWNSILQNLSMREVEVYFPRFSSKNKFLLNDPLIDMGMKLAFTDNADF
                     TNIANENLFISRILHNTYIEVTEEGTEAAAVTVVEVGYTSVPIIPEFRVNKPFLFVIR
                     ENSTGVILFMGKMGNMELF"
BASE COUNT         5741 a         3198 c         3815 g         5583 t
ORIGIN      
        1 cagaaatgtt gatcctctgg agcttttttc ttgtattaaa gtattatctg tgtgtttttt
       61 tctgaaatga atatgatttt atggctacac aatttatgtt gaatggtgaa aaattaaaaa
      121 ttttatgtaa gtttgtatgt tgtaaaataa tagaatttca atttttttgt ttttgtttta
      181 gtggagaata ttttttatta aacaatctct tatattattg attaatttga ttcacgataa
      241 ttgttgaaaa ggaaaaattt aaataaaata ctgtttctgc tgatcataat ttttgtgagc
      301 agttgttcaa tgcagaaaaa cacatggttt tctcgtaatt atcatgcgat gactgcttat
      361 tttaatatct atttcaatgg gctggaaagt tacaacgagg gattgaaaaa cattttggtg
      421 gctcaccagg acgattattc cactattctt ccgatgtttc ccattagtaa acattccaat
      481 gcttcggctg ctacggcaaa tatggatcgt gccatagaaa aatcgagaaa agccataaag
      541 cttcactcca tcaagcaaaa acccgagaag aattacaata aatggaattc tgccgaatac
      601 caggcttttt ataatcagga agaattcaat cctgttttaa aagatgcatg gatgcttatt
      661 gggaaagccg agtttcataa aggcgatttt ttgggttcgg ccggaacatt ttcctatatc
      721 gcccgccatt ttgcttctga tcctgatttg gttgcacaag ccaaaatttg gttggctcgt
      781 gcttatggtg aaatgggttg gctttacgaa gctcagcaag ttttgggaga tgttaaccaa
      841 gaccatttaa aacgccagca tatcggtctt tttgcttctg ttaatgcaga tattctgttg
      901 aaaaaccatc agtacaaaga agcgattcct tttttgaggg ttgcaattga caaggaaacc
      961 gacaagaaat tgaaacaacg gttccttttc attttggctc agctttatca gaaaacgggc
     1021 gacgaaaaag cagcctacga aacctatacc aaagtgcttc aatccaatcc gcctttcgaa
     1081 atggattttt acgcacgcat tcagcgctcg gaactgaaag cagccaacca tgcagaagtg
     1141 cagaaagatt tgaacaaaat gcttaaaagt tccaaatata aagattatca ggatcagatt
     1201 tattatgttc tcggcaaaaa tttcttgcat cggggcgaca cggctcaggc cataaatgct
     1261 ttccgttcct ctattgaaaa aagcacccga aatggttatg ataaagccaa ttctctgatt
     1321 acgctcggag atttgtttta tcatcagaag aattacaagg aagcatatcc ctgctatgat
     1381 gctgcttcca aaattattgc tgaagaacac cccgactacc ggcgtatttc aaaattagcc
     1441 gacattttga gtgaatttgt aacgcaatac gatatagtaa tgttgcagga cagtttgcag
     1501 cgtttggctg ccatgcccga aaaacagcgt atggaagtga ttcaaaaaat catcagtcag
     1561 gttgagaagg aagaaaaggc ggctgctaaa aaagctgaag aagagttgct gggattgaac
     1621 tatcccaata atgacgatga agaaaccctc atcccaattg gtgctcaaat gggtaattcc
     1681 aacgactggt atttttataa tcaaaatttg atgaaagttg ggaaaagtga gtttcagaaa
     1741 aaatggggca accgaaaatt ggaagacaat tggcgtcgtg ccaataaatc ggcttcgctt
     1801 tttgcagagg aacctaacgc cgaaaacgtc agcagtgaaa tgatgatgga cagcacgtca
     1861 tcaacattgg ccatcaccga caataaaaat ccggcatttt atctgaaaca aataccgctg
     1921 acgccgcttc aattgaaaat ttcaaatgag cagatggcgc aggcaatgtt taatgtggga
     1981 aaaatttatt atgaaaagat tcaggatatt ccttctgcaa ttcggacttt tgaggaattt
     2041 gtcggaagat ttcctgatca caagctcgtt ccagatgctt attttcagtt gtatttaatg
     2101 gaatctaaac tcggcaatct ggctaatgca gaaaaatggc gcaacagact tgtgggtgaa
     2161 tttctacaaa ccgactattc aaaaatgctt tcaaaaccgg attatattca acgtttgcaa
     2221 cgaatgtatc aagagcagga ttcgatttac aatgctacat atcgggctta ttcgagtaat
     2281 gatttcgcta ctgttttccg aaatttccaa tatatgcaag aaaattatcc cttgtcggaa
     2341 ttgatgccta aatttcagtt tctttatgcg ttgagtgtgg gcaaaacaca agaatcgcaa
     2401 aactttgaaa aagaactgga tctgttggtt tcaaaatatc ctgaaagtga tgtcagtaca
     2461 atggcgaaag atattttggc gctgatgcgt caggggatgg tagcaaaatc cggtactaca
     2521 cacgggtcat tgcttgccaa aagggaagag gcggagaaat tggaaatttc gccatctgcg
     2581 cagcagcaat tttctacaga taccaaaacc aaacataggc ttttgttgat tacggatgct
     2641 cctcgagaag aactgaacaa gttaatgtat cagattgctt ccttcaactt ttcccgtttt
     2701 ctcatcaagg attttgattt gggaattcaa ccgttggaca gtgtcagaaa tatattatat
     2761 gttgacaact tcgattctta cgacgaagtg gtttggtatg aaaatacaat ggcttccgat
     2821 gcttatttga ctgagttgct ttcacgattg aaagtgcagg aattgataat ttccgatgaa
     2881 aattttgctt tgttgaaaaa tggattatcg ttagcggatt atatggcttt tcatgataaa
     2941 tttttgggca aaaatgccgg ctctgcttcc aaacaaatat ccgcgttacc tgaaaaagaa
     3001 aagaaatctg aatctcccaa aactattgcg gcgacggcaa acaaacccaa ttcggtaacc
     3061 gaaacaaaaa aaacagtttc gacttcgacc gaaaaaactc ctttggttgc accaactcaa
     3121 ccggcagaac aaacgccaaa cgttgcttct gttgccgaac aaccggtgaa aaacgagcct
     3181 gttgaaaaag ttgaagtcaa gaaggaagaa gaagttccgc tgttcaaaaa tctgtttgcc
     3241 tataaaccga acgatccaca tttcattgcc gtttatgttg tatcgggaac ggtagatgtg
     3301 gcaaaaacca aagcagcttt tgacgagtac aaccgacaaa attacgatgt gctgcaactg
     3361 aatgtgcgat ttgaaaacgt gggcaagcag caatttttca ttataggcag cttcaccgat
     3421 gccaatactg ccaaatcgta tttgttgcgt atggtgaagg aaagcaaact gtttgaagga
     3481 ttaaaaggtg caagttacag aaatctggtc ggaacgcagc agaacctgaa tgttatggta
     3541 cagcaaaatg cgctgaacgt atatttcgag tttatgaaac aatattatct gaactgaaaa
     3601 gttttttgag aaaaggaaat tgaaggaaat gagaaaagag agaatacttt ggatagatga
     3661 cgaaatcgag atgctgcgac cttatctcct ttttttggat gaaaagggat atgaagttgt
     3721 tacggctacc aatgggaatg atgccgtaga aatctgtcag gaggaaaatt tcgatattat
     3781 ttttctggat gaaaacatgc cgggattgag tgggctggaa acgctggcac tgattaagga
     3841 aatcgacccg gatgttccaa tggtgatgat tacaaaaaat gaagaagaaa acatcatgaa
     3901 tatggctatt ggcaataaaa ttgccgatta tttgacaaaa cctgtcaatc caagtcagat
     3961 tctgatgacg ctcaagaaaa atattcatcg aaaggaaatc gtaaacgaac atgcaacagc
     4021 tgcctatcgt tccgaatttg gacaaatagg catgaaaatc aacgattcat ttacatacga
     4081 cgattgggta gaagtttaca aaaaacttgt tttctgggaa ttggagcttt ccgaaaccga
     4141 aagtgggatg gatgaattgc ttcgaatgca gaaaatggac gccaatacaa ctttttcaaa
     4201 attcataaaa aacaactacg aacgatggtt cgagcgcccc gaagaacgtc ctttgatgag
     4261 ttttgaaata tttaagaaca aagtttttcc cttgcttgat aatggcgaaa aggttttttt
     4321 tgtggtggta gataatttcc gcctcgatca gtggaaaatg atacgtgaat tgttgagcga
     4381 ttattatcag tttgacgaag aagatcttta ttgcagtatt cttcctaccg ctacacaata
     4441 tgcgcgaaat gccatctttt ccggtctgat gcctctgcaa attcaacaga tgtttcctca
     4501 attttgggtg gaagaagaag aggaagaagg taaaaaccta atggagagtg agttgcttca
     4561 gacgcaaatt caacgctatc ggaaaaaata tactttttcg taccataaaa ttaacgattc
     4621 cgtttcgtgt cagcatctga tcgaccagtt gccacatttg gaaaacaata atttgaacgt
     4681 ttgtgtactg aattttatcg acatgctttc gcatgcacgt acggaatcaa aaatggttcg
     4741 cgagttggcc aacgacgaaa aagcttatcg ctcactcacc ctttcatggt tccgtcattc
     4801 tgctactttc gatttgttca gggctattgc cgaacgcggt tacaaagtgg tttttacaac
     4861 cgatcacggc accattcgag taaataatcc cataaaagtc attggagata aaaatacaaa
     4921 tgtcaatctg cgctataagg tagggaaaaa tttgagctac aacaaaaagg aagttttcga
     4981 aattacccaa cctgcaaaaa ttggcttacc aagccccaat gtcagttcgg cttacatatt
     5041 tgcctgtaag gatgattttt ttgcttatcc gaataattac aattattacg tgagttatta
     5101 taaaaatacc tttcaacatg gcggcgtttc tttggaagaa atgttggtgc ctctaattgt
     5161 gatgaatccc aaataagtgg atgaagttaa atctttttcg acagcggttg aaaaagagta
     5221 atttgcagta atatttttat ttataaacga catcaggtga agactctgat gaaaaaacga
     5281 aatactatga gaataggatt gataggattt ggaaaaacgg gaaaagccgt tgcttcagtt
     5341 attttacaaa gcgagagttt tgaactgaaa tggattctca ggcgtaaaaa actactcgat
     5401 caccgttctg ctgctgaagt cttgggtata gaaaccagca gtacggaagg acttatttat
     5461 tcgacttcaa atacgaatct cgaaaattta atggatactt tacccgtaga tgccatcatt
     5521 gatttttctt caggtcaagg tatttatact tatggagagc tggcggcaaa gagaaaaatt
     5581 aaaattatct cggctatttc tcattacaaa ccgaaagaag ttgctttttt gcataagttg
     5641 gctcaacaga ctaccgtttt ctggtcgcca aatattacgc ttggagtaaa ctatttaatg
     5701 atttctgcca aatttttgaa aaaaattgcc ccttgggttg atattgaaat agtcgaagag
     5761 cattttaagg ggaaagatgg aatttcaggt actgccttga aaattgctga agtgctggat
     5821 atggataaag caaaaatcaa ttccattcgt gcagggggaa ttgttggccg acacgaaatc
     5881 atttttggat ttccttatca gaccgtcaga ctgattcatg agtctatttc gcgggaagtt
     5941 ttggtaatgg agttttgttt gttgccgaaa atttaaagga caaggaagat ggtttttata
     6001 attttcaaga tttattgctt ccttattttt atgattaatc aaaaagttgc attttcaact
     6061 tgaatgttgc aatagaaaaa atcaaatttt ttctattaac ttgaaatacg aagaaatttt
     6121 gaaaagtatg aaaaaaattt tatttgtgct tatgacagta gcttttttag gatgtcgttc
     6181 cgaaaaaccg gcaacgccgg cgatggaaac atttccttac acggttgacc gatttgccga
     6241 tattgaaatt ctgcgttatc gggttcccga tttcgaaaca ctaactttgc aacagaaaga
     6301 actcatttat tatctttcag aagctgcttt atcagggcgc gacattttgt acgaccagaa
     6361 caacaagtac aatttatgtg ttcgtcgtac attggaagct gtttacgaaa attatcgcgg
     6421 agatcgccag tctgaagaat tcagaaattt tattacttat ctgaagcagg tttggatggg
     6481 caacggtatt catcatcact attcgcagga taaatttgtt ccaaagtttt ctgcggcttt
     6541 ttttgctcag gcagtgaaaa gtattgatgc tgaagttttg ccgctcgaaa agggcgaaac
     6601 tcccgatgat ttgattcgta aactgactcc tgtgattttc gatccgacag tttttcctaa
     6661 acgtaccaat caggctgacg gagtggattt attgcttact tcggcagtta atttttatga
     6721 aggcgttaca caagctgaag ctgaagaatt ttacgaccga atgagaaatc cgaatgattc
     6781 aactcctatc tcttacggac taaacagtaa actgatgaaa gaaaacggtg ttttggtaga
     6841 aaaaacatat aagttgggag gaatgtatgg taaagccatt gagcaaattg tttactggct
     6901 caataaagct ctttctgtgg ccgaaaatga taaacaaaag gacgtcattc attcattgat
     6961 aaaattttat gaaaccggag atttgaaaac ctatgacgaa tattgtataa aatgggtgca
     7021 ggatactgcg tcattggtag attttgtaaa cggtttcacc gaaacctatg ccgatccgct
     7081 tggattgaaa ggcagttggg aatctattgt gaattttaaa aatctggctt ccaccaaaag
     7141 aactgaaacc atcagcgaaa atgcgcagtg gtttgaaaat aattcgccca tcgataatcg
     7201 ttttaaaaag gaaaatgtga agggaatcac agcaaaagta gttactgcag ccattttggc
     7261 aggcgactgc tatccgtcca ctccgattgg cattaatctt cccaattccg attggatcag
     7321 gcacgactat ggttcaaaat cggttactat tgaaaacatt acggaagctt acgaccgggc
     7381 ttctttggga aacggattca acgaagagtt tatgtggagc gacgtggaac gcgaaagagc
     7441 taaaaagtac ggttcgttga ccaataattt gcacactgat ttgcatgaat gtctggggca
     7501 tggttccggg aaattgcttc cgggcgtcga ccccaatggg ttgaaagctt atgcatcgac
     7561 cattgaagag gcacgtgccg atttattcgg attgtattat gtggctgatt caaaaatgct
     7621 cgaactcgga ctgttgcccg accatgaagc ctataaagcc gagtattata agtatatcat
     7681 gaatggcctt ctcactcagc tgacgcgcgt tcagacaggg aaaaatattg aagaggctca
     7741 catgcgcaat cgtcaactta ttgctcgctg ggtgtacgaa aaaggcttgc ccgaaaatgt
     7801 gattgaactt aaaaaaagag atggaaaaac ttacgttgtg gtcaatgatt atgaaaaact
     7861 gagaattctc tttggaaaac ttctggccga aattcagcgc attaagtcca ccggcgatta
     7921 caatggtgca aagactttgg tagaaaatta cggagtaaaa gttgatcctg ttattcacaa
     7981 ggaaattttg gaacgctacg aaaaattaaa tcttgcacct tatcgtggtt ttgtcaatcc
     8041 tgtttataag gctataaaaa atgccgatgg gaaaatcagg gatgtgaaaa tttcttatga
     8101 cgaaaattat gtggagcaac aattgaggta ttcaagacaa tattccaatt taccgacatt
     8161 caattgaaaa gcattttttt gtaattgttt gttgaataat agtttaataa aatttcaaag
     8221 gaattttttt cgtaaaggaa cttactttgt tggttattgt cgccatgaca ggaagtaagt
     8281 gtaacaattg ttgtggataa tgcctaaatt tggctaaaaa actgttgata tttaaggaat
     8341 ttttccgaac tttgcagcct aaaaatgaat aacattgtga aatgaatacg aacaacgaaa
     8401 aatggcatgg gagtccaaaa aaagggggga actcttatga cagccgtgag ggcagacatt
     8461 ttgaaaaagg cggcaataat tcgcctttca gtaaaccggg aaggaaccaa aaggtttatt
     8521 cttctcgaaa tacctccaat tccaattatg acaatcgttc taccggaaag gaaccctatt
     8581 ataagaacga aaacaaaaaa ccatacaggc gaccttttac tacggatgaa aattcggaag
     8641 gtgaaaatcg caaattgaag ctggtaaaac ccggtaagtt tgttgaaaca cgttttgatg
     8701 accgacaacc caatgtaact tttcgtaaac gaaaaaacaa aaccgaaggc gagtttaatc
     8761 ccaatgataa atacagtctt aaaaaacaac tcgagtatca aaaaaataat gtcgattatt
     8821 caaaacctat tcggttgaac aaatttttgg ccaatgccgg tatttgttct cgccgcgaag
     8881 ccgatgaatt tattcagtcg ggtgtcattt ctgttaatgg tgaagtggtt actcagctcg
     8941 gtgcaaaaat tatgatgacc gacacagtga tgtttaaaaa ccagcccgtg cagtcggaaa
     9001 gaaaagtgta cctattgctc aataagccga aagattgcct gacaacttct gatgatccca
     9061 ataaccgcaa aacagtgctc gatttggtaa aaaatgcttg cagtgaaagg atttatcccg
     9121 tcggaaggct cgacagaaat accactggtg ttttactgct cactaacgat ggtgatttgg
     9181 cagccaaatt gacccacccg aaatacaaca aaaagaaaat ttaccatgcc gttctcgaca
     9241 aaaatcttga gacagtcgat tacgaatcta ttatacaagg tgtagatttg ggagatgaac
     9301 atgtgtcggt cgatgcactc gaatttgtaa aagccggcaa tcgaagagag ataggaatag
     9361 aaattcattc aggacaaaac agggtagtaa ggcgaatttt tgaaaaattt ggttataaag
     9421 tcgtatatct tgaccgtgtt tatttcgccg ggttgaccaa aaagaatgta cccagaggaa
     9481 aatatcgctt tctgactcag cgcgaaatca atatgctgaa aatgggagct tacgaataaa
     9541 aaagttctta tgggtttttc ttatgaattt ttttaaaaaa ttttatacta caatcattta
     9601 aaaccaaatg tgatatgagt gaagaaaatt tgaacaatac tcgttctgac gagcaaagtc
     9661 ccgtttcgtc gcagcctaca aataaaaaag gttttttccg aacatttatt tcacgtcata
     9721 cgggagtttt cgtattgttg tgtttgctga tagctgtagt tattgctgcc tttattcgta
     9781 ttaaattgtt ggaacgcagt ttcaacaaac aacaggaggc tttgcgggtt cgatacgaaa
     9841 ataaagtaga cagccttctt caagcggaca ggctgacaac ggccaaagtt ttttcgtggg
     9901 cagtgagggg cgagctgata cgtgataacc gagatcagat aaatcagtat tttatgaatt
     9961 ttgttaagga atccggcgta aagaaaatta aactcattga ccctgtttct gcaaaagcaa
    10021 ttatctctac cgataaaaaa gatgaaggtt tggtttttgc cgatcccact ttcttcactg
    10081 aagaaattgc agttcgccat cttgattccg taactcaaat tatttctccg gttatgggac
    10141 tgaataaaaa aattggcatt ttggtaatag aatacgaata aatttctttt ttttttgaca
    10201 tgtacaaaag actgtttaaa attttttttg atcagccttt ttatttttgt tctgaatttt
    10261 ttttgtttca attttaaaaa gaagcgggga aatcatttcg tctttttaaa ataaattttc
    10321 tttgtacctt tggattttct taagtatttt cagcaaaaaa gctaaaaaat ttttccatga
    10381 atgatttaat gaaacgattc aaatctctca acgttatttt tggctgggta gcatttgcta
    10441 ttgctactgt aacctatttg ctaactttgg agccaaccgc cagtttttgg gattgcggcg
    10501 aatttatatc cacagcttac aagctcgatg tgggtcatcc tcccggagca ccgtttttta
    10561 tgctcacggc aagatttttt acgctattta cttccgatcc cacacgcgtt gcagttatgg
    10621 taaatacttt gtcggctttg tgcagtggct tgactatttt attccttttc tggaccatta
    10681 ctcatctggc tcgcaaggtt gtgatcaagt cggacgaaga ttatactacc ggcaacatta
    10741 tcatcatctt aggtgccgga ctggtgggtg ctttggctta tgctttttcc gatacattct
    10801 ggttttcggc tgtggaggca gaagtttatg cttattcttc actgtttact gcagtggttt
    10861 tttggctgat attgaagtgg gaagaagttg cagatgagcc gggttcggat cgatggttgt
    10921 tgctgattgc ttatttaatg ggtttgtcta ttggtgttca cctattaaat ttgttggcaa
    10981 ttcctgccat tgtgctggtt tattatttta aaaaattcac accatctctt aagggtgttt
    11041 tgtttgcttt gcttggttct tttctgattt tgggagtggt tttgtatggg attattccag
    11101 gatttgttca ggtggccggt tggtttgaac ttttgtttgt caataaattg ggaatggctt
    11161 tcaattcagg gctttatgtt tatattgttc tttcaattgc ggcacttact tgggctattt
    11221 acgaatctta cgtcaacaaa aactatttaa ggattgtatt gtctttcctc gtggccatta
    11281 ctttggtagg cattccgttt tttggacagc atattgtgct gggcattatt cttgttgccg
    11341 gcttaagctt ttatttttat aaatggaaaa ataaaatcaa tgttcattgg attcatactt
    11401 ccattttgat gataacttta atgttgattg gctattcgac ttatagcgtc attgttattc
    11461 gttctgccgc caatccacct atggatcaaa attcacccga caacgttttt tcgttgcgtt
    11521 attatttgaa tcgtgagcag tatggcgatc ggccactgat ttatggacct gtttacaatg
    11581 caccggtgaa gttgcgcatt gatggtaata tgtgtattcc tgaacaagag ttgggagaag
    11641 caatgtatgc accaaaacca aaaaaatcgc ccaacgaaaa agatcagtat atgcttacag
    11701 ggtataaaat ggactataag atggacgaaa ggttcaatat gttattccca cgtatgtaca
    11761 gcaccaacag ttcgcatgtt caggcataca aaatatgggg caatgtaaag ggtaaaccca
    11821 taactttcga ttattgtgga cagcagcgca cagaaatgaa acctactttt gccgaaaatc
    11881 tgcgtttctt tttcgattat caattaaatt tcatgtattg gagatatttt ctttggaatt
    11941 tttccggccg gcagaacgac ttgcagggaa atggagaagt tgataaggga aactggatta
    12001 ccggtatcaa ttttattgac aagtatctgg ttggagatca aacgaatctt cctacttttc
    12061 ttgcagaaaa taaaggaaga aatgcttatt tcatgcttcc cctcattttg ggtattcttg
    12121 gaattttatg gcagctttac ggtggaaaaa atggtaagca aactttttgg gttacctttc
    12181 tcttattctt tatgaccggg attgccattg tggtatattt gaatcaaact ccttatcagc
    12241 ccagagaaag agattatgct tatgcaggtt cattttatgc ttttagtatc tggattgggt
    12301 tgggagtttt agaaattttc aaattcataa ataaatacat accgaagacg ccaagtgcta
    12361 ttcttgcttc tttactttgt ttgggagttc ctgtactaat ggcttccgaa aactgggatg
    12421 accatgaccg gagcggacgt ttcacagctc gcgattttgg gcaaaattat cttgaatcgt
    12481 gccgaccgaa tgccatcatc ttcaccaacg gcgataatga tactttccct ctgtggtaca
    12541 atcaggaagt ggaagggaaa agaaccgaca ttagagtttg caatttaagt tatttgcaaa
    12601 ccgactggta catagatcag atgaaacgtg aagcttacga atcagctcct ctacccattt
    12661 cttgggaacc tgaagattat atttcgggta aaaatgaagt gatttcggtg gaagatttta
    12721 ttaagcagcc cattgatgtt aaaaccatca tggattttat tcggagttcc gaccctcaaa
    12781 ccaaaataaa tggtgaagcg gttattccga ccaagcaaat ttacattccc gttgatgcac
    12841 agcaggtagt aaaaaccgga accgtcaaac ctgaaagagc agccgaaatt gttccaaaaa
    12901 ttgatttcaa cctgaaaaac aggctgacaa aaagcgaatt gatggtgatt gaaatgttga
    12961 aggaaaataa ttgggaacgt cccatgtatt ttgccgtaac agtaggagat gattattatt
    13021 taggattaaa agatcacttt gaactcacag gtatggctta tcaaattgtg cccgtaggtg
    13081 cgaaggatgc aggtcctggt gttaatacgg atgaaatgta cgaaaatgtg atgcataaat
    13141 tccggtttgg aggaataaat aatcccaaag tgtatataga tgaaaacatt ggcagaatgt
    13201 gtcgcacaca ccgcatgttt atgggacagc tggctggcgc tttacttgct gaaggcgaca
    13261 ccgtaaaagc taaagcagtg cttgattatt gtttggagat gattcctgga aactctgtgc
    13321 gtcatgatta tacttccgtg caaatagcaa attattatta tcgcctggaa gaatttgaaa
    13381 aaggtgatgc catcatggat agcgtggcta aagattgtgt agaatatata aattggggtt
    13441 ttagtttaag tccttcaaaa caaaacagca taacgggaga cttgggaaat aacatggcag
    13501 tgctaagtca gatactgcaa atttgcgatg agctaaaacg gaaaaatatt cttgataagt
    13561 atcttcctgt ttacatgcaa tattcgcggt attttcaaat gtgaatgagc tgtcaatttc
    13621 ctgctttttt gcgtccgctg ataggacgaa attatttgtg gagaaaaaat tcttcggaaa
    13681 aagttgtcta tctgactttt gatgatggac cggtgcctga ggttacgcct caggtacttg
    13741 gtgtacttga tgattttgac attaaagcta cttttttttg tgtaggcgac aatgtgaaaa
    13801 aatatcccga actttaccgg gaaattcttt tgaaggggca cagcgttggg aatcacactt
    13861 ttcatcattt gaacgggttt aaagttgggc aagagcaata tctgaaagat gtggaacagg
    13921 cttcgcagtt gattgacagc cggttgtttc gtccccctta cggaaaactc actcgcaaag
    13981 agaagaaaat tttggaacaa aaatttcaaa ttgtcttgtg ggatgtgatt tcgtatgact
    14041 atgaccaaaa tctgtcgtct tcacaagttt tgaagaatgt gaaacgctat acaagaagtg
    14101 gttcggtgat tttgtttcac gattccgtga aggcaaaaaa caatatgctg acggttcttc
    14161 cacaagccat aagttttctg ttggcaaaag gttatcgttt cgaaaaactc tgatgtattt
    14221 tttgtttttc tcaataattt attgattcaa aagtctgtat ttcttaactg aaaatcgctt
    14281 ccatgaaaga atctattttg caatatgtat ggcagcataa gcttttttta caaagtaatc
    14341 tgcaaactgc tgatggagaa aaagtggaaa tcattgatgt gggcaaaagc aatacggatg
    14401 cggggcccga tttttttaat tctaaaataa aaatcgacgg aaccctttgg gtgggtaatg
    14461 tggaaattca cctccgttct tcggaatgga taaaacacgg acaccagaaa gataaagctt
    14521 atgataccgt aattttgcat gtggtggcag aaatggatgg agaagtcttc agaacggacg
    14581 gaacaaaaat tcctcagctg gttttaccat tttcaaaaga tattgaagcg aattacgatt
    14641 cgttgttgaa caataataaa tggatccctt gcgcagataa aattgagaaa atttccgaaa
    14701 tttttattca aagttggaaa aatgctttgc tggtggagcg attggaacaa aaaatgacag
    14761 agatagaaaa tttgttgaca caaaatcatt atcattggga agaagctttt tacattataa
    14821 tagcgcgaaa ttttggtttt ggcaccaatg gtcaggcttt cgaactgttg gcaaaatctg
    14881 ttccgctgaa tgtgttggcc aagcataagg ataatctgtt gcaattggaa gccattttgt
    14941 tcggtcaggc aggtttgctt cctgttgata catcggatga tgaatacgct atgcaactga
    15001 taaaggaata cgcttttttg agaaacaaat tttcgttaca gcccatcgac tgcagccaat
    15061 ggaaattatt cagactcagg ccttataatt ttcctcatgt tagaattgca caatttgctt
    15121 ccctgattca tcagtcatca aagcttttct caaaaattat cgaaactccc aatttggaat
    15181 tgatgaaaga cctgttttcc tgcaaacctt ccaattattg gcaaacgcat tatctgtttg
    15241 gctctgcaag tccaaaaagc accaaacagc tcggattgta ttctatctac agcattgtaa
    15301 tcaatacaat tgttcccttt ttgttttgtt atgcttcaaa aaagaacaac gaagatttaa
    15361 aagataaatc tttgcatttg ctggaagcca ttccttcaga aaaaaatgcc attgtcaaag
    15421 gttggagaaa tattggaatt gcttccgaat cagcatttga ttctcaggct cttatacaac
    15481 tgaaaaagca atattgcgat acgaagaaat gtttgttttg ccgaattggt cacaaagtat
    15541 taacgttgca aacaacctga cttgaaacaa caatgagatt aaattttttt aagacgatcg
    15601 ctttttcagg aataatttta tcggttgttg gctgtgccaa caaagcacaa ggcccaaccg
    15661 gcggcccaaa agactccatt ccaccaagtg ttgttcgctc tgtcccttcc aacggtgcgt
    15721 taaattttaa gaaaaaacaa attctgatcg attttgatga aaatgtcagt atagaaaaag
    15781 cggccgaaaa tgtactgatt tctcctcctc aggtgaaacc gcccgaagtt gttgccatcg
    15841 gaaagagagt tacggtcaat ttgcaggacg atttaaaaga cagtacaacc tacactatta
    15901 atttcgggaa tgccatagta gatttgaacg aaaaaaatcc attaaaaaat ttttctttct
    15961 cgttttctac cggaaatcag atcgatacct tgcaagttaa gggaactgtt atcaatgctg
    16021 aagatttgaa tccggtttcg ggagttattg ttggtgttta taatgattta tccgatacgg
    16081 tttttacaca aaaacctttt ttgagaattg ggaaaacgga tgaaatcggg aagttttcag
    16141 tagaaaattt gaaaaaggat actttcaaaa tatatgcttt gggcgacgcc aacagagatt
    16201 atttttatca gccgggtgaa gggcttgctt ttaccgattc gctgattgtt cctggatttt
    16261 tcagagaaga agtgatggat acgatttgga aagattctgt tcaaatcgat accattcgct
    16321 ccattgtacg aacacatttt ttccctgata acatcctgct gaaatatttt aaggaaaaca
    16381 aaaaaagaca atatttcatt aaagctgaac gaactttacc ttatgctttt tctttgttct
    16441 tcagtgcacc catgaccgaa ttgccaacct tgaaaccaat tgattttgaa tgggatggca
    16501 aatatattct tcagaaaaac aatacgatgg acagtctgac ttactggatg accgattcgt
    16561 tggtttggaa gaaagacacg ttgcagtttg aaatgagtta tcagaaaacc gattcgcttt
    16621 ttcgacttgt acctgcaacg gatacattga aaattttcat gcgaactgct cgctctactg
    16681 tgagaaatac caaagacaaa aaagaacctg tttcacgggt aaaaatggag caaaattacc
    16741 agtttacaac caatattgcc ggttcttttg atatttatca gcctgttgtt tttcgtttca
    16801 gtacgcctct ggcttatttt gattcatcca aaatttcact tgctcaaaga gtagatacga
    16861 ttttaaaacc tttgcctgta aactggcgac aggtagattc taccaaaatg gtttatgctg
    16921 tcgattacaa atgggaaccg gagaaaaatt atgagttgac agtcgattcg gctgctttta
    16981 ccagcatata tcagcaaacc agtaagaaat attccggaaa atttaaaatt cgttctttgg
    17041 atgaatacag cacggttatt atggttttgg aaaattttgt tcccagcgcc gtgatccaaa
    17101 ttttggatgc caaagatgcg gtgattgctt caaagcaagc acaggaaaaa ggaaccgttt
    17161 ttcagtatct gaaacccggc gattattatg ctcgactgtt tttggatgag aatggaaacg
    17221 gcaaatggga taccggagac tttgctgctc acaggcaacc ggaagcggtt ttctattatc
    17281 cgaaaaaaat gactctaatg cccaattggg aatttgaaga aacatgggat tatcttaaag
    17341 tgcctctgct tgaacagaag cccaaggaaa ttcgcaaaga tgccggaaag aaaacatcgg
    17401 ggaattgagt tgaggaaaag ttaccgaatc attttcagaa catcactcat caaaacaatt
    17461 ccatattccc cattttgccc ataaagagaa tgacacccgt actgttttcg cgaatcacaa
    17521 ataaaaatgg tttgtttact cgaaattcgg gaatgatggg aacggaagta tatccaactt
    17581 ctactaccgt tacagcagcg gcttcagttc cttcttccgt aacttcgata taagtattgt
    17641 ggagaattct cgaaataaaa agattttcgt tggcaatgtt tgtaaaatcg gcattatcgg
    17701 taaatgccaa tttcatgccc atgtcaatca gcgggtcgtt taaaaggaat ttatttttac
    17761 tgctgaaacg tggaaaataa acttctactt ctctcataga taaattttga agaatgctgt
    17821 tccactgatc tactgtaaga tattccaaca aatctgccgt tgtatttcca tttttcggta
    17881 aaattaccgt catgctgaaa gctttgtttc cgtaaggcat atcgaggtat tgagcctttt
    17941 catcttctgc ataggcaaaa gtatctttct gaaacatcat atttactttc actttttcgc
    18001 ctgcttcgtt ggtaaaatca gtttgtttag tgtttttctt ctcgaattga tgacgccaga
    18061 ttcccttgaa ataaatggca ttgatcagat acatcactgc ttcatcggga attgcatcca
    18121 gtaccgaagg aatcaaatta ttggtttttt tagcacacca ttgattaatg gtatccaccg
    18181 cccaagtttt tgaaaaatcg agttctcgta catacgcatt aaaataatcg atattaactt
    18241 tcaaaaaatc cggttttacc tgaaaaccgc tgcgatacca caaagaattg gcaagagtga
    18301 gtttggtggt cgggtcaatg gttggaagcg tggtttg
//
LOCUS       DAOTAX010000043        17357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2723_length_17357_cov_31.844700, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000043 DAOTAX010000000
VERSION     DAOTAX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17357
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_17357_cov_31.844700"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1875)
                     /gene="hcp"
                     /locus_tag="PLM04_05730"
                     /gene_synonym="priS"
     CDS             complement(220..1875)
                     /gene="hcp"
                     /locus_tag="PLM04_05730"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HON02609.1"
                     /translation="MFCYQCQETSKGTGCELKGVCGKTSDVAALQDLLIYQLKGLSNY
                     TTALRKVGVESPLVSRFIIDGLFMTVTNVNFDKQRFVEQIREGFDLRSQLKQDLSIHG
                     ITLPASELTEWFADTEADMENMASKVGVLSTENEDVRSLRELIIYGLKGMAAYAKHAM
                     NLRFTDGEINAFVEETLVKTTDDTLSMEELINLVLETGRFGVKVMELLDKANTTAYGH
                     PQVTKVSLQTREKPGILISGHDLKDMEELLKQTEGTGVDVYTHGEMLPAHYYPAFKKY
                     DHFVGNYGGAWWNQTQDFETFNGVILFTTNCIVPPRSNSTYADKVYTTGAAGFPGFKH
                     IAEAPEGKAKDFSSLIEHAKQCAAPIQIEKGEIVGGFAHEQFFALSDQIIEAVKSGQI
                     RKFFVMAGCDGRLKSRNYYTEFAEKLPKDTVILTAGCAKYRYNKLALGEINGIPRVLD
                     AGQCNDSYSLALIALKLKEIFNLNSINELPIVYNIAWYEQKAVIVLLALLYLGVKNIH
                     LGPTLPAFLSPNVAKVLVDNFGIAPIGSVEDDLNMFLNLENFN"
     gene            complement(1935..2291)
                     /locus_tag="PLM04_05735"
     CDS             complement(1935..2291)
                     /locus_tag="PLM04_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HON02610.1"
                     /translation="MENSEANNRQESKTIPAGEKFVLANQVNYADGSVVSKIIVRNRQ
                     GNITLFAFDKGEFLSEHAAPFNALVVALDGKGTITISGQPFELISGESIILPAGVPHA
                     VNATERFKMMLVMIKD"
     gene            2462..3124
                     /locus_tag="PLM04_05740"
     CDS             2462..3124
                     /locus_tag="PLM04_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HON02611.1"
                     /translation="MKSIDPEWKSYGSIDDGESGFPADLKYSIKPYRKNEIMFRQGSI
                     CDALYILTSGSVKTEMITETGNIMGIELIKAPRPLAPAFLFSDNNRFPVDVTALEDCE
                     ILRIPKEEVMRLMAIQPDFMQQFLKHNANRAEFLANRLQLLSIKTIKGRIAHFLLEQM
                     AEYGKTFTINRNRTELAEFFGVARPSLARSLSEMVEDNIIKINKKQFTVIDERKLKDL
                     LL"
     gene            complement(3159..6005)
                     /gene="gcvP"
                     /locus_tag="PLM04_05745"
     CDS             complement(3159..6005)
                     /gene="gcvP"
                     /locus_tag="PLM04_05745"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HON02612.1"
                     /translation="MKDFLSFRHIGVSSDEIQEMLEVVGAESLDELITQTIPLSIRLN
                     EKLNLPSALTENQYAQHISEIAEKNKIFTSYIGRGWYDTCTPAVILRNVFENPSWYTS
                     YTPYQAEISQGRLEALLNFQTAVCEFTGLPLANASLLDEATAAAEAATMMYNLRSREQ
                     MKNGANLLFVDENIFTSTLAVLQTRTQAQGIQLEIGDYKSVQFSEKHFGAILQYPNAD
                     GNVEHYFSFVSQAHTVGCKVAVAADILSLALLVPPGEWGADIVFGSTQRWGIPMGFGG
                     PSAAYFATRDEFKRDMPGRIIGISKDKNGKPALRMALQTREQHIKREKATSNICTAQA
                     LLASMAGFYTVFHGPEGIRNIAKNIHAIAAYINAMLPTYGYRQLNKLFFDTLKIVLPE
                     NVSQEEFRNIALSHKVNFYYFETGEVGLSINETTEAPDIQRLINIFSEAVHKKPIVFN
                     PTDLSEFEIPDEKIRRKSAYLTQEIFHRYHTEMEMMRYIKKLERKDISLTHSMISLGS
                     CTMKLTAASEMLPLSKAEFLKIHPLAPSDQVKGYIELIGELEKYLCVITGFDACSLQP
                     NSGAAGEFAGLMTIKAFYRSRGETERNVVLIPASAHGTNPASAHQAGLEIVVVDCDEN
                     GNIDLNDWKVKAETYRTRLLGCMVTYPSTHGIFEAHIGEMCQIIHANGGQVYMDGANM
                     NAQVGLTHPAKIGADICHLNLHKTFAIPHGGGGPGVGPVCAAAHLAPFLPGKTSGQNT
                     VSAAAYGSASILPITYGYIRMMGAEGLTNASKIAILNANYLAERLKETYGVVYRGENG
                     RVGHELILECRNFKVAGITETDIAKRLMDYGFHAPTLSFPVHGTLMVEPTESESKAEL
                     DRFVEAMIQIYEEIMEIENGTADKTDNVLLNAPHPEYEMVSDEWKHLYSRKKAAYPTK
                     WTAENKFWINVARIDNAFGDRNLICSCPPTVEWQ"
     gene            6766..7878
                     /locus_tag="PLM04_05750"
     CDS             6766..7878
                     /locus_tag="PLM04_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HON02613.1"
                     /translation="MIDTLNLFGKTKIEVPKTEGIKYAGSKLKILPYIFNMISGLDVE
                     NILDGFSGTTRVSQALAKTGFKTTSNDISIWSATFAKAYLLNNRKPEYYLPIIEYLNS
                     LKGYVGWFSEYYGGTENNKEKRPFQLKNTMKLDAIRDEIDRMNLDEIEKSVALTSLIL
                     ALDSVDNTLGHYAAYLAEWSPRSYRDLKLKLPLLFKNNQQNEVIKGDIFETVKNHKFD
                     LAYFDPPYGSNNGKMPPSRVRYNSYYHIWTTVILNDKPKLFGKVNRRLDSKDEVAASV
                     FEEFRKDEQGHFIAMNAIKELIHCTNSKYILLSYSSGGRATKEELFDIINSEGKMIQA
                     IEINYKKNVMGQMRWTNEWINSDDKYMEYLFLMEKC"
     gene            7973..9295
                     /locus_tag="PLM04_05755"
     CDS             7973..9295
                     /locus_tag="PLM04_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HON02614.1"
                     /translation="MITSEPIIHVGILSSREIAFILNGIFIISDGKNFSGKQKAEWKN
                     GKILFDGETFDELNFQPHDESSTFDLLDVVIGIHFHWERKENQRFKGALKIIVENEQL
                     TVVNEIKAEDYLLSVISSEMSATSSLELLKAHAVVSRSWLFYPLQKDKKELSGRESVE
                     ENTENRHIKWYERGEHEHFDVCADDHCQRYQGITRATSDAVRHAVEATRGEVLMFEQQ
                     ICDARFSKCCGGVTETFDNCWAPEEHPYLSSIVDNDLLPQSFDLDLTMEENAQKWIRE
                     FPTAFCNTQDKNVLTQVLNNYDQETTDFYRWKVVYTQEELAEILRRRSGYDFGDILDL
                     IPLKRGKSGRIVELKIVGTKRTMIVGKELEIRKWLSDSHLYSSAFVVDKENYSQQIPG
                     KFILTGAGWGHGVGLCQIGAAVMGEKGYAYERILLHYFRGAELKRIYE"
     gene            9782..10912
                     /locus_tag="PLM04_05760"
     CDS             9782..10912
                     /locus_tag="PLM04_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HON02615.1"
                     /translation="MKKIPFLLLPCFVLFIACTHGSSHLAKEQPVDSLRLSHAEGFSI
                     RYFRNYKEVVVFNPFSHNAVYQRYYLVRDTDVSVPADGLKIKIPVRSLAATSVTHFEF
                     LNLLHVIDAVTGVCSSKIIYSPQINERLNKGLIADLGDAFNINLEKTVQLHPDLVMVS
                     GYNQNDPFAQRLLQAGIFPVYNNEWMEKSLLGRAEWIKFLAAFFDREKIADSLYLEIE
                     KEYQNTANRVKGIKFRPQIMSGSNFRGTWYMPGGRNFMAQLFSDAGGNYFYARDTTVG
                     SLPLSFEKVLKNFSESEVWLNCNYASLSELLQADPRHALFRPVKLQQVYNFNKRMLPS
                     GSNDFWESAVAHPDLLLKDVIFILHPELLPGYEPFYTQKLNP"
     gene            10920..11915
                     /locus_tag="PLM04_05765"
     CDS             10920..11915
                     /locus_tag="PLM04_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HON02616.1"
                     /translation="MYSRNHLLFFAFSLLFIFLFLADLAWGSVAIPAKDIFSVLCGQQ
                     SSSIYTEIIVNFRLPKAITAILAGASLSVAGLMMQTLFRNPLADPYILGVSSGASLGT
                     ALVVMASGFLPFSFVGNGWVLIISAIVGAALVLLIVLFVSFRVNDIVSLLIVGIMFGT
                     IAAALVSIIQNFSNPDAIKLFLMWTFGSLSAVTWNYLQILLPVVLVGIGIAFFLQKRL
                     DGLLLGENYARGLGIPISQTRSLIILATGLLAGGITAFTGPIAFVGVAVPHIARGIFQ
                     TSSHKILLPACMLVGAALLMICDIITQIPDYTLPVNTISALFGAPVIIWIILRRK"
     gene            11943..12932
                     /locus_tag="PLM04_05770"
     CDS             11943..12932
                     /locus_tag="PLM04_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON02617.1"
                     /translation="MSVLSTQHLSIGYRRKGTANVIQHDLNLKLESGKLVGLIGPNGS
                     GKTTLLRTIAGLLKPIDGNILIDDKEFLLLSQREKALNVSFVLTEKVDIPNADVFDMV
                     SFGRFPYNNWRGNLSKQDRTKVFQALQMVRMEKMMHRYINELSDGERQRVMIAKALAQ
                     DTPIILLDEPTAHLDVPNRVGIMLLLHKLAHKTGKMILLSTHELDLALQAADLIWLMS
                     KNGGIECGVPEDLVFSGSFNRTFASDYYFFNEANGNFSLNYPLTGKVSVSGNKMRVYW
                     TLRALARAGYEAVENAPLKIEVTDSAWILNQKEIFSVAEMLDELNNLNGNTSL"
     gene            13319..13516
                     /locus_tag="PLM04_05775"
     CDS             13319..13516
                     /locus_tag="PLM04_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HON02618.1"
                     /translation="MSKKLTRSSNQMIAGVCAGIAEYFGWNVTLVRVIYVLLSIFSAG
                     FPGTMVYIILWIVMPRPEKFD"
     gene            13590..15608
                     /gene="uvrB"
                     /locus_tag="PLM04_05780"
     CDS             13590..15608
                     /gene="uvrB"
                     /locus_tag="PLM04_05780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HON02619.1"
                     /translation="MNFKLESPYQPTGDQPEAIQQLVEGINAGIPYQTLLGVTGSGKT
                     FTIANVIEQVQRPTLVLSHNKTLAAQLYSEFKSFFPHNAVEYFVSYYDYYQPEAYLPL
                     TDTYIEKDLSINQEIEKLRLSATSSLLSGRRDVVVVSSVSCLYGIGNPDDFSKNVIEI
                     RKGQHLVRNAFLRNLVNSLYTRNEIELNRGNFRVKGDTVDIFLAYTDFILRIVFWGDE
                     IDEILTVDPVNYDIIDSLDAYKIYPASIFVTTKERIAVAINEIEKDLQLQVDYFRSIG
                     KDLEAKRIYERVRYDIEMIKELGYCSGIENYSRYFDGRPPGSRPFCLLDYFPKDFLLV
                     IDESHVTIPQIRAMYGGDASRKQNLVDFGFRLPAAKDNRPLRFEEFEQLTPQTIYVSA
                     TPADYELVKSEGIVVDQLIRPTGLLDPVIEVRPSLNQIDDLLEEIAQRTENNERVLVT
                     TLTKRMAEELTNYLSKMGVKCNYIHSDVETLDRVQIMEDLRRGVYDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSHRSLTQTAGRAARNLNGKVIMYADQITESMEKTINET
                     NRRREKQLRYNEAHHITPTAIVKGERNPLSKAEPYAYVEPEKSTDIAADPVVRYMSVP
                     ALEKAIAKTRKAMQEAAKKLEFLEAAQLRDELMKLEELLKKKMFEAKN"
     gene            15787..16407
                     /locus_tag="PLM04_05785"
     CDS             15787..16407
                     /locus_tag="PLM04_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HON02620.1"
                     /translation="MLEIIPKGILIGLCISVPVGPIGMLVIQRTLNRGRKYGVATGLG
                     ASMSDLFYTIITLFFLSFVISFIEAHRFVIELIGSLVVICFGYFIYKNNPSVQPKPNE
                     TVQHNVYRDFISSFVLTFSNPLILFILIALFARIGFIDSETSFFQTIIGIISILIGAT
                     IWWLTLTFIVSHFRSKIGMHQLKAINRITGGLIALIGCIGLVSSLI"
BASE COUNT         5350 a         3147 c         3439 g         5421 t
ORIGIN      
        1 ttcagccaaa ttcaaggatt gacattctca tttattactc gatagatttc aaaaaagacc
       61 gtaagaaaaa cttgcggtct tttttgttga aaaaatgaaa attacatttg taatttcctt
      121 aaaattttct atatcccatt tatactgaac gggtaatcaa aaaaatttag gtagctccaa
      181 aaacaagatt ttgccgaata ataaaacttt gtgaaataat caattgaaat tttctaaatt
      241 gaggaacata ttcaaatcgt cctccacgct gccaatagga gcaattccaa aattatctac
      301 caacactttg gcaacatttg gtgaaagaaa tgccggcaat gtgggtccca aatggatatt
      361 tttcactccc agataaagga gcgcaagaag cacaattaca gctttctgtt cgtaccatgc
      421 aatgttatag actatgggca attcattgat actgtttaaa ttaaaaattt ctttcagttt
      481 cagggctatc agtgccagcg aataactgtc gttacactgg ccggcatcga gcactcgcgg
      541 aatgccattg atttctccca atgcgagttt gttatagcga tattttgcac agccagccgt
      601 aagaataacg gtatcttttg gcaatttttc cgcaaattcc gtataataat tgcgactttt
      661 taatcttccg tcacaaccgg ccattacaaa aaatttccta atttgtccgg atttcactgc
      721 ctcgataatc tgatccgaca gagcaaaaaa ttgctcgtga gcaaaaccgc ccacaatttc
      781 tcctttttca atctgtatgg gagcggcaca ttgtttggca tgttcaatca acgacgaaaa
      841 atcctttgcc tttccttcag gtgcttcggc aatatgtttg aagcccggaa aacctgccgc
      901 tccggtagta taaaccttgt cggcataagt ggagttgctt ctcggtggaa caatacagtt
      961 ggttgtaaaa agaatcactc cgttaaatgt ttcaaaatcc tgtgtttggt tccaccatgc
     1021 tccgccataa tttccgacaa aatggtcgta ttttttaaat gccggatagt agtgtgccgg
     1081 caacatttct ccatgtgtat aaacgtcaac gcctgttcct tcggtttgtt tcagcagctc
     1141 ttccatatct ttgaggtcat gtccactgat taagataccc ggcttttccc gagtttgcaa
     1201 actgactttt gtaacttgtg gatgtccata agcagttgta ttggctttat cgagcagctc
     1261 catgactttg actccgaaac gtccggtttc caaaaccaaa tttatcagct cttccatact
     1321 caatgtatcg tctgtggttt ttacgagtgt ttcttctacg aaagcattta tttcgccgtc
     1381 ggtgaaacgg agattcatgg catgtttggc ataagcagcc attcctttca gtccataaat
     1441 tatcagttcg cgcaaagagc ggacgtcttc attttcggta ctcaacacgc caactttgct
     1501 tgccatattt tccatatctg cttcggtatc agcaaaccac tccgtgagtt cggacgccgg
     1561 caatgttatc ccatgaattg ataaatcttg ttttaattga gaacgcagat cgaatccttc
     1621 acgaatttgt tcaacaaatc gctgtttatc gaaattgaca ttggtaaccg tcataaaaag
     1681 gccgtcgatg ataaaacgac tcacaagcgg actttcaact cccactttgc gcaaagcggt
     1741 tgtgtaattt gataatcctt tcagctgata aatcaacaaa tcctgcaaag ccgccacatc
     1801 agaagtttta ccacaaacac ctttcaattc gcagccggtt cctttagaag tttcctgaca
     1861 ctgataacaa aacatattca tttttttcac aattaatttt aaaatctcaa aatctatttt
     1921 tatttacttg tttttcaatc ttttatcatt accaacatca ttttaaaacg ttctgtggca
     1981 ttcaccgcat gtggaacacc ggcaggtaga atgatacttt ctccggaaat taattcaaac
     2041 ggctgccccg aaatggtaat cgttccttta ccatcgagag ctaccaccaa tgcattgaat
     2101 ggtgctgcat gttcacttaa aaattctccc ttgtcgaaag caaacagtgt aatgtttccc
     2161 tgacgatttc gaacaatgat tttgcttacc accgagccat cggcatagtt gacctgattg
     2221 gccaatacaa acttttcgcc tgcaggaatt gttttgcttt cctgcctgtt gttagcttct
     2281 gaattttcca tcatgttttg aatttttagt tatgtaattt tataaattgt ttttgaaatt
     2341 gaagcacaat actttatatt tgtacactgt acaagattga agttttttga ggttgcaaag
     2401 ttagagagtt taaaacaatt aaaatgtaac acttgttaca gatgaaaaaa attgattttt
     2461 catgaaaagc attgatccag aatggaagag ttacggcagt attgatgatg gagaaagtgg
     2521 tttccctgcg gatttaaaat acagcattaa gccataccga aaaaacgaaa ttatgtttcg
     2581 tcagggaagt atttgcgatg cattgtacat tttgacctcg ggttcggtga aaacagaaat
     2641 gattaccgaa accggcaata ttatgggaat agaactgata aaagcgcctc gtccgttggc
     2701 tccggctttt ttattttccg acaacaaccg ttttcccgta gatgttactg cactcgaaga
     2761 ctgcgaaatc ctacgtattc ccaaagaaga agtaatgcgt ctgatggcga tacagcccga
     2821 ttttatgcag cagtttttaa aacacaatgc caaccgagca gagtttttag ccaatcgact
     2881 tcagttgctt tccattaaaa ccataaaggg acgcattgct cattttttat tggaacaaat
     2941 ggccgaatac ggaaaaactt tcactatcaa ccgaaacaga accgaattgg ccgagttttt
     3001 tggcgtggca cgtccttcat tggccagaag tctttcggaa atggtcgaag acaacatcat
     3061 taaaatcaac aaaaaacagt ttactgtaat tgatgagcgc aagttaaaag atttgctcct
     3121 ttaatgttaa aaacttaata aattgcgaca ataaaaattt attgccactc aacagttgga
     3181 ggacaggaac aaattaaatt cctatcacca aaagcattat caatacgtgc gacatttatc
     3241 caaaatttgt tttcagcagt ccatttcgta ggataagcgg ccttttttct gctgtaaaga
     3301 tgtttccact catccgacac catttcatat tccggatgag gtgcattcaa taaaacattg
     3361 tccgttttgt cggctgtccc attttcaatt tccataattt cctcataaat ttgaatcata
     3421 gcttccacaa acctgtcgag ttctgctttt gattcacttt cggttggctc aaccatcaac
     3481 gtaccgtgaa caggaaaaga caaagtagga gcatgaaatc cataatccat caaacgtttg
     3541 gctatatcgg tttccgtaat accggcaact ttaaaatttc gacactccaa aatcaattcg
     3601 tgccccacgc gaccattttc gccccgatac acgactccgt aagtttcctt taaacgttcg
     3661 gccaggtagt tggcatttaa aatagcaatt ttagaagcat ttgtcaaacc ttcagctccc
     3721 atcattcgta tataaccata cgtgatgggc aaaatactgg cactgccgta agctgcggct
     3781 gaaacagtat tctgaccgga tgttttaccc ggcaaaaaag gtgccaaatg agcagcagca
     3841 caaacgggtc caacaccggg accgcctcca ccgtgaggaa tggcaaaagt tttatgtaaa
     3901 ttcaaatgac aaatatccgc accaattttt gccggatgag ttaatccgac ctgagcattc
     3961 atattggctc catccatata aacttgtcct ccgtttgcat gaataatttg gcacatttcc
     4021 ccaatgtgag cttcaaaaat gccatgtgta gaaggataag ttaccataca acccaacaat
     4081 cgcgtacgat aagtttctgc tttcactttc cagtcgttga gatcaatatt cccattttca
     4141 tcgcaatcga ccacaactat ttcaagcccc gcctgatgag ctgaagccgg attggtgcca
     4201 tgagccgatg ccggaattaa aacaacgttt cgttctgttt caccacgcga ccgataaaag
     4261 gctttgatag tcatcaagcc ggcaaactct ccggctgcac cggaattggg ctgcaaactg
     4321 caagcatcaa aaccggtaat aacacacaaa tatttttcca attcgccaat tagttcaatg
     4381 taacctttca cttgatcgga cggagccaaa ggatgaatct tcaaaaattc tgctttgctc
     4441 aacggtagca tttctgaagc tgccgtgagt ttcatggtgc acgaccccaa tgaaatcatg
     4501 gaatgagtca acgatatatc ttttcgttcc agtttcttga tataacgcat catttccatt
     4561 tcggtatggt agcggtgaaa aatttcctga gtcagataag ccgactttcg tctgattttt
     4621 tcatccggta tttcaaactc cgacaagtcg gttggattga agacaatcgg ttttttatga
     4681 acggcttccg aaaaaatatt tatcaatctt tgtatatcgg gtgcttcggt agtttcattt
     4741 atactcaaac ctacttctcc ggtttcaaaa taataaaaat taactttgtg ggagagcgca
     4801 atgtttcgaa attcttcttg agaaacattt tcgggcaaaa ctattttcaa agtatcgaaa
     4861 aaaagtttat tcaactgacg ataaccgtaa gttggcagca ttgcattgat ataagctgct
     4921 atggcatgaa tgtttttcgc tatatttcta attccctccg gcccgtgaaa aacggtataa
     4981 aaacccgcca tcgaagccaa aagagcttgt gcggtacaaa tgttagaagt tgccttttcg
     5041 cgtttgatgt gctgctcacg ggtttgaagt gccatacgca aagccggttt cccgttttta
     5101 tccttgctaa tgccaatgat gcgacccggc atatcgcgct taaattcatc gcgtgtagca
     5161 aaataagccg ccgaaggacc tccaaacccc ataggaattc cccaacgttg agtagaacca
     5221 aaaacaatat cggcgcccca ttctcccggt ggaacaagta aagccaaact taaaatgtcg
     5281 gcagccacag caactttaca acccactgta tgtgcttgtg atacaaatga aaaataatgc
     5341 tctacattcc catccgcatt aggatattga agaatcgctc caaaatgttt ttctgaaaat
     5401 tgtacggatt tataatcccc aatttcgagt tgtattccct gtgcttgtgt ccgcgtttgt
     5461 aaaacagcca aagttgaagt aaaaatattt tcatcgacaa aaagcaaatt tgctccattt
     5521 ttcatttgtt ccctcgaacg caaattatac atcatggtag ctgcttctgc tgcggcagtt
     5581 gcttcatcga gcaaagaagc atttgccaat ggaagaccgg taaattcaca aacggcggtc
     5641 tgaaaattca gcaaagcttc caatcttcct tgagaaattt cggcttgata gggagtgtag
     5701 gaagtgtacc atgaaggatt ttcaaaaaca ttccgtaaaa ttacggccgg tgtacaggtg
     5761 tcataccatc ctctgccaat ataggaagta aaaattttat ttttttctgc aatttcagaa
     5821 atatgctgag catactgatt ttcagtcaga gcagaaggga gattcaattt ttcgttcaaa
     5881 cgaatggaga gaggaatagt ttgagtgata agttcatcta aactttcagc ccctacaact
     5941 tccaacattt cttgaatttc atcagacgaa acgccaatgt gtcgaaaaga gagaaaatct
     6001 ttcatttgat tatatattac tgatttttag ttaattataa aaatttgaat gatcgaatat
     6061 taaaaaataa agcggctaaa atttcaagtt gtaaagttac agcaaaaaaa tcaggaaaga
     6121 aaattcaatt ttttaacaaa atattgtttg agtccactcc gaaaagtttt tcaaagagaa
     6181 ttagttttta aagacattgg aagtacttga ccatttgtag agaaggtttg tgccgttggt
     6241 gcttatgaca gtgagaaaaa tcaagaattt gcaacgacaa acaacaaaga acttatttca
     6301 agtggtatac agggagccaa aggaagatat gaactttcag ttgattcaca aaatcctgac
     6361 aatttaatag taaccaatac ccagacagga gaaattataa aagcacaaaa ataaaaacaa
     6421 aaagaagagt ggtaatctac aatggcgtat caaaacagag catggcaact atcgttattt
     6481 taaccttgag caggtgcaaa tttttcttgc ttcttttcaa aaaaaaatct cattttttat
     6541 cgtttttgaa aaaaattagg ttttaaaaaa aaggactcaa tacttaattt aggtcagctt
     6601 tacaaataga caataattca atacgtttta ttaaatttgc aaatcaaact tcgattataa
     6661 gtaaaaaaac atggattctt agaatgattt taaacaactt ttcaaacttt tggcagacaa
     6721 aaaggtactg agttgttaat tttctaagca atattcacga tgataatgat tgatacatta
     6781 aatttatttg gtaaaaccaa aatagaagtt ccaaaaactg aaggaattaa atatgctggt
     6841 tcaaaactaa aaattcttcc atatattttt aatatgattt ctggcttaga tgtagaaaat
     6901 atattagatg gattcagtgg aacaacaaga gtatctcaag cacttgccaa aacgggtttt
     6961 aaaactactt caaacgatat atctatatgg tcagcaactt ttgcaaaagc atatttgcta
     7021 aataatagaa agcctgaata ctatttgcct attattgagt atttaaattc attaaaaggt
     7081 tatgtaggtt ggttctctga gtattatgga ggcactgaaa ataataaaga aaaaaggcct
     7141 tttcaactta aaaatacaat gaaactggat gcaattagag atgagattga tagaatgaat
     7201 ttagatgaaa tagaaaagtc tgtagctcta acaagtttaa ttctcgctct tgattcagta
     7261 gacaacacat taggccatta tgctgcatat ttagcagaat ggtctcctag atcatatcgt
     7321 gatttaaaat taaaattacc gttattattt aaaaataatc agcaaaatga agtaataaaa
     7381 ggagatattt ttgaaacagt aaaaaatcat aaatttgatc ttgcttattt tgatcctcct
     7441 tatggttcaa ataatggaaa aatgccacca agcagagtta gatacaattc atattatcat
     7501 atctggacaa ctgttatatt gaatgacaag ccaaaacttt ttggaaaggt taatagaaga
     7561 ctggattcaa aggatgaggt agcagcgtca gttttcgaag aatttagaaa agatgaacaa
     7621 ggtcatttta ttgcaatgaa tgcgataaaa gaattgattc attgtacaaa ctctaaatat
     7681 attcttttat cgtatagttc gggtggtaga gctacaaaag aagaactttt tgatataata
     7741 aattctgaag gtaaaatgat tcaagcaatt gagataaatt ataagaaaaa tgttatgggt
     7801 caaatgagat ggacaaatga atggataaat agtgatgata aatatatgga atacctcttt
     7861 ttaatggaaa aatgttgatt ccactcactt caaacttatt gttattctta tattgagaaa
     7921 aattttttga aagaattgaa agcctaaaat agattaaaaa caaatcatag aaatgattac
     7981 ctcagaacca attattcatg tggggatatt aagttcccgg gaaatagcat ttattctaaa
     8041 tggcattttt attatttccg atggaaaaaa tttttccggg aagcaaaaag ccgaatggaa
     8101 aaatggaaaa atactttttg atggcgaaac ttttgatgaa ttgaattttc aaccgcatga
     8161 cgaatcgtca acatttgatt tactagacgt agtgatcggg attcattttc attgggaaag
     8221 aaaagaaaat cagcgattta aaggagcttt gaaaattatt gtggaaaatg agcaattaac
     8281 agttgtcaac gaaataaagg ccgaagatta tctgctcagt gtaatttcca gtgaaatgag
     8341 tgctacctcc tcacttgaac tgctgaaagc ccatgccgtt gtttccagaa gctggctgtt
     8401 ttatcctctg caaaaagaca aaaaagagct ttccggtcgg gaatcggtcg aagaaaacac
     8461 ggaaaatcga catatcaaat ggtacgaaag aggcgaacac gaacattttg atgtgtgtgc
     8521 cgacgatcat tgtcagcgtt atcaaggaat tacaagagca acttcggatg cggttcgaca
     8581 tgcagtagaa gccacgcgtg gcgaagtgct gatgtttgag caacaaatat gtgatgctcg
     8641 tttttccaaa tgctgtggcg gagttaccga aacttttgac aattgctggg cgcccgaaga
     8701 gcatccttat ttatccagta ttgtggacaa tgatttatta cctcaaagtt ttgatttgga
     8761 tttaacgatg gaagaaaatg cccaaaaatg gattcgtgaa tttccgacag ctttttgcaa
     8821 tacgcaggac aaaaatgttc tcacacaggt gttgaataat tacgatcagg aaacaacaga
     8881 tttttatcgc tggaaagttg tttacaccca ggaagaactt gccgaaattt tgcgacgtcg
     8941 ttcaggatat gatttcggag atattttaga cttgattcct ttgaaacgag gtaaatcggg
     9001 ccgaattgtg gagttaaaaa ttgtgggaac caaacgaacg atgatagtag gtaaagagtt
     9061 ggaaattaga aaatggctgt ccgatagcca tttgtatagc tcggcttttg tagttgacaa
     9121 ggaaaattat tcccaacaaa ttccaggaaa atttattttg accggtgcgg gttggggaca
     9181 tggcgtagga ctttgtcaaa ttggtgctgc cgttatgggc gaaaaaggtt atgcctacga
     9241 acgtattttg ctgcattatt ttcgaggcgc tgagttgaaa aggatttatg aatgaagaat
     9301 agtattttta gcattttctt gattccattt catcagaata taatttattt tttaatggtc
     9361 tgatggaacc cacatgtcta aatgttatat tcattcgtgt ttgtgtatgt aatacatgtg
     9421 ggtttcttaa ttgaaaataa taaataaatt ttgagtccat atttttaata ttttgtataa
     9481 atttacttaa ttgattaata atcaattgtt taaaaaaaat aacataatct gtataaatgg
     9541 ttgattgtaa gtataatttc aatattgatg tcagagaata ttcgagaata aaaaattttt
     9601 cactttttta gtttactcta tttcaatagt ttgataaagt aatatacttt cattcattgg
     9661 tcattttcag tttttcaaac tatctttgca agcggttaat ccttcggaaa agttttttgg
     9721 aaaaaatgaa tttcaagaca atgaaaatta tcaaaggctt ttccgataat ttggtaaaac
     9781 aatgaaaaaa attccgtttt tgttgcttcc ctgttttgtt ttgtttatcg catgtacaca
     9841 cggaagttcg catctggcaa aagagcagcc ggtggattcg cttcgacttt ctcatgccga
     9901 aggtttttcc attcgttatt ttcgaaatta taaagaagtg gttgttttta atcctttttc
     9961 gcacaatgct gtttatcaac gttattattt ggttcgggat actgatgtgt ctgtgccggc
    10021 ggatggcttg aaaatcaaaa ttcccgttcg ttcgttggca gcaacatcag ttacccactt
    10081 tgaatttttg aatttgcttc atgtgattga cgctgtaacg ggtgtctgtt cttcaaaaat
    10141 tatctacagt ccgcaaatta atgagcgtct caacaaaggc ttgatagccg atttgggaga
    10201 tgcattcaat atcaatctcg agaaaacggt tcagttgcat cccgatttgg tcatggtaag
    10261 cggctacaat caaaacgacc cttttgcaca gcggttgctt caggccggaa tttttccggt
    10321 ttacaacaac gaatggatgg aaaaatcgct gttggggcgc gccgaatgga taaaattctt
    10381 ggcggctttt tttgacagag aaaaaattgc cgacagtctt tatctggaaa ttgaaaagga
    10441 atatcaaaat actgccaaca gggtaaaagg gataaaattt cgtccgcaaa taatgtccgg
    10501 ttccaatttt cgtggcactt ggtacatgcc cggaggacga aattttatgg cacaactttt
    10561 ttcggatgcc ggtggaaatt atttttatgc ccgcgataca accgtaggaa gtttaccgct
    10621 cagttttgaa aaagtactga aaaatttttc tgaatccgaa gtttggctaa actgcaatta
    10681 tgcttcattg tccgaattgt tgcaggccga tccgagacat gctcttttcc gtcccgtaaa
    10741 gttgcaacag gtgtataatt tcaataaacg catgttgcca tctggctcca acgatttttg
    10801 ggaaagtgcg gtagctcatc ccgatttact tttgaaagat gtgattttta ttttgcatcc
    10861 cgaattgctg cccggttacg aaccatttta cactcaaaaa ttaaatccgt aaggttttta
    10921 tgtattcgag aaaccatttg cttttttttg ctttttcgtt gctgtttatt tttttgtttc
    10981 tggccgattt ggcatgggga agtgtagcta ttccggcaaa agatattttt tcagtgctct
    11041 gcgggcaaca gtcttcctca atttatacgg aaataattgt taatttccgc ttgccaaagg
    11101 ccattacggc catacttgcc ggcgcttcgc tttcggtagc cggccttatg atgcagacgc
    11161 tttttcggaa ccctttggcc gatccgtata ttttgggcgt cagttcgggc gcaagtttgg
    11221 gcacggcgct ggtggtgatg gcttccggtt ttttaccgtt ttcctttgtc ggaaatggtt
    11281 gggtactgat aatttcggct attgttggcg ctgcattggt gctgttgatt gttctttttg
    11341 tttcctttcg cgtcaacgat attgtttcgc tgctcattgt cggtattatg tttggaacca
    11401 ttgcagctgc tttggtgtcg attattcaga atttcagcaa tcccgatgcc ataaagctgt
    11461 ttttgatgtg gacattcgga agtttaagcg ccgttacgtg gaattatttg caaattttgt
    11521 tgcctgttgt tttggtaggc ataggcatcg ctttttttct gcaaaaaagg ctcgacggac
    11581 ttttattggg cgaaaattat gcacggggtt tgggtattcc catttcacaa acccgatcgc
    11641 tgattattct ggctaccgga ttactggcag gaggaatcac tgcttttact gggcccattg
    11701 cattcgtagg ggtggcagtt ccgcatatag cacgaggaat ttttcaaact tcatcacaca
    11761 aaattttatt gccggcatgt atgctggtcg gtgcagcatt gttgatgatt tgcgatatca
    11821 ttactcaaat tcccgattat acgttgccgg tgaataccat cagtgcctta tttggtgcgc
    11881 cggtaattat ttggatcatt ttgagaagaa aataagtcat gaagaaatta taagtcaata
    11941 atatgtcggt tttatccaca caacatttat cgattggtta ccggagaaaa ggaacggcta
    12001 acgtaattca acatgacttg aatttaaagc tggaatcagg gaaactggta ggtttgattg
    12061 gcccgaacgg aagtggaaaa actacgcttt tacgaactat tgcaggattg ttgaaaccca
    12121 tcgacggaaa tattttgata gatgacaaag agtttctttt gctttcgcaa cgtgaaaaag
    12181 cattgaatgt ttcttttgta cttaccgaaa aagtagatat tcccaatgcc gatgttttcg
    12241 atatggtatc ctttgggcgt tttccctata ataattggcg gggaaatctt tccaagcagg
    12301 acagaacgaa agtttttcag gcgctgcaaa tggtcaggat ggaaaaaatg atgcatcgat
    12361 atatcaatga actttccgac ggagaacgtc aaagagtcat gatagccaaa gcgttggctc
    12421 aggatacgcc cattatttta ctcgatgaac caactgccca tctcgatgtt cccaatcgtg
    12481 tgggaattat gttgctgctc cataaattgg ctcataaaac cggtaaaatg attctgcttt
    12541 cgacacacga actggatttg gcgttacagg ctgccgacct gatttggctg atgagcaaaa
    12601 acggaggaat tgaatgcgga gtccccgaag atttggtttt tagcgggagt ttcaatcgaa
    12661 cttttgccag cgattattat tttttcaatg aagccaatgg taacttttcc ctgaattatc
    12721 ctttgacagg aaaggtgagc gtatcgggca acaaaatgcg tgtatattgg actttgcggg
    12781 ctttggcacg tgcaggatat gaagcggtgg aaaatgctcc gttgaaaata gaagttaccg
    12841 acagtgcatg gatactgaat caaaaggaaa ttttttcggt ggccgaaatg ttggatgaat
    12901 tgaataattt gaatggcaac acttcattat aatttatttt gacttcttag tcagtttaac
    12961 gtaaatattc actaccgata ttcctatata tcgattaact gcgcctgttt gtcccaactt
    13021 tacaaggtag ttctttacta taaagataac cataatcatc aattccttca actaaaataa
    13081 ttgattttta atttattaag tgaatttttc accgaagtat tatgaatgaa gttgtcgata
    13141 ttaaattctt tttctttaga ttgactaata tctatgctta gacacatatt tttaaatggg
    13201 gacccataaa tttggttaat tggattcttg aaaaaacagg atgagaaata ggttatttgt
    13261 cggttttttt tctttatttt gtattgaatt acattaccaa attaattttt aaaaggctat
    13321 gagcaaaaaa ttgacgcgtt cgagcaatca aatgatagcc ggagtttgtg ccggaatagc
    13381 agaatatttt ggttggaatg taactttagt aagagttatt tatgttttgc tttccatctt
    13441 ttctgcagga ttccccggta ctatggttta cattattctt tggattgtca tgcctcgccc
    13501 cgagaaattc gattaaataa ccgaaagaac aaaagtcgtt gtgaaaacga cttggaaaca
    13561 aacctataat ttacactttt ttaaacgtta tgaattttaa gctcgaatcg ccctatcagc
    13621 ctaccggtga tcagccggaa gccattcagc aactggtaga gggaatcaat gccggtattc
    13681 cgtatcaaac tttgttgggt gttaccggat cggggaaaac ctttaccata gccaatgtta
    13741 ttgagcaggt tcagcgtccg acgctggtac tgagtcacaa caaaacgctt gctgcccagt
    13801 tgtacagcga attcaaatct tttttcccac acaatgccgt agaatatttt gtttcctatt
    13861 acgattatta tcagcctgaa gcgtatttgc cattgaccga tacgtatatc gaaaaagatt
    13921 tgtccatcaa tcaggaaatc gaaaaactga gactctcggc cacctcttcg cttctttcgg
    13981 gacgcaggga tgttgtggtg gtatcatcgg tttcgtgtct gtatggcatt ggtaatcccg
    14041 acgatttttc gaaaaatgta attgaaattc gaaaaggaca gcatttggtg aggaatgctt
    14101 ttttaagaaa tttggtcaac agcctttata cccgtaacga aattgaactg aatcgtggaa
    14161 attttcgcgt aaagggcgac actgtggata tttttcttgc ctataccgat tttattttgc
    14221 gtatcgtttt ttggggagac gaaattgatg agatactgac agttgatcca gtcaattacg
    14281 acattattga ttctcttgat gcctataaaa tatatccggc cagcattttt gtaaccacaa
    14341 aggaacgaat tgctgtggcc ataaatgaaa tcgaaaaaga tttgcagttg caggtggatt
    14401 attttcgctc cattggaaaa gatctcgaag ccaaaagaat ttatgaaaga gtacgttacg
    14461 atatcgaaat gataaaagaa cttggttatt gttcgggaat cgaaaactat tcgcgatatt
    14521 tcgacggtcg tccgccggga agccgtcctt tctgtctgtt ggattatttt ccgaaagatt
    14581 ttctgttggt cattgatgaa agtcatgtta ccattcctca aataagagcc atgtacgggg
    14641 gcgatgcttc acgtaaacag aatttggtcg atttcggatt ccgattgccg gctgccaaag
    14701 acaatcgtcc cttgcgtttt gaagaatttg aacagcttac acctcaaact atttatgtga
    14761 gtgcaacccc tgctgattat gagttagtaa aatcggaagg tattgtggtt gatcagctta
    14821 tacgtcctac cggactgctc gatcccgtta ttgaggttcg tccgagttta aatcagattg
    14881 acgatttgct ggaagaaatt gcccagcgaa ccgaaaacaa cgagcgggtg ctggtaacta
    14941 cccttaccaa aagaatggct gaagaactga ccaattatct atcaaaaatg ggagtgaaat
    15001 gcaattatat tcattctgat gtcgaaacgc tggatcgggt tcaaatcatg gaagatttgc
    15061 gcagaggagt gtatgatgtg ttggtcggtg tcaacctgtt gcgcgaggga ttggatttac
    15121 cggaagtttc actggtggct attttggatg ccgataagga aggatttttg cgttctcatc
    15181 gttcgttgac gcaaacggca gggcgagctg cacgaaattt gaatgggaaa gtcatcatgt
    15241 atgccgatca gatcaccgaa agcatggaaa agaccataaa tgaaaccaat cgccgcagag
    15301 agaagcaact tcgatacaat gaagcgcatc atattacgcc aactgctatt gtgaaaggcg
    15361 aaagaaatcc gctgtcgaaa gcagaaccat acgcgtatgt ggaaccggaa aagtcgaccg
    15421 atattgcagc cgatccggtg gtgcgatata tgtccgtgcc ggcacttgaa aaagccatag
    15481 caaaaaccag aaaagcgatg caggaagctg ccaagaaact cgaatttctg gaagcggctc
    15541 aactcaggga cgaattgatg aaactggaag aattgttgaa aaagaaaatg ttcgaggcga
    15601 agaattgaaa agaaaatgtt gctttaaaag tttcaaaaaa tcaatttcat aagtcgaaac
    15661 aaaaggacaa ttcattcaaa aaacaccaaa caagccctaa aaattgttga attattgcat
    15721 taaatttagt tttctgatga tagaaaaatt atctttgtat cgctaattca ccagaaaacc
    15781 tataatatgc tggaaatcat tccgaaaggt attctaattg gtttgtgtat ttctgttccg
    15841 gtaggtccca ttggtatgct ggttattcag cgtaccttga atagaggaag aaaatacgga
    15901 gtggccaccg gtttgggtgc gagcatgtcc gatttatttt ataccatcat cactttattc
    15961 tttttgagtt ttgtgatcag ttttattgag gctcatcgtt ttgtcataga attgatagga
    16021 agtttggttg tgatttgttt tggatatttc atatataaaa ataatccgtc agtacaaccc
    16081 aaaccaaacg agacagttca gcacaatgta taccgcgact ttatcagttc tttcgtattg
    16141 actttctcta atcccctcat tttgtttatt ttgattgcat tatttgccag aattggattt
    16201 attgactcag aaacatcttt ttttcaaacc attattggta tcatatctat tttaattggt
    16261 gctactattt ggtggttaac tcttactttt attgtcagcc atttcaggtc aaaaattgga
    16321 atgcaccagt tgaaagccat aaatcgaata acaggtggac tgattgcttt gattggatgt
    16381 attggacttg tttccagttt gatataaaaa ttcacccttc tttcttcgca gaaaaaaggg
    16441 tgtacttcac tatgggtgaa tcatttaacc cagaatccgc aatagggaaa aagttttaat
    16501 tttgtaggaa agtctaatgc gtagcaatag aaaaaaagac gtgtatgaac tttggtatct
    16561 cctttacaaa caaagagata acaccatgtg gtggaatgat ttttttgtaa caaatgttgg
    16621 aaaaaccacg tagaatcgtt attgttcgtc aaaaattaaa ggaaagcact ttcgtctgtt
    16681 tgaggaaaaa gaaatttaca gaaattatcg ttattctgct tacgtaacca acatgaaact
    16741 ttaagcgcct gacatatgaa gaaatacaga ggaagagttg atgctgaaaa cagaataaag
    16801 gaattaaaat atgactttgg ctttgacagt tttaatttaa aaaacttttt tgcaaggaaa
    16861 gccgttttaa ttttgtgatg atagcctata atcttatgtc gcagtttaga atgttcattt
    16921 tgcaggaaaa aacgcaaaaa aatttatcta cacacttaga ttttagtaca tgacaaaaat
    16981 tgagacattt gttttataca agacatataa gccaagctct tggggttggg gttggggttg
    17041 accttgtccg gtgagccgaa aaacaagtga gaaaagcgtt taaaaatcgt ccttgtttga
    17101 gttccgacgt atttcgtcgg aatgagtttg ggcgatttca gcttttcgag cgtaagtttt
    17161 tcgagtgaag tggacacagt cttgaaagca gcgtttaagt gaaaacaaaa tcaaatttat
    17221 ttgatttatg ttgagcgtag ctgcttcaac taagttaatt tttttgctta cttttttgtt
    17281 tcaagacaaa aaagtaagtg gagtttgggg caaagccccg ttattaaata tctgaaaata
    17341 tagaatataa atgtttc
//
LOCUS       DAOTAX010000044        16684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2873_length_16684_cov_30.260569, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000044 DAOTAX010000000
VERSION     DAOTAX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16684
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_16684_cov_30.260569"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1189
                     /locus_tag="PLM04_05790"
     CDS             167..1189
                     /locus_tag="PLM04_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON02621.1"
                     /translation="MQPLIEAVDKEVLKRELTPEKFLRPTNKSDNEIYVITAHNSPNV
                     MLEIGRLREMAFRSWGGGTGKEIDVDAYDYSEKPYYQLIVWDPEENEIVGGYRFLFGK
                     DVPLNEKGEPEFVMSHLFKYSKKFINEYLPYTIELGRAFIQPAYQTSQKGSKSIFALD
                     NLWDGLGALIHLAKEARYFIGKVTMYHAYPAAARELIYEYMFRFFPDKEKLILPRNPV
                     TISNNIKILSNKLFNEKDKEANYKILQKMVRKENVNIPPLFNAYIGLTNTLRMFGSAI
                     DPNFSSVYETGIMLTMSDIVETKRKRYIEPYIEYLKNVFSNGTFSKINAYESHCRNIY
                     NFFQFE"
     gene            complement(1202..3001)
                     /locus_tag="PLM04_05795"
     CDS             complement(1202..3001)
                     /locus_tag="PLM04_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HON02622.1"
                     /translation="MCGIAMIRLLKPLDYYQKKYGTWEYGLQKMYLLMEKQHNRGQEG
                     AGIATVKLDMPPGEEYISRKRIEGKNAIQEIFVDIDKNHSLAPEDYSSDAAKAKMFCP
                     FAGELYLGHLRYSTSGKSGLSYIHPFLRRHNWKSRTLVLAGNFNLTNVDEIFQSLTAQ
                     GQHPRDKSDTFLMLESLGDKLDREVQREYEIIKQRYSNDQVITRKIEENLDIASFIRK
                     SEHIWDGGYVICGMLGHGDAFLFRDPKGIRTAFYYADDEIIVAASERPVIQTAINVKA
                     EQVHELKPGEALIIKKNGSMHFETIREADPEPKPCSFERIYFSRGSDKDIYQERKKLG
                     GFLSEAILKAVDQDIDNTVFSFIPNTAEVAFYGMTDGIRTLTGKVPRIEKVLLKDIKL
                     RTFISQNKERNDLAAHVYDITYGSIRPEKKDNLVVIDDSIVRGTTLKQSILNILNRLE
                     PKKIVIVSSSPQVRFPDYYGIDMARMNEFCAFRAAVQLLKDSGRQKIMDDIYEKCKQQ
                     ENWPKEKVTNYVKEIYAPFTQEEISSQIAKMLTPDEVDCPVELVYQSIDNLHKACPQH
                     KGDWYFTGDYPTPGGNRMVNKAFIDYYEGKDKN"
     gene            complement(3057..4898)
                     /gene="glmS"
                     /locus_tag="PLM04_05800"
     CDS             complement(3057..4898)
                     /gene="glmS"
                     /locus_tag="PLM04_05800"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HON02623.1"
                     /translation="MCGIVGYMGYREAYPILIKGLQRLEYRGYDSAGIALLNDHKLTV
                     YKTRGKVSDLIHSVENKDISGTVGIAHTRWATHGEPNNANAHPHCSQSEELAIVHNGI
                     IENYAVLKQGLIEHGYTFSSETDTEVLVQLIEYVKRKNNVDLPTAVQLALNQIIGAYA
                     IAVIEKGHPDVIVAARKGSPLVVGIGDNEYFLASDATPIIEFTHKVVYVGEEEIVVLQ
                     RGKDLKIKTIGNVEKTPEIKELEMTLSQLEKGGYPHFMLKEIFEQPKTLSDSMRGRIN
                     LEENNISLSGFIDHKEKFLDAKRIIITACGTSWHAGLIGMYAIEEFARIPVEVEYSSE
                     FRYRKPVINKDDLVIAISQSGETADTLAAVQLAKSHGAFIFGICNVVGSSIARNTDSG
                     CYTHVGPEIGVASTKAFTAQVTVLIMLALLIGKEKGTIENEQYLKIVGELAKIPEKIS
                     QILKQNDVIAEFSKTFTYAQNFIYLGRGYNYPVALEGALKLKEISYIHAEGLPAAEMK
                     HGPIALISQEMPVVVIAPSAGKYEKVVSNIQEIKARKGRIISIVTEGDVIVKNLSDYI
                     ISIPETVECLVPLLAVIPLQLLAYHIAVVKGCDVDQPRNLAKSVTVE"
     gene            complement(4933..6159)
                     /locus_tag="PLM04_05805"
     CDS             complement(4933..6159)
                     /locus_tag="PLM04_05805"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HON02624.1"
                     /translation="MALANENYLKIPESRFSDEIEKRVNAFKVLHPTTKLIRLGIGDV
                     TRPLPAESVFAIHKAADEMLHTETFKGYGPLQGYDFLVDKILKNYRSSGIVLDKESIF
                     INHGAKPDIGNIGHILGRDNIIAIAEPVYPVYENAAIMSGRGGEMNEEEKWSNIVYLH
                     CSKENNFVPELPDERVDIIYLCNPNNPTGTVINKTELKKWVDYAIENQSIIIYDGAYH
                     AYIKEPDIPHSIYEVKGAKKVALEIRSYSKTAGFTGLRCGYTVFPKELMVYTRTGEEI
                     PLLKLWYRRNLNYSNGVSYVVQRAAEALYSRKGRMEIQELVNYYLENAKKIRKELLEL
                     GWEVYGGINSPYVWFKTPHNEPSWKFFQELLYNYNIIGMPGIIFGKSGDGYMRFSGFC
                     SHEDTEEAIKRIKSNY"
     gene            6664..7209
                     /locus_tag="PLM04_05810"
     CDS             6664..7209
                     /locus_tag="PLM04_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HON02625.1"
                     /translation="MNQPTSAIISEELIDEIIGKYQNKPGFLLSVLEEIQEKNPLKYL
                     PAETLQTVAAKLNLPYSRVYGVVTFYSFFNLRPQGKHSIVVCRGTACHTKGSKQLLNE
                     IGHSFGMNHQDENSEASFTTADNQFTVRTVACFGQCAQSPVVEVDGKIYSYVNSQKLL
                     KILENVKKNGVQKNFSVNTSR"
     gene            7222..10365
                     /locus_tag="PLM04_05815"
     CDS             7222..10365
                     /locus_tag="PLM04_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HON02626.1"
                     /translation="MAMTVAELELLKNKNIERILPSVPMFLVGMSTCGLGNGAESVYQ
                     SLEKEIELRHLNFKLKQTGCFGYCAEEPIVMMYQPQKPLVVYSRVNEKDISAIIDGII
                     KSKIYRKKILCRIDFWDHLTSKIEYGKDYEDVPHWDEISFFKGQKKIVLRDAGFIDPE
                     SILDYIAVGGYQALSKVLTGMQPDEVIHEVTKSKLRGRGGAGFPTGTKWSIMKRSKAD
                     VKYIICNADEGDPGAYMNRNEIESDPHMLIEGMIIGAYAMEANQGVIYIRAEYPLAVK
                     RFQIAMQQAREYGLLGKNILNTGFNFDIQLVEGAGAFVCGEETALIASIEGKAGRPMP
                     RPPYPAERGLYGKPTNINNVETWCNIPVIIEKGSDWFTKTGTERSAGTKVFSLVGKIK
                     NTGLVELPLGSTLEKFIFEIGEGTGNNKRIKAVQTGGPSGGCIPVEYFSTPVDYESLS
                     SLGAIMGSGGMVVMDQDNCMVDVAKYFLEFSANESCGKCTPCREGLSQALAIMNKISK
                     GEGNFQDIETLELLTRTIRDTAICGLGQTAPNPILTTLRYFKNEYEEHIQEKRCHAGT
                     CESLFLALCENSCPLHMNIPGYIELVKEDRIEEAFELTLRDNPIPGTIGRICHFHCQM
                     RCRREMVDEPVAQGEIHRYLADTMYQLGKENEIYNRLIAEKLPSSGKKIAIVGAGPAG
                     ITAAFYLVRLGHEVTVFDAMPEAGGVLRYGIPQYRLPKSVLKKELKLIKKLGVKFVFN
                     TRIGKDIFLQDLKNNFNAVYLAFGAWKDIDLNIPGENAKGVFVGTDLLKELSLDKTPR
                     IGQKIVVIGGGNVAIDAARSLWRLGKDVTVVYRREKIDMPANKDEIAEAEAEQIHFRF
                     LSAPHEILTDNSGRVNALKIEIMTTGPVDTSGRRKPIATGKIEEIPCDTVVLAVGERV
                     DSAPFVAEGLKVTKDGRLIVHPFTFQTSEPNIFAGGDAVMGPATAAEAMGMAKRAAEA
                     IDWELMKEKRFHLLFKKFEYRNVIPTDIKKSPKNVSAKIQVKERIGNFHEVSTGYTGE
                     QARDEVNRCLRCDVKVN"
     gene            10454..12211
                     /locus_tag="PLM04_05820"
     CDS             10454..12211
                     /locus_tag="PLM04_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HON02627.1"
                     /translation="MKITINHQPVDVKDGITIMEACQSVGIDIPSLCFLKDISSNASC
                     GICVVEVKGARSLLRSCITKVAEGMDILTTSPRVLEARKVNVELLLANHPQDCLQCDR
                     NLNCELQRLSFELGIKERHFVRTRKKYLPKDETSLSLVRDPEKCILCGRCVAVCTDMQ
                     SVNAIDFSGRGLNTSISTFFDKGLGATYCTNCGQCSLVCPTGAITEKTSVPQVWKEIL
                     NPEKMVLIQTAPAVRVGIGEAMGMPYGSLVTGQMVAGLKRLGFSKVFDTNFAADLTIM
                     EEANELMQRIRQNGVLPMITSCSPGWIKFAEHFFPSSLAHLSSCKSPQQMFGAIAKTF
                     YAEQTGIDPRKIVVVSVMPCTAKKYEARRPEMDDAFHYWKEKLQLSEEEHFFDVDYAL
                     TTRELARMFKESGIDFQHLLEEDFDQPLGVSTGAGTIFGATGGVMEAALRTAYEVMTG
                     RQLKDVEFLSVRGMKGIKEAEIDLDGRKIKVAVAHTLKNARFLLEQIQAGISPYTFVE
                     IMTCPGGCLGGGGQPIPTNSEIREKRAASIYREDERKQIRKSHENPEIQYLYEHFLEK
                     PLSEKSHHLLHTHYVERGC"
     gene            12317..12793
                     /locus_tag="PLM04_05825"
     CDS             12317..12793
                     /locus_tag="PLM04_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HON02628.1"
                     /translation="MKTIDCTKLNAEGAKKVVDALQQLLADYQIFYTNLRGFHWNLKG
                     KDFFINHAKFEELYENVAEKVDEIAERILMLGGTPAHNFSDYLKTAKLKETGLVTDGD
                     TGLKNVLESYAYFIESERNIIKLAEAIDDEATVAQMSDYIKEQEKMVWMLVSYFEK"
     gene            12985..14058
                     /locus_tag="PLM04_05830"
     CDS             12985..14058
                     /locus_tag="PLM04_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HON02629.1"
                     /translation="MEQNYCIIMAGGIGSRFWPFSRKNRPKQFLDFFGTGRSLLQLTY
                     DRFRKIIPAENILIVSNELYRDLILEQLPEINDNQLLLEPVRRNTAPCIAYAIYKIKS
                     ITENANIIVSPSDHLILKESEFLETIRQGLEFVSKNDNLLTLGIKPSRPETGYGYIQI
                     EFGESNLRKVKTFTEKPNAELAKLFFESGEFFWNSGIFLWNLKTILAAFDFHLSEISQ
                     KFKQGLKYFNSTEEKKYIADIFHTCPNISIDYGILEKASNVFVLCSDFGWSDLGTWGS
                     LYDISPKDEKENVTLKCKTLYYDSHRNIVALPSGKLAVIQQLNDYIVADSDNVLLICK
                     REDEQQIRQFVKDASILYDGEFD"
     gene            14088..14372
                     /locus_tag="PLM04_05835"
     CDS             14088..14372
                     /locus_tag="PLM04_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02630.1"
                     /translation="MTPSFFNIYKPRKYDYRYIYYDPLKEAIKEREKQMAKENGEQPQ
                     QEYKPNLHRGSFREEANRMRGKKSKELRRSNRNVLIILIILLIIAYYLLK"
     gene            14388..16223
                     /gene="mutL"
                     /locus_tag="PLM04_05840"
     CDS             14388..16223
                     /gene="mutL"
                     /locus_tag="PLM04_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HON02631.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPASVLKELIENSIDANANNI
                     IIQIKNAGRTLIQVTDNGKGMSETDARLAFERHATSKISNVSDIYALQSYGFRGEALA
                     SIAAVAQVELQTRRKEDELGTLIEIEGSKVIRQESVLCEEGTTIRVKNLFFNVPARRR
                     FLKSDNVEKIHLLNEFYRIALVYPDIRFSFFDDKEEIFKLLPSPLKLRIENIFGKGTK
                     RKWEQQLLPIETNTPLVKIEGYVGRPEFALKSAYQYFFVNARYMRHPYFHKAVISAYS
                     QMIRPEENPNYFIYLYVAPETIDVNIHPAKTEIKFENEQAIWSILLATVRETLGKYNV
                     IPSINFNQEDAIDLPIIGNNEKIVPPHTVFNPNFNPFENTLVNKRPPLNWETLYRNFE
                     KKEESKPTSFQWIENDITAYASSASSIIAEETLDSSHFLQLKTKYILTAVKSGLLVIH
                     QHKAHVRILYDLYLSQIKHQKSSSQQLLFPEILELSEHEMLYFSSIKNDLSNVGFLFK
                     SVGKNAFEIFGIPGPLDKMNIQAFIHELIEKFKVKQSISEEDLHQQIALSMAETSAIK
                     TGQKLTQEEMTDLVNRLFATEEPSFSPQGEKILSIIPMEEFDSQF"
     gene            16407..>16684
                     /locus_tag="PLM04_05845"
     CDS             16407..>16684
                     /locus_tag="PLM04_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HON02632.1"
                     /translation="MKLIDEKLLNELTRQAKENARLRVNYNLHEHLEDKVQRLLNAME
                     PGTKLPIHRHRHTAETYFLIRGSILVKYYDDKGNLTEETELNVKEGKYG"
BASE COUNT         5186 a         3128 c         3273 g         5097 t
ORIGIN      
        1 aaaaaatttt catacaaatg taaaacaaat attacatgca aaatttgaac agacggaaga
       61 gaaagagtac ttttgcaaca agaaaattgt tcaatccaaa ttttaaaata gaaaacaggt
      121 ttgaataatt gctcaattct ctaatatttt tgtttttcat tctgttatgc aaccacttat
      181 tgaagcagtc gataaagaag ttctgaaaag agaacttact cccgaaaaat ttttacgccc
      241 tactaataaa tcagataatg aaatttatgt aattactgca cacaattctc ctaacgtaat
      301 gttggaaatc ggtcggttgc gcgaaatggc ttttcgttcg tggggtgggg gaaccggcaa
      361 ggaaattgat gtggatgctt atgattattc ggaaaaaccc tattatcaac tgattgtttg
      421 ggacccggaa gaaaatgaaa ttgtgggcgg ttaccgtttt ttatttggaa aggatgttcc
      481 gttaaatgaa aaaggagaac ccgaatttgt aatgtcgcat cttttcaaat acagtaaaaa
      541 atttatcaac gaatatctgc cttatactat cgaattaggt agagcattta ttcagccagc
      601 ctatcaaacc tctcaaaaag gatcaaagag catatttgca ctcgataatt tgtgggatgg
      661 gttgggagct cttattcatt tggctaagga agcacgatat tttataggaa aagttaccat
      721 gtatcatgct tatccggctg ctgcaaggga gttgatttac gaatatatgt ttcgtttttt
      781 ccccgacaaa gaaaaactga ttttaccacg aaatccggta accattagca acaatattaa
      841 aattttgtca aacaaacttt ttaacgagaa agacaaagag gcaaattaca aaattttaca
      901 aaagatggtg agaaaggaaa acgtaaatat tcctccgctt ttcaatgctt atatcggcct
      961 gacaaataca ctacgtatgt ttggttctgc cattgatcca aacttcagtt cggtttacga
     1021 aaccggaatt atgctgacca tgtccgatat tgtggaaact aaacgcaaaa gatatattga
     1081 accttacatt gagtatttga aaaatgtgtt ttcaaacgga acgttttcca aaattaacgc
     1141 ttacgaatct cactgcagaa acatttataa cttttttcag ttcgagtaac tgctaaaata
     1201 tttaattttt atcttttcct tcgtaatagt cgataaaagc cttgttaacc attctgttgc
     1261 cgcccggcgt gggataatcg ccagtaaaat accagtcgcc tttgtgctgt gggcaagctt
     1321 tgtgtaaatt gtcgatggat tgataaacca gctctaccgg acaatctacc tcatcgggag
     1381 taagcatttt ggcaatttga gacgaaattt cttcctgcgt gaaaggagcg taaatttcct
     1441 tcacataatt ggtaactttt tctttcggcc agttttcctg ttgcttgcat ttttcgtaaa
     1501 tatcgtccat aattttctgc ctgccgctgt ctttaagcaa ttgaactgca gctctgaaag
     1561 cacaaaattc gttcatacgg gccatgtcga taccgtaata atcggggaaa cgtacttgcg
     1621 gagaagagga cacaatcacg atttttttgg gttccaatcg attcaatata tttaaaatac
     1681 tttgtttgag cgttgttccc cgaacgatgg aatcgtcaat cactaccaaa ttgtcttttt
     1741 tctcgggtcg gatggagcca taggtaatat cgtaaacatg agccgccaaa tcgtttcttt
     1801 ctttgttttg tgaaataaag gtgcgcaatt tgatgtcttt cagcagcact ttttcgattc
     1861 gtggaacttt gcccgtcaga gtgcgaatgc catccgtcat tccgtaaaaa gccacttcgg
     1921 ccgtattggg aataaacgaa aacacggtat tatcaatatc ctgatcgact gcttttaaaa
     1981 tagcttcact taaaaatcct cccaattttt ttctttcctg ataaatatct ttgtcgcttc
     2041 cacgtgaaaa atagattctt tcaaaagaac aaggttttgg ttcgggatct gcctcacgaa
     2101 tggtttcgaa atgcatcgat ccgtttttct tgataataag tgcttctccg ggttttaatt
     2161 catgtacctg ctctgctttc acattaatgg ctgtttgaat gaccggacgt tcggaagcag
     2221 caacaataat ttcatcatcg gcgtagtaaa atgcggtcct aattcctttg gggtcgcgga
     2281 aaaggaaggc atcgccgtgt cccaacatcc cgcaaataac gtaacctcca tcccaaatgt
     2341 gttccgactt tcggataaaa gaagcaatgt ccagattttc ttcaattttt cgggttatca
     2401 cctgatcatt ggagtagcgt tgtttgataa tttcatattc acgttgtact tctctgtcga
     2461 gtttgtcgcc gagcgattcc agcatcagaa aagtatccga tttgtcgcgt ggatgttgtc
     2521 cctgtgccgt tagcgactga aaaatttcat caacattggt taggttaaaa tttccagcca
     2581 acaccaatgt tcgcgatttc caattgtgtc tgcgcaggaa cggatgaata tatgacaatc
     2641 cgcttttccc gcttgtgctg tagcgcaaat gtcccaaata aagttctccg gcaaacgggc
     2701 aaaacatttt tgctttggca gcatccgaac tgtaatcttc gggcgccagc gagtgatttt
     2761 tatctatatc gacaaaaatt tcctgaatgg cattttttcc ttctattctt ttacgcgaaa
     2821 tatattcttc tccgggcggc atatccaatt ttacggtggc gattcctgca ccttcctgac
     2881 cccgattgtg ctgcttctcc ataagaagat acattttttg caaaccgtat tcccaagtac
     2941 cgtatttttt ctggtaataa tccaatggct tcaatagcct tatcattgcg attccgcaca
     3001 taataaattt cttattttat ttaaaagaga ttataagatt tttgaaactc aatattttat
     3061 tctacagtta ctgatttcgc cagatttcgg ggttggtcca cgtcacaacc ttttaccaca
     3121 gcaatgtgat aggccaaaag ttgcaacgga atcacagcca gcaaaggaac caagcattct
     3181 acggtttcgg gaatggaaat aatgtaatcc gaaagatttt ttacaattac atcgccttcc
     3241 gttacgatag aaataattct accttttcgt gcttttattt cctgaatgtt gctgacaact
     3301 ttttcgtatt ttcctgcact gggtgctatt acaacaaccg gcatttcctg actgatcaag
     3361 gcaattggtc cgtgtttcat ttcggctgcc ggcagtcctt cggcatgaat ataggaaatt
     3421 tccttcaatt ttaaagctcc ttccaaagct accgggtaat tataacctct acccaaataa
     3481 ataaaatttt gcgcataggt gaatgttttg gaaaattcgg caatgacatc attttgcttt
     3541 aaaatttgac tgattttttc gggaattttt gccagttctc caacaatttt caggtattgt
     3601 tcattttcaa ttgttccctt ttccttcccg ataagcaatg ccagcataat taaaactgta
     3661 acctgagctg taaaagcttt ggtggacgcc acaccaattt cggggccaac gtgtgtataa
     3721 cagcccgaat cggtattgcg tgcaatggat gagcccacca cgttgcatat tccgaaaata
     3781 aatgctccgt gcgatttggc caattgtacg gctgccagtg tgtcggcagt ttcgcccgac
     3841 tgagatatgg ctatcaccag atcatccttg ttgataaccg gcttgcggta tctgaattcc
     3901 gaagaatatt caacttcaac cggaattctg gcaaattctt caatggcata cattcctatt
     3961 aagccggcat gccatgatgt tccacaagcg gttataataa tccgtttggc atccaggaat
     4021 ttttccttgt ggtctataaa tcctgaaaga ctaatgttgt tttcttccaa attgattcgc
     4081 cctctcatgc tgtcgctcaa ggttttgggc tgctcgaaaa tttctttcag cataaaatgt
     4141 ggataaccgc ctttttcgag ctgactgaga gtcatttcga gttctttgat ttcgggagtt
     4201 ttttctacat tcccaatggt tttaattttt aagtcttttc cgcgttgcaa aacgacaatt
     4261 tcctcttctc ccacatacac caccttatgc gtaaattcaa tgatgggagt ggcatccgaa
     4321 gccagaaaat attcgttatc gccaattcct acaacgagcg gactgccttt tcgagctgcc
     4381 acaattacat cgggatgacc tttttcgata acggcaatag cataagctcc tataatttga
     4441 ttcaatgcca gttgcactgc tgttggcaag tccacgttat ttttccgttt tacgtattct
     4501 atgagttgta ccagcacttc ggtatcggtt tcgctcgaaa aagtatatcc atgctcgata
     4561 agtccctgtt tcaaaacggc atagttttca attattccgt tgtgtacaat ggccagttcc
     4621 tcagactgag aacaatgtgg atgtgcattt gcattgttgg gttcaccatg tgtcgcccat
     4681 cttgtatgag cgataccgac agttccactg atgtctttgt tttcgacaga atgaatcaag
     4741 tcggaaactt ttcctcttgt tttataaaca gttaatttat ggtcgttaag caatgcaata
     4801 ccggcactgt catatccacg atattcgagg cgctgcaaac cttttattaa aatgggataa
     4861 gcctcgcgat aacccatgta accaacaata ccacacataa agttaatgat ttaagatttt
     4921 tcttagataa ttttaataat ttgatttgat tcgtttaatg gcctcctctg tatcttcatg
     4981 actgcaaaag ccggaaaaac gcatataacc atcacccgat tttccaaaaa taataccggg
     5041 cattccaata atgttgtaat tgtataaaag ttcctgaaaa aatttccacg aaggttcgtt
     5101 atgaggcgtt ttaaaccaaa catacggaga attaatgcct ccataaactt cccagccaag
     5161 ttctaaaagt tctttacgta tcttttttgc attttccaaa tagtaattga ccagttcttg
     5221 aatttccatt cttcccttac gactgtacaa ggcttcggca gcacgctgaa ctacataaga
     5281 tacaccattg ctgtaattca gatttctgcg ataccaaagt ttcaacaacg gaatttcctc
     5341 gcccgtccgg gtataaacca tcaattcttt cggaaaaacc gtgtaaccac atcgaagccc
     5401 tgtaaaaccg gctgtttttg aataacttct gatttccaaa gcaacttttt tggcaccctt
     5461 gacttcgtaa atgctgtgag gaatatcggg ttctttgata taggcatgat aagctccatc
     5521 atatataata atactttggt tttcaatagc ataatctacc cattttttca gttcggtttt
     5581 gttgattacg gttccggtgg gattattggg gttacaaaga taaatgatgt ctacgcgttc
     5641 gtcagggagt tcgggaacaa aattattttc tttcgaacaa tgcaggtaaa caatattgct
     5701 ccatttttct tcctcattca tttcaccgcc tcttccactc ataatggcag cattttcgta
     5761 aaccggataa accggctcag caatggcaat aatgttatct cgtcccaaaa tgtggccgat
     5821 atttccaata tcgggttttg cgccatgatt aataaaaata ctttccttat cgaggacaat
     5881 gcctgaagaa cgataatttt tgagaatttt atcgaccaaa aaatcatacc cttgcagtgg
     5941 cccgtaacct ttgaaagttt ccgtatgcaa catttcgtct gcagctttgt gaatggcaaa
     6001 aacagattct gcgggtaaag ggcgtgtaac atctccaatc cccaaacgaa tcaatttggt
     6061 ggttggatgc aaaactttga aggcatttac ccgcttttct atttcgtccg aaaaacggga
     6121 ctcgggaatt ttcaagtaat tttcatttgc taatgccatt caggaaatga ttaagcagga
     6181 attttttcct ataaataaag cacaaaatta ctgttttttc attgattatt tttctttcaa
     6241 ttcaaaattt caactataca tttcagtatt tttgtgtttt taatctcctt ttccaataaa
     6301 aacaaactta ttcatctaat tttatttcat tatccaattt ttttctttca aaatgtaact
     6361 tttattttta ataaatctaa gttcttccac atgttttaaa aatgaaagaa aaatattgat
     6421 ttttactttt attaaaaaaa attattttat aatcataaca aaataaaaat gtgtcaatta
     6481 aagaaatttt tccataaata aatctaaaaa attgacaata atcatttaat tttatatttt
     6541 tgtttataga aatattcaaa gtatactgaa acatcggatg attgaaatta tatatttttc
     6601 ttcagatttt caatgactta tttataaatt attagtcaaa taacttttaa aaatgaaggt
     6661 tttatgaacc agccaacttc tgcaattatt tccgaagaac ttattgatga aattattggc
     6721 aaatatcaaa ataaacccgg atttttgctt tcggtgctgg aagagattca ggaaaaaaat
     6781 ccattgaaat atttgcctgc agaaacgctt cagactgttg ccgcaaaact caatttgccc
     6841 tattcgcgag tttatggcgt tgtaactttt tattcttttt tcaatcttcg tccgcaggga
     6901 aaacattcca ttgtggtgtg caggggaaca gcctgtcata ccaaaggttc gaagcagctg
     6961 cttaacgaaa tcggacattc tttcggaatg aatcatcagg atgaaaattc tgaagcttct
     7021 tttacaactg ccgacaatca gtttacagtg cggacggtag cctgttttgg acagtgtgcc
     7081 caatcgcctg tggtagaagt ggatgggaaa atctacagtt atgtaaattc tcaaaagctg
     7141 ctgaaaattc tcgaaaatgt taaaaagaac ggtgttcaaa aaaatttctc tgtaaataca
     7201 tctcgttaac cttaaaatgt tatggctatg acagtggcag aacttgaatt gttaaaaaat
     7261 aaaaatattg aacgaatatt gccttctgtt cccatgtttc tggttggaat gagcacttgc
     7321 ggcttgggaa atggagcaga atcggtatat cagagtttgg aaaaggaaat tgaacttcgc
     7381 catctcaact tcaaactcaa acaaaccggt tgttttggtt attgtgcaga agaaccaatt
     7441 gttatgatgt atcaacctca aaaaccattg gtagtttaca gccgcgtaaa cgaaaaggac
     7501 atttctgcca tcattgatgg gattattaaa tcaaaaattt atcgaaaaaa aattctttgt
     7561 cgtatcgatt tttgggatca tttgacttct aaaatcgagt atggaaaaga ttacgaagac
     7621 gttccacatt gggacgaaat ttcctttttt aaaggacaga aaaaaattgt gttgcgtgat
     7681 gcaggtttca tcgatccaga atctatactt gattatattg ctgtgggtgg ctatcaagct
     7741 ctttcaaaag tattgaccgg aatgcaaccg gacgaagtga ttcatgaagt tacaaaatcc
     7801 aaactcaggg gtagaggagg agctggattt cctaccggta ccaaatggtc aattatgaag
     7861 cgttcaaaag ctgatgtaaa atatattatt tgcaatgcgg atgaaggtga tccgggagct
     7921 tatatgaaca gaaacgaaat tgaaagtgat ccgcacatgc tgattgaagg aatgattatc
     7981 ggagcttatg caatggaagc taatcaggga gttatttata tcagagccga atatccattg
     8041 gctgtaaaaa gattccaaat tgccatgcag caagccagag aatacggatt gctgggcaaa
     8101 aatatattaa ataccggctt taatttcgac attcaactgg ttgaaggagc cggagcattt
     8161 gtttgcggag aggaaacggc attaattgct tctatcgaag ggaaagcagg ccgtcccatg
     8221 cctcgacctc cttatcctgc cgaaagagga ttgtacggca aaccgaccaa tatcaacaac
     8281 gttgaaacat ggtgcaatat cccggtgatt attgaaaagg gaagcgattg gtttaccaaa
     8341 acgggaacgg aaaggagcgc cggaacaaaa gtattttcgt tggttggaaa aattaaaaat
     8401 acaggtttgg tcgaacttcc gttaggttca actttggaaa aatttatttt tgaaataggc
     8461 gaaggtacgg gaaacaacaa acgtatcaaa gccgttcaaa ccggtggccc atcaggaggc
     8521 tgtattccgg tggaatattt cagtacgccg gttgattatg aatctttatc aagtctgggt
     8581 gctatcatgg gctcgggcgg aatggtagta atggatcagg ataattgcat ggtggatgtg
     8641 gcaaaatatt ttttggaatt cagtgccaac gaatcatgcg gcaaatgtac gccttgccgg
     8701 gaaggactgt cgcaagcgct ggccattatg aacaaaatat ccaaaggaga agggaatttt
     8761 caggacatcg aaactttgga attattgacc cgaaccatca gggatactgc catttgcggc
     8821 ttgggacaaa ccgcgcccaa tcccattctt actactttgc gttattttaa aaacgagtac
     8881 gaagaacata ttcaagaaaa acgttgccat gccggtacct gtgaatcgtt gtttctggca
     8941 ctttgtgaaa atagctgtcc gttacacatg aacattcccg gctatatcga attggttaag
     9001 gaagaccgta ttgaagaagc tttcgaactc actttgcgcg ataatcccat acccggtacc
     9061 attggcagaa tttgccattt tcactgtcag atgcgttgtc gacgcgaaat ggttgatgaa
     9121 ccggttgctc agggcgaaat tcatcgatat ttggccgata ccatgtatca gttggggaag
     9181 gaaaatgaaa tttacaaccg gctgattgcc gaaaaacttc cttcctcggg taaaaaaatt
     9241 gccattgttg gtgccggtcc ggctggaatt actgcggcat tttatttggt aagactcgga
     9301 catgaagtta ccgttttcga tgccatgccc gaagccggag gtgttttacg ttacggaata
     9361 cctcaatatc gccttcctaa atcggtactg aaaaaagaac tgaaactgat aaagaaatta
     9421 ggcgttaaat ttgtatttaa tacgcgtatc gggaaagaca tttttttgca agatttgaaa
     9481 aacaatttca atgcggttta tcttgctttt ggagcatgga aagacattga tttgaatatt
     9541 ccgggagaga atgccaaagg agtttttgtc ggaacggatt tattgaagga actttcgctg
     9601 gacaaaacgc ccagaattgg acagaaaatt gttgttattg gcggaggcaa tgtggccatt
     9661 gatgcagcaa gaagtttatg gaggttggga aaagatgtaa cagtggttta tcgtagagaa
     9721 aaaattgata tgcccgccaa taaagacgaa attgccgaag ctgaagcaga gcagattcac
     9781 ttcagatttt tgtctgcccc acatgaaatt ctgaccgata attcaggaag agtcaatgct
     9841 ttgaaaattg aaattatgac tacagggccg gtcgatactt cgggcagaag gaaaccgatt
     9901 gcaaccggta aaattgaaga aattccttgc gatacggttg tgctggctgt gggagaacgt
     9961 gttgattccg ctccttttgt agcagaaggt ctgaaagtta cgaaagacgg aagattaatt
    10021 gttcatcctt ttactttcca aacttctgag ccaaatattt ttgccggagg agacgccgta
    10081 atgggaccgg caacggctgc agaagctatg ggaatggcca agagagcagc agaagccatc
    10141 gattgggagt taatgaagga aaagcgtttc catttgttat tcaaaaaatt tgaatatcgg
    10201 aatgtgattc ccacagacat taaaaaatcg ccgaaaaatg tttctgcaaa aatacaggtt
    10261 aaggaacgaa taggtaattt tcatgaggtt tcgacaggtt acaccggcga acaagctcga
    10321 gatgaagtta accgctgttt gcgttgtgac gtgaaagtca attgattatc tagaaaatta
    10381 tttttcattt ctattttttc catacttcaa cgaaacttaa aaaataacct tatttgaaaa
    10441 ttataatcaa tctatgaaaa tcacaattaa tcaccaaccg gttgatgtaa aagatggaat
    10501 cactataatg gaagcttgcc aatctgtcgg cattgacatt ccttcgcttt gttttctcaa
    10561 agatatttcc agcaatgctt cttgtggcat ttgtgtggta gaagtaaagg gagcgcgcag
    10621 tttattgcgt tcgtgcatta caaaagttgc tgaaggaatg gatattctta ccacatcacc
    10681 acgtgtattg gaagcacgaa aagtcaacgt tgagctgctt ttggccaatc atccgcaaga
    10741 ttgcctgcaa tgcgacagaa atttaaattg tgaattgcaa cgtttatcct ttgaattggg
    10801 aattaaagaa agacattttg tacgtaccag aaaaaaatat cttcccaaag atgaaacttc
    10861 tctttcgctg gttcgcgatc ccgaaaagtg tattttgtgc ggtcgttgcg ttgccgtttg
    10921 taccgacatg cagtctgtga atgccattga tttttcgggt agaggattaa atacgagcat
    10981 ttcaactttt ttcgacaaag gattgggtgc aacctattgt accaactgtg gccaatgttc
    11041 gctggtttgt cccacaggag ccattaccga aaaaacatct gttccacaag tttggaaaga
    11101 aatactcaat ccggagaaaa tggtacttat tcagacagct ccggctgtga gagtggggat
    11161 tggcgaagca atgggcatgc cttatggaag tttggtaacc ggacaaatgg ttgccggtct
    11221 gaaaagattg ggtttctcaa aagttttcga taccaatttt gcagccgatc ttaccataat
    11281 ggaagaagca aacgaattaa tgcaacgtat tcggcaaaat ggagttttac ccatgataac
    11341 gtcctgttcg cccggatgga taaaatttgc cgaacatttt ttcccttcat ctctcgctca
    11401 cttgtcttcc tgcaaatcgc cccagcaaat gtttggtgcc atagccaaaa ctttttatgc
    11461 tgaacagacc ggtatcgatc cgcgaaaaat tgtggttgtt tcggtaatgc cgtgcacagc
    11521 aaaaaaatat gaagcccgcc gacctgaaat ggatgatgcg ttccactatt ggaaagagaa
    11581 attacaactt tcggaagaag agcacttttt tgatgtcgat tatgcactga ctacgcgaga
    11641 attggcaaga atgttcaaag aaagcggaat agatttccaa catttactag aggaagattt
    11701 cgatcagccg ttgggtgttt ctaccggagc cggaacaatt ttcggagcaa ccggcggcgt
    11761 catggaagcg gctttaagaa ccgcttatga agttatgaca ggtcggcaac tgaaggatgt
    11821 tgaatttttg tcagtcagag gtatgaaagg cattaaagag gcggaaattg atctagacgg
    11881 aagaaaaatt aaagttgccg tagctcacac attgaaaaat gcacgttttt tgctggaaca
    11941 gattcaagca gggatttctc cctatacatt cgtggagatt atgacttgtc cgggaggatg
    12001 tttgggaggt ggaggtcagc ctattcctac aaattccgaa ataagggaaa agcgggcagc
    12061 ttccatttat cgtgaagatg aacgcaaaca aatacgaaaa tctcacgaaa atcccgagat
    12121 acagtatctt tacgaacatt ttttggaaaa accgttgagt gagaagtcac atcatttatt
    12181 gcatactcat tacgtagaaa ggggctgttg aattatagat attttctatt atttcattta
    12241 tgttttcagt ttgatttatt gttttaaaca tataattttg ccttaaataa ttaatcaaaa
    12301 aaaggatgta tttattatga aaacaattga ttgtacaaag ctgaatgctg aaggagctaa
    12361 aaaagtggtt gatgccttac aacaactttt ggccgattat cagatttttt acaccaattt
    12421 gcgtggattt cactggaact tgaaaggtaa agattttttc atcaaccatg ccaaatttga
    12481 agaattgtat gaaaatgtag ccgaaaaagt ggatgaaatt gcagaacgca ttttaatgtt
    12541 gggtggaacg ccggcacata atttcagtga ttatcttaaa acagccaagt tgaaagaaac
    12601 cggtttggta actgatggcg acacaggatt gaaaaacgta ctcgaatctt acgcttattt
    12661 tattgaaagc gaaagaaata tcattaaatt ggctgaggct atagacgatg aggctacggt
    12721 ggctcaaatg agtgattata ttaaggaaca ggaaaaaatg gtttggatgc tggtatccta
    12781 ttttgaaaaa taaagaatga accatgtcaa aattaaaaaa cggggttgat attcaatcaa
    12841 tcccgttttt tcgttcagcc tgattttttc tagcatgtta ccaaatttct acaattattt
    12901 tggcaaaaaa agaaaaacac tgtatcttag tggtgttatt ttgttataag cattttttaa
    12961 aacaaattta aagtccaagt atttatggag caaaattatt gcattataat ggcaggcggc
    13021 attgggagcc gtttctggcc ttttagcagg aaaaacaggc caaagcagtt tcttgatttt
    13081 tttggtacgg gaaggtcttt gcttcaattg acttatgacc gttttagaaa aataatcccc
    13141 gcagaaaata ttttgattgt ctccaacgaa ctttatcgcg atttgatatt ggaacagctt
    13201 cccgaaatca acgacaatca attattgctc gaaccggtca gaagaaatac ggcaccatgt
    13261 attgcttacg ccatttataa aataaagtcg attaccgaaa acgcaaatat cattgtttcg
    13321 ccttccgatc atttgatctt aaaagaatca gaatttttgg agactatacg tcaggggctg
    13381 gaatttgttt ccaagaacga caatctactg acattgggca taaagccgag tcgtcccgag
    13441 acaggatacg gttacattca aatagagttt ggagaatcaa atttgagaaa agtaaaaact
    13501 tttaccgaga aaccaaatgc agaattagcc aagttgtttt ttgaatcggg tgagtttttt
    13561 tggaattcgg gaattttttt atggaatttg aaaaccattt tggcagcctt cgattttcat
    13621 ctttccgaaa tatcgcaaaa attcaagcaa gggttaaaat atttcaattc tactgaagaa
    13681 aaaaaatata ttgcagatat ttttcatacc tgtcccaata tttccatcga ttatggtatc
    13741 ttggaaaaag cttccaatgt gtttgttctt tgttccgatt ttggatggtc tgatttggga
    13801 acttggggct cgctgtacga catatcccca aaggacgaaa aagagaatgt tacccttaaa
    13861 tgtaaaactc tttactacga cagccatcgt aatatcgtgg cgttgccttc agggaagctg
    13921 gcagttatac aacaacttaa tgattatatt gttgccgatt cggacaatgt attacttatt
    13981 tgcaagcgag aagatgaaca acaaattcgt cagtttgtta aagatgccag cattctttac
    14041 gatggagaat tcgattgaat ttatactttt taaaaccata aaacattatg acaccaagct
    14101 tttttaacat ttacaagcct cgtaagtacg actatcgcta catttattac gatcctttga
    14161 aagaggcaat taaggaacgt gagaaacaaa tggccaaaga gaatggagaa caacctcagc
    14221 aagaatataa acccaatctg catcgcggtt catttcgaga agaagccaat aggatgagag
    14281 gaaaaaaatc aaaagaatta cgtcgttcga acagaaatgt tcttattatt ctgattattt
    14341 tgctgatcat tgcctattat ctgttgaaat aaaatccaaa tgcctgcatg agtgatatca
    14401 tacatttatt acctgactcg gttgccaacc agattgcagc cggtgaagta attcaaaggc
    14461 cagcttctgt gctgaaagaa ttaattgaaa attccataga tgcaaatgcc aacaacatta
    14521 ttatccaaat aaaaaatgca gggcgcaccc tgattcaggt tacagacaac ggaaaaggaa
    14581 tgagtgaaac cgatgcgagg ctggctttcg aacgtcatgc tacctcaaaa atatcaaatg
    14641 tttcagatat ttatgccttg caaagttatg ggttcagagg cgaagcgctg gcttctattg
    14701 ctgcggtggc tcaagtggaa cttcaaactc gtcggaaaga agacgaattg ggaacgttga
    14761 ttgaaattga aggttcaaaa gtaattcgac aggaatcggt tttatgcgaa gaaggaacaa
    14821 ctatcagagt gaaaaattta tttttcaatg tacctgcccg ccgtcgtttc ctcaaaagcg
    14881 ataatgtaga aaagattcat ttgctcaacg aattttatcg aattgcttta gtttatcccg
    14941 atatccggtt ttcttttttc gacgataaag aagaaatttt caaactctta ccttctccac
    15001 tcaaacttcg tattgaaaat atttttggaa aaggcaccaa aagaaaatgg gaacaacagc
    15061 tgctacccat tgaaaccaat accccgttgg ttaaaattga gggatatgta ggtcgccccg
    15121 aatttgcctt aaaatcggct tatcaatatt tttttgtcaa tgctcgctat atgcggcatc
    15181 cttactttca caaagcagtg atcagtgcat acagtcaaat gattcgtccg gaggaaaatc
    15241 ccaattattt tatctattta tatgttgctc cagaaaccat cgatgtcaat attcatcctg
    15301 ccaaaactga aataaaattt gaaaatgaac aggctatctg gtctattctt ttggctactg
    15361 tccgcgaaac gcttggtaaa tataatgtta ttccatctat caactttaat caggaggatg
    15421 ctatcgacct tcccattatt ggcaataacg aaaaaatcgt accaccccac actgttttca
    15481 atccaaattt caatcccttt gaaaacacat tggttaataa acgaccacca ctcaactggg
    15541 aaacgcttta cagaaatttt gaaaaaaaag aagagagtaa accaacttcc tttcaatgga
    15601 tagaaaacga cattacggct tatgcttcat ccgcttcaag tataattgca gaagagaccc
    15661 tcgacagcag ccatttttta caactcaaaa ccaaatatat tcttactgct gtaaaatcag
    15721 gtttgttggt tattcatcaa cacaaggcgc atgttcgtat tctgtacgat ctttacctat
    15781 cacagatcaa acatcaaaaa agctcttctc aacaattgct tttccctgaa attttagaac
    15841 tttctgaaca tgaaatgctt tatttttcat caataaaaaa tgatttgagc aatgtcggtt
    15901 ttctattcaa atccgtaggt aaaaacgctt tcgaaatttt cggcatacca ggcccgctag
    15961 ataaaatgaa tattcaagca tttatacatg aattgataga aaaattcaaa gtcaaacaat
    16021 caatttccga ggaagatctt catcaacaaa ttgctttgtc tatggcagaa acatcagcaa
    16081 tcaaaaccgg tcaaaaactt acacaagaag aaatgactga cctcgtaaat cgtttgtttg
    16141 ccaccgaaga accatctttt tccccacaag gtgaaaaaat cttatccatt attccaatgg
    16201 aagaatttga tagccaattt taaaactttc atttttccgt ttgtttgctg gcaattaaag
    16261 aaaagttagt atataagttt tcatctgaca attttttata ataggtggtt ccttaaattc
    16321 aatagttcgt taaaaattaa attatctgtc aaataaatcg agtagaaata aaattacgtg
    16381 gttgtctgaa tcaaaattta ttacgcatga aactgattga cgaaaaacta ttaaatgaac
    16441 ttacccgaca ggccaaagaa aatgcccgct tgcgagtcaa ttacaatcta catgaacatt
    16501 tggaagataa ggttcaacgt ttgctcaatg ctatggagcc gggtaccaag ttacctatcc
    16561 acagacatcg acatacggcg gaaacatatt ttttgatacg agggagtatc ttagtgaaat
    16621 actatgatga caagggaaat ctgacggaag aaacagaact gaacgtaaag gaaggaaaat
    16681 atgg
//
LOCUS       DAOTAX010000045        16284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_2968_length_16284_cov_30.745579, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000045 DAOTAX010000000
VERSION     DAOTAX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_16284_cov_30.745579"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLM04_05850"
     CDS             <1..472
                     /locus_tag="PLM04_05850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HON02633.1"
                     /translation="FYDFEAKKINGENISMQQFKGKTVLVVNTASKCGLTPQFEGLEK
                     MYEKYKDKGLVILGFPCNQFAHQDPGSSSEISEFCVVNYGVTFPMFEKVDVNGKNAHP
                     IFKYLKSQLKGGLFGSKIKWNFTKFVIDKNGNPVKRFAPTVTPEKIEKYIEKIL"
     gene            complement(652..1872)
                     /locus_tag="PLM04_05855"
     CDS             complement(652..1872)
                     /locus_tag="PLM04_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02634.1"
                     /translation="MTQSKEKLWTASFISICVANFMLFFAFYLLLPILPLYLMENFQA
                     SKSLVGIILSSYTIAALLIRPIAGFLLDMFQRRPIYLLAYFLFSLCFIGYPLANFLNF
                     FLSLRIVHGLCFGMVSTASNSFVIDIMPSSRRGEGLGYFGIANNLAMSIGPMISLFLH
                     DYSSYQMIFFTSIASSLIGFVVVSTVKAKKTTIPTIKKDKEAIVLDRFFLIKGTHAGI
                     CLTLMGIPYGMLTTYIAIYGKEIGMYSGMGIFFTLMAIGLIFSRFFAGRMVDRGHLLK
                     VITVGTIICTVSIFVLPALKDLFLIQQNFSMILFYVVAVFLGLGYGMLFPAYNTLFVN
                     LAPHNRRATASSTFLTTWDVGIGLGLIIGGKLGDTHNGLSLSFLTGGLIAFSSLVYFI
                     KITAPHYKKNTIIT"
     gene            complement(1885..2433)
                     /locus_tag="PLM04_05860"
     CDS             complement(1885..2433)
                     /locus_tag="PLM04_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HON02635.1"
                     /translation="MIINERNTRRERLMQAANAMMTAARTAPKGKGYDIIEIAIITDE
                     TIVELSNAMFEYSQQSGLKFITRDAENILKSEVILLIGTRQQNQNLNCGYCGFDTCAE
                     KNSQPDIPCALNTVDVGIAIGSACAVAADLKVDTRVMFSVGRVAKEIGLLPGCSSIFG
                     IPLSCSSKNPFFDRVSKTPAPK"
     gene            complement(2495..3292)
                     /locus_tag="PLM04_05865"
     CDS             complement(2495..3292)
                     /locus_tag="PLM04_05865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HON02636.1"
                     /translation="MEIELNQLCLSVCEIARSAGKMIADERKRFDDSKVEYKGLHDLV
                     SYVDKNSEKLIVSQLEKLLPEAGFLAEEGTTSKEGERYNWIIDPLDGTTNFVQGVPVF
                     AVSIGLHEANEMVLGVVYEIGRDECFYAWKGGGAFLNGRAIHVSERDNIQDALLATGF
                     PYSEFSRLNEYMEFLKWAVTNSRGVRRLGSAATDLAYVACGRFEAFWEYNLKAWDVAA
                     GAIIVKEAGGTVTDFSGKNNFLFGKEIVATNQKLKQLFLDKLREMMF"
     gene            3624..5582
                     /gene="gyrB"
                     /locus_tag="PLM04_05870"
     CDS             3624..5582
                     /gene="gyrB"
                     /locus_tag="PLM04_05870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HON02637.1"
                     /translation="MITEEKLNAIAEYDASSIQVLEGLEAVRKRPAMYIGDIGIKGLH
                     HLVYEVVDNSIDEAMAGHCDDIQVTIHPDNSITVTDNGRGIPVDFHEKEGKSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSFMRVEVARNGKIYEQEYERGIPKFP
                     VREIGTTSTTGTRTTFIPDDTIFTEVVYKYDIIADRLRELAFLNAGIRLTLVDERTVE
                     EDGSFRKEVFYSKEGLKEFVRYIDEAREKLIDDIIHIRNEKNGIPVEIAMTYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEESKMLDKVKIEISGDDFREGLTAV
                     ISIKVAEPQFEGQTKTKLGNNEVMGAVDMAVGEALSTYLEENPKAARMIVEKVILAAT
                     ARNAARKAREMVQRKSPLSGGGLPGKLADCVERDPAKCELFLVEGDSAGGTAKQGRSR
                     QFQAILPLRGKILNVEKAMQHKVLESEEIRNIYTALGVTIGTEEDSKALNISKLRYHK
                     IIIMTDADVDGSHIATLIMTFFFRHMKELIEQGYLYIATPPLYLCKKGKIEEYCWTEQ
                     QRQAFIEKYGNGQEGSIHVQRYKGLGEMNETQLWETTMNPEKRTLRQVTIENAASADH
                     VFSMLMGDDVLPRRQFIEQNATYANIDA"
     gene            5679..6230
                     /locus_tag="PLM04_05875"
     CDS             5679..6230
                     /locus_tag="PLM04_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HON02638.1"
                     /translation="MNVAVFCSSSNQIQPVYKQAAFLLGQFIAESGFTLVYGGSTGGT
                     MDDIALGATEKKGKIIGVIAQPIIRMHRQSTLPTELIVVEDLSERKNKMKQLADVFVA
                     LPGSYGTFDEMMDVIASGVVGEHHKPIVVVNEQHFFDDFIRQINRMKIEQFLPEMENY
                     RPVFVDNINQCKGFLQSLSIRNY"
     gene            6245..7567
                     /locus_tag="PLM04_05880"
     CDS             6245..7567
                     /locus_tag="PLM04_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02639.1"
                     /translation="MALFGNKFFRKITPTGKFEKSENERIQSLQRYNEIARSAELAEY
                     QQLSKVISSPKFQENKKILKNRKYKDTEEYRLSSEYKKLQNSHSLQQYFKTLESKELN
                     DFLKFKETPDYLDLGNRNKVKASEELKKFKQFEHSKEYKNFVRYHDSFVVKEYEELKE
                     KVNSPEFKKSNDFWANPDRWHTTKEYALQRRHHQLQKNPDIIYYENENPERFGIFRSL
                     ELTFEENFDWNTLDSSRWKYGFHYKSPQLIGDHSFANEKQANNSGKNVIVSDGRLKIF
                     TKEEKVTARAWDIQKGFIMKEFDYTSDVIQTSDSFRQKYGMFRAKLRCSGKIHHAFWL
                     GGDEMLPHINIFHFDGKYINVGNASKNEMDGTKIKGIHPSEFYIYSLVWTEKELIWMI
                     NNYEVYRTVRNIPDEELYLALNSFIPEKEKGDKGVLEVDWIKVYQFKQ"
     gene            7604..8416
                     /gene="kdsA"
                     /locus_tag="PLM04_05885"
                     /pseudo
     CDS             7604..8416
                     /gene="kdsA"
                     /locus_tag="PLM04_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
     gene            8447..8971
                     /locus_tag="PLM04_05890"
     CDS             8447..8971
                     /locus_tag="PLM04_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HON02640.1"
                     /translation="MVNQPVEKVQLPQKEKQKIKLLLSDIDGVMTDGGLYYSEFGDQS
                     KKFHVRDGMGLKILQSKGIKVGIVTSEDNKIAEMRYNKLQLDYLYKGRKNGGKLAAAL
                     EICEKEGISLQQTAYIGDDVNCYELLCSVGLAACPADAMELIKSVPGIIQMKAKGGQG
                     CLREFVEYILKNYC"
     gene            8984..9940
                     /locus_tag="PLM04_05895"
     CDS             8984..9940
                     /locus_tag="PLM04_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HON02641.1"
                     /translation="MDFVKRSKEIFEQEISELQKLAEKIGPEINRAVELIYNCKGKLV
                     ILGIGKTGIIGHKIASSLASTGTQTIFINAAEAMHGDLGMIDRNDIALLISNSGSSPE
                     IVNLITPLKKIGCTIIAMTGNPKSPLAREASLIINTGIEREACPLGLAPTTSTTATLV
                     MGDALTICLMERRNFKAENYALYHPGGALGRQLLARVKDQMNHDIPKVYTHTLFKDVI
                     YEVSNKRLGMTMVYDDNENAVGIITDGDIRRAIQRFDDLKNLTAQEFMTHGFKKIAQN
                     EMLSEALEIMDENNITNLAVMENDSSSTVVGILSIHDIIDFK"
     gene            9953..10798
                     /locus_tag="PLM04_05900"
     CDS             9953..10798
                     /locus_tag="PLM04_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON02642.1"
                     /translation="MFKKIYIEITNSCNFHCSFCFTTSQPPRFMPVKDFHTIVRKIKP
                     FTNYIYLHVLGEPFLHPHIEDILNVAEQEGMNVNITTNGSLLNNRKKLLLNHSIRQLN
                     ISLHDAEENIPSIHWESFFENIFNIAMEISAKTYVNLRLWNIDNPVSGKFNSLCKEKI
                     VEKFQLSPDELTQPEKGNSIKLANHIFLQQSPKFQWPTENGEPLFEHKSCYALRDHIA
                     ILAGGEVVPCCLDAGGSLKLGNIFTEELSDILMCERAKKMKQGFRQHKITEPFCKTCG
                     FSVAF"
     gene            complement(10820..11878)
                     /locus_tag="PLM04_05905"
     CDS             complement(10820..11878)
                     /locus_tag="PLM04_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HON02643.1"
                     /translation="MTAELANEAIQLLRQMIAIPSFSKEEKEIADFLEQYLTERGYVV
                     FRHLNNIWLQSPGFDVSRPTILLNSHLDTVKPGSSWIRNPFEASLKNGKLFGLGSNDA
                     GASLVSLLQVFFYLTQRPQPYNLIFAATAEEEISGKNGIEIMLQEFPKVDFAIVGEPT
                     GMNMAVAERGLMVIDATVTGKSGHAAREEGENAIYKALDAINWFKNYRFAKVSPLLGP
                     VKMTVTQINGGIQHNMIPDKCTFVVDVRSNELYSNEEILEIIRENVDCRIEPRSVRLC
                     STATQPDHPIIKRAQQMNLTLYGSPTLSDQALMPFPSVKIGPGDSARSHTADEYVLVE
                     EIEKGIEVYIQLLDGLQI"
     gene            complement(11940..12752)
                     /gene="argB"
                     /locus_tag="PLM04_05910"
     CDS             complement(11940..12752)
                     /gene="argB"
                     /locus_tag="PLM04_05910"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HON02644.1"
                     /translation="MNSDKSPNIDMFQKKEKLTIIKVGGKIVEESDSLISLLNDFSKI
                     DGYKVLVHGGGRSATALATRLGLETKMIEGRRVTDAEMLKVVTMIYAGWVNKQIVAGL
                     QALGLNALGLTGADLNYMRSEKRSPYPIDYGFVGDVKEVNVSLLTDLIRKEVVPVLAP
                     LTHDKCGNLLNTNADTIAAEAAKAFSSFFEVTLIYCFEKKGVLMNEKDEDSVISSLTP
                     ELFDKFVSQKVIQGGMIPKMENSFNALKSGVSKVIITRADAILTHSGTTITL"
     gene            complement(12824..13348)
                     /locus_tag="PLM04_05915"
     CDS             complement(12824..13348)
                     /locus_tag="PLM04_05915"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HON02645.1"
                     /translation="MDVEQVIASIRNIPDFPKKGVLFKDITTAMKDAEVLRFISDELY
                     EYYKDKGITKVVGLESRGFMLGSILAYKLHAGFVLIRKAGKLPAATYRVDYELEYGVD
                     SLEIHKDAIEKDDVVLLHDDLLATGGSANAALTLLDNFKPKAIYVSFLIELKDLKGRD
                     RLKGVQDVHSLIQF"
     gene            complement(13412..14365)
                     /locus_tag="PLM04_05920"
     CDS             complement(13412..14365)
                     /locus_tag="PLM04_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HON02646.1"
                     /translation="MKTFIRVQDLGNLQEALQEAFEVKKNRFGYQHLGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTIVLDINQGAWKLETERGVVMDGDKTEHILEAIPVMGN
                     YCDLIGVRSFAQFENKAYDYQETILQQFIQYSGRPVFSMESATVHPLQAFADLITIEE
                     YRKKPHPKVVMSWAPHPKALPQAVPNSFADFMNEADVEFVITHPKGYELAPEFVRNAR
                     IEYDQMKAFEGADFVYAKNWAAYQDPNYGKIICTDRSWTVSSRQMAVTNNAYFMHCLP
                     VRRNMIVTDEVIESLQSLVIPEAANREISAEVVIKRMLESL"
     gene            15136..16209
                     /locus_tag="PLM04_05925"
     CDS             15136..16209
                     /locus_tag="PLM04_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON02647.1"
                     /translation="MKKNNYFLLAVFTAVVAFTSQQAKAFPPYIYEGFDYEIGATPLN
                     YNPLVPGTWGAVHMWKPASSWNGYGGSFSNTFPWGVTDVSPMVVPSLVQTGKYFNGNG
                     EWVSCFLREINTDWNSPYWDYMDESKLGKSGTTLWFSVILRPQKNKKEAALGLCKAYN
                     EQSVMKIGAFGGNYWGIRVNNDAEQALSTVPIVNDEAKFLVCKVEYTDVTGGTITLYI
                     DPTPGVEPSNAAATLTTTSDIRFKSVQLLTHYSGGCMAADEIRIATTYDEVAPSTGPS
                     TGLVQDMKKDIRVYSRDGKVVADLTMLKGLSTVSVIDTKGAVVKTIQNVGAESVTFDV
                     ANKGLYMVRVQNSGKSATLKVVL"
BASE COUNT         5297 a         3194 c         3162 g         4631 t
ORIGIN      
        1 tttttacgat ttcgaagcaa aaaaaatcaa cggtgagaat atttccatgc agcaatttaa
       61 aggaaaaacc gtgttggtag ttaacactgc aagtaaatgt ggattgacac ctcaatttga
      121 ggggcttgaa aaaatgtatg aaaaatacaa ggataaagga ttggttattt tgggctttcc
      181 atgcaatcag tttgcgcatc aggacccggg ttcatcaagc gaaatcagtg aattttgcgt
      241 tgtaaattat ggcgtaacct tcccgatgtt tgaaaaagta gatgtaaatg gaaaaaacgc
      301 ccacccaatt tttaaatatc tcaaatcgca attgaaaggt ggattgtttg gaagtaaaat
      361 taaatggaac ttcacaaaat ttgtcatcga caaaaacggc aatccagtaa aaagatttgc
      421 acctaccgta actccggaaa aaatagaaaa atatatagaa aaaattttat aatgtgttga
      481 aaaacaaatt tataattaaa taaaatttat tttaaaaaga aagaaggtat tccaaaaaat
      541 actttctttt ttattttaag cagccgcttg aggagtaagt tttgattggg aattgccttt
      601 cattatattc cttggaatat gcgaataata aaaaacagga atttaaatcc atcatgtaat
      661 gatggtattt ttcttataat gaggggctgt aatttttata aaatagacca gcgatgaaaa
      721 agcaatcaat cccccagtta aaaacgaaag cgaaagtccg ttatgcgtat cgccaagttt
      781 tcctccaata atcaaaccca gtcctattcc tacgtcccaa gtagtcagga atgtggaact
      841 tgccgttgcc cggcgattgt gaggcgccaa gttgacaaac aaagtattgt aagcggggaa
      901 tagcattcca taacccaaac cgagaaaaac ggccaccaca taaaacaaaa tcatggaaaa
      961 attttgctga ataagaaaca aatctttcaa tgcaggcaaa acaaaaatgg aaaccgtaca
     1021 gataattgtt ccaacggtaa taactttcaa taaatgtcct ctatccacca tcctgccggc
     1081 aaaaaatctc gaaaaaatca gtccaatggc catcaaagta aaaaaaatgc ccatgcccga
     1141 atacataccg atttctttcc cgtaaatggc tatataagtg gtcaacatac cataaggaat
     1201 tcccattaat gtcagacaaa tgccggcatg agttccctta atgaggaaaa aacggtccaa
     1261 cacaatcgct tccttgtctt tttttatagt aggaattgtg gttttcttgg cttttacggt
     1321 tgaaacaaca acaaagccta tcaatgagct ggcaatggat gtaaagaaaa tcatttgata
     1381 agaagaataa tcgtgtaaaa acaagctaat cattggccca atagacatgg ccaaattgtt
     1441 ggcaatgcca aaataaccca atccttctcc tctcctcgaa gaaggcataa tatcaataac
     1501 aaagctatta cttgctgtgg ataccatacc gaagcacaat ccgtgaacaa tacgcaggga
     1561 caaaaagaaa ttcaagaaat tggccagcgg ataacctata aaacagagcg aaaaaagaaa
     1621 ataagccaac agataaatgg ggcgccgttg aaacatatcc agcaaaaaac cggctatagg
     1681 tcgaatgagc aaagctgcta tcgtataact ggacaaaatg atgccaacaa gcgatttgct
     1741 ggcttggaaa ttttccatca aataaagtgg aagtatgggc agcaagaggt aaaatgcaaa
     1801 aaacagcata aagttggcaa cacaaatgct gataaaactt gccgtccaga gtttctcttt
     1861 ggactgagtc attattttag aaaattattt aggagccggt gttttggaaa cacgatcgaa
     1921 aaatggattt ttggacgaac aactgagcgg aattccgaaa atggaactgc atccgggcaa
     1981 caaacctatt tctttcgcca ctcttccaac ggaaaacata acacgtgtat ccactttcag
     2041 gtcagcagct acagcacaag ccgagccaat agcaatacct acatctaccg tgttcaaagc
     2101 acagggaatg tcaggttgac tattcttttc ggcacacgta tcgaaaccac agtaaccaca
     2161 attcaaattc tgattttgtt gtcgggtgcc aattaataaa attacttccg atttcagaat
     2221 attttcggca tcgcgcgtaa taaatttcaa tccggattgt tgactgtact cgaacatggc
     2281 atttgacaat tccacaatag tttcatcagt aatgatcgct atttcgatta tatcgtatcc
     2341 tttacctttg ggagccgtac gggcagctgt catcatggca ttggccgctt gcattaagcg
     2401 ttcgcgacgt gtattgcgtt cattgataat cataataaga gttttttaca gattccttta
     2461 ttgctataac caaactaaaa gtttagcagt gcatttaaaa catcatttct ctcagcttgt
     2521 caagaaaaag ctgtttcagt ttttgattgg tagctacgat ctccttccca aacaaaaaat
     2581 tgttttttcc tgaaaaatcg gtaactgttc cgcctgcttc cttcacaata attgcacccg
     2641 cagctacatc ccaagcctta agattgtatt cccaaaaagc ttcgaatcgg ccgcaagcta
     2701 cataagccaa atcagtggca gccgaaccga gccggcgaac cccacgtgaa tttgttacgg
     2761 cccactttag aaattccata tattcgttta gccggctgaa ctccgaataa gggaaaccgg
     2821 tagcgagcaa tgcatcctga atattatcgc gttcagaaac atggatggct ctaccgttga
     2881 gaaaagctcc acctcctttc catgcataaa aacattcatc tcttcctatt tcgtaaacca
     2941 ccccaagaac catttcattg gcttcatgca agccaatgct tacggcaaaa acaggaacac
     3001 cctgaacaaa attggtcgta ccatccaacg gatcaataat ccagttgtaa cgctctcctt
     3061 ctttcgatgt tgtaccttct tccgctaaaa aaccggcttc aggcaaaagt ttttcaagtt
     3121 ggctgacaat cagcttttcc gaatttttgt caacataact caccaaatca tgtaaacctt
     3181 tgtattccac tttgctgtca tcaaatcttt tccgttcgtc ggcgatcatc tttcccgccg
     3241 atcgggcaat ttcacatacc gaaaggcaaa gttgattgag ttctatctcc atgtcttttt
     3301 ttaattaaaa atcgactgca aagttagcct ttttttgaga aacatggaca aaaatattga
     3361 ctgctgtcga caacaagcat tccatttttc gtgataaatt tattcaataa tcgagcacaa
     3421 aaagcatgtc gttaaaatgt tcaaaaaact ttattaaatc gggattttaa aattctgaaa
     3481 tagtgatgag atgttgtata tttcgctcaa aaaacgaggt tatctcaaat atttttatta
     3541 aatttgtatg ttttttcaaa acactttttt cggttgaaaa atggcttatt aatagagaaa
     3601 aaaataaaat taactatata gaaatgatta cagaagaaaa gttaaatgct attgccgaat
     3661 atgatgccag cagcattcaa gtcctcgaag gactcgaagc tgtacgcaaa cgtcctgcca
     3721 tgtatattgg ggatataggc atcaaaggat tacatcattt ggtttatgaa gtggtagata
     3781 attctatcga cgaagcgatg gcaggacatt gtgatgatat tcaagttacc atccatccgg
     3841 ataattccat tactgtaact gataacggac gcggtattcc ggtcgatttt cacgaaaaag
     3901 aaggaaaatc ggcattggaa gttgtaatga cggtgcttca cgccggagga aaatttgaca
     3961 agggaagtta caaagtttca ggaggtttgc atggggtggg agtgtcttgt gtaaatgcgc
     4021 tttcgtcgtt tatgcgcgtg gaagttgcca gaaacggtaa aatttacgaa caggaatatg
     4081 aacgcggtat tcccaaattc cctgtaagag aaattggaac tacttctaca acaggcacac
     4141 gcactacttt catccctgat gatacgattt tcacggaagt cgtttacaaa tacgacatca
     4201 tcgccgaccg ccttcgcgag ttggctttct tgaatgccgg tattcgattg acacttgtag
     4261 atgagagaac agtggaagaa gatggaagtt tcaggaaaga agttttctat tccaaagaag
     4321 gactcaagga gtttgtcagg tacatagatg aagctcgcga aaaactgatt gatgacatta
     4381 ttcacatcag aaatgaaaaa aatggcattc cggtagaaat cgccatgact tacaatactt
     4441 cctacaatga aaacattcac tcttatgtga acaacatcaa caccattgag ggaggaacgc
     4501 atttggccgg tttccgaaga ggactgacca gaacgctgaa aaaatatgcc gaagaaagca
     4561 aaatgctcga taaggttaaa attgaaatca gtggcgatga ctttcgtgaa ggactgacgg
     4621 ctgtcatttc gataaaagtt gcagaacctc agtttgaagg acaaacaaaa accaagcttg
     4681 gcaacaacga agtaatggga gcagtggata tggctgttgg agaagctttg agtacctatc
     4741 tcgaagaaaa tccaaaagca gcccgaatga ttgttgaaaa agtgattctc gctgccacag
     4801 ctcgaaatgc agctcgcaaa gcacgcgaaa tggttcagcg caaatcgccc ctttcgggtg
     4861 gaggactacc cggaaaactt gccgactgtg tagaaagaga tcctgccaaa tgtgaattgt
     4921 tccttgtcga aggagattcg gccggcggaa cagcaaaaca gggacgtagc cgtcaattcc
     4981 aagctatttt gccgctgagg ggaaaaattt tgaatgtgga aaaagccatg caacacaaag
     5041 tgctggaaag tgaagaaata cgcaatatat atactgcttt gggcgttact ataggaacag
     5101 aagaagacag caaggctttg aacatcagta aacttcgata tcataaaatc ataatcatga
     5161 ccgatgctga tgtggatggg agccatattg ccacgttgat tatgaccttt ttcttccgac
     5221 atatgaaaga actgatcgaa caaggttatc tatatatcgc tacacctcct ctctaccttt
     5281 gcaaaaaagg aaaaatagaa gagtattgct ggacagaaca gcaacgtcag gcttttatag
     5341 aaaaatacgg aaatggtcag gaaggttcca tacatgttca gcgttacaaa ggtttgggtg
     5401 aaatgaacga aacgcaactt tgggaaacaa ccatgaatcc tgaaaaacgt acacttcggc
     5461 aggtaaccat cgaaaatgcc gcttccgccg atcatgtttt ttcgatgctt atgggagacg
     5521 acgtgttgcc gcgacgccag tttatcgaac aaaatgccac ttacgccaac attgacgcat
     5581 aacatgcttt tcaaaaattt ccttccactt taaaacactt aaaagtggaa ggaaaaattt
     5641 atataaacaa gctgtgagcg cttgaaaaaa gaaattaaat gaacgttgcc gtattttgtt
     5701 cttccagtaa tcaaattcaa cctgtttaca aacaagctgc ttttctgctg ggccaattta
     5761 ttgccgaaag cgggtttact cttgtatatg gaggttccac aggaggaaca atggatgaca
     5821 tagctttggg agctaccgaa aaaaaaggaa aaattattgg cgtcatcgct cagcctatta
     5881 tcagaatgca tcgccagagt acattaccca cagaactcat cgttgtagaa gacttaagcg
     5941 agagaaaaaa caaaatgaaa caattggctg atgttttcgt tgctctaccg ggaagttatg
     6001 gaactttcga cgaaatgatg gatgtaattg cttccggtgt ggttggagaa catcataaac
     6061 cgatagtggt agtaaatgaa caacatttct ttgatgattt tatcaggcaa ataaacagaa
     6121 tgaaaatcga acaatttttg cctgagatgg aaaattatcg acctgttttt gttgataaca
     6181 tcaatcaatg caagggattt ctccaatcac tttcaataag aaactactga aatacaaaaa
     6241 aaatatggct ctatttggga ataagttttt taggaaaatc actcctaccg gtaaatttga
     6301 aaaaagtgaa aacgagcgta ttcaatcgtt gcaacgttac aacgaaatag caagatcggc
     6361 agaacttgca gaataccagc aattatccaa agtcattagt tctccaaaat ttcaggaaaa
     6421 caaaaaaatt ctgaaaaaca ggaagtacaa ggatacggaa gaatatcgac tgagttcaga
     6481 atacaaaaaa cttcaaaatt cgcattcact gcagcaatac ttcaaaacac tggaatcgaa
     6541 agaattaaac gattttttga aattcaagga aactcctgat tatctcgatt tgggcaaccg
     6601 aaacaaagta aaagcttcgg aagaattaaa aaaattcaaa cagtttgaac attctaagga
     6661 atacaaaaat tttgttcgtt atcatgactc tttcgtggta aaagaatacg aagagctgaa
     6721 agaaaaagta aattcacccg aattcaaaaa atcgaacgat ttttgggcca accccgacag
     6781 atggcacacc acaaaagaat atgcccttca gcgtcgtcat caccaactcc aaaaaaatcc
     6841 ggatattatt tattacgaaa acgaaaatcc cgaaagattc ggaatatttc gttcactgga
     6901 actgacattt gaagaaaatt tcgattggaa tacgcttgac agttctcggt ggaagtatgg
     6961 ttttcattat aaaagccctc agcttatcgg cgatcactct tttgccaacg aaaagcaagc
     7021 caacaattca ggtaaaaatg taatagtttc tgatgggcgt ttaaaaattt tcaccaaaga
     7081 agaaaaagtt actgctcgcg cttgggatat tcagaaagga tttataatga aggaattcga
     7141 ttacacttcc gatgttattc agacgtccga ctctttccgc caaaaatacg gaatgttcag
     7201 ggcaaaactt cgttgcagcg ggaagataca tcatgctttc tggctgggtg gtgatgaaat
     7261 gttgccacac atcaatatct ttcatttcga cggaaaatac atcaatgtcg gtaatgcttc
     7321 aaaaaatgaa atggatggaa ccaaaatcaa gggaattcat ccttccgaat tttatattta
     7381 cagtttagtg tggacagaaa aggaactgat ttggatgatt aacaattacg aagtgtaccg
     7441 aacggtaaga aatataccgg atgaagagtt gtatctggca ctcaattcat ttattcccga
     7501 aaaggaaaag ggcgataaag gtgttttgga agtggactgg ataaaagttt atcaatttaa
     7561 acaataatct tccttttttt aaaaaaatca aacttacggt ttcatgattg aaacaaaaaa
     7621 aataaatgtt cttcatcagt ttgatatagg cgatggcggt cgctttgtgc tcatagccgg
     7681 gccttgcgcc attgaatcgg aagccatgac tatggaagtg gccggaacgc tgaaagaaat
     7741 ctgtcgggaa ttgaatatcc atttgatttt taaatcttct tttgacaaag ccaatcggac
     7801 ttcattgaaa tcggctcgtg gcgttggtat ggagcgcgga cttgaaattt tacaaaaagt
     7861 gaagaatgaa tttcaacttc ccgtactgac tgatgttcac gaaacatggc aatgccaacc
     7921 tgttgccgac gttgtggatg tgctgcaaat tcctgctttt ctttctcgac aaaccgacct
     7981 tttgacagct gccgccaaaa ccgggaaaat tgtaaacgta aaaaaaggtc agttcatggc
     8041 cccctgggac atgaaaaatg tggtggataa aatgttggaa gcaggaaacg acaaaattct
     8101 gctttgtgag cgcggttctt cctttggata caataatttg gtggtggata tgaccggatt
     8161 ggtagaaatg cgaaaatatg gtttccctat agttttcgac gccactcatt ccgtacaaaa
     8221 gcccggaggg cagggaaatt caacaggagg aaacagagaa atggttcctt atttgatgcg
     8281 ggcggcactg gctgtcggcg tggatgcagt ttttgccgaa gttcatcctc aacccgatta
     8341 tgcaatttcc gatggcccga atcaacttta tctttccgat gttcgtaaca tcttacagca
     8401 agccatttta attgacaatg ttacgaaaaa cctttccgaa aaagaaatgg tgaatcaacc
     8461 agttgaaaaa gtacagttgc ctcagaaaga aaaacaaaaa atcaagttgt tgctctccga
     8521 tatagatgga gtaatgacag atggaggctt gtattattct gaattcggcg accaatcaaa
     8581 aaaatttcat gtacgtgacg gcatgggttt aaaaatttta cagtcgaaag gaataaaagt
     8641 tggcattgtt acttccgaag acaacaaaat tgcagaaatg cgatacaaca aacttcagct
     8701 cgattacctg tacaagggca gaaaaaacgg aggaaaattg gctgcagcat tagagatttg
     8761 cgaaaaagaa ggcattagtt tgcagcaaac agcctatatt ggcgatgatg tgaattgtta
     8821 cgaattgcta tgctcggttg gtttggctgc atgtcctgcc gatgcaatgg aactgataaa
     8881 aagcgtgccg ggaattattc aaatgaaagc aaaaggtgga caaggctgtc tgcgtgaatt
     8941 tgtggaatat attctaaaaa attactgttg atatattaaa atcatggatt ttgtaaaaag
     9001 gagtaaagaa atttttgaac aggaaatatc ggaacttcaa aaattggccg aaaaaattgg
     9061 acctgaaatt aaccgggctg tagagttgat ttacaactgc aagggaaaac tggtaatttt
     9121 aggaatagga aaaacgggta tcattggaca taaaattgct tcttcactgg cctctaccgg
     9181 tacgcaaacc attttcatca atgcggctga agccatgcac ggcgatttgg gaatgattga
     9241 cagaaacgac atcgctcttt tgatttccaa cagcggaagt tctccggaaa ttgtcaacct
     9301 gattactcca ctgaaaaaaa tcggctgcac cattatcgcc atgaccggga atccgaaatc
     9361 tccactggct cgagaagctt cattgataat caataccggt attgagcgcg aagcctgtcc
     9421 tttgggactg gcacccacaa cttctactac cgcaacgctg gttatgggcg atgcgttgac
     9481 aatatgcttg atggaaagac gaaacttcaa ggcagaaaat tatgctcttt atcatcccgg
     9541 aggagcgctg ggacggcaat tgctggcacg ggttaaagac caaatgaacc acgatattcc
     9601 aaaagtttat acccatactc tttttaagga tgtaatttat gaagtgagca acaaacgact
     9661 gggaatgaca atggtatatg atgacaacga aaatgccgta ggcatcatta ccgatggaga
     9721 tattcgacgt gctattcaac gattcgatga tttaaagaat ctgactgctc aggaatttat
     9781 gacacatgga tttaagaaaa ttgctcagaa tgaaatgctt agtgaggcat tggaaattat
     9841 ggacgaaaac aacattacca atctggcagt tatggaaaat gactcttcct caactgtagt
     9901 cggcattttg tcaattcatg acattatcga tttcaaatga agtgaacaac ccatgttcaa
     9961 aaaaatttac attgaaatca ccaacagctg caattttcac tgcagctttt gttttactac
    10021 atctcaacca ccgagattta tgccggtgaa agattttcac acaatcgtcc ggaaaattaa
    10081 accttttacc aattatattt atctgcatgt gttgggagaa ccttttcttc accctcatat
    10141 tgaagatatt ctgaatgttg ccgaacagga aggaatgaat gtcaatatca ccacaaacgg
    10201 ctctttactc aataatcgga agaaattact tttgaaccat tccattcgtc aactgaacat
    10261 ttcgctgcat gatgccgaag aaaacattcc ttccattcat tgggaaagct ttttcgaaaa
    10321 tattttcaac atcgcaatgg agatttcggc caaaacttat gtcaatttgc gtttgtggaa
    10381 cattgataat cctgtaagcg gaaaattcaa cagcttgtgt aaggaaaaaa tcgtcgaaaa
    10441 atttcaactt tcacccgatg aattaacaca gcctgaaaaa ggaaacagca taaagctcgc
    10501 caatcatatt tttctgcagc aatcgcctaa atttcaatgg ccaacagaaa acggagaacc
    10561 tctctttgaa cacaaatcct gttatgcttt acgtgatcac atcgccattc tggccggagg
    10621 agaagttgtt ccttgttgcc ttgatgcagg cggatcgctg aaactgggaa atatttttac
    10681 ggaagaactt tcggacatcc ttatgtgcga aagagctaaa aaaatgaaac agggctttcg
    10741 gcaacacaaa attactgagc cattttgtaa aacttgcggg tttagcgttg ctttttgaag
    10801 tgcattttca gcaaatttct caaatttgca aaccgtccag caattgaata taaacctcaa
    10861 ttcctttctc gatttcttct acaagaacat actcatccgc cgtatgggaa cgagcggaat
    10921 cgccgggacc aattttaacg cttggaaacg gcattaatgc ctgatcggaa agcgttggag
    10981 aaccgtataa cgtaaggttc atttgctgcg ctctcttaat aatgggatga tccggctgag
    11041 tggcagttga acacagtcga acagaacgag gttcaatacg gcaatcgaca ttttcacgga
    11101 taatttccag aatttcttca ttggaataaa gttcgttgct tcttacgtcg actacaaacg
    11161 tacacttatc gggaatcata ttgtgttgaa tgccgccatt tatttgggta accgtcatct
    11221 tgaccggccc aagaagcgga gaaactttag caaaacgata atttttaaac caattgattg
    11281 catccaacgc cttgtaaata gcattttcac cttcttcacg cgccgcatga cccgattttc
    11341 ccgttaccgt tgcgtctatc accattaatc ctctttcggc aacagccata ttcattcctg
    11401 taggttcacc aacaatggca aaatcaacct ttggaaattc ctgcaacatg atttcaatgc
    11461 cgtttttccc cgaaatttct tcttcggccg ttgcagcaaa aatcaaatta tagggttgag
    11521 gtcgctgcgt cagatagaaa aaaacttgca gcaaacttac caaactggca ccggcatcat
    11581 tacttcccaa tccaaacaat tttccatttt tcagagaagc ttcaaaggga ttgcgtatcc
    11641 aactgcttcc gggttttacg gtatccaagt gggaattgag caggatagtc ggccgagaaa
    11701 catcaaaacc cggactttgc aaccaaatgt tgtttaaatg tcggaaaacg acatatccac
    11761 gttctgtcag atattgttcc agaaaatcgg ctatctcttt ttcttccttg ctgaaagaag
    11821 gaattgctat catttgccgc aaaagttgaa tagcttcatt tgcaagttct gctgtcattt
    11881 tcgttgtaat atgatgagaa ataaaaaaat caattcctca acaagaaaat ttaaagtttt
    11941 tataaagtta tcgttgttcc cgaatgcgtg aggatagcgt cggctcgtgt aataatcacc
    12001 tttgaaactc cggacttcaa agcattgaaa gaattttcca ttttgggaat cattcctccc
    12061 tgaatgactt tttgagacac gaatttatcg aaaagttccg gcgttaaaga agaaattaca
    12121 ctatcttcgt ctttttcatt cattaaaacg ccttttttct cgaaacaata aataagtgta
    12181 acttcaaaaa aagaagaaaa agcttttgca gcttcggcgg caatcgtatc ggcattggta
    12241 ttcaaaagat taccgcattt atcgtgagtg agcggagcca acaccggcac cacttctttc
    12301 ctgatcaaat cggttaaaag gctcacattt acttccttca catcgccaac aaatccataa
    12361 tctataggat agggcgacct tttttcggaa cgcatgtaat tcaaatcggc accggtaaga
    12421 cccaaagcat tcaatcccaa agcctgcaga cctgccacaa tttgcttgtt gacccatccg
    12481 gcatatatca tggtaaccac cttcaacatt tccgcatccg taacccgcct tccctcaatc
    12541 attttggttt ccagtcccaa gcgagtagcc agagctgttg ccgatcgccc tccaccatgt
    12601 accagtactt tgtaaccatc aatttttgaa aagtcgttca acagggaaat taaactatcg
    12661 gattcttcca caatttttcc gcctaccttg atgatggtaa gtttttcctt tttctgaaac
    12721 atatctatgt tgggcgattt atcgctattc atttctaatt cgagattatg gagctattac
    12781 ttcgatatta tgtcgaaaaa acctatatct tttattgaaa aaatcaaaac tgaatgagag
    12841 aatgcacatc ctgcacacct ttgaggcgat ctcttccctt caaatctttc agttcgatga
    12901 gaaagctcac ataaatagcc tttggtttga aattgtcgag caacgtcagc gcagcattcg
    12961 cacttccacc ggtggcgagc aaatcgtcgt gaaggagaac aacatcgtcc ttttctatgg
    13021 catctttgtg aatttcgagc gaatctactc catattcgag ttcataatcc actctataag
    13081 tagcggccgg caatttccct gctttacgaa tcagtacaaa tccggcatga agtttatacg
    13141 ccagaatgct tccaagcatg aatccccggc tttccaaacc aacaactttc gttatcccct
    13201 tgtccttgta atactcgtat agctcatcgg aaataaatct taatacttcg gcatccttca
    13261 tggccgtagt aatatcttta aaaagaactc cttttttagg gaaatcggga atattcctga
    13321 tacttgcaat aacttgttct acatccatag ttttaaaccc acacgggcaa caaaattaat
    13381 ttaaataatt gataattaaa gcaataaaat tctataaact ttcaagcatt cgtttgataa
    13441 cgacttctgc tgagatttcg cggttggcag cttcgggaat aaccaacgac tgtagacttt
    13501 cgatcacctc atcggttaca atcatattcc gccgcacagg cagacaatgc ataaaataag
    13561 cattgttggt aacggccatt tgtcggctgc tgacggtcca gcttctgtca gtgcagataa
    13621 tttttccgta gttcggatct tgataggcag cccaattttt agcataaaca aaatctgctc
    13681 cttcgaaagc tttcatctga tcgtattcta tacgtgcatt tcgcacaaat tcgggagcga
    13741 gttcataacc tttgggatga gtaataacaa attccacatc ggcttcgttc ataaaatcgg
    13801 caaaagaatt gggtacagcc tgaggcagag ctttgggatg gggtgcccac gacatgacca
    13861 ctttgggatg cggttttttg cgatattcct caatggtaat gagatcggca aaagcctgca
    13921 atggatgtac cgttgcactt tccatgctga acacaggtct tccagaatat tgaataaact
    13981 gctgaagaat ggtttcctga taatcgtacg ctttattttc aaactgcgca aaagaacgaa
    14041 cgccaattaa gtcgcaataa ttgcccatca ccggaattgc ttccagaata tgctcggttt
    14101 tatcaccatc catcaccacg ccacgttcgg tttcaagttt ccaagctccc tgatttatat
    14161 ccagcactat ggtgttcatt cccagattca ttcctgcttt ttgggtgctc agacgcgttc
    14221 tcaaactcga attaaaaaaa accatcaaca aagttttgtt tttccccaga tgctggtacc
    14281 cgaaacggtt cttcttcact tcaaaagctt cttgtaaagc ttcctgcaaa tttcccaaat
    14341 cttgtaccct gataaaagtt ttcatttcta ctatggttat ttaactgaaa tttgttaagg
    14401 tttaatagtt tcatcagaat ataatttatt tttaatgctc tgatggaacc cacatgtctg
    14461 aatattatat tcattggtgt ttatgtatgt aatacatacg ggtttcagaa aaaattaagt
    14521 atttaaaagt attttctaaa attatcaata aagaaacaga ccaaatgatg tatagaagaa
    14581 aaatgtttgc atttgattac aaaagattgt taatcttttg gttggaaaat ctgtaaacca
    14641 caatttgttt tctctgctgc acaactcaat ttatctcaag tcaatattta ttggcaaaat
    14701 tagttattat acaaataatc tcaaatgttt tattatttat tttttgtaaa ctatataatc
    14761 tattcttaga gttttattca taactgatag ttttctaact gtattaaata ttccgagagt
    14821 cattacaatc caaattttaa accgattttt gtttggttat ctcataatat attttcatgc
    14881 aatattagca tgtatttcga atatgatgct ataatttatc agtaataccg gtaaattata
    14941 ttcatttttt atacataaat atacattcta aattatatta gatgtactaa tacaattcaa
    15001 ttaattttgt cttctgaatt taaattaatt atgaagtgaa aaagtatcat gtttagataa
    15061 atatttatgt gttttttgga ttttgatata ttacttttgt tttattgatt tataacacta
    15121 aaataattaa acgtcatgaa aaaaaacaat tacttcttgt tagcagtatt tactgccgtt
    15181 gtagccttca caagccaaca agcgaaggct ttccctcctt acatttacga aggctttgat
    15241 tatgagattg gtgcaacgcc gcttaactac aatcctttag ttccgggtac ttggggtgcg
    15301 gtacatatgt ggaaacctgc atctagctgg aatggctatg gaggctcatt ttcaaacact
    15361 tttccctggg gggttactga tgtttcaccc atggtcgttc ctagtttggt tcaaacggga
    15421 aagtatttta acggcaatgg tgagtgggta tcatgtttcc ttcgtgaaat aaacaccgac
    15481 tggaattccc catattggga ttatatggat gaatcaaaat taggaaagag tggcactaca
    15541 ctttggttta gtgtaatact tcgtccgcag aagaataaga aagaagcagc gcttggatta
    15601 tgcaaagcat ataacgaaca gagtgtaatg aaaattggtg catttggtgg aaattactgg
    15661 ggcatacgtg taaataacga tgccgaacaa gcactttcga cagttcctat tgtaaacgac
    15721 gaggccaaat ttctagtgtg caaagttgaa tacacagatg ttaccggtgg aacaatcact
    15781 ttatacattg atcctactcc aggtgtggag ccatcaaatg cagcagcaac actaacaacg
    15841 acttccgata ttagattcaa atctgttcag cttcttacgc attactccgg gggttgtatg
    15901 gctgccgacg aaattcgtat tgccaccacc tatgatgagg tagcccccag tacaggacca
    15961 tcaactggtt tagtgcaaga tatgaagaaa gatattcgtg tttatagccg ggatggcaag
    16021 gttgtggcag atttaaccat gctgaaaggc ttgtcaactg tttcggttat cgacaccaaa
    16081 ggtgccgttg tgaaaaccat ccaaaatgtt ggtgctgaat ctgtaacttt tgatgtagca
    16141 aacaaaggat tatacatggt tcgtgtacag aattctggaa aatcagccac cttaaaagta
    16201 gtattgtaat ttagaaacat atttttatct tatcctgttt tccgcattgg gacaccctaa
    16261 aaatttttat aaacaatttg gtat
//
LOCUS       DAOTAX010000046        16045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3035_length_16045_cov_31.202064, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000046 DAOTAX010000000
VERSION     DAOTAX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3035_length_16045_cov_31.202064"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1429)
                     /locus_tag="PLM04_05930"
     CDS             complement(65..1429)
                     /locus_tag="PLM04_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HON02648.1"
                     /translation="MDTKGAPITLGTEKIGKLLIQYSLPAIIAMTASSLYNITDSIFI
                     GHGVGAMAISGLAVTFPFMNLSAAFGSLVGVGAATLMSVRLGQKEYGTANRILGNVFV
                     LNVIFGVAFMTVSLIFLDPILKFFGASDTTLPYAHDFMVVILIGNVFTHIYLGLNGIL
                     RSIGKPGKAMYATLLTVLINLILNPLFIFVLKWGIKGSALATVISQIVMLAWQIRHFS
                     NKNNFVHLQKGIFRLDKKIVLDSLTIGLSPFIMNAVASVIVIVINQTLIRYGGDLAIG
                     AYGIINRVSFLFVMIVMGLNQGMQPIAGYNFGAKNFNRVNEVLKLSIFIAMGMMTLGF
                     IIVEFFPHAVASLFTSDKTLIQLSIEGLRIVFVLYPMVGFQMVITNFFQSIGMSGKAI
                     FLSLTRQLLFLLPFLLVLPTFYGLKGVWYSMPAADFLATIVAAYMLFVNFGSFIKNQN
                     RDHK"
     gene            1598..2077
                     /locus_tag="PLM04_05935"
     CDS             1598..2077
                     /locus_tag="PLM04_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HON02649.1"
                     /translation="MKKTYTTDELFLKAASYCSISEHCVSEVEEKLSHWNVSENEKRK
                     IIDRLIKENFINEKRYAQSFVRDKFRLNKWGKIKIELALKSKGISNDEIKSAISTIDD
                     GEYQEMLASALKSKLSMLKYDYEYEKQGKLFRFAQSRGFETSIIDRVLRSLNQSAKE"
     gene            complement(2214..2735)
                     /locus_tag="PLM04_05940"
     CDS             complement(2214..2735)
                     /locus_tag="PLM04_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HON02650.1"
                     /translation="MNRLDAVFFASDAITVARQLLGKTLVRKWEDGTISRYKITETEA
                     YFGEEDKACHACKGKTQRTQIMYAEGGYIYVYLIYGIYWLLNVVTGNKEHPEAVLIRG
                     INNISGSGRVGKELKLDKSFYGENLISSDRIWIEDAPEALYFFTATRIGIDYADEEWK
                     SKPWRFVMTSSEK"
     gene            2981..3373
                     /locus_tag="PLM04_05945"
     CDS             2981..3373
                     /locus_tag="PLM04_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02651.1"
                     /translation="MKKILFLLFLFSNSALIGQQIYNAAGKPIGKIIGQNYYDNNSKL
                     KGKVESVFVYDAAENLIGKIDGSYLYDGNGKQIGKVENNDFYDALGTKLATVSGNALY
                     DSYGRRIRKFDGLTLSQIIVYYYYFSNL"
     gene            3496..4209
                     /locus_tag="PLM04_05950"
     CDS             3496..4209
                     /locus_tag="PLM04_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HON02652.1"
                     /translation="MLKNNIFAFIFILTFCSVFFPKVKAQGNLPYVDDKLIHFGFSLG
                     LNTADFGITPRDTLINGSRYYTDVSALQPGFSVGVITNLRLARYWNLRFNPTLHFSER
                     DITYRKDTTSTTGSTTVNSIPLSFPVYLKYSAERKDNYRPYLLGGGGVYFDFGRNKEK
                     PILLKPMDYFVEFGVGCDLYFSFFKLAPELKFAIGFNNMLTPLEQRNAGGLTEEDKIY
                     STAISKLTNKMITLSFNFE"
     gene            complement(4249..4518)
                     /locus_tag="PLM04_05955"
     CDS             complement(4249..4518)
                     /locus_tag="PLM04_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02653.1"
                     /translation="MASRKELKKQINDLTGQLALEILFRGTVMQVLEPEKMYALLSKV
                     MIMNRQFISRVNASKRIKSSKEVKDFYQQFFTDWEKNVGEIIQEF"
     gene            4648..6126
                     /locus_tag="PLM04_05960"
     CDS             4648..6126
                     /locus_tag="PLM04_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HON02654.1"
                     /translation="MFKFKKTVFFFTKLIILWAIINFFSSCQEDFTTNPADTVLFSTD
                     TLTFDTTFTSIGSATSRIMVYNPNNKAIRISSIFLASGKSSAFKINVDGKNNENNQFA
                     DIEIGAKDSLYIFVQVNVDPLDNNLPLLIEDALIFLVNGNIKTITLQAIGQDAIIFRN
                     KLIANDTTLTAEKPYLVFGYLAVDSAKTLRLDPGVKIYFHNNANLIVYGNLMADGTFE
                     KPITMRGDRFDKIKFETPVLYNVVAGQWGGIYLLWNKGKHVLHNVNLNSGYVGIYFSN
                     QDKNQLPELEISNCRIHNFLLYGLVVQNGNVKVLNSEISNSSSFCVYLNGGQHTFIHC
                     TIANYFNNSSVQPILRDKEPAVMIMDLNRIAPMRTTFLNCAIAGSMENEFTLATRFPK
                     LYKGTFSHCYIRRPNALDLPQFSNIRWSEKNDTVFKNIYFDYKKGTYFNFEPDSVSPL
                     RGLADYNIAAENCPVDLNGINRLQDGAPDAGAYEWMPAKKDE"
     gene            6168..7214
                     /locus_tag="PLM04_05965"
     CDS             6168..7214
                     /locus_tag="PLM04_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HON02655.1"
                     /translation="MKSSITSGKKRVFLATTICFLFAVGLYCQEKRNFKIVSYNVENY
                     FDCVDDSLTDDQEYLPNGIRAWNTTKYKKKQANIGKVICAIGGWESPALVGLCEVESE
                     KCLVDLTRYAGLKNLKYKFLHHESPDARGVDVALLYQPYVFKPFHDEAIRINFPQFPE
                     IKTRDILFASGIIPSGDTLHVFVCHFPSRLGGELESEERRIFVASVLRSKVDSLFSSN
                     NKANIVIMGDFNDYPPNRSLSETLKALPPTEPFLQKNLYNLMFPMHLKGKGSHKHNGE
                     WGTLDQIIVSGNLLTPTNSVYTLPSDAHIFEADFLLEDDRTFLGKQPFRTYVGMKYQG
                     GFSDHLPVYVDLWY"
     gene            7246..7968
                     /locus_tag="PLM04_05970"
     CDS             7246..7968
                     /locus_tag="PLM04_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HON02656.1"
                     /translation="MDVQLFIPCYIDQLYPQTGFNVIKILEKVGCRVHYNSNQTCCGQ
                     PAFNSGYWKETAYLAEKFLCDFNSDMPIVSPSGSCTGFILHHYNKLLANNPELLQKFK
                     EIKSQLYELTDFLVNILKTEDLGAVFPHKITFHDACSALREYGLKDEPRKLLKQVEGL
                     QLVEMDENDTCCGFGGTFAVKNHAISTSMTARKVANSLRTEAEFIVSTEASCLMNINT
                     YCKKHNLSICGIHLADVLASGY"
     gene            complement(8061..10520)
                     /locus_tag="PLM04_05975"
     CDS             complement(8061..10520)
                     /locus_tag="PLM04_05975"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HON02657.1"
                     /translation="MKKIIISILTGICCLTCIFSTSAQQVNSLYFMENSPIRSYLNPA
                     FQPTGSFYLGLPVLGYTYLSLGNNSLTLKDLVYNKNGETIWFLNPEGDVDKFYKSLRS
                     STLLNTNEQLNLLNFGFRAGSAYWTFGITEKVEGNLALPKDMFKLLLYGTPEQENNYF
                     NFKDLNANVMAYTEFALGYSKIINDQWTVGGKLKFLAGNAIVTSSNDYFNLNAGIEQW
                     NMKGTGSLNIAVPGEVTIGDKFESIDYTEPEDMQSWLKPSGMGLGFDLGFTYKPLENV
                     TLSASLIDIGTIKWNKNMKKVAYNMDYTFDGLAKIDNLDSVDMDAFLDTLSNAFKNSF
                     TTEEKATAFSTATSPKFNLGAEYAFANNKLSLGLLSTTTKVKSNMYQELTAAFNMRPS
                     NWFNLSLAYSLLNGRMSNIGAALGIRTGFFYWNLSSDYVSLDNAPLLLSDIDSSLPST
                     KIPIPYNSKGLNLALTVNFVFGNKVDKDHDGVVDKKDKCPDTPKAARKKVDANGCPLD
                     TDQDSIPDYLDQCPDTPLAARGFVDSKGCLLDTDNDSIPDYLDKCPDTPLGVAVDSLG
                     CPLDTDKDSVPDYLDRCPDTPLEAHGFIDENGCLLDTDQDGVPDYLDLCPNTPLEAIN
                     FIDKNGCPMDSDSDGVPDYLDQSPNTPLEARGLVDEKGAPRDTDGDGVYDFKDDCPKI
                     PGDSTNHGCPEVKKEVRTLFQRALKGIQFDTGKDIIKPSSYPILDQIAQVLKENPTYL
                     VEIQGHTDNVGNPQKNMELSEKRAIAVKKYLVSKGVEENRITAKGYGDTMPVASNKTA
                     AGRALNRRVEFIVTFEEVKFE"
     gene            complement(10536..12212)
                     /locus_tag="PLM04_05980"
     CDS             complement(10536..12212)
                     /locus_tag="PLM04_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02658.1"
                     /translation="MKSSASHFLVAIFLSVLILPSCMNDVDLTNISDKVQWKGSLAVP
                     IGEAALTLEDLLAQIDSQNILVTESQEILYQDSDSIVSRFRDVDLAQYSQSLVKSYYV
                     SPIVRTIPANTIFPTLNDNVIIDLGINNSPSAERVDSAVIYSANLSVKVNVENININP
                     DNLKLTLEFPTDKVRTKNGSPIKIEFTPGAFNHPENVTIRDFVINTSGNASGIPVTLK
                     LDITTGSSPVTVGPSSVVGVELKFTGFNYKVAYGYFTPAVIATKILTQDLDLPSFTDD
                     GSFLFANPKVFLTVKSNIGAYLKFNVDYVKAYPKSDSSQVVYASFNGSRSTAESLDNK
                     PAVPGLWIAKKLRTLDKDFGATDLLFSNENKPDVLEYKYSVDIDGSKNDGKPDFITPD
                     GNIKVLYKIQIPLYLKPGSYYEFKDTIPDAGEDINSALEDVDVNSGSIFLTVTNGLPV
                     KGKLTLVLKDSLGRDISTTFKKEYDIDAPQVDAEGFVVESSITPQKIEIALNNDQLND
                     FKRSKDIVYKFRIDSKDANSFIHFRKSDTFKVKLGLFVNGKVSYDSNNND"
     gene            complement(12447..13853)
                     /locus_tag="PLM04_05985"
     CDS             complement(12447..13853)
                     /locus_tag="PLM04_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HON02659.1"
                     /translation="MLSNYFVSLIKAQLPFEPNEQQNDLLEALGRFLLSSEKRKIFLL
                     KGYAGTGKTSIVGALVRALNKIGQKTVLLAPTGRAAKVFANYSGTPAFTIHKKIYRQK
                     SLTDFHFQLAENLFSDVLFIVDEASMISNTDFDSVFGSGNLLDDLMEYVFSGRNCSLM
                     LLGDSAQLPPVGQAESPALDVGILKQYRLNVFEFTLTHVVRQALKSGILHNASLIREK
                     LTNKNLVRFPPFQLEKFADISRLSPAELTDEIQKEYAGAGVEDTMVITRSNYRANYYN
                     NGIRSRVMMKEEELSNGDLLMVTRNNYFWPKYYPEIDFIANGDILEVLRIRKFTEMYG
                     FRFVDATVRSLDYDWEIDTRIWLDTLNAETPAKAQQMNKILFEKVAEDYSEIKQKRQK
                     VKKIYENEFFNALQVKFAYAITCHKAQGGQWKKVFVDPGRISDEQINDDFYRWLYTAL
                     TRATENVFLVNFPMKFFS"
     gene            13987..14571
                     /locus_tag="PLM04_05990"
     CDS             13987..14571
                     /locus_tag="PLM04_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HON02660.1"
                     /translation="MLSHLEKYQIILASKSSRRQDLFKGLNIPFEIYPIEVDESFPPE
                     LIGMDIPMYLSKKKAEACTDIMKENTMLLTADTIVWYQGQVFGKPADRNEAKFMLTTL
                     SGKTHDVITSVSITTMKKQRTFGVASEVRFARLAPEEIDFYLDNFQPYDKAGAYGVQE
                     WIGFVGVEYINGSYYNVMGLPMQRLYNELKNWKE"
     gene            complement(14584..15846)
                     /locus_tag="PLM04_05995"
     CDS             complement(14584..15846)
                     /locus_tag="PLM04_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HON02661.1"
                     /translation="MFKFLKQIDNKSYRPVFGALDIFKFIGPGLLVTVGFIDPGNWAS
                     NLAAGSNYGYSLLWMVTLSTIMLIMLQHNVAHLGIASGLCLSEAASIYLKPAYSKFVL
                     LSAMMASVSTSLAEILGGSIALNMLFDIPVRAGALLVSAFVLIMLFTNSYKVIEKWII
                     AFVSIIGLSFLYELSLVNIDWNHAVVGWVTPSFPNGSMLIIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNLTDDKTIKRQLKYEFLDTIISMLIGWAINSAMILLAAATFFETKTPVTE
                     LEQANHLLAPLLGKNASVVFAVALLFSGISSSITSGMAGGTIFAGMYKEPYDIKDNHS
                     RLGVLLSLLLAAGIIFLISNPFQGLIVSQMLLSIQLPFTIFTQVHLTSSEKVMGKYKN
                     TTFSKILLYLLGVIVSVLNILLFISFFK"
BASE COUNT         5256 a         3192 c         2787 g         4810 t
ORIGIN      
        1 attaaatcta tgcttaacat ccgttgcaaa taattcgaaa ttagtttaat cacaaagtta
       61 ttttttactt gtgatctcta ttttgattct taataaagct tccgaaattc acaaaaagca
      121 tataagccgc aacgatggta gccagaaaat cagcagccgg catactatac cagactcctt
      181 ttaatccgta aaaagttggt aatacgagca gaaaaggcaa caagaacaac aattgtcggg
      241 taagtgacag gaaaatggct ttgcctgaca ttccgatact ttggaaaaag ttggtgataa
      301 ccatttgaaa acctaccatc ggatacaaaa caaatacgat tctcaatcct tctatcgaca
      361 attggataag agttttgtcg cttgtgaaaa gagaagccac agcatgagga aaaaactcta
      421 caataatgaa gccgagagtc atcattccca tagctatgaa aatgctgagc tttaaaactt
      481 catttactct attaaaattt ttagctccaa aattatatcc ggctatcggt tgcattccct
      541 gattcagtcc cattacaatc atgacaaaaa gaaaactgac ccgattaata attccatacg
      601 ccccaatagc caaatcgcca ccatagcgta tcaaggtctg attgataaca attacaatca
      661 cgctggcaac agcattcatt atgaaaggag ataagccaat ggttaacgaa tcgagtacaa
      721 tttttttatc caaccggaaa attccctttt gcaaatgaac aaaattattc ttgttgctga
      781 aatgacgaat ttgccaagca agcattacta tttgcgaaat aactgtcgcc agagcgcttc
      841 ctttgatacc ccattttaaa acaaagataa ataacggatt taaaatcaaa tttatcaata
      901 ccgtcagcaa agtcgcatac atggcctttc ccggtttgcc tatcgagcgc aaaataccgt
      961 ttaacccaag ataaatatgt gtaaatacat tccctataag tatgactacc ataaaatcgt
     1021 gtgcataagg caaagttgta tcgctagcgc caaaaaattt cagaatagga tcgagaaaaa
     1081 ttaaagaaac cgtcatgaac gccactccaa aaataacatt taacacaaag acatttccca
     1141 aaattctgtt tgctgtgcca tattcctttt gtcccaacct taccgacatg agtgttgcag
     1201 ctccgacgcc taccagcgat ccgaaagccg ccgacaaatt cataaacggg aaagttactg
     1261 ccaatcccga tatagccatt gcacctacac catgtccaat gaaaatactg tcggttatgt
     1321 tgtagagcga tgatgcagtc attgcaatga tggcaggcaa agaatattgg ataagtaatt
     1381 ttccaatttt ttctgttcct aatgttatgg gtgctccttt tgtatccatt tttaaatttt
     1441 taggaatgca aatttacaaa ttatgtgctt gttttccttc atttattttt gaaaataccc
     1501 tttcgtctga tatagaaaaa accatgaaaa cagtccgatt tttttgaaaa ttattatttt
     1561 tgtattcctt tatcatttac aaaaaaatct ttttatcatg aagaaaacgt ataccaccga
     1621 tgagcttttt ttaaaagcag catcctattg ttcaatttct gaacattgtg tttctgaagt
     1681 ggaagaaaaa ctaagccatt ggaacgtttc agaaaatgaa aaaagaaaaa taattgatag
     1741 attaataaaa gaaaatttta tcaatgaaaa acgttatgcc caatcgtttg ttcgggataa
     1801 atttcgatta aataaatggg gaaaaatcaa gattgaattg gctttgaaat caaaaggaat
     1861 atctaacgat gaaataaaat ctgcaatttc taccattgat gatggagaat atcaggaaat
     1921 gcttgcttca gcacttaagt caaaactatc aatgctcaaa tacgattacg aatatgaaaa
     1981 acaaggtaaa ctttttcgat ttgcccaaag tcgaggtttt gaaacatcca ttatcgatcg
     2041 tgttttgcgt tcactgaacc aatccgccaa agaatagtca gaaaaggtaa aaaaaatttt
     2101 aagttgcaaa acatatcaaa taaaaacctt cttaaaaatt ataataacag atctctttac
     2161 aataattgag ttgttccaaa tctccattat caaatcaccg gggaaaagaa attttatttt
     2221 tcggatgatg tcattacaaa ccgccatggc tttgatttcc attcttcatc ggcataatcg
     2281 atcccgatgc gcgtagccgt aaaaaaatat aacgcttcgg gagcatcttc aatccagatt
     2341 ctatctgaag agattagatt ttcgccataa aaacttttat caagttttaa ttcttttccc
     2401 actcttcctg atcccgaaat attgttaatt ccccttatca gtactgcttc cggatgttct
     2461 ttgttgcctg taaccacatt cagcaaccaa taaatcccat aaatcagata aacataaatg
     2521 taacctcctt cggcatacat aatctgtgtt cgctgagttt ttcccttgca ggcatgacaa
     2581 gccttatctt cttctccaaa ataagcttcc gtttctgtta ttttgtatcg ggaaattgta
     2641 ccatcttccc actttcttac taaagttttt cccaatagtt gacgggcaac cgtaatcgca
     2701 tcggaagcaa aaaaaaccgc atccagtcga ttcataaaaa attataaact caaaaaaatt
     2761 aatttgttgt cggtttacac tgcatttaat gcaaatttac atcaaatatc tcaattaaca
     2821 actaaaattt cagtgtagta tctaactccc attgaaaaaa caactttgaa taaaacaaca
     2881 cctaaattca aattttttct gaagcagaat aaaaaatgac tcattatttg ttttttcaat
     2941 ttattttcaa ttaatttgta ttcactaaaa gtcttttgag atgaaaaaga tactatttct
     3001 tctgttcctt ttttcaaatt ctgcattaat aggacaacaa atctacaatg ctgcagggaa
     3061 gcctattgga aaaattattg gacaaaatta ttacgacaat aattcaaaat tgaaaggaaa
     3121 agttgaaagc gtttttgttt atgacgcagc agaaaacctg atagggaaaa tcgacggaag
     3181 ttatctttac gatggcaacg ggaaacaaat tgggaaagtc gaaaataacg atttttatga
     3241 tgcactcgga acaaagttgg ctacagtttc cggaaatgct ttatatgaca gctatggacg
     3301 aagaattcgg aaatttgatg gtttgacact cagtcaaatc attgtctatt attactattt
     3361 ttccaatttg taaaatttct tgccttttct tctttattat ttgttctcaa tcctcgctcg
     3421 ttaaaccggc aataatatca acttttaagc gttttttata gcaaagccca acgtgaaaat
     3481 tcttaggaaa tcagtatgtt aaaaaacaat atttttgctt ttatttttat cttaactttt
     3541 tgttcggtgt tttttcctaa agttaaagct caaggaaatt taccttacgt agatgacaaa
     3601 ctcattcatt tcgggttttc tttaggattg aatacggctg attttggaat aacgccaaga
     3661 gatacactta ttaatgggag caggtattac acagatgtat ctgccttgca acccggattt
     3721 tcggttggtg tgataaccaa tcttcgattg gcacgatatt ggaatctgcg cttcaatcca
     3781 actttacatt tttccgaaag agacatcact tatcggaaag ataccacaag tacaactggc
     3841 tccacaactg tcaactctat tccactgagc ttccccgtat atttaaaata cagtgctgaa
     3901 aggaaagaca attatcgtcc ctatctgttg ggaggcggag gagtgtattt tgattttgga
     3961 agaaacaagg aaaaacccat cttactaaaa ccgatggatt attttgttga atttggcgta
     4021 ggatgtgatt tatatttcag ttttttcaaa ttggcacccg aattaaaatt tgccattgga
     4081 ttcaacaaca tgcttacgcc tctcgaacaa agaaatgcag gaggattaac cgaagaagat
     4141 aaaatttatt ccactgccat ttccaaactt accaataaaa tgataaccct ttcttttaat
     4201 ttcgagtaag tttcaaaatg tcagagtgca taaccgctga aagaaaaatt agaactcttg
     4261 aataatttct cccacattct tttcccaatc ggtaaaaaat tgttgataga aatctttcac
     4321 ttcttttgaa ctttttatcc tttttgatgc atttaccctt gaaataaact gcctgttcat
     4381 gatcataact tttgatagca aagcatacat tttttcaggt tccaaaacct gcataacagt
     4441 gccacgaaat aaaatttcca atgccaactg acccgtaaga tcatttattt gttttttcaa
     4501 ttcttttctg cttgccatga tagaatgatt taagtttttt gttgcacaaa tataaaccaa
     4561 tattatgata aagtcatctt ttacattaaa tttgcaaaaa caaatccagc ttaacatcca
     4621 tttaaaacag caaaaaccaa cgatagaatg ttcaaattta aaaaaacagt tttcttcttc
     4681 acaaaactga ttattctttg ggcaataatt aattttttct cttcatgcca ggaagatttt
     4741 actaccaatc ccgcagatac tgttctattt tcgacggaca cattgacatt cgacaccacc
     4801 tttacatcca ttggtagtgc aacttccaga attatggttt acaatcccaa taacaaagcc
     4861 atacggattt cgagcatttt tttggcaagc ggtaaatcat cggcctttaa aatcaatgta
     4921 gatggtaaaa acaatgaaaa caatcagttt gccgatatag aaattggcgc aaaagacagc
     4981 ctgtatattt ttgttcaagt caacgtcgat ccacttgaca acaatttgcc cttactgata
     5041 gaagatgcat taatttttct ggtaaatggc aatataaaaa caattacctt gcaggccatt
     5101 ggacaagatg caatcatttt cagaaataaa ctcattgcaa acgacacgac actgacagcc
     5161 gaaaagcctt atctcgtttt cggatacctc gccgtcgaca gtgcaaaaac actgaggctg
     5221 gatccgggag taaaaatata ttttcacaac aatgccaatc ttattgttta tggaaattta
     5281 atggctgacg gaacttttga aaagccaatc accatgcgtg gagataggtt tgacaaaata
     5341 aaatttgaaa cgcccgtatt gtacaatgtc gttgccgggc aatggggagg aatttatttg
     5401 ctttggaata aaggaaaaca tgttctgcac aacgtaaatt taaatagtgg ctacgttgga
     5461 atttattttt ccaatcagga taaaaatcaa ttaccggaac ttgagatttc aaactgtcgg
     5521 atacacaact ttttgcttta cggactggtg gttcaaaacg gcaatgtgaa ggttttgaac
     5581 tcagaaattt ccaacagcag cagtttttgc gtttatctca acggtggaca gcatactttc
     5641 attcattgca cgatagcaaa ttattttaac aacagttcag ttcagccaat tctgcgcgac
     5701 aaagaaccgg ccgttatgat tatggattta aaccgcattg cacccatgcg gactactttt
     5761 ttaaattgtg caatagccgg cagtatggaa aacgaattta ctcttgccac ccgtttccct
     5821 aaattgtaca aaggaacttt ttcccattgt tacattcgta ggcccaatgc cttagacttg
     5881 ccccaatttt ccaatatccg ctggtcagag aaaaacgata ccgtttttaa aaatatttat
     5941 tttgattaca aaaaaggaac ttatttcaat ttcgaaccgg attctgtttc tcctttaaga
     6001 ggcttggccg attacaatat tgctgccgaa aactgtcctg tcgatttaaa tggaattaac
     6061 cgtttgcagg atggagcacc cgatgcaggc gcttacgaat ggatgccggc gaaaaaggat
     6121 gaataaatta ttcaaggtta aaaaattttc aaagaaacat cttcaccatg aagtcctcga
     6181 ttacctccgg taaaaaaaga gtttttttag caacaacaat ttgttttctt tttgctgtag
     6241 gactttattg tcaagagaaa cgaaacttta agattgtttc ttacaatgtt gaaaattatt
     6301 tcgactgcgt ggacgattcg cttactgacg accaagaata cctgccaaat ggaataagag
     6361 catggaatac aacaaaatat aaaaagaaac aagccaacat cggtaaagta atctgcgcca
     6421 ttggtggctg ggaaagtccg gcattagttg gactatgcga agtggaaagc gaaaaatgtc
     6481 tggtagactt gacccgttat gccggcctga aaaatttaaa atacaaattc ctccatcacg
     6541 agtcgcctga tgccagaggc gtggatgttg cacttcttta ccaaccttac gtatttaaac
     6601 cttttcacga tgaagccata cgcataaact ttccgcaatt tcccgaaatt aaaacacgcg
     6661 atattttgtt tgcttcagga ataattcctt cgggagatac actacatgtc tttgtgtgcc
     6721 attttccttc gaggctggga ggcgaattgg aatcggaaga aagaagaata tttgtagctt
     6781 ccgtattgcg ttcaaaagta gacagtttgt tttcgtccaa caataaagca aatattgtta
     6841 tcatgggaga tttcaacgat tacccgccaa atcgaagtct gtcggaaacg ctgaaagctc
     6901 tgcctccaac ggagccgttt ttacaaaaaa atttgtacaa tttaatgttc ccgatgcatt
     6961 tgaaaggaaa aggctcgcac aaacacaatg gagaatgggg gacactcgac caaatcattg
     7021 tttcaggaaa tttgttgacc ccaaccaatt cggtttatac tcttccaagt gatgcgcaca
     7081 tttttgaagc cgatttttta ctggaagatg accggacatt tttaggaaaa cagcctttcc
     7141 gaacttacgt aggcatgaag tatcagggtg gtttttccga ccatcttcct gtttatgtcg
     7201 atttatggta ctgattcaaa aaattttttc taaattttta catacatgga tgttcaatta
     7261 ttcattcctt gttatattga tcaactttat ccgcaaacag gttttaacgt tataaaaatt
     7321 cttgagaaag tcggttgtcg agttcactac aattccaacc aaacctgttg tggacaacct
     7381 gcattcaaca gtggttattg gaaagaaaca gcttatttgg cagaaaaatt tctttgcgat
     7441 tttaattcgg atatgcctat agtttctcct tccggctcct gcaccggatt tattttgcat
     7501 cattataaca aattacttgc gaataacccc gaactactgc aaaaatttaa agaaatcaaa
     7561 agtcagttgt acgaattaac cgattttctg gtaaatattt taaaaacaga agatttaggc
     7621 gctgttttcc ctcacaaaat aacttttcat gatgcttgct cggccttacg tgaatatgga
     7681 ttgaaggatg agccccgaaa actgctcaaa caggtagaag gactgcaatt ggttgaaatg
     7741 gacgaaaatg atacgtgttg tggttttgga ggcacatttg ccgtaaaaaa tcatgccatt
     7801 tccacttcca tgacagcacg aaaagttgcc aacagtctta gaacagaagc cgaatttatt
     7861 gtgagcacgg aagcctcatg ccttatgaat atcaatactt actgcaaaaa acataattta
     7921 tccatttgtg gcattcatct ggccgatgta ctggcgtcag gatattaaaa acttatttgc
     7981 ctaaaaattt taaggcactt caaactttac gattgaaatg ccttaaaaat aatcgaacaa
     8041 ctataaaaga aaaatattcc ttattcgaat ttcacttcct caaaagttac aataaactct
     8101 actcttctgt ttaatgctct gcctgcagca gttttgttgg acgcaacggg catggtgtct
     8161 ccatagcctt ttgccgttat cctgttttct tccacacctt tagaaacaag atattttttc
     8221 acggctatag cccgtttttc tgaaagttcc atatttttct gaggattacc tacattatcg
     8281 gtatgtccct gaatttctac caaataggtc ggattttctt tcaatacttg agcgatttgg
     8341 tccaaaatgg gataagaaga aggtttgata atatcttttc cggtatcaaa ctggatgcct
     8401 tttaatgctc tttggaaaag tgtgcgcacc tcttttttca cttcaggaca accatgattg
     8461 gtgctgtcgc cggggatttt tggacaatca tccttgaaat cataaacgcc atcaccatcg
     8521 gtatcgcggg gagcaccttt ttcatctacc aatcctcgag cttctagagg agtattaggt
     8581 gactggtcca aataatcggg aacgccatcg ctatcgctat ccattggaca gccattttta
     8641 tcaatgaaat ttattgcttc caatggtgta ttcggacata aatccagata atcgggaact
     8701 ccatcctgat ccgtatccaa taagcaacca ttttcatcaa taaacccgtg agcttcaagt
     8761 ggtgtatcgg gacaccgatc aaggtaatcg ggaacactat ccttgtccgt atccaatgga
     8821 caacccaaag aatctacggc tactcccaat ggtgtgtcgg gacatttgtc gagataatcg
     8881 ggaatgctgt cgttatcggt atccaaaagg caacccttgc tatccacaaa accacgagca
     8941 gccaatggcg tatcgggaca ttgatccaga taatcgggaa tactatcctg atccgtatcc
     9001 agcggacagc cgtttgcatc cactttcttg cgtgctgctt tgggtgtgtc gggacattta
     9061 tcctttttgt ccaccactcc atcatgatct ttatcgactt tgttaccaaa aacaaaatta
     9121 accgtcaatg ccaaattcag ccctttactg ttgtaaggaa ttggaatttt ggttgatggc
     9181 aaggaagaat caatatcgct caataacaaa ggtgcattat ctaacgaaac atagtcgctg
     9241 gaaagattcc aatagaaaaa tccggttctt attcccaaag ccgctccaat attgctcatt
     9301 ctcccattca acagagaata ggcaagagat aagttgaacc aattacttgg acgcatattg
     9361 aacgcagctg tcagctcctg atacatgttg cttttcactt tggtggtagt agaaagtaaa
     9421 cccagactga gtttgttgtt tgcaaaagca tattctgcac ctaaattgaa ttttggagaa
     9481 gtagcagtag agaatgccgt tgctttttct tcggttgtga aggaattttt aaaggcattg
     9541 gacaacgtat ccaaaaatgc atccatatct acagaatcca aattgtctat ttttgcaagt
     9601 ccatcaaagg tataatccat attgtaggct acctttttca tgtttttgtt ccacttaatc
     9661 gttcctatgt caattaagga tgcggaaaga gttacatttt ccaaaggttt atatgtaaat
     9721 cccaagtcga accctaaacc catacccgaa ggcttcagcc aactctgcat atcttccggt
     9781 tcagtataat cgatagattc aaatttatcg ccaatcgtta cttctccggg aacagcaata
     9841 ttcaaagaac ctgttccttt catattccac tgttcgattc cggcattcaa attaaaataa
     9901 tcgttggaag aagttacaat ggcattcccg gccaaaaatt tcaattttcc gcccactgtc
     9961 cattggtcat taattatttt ggaatacccc agcgcaaatt cggtgtaagc cataacattg
    10021 gcattcaaat ccttgaaatt gaaataattg ttttcttgtt cgggtgttcc ataaagcaat
    10081 agtttaaaca tatccttagg caatgccaaa tttccttcta ctttctcggt aattccgaat
    10141 gtccaataag cactgccggc acgaaagcca aagtttaaaa gattcagttg ttcgttggta
    10201 ttgagcaacg tcgaggaacg caaagattta taaaatttat cgacatcacc ttccggattt
    10261 aaaaaccaga tggtctctcc atttttattg taaaccaaat ccttcagcgt caacgaattg
    10321 tttcccaaac tcaaataagt ataacccaac acgggtaatc ccaaataaaa actaccggtt
    10381 ggttgaaaag ccggattcaa ataagaacgg atgggcgaat tttccataaa atataacgaa
    10441 ttcacctgct gagctgatgt agaaaagatg caggtcaagc aacatattcc tgtcagtatt
    10501 gaaataataa tctttttcat ggttattcgg ttttattaat cgttgttatt tgaatcataa
    10561 ctaacttttc catttacaaa caaaccaagt tttaccttga aggtatccga ttttctgaaa
    10621 tgaataaagg aattggcatc tttgctgtcg attctgaatt tataaacaat atcttttgaa
    10681 cgtttgaagt cattcagttg atcgttgttt aaggcgattt ctattttttg aggggtaatg
    10741 ctgctttcca ccacaaaacc ttcagcatct acttgcggag catcaatatc atattctttt
    10801 ttaaaggtag tagaaatatc tcttcccaat gaatccttca aaaccaacgt taatttgcct
    10861 tttacgggaa gtccgttagt aacggtgagg aaaatcgatc cactattaac atctacatcc
    10921 tccaaagccg aattaatatc ttcaccggca tcgggaatgg tgtccttaaa ttcgtaatag
    10981 ctgcccggtt tgagatataa tggaatctga atcttataca aaactttaat gttaccgtcg
    11041 ggcgttataa aatcaggttt accatcattt ttgcttccgt caatgtcaac gctgtattta
    11101 tattccaata catcgggttt gttttcatta ctgaaaagca aatcggtagc accaaaatcc
    11161 ttgtctaaag ttctaagttt tttagcaatc cataaacccg gaactgccgg cttgttatcc
    11221 aacgattcgg ctgttgaacg acttccattg aaagatgcat aaaccacttg agaagaatcg
    11281 gatttgggat aggctttcac ataatccaca ttaaatttca ggtaagcccc gatattgctt
    11341 ttaacggtaa gaaaaacttt gggattggca aacaaaaacg agccatcatc cgtgaaagaa
    11401 ggtaaatcca aatcttgtgt cagtattttg gttgcaatta ctgccggtgt aaaataaccg
    11461 taagcaacct tatagttaaa accggtaaat ttcagttcta cgccaaccac cgaacttggt
    11521 cctacagtaa cgggagaact gccggtggtt atatccaact ttaaagttac cggtattccc
    11581 gacgcatttc cggaagtatt gattacaaaa tctctgatgg ttacattttc aggatggtta
    11641 aaagctccag gtgtaaattc aatttttatc ggggagccgt tcttggtacg caccttgtca
    11701 gtggggaact ccagcgtcag tttcagatta tcgggattaa tgttgatatt ttccacatta
    11761 accttcaccg acaaattggc cgaatagata actgcactgt caactctttc agcggaagga
    11821 gagttgttta tgcccaaatc gataattaca ttatcattta aagttgggaa aattgtgtta
    11881 gcaggaatag ttctaactat tggagaaaca tagtaagatt ttaccagtga ttgggaatac
    11941 tgagctaaat caacatccct gaaacgggaa actatactgt cggaatcctg ataaagaatt
    12001 tcttgacttt cggtaacaag aatattctga ctgtcgattt gagccagcaa atcttccaat
    12061 gtcagagcgg cttctccaat aggtacagcc aaagaaccct tccattgaac cttatcggag
    12121 atgttggtaa gatccacatc gttcatgcat gagggcaaaa tcaaaacgct taaaaagatt
    12181 gccacaagaa aatgggaagc tgaagatttc attttaattg aatattaagg ttacacaatt
    12241 gaaaaagtaa aatcaaataa taaacagcta acagggaaaa attaaatcaa aaaaatgttt
    12301 agaatgttga caaagagcgc aaacaaacag tacagcagcc tcaaaagcaa aaatgaaatt
    12361 tagaataaat ttaaaagatt ttataatttt tatattcttc acaaaagtaa ttaaaagttg
    12421 gaaaatgcaa aaaaattctc tgttttttac gagaaaaatt tcatgggaaa atttaccaga
    12481 aaaacatttt cggtggcgcg ggtcaaggcc gtataaagcc atcggtaaaa atcgtcattg
    12541 atttgttcgt cggaaatcct tccgggatcg acaaaaacct ttttccattg accgccctgc
    12601 gctttatgac acgtaatggc ataagcaaat ttcacctgaa gggcattgaa aaattcattt
    12661 tcataaattt tcttcacttt ctgccgtttt tgcttaattt cagaataatc ttccgccact
    12721 ttttcaaaca atatcttatt catttgctgc gcttttgccg gtgtttctgc gttgagggta
    12781 tccagccaaa tgcgggtatc aatttcccaa tcataatcga gagagcgcac agtggcatcc
    12841 acaaatcgaa aaccatacat ttcggtaaat tttctgatac gcaaaacttc cagtatatcg
    12901 ccgtttgcta taaaatctat ttcgggataa tattttggcc agaaataatt gttacgcgta
    12961 accatcagca agtcaccatt cgacaactcc tcttctttca tcatcacccg cgagcgaatg
    13021 ccattgttgt aataattagc cctgtaattg gaacgcgtaa tgaccatcgt atcctccact
    13081 cctgcgccgg catattcttt ctgtatttca tccgtcaatt cggccggact taagcgggaa
    13141 atatcggcaa atttttccaa ctgaaaagga ggaaatcgaa ctaaattttt atttgtcagc
    13201 ttttccctta tcaaagaagc attatgcaaa atgccacttt tcagcgcctg acgaacgaca
    13261 tgggtcaatg taaattcaaa aacattcaat cggtattgtt tcaatatacc aacatccaat
    13321 gccggacttt cggcttgtcc taccggtgga agctgcgccg aatcgcccaa cagcatcaag
    13381 ctgcagttgc gtccgctaaa aacatattcc atcaaatcgt ccaacaagtt acccgaacca
    13441 aaaaccgaat cgaaatcggt attggaaatc atcgaagcct catccacaat aaaaagtaca
    13501 tccgagaaaa gattttccgc caactgaaaa tggaaatcgg tcaaggattt ttgacgataa
    13561 attttcttgt gtatcgtaaa agccggcgtt cccgaataat ttgcaaaaac ttttgccgcc
    13621 ctgcccgttg gagccagtaa aactgtcttt tgcccaattt tatttaacgc cctgaccaaa
    13681 gcacccacaa tcgaagtttt accggttccg gcataacctt tcaacagaaa aatttttctt
    13741 ttttcactgg atagcagaaa ccttcccaat gcttcaagca aatcattttg ctgttcattc
    13801 ggttcaaaag gcaactgcgc ttttatcagc gaaacaaaat agttacttaa catacggcaa
    13861 atttaaacga tatttttgca aaatatgctg cacaacaaaa aaaaatcata tttttgtagc
    13921 aataatgtcc ggagtaattc ggtaccagaa caaaaacaat ccttcaaacc gaaaaaaatg
    13981 ttcaaattgc tttcacattt ggaaaaatac caaattattc ttgcctcaaa atcatcaaga
    14041 agacaggatt tatttaaagg attgaatatc ccttttgaaa tttatccgat tgaagtagat
    14101 gagtcttttc ctcccgaact tatagggatg gacattccca tgtatctatc gaagaaaaaa
    14161 gcggaagctt gcaccgacat tatgaaagaa aataccatgc tgcttacagc cgataccatt
    14221 gtttggtatc agggacaagt tttcggaaaa cccgccgacc gaaacgaagc taaatttatg
    14281 ttaaccacgc tgtcgggtaa aacgcacgat gtcatcacca gcgtcagcat tactaccatg
    14341 aaaaaacaac gaacctttgg cgtggcttct gaagttcgtt ttgcacgatt ggcacctgaa
    14401 gagattgatt tttatctcga caattttcaa ccctacgaca aagccggcgc ttatggagta
    14461 caggaatgga ttggctttgt aggagttgaa tatattaacg gctcctacta caacgtaatg
    14521 ggtctaccca tgcaacgatt gtacaatgag ctgaaaaact ggaaagaata aaaaccaaaa
    14581 atcttacttg aaaaagctga taaacagtaa aatattcagt acggaaacaa taacgcccaa
    14641 caagtaaagc agtatttttg aaaaagtagt atttttatat ttccccatta ctttttctga
    14701 tgaagttaag tgcacttgcg taaaaatagt gaaaggtaat tggatactca ataacatttg
    14761 agagacaata agcccttgaa atgggttgga aatcaaaaag ataatacctg ctgccagtaa
    14821 aagtgataaa aggacaccca gtcgcgaatg gttgtcttta atatcgtaag gttccttgta
    14881 catgccggca aaaattgttc ctccggccat accggaagta atggaagagg aaatacctga
    14941 aaacaacaat gcaacggcaa aaacaacaga agcattttta cccagcaacg gagccagcaa
    15001 atgattggct tgttccaatt cggtaaccgg cgttttcgtt tcaaaaaaag tggcggcagc
    15061 cagcaaaatc atggcactgt tgatagccca accaataagc atggaaatta tagtatcaag
    15121 aaattcgtat ttcaactgcc gtttgatggt tttatcatcg gtcaaattcc attggcggct
    15181 ctgaatcact tccgaatgca aaaacaaatt gtgtggcatg accactgctc ccaacacgct
    15241 cataataatt aacatggagc catttggaaa acttggagtt acccagccca caacagcatg
    15301 attccaatct atatttacca acgacaattc atacaaaaat gaaagtccaa taatggagac
    15361 aaaagcaatg atccattttt cgataacctt ataagaattg gtaaacagca taataagtac
    15421 aaaagcagaa accagcaaag caccggctcg aacgggaata tcgaacagca tgttcaaagc
    15481 aatgcttcct cccaaaattt cggccaaaga agtagaaaca gaagccatca tggccgaaag
    15541 caggacaaac ttggaatacg ctggtttcag gtaaatagaa gcggcttcgg acaagcacaa
    15601 accggatgca atgcccaaat gcgctacatt gtgctgcaac ataataagca ttatggttga
    15661 caaagttacc atccacagca acgaataacc gtaattcgaa ccggcagcca gattagaagc
    15721 ccaattgccc ggatcgataa aaccaaccgt taccagtaaa ccggggccga taaatttaaa
    15781 aatatccaat gccccaaata ccggacgata agatttattg tcgatctgct tcaaaaactt
    15841 gaacacgctt cgattctttt tattggttga aaaactggac aaaaatatca aattttgttt
    15901 aaacaaccat ttaccgtgaa ttatttattt tacgctttaa ttttatttac aaatgattta
    15961 atatcatagt ttgataaaaa tatatcacaa atagaagtgg ctttttagtt atttttgcaa
    16021 tacgtgatta tacatgattt aatta
//
LOCUS       DAOTAX010000047        15639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3152_length_15639_cov_31.269764, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000047 DAOTAX010000000
VERSION     DAOTAX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15639
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_15639_cov_31.269764"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1526)
                     /locus_tag="PLM04_06000"
     CDS             complement(216..1526)
                     /locus_tag="PLM04_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HON02662.1"
                     /translation="MSISKDLVDEIFRQFGQEDINKLSIREMGKLVAQIEQKTNVEFI
                     HMEMGVPGLPPLQTTVDAEKEALDRGLAALYPDIEGIPFVKNETARFAKLFMDIDVTP
                     LNCIPTVGAMQGSFAALLAAARTNKNKPCTLFIDPGFPVNKSQLQLMGLPFKSFDILE
                     NRGERLRSALTSFLKEGDICTITYSSPNNPSWINFTEEELQIIGELATEYDVIVVEDL
                     AYFGMDFRKDYGRPGVPPYQPTVARYTENYILLLSASKIFSYAGQRIGMMIISESLRT
                     RHYPELKNYFTSDVLGHFIPYGALYATTAGTSHTAQYAFAATLKAVNDGKINFLEALK
                     EYAEKAKIMKEIFLKNGFYIVYKEKNEELADGFYFTVAYPEMTSSQLMKNFLYYGLSA
                     ISLSITGSKDNHGLRICVSFVRPDQIPVLDKRLKEFAEDMKKNV"
     gene            complement(1546..2844)
                     /locus_tag="PLM04_06005"
     CDS             complement(1546..2844)
                     /locus_tag="PLM04_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HON02663.1"
                     /translation="MIWNEEIECMSSSEMRKLQSERLKELVNKVYQNVEFYRKKMDEK
                     GVRPSDIRSIDDISKLPFTNKTDLRDNYPFGLFAAPMNEIVRIHASSGTTGKPTTVGY
                     TKKDIENWQEVLARCLIMSGVTPEDIVQVSFGYGLFTGGLGIHYGAENVGCTVVPISA
                     GNTRRQLQLMSDFGSTVLACTPSYALHLADAMSEFGYSLSDMKLKTGVFGAEPFSENM
                     RLELEKKWGIKAFDIYGLSEIMGPGVANDCEFHDGLHIHEDHFFPEVVDPKTKEPLPD
                     GQQGELVFTTLTKEGIPLLRYNTHDLTTLNRERCECGRTLVRMKKITGRSDDMLIIRG
                     VNLFPSQIEQVLLEMGETSAHYMLYVDRENNLDILELKVELDETKMVDTIKDLMSLSR
                     KIEHSLHSALGLSVKVTLVEPKSIPRSEGKAVRVIDNRKI"
     gene            complement(2841..3269)
                     /locus_tag="PLM04_06010"
     CDS             complement(2841..3269)
                     /locus_tag="PLM04_06010"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HON02664.1"
                     /translation="MKIRQLSVFVENKTGHLNQILAVLARNNINIIALTLADSIDFGL
                     LRMLVSDAEKAAEALRTENFTVRIHEVLSLELEGVPGSLSRVLNHFSDNGIAIEYIYA
                     FSFGSKSILVMRPDNAEKAIEIIQKYQLKPIYESDLKAQL"
     gene            3462..4457
                     /locus_tag="PLM04_06015"
     CDS             3462..4457
                     /locus_tag="PLM04_06015"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HON02665.1"
                     /translation="MFPYPLLHRNELIRQMNNLGKSNTPFFFLLDFMAQNGYIIKNQD
                     LDDQYIRFQLSDESFSNQEKSTKNSFNFQWNIHPVNFDEYQLKFHFVMENIRCGNSFL
                     LNLTQPTEVSTNLNLLELYELSVSKYKLWLKDLFTVFSPETFIKIEDGIISTFPMKGT
                     IDASVANAEDAILNDFKEQAEHATIVDLMRNDLSMVAEKVEVKKYRYIDKIITNQNEL
                     FQTSSEITGELPENYCCYLGNIIISMLPAGSISGAPKRKTLEIIREIEGYDRGFYTGI
                     FGQYDGKNLDSAVLIRFIEQKNGQMIFKSGGGITSNSEVLKEYREMLEKVYVPIY"
     gene            4441..5037
                     /locus_tag="PLM04_06020"
     CDS             4441..5037
                     /locus_tag="PLM04_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HON02666.1"
                     /translation="MYRFIESIKLLDGIFYRLKFHQKRMNICSELFYPNDDFIHLQSA
                     LTDSHPPQKGLFKCKITFESKILKIEYLPYKKKEINSLRIVHTEIESRPYKKEDRTDF
                     NAAFDMRGNYDDVLLMKNGLLTDTSICNIALFDGFSWQTPTKPLIYGVNRANLLEEGK
                     IFEKDIKLSDLQNYSAIRLFNALIEFGEIELNISNIHF"
     gene            5375..6868
                     /locus_tag="PLM04_06025"
     CDS             5375..6868
                     /locus_tag="PLM04_06025"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HON02667.1"
                     /translation="MDRLFIFDTTLRDGEQVPGCQLNTVEKIEVAKALENLGVDIIEA
                     GFPVSSPGDFNSVVEISKTVTWPVICALTRAVEKDIEVAAEALQYAKKKRIHTGIGTS
                     EYHIRYKFNSNQEEILERSIAAVKYAKKFVEDVEFYCEDAGRTENEYLARVVEAVIKA
                     GATVVNIPDTTGYCLPWEYGEKIKYLMEHVDGIHKAIISTHCHNDLGMATANTISGIM
                     NGARQAEVTINGIGERAGNTALEEIAMILKCHKKLNIETQINTKKIYSTSRLVSNLMN
                     MPVQPNKAVVGRNAFAHSSGIHQDGVLKNRENYEIMNPEDVGIDHNSIVLTARSGRAA
                     LKHRLNTLGVELADKKLDAVYEEFLKLADKKKDINDDDLLMLAGKERSEKERIKLDYL
                     QVTSGVGVKPVASIGLIIGSEKFEAASAGNGPVDAAIKALKKIINREMTLQEFTIQAI
                     NKGSDDVGKVHMQVEHEGVVYYGFGANTDIVAASVEAYIDAINKFVE"
     gene            7028..8428
                     /gene="leuC"
                     /locus_tag="PLM04_06030"
     CDS             7028..8428
                     /gene="leuC"
                     /locus_tag="PLM04_06030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HON02668.1"
                     /translation="MNTLFDKIWDAHVVSTIENGPTILYIDRHYVHEVTSPQAFNGLR
                     ARGLKVFRPLQTTLTADHNIPTLHQDQPVKDPVSRNQLDTFEKNARDFQLPLFFPMGH
                     EKNGVVHIIGPENGFTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLATQCILQ
                     TRPKTMRITFDGKLNKGVTAKDLALYMISKLSTGGATGYFVEYAGEAIRNMNMEERMT
                     VCNLSIEMGARGGMIAPDETTFAYLKNREFAPKGEEWNKKLAYWKTLYSDQDAVFDKE
                     YTFDASEISPMITYGTNPGMGMGIDQTIPTLEQVDEAGRISFEKALNYMGFRPGEKLL
                     GKPIDYVFLGSCTNGRIEDFRTFAQFVKGKKKADNVVAWLVPGSRKVEKQIHDEGLHE
                     ILMEAGFELRQPGCSACLAMNDDKVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAA
                     AAAVTGKITDPREFLS"
     gene            8519..9109
                     /gene="leuD"
                     /locus_tag="PLM04_06035"
     CDS             8519..9109
                     /gene="leuD"
                     /locus_tag="PLM04_06035"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HON02669.1"
                     /translation="MEKFVKLTSTVVPLPIENVDTDQIIPARFLKATNKDGFGDHLFA
                     DWRYNKDGSPKADFVLNNPTYKGSILVAGKNFGSGSSREHAAWAIAGYGFKVVVSSFF
                     ADIFKNNALNNGVLPVVVTPEFLNEIFQSVSENPNMTLTVDLENQTITNNATGKSEKF
                     DINVYKKECLIKGLDDIDYLLSKKDLIEAYEASRTF"
     gene            9173..10714
                     /locus_tag="PLM04_06040"
     CDS             9173..10714
                     /locus_tag="PLM04_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HON02670.1"
                     /translation="MVEIMDTTLRDGEQTSGVSFSAQEKLSIARMLLQEMCVDRIEIA
                     SARVSEGEFDTVKKICQWARNNGYIDKIEVLGFVDGKDSLNWIQSAGGKVVNLLSKGS
                     LKHCTYQLKKTPEQHVTDIKQIIAHATEMGISVNLYLEDWSNGMKDSEDYVYFLMDAL
                     KSENIKRFMLPDTLGVLNPEDTKNFCKKMIERYPNLHFDFHAHNDYDLAVANVFEAVK
                     VGVKGVHTTVNGLGERAGNAPLTSVLAVLHDHLKIKTNLREDKAYALSKMVETYSGIR
                     IPHNKPLIGENVFTQVAGVHADGDNKRNLYFNDLLPERFGRERQYALGKTSGKANILK
                     NLQQLGIDLDDNILRKVTERVIELGDKKQLTTMEDLPYIVSDVLNEQEEQSIKLLNYS
                     LSITYGLRPVATVKMEINGEVYEQTAAGDGQYNAVSKAMWKIYKILNKPTPELLDYEV
                     VIPPGGQTNAIVQTIITWKFNQVIFKTRGLDRDQTIAAIKATMKMLNLIEGGKLRSNK
                     YTDLP"
     gene            10824..11903
                     /gene="leuB"
                     /locus_tag="PLM04_06045"
     CDS             10824..11903
                     /gene="leuB"
                     /locus_tag="PLM04_06045"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HON02671.1"
                     /translation="MGKNINIAVLPGDGIGPEIIDQALKVTEAVCNKFQHDLTYEFGL
                     VGACAIDEVGEPYPKATHELCMQSDAVLFGAIGNPKYDNDPSAKVRPEQGLLKMRKEL
                     GLYANIRPVNTFPSLVEKSPLRAELVDGADFICIRELTGGIYFGRPQGRSEDGSTAYD
                     TCIYTREEIERIVHLAYRFAAQRRKKVTMVDKANILATSRLWRQITQEISAQYPDVET
                     EYMFVDNAAMRIIQSPKNFDVVVTENLFGDILTDEASVITGSLGMLPSASIGVHTSLF
                     EPIHGSYPQAAGKNIANPMAAILSAALMFEYAFVLKEEGALIREAVSACIEAGVVTED
                     IANGQKAYKTTEVGQWIVNYIDNKK"
     gene            complement(11937..12512)
                     /locus_tag="PLM04_06050"
     CDS             complement(11937..12512)
                     /locus_tag="PLM04_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HON02672.1"
                     /translation="MSCFQFLPESCHSPTKIENSDTYITNYLRSGDIILRKSYGLVSN
                     IIAAKLNDTIDVSHCGIIYIDANHTVYVIHSLSKAVSEKNGMQSCTLKKFMADSRIET
                     VQVVRFKKDSAQIIGKWAHYYLLKQIPFDNNYDLKDSSAFFCTELPIHIINKQFGINL
                     SSGNELPKFSIFLNPKYFDKVFFIKKANLNH"
     gene            complement(12527..12928)
                     /locus_tag="PLM04_06055"
     CDS             complement(12527..12928)
                     /locus_tag="PLM04_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HON02673.1"
                     /translation="MKNLNLFFLAAISLALGACSSNSNSPEAVAEKFNKAIYTADFNA
                     AKALCTDDSKQAVDLIASFVSEKAGDMKKANVKYETKSVNLSEDGNSADVEGVVLNAY
                     DLQNDKIADSTDTKVHLVKENGKWLVDYKLK"
     gene            complement(13166..15322)
                     /gene="rnr"
                     /locus_tag="PLM04_06060"
     CDS             complement(13166..15322)
                     /gene="rnr"
                     /locus_tag="PLM04_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HON02674.1"
                     /translation="MSKKTNRNTSTRIPRKQLINAISDIFNQHPRETYNYRQIAKMLN
                     IENQSQQQYIRQLLEELADEEFVTEISKGRFKANFPNGNSIIGTIDRQGVKTYVIPDD
                     GGEMIFIPERKTNHALLNDKVKVVLYARRKGQQQEGEVVEVLQRAQDTFVGTLQVSEN
                     FAFLVVDNRIMTSDIFIPRNKLNGGKNGQKAVVKLMEWQPDLKSPVGEVIDVLGDKGN
                     NNTEMHAILAEFGLPYRYPVEEEKAAEKIDDGITPEEIAKRRDMRKVTTFTIDPRNAK
                     DFDDALSIRKLNNGLWEIGVHIADVTHYVQPRTIIDREAEKRATSIYLVDRTIPMLPE
                     HLSNGICSLRPNEDKLTFSVVFQIDENADVKGYEIVHTVINSNRRFTYEEVQEIIESG
                     KGDYSAEILQLNKMAQILRKRRFENGAIAFERTEVQFEIDKKGRPISVFFKEQKESHQ
                     LIEEFMLLANRTVATHIGKPAKGSKAKTFVYRVHEVPSPDKLRDLSAFVKKFGYHFKT
                     SGKQTSVSKGINKLLDEVQGKKEQNLIETLAIRSMSKAVYSTNNVGHYGLAFDYYTHF
                     TSPIRRYPDMMVHRLLDRYLANGKSVNAEEWEEYCLHSSDMEQLAAKAERTSIKYKQV
                     EFMSERIGQIYDGIISGITEWGIYVEIIENKCEGMIPIRDLDDDYYIFDEKNYCLTGK
                     RYHKRYRLGDEIKIRVAKANLDKRELDFALAGNFGK"
BASE COUNT         4943 a         2844 c         3055 g         4797 t
ORIGIN      
        1 agctcaggcg ataaaggcgt tgcaagtaaa agaagcaggc gaacagaaac gagccttaac
       61 gcaggatttg atgggagggt gggggagtaa ttcccctacc tactcgatca tcttttcgga
      121 cataacttgt gaaagagtgt caaattgaag taaataatac aaatgtcact tttccaaaat
      181 catcagattt ttgctccaag ttgttgaaga aaaaattaaa cgtttttttt catgtcttcg
      241 gcaaattctt tcaagcgttt gtcaaggaca ggaatttgat ctggtcggac aaatgaaaca
      301 catatacgta aaccgtgatt gtccttacta cctgtaatgg agagtgaaat agcactgaga
      361 ccgtaataaa gaaaattctt catcaactgg ctgctcgtca tctccggata agccacagta
      421 aaataaaaac catctgccag ctcttcattt ttttctttgt aaacgatgta aaatccattt
      481 ttgagaaata tttccttcat tatttttgct ttttcggcat attctttcaa agcttccaga
      541 aaatttattt ttccatcatt cactgccttt aaagttgctg caaatgcata ttgagctgtg
      601 tgtgatgtac ctgccgtcgt agcatacagc gctccataag gaataaaatg tcccaaaacg
      661 tcgctggtaa agtagttttt aagttccgga taatgacggg tacgcaacga ctcggaaata
      721 atcatcatac caattcgctg tccggcataa ctgaaaattt ttgaagctga aagcaacaaa
      781 atgtagtttt cggtataacg agctacagtt ggctgataag gcggcacacc gggtctaccg
      841 taatcttttc tgaaatccat gccaaaataa gccaaatctt cgacaacaat aacgtcatat
      901 tcggttgcca attcgcctat aatttgcaat tcttcttctg taaaattgat ccatgaagga
      961 ttattgggcg aagaataagt aatggtgcaa atatctcctt cttttaaaaa tgaagttaat
     1021 gcagaacgaa gtctttcacc cctattttcc agaatgtcaa atgatttgaa tggaagcccc
     1081 atgagttgca gttgcgattt gtttaccgga aatccgggat caataaacaa cgtacagggt
     1141 ttatttttgt tggtgcgggc agcagccagc aaagcggcaa aacttccctg catggctcct
     1201 acggttggaa tgcagttcaa aggagtaaca tcaatgtcca tgaataattt tgcaaaacgt
     1261 gcggtttcgt ttttcacaaa aggaattcct tcaatatcgg gataaagagc agccaatccc
     1321 ctgtccaatg cttccttttc agcatctaca gttgtttgca aaggaggaag accgggcaca
     1381 cccatttcca tgtggataaa ttctacattg gttttttgtt caatttgagc aacaagtttc
     1441 cccatttcac gaatggagag tttatttata tcttcctgtc caaattgtct gaaaatctca
     1501 tccaccaaat ctttagaaat tgacatgttt tttaatttaa gaccattaaa tttttcgatt
     1561 atctatcact cttacagctt ttccttcact tctcggaata cttttcggct caacaagcgt
     1621 aacttttacg ctcaaaccaa gtgcactgtg aagcgaatgt tctatttttc tggaaagaga
     1681 catcaaatcc tttatggtat ctaccatttt agtttcgtcc agttccactt tcagttcaag
     1741 aatatcgaga ttgttttctc tatcaacata taacatgtaa tgtgcacttg tttcgcccat
     1801 ttcgagcagc acctgttcga tttgcgatgg gaaaagattt acacctctga taatcagcat
     1861 atcatcactt ctaccagtaa ttttcttcat tcttaccaat gtacgtccac attcacatct
     1921 ttctcgattc agtgtggtca aatcgtgtgt attgtagcgc aacaatggaa ttccttcctt
     1981 agtcaaggtg gtgaaaacca attccccctg ttggccgtcg ggtaaaggct ctttggtttt
     2041 gggatcgacc acttcgggga aaaagtgatc ttcatgaata tgtaaaccat catgaaactc
     2101 gcaatcattg gcaacgcccg gtcccataat ttcacttaat ccgtaaatgt caaaagcttt
     2161 aattccccac tttttttcca attcaagtct catattctca gaaaacggtt ccgctccaaa
     2221 tacacccgtt ttaagtttca tgtcggagag cgaataacca aattccgaca tagcatcagc
     2281 caaatgaagt gcatacgatg gagtacaggc taacacagtg gaaccaaaat cggacatgag
     2341 ttgtaattgc cgtcgtgtat tgcctgccga aatcgggaca accgtacaac ctacattttc
     2401 ggcaccgtaa tgaattccca atccgcccgt aaacagtccg taaccaaatg aaacctgaac
     2461 aatatcttca ggcgtaactc ccgacatgat cagacatcgg gcaagtactt cctgccagtt
     2521 ttcaatatct tttttggtat atccaacagt tgttggtttg cctgtagtac cgctggaagc
     2581 atgaatccgc acaatttcat tcatgggagc agcaaacaat ccaaatggat aattgtcgcg
     2641 caaatcggtt ttattggtaa aaggtaattt ggaaatatca tcaatagaac gaatatcgga
     2701 aggtctaaca cccttttcat ccattttttt tcgataaaat tccacatttt gataaacttt
     2761 gttgaccaat tctttcaatc tttcactttg cagctttcgc atttcactgc tactcatgca
     2821 ttcgatctct tcgttccaaa tcataattgt gcttttaaat cggattcgta tatgggtttt
     2881 aattgatatt tttgaattat ttcgatggct ttttcggcgt tgtcgggacg cattaccaat
     2941 atggatttgg agccaaaact gaaagcataa atatattcga tggcaattcc gttgtcagaa
     3001 aaatggttca atacccgcga taatgaaccc ggaacacctt ccagctcgag cgataaaact
     3061 tcatgaatgc gaactgtaaa attttcggtg cgaagggctt cggctgcttt ttcagcatcg
     3121 gaaaccagca ttctaagaag tccaaaatca atgctatcgg ccagcgtgag ggcaataatg
     3181 ttaatgttgt tgcgtgccag aaccgctaaa atctgattga gatgtcctgt tttgttttct
     3241 acaaaaacgg aaagttgacg gattttcatg gttgtcagta taaaatgaat atttatttaa
     3301 tttcgtaaat gaacggcgca aatttacaac aaattaataa atttgttggg cttgtgttgt
     3361 aattttttaa aaaggtttat acacgataaa agctgaattc gttcttgata ataaaaaaat
     3421 ctataaattt gtcgaaaatt tatacaccaa aaaattttat catgttccca tatccattac
     3481 ttcataggaa cgaattaatt cggcaaatga ataatttggg aaaatccaac actccatttt
     3541 ttttcctact ggattttatg gcgcaaaatg gatatattat caaaaatcaa gatctggacg
     3601 accaatatat acgatttcaa ttgtcggatg aaagtttttc caatcaagaa aaatcgacaa
     3661 aaaattcatt taattttcaa tggaatattc atcccgtaaa ttttgatgaa taccaattga
     3721 aatttcattt tgtaatggaa aatatccgat gcggaaattc ttttttactg aatttaacgc
     3781 agccaacaga agtaagtacc aacttaaatt tgcttgaatt gtatgagtta agtgtttcaa
     3841 aatacaaact atggctcaaa gatttattta ccgttttttc gcctgaaact tttataaaaa
     3901 ttgaggatgg aattatttcc acttttccca tgaaaggaac tattgatgct tctgttgcaa
     3961 atgccgaaga tgcgattttg aacgacttca aagaacaagc tgaacatgca actattgtag
     4021 atcttatgcg taatgattta agtatggttg ccgaaaaagt tgaagtcaag aaatacagat
     4081 acatcgataa aataattacc aatcaaaatg aactatttca aacgagttca gaaattacag
     4141 gagaattacc tgaaaattat tgttgttatt tgggaaatat tattatttcc atgcttcctg
     4201 ccggttcgat ttcgggtgct ccaaaacgta aaacattgga aatcatcaga gaaattgaag
     4261 gatatgacag aggtttttat accggaattt tcgggcaata tgatggaaaa aatttggatt
     4321 cagcggtact gatacgcttc attgagcaaa agaacggaca aatgattttc aaaagcggag
     4381 gaggaataac atcgaacagc gaagttttaa aagaatatcg agaaatgtta gaaaaagtgt
     4441 atgtaccgat ttattgaatc cataaaactg ttggatggaa ttttttatcg cctcaaattt
     4501 catcagaaga gaatgaatat ttgttcggaa ttattttatc cgaatgacga ttttattcat
     4561 ctccaatcgg ctttaaccga ttcacatcca ccacaaaagg gtttattcaa atgcaaaatt
     4621 acgtttgaat ctaaaatttt gaagatagaa tatttaccat acaaaaaaaa ggaaattaat
     4681 tctcttcgca tagtacatac cgagatagaa tcacgtccct ataaaaagga agatcgtaca
     4741 gattttaacg cagcatttga catgagagga aattatgatg acgttttgtt gatgaaaaac
     4801 ggcttattga cagatacttc tatttgcaat attgcccttt ttgacggttt ttcttggcaa
     4861 acgccaacca aaccattgat ttatggagtt aatagagcta atttgttaga agaaggaaag
     4921 attttcgaaa aagatattaa acttagcgat ttgcaaaatt attctgccat taggcttttt
     4981 aatgctttga tcgaatttgg cgaaattgaa ttgaatatca gcaatataca tttttaaaaa
     5041 agaaaaattg taaatttttt cagtgctaaa attgagaatt gaaacaaaac aatagaaaaa
     5101 aatttcactt tatattttgc atttagaaaa attgcagtat ctttgcgaag aatttacaaa
     5161 ttaaaacaaa acatgaaact gaattctcta catactttgc taagcagcat tattctactt
     5221 ttggcaaaag agcagaagtg agtttggtat gtagatatga taaccgattt tatttgaaaa
     5281 acctttctca cttaatgtaa gaaaggtttt ttactttatt atattaatga taaaattaac
     5341 caactttgag ttacaattca aaaaacaaaa aaatatggac agattattta tttttgacac
     5401 gactttacgt gacggcgaac aagttccagg ttgccaactc aatactgtcg aaaaaattga
     5461 agtagcaaaa gcgcttgaaa atttaggtgt cgatattata gaagcgggct ttcccgtttc
     5521 gagtcccggc gatttcaatt cggttgtaga aatttcaaaa actgttactt ggccggttat
     5581 ttgtgccctg acccgcgccg tagaaaaaga cattgaagtg gcagcagaag cacttcaata
     5641 tgccaagaag aaaagaattc acaccggcat aggaacttca gaatatcata tccgttacaa
     5701 gttcaattcc aatcaggaag aaattctgga acgctcgata gcagcagtaa aatatgcaaa
     5761 aaaatttgtt gaagacgtgg aattttattg tgaagatgcc ggtagaacag aaaatgagta
     5821 tcttgcacgc gtggtagaag ccgtaatcaa agccggagct actgttgtca atattcccga
     5881 caccacagga tattgcctcc cttgggaata tggtgaaaaa atcaaatatc taatggaaca
     5941 tgtggatggt attcacaaag ccataatttc tacacattgt cataacgatt tgggtatggc
     6001 aactgccaac accatatcgg gaatcatgaa tggagcacgt caagccgaag ttaccataaa
     6061 cggtatcgga gaacgtgcag gaaacacagc tttggaagaa attgccatga ttttgaaatg
     6121 tcataaaaaa ctgaatatcg aaacacaaat caataccaaa aagatttatt caaccagccg
     6181 tctggtttcc aatttaatga atatgcccgt tcagcccaat aaagcagtag ttggtagaaa
     6241 tgcttttgct cattcttcgg gaattcacca agatggcgta ttgaaaaaca gggagaatta
     6301 cgaaattatg aatccagaag acgtaggtat tgatcacaat tcaatagtgt tgacagcacg
     6361 gagcggacgt gccgccttga aacacagact gaatactttg ggcgtggaat tggcagataa
     6421 aaaactggat gctgtttatg aagaatttct gaagttggcc gacaagaaaa aagatataaa
     6481 cgacgatgat ttgctgatgc ttgccggtaa agaaaggtca gagaaagaac gtataaaact
     6541 cgactattta caggtaactt ccggtgtggg ggtaaaaccg gtagcaagta tcggactcat
     6601 tatcggcagt gaaaaatttg aagccgcttc agcaggaaat gggccggttg atgcagccat
     6661 caaggctttg aaaaaaatta tcaaccgaga aatgacattg caggaattta ccattcaggc
     6721 catcaataaa ggaagcgatg atgtgggtaa agttcacatg caggttgagc acgaaggtgt
     6781 ggtttattat ggatttggag ccaacaccga tattgttgct gcttccgttg aagcatatat
     6841 cgacgccatt aataaatttg ttgaataatt atgttcggca actaaacatt tttgtgtttt
     6901 caaatgttgc aaaagtaaaa ttattttacc gaaaaaataa ttaaaataca tatttaaaaa
     6961 atgattttta ttttattgtc aatcaaaaga aaacaaaatc ttttttcgtt acacaaatca
     7021 aaaaaacatg aatacattat ttgataaaat ttgggatgct catgtggttt ccacgattga
     7081 aaacgggcct actattttgt atatcgacag gcattatgtt cacgaagtta caagcccaca
     7141 ggcttttaat ggattgagag caagaggatt gaaagttttt cgtccgttac aaaccacttt
     7201 gactgccgac cacaatattc ctactctcca tcaggatcag ccggtgaaag accctgtttc
     7261 acgaaatcag ctggatacat ttgagaaaaa tgccagagac tttcagctac ccttgttttt
     7321 ccctatggga catgaaaaaa atggtgtggt tcatattatt ggtcctgaaa atggttttac
     7381 tcagcccgga atgaccatag tttgcggcga cagccatact tcaactcacg gcgcttttgg
     7441 tgccatcgct tttggaattg gaaccagcga agttgaaatg gtgttggcta cacaatgtat
     7501 tttgcaaaca cgtcccaaaa ccatgcgtat cacttttgac ggaaaattga ataaaggagt
     7561 aaccgcaaaa gatttggcac tttacatgat ttcaaaactt tcgacaggtg gagcgacagg
     7621 atattttgtc gaatatgccg gcgaagctat tcgcaatatg aatatggaag agcgaatgac
     7681 cgtttgcaac ctgagtattg aaatgggagc acgtggaggc atgattgcac ccgacgaaac
     7741 aacttttgcc tacttgaaaa accgcgaatt tgctcccaaa ggggaagaat ggaataaaaa
     7801 acttgcttac tggaaaactt tgtattctga ccaagatgct gttttcgaca aggaatatac
     7861 tttcgacgcc tccgaaattt ctccaatgat tacttatgga accaatcccg gtatgggcat
     7921 gggtattgat caaactattc caactttgga acaggtggac gaagccggga gaatttcttt
     7981 tgaaaaagca ttgaactata tgggatttcg tcccggtgaa aaattactcg gcaaaccaat
     8041 tgactacgta tttttaggaa gctgtaccaa tggacgaatt gaagacttcc gcacatttgc
     8101 acaatttgta aaagggaaaa agaaagccga caatgttgta gcttggctgg tacccggaag
     8161 cagaaaagtg gaaaaacaaa ttcacgatga gggcttgcat gaaattctaa tggaagcagg
     8221 ctttgagtta cgtcaaccgg gatgttctgc ctgtttggcg atgaatgacg acaaggttcc
     8281 ggccggaaaa tatgccgtat ctacttccaa ccgaaacttt gaaggacgtc agggaccggg
     8341 cgctcgaact atccttgccg gaccactggt ggccgctgct gctgccgtta cggggaaaat
     8401 taccgatccc agagaatttt tatcttaaaa ccaatttatt catttaataa aaaatcaaaa
     8461 aaaattgatt ggcatcagta taaatattac actgtaaatc aattaaaaaa gaaatattat
     8521 ggaaaagttt gttaaactga catcaactgt tgttccactt cctatcgaaa atgtggatac
     8581 agatcaaatt ataccggcaa gatttctaaa agcgaccaac aaggatggtt ttggagatca
     8641 tctttttgcc gattggcgtt acaataagga cggaagtccc aaagccgatt ttgttctcaa
     8701 caatcctacc tataaaggtt ctattttggt agcgggaaaa aatttcggtt caggttcaag
     8761 ccgcgaacat gcagcatggg ccatagccgg ttatggattt aaggtagtgg tttcaagttt
     8821 ttttgctgac attttcaaaa ataacgcatt aaacaacggc gtattgccgg tagttgttac
     8881 accagaattt ttaaacgaaa tatttcaatc tgtcagtgaa aatcccaata tgaccttaac
     8941 ggttgatctc gaaaatcaga ctattaccaa caatgcaacc ggcaaatccg aaaaattcga
     9001 tatcaatgtg tacaaaaagg aatgtctgat aaaaggcctt gacgatatag actatttgct
     9061 tagcaaaaag gatttgattg aagcatatga agcttccaga actttttaag ttttaaattt
     9121 taattttttt attcaatttt tctcctttag gggaaatttc aaagcaagct ttatggtaga
     9181 aataatggat acaaccctgc gtgatggcga acaaacttca ggagtttcgt tctcagctca
     9241 ggaaaaattg agtattgcca gaatgctgtt gcaggaaatg tgtgtagata ggattgaaat
     9301 tgcttctgca cgcgtttcgg aaggtgaatt tgacactgtc aaaaaaatat gtcaatgggc
     9361 acgaaataat ggttatatcg acaagattga agttttgggg tttgttgatg gaaaagattc
     9421 actcaactgg attcaatcgg ccggcggaaa agttgtcaat ctcctttcaa aaggttcgtt
     9481 aaagcattgc acttatcagt tgaaaaaaac gcccgagcaa catgttaccg atattaaaca
     9541 gataattgcc catgcaacgg aaatgggaat ttctgtcaat ctttatttgg aagactggtc
     9601 gaatgggatg aaagattcgg aagattatgt atattttctg atggatgctc tgaaatccga
     9661 aaacattaaa cgtttcatgt tacctgatac gttgggagtg cttaatcctg aggatacaaa
     9721 aaacttttgc aaaaaaatga ttgagcgtta tcccaattta catttcgact ttcatgcaca
     9781 caatgattac gatttggctg ttgcaaatgt ttttgaagca gtaaaagtcg gtgtaaaagg
     9841 cgttcatacc actgtcaacg gtttgggaga aagagccgga aatgctccat tgacaagtgt
     9901 tttggctgta cttcacgatc atttgaaaat aaagaccaat ttacgtgaag acaaagctta
     9961 tgcgttgagt aaaatggttg aaacctattc cggtattcgt attccgcaca acaagccact
    10021 tatcggcgaa aatgtattca cgcaggttgc cggcgttcat gccgatggag acaacaagag
    10081 aaatctctac tttaacgatt tgcttccgga aagatttggt cgtgaaagac agtatgcttt
    10141 ggggaaaaca tccggcaagg caaatatttt gaaaaatctt cagcaattgg gcattgacct
    10201 tgatgacaac attttgcgaa aagttacaga acgtgttatt gagttggggg acaaaaaaca
    10261 gctcaccact atggaagatc ttccttacat tgtatctgat gtactcaacg aacaggaaga
    10321 acaatccatt aaactgctca attattcgct atccataact tatggattac gccctgttgc
    10381 gacggtaaaa atggaaataa acggagaagt atatgaacag acggctgccg gcgatggaca
    10441 atacaatgct gtttccaaag ccatgtggaa aatttataaa attctgaata aaccaacacc
    10501 cgaattgctt gattatgaag tggtaattcc acctggtggt caaacaaacg ctatcgttca
    10561 aacaattatt acatggaaat tcaatcaagt tattttcaaa accagaggat tggatcgaga
    10621 ccagaccatt gcagccatca aggctaccat gaaaatgctg aatctgatcg aaggcggtaa
    10681 attacgttca aataaatata cagatttacc ttaattcttt attttgaaaa atattatact
    10741 gcatcgtgcg aaagttcaga aattatgctc atttttgtaa agagtaagaa aaaaagaaaa
    10801 tcactttaaa aacaataaat aaaatgggaa aaaatattaa tatcgccgtt ttgccgggtg
    10861 atggtattgg tccggaaatt atcgatcagg cattgaaagt aacagaagct gtttgtaaca
    10921 aatttcaaca tgacctcact tacgaatttg gactagttgg agcttgtgcc attgatgaag
    10981 ttggagaacc ataccccaaa gcaacacatg agttgtgtat gcaatccgat gctgtacttt
    11041 ttggagccat aggaaatccg aaatacgata atgatccttc tgctaaagtg cgtcccgagc
    11101 agggtttgct taaaatgaga aaagaactgg gattgtatgc caacattcgg ccggtaaata
    11161 ctttcccttc actggttgaa aaatctccat tgcgagcaga attggtggac ggagccgatt
    11221 ttatttgcat ccgcgaatta acgggaggaa tctattttgg ccgtcctcaa ggaagaagcg
    11281 aagatggaag tacggcttat gatacctgta tttacacacg ggaagaaatt gaaagaattg
    11341 ttcatttggc ttatcgcttt gctgcacaac gtcgcaaaaa agtaactatg gttgataaag
    11401 ccaatatttt ggctacttct cgactttgga gacaaataac tcaggaaatt tcggctcaat
    11461 atccggatgt ggaaacagaa tacatgtttg tcgacaatgc ggcaatgcgg attatacagt
    11521 cgccaaaaaa tttcgatgta gtggtaacgg aaaatctctt tggcgatatt cttaccgacg
    11581 aagcttcagt gattaccggt tcgttgggaa tgttgccatc tgcttccatt ggtgttcata
    11641 cttcactttt tgagccaatt cacggttcct atccgcaagc agcgggtaaa aatatagcca
    11701 atccaatggc agccatcctt tcggcggctt taatgtttga atatgctttt gtgttgaaag
    11761 aggaaggtgc tttgattcgc gaagccgttt cggcatgtat cgaagccggc gtggtaaccg
    11821 aagatattgc caacggtcaa aaagcataca aaactactga ggttggacaa tggatagtta
    11881 attacatcga taataaaaaa taaaataaga tttataatct ttgcttcagg cggactttaa
    11941 tgattaaggt tcgctttttt tataaaaaag actttatcga aatacttggg attcagaaat
    12001 atagagaatt ttggaagctc attgcccgaa gaaagattga tcccgaattg tttgtttatg
    12061 atgtgaatgg gaagttctgt acagaaaaat gcagaagaat cttttaaatc gtaattattg
    12121 tcaaaaggga tttgttttaa aagataatag tgagcccatt tgccaataat ttgagcggaa
    12181 tcttttttga acctgacaac ctgtaccgtt tcaatacgac tgtcggccat aaatttttta
    12241 agcgtgcaac tttgcatgcc atttttttcc gaaactgctt ttgaaaggga atgaataacg
    12301 tacacggtat gattggcatc aatataaata atgccacaat gcgaaacgtc aatcgtatcg
    12361 ttcaatttag ccgctattat gttgctgaca agcccgtaac tttttcgtaa aattatgtcg
    12421 ccgctacgaa gataattggt aatataagta tctgaatttt caatttttgt cggagaatgg
    12481 caactctccg gcaaaaattg aaaacacgac aacaaaagaa agcaaattat ttgagcttgt
    12541 aatctaccag ccacttgccg ttttctttaa ccaaatgaac ttttgtatcg gttgaatcgg
    12601 ctattttatc gttctgcaaa tcgtaagcgt ttaacacaac accctcaaca tctgccgaat
    12661 ttccatcttc ggataaattg accgattttg tttcgtattt aacatttgct tttttcatat
    12721 cacctgcttt ttcggaaaca aaagaagcaa ttaaatctac tgcctgcttg ctgtcgtcgg
    12781 tacataaggc tttggctgca ttgaaatcgg ccgtataaat agccttgtta aatttttctg
    12841 ctactgcttc aggagaatta ctattggaag aacaagctcc aagtgccaga gaaatggctg
    12901 ccaaaaaaaa taaattcaaa tttttcataa tcttttaaat tttagttgaa ttaattaccg
    12961 aattttaatt gtcggctgtt taaaggcgta aaagtataaa ataaatttac gaaaaacaaa
    13021 caaaaactaa aattaattca aaataattat aaaaaatgag aacttctaaa aataggaatt
    13081 acaagtataa atatcgacat aattaaaagt ttgtcggata aacttgatta aaaatctttt
    13141 tctcctgaat gatttttctt ttgtgtcatt ttccaaagtt accagccaat gcaaaatcga
    13201 gctctctttt gtcgagattg gctttggcaa ctcgaatttt aatttcgtct cccaagcggt
    13261 agcgtttatg gtagcgtttt ccggtaagac agtagttttt ttcgtcaaaa atgtaataat
    13321 cgtcgtccaa atcgcgaata ggaatcattc cttcacattt gttttcaatt atttctacgt
    13381 aaattcccca ttcggttatg ccggatatga tcccatcgta gatttgtccg attctttcag
    13441 acataaattc cacctgtttg tatttgatgg aagtgcgttc ggctttggct gccaattgct
    13501 ccatgtctga gctgtggagg caatattctt cccattcttc tgcattgaca cttttcccgt
    13561 tggccaaata tctgtcgagc agacgatgca ccatcatgtc gggataacgg cgaatgggag
    13621 aagtgaaatg ggtatagtaa tcgaaagcaa ggccataatg accgacattg ttggttgaat
    13681 aaactgcttt ggacatggag cggatagcca gcgtttctat cagattttgt tcttttttcc
    13741 cctgaacttc atccaacaat ttgttgattc cctttgaaac tgaggtttgt ttaccggaag
    13801 ttttgaaatg atagccaaat tttttaacaa aagcagacaa atcgcgtaat ttatcagggc
    13861 ttggaacttc atggacacga tagacaaagg ttttagcttt actacctttt gccggcttgc
    13921 caatgtgtgt ggctacagta cggttggcca gcagcataaa ttcttcgatc aattgatggg
    13981 attccttttg ctctttaaaa aaaaccgaaa tgggtcgtcc ttttttgtcg atttcaaatt
    14041 gaacttcggt acgttcaaaa gcaatggctc cgttctcgaa acgtctttta cgaagaattt
    14101 gtgccatttt gttcaactga agaatttctg ctgaataatc accttttcca ctttcgatga
    14161 tttcctgaac ttcctcgtag gtaaatcgac gattactatt gattacggta tgaacaattt
    14221 cgtagccttt tacatctgca ttttcatcaa tttgaaaaac aacggaaaaa gtcagtttgt
    14281 cttcattggg acgaagcgaa caaattccgt tagacaagtg ttcgggtaac ataggtatcg
    14341 ttctgtctac caaatagata gaagtggctc ttttttctgc ttcacggtca ataatggttc
    14401 tgggctgaac gtaatgagtt acatcggcga tgtgaacgcc aatttcccaa agtccattat
    14461 tgagttttct gatggaaagt gcatcatcaa agtctttggc attgcgcgga tcgatggtaa
    14521 aagtggttac ctttctcatg tcgcgtcttt ttgctatttc ttcgggtgta attccgtcgt
    14581 caattttttc ggctgctttt tcttcttcaa ccggatatct gtacggcaac ccaaattcgg
    14641 ccagaatggc gtgcatttcg gtattattgt tgcctttatc tcccaagaca tctattactt
    14701 cgcctacggg acttttcaga tcgggttgcc attccatcag ttttaccaca gctttttgac
    14761 cgttttttcc accgttgagt ttgtttctgg ggataaaaat atcactggtc atgatgcggt
    14821 tgtccaccac cagaaaggca aaattttcag aaacctgtaa agtaccgaca aacgtatcct
    14881 gtgcgcgttg caaaacttcc accacttctc cttcctgctg ttggcctttt cgccgagcat
    14941 acagaacaac ttttacctta tcgttaagca gggcatggtt ggttttgcgt tcgggtataa
    15001 aaatcatttc tccgccatcg tcgggaatca cataagtttt aacgccttgt cggtcaatgg
    15061 ttccgattat gctgtttcca ttagggaaat tggctttgaa acgtcctttc gaaatttccg
    15121 taacaaattc ttcatctgcc aattcctcga gcagttgtcg gatgtattgt tgctgcgatt
    15181 gattttcaat attcagcatt ttggctattt gtcggtaatt gtatgtttcg cgcggatgct
    15241 ggttgaaaat atcgctgatg gcgtttatca attgtttacg cggaattcgc gtgctggtat
    15301 ttctatttgt ttttttcgac atgtttattt gttttttgaa tggaagattt taaaaatttt
    15361 ccattatttt tttgagttaa tgtttgatta tcaataaatt tttgctgtat taaaaagtct
    15421 ccggatagta tttttgcggt caggagaaat aaaaatgcaa agttacgact ttttcttcaa
    15481 aaaattttga tggttacgtt caacttagcg tccgaattga gcagaaagta ttaaattttg
    15541 gtttttgaat atttcaattt tattacattg tttataactt ttcgttgctg aattcaagtc
    15601 acaaatgcaa ctttttggtt atacaaattt cgtaataaa
//
LOCUS       DAOTAX010000048        15371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3224_length_15371_cov_31.887960, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000048 DAOTAX010000000
VERSION     DAOTAX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_15371_cov_31.887960"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..621)
                     /locus_tag="PLM04_06065"
     CDS             complement(448..621)
                     /locus_tag="PLM04_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02675.1"
                     /translation="MKDFLPYLGSIIILIGVALLAYYQFGGHPSNLILGTAGILMIVG
                     FFTHLFLNKKIVE"
     gene            868..2823
                     /gene="dnaG"
                     /locus_tag="PLM04_06070"
     CDS             868..2823
                     /gene="dnaG"
                     /locus_tag="PLM04_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HON02676.1"
                     /translation="MIDQATIDRIMEAAQIQDVVSDYVTLKKRGVNMIGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCGKGGNAVHFIMEHEQLSYYDALKFLARKYNIEVQERELTDE
                     EKELHNERESLLLVNEFAQKYFSDTLYHHIDGKTIGMSYFQERGIREDMIRKFQLGYC
                     LDQQDAFTQKALKAGYNKKYLVKTGLTIETENNRLTDRFRGRLIFPVHSLSGKIIAFG
                     GRVIKKDDKTAKYLNSPESEIYHKGNELYGMYFAKQAIVKEDRCLLVEGYTDVISLHQ
                     SGIENVVASSGTSLTPAQIRLIHRFTNNVTVLYDGDAAGIKASLRGIDLLLEEGLNIK
                     VVLLPEGEDPDSFSHKLNAVDLLNFIRDNSVDFIRFKTKLLMEDAANDPIKRSNLILD
                     IVRSIAIIPEPIVRAEYVKECSNLLQVNEEILYHEINRLKRKESEKKLGQSNVSASST
                     EPKPQPVSPQEMLNAENQRFEQEERNILQVLIKYGKSALYYSDEENRSPITVEDFIIE
                     DLEQDQLEFVHPLYAQMLQEYKEQCLKKNINVQNFFLHHIHPEISKLTAELISEKYTL
                     SKIYKKTRMDEPEIDKVREFAERAVLEYKNSIVLSQIKQKLLEMKIAAENHDDELVRI
                     LMIQISQLEEIKNQLAKNLGERIVIKL"
     gene            2836..3483
                     /locus_tag="PLM04_06075"
     CDS             2836..3483
                     /locus_tag="PLM04_06075"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HON02677.1"
                     /translation="MTYEELIHDTEYKELASLSHNEIKNFIVNEITENQQWARAANLY
                     QLTGILAFMLGTFKAFMPYFVHRQAFGLLWLLAGIVFTFTILIVFHELIHALAYWAMG
                     ARHLFFGCQPKKFMFYVQADKAVVGYRQFKIIALAPTVLISFLSLLGMIIFYHQPAFY
                     FFISIFGLHSLFAAGDFGLLCFFQNRPELEIVTFDVKEEGKTYFYGKEKRKNIAV"
     gene            3548..4318
                     /locus_tag="PLM04_06080"
     CDS             3548..4318
                     /locus_tag="PLM04_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON02678.1"
                     /translation="MILFDQIVFGPVHSRRLGTSLGINLLPVDAKICSFDCLYCECGF
                     NTSGKHAKLPTRMEVHNALEMRLKEMASDGQIPDIITFAGNGEPTLHPDFAGIIDDTL
                     ELRNKYCPNAKVGVLSNATQIHKPDVFNALKKVDDNILKFDSAFDHTLQLLDRPVRKY
                     ITVRWLIEHLKKFEGKMIVQTLFIRGEVNGEKIDNTSDKEIEAWLKALKEMNATQLMI
                     YTINRETPLKTLEKIPLEELNRIAEKAKSYGFDVSVSG"
     gene            4334..5278
                     /locus_tag="PLM04_06085"
     CDS             4334..5278
                     /locus_tag="PLM04_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02679.1"
                     /translation="MKILVCPLNWGLGHAARCVPIIRQLITEGNDVVIAADGFPLKFL
                     QLEFPELRFLKAPTQNVRYSTGNSQIWAMFKSIPRIFGNIFQDHCWLHRLLKQEHFDR
                     IISDNRFGCWSKRTYCIYITHQLMVKMPPELRLLEPIVWRLHRFFIHRYDECWIPDLA
                     GDENFSGDLAHRYPLPENAHFIGILSRFQQLKNIIPNYTYETVMVLSGPEPQRTIFEK
                     QLTEKFSKDTVKSLMIQGLPQEKQTSVQVGNLTKVSHLSTQEMASLLAGAKKIIARSG
                     YSTIMDLQALNCLDKAILIPTPGQTEQIYLAELHKKRR"
     gene            complement(5473..5871)
                     /locus_tag="PLM04_06090"
     CDS             complement(5473..5871)
                     /locus_tag="PLM04_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HON02680.1"
                     /translation="MEEFFLEKKSKFSFNKTITEFTNIVQEGGWKVVYVHDLQETMAR
                     NNKEVLPVKVLELCSPSHAYSILGKDEWRTFSSMMPCRISIYEKSDGNTYVSQMNISM
                     FAPLMEKEIQGIMNISYAEVQNFVSRIIEN"
     gene            complement(5887..6381)
                     /gene="trxA"
                     /locus_tag="PLM04_06095"
     CDS             complement(5887..6381)
                     /gene="trxA"
                     /locus_tag="PLM04_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HON02681.1"
                     /translation="MKKLTGIFLMITFIALSACAGSNGENKQNNSQNKNEEKLMGTIH
                     LTKEEFLKKVVNYEASPNEWKYLGDKPCVIDFYADWCGPCKAVAPILEELAKEYDGQI
                     YIYKIDTDKEQELAAAFGIRSIPTLLFCPMNENPQMAQGALPKDVLKNAIDEVLLKKE
                     KVKQ"
     gene            complement(6694..6921)
                     /locus_tag="PLM04_06100"
     CDS             complement(6694..6921)
                     /locus_tag="PLM04_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HON02682.1"
                     /translation="MLKKFLSRHWLKVVGVLLGSIGGFAYFYFVGCKSGTCPISSNPY
                     ISIVYGAVLGYLFFDMFKVKSKNTNANDQED"
     gene            complement(6935..7240)
                     /locus_tag="PLM04_06105"
     CDS             complement(6935..7240)
                     /locus_tag="PLM04_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HON02683.1"
                     /translation="MEQINNINIDQMEEAAYKLRAISHATRLCIISLLSEKEELNVSQ
                     ISEELNCEQSLLSHHLTDMRAKGILNCRRDGKNCYYSLKNKEIVQILDCIRSCQCTN"
     gene            7422..8474
                     /gene="aroB"
                     /locus_tag="PLM04_06110"
     CDS             7422..8474
                     /gene="aroB"
                     /locus_tag="PLM04_06110"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HON02684.1"
                     /translation="MTSSSTIICENFIAELNEFIEYQKPDGIFVLVDENTEKFCLPLL
                     LQSEKLKNCQVITIPSGDENKNIHTAIEVWNYLSSHGATRRSLMINAGGGMVSDLGGF
                     AAATFKRGISYINVSTTLLGAVDAATGGKTGINFMGLKNEIGVFAPADKVLINVDFFR
                     TLDDKNLRSGFAEMIKHSLLDSREHWKNILKFNLDTIDYELLKHRVAESVKIKEQIVE
                     KDPKEQNLRKALNLGHTFGHAFESFSYSQHQPVLHGYAVMYGLVCELYLSHVKLNFPK
                     EDLLKLVTLAKENYGKISFCCDDYEQLYELMTHDKKNVSDTINFTLLSEVGKIELNQT
                     ATKKEIFECLDFYQEM"
     gene            8534..8893
                     /locus_tag="PLM04_06115"
     CDS             8534..8893
                     /locus_tag="PLM04_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HON02685.1"
                     /translation="MKKTLVIGASTNPERYAYKAAQRLLENGHEIELLGLRPGEIFGK
                     SIDTEKKQYPDIDTVTLYVGPKNQPEYYDYILSLHPKRVVFNPGTENPEFEALLEANG
                     IQAQEACTLVLLSTQQY"
     gene            8957..9673
                     /gene="dapB"
                     /locus_tag="PLM04_06120"
     CDS             8957..9673
                     /gene="dapB"
                     /locus_tag="PLM04_06120"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HON02686.1"
                     /translation="MKIALIGYGKMGKAIERIAKERGHEIVCIIDIDNLNDFESDKFK
                     SADVAIEFTHPGVAMSNYRKTFAAGIPLVSGTTGWTNELPALKKEVEEKGYTFFWSSN
                     FSLGVNIFMAVNKYLASLMNRFSDYDVEITEIHHIHKLDAPSGTAITLAEDMLENLER
                     KTCWVKEEAKQPHELAIKSIREDEVPGIHIIRYDASFDAIEFKHEAKTRDGFALGAVM
                     AAEFAVGKKGWLTMQDLLKF"
     gene            9774..11174
                     /locus_tag="PLM04_06125"
     CDS             9774..11174
                     /locus_tag="PLM04_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HON02687.1"
                     /translation="MTSEPHTSRFHQPRRQWIKAAIWSLIYILFIAWVGNFWWLLLLP
                     VIFDMFITKFIPWTWWKKTKNKTLRSIYSWIDAIVFALVAVYFINIYLFQNYQIPTSS
                     LEKTLLVGDFLFVSKASYGPRIPNTPLSFPLVQNTFPIINTKSYLEKPQWEYKRLKGF
                     DTIAHNDIVVFNFPTGDTVCLKQQNPDYYQLCYYYGRDVVRSRKDIFGEIVYRPVDRR
                     ENYVKRCIGLPGDWLQIKSNQVYINGEKQKPFPGVQFNYLVQTDGTRLSDELLTKLGI
                     SKDDRLILNTSQNPQEIMALGLDPSFPIYHFPLTGEMLQKLQRVTGVVKIVIEPEMYG
                     GDVFPLGGGKNWTRDNYGPIYIPKKGAVLELNAYNYPIYERVIRTYENNKLEYKNGIF
                     YINDRPEKTYRFKMDYYWMMGDNRHNSADSRYWGFVPEDHIVGRPVVVWLSLDKDKSL
                     FGGKIRWNRFFKDARR"
     gene            11266..11874
                     /locus_tag="PLM04_06130"
     CDS             11266..11874
                     /locus_tag="PLM04_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HON02688.1"
                     /translation="MLSMSYLAPIDYFMTLTNARSAVIEQHEYYQKQTYRNRCRIAGA
                     NGVLDLIIPVEKTANNKATVRDIRISGHNDWQRQHWKAISSAYLSSPFFEYYLDDFRP
                     FYEKNWLFLWDFNMEIQNKIFELLEIYPKLEFTDKFIDKIEENILDLRLQFHPKKQPF
                     LKIPSYYQVFENKFGFISGLSIIDLLFNMGNESLLWLLGKAE"
     gene            11939..12955
                     /locus_tag="PLM04_06135"
     CDS             11939..12955
                     /locus_tag="PLM04_06135"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HON02689.1"
                     /translation="MELDWNNIGFGYVKTDYNIRCSYQNGEWGEFEVHEDEYLTLHMA
                     ATCLHYGQEVFEGLKAFRGIDGKIRVFRMDENAKRMQSSCKGIVMQPFPVDRFQEAVL
                     KVVKLNERFVPPYESGASLYIRPLMIGTSAQVGVKPSREYLFILFVTPVGPYFKGGFQ
                     TTPYVILREFDRAAPLGTGIYKVGGNYAAGLAADEKAHSLGYSAVFYLDAKEKKYIDE
                     CGAANFFGIKNNTYVTPKSTSVLPSITNKSLMTLARDMGMKVEQRPVPIEELSTFEEA
                     GACGTAAVISPIQRIDDLDENKSYVFSPDGKPGVICEKLYKKIRAIQYGEEPDIYGWT
                     TIVE"
     gene            13201..15309
                     /locus_tag="PLM04_06140"
     CDS             13201..15309
                     /locus_tag="PLM04_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HON02690.1"
                     /translation="MSIFFLFTGAKADEGMWMLPYIEQLNIQKMNGMGCSLSAEDIYS
                     DKNISLKDAVIVFGNGCTGVVVSPQGLIFTNHHCGFDAIQQHSSVEHNYLKDGFNAVR
                     LEDEISTPGLSVKFLVKIEDVTNQVLSHLPDSLSEKSRNGKIYEISDSICKAAEKDTH
                     YLAEVKPFFAGNEFYLLVYEEFKDVRLAFAPPSSIGNFGGDTDNWMWPRHTGDFSVFR
                     VYATPDGKPAEYSKDNVPYSPKRFASVSNKGYQTNDFTMILGNPGSTSRYLTSFGVKF
                     RMETGNQARIDVRGIKQTIWRSFMRKDEAINIAYANKYASSSNYWKNSIGMNKAIQKL
                     NVLERKREQEAAFLEWTKSNPEREKKYKDVLKTLEDGYSTLYPYARALNYLRESLMSG
                     VELPRIAQKASQLISQNLPKDSLIKEIEEIYKDYRPEVDQAVFAAMLDVYKNSVDADA
                     LPAFYQEIQKKFKGNTTLYAASIFQNSAFSSADKLKKNLQKKKIKIANDPALIFGNEV
                     RNTYEQISSGIYAQTSEKVKEAERIYFSGLREMYEEQGKKLYPDANFTMRMTYGKIGG
                     YQPADAVDYHYYTTTKGILEKENPTDPEFIVPDKLKKSILEKDFGKYLDPQTKDMHVN
                     FISNNDITGGNSGSPVFNGKGELIGLAFDGNWEAMSGDIVFEPELQRTISVDIRYVLY
                     VMDKIGGAQRLIDELSISGY"
BASE COUNT         4986 a         2873 c         2979 g         4533 t
ORIGIN      
        1 ttaatgatat taaagatgtg attctttttt gccgcttgaa acgaataaaa taaaccctat
       61 cctgataatt tgaaaacaca atcgggatag aaacatttct tctgtcaaaa aaactcattg
      121 agaaacctcc atgtattgca tacacaaaca cgaatgaata taatattcag acatgtggct
      181 ccatcagacc attaaaaaat aaattatatt ctgatgaaaa gtgtaaacgt aagctatcct
      241 ttagaaatat ttataaaaaa atccaaacca tttttgaagt tcggattctt tttttgaaaa
      301 cggaatcagt ttgacagttc tatttattca gcatatcatt cgctgatttt tctaccaaca
      361 aacaataaag accactttgc cccatcaatc aagattacta ccgactgaaa ttccaaaggt
      421 aaatggtaat ttttctgttg cagtaggtta ctcgacaatt tttttattca ggaaaaggtg
      481 agtaaaaaaa ccaacaatca tgagaatacc tgcagttcct aaaataagat tagaaggatg
      541 tcctccgaat tgataatatg caagcaatgc aacacctatc aaaatgatta tagaaccaag
      601 ataaggcaaa aaatctttca ttgtaatgat attttttatg ttgaaacttc aaactgcaaa
      661 ttaaataatt ttttgtcagc taatcaaatt atttttttaa aaattgaaaa actcactaca
      721 aaaaattggc acaaccctcc atcattaagg aaaaaaccgc agatctaaaa tttttaaatt
      781 tttctcggct aaattcttga ataaaatcag tagatttgta tttgatttca aagagactga
      841 tttttttaca aaattcttta tattagcatg atagaccaag ccaccattga cagaattatg
      901 gaagctgcac aaattcagga tgtagtttcc gattatgtta cgctcaaaaa aagaggcgta
      961 aacatgattg gtttgtgtcc gtttcacaac gaaaaaacgc catcgtttac cgtttcacct
     1021 gctaaaggaa ttttcaagtg ttttggttgc ggaaaaggtg gaaacgctgt tcacttcata
     1081 atggagcatg agcaactgtc gtattacgat gcattgaaat tccttgccag aaaatacaac
     1141 attgaagtgc aggaacgtga gctgaccgat gaagagaaag aacttcacaa cgaacgggaa
     1201 agtttgctgc tggtaaacga atttgcccag aaatattttt ccgatacgct ttatcatcac
     1261 atcgacggga aaaccatcgg tatgagttat tttcaggaac gcggtatccg agaagacatg
     1321 attcgtaaat ttcagttggg ttattgcctc gatcagcagg atgcttttac gcaaaaagct
     1381 ttaaaagccg gttacaataa aaaatatctt gtaaaaaccg gacttaccat cgaaacggaa
     1441 aacaatcgcc ttaccgaccg ttttcgcgga cgactgatat ttccagttca ttccctttct
     1501 ggcaaaatta ttgctttcgg cggaagggta ataaaaaaag acgacaaaac ggctaaatac
     1561 ctcaattcgc ccgaatcgga aatataccac aagggaaatg agctctacgg aatgtatttt
     1621 gccaaacagg ccatcgtaaa ggaagaccgc tgtttgctgg tggaaggtta taccgatgta
     1681 atttctcttc atcaaagcgg cattgaaaat gtcgtagctt cttcgggcac gtcgcttacg
     1741 ccggctcaaa ttcgtcttat tcaccggttt accaacaatg ttaccgtgct ctacgacggc
     1801 gatgctgcgg gcattaaagc ttcgttgaga ggaatcgatt tgctgctgga agaaggcttg
     1861 aacataaagg ttgttttgtt gcccgaagga gaagatcccg attctttttc ccacaaactc
     1921 aatgcagtgg atctcttgaa tttcattcgg gacaattcgg tggattttat ccgcttcaaa
     1981 accaaacttt tgatggagga cgcagcaaat gaccccatca aacgttccaa cctgattctg
     2041 gacatcgtgc gaagtattgc cattattccc gaaccgattg tgagagccga atatgtgaag
     2101 gaatgcagca atctgcttca agtgaacgaa gaaattcttt accatgaaat aaatcggctt
     2161 aaaagaaaag aaagcgaaaa aaaacttgga caaagcaatg tttcggcttc ttctaccgaa
     2221 ccaaaaccac aacccgtttc tcctcaggaa atgctgaatg ccgaaaacca gaggtttgag
     2281 caggaagaaa gaaatatact ccaagtattg attaaatatg gtaaatcggc attgtactat
     2341 tctgacgaag aaaaccgttc accgattact gtagaagatt ttattattga agacctcgaa
     2401 caggatcagc ttgaatttgt tcatccgctc tatgctcaaa tgcttcagga atataaggaa
     2461 caatgcttga aaaaaaatat taacgttcaa aattttttcc tgcatcatat tcatccggaa
     2521 atcagcaaat tgacggcaga actgatttca gaaaaataca ccctcagcaa aatttacaaa
     2581 aaaacgagaa tggacgaacc cgagatcgat aaagtgaggg aatttgccga acgtgccgtg
     2641 ctggaataca aaaacagcat tgttctctcg cagattaagc aaaaattgct cgaaatgaaa
     2701 atagcggcag aaaaccacga cgatgagttg gttcgtattc tgatgattca aatcagtcag
     2761 ttggaagaaa ttaaaaatca acttgcaaaa aatcttggag aacgcattgt cattaaatta
     2821 taaacagctt cataaatgac atacgaagaa ttgattcatg acaccgagta caaagaactc
     2881 gcatctcttt cacataacga aataaaaaat ttcattgtca acgaaattac cgaaaaccaa
     2941 caatgggcac gtgctgcaaa tttgtaccaa ttgacaggaa ttctggcttt catgctggga
     3001 actttcaaag ccttcatgcc ttattttgtc catcgtcaag ccttcggttt gctttggctg
     3061 ctggcaggca ttgttttcac ctttaccatt ctcatcgttt ttcatgaatt gattcatgcg
     3121 ctggcctatt gggcaatggg ggctcgtcat ttgttttttg gctgccagcc caaaaaattc
     3181 atgttttatg ttcaagccga caaggctgtg gtgggttacc gtcagtttaa aataattgct
     3241 ttggcaccaa ccgttttaat cagtttcctt tcgctgcttg gaatgattat cttttatcat
     3301 caaccggcat tttatttttt tatttcgata tttggtttac acagcctctt tgctgcagga
     3361 gattttgggc tgttgtgttt ttttcaaaac cgtcccgaat tggaaattgt tactttcgat
     3421 gtgaaggaag aaggtaaaac ctacttttat ggaaaagaaa aaagaaaaaa tattgctgtg
     3481 taggctttga aaggaaagca attttttcgc aaaataattt aacgttattt gaaaatatcg
     3541 tagaagaatg attttatttg atcagattgt ttttgggcct gttcatagca ggcgtcttgg
     3601 cacttctctg ggaatcaatt tattgcctgt agatgccaaa atttgcagct tcgactgtct
     3661 gtactgcgaa tgcggattta atacttccgg aaagcacgcc aaattgccta caaggatgga
     3721 agtacacaat gcgctggaaa tgaggttgaa agaaatggca agcgacggac aaatacccga
     3781 tattataact tttgccggaa acggagagcc gacacttcat ccggattttg ccggaattat
     3841 tgatgataca cttgaactga gaaataaata ttgtcccaat gccaaagttg gagtgttgtc
     3901 gaacgccaca caaattcaca aaccagacgt tttcaatgcc ttaaaaaaag tggatgacaa
     3961 cattctgaaa ttcgacagtg ctttcgatca tactttacaa ctgctcgacc gtccggtaag
     4021 aaaatacatc actgttcgct ggctgattga acatttgaaa aagtttgaag gtaaaatgat
     4081 tgtgcaaact ttatttattc gtggcgaagt gaacggagaa aaaattgaca atacttcgga
     4141 taaggaaata gaagcatggc tgaaagcatt aaaggaaatg aatgcaacgc agcttatgat
     4201 ttataccatc aacagagaaa ctcctttgaa aactttggaa aaaattccgt tggaggaact
     4261 gaatcgtata gccgaaaaag caaaaagcta cggatttgac gtttctgttt caggataatt
     4321 accaaagccg aagatgaaaa tactcgtttg tcctttaaat tggggattgg gacatgctgc
     4381 acgctgcgtg ccgattattc gtcaactgat tacagaaggc aatgatgtag tgattgcagc
     4441 ggatggtttt ccattaaaat ttcttcaact tgagttcccc gaacttcgat ttttgaaagc
     4501 tcctactcaa aatgttcgtt acagcacggg aaattctcaa atctgggcta tgtttaaaag
     4561 catcccgcgt atttttggca atatttttca agatcattgt tggcttcatc gacttttaaa
     4621 acaggaacat ttcgatcgaa ttatttccga caaccgtttt ggatgttgga gcaaaagaac
     4681 gtattgcatt tacattacac atcaactgat ggtaaaaatg ccgcccgaat tgcgcttgct
     4741 ggaaccgatt gtctggcgac ttcatcgctt tttcattcat cgttacgacg aatgctggat
     4801 tcccgatttg gccggagatg aaaacttttc gggagatttg gcacatcgtt atcctttacc
     4861 cgaaaatgca cattttattg gcattctatc tcgttttcaa caattgaaaa atattattcc
     4921 aaactatact tacgaaacgg tgatggttct ctccggtccg gaaccgcaac gtaccatttt
     4981 cgagaaacaa ctgacagaaa aattcagcaa agacactgtc aaatcgttga tgattcaggg
     5041 attgccacag gaaaaacaaa cttccgtaca agttggcaac ttaaccaaag tttctcatct
     5101 ttccactcaa gaaatggcgt ccttattagc aggcgcaaaa aaaattattg ctcgttcggg
     5161 atactcaacc atcatggatt tgcaagcact caactgcttg gacaaagcaa tactcatccc
     5221 tactccagga cagaccgaac agatttattt ggcagaactg cacaaaaagc gccgataaaa
     5281 cttcaattca ttttgtagcc aacaatcgat tttctgattt caatcagttt tatgagaaaa
     5341 tttttaacca tcaaagaata agataattgg ctctttcatg cattaaaaaa tcaatagata
     5401 cattaaaaaa acggaagcaa aaaaaatttt acctccgttc aaaataaatg ttttaaactt
     5461 gttgtcttta aatcagtttt cgattattcg tgaaacaaaa ttttgcactt ctgcataaga
     5521 aatattcatg attccctgaa tttctttttc cattagaggt gcaaacattg aaatattcat
     5581 ttgagacacg taagtatttc catccgattt ttcgtaaatt gaaattctac acggcatcat
     5641 gctcgaaaaa gtgcgccatt catcttttcc caaaatggaa taggcatgag atggagaaca
     5701 cagttccaac acttttacag gtaaaacttc tttattgttt cttgccatag tttcctgcag
     5761 gtcatgcaca taaaccactt tccatccccc ctcctgaaca atatttgtaa attcagtaat
     5821 ggttttgttg aaactgaatt tgctcttctt ttccaaaaaa aattcttcca ttcctttcgg
     5881 gttgtcttat tgttttactt tttctttctt aagcaatacc tcatcaatgg catttttcag
     5941 cacatctttt ggcaaagcgc cttgagccat ttgcggattc tcattcatag gacagaaaag
     6001 taaagttgga atgctacgaa tgccaaatgc tgcagccagc tcctgttcct tatccgtatc
     6061 aattttataa atgtaaatct gtccatcata ctctttagcc agttcttcca atattggagc
     6121 cactgccttg caaggtccac accagtcggc ataaaaatca atcacgcaag gtttgtcgcc
     6181 cagatatttc cattcgttag ggctggcttc gtaattgact acctttttca aaaattcttc
     6241 tttggttaaa tgaattgttc ccattagttt ttcttcgttt ttgttttgtg aattattctg
     6301 tttgttttct ccattgcttc ccgcacaagc gcttaaagca ataaaagtga tcattaaaaa
     6361 tattccggtc aattttttca tattcttctt atttttggtt tttatttttt ttgtcgaaaa
     6421 ttagttttca aaacgtaaat ttacaagttt ctcatcggat tttaatttat ctcattatag
     6481 cggtaaattc attttccccg ttctccgacg tttttaccag ttgaaacaga gcacgactgc
     6541 ccacagggac ttagatggaa caaagcaatc aaaagcaaaa aactgccgat tatcaaataa
     6601 aaaatttccg aagtggagat aaaagcaatt gccaaaacaa tgccaagaat caattgaatc
     6661 attctgacaa tattccactg aaaaaatatt tttttaatcc tcctgatcgt tcgcattggt
     6721 gtttttactt ttcactttaa acatgtcaaa aaacaagtaa cccaaaacgg ctccatatac
     6781 aatgctaatg tacggattgg aactgatggg acaagttccc gatttacaac caacaaaata
     6841 aaaatacgca aatcctccga tgctccccaa aagcactccg actactttga gccaatgacg
     6901 cgaaagaaat tttttcaaca tagttaatcg aaatttagtt ggtgcactga caagaacgaa
     6961 tacagtccaa aatttgaact atttccttgt ttttcaatga ataataacaa ttctttccat
     7021 cccgacgaca atttaaaatg cctttggcac gcatatcggt gagatgatga gaaagcagcg
     7081 actgttcaca attcaattct tcacttattt gcgaaacatt caattcctct ttctccgaaa
     7141 gcagcgaaat gatacacaaa cgtgtagcgt gtgaaatggc tcttagctta taggctgcct
     7201 cttccatttg gtctatgtta atattgttaa tctgttccat atttcaaaag ttgtgcaaat
     7261 atatgaacat ttattcattt gtctataata agttcagaat ttttaacatt tactctattt
     7321 atttccataa tgttattaaa acaaatacat tgtctctgat ttatggcttt tgtttttaaa
     7381 gaaaaaagta tctttgtgtt ttaaatcaga aattcataca catgacctct tcttcaacca
     7441 taatatgtga aaattttatt gccgagttga atgaattcat tgaatatcaa aagccggacg
     7501 gaatttttgt gctggtggac gaaaatacag aaaagttctg tcttccgctc ctgcttcaat
     7561 cggaaaaact aaaaaattgc caagttataa ccattcccag tggtgacgaa aataaaaaca
     7621 tccataccgc cattgaagtt tggaattact tatcatcaca tggcgcaaca cgccgttcgt
     7681 tgatgattaa cgcaggcggt ggaatggttt ccgatttggg cggttttgca gctgctactt
     7741 tcaagcgcgg aatcagctac atcaatgttt ccaccacctt gcttggagct gtagatgctg
     7801 ccaccggtgg aaaaacaggt atcaacttta tggggttgaa aaacgaaatt ggcgtttttg
     7861 ctccggctga caaggtactg ataaacgtgg attttttcag aacactggac gacaagaatt
     7921 tgcgttcggg ctttgcagaa atgataaaac attcgctgct cgattcgcgc gaacattgga
     7981 aaaatattct gaaatttaat ttggatacga ttgattatga acttttaaaa cacagagtgg
     8041 cggaaagcgt aaaaataaaa gaacaaatag tcgaaaagga ccctaaagaa caaaatctgc
     8101 gaaaggcatt gaatttggga catactttcg gtcatgcttt cgagagtttt tcgtacagtc
     8161 aacatcaacc ggttttgcat ggctatgccg ttatgtacgg acttgtatgc gaattatatc
     8221 tttcacacgt gaaactgaac tttccgaaag aagatttgtt aaagctcgtt acacttgcaa
     8281 aagaaaatta tggaaaaatc tctttttgtt gcgatgatta cgaacaactt tatgaactga
     8341 tgacacatga caagaagaat gtttcggata ccatcaactt cactttattg tcagaggtgg
     8401 gaaaaattga attgaatcaa actgctacaa aaaaggaaat ttttgaatgt ttggattttt
     8461 atcaggaaat gtaaagtgat taaaaacaac attaccaaaa atctcagcat caaatacaaa
     8521 aaatgaaaaa ccaatgaaaa aaactttagt aataggtgcc agcaccaatc cagaacgtta
     8581 tgcctacaaa gcagcgcaac gtttgctcga aaacggccac gaaatagaat tgctcggact
     8641 tcgacccggc gaaattttcg gtaaatccat cgatacggaa aaaaaacaat atcccgacat
     8701 tgatacggta actttgtacg taggtccaaa aaatcagcct gaatattacg attatatcct
     8761 atcactccat ccgaaaagag tggtatttaa tcccggaacc gagaatcctg aatttgaagc
     8821 cctgctggaa gcaaacggca ttcaagcaca ggaagcatgt acgctggtat tgctgagcac
     8881 acaacaatat tgaagaaaat caaaaaacaa caagatttta aaacttcatt tttcaacaaa
     8941 atatttcaat ttttttatga aaatagcatt gattggttac ggaaaaatgg gaaaagccat
     9001 tgaacgaata gcaaaggaac gtgggcacga aattgtttgt atcatcgaca ttgacaattt
     9061 gaatgatttt gaatccgaca aatttaaaag tgccgatgtg gccatagaat tcacgcatcc
     9121 cggcgtagcg atgagcaact acagaaaaac ctttgctgcg ggcattcctt tggtatcggg
     9181 gactacaggc tggacaaacg aattacccgc cttaaaaaag gaagtggaag aaaaaggcta
     9241 cacattcttt tggtcatcaa attttagttt gggggtcaat atttttatgg ccgtaaacaa
     9301 atatctggct tccctgatga atcgtttttc ggattacgat gtggaaataa cggaaatcca
     9361 tcatattcat aagctggatg ctccaagcgg aacagccatc acattggccg aagatatgct
     9421 ggaaaacttg gaaagaaaaa cctgttgggt aaaagaagaa gcaaaacagc cccatgaatt
     9481 ggccataaaa tcaattcgtg aagatgaagt cccgggcatc catattattc gctacgatgc
     9541 ttcatttgat gccatagaat ttaaacatga agccaaaacc cgcgatggtt ttgctctggg
     9601 agctgtaatg gctgccgaat ttgccgtcgg gaaaaaagga tggttaacca tgcaggacct
     9661 gctaaaattt taatattttc tccatttagg aaaatcgaat gctaaaaagt tcaataattt
     9721 ctcacagaac tgaacaaaac aataaaaatt caaaaaactt aacaatttga tttatgactt
     9781 ccgaacctca cacatcccgt ttccatcaac ctcgtcgaca atggataaaa gctgccattt
     9841 ggagtcttat ctacatttta ttcattgctt gggtaggtaa tttttggtgg ttgttgctgc
     9901 ttcctgtcat tttcgacatg tttatcacca aatttattcc ctggacttgg tggaaaaaaa
     9961 caaagaacaa aactcttcgt tctatttaca gttggattga tgccattgta tttgctttgg
    10021 tagcagtata tttcattaat atttacctgt ttcagaatta tcagattcct acctcatcgc
    10081 tcgaaaaaac tttgctggtt ggtgattttc tgtttgtaag caaagccagt tatgggccgc
    10141 gaattcccaa tacaccgctt tcttttccgt tggtgcagaa tacttttccg attatcaaca
    10201 caaaatccta tctggaaaaa ccccaatggg aatataaacg cctgaaagga ttcgatacca
    10261 ttgcacacaa cgatattgtt gttttcaatt ttccaacggg cgacacggtt tgtctaaaac
    10321 agcaaaaccc tgattattac caactttgct actactacgg acgcgatgtg gttcgcagca
    10381 gaaaagacat ttttggagaa atcgtgtatc ggcctgtcga tcgcagagaa aattacgtta
    10441 aacgctgcat tggcttgcca ggcgactggc tgcaaataaa aagcaatcag gtttatatca
    10501 acggagaaaa gcaaaaacca tttccgggcg ttcagttcaa ttatttggta caaaccgatg
    10561 gaacacgttt gagtgatgaa ttactcacca aattgggaat aagcaaggat gaccgtttga
    10621 ttttaaacac ttctcaaaac ccacaggaaa taatggcttt gggtttggat ccaagtttcc
    10681 ctatttatca ttttccgctg acgggtgaaa tgctgcaaaa actgcaacga gttacgggtg
    10741 ttgttaaaat tgtgattgaa ccggaaatgt atggtggcga tgttttccca ttgggcggag
    10801 gtaaaaactg gacccgcgac aattacggac ccatttacat tcccaaaaaa ggagcagtac
    10861 tggaattgaa tgcctacaat tatcccattt atgaacgggt gattcgcacc tatgaaaaca
    10921 acaagctgga atataaaaac ggaatttttt atatcaatga tcgaccggag aaaacctatc
    10981 gtttcaaaat ggattattac tggatgatgg gcgacaatcg gcataattcg gctgattcgc
    11041 gctattgggg atttgttcct gaagaccata tcgtgggacg gccggtagtg gtttggttgt
    11101 cattggataa ggacaaatcg ttgtttggtg gaaaaatcag gtggaacaga tttttcaagg
    11161 atgctcgaag ataaaacgga aaatatatta tcgtcagcaa aaattcgaga aattttttct
    11221 tcggaattac acaactttat ctctgcaatt cgagaggaga aagtgatgct ttctatgtct
    11281 tatctggctc cgatagatta ttttatgact ttgacaaatg cccgatcggc tgtgatcgaa
    11341 caacatgaat attatcagaa acaaacctat cgaaaccgat gcagaattgc cggagcaaat
    11401 ggagtgttgg atctgattat tccggttgaa aaaaccgcaa acaacaaagc tactgttcgc
    11461 gatattcgga tttcgggaca caatgattgg caacgtcaac attggaaagc aatttcttct
    11521 gcttaccttt ccagtccgtt ctttgaatat tatttggatg attttcgccc attttatgaa
    11581 aaaaattggt tatttttgtg ggacttcaac atggaaattc agaacaaaat ttttgaattg
    11641 ctggaaattt atcccaagtt ggaatttacg gacaagttta ttgataaaat tgaagaaaat
    11701 atactggatt tgcgtttgca attccatccc aagaaacagc cctttctaaa aattccttca
    11761 tactatcagg tgttcgaaaa taaattcgga tttatttcgg gactgagtat cattgacttg
    11821 ctgttcaaca tgggtaacga aagtctgtta tggcttttgg gaaaagcaga ataaaaattt
    11881 ttaatgagaa agaagaaact gattttgtgg atttttcaaa aaattaaaac atataaatat
    11941 ggaactggat tggaacaata ttggatttgg ttatgtgaaa accgactaca acattcgttg
    12001 cagttatcaa aatggtgaat ggggagaatt tgaagttcac gaagatgaat acctgacatt
    12061 gcacatggca gctacttgcc tgcattacgg tcaggaagtt tttgaagggt tgaaagcttt
    12121 tagaggcatt gacggaaaaa tcagggtttt tcgcatggat gaaaatgcca aaaggatgca
    12181 atcatcttgc aaaggaattg ttatgcaacc tttccctgtc gatagatttc aggaagccgt
    12241 tcttaaagtt gtcaaactga atgaacgatt tgttccccct tatgagtccg gagcttcgtt
    12301 gtatatccgt ccgcttatga ttggaacaag tgctcaagtt ggcgtcaaac cttcacgcga
    12361 atatcttttt atattatttg taacccctgt aggaccctat ttcaaaggag gctttcagac
    12421 aactccatac gtcattttaa gagagttcga tcgcgcagct ccgctgggaa caggaattta
    12481 taaagtggga ggaaattatg cagcagggct ggctgccgac gaaaaagctc attctttagg
    12541 ttattcggca gttttctatc tggatgccaa agagaaaaaa tatattgacg aatgcggcgc
    12601 tgccaacttt ttcggaatta aaaacaatac ttatgtaacg ccaaaatcga cttccgtgct
    12661 gccttccatc accaataaaa gtttaatgac attggccaga gacatgggca tgaaagtgga
    12721 acagcgtccc gtacccattg aagagctcag cacttttgaa gaagccggag catgtggtac
    12781 ggcagccgtt atcagcccta ttcaacgaat cgacgatttg gacgaaaata aatcctacgt
    12841 tttttcgccg gatggtaaac cgggcgttat ttgcgaaaaa ttatacaaaa aaattcgtgc
    12901 cattcagtat ggcgaagaac ccgatattta tggctggaca accattgtcg aataaatttt
    12961 ctatagaaga tggtcgtttc tttttctaaa aaaattttgt ttgagaaatc aatttaaaca
    13021 aatgtatcaa gcaaaaattt tttactgata attttttaaa atcgaagcac agaaaaatgg
    13081 ttcagttccc ggtaaaaaac atatttacgg tttaatgaag ttatatcaga tcaaaaatta
    13141 atacaagtta tggtggcttt tgaaaaatat tttgttaaaa agaaaagaac attcgttttt
    13201 ttgtcgatct tttttctttt tacaggtgcc aaagccgacg aaggcatgtg gatgttgcct
    13261 tacattgagc agctcaatat tcaaaaaatg aacggaatgg gttgctcgtt gagcgcagaa
    13321 gatatttaca gcgataaaaa tatcagcctt aaagatgccg ttatcgtatt tggaaatggt
    13381 tgtacaggag ttgtggtatc acctcaggga ctgattttta ccaatcatca ctgcggtttt
    13441 gatgccattc aacagcacag ttcggttgaa cataattatt taaaagatgg atttaacgcc
    13501 gtccgattgg aagatgaaat ctccacgccc ggtcttagtg tgaaatttct ggttaaaatc
    13561 gaagatgtaa ccaatcaagt tctttcccat ttgccggata gtttgtccga aaaatcgaga
    13621 aacggaaaaa tttatgaaat ttccgacagt atttgtaaag cagcggaaaa ggacacgcat
    13681 tatctggcag aagtgaaacc tttctttgca ggaaacgaat tttacctgct tgtttacgaa
    13741 gaatttaaag atgttcgact tgcttttgca ccgcccagtt ccattggaaa tttcggaggt
    13801 gataccgata attggatgtg gccacgtcat acgggcgatt tttcggtttt ccgcgtttat
    13861 gcgactcccg acgggaaacc ggccgaatat tcaaaagaca atgttcccta ttctccgaaa
    13921 cgttttgcgt ccgtttccaa caaaggttat caaaccaacg attttaccat gattttgggc
    13981 aatcccggct ctaccagccg ctatttgact tcttttggcg tgaaattcag aatggaaacc
    14041 ggcaatcagg cacgcattga tgtcagaggc atcaagcaga ctatttggcg ttcgtttatg
    14101 cggaaggatg aggcaatcaa cattgcttat gccaataaat atgcttcgag ttccaactat
    14161 tggaaaaaca gcatcggcat gaataaagcc attcagaaat taaatgttct ggagagaaaa
    14221 agggaacagg aagctgcttt tttggaatgg acaaaatcga atccggaaag agagaaaaaa
    14281 tacaaagatg tattgaaaac gttggaagat ggatattcta cgctgtatcc ttatgctcgt
    14341 gcgctcaact atttgcgtga atcattgatg agcggtgtgg aattgcccag aattgcccag
    14401 aaagcaagtc aacttatttc gcaaaacttg ccaaaagatt cgctgatcaa ggaaatagaa
    14461 gaaatttaca aggattaccg tccggaagtt gatcaagccg tttttgctgc catgttggat
    14521 gtttataaaa actcggtaga tgcggatgca cttcctgctt tttatcagga gattcaaaaa
    14581 aaattcaagg gaaatacgac gctttacgca gcttcaattt tccaaaattc cgcctttagt
    14641 tcggcagata aattgaagaa aaatttacag aaaaagaaaa ttaaaattgc caacgatccg
    14701 gcattgattt ttggcaacga agtaagaaat acgtatgaac aaatcagttc gggcatttat
    14761 gctcaaacat ccgaaaaggt gaaagaagct gaacgtattt atttttcagg gcttagggaa
    14821 atgtacgaag aacaagggaa aaaactttat cccgatgcca actttaccat gcgaatgact
    14881 tatgggaaaa tcggaggtta tcagcctgcc gatgctgtgg attaccatta ttacaccaca
    14941 accaaaggga ttttggaaaa agaaaatcca accgatccgg aatttattgt tcccgataag
    15001 ttgaaaaagt cgattctgga aaaagacttt ggaaaatatc tggatcctca gacgaaagac
    15061 atgcatgtaa attttattag caataacgat attacgggag ggaattcggg cagtcccgtt
    15121 ttcaatggga aaggagaatt aattggtttg gcttttgacg gcaactggga agccatgagt
    15181 ggtgatattg tttttgaacc ggagctgcaa cgtaccatca gtgtggatat caggtatgtg
    15241 ctttatgtaa tggacaaaat tggtggcgca cagcgattaa tagatgaatt aagcatatcg
    15301 ggatactaat ttttcagata agctcacttt tttattctac gacaaatatt tacaatgatg
    15361 acaatttatg g
//
LOCUS       DAOTAX010000049        14843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3407_length_14843_cov_25.371044, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000049 DAOTAX010000000
VERSION     DAOTAX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_14843_cov_25.371044"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLM04_06145"
     CDS             <1..518
                     /locus_tag="PLM04_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HON02691.1"
                     /translation="SQTLLDLYSILKTQGTLENLKICVAGDLKYGRTVHSLIMAMSYF
                     HPTFRFIAPEELKLNEEYRIFCLMNNIPFEETTELNDNINDADILYMTRVQRERFQDL
                     MEYERVKNVYTLKNSMLENTKPNLRILHPLPRVSEIAPDVDKNEKAYYFQQAQNGLYI
                     RQAIISKVLSV"
     gene            593..1048
                     /gene="pyrI"
                     /locus_tag="PLM04_06150"
     CDS             593..1048
                     /gene="pyrI"
                     /locus_tag="PLM04_06150"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HON02692.1"
                     /translation="MEKELKVAALRNGTVIDHIPADKLYQVVFMLHLDTCDNQITIGN
                     NLESAKIGKKGIIKIAGKVFAEDETNKIALIAPNAKINIIKDYQVIEKRPLSLPDEIH
                     EIAQCNNPNCITNHQNISTFFNVIKDETGTMLKCHYCERLIKLEEVKIK"
     gene            1126..1755
                     /locus_tag="PLM04_06155"
     CDS             1126..1755
                     /locus_tag="PLM04_06155"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HON02693.1"
                     /translation="MTKIDDENSSHVSWKPGTMIYPLPAVLVSCGKSADEYNLLTVSW
                     VGTICTEPPMCYISIRPERHSYEIIKRNGEFVLNLTTEKLAYATDWCGVKSGKDHRKF
                     EEMKLTPEKGKMVDAPIVKESPVCIECKVKEIVPLGTHHMFIAEVVNIQANPQYIDSK
                     TGAMHLENAHLIAYSHGHYYKLGEEIGKFGWSVRKPGKKKKVSPKIPKK"
     gene            1850..3130
                     /gene="glyA"
                     /locus_tag="PLM04_06160"
     CDS             1850..3130
                     /gene="glyA"
                     /locus_tag="PLM04_06160"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HON02694.1"
                     /translation="MKRDQEIFDIIEREKQRQMKGIELIASENFVSNQVLEAMGSVLT
                     NKYAEGYPGKRYYGGCEVVDESEQLAIDRLKKIFDAEWVNVQPHSGAQANAAVFLACL
                     NPGDKFLGLNLAHGGHLSHGSPVNSSGILYQPLQYNVKADTGRVDYDQMEEIALREKP
                     KLIIGGASAYSREWDYKRMREIADKIGAILMIDMAHPAGLIAAGLLENPLKWAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGKTTPKGEVKMMSALLDSAVFPGIQGGPLEHVIA
                     AKAVAFSECLQPEFFEYQKQVKKNAEKLANELISRGFNIVSGGTDNHCMLVDLRSKFP
                     DLTGKMAENALVKADITVNKNMVPFDSRSPFQTSGIRLGTPAITTRGAKEDLMTEIAE
                     MIETVLQNADNEKIILQVKEKVNKMMKDFPLFAY"
     gene            3263..4258
                     /locus_tag="PLM04_06165"
     CDS             3263..4258
                     /locus_tag="PLM04_06165"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HON02695.1"
                     /translation="MNVKGKIAIIGGGSWATALAKIALSNEQQINWYMRRQEQIDEFI
                     RQSHNPSYLTGVEFDTDRINFTSDINKVVKSSDVLIFATPSPFFKGHLKKLRQKLDKK
                     FVVSAIKGIVPEENMVISEYFEQFYNVSPENMAVLAGPCHAEEVAMERLSYLTVACAS
                     REKARMLADRLATNFIKTSVSDDVLGIEYASVMKNIYAIAAGICHGLKYGDNFQAVLV
                     SNAIREMNRFCNAMNALHRDISESAYLGDLLVTSYSKFSRNRLFGTMIGKGYSVKTAQ
                     MEMEMIAEGYYATKCIHEINEKVQISMPIVDTVFQILYNRCSPIIEIRKLTEQLM"
     gene            4282..5625
                     /locus_tag="PLM04_06170"
     CDS             4282..5625
                     /locus_tag="PLM04_06170"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HON02696.1"
                     /translation="MEHIQFFSDKSSVSKEKIAEYKEKAIRCISALHEGTGKGSDFLG
                     WLHLPSSIDEAQFAEIENTAKLLQKSCEVVVVIGIGGSYLGAKAVIDALSNSLDWLQK
                     VRKSPIIVYAGHNISEDYLFELQEFLKDKTFGIISISKSGTTTEPALAFRLLKTQLEE
                     QQGKAEAKKLIVAITDRSKGALRKLAEQEGYKTFVIEDNIGGRYSVLTPVGLLPIATA
                     GFDIRQLVAGAAFMESLCGMDTPFEKNPAAQYAVVRNELYQQKGKKIEILVNFHPKLH
                     YVSEWWKQLYGESEGKDHKGIFPAAVDFTTDLHSMGQWIQEGERSIFETVISIKEPNH
                     LKTVPFDPENLDGLNYLSGKRVDEVNKMAELGTMIAHVDGGVPNLKVEMPKLNEYYLG
                     ELLYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLEKPGYEQETKAIKEKLK"
     gene            complement(5747..7402)
                     /locus_tag="PLM04_06175"
     CDS             complement(5747..7402)
                     /locus_tag="PLM04_06175"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HON02697.1"
                     /translation="METNKSPLQIARSSYQPKIPTILSGNIALKYGEPTESVADQKEI
                     KNLFPKTYGLPIITFEEGAQVEYPEIAVGVILSGGQAPGGHNVIAGLFDGLKKLNPKN
                     KLYGFLDGPSGLVDHKYTELTSEIIDEYRNTGGFDIIGSGRTKLELEWQFEQGAEICR
                     KLKIKAIVIIGGDDSNTNACVLAEYYARKNFGIQVIGCPKTIDGDLKNEMIETSFGFD
                     TACKVYSELIGNIQRDASSAKKYWHFIRLMGRSASHITLECALQSQPNITLISEEVEG
                     KNLSLSDIVEYIVQIIVQRANHGINYGTVLIPEGLIEFIPGMKKLIQELNDLLANNED
                     FVALHTDDEKRQYIKGMLSPEASQLYRSLPKGIARQLSMDRDPHGNVQVSLIETEKLL
                     IEMVAKRLAQLKAEGVYKGIFSAISHFFGYEGRCAIPSNFDANYTYTLGIVASLLIAE
                     GKTGYMASVKNLTDEPANWIACGVPLTMLMNMEKRNQKMKPVIQKALVKLDGAPFKYL
                     EAHRDEWADEKVSYIYPGPIQYFGPTEVCDLPTKTLLIEHGKL"
     gene            7765..8439
                     /locus_tag="PLM04_06180"
     CDS             7765..8439
                     /locus_tag="PLM04_06180"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HON02698.1"
                     /translation="MCKNSFRNNVLIVFMILPLALQAQDQYLAEIGVTAGNGYYLGDA
                     NEMPFKNSSLVYGSVFRYRIDPRLSVRVEWNHGTVEGKYFNSTGETLNFGNSFNAVDI
                     NGEFNFFDLMQKSNSRNSKKYSTYIFAGLGTTIYANESSNTVTAFSIPFGVGFKYKVS
                     TRFNVNLQWNMRWLSSDDLEAVNDLNNAQRLNGSNPLNKDFFSTVTLGITFDFLKKPC
                     DCKSKW"
     gene            8455..9174
                     /locus_tag="PLM04_06185"
     CDS             8455..9174
                     /locus_tag="PLM04_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HON02699.1"
                     /translation="MFKEKIDYTHLPKHIAIIMDGNGRWAKEKGYNRIYGHQKGVTSV
                     RRITEAAAESNIQFLTLYTFSTENWNRPSDEIKALMELLVITIDKEMEDLNKNNIRML
                     TIGDLSKLPNDAAEKIRECKAKTAQNTGLTLNLALSYSSRWEITEAVKNIVCDVEKGK
                     LTSKNISDKLISSYLSTAGIPDPDLMIRTGGELRISNFLLWQSAYTEFYFTSKYWPEF
                     DKEDFYEAIFDFQQRERRFGR"
     gene            9212..11869
                     /locus_tag="PLM04_06190"
     CDS             9212..11869
                     /locus_tag="PLM04_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HON02700.1"
                     /translation="MKNKIYFILFLIFFHQLSSFSQNSKNDSTSIATENESVPLPVIE
                     YSNTNPKYEIAEITVTGADHYEDFVLIGFSGLSVGQVISVPGPEITEAVKRFWKQGLF
                     SDVKIYATKIENGKVWLNISLKERPRISKINYSGLKKGEIEDLEKKLNLSVGNQITPN
                     ITDRIITVIKKYMEEKGFLNTDVFVYQKDDPEKPGHVIVDIEVNKKIKTSVHKIYVTG
                     NQALTVAQIDKAMKKTNEKNLRNFFRSKKFVKQEYENDKKLIIEKYNEIGYRDAYIVT
                     DSIVPFNEKSVDIYLTINEGKKYYFRNISWIGNTVYPYEFLNEKLGIKKGDLYNYNYL
                     IKRLLTDENDAISKLYQNNGYLFFQIEPVEVHVENDSIDFEIRIYEGKQATINEIGIK
                     GNTRVYEHVIRRELRTKPGQLYSQEDIVRSLRELAQMGHFDQEKMNNAIDIQPDPENG
                     TVDINYVLESKGNDQIEFSAGWGATGVVGSVGLKFSNFAIQNLFNPDTYRIVPQGEGQ
                     TFSLNARTNGQSYSSFSISFLEPWLGGKRPNSLSASVYYSTQTAISNRYLNYINNLSS
                     MYYGGYYGGYYNDYYGNNYNYYQTEVDPDRYMRTLGASLGYGKRLNWPDDYFSFYSEL
                     SFQRFMLNNWYAGYFPMTSGTFNNLSMNLILNRSSIDNPIYTRTGSLFSLGLKFTPPY
                     SVFNGKDYSQEMSNEERYKFIEYHKWTFTGKTFTPLTPNNKLVLMARAQFSYLGYYNK
                     NARSPFETFYMGGDGMSSYTSYGTEYVAMRGYENGSLTPYDTKTGIAAGYLYNKYTME
                     LRYPLSLEQSATIYVLSFVEAGNCFNSISGPNGYNPFNLKRSAGIGVRIFLPMFGMMG
                     IDWGYGFDTVTGATKPSGSQFHFVLGQEL"
     gene            11935..12450
                     /locus_tag="PLM04_06195"
     CDS             11935..12450
                     /locus_tag="PLM04_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HON02701.1"
                     /translation="MKKLIVTFCFLSVFTWLSSAQKFASVDMSYILKNIPAYETANEQ
                     INQVSKKWQSEVEALQKEAEAMYKNYQTELVFMTEEMKKKREEEIVAKEKEAQELKRK
                     YFGPQGELFKKRESLMKPIQDEVYTAIQSISKDRNIEVVFDKSTAMSVVFTTPGIDIS
                     DEVLKQLGYSK"
     gene            12509..13015
                     /locus_tag="PLM04_06200"
     CDS             12509..13015
                     /locus_tag="PLM04_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HON02702.1"
                     /translation="MFKKIAFLLLCVFPISLMGQEVKLGHINSQEILSLMPERTEIEK
                     TLNDLQTQWENELLKMREEYYAKIKEFQDKQATMPESIKQARQSEIVEIEQRINTFNQ
                     TASSDLQKKQQELFAPVIDKVKKAINEVGTENNYTYIFDLSTQSIVYQSPKSNDVTAL
                     VKKKLGLK"
     gene            complement(13072..13368)
                     /locus_tag="PLM04_06205"
     CDS             complement(13072..13368)
                     /locus_tag="PLM04_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HON02703.1"
                     /translation="MNKIEGEKQIVQQMIRLYCNKCHHTSSLCDSCLQLSEYAVSRLE
                     KCPFGERKTSCSSCKIHCYEPQMRKEIRQVMRFSGPRMLFYHPILFLNHYLLKK"
     gene            complement(13401..13874)
                     /locus_tag="PLM04_06210"
     CDS             complement(13401..13874)
                     /locus_tag="PLM04_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HON02704.1"
                     /translation="MERLKTFTKAQFSAMTGGAVDYMVMVFFTEVFHIHYTISIAIGG
                     IVGAVVNFTLNKTWTFKSKELPYKSTGMHQLLKFALVVTNSIVLKSSSTYLITTFLKI
                     DYKFSRIITDLIISIGFNYNLQKHWVFRKISIPENNIGLSENNLPLPDNEYKLPE"
     gene            complement(13879..14568)
                     /locus_tag="PLM04_06215"
     CDS             complement(13879..14568)
                     /locus_tag="PLM04_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HON02705.1"
                     /translation="MDQETKKAESLIAQGRIRTNILHNVEQKSIAYLVQKIPAWMSSD
                     MLTAIGFVGSVIVFLSFVLGAYISRYFLLLGILGFMVSWFGDSLDGRIAYYRNKPRKW
                     YGFSLDITVDWLGIILMGTGFIVYAPSYWKIIGFLFVVLYGWEMITALLRYKITEQYS
                     IDSGSFGPTEVRILISLMLILEVIVKDSILYTSALACLVLLIMNFLETGKILKSADER
                     DKKEKEAKIAQ"
BASE COUNT         5034 a         2625 c         2774 g         4410 t
ORIGIN      
        1 cttcccagac cttgcttgat ttgtattcta ttttgaaaac gcagggaacg ctcgaaaatc
       61 tgaaaatttg tgttgccggc gatttgaaat atggacgtac cgttcattct ttgattatgg
      121 ccatgtcgta tttccaccct acatttcggt ttattgcgcc ggaagaattg aaattgaacg
      181 aggaatatag aattttttgc ctcatgaaca atattccttt tgaagaaacg acagaactta
      241 acgacaatat caacgatgca gatattttgt atatgacccg tgttcaacgc gaaagatttc
      301 aagatttgat ggaatatgaa agagtaaaaa atgtttacac cctgaaaaat tccatgttgg
      361 aaaacaccaa gcccaatttg cgaattttgc atcctctgcc tcgtgtgtcg gaaattgcgc
      421 ccgacgtgga taaaaatgaa aaagcctatt attttcagca ggctcaaaat ggattgtaca
      481 tcagacaagc cattatcagc aaagttttgt cggtctaagt tttgttagcc gattttttac
      541 gattcggaat caaaaatttt tattttgaat tttcaacttt atataaagca tcatggaaaa
      601 agagctcaaa gtggcagcat tgcgtaatgg aacggttatt gaccatattc ctgccgataa
      661 attgtatcag gtggtattca tgttgcactt ggatacctgc gacaatcaga tcacaattgg
      721 caacaatctg gagagtgcca aaataggaaa aaaaggcatc attaaaatag ccggaaaagt
      781 gtttgctgag gacgaaacca ataaaattgc cctcattgct cccaatgcta aaataaacat
      841 tatcaaagac taccaggtga ttgaaaaacg accactttcg ttgcctgacg aaattcatga
      901 aattgctcag tgcaacaatc caaattgcat aaccaatcat caaaatattt ccacattttt
      961 caacgtaatc aaggatgaaa ccggtactat gcttaaatgt cattattgtg aaagactgat
     1021 aaaactggaa gaagttaaaa ttaaatgaaa ttttttttgg ataaataggc attttgaaag
     1081 taatttgaca gccatccaat ttattattat tcaaacattt ctgcaatgac aaaaattgat
     1141 gatgaaaatt cttctcatgt ttcatggaaa ccgggtacaa tgatatatcc gctccctgct
     1201 gttctcgttt cgtgcgggaa atcggccgat gaatataatt tgctgacagt tagttgggtg
     1261 ggaacaattt gtacagagcc tcccatgtgt tatatttcca tacgtcccga acggcattct
     1321 tatgaaatta taaaaagaaa tggcgagttt gtactcaatc ttactacgga aaaattggct
     1381 tatgctaccg actggtgcgg tgtaaaaagt ggaaaagatc atcgcaagtt tgaagagatg
     1441 aaacttacac ccgaaaaggg aaaaatggtt gatgcaccca tagtgaaaga atctcctgta
     1501 tgtatcgaat gtaaagtaaa agaaattgtt ccgcttggaa cgcatcacat gtttattgcc
     1561 gaagtagtta acattcaggc caatccacag tatattgaca gcaaaacggg agccatgcac
     1621 ttggaaaatg cacatttgat agcttattcg cacggacatt attacaaact tggagaagaa
     1681 atcggaaaat ttggttggtc ggtaaggaaa cccggcaaaa aaaagaaagt ttccccaaaa
     1741 attccaaaaa aatagaaaaa actttatcaa catctcagaa tttgtttcgt taaaagaacg
     1801 aaaaacaaca agtgtaattc atatttttta aaataataaa gaacagaaaa tgaaaagaga
     1861 ccaggaaatt tttgacatta tcgaacgcga aaaacagcga cagatgaaag gcattgagtt
     1921 gatagcatcg gaaaattttg tgagcaatca ggtgctggaa gcaatgggat cggttttgac
     1981 caacaaatat gctgagggtt atccgggaaa acgatattat ggcggttgcg aagtggtgga
     2041 cgaatcggaa cagctggcta tcgaccgatt aaaaaaaatc tttgatgccg aatgggtgaa
     2101 cgtacagcca cattcaggtg cgcaagccaa tgctgccgta tttttagcct gccttaatcc
     2161 gggagataaa tttttaggtt taaatcttgc tcacggaggc catttatcac atggttcacc
     2221 ggtcaacagt tcgggaattt tgtatcaacc cttgcaatac aacgtaaaag ccgatacggg
     2281 aagagtcgat tatgaccaaa tggaagaaat tgcgcttcgc gaaaaaccaa aattgataat
     2341 tggtggagct tcagcctatt cacgagaatg ggattataaa agaatgcgcg aaattgccga
     2401 caaaattggt gctattttaa tgattgacat ggctcatcct gcagggctga tagctgccgg
     2461 attacttgaa aatcctttaa aatgggctca cattgtaact tctaccactc ataagacctt
     2521 gcgtggacct cgtggaggaa ttattttgat gggaaaagat tttccaaatc cttggggaaa
     2581 aacgaccccc aaaggagaag taaaaatgat gtcggctttg ctcgattctg cagtttttcc
     2641 cggtattcag ggtggcccgc tcgaacatgt tattgcggcc aaagctgtgg ctttcagtga
     2701 atgtttgcaa ccggaatttt tcgaatatca gaaacaggtt aaaaagaatg cggaaaagct
     2761 ggccaatgaa cttatttcca gaggtttcaa tatcgtttct ggtggtacgg acaatcattg
     2821 tatgctggtt gatttaagga gcaaattccc cgatttgaca ggcaaaatgg ctgaaaatgc
     2881 tttggtaaaa gccgatataa cggtcaacaa aaatatggtt cctttcgata gtcgatctcc
     2941 attccaaact tccggtattc gacttggtac tcctgccata acaacgcgtg gagccaaaga
     3001 agatttgatg actgaaattg cagaaatgat tgaaaccgtt ttacaaaatg cagataacga
     3061 aaaaattatc cttcaggtaa aggagaaagt taataagatg atgaaggatt ttcctttgtt
     3121 tgcctactaa aagtgcataa aaataaggaa ttttttcaaa atactgtttg aattttaaaa
     3181 atgttttgat ttggtaaaaa gaaaaatcaa tatctttgca ggattttgaa tattaatttt
     3241 gtaattattt tgcagcgtgg gaattaacgt taaagggaaa atagccatta ttggcggagg
     3301 aagttgggct acagctctgg caaaaattgc tttgagcaac gaacagcaaa tcaattggta
     3361 tatgcgccga caggagcaga tagatgaatt tattcgccaa agccacaatc catcctatct
     3421 taccggtgtt gaattcgata cagaccgaat taattttacc agcgacataa acaaagtggt
     3481 aaaatcgtcc gatgtgttga tttttgctac tccatctcct ttttttaagg gacatttgaa
     3541 aaaattgcgt caaaagctgg acaaaaaatt cgttgtttca gccatcaagg gaattgtgcc
     3601 ggaagaaaac atggttattt cggaatactt tgagcaattt tacaatgttt cgcccgaaaa
     3661 tatggccgtt ttggccggtc cgtgtcatgc tgaagaagtg gcaatggaaa gactttccta
     3721 tctgaccgtt gcttgcgctt caagagaaaa agcccgtatg ctggccgata ggttggctac
     3781 caattttatt aaaacttcgg ttagcgacga tgtgttggga attgagtatg cttccgtcat
     3841 gaaaaatatt tatgccattg ctgcgggtat ttgtcacgga ttaaaatatg gcgacaattt
     3901 tcaggccgtt ctcgtctcga atgccattcg tgaaatgaat agattctgca atgccatgaa
     3961 cgctcttcat cgcgatattt ccgagtcggc ttatctaggt gatttgttgg ttacgtctta
     4021 ttcaaaattc agcagaaacc gtctgtttgg taccatgatt ggaaaaggtt attccgtaaa
     4081 aacggcacaa atggaaatgg aaatgattgc tgaaggttat tatgccacca aatgtattca
     4141 cgaaattaac gaaaaagttc aaatcagcat gcctattgtc gatacggttt ttcaaatcct
     4201 gtataatcgt tgttctccca ttatcgaaat tcgtaaactg acagaacaat taatgtaaat
     4261 aacaaaaaaa tttttaataa aatggaacat atacaatttt ttagcgacaa gtcttcagtt
     4321 agcaaagaaa aaatcgctga atataaagag aaagccattc ggtgcatttc ggcacttcat
     4381 gaaggaacgg gaaaaggaag cgattttttg ggttggctgc atcttccgag cagtattgat
     4441 gaagctcaat tcgctgaaat agaaaatacc gccaaacttt tgcagaaaag ttgtgaagta
     4501 gtcgtagtaa tcggaatagg tggaagttat ctcggcgcaa aagcggtgat tgatgccttg
     4561 tcgaatagtt tagattggtt gcagaaagtt cgcaaatcac ccattattgt ttatgcgggt
     4621 cataatatca gtgaagatta tttgtttgaa cttcaggaat ttctcaaaga caaaacattt
     4681 ggcatcatca gtatttccaa atcgggcact actactgaac cggcattggc tttccgcctt
     4741 ctcaaaactc aattggaaga acagcaggga aaagccgaag caaaaaaact gattgttgcc
     4801 attacggacc gttctaaagg tgcgttgcgt aaattagccg aacaggaagg ttataaaacc
     4861 tttgtcatag aagacaacat tggcggacgt tattctgtgc tgacacctgt aggcctgctt
     4921 cccatcgcaa ctgccggctt cgacattcgt cagcttgttg caggagcagc ctttatggaa
     4981 tcgctttgtg gaatggatac gccatttgaa aagaatcccg ctgcacaata tgctgtggtg
     5041 agaaatgagc tttatcagca aaaaggcaaa aaaattgaaa ttcttgtcaa ttttcatcct
     5101 aaattgcact atgtttccga atggtggaaa caactttatg gtgaaagtga aggaaaagat
     5161 cacaagggaa tttttccggc tgcagttgat tttacaaccg acctgcattc tatgggacaa
     5221 tggatacagg aaggcgaaag aagtattttt gaaacggtta tttctattaa agagcccaat
     5281 catttaaaaa ctgttccttt tgatcctgaa aatttagatg gtttgaatta tttgtcgggg
     5341 aaacgggtag acgaagtcaa caaaatggca gaacttggca ccatgatcgc tcatgttgat
     5401 ggaggagttc caaacttgaa agtggaaatg ccgaagttga acgagtatta tttgggtgaa
     5461 ttgctttatt tctttgaaaa ggcttgcggt atcagcggtt atttgcttgg tgtaaatcct
     5521 tttgatcaac ccggagtgga agcttataag aaaaatatgt ttgctttgct cgaaaagcct
     5581 ggatatgaac aggaaacgaa agccataaaa gaaaaattga aataagtatt ggatttcaat
     5641 catttataca gttaaggctg cttcaacgag gcagccttaa ctgtataaat taaaatgatt
     5701 tgtctattac gaaaatttta tacaaaaata tattcatcct tgaatgttat aattttccgt
     5761 gttcgattaa taaagttttg gttggtaaat cacacacttc tgttggacca aaatattgaa
     5821 taggtccggg ataaatgtag cttacctttt cgtcagccca ttcatctcga tgggcttcca
     5881 aatatttaaa aggtgcacca tcaagcttta ctaatgcctt ttgtatcacg ggtttcattt
     5941 tttgattcct tttctccata ttcatcaaca tagttaaagg cacaccacaa gcaatccaat
     6001 ttgcaggttc atctgtcaaa tttttcactg aagccatata accggttttt ccttcagcaa
     6061 ttaaaagtga ggcaacaatt cctaatgtat aggtgtagtt ggcgtcaaaa ttggatggga
     6121 tggcacaacg tccttcataa ccaaagaaat gactgatagc agaaaagatt cccttataaa
     6181 caccttccgc tttcaattga gccaaacgtt ttgccaccat ttctatcaat aatttttcag
     6241 tttcaatgag cgacacttga acatttccat gaggatcgcg atccatagaa agctggcgtg
     6301 caattccttt tggtaaactt ctgtaaagtt gacttgcttc agggctcaac atacccttga
     6361 tatattgacg tttttcatca tcggtatgaa gagctacaaa atcttcatta ttagccaaca
     6421 aatcgtttaa ttcctgaata agttttttca ttccgggaat aaactcaatc aatccttcag
     6481 ggataagtac tgttccatag ttgataccat gattggctcg ttgaacgata atttgaacaa
     6541 tatattccac aatatcgctt aaactcagat ttttcccttc aacttcttcg gaaataagtg
     6601 ttatattcgg ctgactttga agggcacatt ccagtgtgat atgcgaagcc gaacgaccca
     6661 tcagacgaat aaaatgccaa tatttttttg cagagctggc atctcgttga atatttccaa
     6721 taagttccga ataaacttta catgctgtgt caaaaccaaa agatgtttca atcatctcgt
     6781 ttttcaaatc accatctatc gttttagggc aacctattac ctgaatacca aaatttttcc
     6841 gtgcgtaata ctcagccaac acgcaagcat tggtgttaga atcatctcct ccgatgatga
     6901 ctatcgcttt tatttttaac tttctgcaaa tctcggctcc ttgttcaaat tgccactcca
     6961 gttctaattt tgttcttcct gaacctataa tatcaaatcc tccagtatta cgatattcat
     7021 cgataatttc cgaagtcagt tcagtatatt tatgatcaac cagaccactt ggaccatcca
     7081 aaaaaccata aagtttattt ttaggattta atttttttaa tccgtcaaag agtcctgcaa
     7141 ttacattatg tccacccgga gcctgcccgc ccgacaaaat aacacccaca gcaatttcgg
     7201 gatattcaac ctgtgcccct tcttcaaaag taataatcgg taatccgtaa gttttaggaa
     7261 ataaattctt tatttctttc tggtcagcaa ccgattcggt aggttcgcca tattttaatg
     7321 caatgtttcc actcagaata gtaggtattt tgggttgata acttgaacgt gcaatctgca
     7381 agggactctt attggtttcc ataaaaaatt ttttaaaggg ttgatatgta aatgtaagtc
     7441 tttttgaaaa tctatttgca aaattaatca taaaatagaa aaacaacaag caaaaagcat
     7501 tcaattttca ataagcacaa atataatttc aacttttaaa tatcattttc gatggtgaaa
     7561 agattaaatt ttgagtaaaa acacaaacta ttcatttgca attttttgtt aaataaatta
     7621 ttgagttttc aatcaactct gctaaattga aagattatca ctatttttgt agctctattc
     7681 tgcctgagga gaaaatttat attcaaaaaa cacgttatat cattactttt taatagtgtc
     7741 ttatcatatt agttacatca aattatgtgc aagaattcct ttaggaataa cgtgctgatt
     7801 gtttttatga ttctacctct tgcattacaa gcgcaagatc aatatctggc agaaattgga
     7861 gtaacagccg gcaacggata ttatttgggc gatgcgaacg aaatgccttt taagaattcc
     7921 agtttggttt atggttctgt ttttcgctat agaattgatc cgcgtttatc cgtacgggta
     7981 gaatggaatc atggtactgt agaagggaaa tattttaatt ctacgggcga aacattaaat
     8041 ttcggcaatt cattcaatgc cgtagatata aacggagaat ttaatttctt tgatttgatg
     8101 caaaaatcaa atagccgtaa cagcaaaaaa tattcaacat atatttttgc cggtttggga
     8161 acaacgattt atgctaacga aagtagcaat acggtaacag ctttcagtat tccgtttgga
     8221 gtcggattta aatataaagt cagcacacgt ttcaatgtaa atttacagtg gaatatgcgt
     8281 tggttaagta gtgatgattt ggaagcagtt aatgatttga acaatgcaca aagattaaat
     8341 ggttccaatc cattgaataa agattttttt tcgactgtta ctttaggaat cacttttgat
     8401 ttcttaaaaa aaccttgcga ttgcaaatcg aaatggtaaa aatgaaaacc atctatgttt
     8461 aaagaaaaaa ttgattatac gcatctgccc aaacatatag ctataataat ggatggcaac
     8521 ggacgttggg caaaagaaaa aggatacaac agaatttacg gacatcagaa aggcgtaact
     8581 tcagttcgta gaattacaga agcagctgca gaatctaaca ttcagtttct tacgctttat
     8641 actttttcca ccgagaactg gaatcgtccc agcgatgaaa taaaagcatt gatggaattg
     8701 ctggtaatta ctattgataa agaaatggaa gatttgaaca aaaacaacat tcgaatgctc
     8761 accattggcg atttgtcgaa attaccaaac gatgccgcag aaaaaatccg tgaatgtaaa
     8821 gctaaaacag ctcaaaatac gggactcacc ttgaatctgg cattaagtta ttcttcccga
     8881 tgggaaatta ccgaagccgt aaaaaatatt gtttgcgatg tagaaaaagg aaaactaact
     8941 tcaaaaaata tttccgacaa actaatttcc tcttatttgt ccactgccgg cattcccgat
     9001 ccagacttaa tgataagaac aggaggtgaa ctaagaatca gcaacttctt gttatggcaa
     9061 tcggcatata ccgaattcta ttttacttcc aaatattggc ctgaattcga taaagaagat
     9121 ttttacgaag ctatatttga ttttcagcaa cgggaacgtc gttttggtag ataaataaat
     9181 gatatataaa aaattgactg attgaatttt tatgaaaaat aaaatttatt ttattctatt
     9241 cttgatattt ttccatcaac tctcttcttt ttcgcaaaat tcgaaaaacg attcgacctc
     9301 tattgcaact gaaaatgaat cagtaccatt gccggttatt gagtattcaa atacaaatcc
     9361 caaatatgaa attgcagaaa ttacggtaac tggggccgac cactacgaag atttcgtatt
     9421 gattgggttt tccggtttat ctgtagggca agtgataagc gttccgggac cggaaattac
     9481 cgaagccgta aaacgatttt ggaaacaggg tcttttttct gatgttaaaa tctatgccac
     9541 caaaatagaa aatggaaaag tatggttgaa tatttcattg aaagagcgtc ccagaatatc
     9601 aaaaatcaat tattccggtt taaaaaaagg tgaaatagaa gatttggaaa aaaaattaaa
     9661 tttatctgtc ggcaatcaaa ttacaccaaa tattacggac cgaataatta cggttataaa
     9721 aaaatacatg gaagaaaaag gtttcttaaa cactgacgtt tttgtttacc aaaaagacga
     9781 tcccgaaaaa ccgggacacg tgattgtgga tatcgaagtg aacaagaaaa ttaaaaccag
     9841 tgtccataag atatatgtaa caggaaatca agctttgaca gttgcacaaa ttgacaaggc
     9901 catgaaaaaa accaacgaaa aaaatttacg caactttttt cgcagcaaaa aattcgtaaa
     9961 acaagaatac gaaaacgaca aaaaactaat tatagaaaaa tataacgaaa tcggttatcg
    10021 ggacgcctat attgttacgg atagtattgt tccctttaac gaaaaaagcg ttgacattta
    10081 tctcaccatc aatgaaggta aaaaatatta tttcagaaat atcagttgga taggtaacac
    10141 ggtttatcct tacgagtttt taaacgagaa actaggaata aaaaagggag acctttacaa
    10201 ttacaattat ttaatcaagc gccttctaac agacgaaaac gatgccataa gcaaattata
    10261 tcagaataac ggctatctgt tttttcaaat tgaaccggta gaagtacatg tagaaaacga
    10321 ttctattgat tttgaaatta gaatttatga aggtaaacaa gctaccatca acgaaattgg
    10381 tataaaagga aacacccgcg tttacgaaca tgtgataaga agagaattgc gaactaaacc
    10441 cggacaattg tacagtcagg aggatatcgt ccgttccttg cgtgaactgg cgcaaatggg
    10501 tcatttcgac caagaaaaaa tgaataatgc cattgatatt caacccgacc ccgaaaatgg
    10561 caccgttgat attaattatg tgctcgaaag caagggaaac gatcaaatag aattttctgc
    10621 cggttgggga gcaacaggag ttgttggtag tgtaggatta aaattcagta attttgccat
    10681 ccaaaattta tttaatcccg atacttatcg tattgtacca cagggagaag gtcaaacttt
    10741 ttctttaaat gcccgcacca acggacaatc ttacagttct ttcagtattt cgtttcttga
    10801 accatggttg ggaggaaaac gccccaattc actttctgcc tcagtatatt attctacaca
    10861 aactgccatt agcaaccgat acctgaatta catcaataat ctttcgagta tgtattatgg
    10921 tggttattac ggaggatatt acaacgatta ctacggcaat aactataatt actatcaaac
    10981 ggaagtagac cccgaccgct atatgcggac actgggggca tccttaggct atggaaaaag
    11041 attgaattgg cctgacgatt acttctcttt ctattccgaa ctttcatttc aacgtttcat
    11101 gctgaacaac tggtatgccg gatattttcc tatgacttcg ggtaccttca acaatctaag
    11161 tatgaatctg atattgaacc gttcttccat agataatccc atttataccc gtaccgggtc
    11221 attgttctca ttaggactta aatttacgcc cccttattct gttttcaatg gcaaagacta
    11281 ttctcaagaa atgagtaacg aagaacgata taaatttata gaatatcaca aatggacatt
    11341 taccggaaaa acttttacgc cacttactcc caacaataaa ttggtactta tggcaagagc
    11401 acagttttcc tatttaggtt attacaacaa aaatgcccga tcaccttttg aaactttcta
    11461 catgggcggc gacggaatgt cgtcctacac cagctatggc accgaatatg tggccatgcg
    11521 tggttacgaa aacggttcac tcacacctta cgataccaaa acagggattg cagcaggtta
    11581 tttgtacaac aaatatacta tggaactgcg ttatccgctt tcgttggaac agtctgctac
    11641 aatttatgtt ttaagttttg ttgaagcagg aaactgtttc aacagtattt ccggtcccaa
    11701 tggttacaac ccatttaatc tgaaacgatc ggcaggtata ggtgtacgca tatttcttcc
    11761 tatgttcggc atgatgggta tagactgggg ttatggattc gatacggtta ccggagcaac
    11821 taaaccaagc ggcagtcagt tccatttcgt tcttggacag gaattataat acattagatt
    11881 ttctggcaaa gaatttgtta taaattaaaa ttattaagat taaatctttc gattatgaaa
    11941 aaattgatag taacattctg ttttttaagt gtattcacat ggttatcttc tgcacagaaa
    12001 tttgcctcag tggatatgag ctatattttg aaaaatattc cggcatacga aactgccaac
    12061 gaacaaatca atcaggtttc aaaaaaatgg cagtcggaag tagaagctct tcagaaagaa
    12121 gctgaagcca tgtataaaaa ttaccaaacc gagttggtat ttatgacaga agagatgaag
    12181 aaaaagcgtg aagaggaaat tgttgcaaaa gaaaaagaag cacaggaact gaaaagaaaa
    12241 tacttcggcc cgcaaggaga acttttcaaa aaaagagaaa gtttgatgaa gcctattcaa
    12301 gatgaagttt acacagccat tcaaagcatc agcaaagatc gaaatataga agtggttttt
    12361 gataaaagca cggccatgag tgtagttttt acaacacccg gcatcgacat tagcgatgag
    12421 gtactcaaac agttgggtta ttcaaagtaa tttaatatat ttgcactctg tttttcaaat
    12481 ttgtataatc aacatcaaaa aaattgtcat gtttaagaaa attgcctttt tattattatg
    12541 tgtcttccct atcagtttaa tgggtcaaga agtaaaatta ggacatatca attcgcagga
    12601 aattctttct ttaatgccgg aaagaaccga aatcgaaaaa actttaaacg atttgcaaac
    12661 gcaatgggaa aatgaactgt taaaaatgag ggaagaatat tacgctaaaa tcaaagaatt
    12721 tcaggacaaa caagccacca tgcccgaaag catcaaacag gctcgccaaa gtgaaattgt
    12781 tgaaatcgaa caacgtatca ataccttcaa ccagactgcc agcagtgatt tgcaaaaaaa
    12841 acagcaggaa ttatttgcac cggtaattga taaggtaaaa aaagcaatca atgaagtggg
    12901 taccgaaaat aattatacgt acatttttga tttaagcaca caatccattg tttatcaatc
    12961 accaaaatcg aatgatgtta ctgctcttgt caaaaagaaa ttgggattga aataatcgag
    13021 aaaattatta cgctagatta tttaagcctt tgatgaccag ctcattgaag gctatttttt
    13081 taacaaataa tgatttaaaa aaagtatcgg atggtaaaac agcatgcgag gacctgaaaa
    13141 tctcatcacc tgacgtattt cttttctcat ttgaggttca tagcaatgta ttttacagga
    13201 cgaacaggaa gtctttcttt ctccaaaagg acatttttcc aaccggctta cagcatattc
    13261 cgacaactgc aaacaagaat cacacaatga agatgtatga tgacatttgt tacagtacaa
    13321 ccgaatcatt tgttgtacaa tctgtttttc gccctcaatc ttgttcattt gaaagaaaaa
    13381 agaaattgat cgaatgaatt ttattcggga agtttatatt cgttatccgg taatggtaag
    13441 ttgttttctg aaagaccgat attattttca ggaatgctga tttttctgaa tacccaatgc
    13501 ttctgcaaat tataattaaa tcctatggat ataattaaat cggtaataat tcggctgaat
    13561 ttataatcga ttttaagaaa tgtagtaatc aaataggttg aagaagattt taaaacaata
    13621 ctgttggtaa ccaccaaagc aaatttgagc aattggtgca ttccggtaga tttataaggg
    13681 agttctttcg atttgaaagt ccaggtttta ttcaatgtaa aatttacgac tgctcccaca
    13741 attccaccaa tggcgatgga aatggtataa tgaatatgaa aaacttcagt gaaaaaaacc
    13801 atcaccatgt agtcgaccgc accaccggtc atggcagaaa actgagcttt ggtaaatgtt
    13861 ttaagcctct ccataaggtt attgagcaat ttttgcttct ttttcttttt tatctcgctc
    13921 atctgcagat tttaatattt tacccgtttc taaaaaattc atgatcaaca ggaccaagca
    13981 agcaagagct gatgtatata aaatggaatc cttgacaatt acttcaagaa taagcattaa
    14041 cgaaatgaga atgcgtactt ctgtaggacc aaaacttccc gagtcaattg aatactgctc
    14101 tgtaatttta tatctgagta aagcggttat catttcccaa ccataaagaa ctacaaacag
    14161 aaatccgatt attttccaat aagaaggcgc ataaacgata aagccggttc ccattaagat
    14221 aattcccagc cagtcgacag ttatatcgag tgaaaaaccg taccatttac gaggtttgtt
    14281 gcgatagtaa gctatccttc cgtccagtga atcaccaaac caacttacca taaatcccaa
    14341 tatgcccaac aacaaaaagt aacgactgat gtaagcaccc agtacaaagg acaaaaatac
    14401 aatcacactc cccacaaaac ctatggctgt aagcatatcg gaactcatcc aagccggaat
    14461 tttttgcacc agataagcaa tcgatttttg ttcgacattg tgaagaatat tggttcgaat
    14521 tcttccctgg gcaattaaac tttcggcttt ttttgtttct tgatccatgt atgaaaatta
    14581 taattaactg attgcgaaga aaatattgaa ttttagaata aaaatccaac acaatttttc
    14641 taaattttta ttctgcaaag ttacgatttt ttttaaaata agaacctaac tgcacctgtt
    14701 tttgaaaata ttttcaaaaa cacaaagaaa acaaaattct tttaaaaaac aaaaaatcag
    14761 ataattttct tcaaagaaag taggacaaga agaaaaacta atccccaaaa aattgctatt
    14821 gaatttaaat ctctactcga ttt
//
LOCUS       DAOTAX010000050        14708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3442_length_14708_cov_30.124753, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000050 DAOTAX010000000
VERSION     DAOTAX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_14708_cov_30.124753"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..2991)
                     /locus_tag="PLM04_06220"
     CDS             complement(145..2991)
                     /locus_tag="PLM04_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HON02706.1"
                     /translation="MKKIHLLFIIFCACAANAQEFIPLVTQFSKNDYRAANQNWAVAQ
                     GLDGILYFGNNEGLLEFDGSLWKLHKIPGNGLVRSVMIDKKNRIYIGAFEEFGYFERD
                     RFGQLNYTSLSALLKNYKMQNDEVWNIFDDQGTIFFQSFTSYFTFKNGEVKGFRTDFS
                     FLFFNLYQNTLYTHTFQKDFCYFDRQQNTFVPVPNNIMKSPVIKILPFDDKHALMVTI
                     SDGLYLYDRDKFTRFATEVDDVLKKAQINRAVISPNGLIVIGTILNGAVAIDKSGRKI
                     WAISSSNVLQNNTVLGITFDRDNNIWLALDKGIAFIHQNSSFRCIQSFYPAIGAIYSV
                     SYYPPHIYIATNQGLYKALLEHHSIRNLSIDPKIKGQVWDVSSFDGQQFCGNNEETFE
                     VTPTRSIPVSPVKGGMCFAHGNIHGVEVLVQGTYTQLCIYKKENGKWKFSHAVEDFLH
                     PIRYIEIDYTGTIWASHLHRGLFAIQLTPDLRKIEHINIYKSLDQQHLNTINVFSVNN
                     RVVFTDYNLFYTFDDIRKKIVPFDELNKALGNFSNSYRICYFKGNLYWFVRPGEAALV
                     EIKPGQIKLVDMVQYADFLHQTVDDYQNIIPISENECIFTLENGLALYNYTQKPIPQY
                     SIQLKLKSIQISDAANKKLNFLPLEATSLPTIPYSRNNVVFSVFYPEYAQRNNVMYRF
                     KLEGLDVVWRDPSSSPSIEYKYLPYRKYVFKAEAISESGQILSETNFAFEVQPPFYLS
                     TPAKIIYTLAVLFLLWGIYWYVQRIIHRKKEKVRIEQEDIRRKEIEKREQQIMTLEKE
                     KLESELTLKSKELATSTMTLIRKNEVLATIKEEIMQQKAALGSQYPNKYYEKIIRLLD
                     ENLSSEDDWAIFQANFDRIHENFFRHLHERYPELTPNDLRFCAYLRLNLSSKDIAHLM
                     NISLKGVEMGRARIRKKIGLPSTKSLTEFMIEFK"
     gene            complement(3392..5101)
                     /locus_tag="PLM04_06225"
     CDS             complement(3392..5101)
                     /locus_tag="PLM04_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HON02707.1"
                     /translation="MMNFYNDNPALKFQLSHPLMQKIVTLKERNFADKDKYDYASRDF
                     EDAIDNYEKILEIIGEICGEVIAPNAEEVDHTGPQVVNNRVVYAPGTQQNHEVLTQAS
                     LYGIGLPRQYGGLNFSMVPYVMAAEMVSRADAGFANIWGLQDCAETINEFADEDLKRE
                     FLPRVCQGQTCSMDLTEPDAGSDLQAVQLKATFNEKDGIWYLNGVKRFITNGDAHIKL
                     VLARSEEGTTDGRGLSLFLVDNKHDDTVKVRRIENKLGIKGSPTCELVFSNTPAKLIG
                     SRRLGLIKYVMSLMNSARLGVGAQSVGLSASAYNEALKYAKERQQFGKAIIEFPAVFE
                     MISLIKAKLDASRALLYETTRFVDMYKAYEAIEKERRLEPEEKAEYKEFQRLADAFTP
                     LLKMFASEYANQNAFDCVQIHGGSGFMKDYACERLYRDARILTIYEGTSQLQVVAAIR
                     HVTTGTYLQQIREYEKLPINPEFHSFRKRLMIMTNLYENSVSRVIETKNQEYIDFQAR
                     RLVEMAGHIIMGYLLILDATRNDMFRKSAEVYINYGEVETRKHATFIKTFDMENIDIY
                     KVV"
     gene            complement(5215..6234)
                     /locus_tag="PLM04_06230"
     CDS             complement(5215..6234)
                     /locus_tag="PLM04_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HON02708.1"
                     /translation="MNNLFVYLEIEDGIVKDVSLELLTKGRSLAKQLNCKLEAVAIGY
                     QLENIPKQVFPYGVEVLHLADDKRFVPYTTQPHASVLIHLFKEEKPQIALLGATSLGR
                     DLGPRVSSALFSGLTADCTSLEIGDYEDKKAGKVYKNLLYQIRPAFGGNIVATIVNPD
                     CRPQMATVREGVMKKEIFDPKHTGEIRRLEVSKYVSDQDFVIEVIERHIEKTKIDIKS
                     APIIVSGGYGVGSAENFKLLYELAEVLGGEVGASRAAVDAGFAEHERQIGQTGTTVRP
                     KLYIACGISGQIQHIAGMQESAMIIAINSDPNAPINKIADYVISGRVEDVLPKMIKYY
                     KKNTK"
     gene            complement(6324..7196)
                     /locus_tag="PLM04_06235"
     CDS             complement(6324..7196)
                     /locus_tag="PLM04_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HON02709.1"
                     /translation="MSFNIVVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRIKEKVPGTVVHLLTMGPVRAAEIIRESLYRGADDGFLLSGREFAGSDTLAT
                     SYALSCAIRKLGKIDLILCGRQAIDGDTAQVGPQVAEKLGLPQITYAEDIIEITKKNI
                     TVKRRLERGVEIVKGPLPILVTVNGTAAPCRPRNAKLTLKYKHAATPSEKQESGSDYT
                     DLYDAHPYLNITEWGANDIEHDPKWLGLSGSPTKVKKIDNIVFTAKESKRLTGSDFEI
                     ESLMKELIANHTIG"
     gene            complement(7845..10484)
                     /locus_tag="PLM04_06240"
     CDS             complement(7845..10484)
                     /locus_tag="PLM04_06240"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HON02710.1"
                     /translation="MELPSKYNPEGIESKWYQYWLKNGFFHSTPDQRQPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMQGKNACWVPGTDHASIATEAKVVEKLAAEGIKK
                     TDLSREEFLNHAWEWTRKHGGIILEQLKKLGASCDWDRTAFTMDEIRSQSVIKVFVDL
                     YEKGKIYRGIRMINWDPKAKTALSDEEVIFKEQEGKLYYLKYKLCPTTENDNSDEYLI
                     VATTRPETIMGDTAVCIHPADPRYQHLKGRKCIVPLVNREIPIIEDEYVDMEFGTGCL
                     KVTPAHDVNDYMLGEKHHLPSIDIFNDDGTLNENGGSYAGMDRFEVRKKIVKDLEVAH
                     LLEKTENYINKVGFSERTDVPIEPKLSMQWFLKMEELAKPALEAVMTDKIKLYPPKYK
                     NTYRHWMENVKDWCISRQLWWGHRIPAYYLPNGGWVVAETKEEAYQKALKIANEPFPI
                     ENLRQDEDCLDTWFSSWLWPISVFNGILEPDNEEINYYYPTDDLVTAPEILFFWVARM
                     IIAGYEYRGEKPFKNVYLTGIVRDKLGRKMSKSLGNSPDPLLLIEQYGADAVRMGMLL
                     TSPAGNDLLFDESLCEQGRNFNNKIWNAFRLVKGWQTAEIEQPEASRLAVEWFRHQLN
                     KSLYEMEDLFGKYRLSEALMTVYKLFWDEFASWYLEIVKPGYGQPVDLKTYNATLEFF
                     EVLLKILHPFMPFITEELWQQLSKRKDGESIMMQPLPQPENFDENIIKEFETVKEIVA
                     GIRSVRLQKNIPNKEVLKLLVNGKDNTNFYSVVKKLGNIESIEKTAEKSPSSVSYMVG
                     TTEYIIPTGNKVDIASEIKKLEEELAYLEGFLASVLKKLNNEKFVANAQPQVVENERN
                     KKADAESKIASLRESIDALRKIS"
     gene            10942..13230
                     /locus_tag="PLM04_06245"
     CDS             10942..13230
                     /locus_tag="PLM04_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HON02711.1"
                     /translation="MKKINLLFIVLSLVAVVNAQNKPATPQLGKDSLKEVIKAMTLED
                     KIYMVMGLGEESWKNPASGPKTVLLAGAAGRTWDIPRLGITPAVLTDGPAGLRIEPKQ
                     EGFSHPFYCTAFPTATVLASTWNTPLVEEVGKAMGNEVLEYGSDVLLAPALNIQRNPL
                     CGRNFEYYSEDPLIAGKMAVAMVVGLQSNGVGTSVKHFAANNIETKRTTINAIVSQRA
                     LREIYLRGFEIAVKEGNPWTVMSSYNRLNGFYTSQNPDLLTTILRKEWGFKGMVMSDW
                     WGGDDAVAQMKAGNDLLEPGATQREVLREALKNKTLSEKTLDRNLMRILDFIQKTPHF
                     KGYQPSYHPDLEAHAAIARKAAAEGMVLLKNENSTLPFKKVNKIALFGKTSYQFMTGG
                     TGSGEVNYDHAAALDEAFGTAKFQLSKTLTDRYRQFIDSVRQHEKKENQKFAVDFAPE
                     LNLTKDFIQSQVKNSDIAVITIGRQGGEGWDRPESTFSLSEVEKNLIKNVCTSFHAAK
                     KKVVVVLNIGGVIETADWRDWPDAVLLAWQPGQQGAHAVVDLLKGTENPSGKLAVSFP
                     MKYADVPSAATFPGEPKDNPVNAFYTEGIYVGYRYYETFKVPTAYPFGYGLSYTSFKY
                     SDLKLSNTLFDNEISVSVKITNIGKTAGKEVVQLYREAPKQAIDKPVKELKAFAKTQL
                     LKPNESEVITFKLDARSLASFWSGVSAWIADEGKYKIHVGASSEDIRATGEFSLPKKI
                     MVEKTHDVLYPNLLLHEISPEK"
     gene            13342..14022
                     /locus_tag="PLM04_06250"
     CDS             13342..14022
                     /locus_tag="PLM04_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HON02712.1"
                     /translation="MIQLEALYKISYGLYIVTSGDRNQGNGFVSNTVFQVTSQPQRFA
                     VCCNKDNYSASFIQQFKAFAVSILAQDTSAELIGKFGYKSGREMNKMTDVKVIFKETN
                     VPIVLDSSLAFFECKLVDVFDVGTHWIFIGELVSAEIVDDTGEPLTYAYYREVKKGFS
                     PKNAPTYIDQSKIETKTENAMSKKFKCPICGYIYDEAEEKVKFSDLPDDWVCPVCGSE
                     KSEFIEIS"
     gene            14059..14637
                     /locus_tag="PLM04_06255"
     CDS             14059..14637
                     /locus_tag="PLM04_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HON02713.1"
                     /translation="MDKSLKGTRTEQNLLKAFAGESQARNRYEFFASVAKKEGYEQIA
                     AIFMETASQEKEHAKRFFKFLEGGMVEITASYPAGIIGTTKQNLKAAAEGEYEEWSSL
                     YPTFAEVAKEEGFPEISVAFKMVAKVEVEHERRYLKLLQNISEDKVFKKDGKVLWQCR
                     NCGYIYESEEALEVCPVCLHPKAFMQLKEDNY"
BASE COUNT         4334 a         2842 c         2872 g         4660 t
ORIGIN      
        1 gcaaatcctc ctaccatcca aaaccttatt cgcttattga aaatctggag attttatttt
       61 taactcgaag tagctttgag gctacgccga acggctggta aaccgcataa tagcaaggta
      121 cctttttgtg aaaattgaca gttattattt aaattcaatc ataaattcgg tgagactttt
      181 tgttgaaggg agtccaattt ttttgcggat gcgggctctt cccatttcta cgcctttgag
      241 cgaaatattc attaaatggg caatgtcctt gctcgataga ttgaggcgaa ggtaagcaca
      301 aaatcttaaa tcgttgggtg tgagttcggg atatcgttcg tgtagatgtc ggaaaaagtt
      361 ttcgtgaatt cggtcgaaat tggcctgaaa aattgcccaa tcatcttctg aagaaagatt
      421 ttcgtcgagc agccgaatga ttttttcata atacttgttg gggtattgtg atcctaaagc
      481 tgctttttgt tgcatgattt cttcttttat ggtggccaac acttcatttt ttctgattag
      541 cgtcatggtc gaagtggcca attccttgct cttcagcgtc aattccgatt ccagcttttc
      601 tttttctaat gtcatgattt gctgttctct tttttctatt tcttttcgcc tgatgtcttc
      661 ctgttcgatt ctgacctttt cctttttcct gtgaataatt cgttgaacat accagtagat
      721 tccccaaagt aaaaatagca ccgccaaagt gtagataatt tttgcaggcg tactcaaata
      781 aaagggtggt tgcacttcaa atgcaaaatt tgtttctgag agaatttgtc cactttcgga
      841 tattgcttcg gctttgaata catatttccg atagggcaaa tatttatatt cgatactggg
      901 cgatgaagat ggatctctcc aaaccacgtc caatccttcc agtttaaaac gatacatcac
      961 attgtttctt tgagcatact cggggtaaaa gaccgaaaaa acaacattgt ttctggaata
     1021 aggaatagtc ggtaaactgg tggcttcgag cggtaaaaag ttcaatttct tgttggctgc
     1081 atccgaaatt tgaatgcttt tcagttttaa ctgtatgctg tactgtggaa ttggtttttg
     1141 tgtgtagtta taaagtgcca gtccattctc taaagtgaaa atacattcat tttctgagat
     1201 aggaatgata ttttgataat cgtcaacggt ttgatgaaga aaatcggcat attggaccat
     1261 atccaccaat ttgatttgac cgggtttgat ttcaaccagt gcagcttcgc ccgggcgaac
     1321 aaaccaatac agatttcctt tgaaatagca tatccgatag gaattggaaa aatttccgag
     1381 cgctttgttt aattcgtcga aaggcacaat ttttttacgt atatcgtcga aagtataaaa
     1441 taaattatag tctgtaaata ctactctgtt gtttacagaa aagacgttaa tggtgtttaa
     1501 atgttgctga tcaagagatt tgtatatgtt tatgtgttca attttccgta agtcgggcgt
     1561 aagttgaatg gcaaaaagcc cgcgatgcaa atgacttgcc caaatggttc cggtgtaatc
     1621 tatttcgatg tagcgaatgg gatgaagaaa atcttccact gcatggctga atttccattt
     1681 cccattctct tttttgtaaa tacacagttg cgtataggtt ccctgaacca atacctccac
     1741 accatgaatg tttccatgag cgaaacacat gcctcctttt acgggtgaaa caggaatgga
     1801 ccttgtgggt gtaacttcaa aggtttcttc attgttgcca caaaattgct gaccatcaaa
     1861 ggaacttaca tcccaaactt gtccttttat ttttgggtcg atggaaaggt tacgaatgga
     1921 gtgatgttcc aacaatgctt tgtataatcc ctgattggtt gcaatataaa tatgtggagg
     1981 ataataactt accgaataaa tggcgccaat tgccgggtaa aaggattgaa tgcagcgaaa
     2041 ggatgagttt tgatgaataa aagcaattcc tttatccaac gccagccaaa tattgttgtc
     2101 gcggtcgaaa gtaattccca gaacggtatt gttttgaagg acatttgaag aactgattgc
     2161 ccagatcttt cgtcccgact tgtctatagc cacagctcca tttaaaatgg taccgatcac
     2221 aatcaatccg tttggcgaaa taacggctct gttgatttgt gcttttttca gtacatcgtc
     2281 cacttcagtg gcaaaacggg taaacttatc tctatcataa agataaagac catcggaaat
     2341 cgtaaccatt aaagcatgtt tatcgtcaaa aggcagaatt ttgattaccg gacttttcat
     2401 gatattgttg ggaactggaa caaaagtatt ttgttgccgg tcgaagtagc aaaaatcttt
     2461 ctgaaaagta tgtgtgtata aagtattttg atagaggttg aagaacaaga aggagaaatc
     2521 ggttctgaat ccttttactt ctccgttttt aaatgtaaaa tatgaagtaa aagattgaaa
     2581 aaaaatggtg ccctgatcgt caaagatatt ccaaacttca tcgttctgca ttttgtaatt
     2641 ttttagcaag gcagacaggg aagtataatt taattgtcca aaacggtctc tctcaaaata
     2701 gccaaattct tcaaaagcgc caatgtaaat tctgtttttt ttgtcgatca taacagaacg
     2761 caccagacca tttccgggaa ttttgtgcaa tttccacagg gaaccgtcaa attcgagcag
     2821 tccttcgttg ttcccaaaat aaaggatgcc gtccaaacct tgtgctaccg cccaattttg
     2881 attggcagcg cgataatcgt tttttgaaaa ttgagtaacg agcggaataa attcctgagc
     2941 atttgctgcg caagcacaga aaataatgaa aagcagatgg atttttttca taaaggtgca
     3001 catgtcaatt tttcaaggta ttaaccggag ttctggtcaa aaagttcata agattataaa
     3061 atgatttttt cgacaaaatc aatccgtaaa ttcttgcagg aacatcagaa attaagaatc
     3121 tcaaaaataa gaaaatattt gttgactcaa gagttaataa ggatattttt tagaaataac
     3181 cggcttaata aaaaaaaata aactgaattt ggcaatctca ttcttaaatt cagattttaa
     3241 tttttaaaaa tactttttat caacgcaact atttaattac caattatata gaaacatttt
     3301 taatcatttt aaaataagtc aaagctattt caaaatacaa acgcccaaag ttttttaacc
     3361 ctgagcgttt gctaagaatg taatgataaa ctcaaactac tttgtaaata tcgatgtttt
     3421 ccatgtcgaa agttttgatg aaagtagcat gttttctggt ttcaacttca ccataattga
     3481 tatatacttc agccgatttg cggaacatat cgtttcgggt agcgtcgaga atcagcaaat
     3541 aacccatgat aatgtgccct gccatttcta ccagacgtcg agcctgaaaa tcgatatatt
     3601 cctgattttt agtttcgata acacggctca ccgaattttc atacaggttg gtcataatca
     3661 tcaatctttt tctgaaagaa tgaaattcgg gattgatggg taatttttca tattcacgta
     3721 tttgctgaag ataagttccg gtagtaacgt ggcggatggc agctacaacc tgtaattggg
     3781 aagttccttc atatatggtc agaatacgtg catcgcgata aagtcgttcg caggcatagt
     3841 ctttcataaa tcccgaaccg ccatgaatct gtacacagtc gaaagcattc tggttggcat
     3901 attcgctggc aaacattttc aaaagtggag taaaagcatc ggccagacgc tgaaattcct
     3961 tgtattcggc tttttcttca ggttcgagac ggcgctcttt ttcaatggct tcgtacgctt
     4021 tgtacatatc gacaaaacgg gtagtttcgt acaacaaggc acgtgaagca tcgagcttgg
     4081 cctttataag tgaaatcatc tcaaaaacag caggaaattc aataatggct tttccaaact
     4141 gctgacgttc tttggcatac ttcaaagctt cattatatgc tgaagccgaa agtcccaccg
     4201 attgggctcc tacgccaagt cgggcactat tcatgagcga cataacgtat tttatcaatc
     4261 ccaagcgtct ggaaccaatg agtttcgccg gcgtattgct aaaaaccagt tcgcaggttg
     4321 gagagccctt gatgcccagt ttgttttcga tacggcgaac ttttactgta tcatcgtgtt
     4381 tgttgtccac aaggaaaagc gacaatccac gaccatcggt agtcccttct tcggaacggg
     4441 ccagcaccag tttaatgtga gcatcgccat tggtaataaa acgcttaact ccattaagat
     4501 accaaattcc gtccttttcg ttaaaggtag ctttcagttg cacagcctgc aaatcggaac
     4561 cagcatcagg ttcagtcaaa tccattgaac aggtttgtcc ctgacatact ctcggaagaa
     4621 attcccgttt taagtcttcg tctgcaaatt cgttgatagt ttcagcgcag tcctgcaaac
     4681 cccaaatatt ggcaaaaccc gcatcggcac gcgaaaccat ttcagcagcc attacgtacg
     4741 gcaccatcga aaaatttaat cctccatact gacgtggcaa tccaattccg taaaggctgg
     4801 cttgcgtcag aacttcatgg ttttgttgtg tcccgggagc ataaacaaca cggttgttga
     4861 caacttgagg ccctgtatga tctacttctt cggcattggg agctatcact tctccacaaa
     4921 tttcgccgat aatttccaaa attttttcat aattatcaat ggcatcttca aaatcgcgtg
     4981 aagcataatc atatttgtct ttgtcggcaa aatttctttc cttaagtgta acaattttct
     5041 gcatcagggg atgcgaaagt tggaatttta atgcgggatt atcgttataa aagttcataa
     5101 ttttacggtt attaaattaa gaaaacaatt gaagcgcaaa taataacagg aaacgtttaa
     5161 ataaatttta ttgattcgat tttctgaaat tttttcattc gctgggtttg tttcttattt
     5221 tgtgtttttc ttataatatt ttatcatttt cggaagcaca tcttccactc ttccggagat
     5281 tacgtaatcg gcaattttat tgataggtgc attgggatca ctgttgatgg caataatcat
     5341 ggcactttcc tgcattccgg caatgtgctg aatctgtcca gaaatgccac aggcaatata
     5401 cagttttgga cgtacggtcg ttccggtttg tcctatttga cgttcgtgtt cggcaaaacc
     5461 tgcatcaaca gcagcacgag aagccccaac ttctcctccc aaaacttctg ccagctcata
     5521 aagcaatttg aaattttcgg cacttcctac tccatatcct ccggaaacaa taattggagc
     5581 cgacttaata tcaattttag ttttttcgat atgtcgttca atcacctcaa ttacaaagtc
     5641 ttggtccgaa acgtatttcg aaacttccaa tcgacgaatt tcgcccgtat gttttggatc
     5701 gaaaatctcc tttttcatta cgccttcgcg tacggttgcc atttgtggcc tgcaatcggg
     5761 gttcacaata gtagcaacta tattgccacc aaaagccgga cgaatctgat aaagtaaatt
     5821 cttatacact ttaccggctt ttttgtcttc atagtcgcca atttcgagag aagtacagtc
     5881 ggcggtcaat ccgctgaaaa gagcagaaga tactcttgga cccaaatcgc gcccaagtga
     5941 agtagcacca agaagtgcaa tttgtggttt ttcttcctta aaaagatgaa ttagcaccga
     6001 agcatgaggt tgtgtggtgt aaggaacaaa tcttttatcg tcagccaaat gcaatacctc
     6061 tactccataa ggaaatacct gtttaggtat attttccagc tgataaccaa ttgctacagc
     6121 ttccagcttg caattgagct gttttgccaa cgaacgtccc tttgtaagca attcgaggct
     6181 tacatctttc actattccgt cttctatttc aagatataca aataagttgt tcatatatat
     6241 ttaattcttt tttcctgaaa ggaaattttc tatttttttt gccaataaat tagattagaa
     6301 aaatttttta gagatactca aaattaaccg atagtatggt tagcaatgag ttctttcatt
     6361 aaactttcaa tttcaaagtc gctgcctgtg agtcgtttac tttcttttgc cgtgaaaacg
     6421 atattgtcga ttttcttaac tttagtaggt gaaccgctca atccaagcca ctttggatcg
     6481 tgttcaatgt cattggcacc ccattcggta atgttcaaat agggatgtgc atcgtacaga
     6541 tcggtataat cagaacccga ttcctgtttt tcggatgggg ttgcagcatg tttgtattta
     6601 agtgtcaatt tggcatttct tgggcggcat ggagctgctg ttccgttaac ggtaaccaat
     6661 ataggcagtg gccctttgac aatttccacg cctctttcca gtcggcgttt tacggtgata
     6721 tttttcttgg taatttcaat aatgtcttcg gcgtatgtga tttgtggtaa gcccagtttt
     6781 tccgcaacct gaggccccac ttgcgccgta tctccatcta tggcttgacg gccgcaaagg
     6841 atcagatcga tttttcccaa cttgcgaatg gcgcaggata aagcatagga agttgccaac
     6901 gtatcggaac cggcaaattc tcttccactt aaaagaaatc cgtcgtcagc cccgcgatag
     6961 agactttcac gaatgatttc ggcagcacga accggaccca ttgtcagcaa atgaacaact
     7021 gtgccgggaa ccttttcttt aatgcgaagt gcctgctcaa gagcattcaa atcttccgga
     7081 ttgaaaatag ccggcagcgc agcacgattc actgttccat cggctttcat ggcatctttt
     7141 cccacatttc gggtatcagg cacctgtttg gctaaaacta cgatattgaa actcatttta
     7201 tcattaaatt attgtaatta atattagtct ttcttcttca actgcgaaaa gttttttgtt
     7261 acagcaaagc tgaagaattt tatatccgat tttttttgat ttgcaaaatt agtcgttttt
     7321 attgaagttg caatttgttg tctctaatat aaaatttgag gcctttattc aataaaaatc
     7381 gcgagacatt tcacaaggcc tcgcgttttt taataggtat tagttttaaa ggtataaaaa
     7441 agattgtgtt gtttagttaa cgcttgcaaa atacgcatta aaaaataaaa cctgcaagtc
     7501 aaaatttatg ttttacaacc tatttttgga gcaattcgac ttttaacaat tttcagataa
     7561 ttatatttta tgaacttaat ctattaattt aaagacactt aaattgtctt tggcttgaaa
     7621 tgttaaaaat attaccgttc tatcgaatca taaacaagta ataagttttc taaaagtcga
     7681 acttcttttt tataccgctt gaaccaataa ttttaaaaaa cgtcgaagcg catcttcgat
     7741 aaaaataaga aatatctcaa aaattataaa atcgatgtgg aatccatctc aaaatttgct
     7801 aataaatttt ttaatagctg aaattgaatg gtgacaaagt ctgatcaaga aatttttcgt
     7861 aaagcgtcaa ttgattccct caaagaagct attttgctct cagcatcggc ttttttattt
     7921 cgctcattct caacaacttg aggttgagca ttggcaacaa acttttcgtt gttcagtttt
     7981 ttcaacaccg aagccagaaa accttcgaga taagccaatt cttcttccaa cttctttatt
     8041 tcggaagcta tatccacttt gttgcctgtc gggattatgt attcggtcgt tccaaccata
     8101 taagaaacag agctggggga tttttccgct gttttttcta tcgattcgat atttccgagc
     8161 ttttttacga ccgaataaaa atttgtattg tcttttccgt taactagtaa ctttaaaact
     8221 tctttgttgg gaatattttt ttgtaaacgt acactgcgaa ttccggccac aatttcctta
     8281 acggtttcaa attcctttat tatattctca tcaaagtttt caggttgagg aaggggctgc
     8341 atcatgatac tttcaccatc ttttcttttg gacagctgtt gccacaactc ttccgtgata
     8401 aaaggcatga agggatgcaa tatttttaat agaacctcaa aaaattccaa cgtagcattg
     8461 tatgttttga ggtcaacagg ttgcccgtat cccggtttca caatttccaa ataccatgaa
     8521 gcaaattcgt cccagaaaag cttgtaaacc gtcattaatg cttccgacaa acgatatttg
     8581 ccgaacaaat cttccatttc atacaacgat ttgttgagtt gatgccggaa ccattccact
     8641 gcaagccgtg aggcttcagg ttgttcaatt tcggcggttt gccagccttt taccagtctg
     8701 aatgcattcc agatcttgtt gttgaaattt cttccttgtt cacacaaact ttcgtcaaaa
     8761 agtaaatcgt ttccggccgg ggaagtcaac aacattccca tacggacggc atcagctcca
     8821 tattgttcta tcaaaagcag cggatcgggt gaattcccca gcgatttgga cattttcctt
     8881 cccaatttgt ctcttacaat accggtcaga taaacgtttt taaaaggttt ttcgcctctg
     8941 tattcatatc cggctatgat catgcgtgca acccagaaaa ataaaatttc cggagccgta
     9001 accaaatcat cagtgggata ataatagtta atttcttcat tgtcgggttc gagaatacca
     9061 ttgaaaaccg atattggcca caaccacgaa gaaaaccatg tatccaggca gtcttcgtcc
     9121 tgtcttaaat tctcaatcgg aaatggttcg ttcgctattt tgagtgcttt ttgataagct
     9181 tcttcttttg tttcagcaac tacccaacca ccattcggaa ggtaataggc tgggatgcga
     9241 tgcccccacc aaagttgtcg ggaaatacac cagtctttta cgttttccat ccaatggcga
     9301 taagtatttt tgtattttgg aggatacaat tttattttat cagtcattac ggcttccagt
     9361 gcaggtttgg caagttcttc cattttcagg aaccattgca tggataattt tggttctatg
     9421 ggaacatccg tacgttcaga aaaaccaact ttattgatgt agttttccgt tttttcgagt
     9481 aaatgggcta cttcaaggtc ttttacaatt tttttgcgga cttcaaatcg atccatgccg
     9541 gcgtagctac caccattttc attgagcgtg ccgtcatcat taaaaatatc aatggaaggc
     9601 aaatgatgct tttcgcccag catgtagtcg tttacgtcat gagcgggagt aactttaaga
     9661 caaccagttc caaactccat gtcaacatat tcgtcttcaa taattggaat ttcacggttt
     9721 accaacggaa caatacattt tctacctttc aaatgttggt agcggggatc ggcaggatgg
     9781 atgcaaactg ccgtatctcc catgatggtt tcagggcgtg tggttgcaac aatcagatat
     9841 tcatcactgt tatcgttttc ggtagtcgga caaagtttat atttcaagta atacagtttt
     9901 ccttcctgtt ctttaaaaat tacttcttcg tccgacaaag cggtttttgc tttcgggtcc
     9961 caatttatca ttctaatgcc gcgataaatc tttccctttt cgtataaatc cacaaaaact
    10021 ttgattacac tttgtgaacg gatttcgtcc attgtaaaag ctgtacgatc ccaatcgcat
    10081 gaagctccca gtttttttag ctgttccaga ataatgccgc catgtttccg cgtccattcc
    10141 caagcatgat tcaaaaactc ttcacgtgaa aggtcggttt ttttaattcc ttcggctgca
    10201 agcttttcca caactttagc ttcggtggct atggaagcat gatcggttcc cggcacccaa
    10261 caggcatttt ttccctgcat gcgagcccga cgtaccaaaa tatcctgaat ggtattgttg
    10321 agcatgtgtc ccatgtgaag tactcccgtt acattgggag gcggaatgac aattgtgtaa
    10381 ggttgacgtt gatcgggtgt tgaatgaaaa aaaccatttt tcaaccaata ttgataccat
    10441 tttgattcaa ttccttcggg attgtatttg ctcggtagtt ccatttttta tacttttaca
    10501 atttcaaagt gatgctgctc gatgagttgt gaaaatttaa ttatttgtct atcaaattga
    10561 tagtgaaagt ctttgtctta tttttttgaa atgcaaagat aatcaaaatt tgcaccaact
    10621 gacgaatttt tagaccgaaa aaatttgaat cgagaagctg aaccttaagg cgaacaaagg
    10681 cccgttctaa tttatttgag atgagcaatt ttaatgtcag ttgtattttg aaatgataac
    10741 ggtagatata ggatttggca gaaagacgta aaaagtacgg taaaattttt atcgaacagt
    10801 tattttatac ggttttttaa cctttcaaca ggaacgattt tatatattcg gtattttttc
    10861 agaaaatcca ttcctttctc atttaaaagg ataataaaat agggatacgc ttttttctca
    10921 ataacaaaaa aacataatgg tatgaaaaaa atcaatttgc tttttatcgt gttgtctttg
    10981 gttgcagttg taaatgctca aaataagcct gcaacgccgc aattaggcaa agacagctta
    11041 aaagaagtca tcaaagccat gactttggaa gacaaaattt atatggtcat gggtctgggt
    11101 gaagaatcat ggaaaaatcc cgcctcaggg cctaaaaccg tattgcttgc cggtgctgcc
    11161 ggtcgtacat gggacattcc tcgattggga attacacctg ctgttctcac tgatggccct
    11221 gccggtttac gaattgaacc aaagcaggaa ggcttttctc atccttttta ttgtacggca
    11281 ttcccaacgg caacagtgct ggcttctact tggaatactc cactggtgga agaagtgggg
    11341 aaagctatgg gtaacgaagt tttggaatat ggttcggatg tattattggc tccggcattg
    11401 aacattcagc gaaatccgct ttgtgggcga aattttgagt attattccga agatccgctc
    11461 attgccggga aaatggctgt tgcaatggtt gttggattgc aatccaatgg cgtcggcact
    11521 tccgttaagc attttgctgc caacaatata gaaaccaaac gcaccacaat taatgcaatt
    11581 gtcagtcagc gtgcactacg agaaatttat ctgagaggtt ttgaaattgc cgtaaaagaa
    11641 ggaaatcctt ggacggtaat gtcttcctac aaccgcttga atggatttta tacttctcaa
    11701 aatcccgatt tgctgactac cattttacgt aaagaatggg gattcaaagg aatggtaatg
    11761 tccgattggt ggggtggcga tgacgcggta gctcaaatga aagcaggaaa cgacttgttg
    11821 gaacctggag ctacacagcg agaagtactc agggaagcac tgaaaaacaa aactttaagc
    11881 gaaaaaacgc ttgacagaaa tttgatgcga attctggact ttattcagaa aactcctcat
    11941 ttcaaaggtt atcaaccgtc ctatcatccc gatttggaag ctcatgccgc tattgcccgc
    12001 aaggcagcag ctgaaggtat ggttttattg aaaaacgaaa attccacttt gccattcaaa
    12061 aaagtaaata aaatagcttt gtttggaaaa acttcctatc aatttatgac tggcggaacc
    12121 ggaagtggcg aagtgaatta tgatcatgct gcagctttgg atgaggcttt tggtactgca
    12181 aaatttcaat tgtcgaaaac tttgaccgat cggtacagac aatttatcga ttcggtaaga
    12241 caacatgaga agaaagaaaa tcaaaaattt gctgttgatt ttgcaccgga attaaattta
    12301 accaaagact ttattcaatc gcaggtaaaa aattccgata ttgcggttat tactattgga
    12361 cgtcagggcg gtgaaggttg ggatcgtccc gaatcgactt tttcattgag cgaagttgag
    12421 aaaaatctga taaaaaatgt ctgtacctct ttccatgctg ccaaaaagaa agttgttgtg
    12481 gtgctcaata ttggaggcgt tatcgaaact gccgattgga gagattggcc tgatgctgtt
    12541 ttgcttgcat ggcaacccgg gcaacaaggt gctcatgcgg tggttgattt gttgaaaggt
    12601 acagaaaatc cttccggcaa actggctgtc agctttccga tgaaatatgc tgatgttcct
    12661 tcggctgcaa cttttcccgg cgaacccaaa gataaccccg tcaatgcttt ttatacagaa
    12721 ggtatatatg tcggttatcg gtattatgaa actttcaaag tgccgacagc ttatcctttt
    12781 ggttatggac tttcctatac cagtttcaaa tattccgatt tgaagttgag caatactttg
    12841 tttgataacg aaataagcgt ttcggtaaaa attaccaata tcggaaaaac agccggcaaa
    12901 gaagttgttc aactttaccg ggaagctccc aaacaagcca tcgacaaacc tgtaaaggaa
    12961 ctgaaagctt ttgccaaaac tcaattgcta aaacccaacg agagtgaagt gataactttt
    13021 aagctggatg cccgttcttt ggcttctttc tggagtggtg tcagtgcttg gattgctgac
    13081 gaaggcaaat acaaaataca tgtaggagcc tcatcggaag atattcgcgc taccggtgaa
    13141 ttttctttgc caaaaaaaat aatggtggaa aaaacacatg atgttcttta tcctaattta
    13201 ttactgcatg aaatatctcc ggaaaaatga gatgagaaaa tttatttttg tttcaatatc
    13261 gttataattt gaaatttaaa actatttttg tcatagattt ttttagttgt tttttcaaat
    13321 atctttttta attcttactc tgtgatacag ttagaagcac tttataaaat atcttatggg
    13381 ttatatattg taacttctgg agatagaaac cagggaaatg gttttgtttc aaatacggta
    13441 tttcaagtta cttctcagcc tcagcgtttt gccgtttgct gtaacaagga taattattcc
    13501 gcttcgttta ttcagcagtt caaggctttt gccgtttcca tactggcaca ggatacttcc
    13561 gccgaactta ttggcaagtt tggatataaa agcggaaggg agatgaataa aatgacggac
    13621 gtaaaagtca tttttaagga aaccaatgtc cccattgtac tcgatagcag ccttgctttt
    13681 tttgaatgta agctggttga tgtttttgat gtgggaacac attggatttt tattggcgaa
    13741 ctggttagtg ccgaaattgt cgacgatacc ggtgaacctt tgacttatgc gtattatcga
    13801 gaggtaaaaa aagggttttc acccaaaaat gctccaacgt atatagatca atcaaaaata
    13861 gaaactaaaa ctgaaaatgc tatgtcgaaa aaattcaaat gtccaatttg cggctacatt
    13921 tacgatgagg ccgaagaaaa agtaaaattt tccgatctcc ccgatgattg ggtttgcccg
    13981 gtttgtggtt cagaaaaatc ggagtttata gaaatttctt aaataaatca ttcagtaact
    14041 cattaaaaaa acaaaattat ggataaatcg ttaaaaggga caagaacaga acaaaacttg
    14101 ctgaaagcct ttgccggcga atcgcaagca cgtaaccggt acgaattttt tgccagcgtt
    14161 gccaagaagg aaggttacga acaaattgct gccatcttta tggaaactgc cagtcaggaa
    14221 aaggaacacg ccaaaagatt cttcaaattt ctggaaggtg gcatggtaga aatcaccgct
    14281 tcctatcctg caggaattat cggaacaacc aaacaaaatc tgaaagctgc agctgaagga
    14341 gagtatgaag aatggtcaag tttatatcca acatttgctg aggttgccaa agaagaagga
    14401 tttcctgaaa tttccgtagc attcaaaatg gtggctaaag tagaagtgga acacgaacgt
    14461 cgttatctga aacttttaca gaatatttcc gaagacaagg ttttcaaaaa agacggcaaa
    14521 gtgctgtggc aatgcagaaa ttgtggctac atttacgaat cggaagaagc gctggaagtt
    14581 tgtccggttt gcttgcaccc caaagccttc atgcaactga aagaagacaa ttattgaaaa
    14641 actttctcga aaaaattgaa ctctaattga gcaggtaggg ggattactcc cccgccctac
    14701 tcgattta
//
LOCUS       DAOTAX010000051        14555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3495_length_14555_cov_29.489310, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000051 DAOTAX010000000
VERSION     DAOTAX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3495_length_14555_cov_29.489310"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..230)
                     /locus_tag="PLM04_06260"
     tRNA            complement(156..230)
                     /locus_tag="PLM04_06260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Pro,seq:tgg)
     gene            351..1478
                     /gene="hemW"
                     /locus_tag="PLM04_06265"
     CDS             351..1478
                     /gene="hemW"
                     /locus_tag="PLM04_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HON02714.1"
                     /translation="MAGIYIHIPFCIQRCSYCNFFSVVSINLVNEVINAIIKELSIRK
                     DYLKNEPVQTIYFGGGTPSLLTIQQIEKLLNTVYTNFPVEQNAEITFEANPDDLTKNY
                     LFDLKKVGINRLSIGIQSFDDDKLKILNRRHNATQAHEAVREAQKAGFSNISIDLIYG
                     LPFQSLDEWETDLDKAFSLSIQHLSAYGLTFEEKTLLSKQKEKKIIEPADEELMIKMY
                     ELLLEESSKKGFDAYEISNFSLPGFYSRHNSSYWNQTPYLGVGPSAHSYNGISRQWNV
                     AAIQKYTEGILKNSSFYEQEILSLEDRYNEYVLLGLRTKNGINIDALEQNFGTELKDF
                     CLENIKTFIDNEKAYRCGSFLRLNTKGKLISDTIIAQLMKV"
     gene            1530..3848
                     /locus_tag="PLM04_06270"
     CDS             1530..3848
                     /locus_tag="PLM04_06270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HON02715.1"
                     /translation="MENQNNDKRTKFKKIFWNLFGFGILCLVLLFAFISFGWIGYLPP
                     LEELQNPQNKYASEIYSSDMQLLGRFFKNKENRVEVHFNDISKNVINALIATEDKRFY
                     KHSGIDGKALARAILLTGILQQKSAGGGSTITQQLAKQLYSPQADNIFERMLQKPIEW
                     VISVKLERNYTKEEILTMYLNQFDFLYNAVGIKSAAQIYFSTSPSQLKIEEAATLIGM
                     CKNPAYFNPVRYNERTRDRRNMVLYQMYKCDYLTKQQYDSLKNLPLVLHFQRIDHKEG
                     LAPYFREYLRTTLMAKEPHRSDYASWQDQQYLDDRWAWDNDPLYGFCNKNLKSDGTPY
                     NIYTDGLKIYTTIDSRMQRYAEEAVNEHIQTLQAAFFKEKKNRSYAPFSSSLSPKEIE
                     DILNRSIRQSERYSKMKAQGKSDAEIMEEFKKPLEMRVFSYDGDIDTIMSPLDSIRYH
                     KHFLRCGFMSMEGRSGFVRAYVGGPNFNRFQYDMVTKGRRQVGSTIKPFLYTLAMEEG
                     MWPCDQTVNQPITFRTATGELWTPRNSSKARIGETVTLRWGLANSNNWISAYLMSLFT
                     PESLVKLMRSFGIRGHIDPVVSLFLGPCEISVEEMVDAYTAFMNKGIRVEPVYVTHIE
                     DANGNVIGKFSPKMHEIFSETTAYKMIYMLQGVIDGGTGSRIRTRFGLTMPMGGKTGT
                     TQNNSDGWFIGFTPSLASGVWVGGEERSIHFDRMSEGQGASMALPIWALYMKKVLADS
                     TLNYSPRENFEIPSWFNPNAGCEDSSNGNSNE"
     gene            3946..5895
                     /locus_tag="PLM04_06275"
     CDS             3946..5895
                     /locus_tag="PLM04_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HON02716.1"
                     /translation="MDIGKNAFYFEDYVLSIQYFNQVIKIKPYLSEPYIYRAMAKIQL
                     GDYQGADEDCTQAIEKNPFVPQAYYARGFARLKMNFCNEAIDDFSKALEFSPGNIYLL
                     GYRLMARDQCSDYAGALNDVNEYLKLSPKSTEMYYEKGRILLALKDTVGAEQCFDKYI
                     QLNENSSLGWSARALLKMQKKDKKGALKDYDEAIKRNSTYSGDYINRGVLNTENKNFR
                     QALEDYDKAISLDKNNELAYFNRGLLRASLGDKNNALADFAKVLELNPSNTDALWQKA
                     TVENALGNYSQSIKDFSKIIEQYPYFIPAYSGIAEAYQKLGDEKNAFRYRQMAYNIEQ
                     NKNNIRKEELAAGNKIAKETQKNVSEKNLDFFNRFAVQDKNDTAGESKYNNNIRGAIQ
                     NRYADVVNEKNFVLTYYSKADEIRRTNLFHLSIERYNSQKKLPLPLKITNNELPLTAE
                     MISQHFDAINNISAQLEQNSSDADIYFARAIEFALVQDFKSAIDDLNKAISLKPDFMP
                     AYFTRANIRYKMIETNSNGNLGGSANKPVNDIGSNNQLKFDMELILRDYDKVIELVPD
                     FTFAYYNKANVLTAIRDFRSAVSYYSKAIDIDPDFAEAYFNRGLTYLFIGDDKNGLSD
                     LSKAGELGIYKSYNLIQRFKQQKEN"
     gene            complement(5892..6818)
                     /locus_tag="PLM04_06280"
     CDS             complement(5892..6818)
                     /locus_tag="PLM04_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HON02717.1"
                     /translation="MTLQQLEYILAVYKTGHFMKAAELCQVTQPTLSAMIQKLEEELD
                     CKIFDRSQHPIQPTEMGEKIIAQAQIILYQTNQLKEMLRKEKETVSGNLNLAIIPTIA
                     SYLLPRFIALFRNDYPEVSLKITEMRTDTIIQKLKTAEIDMAILATPLNNPDLLEIPV
                     YYEKFIAYISPGEPIYELEELSANNLPLEHLWVLEEGHCFRSQIFNFCDQMKVHNYMY
                     EAGSIETLVKIVDINGGYTVIPELHVESLTEEQKKNLRNIVQPEATREISIVIRNDYV
                     REGLLNAVADSLKKIIPPHMLDSRLKKFAIKI"
     gene            complement(7000..7842)
                     /locus_tag="PLM04_06285"
     CDS             complement(7000..7842)
                     /locus_tag="PLM04_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HON02718.1"
                     /translation="MDNITETVIKPKSSLLEIDFKELWRNRDLFSMFVKRNIVTQYKQ
                     TILGPAWFFIQPALTTIMYMVVFGGIAGIPTDGLPQPMFYLAGIVCWQYFADCLTKTS
                     STFIDNQNIFGKVYFPRLIVPLSTVASNLVRMFIQFLLFVAVYVFYLIKGVHVEPNIY
                     ILLVPVLILMLAGLSLGFGVIISSLTTKYRDLTILFNFIVQLWMYATPIIYPLSTMPP
                     DRQWIMALNPLTSIIEAFKYGTMGVGTFSWGQLAYSFGFMVVLLAVGIVIFNKVQRTF
                     MDTI"
     gene            complement(7888..9222)
                     /locus_tag="PLM04_06290"
     CDS             complement(7888..9222)
                     /locus_tag="PLM04_06290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HON02719.1"
                     /translation="MEIAIVGTGYVGLVTGTCFAEMGIDVTCVDIDEQKIEKLKSGII
                     PIYEPGLGDMVLHNVKAGRLHFTTKLTDVLDDVQVIFSAVGTPPGEDGSADLRYVLDV
                     ARTIGQNMNQYVLLVTKSTVPVGTAKLVRQTIQNELDKRGVSIEFDVASNPEFLKEGD
                     AIKDFMSPDRVIVGVESEKAREIFARLYRPFLINNFRVIFMDIPSAEMTKYAANSMLA
                     TRISFMNDIANLCEIVGADVNMVRKGIGTDQRIGSKFLYAGCGYGGSCFPKDIKALIQ
                     TAREKGYEMKVLKAVEEVNNTQKEILYRKLIKHLGENLEGKTIAIWGLSFKPNTDDVR
                     EAPALLLTEKLAKAGCHVRVYDPVAMPEAKTALSKILPEFLDKIHFASDIYDAALESE
                     ALLLVTEWKEFRMPSWGVIRKAMKNPLILDGRNIYDKKELQELGFTYEGIGV"
     gene            complement(9262..>9480)
                     /locus_tag="PLM04_06295"
     CDS             complement(9262..>9480)
                     /locus_tag="PLM04_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HON02720.1"
                     /translation="NIGCGEDIRIKDLAEMIKKIVGYEGKIVWDASKPDGTPRKLLDV
                     SKLNALGWNPKITLEDGIRQVVNNYVFS"
     assembly_gap    9481..9580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9581..10137)
                     /locus_tag="PLM04_06300"
     CDS             complement(<9581..10137)
                     /locus_tag="PLM04_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HON02721.1"
                     /translation="MDKNSKIYIAGHKGMVGSAIMRNLQNKGFSNIVGRTREELDLLN
                     QISVARFFDEEQPEYVVLAAAKVGGIVANNTLRAQFIYENLMIQNHVIHQSYLHGIKK
                     LLFLGSSCIYPRLAKQPIKEEYLLTGELEPTNEPYAIAKIAGIKMCEAYHAQYGCNFI
                     SMMPTNLYGPNDNYDLEKSHVLPAL"
     gene            complement(10152..11306)
                     /gene="gmd"
                     /locus_tag="PLM04_06305"
     CDS             complement(10152..11306)
                     /gene="gmd"
                     /locus_tag="PLM04_06305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HON02722.1"
                     /translation="MENKKVALITGITGQDGAYLADFLLKKGYLVHGIKRRSSLFNTD
                     RIDHIYQDPHEQNRNFVLHYGDMTDSLNLTRIIQETQPDEIYNLAAMSHVKVSFDTPE
                     YTANADGIGTLRLLEAIRLLGLTEKTKFYQASTSELYGLVQETPQTEKTPFYPRSPYA
                     VAKLYGYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAASRIALGLQKNL
                     FLGNLSSKRDWGHARDYVKAMYLILQQDQPDDYVIATGKTTEVREFVRLAFEQMGLTI
                     AFEGKDLNEKGYIVQIDEPLFVQKIGEEFLSLVKSLKHPVIEVDPTYFRPTEVDLLMG
                     DATKARTKLNWQPEYDLEALIKDMMQSDIKLMKKELHLKNGGFKVLNYFE"
     gene            complement(11346..13718)
                     /locus_tag="PLM04_06310"
     CDS             complement(11346..13718)
                     /locus_tag="PLM04_06310"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HON02723.1"
                     /translation="MNSETDNINQNFTEEESSFDFQKWLRLFIKNWYLFLIFIPAFLI
                     GGYLKNRSWMPIYQSVGTIIINENKYRSATQNFMQGFGLEDAYQSVNNQVIILNSYDL
                     IGKVVDSLPFMKVDYLTVGRFKTRNLYKTTPIIIETDYVAPEAYELLYKISLHPDGTF
                     TINPQNERNIPGSPINGRLGVPLQHNLFFMTVKGVNNYREFEMYFRFRTRESLIADFK
                     SRLSSKYVIEGSSVLEISLSSETPERDVDFINKLSETYLEENLKKKNDVASKTIQFIN
                     EQLSEVSKSLSVSEDEMTRFRKDNQIIDISSHGGELMSKANSLDEKKAELKLKETYLN
                     YLTKYLTGNFEEGTVVAPSNLGLNEPMLMQLVQQINDLYLQRSELTEKNLYYAKYTKA
                     IENVKISIFELIKNMRASLEIEKNEVNRLLAELNAQIAELPKKELEMIGIEKNYKVND
                     NYYTFFLQKRAEAEIQKASNTPDNNILDKARTLAVINEDEKSNNMLVFILLGFLIPTL
                     IIVLKEVSNNTIRSIKDIEKRSVFPVIGMIRHAKNDLPDLTLKNPKSSFTEMFRVIRT
                     RVEFVVRRKTDIVMMVTSVDAGDGKTFFSTNMAGVYGMTGHKTLLIDMDIRKPAINRR
                     FNVNHPKGVTNYLINECTLDEIINVVPEVNYDVIFAGTVPPNPAELIRSEKIIEMMGE
                     LRKRYDYIILDSSPIGLVTDSFSLASLADVNLLVVRNNKTHKDFFKNVVNQIVTDKLS
                     NFYVVFTDVETDGNYYSYKYGYSSKYGYYTSKGQKNIDEYRKYYDDNNEI"
     gene            complement(13753..14499)
                     /locus_tag="PLM04_06315"
     CDS             complement(13753..14499)
                     /locus_tag="PLM04_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HON02724.1"
                     /translation="MKHLLHIIFTVLVVLSFSSCYNYKSIGLLQENKSLPVYAKEDYS
                     DYKICVNDEIIFRLITTDETISKLISSNQSGNSSQMISYRVYPDGTVDIPFLNRVQVK
                     GLTTNEAENLLKKEFREIIPDAEVKLSIANKSFTVIGEAGTGVFPVYKDKLTIYQALA
                     MSGDLKLSGDFRHVKILRAEEGKTEILEFDIRPASVIESKYYYVYPNDIIYVQRASSS
                     FFKVNNYSSFLGLISSSLSLLFSVLYYIKL"
BASE COUNT         4603 a         2868 c         2575 g         4409 t
ORIGIN      
        1 ccctccagcc tttagctgga tgcgctaacc ttgtaaattt tttaaatatc gttcttctat
       61 tgtgtcgggg tagcgggatt tgaacccacg accctccagc ctttagctgg atgcgctaac
      121 cttgtaaatt ttttaaatat cgttcttcta ttgtgtcggg gtagcgggat ttgaacccac
      181 gaccccctgc tcccaaagca ggtgcgctaa ccggactgcg ctacaccccg tactgatttt
      241 tttaaaaacg actgcaaagt taaaatcttt tgccgaaaat accaactttt tgctgtattt
      301 ttgtataaaa gaattcttga attgttccta atttctttcc cgctttaaaa atggccggta
      361 tttacattca tattcctttt tgcatccagc gatgttccta ttgcaatttt ttttccgtag
      421 tctctatcaa tcttgttaat gaagttataa atgcaataat taaagaattg tcgatcagaa
      481 aagactattt gaaaaatgaa ccggtacaga cgatttattt tggcggagga acacccagtc
      541 tgttgacgat tcaacaaatt gaaaaattac tgaatactgt ttatacaaat ttcccggtag
      601 aacaaaacgc agaaataacg tttgaagcca atcctgatga tttaacaaaa aattatttgt
      661 tcgacctgaa aaaagtaggc atcaatcggt taagtattgg aattcaatca tttgatgacg
      721 ataaacttaa aatactcaat agaagacaca atgcgacaca ggctcatgaa gcagttcgtg
      781 aagctcaaaa ggcaggtttt agcaatatca gcatagattt aatatatgga ttacccttcc
      841 aaagcctcga tgaatgggaa actgatttgg ataaagcttt ttcactgtcc attcaacatc
      901 tttctgccta tggcctaacc tttgaagaaa aaacactttt atcgaaacaa aaagaaaaaa
      961 aaatcataga accggcagac gaagaattga tgataaaaat gtatgaactt ttgttggaag
     1021 aaagcagcaa aaaggggttt gatgcttacg aaatatccaa tttttcctta ccgggatttt
     1081 attcacgaca taattcttcc tattggaatc aaactccata tttgggcgta ggaccttccg
     1141 cacattccta taatggcatt tctcggcagt ggaatgttgc tgctattcag aaatatacag
     1201 aaggaatttt gaaaaacagt tctttttacg aacaggaaat tctcagtttg gaagatcgtt
     1261 ataatgaata tgtattactc ggattgagaa caaaaaacgg tataaatatt gatgcgttgg
     1321 aacaaaattt tggtaccgaa ttgaaagact tttgtttaga aaacataaaa acatttatcg
     1381 ataatgaaaa ggcatatagg tgcggcagtt ttttacgttt aaacacaaaa ggaaaactaa
     1441 tatccgacac cattatcgct caattaatga aggtttaagt tatggaaaat tattactaat
     1501 tttgtaactt attcgttaga ttaaaaaata tggagaatca gaacaacgac aagcgtacta
     1561 aatttaaaaa aattttctgg aatttatttg gatttggcat cctttgcttg gttttgctct
     1621 ttgctttcat tagttttggc tggattggtt acttgcctcc actcgaagaa ttacaaaatc
     1681 ctcaaaacaa atatgcttcg gaaatttact cttcagacat gcaattgctg ggtagatttt
     1741 ttaaaaacaa ggaaaatcgt gtagaagtac atttcaacga catttctaaa aatgttatta
     1801 atgctttgat tgctactgaa gacaaaagat tttacaaaca ttccggcatc gacggcaaag
     1861 cgcttgcccg agctatttta cttaccggta ttttgcagca aaaaagcgcc ggaggaggaa
     1921 gcaccattac ccaacaattg gccaagcaac tttattcacc tcaagccgac aacatttttg
     1981 aacgaatgct tcaaaaaccc attgaatggg taatatctgt caaactggaa cggaactaca
     2041 caaaagaaga aattctgacc atgtacctta atcagtttga tttcctttac aatgctgtag
     2101 gaataaaatc ggctgctcaa atctatttca gcaccagtcc ttctcaactg aaaattgaag
     2161 aagctgccac tttgatagga atgtgcaaaa atcccgccta ctttaatccc gtacgttaca
     2221 atgaacgtac acgcgacagg cgcaatatgg ttttatatca aatgtacaaa tgtgattatc
     2281 tcacaaaaca acagtatgat tcattaaaaa atcttccgtt ggtgcttcat tttcagcgaa
     2341 tcgatcacaa agaaggattg gccccctatt tcagagaata cctcagaacc acattaatgg
     2401 caaaagaacc tcatcgaagc gattatgcca gctggcaaga ccaacaatat ctcgatgatc
     2461 gctgggcttg ggacaatgac cctttgtatg gattctgcaa taaaaatctg aaatcagacg
     2521 gaacgccgta caatatctat actgatggtt tgaaaattta taccaccatc gattctcgta
     2581 tgcagcgata tgcagaagag gctgtaaatg aacacattca aacattgcag gctgcatttt
     2641 tcaaagaaaa gaaaaacaga agttatgctc ctttctccag tagcctgagc cccaaagaaa
     2701 tagaagatat tttgaaccgc tcgatccggc aatcggaaag atattctaaa atgaaagcac
     2761 aaggcaaaag cgatgccgaa attatggaag aattcaagaa acctttagaa atgagagttt
     2821 tctcttatga tggtgatatc gacaccatta tgtcgccact cgattccatt cgctaccaca
     2881 aacatttctt gcgttgtggt ttcatgtcga tggaaggacg cagcggattt gtcagagctt
     2941 atgttggtgg cccgaatttc aatcggttcc aatacgatat ggtcaccaaa ggacgccgac
     3001 aagtgggttc aaccatcaaa ccgttccttt acacattggc aatggaagaa ggaatgtggc
     3061 cttgcgatca aacagttaat cagcccatta ctttcagaac agccaccggt gaactttgga
     3121 caccaagaaa ctcttcaaaa gccagaattg gagaaacagt taccctgcgt tggggacttg
     3181 ccaattcaaa caactggatt tcagcttatt taatgagtct tttcactccc gaatcgcttg
     3241 taaaacttat gcggtccttt ggcattagag gtcatattga tcccgttgta tcattgtttt
     3301 tgggaccttg cgaaatttcc gttgaagaaa tggtggatgc ctatactgca tttatgaata
     3361 aaggaattag agttgaaccg gtttatgtaa ctcacattga agatgccaac ggaaatgtaa
     3421 ttggaaaatt ttctccgaaa atgcacgaaa ttttcagtga aactacggct tacaaaatga
     3481 tttacatgct acagggagtt attgacggag gaaccggttc gagaatcaga acacgtttcg
     3541 gattaaccat gccgatggga ggaaaaacgg gaacgacaca aaacaattcg gacggttggt
     3601 tcatcggatt tactccttca ctggcttcgg gggtttgggt tggtggtgaa gaacgttcca
     3661 ttcatttcga cagaatgtcg gaagggcaag gtgcaagtat ggctttgcca atttgggctt
     3721 tgtacatgaa aaaagttttg gctgattcaa ctttaaacta ttcacccaga gaaaattttg
     3781 aaattccttc ctggttcaat cccaatgccg gttgtgaaga tagcagtaat ggaaatagta
     3841 atgaataata atagattcgt ccacttcaga aaaaaatttc ttctgtgctt cacaggatta
     3901 ttattttcct ttgccgccca tgctcaatgg aataccgata gaattatgga catagggaaa
     3961 aatgcttttt attttgaaga ttatgtactt tccattcagt atttcaatca ggtaatcaaa
     4021 attaagccgt atttgtcgga accttatatt tatcgggcaa tggcaaaaat tcaactcggc
     4081 gattatcagg gcgccgacga agactgtacg caagctattg aaaaaaatcc ttttgttccg
     4141 caagcctact atgccagagg tttcgcccga ttgaaaatga atttttgtaa cgaagctatc
     4201 gatgatttct cgaaagcgct ggaatttagt ccaggaaata tatatttgct tggctatcgg
     4261 cttatggcac gcgaccaatg tagtgattac gcaggtgctt tgaatgatgt aaacgagtat
     4321 ctgaaattaa gccccaaaag tacggaaatg tattatgaaa agggcagaat tctgttggca
     4381 ttaaaagaca cggttggagc cgagcaatgt ttcgacaaat acattcagtt gaacgaaaat
     4441 agcagcttgg ggtggagtgc tcgtgcttta ttgaaaatgc agaaaaaaga caaaaaagga
     4501 gctttgaaag attatgatga agccatcaag agaaattcaa cctacagcgg agattacatt
     4561 aacagaggcg tattaaacac ggaaaacaaa aatttcaggc aagctctgga agattacgat
     4621 aaagcaattt cactggacaa aaacaatgaa ttggcttatt tcaacagagg tttactcaga
     4681 gccagtcttg gagataaaaa taatgcactt gcagactttg caaaagtttt ggaactcaat
     4741 ccatccaata ccgacgctct ctggcaaaaa gctacagtag aaaatgcact gggaaattac
     4801 tcgcagtcca taaaagattt ttcaaaaatt atcgaacaat atccttattt tatcccggcc
     4861 tactctggaa tagccgaagc ctatcagaaa cttggtgatg agaaaaatgc cttcagatac
     4921 aggcaaatgg cttataacat agaacaaaac aaaaataaca tcaggaagga agaattggct
     4981 gccggcaata aaattgcaaa agaaacccag aaaaatgttt ctgaaaagaa tctcgatttc
     5041 ttcaatcgtt ttgctgtgca ggataaaaat gatactgccg gcgaatcaaa atataacaac
     5101 aacattcgtg gtgcaataca aaaccgatat gctgacgttg tgaacgaaaa gaattttgtg
     5161 ctgacttatt acagtaaagc tgatgaaatt cgcagaacca atcttttcca tctgtccatt
     5221 gaacgctata acagccagaa aaaactgccg ctgcccttaa aaattaccaa taacgaatta
     5281 cccctgacag ccgagatgat tagtcagcat ttcgatgcta tcaacaatat ttctgcgcaa
     5341 cttgaacaaa actcatcgga tgcagatatt tattttgcca gagccattga atttgctctg
     5401 gtgcaggatt ttaaaagcgc tattgatgat ttgaacaaag ccatttcgct caaacccgac
     5461 tttatgccgg cttattttac ccgagcaaat attcgataca aaatgattga aacaaattcc
     5521 aatggaaatt tgggtggttc tgccaacaaa ccggttaacg acattggttc gaataatcaa
     5581 ttgaaatttg acatggaatt aattctgaga gattatgata aggtcataga attggttccc
     5641 gatttcacgt ttgcttatta caacaaagcc aatgttttaa ctgccattcg cgattttcgt
     5701 tcggctgtaa gttactacag caaagccata gatattgatc ccgactttgc agaagcctat
     5761 tttaatcggg gattaaccta tctgtttatc ggagacgaca aaaacggact gtcggatttg
     5821 agcaaagccg gagaattggg aatttataaa agctataatc tgattcaaag attcaagcag
     5881 cagaaagaaa actaaatttt aatggcaaat tttttcaagc ggctatccag catgtgagga
     5941 ggtattattt tcttcaggct gtcagcaacg gcattcaaca agccttcgcg aacataatcg
     6001 tttctaatga cgatggaaat ttctctggtg gcttcgggtt gaacgatatt tcgaagattc
     6061 tttttctgct cttcagtaag tgactcaaca tgcaattcgg gaattaccgt ataacctccg
     6121 ttgatatcca caattttcac caatgtttca atactgccgg cttcgtacat gtaattatga
     6181 actttcattt ggtcgcagaa attgaaaatc tgacttctga aacaatgacc ttcttccagc
     6241 acccaaagat gctccaacgg caaattgttg gccgacaatt cttctaactc ataaattggt
     6301 tcgccgggag aaatataggc aataaatttt tcgtaataaa caggaatttc gagcaagtca
     6361 ggattgttaa gtggcgttgc caatatcgcc atatcaattt cggcagtttt caacttctga
     6421 ataatggtgt cggtacgcat ttcagttatt ttcaacgaga cttccggata atcgtttctg
     6481 aacaaggcaa taaaacgcgg caaaagatag gaagcaatag ttggaataat tgccagattc
     6541 aaatttcccg aaacagtttc cttttctttc cgtaacattt ctttcaactg attggtctga
     6601 taaagaataa tttgagcctg agcaataatt ttttctccca tttcggtggg ctgaatggga
     6661 tgctgcgagc ggtcaaatat cttgcagtca agttcttctt ccaatttttg aatcatggcg
     6721 ctcaaagttg gctgagttac ctgacacaat tcggcagctt tcataaaatg acccgtttta
     6781 tatactgcca gaatatattc caattgctgt aaagtcatgt agaaaaatta ttaaattaac
     6841 gtgagttcga tataaagacc ttatctaaac aaaactaatc tcagtaatag gagaattttc
     6901 cccttttttc atcttcctta ttaactaatt tttaattact tataaaacat tcttatacct
     6961 aactcaattt taaccaatta aattagcttt gatttatttc taaatagtgt ccatgaatgt
     7021 tcgctgcact ttgttgaaaa taacaattcc aaccgccaga agaacaacca taaaaccaaa
     7081 actgtaagcc aattgccccc agctgaaagt tcctacgccc atagttccgt acttgaaagc
     7141 ttcaataatg gatgtcagcg gattcaatgc cataatccat tgacgatcgg gcggcatggt
     7201 gcttaaagga taaataattg gcgtagcata catccacaac tgtacaataa aattgaacaa
     7261 aatagttaaa tcgcgatact tggtggtaag tgaagaaata attacgccga agccaagtga
     7321 taagccggcc agcataagaa tcagtacagg aacgagcaaa atataaatat tcggctcaac
     7381 atgaacgcct ttaatcaaat aaaaaacata tacggcaaca aaaagcaaaa actgaataaa
     7441 catccgcaca aggttggaag cgacggtaga aagtggaaca atcagtcgtg gaaaataaac
     7501 ttttccgaaa atattctgat tgtcgataaa agtactggaa gttttggtca aacaatcggc
     7561 aaaatactgc cagcaaacaa tgccggccaa ataaaacatg ggctgtggta atccatcggt
     7621 cgggatgccg gctataccgc caaaaacaac catgtacatg atggttgtca atgccggttg
     7681 aatgaaaaac cacgcagggc ccaaaatggt ttgcttgtat tgcgttacga tattgcgctt
     7741 aacaaacatg gaaaataaat ccctatttct ccataattcc ttaaaatcaa tttccaacaa
     7801 actgcttttg ggcttgataa ctgtttcggt aatattatcc atacaatatt ctctcagtaa
     7861 aatcaacaaa ttatgtatat tttgtcatca aactccaata ccttcataag taaatcccaa
     7921 ttcttgaagt tcttttttgt cgtaaatatt tcgtccatca agaatcaaag gatttttcat
     7981 tgctttcctg ataacgcccc aactcggcat tctgaattct ttccattcgg taaccagcaa
     8041 cagagcttcc gattctaaag cagcatcgta aatatcggaa gcaaaatgaa ttttgtcaag
     8101 aaattcaggc aaaattttgc tcaacgctgt ttttgcttcg ggcatggcca ccggatcgta
     8161 aacacgaaca tggcatccgg cttttgccaa tttctctgtc aacaacaacg caggagcttc
     8221 acgaacatca tctgtattgg gcttaaatga taatccccaa atggcaatgg ttttcccttc
     8281 caaattttca cccaaatgct taatcaattt acggtaaaga atttctttct gagtattatt
     8341 tacttcttct acagctttca atactttcat ttcgtatcct ttttcccttg cagtctgtat
     8401 aagagcttta atgtcttttg gaaagcagga accaccgtaa ccacagccgg catacaggaa
     8461 tttactgcca atgcgctgat cggtaccaat accttttctt accatattaa catcggcacc
     8521 cacaatttcg caaaggttgg caatatcgtt cataaaactg atacgtgttg ccagcattga
     8581 attagctgca tatttagtca tttcggcgga aggaatatcc ataaaaataa cacgaaaatt
     8641 gttgatcagg aaaggacggt acaagcgggc aaaaatctcg cgtgctttct cgctttccac
     8701 acccacaata actctgtcgg gactcatgaa atcttttatg gcatcacctt ctttcagaaa
     8761 ttcgggattg gaagccacat caaattcaat tgacaccccg cgtttgtcca attcattctg
     8821 gatggtttgg cgaacaagtt ttgctgttcc tacgggaacc gtacttttgg ttaccagcaa
     8881 tacatattga ttcatgtttt gccctatcgt gcgtgccaca tcaagaacat accttaaatc
     8941 ggcacttcca tcttcaccgg gaggtgttcc tacagcactg aaaattacct gaacatcgtc
     9001 cagcacgtcg gttagttttg ttgtaaaatg caaacgacca gctttgacat tgtgtaaaac
     9061 catatctccc aatcccggtt cataaattgg aattatgccg cttttcaatt tttctatttt
     9121 ttgttcatca atatccacac acgtaacatc gattcccatt tcggcaaaac aagtaccggt
     9181 aaccaaaccg acgtacccgg ttccaacaat agctatttcc atattatcaa gttaattttt
     9241 tccctattaa aaatgtccga atcatgaaaa aacataattg tttactactt gacgaattcc
     9301 atcttcaaga gtaatttttg ggttccaacc caaagcgttc agtttggaaa catccagtaa
     9361 ttttcgtgga gtaccatccg gttttgatgc atcccaaaca atttttcctt cgtaaccaac
     9421 aattttttta atcatctcag ccagatcctt tatccgaata tcttctccac aaccaatatt
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcaaagccg gtaatacgtg
     9601 cgatttctca agatcataat tatcattggg gccgtaaaga ttggtgggca tcatcgaaat
     9661 aaaattacag ccatactgag catgataagc ttcacacatt tttatgcctg caatttttgc
     9721 aatggcataa ggctcgttgg ttggctccag ctcacctgtt agcaggtatt cttccttaat
     9781 aggttgtttg gccaaacgtg gataaatgca cgaagaacca agaaacaaga gtttttttat
     9841 cccatgcagg taactttgat gaatgacatg attctgtatc atcaaatttt cataaataaa
     9901 ctgggcacgt aaggtattgt tggccacaat tccgcccact ttggcagctg ccagcacaac
     9961 atattccggt tgttcttcat caaaaaaacg ggcaaccgaa atttgattca acaaatcgag
    10021 ttcttccctg gttcgtccaa ctatattcga aaatccttta ttttgcaaat ttcgcataat
    10081 cgccgaacct accatccctt tgtgtccggc tatatatatt ttggaatttt tatccattgt
    10141 aaattttata attattcaaa ataattcagc actttaaaac cgccattttt taaatgtagc
    10201 tcctttttca ttaatttaat atccgactgc atcatgtcct tgatcaatgc ctccaaatca
    10261 tattccggtt gccagttcaa tttggttctg gctttcgttg catcacccat caacaaatcc
    10321 acttcagtcg gacggaaata ggttggatca acttctatca caggatgttt aagagatttt
    10381 actaacgaca aaaattcttc tccaattttt tgcacaaaaa gtggctcatc tatttgcaca
    10441 atgtatcctt tttcgttcaa atcttttccc tcaaatgcta tcgtcaagcc catctgttca
    10501 aaagccaatc gaacaaattc cctgacttct gtggtttttc ccgttgcaat tacataatca
    10561 tcgggttgat cctgctgtaa aatcaaatac attgctttca cataatcgcg ggcatgtccc
    10621 caatcccgtt tggaggacaa atttcccaga aaaagatttt tttgcaagcc caatgctatt
    10681 ctggatgctg cacgcgtaat ttttcgcgta acaaaagttt caccacgcaa tggcgattca
    10741 tgattgaaca aaatgccatt gctggcatga ataccataag cttctctata atttaccgta
    10801 atccagtaac cataaagttt tgccacagca tacggtgaac ggggataaaa aggagtcttt
    10861 tcggtttgtg gagtttcttg aaccaaacca tacaattcgg aagttgaagc ctgataaaat
    10921 tttgtttttt cggtaagtcc caataaacga atagcttcca gcaaacgtaa agttcctatt
    10981 ccatcggcat tggcggtgta ttccggtgta tcaaaactca cttttacatg gctcatggca
    11041 gcaagattgt agatttcatc aggttgagtt tcctgaataa ttctggtcag attcaacgaa
    11101 tcggtcatat cgccatagtg caaaacaaaa ttccggtttt gttcatgagg atcctgatat
    11161 atatgatcaa ttctgtcggt attgaacaat gaagaacgac gttttattcc atgaaccaaa
    11221 tagccttttt tcaaaagaaa atcagccaga taagcaccat cctgacccgt aatacctgta
    11281 attaatgcta cttttttgtt ttccataatg ctaatagttt gacaaataaa ttttttgtag
    11341 cttatttata tttcattatt atcatcgtag tatttgcggt attcatcaat gtttttttgt
    11401 ccttttgaag tatagtaccc atattttgac gaatatccat atttatagga atagtaattg
    11461 ccatctgttt ctacatcggt aaataccaca taaaaattgc tcaatttgtc ggttacaatc
    11521 tgattcacaa catttttgaa aaagtcttta tgtgttttat tgtttcgcac caccagcaaa
    11581 ttaacatcgg ccaacgatgc cagtgagaaa ctgtcggtaa ccaagcctat tgggctcgaa
    11641 tcgagaatga tgtaatcgta tcgtttccga agttcaccca tcatttctat aattttttca
    11701 gaccggatca attcagctgg atttggtggg actgtaccgg caaaaatcac atcgtaatta
    11761 acctcaggaa cgacgttaat aatttcatcc agcgtacatt cattaatcag ataattggta
    11821 actcctttgg gatgatttac attgaaacgc ctgtttatgg ccggttttct gatgtccata
    11881 tcaatcaata gagttttatg accggtcatt ccataaactc cggccatatt ggtcgagaaa
    11941 aatgttttac cgtcgccagc atccaccgaa gtaaccatca tgactatgtc tgttttgcga
    12001 cgcactacaa actccactcg tgtacgaatt accctgaaca tttcggtaaa ggaagatttg
    12061 ggatttttca gagtcagatc aggcaagtcg ttttttgcat gacgaatcat accaatcacc
    12121 ggaaacactg atcgcttttc aatatctttt atcgaacgaa tggtattatt cgacacttct
    12181 ttcagtacaa taatcaaagt agggataaga aaacccaaaa gtatgaaaac gagcatgttg
    12241 ttcgatttct catcttcgtt gatcaccgcc aaagtgcgag ctttgtccaa tatgttgtta
    12301 tccggagtat tggaagcttt ttgaatttca gcttctgccc ttttttgcag gaaaaaagta
    12361 taataattgt cgttgacctt gtaatttttt tcgataccta tcatttccag ttctttcttg
    12421 ggcaattcgg ctatttgagc gtttagttcg gcaagaagac gattgacttc atttttttcg
    12481 atttccaatg aagccctcat gttttttatc aattcaaaaa tggatatttt aacattttct
    12541 atagcttttg tatacttggc ataatataaa ttcttctcgg tcaactcgct acgttgcaaa
    12601 tataggtcgt tgatttgttg aactaactgc attaacatag gttcatttaa tccaagattt
    12661 gaaggagcaa ccactgttcc ttcttcaaaa ttaccggtga ggtatttggt caaataattc
    12721 aaataagtct ctttgagctt taattcggct ttcttttcat ccaacgagtt ggccttactc
    12781 atcagctctc ctccatgact cgaaatatcg attatttggt tatcttttct gaatcgtgtc
    12841 atctcatctt ctgaaactga taaagatttt gatacttcag acaactgttc attaataaat
    12901 tgaatggttt tggaggctac atcgtttttc tttttcaaat tttcttccag ataagtttcg
    12961 ctcagcttat tgataaaatc tacatctcgc tcaggtgttt cgctactcaa tgaaatctcc
    13021 agaaccgaag agccttcaat tacatacttt gaacttaatc tcgatttaaa atctgcaatt
    13081 agcgattccc ttgtacggaa acggaaatac atttcaaatt ccctatagtt gttcacacct
    13141 tttacagtca taaaaaacaa attatgctga agaggcacac ccaaacgtcc gttaattgga
    13201 gaaccgggaa tatttctttc gttttgggga ttgattgtaa aagtaccatc tggatgaagc
    13261 gaaattttat aaagcaattc ataagcttcc ggagccacat aatcggtttc aatgataatg
    13321 ggagtagttt tataaagatt tcttgtttta aaacggccga cagtcaaata gtccactttc
    13381 ataaagggca aactgtccac tactttacca atcaagtcgt atgaattaag gataatcact
    13441 tggttattga cactttgata ggcatcttcc aaaccaaatc cctgcataaa attttgtgtg
    13501 gcggaacgat atttgttttc attgataatt attgtcccaa cgctctgata gataggcatc
    13561 caacttctgt ttttcaaata tccccctata agaaaggcag gaataaatat caaaaacaaa
    13621 taccagtttt tgatgaaaag acgaagccac ttttggaaat caaaggaaga ttcttcttct
    13681 gtaaagtttt ggttaatatt gtctgtttcc gaattcatat cttagaatta aagatcgatt
    13741 tatgctgtga atttacaatt tgatataata taaaacgctg aacaataaag ataaagaaga
    13801 tgaaattaac ccaagaaacg aagaataatt gttcacttta aaaaaacttg atgaagctcg
    13861 ctgtacgtaa ataatgtcat tggggtaaac ataatagtat tttgattcta ttacacttgc
    13921 cggacgaata tcaaactcca atatttcggt ttttccttcc tctgcccgta aaattttcac
    13981 atgtctgaag tctcctgaaa gcttcaaatc acctgacatg gctaaagcct gataaatagt
    14041 aagtttatct ttataaacgg gaaaaactcc tgttcctgct tctccgatga ccgtaaacga
    14101 tttatttgca atgcttagct ttacttcagc atcaggaata atttctctga attccttctt
    14161 caacaagttt tcagcttcat tggtagttaa tccttttacc tgaacacgat ttaaaaaggg
    14221 aatatccacc gtaccatccg gataaacccg gtaagaaatc atctggctac tgtttcctga
    14281 ctgattcgaa gatataagct ttgaaatggt ttcatcggta gtaatcagac ggaaaataat
    14341 ttcatcattg acacaaattt tataatccga ataatcttcc ttagcataaa cgggaagcga
    14401 cttgttttcc tgcaaaagtc cgatactctt gtaattataa caggaagaaa aagacaaaac
    14461 aaccaaaact gtaaaaatta tatgcagtaa atgtttcata attttccctt caatcgatta
    14521 acgggccaaa ccataaataa aaattccaaa agaaa
//
LOCUS       DAOTAX010000052        13595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3815_length_13595_cov_32.474889, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000052 DAOTAX010000000
VERSION     DAOTAX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13595
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_13595_cov_32.474889"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..638
                     /locus_tag="PLM04_06320"
     CDS             213..638
                     /locus_tag="PLM04_06320"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HON02725.1"
                     /translation="MITKKRIANFKWLSMISLLMFISIQHSAQAQTSSIPNEIVSALS
                     NGDANRLSAYLNDNVELFIENKNDIYSRQQAVSILNDFFHRFKVSDFKVIHQGNRDNS
                     SFIIGILSTATENFRVYILTRKSDNKTIILQLRIETSNE"
     gene            631..1506
                     /gene="nadC"
                     /locus_tag="PLM04_06325"
     CDS             631..1506
                     /gene="nadC"
                     /locus_tag="PLM04_06325"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HON02726.1"
                     /translation="MNEDYKQLIPLWFAEDIGDGDHTTLACIPETALGKSQLIIKENG
                     VIAGVEVAREIFKTFDPTLKMNVFITDGTEVKIGDIAFNVEGKIQSLLQTERLMLNVM
                     QRMSGIATRTRQYVKALEGTKTRVLDTRKTTPGLRFLEKEAVRIGGGVNHRFGLYDMI
                     LLKDNHIDFAGGIKEAILRTKEYLKNKGKNLKIEIEVRNFDEIEQVMAIGGVDRIMLD
                     NFNIENTKKAVEIIGGRYEIESSGGITFDTLRQYAECGVDFISVGALTHSVKSLDMSF
                     KADIPNLIEGIHKIF"
     gene            1543..2091
                     /gene="rfbC"
                     /locus_tag="PLM04_06330"
     CDS             1543..2091
                     /gene="rfbC"
                     /locus_tag="PLM04_06330"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HON02727.1"
                     /translation="MEIIETPIPDLLIIRPKVFEDARGYFYETYNEKSFMEAGLDIHF
                     CQDNQSKSVYGVIRGLHYQLSPCSQCKLVSAIQGTVWDVAVDLRASSPTFGKWFGIEL
                     SAENHLQFLIPKGFAHGFSVLTETAIFAYKCDAFYNPSLERGILYNDPQLAIDWKIPE
                     DKAIVSAKDAKHPIFSKAEKNF"
     gene            2134..3003
                     /gene="rfbD"
                     /locus_tag="PLM04_06335"
     CDS             2134..3003
                     /gene="rfbD"
                     /locus_tag="PLM04_06335"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HON02728.1"
                     /translation="MQTILITGSKGQLGNELQKAAKRFPNFRYIFTDVDELDICNKSA
                     LDNFVKENRIEIIVNCAAYTAVDKAEEEVEKCFAINAQAVKNIGEIAETYQIKVVHIS
                     TDYVFDGKSYKPYTEEMEVSPDTVYGKSKLQGEKFLQSVCNQYIVIRTSWLYSSYGNN
                     FVKTMLRLGKERNSVKVIFDQIGTPTYAADLAEIILKILSQNNFISGIYHFSNEGVCS
                     WYDFATAIFKIAHVNCLVHPIETKDYPAPAPRPYYSVLNKSKIKNTFGLSIAHWEESL
                     EKCIEILKSEYSL"
     gene            3178..3900
                     /locus_tag="PLM04_06340"
     CDS             3178..3900
                     /locus_tag="PLM04_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HON02729.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAAREGGPDPDMN
                     PRLRVLFQQAKKENMPKENVERAIKKAVSKEEADNYKEVIYEGYAPHGIAVIIETATD
                     NPTRTVASIRSYFNRYGGSLGTSGSVQFLFDHKCVFRIAPKSNLSIDDLELELIDYGV
                     DEVIQDDEENNIVLYGDFQHYSSIQKYLEENNFEIFSAEFEWIPKETKELNDEQKAQV
                     LKLIEKIEDDEDVQNVFHNMAE"
     gene            4010..5158
                     /locus_tag="PLM04_06345"
     CDS             4010..5158
                     /locus_tag="PLM04_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HON02730.1"
                     /translation="MDISVLFPALASLTWQHILMILIGLTLIYLAIFKEYEPTLLLPI
                     GFGTILVNIPLSSAINQIDGKTGEIIEGILSVFYEGGIATEMFPLLIFIAIGAMIDFT
                     PLFNNPHLLLFGAAAQFGIFMTMFIATLFGFDLKEAASIGIIGAADGPTSIFVASRFA
                     PHLLGPISVAAYSYMALVPIIQPPVIKLLTSKKERLIRMQYSSKKVSRKALIAFPIVV
                     TIISGLIAPASLSLIGFLMFGNLIRECGVLNKLSQSAQNELASLVTILLGITIASTMT
                     AERFVTFGTLTIIALGLLAFVFDTFGGVIFAKLLNLILPAKKKMNPMIGACGISAFPM
                     SARVIHTMGLKEDPHNFLLMPAVSANVGGQIGSVVAGGLILTLVNLFA"
     gene            5171..5308
                     /locus_tag="PLM04_06350"
     CDS             5171..5308
                     /locus_tag="PLM04_06350"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HON02731.1"
                     /translation="MINNVEAALEIGAIGITGIFIFMLIFYAAIKLILKLFPEKENKE
                     E"
     gene            complement(5430..6248)
                     /gene="panB"
                     /locus_tag="PLM04_06355"
     CDS             complement(5430..6248)
                     /gene="panB"
                     /locus_tag="PLM04_06355"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HON02732.1"
                     /translation="MSVHNEDNRKVTTRRLFEMKQRGEKISMLTAYDYTMANIVDEAG
                     IDVILVGDSASNVICGNTTTLPITLDQMIFLAKSAIRGIKRALAVVDMPFGSYQGNSH
                     EAIASAIRIMKETHADGIKMEGGEEIIESVKRIIGAGIPIMGHLGLMPQSINKYGSYN
                     VRAQEEEEAEKLIRDAHLLEDAGCFALVLEKIPAKLGTQIASELSIPVIGIGAGGGVD
                     GQVLVIHDMLGLTQGFSPRFLRRYANLREQMNQAIQHYIADVKSSDFPNEKEQY"
     gene            6464..6928
                     /locus_tag="PLM04_06360"
     CDS             6464..6928
                     /locus_tag="PLM04_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HON02733.1"
                     /translation="MSKITIHSYQELEEYVGKELGVSDYLTITQEQINKFADATLDYQ
                     WIHVNAEKAKQESPYKNTIAHGYLNLSILPYLWGQIMEIRNSKMTLNYGIENARFQQA
                     VVVNDQVRAKAKLKSLVNLKGIAKAEIYTVLEIKNSNKPALEGTFIFLYHFE"
     gene            complement(6937..8613)
                     /locus_tag="PLM04_06365"
     CDS             complement(6937..8613)
                     /locus_tag="PLM04_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HON02734.1"
                     /translation="MEWFLSLLLNDGIAHTILLFAFVISLGILLGKIKIFGISLGVTF
                     VLFVGLFVGHLGLSVNPEVLEFIKNFGLILFVYSIGLQVGPSFFSSFKKGGMLLNLLS
                     TMIVLLGVGIVIALYYLLHGKVSMPMLVGILSGSVTNTPGLGAAQEALRHVFGSSPGA
                     EIPPIALGYAVAYPIGVLGIIFSMVLLRIIFKVDFHKETEQIKKASEVDEEQVEKISV
                     QVKNEKVFNHTVTDVKRAVGRPFIVSRLMRNGEFFIPQGDSMLYKDDILLIVASSRDI
                     ERITSYIGEKVEMDWKISEEKLVSRRIVITHGKINGKTIGSLKLRTIYGVNITRVNRS
                     GVDLLGTPDLVLQVGDRVMVVGELDAIEKVEKLLGNTLQKLNEPPIITIFVGILLGVL
                     VGSIPFFVPGIPVSVKLGLAGGPLIVAILLGRFGYKLKLITYTTTSANYMLREIGISL
                     FLASVGIGAGEKFVETVISANGLLWMGCAAAIALLPLLIVGIIGRKFLKINYFTLIGL
                     LAGSTTDPPALAYSNSIAGSDIPAVAYSTVYPLTMFLRVVSAQILILAFL"
     gene            8835..10031
                     /gene="hisB"
                     /locus_tag="PLM04_06370"
     CDS             8835..10031
                     /gene="hisB"
                     /locus_tag="PLM04_06370"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HON02735.1"
                     /translation="MVHFRNKVHYKHINKINMEENTGKTVKKRVLFIDRDGTIIVEPS
                     ETFQVDSLEQLEFLPGVIRNLYFIRQRTDFEWAMVSNQDGLGTSAYPEENFEKVQSKM
                     LKILENEGIIFDKIFIDKSLPEEKLPTRKPGTAMLKEYFSANYDLENSYVVGDRITDV
                     ELAKNLGCKAILLNEDPEILQESKLKKVCVLQTTSWDEIAAFIFAGERTATIQRTTKE
                     TDIFIHLNLDGSGKGNISTGLRFFDHMLEQIARHSGCDLTIQVKGDLEVDEHHTIEDT
                     AIALGEAFAKALGDKRGIERYGFCLPMDDSLCLVAIDFGGRAWLTFDAIFKREYIGDF
                     PTEMIFHFFKSLSDAAKMNLYVKALGENEHHKIEGIFKALAKAIKMAVKRDIYQFELP
                     TTKGII"
     gene            10099..11676
                     /locus_tag="PLM04_06375"
     CDS             10099..11676
                     /locus_tag="PLM04_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HON02736.1"
                     /translation="MIFLLIVQFPLHAQIFPDQPRLVIGIVVDGLQEKHIDLLWQRFD
                     ANGFKKIIEKGTAFENMEYNIVSAGNASDIATLMTGTTPYYHGVTGNNFYSRKQGNVQ
                     SVIFDENEPGIGTKQTVSAHQLLASTITDELMLTYPKQSKTYAIAINAEDAIMLGGHT
                     ANSAMWLDDINMKWVTTSYYTDGLAASADEMNVNGQIEQYASSVWEPMYSPNTYLFPS
                     ANENKKNGFQYFPNSKRKGSNLPILKNTPNANALVADLALKIFEKENLGQDTYPDMLL
                     LQFNVKTPYEKTFALKTAEKEDMYLRLDKDLQYLFQKIDSKIGLSNTLIFILGTQTSA
                     HTPLELGENKIPAGYFNGRRSMALLNTYLMVLYGQERWVEGYYGKNIFLNRQKIEEKK
                     INLQEIQQVVADFMHEFEGIQSAYTSYQIVNLTDPSNREMSKLRNSFQKSSKGDVIIT
                     LLPGWLELDDNNNPIGESNAVISSTPVYFYGWNIEPKKIAAIHSIIDIAPTICKILKL
                     PFPNATTGKPLEEIINK"
     gene            11694..12869
                     /locus_tag="PLM04_06380"
     CDS             11694..12869
                     /locus_tag="PLM04_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HON02737.1"
                     /translation="MKKYDFDEIIDRTNTNAVKIEACRSMFGTSNVIPLWVADMDFRT
                     PDFILDSIRKRCEHPILGYSVPPKDYFPSIMNWLKENHGWEIKKEWLGFMPGVVPGLS
                     FAVQQFSAENDEIVIQPPVYFPFMHVVEKNNRKLIFNPLKEVDGKFQMNFDDLEKKIT
                     SKTKMLILCNPHNPGGRSWGKETLQQLAEICAKHHLIVVSDEIHADMTLPGNVHVPFA
                     SVSERAANISLTYMSPSKTFNIPALQTSYFIIPNEELRNGLKNFLDRSELHNGNIFAY
                     LSTIAAYTEGKWWKMQMLDYVQGNIDFVIQFLQNEIPQIKPMKPEASFLIWLNCSDLG
                     MNEEELFQFFVHKAGLGLNQGITFGPGGEQHLRLNVACPRSTLEKAMNQLKNAVRNL"
     gene            12880..13557
                     /locus_tag="PLM04_06385"
     CDS             12880..13557
                     /locus_tag="PLM04_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HON02738.1"
                     /translation="MWGTLVNALAVIVGGSAGLLLNKKMPQRIQTIYFQVIGLFTIGI
                     GITMVVGMKHILLIVMSLVIGVLIGEWMHLESRLEKWSESLKKRFQIGSEQFSEGFVT
                     AFLLFCIGSMTILGSIQEGLTGSPDLLFTKSLMDGFSAIILASAFGVGVIFSSIPLLI
                     FQGGLTLLAMAASSFFTPDLINGISNIGGILLIGLGINILEIKKLAITNMLPSLVVVI
                     LLIWGFA"
BASE COUNT         4386 a         2569 c         2552 g         4088 t
ORIGIN      
        1 aatacatgtg ggtttcatat aaaaagtttt accgatcaaa agcaatctaa atcaagaaaa
       61 agcggaaaaa acacgaaacg ctgaaaaaaa tgtaattttg caaaaaatat ttgataattt
      121 tgtccaatta atttttcaag ccatgttaca caaattttct gctgcaaata tgagactttt
      181 ttaaggaatt tccacttaaa aaccaaagaa aaatgatcac aaaaaaaaga attgcgaatt
      241 ttaaatggct atcgatgatt tcactgctga tgttcatcag cattcagcat tctgcgcaag
      301 cacaaacttc gagtattcca aacgaaattg tatcggcatt gagcaatggg gatgccaatc
      361 gtttgtcggc ttatctgaat gacaatgtcg aactctttat cgaaaacaaa aatgatattt
      421 acagccgcca acaagctgtt agcattctca atgatttttt tcatcgtttc aaagtttccg
      481 acttcaaagt catccatcag ggaaacaggg ataattcgag ttttattatc ggtattcttt
      541 ccacagctac cgaaaatttc agggtttata tactgacccg caaatcggat aacaaaacta
      601 ttattttaca actcagaata gaaacatcaa atgaatgaag attacaaaca actgattccc
      661 ttatggtttg ctgaagatat aggagacggt gatcacacta cgctggcatg tattcccgaa
      721 acggcgctgg gaaaatctca actgatcatc aaagaaaacg gtgtaattgc cggcgtggaa
      781 gtggcacgag aaatttttaa gactttcgat ccaacattga aaatgaatgt atttattact
      841 gatggaacag aagttaaaat cggcgatatt gctttcaatg tggaaggaaa aattcagtct
      901 ttgctgcaaa ccgaacgcct gatgctgaac gtcatgcagc gcatgagcgg cattgcaacg
      961 cgtaccagac agtatgtcaa agctttggaa ggaaccaaaa cccgtgttct cgatacccga
     1021 aaaacgactc ccggacttcg atttctcgaa aaagaagctg taagaattgg cggcggcgtc
     1081 aatcaccgtt ttggtttgta cgatatgatt ttgctgaaag acaatcacat agattttgcc
     1141 ggtggaatca aagaagccat tttacgtacc aaagaatatt tgaaaaacaa gggaaaaaat
     1201 ctaaaaattg aaattgaggt acgcaatttt gacgaaattg aacaggtgat ggcaattggc
     1261 ggcgtggacc gaatcatgct cgacaatttc aatattgaaa ataccaaaaa agctgtcgaa
     1321 ataatcggag gccgctatga aatcgaatct tccggcggca tcacttttga cacacttcgt
     1381 caatatgccg aatgtggcgt tgattttatt tcggtaggag cgcttactca ttccgtaaaa
     1441 agtctggata tgagtttcaa agcggatatt cccaatctta tcgaagggat tcataaaatt
     1501 ttttaatcaa ctcgaattac atttaatttt ttaaatcgat aaatggagat tattgaaacg
     1561 cctattcccg atttgttaat tattcggcca aaggtttttg aagatgcacg tggctatttt
     1621 tacgaaacgt acaatgaaaa aagttttatg gaagccggtc ttgatattca tttttgtcag
     1681 gacaatcaat ccaagtcggt ttatggggtt attcgcggat tacattatca attatcaccc
     1741 tgcagtcagt gcaagcttgt ttctgccatt cagggaacgg tttgggatgt ggcagtggat
     1801 ttgagagctt cttcccctac cttcggaaaa tggtttggca ttgaactttc tgctgaaaat
     1861 catttgcaat ttcttattcc caaaggtttt gctcacggtt tttccgtact aaccgaaaca
     1921 gctatttttg cctacaaatg cgatgcattt tacaaccctt cgctcgaaag aggaattctt
     1981 tacaacgatc cacagctggc tatagactgg aaaattcctg aagataaggc aattgtttcg
     2041 gctaaagatg ctaaacatcc gatttttagc aaagctgaaa agaattttta agttttgttt
     2101 ttaaataaat gtttttgaac aaataaataa atcatgcaaa ctattctcat caccggcagt
     2161 aagggacaat tgggcaacga acttcaaaaa gctgcaaaac gctttccaaa ctttcgctat
     2221 atttttaccg atgttgatga gctggatatt tgcaacaaat ctgcccttga taattttgta
     2281 aaggaaaacc ggattgaaat aattgttaat tgcgcagcct atacagccgt agataaggct
     2341 gaagaggagg ttgaaaaatg ttttgccatt aatgcacaag ccgtaaaaaa cattggagaa
     2401 attgctgaaa cttatcagat aaaagttgta catatttcta ccgattacgt ttttgacgga
     2461 aaatcttaca aaccttatac cgaagagatg gaggtttctc ccgatactgt ttacggaaaa
     2521 tcgaaactgc aaggcgaaaa atttttgcaa tcagtttgta atcaatatat tgttatcaga
     2581 acctcgtggc tctattcctc ctacggaaat aattttgtta aaaccatgct gcggctcgga
     2641 aaagaaagaa attccgtaaa agtgattttt gaccaaattg gaacgccaac ttatgcagcc
     2701 gacttggcag aaatcatttt gaaaattctt tcacaaaaca attttatttc aggcatttat
     2761 catttttcca atgaaggggt ttgttcgtgg tacgattttg ctactgccat tttcaaaatt
     2821 gcccatgtta attgtttggt tcatcccatc gaaacaaaag attatccggc cccagcgccg
     2881 cgtccctatt acagcgtgct gaataaatcc aaaattaaaa acacgtttgg actatcaatt
     2941 gcccattggg aggaaagttt ggaaaaatgt atcgaaatat taaaatctga gtattcatta
     3001 taatttttcc atcctcattt ttcgtacaac aaataagttt ttcatgcttt gcgcatgaat
     3061 tattttttgc cgaaaaaaac atggaaagta ttaaaacatc ggctgttttt tcatattttt
     3121 gcatatcaaa aaattaagaa caacttttac tcaaacaagc aaaaaatata tttacgcatg
     3181 ggaagagcat ttgaatacag aaaagcaaca aaaatgaagc gttggggaaa tatggcccgc
     3241 gtatttacaa aattgggaaa acagattacc atagctgctc gtgaaggcgg ccccgatccg
     3301 gatatgaacc ctcgcttgcg cgtattgttt cagcaagcca aaaaggaaaa tatgcccaaa
     3361 gaaaatgttg aaagagctat taaaaaagct gtttctaaag aggaggccga caattataag
     3421 gaagttatat atgaaggtta tgccccacac ggaattgcgg taattataga aaccgccact
     3481 gacaatccca cacgaacagt tgcaagtatt cgcagctatt tcaatcgtta tggcggttca
     3541 ttggggactt cgggttcagt tcaatttttg ttcgatcaca aatgtgtatt tagaattgca
     3601 cccaagtcca atctttctat cgacgatctg gagttggaat tgatcgacta tggtgtggac
     3661 gaggttattc aggatgacga agaaaacaat attgtcctat atggagattt tcaacattat
     3721 tcttccattc aaaaatatct ggaagaaaac aattttgaga tattcagtgc cgaatttgaa
     3781 tggattccca aagaaacaaa ggaactgaat gatgaacaaa aagctcaggt actgaaactg
     3841 atagaaaaaa ttgaagacga cgaagatgtt caaaatgttt ttcacaacat ggcggaataa
     3901 ttaatttcaa attttacaat cgaatcagtt tgattttttc cttttaataa aaatacatat
     3961 ctattcgacc attaaaaatt caattaactc ttcaattaat acttaaaaaa tggatataag
     4021 tgttttgttc cctgcattgg catccttaac atggcaacat attttgatga ttctcattgg
     4081 actaactctc atatatttag ctatttttaa agaatatgaa cccactttac ttttacctat
     4141 tggttttgga accattctgg taaatattcc actgagttcg gctattaatc aaatagatgg
     4201 caaaactgga gaaattattg aaggtatttt gtctgttttt tatgaaggag gaattgctac
     4261 cgaaatgttt cctttgctga tttttattgc aattggggct atgattgatt ttacgccttt
     4321 gttcaacaat ccgcatttgt tgcttttcgg agcagcagca caatttggta tttttatgac
     4381 catgttcata gccaccttat tcggattcga cttgaaagaa gctgcttcca taggaattat
     4441 tggcgctgcc gacggaccaa cttccatttt tgtcgcttcg cgttttgcac ctcatctttt
     4501 gggccctatt tccgtcgcgg catactcgta tatggcgttg gttcccatta ttcagcctcc
     4561 ggttatcaaa ttgctgacgt caaaaaaaga aagactcatc aggatgcagt acagttccaa
     4621 aaaagtttcc cggaaggcat tgatcgcttt tcccattgtt gtaacaatca tttcaggctt
     4681 aattgcccct gcttctcttt cattgatagg atttttaatg tttggaaatc tgattcggga
     4741 atgtggcgta ctcaacaaat tgtcgcaatc ggctcagaat gaactggcaa gtttggtaac
     4801 catattattg ggtatcacca tagccagtac catgacggct gaacgtttcg taacatttgg
     4861 aaccctcacc atcattgctt tgggattgct ggcttttgtt ttcgatactt ttggtggagt
     4921 aatattcgcc aaacttttaa acctgatttt gccggctaag aagaaaatga atccaatgat
     4981 tggggcttgt ggaatttctg ctttcccaat gtctgcaaga gtaattcata caatgggttt
     5041 gaaggaagat ccacacaatt tcctgctgat gcctgctgtc agtgcaaatg ttggcggtca
     5101 aattgggtcg gtagtagcag gtggacttat acttactttg gtaaatttat tcgcttaaaa
     5161 tattgaacct atgatcaata atgttgaagc tgctctcgaa ataggagcaa tcggtattac
     5221 aggcatattc atttttatgc ttatatttta tgccgctatc aaacttattt tgaaactttt
     5281 ccctgaaaag gaaaacaaag aagaatagga aggagttaaa tcgatttttg attttttcga
     5341 caaccagata tttagtaaaa acgcattttt aagagttatt tttaactgct taaaatgcgt
     5401 ttttttcaat ggaataaatt agtaaaggac taatattgtt ctttttcgtt gggaaaatcg
     5461 gaagatttta catccgcaat gtaatgttga atagcctgat tcatttgttc tcttaaattg
     5521 gcatatcttc tcaagaaacg tggagaaaag ccctgcgtaa gtcccagcat atcgtgaatg
     5581 accaacactt gcccatcaac tccacctccg gcgcctattc caataaccgg aatagagagt
     5641 tcggaagcaa tttgagttcc caactttgcg ggaatttttt ccaacaccaa agcaaaacaa
     5701 ccggcatcct ccagcaaatg cgcatcccgt ataagttttt cggcttcttc ttcttcctgt
     5761 gccctaacat tgtatgaacc atatttgttg atggactgtg gcattaatcc caaatgcccc
     5821 ataatgggaa ttccggcacc aataattctt tttaccgatt caatgatttc ttcgccacct
     5881 tccatcttta ttccgtcagc atgagtttct ttcataatac gaatggctga agcaattgct
     5941 tcgtgagaat ttccctgata actgccaaaa ggcatatcta cgacggcaag agctctttta
     6001 attccgcgta tggccgattt tgccagaaat atcatctgat cgagcgttat gggcaaagtt
     6061 gtggtatttc cgcaaataac atttgaagcc gagtctccca ctaaaattac atctattcct
     6121 gcttcatcca caatattggc cattgtgtaa tcgtaagctg taagcatact gattttttca
     6181 ccccgctgtt tcatttcgaa taaccgacgc gtggttactt ttctgttgtc ttcattgtgt
     6241 actgacatga taatttgatt tgaatttgct gcaaagttac atcaaaattt ggttttctgt
     6301 ctttttattt tattgctgaa aggaaaagaa accgatgata aaaatttcaa cgaaaaacaa
     6361 ttttttttac ctttgcattc aaacaatttt agtgaaacga tatgttctgt gattttgagt
     6421 tttgttttag ctttgaagtg tttcaaaaaa aattactgga taaatgagta aaattaccat
     6481 tcacagttac caagaactgg aagaatatgt tgggaaagaa ttgggggtat cggattattt
     6541 aaccatcaca caggaacaaa tcaataaatt tgccgatgcc acactggatt atcaatggat
     6601 tcatgtgaat gccgaaaaag caaaacagga aagtccttat aaaaacacta ttgcgcatgg
     6661 atatttgaat ctttccatat taccttatct ttggggtcag attatggaaa tccgaaattc
     6721 taaaatgacg ctcaattacg gaattgagaa tgctcgtttt caacaggcag tggtagtaaa
     6781 cgatcaggtg agagccaaag caaaattaaa atcgctggtc aatttgaaag gaatagccaa
     6841 agcggaaatt tacactgttc ttgaaattaa aaacagcaat aaaccggctt tggaaggaac
     6901 tttcattttt ttatatcatt ttgaataact catatatcag agaaacgcaa gaattaaaat
     6961 ttgtgccgat acaactctta gaaacatggt taaaggatac accgtagaat aagccacagc
     7021 ggggatatcc gaaccggcaa ttgaattgga ataggcaagt gctggtggat cggtcgtact
     7081 acctgcaaga agtcctatca aagtaaaata atttatcttt aagaatttcc ggccaattat
     7141 gcccacaata agcagtggaa gcaaagctat ggctgctgca catcccatcc aaagcaaacc
     7201 attggcagaa ataaccgttt cgacaaattt ttctcctgct cctataccca cactggcaag
     7261 aaacaaagaa attcctattt cacgcaacat ataattggca cttgtagtgg tgtaggttat
     7321 taatttcaac ttgtacccaa aacgtcctaa caaaatagcc acaatcaaag ggccacctgc
     7381 caatccgagt ttaaccgaaa ccggtatgcc tggaacaaaa aatggaatac tccccactaa
     7441 aacacctaat aaaattccga caaaaatagt tatgataggt ggttcgttca atttttgcaa
     7501 ggtattgccc aataattttt ccactttttc aatggcgtcc aattctccaa ctaccattac
     7561 tctgtctcct acctgtaata ccaaatcggg tgttcccaac aaatcaacac ctgaacgatt
     7621 tacacgggta atatttacac cgtaaattgt acgtaatttc agcgagccga tagtcttccc
     7681 gttgattttt ccgtgcgtaa tcacaattct tctggaaacc agtttttctt cggatatttt
     7741 ccaatccatt tccacttttt ctccaatata ggatgttatg cgttcaatat ctcgcgaaga
     7801 agcaactatc agcaaaatat cgtctttgta taacatggaa tcgccttgag gaataaaaaa
     7861 ttctccattc cgcattaaac gcgaaacaat aaatggcctt ccaacagccc gtttcacatc
     7921 cgtaactgta tgattaaaaa ccttttcatt ttttacctgt acagaaattt tttcgacctg
     7981 ttcttcgtcc acttcactgg ctttttttat ttgttcggtt tccttgtgaa aatccacttt
     8041 aaaaattatt cgcagtaata ccattgaaaa aataattccc aatactccta tcgggtaagc
     8101 taccgcataa cccaaagcaa ttggcggaat ttctgcaccc ggtgaagatc caaaaacatg
     8161 ccgtaatgct tcctgagcgg ctcccaagcc cggagtattg gtaaccgaac cggaaagtat
     8221 gccgaccagc atgggcatgg acactttacc atgaagaaga taatataggg ctatcactat
     8281 tcctacaccc agcagcacaa tcatggtcga caataaattg agcagcatcc cacctttttt
     8341 gaaagaagaa aaaaaagatg gtcctacttg aaggccaatg gaataaacaa acaaaatcaa
     8401 accgaaattt ttaataaact ccaaaacttc cggattcacg cttaatccca aatgacctac
     8461 aaataaacct acaaaaagga caaaagtaac tcccaacgaa atcccaaaaa ttttaatttt
     8521 tcccaaaagg atacccaaag aaatgacaaa agcaaacaac aaaatggtat gtgcaatacc
     8581 atcattcaac aaaagcgaaa ggaaccactc cataaaatta ttaaaaaatt gattttcaaa
     8641 caaataaatt aaaattgtcc ttattgaaaa atgaaagcaa atttaaacaa ttttcagcat
     8701 tggataaaat atatattcaa ttgcttctaa atttgatcga acacttcaat atacttaaaa
     8761 tgaaacagat aaacaaataa ttgtagcatt cgttttttat tcagacaaaa aaaattactt
     8821 ttgcattaaa aatagtggta catttcagaa ataaggtaca ttacaagcat atcaataaaa
     8881 taaacatgga agaaaatact ggtaaaaccg taaaaaaaag agtgcttttt atcgatcggg
     8941 acggtaccat tatagttgaa ccttctgaaa cttttcaagt tgacagcttg gaacagctcg
     9001 aatttttgcc cggcgttatc cgcaatttat attttattcg tcagagaacc gattttgagt
     9061 gggcaatggt gtccaatcag gatggtttgg ggacttcggc ttatccggaa gaaaatttcg
     9121 aaaaagttca atccaaaatg ttgaaaattc tcgaaaacga aggaataatt ttcgacaaaa
     9181 tttttattga caaatctctg cctgaagaga aattgccaac gcgtaaaccc ggaactgcca
     9241 tgttgaagga atatttttcg gcaaattatg acttggaaaa ttcgtatgtg gttggcgaca
     9301 gaataaccga tgtggaattg gcaaaaaatc tcggctgcaa agcaattctt ctcaatgaag
     9361 atcctgaaat tctgcaggaa tcgaagttga aaaaagtctg cgtgttgcaa acaacctctt
     9421 gggacgaaat agctgcattt atctttgcag gggaaagaac cgccaccata caacgaacta
     9481 cgaaagaaac cgatattttt attcacctga atttggatgg ctcaggaaaa ggaaatattt
     9541 ctaccggttt gcgttttttc gaccacatgc tcgaacaaat tgcccgtcat tcgggttgcg
     9601 acttgaccat tcaagtgaaa ggagatttgg aagttgacga acatcatacc atcgaagata
     9661 ccgctattgc tttgggagaa gcttttgcca aagcattggg agacaagcgc ggaatagaac
     9721 gctatggatt ctgcttgcca atggatgata gtctttgttt ggttgccatc gattttggag
     9781 gacgagcatg gctgactttc gatgccattt ttaaaaggga atatatcggc gattttccta
     9841 ccgaaatgat ttttcacttt ttcaagtcgc tgagcgatgc ggcaaaaatg aatctttacg
     9901 taaaagcact tggcgaaaat gaacatcaca aaatagaagg aattttcaaa gcattggcca
     9961 aagctattaa aatggctgta aaacgagata tttaccaatt tgaacttcct acaaccaaag
    10021 gaattatcta atttttaact tatcgaccta tcattattga tcatggattt taaaagaaat
    10081 tctgcaaaat ttttcttttt gattttttta cttatcgttc aatttccact ccacgcacaa
    10141 atttttcctg accagccgcg tttggtaatt ggtattgtgg tggacggatt acaggaaaaa
    10201 cacatcgatt tgctttggca acgttttgat gcaaacggtt ttaaaaaaat cattgaaaaa
    10261 ggtactgcct ttgaaaatat ggaatataac attgtgtcgg ccggaaatgc ttcggatatt
    10321 gcaaccctga tgaccggcac tacaccttat tatcacggag ttacaggaaa taatttctat
    10381 tccagaaaac agggaaatgt tcaatctgtg attttcgacg aaaacgaacc gggcatagga
    10441 acaaaacaaa ccgtttctgc ccatcaactt ttggccagta ccattacgga tgaattgatg
    10501 ctgacctatc cgaaacaatc aaaaacttat gctatcgcca tcaatgctga agatgccatt
    10561 atgttgggtg gacatacagc caacagcgct atgtggctcg acgacatcaa catgaaatgg
    10621 gtaaccactt cgtattatac cgatggtttg gctgccagcg ccgatgaaat gaatgtgaac
    10681 ggacaaatcg aacaatatgc ttcgtccgtt tgggaaccga tgtactcgcc caatacttat
    10741 ttgtttcctt cagcgaacga aaacaaaaaa aacggatttc agtattttcc caacagtaaa
    10801 agaaaaggat ccaacctgcc cattttaaaa aatacgccca atgccaatgc tttggttgcc
    10861 gatttggctt taaaaatttt tgaaaaggaa aatctgggac aggatactta tcccgatatg
    10921 ctgctcttgc aatttaacgt aaaaactccc tatgaaaaaa catttgcgct aaaaactgcc
    10981 gaaaaggaag atatgtattt gcgcttggac aaagatttac aatatttatt tcaaaaaatc
    11041 gacagcaaaa taggcttgtc caacacgtta attttcattc tgggtaccca aacctctgct
    11101 catacacctt tagaattggg cgaaaataaa attcctgcag gatattttaa cggacgtcga
    11161 tcgatggctt tgctcaacac ttatttaatg gtgctttacg gacaggaacg ttgggtggaa
    11221 ggttattacg gaaaaaacat ttttttgaat cgacagaaaa tcgaagaaaa aaaaatcaat
    11281 ttgcaggaaa ttcagcaggt agttgccgat ttcatgcacg agtttgaagg aatccagtcg
    11341 gcttacactt cttatcagat tgttaatctt actgatccat ccaacaggga aatgtcgaaa
    11401 ctgcgcaatt catttcaaaa aagcagcaag ggagatgtga tcattacttt gcttcccggc
    11461 tggctcgagc tcgacgacaa caacaatccc ataggtgagt cgaatgcggt tatttcttca
    11521 acaccagttt atttctacgg atggaacatc gaaccaaaaa aaattgcagc aatccattcg
    11581 attattgaca tagctcctac catttgcaaa attttgaaac ttccttttcc caacgcaact
    11641 accggaaaac ctttggaaga aattatcaac aagtaaaaat tttttagcag ataatgaaaa
    11701 aatacgattt cgacgaaatt atcgacagaa cgaacaccaa tgccgttaaa atagaagctt
    11761 gtcggtcaat gtttggaacg tcgaacgtta ttcctttgtg ggtagccgat atggattttc
    11821 gtactcccga tttcattttg gatagtatcc gtaaacgctg tgaacatccc attttgggat
    11881 attctgttcc tccaaaagat tattttccat ccattatgaa ttggctgaaa gaaaatcatg
    11941 gatgggaaat taaaaaagaa tggctgggat tcatgccggg tgtggtaccc ggactttctt
    12001 ttgccgttca gcaattttct gccgaaaatg atgaaattgt tattcagcca ccggtatatt
    12061 ttccttttat gcatgtggta gaaaaaaaca acagaaaatt gatattcaat ccgttaaaag
    12121 aggttgacgg aaaatttcag atgaattttg acgatttgga gaaaaaaatt acttcaaaaa
    12181 caaaaatgtt gattttgtgt aatcctcaca atcccggagg aagaagttgg gggaaagaaa
    12241 ccttgcaaca attggctgaa atttgtgcca aacatcatct gatagttgtg tcggatgaaa
    12301 ttcatgccga tatgacgttg cccggaaatg tgcatgtgcc ctttgcttcg gtatcggaac
    12361 gagcagcaaa tatcagtttg acatacatgt cgccaagcaa aacattcaat atcccagcgc
    12421 tgcaaacttc ctatttcatt attcccaatg aggagcttcg aaatggactg aaaaattttc
    12481 tcgatcgttc ggaactccac aacggaaata tttttgctta cctctctacc attgccgcct
    12541 acacggaagg aaaatggtgg aaaatgcaaa tgcttgatta tgttcaggga aatatcgatt
    12601 ttgtcattca atttttgcag aatgaaattc cacaaatcaa acccatgaaa cctgaagctt
    12661 cctttttaat ttggctcaac tgctcagatt tgggtatgaa tgaagaagaa ttattccaat
    12721 tttttgtaca caaagccgga ttgggtttga atcagggcat aactttcgga cccggaggcg
    12781 aacagcattt acggctcaac gtggcttgcc ctcgaagtac cttagaaaaa gcaatgaatc
    12841 aattgaaaaa cgcagttaga aatctataaa tcaatatcta tgtggggaac tctggtaaat
    12901 gcactggctg tgattgtggg tggaagtgcc ggtttgttgc taaataaaaa aatgcctcaa
    12961 cgtatccaaa ccatttattt tcaagtaatt ggacttttta ccataggaat cggtattaca
    13021 atggtagtcg gtatgaaaca tattttgctg attgttatga gtttggtgat tggtgttttg
    13081 attggggaat ggatgcattt ggaaagtaga ctggaaaaat ggagtgaatc actgaaaaag
    13141 cgttttcaaa ttggcagtga gcaattttcg gaagggtttg ttacggcatt tttattgttt
    13201 tgcattggat ccatgactat tttgggatct attcaggaag gactgaccgg ttcgccagat
    13261 ttgctgttca ccaaatcgct aatggatgga ttttctgcca tcattctggc ttcggctttt
    13321 ggtgtcggag taattttttc ttccattccg ctccttattt ttcaaggtgg acttacactg
    13381 ctggctatgg ctgcatcttc tttttttact cccgatttga tcaacggaat ttccaacatt
    13441 ggcggaattt tactgattgg tttaggaatc aatatactcg aaattaaaaa acttgccatc
    13501 accaacatgc tcccttcact cgttgtagtt atccttttaa tatggggttt tgcataaaat
    13561 tcgttgtaaa ttaaaaattt cgctcctgaa tttca
//
LOCUS       DAOTAX010000053        13234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3940_length_13234_cov_28.986190, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000053 DAOTAX010000000
VERSION     DAOTAX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_13234_cov_28.986190"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLM04_06390"
     CDS             <1..222
                     /locus_tag="PLM04_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02739.1"
                     /translation="YEKELREKEVEGIINLKDHQIQTLQSKIKEMEQQLREASSKVDV
                     SEKTVKDIALKAIESAGKTQFVEREKPRE"
     gene            396..1613
                     /locus_tag="PLM04_06395"
     CDS             396..1613
                     /locus_tag="PLM04_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02740.1"
                     /translation="MNTELENNFFKSTSSSDSSEAVVNVNAHLHTPYSFSAFRDIDDA
                     LDRAASENVKVVGINDFYSTAGYDEWDEGCSRRRLYPLFGIEFIALDEVDQKAGLRVN
                     DPANPGRTYISGKGLRQPFFLAEPFASQLQTVREASNRQVEAMCFKLNEVLEQKKIGF
                     LIDFDQIAFELTKGLIRERHLAKALRLKVYEYCQNDASKIQSVLETIFDGKPLKSSVN
                     DWAGVENEIRSNLLKAGGSAFVPEESTAFLPVKSVCNIILAGGGIPTYPFLADDAKGN
                     YTDFENDLERVSRQLTERGFHSVEFISTRNDLHLLEKYAEYLYDQGFVVTFGSEHNTP
                     AMEPILLSARNGIPLSEKLKKINYEGACVIAAHQHLVKQGLRGFVDENGFADRSKRSE
                     FVNLGKELIDRVL"
     gene            1816..3777
                     /locus_tag="PLM04_06400"
     CDS             1816..3777
                     /locus_tag="PLM04_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HON02741.1"
                     /translation="MKEIEQLIEISQFYGRDSRYVIAGGGNTSYKNADRIWVKASGAS
                     LATITEDGFALLDRTKLRPMSEKKYSDDVALREEEVKNDLAAATLTRDKRPSVETSMH
                     NIIEYAFVVHLHPTLVNALMCSQQAESMLKKLFGEKALFVEYTDPGYVLFKKVEEKIK
                     AFRAAYDEEPHVIWLQNHGIFVAADTIEEIKKIYSDIFSTLEKSLQKTLPSNETLASH
                     DAEKVIPAVRMMLSTNGLKTLKVRNNPIIAFFSENVENRKLIAKPFTPDEIVYCKSNY
                     LFFDGNSEETLLREAETQIPVFIEKQGYHPHVLLMKGIGLVAAGLNAKQCETILDVFE
                     DAMKVAYLAQSFGGEHPMTQNQIDFIDHWEVENYRRSVSTAKSNGRTENKTIVITGAA
                     QGFGEGIARCLVKEGANIVVADLNEETGRKTAGELNMLCGGNRVVFVKTDVTNIASLE
                     SLMYDAVCNFGAIDCYISNAGVLRAGGLDEMTPETFDFVTRINYYGYFYGVKVVSRTL
                     KLQTRFAPKNYYADIIQINSKSGLKGSKANFAYAGGKFGGIGLTQSFALELAPFRIKV
                     NAVCPGNFYEGPLWSDPDNGLFVQYLKAGKVPGAKTIEDVKTYYLAQVPMKKGCTPED
                     VTKGVLYLMEQCGETGQALPITGGQVMLA"
     gene            4172..5449
                     /locus_tag="PLM04_06405"
     CDS             4172..5449
                     /locus_tag="PLM04_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HON02742.1"
                     /translation="MKTKAVRLYGKKDLRLEEFDLPKIKDDEILAKVVSDSLCMSSYK
                     AAIQGADHKRVPDNVAENPVIIGHEFAGELIEIGSKWADQFKVGDKFSIQPALNYEGG
                     SVGILSAPGYSYPFIGGDATYVIIPNEVMEKNCLLPYHGEGFYPASLAEPLSCVIGAM
                     HANYHLKPGSYEHKMGIVDGGKMAILAGVGPMGLAAINFVLHRKDRRPSLLVVTDIDQ
                     ARLDRAASLYTVEFAASHGIDLRYINTSVMQNPIEELKTLTNGNGYNDVFVFAPVKSV
                     VELADAILGFDGCLNFFAGPSDPKFSAMLNFYNVHYAYTHVAGTSGGNNEDMVEALDI
                     MNKGLDPAGLVTHIGGLNAVSEATLHLPEIPGGKKLIYTHITMPLTSISDFAELGKTS
                     EFFRELSSICARHKGLWNVEAEAFLLANANKSN"
     gene            5472..6770
                     /locus_tag="PLM04_06410"
     CDS             5472..6770
                     /locus_tag="PLM04_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HON02743.1"
                     /translation="MKQLDVRLMHPVEQINVIIKRIYKSGMTTTSGGNISIRDQNGDI
                     WITPSGVDKGSLTFNDIMCVKKDGTVIGPHKPSSEFPFHKAIYETRPELTAIIHAHPP
                     ALVAFSIAHKVPDTKIVPQAHNICGEIGFAPYGTPGSDDLGKKIANEFRNTQYKAVIM
                     ENHGVVLGGMDLMDAYQRFETLEFCCRTIVNASRLGEVNYLSDEQVARYVNHLPSNIP
                     YFMNVDYPSDERALRSEMVKIIRRACDQGLMISTYGTVSVRWRDDDFLITPKNVARWD
                     IVPEDIVQIKKGMAEVGKTPSRSVALHQRIYQLNPQINSIICTQPVNLMAHAITGTKF
                     DVRTIPESWIFLQDVPSIPFGLIYNDIDSVAEMFNHNKVILVENDSVFVTGDKLLNTF
                     DYLEVAEFSANSLVMASAIGHLCPISDQEIDDLRVAFNVQ"
     gene            6802..7536
                     /locus_tag="PLM04_06415"
     CDS             6802..7536
                     /locus_tag="PLM04_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HON02744.1"
                     /translation="MQVVFSPYKIPEFNLAAEEFLFSEKTDEYLLLYVNKPCVILGSN
                     QSVTNEVNEDFCHLNGISIFRRLSGGGAVYHDEGNLNFCFIFNKKKGYSPLSSDFLQP
                     VISVLNSMEIPVELGKRKDLWLPDGHKVSGTASHVSKCRELHHGTLLYDVDMEKLQKS
                     LIPTSATNKKAIRSVPSPVENIRSFLERSTVRTQDVNDFFITFTKLMLAYFQIRELSL
                     LLNEDIKKIYRLSISKYGRKEWNYRI"
     gene            7933..9960
                     /locus_tag="PLM04_06420"
     CDS             7933..9960
                     /locus_tag="PLM04_06420"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HON02745.1"
                     /translation="MTYESEKITRKKRIDQQLKASGWLIIPYSEGMDLTILTNHAIEE
                     LQTNSGPADYALVVNGKLLGVVEAKKLEVGAANVLEQAKRYSKGANKTVGEWNEYRVP
                     FLYSSNGELIYYLDARDSKNLSRQIYSFHTPEAMETLFNSNKETALQWLKEKPINTPG
                     LRPYQKEAIQAFENNLEERKRLMLLAMATGTGKTFTTVNLVYRMLASGYAKRILFLVD
                     RKSLAAQAVTAFASFDTPRNIKFKDEYELYSQRFKKEDFEGEAYDPTVLPNSYLTNPD
                     GTKTFLYICTIQRMAINLYGKAGAFGENEENEDDAEKLSIPSHAFDLIIADECHRGYT
                     SKETNVWRNVLNHFDAVKVGLTATPASHTVAYFNKPIFNYSLQQAIEEGYLVDYDAVA
                     INSEVLMNGAFLKEGEQVGMIDTETGREQIDQLEDEREFTSTEIEEKITAPDTNQKIV
                     EELKKYTDEWEKRTGRFPKTLIFASNDIPMISHADRLVSICKSVFNRGDDFVAKITGS
                     PTVDRPLKKIKEFRNRPNPKIVVTVDMLSTGVDIPSIEFIVFLRPVKSRILWEQMLGR
                     GTRKCDEIGKTHFVIFDCFNGTLIKYFKDASYNFKIDPPGTETITIKELIEKIYNNED
                     RKANAKRLAKRLHRIEKDMSGNARDKFATYIPNGDMGKFAELLAKFFEKYK"
     gene            9960..10646
                     /locus_tag="PLM04_06425"
     CDS             9960..10646
                     /locus_tag="PLM04_06425"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HON02746.1"
                     /translation="MAKSFNEIFESFTSVMELLRNEQFQKLLVDYERAKKVFLVGYEV
                     KDDVSSMVLFEAEGRYGLKPTDYLLAFSEFVKRKENEIAAIGILLNKPNDWNTKALNE
                     LKQKLKENNYDEANLQKAHKFVYHKETVDIISMIKHAAKDTEPLLSPDERVNQAIQKV
                     TAGKNLNEEQQKWMEYIKEHLKQNLTLDEDDFKELPVFTDRGGLNKFKKVFADEYQKI
                     IKEINNAIAA"
     gene            10657..>11096
                     /locus_tag="PLM04_06430"
     CDS             10657..>11096
                     /locus_tag="PLM04_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HON02747.1"
                     /translation="MKKELILELFQKFENICYLYNDVECWSARELQALLGYSKWENFE
                     KVIVKAKESCSKAGENIEDHFPDVRKTIPMPKGAEKQIDDILLTRYACYLIAQNGDAR
                     KEEISFAQNYFAVQTRRAELVEKRLLEFERVKAREKLSQTEKQLS"
     assembly_gap    11097..11196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11197..11519
                     /locus_tag="PLM04_06435"
     CDS             <11197..11519
                     /locus_tag="PLM04_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HON02748.1"
                     /translation="MGTIQLKKKLGVPDSRPVADFLPTISIKAKDLAAEMTGLNVQNK
                     DLKGQSLIEKEHVDNNLAVREMLTKRGIFPENLPPAEDVKKLQRKLEGEEKKILTKKR
                     RNNE"
     gene            11512..12912
                     /locus_tag="PLM04_06440"
     CDS             11512..12912
                     /locus_tag="PLM04_06440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HON02749.1"
                     /translation="MSDVVNKLWGFCHTLRHEGIDYNDYIEQLSFLLFLKMADEKEVH
                     LNDKYNWKALKELSGGDLMDHYTDSLKELAKEPGLLGEIFTQAQNRFNNPVSLKKLLN
                     LIDEDEWTSMDIDVKGAAFEGLLEKAASEGKKGAGQYFTPRPLIQSMVRLMKPDPRGK
                     NTFTIYDPACGTGGFLVCAYEWLLQECKGAFPREDVKRIRENTYYGQELVARPRRLAL
                     MNMYLHGVTPKIKLGDTIYNNPSSDRFDVILANPPFGTKGANQIPERDDFTVETSNKQ
                     LNFVQHIMSTLKIGGRAAIVLPDNVLFEDKAGEVFEILMQECNLHTILRLPRGTFTPY
                     AQGVKANVIFFQKGLPTEKVWIYDNRSNIESITKKDRPLTAKHFEDFEKCYGSDPNGN
                     SPRTDQGETGRFRCFTIDEIKKRNYKLDITWLKDESIEDADSLPEPNDLATEAIAELE
                     AVVDDLKEIISLLEKE"
     gene            12924..>13234
                     /locus_tag="PLM04_06445"
     CDS             12924..>13234
                     /locus_tag="PLM04_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HON02750.1"
                     /translation="MYILECSNGMYYTGSTNNLELRLQQHQNGEGANFTKKHLPVKLV
                     YFEEFARVDEAFYREKQIQGWSRKKKEALINNQFEKLHDFAECKNETHFLNVPFDSAQ
                     GT"
BASE COUNT         4399 a         2260 c         2845 g         3630 t
ORIGIN      
        1 tacgaaaaag agcttcgcga aaaagaagtg gaaggaatta tcaatctgaa agatcatcag
       61 attcagacgc ttcaatccaa aataaaggaa atggaacagc aattgcgaga agcatcgtcc
      121 aaagtggatg tttcggaaaa aacggtgaaa gatatagctc tgaaggctat cgaaagtgcc
      181 ggcaaaacgc agtttgttga acgcgaaaag ccgagagagt aagagaacca agagtcaaga
      241 gtcaagagcc tggagtcaag aagtgaagaa ataagaagtg agaacgtgaa gagaaaggtc
      301 gcatcagcac attattcaca tcagcaaatc aacaaatcaa caaaaaagag aaggcctcaa
      361 aggtatatct cggtttatgc tcgacacatt aaaaaatgaa tacggaattg gaaaataatt
      421 tctttaaaag tacttcctca agcgatagta gtgaggccgt cgtcaatgtg aatgcccatc
      481 tgcatacgcc ctattctttc agcgcttttc gcgatatcga cgatgcgctc gacagagctg
      541 catccgaaaa tgtgaaagtt gtcggaatca acgattttta ctcgacagca ggatacgacg
      601 agtgggatga gggctgtagc agaagaaggt tatatccgct tttcggaatc gaatttatcg
      661 ctttggacga ggttgatcaa aaagccggtt tgcgggtgaa cgatcctgcc aatccgggtc
      721 gtacttacat aagtggtaag ggtttgcgac aacctttttt cctcgcagaa ccttttgctt
      781 cgcaattgca aaccgttcgt gaagcttcca acagacaagt ggaggcgatg tgtttcaagc
      841 tcaacgaagt gcttgaacag aaaaaaatcg gattcctaat cgatttcgat cagattgctt
      901 ttgagttgac aaaaggatta atccgcgaac gacatttggc aaaggctctc aggttgaagg
      961 tttatgaata ttgtcaaaac gatgcaagta agattcaatc ggttttggag acgatttttg
     1021 atgggaaacc gctgaaatct tcagtgaacg attgggccgg agtggaaaat gaaattcgtt
     1081 cgaatttgct caaggcgggt ggctcagcat ttgttcccga agaatcgacg gcgtttcttc
     1141 ccgttaaatc ggtttgtaat attattctgg caggaggagg aattcctact tatccgtttc
     1201 tggcagatga tgcaaaaggc aactataccg atttcgaaaa cgatttggag cgcgtgtctc
     1261 gtcaacttac cgaaagagga ttccattcgg tagagtttat ttcgactcga aacgatttgc
     1321 atttactcga aaagtatgcc gaatatcttt atgatcaagg ctttgtggtt actttcggaa
     1381 gtgaacacaa tacgccggca atggagccga ttttgctttc ggctcgaaat ggaattccgc
     1441 tttctgaaaa attgaaaaaa ataaattacg agggtgcatg cgtaattgcc gcccatcagc
     1501 atttggtaaa acaaggcttg cgaggttttg tggacgagaa cggttttgcc gatcgttcca
     1561 aacgtagcga atttgtgaat ttggggaagg aattaatcga ccgagttttg tgatttggat
     1621 tgaagaattt acgccacaag gacgcgaagg cgcaaagaaa caaaaagaat ttgaggaact
     1681 tagtgtctta gtgccttcgt ggcaattaaa aaatttattt gatcgctgat gagtttgttt
     1741 cccaaaaacg cgaagtcacg aaaaaaagac aaaagaattt agagaaaatt gacccatagg
     1801 gagcaaaaaa caaaaatgaa agagatagag caattgatag aaatatcgca attttatggc
     1861 cgagacagtc ggtatgtgat tgcgggagga ggaaatactt cgtataaaaa tgcggataga
     1921 atttgggtaa aggccagtgg agcatcgctt gcgaccatca ccgaagatgg atttgccctg
     1981 ctcgacagga caaaacttcg tccgatgtcc gaaaaaaaat acagcgacga tgtcgctttg
     2041 cgcgaagaag aggttaaaaa cgacctggca gccgccacgc ttaccaggga taagcgtccg
     2101 tcggtggaaa cttcgatgca caacatcatc gaatatgctt ttgtcgtgca tttgcatcct
     2161 acgctcgtga atgcactgat gtgttcgcaa caagccgaat ccatgctaaa aaaacttttc
     2221 ggtgaaaaag cactttttgt ggaatatacc gatccgggtt atgtgttatt caaaaaagta
     2281 gaagaaaaaa taaaagcgtt tcgcgctgct tacgacgaag agcctcatgt tatctggttg
     2341 caaaatcacg gaatatttgt agctgcagat accatagaag aaatcaagaa aatttattct
     2401 gatattttca gcacactcga aaaatcgtta caaaaaacgc ttccttcgaa cgaaaccctt
     2461 gcaagccacg atgcagaaaa ggtgattcct gcggtcagaa tgatgctttc aaccaatgga
     2521 ttgaaaacac taaaagttcg caataatccg ataatagcgt ttttttcgga gaatgtcgaa
     2581 aaccgaaaac tgattgcaaa acctttcact cccgacgaaa ttgtatattg caaatcgaat
     2641 tatctgtttt ttgacggcaa ttcggaagaa actttgcttc gcgaagccga aacgcaaatt
     2701 cctgttttta tcgaaaaaca gggatatcat cctcatgttt tgctgatgaa gggaatcgga
     2761 cttgttgctg ccggcctcaa tgccaaacag tgcgaaacca ttctcgacgt gtttgaagat
     2821 gccatgaaag tggcttatct ggcgcaatcg tttggtggag aacatccaat gacgcaaaat
     2881 caaatcgatt tcatcgatca ttgggaagtc gaaaattatc ggagaagtgt gtcaacggca
     2941 aaatcgaatg gtcgtaccga aaacaaaacc atcgtgatta caggagctgc acaaggtttt
     3001 ggcgaaggta tcgcgcgctg tctggtgaaa gaaggtgcca atattgtggt agccgatttg
     3061 aatgaagaga caggtcgaaa gacagccggc gaattaaata tgctttgcgg gggcaatcgg
     3121 gttgttttcg tgaagacgga tgtaacgaat attgcaagtc tcgaaagttt gatgtacgat
     3181 gctgtttgca atttcggtgc catcgactgt tacataagca atgctggcgt ccttagggcc
     3241 ggcggattgg acgaaatgac acccgaaacg ttcgattttg ttactcgaat caattattac
     3301 ggatattttt atggcgtaaa ggtcgtgagc cgaaccttaa aattgcaaac tcggttcgcc
     3361 ccaaaaaatt attatgctga cattatccag atcaattcca aatctggatt gaagggaagc
     3421 aaagccaatt ttgcttatgc cgggggcaaa tttggtggaa ttggtttgac tcagtcgttt
     3481 gcattagaat tagctccttt ccgcatcaaa gtgaacgctg tttgtcccgg taatttttat
     3541 gagggaccgc tttggagtga tcccgataac ggtttatttg ttcagtattt aaaggcagga
     3601 aaagttcccg gtgcgaaaac gatagaagat gtaaaaacat attatttggc tcaggtgccg
     3661 atgaagaaag gttgtacccc tgaagatgtt accaaagggg tcctttatct gatggaacaa
     3721 tgtggcgaaa ccggacaggc actcccaatt actggtgggc aggtgatgtt ggcatgatat
     3781 aaaaatcaag aatcaagagt caagagaaaa ttgaaatatt actttgcgca acttcgtgtt
     3841 tttgtgtctt ggtggcaaaa acagacacaa aggctccaag acacaaagaa aacacgaaac
     3901 taaaaactta agaatacgtt attacgtaat taatattgct cgagtgtctt ttcaatccta
     3961 tgtcaacctc gaaccttacg aacaattaca acaaaaactc tcagaattgc agcgaatgat
     4021 acatgacttc aaaaattctc ttatgtcttg attcttgact ctaaaaaaca aaaaaatatg
     4081 aaacccacat gtattacata cacaaacacg aatgaatata atattcagac atgtggattc
     4141 catcagacct ttaaaaataa attatattct gatgaaaaca aaagcagtta gattatacgg
     4201 taaaaaagat cttcgtctgg aagaatttga tttgcccaaa atcaaggatg acgaaatttt
     4261 agccaaagta gtttccgatt cgttgtgtat gtcatcctac aaagcggcca ttcagggagc
     4321 agatcacaaa cgggtgcccg ataacgtggc cgagaatccg gtgatcatcg gtcatgagtt
     4381 tgcaggtgaa ttgattgaaa ttggttcgaa gtgggcagat caatttaagg tcggcgataa
     4441 attttccatt cagccggcgt tgaattacga aggcggttcc gtaggaatat tgagtgcgcc
     4501 gggatacagc tatccgttta ttggtggtga tgctacgtat gtgatcattc ccaatgaagt
     4561 aatggagaaa aattgtttgt tgccttatca tggggaagga ttttatccgg catcgctggc
     4621 agagcctctt tcttgtgtga ttggcgccat gcatgccaat tatcatctga agcccggtag
     4681 ttatgaacac aaaatgggaa ttgtggatgg cggaaaaatg gctattctgg ctggtgtggg
     4741 accaatggga ttggctgcta tcaattttgt gctacacaga aaagatcgtc gcccttcgtt
     4801 gttggtagtt accgatattg atcaagcacg tcttgatagg gctgcttcgc tctataccgt
     4861 tgaatttgcg gcttcacatg gtatcgatct tcgttatatc aatacttcgg ttatgcaaaa
     4921 tccgatagaa gaattaaaaa ctttaaccaa cggtaatggc tacaatgatg tatttgtttt
     4981 tgctccagtg aaatcggttg ttgaattagc agatgccatt cttggattcg atggatgcct
     5041 caactttttt gcaggaccaa gcgatcccaa gttcagtgcc atgttgaatt tttacaacgt
     5101 tcactatgcc tatacgcatg ttgcgggtac aagtggggga aacaacgaag atatggtaga
     5161 agccttggat ataatgaata agggtcttga tcctgcaggt ttagtaacgc atattggagg
     5221 tttgaatgct gtatccgaag ctactcttca tttgcctgaa attccgggag ggaaaaaatt
     5281 gatttatact catattacca tgcctttgac gtcaatctca gattttgccg aattaggcaa
     5341 aaccagcgaa tttttcagag aattgtcttc catttgtgca agacataagg gattatggaa
     5401 tgtggaagct gaagcttttt tgttggcaaa tgcaaacaaa tcaaattaaa ttaaattata
     5461 aactatagaa tatgaaacaa ttagatgtta ggctgatgca tccggtggaa caaatcaatg
     5521 tgatcatcaa aagaatttac aagagtggta tgaccacaac ttcgggtgga aatatttcta
     5581 tcagggatca aaatggcgat atatggatta ctccatctgg agtagacaaa ggatcgttaa
     5641 cttttaacga tatcatgtgc gtgaaaaaag acggaacggt tatcggtccg cacaaacctt
     5701 cttctgagtt tccctttcat aaagccattt acgaaacgcg tccggagttg acagcaatta
     5761 ttcacgccca tcctcctgca cttgtagcct ttagtatagc acataaagtt ccagacacaa
     5821 aaatagttcc tcaagcccat aatatttgtg gagaaatagg ttttgctcct tatgggacac
     5881 cggggagtga tgatttggga aagaaaattg ctaacgaatt caggaatacg caatataaag
     5941 cggtaatcat ggagaatcat ggggttgttt tgggagggat ggacttgatg gatgcctatc
     6001 agcgttttga aacacttgaa ttttgttgcc gcacaattgt gaatgcaagt agattaggag
     6061 aagtaaatta tttatcggat gaacaggtcg caagatacgt taatcatctt ccttccaata
     6121 tcccatattt tatgaatgtg gattatcctt ccgacgagag agccttgcgt tcggaaatgg
     6181 taaaaattat tcggagagca tgtgatcagg ggcttatgat ttctacctac ggcacagtgt
     6241 cggtacgttg gcgagacgac gattttttga ttacgccgaa aaatgtggca cggtgggata
     6301 ttgttcccga agatattgtg cagattaaaa aaggaatggc tgaagtaggg aaaacgccta
     6361 gccgttcggt agccttacat caacgaatat atcaattgaa cccacagata aattccatta
     6421 tttgcacgca accggtaaat ttgatggcac atgccataac cggtacaaaa tttgacgtgc
     6481 gcacgattcc tgaaagttgg atttttcttc aggatgttcc gtccattcct ttcggtttga
     6541 tttataatga tattgacagc gttgctgaaa tgtttaacca caacaaagtc attttggtgg
     6601 agaacgacag tgtatttgtt accggagata aattgttaaa cactttcgat tatttggaag
     6661 tggccgagtt ttcagccaat tcacttgtaa tggcttcggc tattgggcat ctttgtccta
     6721 ttagcgacca ggaaatagat gatttgagag tggcatttaa cgtacaataa ggataggata
     6781 aaaaaagtct gaattttatc aatgcaggtt gttttttctc catataaaat accggaattt
     6841 aatttggctg ccgaagaatt tcttttttcg gaaaaaacag atgaatatct tttgctttat
     6901 gtaaacaaac catgtgtaat tttaggctct aatcagtccg taaccaatga agtgaatgaa
     6961 gatttttgcc acttaaacgg tatctccatt tttcgacgat tgtcgggagg aggtgccgtg
     7021 tatcatgatg aaggaaattt gaatttttgt tttattttca ataagaaaaa aggatattct
     7081 ccgttaagtt cagatttttt gcagcctgtg atttctgttc taaattcaat ggaaattcct
     7141 gttgagctgg gtaagagaaa agacctttgg cttccggatg gacataaggt ttcaggaacc
     7201 gcctcacatg tgtcaaagtg tagggaattg catcatggta ccttgcttta cgatgttgat
     7261 atggagaagc tacaaaaatc actgattcca acctcagcaa ccaataaaaa agcaattcgt
     7321 tctgttcctt caccagtaga aaatattcgc agttttttag agagatcgac cgtaaggact
     7381 caagatgtca acgatttttt catcactttt acaaaattaa tgctcgctta ttttcagatt
     7441 cgagaactct ctcttttatt aaatgaagat ataaagaaaa tctatagact tagtatttca
     7501 aaatacggcc ggaaagaatg gaattatagg atatgaaact tttccaaacc aaaaaattag
     7561 attgtattgt tcattaattt catcagaata taatttattt tttaaatgtc tgatggaacc
     7621 cacatgtctg aatattgtat tcattgtggt tgcgtatgca caagcggtga aaagacaacg
     7681 acacagcaaa ttgaaaatgg aatattaaac ttgaaatgaa aaaagaaact gacagacata
     7741 taacccgaca ctcattgctt aaccaaatat gttttaattt ttttacccac cacacaaaaa
     7801 ttttgaaatg cgaagcattc atgaataaca ttgaaaataa aaatagcgtt agtaaattat
     7861 attgccagcc cacaaatggc acattttttt gccattgcac cgagaattat ttgtaccttg
     7921 cgatttagaa ttatgacata cgaatcagag aaaataacaa gaaaaaaaag aatagaccag
     7981 cagctaaaag cttctgggtg gcttattatt ccttactctg aaggaatgga cttgactatt
     8041 ttgacaaatc acgctattga agaattacag actaatagcg gacctgctga ctatgcttta
     8101 gtagttaatg gaaaacttct tggagttgtt gaagcaaaga aacttgaagt tggtgcagcg
     8161 aatgttttag agcaagcaaa acgatattct aaaggagcaa acaagacagt tggtgaatgg
     8221 aatgaatacc gagtcccgtt tctatattct tctaatggag aattgattta ttatttggat
     8281 gccagagata gtaaaaatct tagcagacag atttatagtt ttcatactcc cgaagcaatg
     8341 gaaactttgt ttaattcgaa caaagaaaca gctttacagt ggctaaaaga aaaaccgata
     8401 aatactcctg gactaagacc ttatcaaaag gaagcaatac aagcatttga aaataattta
     8461 gaagaaagaa aacgcttaat gttattagca atggcaactg gtacaggaaa aacttttacc
     8521 acagttaacc ttgtttatcg gatgttggct tcgggttatg ctaaacgaat tttatttttg
     8581 gttgaccgaa aatcattggc tgctcaagcc gttactgctt ttgcttcgtt tgatacacca
     8641 agaaatatta aattcaaaga cgaatacgaa ttgtattcac agcgatttaa aaaagaagac
     8701 tttgaaggcg aagcatacga cccgactgtt ttaccaaatt cttatttaac taatcctgat
     8761 ggaacaaaaa catttcttta tatctgcacc attcagagaa tggcaataaa tctttacgga
     8821 aaagctggtg cttttggtga aaatgaagaa aacgaagatg atgctgaaaa actttctatt
     8881 ccatcacatg cttttgattt aattattgct gacgagtgcc atcgtggtta cacatcaaaa
     8941 gaaaccaatg tttggagaaa tgttttaaat cattttgatg ctgttaaggt tggtttgact
     9001 gctacgcctg catcacatac tgttgcttat ttcaataaac cgattttcaa ttattcgtta
     9061 caacaagcaa ttgaagaagg ttatttggtg gactatgatg ctgttgccat caattcggaa
     9121 gtgcttatga acggagcatt tttgaaagaa ggcgaacaag tgggaatgat tgatactgaa
     9181 acgggcaggg agcaaattga ccaattggaa gatgaaaggg aatttacttc aaccgaaata
     9241 gaagaaaaaa tcacggctcc cgacaccaat cagaaaatcg ttgaagaact taaaaaatat
     9301 actgacgaat gggaaaaaag aacaggtcgt ttccctaaaa cattgatttt tgcttcaaac
     9361 gatattccaa tgatttcaca tgccgaccgt ttagttagta tttgcaaatc agttttcaat
     9421 cgaggtgacg attttgttgc aaaaattaca ggaagcccaa cggttgacag acctttgaaa
     9481 aaaatcaaag agtttcgtaa ccgacccaat ccgaaaattg ttgtaacagt tgacatgctt
     9541 agtacaggcg tagacattcc ttcaatagaa tttattgttt ttcttcgtcc tgtaaaatct
     9601 cgcattcttt gggaacagat gcttggcagg ggaaccagaa aatgtgatga aattgggaaa
     9661 acacattttg taatatttga ttgttttaat ggtactttga taaaatattt taaagatgcc
     9721 agttataact ttaaaattga cccgcccgga acggaaacta ttacaattaa agaactcatt
     9781 gaaaaaatat acaacaatga agacagaaag gctaacgcta agcgtttagc aaagcgactt
     9841 cataggattg aaaaagatat gagcgggaat gcccgtgata aatttgcaac ctatattccc
     9901 aatggcgaca tggggaagtt tgccgaacta ttagccaaat tttttgaaaa gtataaataa
     9961 tggcaaagtc gtttaatgaa atatttgaaa gcttcacatc agtgatggaa ctactcagaa
    10021 acgagcagtt tcaaaaacta ttggtggact acgaaagagc taaaaaggtt tttcttgttg
    10081 gatacgaagt aaaagacgat gttagttcaa tggtcttgtt tgaggcagaa ggcagatatg
    10141 gtttaaaacc aacagattat ttacttgctt tttcagaatt tgtaaaacgt aaagaaaatg
    10201 aaattgcagc aatcggtatt ttattgaata agccgaatga ttggaatact aaagcattga
    10261 acgagctgaa acaaaaactg aaagaaaaca attatgatga agccaatttg caaaaggcgc
    10321 ataaatttgt ttaccacaaa gaaactgttg atattattag catgattaaa catgcagcaa
    10381 aagatactga acctttgtta agtcctgatg aaagggtgaa tcaagcaata cagaaagtta
    10441 cagcaggtaa aaatttaaat gaagaacaac aaaaatggat ggaatacatc aaagaacatt
    10501 tgaaacaaaa cctgacttta gatgaagatg attttaagga attgccagtg ttcactgaca
    10561 gaggcggttt aaacaaattt aaaaaggttt ttgcagatga atatcaaaaa attattaaag
    10621 aaataaacaa tgcaattgca gcataatgtg taagttatga agaaggaact cattttagaa
    10681 ttgtttcaga agtttgaaaa catttgttat ttgtacaatg acgtggaatg ttggagcgca
    10741 agagaactac aagctctgct gggttatagc aaatgggaga attttgagaa agtaattgtt
    10801 aaagcaaaag aatcctgttc taaggcgggt gaaaatatcg aggatcattt tcctgacgtc
    10861 aggaaaacga tacctatgcc taaaggagct gaaaaacaga tagatgatat tctgcttacc
    10921 cgatatgcct gttatttaat tgcacaaaat ggcgatgcga gaaaggaaga aatttcattt
    10981 gcacagaact attttgctgt tcaaacccgc agggcagaat tggttgaaaa acgattactg
    11041 gaatttgagc gtgtaaaagc cagagaaaaa ttatcacaaa ccgaaaaaca actttcnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggat gggaacaata caactgaaaa
    11221 agaaattagg cgtacctgat agtcgtcccg ttgcagattt tttaccgacc ataagcataa
    11281 aggcaaaaga tttggccgct gaaatgacag ggttgaatgt gcaaaataaa gatttaaaag
    11341 gacaaagttt aatagaaaaa gaacatgttg acaataacct tgctgtccgt gaaatgttaa
    11401 cgaaaagagg aatatttccc gagaatcttc cacctgctga agatgttaaa aagttgcaac
    11461 gtaaattgga aggcgaagag aaaaaaatac tgacaaaaaa aagaagaaat aatgagtgat
    11521 gtggtaaata aattgtgggg cttttgccat acattaagac acgaaggaat tgattacaac
    11581 gattatatcg aacaactttc ttttcttctt ttcttaaaaa tggctgatga aaaagaagtc
    11641 catctgaacg acaaatacaa ttggaaagca ctgaaagaat taagcggtgg cgatttaatg
    11701 gaccactata ccgactcgtt aaaagaactg gctaaagaac cgggactact tggcgaaatc
    11761 tttacgcagg cacaaaaccg ttttaacaat ccggtaagtc tcaaaaaact gctcaacctg
    11821 attgatgaag atgaatggac ttcgatggac atagacgtga aaggtgctgc ctttgaagga
    11881 ctgctggaaa aggcagccag cgaagggaaa aaaggtgctg gacaatattt tacaccgcgt
    11941 cctttaatac aaagcatggt tcgcttgatg aagcccgatc caagaggaaa gaatacattt
    12001 accatttatg accccgcttg cggtacaggt ggttttttgg tttgtgctta tgaatggctt
    12061 ttacaggaat gtaaaggtgc ttttcctcgt gaagatgtga aacgaatcag agaaaacact
    12121 tattacggac aagaattggt tgcccgtcca agacgattgg ctttgatgaa tatgtatttg
    12181 catggcgtta ctcctaaaat aaaattaggc gataccattt ataataaccc aagtagcgac
    12241 cgttttgatg tgattttggc aaatccccca tttggcacaa agggagcaaa tcaaataccg
    12301 gagcgtgacg attttacggt agaaaccagc aacaaacaac tcaattttgt gcaacacatc
    12361 atgagtacgc tcaaaatagg cggacgtgcc gccatagttt tacccgacaa tgtattgttt
    12421 gaagataaag caggggaagt ttttgaaata ctgatgcagg aatgcaactt gcacaccatt
    12481 cttcgcttgc caagaggtac atttacacct tatgcacaag gcgtaaaagc aaatgttatt
    12541 ttctttcaga aaggattgcc taccgaaaaa gtctggattt acgacaaccg cagcaatata
    12601 gaaagcatta ccaaaaaaga ccgcccgcta acagccaaac atttcgaaga ttttgaaaaa
    12661 tgctatggct ctgaccccaa tggcaacagc ccacgaactg atcaaggaga aacagggcgt
    12721 ttccgttgct ttaccattga tgaaattaaa aaacgtaatt acaaacttga tatcacatgg
    12781 ctaaaagacg aaagcattga agatgccgac agcctgcccg aacccaacga ccttgctacc
    12841 gaagccattg ccgaactgga agctgtagtg gatgacttaa aagaaataat tagtttatta
    12901 gaaaaagaat aatgaaaggg tggatgtata ttttagaatg cagcaacggc atgtattata
    12961 ccggcagcac taataattta gaattacgat tgcaacaaca tcaaaatgga gaaggtgcta
    13021 attttactaa aaagcattta cctgttaagt tagtttattt tgaagaattt gcaagagttg
    13081 atgaagcttt ttatagagag aaacaaattc aaggctggag caggaagaaa aaggaagcat
    13141 tgatcaataa tcaatttgag aaacttcatg attttgctga atgtaaaaat gagacacatt
    13201 ttttgaatgt acccttcgac tccgctcagg gtac
//
LOCUS       DAOTAX010000054        13171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3964_length_13171_cov_31.443352, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000054 DAOTAX010000000
VERSION     DAOTAX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_13171_cov_31.443352"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLM04_06450"
     CDS             complement(<1..802)
                     /locus_tag="PLM04_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02751.1"
                     /translation="MNKKLSLGTRFVLLSIIVFFVFIALTGLIYRYTAIGIEQNIPVL
                     TLLAGSYFFVQLILFLVIFKSHLPKWNFSWDNILTVSLFFLLISTVLSVNVSITATVL
                     FVVLAVLFAIVKKKHFQPHPLFYFMGAYYLFQYVGLLWSIDTAYGWRFVGRGLSFIVL
                     PLAFGFYSVSEEKKNLLLRIFFRFLEVYLLMVIVAYFYQVEFHKVDFGIGFHLKKNYF
                     CTPVPPGVDYYLLLGWAGYNHPTFVSFILTLMFGIGFYLWRNDNSPQTV"
     gene            complement(811..2304)
                     /locus_tag="PLM04_06455"
     CDS             complement(811..2304)
                     /locus_tag="PLM04_06455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02752.1"
                     /translation="MENPLVSVIIPVYNMEAFLAEAIDSVLASTYPNFEIIVVDDGST
                     DQSPVIAEHYSERDTRIRFYRQSNRGVAAARNLALQEARGEFVLPVDADDKISEIFLE
                     TAAKVLVQLPQVKIVTCEYEYFGEKQGKGKYRPYHRKLIARKNVINNCSMYRRRDALK
                     VGGYCEEIIGREDWEFWISLLKNGGEVEHLSETGFYYRIRSNSKRITDRKLKKHIIDV
                     LNRRHKAFFYRELGGKLHYRRSLSRLLNFFYHCYKPETFFVHPDFKEEEEFFYSLPDI
                     FDNQGETIHSGRNIIKIFNAGNRKWVVKSFQSPNVVNRFIYGIFRKSKARRSYEYALK
                     LLSLGIATPQPIGYYEQKIFFLFNKSFYVSESSECNHRFEELIRDVNFPDREKILKAV
                     ARFTAELHEKGVLHSDYSAGNILFSQKNDDIHIELIDLNRLKFGKISKTAGCRNFERL
                     NLEPEALKTMAEEYAVARGFEKQECVELVQRYRWKKHKNISSLQPKN"
     gene            complement(2309..3031)
                     /locus_tag="PLM04_06460"
     CDS             complement(2309..3031)
                     /locus_tag="PLM04_06460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02753.1"
                     /translation="MNQQALHIITPVKDSLETTLQTIGSIMDSDFHSDFQYTVYNDFS
                     SDETTEKLEIVSAEKGFRLVNLKDITTHPSPNYLLVLQLAQQKALSEHAHLLVVESDV
                     IVKKNTIQILFDAAQKLENPGLIAAVTHDEEGNINFPYLYAKNFDSGTVQTSKRLSFC
                     CTLLSNSFLEAFDFKNLNPEKSWYDVFISHKAVELGFNNYLLTYLPVLHLPHSSRPWK
                     QLKYTHPLKYYWLKYTKGRDKI"
     gene            complement(3028..4512)
                     /locus_tag="PLM04_06465"
     CDS             complement(3028..4512)
                     /locus_tag="PLM04_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HON02754.1"
                     /translation="MKRLAKDTAIYGLSSIIGKFLNWCLVPLYTYVLESSADYGIVTN
                     LYAWTALLLVILTYGMETGFFRFANKNRDDAPKVYGTTMISVGFTSLLFATICVIFSQ
                     PIANGMGYPKYPEFIAMLAIVVSMDAFDAIPFAYLRYKNRPIKFAALKLFMIFTNIFF
                     NIFFLVICPWLQKKAPGLISWFYNPHYGVGYIFVANLISTAAVTLALLPDMLEAKFRL
                     DMALLKKMLQYSLPLLLLGIAGIIDQTIDKIIFPFLFEDKAYAAAQLGIYGACFKISM
                     VMMMFTQAFRYAYEPFIFAQYKDKNSTAAYADAMKYFIIFSLMIFLGMVFYLDIFKFI
                     IRSDYWAGLRVVPIVLFSYLFQGVFFNLSLWYKLTDKTMYGAWISIVGSLITIAINII
                     FVPVFSYMASAWASFACYFAMMLISYFLGQKYMPIKYDLKTIGLYTIVTLALYCISLL
                     IATPYLWLNLLLKTILFVLFVALLIKRDFPLQSIPVINRYLKKS"
     gene            complement(4580..5593)
                     /gene="ruvB"
                     /locus_tag="PLM04_06470"
     CDS             complement(4580..5593)
                     /gene="ruvB"
                     /locus_tag="PLM04_06470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HON02755.1"
                     /translation="MTENFDIRSNNIEKDFENALRPLTFSDFEGQNKIVENLKVFVQA
                     ARMRSESLDHVLLHGPPGLGKTTLSNIIAHELNVGIKITSGPVLDKPGDLAGLLTSLE
                     PNDILFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLELNPFTLIGATTR
                     SGLLTSPLRARFGINCHFEYYDTEVITHIIKRSAYLLNVQIEERAAIEIGSRSRGTPR
                     VANALLRRVRDFAQVKGSGKIDLEIACYALEALNIDKYGLDEIDNKILTTIIDKFNGG
                     PVGLSTIATALGEDAGTLEEVYEPFLIMEGFLKRTPRGREVTELAYRHLGKSKMGMMD
                     TLF"
     gene            complement(5657..6568)
                     /locus_tag="PLM04_06475"
     CDS             complement(5657..6568)
                     /locus_tag="PLM04_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HON02756.1"
                     /translation="MSINITKLSKNFGQQKVLDDVCFDIDKNGVVGFLGPNGAGKTTT
                     MRIIAGALPYETGSVKVCGHEVKDAPLKIKSLIGYLPEQNPLYEEMYVKEYLLFIADV
                     YKLGQYRNKKVDEMIDKVGLRAEFRKKIGQLSKGYRQRVGLAQALIHEPKVLILDEPT
                     TGLDPNQLEEIRTLILELGKDRTVLLSTHIMQEVKALCNRVIIINKGKIAADFEELSS
                     IHSLNRDIFQVEVEFFKKPAAKDIENIPGVLKVNFLDETRLLIVASEDIRSEIFDFSV
                     KQGNKILLMKLVEQEIEQVFRSLTAES"
     gene            complement(6716..7384)
                     /gene="pssA"
                     /locus_tag="PLM04_06480"
     CDS             complement(6716..7384)
                     /gene="pssA"
                     /locus_tag="PLM04_06480"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HON02757.1"
                     /translation="MKKHIPNFLTCLNLFSGCIGVYYAFQGNYFAVLVFILIAAFFDF
                     IDGLAARLLQAYSPMGKELDSLADVVSFGLAPGAVCFSLLSQSSLPFWVAFAGFVITL
                     FSALRLAKFNIDERQGSSFLGLATPANAIFWVGLGYSFSEFVTANSWLILILIVIFSA
                     LLVSEIPMFSLKFKNLKWEENELQYIFLMLCVLFLFVFQLNAFVPIIIAYLFLAVVKV
                     VFKI"
     gene            complement(7516..7962)
                     /gene="dtd"
                     /locus_tag="PLM04_06485"
     CDS             complement(7516..7962)
                     /gene="dtd"
                     /locus_tag="PLM04_06485"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HON02758.1"
                     /translation="MRVLIQRVAEASVEIDRQIISSIGKGLLVFVGIESTDTLQDAEW
                     LAGKTAALRIFDDEKGVMNLSVTDIDGEVLVVSQFTLCAETRKGNRPSYIRAARHETA
                     IPLYESFCKIMEQKTGKEVKTGVFAADMKVKLVNDGPVTIWIDSKV"
     gene            complement(8010..9110)
                     /gene="ychF"
                     /locus_tag="PLM04_06490"
     CDS             complement(8010..9110)
                     /gene="ychF"
                     /locus_tag="PLM04_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HON02759.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVT
                     QVPDERINILARLANPQRLVPATVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFEDENVAHVNGVIDPVRDKEIVDAELQLKDLETVESRINKVQKQAQTGGDKQAK
                     IIYEILQEYRSALLQGKSARTVVLDKESKKLVKDLFLLTDKPVMYICNVDEASATSGN
                     AYVEAVREAVKEENAEILVVAAATEAEIAELESYEERQMFLEELGLKESGVSRLIQSA
                     YRLLNLETFFTVGPLEVRAWTYEKGLKAPQCAGVIHTDFEKGFIRAEVIKYEDFVALG
                     SETACREAGKIYIEGRDYVVQDGDIMHFRFNV"
     gene            complement(9332..9898)
                     /gene="purN"
                     /locus_tag="PLM04_06495"
     CDS             complement(9332..9898)
                     /gene="purN"
                     /locus_tag="PLM04_06495"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HON02760.1"
                     /translation="MSKNIALFASGSGSNAENIIRYFTGNSDFSFPLIISNNEKAFVH
                     ERAKKLGVPSLTFPREDFIDGGKILNLLQEKNIDAIVLSGFLLKIPDSIIEAYPQKIV
                     NIHPALLPKFGGKGMYGMNVHRAVLEAGEKETGITIHYVNSNYDEGDIIFQAKCPVLP
                     DDTPESVAQRVHALEYEFFPRIIAEIWK"
     gene            10050..10286
                     /locus_tag="PLM04_06500"
     CDS             10050..10286
                     /locus_tag="PLM04_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HON02761.1"
                     /translation="MSEVEEKVKTIIADKLGVDEAEITPTASFTNDLGADSLDTVELI
                     MEFEKEFGISIPDDQAEKITTVGDAVSYIENAKK"
     gene            10302..11564
                     /gene="fabF"
                     /locus_tag="PLM04_06505"
     CDS             10302..11564
                     /gene="fabF"
                     /locus_tag="PLM04_06505"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HON02762.1"
                     /translation="MELKRVVVTGLGAITPLGSSVPKLWENILNGVSGAGPITHFDAS
                     QFKTQFACEVKDFDPFQYFEKKEIRKVDPYAQYAIAAAKQAIENCGINLETVDKDEVG
                     VIFSSGIGGITTFEQEVSNYFLNKEMGPKFNPFFIPKMISDIAAGQISILYGFKGPNY
                     ATTSACASSTHSIIDAFNYIRLGKAKMFVVGGAEAAITAAGVGGFNSMMALSTRNDDP
                     AGASRPFSKSRDGFVIGEGAGCMILEELDHALARGANILCEVAGGGMSADAYHLTASH
                     PEGLGAILAMKSALKDAGMQVEDIDYINVHGTSTPVGDTSEAKAIENVFGEQAYQLNI
                     SSTKSMTGHLLGAAGAVEAIICTLAIINDVVPPTINFTEGDEDPDINYRLNFTFNKPQ
                     KRTINAALSNNFGFGGHNASVILKKFTK"
     gene            11569..12336
                     /gene="rnc"
                     /locus_tag="PLM04_06510"
     CDS             11569..12336
                     /gene="rnc"
                     /locus_tag="PLM04_06510"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HON02763.1"
                     /translation="MKNFFHLNGLFSKYRKEPYSLFRKLLGFYPRDINYYLLALTHRS
                     LSMTNHDGKVVSNERLEFLGDAVLNAIVSDLFYRYYEQKDEGFLTNIRSKIVNRDSLN
                     HLALKLELDKIVKVSKNVNPQVSNDIYGNALEALIGAIYLDYGYNHCMKFVKTKLFEP
                     FIDLSKVEKFEMNYKSKLIEWCQKNHFQIDFEMSCKTHNENHQPIFLSRVLIEGRPVS
                     ESTGLNKKESQQKASRIAYQQIIEQKTFHFINEESKN"
     gene            12490..12690
                     /locus_tag="PLM04_06515"
     CDS             12490..12690
                     /locus_tag="PLM04_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HON02764.1"
                     /translation="MSTENIGINAGAVWKVLNEKGEMDVKALKKSAKLSEKDLNMALG
                     WLAREGKIEFNETEENLLVHLV"
     gene            12840..12912
                     /locus_tag="PLM04_06520"
     tRNA            12840..12912
                     /locus_tag="PLM04_06520"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12873..12875,aa:Gly,seq:gcc)
     gene            12938..13024
                     /locus_tag="PLM04_06525"
     tRNA            12938..13024
                     /locus_tag="PLM04_06525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12971..12973,aa:Leu,seq:taa)
BASE COUNT         4329 a         2799 c         2294 g         3749 t
ORIGIN      
        1 taactgtttg cggtgaattg tcgtttcgcc acaaataaaa cccgattcca aacatcagcg
       61 tcaaaatgaa tgaaacgaaa gtcggatgat tgtagccggc ccaacccaac aacaaatagt
      121 aatccactcc gggaggtaca ggtgtgcaga aataattctt tttcagatga aatccaattc
      181 caaaatcaac tttatgaaat tcaacctgat aaaaataagc cactattacc attaataaat
      241 aaacttccaa aaaccgaaag aaaatacgca gcaacaaatt tttcttttcc tcggagacag
      301 aataaaaacc aaaagccaaa ggtaaaacaa taaaagacag ccctcttccc acaaaacgcc
      361 aaccataagc agtatcaatc gaccacagaa gcccaacata ttgaaacaaa taataggctc
      421 ccataaaata aaaaagcgga tgaggttgaa agtgcttctt ttttactatc gcaaacagca
      481 ctgccaaaac aacaaaaagg acggtggccg taatcgaaac attaacactt aaaaccgtag
      541 aaatcaggag aaaaaacaac gaaacggtca gaatgttgtc ccatgaaaag ttccattttg
      601 gtaaatgact tttaaaaatg acaagaaaaa gtatcagttg aacaaaaaaa taactcccgg
      661 ccaataatgt taaaacagga atattttgct caatgccaat agccgtatat cgataaatta
      721 agccggtgag ggctataaat acaaaaaaaa caattatcga caaaagaaca aaacgagttc
      781 ccaaagaaag ttttttattc attccaatgt ctaattttta ggttgtaacg agctgatatt
      841 tttatgtttt ttccaacgat accgttgaac gagttctaca cattcctgct tttcaaaacc
      901 acgagccaca gcgtattctt cagccattgt tttcaaagct tcgggctcca gattcaatct
      961 ttcaaagttc ctgcagccgg ctgttttgga tatttttcca aatttcaacc gattcaaatc
     1021 aatcaattca atgtgaatat cgtcattctt ttgagaaaac aaaatgttcc ctgccgaata
     1081 atccgaatgt aaaactcctt tttcgtgtag ctcagccgta aaacgtgcaa cagctttcaa
     1141 tatcttttct cggtcgggaa aattcacgtc tcgaatcaat tcttcaaaac gatgattgca
     1201 ttcagagctc tccgacacat aaaaactttt attgaataga aaaaaaatct tttgctcata
     1261 atagccaatc ggttgtggag tggcaattcc aagcgagaga agtttcaatg catattcata
     1321 cgaacgtctc gctttcgatt ttctgaaaat accatatata aagcgattca ccacatttgg
     1381 cgattgaaac gatttcacaa cccatttcct gtttcccgca ttaaaaattt taatgatatt
     1441 tcttcccgaa tgaatcgttt ctccttgatt gtcaaaaata tccggcaacg aataaaaaaa
     1501 ttcttcttcc tctttgaaat cgggatgaac gaaaaaagtt tcgggcttgt aacaatgata
     1561 aaaaaaattc aacaagcgag atagtgagcg tctataatga agttttccac ctaattcgcg
     1621 ataaaaaaaa gctttatgtc ttcggtttaa aacgtcgata atgtgttttt tcagttttct
     1681 gtcggtaatt cttttagaat tactcctgat tcgatagtaa aatcctgttt cagaaagatg
     1741 ctccacttct cctccatttt tcagcaacga tatccaaaat tcccagtctt cacgaccaat
     1801 aatttcctca caataaccgc ccactttcaa agcatctctc ctgcgataca tcgaacaatt
     1861 attaataaca ttcttccgag caatcaattt tctgtgataa ggtcgatatt tccctttacc
     1921 ctgtttctcg ccaaaatatt catattcaca cgtaacaatc tttacttgag gcaattgaac
     1981 aagtacttta gcagcagttt caagaaaaat ttcagaaatc ttatcatcgg catctaccgg
     2041 caaaacaaat tctccacgag cttcctgcaa ggcaagatta cgggctgctg ccactccacg
     2101 attggattga cggtagaatc ggattcgtgt atccctttca ctgtaatgtt ctgctatcac
     2161 tggcgactgg tctgtagaac catcgtcaac aacaattatt tcaaaatttg gataagttga
     2221 agccagtaca gaatctatcg cttctgccag aaacgcttcc atattgtaca ccggaataat
     2281 gacagagacc aaaggatttt ccattttctc aaattttatc tctacctttt gtatatttca
     2341 gccagtaata ttttaaagga tgcgtgtatt taagttgctt ccacggacga ctgctgtgag
     2401 gtaaatgcaa aacaggcaaa taggtaagta aataattgtt gaatcccaat tctacagctt
     2461 tgtgcgaaat aaacacatca taccatgact tttcgggatt caaatttttg aaatcgaatg
     2521 cttctaaaaa cgaattggag agcaatgtac agcaaaaact caaacgtttg gaagtctgca
     2581 ccgttccact gtcaaaattt ttggcataaa gatacggaaa attgatattt ccctcctcat
     2641 cgtgcgttac agcagcaatc agacccggat tttcaagttt ctgagcagca tcaaacaaaa
     2701 tttgaatggt gttttttttc actattacat ccgactccac aaccagcaaa tgtgcatgct
     2761 ccgacaaagc tttctgttgg gccagttgca ataccagcaa atagtttgga gagggatgcg
     2821 tggtaatatc tttcaaattt accaacctga atcccttttc ggcggaaaca atctccagtt
     2881 tttcggttgt ttcgtctgaa ctgaaatcat tataaacggt atattgaaaa tctgaatgaa
     2941 aatcggaatc catgatactt cctatggttt gcagggttgt ttccaacgaa tccttcacag
     3001 gcgtaataat atgcaaggct tgctgattca tgattttttc agataacgat tgattacagg
     3061 aattgactgt aacgggaaat cacgttttat taataaagct acgaaaagaa caaacaaaat
     3121 ggttttcaac agcaaattga gccacaaata gggcgttgca atcaaaagac tgatacagta
     3181 caaggcaaga gttacaatgg tgtagagacc gatagttttt aaatcatatt taatcggcat
     3241 atatttctgt cccaaaaagt aagatatgag catcatcgca aaataacagg caaacgaagc
     3301 ccacgccgaa gccatataac tgaaaacggg aacaaaaatg atattgatgg caattgtaat
     3361 caaacttccc acaatcgaaa tccaagctcc atacatggtt ttgtcggtaa gtttgtacca
     3421 aagcgaaaga ttaaaaaaca caccctgaaa caaataggaa aacaatacga tgggaacaac
     3481 acgcaaccct gcccagtaat cgcttcggat aatgaatttg aaaatatcca gataaaaaac
     3541 catacccaaa aaaatcatca aggaaaaaat gatgaaatat ttcatcgcgt cggcataggc
     3601 agctgtactg ttcttatcct tatattgcgc gaaaatgaaa ggttcataag cataacgaaa
     3661 agcctgagta aacatcatca tcaccatcga aattttgaaa caggcgccgt aaattcccaa
     3721 ttgagcagct gcataggctt tgtcttcgaa aagaaaggga aaaataattt tgtcgatggt
     3781 ttgatcgata attcctgcta ttcccaataa gagtaagggc aatgaatatt gaagcatttt
     3841 cttcaacaag gccatgtcca aacggaattt agcttccaac atatcgggca gcaaagccag
     3901 tgttacagca gcagtcgaaa tcagattggc tacaaaaata taacccactc cgtaatgtgg
     3961 attgtaaaac caactgatta aaccgggcgc tttcttttgc aaccacggac aaataaccag
     4021 aaaaaagata ttgaaaaaaa tgttggtaaa aatcatgaaa agctttaatg cagcaaattt
     4081 tatgggacga tttttatagc gcaaataagc aaacggaatg gcatcgaaag catccataga
     4141 aaccactatg gccagcatag ctataaattc gggatattta ggataaccca ttccattggc
     4201 aatgggttgc gaaaaaatca cacaaatggt tgcaaaaagt aaggaagtga accccaccga
     4261 aatcatggtg gtaccataaa ctttgggtgc atcatcccga tttttattgg caaaacggaa
     4321 aaatcccgtt tccattccgt aggtgagtat caccagcaac agagccgtcc aagcatacag
     4381 gttggtaact attccataat cagccgaact ttccaacacg taggtgtaca acggcaccaa
     4441 acaccagttg agaaatttcc ctatgatgct gctcaatccg taaatggctg tatcttttgc
     4501 cagccttttc atctgctttt ccggcatatt ctcttttaaa tatattttac aatggctcgg
     4561 ttgaaactgc taaaattttt caaaaaagtg tatccatcat tcccattttc gacttcccca
     4621 agtgtctgta tgccaattcg gtaacttctc ttcctcgtgg cgtgcgtttc agaaagcctt
     4681 ccatgatcag aaatggttca tatacttctt ccaacgtgcc ggcatcctcg ccaagtgctg
     4741 tagcaatagt agacaaccca acagggccac cattaaattt gtcgataatg gtagttaaaa
     4801 ttttattgtc tatttcgtcc aatccatatt tgtcgatatt cagagcttcg agcgcataac
     4861 aggcaatttc caaatcgatt ttgccgcttc ccttaacctg tgcaaaatcg cgtacacgcc
     4921 gcagcaaagc atttgccacg cgaggtgttc cccgacttcg actgccaatt tctatggctg
     4981 ccctctcttc aatttgcaca ttcaacaaat aagcagaacg tttaataata tgcgtaatca
     5041 cttctgtatc gtaatactcg aaatgacaat tgataccaaa tctggctctt aagggagaag
     5101 tcaataatcc gctgcgagta gttgctccaa tcagtgtaaa aggattcagc tccaactgaa
     5161 tggaacgcgc acttggacct ttgtcgatca taatatctat ccggtaatct tccatagccg
     5221 aatataaata ctcttccact accggactca gtctgtgaat ttcgtcaata aacaaaatat
     5281 cgttcggttc caggctggtg agcaaaccgg ccaaatcgcc aggtttatcg agtacaggcc
     5341 ccgaagtaat tttaatgcca acattcagtt cgtgagcaat gatattggac aaagtggttt
     5401 tccccaatcc cggaggccca tgcaaaagca cgtgatcaag cgattcggaa cgcatgcgag
     5461 ccgcttgaac aaaaactttc agattttcta caattttatt ttgtccttca aaatctgaaa
     5521 acgtcagcgg acgcaatgca ttttcaaaat ccttttctat attgttgctc cttatgtcaa
     5581 aattctctgt catgtaaaac tccaacaaaa aaaattgtat cctgaattat ttccgtgcca
     5641 aaattttttt ccaacattaa ctttctgcgg tcaaactgcg aaagacctgc tcaatttcct
     5701 gttccaccaa tttcatcagc agaattttat ttccctgttt cacggaaaaa tcaaaaatct
     5761 cactgcgaat atcttccgaa gccacaatca acagacgggt ttcatccaaa aaattcactt
     5821 tcaatacccc aggaatattt tcaatatctt ttgctgccgg ttttttaaaa aattctactt
     5881 caacctgaaa aatatcccga tttaaagagt gaatggacga aagttcctca aaatctgcag
     5941 caatttttcc tttatttata atgattactc tgttgcacaa agctttaact tcctgcataa
     6001 tgtgagtact gagtaacaca gttcggtctt ttcccaactc cagaattaaa gttctaattt
     6061 cttccaactg attgggatcc aagccggttg taggttcatc caaaatcaaa actttcggct
     6121 catgaatcaa agcctgtgcc aacccaaccc gctgacgata tcctttggaa agctgtccga
     6181 tttttttacg gaattcagca cgaagaccta ccttatcaat catctcatcc acttttttgt
     6241 tcctgtattg tcccaatttg taaacatccg caataaaaag cagatattct tttacataca
     6301 tttcctcgta caatggattt tgttcgggca aataaccaat cagcgatttg attttcaaag
     6361 gcgcgtcctt cacttcatga ccacaaactt ttacactgcc cgtttcgtaa ggcaaagctc
     6421 ctgcaataat tctcatcgtg gttgtttttc cggcgccatt tggccccaaa aaaccaacta
     6481 ctccattttt gtcgatgtca aaacagacat cgtccaaaac cttttgttgt ccgaagtttt
     6541 ttgaaagttt tgttatattg atggacacac gtttcgtgtt ttgtaaaaag ttatattcaa
     6601 aaatttcgac tgcaaaagta ataaatcctt accgtataac gaaatgaaag agaaagcgtt
     6661 ttaaacaaga aaaaagtcaa cttcctattc ggacagaacg aaacggcaga aaaaatcaaa
     6721 ttttgaaaac aactttaaca actgccaaaa aaagataggc aataataatg ggaacaaaag
     6781 cattcagttg aaaaacaaaa agaaacaaaa cacacaacat caagaaaata tattgcaatt
     6841 cattttcttc ccatttcagg tttttgaatt tcaaagaaaa catggggatt tccgatacca
     6901 gcagagcact gaaaattaca atcaaaatca aaatcagcca actgtttgcc gttacgaatt
     6961 cggaaaaaga atagcccaat ccaacccaaa aaatggcatt tgctggagtt gccaaaccca
     7021 ggaatgagga accttgccgc tcatcgatgt tgaatttggc cagtcgcaga gccgaaaaca
     7081 aagtaatgac aaatccggca aacgctaccc aaaacggcaa ggagctttga gagagcaaac
     7141 taaaacatac cgctccgggt gccaaaccaa aactcacaac atcggccaaa gaatcgagct
     7201 cttttcccat aggcgaataa gcctgcaaca ggcgagcagc caatccatca ataaaatcga
     7261 aaaaagcagc aatcaaaatg aaaaccaaaa cagcaaaata atttccctga aaagcataat
     7321 aaactccaat acagccagaa aataaattca agcaggtcag aaaattcgga atatgttttt
     7381 tcatactttg tcctaattta tggtaaatac ttttttaaaa ccactgaaat caaaaaaaat
     7441 cgaaaacttt ggaacaacaa aaaatgggac aaacatttct tttttgattt tttctgcaat
     7501 gaaacagcaa aattatcaaa ctttactgtc tatccaaata gttaccggac catcatttac
     7561 cagtttaact ttcatatcgg ctgcaaaaac gccggtttta acctctttac cggttttttg
     7621 ttccattatt ttacaaaaac tttcgtaaag cggaatggct gtttcatgcc gtgcagcccg
     7681 aatataagaa ggtcggttcc ccttcctggt ttctgcacaa agtgtaaact gactcaccac
     7741 caacacttct ccatcgatat ccgtaacgga aagattcata actccttttt cgtcgtcaaa
     7801 aatacgtaat gctgccgttt taccggccaa ccattcagca tcctgcaaag tatcggtgct
     7861 ttctatacca acgaagacca acaatccttt tcctatggaa gatattattt gtcgatcaat
     7921 ttctaccgaa gcttctgcta ctcgctgaat taaaacgcgc atgacaaatt ttgcttttta
     7981 aggcaatttg aagcaaaaat agattaaact caaacattga atcggaaatg cataatatcg
     8041 ccatcctgca ccacataatc cctgccttcg atataaatct ttcctgcttc tctgcaagcc
     8101 gtttccgagc ctaaggccac aaaatcctca tacttgatta cttcggcacg aataaagcct
     8161 ttttcaaaat cggtatgaat tacgccggca cattgcggag ctttcaatcc tttttcgtaa
     8221 gtccatgccc gaacttccag tggcccgaca gtaaaaaatg tttccaaatt gagcaaacga
     8281 taagccgatt gaatcaagcg ggatactccc gattccttta aaccaagttc ctccaaaaac
     8341 atctgacgct cttcatacga ttccagctcg gcaatttcgg cttcagttgc ggcagccacc
     8401 accaatattt cggcattctc ttcctttact gcttctctca ctgcctccac ataagcattt
     8461 ccgcttgttg cagaagcttc gtccacatta cagatataca ttacaggctt gtcggtcaat
     8521 aaaaaaagat ctttgaccaa ctttttagat tctttgtcca acactacggt acgtgccgat
     8581 tttccctgta acaaagctga ccgatattcc tgcaaaattt cataaattat tttcgcctgc
     8641 ttgtcgccac cggtttgggc ctgtttctgc actttattta ttcggctttc tactgtttcc
     8701 aaatctttca actgcaactc cgcatctact atttccttgt cgcgaaccgg gtcaataact
     8761 ccattgacgt gggccacatt ttcatcttca aaacaccgca atacatgcag aatggcatct
     8821 gtttcacgaa tattggccaa aaatttgttt cccaagccct ctcctttact cgcacctttt
     8881 accagtccgg caatatccac aatttccacc gtagccggaa ctaatctttg cggatttgcc
     8941 aatcgggcca agatattaat cctttcgtcg ggaacttgcg taacgccaac attgggttca
     9001 atggtacaaa aaggaaaatt ggccgcttga gcctttgcat tcgacagaca gttgaataat
     9061 gtggatttgc caacattcgg caaacctaca ataccacatt gaagtgccat ataattatcc
     9121 gatcaattaa attattcttc ttttttaaaa ctatttcttt gtacaaaagt acatgataat
     9181 tttcaattta cacaccgact atttaaaaac aaactttccc gattcataaa atcaaaccta
     9241 taaaactcat ttcagtaatc atgcaaatga atgaaaatga aaaataagct gaaacttctt
     9301 caggaacgat ctgaaaattg aacggagaaa tttatttcca tatttcggca atgattcggg
     9361 gaaaaaactc gtattcgaga gcatgtaccc tttgagcaac cgattcggga gtatcgtcgg
     9421 gcaacacggg acattttgcc tgaaaaataa tatcgccttc gtcgtaattt gaattgacat
     9481 agtgaatggt aatacccgtc tccttttctc ctgcttccaa cacggcacga tgcacattca
     9541 tgccatacat acctttccct ccaaactttg gcaacaaagc aggatgaata ttgacaatct
     9601 tttgcggata agcttcgata atcgaatcgg gaattttcag taaaaatccg gacaaaacaa
     9661 tcgcatcaat atttttctcc tgaagtaaat tcaaaatttt tcctccatcg ataaaatctt
     9721 cccgcggaaa agtaagcgaa ggaacaccca gtttttttgc ccgctcatga acaaaagctt
     9781 tctcgttgtt ggaaataatg agaggaaaag aaaaatcgga attgcctgta aaataacgaa
     9841 ttatattctc agcattggaa cctgaacccg aagcaaacaa ggcgatattt tttgacataa
     9901 ttggtgaaga aaaaatgctc aaaaacagct gatttgtgca aaaaaacata tttttttcta
     9961 aaaagtgatg attatttgaa gaaaatttca tttctttgca ccgcaaaatt ataaaacaat
    10021 ttttatttat aaacttaaaa cttaaaatta tgtcagaagt tgaagaaaaa gtaaagacta
    10081 ttattgcaga caaattgggt gttgatgaag cagaaattac acctacggca agctttacca
    10141 acgatcttgg cgctgattcg cttgatacgg ttgaattgat tatggaattt gaaaaagaat
    10201 tcggtatttc tattcctgac gatcaagctg aaaaaatcac cactgttggc gatgctgtaa
    10261 gctatatcga aaatgctaaa aaataacatt ccttatttgt tatggaatta aaaagagtag
    10321 ttgttacagg gctgggagcc attactccat tgggttcatc tgttcccaaa ctgtgggaaa
    10381 atatcctcaa tggggtaagt ggagctggtc ccatcactca cttcgatgct tctcaattta
    10441 aaacgcaatt tgcttgcgaa gtcaaggatt ttgatccttt tcagtatttc gaaaaaaagg
    10501 aaatcagaaa agtcgatcct tatgctcagt atgctatcgc agctgccaag caagctattg
    10561 aaaactgcgg aataaatttg gaaactgtcg acaaggatga agttggcgtc atcttttcgt
    10621 cgggaattgg cggaatcact acttttgagc aggaagtttc aaactatttt ctcaacaagg
    10681 aaatgggccc gaaatttaac ccgtttttta tcccgaagat gatttcagat attgcagcag
    10741 gtcagatttc cattctttat ggttttaaag gcccaaatta tgctacaacc tcagcttgtg
    10801 cttcatctac tcattcaatt atcgatgcct tcaattacat ccgtttaggc aaagcaaaaa
    10861 tgtttgttgt tggaggagcc gaagcagcca tcacagctgc cggagttggc ggattcaatt
    10921 ccatgatggc actatctacc cgaaatgatg atcctgcagg agcttcacgc cctttcagca
    10981 aaagcagaga tggttttgtc attggcgaag gagccggttg catgattctg gaagaattgg
    11041 atcatgcttt ggctcgtgga gcaaatatac tttgtgaggt ggcaggcggc ggcatgtcgg
    11101 ctgatgctta ccatttgaca gcttcacacc ctgaaggact tggcgctatc ctcgccatga
    11161 agagtgcact caaagatgca ggaatgcaag ttgaagacat cgactacatt aatgttcatg
    11221 gtacttctac gcccgttggc gacacatccg aagcaaaagc cattgaaaac gtttttggag
    11281 aacaggcata tcaactgaat atcagttcaa ccaaatcgat gacaggccat ttgctgggcg
    11341 ctgccggtgc tgttgaagcc attatctgta cgcttgccat tattaatgat gtggttccgc
    11401 ccaccattaa tttcacggaa ggcgatgaag accccgatat taattaccgg ttaaacttta
    11461 ccttcaataa gccacagaaa agaaccatta atgcagcttt atccaacaac tttggttttg
    11521 gtggacataa tgcaagtgtt atattaaaaa aattcaccaa ataagttcgt gaaaaatttc
    11581 tttcatttga atgggctctt ttccaaatac agaaaagagc cttattcttt gttccgaaaa
    11641 ctattgggct tttatccgcg cgatatcaac tattacctgc tggctctgac acatcgctcc
    11701 ttgtcaatga cgaaccatga tgggaaagtt gtgagcaacg aaagacttga gtttttgggt
    11761 gatgccgtat tgaacgctat cgtttccgat ttattttacc ggtattatga gcaaaaagat
    11821 gaggggtttc tcaccaatat tcgttccaaa attgtcaatc gcgattcgtt gaatcacttg
    11881 gcactgaaac ttgaattgga caaaattgtg aaagtttcca aaaatgtgaa tccgcaagtc
    11941 agcaacgata tttacggaaa tgctttggaa gctttaattg gcgcaattta tctcgattac
    12001 ggatacaacc actgtatgaa gtttgtcaaa acaaaacttt ttgagccttt tatcgatttg
    12061 tcaaaagtag aaaaattcga gatgaactat aaatcgaaat taattgaatg gtgtcagaaa
    12121 aatcacttcc aaatcgactt cgagatgtcc tgcaaaactc acaacgaaaa ccatcaacca
    12181 atttttctgt cgcgtgtact cattgaagga aggcctgttt ccgaatcaac gggactgaac
    12241 aagaaagaat ctcaacaaaa agcctctcgc atcgcctatc aacaaattat cgagcagaaa
    12301 acatttcatt ttataaacga agaatcaaaa aattagtctg cctcaactca gcaaaactta
    12361 agctgaaaaa gctactaaaa ggaaattcct tttcagcaac agattaaatt aattttttgg
    12421 acaattgaaa acttttaact aattttgtag gtgatataaa ttttttgtat aactaaaaac
    12481 caaaccgcta tgtcaacaga aaacatcgga attaatgccg gagccgtatg gaaagtattg
    12541 aacgaaaaag gagaaatgga cgttaaagcg cttaaaaaaa gcgccaaact ctctgaaaaa
    12601 gatttgaata tggcactagg ctggcttgcc cgagaaggaa aaattgaatt taacgaaaca
    12661 gaggaaaatt tattggttca cctcgtttga gagagtgaaa tataaaaaag gcggaatgga
    12721 aatttcgcct tttttcaaat tttaaggaat cctgcttcac ttttcaagcc aaaaaatttc
    12781 gtaaagcgaa aaaatgcttg tatatttgca cccgctttac gcatgaaaat gttagatatg
    12841 cgaaaatagc tcagttggta gagcacgacc ttgccaaggt cggggtcgcg ggttcgagtc
    12901 ccgtttttcg ctcattgaaa aagtctttta cttttttgcc cggatggtgg aatggtagac
    12961 acgaaggact taaaatcctt tggccccaaa ggctgtgcgg gttcaagtcc cgcttcgggt
    13021 accaacaaac cccttactca tctttaagat ttgcaagggg tttgttattt ttttgcaaat
    13081 tcctaatgaa tttgcttggg ctcacctgca aaaaatatgt gtttgtagaa aaagaaaagt
    13141 atctttgtga aaaaatactt tttatatgtc c
//
LOCUS       DAOTAX010000055        13167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_3965_length_13167_cov_29.476586, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000055 DAOTAX010000000
VERSION     DAOTAX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_13167_cov_29.476586"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PLM04_06530"
     CDS             <1..161
                     /locus_tag="PLM04_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HON02765.1"
                     /translation="VPAMIGAMMFLQGKWKKPGVFNVEEFDPDPFMEQLNKQGLPWHE
                     LVNVDLEL"
     gene            complement(227..427)
                     /locus_tag="PLM04_06535"
     CDS             complement(227..427)
                     /locus_tag="PLM04_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02766.1"
                     /translation="MYLIIDMPMQKVTTSDNSADEVDSGQQKKTKVMCPQMETIERIL
                     QFAAAYRAEKFNEGQYVELLLN"
     gene            complement(518..1111)
                     /locus_tag="PLM04_06540"
     CDS             complement(518..1111)
                     /locus_tag="PLM04_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON02767.1"
                     /translation="MKDFNQFTDDELVKLYKEGNNQAFEILLKRYQSKVYTYIFLIVR
                     NRELTEDIFQDTFIKAIATIQQDRYVESGKFLAWINRIAHNLIIDYFRREKNENTFSV
                     EGINFDILNCTRFSEKSMEDVLSNEQVFVDVVHLVEKLPLSQQAVVKMRFFENLSFKE
                     IAEKTNVSINTALGRMRYALINLRKIASEKNISLEIK"
     gene            complement(1288..2472)
                     /locus_tag="PLM04_06545"
     CDS             complement(1288..2472)
                     /locus_tag="PLM04_06545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HON02768.1"
                     /translation="MHVVFLKPKKEESVRRFHPWVFSGAIQKIEGQPSEGDLVKVLDS
                     QGNFLGIGHFQQGSIMVRLLSFQEEPIDENFWKNRIAEAYQMRKALGLMIQNRNNCYR
                     LVHGEGDGLPGLIVDVYGNTAVMQAHSVGMHKIRNVLAKCVVECVPEIQYLYYKSEAT
                     LPFRNENAPEDGYLIGGDSEQFVVMENGLKFFVDWQHGQKTGFFLDQRENRVLLERYA
                     SDRIVLNMFCYTGGFSVYALRGNAKLVHSVDSSAKAIDLTHKNVALNFSDNRHQSFAE
                     DAFKYLQLYEKRAPFYDLIILDPPAFAKHRDAVPNALKGYRKLNRKAFEIIRPGGIIF
                     TFSCSQAITREQFRQAVFSAAAESKRNVRILHQLSQGTDHPINIYHPEGEYLKGLVLW
                     VE"
     gene            complement(2524..3228)
                     /locus_tag="PLM04_06550"
     CDS             complement(2524..3228)
                     /locus_tag="PLM04_06550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HON02769.1"
                     /translation="MYSPKISKEEINQLPVVSFPGEIVLVDKPEMVAPAIDELRKNLV
                     VGIDTETKPSFKSGTSNKVSLLQLSELERCFLFRLNLIDFPQELQDFLNDENIKKVGL
                     SLHDDFRLLNKRQNFHPANFVDIQNIAKSYGIMELSLQKIYAIIFGKKISKAQRLSDW
                     EKPQLTEKQLRYAATDAWASLQIYLQLMNEPQLTPKQIEKLVQENTPHKQPETSENAE
                     TTNSDLKPDAHSEKLS"
     gene            complement(3236..3877)
                     /locus_tag="PLM04_06555"
     CDS             complement(3236..3877)
                     /locus_tag="PLM04_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HON02770.1"
                     /translation="MSIDELPDHIVYSANVIEFVTVVAETCLFLEHTSEMGKDEFVEK
                     SLKLLPLLYLKASVLDIPEPVYENLPERFVTEEDYQFVREQIEQLLGNDDVYLEVFHP
                     DMAISDTPVAAFISENLSDIYQEIKDFAANYQTADVDIMSDALSECLTAFGEHWGQKL
                     LNAMRALHALKYNPQDDTEWEEMHQPDEKQLTRDSFLDFQHTAENDDVSPFLK"
     gene            4106..4345
                     /locus_tag="PLM04_06560"
     CDS             4106..4345
                     /locus_tag="PLM04_06560"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HON02771.1"
                     /translation="MEDKIVTLKNYDTIVKAMFDQEILRANHIECFINNEQLVELYPM
                     FADIDEGLKIVVFEKDLEKARKVLEEYHSSIEENV"
     gene            complement(4342..4860)
                     /gene="folK"
                     /locus_tag="PLM04_06565"
     CDS             complement(4342..4860)
                     /gene="folK"
                     /locus_tag="PLM04_06565"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HON02772.1"
                     /translation="MSLIFLGLGSNLGEKEKNLQDAVMMLKMEIGNLLSQSSFYISKP
                     FGFSSENDFLNAVVLMETHLSPCELLDKTREIEQEMGRTSKSCGQQYADRIIDIDILL
                     YDHLVIDQPTLKIPHPAIAERDFVFIPLLEIAPDLVDPLSGKKFSELVEAKKITSELK
                     NFVVGKKEEKFR"
     gene            complement(4900..5514)
                     /locus_tag="PLM04_06570"
     CDS             complement(4900..5514)
                     /locus_tag="PLM04_06570"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HON02773.1"
                     /translation="MNQYVLYLVCLVAGYLIGSVLFAELITKLVSNQNIRELGNGNPG
                     AYNVFRNVGKFWGVLTGLLDAVKALVPMWIAYQFFHITDTTALGCIGIGAVIGHGYPL
                     YYHFKGGRAASTLLGMYAYFIGIELLIALAVDVIVLFGFIKKEYGIWGPSLLIALSGL
                     LCLFFPHEMGVKILVWIGALITLFFNRDKLMEKKGKVTEEVSQQ"
     gene            complement(5527..6411)
                     /locus_tag="PLM04_06575"
     CDS             complement(5527..6411)
                     /locus_tag="PLM04_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HON02774.1"
                     /translation="MSKKIFIIVGDNLGLSHKQIDYDALEIVKFPVFVDGVEYRQSEE
                     HNAKWLISKYEKEKVVAKSSTLIHNEIAEIIERNHANYDVFIHVVMSSGLSAASFRVA
                     ENVRKQFENIVPVINVDTRQSINGVGSVLLGIIDLIKTHPDLSVEELAAACQQIVENT
                     FSWFVFPDLNYLYKGGRIGKAQSLMGSVLHIIPVVGMMGNEKDGLILAMGKGRTFRQV
                     NDLIIEQIRAKMQEKSVNRIKRAITLSGYGDKNQDAYAALLEKIKTLPVDDVVEGEGG
                     LVDAVYCGPGAYGVSILL"
     gene            complement(6423..8264)
                     /locus_tag="PLM04_06580"
     CDS             complement(6423..8264)
                     /locus_tag="PLM04_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HON02775.1"
                     /translation="MNTTDTNLVETTEVSFNVESINEIIENIHRYLNKHKEYINSLNV
                     FPVPDGDTGLNMVLTIQGAIVQMKNNGRTEKNSGEYLKDFAEQMLLNSRGCSGVILSL
                     FAQGFTQITANNDFSKENIYRAIENGYRNAYEGTENPREGTMLTIMRALKEKYAQLME
                     KEENPLVIIQQTIPYLKEVLDQTPEMLPVLKKAGVVDSGGAGFIILLEGIDKELSQLA
                     VNGISLSSLLRIGRTTRKLLRKRLSELRKSPFAPLILNIDFSRIQNSRLVETLQSARN
                     LLGNIHLNHNNGKTLNREKLIDELQEIDDSWNPEIKNKYCTEFVLESDVITSKDQLKE
                     KIAHYGDSLIIINSNNKYKVHIHTNKPNDLFDDVSKFGSLLFTKVDDMKKQHRNFLSE
                     DVLDYERDKSVFCIVSGKGFADILKNLGADDILCYGKNKPSVNQLVKGLNNLKAKNII
                     AASDDSDILMALKYAASLCKSNVYIVESDNPISLVSMLMNISKDYDINTIFDEAMNSI
                     HNIPFCGIARSSRNVTTENGAPVNKNDYFTVYKKKIILANKNLDELLFDTVRQLSTEG
                     SLITLYKGADMKKENSFIERLRSKFPDRDFEEYFGGQYKYSYYITFE"
     gene            8709..9377
                     /locus_tag="PLM04_06585"
     CDS             8709..9377
                     /locus_tag="PLM04_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02776.1"
                     /translation="MFIRKVKNRSGSQSVQVIQKFRGKYKVVKTIGCATTQHEIERLE
                     QLAREEMNRLSGNQTKLFGYESDERIEQVFSLLHNGSIHPVGPELIFGKIYDYIGFNS
                     IQEELFRHLVIAWLAFPLSKLKTADYLRRYQGKEIEINTVYRFLGKLSGKLKPEVEQI
                     DFAHTLEVLGGKINVVFYDLTTLYFETSEEDDLRKMMGFSKEDKLLDVIKSVLFLQKI
                     VVTN"
     gene            complement(9345..10196)
                     /locus_tag="PLM04_06590"
     CDS             complement(9345..10196)
                     /locus_tag="PLM04_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HON02777.1"
                     /translation="MKPHILIYNALFVFLAVSVNAQPKLIIRGDDAGSARSANLAIIQ
                     NYEKGILKTTEVMVPCPWFYEAAKLMQQHPGLDVGVHLTITSEWDGYKWKPLTHCPSI
                     CDSEGNFLANTWGGQSGNSLQYANINLTEVEAELRAQVETAIKLIPQISHISEHMVFG
                     SLSPELRNLVKKLASEYNLLFEDDLITQMGVRKMIVNQSEDYKMLRKNFIQSIDTLPA
                     EGIWIWIDHPAINGFETQGMKLADNVKNVANDRNNIYRLLNDRKVKRRISNKKIELIS
                     YDYFLKK"
     gene            complement(11334..12215)
                     /locus_tag="PLM04_06595"
     CDS             complement(11334..12215)
                     /locus_tag="PLM04_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HON02778.1"
                     /translation="MDKRIKYIIEKIRFKDAIVVSEEDRKTSFAKIKERIAANEKVNI
                     PFEQKQTTFTLTFLQIAAAVAFIVSISALGFNIYFNQEINISNNTATIKNIVLPDGSQ
                     LELAKNTSVSYKRNFSYKRNIKLNGQALFSVSKNQQRPFTVQTKLGEIRALGTQFSVK
                     AFENNDFCKTYLIEGSVSVSNSINEILLKPGQEALITSNNSAITVIEVRNPQHAMAWK
                     TGIYIFEDESLTNILNTIADAHNKKLSINSLNAINKHYSVKFIRGEDLSTMLEVLADI
                     CPMRFRITQNEIIIDEK"
     gene            complement(12547..13101)
                     /locus_tag="PLM04_06600"
     CDS             complement(12547..13101)
                     /locus_tag="PLM04_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HON02779.1"
                     /translation="MTTEDQILWKNIHEADDIEAFEELHARYFSALCNFTYSYLRNKQ
                     LCEEVVSDVFVALWQKRKELNHVRAIKSFMYTCAKNLSIDTIRKNKIRIQSDTSLYEI
                     EIPDADDHILSPLEQNEFKEHINIAIEQLPPQCKLIFKMLINDQLTYKEIAEVLNLSR
                     KTVEAQIAIAYKKITLHLKKIYHC"
BASE COUNT         4024 a         2521 c         2243 g         4379 t
ORIGIN      
        1 gcgttcctgc catgattgga gccatgatgt ttttgcaggg aaaatggaaa aaacccggcg
       61 ttttcaatgt ggaagaattt gatccggatc cttttatgga acaactcaac aagcagggtt
      121 tgccctggca tgaattggtg aatgtagatt tggaactgta agtttttcag aaaaaaagaa
      181 aaagatattt ataccaaaaa agagcgcttg tgggcgctct tttttgttaa ttcagcaata
      241 attctacata ctgtccttcg ttgaattttt ctgctctgta agcagctgca aactgtaaaa
      301 ttctttcaat cgtctccatt tgaggacaca ttacttttgt ctttttctgc tgaccggagt
      361 ccacctcatc agcactgttg tcggaagtag taactttttg cataggcatg tctattatta
      421 aatacatttt tatctctcaa ataataaacg atgacacttg ccaaaaaagt gtacggacac
      481 aaacaaaaat aaaaatattt ttttcgctta cctgaattta tttgatttcg agagagatat
      541 ttttttctga agctattttt cgcaagttga tcagcgcata acgcattctg cccaaagccg
      601 tattaatgct gacatttgtt ttttcggcta tttccttgaa actcaaattt tcgaaaaaac
      661 gcattttaac cacagcctgc tgagaaagag gaagtttttc gaccaaatga accacatcga
      721 caaaaacctg ttcattagac aacacatctt ccatactttt ttcggaaaaa cgtgtgcagt
      781 tcaaaatatc aaagttaatt ccttctaccg aaaaggtgtt ttcgttcttt tcgcgacgaa
      841 aatagtcgat aattaagtta tgggctattc gattaatcca tgccaggaat tttcccgatt
      901 cgacataacg atcctgttga atggtagcta tggccttgat aaaagtatcc tgaaaaatat
      961 cttcggtaag ttccctgttg cgtacaatca aaaaaatgta ggtatatact ttcgactgat
     1021 aacgtttcaa taaaatctca aaagcctgat tgttaccctc tttgtacaat tttaccaatt
     1081 catcatcggt aaattgattg aagtctttca ttattgctaa tttattggtt aagggtaaga
     1141 atcggcaata ggcattgaag atttagaggt tgactattaa aaaattttta gtacgcaaaa
     1201 ataataattg tattgatata aaaaaatttt ttacaacaaa atttttcaaa aaacgttaca
     1261 aattttgagt cattctattc tatttcccta ttctacccac aaaaccaagc ctttcagata
     1321 ttctccttcc ggatgataaa tattgatggg atgatctgtt ccctgcgaaa gttgatgcaa
     1381 aatacgcaca tttcgtttgc tttcggcagc tgcactgaac actgcctgac gaaactgttc
     1441 ccgtgtaatg gcttgtgaac aggaaaaagt aaaaattatt cctccgggcc gaatgatttc
     1501 aaaagctttt ctgttgagct ttctgtaacc tttcaatgca ttggggacgg catccctgtg
     1561 tttggcaaaa gccggaggat caagaataat taaatcgtaa aaaggtgcac gtttttcgta
     1621 aagttgcaaa tatttaaaag cgtcttcggc aaaagattga tgacgattat cggaaaaatt
     1681 gagcgctaca tttttatggg tcaaatctat ggctttcgcc gaactatcaa ccgaatgaac
     1741 caatttggca tttccacgca acgcataaac ggaaaaaccg cccgtgtagc aaaacatatt
     1801 caaaacaatt cgatcggaag cataacgttc cagtaaaacg cggttttcgc gttgatcgag
     1861 aaaaaacccc gttttttggc catgttgcca gtcgacaaaa aatttcagtc cgttttccat
     1921 gaccacaaac tgttcggaat ctcctccaat taagtagccg tcttcaggag cattttcatt
     1981 acgaaaaggc agggttgctt ccgatttgta ataaagatat tggatttcgg gaacgcattc
     2041 taccacacat ttcgctaaca cattcctgat tttgtgcata cccaccgagt gagcctgcat
     2101 gacagccgta ttgccatata catctacaat caatcccggc aaaccatctc cttctccatg
     2161 aacaagtcgg tagcagttgt tccggttttg gatcatcaat cccaacgctt tacgcatttg
     2221 ataagcttca gctattcggt ttttccagaa gttttcgtca attggctctt cctgaaacga
     2281 aagaagacga accataatgc tgccctgctg aaaatggcct attcctaaaa aattcccttg
     2341 actgtcgagt acttttacca aatcgccttc cgaaggctga ccttcaattt tttgaatagc
     2401 accggaaaat acccacggat gaaatcggcg gacactttct tcttttttgg gtttcagaaa
     2461 aacaacatgc atattaaatg ttggatttta aaaattgaat aattatcatt cgacaaaaca
     2521 aactcaggaa agcttttctg aatgagcatc gggcttaaga tccgaatttg tggtttcggc
     2581 attttcggat gtttcgggtt gcttgtgagg cgtgttttcc tgcaccagtt tctcaatttg
     2641 tttgggggtc aattgtggct cattcatcag ttgcagataa atctgtaaac ttgcccaagc
     2701 gtcggttgcg gcatagcgaa gctgtttttc cgtcaattgc ggtttttccc aatcgctgag
     2761 acgttgtgct ttggaaattt ttttcccgaa aataatggcg taaattttct gcaaactcaa
     2821 ctccataata ccgtaactct ttgctatatt ttgaatatct acaaaattag ccggatgaaa
     2881 attctgacgc ttgttcagca aacgaaaatc atcgtgcaat gacaagccga ctttcttgat
     2941 gttttcatca tttaaaaaat cctgcaattc ctgtggaaaa tcgattaaat ttagacgaaa
     3001 aaggaaacaa cgttccaact cggaaagttg aagcagggaa actttattgg aagtaccgct
     3061 tttgaaagaa ggttttgttt cggtgtcgat ccccacaacg agattttttc gaagttcgtc
     3121 gatagcgggc gctaccattt caggtttatc cacaagaaca atttctcccg gaaaactgac
     3181 tacgggtaac tgattgattt cctctttcga aatttttggt gaatacataa aaaatttatt
     3241 tcaaaaaagg acttacatcg tcgttctcgg cagtatgttg aaaatcgaga aacgaatcgc
     3301 gcgtaagttg tttttcatca ggttgatgca tttcttccca ttctgtatca tcctgcgggt
     3361 tgtatttcaa agcatgtaag gcacgcatgg cattcagcaa cttttgaccc caatgttcgc
     3421 caaaagccgt aagacattca gatagagcat cgctcattat atccacatca gcagtttgat
     3481 aattggcagc aaaatccttg atttcctgat aaatatcact cagattttca gaaataaaag
     3541 ctgcaacagg tgtatcgctt atggccatgt cgggatgaaa cacctcaagg tacacatcat
     3601 cgttgcccaa aagttgttcg atttgttcac gaacaaactg ataatcttcc tccgtaacaa
     3661 aacgttcagg aagattttca taaaccggtt cgggaatatc gagcacggaa gccttcagat
     3721 aaagcaacgg caatagtttc agcgattttt ccacaaactc atccttcccc atttccgaag
     3781 tatgctccag aaaaaggcag gtttcggcaa ctactgttac aaattcaata acattggcac
     3841 tgtaaacgat atgatcgggc aattcgtcaa tactcatgat aatttcaata aaacttattc
     3901 attgtagcaa tgaaaatttt taaggtacaa aattaagaaa attattttac ataaaccggt
     3961 gaaatctttc aggccccatc tcaaaaaaag atagtcaact ttcgaaaaat ttgataaaca
     4021 atttttatct ttaccttgtt ctaaaccaaa aaggacatag caaatgcata atttattttc
     4081 taccaatacc ccaaaacaaa gcagtatgga agacaaaatt gtaacactaa aaaattacga
     4141 cacaatagtg aaagccatgt tcgatcagga aattttacgt gccaaccaca tcgaatgctt
     4201 tatcaacaat gagcagttgg tggagcttta tccaatgttt gccgacattg atgaagggct
     4261 gaaaatagtt gtttttgaaa aagatttgga aaaagcacgg aaggtattgg aagaatatca
     4321 tagctcaatc gaagaaaatg tttaacgaaa tttctcttcc ttttttccga caacgaaatt
     4381 ttttaattcg cttgttattt tttttgcttc aaccaattct gaaaactttt ttcctgagag
     4441 cggatcaacg agatcgggag caatttccag caagggaatg aatacaaagt cgcgctccgc
     4501 aattgcggga tgcggaattt ttaaagtagg ttggtcgatg acaagatgat cataaagcaa
     4561 tatgtcaata tcaataatac ggtcagcata ttgttgcccg caactttttg aagttctacc
     4621 catctcctgc tcaatttccc gggttttgtc caacaattcg cagggagata aatgggtttc
     4681 catcagcact accgcattca aaaaatcgtt ttcggaagaa aaaccaaatg gtttggaaat
     4741 ataaaacgaa gattgggaca ataaattccc aatctccatt tttaacatca tcacagcatc
     4801 ctgcaaattt ttttcttttt ctccaagatt gcttcccaaa cccaaaaaaa tcagactcat
     4861 ttttaaaaag attactttct ttgcatttct caccaatgat tattgctgcg aaacttcctc
     4921 cgtaactttt ccctttttct ccatcagctt gtcccgatta aagaacaagg taataagtgc
     4981 gccaatccaa accagaattt tgacacccat ttcatgagga aaaaacaggc aaagtaatcc
     5041 actcagtgct atcagaaggg aaggtcccca aataccgtat tcttttttga taaaaccaaa
     5101 taacacaatt acatcaactg ccagggcaat aagtaattcg ataccgataa aataggcata
     5161 catacccagc aacgttgagg cggctcttcc tcctttaaaa tggtaataaa gcggatagcc
     5221 atgtccgatg acggcgccaa ttcctataca tcccagagcg gtagtatcgg taatatgaaa
     5281 aaattgataa gctatccaca tgggaacaag cgccttcact gcatccagca gtccggtcag
     5341 aacgccccaa aatttgccaa catttctgaa aacattgtaa gcgccgggat ttccattgcc
     5401 taattcacga atattttgat tcgaaaccag cttggtgata agctcggcaa ataatacgga
     5461 accaattaaa tatcccgcaa ccagacaaac caaatataaa acatactgat tcataatatg
     5521 taagctttac agaagaatag atactccata agcacctggt ccacaataaa ctgcatctac
     5581 caatccacct tcaccttcaa caacatcgtc aaccggcagc gttttaattt tttccagtag
     5641 agcagcataa gcatcctgat ttttatctcc gtaaccactc aatgtaatag cccgcttgat
     5701 acggttaacc gatttttcct gcattttggc ccggatttgt tctataatca aatcgttcac
     5761 ttgccgaaaa gtcctgcctt tccccatagc caaaatcaat ccatcctttt catttcccat
     5821 catgccgaca actggtataa tgtgcagaac actgcccatc aacgactgtg cttttccgat
     5881 gcgtccgcct ttatacaaat aattgagatc gggaaaaaca aaccagctga aagtattctc
     5941 cactatttgc tgacaggctg ctgccaattc ttccacactt aaatcgggat gggtttttat
     6001 caagtcgata attccgagta atacgctgcc gacaccattg atagactgac gggtatcaac
     6061 atttatgacc ggcacaatat tttcgaactg tttgcgaaca ttttctgcta cacgaaatga
     6121 agctgcagaa agcccactgc tcatcaccac atgaataaac acatcgtaat tggcatgatt
     6181 tctctcaata atttccgcaa tttcgttatg aatcagagta gaagattttg ccacaacttt
     6241 ttccttttca tatttcgaaa tcagccattt tgcattatgt tcctcacttt gccgatattc
     6301 cacaccatcc acaaaaactg ggaattttac aatttccaac gcatcatagt caatttgttt
     6361 gtgtgaaagt ccgagattgt ctccgacaat gataaaaatt ttcttgctca tagtttcaaa
     6421 ttttattcaa aggttatata ataagaatat ttgtattgtc caccaaaata ctcttcaaaa
     6481 tcccgatcgg gaaatttact cctcaaacgt tcaataaaag aattttcctt cttcatatct
     6541 gcgcctttgt acagggtaat taaactccct tccgttgaaa gttgccgaac agtatcgaaa
     6601 agaagttcat cgagattttt gttggcaaga ataattttct ttttatacac cgtaaaataa
     6661 tcgtttttat tcaccggagc accattttct gttgtaacat ttcgtgaaga gcgtgcaatg
     6721 ccacaaaaag gaatattgtg aatactgttc atggcttcat caaaaatggt gttgatgtcg
     6781 taatcttttg agatattcat caacatactg accaacgaaa tcggattatc cgattcaaca
     6841 atgtaaacat tcgacttgca aagcgacgcg gcatatttca atgccatcag aatatcgctg
     6901 tcgtctgaag ctgcaataat attttttgcc ttcaaattgt tcaatccttt caccagctga
     6961 ttgacggaag gtttgttttt gccataacac agtatgtcat cagcgcccaa gtttttcagg
     7021 atatcagcaa aacctttccc tgaaacaata cagaatacgc ttttgtctct ttcgtaatcc
     7081 aacacatctt cgctaaggaa atttcgatgc tgctttttca tatcgtccac tttggtaaaa
     7141 agcaaagaac caaatttgga aacatcatca aacaaatcat tgggtttatt ggtgtgaata
     7201 tgtactttgt atttattgtt gctgttgata ataatcagac tgtcgccgta atgagcaatt
     7261 ttttccttca gttgatcttt agaagtaatt acatcacttt cgagcacaaa ttcggtacag
     7321 tatttatttt ttatttcagg attccatgaa tcatcaattt cctgaagctc atcgattaat
     7381 ttttccctgt tcagggtttt tccgttgttg tgattcaaat gaatattccc taataaattt
     7441 ctggcgcttt gtagcgtttc aacaagtcgg ctattctgga tacgggaaaa atctatattc
     7501 aaaatcagtg gagcaaatgg actttttcgt aattccgaca atctctttct tagtagtttt
     7561 cgcgttgtgc gtccaattct aagcaagctg gataaagaaa tgccgtttac cgccagttga
     7621 ctgagttcct tatcgattcc ttccaacaaa ataataaaac cggcgccccc cgaatcgacc
     7681 actccggctt ttttcagtac gggaagcatt tcaggcgttt gatccaacac ttctttcaaa
     7741 taaggaatgg tctgctgaat gattacaagc ggattttctt ctttttccat gagttgagca
     7801 tatttttctt ttaaagccct catgatggta agcatggtac cttctcgagg attctccgtg
     7861 ccttcgtaag cattgcggta accattttca atagcccggt aaatattttc tttggaaaaa
     7921 tcgttgttgg cagtaatttg ggtaaatccc tgagcaaata acgataaaat aacgcctgaa
     7981 caaccacgac tgttgagcag catttgctcg gcaaaatctt tcaaatattc tccggaattt
     8041 ttctctgttc tgccattatt tttcatttgg acaatagcac cctgaatagt tagcaccata
     8101 ttcaatcccg tgtctccgtc gggaactggg aaaacgttca aactgttaat atactccttg
     8161 tgcttattaa ggtaacgatg aatattttca ataatttcat tgatggattc cacattgaag
     8221 cttacttcgg tggtttcaac taaattggta tcggttgtat tcatgatgat ttaaataatt
     8281 tacccagatt tttaaaaata ctttggttta aaatatgagt tccaaaagta taaaatttta
     8341 tttaaaaaac attcagaaaa caaacaactt aatctattga ttatttaaat aaaagatttt
     8401 caataaaata tgcaaactcg gacttaaaaa acggtaaaat aactttttgt ggttttttct
     8461 cagcaaaaag aatactaaca gtattttcct tacccgaaac aaggcaacaa tttcagtgtt
     8521 actgcatttt tgtcagggaa aaaggtgcta aaatcggctc gcctgtcaaa tatgcgtttt
     8581 aaaagtaaga aaaaatatct tggtaaaaaa tacttttcct gttttccgca ttttttgcaa
     8641 catccttata agtatttgaa tatcaataat aataatattt taattttggg tgttgcaaaa
     8701 ttattattat gtttatccga aaagtaaaaa atcgatctgg aagtcaatct gtacaagtga
     8761 tacagaagtt tcgaggaaag tataaagtag tgaaaaccat tggttgtgcc accacgcagc
     8821 acgaaattga aaggttggaa caattggcca gagaagaaat gaatcgttta agcggaaatc
     8881 aaacgaagct ttttggctac gaaagtgatg agaggattga acaagttttt tctctgcttc
     8941 acaatggcag tattcatcct gttggtccgg agttgatttt cggcaagatt tacgattata
     9001 ttggttttaa ttccattcaa gaggagcttt tcaggcattt ggttatagcc tggttagcgt
     9061 ttccattaag caagttaaaa acagctgatt accttcgtcg ctatcaaggg aaagaaatag
     9121 agataaacac ggtttatagg tttttgggca aattaagcgg gaaactcaaa ccagaagtgg
     9181 aacaaattga ttttgcccat acccttgaag ttttaggcgg caaaattaat gttgtgttct
     9241 atgacctgac tacactgtat ttcgaaacca gtgaggagga tgatttgcgc aagatgatgg
     9301 gtttcagtaa agaggataaa ttgctggatg taatcaaatc agtattattt cttcaaaaaa
     9361 tagtcgtaac taattaattc aatttttttg ttggaaatcc tgcgtttaac tttcctgtcg
     9421 ttcaacaatc tatagatatt atttctgtcg ttagcaacat tttttacatt atctgcaagc
     9481 ttcattccct gcgtttcgaa accatttatg gcaggatggt caatccatat ccatattcct
     9541 tcggcaggga gagtatcaat agactgtata aagttttttc tcaacatttt gtagtcttca
     9601 ctttgattta caatcatttt tcggacaccc atttgggtta tcagatcatc ttcaaaaagc
     9661 aggttgtatt cggatgcaag ctttttaacc agatttcgca actcaggtga taaacttcca
     9721 aaaaccatgt gttccgaaat gtgacttatt tgtggaatta gtttgattgc tgtttcaact
     9781 tgtgctctta actcagcttc cacttcggta agatttatgt tagcatattg aagcgaattt
     9841 ccggattgac caccccaggt attcgccaaa aagtttcctt cgctatcgca aattgaggga
     9901 cagtgagtca gaggtttcca tttataacca tcccattcgc ttgtaattgt cagatgaaca
     9961 cccacatcta gcccgggatg ttgttgcata agctttgctg cctcataaaa ccagggacaa
    10021 ggcaccatca cctctgttgt ttttaaaatc cctttttcgt agttttggat gattgccaga
    10081 tttgccgaac gtgcggaacc cgcatcgtcg cctcgtataa ttaattttgg ttgagcattt
    10141 acactaacgg ctaaaaagac aaatagtgca ttgtagataa gaatatgtgg tttcatcaga
    10201 atataattta ttttttaaag gtctgatgga accccaatgt ctgaatatta tatttattcg
    10261 tgtttgtgta tgcaatacat gtgggtttca tatcagaatt gatttttcta ttgcaaatgt
    10321 acgcgataaa ctgcaaacaa ttatgtattt atttatcatt tattaatact atttcgatgt
    10381 ctcataccaa gttgatatct aaaatattta gataaggaaa caaagtcata gatttcacat
    10441 aattaggttt gcatttaatt cgtttattcc ccggcaaccc acttccattg ttttcgctaa
    10501 agaatcaaat atcttgttgt gtaaatactt tactcttatt tcatcccaaa gatgttgtgt
    10561 tgaattaact tcaagactaa aagatggttg ctatatgaaa tgtatatttc cgggcactct
    10621 taaacctttt gctttgtgcc atcctgttcc atctatctgc atgataattt caaattcctt
    10681 aaattcattg gatatatttt ataaaaataa gctcatcatt tccgtatttg catgcggtaa
    10741 tattaaagaa ctaatgtggc ctaactttgg acatattcct gtataaacat aaaaaaactt
    10801 tctcttacta cttattttgc aaccagagga cgaacctgct tgggtgctca acaactcttg
    10861 aaatatttac tctttaaatc ttcattcatc ctgtgtcatg agaacgactt tacttttatc
    10921 aagatttttc tcttcaatca attgactcac tttattaccg aagtttgtta aaagcttctt
    10981 cttcctcctt gttctgtttg gggtgtacag gaagggggta caatttttct ccaaccattt
    11041 cttttaagca tcctgtaaac agtagttttg ttaactctct ttcctgtctt cttttcaaaa
    11101 ttttctttga tttacatggc agttattatt tgtccttccc tggctttctc caaataacta
    11161 caaataattc ctcctcttcc ttacttaagt atgaatgcct ccttccacct ttaccttgag
    11221 tttctataga cgctattcca tattcaatga atggaaagtt gggttaaatc agctgcttaa
    11281 tctaaaaaaa aaagtgtctt tttaggacac cttctttttt tacttttaat taactatttt
    11341 tcgtcgatta tgatttcatt ttgagtaatt ctaaatctca ttgggcatat atcagccaag
    11401 acttcgagca ttgtggataa atcttcacca cgaatgaatt ttactgaata atgtttgttg
    11461 atagcattta agctgttgat tgatagtttt ttgttgtgtg catcagcaat agtattaagt
    11521 atgtttgtca acgattcgtc ttcgaaaata taaattccag ttttccatgc catagcatgt
    11581 tgcggatttc taacttcaat tactgttatg gcgctattgt tacttgtaat caaagcttct
    11641 tgtcccggtt ttaacaatat ttcattaata gagttagaca cgcttacact tccttcgatg
    11701 agatatgttt tacagaaatc attgttttcg aatgctttaa cactaaattg tgtccctaat
    11761 gctcgtattt cgcctagttt tgtttgaact gtaaatgggc gctgttgatt tttactaact
    11821 gaaaacaaag cttgcccgtt gagcttgata ttacgtttat atgagaaatt acgtttataa
    11881 gaaaccgatg tgttttttgc taattcaagt tgtgatccat ctggtaaaac aatattttta
    11941 atggttgccg tgttgtttga gatattaatt tcttgattga aataaatatt aaagcctaat
    12001 gccgaaattg aaacaataaa tgctacagcg gctgcaattt gtaaaaatgt aagtgtgaat
    12061 gttgtttgtt tttgctcgaa tgggatattt actttttcat tagcagcaat gcgctcttta
    12121 atttttgcga atgatgtctt cctatcttct tctgagacga caattgcgtc tttgaaccga
    12181 attttttcaa ttatatattt aattctttta tccatttttt gttattagct taataacact
    12241 aaacttaata tactttttaa aaactaaccc ttttccttgt agacgaaaga tttttgtttt
    12301 tgccttaaaa tgtaaaataa atcgtaaatt cgactaaatc atgccacctt ttaagggaaa
    12361 ataaaagatg aagaaaaatt atttttcaag tcaaaggtga aaaaagagta tttacccttt
    12421 gacctcatgc aataaatgct taattttatt ctcttaccct taaaaagtta caaagatgaa
    12481 acatttaacc gtagaacaaa gttacaaatt tgtctaaaat taaaaagttg catttctaac
    12541 ttgaatttag caatgatata ttttttttaa atgtaaagtg atttttttgt atgctatagc
    12601 tatttgagct tcaactgttt ttcgtgataa attgagcact tcagcaattt ctttatatgt
    12661 aagttgatcg tttattagca ttttgaaaat aagtttacat tgtggcggaa gttgctcgat
    12721 ggctatattt atatgctctt taaactcatt ttgttcaagt ggtgaaagga tatggtcgtc
    12781 tgcatctggt atttcaattt cgtataaact tgtatctgat tgaatgcgta ttttgttttt
    12841 acgaatggtg tcaattgata gatttttagc acaagtatac ataaatgatt taatagccct
    12901 gacatgattt aattctttgc gtttttgcca cagtgctaca aatacatctg atactacttc
    12961 ttcgcaaagt tgtttgtttc ttaaatagga ataggtgaag ttgcacaatg ccgaaaaata
    13021 cctagcatgt agctcttcaa aagcttcaat gtcatctgcc tcgtgtatgt ttttccacaa
    13081 tatttgatct tcagttgtca tttgttgatt ttatcccaaa ttgtccatta gaacaatatg
    13141 atattaggat tatttttttt agtcagt
//
LOCUS       DAOTAX010000056        12132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_4449_length_12132_cov_30.229776, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000056 DAOTAX010000000
VERSION     DAOTAX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_12132_cov_30.229776"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLM04_06605"
     CDS             complement(<1..827)
                     /locus_tag="PLM04_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HON02780.1"
                     /translation="MDRREFLKMLALSGAAFTIKQSKAMSLMTQHFSGKEINENYDLV
                     AVLGGTPVEMFRKGIAEFGGMEKFVKKGYKVVVKPNIGWDKTPEYAATTNPELVGEII
                     RQCFKVGAKEVTVFDHTCDDWRKCYASSGIEAAAKAAGAKVVPADLESYYESISLPRA
                     KNLKSAKVHKALLESDVWINVPILKNHGGAKMTISMKNLMGIVWDRGFFHSHDLQQCI
                     ADICTMSKPAVLNVVDAYRLMKTNGPRGKTEADVVLAKGMFMSQDIVAVDTAATNFF"
     gene            complement(853..1251)
                     /locus_tag="PLM04_06610"
     CDS             complement(853..1251)
                     /locus_tag="PLM04_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HON02781.1"
                     /translation="MEQKTFSEIMQRFADIRFDENFDLIVAIANGGIVPAGIINQKLN
                     KEIEILHINFRDDFQQPKYSTPQLLKPIDFEYLGKKILLVDDRIKTGATINVAKKLLE
                     GAQLIKTFAVNGNADYALYNESCFRFPWII"
     gene            complement(1257..1766)
                     /locus_tag="PLM04_06615"
     CDS             complement(1257..1766)
                     /locus_tag="PLM04_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HON02782.1"
                     /translation="MDTTIKLYHYNYRTLKTYLFATIFIVGNIIFPQLVHSIPNGGLI
                     FLPIYFFTLIGAYKYGFTVGLITAIISPLVNSWIFGMPPVGMLSIIITKSVLLAIGAA
                     YVASKSQKVSLVGLLLAILFYQLTGLMFEWFYTGSLILAYQDLRMGFPGLLLQWLGGY
                     ALLRSIQKL"
     gene            complement(2053..3324)
                     /locus_tag="PLM04_06620"
     CDS             complement(2053..3324)
                     /locus_tag="PLM04_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HON02783.1"
                     /translation="MQPLAERLRPQTLDQFIGQKHLVGENAILRQMIESGHIASLILW
                     GPPGVGKTTLARIIARQLNCPFYTLSAINSGVKEVREVIDKAKNSRFFSQQSPILFID
                     EIHRFNKSQQDALLGAVENGTITLIGATTENPSFEVITPLLSRCQVYLLTPLDKNDLL
                     ELAQRAIDTDEELKKKKIHLDETEALLRFSGGDARKLLNIIELVVTAEPSETVHITND
                     IVTRRLQQNPMAYDKNGEMHYDIISAFIKSIRGSDPDAAIYWLARMVAGGEDPKFIAR
                     RLVISAAEDIGLANPNALLLANACFDALQKIGWPEGRIILAETTVYLAASQKSNSAYL
                     AIDDALNLVEKTGDLPVPLHLRNAPTKLMKELGYGKDYKYSHDYENHFVVEQFLPDQL
                     KNVSIWKKQDNAAEKKMTEWLEKLWKNRYQK"
     gene            4034..5509
                     /gene="guaB"
                     /locus_tag="PLM04_06625"
     CDS             4034..5509
                     /gene="guaB"
                     /locus_tag="PLM04_06625"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HON02784.1"
                     /translation="MSFIAERVQFEGLTFDDVLLIPSYSDVLPREVDLSTRFTRHITL
                     KIPIVSAAMDTVTESNMAIAIAREGGIGVIHKNMSIAEQARQVEIVKRAENGMISNPV
                     TILKGATVGNALALMAEFKIGGIPVVDESGYLVGIVTNRDLRFQRDFYKPVEEVMTKE
                     NLITTSRSITLDEASEILQQYKIEKLPVVDDKNKLIGLLTYKDITKAKDKPMASKDKQ
                     GRLRVAAAVGVAADTFERMDALVAAEVDAIVIDTAHGHSKGVIDMLKKARKAYPNLDI
                     IVGNIATAEAARDLAEAGADAVKVGIGPGSICTTRVVAGVGVPQLSAIFEVSKALKDL
                     DVPIIADGGIRYSGDIVKALAAGGETIMAGSLFAGVEESPGETIIFNGRKFKSYRGMG
                     SLEAMEKGSKDRYFQDMEDDIKKLVPEGISGRVPFKGSLYEVVYQLIGGLRAGMGYCG
                     AHNINELHQARFTRITNAGVVESHPHDVTIISEAPNYSRGE"
     gene            5673..7619
                     /locus_tag="PLM04_06630"
     CDS             5673..7619
                     /locus_tag="PLM04_06630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON02785.1"
                     /translation="MKLSLLSLSAALSLSACLFAQTNDPVLMTINGHPVYKSEFEYIY
                     NKNNSNNSLDKKSLDEYVDLFINFKLKVEEAKAQGIDTTSAFINELAGYRSQLAKPYL
                     TDQKFEEKLMQEAYQRLKEDVEVSHILIRIPQNATPADTLAAWNKVKSILKRLEKEDF
                     AKVAKEVSEDETAEKTGGYIGWITAFQTFYPFETMAYNTPVGGISQPVRSIYGYHIIK
                     VHNRRNSVGEVQVAHIMRFTSPNDSVKNKRAKQVIDSLYQCVKNGEDFGALASKYSED
                     KPSAARNGELPWFGTGRMVPQFEAAAFSLKKVGDVSEPIQTPFGWHIIKLLGKKDLPS
                     FNDMKSDLERRIKQDERANLAQQSFVNQLKKEYNFRLVDENVQDFYKLLKGRTLNDSV
                     FLTEIGKLNKPLFTFADKQYTQHDFANFLQKNQFTQKSIPTEVIDEKLNQFINAELMA
                     YEDTQLEKKYDDFRFLMQEYHDGILLFEVSNREVWDKALRDTVGLASYFEQHKSDYKW
                     VKPHYKGRVIYCKDKNTFKTAKLIAKRLANDSIDKYLTSRLNSDSVQYVKIEKGLFVE
                     GDNKAVDKLVFNKKEKFTPDADYPYVFVVGKLLKETPEDYTDVRGAVIADYQDYLEKE
                     WIKNLRSKYSFSVDKNVLRTVKEN"
     gene            7700..8575
                     /locus_tag="PLM04_06635"
     CDS             7700..8575
                     /locus_tag="PLM04_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON02786.1"
                     /translation="MYSVFIRRFIYALILGSSVIFLACCKNRTSNTKFTDKPLVEVEG
                     NYLFRYQIEQIIPPNVNKKDSEELANNFIKNWATNVLMYERAKQNIEDISEIEKLVEE
                     YRKTLIIHAYQQKLIQQRLSTDISEEEIKSFYEKFGNQIVLNESYVKGLLLVVPISAP
                     QLNEVRKWVQQPTVKSLENIEKYRLKNAISYEYFMNKWVPFLEILKKIPLELTKTPAE
                     FLSANVFVETSDSTQIYLLKIQEYLNQGDKKPYELAKEEIKDILRHQQEVALIKQFEN
                     DLYNDALTKKKIIFY"
     gene            8644..10005
                     /locus_tag="PLM04_06640"
     CDS             8644..10005
                     /locus_tag="PLM04_06640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HON02787.1"
                     /translation="MQTKRFVFFILLVSFFPFIHLTAQNNVIDEVIWIVGDDAILKSE
                     VEEQRLRAQYEGVPIQGDPYCVIPEQIAIQKLFLHQAQLDSIVANESTVNSQVEARLN
                     YFVSQIGSKEKMEEYFGKKTTELREDLRQIIREQMIIQQMQQKLVGDINPTPAEVRRF
                     FKQLPEDSIPVVPAQVELQIISYQPPVSQQEINRVKQRLLDFTERINSGNADFSVLAR
                     LYSEDVESAKRGGELGFLGRGQLVPEFAAVAFNLQDPKKVSRIVETEFGFHIIQLIEK
                     RGDRINCRHILLRPRISEEEKEKALHTMDSIANLIRSGKITFEQAVMYYSQDKNTALN
                     AGLMLNEKTGTSKFEYQDLPPEISKIAYNMNVGEISNPFIMIDPKTHKEVIAIVKVKS
                     KIPNHKANLVDDYQLIKTFYENKKKEEFLRDWIIKKQKETYISIDPAWRNCEFQYPGW
                     IKN"
     gene            10075..11901
                     /locus_tag="PLM04_06645"
     CDS             10075..11901
                     /locus_tag="PLM04_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HON02788.1"
                     /translation="MNLLHLDSVSSFFQIKLLFPLFLMVGIITSPALAQNRPKQLREE
                     ILNSQKEAETKSAKSTKKKELSKPQTVLPPLTPVMPIVNPLENEKATLIYLENSETVT
                     FDKFLNPDIQVLKGNVQFRHENALMYCDSAYFYKATNSLDAFGNVRMVQGDTLFVYGN
                     VLYYDGFTKLARLRKNVRLENRKTVLTTDSMNYDRITNLAYYYTGGKIKDEVNTLTSV
                     WGQYSPATDQALFKDKVKLVNKNYTMDADTLKYNTKTNIANIVGPTHILYKNETDIYS
                     TRGWYNTSTERSMLLDRSLVIHKDGKTLTGDSIFYDKKQKYAEAFQRVILTDTVQKTT
                     LTGNFVSYNENTEFGMANDSALLVEWSTQDSLFLHADTLFTFKDSVYNAARAYYNVRF
                     FRNDLQGVCDSLYYSARDSVVRMYKEPVLWAEDFQITGDSISGYIKNKKIDHIRVLPS
                     AIAIQQFDSIHFNQISGKEIVAYLDSNQLKRVHVNGNAETIYYPLDEKDSTLTGLNKT
                     QSSYVVVYFKDKKVDRIVLTNASNGIMYPLNQLSGGDIYLKNYIWLDYLRPKSKKEVF
                     LKISRQPAARIGTSSLMNTTVNQSSSENTERNSRKSPGMRNP"
BASE COUNT         3916 a         2110 c         2367 g         3739 t
ORIGIN      
        1 ttgaaaaaat tggtggcagc cgtatcaaca gctacaatat cctgcgacat gaacattcct
       61 ttggcaagta caacgtctgc ctccgttttt cctcgcgggc cgttggtttt cattagtcta
      121 taggcatcaa ccacattcaa tactgccggt ttgctcatgg tacaaatatc ggctatgcat
      181 tgctgtaaat catggctgtg gaagaaacct ctgtcccaaa caattcccat gaggtttttc
      241 atggaaatgg tcatttttgc cccgccatga tttttcagaa tgggaacatt aatccacaca
      301 tccgattcca gaagagcttt gtgaactttg gccgatttca gatttttggc acgagggagt
      361 gaaatggatt cataatagga ttccaaatcg gctggaacta ccttcgctcc tgcagctttg
      421 gctgctgctt caattccgct gctggcatag cattttctcc agtcgtcgca agtatgatca
      481 aaaacggtaa cttctttggc accgaccttg aaacattgcc gaataatttc tcctaccaat
      541 tcaggattcg ttgtagcagc atattctgga gttttatccc agccaatgtt tggttttaca
      601 acaaccttat atcctttctt gacaaatttt tccattccgc caaattcagc aattcctttt
      661 cgaaacattt caaccggcgt tcctcccaac acggcaacaa gatcgtaatt ttcgtttatt
      721 tcttttccgc tgaaatgctg agtcatcagt gacatggctt tgctttgctt tatggtaaaa
      781 gcagctccac tgagtgcaag catttttaaa aattctcttc tgtccatagt gttgtttgtt
      841 taaaaacaaa tttcaaatta tccagggaaa acggaaacag ctttcgttat aaagagcgta
      901 atctgcattt ccgttaacgg caaaggtttt gataagttgt gctccttcca gcagcttttt
      961 tgcaacgtta attgttgcac cggttttaat ccgatcgtcc accaacaaaa tttttttccc
     1021 aagatattca aaatcaatgg gttttaatag ttggggagtt gaatattttg gttgttgaaa
     1081 gtcatcacga aaattgatgt gcaaaatttc gatttccttg ttcaacttct gattgataat
     1141 acctgcaggg acaattccac cgttggcaat agcaacaatc aggtcgaaat tttcatcaaa
     1201 tcggatatcg gcaaaacgtt gcatgatttc cgaaaaagtt ttctgttcca ttcaaattac
     1261 agcttttgaa tggatctcaa caaggcataa ccgcccaacc actgtagaag caatcccgga
     1321 aatcccattc tcaaatcctg ataggcaaga ataaggctgc cggtgtagaa ccattcaaac
     1381 atcagcccgg ttaactgata aaacagaatt gccaacaaca atccaacaag actcactttt
     1441 tgactttttg aagcaacata ggcggctcca attgccaata aaacagattt agtaataatg
     1501 atagacaaca tgccaaccgg tggcattcca aaaatccaac tgttgaccaa aggcgaaatt
     1561 attgccgtaa taagtccaac agtaaagccg tatttgtaag ctcctattag agtaaaaaag
     1621 taaatgggca ggaaaataag gccgccattg ggaatgctgt gtaccaactg gggaaaaatt
     1681 atattcccga caataaaaat agttgcaaat aaatatgttt ttaatgttcg ataattgtag
     1741 tgataaagtt taattgtggt atccatagca aatcatcatt attaaagaat taattttcaa
     1801 tcttagcata agacaaaaat ataaataaaa aacaaatcat tctccaaaat taagcaaatt
     1861 ttacagtcat tttaaattga aaatgaaaat tataatgtta ttcgctcgct cctgccgatt
     1921 tgtaatcggt ggtttggtct tgttcggcgt agcctcctgg ctacgtcgtc agtaaaaata
     1981 aaatttccag attttttaat aagatatgat tttgatagga tttgcctttt tacttgagag
     2041 aaagaaattt ttttattttt ggtatctgtt tttccaaagc ttttccaacc attcagtcat
     2101 ttttttctct gcagcattat cctgtttttt ccaaatgctg acatttttaa gctggtcggg
     2161 aagaaactgc tctactacaa aatgattttc gtaatcgtgt gaatatttgt aatcttttcc
     2221 gtaaccaagt tctttcatga gtttggtagg tgcattcctt aaatggagtg gaactggcag
     2281 atctccggtt ttttctacca gattcaaagc atcgtcaata gccagatagg ccgaattgct
     2341 tttttgagaa gctgccagat aaactgtagt ttcggcgaga ataatccttc cttcgggcca
     2401 tcctattttt tgcagtgcat caaaacaggc atttgccagc aataaagcat tgggattggc
     2461 aagaccaata tcttctgcag cagaaatcac caatctacga gcaataaatt tcggatcttc
     2521 accgcccgct accattcggg caagccaata aatggcggca tcagggtcgc tgccacgaat
     2581 ggatttgata aacgcagaaa taatatcgta atgcatttct ccatttttat cgtaagccat
     2641 tgggttttgc tgcagtcggc gggtaacaat atcattggta atgtgaaccg tttcggaagg
     2701 ttcggcagta acaaccagtt ctatgatatt cagtaatttg cgggcatcac ctcccgaaaa
     2761 acgcagcaaa gcttccgttt catccaaatg aatttttttc tttttcagtt cttcatcggt
     2821 atcaatggct ctttgagcca attccaacaa atcgttttta tccaatggag tgagtagata
     2881 aacctgacag cgggagagga gaggcgtaat tacttcaaag gaaggatttt ctgtggtagc
     2941 accaattaaa gttatagtgc cattttctac ggctccaagt aaagcatcct gttgcgattt
     3001 gttgaaacga tgaatttcgt cgataaacaa aataggactt tgttgcgaaa aaaaacgact
     3061 gttcttggcc ttgtcgatta cttctctgac ttctttcact cctgaattga tggcacttag
     3121 cgtataaaag ggacaattca gttgtcgggc aatgattctt gccaacgttg tctttcccac
     3181 gccgggcggg ccccataaaa tcaaggaagc aatatgcccc gattctatca tctgacgcaa
     3241 aatggcattt tcgccgacca aatgtttttg tccgataaat tggtcaagtg tttgtggacg
     3301 aagtcgttct gctaaaggct gcatgaaaat ttgttttgaa ttactattac aaatttagtt
     3361 cttaattttg atttttttga tttttcagtt gaaaaattca tgaagtttat agtttttgag
     3421 ttagcaattt atgagattca aggttgcgag tttcgagtag aaatattttt gaaaaaagtt
     3481 cggcgcagcc tcaaagctac gtcgaattaa aaataaaatc tccagatttt caataagcga
     3541 ataaggtttt ggatggtagg aggatttgct tgttatgaag gtttttttga aaaataaggt
     3601 ttaaagaatt gaccttgcct ggtgagccga accaacggtc agcgtagcta aaaatcagtg
     3661 aagttggcgt taaaaatctt ttctgtttga gcgacggagg agcgagttta gaagatttta
     3721 gccaacgaac gtcaattttt cttgtaagtg gggtttgggg caatgccgcg ttattaaata
     3781 tctgaaaata aataatgtaa atatttgtac aattttttgt catgtactaa gtaattcatt
     3841 accgaaaatt ttttcaatca aatctttaaa tttcggctta tatagaaccc gaagatgggg
     3901 tatcatatta gtttttcaaa tgattttttc catagtaaca aataaatatg taaatttgca
     3961 tgcaataatt tcataggtta tatgaatata tattatttat ctcattaaaa atcaattaaa
     4021 taaatttatt tctatgtcat ttattgcaga aagagttcaa tttgaaggtt taacatttga
     4081 cgatgttttg cttattccat catattccga tgttttacct cgtgaagttg acttatccac
     4141 acgttttact cgccatatca ctttgaaaat ccccatagtt tcggcagcta tggatacggt
     4201 aacagaatca aatatggcta tcgccatagc cagagaaggt ggaataggag tgatccacaa
     4261 aaatatgtcc attgctgagc aggcacgaca ggttgaaatt gttaaacgcg cagaaaatgg
     4321 aatgatttcc aatccggtaa ctattttaaa aggtgctact gttggcaatg cgctggcttt
     4381 gatggccgaa tttaaaattg gtggtattcc tgttgtagat gaatcaggtt atttggtggg
     4441 aatagtgacc aatcgcgatt tgcgttttca gcgcgatttt tataaacctg tggaagaagt
     4501 tatgacgaag gaaaatctga ttacgacttc ccgttccatt actttggatg aagcttccga
     4561 aattttgcaa caatacaaaa tcgaaaaatt acctgttgtc gatgacaaaa acaagctgat
     4621 tggtctgttg acctataaag atattacgaa agccaaagat aaaccgatgg cctcgaaaga
     4681 caaacaggga cgtttaagag ttgctgctgc agtgggagtt gcagccgata cttttgagcg
     4741 tatggatgca ttggttgctg cagaagtgga tgctattgtt attgatactg ctcatgggca
     4801 ctcaaaaggg gttatcgata tgttgaaaaa agcacgcaaa gcatatccca atcttgatat
     4861 tatagttgga aatatagcaa cagctgaagc agctcgcgat ttggccgaag ccggagcaga
     4921 tgccgttaaa gttggtattg gtccaggttc tatttgtaca acacgcgtag tagctggagt
     4981 tggcgttcca cagttgtctg caatttttga agtttctaaa gcgctcaaag atttggatgt
     5041 ccccattatt gcagatggcg gcattcgtta ttccggcgac attgttaaag ccttagcagc
     5101 gggaggagag acgatcatgg caggttcact ttttgccgga gttgaagaat caccgggaga
     5161 aaccattatt tttaatggac ggaaatttaa gtcctatcgt ggaatgggtt cgctcgaagc
     5221 gatggaaaaa ggttccaaag accgctattt ccaggatatg gaagacgata ttaaaaagct
     5281 tgttccggaa ggaatctccg gtcgtgtacc atttaaaggt tctttatacg aagttgttta
     5341 tcagttaatt ggcgggttgc gcgcaggcat gggttattgc ggagcgcata acattaacga
     5401 attgcatcag gccagattta ccagaattac caatgcgggt gtagtagaaa gtcatcctca
     5461 tgatgttacc attatcagcg aagcgccgaa ttacagcaga ggcgaataaa agcttaaaat
     5521 tcaaaaaaaa tcaaattttt acaacattat cttttttgtt tcgttaattt ttttgcagtg
     5581 cgatgaagat tttgctttct aaagctgata aagcaagttt ttaagcacaa tgtttcaaat
     5641 tagtgagtaa aaatattaat cgttttttat ctatgaaatt atcccttctt tctctatcgg
     5701 cagcattgtc gctttcggca tgtttgtttg cacagaccaa tgatccggta ttgatgacca
     5761 ttaatgggca tccggtctat aaatcggaat ttgaatacat ttacaacaaa aacaattcca
     5821 acaattctct tgataaaaaa tcattggatg aatatgtgga tttgtttatt aatttcaaat
     5881 tgaaagttga ggaagcaaaa gctcagggaa ttgatacaac ttctgctttt ataaatgagt
     5941 tggcaggtta tcgttcacaa ttggcaaaac cttatctgac cgatcaaaag tttgaggaga
     6001 aattgatgca ggaagcttat cagcgactga aagaggatgt ggaagtgagt catattctca
     6061 ttcggattcc gcaaaatgca actccagccg atactttggc agcttggaac aaagtaaagt
     6121 ctattttaaa acgtttggaa aaagaagatt ttgccaaagt agccaaagaa gtatctgaag
     6181 atgaaacagc ggagaaaacc ggtggttata ttggctggat tacagcattc cagacttttt
     6241 atccttttga aacaatggct tacaacactc ctgtgggtgg aatttcacaa cctgttcgtt
     6301 ccatttatgg ctatcatatt ataaaggttc acaacagaag aaattcggtc ggtgaagttc
     6361 aggttgcaca catcatgcga tttacttctc ccaatgacag tgtaaaaaac aaacgtgcca
     6421 aacaggttat cgattcgttg tatcagtgcg tgaaaaatgg cgaagatttt ggagctttgg
     6481 caagcaaata ttctgaagat aaaccttctg cagcccgaaa cggtgaatta ccctggtttg
     6541 gaacggggcg aatggttcca caatttgaag ctgctgcttt ttcattgaaa aaagtgggcg
     6601 atgtttccga accaatacaa acgcctttcg gctggcatat tattaaacta ctcggaaaaa
     6661 aggatttacc ttctttcaat gatatgaaat ctgatttgga aagacgaatc aaacaggatg
     6721 aacgggcaaa tcttgctcag cagtcgtttg tcaatcaact aaaaaaagaa tataatttcc
     6781 gtttagtgga tgaaaatgta caagattttt ataaattatt gaaagggaga acattaaatg
     6841 attccgtatt tttgactgaa atcgggaaat taaataaacc attatttacc tttgccgaca
     6901 aacagtacac ccagcacgat tttgcaaatt tccttcagaa gaatcagttt actcaaaaaa
     6961 gcattccgac agaagtaatt gacgaaaagc tgaatcagtt tattaatgca gaattaatgg
     7021 cttatgaaga tactcaattg gagaagaaat atgacgattt cagatttctc atgcaagaat
     7081 atcacgacgg cattctgttg tttgaagtta gcaatcgaga agtctgggat aaagcgctga
     7141 gagataccgt tggtttggct tcctattttg agcagcacaa gtcagattat aagtgggtaa
     7201 aacctcacta taaagggcgt gtgatttatt gcaaggacaa aaacactttc aaaaccgcta
     7261 aactgattgc caaaagattg gccaacgatt ccatagacaa atatttgacc agccggctga
     7321 atagcgactc tgttcagtat gtaaaaattg agaaggggct ttttgtggag ggagacaaca
     7381 aggcagtcga caagctggta ttcaataaaa aggaaaagtt tactcctgat gccgattatc
     7441 catacgtctt tgtagtcggt aaattgttaa aggaaacgcc tgaagattat actgatgtca
     7501 gaggagctgt aattgccgat tatcaggact accttgaaaa agaatggata aaaaatcttc
     7561 gttcaaaata tagtttttcg gttgataaaa atgttcttag aaccgttaag gaaaactaat
     7621 aatttttgac aggcgacggg aaattactaa ttttatcttt ccaatttcga gaaatcgaaa
     7681 cgaattatat aattgtttga tgtacagtgt ttttattcgg aggtttattt atgctttgat
     7741 attgggaagc agtgtgattt ttttggcttg ttgcaaaaat cgtacttcga acacgaaatt
     7801 taccgataaa ccacttgtag aagtggaagg aaactatctt tttcgttatc aaattgaaca
     7861 aatcattcct cccaatgtaa ataaaaaaga tagtgaagag ctggcaaata attttatcaa
     7921 aaattgggct acaaatgtct tgatgtacga acgtgccaag caaaatattg aagatatttc
     7981 cgaaattgaa aaattggtag aggaatatcg taaaacatta attattcatg cataccagca
     8041 aaagttaatt caacaaaggc tctcaacaga catttctgag gaagaaataa aatcatttta
     8101 cgaaaaattt ggtaaccaga tagtgctcaa tgaatcttat gtaaaaggat tattacttgt
     8161 ggtgcccata tctgctcctc aattaaacga agtgagaaaa tgggtgcaac agcctacagt
     8221 aaaatcattg gagaatatag aaaaatatcg attaaagaat gctatcagtt acgaatattt
     8281 tatgaataag tgggtccctt ttttggaaat tttgaagaaa atccctttag aattgacaaa
     8341 aacgcctgca gaatttttgt cagcaaatgt ttttgttgaa acttcagatt ctactcaaat
     8401 ttatttatta aaaattcagg aatatcttaa tcaaggtgat aaaaaaccgt atgaattggc
     8461 aaaagaagaa attaaagata ttttgagaca tcagcaagaa gtagctctca tcaaacagtt
     8521 tgaaaacgat ttgtataacg atgctttaac caagaagaaa ataatttttt attaaacagt
     8581 aattagtatt ctcaattatt ctgatcttgt tttcttttta tttttttaat tttaattagt
     8641 ttcatgcaaa caaaaagatt tgtttttttt attctacttg tttccttttt cccatttatc
     8701 catttgacag ctcagaacaa tgtgattgat gaagtcattt ggattgtagg agatgatgct
     8761 attttgaaat cggaagtaga ggaacagcgt ttgcgtgctc aatatgaagg cgtccccatt
     8821 caaggagatc catattgtgt gatacccgag caaatagcca ttcaaaagtt atttttgcat
     8881 caagcccaac ttgatagtat tgtagccaat gaaagcacag taaattccca agtggaagct
     8941 cgtttaaatt attttgtttc acaaattggc tctaaggaaa aaatggaaga atattttggc
     9001 aaaaaaacca ccgaattgcg agaagatctt cgtcaaataa ttcgggaaca gatgatcata
     9061 cagcaaatgc agcaaaaact ggttggagac ataaatccta ctcctgctga agtgagaaga
     9121 tttttcaaac aacttccaga ggatagcatt cctgttgtac cggctcaagt tgaattacaa
     9181 attatcagtt atcagccacc tgtatctcaa caggaaataa accgagtaaa acagcgttta
     9241 ctggatttta ccgagcgtat taatagtgga aatgctgatt tttctgtatt ggccaggctt
     9301 tattccgaag atgtggaaag cgccaagcgt ggaggagaat tgggtttttt gggtagagga
     9361 caactggtac ccgaatttgc agctgtagct tttaatttac aggatcccaa aaaagtatcc
     9421 cgcattgtag aaacagaatt tggtttccat atcattcaac tgatagaaaa acgaggtgat
     9481 cgtataaatt gccgacatat attgctcagg ccacgcattt cagaagaaga aaaagaaaaa
     9541 gccttgcaca ctatggattc cattgccaat ttaataagaa gcggaaaaat tacttttgaa
     9601 caggcagtaa tgtattattc gcaagataaa aacacggctt tgaatgcagg cctgatgctg
     9661 aatgaaaaaa ccggaacttc aaagtttgaa taccaagatc tcccgcctga aatttctaaa
     9721 atagcttata atatgaatgt tggagaaatt tcaaatcctt ttataatgat tgatcccaaa
     9781 acccataagg aagtgatagc tatagtaaaa gttaaatcaa aaattcctaa tcataaggca
     9841 aatttagtgg atgattatca actgattaaa actttttatg aaaacaaaaa gaaagaagaa
     9901 tttcttcggg attggattat taagaaacag aaagagactt atatcagcat tgaccctgca
     9961 tggagaaatt gtgagttcca ataccctggt tggataaaaa attaaatcat gtgttgatgc
    10021 ttatattgct ttcaagttat tcgcatttgt tttttaatag tataatcatt tttcatgaat
    10081 ttactccatc ttgatagcgt ctcttctttt tttcaaataa aattgttatt tccacttttt
    10141 ttgatggtgg gaataataac ttcccccgct ttagctcaaa ataggccaaa gcagttgcgc
    10201 gaagaaattc tgaatagtca aaaagaagca gagacgaaat ctgcaaaaag tacgaaaaag
    10261 aaggaacttt caaaacctca aaccgttctt ccgcctctaa ctcctgtaat gcctattgta
    10321 aatcctctgg aaaacgaaaa agctacctta atttaccttg aaaattctga aaccgtaact
    10381 ttcgataagt ttttaaaccc cgatattcag gttttgaagg ggaatgttca attcaggcac
    10441 gaaaatgctt taatgtattg cgatagtgcg tatttttaca aggctaccaa ttcattggat
    10501 gctttcggta atgtaagaat ggtacaggga gacaccttgt ttgtgtatgg aaatgttctt
    10561 tattatgatg gtttcaccaa gttggcgcgt ttacgaaaaa atgtgaggct cgaaaatcgg
    10621 aaaacggttc ttactaccga cagtatgaat tacgaccgaa ttacaaatct ggcttattat
    10681 tataccggag ggaaaataaa agatgaagta aataccttga cttctgtttg ggggcaatac
    10741 tcgcctgcaa ctgaccaggc tctgtttaaa gataaagtaa agctggtaaa caaaaattac
    10801 acaatggatg ccgatacgtt gaaatacaac acaaaaacaa acattgccaa tattgtcggg
    10861 ccaacgcata ttctttataa aaacgaaacc gatatttatt ccactcgtgg atggtataat
    10921 accagtaccg aacgttccat gttgttggac agatcgttag taattcataa agatggaaaa
    10981 accctcacgg gcgattcaat attttacgat aaaaaacaaa aatatgccga ggcttttcaa
    11041 agagtaatat tgactgatac agtgcagaaa accaccttaa cgggaaattt cgtttcttat
    11101 aatgagaata ccgaatttgg tatggctaac gattcggcat tgcttgtcga gtggtcaact
    11161 caggatagtt tgtttttgca tgccgacacg ctgttcactt tcaaggattc cgtatacaat
    11221 gcagctcgcg catattataa cgtacgtttt ttcagaaacg atttgcaggg agtttgcgat
    11281 tccctttatt actcggctcg agattccgta gtccgtatgt acaaggaacc cgttttgtgg
    11341 gcggaagatt ttcaaataac aggggattcg ataagcggtt atattaaaaa taaaaaaatt
    11401 gatcatattc gtgttttgcc ttctgcaatt gccatacagc agttcgactc cattcatttt
    11461 aaccagattt cgggaaaaga aattgttgct tatttggaca gcaatcagtt gaaacgtgta
    11521 catgtaaatg gaaatgccga aacaatatat tatccgcttg acgagaaaga ttcaactctc
    11581 actggactca ataaaacaca aagcagttat gtagtggtat attttaagga taaaaaagtg
    11641 gatagaattg tcttaaccaa tgcatcaaat ggtatcatgt atccactcaa tcaactttcg
    11701 ggaggcgata tctatctgaa aaattatatt tggcttgatt atttgagacc taaaagcaaa
    11761 aaagaagttt ttttgaaaat ttccagacaa cctgctgccc gaataggtac cagcagcttg
    11821 atgaatacta cagttaatca gtcatcatcc gaaaatacgg aaagaaattc gagaaaatct
    11881 ccgggtatgc gaaatccata attttgattt aaatgtcatg tgcatactct ttctgaatcg
    11941 ttttatttct attttagaaa tagttttttg tttttttgtc catcaaaata ataagaaact
    12001 atcttcaatg aacacatcct tctcctgttg catccgggtt caacttcaaa aacagaaaaa
    12061 atctttggaa aaaatttaaa tgattatccg caatgattcc tattgtaaat tcttgacttg
    12121 aaatcggagt tg
//
LOCUS       DAOTAX010000057        12039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_4489_length_12039_cov_32.468208, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000057 DAOTAX010000000
VERSION     DAOTAX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_12039_cov_32.468208"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLM04_06650"
     CDS             <1..360
                     /locus_tag="PLM04_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON02789.1"
                     /translation="SESQKLRAEILFREYPDLKQGYSLTHSLRMIYNKNSIKAAARLS
                     LAKWYNNVAESQFKSFNTIAATVYEHHEEVLNFFINRSTNASAESFNAKIKSFRASLR
                     GVIDIKFFLFRLTNIYA"
     gene            complement(479..562)
                     /locus_tag="PLM04_06655"
     tRNA            complement(479..562)
                     /locus_tag="PLM04_06655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:Leu,seq:caa)
     gene            complement(688..1896)
                     /locus_tag="PLM04_06660"
     CDS             complement(688..1896)
                     /locus_tag="PLM04_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HON02790.1"
                     /translation="MKITSGNDNKIRKFFAEKEFPMIAKTFQGLEEVLAEELTELGAN
                     NVQIERRAVSFSGDKAMLYKANLHCRTALRILKPIFSFEADNTDELYKKIKLVEWDVL
                     LHVNNTFSVDATVFSDEFQHSRFVTYRVKDAIVDWFSENFNDRPSVSVEDPDFVFNIH
                     IAGSHCTLSLDSSGESLHKRGYRTGQTKAPLNEVLAAGMLMLAGWKGQSDFLDPMCGS
                     GTLLIEAALIALNIPPGIFRASFAFEKWPDFDKVLFDDLYNDDSQELLFEHHIFGSDI
                     SVSAIKIAQQNVKSSGLSKYISLKVLPLEKLEPPSSDCFIVTNPPYGERLPSKDIFEL
                     YANLGSLLKRKFSGCTAWVISSNEEALKKIGLKPSKRYRLMNGPLECWFQQYELFSGK
                     RNDYLQKKNS"
     gene            complement(1907..2797)
                     /locus_tag="PLM04_06665"
     CDS             complement(1907..2797)
                     /locus_tag="PLM04_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HON02791.1"
                     /translation="MEKEILDAIIELSKIDQYKFACHQHVRNERMPSINNLQEIVQLC
                     RSVLFPGYFDDAALDSDTLLYHIGLSIDRLKLLLTEQLKAGFCFEREHSTDKNLCSDM
                     EQNVVQKVNAFIAQLPEIRRKLYTDVIAAYNGDPAAKSIGEVIFCYPGIRAISNYRIA
                     HALLILDVPLIPRIISELAHSETGIDIHPGAEIGDYFMIDHGTGVVIGETSVIGNNVK
                     MYQGVTLGARSFPLDENGNPIKGIVRHPRIGNNVIIYSNSTILGNITVGDGAVIGGNL
                     WVDSDVPAGAKLSQDDKLKR"
     gene            complement(2816..3343)
                     /locus_tag="PLM04_06670"
     CDS             complement(2816..3343)
                     /locus_tag="PLM04_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HON02792.1"
                     /translation="MLRVAILGPESTGKTELAKSLAEYFQVDWVPEFARAYVESLSRP
                     YEYEDVCRIAAHQIDEELFYENHPSDGKKFVFFDTDLIITKVWFDYKYGKVPKFLQER
                     LNCHFFDFYLLCYPDLPWQPDSVREHGDDREFFFNWYKQEIEKTGKSVSIITGIGNER
                     LQNAIRALTSFVSYL"
     gene            complement(3369..3992)
                     /gene="pnuC"
                     /locus_tag="PLM04_06675"
     CDS             complement(3369..3992)
                     /gene="pnuC"
                     /locus_tag="PLM04_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HON02793.1"
                     /translation="MILLKETFSLNPVIEYVIDNWVELTGAILSLIYLILSVRQKIGL
                     WIFGFLCAALYVYVFFQSKFYAGMTLQFYYLAVSIYGWITWKRGKTDTQPELPVTRTS
                     KHMAFNLLTVFLAILVIYFFILSNFTDSPLPRGDAFTTALSIVATWMLAKKLLENWLL
                     WIIADAVSTGLYLYRGLYPTAVLFTVYTLMAVLGYFQWKKSMKHQTH"
     gene            complement(4006..5589)
                     /locus_tag="PLM04_06680"
     CDS             complement(4006..5589)
                     /locus_tag="PLM04_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON02794.1"
                     /translation="MKKLYLVFIFNLILAGVFGQGISLMQQRKLMNALNAISSLYVDS
                     INDKKLIESAITALLKDLDPHSSYIPREEVERINEPLEGEFEGIGIQFQIFEDTLMVV
                     QTIAGCPAEKVGILPGDRIIYINDELVAGVKIQNSDILKRLRGPRGSEVTVKVLRRGK
                     PELLIFKIIRDKIPLYSVDAQYMIGNDIGYLKINSFGRTTDEEFVKAFTELQKKGMKH
                     LIVSLQGNGGGYLATAIQLADQFLDRGKLIVYTEGFHQPKKESLATAVGKYETGKLVI
                     LVDEYSASASEIVSGALQDWDRAVIVGRRTFGKGLVQQQLPLVDGSMMRLTTARYYTP
                     SGRCIQKPYKDGADKYERELLTRYKKGELIHPDSIHFPDSLRYQTLRLKRTVYGGGGI
                     MPDVFVPLDTTRFTDYHQKLVARGVINKVSVQFIDQNREDLKRKFSTFDKFEKEFTVD
                     EAFLQQLIQAGEKENIPFQSSQFDTSRELIKLQLKALIARDLWDMNAYYRIINNDNEV
                     LKKAVELLQTPNAYEQILK"
     gene            complement(5709..6347)
                     /locus_tag="PLM04_06685"
     CDS             complement(5709..6347)
                     /locus_tag="PLM04_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HON02795.1"
                     /translation="MNIFVDIHTHGKSEFNPYSVRSLQIKEAALFFDTEERRFCSVGI
                     HPWNAEQENLEDLERLFRYSSDTRWIAIGECGLDKNISTPFATQKSIFEGQISYSESL
                     KKPLIIHCVGYFNELLEIRNKFRPEQLWIVHGFRGKPELAKQLLKTGISLSFGEKRNA
                     ASVAVTPIDRLYVETDESLLPVEQFYREIAEIKNCRPENLNAGKKLLHLKNI"
     gene            6481..7905
                     /gene="ahcY"
                     /locus_tag="PLM04_06690"
     CDS             6481..7905
                     /gene="ahcY"
                     /locus_tag="PLM04_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HON02796.1"
                     /translation="MTDNKALFTSLPYKVADISLADFGRKEIELAEKEMPGLMAIREK
                     YAAQQPLKGARITGSLHMTIQTAVLIETLAKLGAEVRWCSCNIYSTQDHAAAAIAAAG
                     ISVFAWKGETLEDYWWCTLQALTFEGGKGPNLIVDDGGDATLMIHTGVAAEKDANVLN
                     RPAEGEDEKELLAILKKVLKENSNFWSNITPEIRGVSEETTTGVHRLYQMLAEGTLLF
                     PAFNVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVCGYGDVGKGCAKSMR
                     AYGARVLVTEIDPICALQAAMEGFEVCTVEDALPEGNIYVTATGNRDIITIQHLEKMK
                     DGAIVCNIGHFDNEIQVDQLKKFPGIQKVNIKPQVDKYIFPDGHSVLLLADGRLVNLG
                     CATGHPSFVMSNSFTNQTLAQIELFTKKYEINVYRLPKHLDEEVARLHLGQLGVKLTQ
                     LTKEQAEYIGVNPEGPFKPEHYRY"
     gene            8159..9052
                     /gene="folD"
                     /locus_tag="PLM04_06695"
     CDS             8159..9052
                     /gene="folD"
                     /locus_tag="PLM04_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HON02797.1"
                     /translation="MYTLLDGKLISEQIKKEIAEEVQQMIKAGKKRPHLAVIIVGHDG
                     GSESYVAGKIKDCQICGFQSTLIRLEEDVTEQRLLQEVERLNEDTDVDGFIVQLPLPK
                     HISEQKMIEAINYKKDVDGFHPINVGRLCIGLPCYVSATPMGIMELLKRYRIETSGKN
                     CVVVGRSNIVGKPTAMLLMQKTYPGDATVTICHSHTKNLKEICQQADIIVAAIGQPEF
                     LKGDMVKEGAVVIDVGTTRVPSTETKSGFKLKGDVDFNEVAPKCSFITPVPGGVGLMT
                     RVSLMKNTLLAAKAEIYEKNG"
     gene            9136..9864
                     /locus_tag="PLM04_06700"
     CDS             9136..9864
                     /locus_tag="PLM04_06700"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HON02798.1"
                     /translation="MTVFYAPEVAFNNVLPKEESQHAIKVLRLKKGDKIVIVDGKGNG
                     YLAEILFPDSNHCEFEILETLPDEGNVHYHLHIAIAPTKNVERFEWFVEKATEIGIHE
                     ITPIICKFSERKMIKPERLEKIVISACKQSMRAQFPVVHSLCSFQEVLTTYSAQQKFI
                     AHCYTDQIMKEFDCVRKRNFLKNLIEPSTKIFILIGPEGDFSIDEVKTAIEKGFDPVS
                     LGVGRLRTETAGIVACTTVALINQ"
     gene            complement(10219..10452)
                     /locus_tag="PLM04_06705"
     CDS             complement(10219..10452)
                     /locus_tag="PLM04_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HON02799.1"
                     /translation="MHLIFSILILIVFIGVLFLLFVLGFIRSFFNFGRKSKKRKQSKE
                     DNNIFTHSSKKKKIFDKNDGEYVDFEEIEEETK"
     gene            complement(10471..11421)
                     /gene="metF"
                     /locus_tag="PLM04_06710"
     CDS             complement(10471..11421)
                     /gene="metF"
                     /locus_tag="PLM04_06710"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HON02800.1"
                     /translation="MSIVDKIKSSSSTAFSFELLPPLKGDGIASVFKTIDLLREFDPK
                     YINITTHRSEVIFKQNDQGLFEQSDVRRRPGTVAIAAAIKSKYKIPVVPHVICSGFTK
                     EEIEYELIDLQFLGITDLLLLRGDKAKHEKDFVPTGHMHATDLQQQVNNFNKGIFLDG
                     SRMKALPAEPFSYGMAAYPERHEESPNTESDLYYTKMKVDGGAEYLVTQMFFDNQKYY
                     DFVEKCRNMGITVPIIPGIKPITLMNQLTVLPKIFHTEIPEEFASELRKCKTDAEATE
                     VGVEWCTKQASDLKAHGVPSIHFYSLMAAQSVKRVAKAVF"
     gene            11657..11794
                     /locus_tag="PLM04_06715"
     CDS             11657..11794
                     /locus_tag="PLM04_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02801.1"
                     /translation="MEKACGLDVHKDSIFACILDEQGKKILEKRYGTLTADLMTPFVI
                     H"
BASE COUNT         3800 a         2429 c         2281 g         3529 t
ORIGIN      
        1 tcggaatccc aaaagttgcg tgcagaaatt cttttcagag aatatccaga cctgaaacag
       61 ggttattctc ttacacactc cttgagaatg atttacaaca aaaacagtat caaagccgct
      121 gcaagacttt cattggctaa atggtacaat aatgtggctg aaagccaatt caagtctttt
      181 aatacaatag ctgctacagt ttatgaacat cacgaagaag ttcttaattt ctttatcaat
      241 agaagtacca atgcctctgc cgaaagtttc aatgctaaaa ttaaatcctt cagagcttct
      301 ttgagaggag ttatcgatat taaattcttt ctcttcagat tgactaatat ctatgcttag
      361 acacatgttt ttgcaggtga gccataaaat tgctccgcaa aaatcatcat tttgttgaaa
      421 tgttacaacc ataaaaataa aatcagcaga aaatcaaatg attaactgct gattaaagta
      481 cccagagccg gggtcgaacc ggcatggaag tgaatccact ggtgtttgag accagcgcgt
      541 ctaccgattc cgccatctgg gcattagcgg gtacaaaagt aaattatttt ttttattccc
      601 aaaagcaaaa atgccgaaat ttattaattt caatcaaaaa agaaacttta ataactaata
      661 ttcaaaagat tataaataca actgtaatta ggaattcttt ttctgcaaat aatcgtttct
      721 tttacccgaa aaaagttcat actgctgaaa ccagcattcc aaaggtccgt tcattaagcg
      781 ataacgtttt gaaggtttta aaccaatttt tttcagtgct tcctcattgg aactgatcac
      841 ccaagcagta caacccgaaa atttacgttt cagcaatgat cccaagttgg catataattc
      901 aaaaatgtct tttgatggga gcctttcgcc ataaggtgga ttggtaacaa taaaacaatc
      961 ggatgaaggc ggttcaagct tttccaacgg cagtaccttt aaagaaatat attttgacag
     1021 tccgctactt ttaacattct gctgggcaat tttaatggca gatactgaga tgtccgatcc
     1081 gaaaatatga tgttcaaaaa gtagctcctg tgaatcatca ttatataaat catcaaaaag
     1141 caccttatcg aaatcgggcc atttttcaaa agcaaaagaa gctctgaaaa taccgggagg
     1201 gatattcaag gcaatcaagg ctgcctcgat aagcaaagtt ccggagccgc acatcggatc
     1261 aagaaaatcg gattgtcctt tccagccggc caacatcaac attccggccg ccaaaacttc
     1321 attcagcgga gcttttgtct gtccggttct ataaccgcgt ttatgaagcg attcaccgga
     1381 actgtcgagc gacaaagtac aatggcttcc ggcgatgtga atattaaaca cgaaatcggg
     1441 gtcttcaacc gaaacacttg gccgatcgtt aaaattctct gaaaaccagt caactatggc
     1501 atctttcacc cgataggtta caaaacgcga atgctgaaat tcatcagaaa aaacggttgc
     1561 atcaaccgaa aaagtattat ttacatgcag aagaacgtcc cattccacca actttatttt
     1621 tttgtataat tcgtctgtat tgtcggcttc aaaagaaaaa atcggtttaa gaatacgtaa
     1681 ggcggttctg caatgcaaat ttgctttgta caacattgcc ttatcaccgc taaagctcac
     1741 tgcgcgccgc tctatttgca cattgttggc tcccaattcg gtaagttctt cagccaatac
     1801 ctcttcgagt ccctgaaagg tcttggcaat cataggaaac tccttctcgg caaagaattt
     1861 cctgatttta ttgtcgtttc cggaagttat tttcatcggt taacaatcaa cgtttcaatt
     1921 tatcgtcctg cgaaagcttt gctccggccg gaacatcgct atccacccaa agattaccgc
     1981 caattacggc accatcacca acggtgatat ttcccaaaat ggtagaattg gaataaatga
     2041 ttacattgtt gccaattcgt ggatggcgaa cgataccttt tatgggattt ccattctcgt
     2101 cgagcggaaa acttctggct cccagcgtaa cgccttgata cattttcacg ttgtttccaa
     2161 tcaccgaagt ttctcctata accacacctg tgccatgatc tatcataaaa taatctccaa
     2221 tttcggctcc gggatgaata tcgataccgg tttccgaatg agccagctca ctgataatac
     2281 gtggaatcaa aggaacatcg agtatgagta gcgcatgagc aatacgataa ttgctgatgg
     2341 ctcgaattcc cggataacaa aaaatcactt ctccaatact tttggcagca gggtcgccat
     2401 tgtaagcggc aatgacatcg gtataaagtt ttctgcgaat ttcaggcaat tgagctatga
     2461 aagcattcac tttttgaaca acgttctgct ccatgtcgct gcataaattt ttatctgtgg
     2521 aatgttctct ttcgaaacag aatcccgctt tcaattgctc ggtaagcaaa agtttcaatc
     2581 gatcgatact aagcccgata tgatagagca acgtatcgga atcgagcgct gcatcgtcaa
     2641 aatagcccgg aaacaaaaca gaacggcaca actgaacaat ttcctgcaaa ttattgatgg
     2701 aaggcattcg ctcatttcta acatgctgat gacatgcaaa tttatactga tctattttcg
     2761 ataattcgat gatagcatct aaaatttcct tttccataaa tttgaataat aaaatttata
     2821 aataactcac aaaagaagtc aaagcccgaa tggcattttg gagcctttca ttgccgattc
     2881 cggtgataat agaaacagat tttcctgttt tttctatttc ttgcttatac cagttgaaaa
     2941 agaattctct gtcgtctcca tgttcacgaa ccgaatcggg ttgccacggc aaatcgggat
     3001 aacacaacaa ataaaaatca aaaaaatggc aatttaatct ttcctgtaaa aatttgggta
     3061 ctttgccata cttatagtca aaccatactt tagtgataat taaatcggta tcgaaaaaaa
     3121 caaatttttt cccatcgctc ggatgatttt cataaaacag ttcttcatca atctgatggg
     3181 cggcaatgcg acaaacatcc tcatattcgt acggccggga taaactttcc acgtatgcgc
     3241 gagcaaattc aggtacccaa tccacctgaa aatattcggc aagggatttg gcaagctcgg
     3301 tttttccggt agattcagga cccaaaatag caactctcag catattcttc atttacttca
     3361 aatacatttt aatgtgtctg atgtttcatg gactttttcc actgaaaata gcccaacacg
     3421 gccatcagcg tataaacagt aaaaagtacg gctgtaggat aaagcccgcg ataaagatac
     3481 aaacctgttg aaacggcatc agcaataatc cacagtaacc aattctcgag caattttttt
     3541 gcaagcatcc acgtagccac tatactcaag gctgtggtaa aagcatcacc tctgggaagt
     3601 ggtgaatcgg taaaattact taaaatgaaa aaataaatca ccaaaattgc caaaaaaacc
     3661 gtcagcaaat tgaaagccat gtgtttggaa gttcgtgtta ccggcaattc gggttgggta
     3721 tccgtttttc ctcttttcca agtaatccat ccgtaaatgc tcactgcaag gtaataaaat
     3781 tgaagcgtca tgccggcata aaatttactt tgaaaaaaaa catatacgta caatgccgca
     3841 cacaaaaatc cgaaaatcca cagcccgatt ttctgcctca ccgataatat cagatatatc
     3901 aggctcaaaa tagcgccggt gagttctacc caattgtcga taacatattc tatgacagga
     3961 ttcaacgaaa aagtttcttt caacaaaatc attgctttga cctttttact ttaaaatttg
     4021 ttcgtacgca ttcggcgtct gcaataattc gactgctttt ttcagcactt cgttgtcgtt
     4081 gtttataatg cggtaatagg cgttcatatc ccacaaatcg cgtgcaatca atgccttaag
     4141 ttgcagtttg atcagctctc tcgatgtgtc aaactgagag gactggaacg gaatattttc
     4201 cttttctccg gcctgaatca gttgctgcaa aaaggcttcg tcaaccgtga attccttttc
     4261 aaatttgtcg aacgtactaa attttctctt caaatcctcc ctgttctgat cgataaactg
     4321 aacggaaact ttattgatta ctccccttgc aaccaacttt tgatgataat cggtaaaacg
     4381 tgtggtatcc agcggaacga aaacatcggg cataatacct ccaccgccat aaacggtacg
     4441 tttaagtcgc aaagtctgat aacggagtga atcggggaaa tgaatgctgt cgggatggat
     4501 taattctcct tttttgtaac gggtcagcaa ttcgcgctca tatttatcag caccatcttt
     4561 ataaggtttc tgaatgcaac gccccgatgg cgtataatat cgtgctgtcg tcaacctcat
     4621 catcgagcca tcaaccaacg gaagctgttg ctgcaccaat cctttgccga aagttcttcg
     4681 tccaacaatg actgctctgt cccaatcctg caaagctccc gaaacgattt cgctggctga
     4741 agccgagtat tcgtcaacca gaataaccag ttttccggtt tcgtattttc ctacggcggt
     4801 agcaagcgac tctttcttgg gttgatggaa tccctctgtg taaacgataa gttttccacg
     4861 atccaaaaat tgatcagcca gttgaatagc cgtagccaga taaccgcccc cattcccctg
     4921 caagctgaca atcaaatgct tcattccttt tttctgaagt tcggtaaatg ccttgacaaa
     4981 ttcttcatcg gttgtccgac caaaactgtt gattttgagg tatcctatat cattcccaat
     5041 catatactga gcatccacgc tgtagagggg aattttatcg cgtataattt tgaagatgag
     5101 caattcgggt tttccacgcc gcaaaacttt gactgtaact tccgaacctc gcggcccacg
     5161 caagcgtttc aaaatgtcgg aattttgaat ttttacaccc gccaccaatt cgtcgttaat
     5221 ataaataatt cggtcgccgg gaagaattcc tactttttcg gccggacatc ctgcaatagt
     5281 ttgaaccacc atcaaggtgt cttcaaaaat ttgaaactga atgcctattc cttcaaactc
     5341 gccttccagc ggttcattga tacgctccac ttcttcacgt ggaatgtagg atgaatgcgg
     5401 atccaagtcc ttcaacaggg cagttatggc cgattcgata agttttttat cgttaatgct
     5461 gtccacatac aagctggata tggcattcaa ggcattcatt aattttcgtt gctgcatcaa
     5521 cgatatgccc tgcccgaaaa ctccggcaag aattaaattg aaaataaaaa ccaaatataa
     5581 ctttttcatt ttttgttggt tatgtcataa gtaaaaaaga gaacaataat aatcaaacaa
     5641 tttttgtttc aaagaattaa tttttttcgg tttatctctt tgaaaattgc aactttagct
     5701 gaatcgattc aaatattctt caaatgaagc aatttctttc ccgcattcaa attttcgggc
     5761 cgacaatttt taatttctgc aatttctcga taaaactgtt caaccggcaa taaactttcg
     5821 tctgtttcca cataaagtct gtcaattggc gttactgcca cgcttgccgc attccgtttt
     5881 tcgccaaatg aaagagaaat tccggttttt aataattgct tggccaattc gggctttccg
     5941 cggaagccat gcacaatcca aagttgttcg ggtcgaaact tgttcctaat ttccaacaac
     6001 tcgttgaaat aaccgacaca atggataatc aaaggttttt tcaaactttc ggaataagaa
     6061 atttgacctt caaaaatgct tttctgcgtg gcaaaaggag tggaaatatt tttgtcgagt
     6121 ccacattcac ctatcgctat ccaccgtgta tcggaagaat aacgaaacaa cctttccaaa
     6181 tcttcaagat tttcctgttc tgcattccaa ggatgaatac ccaccgaaca aaaccgccgc
     6241 tcttcagtat caaaaaaaag agctgcctcc ttaatctgta aacttctcac agaatacgga
     6301 ttaaattcag atttcccatg tgtatggata tcaacaaaaa tgttcatctt tatcggcctc
     6361 tatgaactgc ggctaccttt gaaaacattg atgcaaaagt aaagaatttt caagtaaaat
     6421 tcatatcttt gcaaactttt cattagattt aaaggatatt tgaacaaaag aatcaacaaa
     6481 atgacagaca ataaagcatt gttcacttct ttgccgtaca aggtggccga tatttcgttg
     6541 gccgattttg gcagaaagga aatagaactg gcagaaaaag aaatgccggg acttatggca
     6601 attcgagaaa aatatgcagc ccagcagcca ctgaaaggtg cccgcattac cggctcactt
     6661 cacatgacta tccagacggc tgttttaatt gaaacgttag caaaacttgg cgctgaagta
     6721 cgctggtgca gttgcaatat ttattccacg caggatcatg ccgctgcagc catcgccgca
     6781 gcaggaatat ctgtttttgc ttggaaaggc gaaacactgg aagattactg gtggtgcacc
     6841 ttgcaggctt taacttttga aggcggtaaa ggcccgaatt taattgttga tgatggcggc
     6901 gatgcaacgc ttatgattca tacaggtgtg gcagcagaga aagatgcaaa cgtattgaac
     6961 cggcctgccg aaggagaaga tgaaaaagaa ttgttggcta tattgaaaaa agttttaaag
     7021 gaaaatagca acttctggtc gaatataact ccggaaattc gtggcgtttc ggaagaaacc
     7081 actacgggtg ttcatcggct ttatcagatg cttgcggaag gaacattgct gttccccgct
     7141 ttcaatgtca acgactccgt taccaaatcg aaatttgata atctttatgg ctgccgtgaa
     7201 tctttggccg acgggataaa aagggcaaca gatatcatgc tggcaggcaa agttgtggta
     7261 gtttgtggtt atggtgatgt ggggaaaggt tgtgccaaat ccatgcgggc ttatggagca
     7321 agagtgctgg taaccgaaat tgacccgatt tgtgctttgc aagctgcaat ggaaggattt
     7381 gaagtgtgta cggtagaaga tgctttgcct gaagggaata tttatgttac tgccaccggc
     7441 aatcgcgata ttattaccat tcaacacttg gaaaaaatga aggatggcgc tattgtgtgc
     7501 aatatcggac atttcgacaa cgaaattcag gtggatcagt tgaaaaaatt tcctggcatt
     7561 cagaaagtaa acatcaaacc gcaggtggat aagtatattt tccccgatgg acacagtgtt
     7621 ttgcttcttg ccgacggacg gcttgtaaat cttggctgtg caacaggtca tccttctttc
     7681 gtcatgagca attcctttac caaccaaaca ctggctcaaa ttgagctgtt tacaaaaaaa
     7741 tacgaaataa atgtttatcg tttacctaaa catttggacg aagaagttgc ccgactgcat
     7801 ttaggccaat tgggagtaaa gcttacccaa cttaccaaag agcaggcaga atatattggt
     7861 gtgaatcccg aaggaccttt taaaccggaa cattaccgtt actgaaatgt ttaaaaaatt
     7921 aatcgtattt tccacgaaga tattgttgtt ttcctttatt ttcaattcat tctttttaac
     7981 tcaattacct ttttctacaa acaaactaaa aaccgtaaaa ataagaaacc cacatgtatt
     8041 gcatacacaa acacgaatga atataatatt cagacaggtg ggttccatca gacctttaaa
     8101 aaataaatta tattctgatg aaattgatat gcctaaaaac atttaatcaa gttataacat
     8161 gtataccctt ttggatggaa aactaatttc ggaacagata aaaaaggaaa ttgctgaaga
     8221 agttcaacaa atgataaaag ccggtaaaaa gcgtcctcat ctggcagtga ttattgttgg
     8281 tcatgatggt ggaagtgaat cgtatgtagc cggaaagatc aaagattgcc aaatctgcgg
     8341 atttcagtcc actctgattc gattagaaga agatgtaaca gaacaacgtt tgctgcaaga
     8401 agtggaacgt ttaaatgaag atactgatgt tgatggattc attgttcaat tacctttacc
     8461 gaaacatatt tcggaacaaa aaatgattga agcaattaat tataaaaaag atgtagatgg
     8521 ttttcatccg atcaatgttg gccgattatg tataggatta ccgtgttatg tttcggccac
     8581 gccaatggga attatggaat tattgaagcg ttatcggata gaaaccagtg gaaaaaactg
     8641 tgttgttgtt gggcgcagta acatagtagg taagcctact gccatgcttt tgatgcaaaa
     8701 aacttatccg ggtgatgcaa ccgttacgat ttgccacagt cataccaaaa acttgaaaga
     8761 aatttgccaa caagccgata ttatcgtggc agccataggg caaccagagt ttttaaaggg
     8821 ggatatggta aaagaaggag cagtggttat tgatgttgga acaacaagag ttccctcaac
     8881 cgaaacaaaa tcgggtttca aactaaaagg agatgtagat ttcaatgaag tagctccaaa
     8941 atgttcattt attactcctg tgccgggagg tgtaggatta atgactcgcg tttcgcttat
     9001 gaaaaacacc ttgttggctg ccaaagccga aatttatgag aaaaatggat aaatataatt
     9061 tttttcggat aaatttggtt caacttatat tgcctgtttt gacacaataa attttatcgt
     9121 tactttttga taattatgac cgttttttat gctcccgaag ttgcttttaa caatgtttta
     9181 cccaaagaag aatcacaaca tgccataaaa gttcttcgct taaaaaaagg agataaaata
     9241 gtgatagttg atggaaaggg gaatggctac cttgctgaaa ttcttttccc agacagtaac
     9301 cattgcgaat ttgaaattct tgagactttg cctgatgaag gaaacgttca ttatcattta
     9361 cacattgcca tagctcccac taaaaatgtc gagcgttttg agtggtttgt tgagaaagca
     9421 accgaaatag ggatacatga aattacccct attatttgta agttttccga gcgaaagatg
     9481 attaagcccg aacgacttga gaaaattgtt atttctgcct gcaagcaatc aatgagagca
     9541 caatttcctg tcgttcattc cctttgcagt tttcaggaag tactgacaac ctactcggca
     9601 cagcaaaaat tcatagcaca ctgttatacc gaccagataa tgaaagaatt tgactgtgtg
     9661 agaaagagaa attttttaaa aaatctgata gaaccatcca cgaaaatatt tattttgatt
     9721 ggtcctgaag gcgacttcag tatcgacgaa gtaaaaacag ccatcgaaaa aggattcgat
     9781 cctgtttctc tcggagtggg gcgtttaaga acagagacag ctggaattgt ggcctgcacg
     9841 accgtagcgc ttatcaatca ataaatttat attgggacaa ttatattctc tcgtaaattc
     9901 aagcaattaa acccaactcc acaataggaa aaaaggtttt aatcttgtat aaaacagata
     9961 aaattttatc taccatataa aacttttatg cagaacttct ccagtagctg gatataaaaa
    10021 aatcagcagg gctgtttaac aaaattgctt tcaataaaaa aacgagcttg gttttcctga
    10081 ctatcgaaca actcttccga ctttgacttt ccctttgaat tggctaatga gtaaatctct
    10141 taaggacgaa actatttgta gcccatgccc aaatgtgaga gaaatttatc ggaacaaatt
    10201 ttttctggat ttatccgctt actttgtttc ttcttcaatt tcttcaaaat caacgtattc
    10261 tccatcgttt ttgtcgaaaa tttttttctt ctttgatgaa tgtgtaaaaa tgttgttatc
    10321 ttccttagat tgttttcttt ttttactttt tctcccaaaa ttaaaaaacg accgaatgaa
    10381 tcccaacaca aaaagaagga aaagtactcc aataaagaca ataagaatca gtatggaaaa
    10441 aataagatgc ataaatagag attgaatatt ttaaaaaacg gctttagcaa cacgcttaac
    10501 actctgtgca gccattaagc tgtaaaaatg aatacttggt acaccatgag cctttaaatc
    10561 ggaggcctgc ttggtacacc attccacccc cacttcggtt gcctctgcat cggttttaca
    10621 cttgcgaagt tcagacgcaa actcttcagg aatttcggtg tgaaaaattt taggcagcac
    10681 tgtgagctga ttcattaaag ttattggttt tatacctgga ataataggaa cggtaatccc
    10741 catgtttctg cacttttcta caaaatcgta atacttttgg ttgtcgaaaa acatttgggt
    10801 aaccagatat tcggctccgc catccacttt cattttagta taatacaagt ccgattcggt
    10861 gttgggcgat tcttcatgtc tttcaggata ggctgccatt ccataggaaa aaggctctgc
    10921 gggaagtgct ttcattctgc tgccatccag aaaaataccc ttgttgaagt tgttgacttg
    10981 ttgctgaaga tcggtggcat gcatatgtcc cgttggaaca aaatccttct catgcttggc
    11041 tttatctcct ctgagcagca aaaggtcagt gattcccaaa aattgcaaat caataagttc
    11101 atattcaatt tcttcctttg taaatccact gcatatcaca tgcggaacaa ccggaatttt
    11161 atatttactt tttatggcag cggcaatagc gaccgtccca ggacgccgac gaacatctga
    11221 ttgttcgaaa agtccctgat cgttttgttt gaaaataact tcgctccgat gcgttgtaat
    11281 attgatatat ttaggatcga attctcttag taaatcgatg gttttaaata cggaagctat
    11341 gccatcccct ttcaatggag gcaaaagctc gaaagaaaat gctgtggaag aagaactttt
    11401 tattttgtcg acaatactca tatcttaaaa ggaaacgtaa gaaacttaca ataaaaaaac
    11461 gaggaataaa gaaatttaat ttgcaaaatt acatgaaaat cgaggtattt ccaaacgtgt
    11521 gaaaataaaa atcaaggcca caaaacttga aaggtttacc gtaccgtgct cgaagcacaa
    11581 atgttgcttt tgtaaattgg ttttttaacg accaatgaaa taaactaatt gttgaacaat
    11641 ttaatccggt aattttatgg aaaaagcatg tggccttgac gttcacaaag atagcatatt
    11701 tgcatgcatt ttggacgaac aaggcaaaaa aattttagaa aaacgttacg gaacactcac
    11761 tgccgattta atgacccctt tcgtgattca ctgatagaac aaggttgcgg aaaagtagcg
    11821 atggagagta gcagcattta ttggataccg atctggcagg ttcttacatc ggatttttct
    11881 ttaaaactag ccaatcccta tttcattaaa acagctgccc ggtcgcaaga gtgacgtaaa
    11941 ggatgcacag tggatagcgg aatgtcttca gaaagatttg ataaagggca gttttgttcc
    12001 tgaagggatt ttacaacaga tgcgtcaact cagccgaca
//
LOCUS       DAOTAX010000058        10852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_5172_length_10852_cov_29.146430, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000058 DAOTAX010000000
VERSION     DAOTAX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5172_length_10852_cov_29.146430"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLM04_06720"
     CDS             <1..1314
                     /locus_tag="PLM04_06720"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HON02802.1"
                     /translation="WKASGHVDAFNDPLIDNKDSKKRYRADVLIEELIAKYDEKIEKE
                     VEKAAKRFGENFDENLFRATNERVKEHQAKRDEIHRRYVKSLNDNDLAELRAIIIDNE
                     IVCPVSGTKNWTEVRQFNLMFTTEMGSNADSAMKIYLRPETAQGIFVNFLNVQKTGRM
                     KIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPGEEMKWFEYWKAYRMKWH
                     KALGLGDHKYRFHDHDKLAHYANAATDIEFELPFGFKEIEGIHSRTDFDLKNHEKYSG
                     KNLKYYDAELKESYTPYVIETSIGVDRTFLSIMASAYTEEKLEDGETRVVLKLPPVLA
                     PVKIGIFPLIKKDGLPEVAHKIFEDLKFDFKCFYDDKDSIGKRYRRQDAIGTPFCITV
                     DHQTLEDETVTIRYRDSMKQERVKIEALSAILNEKGSMKKLLKQL"
     gene            1630..3126
                     /locus_tag="PLM04_06725"
     CDS             1630..3126
                     /locus_tag="PLM04_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HON02803.1"
                     /translation="MELAVEFYFLVASVLLILSIVAGKTGYRFGVPALLLFLLIGILA
                     GSDGLGIEFNSPKQAQLVGVVALNIILFSGGMDTRFSEIKPVLWQGIILSTFGVLLTA
                     FITGCFIYWLTNHLFTAITFSLLESMLLGSIMSSTDSASVFSILRSKKLSIKHNLRPL
                     LELESGSNDPIAYLLMIIFIQLLQSSDISSGEVVAFFFRQFIIGAICGYILSKMVIFI
                     INRIRLDYEGLYSVLLLSFLFFLYSFTSILGGNGYLAVYIGGLFIGNQRFVHKKSSLK
                     FFDGLTWLFQIIMFLTLGLLVNPHELLPIAGAGILIGLFMIVISRPISVFILLSPFRK
                     INFKSKLFVSWVGLRGAVPIIFATYPLMSGIPQARMMFNIVFFITILSLVLQGSTIPF
                     VARLLKLSMPSKDKPKLKEFDIDLPEDIKSAISEITVTEEMLQKGRKLMDITLPDQTL
                     AVMIKRNDHFFVPRGNTKLEIGDTVLLIADNDEIMKETFKKLGVDKEF"
     gene            complement(3155..3610)
                     /locus_tag="PLM04_06730"
     CDS             complement(3155..3610)
                     /locus_tag="PLM04_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HON02804.1"
                     /translation="MKVEIFCPITNKITDESVARLNAGFTVVLLLSYFVTSNIVPVVF
                     LLLDFTARAIERPEWSILSKTSKWILKKLHVESKLINAGPKIFAARIGSFFSLITILF
                     FLLKLPSISIALAAVFFVFALLESAFNYCVACRIYPFIYHALQAKDKEK"
     gene            3798..4550
                     /gene="lptB"
                     /locus_tag="PLM04_06735"
     CDS             3798..4550
                     /gene="lptB"
                     /locus_tag="PLM04_06735"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HON02805.1"
                     /translation="MSNDEMVLRTEHLVKRYGKRTVVDDVSIEVRQGEIVGLLGPNGA
                     GKTTTFYMVVGLVTPNAGKIFLNDIDITKYPVYKRAQSGIGYLAQEPSVFRKMTVEDN
                     IKSVLEMTKFTKEYQKKKLETLIEEFNLEHVRKNIGDRLSGGERRRTEIARCLAIEPR
                     FILLDEPFAGVDPIAVQDIQDIVWKLKDKNIGILITDHNVDETLAITDRAYMLFDGRI
                     MFQGSALELSENPIVREKYLGRDFELKKKTKV"
     gene            4617..5531
                     /locus_tag="PLM04_06740"
     CDS             4617..5531
                     /locus_tag="PLM04_06740"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HON02806.1"
                     /translation="MPKATFTILGCGSALPTKKNFPSSFLLDLREKQFLIDCGEGAQI
                     RLRQMGLKTNRLGHIFISHLHGDHCLGLLGLISSFSMLHRTADLHIHAHPDLEKIMDV
                     PLHYFSAETPFQLFFHPFNPQVNELIYEDRTVKVSTIPLKHKVPTCGFLFEEKPLERH
                     LIREMIDFYRIPVSQLHKIKQGEDFVTEDGEVVPNERLTYENVPPKRFAYCSDTAYDE
                     SIVPIIRQVDCLYHEATFLEEDIVRADKTLHSTALQAAEIAKKAEVGKLIIGHYSARY
                     ADQSVLLNEAASVFQQVIAGQDMGVYEI"
     gene            5575..6252
                     /gene="trmD"
                     /locus_tag="PLM04_06745"
     CDS             5575..6252
                     /gene="trmD"
                     /locus_tag="PLM04_06745"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HON02807.1"
                     /translation="MRIDIISAVPQLLESPLNHSIVKRAQEKHLVEIFVHNLHDYSYD
                     KRRNVDDYAFGFSAGMVLRIEPIDRVISKLKQERNYDEIIYTSPDGEMFNQKIANQLS
                     LCENIIILCGHYKGVDQRVRDHLITREISVGDYVLTGGELPAAIMTDAIVRLLPGAIG
                     DETSALLDSFQDNLLAPPVYTRPADYKGWKVPDVLLSGHEANIRQWLHAQSVERTRRR
                     RPDLLEE"
     gene            complement(6337..7089)
                     /locus_tag="PLM04_06750"
     CDS             complement(6337..7089)
                     /locus_tag="PLM04_06750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HON02808.1"
                     /translation="MKLKGKVAIVTGGARDLGRAVSIKLASEGAKVVVNYFDNLEDAK
                     ETLKMIQENGGEGIIVQGDMTKAEEVKKLFDAGIAAFGNRIDILVNVVGGICGRKEIT
                     EQDEAWYNLLMDVNMKSCWLCTREVVPHMTNGGAIVNFSSLAARDGGGQGASMYASAK
                     GAVMTFTRSMAKELGPKGIRVNAVAPGTIATSFHDRFNTPENREKMKAIYPLRREGDA
                     GDIADLVLFLVSDDSDYITGTNIDINGGIFFS"
     gene            complement(7111..8574)
                     /locus_tag="PLM04_06755"
     CDS             complement(7111..8574)
                     /locus_tag="PLM04_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02809.1"
                     /translation="MKIKGLRWWIIGLIMVITIINYLDRGTLNYMWVTNVEYSIVDQF
                     STNTKNYEAVFLKEKNSYLLKNKKGETAEVISSHLLEKKDKKVVVNREGIAYDLGIID
                     KNTTPDEASKKAKGILGTITIFFMIAYGISQLFSGKMYDKIGTRKGFVISAAIWGMAD
                     ALTSLAKGKFSLTAFRMMLGLGEAGPWPGAVKSNAEWFPQKERAFAQGLFNAAASAGS
                     ILAPIIILMLYLLLGWRLTFLIVGSLGLIWIIPWLIINKKGPKDHPWITEKEREYILT
                     GQPEAQHVNEKGKSWKELLSNKKNYSVILGRFFLDPIWWMFVTYLPLYLVEQFNLNIT
                     ELAFSAWVPYFGAMIGSLTGGWFSGYLIRSGKTVDKARKIAMIVGGLIIIPSVIASVL
                     ASNAILAVVLMAFVLGGFQFTMTNIQTIPSDLHSGPSVGSLAGLGGAAAVLGTILAIL
                     FSAQITSWPLLFGLLAALVPLSLGSIFLTVGKIEHIK"
     gene            complement(8579..>8791)
                     /locus_tag="PLM04_06760"
     CDS             complement(8579..>8791)
                     /locus_tag="PLM04_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HON02810.1"
                     /translation="AVGYIHEHFHSQTTYVASGKFEVSINGEKQILEAGDGFYIEPDA
                     PHGAICLEDGILIDVFSPMRMDFLKI"
     assembly_gap    8792..8891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8892..10810)
                     /locus_tag="PLM04_06765"
     CDS             complement(<8892..10810)
                     /locus_tag="PLM04_06765"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HON02811.1"
                     /translation="MIFIGLSALISASGQHPNLLLTKQGVKEIQASLGKYANFDKSVN
                     ELKAIADKALNSEIIVPLPLNEGGGYTHEKHKNNYYEMNAAGTMYQISGKSEYAEFVR
                     KMLMKYAELYPTLGLHPSKRSETPGKIFWQLLNDCVWLFHTALAYDCIYDYLNSSQKT
                     NLEKNLFRPMAEFISNGSPANNEVFNKMHNHGTWATASVGMIGYVMGDKNLVEKALYG
                     SNKDNKSGFIRQLDVLFSPDGYYTEGPYYQRYAIWPFMVFAQVIENNQPELKIFSYRD
                     SVLLKATNTLIQCAYNGNIFYLNDALKKNYKTQEIVYAVDIAYKNNPKNTFLLDIAQQ
                     QKSFIISDAGIATAKSLNIVKYEPFQFRSVLLRDGANGDEGALGIIRAGKKDKTQMCL
                     TFKATSHGLSHGHYDKLSISMYDGGNFVLTDYGAVRFLNIEPKSGGNYAKENHSWAAQ
                     TIAHNTVTVDETSNFNGNIKVSSLHHSDIQYYDFSSNQIQIISGCENNAYKNVKMQRT
                     VAVIENKTFSYPIVIDIFRIISDSTHTLDFPFYYRGQMISTNIQYQKFTAQLNPLGTK
                     NGYQHLWVEAIGKNEKPVTQFTWVEGNRFYTITTLGNSNTEYLITRTGAGDPNFNLRN
                     ETAFMMRQPMVKKHT"
BASE COUNT         3200 a         2009 c         2099 g         3444 t
ORIGIN      
        1 tggaaagctt cgggacatgt ggatgctttc aatgatccgc tgattgacaa caaagacagt
       61 aaaaagcgtt atcgtgccga tgtgctcata gaggaattga tagccaaata tgatgaaaaa
      121 atcgaaaagg aagtggaaaa agcagccaaa cgatttggcg aaaatttcga tgaaaacctc
      181 tttcgggcta ccaatgagcg ggtgaaggag catcaggcaa aacgtgatga aatccatcgc
      241 cgatatgtta aatctttaaa cgataacgat ttggcagaat tgcgagcaat tattatcgac
      301 aatgaaattg tttgtccggt aagtggaaca aaaaactgga cggaagtgcg ccagtttaat
      361 ttgatgttca ctaccgaaat gggttccaat gccgacagtg ccatgaaaat ttatttgcgt
      421 cccgaaactg cacaaggtat ttttgttaat tttctgaatg tccagaaaac cggaaggatg
      481 aaaattccct ttggtattgc acaaatagga aaagccttcc gaaatgaaat tgtggcgcgt
      541 cagtttattt tccgaatgcg ggaattcgag caaatggaaa tgcagttttt tgttcggccg
      601 ggtgaggaaa tgaaatggtt tgaatattgg aaagcatatc gaatgaaatg gcacaaagct
      661 ttgggcttgg gcgatcacaa atatcgtttt cacgaccacg acaagctggc acattacgcc
      721 aatgctgcta ccgatattga atttgaattg ccttttggtt ttaaagaaat tgaagggatt
      781 cattcccgta ccgatttcga tttgaaaaat cacgaaaaat attcggggaa aaatttgaaa
      841 tactacgatg cggaattgaa agaatcttat actccctacg tgattgaaac ctctatcgga
      901 gtggacagaa catttctttc cattatggct tctgcttata cagaggaaaa actcgaagat
      961 ggtgaaacgc gtgtggtgtt aaaacttccg ccggtattgg ctcccgtaaa aatcggaatt
     1021 tttccgttaa ttaaaaaaga tggactgcct gaggtggcac ataaaatttt tgaagatttg
     1081 aaattcgatt ttaaatgttt ttatgacgac aaagattcca ttggaaagcg atatcgtcgt
     1141 caggatgcaa ttgggacacc attctgtatt acagttgatc atcaaacgct cgaagatgaa
     1201 accgttacca ttcgttatcg ggattccatg aaacaggaac gtgtaaaaat cgaagcatta
     1261 tctgctattc tgaacgaaaa aggcagtatg aaaaaactct tgaaacaact gtaaaaacgg
     1321 attaaaggtt aattccatca gaatataatt tatttttatg ttctgaggaa ctcacatgtc
     1381 tgaaattata ttccttcgtg tttgtgtatg taatacatgt gggtttctta aaaaattatt
     1441 tgtttttcta cacaaaagcc acgataaggt agtaaaatct tgaagaaaag ttttttccat
     1501 atttttttaa aatcgatgaa ccccatttgt atttagtagg tttttggtac acaaaccaac
     1561 ttgtttgata gacgacttta attctttgaa aaaaatctta agtttgttag aacatcaacg
     1621 aattatgaaa tggaacttgc agttgaattt tattttctgg ttgcttctgt tcttcttatt
     1681 ttaagtattg tagccggtaa aacgggttat cgttttggag tcccggcgtt gttgcttttt
     1741 ttgcttatag gtattcttgc cggaagcgat ggattgggga tagagtttaa ttctcccaaa
     1801 caagctcagc tggttggggt ggtggctttg aatattattt tattttccgg cggtatggat
     1861 acccgttttt cggaaataaa accagttctt tggcaaggca tcattctgtc cacttttggc
     1921 gtattgctga ctgcatttat tacaggatgt ttcatctact ggttgacaaa tcatcttttc
     1981 actgccatta ctttcagctt attggagtcg atgctcttgg gcagcatcat gtcttctacc
     2041 gattcagctt cggttttttc tattctgcgt tccaaaaaat tgtccatcaa acacaacttg
     2101 cgtcctttgc ttgagctgga aagcggtagc aatgacccta ttgcttatct gttgatgatt
     2161 attttcatac aacttttaca atcttccgat attagttcag gtgaagtagt agcctttttc
     2221 tttcgtcaat ttatcattgg cgcgatttgt gggtatatct tatcgaaaat ggtcattttc
     2281 attatcaatc ggattcgatt ggattatgaa ggattatatt ctgtactctt actttcgttt
     2341 ctgttttttc tttacagttt tacttctatt ttgggcggaa acggttatct ggctgtatat
     2401 attggagggc tttttattgg caatcagcga tttgttcaca aaaaaagcag ccttaaattt
     2461 tttgatggac taacatggct gtttcaaata attatgttcc taacgcttgg gcttttggtc
     2521 aatcctcatg aacttcttcc catagctgga gcagggatat tgattggatt gttcatgatt
     2581 gtaatttcgc gtcccatttc ggtttttatt ctactttctc ctttcagaaa gataaatttt
     2641 aaaagtaaac tttttgtttc atgggtcggg ttgagaggcg ctgtaccgat aatttttgct
     2701 acttatccct tgatgagcgg aataccgcaa gccagaatga tgttcaacat agtttttttc
     2761 atcacaattc tttcgttggt gcttcaaggt tctaccattc cttttgttgc acgtctgctg
     2821 aaattatcca tgccttccaa agataaaccc aagttgaagg aatttgacat agatttgcct
     2881 gaggatatta aatcggccat aagcgaaatc actgttaccg aagaaatgtt gcaaaaaggt
     2941 cgtaaactga tggatatcac cttgcccgat caaacgctgg cagtaatgat taagcggaac
     3001 gatcattttt ttgttcctcg aggaaatacc aaactggaaa tcggagatac ggtactgttg
     3061 attgccgata atgacgaaat catgaaagaa acattcaaga aactgggtgt agacaaggag
     3121 ttttagaaaa gtttcttcat tcctgatatt tttttcattt ctctttatct tttgcttgta
     3181 gagcatgata aatgaaagga taaattctgc aggctacaca gtaattaaat gccgattcca
     3241 acaaagcaaa gacaaaaaat acggctgcaa gggcaatgct gatggatggt aacttcagca
     3301 agaaaaacag aatcgtgatg aggctgaaaa agctgccaat gcgtgctgca aaaatttttg
     3361 gtccggcatt gattaatttt gattcgacat gcaacttttt taatatccat tttgacgtct
     3421 ttgataaaat actccattcg ggtcgttcta tagctcgagc agtgaaatca agcaacagga
     3481 aaactaccgg gacaatattt gaagttacaa aataagacag taataacaca actgtaaacc
     3541 cggcattcaa tcgggcgacg ctctcatcag tgattttatt tgtaatcggg caaaatattt
     3601 caactttcat gatgtttttt tattagaatt tatacagcaa aattacttct ctttcaactg
     3661 cacgtaaata ccattgaaaa tgtattttat ctaccacaat ttaggtacag catccaacaa
     3721 aggattgctg caatcgaaaa aaattcgttt gattgtcaag ataaaaaagt gtaaatttgc
     3781 aacttcaaaa aatagttatg agcaatgatg aaatggtgct gcgcaccgaa catttggtaa
     3841 aacgttacgg caaaagaacc gtagtagatg atgtttccat tgaagttcgg caaggtgaaa
     3901 ttgtaggttt gttgggaccg aatggtgccg gcaaaacgac tactttttac atggttgtgg
     3961 gattggtaac gcctaacgcc ggtaaaattt tcttaaacga catcgatata acaaaatatc
     4021 ctgtttataa acgcgcacaa agcggtatcg gatatttggc ccaagaacct tccgttttca
     4081 gaaaaatgac ggtagaagac aatattaaat cggtgctgga aatgacaaaa ttcactaaag
     4141 aatatcagaa aaaaaaattg gaaacattga tagaagaatt taatctggaa catgtgagaa
     4201 aaaacattgg tgaccgtttg tcgggtgggg agcggagaag aaccgaaata gcacgctgtt
     4261 tggctattga accgcgtttt attttgcttg acgagccgtt tgccggagtt gatcctattg
     4321 ctgttcagga tattcaggat attgtttgga aattgaagga taaaaacata ggtattctga
     4381 taaccgatca caatgtagat gaaacattgg ccatcaccga tcgtgcctat atgcttttcg
     4441 atgggcgaat catgtttcag ggctctgctc ttgaattgtc cgaaaatccg attgtccgtg
     4501 aaaaatatct gggacgcgat ttcgaattga aaaagaaaac aaaagtttaa attttctggt
     4561 tcaagcgtta aagcaaaaaa tgccaaaaga ttttttcttt ttttgaaaaa tcaatcatgc
     4621 caaaagcaac gtttactatt ctgggttgtg gttcggcttt gcctaccaaa aaaaattttc
     4681 caagttcttt tttgctcgac ttgcgcgaaa agcagttttt gattgattgc ggagaaggtg
     4741 ctcaaatcag acttcgacaa atgggactga aaaccaatcg cttgggacac atttttattt
     4801 ctcatctgca tggtgatcat tgtctgggtt tgttgggact gatttcttct ttttccatgt
     4861 tacatcgtac agccgatcta catattcatg ctcatcccga cttggaaaaa ataatggacg
     4921 ttcctttgca ctatttttct gctgaaaccc cctttcaatt gtttttccat ccattcaatc
     4981 cacaagtgaa cgaactgatt tatgaggatc gtacggtaaa agtttcgacc atcccgctga
     5041 agcacaaggt gccaacttgt ggttttctgt ttgaagaaaa accgctcgaa agacatttga
     5101 tacgagaaat gatcgatttc taccgtatcc ctgtttcgca gttgcataaa atcaagcagg
     5161 gagaggattt tgttacggaa gatggggaag ttgttccgaa tgagcgacta acttatgaaa
     5221 atgtccctcc caagcgtttt gcctattgtt ctgatacggc ttacgacgaa agtattgtgc
     5281 ccattattcg tcaggtagat tgtctgtatc atgaagctac ttttttggaa gaagatatag
     5341 tgagggcaga taagacgctt cattccacag cacttcaggc agccgaaatt gcaaaaaaag
     5401 ccgaagtggg gaaattgatt atcggacatt attcagcgcg ttatgctgac cagtctgtgc
     5461 ttttgaatga agctgcttcc gtttttcaac aggttattgc agggcaggat atgggcgtgt
     5521 atgaaatata aagggaaagc tcgaatgtaa aatgattatc gatttatggg aaaaatgcga
     5581 attgatatta tttctgctgt tccacaattg ttggaaagtc cattgaatca ttccattgta
     5641 aaaagagcac aggaaaagca tttggtggag attttcgtac acaatttgca cgattacagt
     5701 tacgataaac gacgaaatgt tgacgactat gcttttggtt tcagtgccgg aatggtattg
     5761 agaattgaac ctatcgatcg agtaatttca aaactcaagc aagaaagaaa ttatgatgag
     5821 attatttaca cttcgcctga tggagaaatg tttaatcaaa aaattgccaa tcagctttct
     5881 ctatgtgaaa atatcatcat tctttgcggt cattataagg gtgttgatca gcgggtgcgc
     5941 gatcatttga ttacacgcga aatcagtgtg ggagattatg tgctcacagg gggtgaactt
     6001 cctgcagcca tcatgactga tgctattgtc cgtctgttgc cgggtgctat tggagatgaa
     6061 acttcggctt tgcttgattc gtttcaggac aacttgttgg cgccgccggt ttatactcgt
     6121 ccggcagatt ataaaggttg gaaagttccg gatgttttgc tatcgggtca tgaagccaat
     6181 atccgacaat ggctacacgc acagtcggta gaacgaacgc ggcgacgtcg tcccgatttg
     6241 ctggaggaat aaattttctc attatgataa aaagctcctg atgaaaaaat gctttcaaca
     6301 agagcttttt ttaacacaat ctaaccatat tttttcttaa gagaaaaaaa ttcctccatt
     6361 aatatcaatg ttagttcctg tgatataatc ggaatcatcc gaaacaagga acaatacaag
     6421 gtcggctata tcgccggcat cgccttcccg tcgaagcgga tatattgctt tcattttttc
     6481 cctgttttcc ggagtattaa atctgtcatg gaaagaagta gctattgtac ccggagcaac
     6541 agcattcaca cgaatacctt ttggtcccaa ttccttagcc atggaacgag tgaaagtcat
     6601 gacagcacct tttgccgagg catacatgga agctccttgt ccgccaccgt ctctggctgc
     6661 gagtgacgaa aaattcacaa tcgcgccgcc atttgtcata tggggaacaa cttcgcgggt
     6721 acaaagccag caacttttca tgttcacatc cattaaaaga ttataccatg cttcatcttg
     6781 ctcggtgatt tcttttcttc cacaaatacc gccaactacg ttgaccagta tgtcaattct
     6841 atttccaaaa gcagctatac cggcatcaaa taattttttt acttcctctg cttttgtcat
     6901 gtcgccttga acgataatac cttcaccacc attttcctgt atcattttca aagtttcttt
     6961 agcatcttcg agattgtcaa aataattgac aacaactttt gcaccttcag aagccaattt
     7021 aattgatact gctcttccta aatccctagc tccaccagtt acgatggcaa cttttccttt
     7081 taatttcata attataatat ttttaattga ttattttatg tgttctattt ttcctaccgt
     7141 taaaaatatt gatcctaacg acaacggtac caacgccgcc agcaaaccaa aaagtaatgg
     7201 ccaactggta atttgtgcag aaaataaaat ggccaaaatg gttccgagta cggctgctgc
     7261 tccacccaat cctgctaaag aaccaactga tggcccactg tgtaaatcac ttggaatagt
     7321 ttgaatgttg gtcattgtaa attggaagcc acccaataca aatgccatta aaacgactgc
     7381 caatattgca ttggaagcca atacagaagc tattacggaa ggaataatta ttaatcctcc
     7441 aactatcatt gctatttttc tggccttgtc aacggttttt ccgctgcgga ttaggtaacc
     7501 tgagaaccaa ccaccggtta gacttcctat catggctcca aaatagggca cccatgcaga
     7561 aaaagccagt tccgtgatat tcagattgaa ttgttcaacc aaataaagtg gaagatacgt
     7621 tacaaacatc caccagatgg gatcgagaaa aaaacgacct aaaataacgg aataattctt
     7681 tttatttgat agtaattctt tccaactttt ccctttttcg tttacgtgtt gagcttcggg
     7741 ctggccggta agaatgtatt cacgttcttt ttccgtaatc cagggatgat cttttggtcc
     7801 ttttttgttg attatcagcc agggaataat ccagataagt cctaaagaac caacaatcag
     7861 gaaagtaagt ctccatccca gcaacaagta aagcattaaa attatgatag gagcaaggat
     7921 tgaaccggct gaagctgctg cattaaaaag tccctgtgca aaagctcttt ctttttgagg
     7981 gaaccactcg gcattgcttt ttactgcccc gggccacggg ccagcttccc ccaatccaag
     8041 catcattctg aaagcggtaa gtgaaaattt tccttttgcc aaagaagtta gcgcatctgc
     8101 cattccccag atagcagctg atatgacaaa gccctttcgc gtaccaattt tgtcgtacat
     8161 tttacccgaa aacaactggc taataccata ggcaatcata aaaaatatgg taatagttcc
     8221 taaaattcct ttagctttct ttgatgcttc atcaggtgtg gtatttttat cgatgatgcc
     8281 taaatcataa gctatccctt ctctgttcac cactactttt ttatcttttt tctccaataa
     8341 gtgagatgaa atcacttctg ctgtttctcc ctttttgttt ttcagcaaat aactgttttt
     8401 ttctttcaaa aaaacggctt cgtaattttt agtgttggtt gaaaactgat caactatgga
     8461 atattcgaca ttcgttaccc acatgtaatt caatgtacct ctatccagat agttaatgat
     8521 ggttatgacc ataattaatc caataatcca ccaacgcaat cctttgattt tcatatattt
     8581 atatttttaa gaaatccatc ctcattgggc tgaaaacatc gatcaaaatt ccatcttcca
     8641 gacatattgc tccgtgtgga gcgtcgggtt cgatataaaa gccgtctcca gcctccaaaa
     8701 tctgtttttc gccatttatc gaaacttcaa atttgccact ggctacataa gtagtttgtg
     8761 aatgaaagtg ctcatgaata taaccgaccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn naatgtgtgt ttttttacca taggttggcg catcataaat gcagtttcat
     8941 ttctgagatt aaaatttggg tcaccagctc ctgtacgtgt aatcagatat tctgtgtttg
     9001 aattacctaa tgtcgtaatc gtataaaatc tatttccttc aacccacgta aactgagtaa
     9061 ccggtttttc gtttttcccg attgcctcaa cccaaagatg ttgataacca tttttcgttc
     9121 ccaatggatt taattgagca gtaaattttt gatattgaat attggtagaa atcatttgac
     9181 ctctatagta aaaagggaaa tcaagtgtat gtgtagagtc ggagatgatt ctaaaaatat
     9241 ctatgacaat tggatacgaa aatgttttgt tctcgattac agctacagtt cgctgcattt
     9301 tcacattttt ataggcattg ttttcgcagc cggaaataat ttgtatttga ttacttgaaa
     9361 agtcataata ttgaatatcg gaatgatgaa gggaggaaac tttaatattg ccattaaaat
     9421 tacttgtttc atcaactgtt acggtattat gggcaatggt ttgtgcagcc caggaatgat
     9481 tttcttttgc ataatttcca ccgcttttcg gttctatatt gagaaagcgg acagcgccat
     9541 aatcggttaa aacgaaattt cctccatcat acatagaaat ggacagttta tcgtaatgac
     9601 catgtgataa tccatgagaa gttgctttga aggtaagaca catttgtgtt ttatcttttt
     9661 tccctgctct gataatgcct aatgctcctt catcgccatt agctccatct cgcaaaagaa
     9721 ctgagcgaaa ctggaaaggt tcatatttca caatattcag cgactttgct gtagcaatgc
     9781 ccgcatccga tatgatgaat gatttttgct gttgtgcaat atcaagtaga aaagtatttt
     9841 ttggattatt tttataggca atatcaactg cgtacactat ttcttgtgtt ttataattct
     9901 tttttaaagc atcgttcaaa tagaaaatat ttccattata agcacattga atcaaagtat
     9961 tggttgcttt taacaaaaca ctgtctctat aactgaaaat tttcaattcg ggctgattgt
    10021 tttcaatcac ttgagcaaaa accataaatg gccaaatagc ataacgttga taataaggtc
    10081 cttctgtata atagccatct ggagagaata atacatccaa ctgacgtata aatcctgatt
    10141 tattgtcttt gttagaacca taaagggctt tttcaaccag atttttatca cccataacat
    10201 atccgatcat acctaccgaa gctgtagccc atgtgccgtg gttgtgcatt ttgttgaaaa
    10261 cctcattgtt ggccggactc ccgttcgaga taaattctgc catagggcga aagagatttt
    10321 tttcaaggtt tgtcttttga gaggaattta gataatcgta aatgcaatcg taagcgagag
    10381 cagtatggaa tagccagaca caatcgttca gcaattgcca gaatatttta cccggtgttt
    10441 ctgatctttt ggatggatgc aatcctaaag taggatataa ttctgcatat ttcatcagca
    10501 ttttccgaac aaattcagca tattcgcttt tacccgaaat ttggtacatc gttccggctg
    10561 cattcatttc atagtaatta ttcttgtgct tttcgtgtgt atatccacca ccttcattca
    10621 atggcaacgg aacaataatt tcggaattca atgctttatc ggcgatcgct tttaattcgt
    10681 ttaccgactt gtcaaagttg gcatattttc ccagtgatgc ttgtatttct tttactccct
    10741 gcttagtcag cagcagattc ggatgttgac ccgaagctga tattaaagca gatagaccaa
    10801 tgaatatcaa tgaagaaaaa atttttttag ctgttttgtt gttttttttc at
//
LOCUS       DAOTAX010000059        10786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_5216_length_10786_cov_32.275743, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000059 DAOTAX010000000
VERSION     DAOTAX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10786
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5216_length_10786_cov_32.275743"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..684
                     /locus_tag="PLM04_06770"
     CDS             79..684
                     /locus_tag="PLM04_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HON02812.1"
                     /translation="MKRRILILFTVLFTVFGQSLFAQMDEPIPPGGARLMVEIKGNDT
                     IFLAYLHDLWVFPKMNFKNKKEEQYYWKMVRDVKRTLPYAKLVAQELSETNKKLMMMP
                     NDKERKKFLAQYEKDIYQKYEPELKKLTLSQGKLLIKLIDRECNNTSYDLIKLYRGSF
                     RAFFYQSFARLFGANLKSEFDYNEKDKILDRIIVLVEAGQL"
     gene            696..1259
                     /locus_tag="PLM04_06775"
     CDS             696..1259
                     /locus_tag="PLM04_06775"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HON02813.1"
                     /translation="MTIFDNTKEQKKEIRNRIRELMRQISDTQKEKQAESVFLKIEIL
                     PAFVQAKNILLYWSLPDELPTHTVVEKWATDKQILLPSVKGENLVIKKYSGLHALKKG
                     AFGIEEPLEIFDWHSPIDLVIVPGVAFDKKKNRLGRGKGYYDRFLKSIDALKIGVGFD
                     CQLIEKIPVGKFDVPMNKVVVPSLIID"
     gene            complement(1267..1992)
                     /locus_tag="PLM04_06780"
     CDS             complement(1267..1992)
                     /locus_tag="PLM04_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02814.1"
                     /translation="MPYRRLPNTDQSRLRALRIALEKLENQDFTHQIVNFKTIHEAES
                     ILGVFENQMQQYRQALKNQVEANKRYQKLVGNVRMYISHFIQVLNLSVIRGDIKKEHK
                     KLYQLDPEVNNVPDLTSEAALLHWGKCIIDGENERLRLGGFPIYNPTIAKVKVHYEIF
                     KENKLNQKLYQSNTSRTLEDLGNLRIKVDEIIIDIWNQVEANFKDEKPYSKIQKCKEF
                     GLIYYYRKGEKKLLPEDAEEQKK"
     gene            complement(2044..3012)
                     /locus_tag="PLM04_06785"
     CDS             complement(2044..3012)
                     /locus_tag="PLM04_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HON02815.1"
                     /translation="MIPFSEITKIINTELNKLNWDKKPEGLYRPIAYVLSLGGKRIRP
                     SLVLMACNLFSDKVESAIAPALGIEIFHNFTLLHDDIMDKAELRRGQPTVHKKWDENT
                     AILSGDLMQIAAYQLIGKVDELHLKKVLDIFSETAVEVCEGQQYDMEFEHREDVSPEE
                     YLEMIRLKTAVLLGAALKIGAIVGNANSKDANLLYEFGINLGMAFQLKDDLLDVYGDE
                     QFGKKIGGDIVANKKTYLLISAQKLAKGADAKELEKWLKTDSENETKVEAITALYNRL
                     EVRDICENKMQEFFEKSIDCLNRISVEAAKKIELRHLAEQLMSRKE"
     gene            complement(3020..4015)
                     /locus_tag="PLM04_06790"
     CDS             complement(3020..4015)
                     /locus_tag="PLM04_06790"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HON02816.1"
                     /translation="MKKNIAIVAGGDSSEVVVSLKSAEGLHSFMDKQRFNVWIVTIIG
                     KTWQVECSDNEKYPIDRNDFSFTRKGEKIVFDFAYITIHGTPGENGILQGYFNLIGLP
                     FSCCDVLAAAITFNKFTCNQFLKNFDVKTAASVLLRKNEEIDLHEISAKTGFPCFVKP
                     NAGGSSFGVSKVKSIDQLEPAIRKAFSEDDEVVVESFLAGTEVTCGMYKTKAKRVVFP
                     LTEVVSQNEFFDFAAKYNGQVQEITPARISGSLTEKVQCQTARIYDILGCKGLVRMDY
                     IISEKEEIFLLEVNTTPGMTATSFIPQQIKAAGLNITDVLTEIIENELNYPTLIK"
     gene            complement(4107..>4472)
                     /locus_tag="PLM04_06795"
     CDS             complement(4107..>4472)
                     /locus_tag="PLM04_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HON02817.1"
                     /translation="HYKVLERLTYVTLVECRLETGRTHQIRVHMKHIGHPLFNDERYG
                     GNEILRGTTSAKYRQFIKNCFDICPRQALHAKTLGFVHPTTGKQMNFDSELPEDMQQL
                     LTKWRNYNTGSAAVEKEDF"
     assembly_gap    4473..4572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4573..5036)
                     /locus_tag="PLM04_06800"
     CDS             complement(<4573..5036)
                     /locus_tag="PLM04_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HON02818.1"
                     /translation="MEVTKWEDFSDSAEEPELFEHFRFIADKGQSPIRIDKYLVNCMA
                     NVSRNRIQEAADAGNILVNDKPVKANYKVKPNDVISIVMTYPPSQFEIIPQNIPIDIV
                     YEDDDLMVINKQPGLVVHPGVGNYEGTLLNALAYHFKDNPKFDFNDSRIGLV"
     gene            complement(5066..5905)
                     /locus_tag="PLM04_06805"
     CDS             complement(5066..5905)
                     /locus_tag="PLM04_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HON02819.1"
                     /translation="MDFKKFWKESFAAFVVKNLLLAIAIFLALTWITLFIIDIYTHHG
                     EAEIVPDMKGLYVEEAENILREKGLYPKIIDSVYVRDKKAGIIIEQVPPPKSTLKKNR
                     PVYLIINSTQVQKVPVPEVIDVSSRQAIAMLQSVGLDISKIEYKPSEFKDLVIDIKYN
                     GVSILPGTKIPESSSVVLVVGSGYGEGMFSVPSLKGMGLQEAKQAALSNSFVIGMVNF
                     DIPPKGNDADYFIYRQLPKAGTSMPAGSRIDIWLTSSPAKLLEDKEEDQIIILPENDE
                     VFF"
     gene            complement(6082..6240)
                     /gene="rpmH"
                     /locus_tag="PLM04_06810"
     CDS             complement(6082..6240)
                     /gene="rpmH"
                     /locus_tag="PLM04_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HON02820.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMTTANGRRVLASRRAKGRKKLTV
                     SDEGRPKL"
     gene            6673..8220
                     /locus_tag="PLM04_06815"
     CDS             6673..8220
                     /locus_tag="PLM04_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HON02821.1"
                     /translation="MKKRTIWILIILMVVTIVGLVLVQIRYVRINAEMIENQFKENVQ
                     RSLFQAVRLVEENEALQYLSETLEDVDYSAKSKGLIKSGNAQKDVLGMTTDSMAMDSG
                     KDFTLNQKLPSIQLSTKHGKATIEETSRYLQKKFQQDFSRSKTILDQAVFRWLRESDQ
                     KEISQRVNFDELNDILQTLLENNGVTLPFNYSVVDKQGRLVYKHTNISVDEQKISQTG
                     NVFTQKLFPFEETNQPYYLQVDFPTRQNYILNSMNLLLPSVVLVILILTIFIIAIIII
                     FRQKQLNNIKNDFVNNMTHELKTPIATISLASQMLQDPNIGKTPEMLQHVSKVIHDET
                     QRLSFQVEKVLQMAIFEKEKSPLNLTEIHINSLIQDVIHSFSLRVTNSGGKIIPSLKA
                     EKDLALIDEVHFTNVIFNLMDNALKYSNKPLLLNIETWNEKDRIFISIEDNGIGIRKE
                     DLKRIFEKFYRVSTGNLHNVKGFGLGLAYVKKIITDHKGTIKVESEPNIGTKFTISLP
                     ILKNQEK"
     gene            8217..8927
                     /locus_tag="PLM04_06820"
     CDS             8217..8927
                     /locus_tag="PLM04_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HON02822.1"
                     /translation="MTDEKIKILLCEDDENLGMILREYLQSKGYDVDLLPDGEAGYKA
                     FTHKKYDLCILDVMMPKKDGFTLAADIRALNSEVPIVFLTAKSMKEDILQGFKLGADD
                     YLSKPFSMEELLYRIESIMRRIRGEKAREVTVYQIGNYTFDSQKQLITINGETKKLTT
                     KESELLSLLAANMNNVLERNYALKTIWVDDNYFNARSMDVYITKLRKLLKDDPNVAII
                     NIHGKGYKLIAPAVKEKN"
     gene            9159..10601
                     /locus_tag="PLM04_06825"
     CDS             9159..10601
                     /locus_tag="PLM04_06825"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HON02823.1"
                     /translation="MKLRNFILTVLLFSFLLTYAEIPAGYYNSADGKNASALRTALKT
                     IITNGHSVTSYAGLWTAYKTTDVNANGKIWDMYSNCSFTYGTDQCGSYSNECDCYNRE
                     HTTPQSWFNEAPPMVSDLFNVYPTDGKVNGMRSNYPYGEVGSATYTSGNGSKLGASSF
                     SGYSGTVFEPVDEYKGDFARTYFYMATRYADECGTWTGEATAVYSSSNGLTEYGKNLF
                     LKWSREDPVSQKEIDRNNAVYGIQNNRNPFIDYPGLEEYIWGTKTSEVFYVNASVSKP
                     SVSSPTATNVTYNSAVLGGTVTSTGGGTITETGVYYSTTDGFADGTGTKVSTSSPSTS
                     TFTVSINGLNSSTTYYFKAFATNSAGTGYSSQSSFKTSTLSSGNDPEIILGNPVATGS
                     TLNFGKVTANSTKSLLIKTKNLTGDLSVSVTGEMFNVSTSTISKSNAEEGYLLNVNYQ
                     PTATGSHTGTLTISGGGLTSNYVVQLTGSK"
BASE COUNT         3447 a         1995 c         1914 g         3330 t
ORIGIN      
        1 attttaagag gaaattcaat atttgttttt tgtttttttg aaacaatatc tgaaaaaata
       61 tgcgtaactt tgtttgctat gaaacgccgg attttgatat tgtttactgt tttatttacg
      121 gtttttggtc aatcgttatt tgcacaaatg gatgagccca ttccccccgg tggggcacgt
      181 ttaatggtag aaattaaggg aaatgatacc atttttctgg cttatttaca tgatctttgg
      241 gtatttccca aaatgaattt taaaaacaaa aaagaggaac agtattattg gaaaatggtt
      301 cgtgatgtga aaagaacgct gccttatgcc aaattagtgg ctcaggaatt atccgaaacc
      361 aataaaaagt tgatgatgat gcccaacgat aaggaaagaa aaaaattttt ggctcaatat
      421 gaaaaggata tttatcagaa atacgaaccg gaattgaaga agttaacatt gagtcaggga
      481 aagctattaa ttaagcttat cgatagagag tgcaacaaca cttcctatga tttgataaaa
      541 ttgtacagag gcagtttcag agcttttttc tatcaaagct ttgcaagatt atttggtgca
      601 aatttaaaat cggagttcga ttataacgaa aaagataaaa ttctggatag aattatcgtt
      661 ctggtggaag ccgggcaact ataaatgacc attaaatgac catttttgac aatacaaagg
      721 aacaaaaaaa ggaaattcgg aatcggattc gggaattaat gcggcaaata agcgacacgc
      781 aaaaagaaaa acaagccgag tcggtttttt tgaaaatcga aatactccct gcctttgtac
      841 aggcaaaaaa catactttta tattggtcgc tgcccgatga acttccaact cataccgttg
      901 tagaaaaatg ggctacagac aagcaaatct tacttccttc agtaaaagga gaaaatctcg
      961 tgataaaaaa atattccggc cttcacgcac tgaaaaaagg tgctttcggc atcgaagaac
     1021 cattggaaat ttttgattgg cattctccca tcgatttggt tattgttccc ggtgtggctt
     1081 tcgacaaaaa aaaaaataga ttgggaagag gaaaagggta ttacgaccgt tttttgaaaa
     1141 gtatagatgc gctaaaaata ggtgtcggtt tcgattgtca attaatagaa aaaattccgg
     1201 tcggaaaatt tgacgtgcca atgaataaag ttgttgtacc ttctttgatt atcgactaag
     1261 atacgtttat tttttttgtt cttccgcatc ttctggcaaa agtttttttt ctcccttgcg
     1321 ataatagtaa atcagtccaa attccttgca tttttgaatt tttgaataag gcttctcgtc
     1381 cttgaaattg gcctccacct gattccagat gtctataata atttcgtcca cttttattct
     1441 taaatttccc aaatcttcga gtgtccgcga agtattgctc tgataaagtt tctgattgag
     1501 cttgttttcc ttgaaaattt cgtaatgaac cttaactttg gcaatggtag gattataaat
     1561 gggaaaaccg cccaagcgca acctctcatt ttctccatca attatacatt taccccaatg
     1621 cagcaaagcg gcttcgctgg tcaaatccgg aacattgttt acttcaggat caagctgata
     1681 aagtttttta tgttcctttt taatatcgcc acgaattaca gaaagattta aaacctgaat
     1741 aaaatgagaa atatacattc ttacattgcc aaccagtttt tggtaacgtt tattggcttc
     1801 aacctgattt tttaatgctt gtcggtattg ttgcatttga ttttcaaaaa cgcccaaaat
     1861 gctttccgcc tcatgaattg ttttaaaatt gacaatctga tgcgtaaaat cctgattttc
     1921 gagcttttct aatgcaattc gtaaagcccg aagtcgtgat tggtccgtat ttggtaatct
     1981 tctgtatggc atgttttttt caaaaaaaaa ttcttgaaat ttatcgatgt caaagttaaa
     2041 aaattattcc ttgcgagaca ttaactgctc tgccaaatgc cttaattcga ttttctttgc
     2101 tgcttcaaca gaaatcctgt tcaaacaatc aatggatttt tcaaaaaatt cctgcatttt
     2161 attttcacaa atgtctctta cttcgagtcg gttgtaaaga gcggtgatag cttctacttt
     2221 tgtctcattt tcagaatcgg ttttcaacca cttttccaac tctttggcat ctgctccttt
     2281 tgccagtttt tgggcagaaa tcaacaaata ggtttttttg ttggctacaa tatctccacc
     2341 aatttttttc ccaaattgct catcgccata aacatcgagc aagtcatcct tcagctgaaa
     2401 agccattccc aagttgattc caaattcata aagcaaattg gcatccttac tgtttgcatt
     2461 gccaacaata gccccaattt tcaatgcagc tccaagtaat acagctgttt taagacgaat
     2521 catttccaga tattcttcag gtgatacatc ttcacgatgt tcaaattcca tgtcgtattg
     2581 ttgcccttca caaacttcga cagctgtttc ggaaaatata tccagcacct tttttaaatg
     2641 caattcatca actttcccaa tcaattggta agcagctatt tgcattaaat ctccggaaag
     2701 aattgcggta ttttcatccc attttttgtg aaccgtcggc tgcccgcgtc tcagttccgc
     2761 tttgtccata atgtcgtcgt gtaacagcgt gaaattatga aaaatttcaa ttcccaaagc
     2821 cggagcaata gcactttcca ccttgtcgga aaaaaggttg caggccatca gaaccagcga
     2881 aggacgaatt cgcttgcctc caagtgaaag cacataggca atgggacgat aaagtccttc
     2941 aggttttttg tcccaattca acttattcag ttctgtatta ataatttttg ttatttcact
     3001 gaaaggaatc attataaatt tatttaatta aagtagggta attcaattca ttttcaataa
     3061 tttctgtcag cacatccgta atattcaaac cggcggcttt tatctgttgc ggaataaagc
     3121 tggtggcagt cattccgggg gtggtgttaa cttccagaag gaaaatctcc tccttttcgg
     3181 aaataatgta atccattctg acaagtcctt tacaacctaa aatatcgtaa attctggcag
     3241 tctggcactg aactttttcc gttagtgagc cactgatgcg tgccggcgta atttcctgaa
     3301 cctgaccatt atatttggcc gcaaaatcaa agaattcatt ctggcttacc acttcggtta
     3361 acgggaaaac gactcgtttt gcttttgttt tatacattcc gcaagttacc tccgtaccgg
     3421 caaggaaact ttctaccacc acttcatcgt cttcagaaaa agctttacga atagccggtt
     3481 ccaattggtc aatacttttc accttgctaa cgccaaaact gctgcctcca gcattgggct
     3541 taacaaaaca aggaaatcct gtttttgcag atatttcgtg caggtcaatt tcttcatttt
     3601 ttcgcaacaa aacagaagct gccgttttaa catcaaaatt tttaagaaac tgattgcagg
     3661 taaatttgtt gaaagttatg gcagccgcta acacatcgca acaggaaaaa ggcaacccaa
     3721 tcagattgaa ataaccctgc aaaatgccat tttcacccgg tgttccgtgt atggtaatat
     3781 aggcaaagtc aaatacaatt ttttctccct ttcgagtgaa actgaagtcg ttccggtcaa
     3841 tcggatattt ttcgttatca gagcactcca cttgccaagt tttgccaata atcgtaacaa
     3901 tccatacgtt gaatcgctgt ttatccataa atgaatgcaa tccttcagcg ctttttaacg
     3961 ataccactac ttctgaagaa tctccacctg caacaattgc tatatttttt ttcatccaaa
     4021 aaaataaaaa attaagtatt tatttgtctt ctttactgaa aaatcacagt tacatttgcc
     4081 taatgaaaaa taaactttgg aaataactaa aaatcttctt tctccactgc tgccgacccg
     4141 gtattataat ttctccattt tgtgagcaac tgctgcatat cttcaggcag ttcgctatcg
     4201 aaattcattt gttttccggt agtaggatga acaaatccca atgttttggc atgtaatgcc
     4261 tgacggggac aaatatcgaa gcaattttta ataaactgcc tatatttggc agaagttgta
     4321 cctcttaaaa tttcatttcc accataacgc tcgtcattaa acaaaggatg accaatgtgc
     4381 ttcatgtgaa cacgaatctg atgcgtacgc cctgtttcga gtcggcattc taccaatgtt
     4441 acataagtca ggcgttccaa cactttataa tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nntgaaccag cccgattcgc gaatcgttga aatcgaactt tggattatcc
     4621 ttaaaatgat aagccagcgc attcagcaaa gtcccctcgt aattgccaac acccggatga
     4681 accactaaac ccggctgctt gttgataacc ataaggtcat catcttcata aacaatgtca
     4741 atgggaatgt tttgcggaat aatttcaaac tgacttggag gataagtcat tacaatggaa
     4801 ataacatcat tgggtttcac cttgtaattg gctttcaccg gcttgtcgtt gaccagaata
     4861 tttcctgcat cagcagcttc ctgaatccga ttacgtgaaa cattggccat gcaattgacc
     4921 aaatatttgt caatccgaat ggggctttgc cctttatccg ctataaatcg aaaatgttca
     4981 aaaagttccg gctcttcagc cgaatcggaa aaatcttccc attttgttac ttccaacaat
     5041 ttctttactc gattttattg gttttttaaa aaaatacttc gtcgttttcc ggcaaaatta
     5101 ttatttgatc ttcttctttg tcttccaata atttagcagg agaggaagtt aaccaaatat
     5161 ctattcgact gccggcaggc atagaagtcc ccgctttagg aagctgccga tatataaaat
     5221 agtctgcatc atttcctttg gggggaatat caaagtttac catccctata acaaaagaat
     5281 tggaaagagc ggcttgtttg gcttcctgca gtcccattcc ttttagcgaa ggaacagaaa
     5341 acataccctc gccataacca cttccaacaa ccaaaactac tgaactgctt tcagggattt
     5401 ttgtcccggg taaaatactg actccgttat attttatgtc tatgaccaaa tctttgaact
     5461 ctgatggctt atattcaatt ttacttatgt caagtcctac agattgcagc atggcaatag
     5521 cttgacgaga agaaacatct ataacttcag gaactggaac tttttgaact tgagttgaat
     5581 tgatgatcag atataccggt ctatttttct taagtgttga tttaggaggt ggaacttgtt
     5641 caataataat tccggctttt ttatctctaa catacacgga atcaataatt tttggataca
     5701 atcctttttc tcttaaaata ttttctgctt cttcgacgta caaacctttc atatcaggga
     5761 caatttcagc ttcaccatga tgtgtataaa tatcaattat gaacaatgtt atccatgtca
     5821 aagctaaaaa aattgcaatg gccagtaaaa ggtttttaac tacaaatgct gcaaaagatt
     5881 ctttccaaaa ttttttgaaa tccataagtt atgttaaagt cttttgaaca aagttgaaga
     5941 aataaacttc tgtgaaaaaa tttattccaa aagtacagaa ataatttata ttatcggtgt
     6001 ataacaaaca aaaaaagtaa aaacctctta cgaagttctt actttgaata acgttagatg
     6061 taattttttt ggtgtatctg attacaattt tggacgtcct tcatcggaaa ctgtcagttt
     6121 ttttcttcct tttgctctgc gtgaagccaa aaccctgcga ccattggcag tagtcattct
     6181 ttcacgaaat ccgtgtttgt ttcttctttt tctttgcgaa ggttgaaatg tccttttcat
     6241 cgtgtttggt atttgatgtt gtttttactc cagattttta gaccacaaaa gtaatgtttt
     6301 tttttgaaat gccaaacata gtttagaaaa tagatgctat aaaacagaaa actctttacg
     6361 ataaattttt taaagaaaat tgagtttaca gctattgcat attgcagttt aaaatcgttg
     6421 ttgtattttt aacgaactaa taactacttg ccggctttga gaaacgaata tcatttaaaa
     6481 tttacttaat cattaaaaaa agaattattt ttttaaacta aaaatggaat agtctcgtta
     6541 tgtactaaac atttttcgtt agaaattgtt tttattaaga tatgaattgt ttatgttaat
     6601 ttaatattgt taaatcttat aaaaaactat tttaattact tcattttaag taaatttgca
     6661 aaagtatagt gtatgaaaaa gagaacgatc tggatattga tcattttaat ggtagttaca
     6721 attgtgggtt tggtactcgt acaaatccgc tatgtacgaa taaatgccga aatgatcgaa
     6781 aaccagttca aagaaaacgt tcagcgaagt ttatttcaag ctgtgcgact ggttgaagaa
     6841 aacgaagcat tgcagtactt gtcggaaaca ttggaagatg tggattattc agccaaaagc
     6901 aagggattaa taaaaagtgg aaatgcacaa aaagatgtgc tgggaatgac aaccgacagc
     6961 atggcgatgg attcaggtaa agactttaca ttaaaccaaa aattgccgtc gatacaatta
     7021 tcgacgaaac atggtaaagc aacaattgaa gaaacatcgc gatatttgca aaaaaaattc
     7081 caacaggatt tttcacgttc aaaaaccatt ttggatcagg ctgttttcag atggctcaga
     7141 gagtcggatc aaaaggaaat ttctcaacgg gtaaactttg acgaactgaa cgacatactg
     7201 caaaccctct tagaaaacaa cggcgtaacg ctgccgttta attattcagt agtggacaaa
     7261 cagggaagat tggtttacaa gcataccaat atctcagtag atgagcaaaa aatatcgcaa
     7321 accggaaatg tatttaccca aaaacttttc ccgtttgagg aaaccaacca accttactac
     7381 ctgcaagtag attttcctac caggcaaaat tacattttga attcgatgaa tttattactg
     7441 ccatcggttg ttttggtaat acttattctg acaattttta tcattgcgat tatcattatt
     7501 ttcaggcaaa aacaattaaa caacatcaag aatgattttg tgaacaacat gacacatgaa
     7561 ctgaaaactc ccatcgctac tatttcgttg gcttcgcaga tgctgcagga tccaaacatt
     7621 ggaaaaacgc ccgaaatgct ccaacatgta tcaaaagtaa tacatgatga aacacaacga
     7681 ttaagttttc aggtagaaaa agtactgcaa atggccattt ttgaaaaaga aaaatcgcct
     7741 ttaaatttga ccgaaataca tattaattct ttgattcagg atgttataca cagtttttcg
     7801 ttgagagtaa ccaacagtgg tggaaaaatt attccttcgt tgaaagccga aaaagatttg
     7861 gcactgattg atgaagttca tttcacaaac gtgattttca acttaatgga caatgcgctg
     7921 aaatatagca acaagcctct gctcctaaat atcgaaacat ggaacgaaaa agacagaata
     7981 ttcattagca ttgaagacaa cggaataggt attcgaaaag aagacttaaa gcggattttt
     8041 gaaaagtttt atcgggtttc caccggaaac ctgcacaatg tgaaaggatt tggacttgga
     8101 ttggcttatg taaaaaaaat cataaccgat cataaaggga caattaaagt ggaaagcgaa
     8161 cccaatattg gaactaaatt tactatttca ttaccaattt taaaaaatca agaaaaatga
     8221 cagacgaaaa aattaaaatt ttattgtgcg aagatgacga aaatcttggc atgattttaa
     8281 gagagtatct gcaatcgaaa ggatacgatg tagatttgtt gcctgacggc gaagccggct
     8341 ataaagcctt tacgcataaa aaatacgatt tgtgcatttt agacgtcatg atgccaaaga
     8401 aagacggatt tacactggca gccgacattc gtgcgctgaa tagcgaagtt cccattgtct
     8461 ttctgacagc caaatcgatg aaagaagata ttttgcaggg tttcaaactg ggggcagacg
     8521 actatttgtc gaaacctttc agtatggaag agttgctgta tcgaatcgaa tcgatcatgc
     8581 gccgtatcag aggcgaaaag gccagagaag ttaccgttta tcagattggc aattacacat
     8641 tcgactctca aaagcaactg attaccatta atggtgagac caaaaagctg accacgaagg
     8701 aatcggaact tttgagcctg ctggctgcca atatgaacaa cgtgctggaa cgtaattacg
     8761 cattaaaaac catttgggtg gatgacaatt acttcaatgc cagaagtatg gatgtttata
     8821 tcaccaaact tcgcaaattg ttgaaagacg atcccaacgt ggccatcatt aacattcatg
     8881 gcaaaggtta caagttgatt gctcctgccg taaaagaaaa gaattaaatc attaactttc
     8941 agatttttaa gaggctgttc cgttttttca gagcagcctt ttttattttt acccgaaaaa
     9001 atttttaagg aaaagtatca acattcaaaa atttggctta aattagcacc cttttctaaa
     9061 aaaaaaatca aatgaaacat gctgaataaa ggcttgttga tttttgatga atagaaataa
     9121 cgaaatagct tcaatattat gaattaaaaa atcgacttat gaaattgcgt aattttatcc
     9181 tgaccgttct attattttca tttttgctca cttatgccga aattcctgcc ggttattaca
     9241 acagtgctga cggtaaaaat gcttctgcat tgcgaacggc tcttaaaaca atcattacca
     9301 acggacattc tgtaacgagt tatgccggat tgtggacagc atacaaaact accgatgtga
     9361 atgcaaacgg taaaatttgg gacatgtatt ccaattgctc atttacttat ggtaccgatc
     9421 aatgtggcag ttacagcaac gaatgcgact gctacaacag agaacatacc acaccacaaa
     9481 gttggtttaa cgaagcccct ccgatggttt ccgatttgtt taatgtttat ccgactgatg
     9541 gcaaagtgaa cggcatgcga agcaattatc cttacggaga agtaggttca gcaacgtaca
     9601 cgtcgggcaa cggaagcaaa ttaggtgcaa gttcgttttc cggttacagc gggactgttt
     9661 ttgaacctgt agacgaatac aaaggcgatt ttgcacgaac ctacttttac atggcaacgc
     9721 gctatgccga tgaatgcgga acatggacag gtgaagctac tgccgtttat agttcttcta
     9781 atggattgac ggaatatgga aaaaatcttt tcctaaaatg gagtcgtgaa gatccggtga
     9841 gtcagaaaga aatagaccga aacaatgctg tgtatggaat tcaaaacaac agaaatccgt
     9901 ttatcgatta tcccggttta gaggaatata tttggggaac aaaaacttcg gaagtttttt
     9961 atgtcaatgc ttctgtttcc aagccttctg tgtcttcacc aaccgccacg aatgtaacct
    10021 ataacagtgc tgttttgggc ggaacagtta caagtactgg aggcggaacc attacggaaa
    10081 caggagtata ttacagcact accgatggtt ttgccgatgg aacaggtacc aaagtatcta
    10141 cttcttctcc ttcaacgagt acgtttacag tttccataaa cggattaaac tcttctacaa
    10201 catattattt caaagctttt gcaaccaaca gtgcaggaac gggttattct tctcaatcaa
    10261 gttttaaaac ttcgacctta agttcgggaa acgacccaga aattattctg ggaaatccgg
    10321 ttgcaactgg atcgacatta aatttcggaa aagttacggc aaattccacc aaatcattgt
    10381 tgataaagac caaaaatttg accggagatc tttctgtcag cgttacggga gaaatgttca
    10441 acgtttctac cagcactatc tccaagtcaa atgctgaaga aggttatctg ctcaatgtca
    10501 attatcagcc gacagcaacc ggctctcata cgggtacttt aactatttca ggtggaggat
    10561 taacttccaa ttatgttgtt cagctgacag gttcaaaata ataggatacg gtttcccgca
    10621 aataaagcaa atagcgttgt cggattcaat ggcaacgctt taagtcatga caaaaattta
    10681 aaaatagaat cccctcttaa aatctgattt tttcaaaaaa gagaaaaaat aagatttttt
    10741 tgaagagaag tattaaattt tttgttgttt cgatatttta caataa
//
LOCUS       DAOTAX010000060        10410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_5446_length_10410_cov_31.584259, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000060 DAOTAX010000000
VERSION     DAOTAX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5446_length_10410_cov_31.584259"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1212
                     /locus_tag="PLM04_06830"
     CDS             7..1212
                     /locus_tag="PLM04_06830"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HON02824.1"
                     /translation="MKYLIILGDGMADEPIESLGNRTPLQVANKPNIDKIAELGRSGM
                     LDTIPEGFSPGSEIANLSVMGYDVAKVFEGRGSLEAASMGVPIEKGEMAMRCNLICIE
                     NGKIKNHSAGHISNEEAAELIKFLNEKLGNEIISFYHGVSYRHLLKMKGGNKNLKCTP
                     PHDVPGTPFKEVMIQPEVPEAEATARLLNELTLQSQSLLENHPVNLKRAAEGKDKANS
                     IWLWSPGYKPEMKTLMELYNLKSGSVISAVDLIKGIGVYAGLKVINVEGATGLYNTNY
                     EGKAQAAINALRTDEFVYLHIEASDEAGHEGDVDLKIKTIEYLDRRVVKPIFDEISSW
                     KEPVTIAILPDHPTPCAIRTHTKSPVPFIIYRPGEQPDKVTVYDEFSAREGSYGLLKG
                     PEFMKNLIK"
     gene            1338..2672
                     /gene="thrC"
                     /locus_tag="PLM04_06835"
     CDS             1338..2672
                     /gene="thrC"
                     /locus_tag="PLM04_06835"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HON02825.1"
                     /translation="MKYYSTNKGISGVSLKDAVIKGLADDKGLFMPEQIKQLPPSFFD
                     TIINMSFQEIALAVAEAFFGEDVDNEALKNIVYETLSFDVPLVHVKDNIFSLELFHGP
                     TLAFKDVGARFMSRLLRYFIQKEGEKNVNVLVATSGDTGSAVANGFLGVEGIHVFVLY
                     PKGLVSPIQECQFTTLGKNITALEIDGTFDDCQRLVKNAFADKELNSSLKLTSANSIN
                     VARFLPQAFYYFYAYAQLKAIHPKFTQEELEMVICVPSGNFGNITAGLFAKEMGLPVK
                     RFIAANNRNDIFLQYLKTGKYQPRPSVQTIANAMDVGDPSNFARILDLYHGSHDKISS
                     LISGVSYTDEEISATIKECYRETGYLLDPHGACGYRALRENLTNNENGIFLETAHPAK
                     FLDTVEKIIGEKVEIPAKLQEFMKGEKQSIPLKNDFESFKNFLLSAALGNKD"
     gene            complement(2682..3128)
                     /locus_tag="PLM04_06840"
     CDS             complement(2682..3128)
                     /locus_tag="PLM04_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HON02826.1"
                     /translation="MLEFTELGYLGLFIGSFLAATVVPFSSEVIFSTMVFGGLNAWTC
                     VFVATAGNWLGGMTSFYLGKLGKIEWIEKYFHVKKEKIDQFLEKFHKYGEWFVFFSFL
                     PFIGDAIAIAAGFLRARWWATAAFMLAGKFCRYVVWMYVNGLILMI"
     gene            complement(3135..4130)
                     /locus_tag="PLM04_06845"
     CDS             complement(3135..4130)
                     /locus_tag="PLM04_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02827.1"
                     /translation="MGRRQISIKSLITPTIPGTVLLVLFCVGWWYFPYLIGKQPFMLH
                     DDPPFVLSVFSFNPAINNFLSLLLTVLNAILIQNLNLRFNILRKRSLLPFLIFLFLMA
                     GWYPLHFIWELHLALSIFILAFYYFFDMYRQPAAVEQAFMGSFLIAVASLFLPPLLWM
                     VPVVWINFLQMRAFTLRTFLASILGILSPWILYAGGYFVFQDNFDFNKLIFSRFFDGG
                     KYDFSSFQSIDLIYFVSLAAILLISIVYIYMNFQQDSISTRSKLNMIIIQMLFLLGIS
                     IFHASVFYCFLPFAVFFYVLLLSHPFSTRENNFFSILFLVFCLLNVAFVLFKIIA"
     gene            complement(4164..6260)
                     /locus_tag="PLM04_06850"
     CDS             complement(4164..6260)
                     /locus_tag="PLM04_06850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HON02828.1"
                     /translation="MKKLIVAVMIGTLLTGCSTNKDNPFLQAYDTPFSVPPFDKIKTE
                     HYLPAFREGIKTHQKEIDAIAENSEAPTFANTVEALDFSGELLKKVSSVFFNLYSTDT
                     NDKMEAIANEVTPLLTEHSDNLYLNKALFQRIKTLYDKRSTLGLNAEQNRLLEKYYKN
                     FVRNGAALNDEQKEKLREINKQLSLAELQFGQNVLAETNAYQKWITDEKELVGLPENV
                     KQSAAEAAKEAGREGQWLFTTKKASFIPVLQYSENRELRRELLTAYNKRADNGNKNDN
                     KPVINKIVKLRVQKAQLLGYPTWADYELDDTMAKTPQKVYEFLASVWEPSVRKAREEA
                     AELQKMMDAEGKGEKLEPWDWWFYAEKLRKAKYDLEEEALKPYFKLENVRKGVFDLAS
                     KLYGLKFKKLENIPVYHPDVEVFEVTDANDSLIGILYTDYYPRAGKQAGAWMNNISEQ
                     YIKDNINHRPVICNVGNLTKPTSDKPALLSMDDVETLFHEFGHALHGLLSQCTYPSLS
                     GTNVARDFVELPSQIMENWCWEPEVIKTYAFHYKTGEVMPNELIEKIQQASTFNQGFA
                     MTELLSAALLDMDYHTIKDTADIDVTAFENATMKRIGMIPQIVVRYRSTYFNHVFSGG
                     YSAGYYSYTWAEVLDADAFQAFKETGDIYNSEIAASFRKNILERGDSDDPMKLYVAFR
                     GKEPQPDALLIKRGLK"
     gene            complement(6405..7331)
                     /locus_tag="PLM04_06855"
     CDS             complement(6405..7331)
                     /locus_tag="PLM04_06855"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HON02829.1"
                     /translation="MQVIEANSGIKIEPCVATVGFFDGVHIGHRFLMEELKKIASTQH
                     LKSVVVTFDQHPRKVLNADFQPKLLTTLDERLFQIGSTGVDACVVLNFTREMSLLSAR
                     DFLKEVLHDQLNVQTLLVGHDHRFGHNREDGLSEYIRYGEEFGIKVIEAQRFATEDMP
                     HISSSVIRRALLDGDVETANRLLMYEYSFEGKVVNGFKVGHKIGFPTANLIPLSEEKL
                     IPGVGVYAVRARCNEKKFKAMMDIGYRPTFGDDNKLTIEVHIIDFDEDIYQKNIRIDF
                     IRRLRDEIKFDTVEELIEQLYRDREEVLAMNF"
     gene            complement(7400..8041)
                     /locus_tag="PLM04_06860"
     CDS             complement(7400..8041)
                     /locus_tag="PLM04_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HON02830.1"
                     /translation="MIHFKNISALIFDFGGVLINLSRQQCIDSFRSLGVKEVEKYLDD
                     FNQSGFFLQLEQGKISPEQFRNEVRKLSDHTLTDEQIDRAFCSFLLDIPEEKLKLLLE
                     LRKRFKIYLLSNTNLIHMTFCRAQHFHYNGYSMDDFFDKCYFSYELGITKPDIRVFNS
                     VIEDIGLPPQQCLFLDDGLQNIEQAQKTGLQTYWVKQQEDLSFLLQPDVFVFD"
     gene            8136..8213
                     /locus_tag="PLM04_06865"
     tRNA            8136..8213
                     /locus_tag="PLM04_06865"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8171..8173,aa:Ile,seq:cat)
     gene            complement(8553..8798)
                     /locus_tag="PLM04_06870"
     CDS             complement(8553..8798)
                     /locus_tag="PLM04_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02831.1"
                     /translation="MGADQGHYGGYMQEHFNALLDTIANIKGKFLLSSYPNPELENYR
                     KQFGWQSNDVNMSLSASRKSGRRKLECLTFNYHLSNI"
     gene            complement(8813..10171)
                     /locus_tag="PLM04_06875"
     CDS             complement(8813..10171)
                     /locus_tag="PLM04_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02832.1"
                     /translation="MNQTTDKVTITYPITAKKADGQTVTFNLIQTITKSLKGEPGEDG
                     ENGTNGLDGNFIEYRYAKNGSTSTAPSIVVTDLAPSGWEITPPSTGLLEYLWMTKAKK
                     NYSGTQLLESWSTPVRIKGDIGDKGDVGASPVYQGVYSSHKTYYGTSQRVDIVQYNSN
                     YYVARVDAGTFSNIVPTDTSKWNLFGAQFESIATNLLLAEGANIADWIIADGKITAQA
                     TTDDDTPRAKLDGVNGKINFASDIDKYTEDGDSESVKQEISIDSLNGKIEVRTSDYDV
                     SYMNSQGIFTNRAGIQALPASSGIELKAAVVALGFGDLKKDAYYNKGAICGVYGSSLN
                     NNSDPAPSYGGYFDKLRANGLYLNCRQINSSTTLTQNDVIISLYNTSTITITLPSSTY
                     TGQIILLRINNNSSINISGNGNSIMRVDGAIVTNVTVGTRGELIFLIWDGQYWLYSTT
                     EQ"
BASE COUNT         3308 a         2122 c         1859 g         3121 t
ORIGIN      
        1 attctgatga aatatctcat tatattggga gatggaatgg ccgacgagcc cattgaatct
       61 ttaggaaaca gaactccttt gcaagttgcc aataaaccga acatagacaa aattgccgag
      121 ttgggaagaa gtggaatgct ggatactatt cccgaaggat tttctcccgg cagcgaaatt
      181 gccaatttaa gtgttatggg ttacgacgtt gcaaaagttt ttgagggacg tggttcgttg
      241 gaagcagcca gtatgggtgt ccccattgaa aagggcgaaa tggccatgcg ttgcaatttg
      301 atttgtatag aaaacggaaa aatcaaaaat cactctgccg gacacatcag taatgaagaa
      361 gctgccgaat taattaaatt tttaaacgaa aaacttggca acgaaataat cagtttttat
      421 catggcgttt cttatcgtca tttgttaaaa atgaaaggag gaaataaaaa cttaaaatgc
      481 acgcctccgc atgatgtacc cggcacaccg ttcaaagagg tgatgataca acctgaggta
      541 ccggaagccg aagcaacggc tcgtctgtta aatgaattga ctcttcaatc gcagagttta
      601 ctggaaaatc atccggttaa cctgaaaaga gcagcagaag gcaaggacaa ggccaacagc
      661 atttggctgt ggtcgcccgg ttacaagccc gaaatgaaaa cattaatgga actttacaat
      721 ctaaaaagcg gatcggtcat ctcggcagtc gatttaataa agggaattgg ggtgtatgca
      781 gggttgaaag tgatcaatgt cgaaggagct acgggtttgt acaataccaa ctatgaagga
      841 aaggctcaag cggccatcaa tgccttgcga accgatgaat ttgtatattt gcatattgaa
      901 gcaagcgacg aagcaggaca tgaaggggat gtagatttga aaatcaaaac gattgaatac
      961 cttgatcgaa gagttgttaa gcctattttt gacgaaattt cctcgtggaa agaaccggtt
     1021 accatcgcta ttcttcctga tcatccaact ccgtgcgcca ttcgtacaca tacaaaaagt
     1081 cccgttcctt tcattattta tcgccccggt gaacaacccg acaaagttac ggtttatgac
     1141 gaattttctg cccgggaggg aagttatggc ctcttgaaag gtccggaatt tatgaaaaat
     1201 ctgatcaaat agttttaatc aaataactat cgaaaatttc ttatgaaatc cacatgtatt
     1261 acatacacaa acacgaatga atataatatt cagacatgtg ggttccatca gaccattaaa
     1321 aaataaatta tattctgatg aaatactaca gcaccaacaa aggaatttcg ggtgttagtc
     1381 tgaaagatgc cgttatcaaa ggcttggccg acgacaaagg acttttcatg ccggagcaaa
     1441 tcaagcagtt gcctccttct tttttcgata ccatcatcaa tatgtccttc caagaaatag
     1501 cattggcagt ggcagaagca ttttttggag aagatgtaga taacgaagca ctgaaaaata
     1561 ttgtgtacga aaccttgtcg tttgatgttc cgttggtgca tgttaaagac aatatcttca
     1621 gtctggaact ttttcatggg ccaacgctgg cgtttaaaga cgttggtgcc agattcatgt
     1681 cccgtttgct ccgctatttt atccagaaag aaggcgaaaa gaacgtaaat gtattggttg
     1741 ccacttccgg cgacactgga agcgctgttg ccaacggttt tttaggcgta gaaggcatac
     1801 acgtttttgt cctttacccg aaaggattgg tcagtcccat tcaggaatgt cagtttacca
     1861 ctttaggaaa aaatattacc gcgctggaaa tagatggcac attcgacgac tgtcaacgtc
     1921 tggttaaaaa tgcttttgca gataaggaat tgaattcttc cctaaaactt acttcggcaa
     1981 actctattaa tgttgcccga ttccttcctc aggcttttta ttatttttat gcttatgcac
     2041 aattaaaagc cattcatcca aagttcacac aggaagaatt ggaaatggtt atttgcgtac
     2101 caagtggaaa ttttgggaat attactgccg gcctttttgc caaagagatg ggattgcctg
     2161 tgaaacgctt tattgctgcc aacaaccgca acgatatttt tcttcaatat ctgaaaaccg
     2221 gtaaatacca accacgtcct tccgtacaaa ccattgcaaa cgctatggat gtaggcgacc
     2281 caagcaattt tgcccgtatt ctcgatttat atcatggttc gcatgataaa atcagttctt
     2341 tgatttcggg tgtcagctat accgacgaag aaatttctgc caccattaag gaatgttatc
     2401 gtgaaaccgg ttatttgctt gatcctcatg gagcttgtgg ctatagagct ttgcgcgaaa
     2461 acttgacaaa taacgaaaac ggaatttttc tggaaacagc tcatccagct aaatttttgg
     2521 atacggtaga aaaaataatt ggagaaaaag ttgaaattcc tgccaaactt caagaattta
     2581 tgaaaggcga aaagcaaagc attcctttaa aaaacgattt cgaatcgttc aaaaactttc
     2641 ttttatcggc tgctttgggg aataaggatt gacaacattt gctaaatcat taatatcaaa
     2701 ccgttcacat acatccaaac gacgtaacgg caaaatttcc cggcaagcat aaaagctgcc
     2761 gttgcccacc agcgggcacg taaaaaaccg gctgcaatag ctatggcatc gccaatgaaa
     2821 ggcaggaaag agaaaaacac aaaccattct ccatatttgt gaaatttttc cagaaattgg
     2881 tcaatttttt cttttttgac atggaaatat ttttctatcc attctatttt ccccaatttc
     2941 cccaaataaa aagatgtcat tcctccaagc cagttgccag ctgtagccac aaaaacacaa
     3001 gtccaagcat tcaaaccacc aaaaaccatt gtggaaaata taacttccga actgaaagga
     3061 accaccgttg ctgcaagaaa tgaccctata aaaagtccca aataacccaa ttccgtaaat
     3121 tccaacatga gtggttaggc aattatttta aacaacacaa aggccacatt tagcaagcaa
     3181 aaaacaagga acaaaatgct gaaaaaatta ttttctctcg tagaaaaagg atgagacaaa
     3241 agcaatacat agaaaaaaac cgcgaaggga aggaaacagt aaaagaccga agcatgaaaa
     3301 atggaaattc ccaacaaaaa caacatttga ataatgatca tattcaactt cgaccgcgtg
     3361 ctaatgctat cctgctgaaa attcatgtag atataaacta tcgaaatcaa taaaattgcg
     3421 gccagtgaaa caaaataaat caaatcaatg gattgaaatg aagaaaaatc atattttcct
     3481 ccatcaaaaa aacgactgaa aatcaatttg ttgaagtcaa aattatcctg aaaaacaaaa
     3541 tacccacctg cataaagtat ccacggactc aatatcccca aaatggaagc aagaaaagtt
     3601 ctcagcgtga aagctcgcat ttgtaaaaaa ttaatccaaa ccaccggcac catccagagc
     3661 aaaggtggca aaaacagact tgcaacagct atcagaaagc tacccatgaa agcctgctca
     3721 acagcagccg gctgacgata catgtcgaag aaataataaa aagccagaat aaaaatgctc
     3781 aatgccaaat gaagctccca aataaaatgc aacggatacc atccggccat taaaaacaaa
     3841 aaaatcaaaa aaggcaataa agaccttttt ctgagaatat tgaagcgtag attcaaattc
     3901 tgaatcaata tcgcattcaa tacagttaaa agcaaactta aaaaattgtt aatagccgga
     3961 ttgaatgaaa aaacagaaag aacaaacgga gggtcatcgt gaagcataaa tggctgcttc
     4021 ccaatcaaat aggggaaata ccaccagcct acacagaaca gtacaagcag cactgttccg
     4081 ggtatagtag gagttattaa agatttaatg gatatttgac gcctgcccac tgaaagtttt
     4141 tttaaaaatt ttcctcttca acattatttt aatcctcttt ttatcaaaag agcatcgggt
     4201 tgaggttcct taccgcgaaa agccacatac aacttcatgg gatcatccga atcgccccgc
     4261 tcaagaatat tcttacgaaa agaagcagct atttcggaat tatatatatc gccagtttcc
     4321 ttaaaagcct gaaaagcgtc ggcatcgagt acttcagccc atgtatagct atagtatccg
     4381 gcagaatatc ctcccgaaaa aacatggttg aaataggtac tccgataacg caccactatt
     4441 tgaggaatca tgccgatgcg cttcatggtg gcattttcaa aagcggtaac atctatatcg
     4501 gcggtatctt taatggtatg ataatccata tcgagcaatg ctgccgaaag cagttccgtc
     4561 atggcaaacc cttgattgaa agtacttgcc tgctgaattt tctctatcag ttcattgggc
     4621 atcacttctc cggttttata atgaaaagca taagttttta ttacttccgg ttcccaacac
     4681 cagttttcca taatttgtga aggaagttct acaaaatcac gagctacgtt tgtacccgag
     4741 agagaaggat aagtgcactg agaaagtaaa ccgtgcaaag catgtccaaa ctcatgaaat
     4801 aaagtctcca cgtcgtccat gctcaacaaa gcaggtttgt cggaagtggg cttggtcaga
     4861 ttgccaacat tacaaataac gggtcgatga ttgatattat ccttgatgta ttgttccgaa
     4921 atgttgttca tccatgcgcc tgcctgcttg ccggcacgag gatagtaatc ggtatagaga
     4981 atgccgatca gcgaatcgtt ggcatcggtt acttcaaaaa cttcgacatc gggatggtaa
     5041 accggtatat tttcgagttt tttaaacttc aatccgtaaa gtttggaagc taaatcaaaa
     5101 accccttttc tgacattctc gagtttaaaa tatggtttta atgcttcttc ttccaaatcg
     5161 tatttggctt ttcgaagttt ttcggcataa aaccaccaat cccacggttc caatttttct
     5221 ccttttcctt cggcatccat cattttttgc aattctgccg cttcttctcg tgctttacgt
     5281 acggaaggtt cccacacgga agccaaaaat tcataaactt tttgaggtgt tttggccatt
     5341 gtatcgtcca attcataatc ggcccaagtg ggataaccca ataattgggc tttttgcacg
     5401 cggagtttca caattttatt gatcaccggt ttgttgtcgt ttttattgcc attatcggca
     5461 cgcttgttat aagcggtcaa taattctcga cgcaattcac gattttcgct atattgaaga
     5521 acgggaatga aactcgcctt ttttgtggtg aaaagccatt gtccttctct cccagcctct
     5581 ttggcagcct cagctgccga ctgtttgaca ttttccggca atccgaccaa ttctttttcg
     5641 tcagtaatcc atttttgata agcatttgtc tctgccaata cgttttggcc aaactgcaac
     5701 tctgccaaac taagctgttt gttgatttct ctcaattttt ccttttgctc atcgtttaat
     5761 gcagcaccat tgcgaacaaa atttttgtaa tacttctcca gcaacctgtt ttgttcggca
     5821 ttgagtccaa gtgtggaacg tttatcgtaa agggttttaa ttcgttgaaa cagcgcctta
     5881 ttgagataaa gattatcact gtgttccgtc agaagcggcg ttacctcgtt ggcaatggct
     5941 tccattttat cgttggtatc ggtagaatag agattgaaaa atacggaaga aacttttttc
     6001 agcaattctc cactgaaatc caatgcttca accgtattgg caaaagttgg cgcttcgcta
     6061 ttctcggcaa tggcgtcaat ttccttctga tgagttttta ttccttcacg aaaggcagga
     6121 agataatgtt cagtcttgat tttatcaaat ggaggaacgc taaaaggagt atcataagcc
     6181 tgcaagaaag gattgtcctt gttggtagaa caaccggtta ataatgtacc aatcataacg
     6241 gcaacaatta attttttcat aaataaatga ttttaacaaa tttgtcgaaa aatattttcg
     6301 gttaaataag atttttgatt atcaattgct atgttttata cagcaaaacg aaaaattgtt
     6361 tgctttcctt tcaaaaatac aacgaaagca tgaaaaatga aatttcagaa attcattgcc
     6421 aaaacttcct ccctgtcccg atacaactgc tcaatcagtt cttctaccgt atcgaattta
     6481 atttcatctc tcagccgacg gataaagtca attctaatgt ttttttgata aatatcttca
     6541 tcgaaatcga taatgtgaac ctcaatcgtc aacttgttgt catccccgaa tgtaggtcga
     6601 taacctatgt ccatcatggc tttgaatttt ttttcgttac aacgcgctct aacggcatac
     6661 actccaacac cgggaattaa tttttcttca cttaacggaa tcaggtttgc agtggggaat
     6721 ccaattttat gacctacctt aaagccattt acaacctttc cttcaaaaga atattcatac
     6781 atcaacaatc gatttgccgt ttccacatct ccatcgagta gtgctcgacg aataacggaa
     6841 gaactgatat gaggcatatc ttctgtagca aaccgctgag cttctataac ttttattcca
     6901 aattcttctc cgtagcggat atactccgac aatccatctt cccgattatg accaaaacga
     6961 tggtcgtgcc ccaccagcag agtctgtaca ttgagttgat cgtgaagaac ctcttttaaa
     7021 aaatcccgcg cggacaacaa agacatttca cgcgtaaaat tcagcaccac acaagcatca
     7081 acacctgtcg aaccgatttg gaaaagtctt tcgtccaacg tagtgagcaa ttttggctga
     7141 aaatcggcat ttaaaacttt acgcggatgc tggtcgaaag ttacaaccac gcttttcaaa
     7201 tgttgcgtcg aagctatctt tttcaattct tccattaaaa atcgatgacc aatatgcacg
     7261 ccatcaaaaa aacctactgt ggcaacacaa ggttctattt ttattccgct gttcgcttct
     7321 atcacttgca tgaaattccc actaaaatga aatacattga aaatttattt ggaaaaacct
     7381 attgattttt ctacttgttt taatcaaaaa caaaaacatc gggttgaagc aggaaactca
     7441 aatcttcctg ttgtttcacc caataagttt gcaagccggt tttttgtgcc tgttcaatat
     7501 tttgcaaccc atcatccagg aaaaggcatt gctgcggtgg tagcccaata tcctctatca
     7561 cggaattaaa cacacggatg tcgggcttgg ttattcccaa ctcataagaa aaataacatt
     7621 tgtcgaaaaa atcatccatc gaataaccgt tataatgaaa atgctgtgct ctgcaaaaag
     7681 tcatatgaat aagattggtg ttgctcaaca aataaatttt aaaccttttc cgaagttcca
     7741 acaacaactt taatttttct tccggtatat ccagcaaaaa tgaacaaaaa gctcggtcta
     7801 tctgctcatc ggtcaaagtg tggtcagata attttctgac ttcgttgcgg aattgctccg
     7861 gtgaaatttt tccctgttcc aattgaagaa aaaaaccgga ctgattgaaa tcgtccagat
     7921 atttttccac ttcttttact cccagagacc gaaaactatc aatacactgt tgacggctca
     7981 ggttgatcaa caccccgcca aaatcgaata tcagagccga tatattttta aaatgtatca
     8041 tttaaaagaa aaataacaaa gaattttttc aaacacacaa aaaattatta tttttgcatt
     8101 cgctttgcaa agataagcaa ttattcgcaa atgcagggcc catagctcag ttggttagta
     8161 gcacctgact cataatcagg gggtcgctgg ttcaagccca gctgggccca ccatcagaaa
     8221 agctttctta ttcattaaga tagctttttt tttgaacaat tattattttt caaggctggc
     8281 cggctgcttt ctcttctgaa caatacttct gcaatatttt ccagcaaaat cttcaggcat
     8341 ttttttatat gtacaaataa tttcggtcaa tcagaatccc ttaattttca gttttaaatt
     8401 tacttgataa tcaataatat gagacgaatt ttattatgaa aatattttga ttataatcga
     8461 aaaaacaaaa tgtacaaaag tataaaacaa attgtacaaa aaaacaaaaa ataagcaggc
     8521 gtttaaatta tacctgctta tttaactatt atttaaatat tgcttaaatg ataattaaac
     8581 gtcagacatt cgagttttct tcgacctgat tttctggagg cggaaaggga catgtttaca
     8641 tcgtttgatt gccaaccgaa ttgcttacga tagttttcca attcaggatt cggatagctg
     8701 cttaatagaa acttgccttt gatatttgcg attgtatcga gcaaggcatt gaaatgctcc
     8761 tgcatgtagc ctccataatg accctgatcg gctcccattt ctcctttata aattattgtt
     8821 ctgttgttga atataaccaa tattgtccat cccaaataag aaatattagc tcaccacgtg
     8881 tgccaactgt tacatttgta acaattgctc catcaactct cattatacta tttccattac
     8941 cggatatatt tatacttgaa ttgttattaa ttctcaataa gataatttgt ccagtatatg
     9001 tacttgaagg taaagtaatc gtaatcgttg aagtgttata aagtgatatt ataacatcgt
     9061 tttgtgtcaa tgttgtcgat gaatttattt gccgacaatt taaataaaga ccattggctc
     9121 gtaatttatc gaaatatcca ccataagaag gagcaggatc agaattattg tttaatgatg
     9181 acccataaac tccacaaatt gctcctttgt tataatatgc gtcttttttt aaatcaccaa
     9241 aacctaatgc tacaacagcc gcttttaact ctattccaga tgaagcaggc aaagcttgta
     9301 tcccagctct attagtaaat atcccctgag aattcatata tgatacatca taatctgatg
     9361 tccttacttc tatttttcca ttcaggctat cgatgcttat ttcttgtttc accgattctg
     9421 aatccccatc ttctgtatat ttatcgatat cggatgcgaa atttatttta ccatttactc
     9481 catccaattt tgctcttgga gtatcgtcat cagtagtcgc ttgtgctgtt atctttccat
     9541 ctgctataat ccaatcagcg atatttgccc cttctgccaa caataaattc gttgcgatac
     9601 tttcaaattg tgctccgaat aaattccatt ttgaagtatc tgttggcact atattactaa
     9661 atgttccggc atcgactcgt gcgacatagt aatttgagtt atattgcact atatcaactc
     9721 gctgtgaagt accgtaataa gttttatggc ttgaataaac tccttgataa actggtgaag
     9781 cacctacatc ccctttatct ccaatatcac ccttaatccg tacaggcgtt gaccaacttt
     9841 caagtaattg tgtacctgaa taattctttt tagctttcgt catccagaga tattcaagaa
     9901 gcccagttga cggaggtgtt atttcccatc cggaaggagc taaatctgtt actacaattg
     9961 aaggtgctgt tgaggtgcta ccattttttg cataacgata ttctataaaa ttaccatcca
    10021 aaccattagt tccattctcg ccatcttctc caggctcgcc ttttaaagat tttgtaattg
    10081 tttgaataag gttgaatgta acagtttgtc catctgcttt ttttgctgta atcggataag
    10141 tgatagttac tttatctgtc gtctggttca ttgcagaatg gtttgcaacg ttacaactgt
    10201 tagtcccaga gcctgaaaga gcaccaaccg ttatttttcc cgtaggagaa acaacaggcg
    10261 tcccaacagt aaatttaccg tttgcaagtg ttgtattata ttgtaatgct gttgctcctt
    10321 caaatacttg tattttacat cctgaattat tataattatg attatccgca aactttccta
    10381 aattaaaaag gataaattat ggtatatttt
//
LOCUS       DAOTAX010000061         9921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_5809_length_9921_cov_24.615852, whole genome shotgun sequence.
ACCESSION   DAOTAX010000061 DAOTAX010000000
VERSION     DAOTAX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5809_length_9921_cov_24.615852"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(488..1408)
                     /locus_tag="PLM04_06880"
     CDS             complement(488..1408)
                     /locus_tag="PLM04_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02833.1"
                     /translation="MKIQILFLNHIRLFIIFLMLILTQNYGLTQNQVPIVVSTPPNSI
                     WGPPNQYCFFAYEIKVEDDDSDVSFVAKKIPSWLQLNKYYPKSAFLQGTPEYVNSDSV
                     IIEISDGVNIIQHRFELTVRPLYNPPSFGTAPMTEAFVDSLYTYEAIVNPGAVDNSII
                     SLECINKPEWITFEKDYQIGNTDERKRARLTGKPLNEGEYEVYLTAKYGITNVSEVNQ
                     IFTISVKNSNTAINSIDADKLKLYLSFDRNILHFSKCLSTILVFDVFGKLIKSYKNTS
                     IIDVTNYTNGIYLLKTKDFDGKIHLNKFLK"
     gene            complement(1816..2694)
                     /locus_tag="PLM04_06885"
     CDS             complement(1816..2694)
                     /locus_tag="PLM04_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02834.1"
                     /translation="MRDSINKIQILPMSEDGFEDGKKDLNTIQFDYLLRDLPIIEQGR
                     YYYEKSGISEPKDTMILFKFKTKIIGSGILLGKDNDKRGKYLQFDSNSISIYVKPIDI
                     EEMTYVWPDFKEYGQSKKMLDINLLDKFVTRFSDRLIKNVSDEELFQMQVEMSTPTES
                     EIIDKPIPKTEKYSSTSKYFVRDSNVSKGALIKAHYTCELNPEHKYFVSRFTGKNYVE
                     AHHLIPMEFQDKFETSIDIEANVVSLCPVCHKMIHFAVLESKTDLILNLLSKRIDRLG
                     HAGITISNDELIEMYK"
     gene            complement(3639..3824)
                     /locus_tag="PLM04_06890"
     CDS             complement(3639..3824)
                     /locus_tag="PLM04_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02835.1"
                     /translation="MQKVITVTTRAYIDGKEKFTETEYPELKKSLEEGYYVKQIIPVI
                     QNNGNGVYYDTIFVLEK"
     gene            complement(4045..6003)
                     /locus_tag="PLM04_06895"
     CDS             complement(4045..6003)
                     /locus_tag="PLM04_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02836.1"
                     /translation="MHQTDLNKVVFYSKEDMVGPYNLQKGEYILRADTKTNYTDINEV
                     LELYNLKKYIDNNLYLKNWTQDDIDSFKIKVIEYGKVVGQFMATINDSNILSIYESTL
                     KNYTHSFWELVNNQGIFKSISKTNFSKLLTNEPHLIHEILIHKGIVEYYDKEIKNFLM
                     TYPQSAEILLSIYEIKDEFSKKQKYLPKSLTVEEKEIILSNYLDSPEVNYNYIGLIQN
                     ARNRSDFKVSDKTRLKAKRLHKSETEKFFAGNKGMRYGVSVSFPENVSKIKDVMIDDD
                     FIVNCTYSLDFIKQNNTPYLLFLNFIDLFEYLDHQHRINLVSKKNKMGVFERFMGVHS
                     ENEYRVGIKFNLMEKTSQLQLVGYIKILSDMGLSLENILHQVFTSSFQEKYEFAENAL
                     FSIPSALSYFEKVRLLAPEFESVLKQFKLFVENGSIDFELLQISSNPTAIKDIPSLNK
                     NKYIYLNENNKELVSCVNLFFSDQTLLAYVEPFEEKNYHTFFDLLTNEQVKFSSYEEH
                     QKPELNYLIEQGFISIDSNDFVQITNPARIIILKDLFDNEVASFYRYPFEFRQEAEKM
                     ETEKMIYFESSLFSKPEQAYFNFFLNKSEFTNGLDLRNSYLHGTQAKPDEIQKHEYAY
                     FTYLKLLILTFLKIDDDLMIYKALKDSI"
     gene            complement(6137..6946)
                     /gene="arsM"
                     /locus_tag="PLM04_06900"
     CDS             complement(6137..6946)
                     /gene="arsM"
                     /locus_tag="PLM04_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HON02837.1"
                     /translation="MEKNIKSVVKDTYTVIAQQSKEQNETSCCGVGGCCSTVDYAVFS
                     ENYRQLKGYHEEADLGLGCGIPTQFASIKEGDSVLDLGSGAGNDCFVARAIVGETGKV
                     VGIDFTDAMLEKARKNNAKLGFQNVEFVKGDIEDMPLEDNQFDVVISNCVLNLVPDKR
                     KAFAEIKRVLKPGGHFCVSDVVVKGNLPEKLKNDAEMYAGCVSGAIEMQEYLDIIRET
                     GFQSVTVHKQKEVQLPQEILQKYLTEKEIRQFNQKEIGIFSITVAADKAIE"
     gene            complement(6958..7314)
                     /locus_tag="PLM04_06905"
     CDS             complement(6958..7314)
                     /locus_tag="PLM04_06905"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HON02838.1"
                     /translation="MNVIYTIVKVLLSSVVLVAVSEISKRSTFVGSLLASIPLVSLLA
                     IVWLYIDTKDTAQIASLSTGIFWLVIPSLVFFIVLPLLLKRSLNFWLSLIISSVLTII
                     AYFIMLYLLKKSGIKL"
     gene            complement(7311..7730)
                     /locus_tag="PLM04_06910"
     CDS             complement(7311..7730)
                     /locus_tag="PLM04_06910"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HON02839.1"
                     /translation="MKILILCTGNSCRSQMAEAFLRSFDNRLTVCSAGTEPAEKVNEK
                     AIQVMREAGIDISRNRTKSVSQYLQETWDYVITVCADADRNCPVFSGKVKHRLHIGFD
                     DPAKVTGSEEFVLNEFRRIRDAIKEKFHQFYQENLQL"
     gene            complement(7741..8082)
                     /locus_tag="PLM04_06915"
                     /pseudo
     CDS             complement(7741..8082)
                     /locus_tag="PLM04_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            8513..9835
                     /gene="mtaB"
                     /locus_tag="PLM04_06920"
     CDS             8513..9835
                     /gene="mtaB"
                     /locus_tag="PLM04_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HON02840.1"
                     /translation="MIDTNIYKTKKAAYYTLGCKLNFAETSQLGKQLSAAGYRKVHAG
                     EEADVCIVNTCSVTDTADHKCRQAIKRLKRQHPNAIMVVTGCYAQLNPEEIAAIDGVN
                     LVLGAGEKFDVVNFIEQWNAENSVEIHKTPIQKVKEFHPSCSGTDRTRYFLKVQDGCD
                     YYCTYCTIPFARGRSRNASIRETVELATKAVAEGAKEIVLSGVNIGDFGKSTGENFFD
                     LVKALDALPEEVRFRISSIEPDLLTDELIEFIALSHHFMPHFHIPLQAGSNEALLLMK
                     RRYSTELFAHKVEVIKKWLPDAFIGVDVIVGTRGETEELFEKSKNFIASLDISQLHVF
                     TYSEREGTKMIQMEQNVPLAERKRRSDELHLLSEQKTQLFYESQIGKDAEVLWEGRHN
                     GSKMVGFTANYIRVERPYEKKKVNTIEKIKLGGWNDQHSALIALGYHD"
BASE COUNT         3047 a         1746 c         1731 g         3397 t
ORIGIN      
        1 aatatgattt attttttaaa ggtctgatgg aacccacatg tctgaatatt atatttattc
       61 gtgtttgtgt atgtaataca tgtgggtttc atattcttta aattcaatgt ttataatggt
      121 cgctgtcact ttttaaaatt gtgcataacg gtttatttac gcaataaata tacaacattt
      181 gtaaataaaa attataaatc acgtaaatta ttttcgagtt tatatcaaac ctctttcaat
      241 cgcatgatta ggaatgactt ccccacacga tacaattgtg cgggagcgag gggcaattca
      301 gttttacgta aaaaataagc tctcgtttta taaaagactt tgaacacgat acaatcacat
      361 gggagaaggg tgtggattac aaatcctctg attaacattg cttcgaattg caaatcctcg
      421 cttcgatggt ttaatagatt gactaataat tgtaagcaaa attataattc ttgttttcgt
      481 tatttgatta ttttaaaaat ttattcagat gaattttccc atcaaaatct tttgttttta
      541 ataaataaat cccgtttgtg taattggtaa catcaataat tgaagtgttt ttgtacgact
      601 taataagttt tccaaaaaca tcaaatacta atatagtaga taaacattta gaaaaatgaa
      661 gaatatttct atcaaaagat aaatataatt tgagcttatc agcatctatt gaatttattg
      721 ccgtgttact atttttaaca ctaatagtaa atatctggtt aacttcagaa acatttgtta
      781 taccatattt cgctgttaag taaacctcat attcgccctc atttaaaggt ttccccgtta
      841 atctagctct ttttctttca tctgtatttc ctatttgata atctttttca aatgtaatcc
      901 attcaggttt atttatacat tcaagagata ttatgctatt atcaacggca ccaggattta
      961 ctattgcttc ataagtgtat aatgagtcaa caaaagcttc agtcatcgga gctgtaccaa
     1021 aactgggcgg attatataaa gggcgaactg ttaattcaaa tctgtgttgg ataatattta
     1081 caccatcaga tatttcaatt atcactgaat ctgagtttac atattcgggg gtgccctgta
     1141 aaaatgctga tttcggataa tatttattta actgcagcca tgatgggatt ttttttgcaa
     1201 caaaacttac atcagaatca tcatcttcga ctttaatttc gtaagcaaaa aaacaatatt
     1261 gattaggtgg tccccaaatc gagtttggtg gggtcgatac aactattggc acttgatttt
     1321 gggttaatcc ataattttga gttaaaatga gcattaaaaa aattatgaac aaacgaatat
     1381 gatttaaaaa taaaatttga attttcatca gaatatgatt tattttttaa aggtctgatg
     1441 gaacccacat gtctgaatat tatattcatt cgtgtttgtg tatgtaatac atgtgggttt
     1501 catattcttt aaattcaatg tttataatgg tcgctgtcac tttttaaaat tgtgcataac
     1561 agtttattta cgcaataaat ataccacatt tgtaaataaa aaacaataaa ttatgaaaat
     1621 tattttagag tttatacaaa atcttttttg attgaatgat taaggatatc atttaatttt
     1681 tcgtagaaat aaccttctgt tttatgaaag atttttcaca caatataatc gcgaggaagc
     1741 aacgtgtgga ttaaaaatcc tctgattaac attgcttcgg attgcaaaat ctttgcttcg
     1801 aggtttttta ttctattatt tgtacatctc aattaattca tcattactaa tcgtaattcc
     1861 agcatgtcca agtctatcaa ttcgcttaga taacaaattc aaaattaaat cagttttgct
     1921 ttctaaaaca gcaaagtgaa tcattttgtg acaaactgga cacaatgaaa ccacatttgc
     1981 ctcaatgtct atacttgttt caaacttatc ttgaaactcc attggaataa ggtgatgagc
     2041 ttcaacgtaa tttttccccg taaatctgga aacaaaatat ttatgttcag gatttaactc
     2101 acaagtataa tgtgctttta ttaatgctcc ttttgaaaca tttgaatccc ttacaaaata
     2161 tttggatgtc gaactatatt tttcagtttt tggtattggt ttatcaatta tttcagattc
     2221 agtcggtgta ctcatttcta cttgcatctg aaataattct tcgtcagaaa cgtttttaat
     2281 taatctatcc gaaaagcgtg taacaaattt atccaatagg tttatatcca acattttttt
     2341 tgattgacca tattctttaa aatctggcca tacgtaagtc atttcctcaa tatcaattgg
     2401 cttaacatat attgatatag aattagaatc aaattggaga tatttgcctc ttttgtcatt
     2461 atcttttcca agtagaatcc cagacccaat aatcttggtt ttaaatttga ataaaatcat
     2521 tgtatctttt ggttcagaga taccagattt ttcataataa taacgtcctt gctcaattat
     2581 tggcaaatct cgcagtagat aatcaaattg aattgtattt aaatcttttt tgccatcctc
     2641 gaatccatcc tcagacattg gaagaatttg aattttattg atagaatctc tcatgtgcga
     2701 tttttttagg cttgttgcta acgtcttatt tccgccataa atatacaaca tttgtaaata
     2761 aaaaacataa attatgaaaa ttattttata gtttatacaa aatctttttt gattgaatga
     2821 ttaaggatat catttaattt ttcgtagaaa taactttctg ttttatgaaa aacatttcac
     2881 acaatataat tgtgcgggag cagggtgtgg attaaaaatc ctctgattaa cattgcttcg
     2941 gattgcaaaa tctttacttc gaggggaaac tactatgact tgaaatttgt tttgcctttc
     3001 aggcagagtg attgggatgg ttttcagttc cgcagaataa attctgcggt tatgaaaatt
     3061 aggcttttca agccggtttt attttgaaaa taattgattg attcaaactg ttcctattag
     3121 gttttttgcc tgaaaggcaa aagtttcata accgaaggtc gaagacctgc ggatggaaga
     3181 gaaaagacaa tccctgcctg caaggcagaa cttttcagtg tgaagtttac aaatccttgc
     3241 ttcgagggtg tagattacaa atcctctgat ttacattgct tcgaattgca aatccttgct
     3301 tcgagggttc atacctgcga gatttgtttc aacgttacaa acgttatttt gaatcgcata
     3361 aaaatcaagc tccgaaggag ctaaattttg gtagaacaat aaaacctcaa acatggaagc
     3421 cctgtagggg cgaaattatc acattagctg ccgcacgatt tcatcgtgtg gggaaggcta
     3481 tgagatacaa ccgattttgt tttaatggat ttacccattt tcgaaaaaga gcagtaacag
     3541 aatggggatt ccaaatccac tcaataacat tgcttcggat gcaaaatctt tgcttcgagg
     3601 ggtttttaag atgtcagtag aagtaacctt aacgtagtct atttctcaag aacgaaaata
     3661 gtgtcataat atacaccgtt cccattattt tgaattactg ggataatttg ttttacataa
     3721 tatccttctt caagtgattt tttcaattca ggatactcag tttcggtgaa tttttctttt
     3781 ccgtcaatgt aagctcttgt tgttactgta attacttttt gcattttctt taatgtttaa
     3841 taagtttata ttcgggttat ttatgagact attttcgctc tttatttttt tttagcactt
     3901 accgctaacg gtttgcggct tgacgtagtg gcgtatttag aagtacttgc tttcaattta
     3961 gcactaatgt ttattagaaa tccgaatgtt caatttagta ttgaatccgc cattttccca
     4021 aactgctgtt agcggttcgg gcttttatat actgtctttc aaagctttat aaatcatcaa
     4081 gtcgtcatca atttttaaaa atgtcaatat tagtagtttt aaatacgtaa aataagcgta
     4141 ctcgtgcttt tgtatttcgt caggcttcgc ttgtgtcccg tgtaagtaac tatttcttaa
     4201 gtcgagacca tttgtaaact cacttttatt caagaaaaag ttgaaataag cttgctcggg
     4261 ctttgaaaat aaagaacttt caaaataaat cattttttca gtttccattt tttcagcctc
     4321 ttgtctgaat tcaaaaggat atcgataaaa tgaagcaact tcattatcaa ataggtcttt
     4381 tagaattatt attctcgctg gatttgtgat ttgaacaaaa tcattgctgt caatggaaat
     4441 aaagccctgt tcaataagat aattcaactc aggtttttga tgttcctcat aactactaaa
     4501 ttttacttgt tcattcgtaa gcaaatcaaa aaaagtgtga taattttttt cttcgaatgg
     4561 ttcaacataa gctaataagg tttggtcgga aaagaataaa tttacacaac ttactaattc
     4621 tttattgttc tcgtttaagt atatatactt gtttttgttt aagctaggaa tgtcttttat
     4681 tgccgttgga ttagatgaga tttgtaaaag ctcaaagtca atgctaccat tttcaacaaa
     4741 gagtttaaac tgttttaata cagactcaaa ttccggggct aataacctaa ctttctcaaa
     4801 gtacgataaa gctgatggaa ttgaaaaaag agcattttct gcaaactcat atttttcttg
     4861 gaaagacgaa gtaaaaactt gatgtaaaat attttctaag gacaaaccca tatcgcttag
     4921 tattttaata tatcctacta attgtaattg agaagttttt tccattaaat tgaatttgat
     4981 acctactcta tactcatttt cggaatgaac acccataaat cgctcaaata cacccatttt
     5041 atttttcttg ctaactagat taattctatg ttgatgatcg agatattcaa acagatcaat
     5101 aaaattcaga aatagtaaat agggtgtatt attttgctta ataaaatcaa gactgtaagt
     5161 acagttgact ataaaatcat cgtcaatcat tacgtcttta atcttactga cattttcggg
     5221 aaagctaact gaaacaccat atctcattcc cttatttcct gcgaagaatt tttcggtttc
     5281 gcttttgtgc aaacgttttg cttttaacct tgtcttgtca gaaaccttaa agtcactcct
     5341 gtttcttgca ttttgaatga ggccaatgta attatagtta acctctggag agtctaaata
     5401 atttgaaaga ataatttctt tttcttcaac tgtcaggctt ttagggaggt atttttgctt
     5461 tttactgaat tcgtctttta tctcataaat tgaaagaagt atttcagcag attggggata
     5521 agtcataagg aagtttttga tttccttgtc gtaatattca actattcctt tgtgaattag
     5581 aatttcgtgt attaaatgag gttcatttgt taatagctta ctgaaattag tttttgaaat
     5641 gcttttaaaa attccttgat tatttactaa ttcccaaaat gaatgagtat aattttttag
     5701 agtgctttca tatatgctca aaatgtttga gtcattaata gttgccataa actgtccaac
     5761 tactttacca tattctatta cttttatttt aaagctgtcg atatcatcct gtgtccaatt
     5821 ttttaaatag aggttgttgt cgatatattt tttcaagttg tacagttcaa gaacttcgtt
     5881 gatgtctgtg taatttgtct ttgtgtcagc ccttaaaata tattcccctt tctgtaggtt
     5941 atatggtcct accatgtctt cttttgagta aaatactact ttgtttaaat ctgtctggtg
     6001 cattctgttg tagtcttttt agggttgcca ctaacgtctt attttcgcta taaatataca
     6061 aattttgtaa aaataaaatt caaaacccac aattatttct tttttaatag agaaaagctt
     6121 gttagcccca caattcttat tcaattgctt tgtctgccgc aacggtgatg ctgaaaattc
     6181 cgatttcctt ttggttaaac tgtcggattt ctttttcggt gaggtatttc tgcagaattt
     6241 cctgcggcag ttgcacttcc ttttgtttgt gaacggtaac cgattgaaat cccgtttccc
     6301 gaatgatatc cagatactcc tgcatctcga tggctcccga tacgcaaccg gcatacattt
     6361 cggcatcgtt tttcaacttt tccggcagat tgcctttgac aaccacatcc gacacgcaaa
     6421 aatgaccacc aggtttcaat acccgtttaa tttcggcaaa cgctttccgc ttgtcgggca
     6481 ccagattcaa tacgcaattg gaaatcacca cgtcaaactg attatcttcc aatggcatat
     6541 cttcgatgtc gcctttgaca aattctacat tttgaaagcc gagtttggca ttatttttac
     6601 gcgctttctc cagcatggca tcggtaaaat caatgccgac caccttaccg gtttctccaa
     6661 cgatggctcg ggcaacaaaa caatcgttgc ctgcaccgct tcccaaatcg agcacgctgt
     6721 cgccctcttt gatggacgca aactgggtag gtatgccgca tcccaatccc aaatcggctt
     6781 cttcgtgata acctttcagt tggcggtaat tttcactgaa tacggcgtaa tcgaccgtag
     6841 aacaacatcc gcccacgccg cagcaggaag tttcgttctg ttctttcgat tgctgagcaa
     6901 tcaccgtgta agtgtcctta acaacggatt tgatattttt ttccatagaa ttgaatgtta
     6961 gagttttatt cctgattttt tgagtaaata gagcatgatg aaataagcga tgatcgtcag
     7021 caccgaagag ataatcagcg acagccaaaa gttgagactc cttttgagca acaggggcag
     7081 cacaataaaa aagacaagcg aaggaatcac gagccagaaa atgccggtgg acaacgaggc
     7141 aatttgtgcc gtgtctttgg tgtcgatgta cagccaaacg atggccagca gcgaaaccag
     7201 cggtatggaa gccaacaggc tgcccacaaa agtgctccgt ttggatattt cggaaacggc
     7261 taccaataca accgacgaaa gcagtacttt aacgatggta taaatgacat tcatagctgt
     7321 aaattttctt gatagaattg gtggaatttt tcttttattg cgtcccttat tcttctgaac
     7381 tcgtttaaga caaattcttc agaacctgta actttggccg gatcatcaaa accaatatgc
     7441 agccggtgtt ttacttttcc cgaaaatacc ggacaattgc ggtcggcatc ggcgcaaacg
     7501 gtaatcacgt aatcccacgt ttcctgcaga tactgactga ccgatttcgt tctgttccgg
     7561 ctaatatcga taccggcttc acgcattacc tgaatggcct tctcgttcac tttctccgcc
     7621 ggttcggtac cggcagagca aaccgtcaaa cgattatcaa acgaacgcag aaatgcttct
     7681 gccatttggc ttcggcaact gttaccggtg caaaggataa gtattttcat atacgtaaaa
     7741 ttaatcttcg ggattgcaac aaatgtcttt gggttgcatg tttgcaaaga attgccgaaa
     7801 agctttactg gctttttccc aattctcctg atcgatgcag tatctcactt tgggcggttc
     7861 gatttcgcct ctgattaatc cggcgttctt caattccgtc aagtgttgcg atacggtagc
     7921 ctttgcaatg ggaagttccc ggcaaatatc gccgaagaaa caactgtcca tcgaagccaa
     7981 gtaccgaaga atgctgatgc gggcaggatg tcccattgct ttggcaaaac gcgccaactc
     8041 ttcgatgtcg aagtcgtagg ttttattttt agggtaagcc atcggtgtat ggatgatttg
     8101 aatttgttgt tcgcaaaatt acgaataata ttcaaaataa aaaatcagtg atattttttt
     8161 gcaagtcaat taatgggaag ttaaaaactg aatgtttgag attgtccatt tttcacatca
     8221 aagtttttgc ccgagaagca acacttgtag cgtttattta acttggccca gactgcaaaa
     8281 gcaacaaatc gaagtttttt acatagtaat gagttgaaat caaaccaaca caatttaatc
     8341 atggccacat cttctttcct ttttgtcgat tattttttat ttatgtatta caaccaactt
     8401 tttattgatt ctcatcagaa aatattttcc ttcagaggat tcaacaaaga tatgataaaa
     8461 agaaattggc tgtttttttg taattttgca gaagaattcg agtaagcaac aaatgattga
     8521 cacaaatata tataaaacca aaaaagcggc ttattataca ttgggttgca aactgaactt
     8581 tgccgaaaca tctcagttgg gcaaacaatt gtcggctgca ggttaccgaa aagtacatgc
     8641 gggcgaggaa gcagatgtat gtattgtcaa tacctgttcg gttaccgata cggccgacca
     8701 caaatgcagg caagccatca aacgactcaa acgtcagcat ccgaatgcca tcatggtggt
     8761 aacagggtgt tacgctcaac tcaatccgga agagattgct gcaattgatg gcgtaaattt
     8821 ggtgctgggt gccggtgaaa aatttgatgt ggtgaatttt attgagcaat ggaatgccga
     8881 aaattcggtt gaaattcata aaacacctat tcaaaaagtg aaagaatttc acccttcctg
     8941 ctccggaacc gatcgtacac gatatttttt aaaagtacag gatggatgcg attattattg
     9001 tacttattgc actattcctt ttgcgcgagg caggagcaga aatgcatcca ttcgggaaac
     9061 ggtggaattg gcaacaaaag ccgttgctga aggtgcaaag gaaattgtgc tttccggtgt
     9121 caacattggt gatttcggaa aatcgacagg agaaaatttc tttgatctgg taaaagcatt
     9181 ggatgcgctt cctgaagaag ttcgtttcag aatttcatcc attgagcccg atttgttgac
     9241 cgatgaattg atagaattta ttgctctcag tcatcatttc atgcctcatt ttcatattcc
     9301 tttgcaggcg ggcagcaatg aagccctgct attgatgaaa agacgatatt cgaccgaact
     9361 ttttgctcac aaagttgaag ttattaaaaa atggttgcct gatgccttta tcggagttga
     9421 cgtaattgta ggaacacgtg gagaaactga agaacttttt gaaaaaagca aaaattttat
     9481 cgcctcgctg gatatctctc aacttcacgt atttacctat tccgaaagag aaggaacaaa
     9541 aatgattcaa atggagcaaa atgttccatt ggcagaaagg aaaaggcgca gtgatgaact
     9601 tcatctcctg tccgaacaaa aaactcagtt attttacgaa agtcagatcg gtaaggacgc
     9661 cgaagtctta tgggaaggcc ggcataatgg ctcaaagatg gtaggtttca cggccaatta
     9721 tattcgggtg gaacgacctt atgaaaaaaa gaaagtcaat accatcgaaa aaatcaagct
     9781 gggcggctgg aatgatcagc attcggcctt aatagcctta ggataccatg attaatttaa
     9841 gcttatcata tcaattccgt ttgcgcaatt atgaaaccca catgtattgc atacacaaac
     9901 acgaatgaat ataatattca g
//
LOCUS       DAOTAX010000062         9557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_6115_length_9557_cov_32.105978, whole genome shotgun sequence.
ACCESSION   DAOTAX010000062 DAOTAX010000000
VERSION     DAOTAX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6115_length_9557_cov_32.105978"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLM04_06925"
     CDS             complement(<1..636)
                     /locus_tag="PLM04_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HON02841.1"
                     /translation="MKVLFIGGTGIISSACTEIAIEKGMEVFHLNRGMTDKDHVPKEV
                     TTIHADIRNGNEAEAKLKGHHFDAVVDFIAFEPQHIEQDIRLFRDRTAQYVFISTASA
                     YQKPPDKLPITEKTPLWNPYWEYSQKKIFCEKLLKSEAKKSGFPFTIVRPSHTYNYKK
                     VPAIGGYTVLHRMLKGQPVVLHGDGTSVWTLTYHRDFAVGLIGLLGNRAAIH"
     gene            908..2503
                     /gene="pckA"
                     /locus_tag="PLM04_06930"
     CDS             908..2503
                     /gene="pckA"
                     /locus_tag="PLM04_06930"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HON02842.1"
                     /translation="MANLDLSKYGITGEYEIVHNPTYEQLFRAETDPKNEGFERGVVT
                     TTGAVAVDTGIFTGRSPKDKYFVKDEITEKAMWWDGVINRPINTEVWDYCRGLVTDRL
                     GSVKKLYVVDCFCGTNPDTRMKVRFVMEVAWQAHFVTNMFIRPSHYELANFGEPDFVV
                     LNGSKVTNPKWKEQGLNSEVFVLFNLSQRMAVIGGTWYGGEMKKGIFSLMNFYLPQKG
                     IASMHCSANVGEDGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGVFNYEGGC
                     YAKVIDLSEENEPDIWRAIRRDALLENVTVLPDGTPDFSKAASKTENTRVSYPIYYIN
                     KIVLPSKAGHASKIIYLSADAYGVLPPVSILDEKSAQYHFLCGYTSKLAGTERGINEP
                     VPSFSPAFGEAFLTLHPTIYAKVLAEKMKKHNAKAYLVNTGWNGTGKRISIKDTRAII
                     DAIIDGSIEKAEKVHIPIMNLTIPKSLNNVNPAILDPRDTYANPAEWEQKAKSLAAQY
                     IKNFEQYLDNENAKELLSAGPQL"
     gene            complement(2591..3586)
                     /gene="trpS"
                     /locus_tag="PLM04_06935"
     CDS             complement(2591..3586)
                     /gene="trpS"
                     /locus_tag="PLM04_06935"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HON02843.1"
                     /translation="METVLSGIRPTGNLHLGNYFGALKNFVQMQNEYNCYFFIADYHS
                     LTTHPTPQNLTENVREILAEYLAAGLDPEKAVIYIQSDVPEVTELYLFLNMNAYVGEL
                     ERCTSFKDKVRQQPHNVNAGLLTYPTLMAADILLHRANKVPVGKDQEQHLEMTRTFAN
                     RFNRMYQVDYFPEPQAFNFGNELIKIPGLNGTGKMGKSEGEGNAIFLSDEPEAIRKKV
                     MKAVTDAGPTQPHQEKSQPIENLFQLMRAVSSAETLAYFEEQYDSCQIKYGEMKKQLA
                     EDMITFTAPFREKIKDLLSNKDYLSNVARMGKEKAQENARKTIRDVREIIGFKSI"
     gene            3791..4606
                     /locus_tag="PLM04_06940"
     CDS             3791..4606
                     /locus_tag="PLM04_06940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON02844.1"
                     /translation="MEKKVILITGASSGIGLSTAIQLMNKGYRVYAASRSGGENSTSK
                     NGGEIISTKMNVNSEEEIKKVLDEILEQNGHLDAVISNAGNGIAGSIEDTSIEEAKYQ
                     FETNFFGAVRVIQACLPIFRKQGYGKIIATSSVAAIVPIPFQAFYSASKAALLIFMQA
                     LDMETKPFGIQCSTILPGDTKTNFTSARKYTAASQSTESVYYEKMKKAVGKMEKDEQN
                     GMPADAIAKAIVKQIVHKSMKAIVVPGFQYQVICWLFNRLPVKFRLKVVSMLY"
     gene            complement(4693..6594)
                     /locus_tag="PLM04_06945"
     CDS             complement(4693..6594)
                     /locus_tag="PLM04_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HON02845.1"
                     /translation="MQKYLDILKQYWGYSSFRPLQDEIIGSIASGNDTLGLMPTGGGK
                     SLTFQVPAMAMDGVCVVVTPLIALMKDQVENLKKRGIAAAAIFSGMSYDEILMTLENA
                     SFGAYKFLYVSPERLATRIFIEKIKQTKVCLIAVDESHCISQWGYDFRPSYLKIAEIR
                     AFLPNAPVLALTATATPEVAEDIQKQLHFPAFNIFKKSFYRSNLAYVVRYTEDKNQYL
                     LKILNSVPGTSVVYVRNRKKTKETAEFLVKNGIVAEHYHAGLNNVKKDEIQTRWKNGK
                     TRVIVSTNAFGMGIDKADVRSVIHLDLPDSLEAYFQEAGRAGRDEKKAFAVLLYNNSD
                     AAKIKKRISDTFPPKEMIRKVYESLGNYYQVGIGSGLDNTFPFDLVDFCTKFHLPVLM
                     AFSSLKILQQAGYIEFTDEEESTSQVLFTVKKEELYQLKQTPKQEKLIHILLRSYTGL
                     FTEPAFIHEEVVAQRLQSTPQEVYQQLVELSKEKVIRYIPRKKTAFLTFTQERVDAKQ
                     IYLSREAYEDRRERFVSRVQSVLNYAQEENVCRSQMLLSYFGEKNSQPCGKCDVCQKK
                     KETEISTAEFEAIRSSIVQVLSNENLPLDIVVQKIHFKEKKVLQVIRFMLDNNQIKEN
                     EKKQLILAK"
     gene            6871..9309
                     /gene="topA"
                     /locus_tag="PLM04_06950"
     CDS             6871..9309
                     /gene="topA"
                     /locus_tag="PLM04_06950"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HON02846.1"
                     /translation="MPKNLVIVESPAKAKTIEKFLGKEYHVVSSYGHIRDLSEKGIGI
                     DFEHNYQPQYKVLPDKKKLVSELKKATEQAELVWLASDEDREGEAIAWHLSEELKLPK
                     EKTRRIVFHEITKDAIVHAIEHPRDLDLNLVDAQQARRVLDRIVGFELSPLLWKKIRP
                     SLSAGRVQSVAVRLIVEREREINQFVPESSYRVTAIFSTTDKDGRSVELKAELQQRFS
                     TKEEALNFLNQCKEAIFRVENITTKPTKRSPAPPFTTSTLQQEAARKLGFSVSQTMLI
                     AQHLYESGKITYMRTDSVNLSDFALKAAEAEITNMLGKKYWKLRHYVTKTKGAQEAHE
                     AIRPTYMNVHEIDGSDQEKRLYDLIWKRTLASQMADAEFEKTTIQIGMQGQPYQFVAS
                     GEVIIFDGFLHLYSESTDDESDSEKSEILPAINVGEKLLPKEILAQERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNRKYIEKGNRPGEKRTYNVLKLVDGKISDRSA
                     TEITGAEKAKLFPTDIGIVVNDFLIEYFPGILSYNFTANVEKEFDNVAAGKVGWTQVI
                     DKFYKKFHPTVEKTLTSTERKVGGRTLGTDPKTGKPVTVKIGRYGPYVQIGTAEDAEN
                     PVFASLRPGQSIDSITLEEALDLFKLPREVGEFEGKPLTVAIGRFGPYIRQGNSFYSI
                     PKGEDPLNVSLERAIEIIQHKREEDQKKTIKTLGENKEIQILNGRFGPYISFEKKNYK
                     IPKNVDPTGLTFEQCMEMIQEQAGTDKKGKKGNTRKTASTEKSAKNTKTSKTTKTTKT
                     SKTKKSTTAGKKKV"
BASE COUNT         2924 a         1825 c         1960 g         2848 t
ORIGIN      
        1 gtgaatggct gctctgtttc ccaacaatcc gattaatccg actgcaaaat cgcgatgata
       61 ggtcaaagtc caaactgaag ttccatcacc atgtaacact accggttgac ccttcaacat
      121 ccgatgtaat acagtgtaac cccctatggc cggcactttc ttatagttgt aggtatgcga
      181 aggacgaact atggtaaaag gaaagccgga tttttttgct tcacttttca atagtttttc
      241 acagaaaatt ttcttttggg agtattccca ataaggattc catagtggag ttttttcggt
      301 aattggcaat ttatcgggtg gcttctgata agccgatgca gtactgataa acacatattg
      361 agcggtacgg tctcgaaaca gtcggatatc ctgctcgata tgttgcggct caaaagcgat
      421 gaaatcgaca acggcatcaa aatgatgtcc tttcagctta gcctccgctt cattgccgtt
      481 gcgaatatcg gcatgaatgg ttgtaacttc tttcggcaca tggtccttat cggtcattcc
      541 acgattcaaa tgaaatactt ccatcccttt ctcaatggct atttccgtac atgccgaact
      601 gataatgcct gttccgccaa taaataaaac tttcatcaga atataattta ttttttaatg
      661 gtctgatgga acccacatgt ctgaatatta tattcattcg tgtttgtgta tgtaatacat
      721 gtgggtttca taaactttaa agtttttcgt ttaactcaac gaagttacag ttttttttgt
      781 aattaattaa agaaattttt ctttaattcc tttgtcgtga aaaaaaatag ttgtaatttt
      841 gcgacgtgaa aaacaaggct tttcagaaaa aagataaaaa caaacataac aattaatttt
      901 aaaaaaaatg gcaaatttag atttaagtaa gtacggtatt accggcgagt acgaaatcgt
      961 acacaatcct acttatgaac aactgttcag agcagaaacc gaccctaaaa acgaaggttt
     1021 tgaacgcggt gttgttacca caaccggtgc agtagcagta gataccggta tttttaccgg
     1081 acgttcgccc aaagataaat attttgtaaa agatgaaatt accgaaaaag ccatgtggtg
     1141 ggacggggtt attaatcgac ccatcaacac ggaggtttgg gactattgca gaggcttggt
     1201 aaccgacagg ctgggttctg tgaaaaaact ttatgtggta gattgtttct gcggtaccaa
     1261 tcccgatacg cgaatgaaag tgcgctttgt tatggaagtg gcttggcagg cacattttgt
     1321 aaccaacatg tttatccgtc cttcacacta tgaactggca aattttggcg aacccgattt
     1381 tgttgtgctc aatggttcta aggttaccaa tcctaaatgg aaggaacagg gattgaactc
     1441 tgaagttttt gttcttttca atctttctca acgtatggcc gttatcggtg gaacatggta
     1501 cggtggtgaa atgaagaaag gaattttctc actgatgaat ttttatcttc cacagaaagg
     1561 tatagcttcc atgcactgct ctgccaatgt tggcgaagac ggagatgtag ccattttctt
     1621 tggtttgtcg gggacaggaa aaacaactct ttctgccgat ccaaaacgct atttaattgg
     1681 tgacgacgaa cacggatggg atgatcacgg cgtattcaat tatgaaggtg gatgttatgc
     1741 caaagttatc gatttaagcg aagaaaacga accggatatc tggcgtgcta ttcgtcggga
     1801 tgctttgttg gaaaatgtta ccgttttgcc cgatggcaca cccgactttt caaaagcagc
     1861 ttctaaaacg gaaaatacac gtgtttctta cccaatttat tatatcaata aaattgtgct
     1921 tccttcaaaa gccggacacg caagcaaaat tatctatttg tcggcagatg cttacggcgt
     1981 tttgcctccg gtttctattt tggatgaaaa atcggcacag tatcacttcc tttgcggata
     2041 tacttcaaaa ttggcaggaa ccgaacgcgg tatcaacgaa cccgttcctt ccttctcgcc
     2101 tgcatttgga gaagcatttt taacattgca tcctaccatt tatgccaaag tgttggccga
     2161 aaaaatgaaa aagcacaatg caaaggctta tttggttaat accggttgga atggtactgg
     2221 caaacgtatt tccattaagg acacccgggc cattatcgat gccattattg acggttcaat
     2281 cgaaaaagca gaaaaagttc atattccaat tatgaatctg actattccaa aatctttgaa
     2341 caatgtgaac cctgctattc ttgatccgcg cgacacctat gctaatccgg cagaatggga
     2401 acagaaagca aaatctttgg ctgctcaata tatcaagaac tttgaacagt atctcgataa
     2461 cgaaaatgcc aaagaacttt tatctgcagg ccctcaactt taataagtta catacctgaa
     2521 atataaaaag ggagccgttc atttttgagc agttcccttt tttcttctaa aaagtagaga
     2581 aagaagttat ttatattgat ttgaaaccga ttatttcgcg aacatcgcga atggttttcc
     2641 gcgcattttc ctgtgctttt tcttttccca tgcgggcaac attcgacagg taatctttat
     2701 tgctcagaag gtctttaatt ttttcacgaa acggagcagt aaaagtaatc atatcttctg
     2761 ccagctgttt tttcatttcc ccgtatttga tttggcaaga atcgtattgt tcttcaaaat
     2821 aagccaaagt ttcggcactg gatacggctc tcatcaattg aaaaagattt tcgatgggtt
     2881 gagatttttc ctggtgaggc tgtgtggggc ctgcatcggt taccgctttc attacttttt
     2941 tccgaattgc ctccggctcg tccgataaga aaatggcatt gccttctcct tccgattttc
     3001 ccatttttcc cgtaccgttg agtcccggaa ttttaatcag ttcgtttccg aaattaaaag
     3061 cttgcggttc agggaaataa tctacctgat acattctgtt gaatcgattg gcaaaagtcc
     3121 gcgtcatttc gagatgttgt tcctggtctt tgcctaccgg aaccttgttg gcgcgatgca
     3181 gcaaaatatc ggcagccatc aatgtcggat aagtcaacag tccggcattt acgttgtggg
     3241 gttgctggcg aactttatct ttgaaggaag tacagcgttc caattcgccg acgtaagcat
     3301 tcatgttcaa gaaaagatac agttccgtaa cttcaggaac atcactctgt atgtaaataa
     3361 ctgctttttc gggatcaagg cctgcagcca aatattccgc taaaatttct ctcacatttt
     3421 ccgtcagatt ttgcggtgtg ggatgcgtgg tcagtgagtg gtaatcggct ataaaaaaat
     3481 aacaattgta ttcattttgc atttgtacaa aattcttcaa tgctccaaaa tagtttccca
     3541 aatgcaagtt cccggtcgga cgtatgccgc taagtacagt ttccatcaaa aataaaaaat
     3601 tttagtgttg tattttaaat tcaaatgcaa aattaatcat tttaggagaa atatccagct
     3661 tctcaattaa agtcagagga atttgattcg gagagaagca aaagaatttt tccaaaaaac
     3721 gttggaaata taaatctttc tttgcatttg attatttttg cagaaaaatt tttaaaaatg
     3781 gagatgtaat atggagaaga aagtaatatt aataaccgga gcaagcagcg gtattggatt
     3841 atcaacagcc attcaactaa tgaacaaggg ttacagggtt tatgctgcct ctcgtagtgg
     3901 tggtgaaaat agcacatcca aaaatggcgg agaaattatt tctacaaaaa tgaatgtgaa
     3961 tagtgaagaa gaaataaaaa aagtgttgga cgagattttg gaacaaaatg ggcatttgga
     4021 tgctgttatt tccaatgcag gtaacggcat agccggttct atcgaagaca cttccattga
     4081 agaagccaaa tatcagtttg aaacgaattt ttttggagca gtaagagtta ttcaggcctg
     4141 tttacccatt ttcaggaaac agggctatgg aaaaatcatt gccacatctt cggtagctgc
     4201 cattgttcct attccttttc aggcatttta ttccgcttcc aaagcagctt tgcttatttt
     4261 tatgcaggcg cttgacatgg aaaccaaacc ttttggcatt cagtgcagca ccattttacc
     4321 gggtgatacc aaaaccaatt tcacgtcggc aagaaaatat accgcagctt ctcaatcgac
     4381 cgaatcggtt tattacgaaa aaatgaaaaa agctgtcgga aaaatggaaa aagatgaaca
     4441 aaacgggatg cccgcagatg ctattgcgaa agccattgtc aagcaaattg tacacaaaag
     4501 catgaaagca atcgtagttc cgggatttca gtatcaggtt atttgctggc ttttcaaccg
     4561 attgcccgtc aaattcaggc tgaaagtggt aagtatgctc tattagttcc tgaaagtttt
     4621 gctgatacgc tgaaagtaca ttaaacgatt aaaaaaaagc ttgaaatact aaaaactgca
     4681 aggcgagaga ttttatttcg ccaaaataag ctgttttttt tcgttttcct ttatttgatt
     4741 gttgtcgagc ataaagcgga taacctgcaa aacctttttt tctttaaaat gaattttttg
     4801 aacaactata tcgaggggaa gattttcgtt tgagagtact tgaacaattg acgaacgaat
     4861 ggcttcaaat tcggcagtag aaatttccgt ttctttcttc ttttgacaca cgtcgcattt
     4921 gccacaaggc tggctgtttt tttcgccgaa gtaggaaagc aacatttgac ttcgacagac
     4981 attttcttct tgggcatagt tcagtacact ctgcacgcgg gaaacaaatc gctctcgtcg
     5041 gtcttcataa gcttcacggc ttaaatatat ctgtttggcg tccacccttt cctgtgtaaa
     5101 agtaagaaat gccgtttttt ttcttggtat gtaacggata actttttcct ttgacagttc
     5161 tactaactgt tgatacactt cctgaggggt agattgaagc cgttgggcaa cgacttcttc
     5221 atgaatgaaa gccggctccg tgaaaagtcc cgtgtaagaa cgtaaaagta tgtgtataag
     5281 cttttcttgc ttcggagttt gctttagttg atacaattcc tcttttttaa ccgtgaaaag
     5341 cacttgtgat gtactttcct cttcatcggt aaattcaata taaccggctt gctgaaggat
     5401 tttcaggctg ctgaaagcca ttaatacagg caagtgaaat ttagtgcaaa aatcgaccag
     5461 atcgaaaggg aaagtattgt ccagtcctga gccaatacca acctgataat aattgcccaa
     5521 agattcgtaa actttgcgaa tcatttcttt tggcggaaaa gtatccgaaa tgcgtttttt
     5581 aatttttgct gcatcgctgt tgttgtaaag cagaacggca aaagcttttt tttcgtctcg
     5641 gcctgctctt ccggcttcct gaaaatatgc ttccagcgaa tcgggcaaat ccaaatgaat
     5701 caccgaccgt acatcggctt tgtcgattcc catgccaaaa gcgttggtgg aaacaatgac
     5761 gcgggttttc ccgtttttcc agcgtgtttg aatttcgtcc tttttcacgt tgttgaggcc
     5821 tgcatgataa tgttcggcta caattccgtt ttttacaaga aactcggctg tttcttttgt
     5881 tttttttctg tttcgcacat aaactaccga agtgcccggt acactgttga gaattttcaa
     5941 caaatactga tttttatctt cggtatatcg aaccacataa gccaaattgg aacgataaaa
     6001 actttttttg aaaatgttga atgccggaaa atgtagttgt ttctgtatgt cttcggcgac
     6061 ttcaggagtg gcagtagccg taagtgcaag tacgggtgca ttgggtaaaa acgctctgat
     6121 ttcggcaatt ttcaggtagg aaggtcgaaa gtcgtaaccc cattgagaaa tacagtgcga
     6181 ttcgtccaca gcaatgagac aaacttttgt ctgttttatt ttttcgataa aaatgcgtgt
     6241 tgcaagtctt tcgggcgata cataaagaaa tttgtaggct ccaaatgaag cattttcgag
     6301 cgtcatcaga atttcgtcgt aagacatgcc agagaaaatg gcagcagccg caattcctct
     6361 ttttttcaag ttttctacct gatctttcat cagtgcaatc aagggtgtaa caaccacgca
     6421 aactccatcc attgccatgg caggcacctg aaaagtcagc gattttcctc cgccggtagg
     6481 cataagtccc aacgtatcgt ttccggaagc tatgctacca ataatttcat cctgtaatgg
     6541 tcgaaatgaa gaatatcccc agtattgctt gagaatgtca aggtatttct gcataaatta
     6601 attgcaactt tattacaaaa ataatgattt ttaccgaaag ttccgatttt tatatgtaaa
     6661 tcaggaaaga gaaaaaaagg tgaaaattgt tcactaaatt agattatttc cttttattca
     6721 atgaagtgaa cattttgtat atttcagttc gttttatgtt ttcgttttat aaattttaaa
     6781 aataataatc tgaaaatttg gctgatacct ttcaaatttt gttactttgc aggcaatttt
     6841 aaataccgcg tattaattta aagttgtagt atgccaaaaa accttgttat tgtagaatct
     6901 ccggccaaag ccaaaactat cgaaaaattt ctgggaaaag aatatcatgt tgtttcaagt
     6961 tacggacata ttcgtgattt atccgaaaaa ggcattggta ttgattttga acacaattat
     7021 cagcctcaat acaaagtttt acccgacaag aaaaaactgg tttcggaatt aaagaaagcc
     7081 actgaacagg cagagttggt ttggctggca tctgatgaag accgcgaagg agaagctatt
     7141 gcatggcatt tgtcggaaga actgaaactt ccaaaagaaa agacacgcag aattgtattt
     7201 catgaaataa cgaaagatgc cattgttcac gccattgaac atccgcgaga tttggatttg
     7261 aatttggtag atgcccagca ggcgcgtcgt gtcctcgacc gaattgttgg ttttgaactt
     7321 tctccgctgt tatggaaaaa aattcgtcct tccctttcgg cagggcgtgt tcagtcggta
     7381 gctgtccgtc tgattgtgga acgcgaaaga gaaatcaatc agtttgttcc cgaatcgtct
     7441 tatcgcgtaa cggcaatttt cagtacaaca gacaaagatg gtcgttccgt agaattgaag
     7501 gcagaacttc aacagcgttt ttccaccaaa gaagaagcat tgaatttttt aaatcaatgt
     7561 aaggaagcaa tttttcgggt ggaaaatatt actaccaaac cgaccaaaag atcacccgca
     7621 ccgcctttta ccacttctac tcttcagcag gaagctgcac ggaaactcgg tttttcggtt
     7681 tctcaaacca tgttgatagc tcagcatctt tacgaatcgg gaaaaataac ctatatgcga
     7741 accgattcgg tgaatttgtc ggattttgcc ctgaaggcgg cagaagcgga aattaccaac
     7801 atgttgggga aaaaatattg gaaattgcga cattatgtta caaaaaccaa aggcgctcag
     7861 gaagctcatg aggcgattcg tcctacttat atgaatgttc acgaaattga tggttcagac
     7921 caggaaaagc gattgtatga cctgatatgg aaacgtacgc tggcttcaca aatggcggat
     7981 gcggaatttg aaaaaaccac cattcaaatt ggaatgcaag gtcaacctta ccagtttgtg
     8041 gcttcgggtg aagtgattat atttgatggt tttcttcatc tttacagcga atcgacagat
     8101 gatgagtccg attcagaaaa atcggaaatt ttaccggcca ttaatgtggg cgaaaaattg
     8161 ttgccaaaag aaattctcgc tcaggaacgt tttacccagc gtccgccccg atatacggaa
     8221 gcaagtttgg tgcgaaaact cgaagaattg ggcattgggc gaccttctac gtatgctcca
     8281 actatttcga ctattcaaaa tcgaaaatac atagaaaaag gaaaccgtcc gggcgaaaaa
     8341 cgaacttaca atgtgctgaa gcttgttgac ggaaaaattt ccgatcggtc ggctacggaa
     8401 atcaccggtg ccgaaaaagc aaaattattt ccgactgata tcggtatagt ggtcaacgat
     8461 tttttaattg aatattttcc cgggattctg agttataact tcacggccaa tgtggaaaag
     8521 gaatttgata atgtggcggc gggaaaagta ggttggacgc aagttatcga caagttttac
     8581 aaaaaatttc atccaacggt tgaaaaaacc ttaacttcca ccgagcgaaa agttggcggc
     8641 cgcacgttgg gtaccgatcc caaaacaggg aaaccggtta cggttaaaat aggaagatat
     8701 gggccttatg ttcaaattgg aacggccgaa gatgctgaaa atcctgtttt tgcttcgctg
     8761 cgtccgggtc agtccatcga cagcattaca ctcgaagaag cgctcgattt gtttaaactc
     8821 ccgcgtgaag taggggaatt tgaaggaaaa ccgctgactg ttgccattgg acgttttggt
     8881 ccttatatcc gtcagggaaa ttcgttttat tccataccga aaggggaaga tccgttgaat
     8941 gtttcactgg aacgcgccat agaaattatt caacacaaac gggaggaaga tcagaagaaa
     9001 actatcaaaa ctttaggcga aaacaaagag attcaaattt taaacggacg cttcgggcct
     9061 tacatttctt ttgaaaaaaa gaactacaaa attcctaaaa atgtcgatcc tacaggttta
     9121 acttttgaac aatgtatgga aatgattcag gaacaggccg gaacagataa aaaagggaaa
     9181 aagggaaata cgaggaaaac agcttcgacc gaaaagtctg caaaaaatac aaaaacctcc
     9241 aaaacgacta aaacgaccaa aacatcaaaa acaaaaaaat ctacgactgc tggcaagaaa
     9301 aaagtttaat aaaaaattca ggaaacattt ctgggcgcaa gtaggaaaaa tagcagagga
     9361 agaaaaaacc ggtttttttc ttcctctgct atttttttgt ctgtatgaga aaaaattcat
     9421 ttttttttgg agaataatgg aaatattttt tgcaaaatac tattagctgt ccttttaaac
     9481 acttaatcag accaataaaa ttcttttgaa gaaacattga aatttttatc aatgaaacaa
     9541 aaatacatca caatgaa
//
LOCUS       DAOTAX010000063         9236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_6376_length_9236_cov_29.732164, whole genome shotgun sequence.
ACCESSION   DAOTAX010000063 DAOTAX010000000
VERSION     DAOTAX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_9236_cov_29.732164"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1452
                     /locus_tag="PLM04_06955"
     CDS             52..1452
                     /locus_tag="PLM04_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HON02847.1"
                     /translation="MEKTSEEKKSFQWKDVFQKIISDLKTLTNLHEDTNIESTIASIK
                     KDVEFKGANVWILFFAVIICSVGLNVNSTAVIIGAMLISPLMGPINGVGLSIGIYDTE
                     LLRLSLRNLLIMILVGLVASTFYFLITPITDAQSELLARTRPTLFDVFIAFFGGCAGI
                     VAASRKEGKITVISGVAIATALMPPLCTAGYGIASGQLRFFFGAFYLLFINSFFIAIA
                     TFLVTHLLHFPVKQFVDIQRQKVVKRSIIIFSILTIIPSFFLAWNVVSETRFNSHTYR
                     YIQDIQQLDLFSEKEIINVSRKYSRKEKSLTLSVIGKPLTEEQIRYIQQLLPKYGLNN
                     VNLIIKQTGGTFDLGAQTAFIEKLLMDKDIQLQKKDSIIAALNNKLQLMQTSGVNELQ
                     LAREVKVQFPDIKQIAFSNSIFINLDHLTQDTVPTIFIQWEQTPPNKKALLSQMNEWL
                     KVRLNLEKLKIIEVEM"
     gene            1498..2211
                     /locus_tag="PLM04_06960"
     CDS             1498..2211
                     /locus_tag="PLM04_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HON02848.1"
                     /translation="MKIDVCFSPALYPFYSSGNSKIAVIVDVFRASTTICKALMEGAS
                     AIFPVASLEKAEELKSRGYFVGAERNVEKCDFADAGNSPFDYYSEKVKGKEIVFTTTN
                     GTRAIELAAAENEVIIGAFCNISAVSDFCRNARKDVLVICAGWNNLFSLEDTLFAGLL
                     VHRLKCSGNFETSGDSAVAALVLWQQAEPDIKTFLHQSEHAKRLFTHHLEKDFDFCLA
                     QDTLPMVPFYDKSSQKILF"
     gene            complement(2968..3411)
                     /gene="rplI"
                     /locus_tag="PLM04_06965"
     CDS             complement(2968..3411)
                     /gene="rplI"
                     /locus_tag="PLM04_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HON02849.1"
                     /translation="MEVILKEDVNNLGYKGDIVKVKDGYARNYLIPTKKAVIATDSAK
                     KMLEEEKKQRAHKLERIKNEALELAEKAKSINLVIEAKASSTGKIFGAVGPIQIADAY
                     EKAGLVIDRKVIVVDEPVKELGSYKATLKLHREVSVEISFEVIGV"
     gene            complement(3432..3704)
                     /gene="rpsR"
                     /locus_tag="PLM04_06970"
     CDS             complement(3432..3704)
                     /gene="rpsR"
                     /locus_tag="PLM04_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HON02850.1"
                     /translation="MATNNNEEIRYLTPPTVDIKKKKYCRFKKSGIKYIDYKDPEFLK
                     KFVNEQGKILPRRITGTSLKYQRRVAQAIKRARHLALLPYVTDMLK"
     gene            complement(3708..4052)
                     /gene="rpsF"
                     /locus_tag="PLM04_06975"
     CDS             complement(3708..4052)
                     /gene="rpsF"
                     /locus_tag="PLM04_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HON02851.1"
                     /translation="MNHYETVFILTPVLSDLQMKEAVEKFKTILQENGATITNEENWG
                     LRKLAYPIEKKSSGFYCLLQFDAEPSVVAVLETNYRRDERVIRYLTTRLDKYAYEYAE
                     KRKNVKSKEGKE"
     gene            4384..4986
                     /locus_tag="PLM04_06980"
     CDS             4384..4986
                     /locus_tag="PLM04_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HON02852.1"
                     /translation="MSKVLYIKANAKPEGESRTFMIADKFVEAYKKHHPQDEIITLDL
                     YKEDIHFLTVEDVMIHMSNEVEETENAMKKYALQFKSADKYIFAEPMWNLGVPAILKA
                     YIDYVSIPNITFKYGENGPIGLCQGKKALNVITSGGDYSEGMAAEWDLCDKYLKTIMG
                     LFGVLDYTTIRASNLDVIGMDVDAIVNKAIKEAEELAKTF"
     gene            5108..5377
                     /locus_tag="PLM04_06985"
     CDS             5108..5377
                     /locus_tag="PLM04_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HON02853.1"
                     /translation="MKSGIHPTNYRPVVFKDLSNGDTFITRSTINTKETIEIDGVTYP
                     LVKLEISSSSHPYFTGKAKLVDSAGRVDKFMNRYAKSLEKKKTQK"
     gene            complement(5435..6085)
                     /locus_tag="PLM04_06990"
     CDS             complement(5435..6085)
                     /locus_tag="PLM04_06990"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HON02854.1"
                     /translation="MKLINNFFTVIFFFLCAKISAQGIDKLYVGMPDALHPTLTSQQR
                     LELIEYAKAGQNDTTLNRYKKPVFLLKLDTLHQHLMVKNSGSTSFEMKVLDANSNEPM
                     IAIIRTVCAPICHSLIEFYDFKWNRLSVTFPYPQATDWLDLEKAQKTEEGVETFQKIL
                     KYTFVSLSFQEEGQIVMAFNNSIDFLSDEDKKLVFPCMNTAPLLFQWSDLKWIREK"
     gene            complement(6069..>9236)
                     /locus_tag="PLM04_06995"
     CDS             complement(6069..>9236)
                     /locus_tag="PLM04_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HON02855.1"
                     /translation="LNKILSNNQFGLFSFNFNTKGEKQANKNFQGTIEADIPEFQWNG
                     YSYRDMKLKGNYDGSGFDGTFDVADENIDAHFTGIVDMTKRLPVFDFSLNLAKANLNA
                     LKLTTKYSNANLSFDARTNMVGNSLDNLNGFVLFNNIAFTNRNRTLNAGKIQFVSRTE
                     NNETYFLISSDYVNGSISGNFIYSSLDQTFKRLAYQYLPVFSDKVPHENSHPNFINVD
                     LKISTTGELTDILELPFQLKGNSYIKGYYDEKIYKVDLISNFPYFKFGRREFDNIFLS
                     LQNEEDKLSFVTRAQMDGKNKPMHFYINATAADNAVNTLLGWQNSDSITNAGEFKTQT
                     KFATTDGKINAQISVLPSQIILMDSVLNIFPSVVEYKSDGSLQINDFKMDGKSQFLHV
                     NGIASKNEKDSLQFAMNNLDLYFIMNDLVKLKDPSLGGYFTGNATMYSAFQQPFFEAK
                     LLVKDFSMNQSVLGDVNLYSYWDKEKKEVVSNAHVLNNKDTVAYIDGVYVPGKDSIDF
                     VYDMHHLNMGMLKRYFGDVVQNVRGFATGKLRMFGPTKKMRFDGDLYVAEGQLKMALT
                     QTTYFFNDTIRMRPTSVEFNNIKMYDEEGNEGLLDGIMKHTGMFGDLKYDVMIRSNNI
                     LALNTHANANEIFYGKAYAGGTVHVFGDVNVTNVWVDMVSRPGSKCYVTMPGSSTAAD
                     NSFVLFVNKNDTIKTQPDEQKGKKLPENTYNTKVNLQIGITPDAEIQFIIDPKAGDML
                     VGKGTGNLRFEFDTYSDIKLFGTYTIEKGNYLFTLQNVIRKEFKIDEGSTLTWTGDLY
                     NAQVKIKGIYSLMASLRDLMDESQLSSLPRTTVPVNCILLLSGNLMKPNVEFDIDLPS
                     SDESVKQMVKSIISTNEMMARQIIYLLIFNKFYNPYLQSAANAPVGIGSSEAVSLVVS
                     TLSAQMNNWLAQMINSNIISIGFDYRQTDQLSSDVQAQFLIQPNNRLIINGNLGYRND
                     NVNLSNNTNKFIGDIDVEWLLTEGGKFRLKAYNHTIDRYQLRSAKNTQGIGLIYKENF
                     SNFDELFRYYWNILTFKKKNNEADQ"
BASE COUNT         2869 a         1669 c         1655 g         3043 t
ORIGIN      
        1 gataagctca cttttttatt ctacgacaaa tatttacaat gatgacaatt tatggaaaaa
       61 acttcagaag aaaaaaaaag ttttcaatgg aaagatgttt ttcaaaaaat aatatctgat
      121 ttaaaaacac ttaccaattt acacgaagac acaaatattg aatctaccat agcttccata
      181 aaaaaagatg tagaatttaa gggggctaat gtctggattt tattttttgc tgttatcatc
      241 tgttccgtag gactcaatgt taattctaca gcagtgatta tcggtgccat gctcatttct
      301 ccgcttatgg gacccatcaa tggagtagga ttgtcaatag gcatttatga tacggaactc
      361 ctgaggttat cattgcgaaa cttgcttata atgatattgg taggattggt tgcctctacg
      421 ttttattttc ttattactcc catcactgat gcccaatcgg aacttttagc gcgaactcgc
      481 cctactttat ttgatgtgtt tattgctttt tttggcggat gtgccggcat tgtggctgct
      541 tctcgaaaag aagggaaaat aactgttatt tcaggtgtgg ccattgctac tgctcttatg
      601 ccccctttat gcacagccgg ctatgggata gcttcgggac agctacgttt tttcttcgga
      661 gcattttatc ttctctttat caactctttt tttattgcta tagctacttt tttggtaact
      721 catttacttc attttccagt aaaacaattc gtcgacatac aaagacaaaa agttgtaaaa
      781 cgttctatta ttatattttc cattttaaca attattccca gtttttttct ggcatggaat
      841 gttgttagcg aaacaagatt caattcacat acctatcgtt acattcagga tatccaacaa
      901 ctcgatctgt ttagtgaaaa ggaaatcatc aacgtttcga gaaaatattc aagaaaggaa
      961 aaatctctta cactttctgt catagggaaa cctttgaccg aagagcaaat ccgatacata
     1021 caacaattac ttcccaagta tggtttgaat aatgtaaatc ttattatcaa gcaaacaggg
     1081 gggacgttcg acttaggtgc tcaaactgca tttatcgaaa aattactgat ggacaaagat
     1141 atccaacttc aaaaaaaaga ttcaataata gctgctttaa acaacaaatt gcaactgatg
     1201 caaacttcag gggttaatga gttgcaactt gcccgtgaag tgaaggtgca atttcccgat
     1261 ataaaacaaa tagccttttc aaattcaatt tttatcaatt tggatcattt aacacaagat
     1321 actgttccca ctatatttat ccaatgggaa caaacacctc ccaataaaaa agcattattg
     1381 tcccagatga atgaatggct gaaagtgcga cttaatttgg aaaaattaaa aataatagaa
     1441 gttgaaatgt agttttcaat ttttttacct ggtttcttgt aaaaaacttt attccccatg
     1501 aaaatagatg tttgtttttc gccggcatta tacccttttt attcgtcagg aaattcaaaa
     1561 attgcagtaa tagtggatgt tttcagggcg tcgactacca tttgcaaagc tttgatggaa
     1621 ggagcatccg ccatttttcc cgttgcgtcg ctcgaaaaag ccgaagaatt aaaaagtcgt
     1681 ggttattttg tcggagcgga gcggaatgtg gaaaagtgcg attttgcaga tgccggtaat
     1741 tctccgttcg actattattc cgaaaaagtg aaggggaaag aaattgtatt tactacaacc
     1801 aacggaaccc gtgccattga actggcagca gccgaaaacg aagtaattat cggggctttt
     1861 tgcaacatta gtgcagtgtc cgatttttgc cgcaatgccc gaaaagatgt acttgttatt
     1921 tgtgccggct ggaataatct tttcagtttg gaagatactt tgtttgcagg cttgctcgtc
     1981 cataggttga agtgcagcgg gaatttcgaa acttcaggtg attcggcggt tgctgcatta
     2041 gttttatggc aacaggccga gcccgatatc aaaacgtttt tgcatcaaag tgaacatgca
     2101 aaacggttgt ttacgcatca tttggaaaaa gattttgatt tttgtctggc tcaggatacc
     2161 cttccgatgg ttccgtttta cgacaagtct tctcaaaaaa tattatttta acgtgaggtt
     2221 cggcataaga atatttaata agcaattata aataagctaa taaggctgaa aggaagtatt
     2281 cccatgttac taagtctggt cgtttctata taaagtcatt ctatccaaaa acgaggctta
     2341 tttttaaata aaaccttagt aacgataatt aataacaaga ataaacctta ttaattatca
     2401 attttttata tcaaaacttc actaaaaatt cgttcgccta ataaaacgct aatcaatggt
     2461 ttaaataaaa aagtttcaca tgaaaaacat agatgaaggg gtgattagaa gaacttattt
     2521 tccccagttg cgaagatatg tgtagtactg agaaccacaa ttctgaaaga acttctcgaa
     2581 ggattttaat tggctaaagt ttaacttgtc tttgtttgaa aggaaattaa ccatagaaag
     2641 caaaggcaat agttattccc ttttcaatat catcctgtaa aacaatgata aacaatgttc
     2701 atttgattgt aagatttata tacttgtcct acgtacaaac gaacagaact ttaaatgatg
     2761 aatttgctaa agaaattatt gactttatgc aattgcggca tgaatcctgt ttttaacgaa
     2821 cttttgtgta aacataccaa tgtaaattgt gaattttgaa tttcatggga tggaaaatat
     2881 ttgttgatat gaatcaataa aaaacgaaag gcatctcatt gaacagagac accttccgtt
     2941 aaaaatacag tttattcttt cagattatta aacgccgatt acttcaaagg aaatttcgac
     3001 gctcacttct ctgtgaagtt taagcgtagc tttataggaa cctaattctt tcaccggttc
     3061 gtccacaacg attactttgc gatcaataac taagcctgct ttttcataag catcggcaat
     3121 ttgaatcgga cctacggcac caaaaatttt tcctgttgaa ctcgctttgg cttcaataac
     3181 caaattaata cttttagcct tttctgccaa ttcaagcgct tcgtttttaa tgcgttccag
     3241 tttatgagca cgttgtttct tttcttcttc gagcattttc tttgccgaat cggttgcaat
     3301 aacggctttt tttgtgggga taagataatt acgcgcgtaa ccatctttca cttttacgat
     3361 atctccttta taaccaagat tatttacatc ttcttttaaa attacttcca ttttttgtcc
     3421 tcctgattaa attatttcaa catgtctgtt acatagggaa gcaacgctaa atgacgggct
     3481 ctttttatgg cctgcgcaac tctacgttga tactttaaag atgtgccggt gatgcggcga
     3541 ggaagaattt ttccctgctc gtttacaaac tttttcaaaa attccggatc cttgtaatcg
     3601 atatatttaa taccactctt tttgaatcgg caatattttt tctttttgat atcaaccgta
     3661 ggaggagtta aatatcttat ttcttcgttg ttgttggttg ccatagttta ttcctttcct
     3721 tctttagatt ttacattttt tcttttttct gcatactcgt aggcatattt gtccaaacga
     3781 gtcgttaaat aacgaatcac ccgttcatca cggcgataat tggtttccaa aactgccact
     3841 acggacggtt cggcgtcaaa ctgcagcaaa caataaaaac ctgaagattt tttttcgatt
     3901 ggataggcca gcttgcgtaa gccccaattt tcttcattgg taattgttgc accattttcc
     3961 tgcaaaatgg ttttaaattt ttctaccgct tccttcatct gcaggtcaga taaaacggga
     4021 gttaaaatga aaacggtttc ataatgattc aacatactgt ttaatagctt tttatgttta
     4081 taaattttat ttgttaaagc ggttacaaag gtaatgaata tttttgattt tcaaaagaaa
     4141 tgtttttatt aaaatgttga atacataggg ttgagatttt tagaaacaat ttttttcccc
     4201 ttattccgaa tttttttgac aaacgaaaga attctattat ttttgtctta aatcatcccc
     4261 gctgtaaata gaaacagctg aattgaatct ttttttaatt tttctaaaag cagtcaaatc
     4321 agagaaaata cgacaatggc ataagaattt gttaacaaca aaaaaatagg aggaaaattt
     4381 attatgagta aagttcttta tatcaaagcg aatgctaaac ctgagggcga atccagaaca
     4441 ttcatgattg ccgataaatt tgtggaagca tacaaaaaac accatcctca ggacgaaatt
     4501 attacgctgg atttgtacaa ggaagacatt cattttttaa cagttgagga tgtcatgatt
     4561 cacatgagca atgaagtgga agaaacagaa aatgcaatga aaaaatacgc tctgcaattt
     4621 aaaagtgccg acaagtacat ttttgctgaa cctatgtgga atttgggggt cccggctata
     4681 ttaaaagctt atattgatta tgtaagtatt ccgaatatca cttttaaata tggcgagaat
     4741 ggcccaattg gtttgtgtca aggcaagaaa gcacttaatg ttattaccag cggcggcgat
     4801 tattccgaag gaatggctgc agaatgggat ttgtgcgata aatatctcaa aaccattatg
     4861 ggactttttg gtgtattgga ttatacaacc atcagagctt ctaaccttga tgtgatcggg
     4921 atggatgttg atgcaatagt caataaggcc ataaaagagg ccgaggagtt ggcaaaaact
     4981 ttttaaaaat caaaagaata aatttttaat cgaaggggaa aaagaaaaaa gagaaaactc
     5041 tttgttgatt cagaaaaaaa tcgtatcttt gcaaactcaa aagaaaatac aaacatttaa
     5101 gacagatatg aaaagcggaa ttcatcctac caattatcgt ccggtagtat ttaaagactt
     5161 atcgaatggc gacacattta tcactcggtc gaccattaat actaaggaaa ccatagaaat
     5221 tgatggcgtt acttatccat tggttaaatt ggaaatttcc agttcttcgc acccttattt
     5281 tacgggtaaa gccaaattag ttgacagtgc cggtcgtgtt gataaattca tgaatcgcta
     5341 cgctaaaagt ttggagaaga aaaaaactca aaagtaattt ttttcataat aatgttgtgt
     5401 aaaaaggctt cagaaaaatt ttttgaggcc ttttttattt ttcccttatc cattttaaat
     5461 cgctccattg aaacagcaaa ggtgccgtat tcatgcaggg aaaaacgagt tttttatctt
     5521 cgtcgcttaa aaaatcgatg ctgttgttga aagccattac aatttgtcct tcctcctgaa
     5581 aactgagcga aacaaaagta tatttcaaga ttttttgaaa cgtttcaacc ccttcttcag
     5641 ttttttgggc tttttccaaa tcgagccaat cagtggcctg gggataaggg aaagttacag
     5701 aaaggcgatt ccatttaaaa tcatagaatt caatcaaaga atgacatatt ggtgcacaaa
     5761 ctgtgcggat aatggcaatc atcggttcgt ttgagttggc atccaaaacc ttcatttcaa
     5821 aagatgttga acccgaattt ttcaccatta agtgttgatg aagcgtatcg agttttaaca
     5881 gaaaaaccgg ttttttatag cgattgaggg tggtatcgtt ttgtccggct ttggcgtatt
     5941 cgataagttc gagccgttgt tgcgacgtca aagtcggatg aagggcatcc ggcatgccga
     6001 catacaactt atcgatgcct tgtgccgaaa tttttgcaca caaaaagaaa aaaatcaccg
     6061 taaaaaaatt attgatcagc ttcattgttt ttctttttaa aagtgagaat gttccaataa
     6121 taacggaaca attcgtcaaa atttgaaaaa ttttctttat atatcaatcc tattccttgc
     6181 gtattctttg ccgaccgaag ttgatagcgg tcaatggtgt ggttgtaggc ttttaatctg
     6241 aatttgccac cttccgtgag taaccattca acgtcaatgt cgccaataaa tttattggta
     6301 ttgttgctca aatttacatt atcatttcga taacccaaat ttccgttgat aattagccgg
     6361 ttatttggct gaataagaaa ttgagcctga acatcactgc tcaactgatc tgtttgacgg
     6421 taatcgaatc caatggaaat aatgttgctg ttgatcattt gagccagcca attattcatt
     6481 tgtgcactta aagtactgac aaccaatgat acggcttcgc tggaaccaat tccgacggga
     6541 gcgttggcag ccgactgcag ataagggttg taaaatttgt tgaaaatgag caaataaata
     6601 atttgtcgcg ccatcatttc gttggtgctg atgatgcttt ttaccatctg ttttacactt
     6661 tcgtcgctcg agggcaaatc aatatcaaat tctacattgg gtttcatcaa attaccactc
     6721 aacaacaata tacaatttac cggcacagtg gttcgaggca acgaactcaa ttgagattca
     6781 tccattaaat cccgcagaga agccatcaaa gaataaattc cctttatttt tacctgcgca
     6841 ttataaagat cgccggtcca agtcagcgta cttccttcat caattttaaa ttcttttcga
     6901 atcacatttt gaagggtaaa aagatagttt cctttttcga tggtgtaagt gccaaagagt
     6961 tttatgtcgg aataggtatc aaattcaaag cgcagatttc ctgttccttt tccaacaagc
     7021 atatcgccgg ctttcggatc gataataaat tgaatttcag catcgggcgt aatgcctatc
     7081 tgaagattta cctttgtatt gtatgtattt tctggcagtt ttttcccttt ttgctcgtcc
     7141 ggctgggtct ttattgtgtc atttttgttg acaaagagaa caaaactgtt gtcggcagca
     7201 gttgaagagc cgggcatagt tacatagcat ttggaacctg gccgtgaaac catgtccacc
     7261 caaacattgg taacattcac atcaccaaaa acatgaaccg tacctcctgc ataagctttt
     7321 ccgtagaata tttcattggc gttggcatgg gtattcaatg ccaaaatatt attggagcga
     7381 atcattacgt catacttcag gtcgccaaac attcctgtat gtttcattat tccatcaagt
     7441 aagccttcat ttccttcttc atcatacatc tttatattat tgaactcgac agaagtaggc
     7501 ctcatacgaa tggtatcgtt aaaaaaatag gttgtttgcg ttagtgccat ttttaattgt
     7561 ccttcggcca catacaaatc tccatcaaac cgcatttttt ttgtcggtcc aaacatccga
     7621 agtttgcccg ttgcaaatcc tctaacattt tgaaccacat caccaaaata tcgtttcaac
     7681 attcccatat tgaggtgatg catgtcatac acgaaatcaa tggaatcttt tcccggcaca
     7741 taaacgccgt caatgtaggc taccgtatcc ttgttgttta agacatgagc atttgaaacc
     7801 acttcttttt tttctttgtc ccaataggaa tacaaattca catctcccaa aaccgattgg
     7861 ttcatggaaa aatcctttac caataattta gcttcgaaaa aaggttgctg aaatgcgctg
     7921 tacatggttg cattgcccgt aaaatatcct cccagcgacg gatccttgag ttttaccaaa
     7981 tcgttcatga taaaatacaa atccaaatta ttcatggcaa attgcaagct gtctttttca
     8041 ttttttgaag caatgccgtt tacatgaaga aattggctct taccatccat tttaaaatcg
     8101 tttatttgaa gcgaaccatc ggatttatat tcaacaactg acggaaaaat atttaaaacg
     8161 gaatccatga gaattatttg gctgggaaga acggagattt gtgcattgat ttttccgtca
     8221 gttgttgcaa attttgtctg tgtcttaaat tctcctgcat tggtaataga atcgctattt
     8281 tgccacccca acaaagtgtt gacagcattg tcggctgccg ttgcgttgat ataaaaatgc
     8341 atgggcttgt tttttccatc catttgtgcc cgcgtaacga atgacaattt atcttcttca
     8401 ttttgaagag ataaaaagat attatcaaat tcccttctcc caaatttgaa ataagggaaa
     8461 tttgaaatta gatcaacttt ataaattttt tcgtcgtaat atccttttat atacgaattt
     8521 cctttcaatt gaaagggaag ttccaaaata tcagtcagtt cacctgtagt ggagattttc
     8581 aggtcgacat tgataaaatt cgggtgtgaa ttttcgtgcg gaactttatc ggaaaagacc
     8641 ggcaaatatt gatatgccaa tcttttaaaa gtttgatcta atgagctgta aataaaattt
     8701 cccgaaatac ttccgttcac gtagtcggaa gaaatcaaaa aataggtttc gttattttct
     8761 gtacgagaaa caaactgaat tttgccggca ttgagtgtcc gatttcggtt ggtgaaagct
     8821 atgttgttga ataaaacgaa tccgttgaga ttgtccagtg aatttcctac catgttggtt
     8881 cttgcatcaa aggacaaatt ggcattggaa tacttagttg tcagtttcaa cgcattcaaa
     8941 ttggctttgg ccaaattcag gctgaaatcg aataccggta aacgttttgt catatcgaca
     9001 attccagtaa aatgagcatc gatgttttcg tcggcaacat caaaagtccc atcaaatcca
     9061 cttccatcgt aatttccttt taatttcata tcgcggtagg aataaccatt ccattgaaat
     9121 tcgggaatat cagcttcaat agttccctga aaatttttat tggcctgctt ttctcctttg
     9181 gtgttaaaat taaatgaaaa aagtccaaac tgattgttgc tcagaatttt attcaa
//
LOCUS       DAOTAX010000064         9095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_6504_length_9095_cov_33.544580, whole genome shotgun sequence.
ACCESSION   DAOTAX010000064 DAOTAX010000000
VERSION     DAOTAX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_9095_cov_33.544580"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1032)
                     /locus_tag="PLM04_07000"
     CDS             complement(220..1032)
                     /locus_tag="PLM04_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HON02856.1"
                     /translation="MYELFDIKDKVIAFTGGTGVLGESMVKYLAAHGAKMAVLARSKE
                     KGDKLVEEVKGKGGEAMFLVTDVTNETILRQNVEDILAKYGKVDVLINAAGGNMPGAT
                     IGPNNTIFDLKLDDFRKVVDLNLMGSVIPTIVFAECMVKANRGNIINFSSASALRPLT
                     RVAGYGAAKAAVTNFTKYMAGELATKFGENFRVNAICPGFFITEQNRTLLINPDGSYT
                     DRGKAVIAHTPFRRFGVPEDLLGTMHYLVSDASKFVTGTVAIVDGGFDAFCI"
     gene            complement(1097..2344)
                     /locus_tag="PLM04_07005"
     CDS             complement(1097..2344)
                     /locus_tag="PLM04_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HON02857.1"
                     /translation="MKLGKYSFGVGDRFSHQGKAQLKALMKANEKGLNIIPVWNKSNR
                     EHTYVHSQPKDTRVEADNAVKALNYKGPYFVDADHINLNTVAPFVPVSDFFTLDVAAY
                     IGKESTKEEVNQFIDSCKKYMGQLEIPGLKEPLNVSKDLLEKITNKFLAATQQASEIY
                     AYLKKEKGADNFITEVSMDEVESPQTPVELFFILKMLADKGVPVQTIAPKFTGRFNKG
                     VDYAGDLEQFAKEFEEDVLVIDFAVKEFGLPEELKLSVHSGSDKFSIYPVMSRIIKKH
                     DKGLHLKTAGTTWLEEVIGLAKAGGDGLMAAKKIYANSYNRKDELCAPYADVIDIDGS
                     KLPSVEEVNAWGSEKFVNTLRHDQTCPDYNPNFRQLIHVAYKVAAEMGDEYISLLEKY
                     EEIIGQCVEENIYDRHLKRLFDL"
     gene            complement(2372..3778)
                     /gene="uxaC"
                     /locus_tag="PLM04_07010"
     CDS             complement(2372..3778)
                     /gene="uxaC"
                     /locus_tag="PLM04_07010"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HON02858.1"
                     /translation="MQSFINDDFLLQSDAAKELYHQHAEHQPIIDYHCHLNPEYIAND
                     RQFDNLGQIWLEGDHYKWRAMRSNGIDEKYCTGKDTSDWEKFEKWAETVPYTMRNPLY
                     HWTHLELKQAFGVDKLLSPATAKEIFDECTAKLRTKEYSARGLMKKFNVEVVCTTDDP
                     VDSLNYHIALKQEGFGIKVLPAWRPDKAMAVENPVEYRKYVEKLSEVSGVTIRKFDDL
                     LQALRVRHDFFADLGCKLSDHGIEEFYAEKWSKSEIEVIFNKVYGGKELTQEEIRKFK
                     SAMLYEGAVMDWEKGWTQQFHYGAIRNNNSRMMKQLGPDTGFDSIGDFTVAKAMSAFF
                     DSLDVSGKLTKTIIYNLNPRDNDLVATMIGNFQDGSVPGKMQFGAAWWFLDQKSGIEA
                     QLNSLSNLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLIGNDVENGLLPASEMDFL
                     GKMVENISYFNAKNFFEF"
     gene            complement(3942..5153)
                     /locus_tag="PLM04_07015"
     CDS             complement(3942..5153)
                     /locus_tag="PLM04_07015"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HON02859.1"
                     /translation="MFKISRSISLIVFALLIHNVYSQSVTVKNQSNVLLKDFMVEIPV
                     NKLPLTIGNYYVVSKEGVQLPVEIVSDINAEQIAVFSIERLQPNAILKFNFQRGQAEN
                     YPKRTYAEIAHKIGGQFVNGKYQGEFSWVKPNFIRLPGNFKDHSYFIKYEGAGWESDK
                     VAYRFYLDQRNALDAFGKKTPGIVLPAVGVDGYDNYHKMAVWGMDDMEVGKSLGIGSI
                     AYWDGKKAFRVEKRDSLVCCVISDGKVRSQIKTVYYGWEAANKVNLTSLITIDAGHRA
                     SHMELKIDGKLDNIATGIIKLKNTEIIVPPANDGEWTYYATFGCQSMNNDNMGLAIFY
                     RKRQLQTLTEDELNHVIVLTPDHGYVEYYFMATWEKEWQPIQDKASFINAIDDELNCL
                     NQKVSISINKK"
     gene            complement(5167..7053)
                     /locus_tag="PLM04_07020"
     CDS             complement(5167..7053)
                     /locus_tag="PLM04_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HON02860.1"
                     /translation="MKFSHSIFLFGAIVLCFPVKAQTVMINGVPRDTSYTVYSTYIKL
                     KKQYPFIRPAEARMSDNLKSYENIAYKSISPERKLLLNIYRLDNNEVLPAVIMVHGGG
                     WNSGSPSLQKAMAVKLAQKGFVCITVEYRLIPEALFPAGEEDLEDAVRWIADNAETYG
                     INRDKIAVSGCSAGGQLAALIGTKNKDKLIKAVVNIDGISSFIDKATIDRAQKARNDG
                     DKMPVDALWLGGTFAERPENWKVASAVTWVNQNSAPVCFINSSIPRFHNGRDEHIRML
                     DSLGIYSEVHAFDDCPHSFWHFHPWQLSTVQYVANFLNRILYNTPIAVDHSKYDLIVA
                     QDGTGDFRTVQEAINAVPDFRKRKTSIFIRNGFYREKLIIPETKDSLTLIGEDRNKTI
                     LSYNNFASKPNGFGDQLGTSGSASVYICSPNFTAENLTLENAAGPIGQAVAAIVRSDK
                     ARFLNCNFWGFQDTLYPHKAGSRQYYKNCYIEGTVDFIFGFSTAYFDSCELYCKESGF
                     ITAAATPQENNYGFVFYRCQIHGENPASFYLGRPWRPYAKVAFIECDMTNVIKPEGWD
                     NWGKVSNEKTAQFSEYQNSGEGGNLTNRRVKWSKTLSSRQVKNFDKEIVLGKDFFEKN
                     RENH"
     gene            complement(7146..>9095)
                     /locus_tag="PLM04_07025"
     CDS             complement(7146..>9095)
                     /locus_tag="PLM04_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON02861.1"
                     /translation="LIGQITDLDYMLNGDIVIENNNNASSYITFEGIGDDAVADGWGL
                     RIKNASNIEVRNLGFMNCDSEEGDDVSLQQNNDYIWVHNCDFFYGAAGSDADQIKGDG
                     ALDCKKSTYVTFSYNHFWDCGKCNLLGLSEGTTSGLFITYHHNWYDHSDSRHPRVRYY
                     STHVYNNYYDGNAKYGVGSTTGASVFVEGNYFRNCKYPMLISKQGTDIYQSSGTFSGE
                     DGGIIKAFNNYMTGQNRFVAYGSSDFANSTVDFDAYVVASRSETVPSTVKTKQGSNTY
                     NNFDTDASVMYQYTADTPEQAKEKVMQYAGRVNGGDLKWTFNNAVDDASYDVNTGLKA
                     ALTNYSTSLVAVQGESSSSGSSQTLTVTGDVNQTVTSGNAISNIVFTWGGEATDASVT
                     GLPASGIDYIKDAAAKTITISGTPTATVSYTVTTSGQSGTPVSISGTIIVTSGSGSSG
                     AMIHNFTTDGKTSSFYSITGNLSTSYGTVTYQGLTLTQCLKMESSTNISFTTTSEATL
                     TLVFNPDYTGTVKIDGTTYTPSSTNGIVTISSLPAGSHTIVKGSGSSYLFYMSTVYNS
                     ISGISNTNQSTSIQLYPNPVSNLLNINSNEKVTKIEIFSISGQIITQLNGNSSSIDLS
                     NLSSGNYLIKIHTQNGVLTKLIVKK"
BASE COUNT         2689 a         1937 c         1616 g         2853 t
ORIGIN      
        1 atcaatatca agaataaacc ttattacctg tcaacttttt tatataatta aaacgtgtaa
       61 tcgttatttc gaaatcatgt ttaatttcat cagaatataa tttatttttt aaaggtctga
      121 tggaacccac atgtctgaat attatattca ttcgtgtttg tgtatgcaat acatgtgggt
      181 ttcaaataaa tgttctgtaa gacttttcgg tactgaaaat taaatgcaaa aggcatcaaa
      241 tccgccgtca acaatggcaa cagtgcccgt aacaaattta gaagcatcgc ttactaaata
      301 atgcatggtt cctaacaaat cttccggaac gccaaaccga cggaaaggtg tatgagcaat
      361 aacggctttt ccacgatcgg tataagaacc atcaggatta atcaataaag tgcggttttg
      421 ctccgtaata aaaaatccgg gacaaatagc attgacgcgg aagttttcac caaacttggt
      481 agccagttcg cctgccatgt attttgtaaa gttggttacg gctgctttgg ctgctccgta
      541 tccggctacg cgagtcaatg gacgaagtgc ggaagccgac gaaaagttga tgatatttcc
      601 tctgttggct ttgaccatac attcggcaaa aacaattgtc ggaatgacag aacccatcag
      661 attcaaatct accactttac gaaagtcatc gagtttcagg tcgaaaatgg tgttgttcgg
      721 tccaatggta gcaccgggca tgttgccacc ggcagcattg atcaacacat ccacttttcc
      781 gtacttcgca aggatatctt cgacattttg tcttaaaatg gtttcattgg taacatcagt
      841 taccagaaac atagcttcgc caccctttcc cttcacttct tcgacgagtt tgtcgccttt
      901 ttccttgctt cgagccagaa cagccatttt tgctccgtgt gcagccaaat atttcaccat
      961 cgattcaccc agaacgcctg ttcctccggt aaatgcgata actttatcct taatgtcaaa
     1021 aagttcgtac atgatattta tttttagaat tatgtttcaa aattataaaa ttaattcatt
     1081 ctttctgaga gagttttcat aaatcgaaca atcttttcaa atgtcgatcg taaatatttt
     1141 cctctacaca ctggccaata atctcttcat atttttcgag caatgaaata tattcatctc
     1201 ccatttcggc agcaaccttg taagctacat gaatcaactg tcggaaattg ggattataat
     1261 cggggcaagt ttggtcatga cgcaacgtgt ttacgaattt ttcacttccc catgcgttaa
     1321 cttcttccac tgacggcagt ttacttccat caatatcaat cacatcagca taaggtgcgc
     1381 ataattcatc ttttcggtta taagagttgg cgtagatttt tttagccgcc atcagtccat
     1441 ctcctccggc tttagccaat ccaataactt cctccaacca tgttgttccg gctgttttaa
     1501 ggtgtaagcc tttgtcgtgt tttttaatga ttcggctcat aacgggataa atggaaaact
     1561 tgtcgctgcc cgaatgaacg cttaatttca actcttccgg aagtccaaat tctttcacag
     1621 caaaatcgat aaccaacaca tcttcttcaa attctttagc aaattgttcc aaatcgcctg
     1681 cataatcgac tcctttgtta aatcggccgg taaatttggg cgctatggtt tgaaccggta
     1741 cacctttgtc ggccaacatt ttaagtataa aaaacaattc aacaggcgtt tgaggagact
     1801 ccacttcatc catcgaaact tccgtaataa aattatcggc tcccttttct tttttgagat
     1861 aggcataaat ttcagaagct tgttgcgtag cagccaaaaa tttatttgtt attttttcca
     1921 acagatcctt tgatacattc aatggttcct ttaatccggg aatttcaagt tgccccatgt
     1981 actttttgca ggaatcaata aattgattca cctcttcctt tgtgctttct tttccgatat
     2041 acgcagccac atcgagtgta aagaaatcgg aaaccggtac aaaaggagca accgtattca
     2101 aattaatatg gtcggcatca acaaaatatg gccctttata atttaaagct ttaacagcat
     2161 tgtcggcttc cactctcgta tctttcggtt gagaatggac ataagtatgc tcacgattcg
     2221 atttattcca tacgggaata atatttaatc ctttttcatt ggctttcatt aaagctttca
     2281 attgcgcttt tccctggtgc gaaaatctgt ctccgacacc aaaactatat tttcctaatt
     2341 tcattatgaa tattttttaa atgaataaaa atcaaaattc aaaaaagttt tttgcattga
     2401 aataagaaat gttttccacc atttttccca gaaagtccat ttcggaagca ggcagcaaac
     2461 cgttttccac atcattgcct atcaaattgc aaagaatacg acgaaaatac tcgtgtcgcg
     2521 gataagacaa aaagcttcgt gaatcggtca acattccgac aaaacgactg agcaatccta
     2581 aattggaaag tgagttcaat tgagcttcga tgccggattt ctgatcgaga aaccaccatg
     2641 ccgcaccgaa ttgcattttt cccggaacgc taccatcctg gaaatttcct atcatggtgg
     2701 caacaagatc gttgtcgcgc ggatttagat tgtaaatgat agtttttgta agctttccgg
     2761 aaacatccaa actgtcgaaa aatgcactca tggcttttgc aaccgtaaaa tcgccaatgg
     2821 aatcgaatcc ggtatcgggc cccaactgct tcatcatcct ggaattgttg ttgcgaatag
     2881 ctccgtaatg aaattgctgt gtccaacctt tttcccaatc cataacagcg ccttcgtaaa
     2941 gcatggccga tttgaattta cgaatttctt cctgagttaa ttccttacca ccgtaaactt
     3001 tgttgaaaat gacttcaatt tccgattttg accacttttc tgcataaaat tcttcaattc
     3061 cgtgatcgga aagtttgcag cccaaatcgg caaaaaaatc atgacgaacg cgcaatgctt
     3121 gtaacaaatc atcaaatttg cgaatggtaa cgcctgaaac ttccgaaagt ttctcgacat
     3181 attttcgata ttccaccgga ttttccaccg ccattgcttt atcgggacgc caagccggca
     3241 acactttgat accgaagcct tcttgcttca aagctatgtg ataattcagg ctgtcaaccg
     3301 gatcatcggt cgtacataca acttccacat tgaatttttt cattaagccg cgtgccgaat
     3361 attccttcgt acggagctta gcggtacatt catcaaaaat ttcttttgcc gtagccgggc
     3421 ttagcaattt atccacgcca aaagcctgtt tcaattccaa atgcgtccag tgatacagtg
     3481 gattgcgcat ggtataagga acagtttcag cccatttttc aaatttttcc caatctgaag
     3541 tgtctttccc ggtacaatat ttttcgtcaa ttccgttgga gcgcatagca cgccatttgt
     3601 aatgatcacc ttcgagccag atttgtccca gattgtcgaa ctgacgatcg ttggcaatgt
     3661 attcgggatt caaatggcaa tgataatcaa taatcggttg atgttcggca tgttgatgat
     3721 aaagctcttt tgctgcatca ctttgcagta aaaaatcatc gttaataaaa ctttgcataa
     3781 tattctgttt taaaagatta ttttctgtaa aaatataaac tgtgtgcgca cacaaagtta
     3841 taaaaatatt ctcattttaa taagcatata aatttatttt cataatatta acaaatgaaa
     3901 cgatcaaaga tgaatgaata acctttaaaa tggattcaat ttcatttttt atttatactg
     3961 atcgacactt tctgattcag acagttaagc tcatcatcta tggcatttat aaacgatgct
     4021 ttatcctgaa ttggctgcca ttccttttcc cacgtagcca taaaataata ttcaacataa
     4081 ccatgatccg gcgtaagaac gataacgtga ttcagttcgt cttcggtcaa agtctgcaat
     4141 tgcctttttc tataaaaaat cgcaagtccc atattatcat tattcatgct ctgacaacca
     4201 aaagtagcat aatatgtcca ttccccgtcg ttggctggag gcacaattat ttcggtattc
     4261 ttcaacttaa taattcctgt ggctatatta tccaatttcc catcaatttt caattccatg
     4321 tgcgaagccc gatgccctgc atcaatggtt atcagtgaag ttaaattgac tttgtttgct
     4381 gcttcccaac cgtaataaac ggtttttatt tgtgaacgaa ctttcccatc cgaaatgaca
     4441 caacacacca aactgtcgcg tttttcgact ctgaaagctt ttttcccatc ccaataagca
     4501 atgctgccaa tacccagcga cttgccaact tccatatcgt ccattcccca aacggccatt
     4561 ttatgataat tgtcgtaacc atcaacgccc actgccggta aaacaattcc gggcgttttt
     4621 ttcccaaagg catccagcgc atttcgctga tcgaggtaaa atcgataggc caccttgtcg
     4681 ctttcccagc cggctccttc atattttata aaataggaat gatccttaaa atttccgggc
     4741 aaacggataa aattgggctt cacccacgaa aattcaccct gatattttcc gtttacaaac
     4801 tgacctccaa ttttatgagc aatttctgca taggttcgtt tgggataatt ttcagcttgt
     4861 ccacgctgaa agttaaattt taatatagca ttcggctgca atctttcaat cgaaaaaact
     4921 gcgatttgtt cagcatttat atcggaaaca atttcaaccg gcaattgaac gccttctttc
     4981 gaaactacat agtaatttcc aatggttaag ggcaatttat tcaccggaat ttcgaccata
     5041 aaatccttca acaagacgtt ggattgattt ttaacggtaa ccgactgtga ataaacatta
     5101 tgaataagca atgcaaaaac tattaaagat attgatctac tgattttgaa cattttactt
     5161 tatattttaa tgattttctc gatttttctc aaaaaaatct tttccgagta ctatttcctt
     5221 gtcgaaattt ttaacttgtc gggatgaaag cgtttttgac catttcactc gacgattggt
     5281 caaattacca ccttcacctg aattttgata ttccgaaaat tgagccgttt tttcattgga
     5341 aacttttccc caattgtccc acccttcggg ttttatgacg ttggtcatgt cgcattcgat
     5401 aaaagctact tttgcataag gacgccacgg tcttcccaaa taaaatgaag ccggattttc
     5461 gccatgaatt tgacaacgat aaaacacaaa tccatagttg ttctcttgag gcgttgctgc
     5521 tgcggttata aatccgcttt ctttgcagta aagttcacaa gagtcaaaat aggcagttga
     5581 aaagccaaaa atgaaatcaa ccgtcccttc gatgtaacaa tttttatagt attgcctgct
     5641 gccggctttg tgtgggtaaa gtgtgtcctg aaatccccaa aaattacaat ttaaaaaacg
     5701 tgccttatcg cttcgaacaa ttgcggcaac cgcctgacca atcggtccgg ctgcgttttc
     5761 taaagttaaa ttttcagccg taaaattggg tgaacaaata taaacacttg cagaaccgga
     5821 agttcccaat tgatcgccga aaccattcgg ttttgaagca aaattattgt acgaaagaat
     5881 cgttttattc ctgtcttctc caatcaaggt caacgaatcc ttcgtttcag gaataatgag
     5941 tttttctctg taaaatccgt tgcgaataaa aatactggtt ttccttttgc gaaaatcggg
     6001 cacggcattg atggcttcct gaactgttcg aaaatcgccc gttccatctt gagcaacgat
     6061 cagatcatat ttcgaatgat cgactgcaat tggagtattg tataaaattc gatttagaaa
     6121 attggcaaca tattgaaccg tagataattg ccacggatga aaatgccaaa aagaatgagg
     6181 acaatcatca aaagcatgta cttcggaata aatgcccaaa ctgtccaaca ttcggatatg
     6241 ttcgtctctg ccattatgaa agcgtggaat ggaactgttg ataaaacaga ctggagcaga
     6301 attctgattg acccaagtta ccgctgaagc aactttccaa ttttcgggac gttcggcaaa
     6361 tgttccgccc agccacaagg catcaaccgg cattttatct ccatcgtttc gagctttttg
     6421 tgctctgtca atggtcgctt tgtcgataaa ggaagaaatg ccatcgatat tgacaacggc
     6481 tttgatgagc ttatctttat tttttgttcc gattaaagca gccaattgtc cccctgccga
     6541 gcatcccgaa acggcaattt tatcccgatt gattccgtaa gtttctgcgt tatccgcaat
     6601 ccatcttacg gcatcttcca aatcttcttc tccggcagga aataaagctt ccggaataag
     6661 acgatattcg acggtaatac agacaaaacc tttttgagcc agtttcactg ccattgcttt
     6721 ttgtaatgaa ggagaacctg aattccaacc tccgccatga accatgatta cggcaggtaa
     6781 aacttcattg ttatccaacc gataaatatt caacaacagc ttcctttccg gtgaaatgga
     6841 tttgtaagca atgttctcgt acgatttcag attgtcggac attcttgcct ccgccgggcg
     6901 aataaaagga tactgttttt tcaacttgat ataagtactg tagaccgtgt agcttgtatc
     6961 tcgcggaaca ccgtttatca ttactgtttg tgcctttacg ggaaagcata aaacaatcgc
     7021 accaaaaaga aatattgaat gagaaaattt catgataaaa agttaaaata caatacaaag
     7081 ggtgaaatag cagtgaatga atatggtctt gaataaactt cactgctatt tgcccaaaat
     7141 aagaattatt tcttcacgat cagcttggtt aggactccat tttgcgtatg aatttttatt
     7201 aaataattgc cacttgacaa attgctcaaa tcaatactcg agctgtttcc atttaattgc
     7261 gtaattatct gacctgaaat actaaagatt tcgattttcg ttactttttc atttgaattg
     7321 atgttcaata aattggaaac aggatttgga taaagctgga tagaagttga ctgatttgtg
     7381 ttactaattc ctgaaattga attgtaaaca gtactcatat agaacagata actggagccg
     7441 cttcctttta caatcgtatg agatccggca ggaagtgagc taatggttac aattccattt
     7501 gtagaggacg gcgtgtaagt agttccgtca attttaacgg taccggtata atcaggattg
     7561 aataccagcg ttaaagtagc ttccgaagta gttgtaaagc tgatattggt actcgactcc
     7621 attttgaggc actgtgttaa ggttaagcct tgataagtaa ccgttccgta acttgtggac
     7681 agattgcctg taatagaata gaaagaactg gttttaccat ccgttgtaaa gttatgaatc
     7741 attgctcccg aagaacccga tccgcttgta acgataattg tgcctgatat tgaaaccgga
     7801 gttcctgatt gtccgcttgt ggttaccgta taggaaacag tagctgttgg tgtaccggaa
     7861 atggtaatcg ttttggcagc agcatctttt atgtagtcga ttcctgaagc cggcaatcca
     7921 gtaactgaag catctgtagc ctcaccaccc caagtgaaaa caatattact aatggcattt
     7981 cccgatgtta ccgtttgatt aacatcacct gttacagtca atgtttgcga gctgccggaa
     8041 gaactacttt caccctgaac ggctactaaa cttgtactat aatttgtaag agccgctttc
     8101 aaacccgtat ttacatcata agatgcatca tcaacagcat tgttaaatgt ccatttcaaa
     8161 tcgccaccat taactcgtcc ggcgtattgc attacttttt cttttgcttg ttctggggta
     8221 tctgctgtat attggtacat gacagacgca tccgtatcga aattattata ggtatttgag
     8281 ccttgttttg tcttcactgt cgaaggaaca gtttcacttc ttgaagcaac tacataagca
     8341 tcgaaatcga cagttgaatt ggcaaaatcg gagcttccat aagcaacaaa acgattttga
     8401 ccggtcatgt aattgttaaa tgctttgata ataccgccat cttctcctga aaaagtacca
     8461 cttgactgat agatatcagt accttgcttg gaaatcagca tgggatattt gcaattgcgg
     8521 aaataatttc cttctacaaa tacagaggca ccggtggttg agccaacacc gtattttgca
     8581 tttccatcgt aataattatt gtaaacatgg gtagaataat agcgaacgcg aggatgacgc
     8641 gaatcggaat gatcgtacca gttgtgatga taagtaatga aaagaccgga tgttgttcct
     8701 tcacttaaac caagcaaatt gcattttcca caatcccaga aatggttgta agaaaaagta
     8761 acataagtag attttttaca gtcaagtgct ccgtcacctt taatttgatc ggcatcgctg
     8821 ccggctgcgc catagaaaaa gtcgcaatta tgcacccaga tgtaatcatt attttgttgt
     8881 aagctgacat catctccttc ctcactgtcg caattcataa aaccaagatt tctgacttcg
     8941 atgttagaag cattttttat acgtaatccc caaccatcgg ctactgcatc gtcgccaatt
     9001 ccttcaaaag tgatataact tgaagcatta ttgttgttct cgatcacaat atctccattc
     9061 aacatataat caaggtcggt aatttgccca atcaa
//
LOCUS       DAOTAX010000065         9005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_6577_length_9005_cov_30.686592, whole genome shotgun sequence.
ACCESSION   DAOTAX010000065 DAOTAX010000000
VERSION     DAOTAX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6577_length_9005_cov_30.686592"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..513)
                     /locus_tag="PLM04_07030"
     CDS             complement(22..513)
                     /locus_tag="PLM04_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02862.1"
                     /translation="MEKGTKSYISGNQLEVAVKTVLIGKGFELVKYRDWEKNQEKYGK
                     ELLLENVPFTTIYEHNGNTEFLLISEKYELKIRIECKWQQVGGSVDEKLPYLYLNTIE
                     AMLEETIMILIDGSGWKTGAIKWLKDAVKQKKYTSEENKHKEILVFSLADFFTWANKT
                     FNR"
     gene            complement(513..1502)
                     /locus_tag="PLM04_07035"
     CDS             complement(513..1502)
                     /locus_tag="PLM04_07035"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HON02863.1"
                     /translation="MAIDTKYLTSDTIGSVVQDLFNPSNVGAKPFLKWAGGKGQLLDI
                     FQKLYPEQLKRKKIKNFYEPFLGSGAVFFDITQKYEIENAFLYDINEELILAYKVIQR
                     DVSKLIDFLYRYQKTYLKLDKVKRKEFFYDQRTNYNLQRFNIDYDKYSENWVPRAAQL
                     IFLNRTCFNGLYRVNLKGEFNSPVGDYDNPTICDEQNLLAVNKVLEIAEIKKADFKDI
                     VTDLRSDSFVYFDPPYRPISKTASFKAYHKQGFTDSEQLQLAQLFKQLDSEGSYVMLS
                     NSDPKNIDPDDNFFDDMYNGFNIVRVPARRMINSDPTKRGRINEIVVTNYTTA"
     gene            complement(1628..2515)
                     /gene="dapA"
                     /locus_tag="PLM04_07040"
     CDS             complement(1628..2515)
                     /gene="dapA"
                     /locus_tag="PLM04_07040"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HON02864.1"
                     /translation="MTINNKLTGMGVALVTPFKSDDSIDFDALGRLVEYIINNNADYL
                     VVCGTTAETPTLTDKEKDEIARFVVQCNAGRLPIVLGIGGNNTRQVVEKVKTSDLSGI
                     DAILSVTPYYNKPSQEGLYQHYSAIAKASPLPLVLYNVPGRTGVNMTAETTLRLAKEF
                     SNICAVKEASGNFTQIDDIIKNKPQDFMVISGDDGITFPLITLGAVGVISVIGNAFPR
                     EFSRMVRLALQGDYQNARQIHYRFTELFELLFVEGNPAGVKSMLAVMGMIENKLRLPL
                     VPNTIKTYEKIRSVLNQLN"
     gene            complement(2546..3124)
                     /locus_tag="PLM04_07045"
     CDS             complement(2546..3124)
                     /locus_tag="PLM04_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02865.1"
                     /translation="MKFLSRKKIKNAAIKLSDANFHIPKFLNYSDIHSILLLFEADDA
                     EKNLQVKKIVQMLKADGKKVTAIGCPRNKKLKTDFPIFENLTIVDKSQTDFFEKPRHS
                     VFKPIEANKFDALLDLSLNENIPLQYIALYSNALLKIGVRKTSFPLYDFILDIDSIVA
                     NLKTDHLPIDLLYIFNQIIFYLKNIQSCDEKA"
     gene            complement(3198..5219)
                     /gene="ligA"
                     /locus_tag="PLM04_07050"
     CDS             complement(3198..5219)
                     /gene="ligA"
                     /locus_tag="PLM04_07050"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HON02866.1"
                     /translation="MTVKEQIEALRQEIEQHNYYYYVLSQPIISDYEYDMKMKQLQEL
                     EEKYPEYFDPLSPTQRVGSDITTSFEQAYHTYPMLSLGNTYTEGEVKDFYERVRKALN
                     EPFELVCELKYDGVSISITYEHGEIQRAVTRGDGEKGDVVTANIKTIRSIPLRLKGDY
                     PDKFEIRGEVLMPWAVFEALNKEREEQEEPLFANPRNAAAGTIKLQNSAIVASRKLDA
                     YFYYVLGEQLPFDTHFENLQAARGWGFKISENIKLCRTLDEVWEFIHFWDKERKNLPV
                     PIDGIVLKVNSLEQQRKLGTTAKSPRWAIAYKYQAEKALTKLLSVTFQVGRTGTVTPV
                     ANLEPVQLSGTTVKRASLHNADIIESLDLHIGDMVYVEKGGEIIPKITGVEKSARPQN
                     GEKVVFIKNCPECNAPLIRYPGEAAHYCPNENGCPPQIKGKIEHFVSRKAMNIEGLGE
                     ETINLLYENGLLHNVADIYDLKISDLVSLERLGLKSASNLISSIEKSKEVPFERVLFA
                     LGIRFVGETVAKKLAYAFKSIEALQQADYEDLIQVEEIGDKIAQSVRQYFSDPKNLEI
                     INRLKAAGVQLSLNDDKSKRKGNELEGKSVVISGTFKYHSREEYKDLIEAHGGKNVGS
                     VSSKTSFILAGENMGPEKLKKAQNLGIELIDENKFLSMINEEIPSSATE"
     gene            complement(5344..>6201)
                     /locus_tag="PLM04_07055"
     CDS             complement(5344..>6201)
                     /locus_tag="PLM04_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HON02867.1"
                     /translation="MKKLGILFSAIILLICSGFVSAQQKKTITLNVATFNLRLDTEDD
                     GENAWPNRKEFVKSLIRFHDFDIFGTQEGYKHQLDDILKLGGYAYVGVGRDDGVDEGE
                     HSAIFFKTNRFEVLDKGNFWYSKTPEVPSKGWDAFCCNRLCSWGKFKDKSSGKIFFVF
                     NSHFDHIGHIARKNSSLLLLKRIGEIAGNFPVFCTGDFNSTPDAEPIQIIAKSGKLFD
                     SYHITKQPPYGTVGTYNSFSIKSKMDDRIDYIWVTSGISINKYGVLNEQQYGRFASDH
                     FPVMVSATL"
     assembly_gap    6202..6301
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6409..6954
                     /locus_tag="PLM04_07060"
     CDS             6409..6954
                     /locus_tag="PLM04_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HON02868.1"
                     /translation="MFIAQRLKKENICEYLLYMWQIEDLIRAFQLNIDLIDENIIAPY
                     PISETEKRTMYEWYESLIDMMRKENVQEKGHLQLNKNVIIDLNDFHELLLQSGADSEY
                     NRLFYSILPHLVQLRKKAEVNINDIELCFNFLYGIMMLNLKKEKISDETAAAQNDISK
                     FMALLAKDYKLYQEGELDLYK"
     gene            7033..7905
                     /gene="rfbA"
                     /locus_tag="PLM04_07065"
     CDS             7033..7905
                     /gene="rfbA"
                     /locus_tag="PLM04_07065"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HON02869.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPLSILMLA
                     GIREILIISTPQDIHLYERLLGDGSDFGIAIEYAVQPSPDGLAQAFIIGENFIGNDNV
                     CMILGDNIFYGFDFSRTLIEAANLKDGAIVFGYYVNDPERYGVAEFDAEGKVLSLEEK
                     PKQPKSNYAVTGLYFYSNDVVEKSKHLKPSSRGELEITDLNRLYLNEGRLNMKILGRG
                     MAWLDTGTHDSLLDASNFIATIENRQGLKIACLEEIAYRNGYIDREQLLRLAEPLKKS
                     HYGEYLIKIAGEVK"
     gene            8322..8558
                     /gene="rpmB"
                     /locus_tag="PLM04_07070"
     CDS             8322..8558
                     /gene="rpmB"
                     /locus_tag="PLM04_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HON02870.1"
                     /translation="MPKICQITGKKPLKGNNVSHSKRRTKRNFDVNLFTKKFYWVEED
                     TWISLDISAAGLRTINKLGLDASIKKALKKGYLK"
     gene            8581..8769
                     /gene="rpmG"
                     /locus_tag="PLM04_07075"
     CDS             8581..8769
                     /gene="rpmG"
                     /locus_tag="PLM04_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HON02871.1"
                     /translation="MAKKTKEARVQVILECTEHKDSGMPGTSRYVTQKNRKNTPERLE
                     LRKYNPILKRVTIHREIK"
     gene            8774..8926
                     /locus_tag="PLM04_07080"
     CDS             8774..8926
                     /locus_tag="PLM04_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HON02872.1"
                     /translation="MAKKVVASVQKGEGRTFSKVIKMVKSPKTGAYMFTEEMVPNDKV
                     KDFLNK"
BASE COUNT         2832 a         1648 c         1551 g         2874 t
ORIGIN      
        1 ggggggtgtg ttcgtgcttt tttatctgtt aaatgttttg tttgcccaag taaaaaaatc
       61 ggccaaacta aaaactaaaa tttctttatg tttattctct tcacttgtat attttttctg
      121 tttaacggca tcctttaacc atttaattgc gccagttttc caaccagagc catctattaa
      181 tatcataatt gtttcttcta gcattgcttc aattgtgttt aaatacaggt acgggagttt
      241 ctcatcaact gaaccaccaa cctgttgcca tttgcattct atacgaattt ttaattcata
      301 tttttcagaa ataagcagaa attctgtatt tccgttgtgc tcataaattg ttgtaaaagg
      361 aacattttca agtaataatt ccttaccata tttttcttga tttttctccc aatctctata
      421 ttttacaagc tcaaatccct ttcctatcaa aaccgtttta acggcaactt caagttgatt
      481 gcctgatata taacttttag ttcctttttc cattatgctg ttgtgtaatt tgttaccact
      541 atttcattta ttctgcccct tttggttggg tccgaattta tcattcttct tgcaggaact
      601 cttactatat tgaatccatt atacatgtca tcgaaaaaat tatcatcagg atcaatattt
      661 ttagggtctg aattactcaa catgacataa gatccttctg aatcaagttg tttaaaaagc
      721 tgtgctaact gcaattgttc actatcagta aaaccttgtt tgtgatatgc tttaaaactt
      781 gcagttttac ttatcggcct ataaggtgga tcgaaataaa caaaagaatc agatcttaag
      841 tctgttacaa tatctttaaa gtcagctttt tttatttcgg caatttctaa aaccttattg
      901 actgccaata aattttgttc atcacaaatt gtcgggttat cataatcgcc taccggtgag
      961 ttaaattctc cttttaaatt caccctgtaa agtccgttaa aacatgttct gtttaaaaaa
     1021 attagttgtg cagcacgagg aacccaattt tcagaatatt tatcataatc aatattaaac
     1081 ctttgtaaat tataatttgt acgttgatcg taaaaaaatt ctttacgttt tactttatca
     1141 agtttcaaat aagttttttg gtaacgatat aaaaaatcaa ttagtttgct aacatccctt
     1201 tgaattactt tatatgccag aattagttct tcattaatat cataaagaaa agcattttca
     1261 atttcatatt tttgagttat atcaaaaaaa actgctccac ttcctaaaaa tggctcataa
     1321 aaattcttga tcttttttct cttaagttgt tcaggataaa gtttctgaaa aatatccaaa
     1381 agttgtcctt tgccaccagc ccactttaaa aatggttttg cgccaacatt agatgggtta
     1441 aataaatctt gcacaacaga accaattgtg tcagaagtta aatattttgt atcaattgcc
     1501 ataattctat ttgaatgtcc aaatttacaa aaatattttt tgtgttactt cattctaatg
     1561 atgtagcatt ttttagcatg acaactttcg tcctgtcggg gaaaattttt tgtaattttg
     1621 taatttttca atttaactga ttgagtaccg agcgaatttt ttcgtacgtt ttaatggtat
     1681 tgggcaccag aggaagacgc aatttgtttt ctatcattcc catgacagcc aacatgcttt
     1741 ttacgccggc aggatttcct tccacaaaaa gcaattcaaa aagttcggtg aaacgataat
     1801 gtatctgacg tgcattttga taatcgccct gcagagccaa acgaaccata cggctgaatt
     1861 cgcgcggaaa agcattgccg ataacggaaa tcacaccgac agcacccaac gtaatcaacg
     1921 gaaaagtaat gccgtcgtcg ccggaaataa ccatgaaatc ctgaggcttg tttttgataa
     1981 tatcgtcgat ctgggtaaaa tttccggagg cttccttcac tgcgcaaata ttggaaaatt
     2041 cttttgccaa gcgtaaagta gtttcagcag tcatgttcac gcctgttctt cccggcacat
     2101 tgtagagcac aagtggcaac ggagaagctt tggctatggc cgaataatgt tgataaagtc
     2161 cttcctgaga aggtttgttg taatacggcg taaccgataa aatggcatca atccccgata
     2221 aatcggaagt tttcactttt tcgaccactt gtctggtatt gtttccgcca attcccaata
     2281 caataggcaa tcttccggca ttgcactgaa caacaaaacg ggctatttca tccttttcct
     2341 tgtctgtcaa ggtaggtgtt tcggctgtag tgccacatac aaccaggtaa tcggcattgt
     2401 tgttgattat gtattcaacc aaccttccca acgcatcaaa atctatcgaa tcgtcgcttt
     2461 taaaaggtgt tactaaagct acacccatcc ctgtgagctt gttgttaatc gtcatggagt
     2521 gaaaaaattt tattctgtta caaaattacg ctttttcatc gcaactttgt atatttttca
     2581 ggtaaaaaat tatctgatta aaaatatata acaaatcgat gggcaaatgg tcagtcttta
     2641 agtttgcaac aatgctgtcg atatccaata taaaatcata caatggaaac gatgtttttc
     2701 taactccaat tttcaacaat gcattggaat acaaagcaat atattgaaga ggaatatttt
     2761 cgttcaagga taaatccagc aaagcatcaa atttgttggc ttcgattggt ttaaagacag
     2821 aatgtctggg tttttcaaaa aaatcggtct gacttttatc cacaatcgtt aaattttcaa
     2881 aaatcgggaa atcggttttc aatttcttat ttcgaggaca accaatagct gtaactttct
     2941 tcccatctgc tttcagcatc tgcactattt ttttcacctg aagatttttt tccgcatcat
     3001 cggcttcaaa caacagcaaa atagaatgaa tgtctgaata atttagaaat ttgggtatat
     3061 gaaaattggc atcggacaat ttgatagctg catttttaat tttttttctg ctcaaaaatt
     3121 tcattgtaat ctctttaaat aggttgtttt gggttgtcct gaaaaaacac agaaattgaa
     3181 ctgttttata attttattca ttcagtggct gatgatggaa tttcttcatt tatcatcgaa
     3241 agaaatttat tttcatcaat tagttcaatg cccaaatttt gtgccttttt cagtttttcc
     3301 ggtcccatgt tttcacctgc cagaataaaa gaagtttttg aagacacgct gccaacattt
     3361 tttccgccat gtgcctcgat taaatctttg tattcctcac gtgaatggta tttgaatgtt
     3421 ccgctgatga cgactgattt tccttcgagc tcgtttccct tgcgtttgga tttatcatca
     3481 ttaagagaca gttgaactcc ggcagctttt aagcggttaa taatttccag atttttcgga
     3541 tcggaaaaat attgtcggac actttgagct attttatctc caatttcctc cacctgaatc
     3601 aaatcttcat aatcggcttg ttgcaaagct tcaattgatt taaaggcata agccagcttt
     3661 ttggcaaccg tttcgccaac aaaacgaatt cccagcgcaa acaatacgcg ttcgaaagga
     3721 acttccttcg atttttctat tgaactgatc agattagaag ccgatttcaa acccaatcgt
     3781 tccaatgaaa ccaaatcgga aattttcaga tcgtaaatat cggccacatt atgaagcaaa
     3841 ccgttttcgt aaagcaaatt aatagtttct tcacctaaac cttcgatgtt catggctttt
     3901 ctgctcacaa aatgttcaat ttttccctta atctgaggcg ggcaaccgtt ttcgttggga
     3961 caatagtggg cagcttcgcc gggatatcga atgagaggag cattgcattc gggacaattt
     4021 tttataaaaa caactttttc gccgttttgt ggccgtgcgc ttttttccac accggtaatt
     4081 ttcggaataa tttctccacc tttttcgaca taaaccatat cgccaatatg caaatcgagc
     4141 gattcaataa tatctgcatt atgcaacgaa gcgcgtttca ctgtagttcc cgaaagttga
     4201 accggttcaa gattggctac gggtgttacg gtgcctgttc ttccaacctg aaacgtaacc
     4261 gaaagaagtt tggtcaatgc tttttcggcc tggtatttat aagcaatggc ccaacgtggc
     4321 gattttgcag ttgtgccaag ttttcgttgt tgttccaatg aattgacttt caatacaatg
     4381 ccatcaatcg gaaccggtaa attttttcgt tctttgtccc aaaaatgaat aaattcccac
     4441 acttcgtcga gtgttctgca aagtttaata ttttcagaaa ttttaaaacc ccaaccacga
     4501 gctgcctgca aattttcaaa gtgcgtgtca aaaggaagtt gttcgcccaa tacataataa
     4561 aaataggcat ccagcttacg ggaagcaacg atggcagaat tttgaagttt aatggttccc
     4621 gcagcagcgt tgcgaggatt ggcaaaaagc ggttcttcct gttcttctct ttccttattg
     4681 agtgcttcaa aaactgccca cggcatcaac acttcgccgc gaatttcaaa tttatcggga
     4741 taatcgcctt tcaatcgcaa gggaatgctc cggatagttt tgatgttggc tgttactaca
     4801 tcaccttttt ctccgtctcc acgcgttaca gcccgttgaa tttcaccatg ttcgtatgta
     4861 atggaaatgg aaaccccatc atatttcagt tcacatacca gttcaaaagg ttcatttaat
     4921 gctttccgaa cacgttcata aaaatctttc acttcacctt ctgtataagt atttcccaac
     4981 gaaagcatgg gataagtatg ataagcctgc tcaaaactgg ttgtaatatc gctgccaaca
     5041 cgctgggtgg gcgaaagagg atcaaaatat tcaggatatt tttcttccaa ttcctgcaac
     5101 tgcttcattt tcatatcata ttcgtaatcg gaaataatgg gttgcgaaag cacgtaataa
     5161 taatagttgt gctgctctat ttcctggcgc agcgcttcga tttgttcctt gactgtcata
     5221 aattagaaaa ataataattc ctgatgggca aaattaatca attaaagaaa agtgaacaag
     5281 aaaagggcgg aaattggaag aaagtcgcaa aattcccgcc ctcaaaaaaa taaaattaaa
     5341 atttcaaaga gtagcgctga ccattaccgg gaaatgatcg gaagcaaagc gcccatattg
     5401 ctgttcgtta agtaccccat atttattgat ggaaataccg ctcgtaaccc aaatataatc
     5461 aattcgatcg tccattttgg attttattga aaatgaatta tatgtaccca ccgttccata
     5521 aggaggttgt ttggtaatat gataactgtc gaaaagtttt ccgcttttag caataatttg
     5581 aatcggctcg gcatcgggtg tagaattaaa atcgcccgta caaaaaaccg gaaagtttcc
     5641 ggctatttca ccaatacgct tcaaaagcag taaggatgaa ttttttcgtg ctatgtgccc
     5701 aatatgatcg aaatgagaat taaaaacaaa aaatattttt cctgaagatt tgtctttaaa
     5761 ttttccccac gaacaaagac gattgcaaca gaaggcatcc caaccttttg aaggaacttc
     5821 aggcgttttg gaataccaaa agtttccctt atccaatact tcaaagcgat ttgttttgaa
     5881 aaaaatagca gaatgttctc cttcatcaac gccatcgtct cgtcctacgc caacataagc
     5941 atatccaccc aattttaaaa tgtcgtccaa ttgatgttta taaccttcct gtgttccaaa
     6001 aatgtcgaag tcgtggaaac gaatcagact tttgacaaat tctttccgat tgggccatgc
     6061 gttttcacca tcatcttccg tatcgagccg aagattaaaa gttgcaacgt tgagcgtaat
     6121 ggtttttttc tgctgtgccg atacaaagcc ggagcaaatt aaaagaataa ttgcagaaaa
     6181 cagaatgccg agttttttca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 naaaattccc gatatttata ttaattttgc aaaatagttc agtaagagac acaaattcct
     6361 attcttaaaa atattggcat tatattcgta ccatcaaaat ctttcatcat gttcatagct
     6421 caaagattga aaaaggaaaa tatctgcgaa tacttgcttt acatgtggca aatcgaagac
     6481 ctgattcggg cttttcagct gaacatcgac ttaatagatg aaaatataat tgctccgtac
     6541 ccgatttctg aaactgaaaa aagaaccatg tacgaatggt atgaaagtct gatcgacatg
     6601 atgcgcaaag aaaacgttca ggaaaaaggt catttgcaat tgaataaaaa tgtaattatt
     6661 gacctgaacg atttccatga acttttgttg caatcgggcg ccgattcgga atacaatcgt
     6721 ttattttaca gtattttacc tcatcttgtt cagttgagaa aaaaagctga agtaaacata
     6781 aacgacatag agttatgttt taattttttg tatggtatca tgatgttgaa tctgaaaaag
     6841 gaaaaaattt cagatgaaac ggccgctgcg caaaacgata tttcaaagtt catggcattg
     6901 ctggcaaaag attataagct ttatcaggaa ggagaactgg atttgtacaa ataaaaaact
     6961 tttgagaatt cgcttacatt tttatcgttt aaaataattt cgcagaaaaa acaatttaaa
     7021 aaaatcttca tcatgaaagg cattgttttg gcaggtggtt ccggtactcg actctatccc
     7081 ataaccaaaa gcatttccaa acaaattatt ccggtttacg acaaacccat gatttattat
     7141 ccgctctcaa tacttatgtt ggcgggcatt cgtgaaattc tcattatttc cacaccgcag
     7201 gatattcatc tttacgaacg tctgttgggt gatggttctg atttcggtat tgcaattgaa
     7261 tatgcagtgc agccatcgcc ggatgggttg gctcaggcct ttattattgg tgaaaatttc
     7321 attggaaacg acaatgtttg catgattctt ggcgacaata ttttttatgg attcgacttt
     7381 tccagaacat tgatcgaagc ggccaacctg aaagacggag ccattgtttt cggttattat
     7441 gtcaatgatc cggaacgata tggcgtagcc gaatttgatg ctgaaggcaa ggtattgagt
     7501 ttggaagaaa aaccaaaaca accaaaatca aattatgccg ttacaggact ttatttctat
     7561 agtaatgatg tagttgaaaa atcgaaacac ctgaaacctt catcgcgtgg agaattggaa
     7621 attaccgatt taaacagact ttacctgaac gaaggacgat tgaatatgaa aattttgggt
     7681 agaggaatgg cctggctcga tacaggaaca catgacagtt tgctggatgc ttccaacttt
     7741 attgccacca tagaaaaccg tcaggggctg aaaatagcct gcctcgaaga aattgcctat
     7801 cgcaacgggt atatcgaccg tgaacaactg cttcgactgg ccgaaccatt gaaaaaaagt
     7861 cattatggcg aatatttgat taaaattgca ggtgaagtca aatagtttga ttctaaaact
     7921 atttttggct acaaaagtga gagagtctct tttattacgc ataagacgct tttaaatata
     7981 ttttcaaaac ttaaatgata cacttttctt gaatgttttt atcctttgaa aatataattt
     8041 gtttactggt tttatacata actgaaactt gcataaacct gtcgctttcc tgaaagatat
     8101 tttattgatt tactatggtt tatgtaaatt tttcagaccc gattaaaaat ggatttttct
     8161 gtaaagaaaa agcaattaac aaattgatta acaaacattt taatgagttt ataaagataa
     8221 atttcaaaaa atagggatac aattgcttgt atatgtaaaa aataaaatgt aattttgcaa
     8281 ctcaattttg agcagaagta taacaacata aatgaaataa aatgcctaag atttgtcaga
     8341 ttacaggtaa aaaaccttta aaaggaaata atgtttcaca ctcgaaaaga cggacaaaaa
     8401 gaaatttcga tgtaaactta tttaccaaaa agttttattg ggtggaagag gacacatgga
     8461 ttagtcttga tatttcggct gccggtttaa gaaccatcaa caagttggga ttggacgctt
     8521 ccattaaaaa agcgctgaaa aaaggatatt taaaataaat actattagga ggcatacatc
     8581 atggcaaaaa aaacgaaaga agcaagggtg caagttattc tggagtgtac cgaacataag
     8641 gacagtggta tgcccggaac ttcgcgctat gttactcaaa agaacagaaa aaatactccc
     8701 gaaaggcttg agctcaggaa atacaatcca atattaaaaa gagttactat tcacagagaa
     8761 ataaaataag attatggcaa agaaggttgt tgcatcagta caaaaaggtg aaggtcgtac
     8821 tttttcaaaa gtgataaaaa tggtcaaatc acctaaaaca ggggcttaca tgtttactga
     8881 agaaatggtg cccaatgata aagtaaaaga ttttctcaat aaatgaaaaa ataaattatt
     8941 tcctacctac aaagagggtg tcccaaaagg acactttctt ttttatatta agcagcccgt
     9001 ttaag
//
LOCUS       DAOTAX010000066         8646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_6951_length_8646_cov_32.193924, whole genome shotgun sequence.
ACCESSION   DAOTAX010000066 DAOTAX010000000
VERSION     DAOTAX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_8646_cov_32.193924"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..752)
                     /locus_tag="PLM04_07085"
     CDS             complement(48..752)
                     /locus_tag="PLM04_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HON02873.1"
                     /translation="MKTLYLIPTPLGNTSFDRIFPPVNLEIIRNLRYFIVEDIRTARR
                     FLKQVDVEIDIDKLFFYTLNEHTSPTEVDGFLQPLREGEDMGLLSEAGCPAVADPGAD
                     MVAIAQKNAFRVVPLVGPSSILLALMASGFNGQNFAFVGYLPVSPAERIKAIRKLESR
                     AYSENQTQIFIETPYRNLKLFEQILQNCRPSTLLCIAANVTLPDESIVTRSVAEWKTI
                     TPDIDKKPCIFLIYKG"
     gene            complement(820..2769)
                     /locus_tag="PLM04_07090"
     CDS             complement(820..2769)
                     /locus_tag="PLM04_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HON02874.1"
                     /translation="MISVEQLCVEFGGTTLFDNITFLIHPKEKIALVGKNGAGKTTLL
                     RILAGKQQPTRGNISYQKDITIGYLPQHMIHSEGTTLWQETEKAFGNIVQLRQQLEDI
                     QQQLVERTDYESSAYHSLLDKLSQLTEYLQISGNGNYQAEMEKTLLGLGFLRSDFYRQ
                     CSEFSGGWRMRIELAKILLSHPDVLLLDEPTNHLDIESIQWLENFLFTSGSSLLLVSH
                     DKAFIDAVTTRTLEISLGKLYDYDVNYSKYIQLRKERHEQQLRAYENQQKLIQDTEDF
                     IERFRYKATKAVQVQSRIKQLEKLQRIEVDLEDNANMHLKFPPAIHSGKIPLEIEHLS
                     KAYREHLVLDDVNLMMHRGEKVAFVGKNGEGKSTLVKCIMNEIEYSGTLKLGYNVKIG
                     YFAQNQASLLDENLTVFETIDRVAVGEIRTKIRDILGAFMFGGENSEKKVKVLSGGER
                     SRLAMIKLLLEPVNFLILDEPTNHLDIPSKEVLKEAIKAFDGTAIIVSHDREFLDGLV
                     TKVYEFGNKKVKEHLGGIYDFLQAKKMESLHELERNVSSEVKNEGEAKPESENKLNYE
                     SRKELNRKIRKLEKDVEATEGLITDLDNEIQQMNTDLQIPEKASDPEFVSTLQKKRAE
                     LERKMYEWEILSEELEKIRKEWQGQ"
     gene            complement(2821..3900)
                     /locus_tag="PLM04_07095"
     CDS             complement(2821..3900)
                     /locus_tag="PLM04_07095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HON02875.1"
                     /translation="MNYLYNFIQQNPYLLILFSFLLGKAFMPSVVRIAKKHNFVVKPN
                     KRTTHKGLIPYTGGFDIFISFIFTILIFCFPLMEKLQYIPIGIFIILMTGFIDDLLNI
                     KPTSKLLGEFVAGVFLIVFSDIRITNFFGVFGIHELPLSISYIFSFLVFLLIINSINL
                     IDGVDGLASGLGIFYNLFFGIYFHLTGNLSLAMIAYTLTGSLAVFFIYNVFSKKNKIF
                     MGDTGSLFLGFMLTFYLFEFCEMNAYQSVPQSMYMAAAPAVAICVLIVPLYDTLRVVI
                     TRLKKGVSPFKADKNHIHHLLLKIGFSHRQVTFVLLAITAFFTAFALLARQWSNLVLA
                     FIAFLLATGMIFWLWRVINRKMQRK"
     gene            complement(4037..5986)
                     /locus_tag="PLM04_07100"
     CDS             complement(4037..5986)
                     /locus_tag="PLM04_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HON02876.1"
                     /translation="MKEIFKFLNNTDTFSHLASLKYLPRWAVLFIDITISAISFLAAY
                     LVASSFIKHLPDQLLLPLFPRLILLLFTQSVFFWLFHTYSGILRYSTMTDAAKLFLAI
                     FGNCIFLSALNFVCSLYFPAPLFYYASILIYGVISFILLFGLRLTVRNIFNYFVETSA
                     QIIPVMIYGIDEAGLGIARMILSSVNSKKQYKLVGFIDGNENIVNKMVMGLRVYKHDD
                     HTIKTKVANKVNTVIVSPIKMKEINPDVDLDIFIQHHISLLTSQPMDIWEGEMPSVRQ
                     IKPLKIEDLLEREPIQISFDNIAAELKSKTVMVTGAAGSIGSEIVRQLCNFTPKLIVL
                     VDNGETAIHNLKIELQAFSTSQHFNFVLGDVRNKERMEYIMDLYRPDFVFHAAAYKHV
                     PMMEDFPCESVQVNVLGTKIMADLALKYRVSRFVMVSTDKAVNPTNVMGASKRIAEIY
                     VQSLARKMQKTGTETTKFITTRFGNVLGSNGSVVPFFQKQIEKGGPVTVTHPDIIRYF
                     MTVPEASSLVLEAGVMGEGGEIYVFDMGNPVNISHLAKKMIQMAGLIPGVDIQIAYTG
                     LRPGEKLYEELLSHKEKVRSTQHPKIMVASVAEYDFDKISVDIDNLIKYSYLCKDFLV
                     VSMMKQIVPEFKSNNSQYELLDISN"
     gene            6413..>8646
                     /locus_tag="PLM04_07105"
     CDS             6413..>8646
                     /locus_tag="PLM04_07105"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HON02877.1"
                     /translation="MTKLTKEDALLYHSQGKPGKIEVVPTKPHSSQRDLSLAYSPGVA
                     EPCLEIEKDPATVYDYTNKGNLVAVISNGTAVLGLGDIGAMAGKPVMEGKALLFKIFA
                     GIDVFDIEVNEKDPEKFIQAVKAISPTFGGINLEDIKAPECFMIEERLKAELDIPIMH
                     DDQHGTAIISGAGLINALEIAGKRMEDVKIVVNGAGAAAVSCTKLYIRLGAKPENIVM
                     LDSKGVINKQRTDLNETKKAFATDREINTLEEAVKGADVFLGLSVAGVLSIEMVRSMN
                     ENPIVFALANPNPEISYEDAISAREDIIFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     TCINEEMKLAAVYALAELAKKPVPDVVNAAYNLKRLSFGRDYIIPKPLDPRLLTIVAP
                     AVARAAMESGVARKHITDWAGYNAKLRETMGYDDKMLRSFYETARQNPKRVVFSEANH
                     LNMLKAAEMAYNEGVCFPVLLGNEEKINSIAREYNIDLSGIEIINLRHDREEARRIAY
                     AEKLAKKLGREGMTYDEAYEKMYERNYFGMMMVETGEADALITGVYTKYADAIQAAKD
                     VIGIRPGSKHIAAMHIMNTKKGTFFMADTLFNRHPSTETLIDIAKLTYRTVKFFAHEP
                     VMAMLSYSNFGSDKQGSPASVHEVVETLHREVPEMIVDGEMQVNFALNKELRDRKYPF
                     TRLAGKDVNTLIFPNLSSANSAYKLLLEMGISEAVGPIQMGLNKPVHILDVESSVRDI
                     VNMTA"
BASE COUNT         2901 a         1691 c         1558 g         2496 t
ORIGIN      
        1 aggaggaaaa tttcttatca accaaaaaaa attagattga agtgctttta acctttataa
       61 atcaggaaaa tacatggttt cttatcaata tccggagtga ttgttttcca ctctgcaaca
      121 cttcgagtaa cgatggattc atccggcaaa gtaacattgg ctgcaataca aagcaaagtg
      181 gaaggacggc aattttgcaa aatttgttca aaaagtttta aattacgata gggtgtctca
      241 ataaaaatct gcgtttgatt ttcggaatag gctcttgatt caagttttcg aatcgctttt
      301 attctttctg caggcgatac gggcaaatat ccgacaaaag caaaattctg cccgttgaaa
      361 ccggaagcca ttagcgccag caaaattgaa gaaggcccaa ctaaaggcac aacgcgaaaa
      421 gcatttttct gggcgatagc taccatatcg gcgccgggat cggctacagc agggcatccg
      481 gcttcggaga gaagtcccat gtcttcgcct tcccgtaaag gctgtaaaaa tccgtcaact
      541 tcagtgggag aagtatgttc attcagcgta taaaaaaata atttatcaat atcaatttcg
      601 acgtcgacct gttttaaaaa cctacgagca gtacgaatat cctcaacaat aaaataacgt
      661 aaatttctta taatttccag attgacagga ggaaaaattc tatcgaaaga agtattgcca
      721 agtggagtgg gaattaaata caacgttttc ataaaaatcg gctttaatac attgattgaa
      781 atttaaaaac gaattaccga caatggaacg aaaagagatt tattgtcctt gccattcttt
      841 tctaattttt tccaactctt cactcaaaat ttcccactca tacattttcc tttccaactc
      901 ggcacgcttt ttctgaagag ttgaaacaaa ttcgggatca gaagcctttt cgggaatttg
      961 caaatccgtg ttcatttgtt gaatttcatt gtccaaatct gttatcaggc cttcggttgc
     1021 ttccacatct ttttccaatt ttctaatttt tcgattcagc tctttgcgac tttcataatt
     1081 caatttgttt tcactttccg gttttgcttc accttcattt ttcacttcag aagatacatt
     1141 tctttcaagt tcatgtaaac tttccatttt ttttgcctga aggaaatcgt aaataccacc
     1201 caaatgttcc ttcacttttt tattgccgaa ctcatacact tttgttacca aaccatccag
     1261 aaattcacgg tcgtgcgaaa caataattgc cgtaccgtca aatgccttta tggcttcttt
     1321 aagaacttct ttcgatggaa tatccagatg attcgttggc tcgtccaaaa tcaaaaaatt
     1381 cacaggttcg agtaaaagct ttatcatggc taatctgctg cgttcaccac cggaaagtac
     1441 cttgactttt ttttcggaat tttcgccacc aaacataaaa gcgcccaaaa tatcccggat
     1501 ttttgtacga atttctccaa cggctacgcg gtcgatggtt tcaaaaaccg tcaaattttc
     1561 gtccagcaaa gaagcctgat tttgagcaaa atagccgatt tttacattgt atcccaattt
     1621 taaagttccc gaatattcta tctcattcat gatacacttt accaatgtac ttttcccttc
     1681 tccgtttttt ccgacaaatg ctactttctc tcctcgatgc atcatcaaat ttacatcgtc
     1741 cagcaccaaa tgttccctgt aagctttgga aaggtgttct atttcgagag gaatttttcc
     1801 ggaatgtatt gccggcggaa atttcaagtg catattggca ttgtcctcca aatccacttc
     1861 gatacgctgc aatttttcga gttgcttgat acgcgattgt acctgaacag ctttggtagc
     1921 tttgtatcgg aaacgttcaa taaaatcttc cgtatcctga attaatttct gttggttttc
     1981 gtaagctctt aattgttgtt cgtgtcgctc tttgcgcaac tgaatatatt ttgaataatt
     2041 cacatcgtaa tcatacagtt ttcccaacga aatttccaaa gtacgtgtag taaccgcatc
     2101 tataaaagct ttatcgtgag aaacaagtaa aagagagctg cccgaagtga acaaaaaatt
     2161 ttcgagccat tgaatggatt caatatccaa atgattggta ggttcatcga gtaataacac
     2221 atcgggatgg gaaagcaaaa ttttggcaag ttcaattctc atgcgccaac ctccactgaa
     2281 ttctgaacac tgtcggtaaa aatcggaacg cagaaaacca agtcccaaca aagttttttc
     2341 catttcggcc tgataattgc catttccgga aatttgaaga tattcggtca gctgagagag
     2401 tttatccaac aaggaatgat atgctgaact ttcataatct gtacgttcaa ccaattgctg
     2461 ctgaatgtct tccaattgct gtcgaagttg cacaatgttc ccaaaagcct tttccgtctc
     2521 ctgccagagg gttgttccct cactatgaat catgtgttga ggtagatagc caatggtaat
     2581 gtctttttga taagatatgt ttccgcgtgt gggttgctgt ttgcctgcca atatacgcaa
     2641 caaagtagtt ttaccagcac catttttccc taccaacgca attttttctt tgggatgaat
     2701 cagaaaagta atgttatcaa aaagtgtggt tccgccaaat tcgacacaaa gttgctcaac
     2761 tgaaatcata aatattcaaa aagtaaaaaa tagaaaatgt aagaataaac ttgcaagcag
     2821 ttattttctt tgcatttttc tgtttataac tcgccataac caaaaaatca tacccgtagc
     2881 caacaaaaag gcaataaacg ccaacaccaa gtttgaccat tgacgagcta acagtgcgaa
     2941 agccgtaaaa aaggcggtaa tagctaataa aacgaatgta acctgtcggt gagaaaatcc
     3001 aattttcagc aataaatgat gtatatgatt tttatcggcc ttaaatggag aaaccccttt
     3061 ctttaaacga gtaataacaa cccgcaaagt gtcatataac ggaacaatca gcacacaaat
     3121 agctacagcc ggagcagcag ccatatacat actttgagga acactctgat aagcattcat
     3181 ttcacagaat tcaaagagat agaaagtcag cataaatccc aaaaacaggg aaccggtatc
     3241 tcccataaaa attttgtttt tcttgctgaa tacgttgtaa ataaaaaata cggccaacga
     3301 acccgtaagc gtataagcaa tcatggccag tgacaaattg ccggtcagat gaaaataaat
     3361 gccgaaaaaa agattgtaaa aaattccgag tccggaagcc aaaccatcta ccccatcaat
     3421 taaattaatg gaattgataa tcagtaaaaa aaccaaaaaa gaaaaaatat aactaatcga
     3481 caaaggaagt tcatgaatac cgaaaacacc aaaaaaatta gttattctga tgtcggaaaa
     3541 cacaataaga aaaactcctg caacaaattc tcccaataat ttggatgtgg gtttaatatt
     3601 cagcaagtca tcaataaaac cggtcatcaa aataatgaaa ataccaatgg ggatatattg
     3661 taatttctcc atcaaaggga aacaaaaaat taaaattgta aagataaaag aaataaaaat
     3721 atcaaaaccg cccgtatagg gtatcaaacc tttatgagtt gttcgtttgt tgggttttac
     3781 cacaaaatta tgtttcttgg caattctaac aacggaaggc atgaaagctt ttcccaaaag
     3841 aaaagaaaaa agaatcagca aataaggatt ttgttgaata aaattatata aataattcat
     3901 tttgtgttgt taagtatatt tatttctctg tttctgcgaa aaaaaaaaat caataaaaaa
     3961 tcgtctgaaa ataatgaaaa aagaaattaa ttattttcaa acgcttacaa ccaaaagatc
     4021 tgcttaaact tgtgtcttaa ttactgatat ccaataattc gtattgagaa ttattacttt
     4081 tgaactccgg gacaatctgc ttcatcatgg atacaaccag aaaatccttg cagagatagc
     4141 tgtatttaat caagttatct atatcaacgg agattttatc aaaatcgtat tctgcaaccg
     4201 aagcaaccat aatttttgga tgttgggtag atctcacttt ttctttatga cttaaaagtt
     4261 cttcgtataa tttttcaccc ggacgcaggc cggtgtaagc tatctgaata tccacaccgg
     4321 gaattaatcc ggccatttga atcattttct ttgccagatg actgatattt accggatttc
     4381 ccatatcaaa tacataaatt tctccgcctt cgcccattac acccgcttcc aacaccaagc
     4441 tgcttgcttc gggaacagtc atgaaataac gaataatatc aggatgtgtt actgtaacgg
     4501 gtcctccttt ttcgatttgt ttttgaaaaa acggaaccac tgacccattc gatcccaata
     4561 cgtttccaaa acgcgtagta ataaattttg tggtttcagt tccggttttc tgcatttttc
     4621 tggccaatga ttgcacgtaa atttcggcga tacgtttgga agctcccatc acattggtcg
     4681 gattcactgc tttatcggta gaaaccatga caaaccgtga aactctatac ttcaatgcca
     4741 aatcggccat aattttggtg cccagcacat tcacctgaac actttcacag ggaaaatctt
     4801 ccatcattgg aacatgtttg taggctgcag catgaaatac aaaatcggga cgatataaat
     4861 ccataatata ctccatccgc tctttattcc gaacatcacc caatacaaaa ttaaaatgtt
     4921 gagatgtaga aaaggcttgc aattcgattt tcaggttatg aatggccgtt tctccattat
     4981 ctaccaacac aattaacttt ggagtaaaat tacacaactg acggacaatt tcactgccaa
     5041 tggaacctgc tgctccggta accatcacag ttttcgattt caattcggca gcaatattgt
     5101 caaaagaaat ctgaatcggt tctctctcca ataaatcttc tattttcaac ggtttgattt
     5161 gtctcacgga aggcatttct ccttcccaaa tatccatcgg ctgagatgtt agcaatgaaa
     5221 tatgatgctg aataaatata tccaaatcca catccggatt aatttccttc atttttatgg
     5281 gagaaacaat aacggtattt actttattgg caactttagt tttaatagta tgatcatcat
     5341 gtttatacac tctcaatccc attaccattt tgttgacaat attttcattg ccatcaatga
     5401 aaccaaccaa tttatattgt tttttagaat tcacactgga aagaatcatt ctggctatac
     5461 ccagacccgc ctcatcgatg ccataaatca tcaccggaat gatctgagcc gaggtttcaa
     5521 caaaataatt gaaaatattt cttacggtga gcctgagacc gaataacaga ataaaactaa
     5581 taacaccata aatcagaatg gatgcataat aaaaaagtgg tgcaggaaaa taaagagaac
     5641 aaacaaagtt caacgctgag aggaaaatac aattaccaaa tattgccaaa aaaagtttgg
     5701 cagcatctgt cattgtagaa tatctgagga tgccagaata agtatgaaaa agccagaaaa
     5761 aaacactttg cgtaaaaagc aataaaatca aacgtggaaa aagaggtaaa agaagttggt
     5821 caggcaagtg ttttatgaaa gatgaagcaa cgagataagc tgctaaaaaa gatatggcag
     5881 atattgtaat atctataaat aaaacggccc agcgaggtaa atattttaaa gaagccaggt
     5941 gagagaacgt atccgtattg tttaaaaatt tgaatatctc tttcataaaa aaccaaaagg
     6001 ataaattttt gtaaggttat ttcctgtgca cagtcaaaag caatgcaaag ataagtttaa
     6061 aaatttaaaa atataaagtt cttgtcaata aatttcgaaa aaaaaatcga agggagtgaa
     6121 ttaaagctga attataaaca gtatacagtt aaaattgttt caattttaaa aaactaattc
     6181 catagattgg caaaaaggct tgatataaaa tttttttacg ataagttcag acaaaataca
     6241 gaaagtgggt taagaaaatt tatttttcct taaaaggaag cagttctttt cggaaaatat
     6301 agaaagagtt aagtttataa ttgcacatcg tcataaaaag atgtaatttt acgacaattt
     6361 ttaaaacaaa cccattaata ttttttaatt tctaattgat tcaaaaaact atatgaccaa
     6421 attaaccaaa gaagatgcgc tcctttatca ttcgcaagga aaaccgggaa aaatcgaagt
     6481 tgttcctacc aagcctcaca gttctcaaag agatttatcg cttgcctatt cgccgggcgt
     6541 agcggagcct tgtttggaaa tagaaaaaga tcccgctact gtttacgatt acacaaacaa
     6601 aggtaatttg gtagcagtaa tttccaacgg tacagccgta ttggggctgg gcgatattgg
     6661 cgcgatggca gggaaaccgg ttatggaagg aaaagctttg ctgtttaaaa ttttcgccgg
     6721 tattgatgtt ttcgacattg aagtaaacga aaaagatccc gaaaaattta ttcaggcagt
     6781 aaaagctatc tcacctactt ttggcggtat caacctggaa gatattaaag ctccggaatg
     6841 tttcatgata gaagaacgat taaaagcaga actggatatt cccattatgc acgacgatca
     6901 gcacggaaca gccattattt cgggcgccgg actgattaat gcacttgaaa ttgccggcaa
     6961 aagaatggaa gacgttaaaa tcgtcgttaa tggagccgga gccgcagctg tttcctgcac
     7021 aaaattatat atcagattgg gcgctaaacc tgaaaatatt gtcatgctcg actcaaaagg
     7081 agtaattaac aaacaacgca ccgacctgaa tgaaacgaaa aaagcctttg ctaccgacag
     7141 agaaattaat acactggaag aagctgtaaa gggagcagat gtatttttag gtttatcggt
     7201 tgccggcgtt ctgtctattg aaatggtaag atcaatgaat gaaaatccca ttgtttttgc
     7261 cttagccaat ccaaatcctg aaatatctta tgaagatgct atctcggcac gcgaagatat
     7321 tatttttgca accggtcgtt ccgatcatcc caatcaaata aacaacgtac tcggatttcc
     7381 ttatattttc cgtggggcac ttgatgtgag ggcaacctgc atcaatgagg agatgaaatt
     7441 ggctgccgtt tatgcacttg ctgaattggc aaaaaaacca gttcctgacg tggtgaatgc
     7501 tgcctacaac ttgaaaagat taagttttgg acgcgattat attattccca aacctctcga
     7561 tccccgctta cttaccattg tggctccggc tgtagctcga gcagcgatgg aatcgggggt
     7621 tgcacgcaaa cacattaccg actgggccgg ctataatgca aaactccgtg aaacgatggg
     7681 ttatgacgat aaaatgctgc gaagctttta cgaaacagca cgtcaaaatc caaaacgggt
     7741 cgtattttcc gaagcaaacc atctgaatat gcttaaagct gccgaaatgg cttacaacga
     7801 aggtgtttgt ttccctgttc tgttgggtaa tgaagaaaaa atcaacagca tagcccgcga
     7861 atacaacatc gacttgtcag gaatcgagat aattaatctc cgccacgaca gagaagaagc
     7921 aagacgtatt gcctatgccg aaaaacttgc aaaaaaactg ggacgcgaag gaatgactta
     7981 cgatgaagct tatgaaaaaa tgtatgaacg caattatttc ggcatgatga tggttgaaac
     8041 cggagaagcg gatgccctta tcactggagt ttatacaaaa tatgccgatg ccattcaggc
     8101 agcaaaggac gttatcggca tcaggcctgg gtccaaacat attgcagcca tgcacattat
     8161 gaatacaaaa aaaggaactt ttttcatggc cgacacattg tttaaccgtc atccttctac
     8221 cgaaacttta atcgatattg ccaaattaac ttaccgtacg gtaaaattct ttgcacacga
     8281 accggtgatg gccatgcttt cctactccaa ttttggttcc gacaaacagg gaagtccagc
     8341 cagcgttcac gaagtagttg aaacattgca ccgcgaagta cctgaaatga tagttgacgg
     8401 tgaaatgcaa gtgaattttg cactgaacaa agaattgcgc gaccgcaagt atccttttac
     8461 cagattagca gggaaagatg tcaatacatt gattttcccc aatttaagtt ctgcaaattc
     8521 tgcttacaag ctattgctcg aaatgggaat ctcggaagct gttgggccaa ttcaaatggg
     8581 actgaataag cccgtacata ttttggatgt ggaaagctcc gtccgcgata ttgtcaatat
     8641 gactgc
//
LOCUS       DAOTAX010000067         8363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_7265_length_8363_cov_29.066562, whole genome shotgun sequence.
ACCESSION   DAOTAX010000067 DAOTAX010000000
VERSION     DAOTAX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7265_length_8363_cov_29.066562"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..425)
                     /locus_tag="PLM04_07110"
     CDS             complement(129..425)
                     /locus_tag="PLM04_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02878.1"
                     /translation="MKAIFIVFDQGFYQQILESLYINHHVKGFTAWETVKGRGSKTGE
                     PHYGSHAWPSLNSAIITVVAEEKVKPILDDLKALDATSDVMGIRAFVWNIEDGM"
     gene            complement(605..3736)
                     /locus_tag="PLM04_07115"
     CDS             complement(605..3736)
                     /locus_tag="PLM04_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HON02879.1"
                     /translation="MSLYENSVKRPIMTSLIFVGVIILGLYSLSRLPIDLLPDIEPNI
                     LMVATSYTGASALDIENNVTRPLENTLNTVENLKNIRSNSKENTSVIILEFEFGYDLD
                     VLANDVRDKLELVKPYLPEGASNPVIFKFSTDMIPIVMISATADKSLPALYKILDENV
                     ANPLARINGVGSVSINGAPEREIQVYVDPVKLEAYGLTVESIGQNIRLQNINTPSGSM
                     DIGNKTYTVRVQGEFKDPSELSKVVVGSFNGKNVYLSDVAIINDGHEERVQESYTNGV
                     QGATIIIQKQSGANTVEIANAIRAKLPQIEKNLPSDIKLNIINDTSENIKNTIAGLEE
                     TILYAFLFVILVVLFFLGRWRATIIVILTIPISLIAAFIYLSGTGGTLNIISLSSLSI
                     AIGMVVDDAIVVLENITKHIEKGSRPNSAAIHGTNEVATAVVASTLTIVAVFFPLTLV
                     GGLAGILFAQLGWMVTIIMVVSLVCALSLTPMLSSKMLRLNPHHSRMFTVVYSPIVKF
                     LDNVDAWYANIVNWSVRHRWQTVAIAAGFFVLSLIPLIGIGTEYIPAQDNSRISATIE
                     LPVGTRMEISKKTAENLTELWREKYPEIQTINYSVGQASSSNIIGSIQNTGSHIISFT
                     LRLSDTNERNRTIFEIADSMRVDLKSFLEINKANVTAGGAMGTMGGQSTLDIEIYGYD
                     FNETDKVAMELTNRINNIQGLVNTTISRGDYSPEYQVEFDRDKLALNGLNIGTASTFL
                     RNRINGLTASLYREQGDEYTIRVVYAPEYRQSIEDVENILIYNTMGQAIRLKDLGKVI
                     ETLTPPYIERKNRERVITVSSTISGTTLDKAVQAINTELKNMEIPTGVDTQISGSYED
                     QQESFADLTTLLLIIIILVYIVMASQFESLTYPFIIMLALPFAFSGVVLALFLTGGTL
                     NLMSMIGIIMLVGIVVKNGIVLVDYINLNRERGMGIVRAIVSGGRSRLRPVLMTTFTT
                     ILGMIPLAISSSQGSEIWKPMAITIIGGLTVSTILTLLIVPTLYAIFAGFGIKRTYNK
                     YKKGTNKLQQSEK"
     gene            complement(3804..>4523)
                     /locus_tag="PLM04_07120"
     CDS             complement(3804..>4523)
                     /locus_tag="PLM04_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HON02880.1"
                     /translation="NLEIQVENYQRMYNRVAELFSVGGASQQELDNAKLQLDIAKTNL
                     QNLSENTYLLSPTAGVVTARNYDNGDIFNGQIPILTVMQINPVKLLINVSESNYAQVK
                     IGMPATIKFDIFEGETFQGKISLIYPTIDERTRSFQVEIKMNNANYKIRPGMFARVEI
                     NFGSARRIIVPDMAVIKQSGSGEYFVYVYKDGSVIYQRVELGKRLGKEYEIISGLNDG
                     DLVVVSSQSKLADGKKVNLQK"
     assembly_gap    4524..4623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4755..6107)
                     /locus_tag="PLM04_07125"
     CDS             complement(4755..6107)
                     /locus_tag="PLM04_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HON02881.1"
                     /translation="MTANSLRKSIFIVLCVIALLPFSLKAQDTLIVSLDKAIEIALSE
                     SPTIKVANKEIQRVDYSSKEKISALFPVISASGQYSRAVKKQKLFFNIPGMPARPDGI
                     EVGQDNTFSGGISAAMPLIAPTLWATIKMSELDAQLVMESARSSKLSLVNEVSKAYYA
                     ILLAQDSYDVFKKSYDIAVENAKIIENKYKQGTVSEFEWIRADVQVKNANANLVSAEN
                     AVNLSVLRLKMLMGIDMFIGLKVEGNLSNYEANKYADVLSIDTTTLKSNSDLKQFDIK
                     AQQLKQSLKIQKSTNLPTLSAAINYQYMSMADDSIAFSNYHWFPTSTAALSLSIPLFQ
                     GGGKLFKQKQIEIQVEEMKYQRDNLRRGLELQAMSYLDNINQSLKVIESNKEGLRQAE
                     KALSISQKRYEVGAATYLDLSNAELAYTQAGLSYNQSIYNYLSAKADLEKLLGNQIIF
                     "
     gene            complement(6146..6757)
                     /locus_tag="PLM04_07130"
     CDS             complement(6146..6757)
                     /locus_tag="PLM04_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HON02882.1"
                     /translation="MKNTEEVILQKSLDLFIEFGIRNVSIDTICNELRISKKTFYSYF
                     KQKEDLVDAILTYEEKINIEEMKKRFDDKNAIESLISVLKEIKKNIDCQPKLMWMDIK
                     KYYPKVYEKHEMRRRSIIRKDFEKNLQQGVKEGFYRDDLDIELLSMLHSYQLKNIMTT
                     MSQYPKKYSKKRLVDFFIDIMIHIIANEKGLEYVRKNYYESEP"
     gene            complement(7625..>8363)
                     /locus_tag="PLM04_07135"
     CDS             complement(7625..>8363)
                     /locus_tag="PLM04_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HON02883.1"
                     /translation="PQQLIDSIQYHKKYRGDFLPPANYQLVEQVEGRGVYSFCMCPGG
                     HIVPAGSGTKEIVVNGMSASKRNSPFANSGIVVEIRPEDIPSDFSGCGPLASLKFQKH
                     LEKLAYINNGGLNLTAPAQRLTDFVNGKLSDNLPTCSYLPGLISSPLHFWMPDFLTKR
                     LQEGFKKFDKKMHGFLTREAVIVGVESRSSSPIRIPRDKETYQHPQIKGLFPCGEGSG
                     YAGGITSSALDGENAAIAVYKSRLYHN"
BASE COUNT         2557 a         1587 c         1365 g         2754 t
ORIGIN      
        1 cgtatctgta atttcatctc gataaaatca attttctatt attaaaaagg cactcattat
       61 tagagcgcct tttcacttat tgagcaatgc agtaaaaata aaaaataaaa ttcaagcaaa
      121 aacaagaatt acataccatc ttcaatattc caaacaaatg cacgaattcc catcacatcc
      181 gaagttgcat caagagcttt tagatcatcc agtataggtt ttactttttc ttcagccact
      241 acagttataa ttgccgaatt cagggatggc catgcatggc ttccataatg aggttcgccg
      301 gttttagacc cgcgtccttt tactgtttcc caagcggtaa atcctttaac atgatgattt
      361 atataaagcg attcgagtat ctgctgataa aaaccttgat cgaaaactat aaatattgct
      421 ttcataaata ttgttttttc tgattaacaa tcgggattgg accagatata ttaaataaga
      481 acttaaccaa acccagtcca atctcaatct tcttttttga attgtataaa aacattttgt
      541 ttaacttaaa atctttacct acttttttat attttcttgg tcaggtaaaa attcaattaa
      601 attattattt ttcggattgt tgcaacttat tggttccctt tttgtacttg ttgtaagtgc
      661 gtttaattcc gaatccggca aaaattgcat acaacgttgg cacaataagt aaagtcagaa
      721 tagtggaaac tgttaagcca ccaataatgg ttatggccat tggtttccat atctctgatc
      781 cttgagatga acttattgcc aaaggaatca taccaagtat agtagtaaaa gttgtcatca
      841 atacgggacg caaacgggaa cgacctccgc tgacaattgc cctgacaatt cccatgccgc
      901 gttcacgatt taaattgata taatctacca gaacaattcc gttttttact acaataccga
      961 ccaacatgat aattcctatc atggacatta aatttaacgt accacccgtt aaaaacaatg
     1021 ccagcacaac tcccgaaaat gcaaatggta aagcaagcat aatgataaac ggataagtca
     1081 aggactcaaa ttgtgatgcc ataactatgt aaaccaaaat gatgataatc agcaacaaag
     1141 ttgttaaatc agcaaatgac tcttgttgat cttcataaga accggaaatc tgtgtgtcaa
     1201 ctcctgttgg tatttccata ttcttaagtt ctgtgttgat ggcttgcact gctttatcga
     1261 gagtggttcc cgaaatagtt gaggaaaccg taatcacacg ttcacgattt tttctttcta
     1321 tatagggagg tgttaaggtt tctatcactt tccccaaatc tttaagccgt atagcttgtc
     1381 ccatagtatt gtatatcaat atgttttcaa catcttctat actctgtctg tattcgggag
     1441 catatactac acgtatggta tattcatcgc cttgttcacg atacaggctg gctgttaaac
     1501 cattaattct gttccgcaaa aaggtggatg cggttccaat gttcaatccg ttaagtgcca
     1561 gtttatctcg atcaaattct acttgatatt cgggtgagta atcacctcga ctaattgttg
     1621 tattaaccag tccctgaata ttgtttattc tgttagttaa ttccattgcc actttatcgg
     1681 tttcgttgaa atcgtagcca tatatttcta tatccagcgt cgactgtcct cccattgttc
     1741 ccattgctcc acctgcagtt acatttgctt tgtttatttc caaaaaggat ttgagatcca
     1801 cacgcatact gtctgcaatt tcaaaaatag ttctatttcg ttcgttcgta tccgacaaac
     1861 gcagggtaaa agaaatgata tggctaccgg tgttctgaat tgaaccaatg atgttggaac
     1921 tgctggcctg ccctacggaa taattaatgg tttgaatttc aggatatttt tctctccaaa
     1981 gttctgtaag attttctgct gttttcttag agatttccat ccttgtacca acaggtagtt
     2041 ctatggtggc actgatgcgc gaattgtcct gagcaggaat atattctgtt cctataccga
     2101 ttaagggaat caaactcaac acaaaaaatc ctgctgcgat agcaactgtt tgccatctgt
     2161 gacgaaccga ccagttgact atattggcat accacgcatc cacattgtcg agaaatttta
     2221 caatcggtga ataaacgaca gtaaacatac gagaatggtg aggattaagt cgcagcattt
     2281 tcgagctcaa cattggagta agcgacaagg cacaaaccag cgaaactacc ataataatag
     2341 taaccatcca gcctaattgg gcaaaaagaa tccctgccaa tccccctacc agcgtcaagg
     2401 gaaagaaaac ggcgactatc gtgagagttg aagcaacaac agcagttgca acttcgttgg
     2461 taccgtgtat tgctgcgcta ttcggtctac ttcctttttc aatatgcttt gtaatatttt
     2521 caagtaccac aatagcatcg tcaaccacca tacctattgc aatcgataat gaactgagcg
     2581 aaatgatatt tagggttcct cctgtgccgc tcaaataaat aaaggcggcg ataagtgata
     2641 taggaattgt aagaatgacg ataatggtcg ctctccatct tcccaagaaa aacaacacca
     2701 ccaaaataac aaagaggaaa gcatataaaa tagtttcttc cagaccggct atcgtgtttt
     2761 taatattctc tgaagtgtca ttaataatat tcagttttat atcagaagga agattttttt
     2821 ctatttgggg taatttggct ctgatggcat tggcaatttc caccgtattt gcaccgcttt
     2881 gtttttgaat tatgatagtt gctccctgaa cgccattggt gtaactttcc tgaacacgtt
     2941 cttcgtgtcc gtcgtttatt attgccacat ccgacaaata tacattcttc ccattgaaac
     3001 ttcccactac aactttactc agctcgctag ggtctttaaa ttctccctgt accctgacag
     3061 tataggtctt atttcctata tccatcgaac cacttggagt gttgatattt tgaagacgga
     3121 tattctgacc aattgattca acagttaaac cataagcttc aagtttgacc ggatcaacat
     3181 atacctgaat ttccctttcg ggagctccgt tgatggaaac agaaccaaca ccgttgatac
     3241 gtgccagagg attggctaca ttttcatcaa gtattttata aagtgccggt aaacttttat
     3301 ctgctgttgc agaaatcatc acaatgggaa tcatatcggt actgaattta aaaattaccg
     3361 gattactggc tccttcaggc aagtagggtt tgaccaattc gagcttgtct ctgacatcat
     3421 tggctaatac atccaagtcg tatccaaatt caaattcgag aataataaca gaagtgtttt
     3481 cttttgaatt ggaacgaata tttttcaaat tttcaactgt attcaatgtg ttttcaagcg
     3541 gacgggtaac attgttttca atgtccaatg cacttgctcc tgtataactg gttgctacca
     3601 ttaaaatatt gggctctata tcgggaagca aatcaatagg tagcctgctt aatgaataca
     3661 accccaaaat aattactcca acaaaaatca gagatgtcat tatgggcctt ttaaccgaat
     3721 tttcgtataa actcatagat tttaaatttt gaactaagtt attttaatac cgttttttca
     3781 aaaacttttt tcggtaaaaa tttttatttt tgtaaattca ctttcttacc atctgccaat
     3841 tttgactgac ttgaaaccac aaccaagtct ccatcattca ggccggaaat aatttcatat
     3901 tctttgccca agcgcttacc taattccact ctctgataaa ttaccgatcc gtccttatag
     3961 acataaacaa aatattctcc agaaccggat tgcttaataa cagccatatc cggaacaata
     4021 attctccggg cagaaccgaa attgatttca actcttgcaa acatgccggg acgaatttta
     4081 taattcgcat tattcatttt aatttctact tgaaaggatc gagtgcgttc atcaatagtt
     4141 ggataaatca aactgatttt tccctgaaag gtttctcctt caaaaatgtc gaatttaatg
     4201 gtagccggca ttccaatttt tacctgtgca taatttgatt ccgatacatt gatcagtagt
     4261 tttaccggat taatttgcat aacggtaaga atggggattt gtccgttaaa aatatcacca
     4321 ttgtcgtagt tacgtgctgt aacaacacct gctgtcgggc tgagcaaata tgtattttcg
     4381 gataaatttt gcaaattggt tttagctata tccaattgca atttggcatt gtcgagttcc
     4441 tgctgtgaag cacctccgac gctgaaaagt tctgccaccc gattatacat tcgctgataa
     4501 ttttcaacct gaatttcgag gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnncctgatt ttccgcatga caccagccca attgttaagg ccataattaa tatccctaat
     4681 ttatttatcc ttttcatttt aatgtcattt atttggttgt tattataaat tgaaatctgt
     4741 attatatttt aattttagaa aataatctga ttgccaagta gtttttccag atcggctttt
     4801 gcggacaagt aattgtaaat agactgatta taagataagc ctgcttgggt ataggccaat
     4861 tcggcattgg acaaatcgag ataagttgca gctccaactt cataacgttt ttgtgaaata
     4921 ctcagtgctt tctccgcctg tcgcaatcct tccttgttcg attctatcac ttttaaggac
     4981 tgattaatgt tatccaaata cgacatggct tgtaattcca aacctctcct gagattgtcg
     5041 cgctgatatt tcatttcttc tacctgaatc tcaatttgtt tttgcttaaa gagttttcct
     5101 cctccttgaa acagaggaat agaaagagaa agtgctgccg ttgaagtggg gaaccaatga
     5161 taattggaaa atgctatcga atcgtcagcc atactcatat actgataatt gatagctgcc
     5221 gataaggtag gaagattagt ggatttctga atcttcaaac tttgtttgag ttgctgggct
     5281 ttgatatcga attgtttcaa atcgctatta cttttcaggg tagtggtgtc aatggatagc
     5341 acatctgcat atttgttggc ttcatagttt gatagatttc cttcaacttt taaaccgata
     5401 aacatgtcaa ttcccatcaa catttttagt cttaaaacac tcaaattaac tgcattttct
     5461 gctgaaacca aattggcatt ggcatttttg acctgcacgt cggcacgaat ccattcaaat
     5521 tcggatacgg ttccctgttt gtatttgttt tctataattt ttgcattttc taccgctatg
     5581 tcgtaacttt ttttaaatac atcgtacgag tcctgcgcca acaaaatagc ataataagct
     5641 tttgaaactt cgttaacaag tgaaagtttt gaagaacgtg cagattccat taccaactgg
     5701 gcatcaagct ccgacatttt tatggttgcc cagagagtag gcgcaattaa aggcatggca
     5761 gccgaaattc ctccactgaa agtattgtcc tgtcctactt caattccgtc aggacgtgcc
     5821 ggcataccgg gaatattaaa aaacaatttc tgttttttta cagctctgga gtattgcccc
     5881 gaagccgata tgacaggaaa taaagcggaa attttttcct tgctcgagta atccactctt
     5941 tgaatttcct tgttggcaac cttaatggtc ggactttcac tcaacgctat ttctatcgcc
     6001 ttatcgagtg aaacaatcaa tgtatcttgc gctttgagtg agaaaggcaa aagcgctatt
     6061 acacaaagaa caataaaaat tgattttctt aatgaatttg ctgtcatatt ctttatttat
     6121 tttttggtta taccaatttt aaaacttaag gttcactttc ataatagttt tttcttacat
     6181 attcaagtcc cttttcattg gcaataatgt gtatcataat gtctatgaaa aagtcaacga
     6241 gccgtttttt tgaatatttt ttggggtatt ggctcatggt ggtcattatg tttttcagtt
     6301 ggtatgaatg gagcatcgaa agcaattcaa tgtctaaatc atctcgataa aatccttctt
     6361 ttactccttg ttgaaggttc ttctcaaaat cttttctgat aatagaacgc ctgcgcattt
     6421 cgtgcttttc gtaaacttta ggatagtatt tttttatgtc catccacata agtttaggtt
     6481 ggcagtcgat gtttttctta atttctttca aaacggagat aagtgattca attgcatttt
     6541 tatcatcgaa tctttttttc atttcttcaa tattgatttt ttcttcataa gtcaatattg
     6601 cgtccaccaa atcttccttt tgtttaaaat aactgtagaa agtctttttc gaaattctca
     6661 gctcattgca aatggtatca atggaaacat ttctgatacc aaattcaata aacaaatcca
     6721 acgatttttg aagtataact tcttcagtat ttttcatttt gcgaaataaa aaattttcat
     6781 tgcaaaatta attaaaaaat catcgtacat aagcaaaaaa acaataacaa aaagttaaat
     6841 attattttct tttatataag aatctgttta tcaataattt aattatatgt aattaaaaac
     6901 ttttagaatt taatagtttc caagtaaata tctatataaa aatttgactg tacaatattg
     6961 ttaaaataaa ttatcaaaag catttcattt taaatttata cttttacgtt acagcaagga
     7021 tggcaattca aaaaatcatg gtaacattta aggattgaat cattcaatta acaattaagc
     7081 cataaaatgg ttcagaattt tgaatgaaaa ttttcatcaa aaattaagcc aaaaccaata
     7141 ggtacataaa aaaatgggag acaaaataat ttttaccatc attttattta tttcaacccg
     7201 aaatcctgtg gctgaagtcg gtgtttttta tcagataata aaaacacaat tgttctccaa
     7261 ttaacttcgc tccattttta gtccaaccgg caacaattcg tagtttatgt tcaatgaaat
     7321 ttcgattatg aaacccatat gtattacata cacaaccacg aatgaatata gtattcagac
     7381 atgtggcttc catcggacca ttaaaaaata aattatattc tgttgaaata aataaataaa
     7441 ttcctttatt gcttattaaa cgatttttcc tatcgtaatt taaggcgtct taaaccgaaa
     7501 gatacttttt tctaaacata agcacaccta tatgttctat tttattgata tttaattaaa
     7561 tagatagaat agaaaaaatt tagaggagag ctattcaata atttcagtcg caaacaaaat
     7621 ggaactaatt atgatataat ctacttttat aaacagctat ggcagcattt tctccatcta
     7681 aagctgagga ggtaattcct ccggcatagc ctgaaccttc accacagggg aaaagccctt
     7741 ttatttgagg atgctgataa gtttccttat cacgtggtat ccttattgga gaggaagaac
     7801 gcgattctac accaacaata actgcttcac gggtgagaaa tccgtgcatt tttttgtcaa
     7861 attttttaaa tccttcttgt aaacgtttag tcaaaaaatc aggcatccaa aaatgtaacg
     7921 gggaagagat caaaccagga agataggaac aagtaggcaa attgtcggag agttttccat
     7981 tcacaaaatc cgttaatcgc tgagccggag cagtcagatt caaaccacca ttattgatat
     8041 aggccaattt ttctaaatgt ttttgaaact taagtgaggc aagtggccca caacctgaaa
     8101 agtctgaagg aatatcctca ggtcttattt cgacaactat acccgaattg gcaaaggggg
     8161 aatttctttt ggatgcagac attccgttga caacaatttc ttttgttcca cttccggcgg
     8221 gcacaatatg tcctcccgga cacatacaaa aggaatatac accacgtccc tccacttgtt
     8281 caaccaactg ataattggcc ggagggagaa aatctcctcg atacttttta tgatactgaa
     8341 tactatcaat caattgttgg ggg
//
LOCUS       DAOTAX010000068         8115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_7540_length_8115_cov_27.704963, whole genome shotgun sequence.
ACCESSION   DAOTAX010000068 DAOTAX010000000
VERSION     DAOTAX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7540_length_8115_cov_27.704963"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLM04_07140"
     CDS             <1..657
                     /locus_tag="PLM04_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HON02884.1"
                     /translation="RVSGILASLSKNTSTYGHFQEIVDHNIPLVFFDRICIGISTDRV
                     VVDDYAGAFAAVEYLIQTGCKKIAFYTSPLHLEISKNRRNGYLDALHKYGLPVDESLI
                     RVCDTREEAIIITPEILDRPDRPDGFFAINDHCASGILYAAKLAKLHVPQDISICGFS
                     DGELAKACDPMLTTVEQHGYEMGISAAELLISKIKGETQGKYTNKIIKTNLIIRGTTR
                     "
     gene            733..2100
                     /locus_tag="PLM04_07145"
     CDS             733..2100
                     /locus_tag="PLM04_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON02885.1"
                     /translation="MQEFTFARKKVKPHLSFFRILSLSMGFLGIQFGYALQNANASRI
                     LQGFGADIEQLSWFWLAAPLTGMIVQPIIGHYSDHTWTRLGRRRPFFLAGAFLAAIAL
                     VLMPNAGVFADVLPAMFVGAGFLMIMDASINVAMEPFRALVADMLPAEQTTVGFSVQS
                     FLIGIGAVIGSWLPYVLAEWLGIAKTAPEGQVPPNVIFSFYIGAVVLIVTILWTVFTT
                     KEYSPEELQAFESEEERTEKKNPKFSDIFKDMATMPKTMKQLSITQFFTWIALFGMWV
                     FTTPAIAQHVYGLPVTDTSSPTYQDAGNWVGILFGVYNGVAMFYALALPAIARNIGRK
                     LTHSFSLICGAAGLLSIFFINDPQWLILSMIGVGIAWGSILSMPYAILAPSLPPAKIG
                     VYMGIFNIFITVPQIVNGLFGGMVVKRIFDSQAIYALIMSGCFLLIAAISVLWVEDKA
                     EIRHR"
     gene            complement(2228..6139)
                     /locus_tag="PLM04_07150"
     CDS             complement(2228..6139)
                     /locus_tag="PLM04_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HON02886.1"
                     /translation="MRKIIKHIIRTIGFLIITPVILLLLALGALQTPFVKSLLVDFAE
                     LQAEKVLNGQLHIGKLEGNFINGLRLKQILLTNHSDTLIWIPMLKIDYQLAPLVSGNI
                     SIHSLTIDRLQVKLMQQKNGRWNFQELIKTPSQPTEKTSPFKMRITLENLQLNNAQIF
                     ILSENPSLPEKISDLHLKCNGEYSDKQSKLFMEEFRLTSHQPDLQIKELKFNFVQESE
                     ILHLSDFFLRTGNNTLTANSKIDISASETSFLSVKTAPLFLNEFADFFSAPLQENIHP
                     LVHVESIMQDDNLRLTLNLSEKKEKIEINLQVSKITEIIRQPKLTDNVTYTLMANVDQ
                     MELNKWINLPEPNILLNGEIFINGKGISLNTMEMGIKGDFGKLKINNQDLHKLNFNFD
                     YASKKLTGQLFAESPFGNLYLQSAIKEPTESIPSYKFLLVTRKLNPSTLFPAMKGSSD
                     LNMKIELEGSGFDPLQMTAYAKIQVDTSSISGWTIHSLQSNLNFQQQNFFIENFSVET
                     ASAHLSANGIYSIKGNSDAFLNLTLNNASEIIKFTGVENLNGRGKIAGHFHGTPKKFL
                     TDLNLTLFDTQFQTYSAEEVTAHITAQIPNDRNFSVSGNLNAKNLNLGGIELQSIQLV
                     SNADSKNIQLSSIAESGELNTSFKSAIHLDNPLLVELSDFRINYRNNSWKQGTDTALF
                     YLSETDYRLEHLKLISEDADTLQTLSAEGKISKNGEQNFQISLSNLDFAKISETFFPE
                     QSLQGKINFNAQLMGEASSPVAEAKFRLNNFALQNYYAGNMTGTLQLTQQQLNTKVNI
                     NLPDSGKLSADAKIPVNFRLDSMQFNIPSYENENFDFNLFANKIPLTLLQSFLPDGQY
                     EGIIQTSMHAGGNIKEPELKGKIEITDGKIKMPQYGINYPHFMAGISMDNNKIKIDTF
                     LITSRDGRMNINGNMSPPTFLSKSTNAPSPLANVQIHFANFRPIDHKNYNMAVSGDAL
                     LKGNADSLLFSGDINIPQAEINLPSLLQLVGKTSAPQLPEPLLIKELKKMQPEDSLVI
                     TLRPRNEESKKEETDFLKKLQGNLRVKIPRNTWIKNQDMRVELSGDVQLMKHKEFFEL
                     FGSVEVLRGQYNLLGKVFIIESGTISFQGGEKINPLLNFTAAYNFRDSNRQQKKLLAN
                     ISGEMMSPEIKFTMDNQQIDEGDALSYILFGAKLDALTSGQRENINQTFDALGLTESL
                     AASGLSSQLTKLLSNTLNVDYIEVKSKGSFESGSLEVGKYLTNKIFINYVRNFGNYQR
                     DENFSEYEIRVEYEILKFLFLQLTTSPVKNGIDIVIKPTKK"
     gene            complement(6147..8012)
                     /locus_tag="PLM04_07155"
     CDS             complement(6147..8012)
                     /locus_tag="PLM04_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HON02887.1"
                     /translation="MPYRFKFLEFNIFRQGKAVVLFLFYFCAIAAKAQNGYQIEKITI
                     VGNQTFSKSELLEKTNLATYNFLQRTFLKKEPVIFNENLMELDIERMTRFYQTEGFPF
                     VEVQVDSLATNHKKQKVEIYIKIEENTPIFFGEIGFDIESQSISEEKKDTLLHLIKKH
                     IVSKNGRRFRDKDVHEDKSRIEQIFINSGYVYVQVDHLINLSLDSYRADVVYVIRPFE
                     ICFFGKTEIVGNQNIKESLIRRQLAYTEGEIYSNKKLDKTRKQLFDLQLFRVASLQPQ
                     IDASSTRNSIPVKLTIEETPQWNAQIGLGYGTEDQFRTFLDLSFHSLWGDASRFHLYA
                     KHSALEPYHFSLSWIQPQLFSKNISMRLNPFLRRQAEPGFDIRTYGLNLPFGYSFSDK
                     INLTLSYYLENIKQYSEVDNLELINPESKDFLYNKSGLLTTFQYNNAFPQINPERGEI
                     ISIGIKSNGYLFGSDFDYTKIWIDTRKYLQINNFSCAARLMGGTVFSSNVIEYVPVED
                     RFYSGGSNSVRGWSRWALGPKRDDGSPLGGKSNLELSLEIRHPLFWLLESAVFLDAGN
                     VWASSFHFPLDKLAYSAGTGLRIKTPVGAIRLDISVPLWNEKKSVQVFLNVGQAF"
BASE COUNT         2311 a         1419 c         1617 g         2768 t
ORIGIN      
        1 cgtgtttcgg gtattttggc cagcttatct aaaaatacgt ccacttacgg ccattttcag
       61 gaaatagttg atcacaatat tccattggtt tttttcgata ggatttgcat tggcatcagt
      121 acagataggg tagtagtgga tgattatgcg ggggcttttg ctgccgttga atatctgatt
      181 caaacaggtt gcaagaaaat tgccttttac acttcaccgc ttcatctcga aatttctaaa
      241 aatcggagaa acggttatct ggatgcgctc cacaaatacg gcttacctgt ggatgaatcg
      301 ctgattcgag tttgcgatac gcgggaagaa gccatcatta ttactccgga aattttagat
      361 cgccctgatc gtcctgatgg tttttttgcc attaacgacc attgtgcatc aggtattttg
      421 tatgcagcaa aattggccaa gttgcatgtt cctcaggata tttccatttg tggtttttcg
      481 gatggagaat tggcaaaagc ctgcgaccct atgttgacta ctgtagaaca gcatggttac
      541 gaaatgggca tcagtgcggc cgaactgttg atttccaaaa tcaagggtga gacacaagga
      601 aaatatacga ataaaataat taaaaccaat ttaataatca gaggtactac gcgctgattc
      661 aatggttttg agacattaat ttcccggaat ggtttttatt aaaaattttc aatactcata
      721 catttgaaaa gtatgcaaga atttacattt gcgagaaaaa aggtgaaacc tcatttaagt
      781 ttttttagga ttctttccct cagtatggga tttttgggta ttcagtttgg ctatgcgttg
      841 cagaatgcca atgccagccg aatattgcaa ggatttggtg ctgatattga acaactttcc
      901 tggttttggt tggctgctcc acttacagga atgattgttc aacccattat tggtcattat
      961 tcggatcaca catggacacg actgggcagg cggcgaccgt tttttttggc aggtgctttt
     1021 ttggctgcta tcgctttggt attaatgccc aatgccggtg tttttgccga tgtgttaccg
     1081 gccatgtttg ttggtgcagg ttttctaatg ataatggatg cttccattaa tgtggctatg
     1141 gaaccttttc gtgcgctggt tgccgatatg ctacctgccg aacaaactac agtcggattt
     1201 tcggttcaga gttttctgat agggattggt gctgtcattg gttcatggtt gccttatgtg
     1261 ctggcagagt ggttgggcat agccaaaact gccccggaag ggcaagttcc gccaaatgtg
     1321 attttttctt tttatattgg tgctgtcgtg ctaattgtta caattttgtg gacagtgttt
     1381 acaacgaaag aatactcgcc ggaggaattg caggcatttg aatcggaaga ggaaagaacg
     1441 gagaaaaaaa atccaaaatt ttccgatatt ttcaaagata tggcaactat gccaaaaacc
     1501 atgaagcagc tttccattac tcaatttttc acatggattg ctctttttgg catgtgggtt
     1561 tttacaacgc cggccattgc tcagcatgtt tatggtttac ctgttaccga tacttcttcc
     1621 ccaacttatc aggatgcggg aaattgggta ggaatacttt ttggcgttta caatggtgtt
     1681 gccatgtttt atgcgctggc tttacccgca atagccagaa atataggcag aaaattgaca
     1741 cacagctttt ccttgatttg tggcgcagcc gggttgcttt caattttctt tatcaatgat
     1801 ccccaatggt tgatactttc catgattggt gtcggaatag cttggggcag cattttgtcg
     1861 atgccgtatg ccattcttgc tccatctctt cctccggcaa agatcggagt ttatatgggt
     1921 atcttcaata ttttcattac tgttccacag attgtgaatg gactttttgg tggaatggtg
     1981 gtgaaacgga tttttgattc acaagccatt tatgcactta tcatgtcggg ttgttttttg
     2041 ctgatagccg ccataagtgt gttatgggtt gaggataagg cggaaattcg acatagatag
     2101 aatattgctt agatgaattt attgtatgaa ttgtttcgat ttttttatat tttcccgacc
     2161 tttttccagc attacatatt gaaaggaatg agaagcagct ccatttcttt aaaagtgggt
     2221 acaagcctta tttttttgtg ggtttaataa ctatgtctat tccatttttg accggtgaag
     2281 tagttaattg aagaaacaaa aatttgagaa tttcgtactc gactctaatt tcatattcac
     2341 tgaagttttc gtcgcgttga tagtttccaa aattgcgaac gtaattaata aaaattttgt
     2401 tggtcaagta tttaccaact tcaagggaac cactttcaaa tgaacccttg ctttttactt
     2461 ctatataatc tacattcagt gtattactca gaagtttggt aagttgcgaa gaaagtcccg
     2521 atgcagcaag actttcggtc aatcccaatg catcaaaagt ttgatttata ttttctcgtt
     2581 gtccactggt caaggcatcc aattttgctc caaacagaat ataggaaagt gcatctcctt
     2641 catcaatttg ctggttatcc attgtgaatt ttatttcagg cgacatcatt tctcctgaaa
     2701 tatttgccag cagttttttt tgttgtctgt ttgagtctct gaaattgtag gctgcagtga
     2761 agttgagtaa tggatttatt ttttctccac cttgaaaaga aattgttccc gactcgataa
     2821 tgaaaacttt tcccagtaaa ttgtattgac cgcgcagcac ttcaaccgat ccgaaaagtt
     2881 caaaaaactc tttgtgtttc attaattgaa catcacctga aagttcaaca cgcatatctt
     2941 gattttttat ccaagtatta cgtggaattt ttacacgtaa atttccttgt aattttttta
     3001 aaaaatccgt ttcttctttt ttgctttctt cattacgggg tcgcagtgta ataaccagag
     3061 aatcttcagg ttgcattttt ttcaactctt taattaatag aggttcgggg agttgagggg
     3121 cggaggtttt tcctaccagt tgcagcaatg aaggaagatt tatttcagct tgagggatat
     3181 taatatctcc cgaaaagagt aaactgtcag cgtttccctt cagcaaagca tcgcccgata
     3241 cagccatgtt gtaattttta tggtcaatag gtctgaagtt ggcaaaatga atttgaacat
     3301 ttgccaaagg tgaaggagca tttgtagatt ttgataaaaa agtgggaggg gacatattac
     3361 catttatatt cattcggcca tcacgactgg ttattaggaa agtgtcaatt ttaattttat
     3421 tgttatccat cgatattccg gccatgaagt gaggatagtt gattccatat tgcggcattt
     3481 tgatttttcc gtctgtaatt tcaatttttc ctttcagttc gggttcttta atgtttccac
     3541 cggcatgcat ggaagtttgt ataattcctt catattgtcc gtcaggaaga aaagattgca
     3601 gtaatgtaag aggaatttta ttggcaaaaa gattaaaatc aaaattttca ttttcgtaac
     3661 ttggaatatt gaattgcata ctgtccagac gaaaattcac cggaattttt gcatcggcag
     3721 aaagttttcc gctgtccggt aaatttatgt ttacttttgt gttcaactgt tgttgggtga
     3781 gttgaagtgt ccctgtcatg ttgcctgcat aataattttg aagtgcgaag ttgtttaacc
     3841 gaaattttgc ttcggctacc ggagaagaag cttcgcccat caattgtgca ttaaaattta
     3901 tttttccttg tagcgattgt tcaggaaaaa atgtttcgga tattttggca aaatccaaat
     3961 tagagaggga aatttgaaaa ttttgttctc catttttact gatttttcct tcggccgaaa
     4021 gagtttgtaa ggtatcagca tcttcagaaa ttagttttaa atgttcaagc cgataatcag
     4081 tttcgctcaa gtagaacagc gcagtgtcgg taccctgttt ccaactgttg tttcggtaat
     4141 ttattcggaa atcggataat tcaaccagca gagggttgtc gagatgaatt gccgatttaa
     4201 atgaagtatt taattccccg ctttctgcaa tagacgacaa ttgtatattt tttgaatcgg
     4261 cattggatac aagttgaatg ctttgtaatt cgattccacc gagattcaga ttttttgcat
     4321 tcaaattacc tgatacagaa aagttcctgt cattgggtat ttgtgcagtt atatgtgctg
     4381 taacttcctc agcactgtaa gtttgaaatt gcgtgtcaaa caaggttaaa ttcagatcgg
     4441 taagaaattt ttttggagtt ccatgaaaat gtccggcaat tttgcctctt ccattcaagt
     4501 tttctacgcc ggtgaatttt attatttctg aagcgttgtt gagcgtcaag ttcagaaaag
     4561 catcggaatt tccttttatg ctgtaaatgc cattggcaga taaatgtgcc gatgccgttt
     4621 caactgagaa attttcaata aagaaattct gttgttgaaa atttagattg gactgcaacg
     4681 agtgaattgt ccatccggaa atactggagg tgtccacttg aatttttgca taagcagtca
     4741 tttgaagtgg atcgaaaccg gaaccttcaa gttctatttt catattcaaa tcggaagaac
     4801 ccttcatggc aggaaacaag gttgaagggt tcaattttcg ggttacaagc agaaatttat
     4861 aagatggaat gctttcggta ggttctttta ttgctgactg taaatacaga ttaccaaaag
     4921 gactctcggc aaataattgt cctgttaatt tttttgaagc ataatcaaaa ttgaaattca
     4981 gtttatgtag atcctggttg ttaattttta attttccgaa atcacccttt atccccatct
     5041 ccatcgtatt caatgaaatg ccttttccgt ttatgaatat ttctccattg agcaaaatat
     5101 tgggttcagg caggttgatc cacttgttca attccatttg gtctacattg gccatcagag
     5161 tataggtaac attgtcggtc aatttgggtt gccgaattat ttccgtaatt tttgaaacct
     5221 gaagatttat ttcgattttt tccttttttt cggacaggtt taatgtgagc cgaagattat
     5281 catcctgcat tattgattcg acatgcacca gtggatgaat attttcctgc aaaggagcag
     5341 aaaaaaaatc tgcaaattca tttagaaaca agggagcagt tttaacactt aaaaaggaag
     5401 tttctgaagc tgaaatgtcg attttcgaat tggcagtcaa agtattgttg ccggtacgaa
     5461 gaaaaaaatc ggaaagatgt aatatctcac tctcttgtac gaaattaaat ttcagttctt
     5521 taatttgtaa atcaggttga tgtgaggtta gtctaaattc ttccatgaaa agtttcgatt
     5581 gcttgtcgga atattccccg ttgcatttca gatgaagatc ggaaattttt tcaggcaggg
     5641 agggattttc tgaaagaata aaaatttgag cattgttcaa ttgtaaattt tcaagtgtta
     5701 ttctcatttt aaagggagat gtcttttcgg tgggttgtga aggggtcttt attaattcct
     5761 gaaaattcca acgcccattt ttttgctgca tcagttttac ttgcaaccga tcaattgtta
     5821 aggaatgtat cgaaatatta cctgaaacta aaggtgcaag ttggtaatct atctttagca
     5881 ttggtatcca tatcaacgtg tccgaatggt tggtcaacaa aatttgtttt agacgtaaac
     5941 cattaataaa atttccctcc aacttaccta tgtgtaattg tccgttcaat accttttcgg
     6001 cctgaagttc agcaaaatca accaaaaggg attttacaaa gggtgtttgt aatgccccca
     6061 acgcaagtaa caacaggatc accggagtta tgattaaaaa ccctattgta cggatgatat
     6121 gtttgatgat ttttcgcata aggtttttaa aatgcttgtc cgacattcag aaaaacttgt
     6181 accgattttt tttcgttcca aagcggaacg cttatatcca agcgaatagc gccaacaggt
     6241 gttttaattc tcagtcctgt acctgctgag taagccagtt tatccaaagg aaaatggaat
     6301 gaggatgccc acacattacc ggcatccaaa aacacggcac tttcaagaag ccaaaataaa
     6361 ggatgtcgta tttccaaact gagctccaaa ttgctttttc ctcctaatgg agaaccgtca
     6421 tctcttttgg gaccaagagc ccagcggctc cagcccctta cagagttgct ccctccagaa
     6481 taaaatctgt cttccactgg aacatattct attacattgg aggaaaaaac agttccgccc
     6541 attaatcgtg cagcacaaga aaaattgtta atttgcagat attttcgtgt atctatccaa
     6601 atttttgtat aatcaaaatc actcccaaat aaatatccat tgctttttat gccaatactt
     6661 ataatttccc cacgttcagg gttaatttgc gggaaagcat tgttgtattg aaaagtagtc
     6721 agcaatcccg atttgttgta aagaaaatct ttactttcag gattgattaa ttccaaattg
     6781 tccacttcgg agtattgttt aatattttcc aaataataac ttaaggtaag atttatttta
     6841 tcggaaaagg aatagccgaa tggtaaattt agtccataag ttcgaatatc aaaaccgggt
     6901 tctgcctgcc tgcgtaaaaa agggttaagc cgcatcgaaa tatttttaga aaataattgc
     6961 ggttgtatcc agcttaaact gaaatgatac ggttccaatg ccgaatgttt ggcgtacaaa
     7021 tgaaatcgtg aagcatcgcc ccacaacgaa tgaaaggaca gatcaagaaa ggtacgaaat
     7081 tgatcttctg taccatagcc aagtccgatt tgagcattcc attggggagt ttcttcaatg
     7141 gttaatttta ctggaatgga atttcgagtg gaggaagcat caatctgagg ttggagcgag
     7201 gccaccctga aaagttgtaa atcgaaaagt tgttttcgtg ttttatccag tttcttattg
     7261 ctgtaaatct ctccttctgt gtaggcgagt tgacggcgaa tcaaactttc ttttatgttt
     7321 tgattgccaa caatttcggt cttcccaaaa aaacaaattt cgaatggtcg aataacataa
     7381 acgacatctg cccgatatga atcgagtgaa agattaatca aatgatcaac ttgtacatat
     7441 acatatcctg agttgataaa tatttgttcg atgcgggatt tgtcttcatg cacatctttg
     7501 tctctgaaac gcctgccgtt tttagataca atgtgttttt ttataagatg taatagtgta
     7561 tccttttttt cttccgaaat agattggctt tctatgtcga atccgatttc tccaaaaaat
     7621 atcggggtgt tttcttcgat tttaatataa atttcgactt tttgtttttt atgattggtg
     7681 gccaatgaat ccacttgcac ttcaacaaac gggaaacctt cggtttgata aaaacgggtc
     7741 attcgttcaa tatccaactc cattaaattt tcgttaaaaa ttaccggttc ctttttcaaa
     7801 aaagtacgtt gcaaaaaatt gtaagtagca aggttggttt tttccagtaa ttccgatttt
     7861 gaaaatgttt gattgccgac tatcgttatt ttttcaattt gatatccgtt ttgagctttc
     7921 gcggcaatgg cacaaaagta aaacagaaat aaaacgacag cttttccctg ccgaaaaata
     7981 ttaaattcca aaaatttgaa tcgatatggc attcctatgt cctaaaaatt taaacatcgg
     8041 ggttttgcag acgtgatgac aaaaaaatgc tgtttaattg gtaaaataat ttcatcagaa
     8101 tataatttat ttttt
//
LOCUS       DAOTAX010000069         8107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_7547_length_8107_cov_31.055514, whole genome shotgun sequence.
ACCESSION   DAOTAX010000069 DAOTAX010000000
VERSION     DAOTAX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7547_length_8107_cov_31.055514"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLM04_07160"
     CDS             <1..330
                     /locus_tag="PLM04_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON02888.1"
                     /translation="KNIQWLRPVVILTNRMTYSATNDFVNRMRKAPYAIILGDKTGGG
                     GGMPLSSEIPNGWMVRVPTSPMFDANMQHIEWGIDPDINIALKPEDAARGYDTLIERA
                     VALIKFL"
     gene            382..921
                     /locus_tag="PLM04_07165"
     CDS             382..921
                     /locus_tag="PLM04_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HON02889.1"
                     /translation="MQKLKVTEMNRLTVEEFKKKEKTPLIVVLDNVRSLHNVGSIFRT
                     ADAFLVQAVYLCGITSTPPNAELHKTALGAENSVEWKYFEETHKALEELRMMGYTIFA
                     IEQARESLPLNSIELNPANKYAIVLGNEVKGVQQTVVDACDGCIEILQYGTKHSLNVS
                     IAGGIVVWEFFKKLMEERN"
     gene            complement(1554..3308)
                     /gene="sppA"
                     /locus_tag="PLM04_07170"
     CDS             complement(1554..3308)
                     /gene="sppA"
                     /locus_tag="PLM04_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HON02890.1"
                     /translation="MKQFFKYTLATILGVILAGIILVAILFGIAGGIAGQKSAPTKLK
                     PNSVYELKLNGVLVERSEEDPFSIALAQAMGNEDNLTIGLNDILSNIQKAKTDENIAG
                     IYLNFGTLSAGYASMKEIRDALKDFKKSGKFVVAYADNYLQHEYYLASVADKIFLNPQ
                     GMLDWKGLASEITFYKNTLDKLGIEMQVVKVGTFKSAVEPYVNTKMSDANREQVTVYL
                     NSIWNTLLSEVSDSRKIPFEKLNVYADEMLTFQPTEKAKQYALVDSLVYQDEIDFIVK
                     GFLKDSTANINYVGHEAMCKVPDATKYDRNKIAVLYAAGAIDNGSDGIQSKKLVKTIN
                     EIAKDNSVKAVVFRVNSPGGSAYGSEQIWHALSQLKQKKPLIVSMGDYAASGGYYISC
                     IADSIVAQPNTITGSIGIFGLIPNIEGLDKKIGLTFDGVKTNKMSDAISINRPFRPEE
                     RDLMQNYVNRGYELFVKRCADGRKMSVEQIKAIAEGRVWTGKDAMEIGLVDKLGTIND
                     AISMAVKKAKLTSYQIKEFPKIETFSEKLLKELSDGTETRIEKLKLGEHYQMFKMIEQ
                     LDKMDGLQARLPYELTIR"
     gene            complement(3618..5117)
                     /locus_tag="PLM04_07175"
     CDS             complement(3618..5117)
                     /locus_tag="PLM04_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HON02891.1"
                     /translation="MQNKFKFVSAEEAATYVQHGDVVGFSGFTPAGCPKAVPVAIARR
                     AETFHAEGKEFKIGMYTGASTGVSVDGALAKADAVLFRTPYQSNKDMRNAINAGKAPY
                     FDLHLSTMAQELRFGFLPKPKFAILEVCDWNENGELVLTAAVGISPTIAEMADHIILE
                     WNHYHPKELKGFHDIYQPQDPPYRREIPIYKPSDRIGSEILKVDPAKILCIVETNLPD
                     EVSDFAPLDEVTQQIGNNVATFLAGEIRAGRLPHDFLPIQSGVGNIANALLFSLGKNE
                     EIPPFEMYTEVIQDAVIELMLEGNIKFASGCSLTVTDAVLQKIYQNIDFFRDKLVLRP
                     QEISNNPEMVRRLGLITINTALEADIFGNVNSTHVLGTKMMNGIGGSADFTRNSFISI
                     FTTPSTAKNGNISSIVPMVSHVDHTEHSVNVLITEYGIADLRGTTPKERAEIIIKNCV
                     APEYKQMLRDYLAISGSSHTPNNLKACFGMHIQLEKTGSMLGTNWDDFK"
     gene            complement(5639..6562)
                     /locus_tag="PLM04_07180"
     CDS             complement(5639..6562)
                     /locus_tag="PLM04_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HON02892.1"
                     /translation="MKILVATDKPFAKVAVDGIRAEVEAAGYELALLEKYTEKQQLLD
                     AVADADALIVRSDIVDAEVIKAAKKLKIVVRAGAGYDNIDLDAATQANICVMNTPGQN
                     SNAVAELVFGLLIYAVRNFYNGSSGTELKGKKLGIHAYGNVGRNVARIAKGFDMQIFA
                     YDAYCPAEVIKNDGVTPLTSVEELYASCDMVSIHIPATPETKKSINASLLQKLPKGAV
                     IVNTARKEVINEEDLINCMELRNDIKYVTDIMPDQHAVFAEKFPVRYFSTPKKMGAQT
                     AEANINAGIAAAKQIVSFFKTGDERFRVNKK"
     gene            complement(6651..7718)
                     /gene="serC"
                     /locus_tag="PLM04_07185"
     CDS             complement(6651..7718)
                     /gene="serC"
                     /locus_tag="PLM04_07185"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HON02893.1"
                     /translation="MKKHNFNAGPSILPREVIEKTAQAVLDFNGSGLSILEISHRSKD
                     FQAVMDEAIALFKELLEIPSGYSVVFLGGGASLQFCMVPFNLLEKKAAYLLTGTWASK
                     AYKEAKGFGEVIEVASSKDANFSYIPKNYVIPSDVDYFHITTNNTIFGTEILTDIDSP
                     VPLVADMSSDIFSRPVDVSKYGVIYGGAQKNLGPAGLAFAIVKEELLGKVSRHIPTML
                     DYRIHIENGSMFNTPPVLPIYSALETLRWLKANGGLEAMEKKNIAKAELLYNEIDRNK
                     LFVGTAAKEDRSRMNVCFVMNPEYQELEAEFLKFASDRGMVGIKGHRSVGGFRASIYN
                     ALHIESVQALVGCMQEFEKLH"
BASE COUNT         2564 a         1635 c         1467 g         2441 t
ORIGIN      
        1 aaaaatattc aatggttgag accggttgtg attcttacca accgcatgac ctacagtgct
       61 accaacgatt tcgtcaacag aatgagaaaa gcgccttatg ccattatttt aggcgacaaa
      121 acaggcggcg gcggcggaat gcccctttca agcgaaattc ccaacggttg gatggtcaga
      181 gtgcctactt cgcccatgtt cgacgccaac atgcaacata ttgaatgggg tattgaccct
      241 gatataaata tagccttaaa gccggaagat gccgcaagag gctacgatac actgattgag
      301 agagctgtag cgttgattaa atttttatga aaaatcgaaa attgaaataa ataatcgaaa
      361 ataaaatttt tgtttgagaa aatgcaaaag ctgaaagtta cggaaatgaa tcgcttgaca
      421 gtagaggaat tcaaaaaaaa agaaaaaacg ccgctgattg ttgttctcga taatgttcgc
      481 agtcttcaca atgtaggttc aattttccgc acagcagatg cttttctggt acaggcggtc
      541 tatttatgcg gaataaccag tacgccccct aatgccgaat tacacaaaac ggcattggga
      601 gcagaaaatt ccgtagaatg gaaatatttc gaagaaacgc ataaggcctt ggaagaattg
      661 cgaatgatgg gatatacaat ttttgccata gaacaggcac gtgaaagttt accattaaat
      721 tccattgaat tgaatccggc aaataaatat gccattgtat tggggaatga agtgaaaggc
      781 gttcaacaaa cggtagttga tgcctgcgat ggttgcattg aaatattaca atacggcacc
      841 aaacattccc tcaacgtaag cattgccggg ggcattgtag tttgggagtt ttttaaaaaa
      901 ttgatggaag aaagaaatta attccctttc agtttcttaa acgggagttt aacttactcc
      961 aaagatcttt cgaaattagt tggttttagg acgagggtat aaaatgaatg aggaaaatcg
     1021 ctgatagtaa caagaaatga agaaacctgt gcccaaacat cgcaacatgg attggctaat
     1081 atttagagag tccattgaaa agcactacca aatatcatac aaaaaggctc agatttataa
     1141 tttgctactt tataggtcaa gattttgaga aaaatacttc gcacatttaa aaaacaattc
     1201 aaagattatt ttgatagttg agaatgagta tcaccacgca aggcttctga aaaaattttt
     1261 gaactcaaac aaaaaattta catttttttg tttcgatact tactccatcc aatttcaatt
     1321 caaaaagccc ggtgattcat aagaaaatcg ataattcaca ttatgccttt agaatcgctg
     1381 aagttgagta aagcaaagca aagcttaatc agaatagcta ctcattttca aattccaaac
     1441 gaagaattat tgactatttg tgcaatttat tattaaacaa aatattaaaa agcaaaagag
     1501 gcaaaaccta tttttggttt cacctcttcc gaaaaacaaa ttggaactaa tatttatctg
     1561 attgtcaact cataaggcaa acgagcctga agtccatcca ttttatcgag ctgctctatc
     1621 attttaaaca tttgataatg ttctcccaat tttagttttt cgatgcgtgt ttccgttcca
     1681 tccgacaatt ctttcagtag tttttcgctg aaagtctcaa ttttaggaaa ttccttaatt
     1741 tggtaggaag tcagctttgc ttttttcact gccatagaaa tggcatcgtt tattgttccc
     1801 aatttgtcca ccagtccgat ttccattgca tcttttcctg tccaaacgcg tccttcagca
     1861 atggctttaa tttgttcaac cgacattttt cttccatcag cacaacgttt tacaaacaat
     1921 tcgtagcccc gattcacata attctgcatt aaatctcttt cttcgggtct gaagggacgg
     1981 ttaatactta ttgcatcgct cattttattg gtttttacac cgtcaaaagt taatcctatt
     2041 ttcttgtcta acccttcaat attggggata agcccaaaaa taccaataga acccgtaatg
     2101 gtatttggct gggctacaat gctgtcggct atgcaggaaa tataataacc acctgaagcg
     2161 gcataatcgc ccattgaaac gattaacggt ttcttttgct tcaactgact taaggcatgc
     2221 caaatttgtt cggagccgta agcacttcct cccggagaat ttactctgaa aacaactgct
     2281 tttactgaat tatcttttgc tatttcgttg atggttttca ccaatttttt tgactgaatt
     2341 ccgtcagatc cgttgtcaat tgcgccggct gcataaagaa ctgcaatttt gtttcgatca
     2401 tatttggtag catcgggaac tttacacatg gcttcatgcc caacataatt tatgttggca
     2461 gttgaatcct ttaaaaaacc tttgacaatg aagtcgattt catcctgata tacgagtgaa
     2521 tcgaccaaag catactgctt ggctttttct gtaggttgaa aagtcagcat ttcatccgca
     2581 tagacattta atttttcaaa aggaatttta cgactgtcgg aaacttccga gagcaatgta
     2641 ttccaaatgg aattgagata aacggtaacc tgttcgcggt tagcgtcgct cattttggtg
     2701 ttaacataag gttcaacggc cgatttaaaa gttccaactt tcactacctg catttcgatg
     2761 cccagcttgt caagcgtatt tttataaaaa gttatctcgg aggccaagcc tttccaatcg
     2821 agcataccct gtggattcag aaaaattttg tcggcaaccg aagcgaggta atattcgtgt
     2881 tgcagataat tgtcggcata agccacaaca aattttccgg atttcttaaa atctttcagc
     2941 gcatcccgta tttccttcat ggaagcatat ccggcagata aagtaccgaa attgaggtaa
     3001 ataccggcaa tattttcatc tgttttggct ttttgaatat tactgagaat atcgttcaaa
     3061 ccaatggtga ggttgtcttc atttcccatc gcttgtgcca aggcaatgga gaaagggtct
     3121 tcttccgaac gttctaccaa aactccattc aatttaagtt cataaacgga attaggtttt
     3181 aattttgttg gagcactttt ttgtccggct attccaccgg caattccaaa cagaatggct
     3241 accaaaataa taccggccaa aattacaccc aaaatagtgg caagtgtgta cttaaaaaat
     3301 tgcttcataa ataatgattg ttttgatatt aatatggcta agaaacaatt gataaaaaag
     3361 gagaaaaatc actgacaaaa atagagttta aagaaaaaat acaagctatt tctcaatgcc
     3421 gaatacgagt aaattaacaa gttcataaaa aactttacaa aggaaatcaa taattttctc
     3481 atttcaaacg attgaattca aatttttttt gaagcagcaa aattttctac cctttcagca
     3541 aaatgaaaaa gagaagcctt cttttaacaa aaaggttcct cttctcaaaa aaattatgaa
     3601 aaatctctcg aaatcaatta tttgaagtca tcccagttgg ttcccaacat gcttcccgtt
     3661 ttttcgagct gaatgtgcat gccgaaacag gctttcaaat tgttgggcgt gtgacttgat
     3721 ccggaaatgg caagataatc gcgcagcatt tgcttatatt caggtgcaac acagtttttg
     3781 ataatgattt cagctcgctc tttgggcgtt gttccacgta aatcggcaat cccatattca
     3841 gtaatcagca cattcacaga atgttcggta tgatccacat gagataccat cggaacaatg
     3901 ctgctgatat ttccgttttt ggcagtagaa ggagtggtaa atattgaaat aaacgagtta
     3961 cgtgtaaaat ccgcagaacc gccaatgcca ttcatcattt tagtgccaag aacgtgcgtg
     4021 ctgttgacat ttccgaaaat atcggcttca agcgccgtat tgattgtaat caaacccaac
     4081 cgacgcacca tttctggatt attggaaatt tcctgtggac gcagcaccaa tttatcccgg
     4141 aaaaaatcaa tattttgata aattttttgc aataccgcat cggtaactgt caacgagcaa
     4201 ccactggcaa attttatgtt gccttccagc atcaattcga tgacggcatc ctgtattact
     4261 tccgtgtaca tttcgaaggg tggtatctct tcatttttac ccaaagaaaa cagtaaagca
     4321 ttggcaatgt tacctactcc ggactgaata ggtaaaaaat catggggtag ccttccggca
     4381 cgaatttcac cagccagaaa agtagccaca ttgttgccaa tttgctgggt aacctcatcc
     4441 aatggcgcaa aatcggaaac ttcatcaggt aaattggttt caacaataca aagaattttc
     4501 gctggatcaa ctttcaaaat ttccgaacca attctgtcgg atggtttgta aatgggaatt
     4561 tctctgcgat aaggcggatc ctgaggttga taaatatcat gaaacccttt caactctttg
     4621 ggatgataat gattccattc cagaataata tgatcagcca tttcggcaat agtgggtgaa
     4681 atgcctacag cagctgttaa aaccagttcc ccgttttcat tccagtcgca aacttccaaa
     4741 atggcaaatt ttggctttgg caaaaatcca aaacgcaatt cctgagccat tgtggacaag
     4801 tgcaggtcaa aatagggagc tttcccagca ttgatggcgt tgcgcatgtc cttattcgac
     4861 tgataaggag ttctgaacaa cacagcatct gcttttgcca atgcaccatc taccgatact
     4921 cctgttgaag cgcctgtata catgcctatt ttaaattctt ttccttctgc atgaaaagtt
     4981 tctgcgcgcc tggctatagc tacgggaaca gctttggggc agcccgcagg cgtaaatccg
     5041 ctaaatccta ctacatcgcc gtgttgaaca taagttgcag cttcttctgc actgacaaac
     5101 ttaaatttat tttgcataaa aattggttta tttaatcttg aaaatccgcc gtaaaattaa
     5161 caaatatttc tcaatccttc aagaatttca gtgtcatggg cactaagttt acttttttat
     5221 aatttctcta agcacatgac aaaattttaa aatatcgata tttgattgtc aaaagttcca
     5281 gttcgttctg tcgatagcac tcgagtacgg tggatcatgc agtagaagca caaatatcaa
     5341 atgagcaatt aagtcttaaa gtttgtaatc agccataaaa gccgtattcg ccgcaatgaa
     5401 aaattgctgt cgcagctgtt ttgaagccaa aggcgagctt ttcaaaacaa acgacctgaa
     5461 accttacaaa cggcgcaaat aaagaggcta ataaacttta ttcgggctaa gtttatacct
     5521 ttaccgaagt gttgaaccaa aactgaagtt aaatcactac ttttggaata gaccaaagac
     5581 tttataataa aaaagtgtcc tttgaggaca ccatctggtc atttaaatca atacaagatt
     5641 attttttatt tacgcggaaa cgttcatctc ccgttttgaa gaacgacaca atttgcttgg
     5701 cggcagcaat gccggcatta atgttggctt cggcagtctg agcacccatt ttcttgggtg
     5761 tcgaaaaata acgtacggga aatttttcgg caaaaactgc atgctgatca ggcataatgt
     5821 cggtaacata tttaatatca ttacgcaact ccatgcaatt tatcaaatct tcttcgttaa
     5881 tcacttcctt gcgggccgta ttgactatga ctgcaccttt tggcaacttc tgaagcaaag
     5941 aagcattgat ggattttttt gtttcgggag ttgcggggat atgaatggaa accatatcgc
     6001 atgaagcata caactcttca acagaagtga gtggagtaac gccatcattt ttaataactt
     6061 cagcgggaca ataagcatca tatgcaaaaa tttgcatatc gaaacctttt gctattctgg
     6121 ccacatttct gccaacattt ccgtaagcat gaataccaag ttttttccct ttcaactctg
     6181 ttcctgaaga cccgttataa aaattacgca cagcataaat caacagtccg aaaaccaact
     6241 cggcaactgc attagaattt tgccccggcg tattcatgac acaaatattt gcctgggtag
     6301 ccgcatcaag gtcaatatta tcataaccgg ctccggcccg aacaacaatt ttcaattttt
     6361 tggctgcctt tatcacttca gcatctacaa tatcactgcg aacaatcaaa gcatcggcat
     6421 cggccacggc atcaagcaac tgttgctttt cggtatattt ttcgagcagg gcaagttcgt
     6481 atcctgcggc ttccacttct gcccgaattc catccactgc cactttggca aatggtttat
     6541 cggtagctac aagtattttc ataagcaaac ttttttctct cctttttcaa taaagaagaa
     6601 ttatttatgt taatggttaa aattgtaaac agctgttgct accaaaagca tcaatgcaat
     6661 ttttcaaatt cctgcatgca gccaaccaat gcctgaacgc tttcgatatg aagtgcattg
     6721 taaatggaag cgcggaaacc gccaacagaa cgatgccctt taattcctac cataccacga
     6781 tcggaagcaa atttaagaaa ttcggcttcc agttcctgat attcaggatt cataacgaaa
     6841 caaacattca tgcgtgaacg atcctctttg gctgccgttc caacaaacaa tttattgcgg
     6901 tcgatttcat tgtagagcaa ttcggctttt gcaatatttt tcttttccat tgcttccaaa
     6961 ccaccatttg ccttcaacca gcgcaacgtc tcaagtgccg agtaaatggg aagaaccggc
     7021 ggcgtattga acatcgaacc attttctata tgaattctgt aatcgagcat agtggggata
     7081 tgacgagaaa ctttgcctag aagttcttct tttacaatgg caaaagccaa acctgcaggt
     7141 cccaaatttt tctgtgcacc tccataaata acgccatatt ttgaaacatc tacaggacgt
     7201 gaaaaaatat cggacgacat atcggcaacc aaaggcaccg gagaatcgat gtcggtcaaa
     7261 atttcggttc cgaaaattgt attattggtt gtaatatgaa aataatcgac gtctgagggg
     7321 atcacataat tttttggaat atagctgaaa ttggcatctt tggaggaagc aacttctatt
     7381 acttctccga aacctttagc ttccttgtaa gccttgctgg cccaagtacc ggtaagcaaa
     7441 taagcagctt tcttttcaag caggttgaag ggaaccatgc aaaattgcaa acttgcgcct
     7501 ccacccaaaa aaactaccga ataacccgaa ggaatttcca acaattcttt aaaaagagca
     7561 atggcttcat ccattactgc ctgaaaatcc ttagaacgat ggctaatttc caaaatcgac
     7621 aatccggaac cattgaaatc caataccgct tgtgctgttt tctcaatcac ttcacgaggt
     7681 aatatcgatg gccccgcatt aaaattatgc tttttcatca tttcattaaa aaattttttt
     7741 ggtttataag tttctccctt tgaatttaaa gcacaaaagt acacaaattt taaattcttt
     7801 catttcttat ggaaaaattt tttagtttca attttaaatt ttcatatagg agtctcctct
     7861 gaaaagttac tcaaagagaa ttagtttttt aaaaacattg agataaaaaa ggtaaaaaat
     7921 attcaaaaaa gttctttgaa atttgcatat attgttatat ttcagtatct taggaggttg
     7981 aaatacgata aaaagagaga taaatgttca ggaaaacaga aagcgaaata caattagata
     8041 tatttttttc gcccgaaaaa cttatcccac attacaggta cttagctcca taatacctgc
     8101 aatgtgg
//
LOCUS       DAOTAX010000070         7925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_7785_length_7925_cov_26.849301, whole genome shotgun sequence.
ACCESSION   DAOTAX010000070 DAOTAX010000000
VERSION     DAOTAX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_7925_cov_26.849301"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1193)
                     /locus_tag="PLM04_07190"
     CDS             complement(144..1193)
                     /locus_tag="PLM04_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HON02894.1"
                     /translation="MLIKEHYPLTGKPYPKRLIFKVVGYSSSTWYENPTPRTGKPGRK
                     PKHTDEEVLREIKTEIKKSSFNAEGYMKVKKRMGKREVNPLVAGKARVNRIMRENNLL
                     SPNRRTGKINKREHDGIIITNAPNLMWATDGKKFWIDGLGWHWFFGVIDHFNDEIISW
                     HIAKKGNRFAAMEPIRAAVRKTFGSVDKDVCKGMKLQLRSDHGSQYDSADFMNEMKFL
                     GLEMSKAFVRSPQCNGIIERFHRTLDEQVLQTETFSSFQEAYNSINRFIKNYNTDWIF
                     HRLDYCSPVEYREQYAQSRRKEKNDIPSGNKEPEVQLVLISSGSMPRRNELVNQGMIK
                     GAITCSNTPSINPSV"
     gene            complement(1199..1480)
                     /locus_tag="PLM04_07195"
     CDS             complement(1199..1480)
                     /locus_tag="PLM04_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02895.1"
                     /translation="MEKRKNLTSKIKAEIVLSLLRGEDPELISREYGVTLSEINHWRD
                     QFIESGTEGFKRKPEDSRLSAAERKIGQLQMELELTKKKNELAVKLRKK"
     gene            complement(1440..2687)
                     /locus_tag="PLM04_07200"
     CDS             complement(1440..2687)
                     /locus_tag="PLM04_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02896.1"
                     /translation="MKKILIAILMLIVLMPLIMWLLWIIKKPRELNILILDKTVLTSA
                     VQEHLSLSWVINNEKFIHNSKSYNKQKDYYGFFPDDKGNYHIRDFENFSSENLDNLAN
                     SYDGAYYTDMYGIYTNEWIIRYYPEKIHDKRFIADRSKKIYGGMTQKELDFLRLMKQK
                     NKLIINEFNVIASPTSKEIRNTYEKEFNIHWTGWVGRYFDNLDTLVNEDIPRWLIDNY
                     TRNHSGQWPFKKSGIAFVSENDQILILEKDTHLNTDIPYIYTNENNAKHYHLPEKIKY
                     PFWFDIMETNPSFRIISSYHLDTNAAGDSLLALGRIPKIFPAAVNYKNGYNYYYFCGD
                     FADNPISYASSRFRYIQVFSPLFYSSSENERASFFWKYYRPLLRQILNEYISPQKNRA
                     VKNKSYFCNCYGKEKKFDIKNQG"
     gene            complement(2945..>4311)
                     /locus_tag="PLM04_07205"
     CDS             complement(2945..>4311)
                     /locus_tag="PLM04_07205"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HON02897.1"
                     /translation="LQYFKEDFESYEAGSGKTIPASAGNFFILNKDFLGTRTWECRIF
                     SNNKYAQVTSNASNTENQVWLITKQIDLTDANVPSLTFDVNVGYYNADCLSILISENF
                     DGTEAGVASATWTDVTSSFTLPQTPTSGYGTLASAGTLELSAFKGKKINVAFKYTGDG
                     RTDANPKKTTTYQVDNVVVSDTKTVSTVSDPKTQFSYYIYQNGQWNFVNASFYQLAEE
                     DYTSMGVTTLNATTAPNYLPILLKIKFPFAQEGATKVVVYKTSATKNAADEYIYKSGV
                     WSPISLIETRTEQFVFAGWENGGWIFDPTLRVTMKKGTNPTDDYMLVVNYVRDTYGAQ
                     TPSLLGYYGTSLQSEYYYGFAAYYGNISWRESDRAKDPTYAALTTAEEKQNYLVERTK
                     EGLAVYLSLKFPDAQPKVSGVDLYCYVTTAIYNGVTTTNYIYKYQRVDGEPFGWKYIE
                     SNPL"
     assembly_gap    4312..4411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4412..5016)
                     /locus_tag="PLM04_07210"
     CDS             complement(<4412..5016)
                     /locus_tag="PLM04_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02898.1"
                     /translation="MKKLLIFILSSIMIALTFSSCDYNEENFPGLDEMAAYKDVVNYE
                     YTISESDFTTIISALRANKNAQDSATATLLSSSKIFSVSAPASTLVPYVLKSKFYGAT
                     SGSAAKVTYQYKTGSYQYYLLTQADYKTIWQQDFIYALTPSKSPQSVLPGFLSQKFPA
                     AKEGDIKILEYSYSSADPVTSEVPLQYFKEDFESYEAGSGKT"
     gene            complement(5119..7785)
                     /locus_tag="PLM04_07215"
     CDS             complement(5119..7785)
                     /locus_tag="PLM04_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02899.1"
                     /translation="MRKVFSFLFMMMLAQFAFSQASVKGVVVDKSTNEPLVGVSVLQP
                     ALGIGTTTSLDGSFSLRLPEGKHELRFSYVGYSTKIVQVSSNNVNLGFVQMESEAIGL
                     NDVTVTSSLAIRRKTPVALSVIEPLVIEEKLGTQEFPEILKSTPGVYATKQGGGFGDS
                     RINLRGFESANIAVMINGVPMNDMEWGGVYWSNWAGLSDVTRSMQVQRGLGASKVAAP
                     SVGGSINIVTKSTDAKKGGSFSYSIGNDGYNKISFNVSTGLSETGWAMTFLGAKTWGN
                     GYIQGTEFEAYSYFINISKIINEDHQISFTGFGAPQWHNQRYNGDKLLIPEWQKLKDG
                     YKFNPTYGFDENGQRKTGNYNHYHKPQLSLNHYWNMNDKSSLSSALYLSIGNGGGYGW
                     RGNTGTLMYGTNTATGLLNTTYRTLDGYMDYGKLQAENAANLNGSQAVIADSRNNHTW
                     LGLLSTFTTKFGQNIDFYGGLDLRYYAGMHDAVLVDLMGGKFFIDPARVNVKNSVNAG
                     NPAYVNEKLQVGDIVYRDNTGYVVQEGVFSQAEYSKGKLNVFLAGSLSNSTYWKVDRF
                     YYDNQKSDSKSFIGFTAKTGANYNLTDKHNVFANIGYISRAPFMSGGYFVTIHTSNAV
                     NKNAVNEKVFSTELGYGYRSKFLTANVNLYRTAWMDKTMVRSFGSNSDDGVVNLKGVN
                     ALHQGLEIDLVSKPVKNLELTGMLSLGDWEWTSNATGYVFNKDGQPVNASGSTVDKNG
                     NTIQMFSADHAFVNLNLKGVKVGNSAQTTFALGAKYNFLKDFSAGLDYTHYARNYANF
                     SITANVGTTNYTTPWQIPAAGVFDFNANYKFKLQGFDASLNGVINNLFNQVYITDAQD
                     LTPTTSASDEWKNTAVLYGFGRTYSISLRVRF"
BASE COUNT         2298 a         1593 c         1455 g         2479 t
ORIGIN      
        1 gaattttaaa aattgtgttt attctgttcg ctgatattct tcgtttaaaa tttgtgtatt
       61 agcccgaaat aaccgagcac attaattgta tttaatactt tttattattt ttgtatcgga
      121 caagctgatc cgatggtgat attttatacc gaaggattaa ttgaaggggt gttgctgcaa
      181 gtaatagccc ctttaatcat tccttgattg accaattcat ttcttcgagg cattgagcca
      241 cttgaaatga ggacaagctg aacctcaggc tccttattgc ccgatggaat atcatttttt
      301 tcttttcgcc ggctttgtgc gtactgttct ctgtattcta ccggagaaca gtaatctaat
      361 ctatgaaata tccaatctgt gttatagttt ttaataaatc gattaatact gttataagct
      421 tcctggaagg aagaaaatgt ttctgtttgc agcacctgtt cgtccaatgt gcggtgaaac
      481 cgctctatta tcccgttgca ttgtggagag cgtacaaatg ctttggacat ttccaatccc
      541 agaaatttca tttcattcat aaaatcggca gagtcatatt gtgacccatg gtcgcttctt
      601 aattgcagtt tcattccttt gcaaacatcc ttatctaccg aaccgaaagt ctttcttacg
      661 gcagctctaa tgggctccat agctgcaaaa cgattgcctt ttttggctat atgccatgag
      721 ataatctcat cattaaaatg atcgatcaca ccaaaaaacc aatgccaccc taacccgtca
      781 atccaaaact tcttaccgtc agtggcccac attagattgg gtgcattcgt gatgatgata
      841 ccgtcatgtt cacgtttatt gatctttcct gtccttctgt tggggctgag caggttgttt
      901 tcccgcatga tgcggttcac acgtgcctta cccgctacaa gagggtttac ctctcttttc
      961 cccatgcgtt tcttcacttt catataacct tcagcgttga aactgctctt cttgatttca
     1021 gttttaatct cccgtaaaac ctcttcatcg gtgtgcttgg gctttctgcc gggcttgccg
     1081 gttcggggtg taggattttc ataccaggtg ctgctactgt aacccactac tttgaatatc
     1141 aaacgcttcg gatatggctt ccctgttaat ggataatgtt cctttatcag cattaagatc
     1201 atttctttct tagttttacc gctaattcgt tctttttttt tgtgagttca agctccatct
     1261 gtaattgccc tatcttgcgc tcggcagcac tgagccttga atcctcaggt ttacgcttaa
     1321 acccctctgt accactctcg ataaattggt cgcgccaatg attaatctcg gacaaagtaa
     1381 ctccgtattc tcggctgatc agctccggat cttcccctcg taacaaggat aaaacaattt
     1441 cagccttgat ttttgatgtc aaattttttc tcttttccat agcagttgca aaaataactt
     1501 ttatttttta ctgctcggtt tttttgaggg ctaatatatt cgtttaaaat ttgtcgaagc
     1561 aggggacgat aatatttcca aaaaaaactt gctctttcat tttcggaaga agaataaaaa
     1621 agtggtgaaa acacttggat ataacggaaa cgcgaacttg cataagaaat gggattgtcg
     1681 gcaaaatcgc cacagaaata ataataattg tagccgtttt tataattcac tgcagccgga
     1741 aatattttgg gtattctgcc aagcgccaac agggaatcgc cggcagcatt ggtatcgaga
     1801 tgataagaag aaataattcg aaaggaagga tttgtctcca taatatcgaa ccagaaagga
     1861 tatttgatct tctcgggaag atgataatgt tttgcattat tttcattggt gtaaatgtaa
     1921 ggaatgtcgg tattcaaatg ggtatctttt tccagaatca gtatttggtc attttcactg
     1981 acaaaagcga ttcccgattt tttaaaaggc cattgtccgc tgtggttgcg ggtgtagttg
     2041 tcgatcagcc agcggggtat atcctcattc acgagggtgt cgagattgtc gaaatagcgt
     2101 cccacccaac cggtccaatg aatattaaat tctttttcgt atgtgttgcg tatttctttc
     2161 gaggtaggag aggctatcac attgaattca ttaatgataa gtttattttt ttgtttcatc
     2221 aatcgaagga aatcaagttc tttttgtgtc atgccaccgt aaattttttt ggaacgatcg
     2281 gcaataaatc ttttatcgtg aattttttcg ggataatacc ggataatcca ttcattcgtg
     2341 taaataccgt acatatctgt atagtaagca ccgtcgtagc tatttgccaa attgtccaga
     2401 ttctcagagg aaaaattttc aaaatctcga atatgataat ttcccttatc gtcagggaaa
     2461 aaaccgtaat agtctttttg tttgttgtaa gatttgctgt tgtgaataaa tttttcgttg
     2521 ttgattaccc acgaaagcga caaatgttcc tgtacggcag aagtcaatac ggttttatcc
     2581 aaaatcagaa tattcagctc tctcggtttt ttaattatcc acaaaagcca cattatgaga
     2641 ggcattagta caatgagcat cagaatcgca atcagtattt ttttcattca tttatttaat
     2701 ttttttcaat agtttaacaa atagaaaaaa taaaagtttt actttttagc caatttattg
     2761 aaaatatttt ttaatttttt cgaacattgg tatcagcatg ggatagattt atatggcaac
     2821 tgtttttggt acaaagttat ttttttagaa tggatgaaca agggcaagct gcaaatatgc
     2881 ttaaccaaaa agccggcaga taaacgccgg ctctttgaaa aaacactaaa aagtagaaga
     2941 aaaattataa aggattgctt tcaatgtatt tccacccaaa tggttctcca tcaacacgct
     3001 gatacttgta aatataattt gttgtggtaa cgccgttgta aatagcagtg gttacataac
     3061 aatacaaatc aacgccgctg acttttggct gagcatcggg aaatttcaaa ctcagataaa
     3121 cggcaagtcc ctctttggtg cgctcaacaa ggtaattctg tttctcttct gcagtggtta
     3181 gtgctgcata agtcggatct ttagctcggt cggattcccg ccatgaaata ttgccgtaat
     3241 aagccgcaaa gccataataa tattccgatt gcagactggt tccatagtat ccgagcaatg
     3301 atggagtttg tgctccatat gtatctctta catagtttac aaccaacata tagtcatccg
     3361 tcggattggt gccttttttc atggttacac gcaatgttgg gtcaaaaatc caaccaccat
     3421 tttcccaacc tgcaaataca aattgttccg ttctggtttc aatcagcgaa ataggcgacc
     3481 aaactcccga cttgtaaata tattcatcgg ctgcattttt tgtggcactt gttttgtaaa
     3541 ccaccacttt cgtagctcct tcctgtgcaa acgggaattt gattttgagc aatatcggca
     3601 aataatttgg agctgtagta gcgttcaaag tagtaacacc cattgaagta taatcttctt
     3661 cagccaattg gtagaaagaa gcatttacaa aattccactg tccgttttga taaatataat
     3721 atgaaaattg agtttttggg tctgaaacag tgcttaccgt tttggtatcc gaaacaacga
     3781 cattatcgac ctgataagtg gtggtttttt tcggattggc atctgttcgc ccatctccgg
     3841 tatatttgaa tgccacatta atctttttcc ctttaaacgc agaaagctca agcgtaccgg
     3901 cagaagctaa cgtaccataa ccggaagttg gagtttgtgg aagagtaaaa cttgatgtaa
     3961 catctgtcca tgtagctgaa gcaacaccgg cttccgtgcc atcaaaattt tccgaaatca
     4021 gaatactcag acaatctgca ttatagtaac caacattaac gtcaaaagtt aacgacggaa
     4081 cattggcatc cgtcaagtca atttgcttgg ttatcagcca aacctgattt tcggtgtttg
     4141 aagcattcga ggttacctgt gcatatttat tgtttgaaaa tatacgacat tcccacgttc
     4201 ttgttcccag aaagtccttg ttcagaataa aaaaattgcc ggcagaagca ggaatcgttt
     4261 ttccagaacc agcttcgtaa gattcaaaat cttctttaaa gtactgaagc gnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtttttcca gaaccagctt cgtaagattc
     4441 aaaatcttct ttaaagtact gaagcggcac ttcagatgtt acagggtcgg cagaagaata
     4501 ggaatattcc aaaattttaa tatcgccttc ctttgctgca gggaactttt gcgataaaaa
     4561 tcccggcaac acactttgtg gagatttgga aggtgtaagt gcgtaaatga aatcctgctg
     4621 ccaaatggtt ttataatcag cctgggtaag cagataatac tgatacgaac ctgttttata
     4681 ctgataagta acttttgcag cagaaccgct tgtagcgcca taaaatttgc ttttcaacac
     4741 ataaggcact aaagtggaag caggagcgct tacagagaaa attttgctac tgctcaataa
     4801 agtggctgtg gcagaatctt gggcattttt attggctcgc aatgccgaaa tgatggtggt
     4861 aaagtcggat tcggaaatcg tatattcgta attgactaca tctttatagg ctgccatttc
     4921 atccaacccg ggaaaatttt cctcattata atcgcacgac gaaaaggtca atgcaatcat
     4981 tatggatgac aggataaaaa ttaaaagctt tttcatacgt cgtaatttta atttatttgt
     5041 aaaacttgct gaaaaatacc tgaaaatcca gtttttttct tgagttgttt tatcttaatg
     5101 actaaaaatt atttttactt aaaatcgaac tcgcaaactg atagaatagg ttcttccaaa
     5161 accatacaac acagcggtat ttttccattc atcggaagca gaagttgtag gggtcaagtc
     5221 ctgtgcatca gtaatatata cctgattgaa caaattattg atcactccat tcaaggaagc
     5281 atcgaatccc tgaagtttaa acttgtaatt ggcattaaag tcaaaaactc ctgcagcagg
     5341 aatttgccac ggcgtggtat aattggttgt tccaacatta gccgtaatgc taaaattggc
     5401 atagttgcgg gcataatgcg tataatccaa tcctgctgaa aaatctttca ggaaattata
     5461 ctttgcaccc aaagcaaaag tggtttgagc cgagttacca actttgacac ctttcaaatt
     5521 caaattgaca aatgcgtgat cggccgaaaa catttgaatg gtatttccat ttttatccac
     5581 tgtcgaacca cttgcattga ccggctgtcc atctttgtta aacacatatc ccgtagcgtt
     5641 gctggtccat tcccagtctc caagcgacag cattccggta agttcaagat tcttaacagg
     5701 cttacttaca aggtcaattt ccaaaccctg atgaagagca tttacacctt tcagattgac
     5761 aacaccatca tccgaattgg agccgaaaga acgaaccata gttttatcca tccaagccgt
     5821 acgataaaga tttacatttg ccgttaaaaa ttttgaacga taaccgtatc ccaattcagt
     5881 tgaaaaaact ttttcgttga ctgcattttt atttacggca ttgctggtat gaatagttac
     5941 aaaataaccg cccgacatga acggagcgcg tgaaatataa ccgatattgg caaaaacatt
     6001 gtgtttgtcg gtaaggttat agttggcacc ggttttggcg gtaaaaccga taaagctttt
     6061 tgaatccgat ttttggttgt cgtaataaaa acggtccact ttccagtaag tgctgttcga
     6121 caaagaaccg gccaagaaca cattcaattt tcctttggaa tattcggcct gcgaaaaaac
     6181 gccttcctgg accacataac ccgtattgtc gcgataaacg atatctccta cttgcagttt
     6241 ttcgttgacg tatgcaggat taccggcatt caccgagttt ttaacattca ctcttgcagg
     6301 atcgataaag aattttccac ccatcaaatc cactaataca gcatcgtgca ttccggcgta
     6361 gtaacgcaag tcgagtcctc cgtaaaaatc tatattttgt ccgaatttgg tagtaaaagt
     6421 tgaaagcaaa cccagccagg tatggttatt tctcgaatcg gctattacgg cttgtgaacc
     6481 attcagattg gctgcatttt ctgcctgtaa ttttccataa tccatataac catccaaagt
     6541 acggtaagtg gtattcaaca aaccggttgc cgtattggtt ccatacatca aggtgcctgt
     6601 gtttcctctc caaccataac cgcctccatt acctatggaa agatacaaag ccgaagaaag
     6661 actcgatttg tcattcatgt tccaataatg attcaatgac agttgaggtt tatgataatg
     6721 gttgtaattc cccgtttttc tctgtccgtt ttcatcaaaa ccataagtag gattaaattt
     6781 ataaccgtct ttgagttttt gccattcagg tatcagcagc ttgtcgccgt tgtatcgctg
     6841 attgtgccat tgaggcgctc caaaaccggt aaaagaaatc tgatgatctt catttataat
     6901 ttttgaaata tttataaaat aggaataggc ttcaaactcg gttccttgaa tgtaaccatt
     6961 tccccatgtt tttgctccca gaaaagtcat tgcccagccc gtttcggaaa gtccggtgga
     7021 gacattgaac gaaattttat tgtagccatc attcccaatg gaataggaaa aagagccacc
     7081 tttttttgca tcggtcgatt tggtaactat gttgatggaa cctcctaccg aaggagctgc
     7141 aactttggaa gcgcccaaac ctctttgaac ctgcatagaa cgggtaacat cgcttagtcc
     7201 ggcccaattc gaccagtaaa cgcctcccca ctccatgtca ttcatgggaa ctccattgat
     7261 catgacggcg atattggccg attcaaaacc acgtaaattg atacgtgaat cgccaaaacc
     7321 accaccttgt ttggttgcat atacacccgg agtggatttt aatatttcag gaaattcctg
     7381 cgtacccagc ttttcttcaa ttaccaaagg ctctattact gacagagcaa caggcgtttt
     7441 acggcgaatt gccaacgaag aagtaacagt tacgtcattc aaaccaatgg cctccgactc
     7501 catttgtaca aatcccaaat tgacattgtt cgaagaaact tgaacaatct ttgtagaata
     7561 gcctacataa gaaaacctaa gttcgtgttt tccttccggc aatctcagag aaaaacttcc
     7621 atcaagcgaa gttgttgtcc caattcccaa agcaggttga agaacactta ctccaaccaa
     7681 aggttcattg gtagatttat ccactaccac ccctttaact gaagcttgcg aaaatgcaaa
     7741 ctgcgcaagc atcatcataa atagaaatga aaatactttc ctcataagaa ttctcttttt
     7801 tagtttttaa ttttttaaga taagcatggc aaatatattc tattttttta atatcacata
     7861 tcattaaaac cgtaaattca agtaattaat gcaacttttt caggggaaat aaaaggtaaa
     7921 gaaaa
//
LOCUS       DAOTAX010000071         7759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8026_length_7759_cov_31.818536, whole genome shotgun sequence.
ACCESSION   DAOTAX010000071 DAOTAX010000000
VERSION     DAOTAX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_7759_cov_31.818536"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PLM04_07220"
     CDS             complement(<1..167)
                     /locus_tag="PLM04_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HON02900.1"
                     /translation="MNKIVNIALQILPSSATIHPYKLVDKAIEVIAASGLKYRVTPFE
                     TVMEGTYDEVM"
     gene            complement(359..892)
                     /locus_tag="PLM04_07225"
     CDS             complement(359..892)
                     /locus_tag="PLM04_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HON02901.1"
                     /translation="MPVKIRLQRHGHKGYAFYHIVIADSRTPRDGRFIERIGSYNPNT
                     DPSSVDLKFDRALYWLQVGAQPTDTVRNILSREGVLMKKHLLEGVKKGAFDEAEAERR
                     FEAWKSSKEAKLNKTKEQLTAEREAAEKARLSAEIEANKARAAELAKKKAAEEAKTKE
                     KDNSSENSEEQEPKEVE"
     gene            complement(1000..1950)
                     /locus_tag="PLM04_07230"
     CDS             complement(1000..1950)
                     /locus_tag="PLM04_07230"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02902.1"
                     /translation="MEKLSRYIEILLAQHDYVIVPGLGGFIIQPQSAMILSDRITPPL
                     AVIAFNPLLNSNDGLLAYEISEREHISYRAAIEMIDHEVESILSKLQQGEKVDMENLG
                     SLQKNTNGHLIFSPVPRADFLPANFGMTEIKLWKRRITDSEERKKITITLPSASSFRY
                     AAACALIFGMLLVSPRMADTKRSHYANLIPLETYTPTQSQPSASKNVSINNTLKATVE
                     TEKQDSLSPAKEENFQNALSKQTNLYHVIAATFYSRKSAEKFCAQLQNLKYTEARVFS
                     PEKNYKVVVRSFPRYQEAVEFMEQLRKSDALFESAWVYCK"
     gene            2069..3133
                     /gene="rfbB"
                     /locus_tag="PLM04_07235"
     CDS             2069..3133
                     /gene="rfbB"
                     /locus_tag="PLM04_07235"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HON02903.1"
                     /translation="MEKTILITGGAGFIGSHVVRLFVNKYPDYHIVNLDALTYAGNLE
                     NLKDVADAPNYTFVKGDITDEEFIPQLFEKYDFDGVIHLAAESHVDRSISDPLAFIRT
                     NVFGTANLLNAAKEAWKGKMEGKRFYHISTDEVYGSLGKEGYFTEKTPYDPRSPYSAS
                     KASADHFVRAYYHTYGLPVLISNCSNNYGSHHFPEKLIPLCINNIRYNRPLPVYGKGE
                     NVRDWLWVDDHAAAIDIIFHQGEIGETYNIGGHNEWTNIRLIEKLCEIMDKKLGRESG
                     ESAKLITFVKDRAGHDLRYAIDSSKLQRELGWQPSITFEEGLEKTVDWYLENQEWLDN
                     IINGDYQNYYEKQYSEREKS"
     gene            complement(3190..4311)
                     /locus_tag="PLM04_07240"
     CDS             complement(3190..4311)
                     /locus_tag="PLM04_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HON02904.1"
                     /translation="MKALKTKLKINYQSFGLKRIDTYIINKFLGTYFFCIVLILSISV
                     VFDLTEKLDNFFETNAPLKAIIFDYYLNFIPYYMNMFSPLFTFIAVIFFTSKMANNTE
                     IIAILSSGVSFRRLMLPYFISATLIALLFFFLGGYVIPNSSKKMLNFENQYVKKFKPS
                     NARNVQMEVEKGVIMYIERYEVDNNIGYHFSLEKFDGKKLVSRLTAETITWDSAYNWK
                     INNYLQRDFIGMREKITKGISMDTVIKVQPKELFITSEEAAQMTNSELRQYLHRQRAR
                     GVGNIQSFENEYYRRYAMPFAAFIMTLIGVSLSSRKVRGGMGLNLGIGLALSSIYILF
                     STLSTTFSVNGTLSPFMAVWLPNIVFLLIGIYLYQTAPK"
     gene            complement(4339..5469)
                     /gene="tgt"
                     /locus_tag="PLM04_07245"
     CDS             complement(4339..5469)
                     /gene="tgt"
                     /locus_tag="PLM04_07245"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HON02905.1"
                     /translation="MNFEILYTDSGSKARAGKITTDHGAIETPIFMPVGTAGSVKTVH
                     FRELEEDIHAQIILGNTYHLYLRPGTDILEQAGGLHKFIGWDHPILTDSGGFQVFSLT
                     GIRKLKEEGVLFQSHIDGSKHFFTPEITVDIQRTIGADIIMALDECTPGTADYQYAKK
                     SMQLTHRWLLRGIEHFDTVSPKYGYSQAFFPIVQGCVYPDLRRESAKFIADQNKDGNA
                     IGGLAVGEPTEKMYEMIEIVNDILPENKPRYLMGVGTPQNILESIERGVDMFDCVMPT
                     RNGRNGMLFTSQGVINIKNAKWKNDFSPLDEKGTSFVDKAYSKAYVRHLFAANELLGL
                     QIASIHNLAFYLWLMNEARLHIRKGDFSLWKKGMIEQLGRRL"
     gene            complement(5496..6086)
                     /gene="hisH"
                     /locus_tag="PLM04_07250"
     CDS             complement(5496..6086)
                     /gene="hisH"
                     /locus_tag="PLM04_07250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HON02906.1"
                     /translation="MKVALVKYNAGNIRSVLFALERLGIEAVVTDDAEEILSADKVIF
                     PGVGEASTAMNYLKHNRLDLLIPSLKQPVLGICLGMQLMCSFSEENNTTCLGIFDEKV
                     KHFPANENFKVPQMGWNNIFRLSGPLFDGIKKNSFVYFVHSYYVPLSSNTAAVANYIF
                     DFSAALQKNNFFAVQFHPEKSGEIGQKMLENFLNIC"
     gene            complement(6102..6455)
                     /locus_tag="PLM04_07255"
     CDS             complement(6102..6455)
                     /locus_tag="PLM04_07255"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HON02907.1"
                     /translation="MQIEILKSKIHRVSVTDANLNYIGSITLDQDLMDAANIIPYEKV
                     SVVNNNNGERIETYVIPGERGSGMVCLNGAAARKVQIGDVVIIMAFATMDFEEAKKFT
                     PVVIYPDTETNQLKK"
     gene            complement(6503..7357)
                     /gene="panC"
                     /locus_tag="PLM04_07260"
     CDS             complement(6503..7357)
                     /gene="panC"
                     /locus_tag="PLM04_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HON02908.1"
                     /translation="MKVFSTKKELQKEMEQIRKEGKTVGFVPTMGALHEGHLSLINRS
                     VAENDVTAVSIFVNPTQFNNRSDLEKYPRDLQRDLLLLEKNGCDIVFSPSVDEMYDKN
                     EFESVFEFDFGGLDQVMEGKFRPGHFNGVVQVVSKLFNLVQPTKAYFGEKDFQQLAII
                     RRMVNLMNFDIEIVGCPIVREKDGLAMSSRNLRLNAEERQKAVEISKTLFESKKLISN
                     FSPEQVQNWVIARLNSFSGLEVEYFEIVHQTTLQPIHSWSEPAIGCIAVFCGEVRLID
                     NIKYNDLQ"
     gene            7669..7742
                     /locus_tag="PLM04_07265"
     tRNA            7669..7742
                     /locus_tag="PLM04_07265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7703..7705,aa:Arg,seq:acg)
BASE COUNT         2278 a         1532 c         1495 g         2454 t
ORIGIN      
        1 tccatcactt cgtcataagt tccttccatc accgtttcaa aaggagttac cctgtatttc
       61 agacctgaag cagcaatcac ttctattgcc ttatccacca gtttatatgg atgaatggta
      121 gctgaactgg gtaaaatttg aagtgcaata ttgacaattt tattcatttt tacgcagatt
      181 aaatattgac aactacttat aaattgaaac gtataaacag tttaaaaatt gtagcttttt
      241 gcaatttatg ttctaaaaat aacatcggaa aattcttctc tgtgataaaa caaaaaagcc
      301 caaaaatact tccgggcttt ttttagagca agtcaaattt gaatgttttt aagaggattt
      361 attctacttc ttttggttct tgttcctcag agttttctga agaattgtct ttctcttttg
      421 tttttgcttc ttcagcggct tttttctttg ccagttcggc agctctggct ttatttgctt
      481 ctatttctgc cgacagacga gctttttcgg cagcttctct ttcagcagta agctgttctt
      541 tggttttgtt caatttagct tcttttgaag atttccatgc ttcaaagcgg cgttcggctt
      601 ctgcttcgtc gaaagcacct ttttttacac cttccagcaa gtgttttttc attaatacgc
      661 cttctcttga taaaatgttg cgtaccgtgt cggtgggttg tgcacctacc tgaagccaat
      721 acaaagctct gtcaaatttt aagtctactg aacttggatc ggtgttggga ttataggaac
      781 caattctttc gataaatctg ccatctcgtg gcgtacgact gtcggcaata acgatatgat
      841 aaaaagcata tcctttgtgt ccatgtcgtt gtaatctgat ttttacgggc atttttaaaa
      901 tttttttatt gttaatacct aattgctttt tctcgaaaag cggcacaaag ttatcatttt
      961 tatttgataa ccgaaatatt tttatcgata aaaaatcaat tatttacaat atacccatgc
     1021 agattcaaac aaagcatcgg atttacggag ctgttccata aattccactg cttcctgata
     1081 acgaggaaat gaacggacaa ccactttgta atttttttcc ggtgaaaata cccgcgcttc
     1141 agtatatttc aaattttgaa gttgggcaca gaatttttca gccgacttgc gggaataaaa
     1201 tgtagctgca ataacgtgat acaagttggt ttgcttgctg agagcgtttt gaaagttttc
     1261 ttcttttgca ggagaaagac tatcttgctt ctccgtctca acagtagctt ttagggtatt
     1321 atttatcgaa acattttttg aagctgaagg ctgggattga gtcggtgtgt atgtttccag
     1381 cggaatcaaa ttggcataat gactgcgctt ggtatcggcc atacgcggag aaaccagcaa
     1441 catgccaaaa attagagcgc aagcagcagc atatcgaaac gaagaagccg atggcaaagt
     1501 aatggtaatt tttttgcgtt cttctgaatc ggtaatacgt cgtttccaca gcttgatttc
     1561 agtcatgcca aaattggcag ggagaaaatc ggcgcgcggc acaggtgaaa aaatcaaatg
     1621 tccgtttgtg tttttttgca ggctgccgag attttccatg tcaacctttt ctccttgctg
     1681 aagtttcgat aaaattgatt ctacttcgtg gtctatcatt tcgatagcgg ctcgatagct
     1741 gatatgttct ctctccgaaa tttcataagc cagcaggcca tcgttggaat ttaacaacgg
     1801 attaaatgca attacggcaa gtggaggcgt tatcctgtcg gataaaatca tggcagattg
     1861 tggttgtata ataaatcctc ccaatcccgg aacgataacg taatcgtgct gagccagcaa
     1921 aatttctatg taccgactta atttttccat gttgcaaata taatagaaag aatttttaaa
     1981 aaggtatttt tacacaaaaa agttttcaac attttgttaa atgtgaaaaa tcgaattatc
     2041 tttgtatgat tcaattcagt tattaacgat ggaaaaaact atactgatta ccggtggtgc
     2101 aggttttatt ggttctcacg tagtgcgcct ttttgtaaat aaatatcccg attaccacat
     2161 tgtaaattta gatgcgttaa cttatgcggg aaatcttgaa aatctcaagg atgtggctga
     2221 tgctcccaat tatacttttg taaaaggaga catcacggat gaagaattta ttcctcaatt
     2281 atttgagaaa tatgattttg acggtgttat tcatttggct gccgaatcgc atgtcgaccg
     2341 atctatcagt gatccgctgg cttttatccg aactaacgta tttggcacag ccaacctttt
     2401 gaatgcagcg aaagaagcat ggaagggcaa aatggaagga aaaagatttt atcatatttc
     2461 taccgatgaa gtttatggtt cgttgggaaa agaaggatat ttcaccgaaa aaacgcctta
     2521 tgatccccga agcccttatt ccgcttccaa agcaagtgcc gaccattttg tcagggctta
     2581 ttaccacacg tatggtttgc cggtattgat ttccaattgt tccaacaatt atggttcgca
     2641 tcatttcccc gaaaaactga ttccgctttg catcaataat attcgctaca atcggccact
     2701 tcccgtttac ggaaagggag aaaatgtgag agactggttg tgggttgatg accacgctgc
     2761 agccatcgac atcatttttc atcaagggga aataggcgaa acttacaaca taggcgggca
     2821 caacgaatgg acaaacattc gtttgataga gaaattgtgc gagattatgg acaaaaaatt
     2881 gggacgtgaa tccggtgaat cagccaaact gataaccttt gtcaaagaca gagcaggaca
     2941 cgatttgcgt tatgccatcg actcgtcaaa attacagcgc gaattaggct ggcagccctc
     3001 cattactttc gaagaagggc tcgaaaaaac agtggattgg tatttggaaa atcaggaatg
     3061 gctcgacaat attattaacg gtgattatca aaactattac gaaaaacaat attccgaaag
     3121 agagaaaagt tgagtatgga ataaattgat tttttgcaat ttcgcctttc acaaaaattt
     3181 cttctactct tattttggtg ctgtctgata aagatagatg ccgatgagca agaatacgat
     3241 attgggcagc catacagcca tgaaaggtga aagcgtccca ttgactgaaa aagtggttga
     3301 cagcgtggaa aacaaaatat atatcgaact gagagccaat ccgattccca aattgagtcc
     3361 catacctccg cgtacttttc gcgacgaaag cgaaactcct attaaagtca tgataaaagc
     3421 agcaaacggc atggcataac ggcgatagta ttcattttcg aaagattgaa tattgcccac
     3481 accacgcgca cgctggcggt gcagatattg tctgagttca gaattggtca tttgagcagc
     3541 ttcttccgaa gtaataaata attctttggg ttgaactttg ataacggtat ccattgaaat
     3601 tcctttcgta attttttcac gcatgccgat aaaatcgcgt tgcaaataat tgttgatttt
     3661 ccagttgtat gccgaatccc atgtaattgt ttctgcggtg aggcgcgaaa ccagtttttt
     3721 cccatcaaat ttttccagtg aaaaatggta cccaatgttg ttgtcgactt cgtatctttc
     3781 gatgtacatg attacacctt tttcaacttc catttgcacg tttcgggcat tgctgggctt
     3841 aaattttttg acatactgat tctcaaaatt caacattttt ttgctggaat ttggaatgac
     3901 atatccaccc agaaagaaaa acaaaagagc aatgagcgtt gcagaaataa aatagggaag
     3961 cattaatctt ctaaaactca caccactgga aagaatggca ataatttcag tattgttggc
     4021 catttttgag gtgaaaaaaa ttacggctat gaacgtgaac agggggctga acatattcat
     4081 gtaatatgga ataaaattca aataataatc aaaaatgatg gctttgaggg gcgcattggt
     4141 ttcaaaaaaa ttatccaact tttcagtcag gtcgaaaaca acagaaatgc tcaaaatcag
     4201 tacgatacag aaaaagtaag ttcccaggaa cttgttgatg atgtacgtat cgattctttt
     4261 aagtccgaaa ctttgataat ttattttcaa tttggttttt aaagctttca tgccagaaaa
     4321 ttttgaataa aggtattttc aaagccttct accgagttgt tcaatcattc cctttttcca
     4381 tagtgaaaaa tctccctttc ggatatgcag tcgggcttcg ttcataagcc aaagataaaa
     4441 agccagatta tgaatagaag caatttgcag tcccaataat tcgttggcag caaacaaatg
     4501 tcgcacataa gctttggaat aagctttatc gacgaatgaa gttccctttt catccagcgg
     4561 cgaaaagtca tttttccatt tggcgttttt tatgttgata acgccttgcg aggtaaaaag
     4621 cattccattt cgaccattgc gcgttggcat gacgcaatcg aacatatcca ctccgcgttc
     4681 aatgctttcc agtatgtttt gtggcgttcc cacgcccatc aggtatcgag gtttgttttc
     4741 gggaagaata tcgtttacaa tctcaatcat ttcatacatt ttttcggtag gttcgcccac
     4801 tgccagtccg ccgatggcgt ttccgtcttt attctggtcg gcaataaatt ttgccgattc
     4861 tcttctcaaa tcgggataga cacatccttg cacaataggg aaaaatgctt gggaatatcc
     4921 gtatttgggt gaaaccgtat cgaaatgttc aattcctctg agcaaccatc gatgagtaag
     4981 ttgcatcgat ttttttgcat attgataatc ggctgtgccc ggtgtacact catccagagc
     5041 cataatgata tctgctccaa tggttcgctg aatgtccact gtaatttcgg gagtgaaaaa
     5101 atgtttgctc ccatcgatgt gcgactgaaa caaaacgcct tcttctttca attttcggat
     5161 accggtaagc gaaaaaactt gaaagcctcc actgtcggtc aatatggggt gatcccagcc
     5221 aataaacttg tgcagtccac ctgcttgttc cagaatgtca gtgccgggtc gcaaatataa
     5281 atgataagta tttcctaaaa tgatttgcgc atgaatgtct tcttccagct cgcgaaaatg
     5341 aacagttttt acggaacctg cggttcctac cggcataaaa ataggtgttt ctattgcgcc
     5401 gtgatcagtg gttattttcc ctgctcttgc cttgctcccg gaatcggtat aaagtatttc
     5461 aaaattcatt cggtgtttct atcaaaaaat tccgttcagc aaatgtttaa aaaattttcc
     5521 agcatttttt gtccaatttc ccccgatttt tccgggtgga actgaacggc aaaaaaattg
     5581 ttcttttgta aggctgcact gaagtcgaaa atataattgg caacagcagc ggtatttgac
     5641 gacagtggta cgtagtaact gtggacaaaa taaacaaacg aatttttttt tatgccatca
     5701 aacaatggtc cggataaccg aaaaatatta ttccagccca tttgtggaac tttaaaattt
     5761 tcgttggcag gaaaatgttt tactttttca tcaaagatac ccagacaagt tgtattgttt
     5821 tcttcagaga aggagcacat gagttgcatg cccagacaga ttcccaatac cggttgtttg
     5881 agtgaaggaa taagcaggtc gagccggtta tgcttcagat agttcatggc tgtagaagct
     5941 tcacctacac cgggaaaaat taccttgtca gcagacagga tttcttctgc gtcatcagtt
     6001 acaaccgctt caattcccag tcgttccaaa gcaaaaagta ccgatcggat gttccctgca
     6061 ttgtatttca ccaaagcaac tttcattgtc tcaaccggtc tttatttttt caattggttg
     6121 gtttctgtat cgggataaat aacaaccggt gtaaactttt tggcttcttc aaagtccatt
     6181 gtagcgaaag ccatgatgat gaccacgtcg ccaatttgaa ctttgcgcgc agctgcacca
     6241 ttcaaacaaa ccatacctga gcctctttct ccgggaataa cgtaagtctc aattctttca
     6301 ccattattgt tgttgaccac acttactttt tcgtatggaa taatgttggc tgcatccatc
     6361 aaatcctggt cgagcgtaat gctgccgata tagtttaaat tggcatctgt taccgaaacc
     6421 ctgtgaattt tcgattttag aatttctatt tgcatatttt caaattcttt gtgttaaaga
     6481 aataaagctg cagttgtgca acttattgaa ggtcattgta tttaatatta tcaatcagtc
     6541 tcacttcacc gcaaaaaaca gctatgcagc caatggcagg ctctgaccac gaatgaatgg
     6601 gttgcagtgt agtttgatga acgatttcaa agtattctac ttccaagccc gaaaaggaat
     6661 tcaatcgggc aatcacccaa ttttgtactt gttcagggga aaagtttgaa atcagttttt
     6721 tactttcaaa taaagttttg gaaatttcaa cggctttttg acgttcttcc gcattgagcc
     6781 ttaaattgcg gctgctcatg gccaaaccat ctttttcacg cacaatggga caaccaacaa
     6841 tttcgatatc gaaattcatt aagtttacca ttcgacgaat gatggccaac tgttgaaaat
     6901 ctttttcacc aaaataggct ttggtaggtt gcaccaagtt aaacaatttg ctgacaacct
     6961 gcacaacacc attgaaatgt cccggcctga attttccttc cataacctgg tccaatccgc
     7021 caaaatcaaa ctcaaaaaca ctttcaaatt cgtttttgtc gtacatttcg tcaacagagg
     7081 gagagaaaac aatatcgcaa ccgttttttt cgagcaacag caaatcccgt tgcaaatcgc
     7141 gagggtattt ttccaaatcc gaccggttgt taaattgagt aggattgaca aaaatactta
     7201 ctgcggtaac atcattttcg gctacgctac gattgatcag ggacaaatga ccctcgtgca
     7261 aagcgcccat ggttggaaca aatccgactg tttttccttc tttcctgatt tgctccatct
     7321 ccttttgaag ctcctttttg gttgagaata ctttcatgcg ttttcaccat tttttcaaca
     7381 agtcgaacag aacttttgac gctaacttta aagcagtcga atcaaagatt ttcagttccg
     7441 aaagacctaa ttttttgaaa atttgacagt tccaattttg aataccatca aaattgggtt
     7501 acaaaattac gaaaacttat ttggatatgc acgattgctt cttaattttt cctaaagcaa
     7561 gccgaagacc aattgtataa actttggaac gttgaatcta ctatctacat ttttaaaaat
     7621 tttctttgct cgttttaaaa atatacatta tctttgcact tcgaaaatgg ccccgtggct
     7681 caactgaata gagcatctga ctacggatca gaaggttaca ggtttgaatc ctgtcggggt
     7741 cacatttaat ttaaaaaag
//
LOCUS       DAOTAX010000072         7739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8051_length_7739_cov_28.782145, whole genome shotgun sequence.
ACCESSION   DAOTAX010000072 DAOTAX010000000
VERSION     DAOTAX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_7739_cov_28.782145"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..2777)
                     /locus_tag="PLM04_07270"
     CDS             complement(222..2777)
                     /locus_tag="PLM04_07270"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HON02909.1"
                     /translation="MNSFIIKQLKNYAILLLFIQSFQALSAQKTEVPFSTDSNYLTVW
                     NGKKYIPIFIKGVNLGVAKPGSYPGELDVKPEQYRQWFEEIKDAGFNCIRLYTLHFPQ
                     FYDELLKYNTNHPQHPLFFFQGVWLNEEMEGYKNDLYQITDTFRAEIRENIDCVHGNK
                     LIAQRYGKAYGNYTADVSQWNIGYIVGREVYPEEILTTDSIHADNNHFTGTHFEINNA
                     SPSEVWFTENLNHLVEYEKNTYNTERPVSISSWPTLDPLHHAFEPNRTEDTATVDLSK
                     INILDAPAGLFISYHAYPYYPDFVGAQPDYKLASDNFGSNSYLGYIRDLKNHYRKYPL
                     LIAEYGVPSSWGIAHYTSSGMNHGGFDEQQQGETDLRLLRNISSSQLAGGIQFAWIDE
                     WFKKTWITDPIDFGNRILWHNITAAEQNFGLKKFVKSDEWKIWKNFDAADDITTLSAK
                     QNYDFFEIQLNLKDKMDLLGECWIAIDTYDAGLGESMMPNGQTIPSRAEFLLKITQYA
                     ATLYVTEAYDLFGLWHKILTPKQKLRSVATDGATWNIVRWKNNSFYSDVQYIGELKLN
                     KSFQPETSKDAVTIYDDKISIRLPWTLLQFVDPSQKEVFHVDSLNPELRTRESDGIAV
                     SIFYKNKTYFSDTRFDWEKWNKVTDNDVEEKFKSSYWTMYENLTEFNTPAIAFPDNYE
                     LEGEESIFKIDEYKGVLKNDFDMDGWNISAVLTDAPANGFVEINVDGSFSYIPRKGFK
                     GTDVFKYAIFDGTSLSQNTTVTLHVKKEGKKNPDNKKFFSLFPNPASDIVSIKSEINI
                     VSVRIFNYSGKLMEEFLLNDKTGTLNISHYPAGLYIAMIDVAGQLFTEKFIKK"
     gene            complement(2789..3229)
                     /locus_tag="PLM04_07275"
     CDS             complement(2789..3229)
                     /locus_tag="PLM04_07275"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HON02910.1"
                     /translation="MKSAESSDKKNSDPVIPFKFDIEPIDSQHQKLFRIFQRILDNIE
                     QKSGNTEQIIEELEEYTHYHFSTEEDLMKKFDYQEINEHIVQHEIFIQKMLDFKTSQL
                     FQSITLNEEIAGFLRKWFLNHILEYDAKYVDTFKKNLNSENEEK"
     gene            complement(3322..4773)
                     /locus_tag="PLM04_07280"
     CDS             complement(3322..4773)
                     /locus_tag="PLM04_07280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02911.1"
                     /translation="MEFPIIIEIVNWLIFVYACALFISYLLIALLSTSELKKYHSRNR
                     YSDFLKMLSFQKLPSVSIIAPAYNEEKTIVDNINCLLSLKYKDFEIIIVNDGSKDQTL
                     NKIIQYFDLIKTDMAYESQIITKNIRGIYKSRNNAYNHLTIIDKENGGKADALNAGLN
                     YAHNDLFLAVDVDSILEPDALLRMVKPFIEETKVKVIASGGVIRVANSCEIENGRIKK
                     VNFPKNQWAKYQTIEYFRAFTLGRMAWSRINGLLIISGAFGMFDRQTALAAGGYDTST
                     VGEDLELVVRMRRYMHEVEKKKYKIAFIPDPLCWTEVPESLKMLSRQRNRWTRGAIET
                     IRKHMKMAFNPKYGRIGMISFPYWIFFEWLAPIIQFLGIGYVILLTIFGLLDVKVFLI
                     MTLFVLSFAVMYSFFSVLLEAIYYNKYKNVTYLGNILLLSVMEMFVYQPINMLFSLSG
                     NIDYFFKKKSRHIWGNMTRKGFSGNTDQNSKQN"
     gene            complement(4779..5990)
                     /locus_tag="PLM04_07285"
     CDS             complement(4779..5990)
                     /locus_tag="PLM04_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02912.1"
                     /translation="MRTISLYLKFLFSRLIFIIALCLAVFLFFFLKQKEFILFNYSIG
                     NPLYSIIEQSPSWLYVVQVLIVIFLLLIVAELISIVLVTTRRSSAEKKNQLLHERIRE
                     VIFNYLIQDNKTRDINDFRIKAQPYLKSNHAQRVFINQLRKILILTKGEVHETCISLY
                     DALHFDPLIKSHFHSPYLRNKLFALKTAGDFQLTKYNRYLVRYMHSKNEMLRSEALQA
                     YVKANSDNDLSFLSDYQEKISLWDFNIIINAAKDRTNINFSALLKSHNPLIKSLGLRL
                     ANLQKKEELKPIIFKLMNDADPMVKEEAYLAFMALAKEKNDYLTLMYQFSCMTKNLQN
                     KVINLLINNEDKTSSYNFFEWIIENKPLEMKIRAMSKLLTMDMNLVLKYKNHPDENIR
                     KALRQLMDFYL"
     gene            complement(5994..7247)
                     /locus_tag="PLM04_07290"
     CDS             complement(5994..7247)
                     /locus_tag="PLM04_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HON02913.1"
                     /translation="MKKKFQLIVFLIMLASTGFLQAQTNVDSIFAVAIEKAHGGAYAA
                     AIREAKTALSADTARGDICVFLANVYSWSQKNDSALLFIDKAKKLNYLNEEFYDSYLN
                     ILLRSGKYEELEAACEEAEKNNYGDTKNLLTKKLIAYAQTGAYEKGIGLFKDEKNKVY
                     LQEKTIDELYTYLLMKQRTNLLSVNYSLDMFDNNDPQHLFSLGNAMQFKNRNIGLNAN
                     YAHRFGKNDVQIELNDYSVIYKKNYLYFNYGYALTGDLFPRHRTGLEYYFAISKHWDG
                     SLGGRYMFYPNAENKNVVIFTGNVGHYFGNNWASLRPFYVVQNNLQSLSVVFKYRLYG
                     SDNRNFWGLEAGVGNSPDDILTTSQSSFNELMSYRIRLEKNFKVNRISDVNIGLGYMY
                     EQINQNATKQFRNRYLVEVGYKYRF"
     gene            complement(7272..7661)
                     /locus_tag="PLM04_07295"
     CDS             complement(7272..7661)
                     /locus_tag="PLM04_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HON02914.1"
                     /translation="MAYRINIISNEIVFLTLIRNFIKKIELDIQISETDSLNMLYKEL
                     KENSFDLIIVDGNFSENSAIDLLYIIRFKLKIFTPIWFFTVIQTPEYYNKALQVGANV
                     IIERPFDPIEIASQIHEFLHNKVSVKN"
BASE COUNT         2288 a         1368 c         1445 g         2638 t
ORIGIN      
        1 gagcatcaaa ttccctggca gacaaacaaa ctcaccggag gtttatattt ttacaaaatt
       61 cagacagaag attttagtgt tacgagcaaa tttgtggttc taaaataaaa gttttttcag
      121 ttattggcgt agaattgatt ttttgaattt tttcagctca ttataaaaca aaatatggaa
      181 gaaagtttgt tgaaaagaac aggctttctt cctttatttt ttcatttttt gatgaatttt
      241 tcggtaaaca gttgtccggc cacatcaatc atggcgatgt ataggccggc tggataatgg
      301 gaaatgttta aagttcctgt tttgtcgttc agcaaaaatt cttccattaa ttttccggaa
      361 taattaaaaa ttcgtacgga aacaatattg atttccgatt ttatggaaac aatatcggaa
      421 gcaggattgg ggaagagcga aaaaaacttt ttgttgtcgg gatttttttt accttctttt
      481 ttgacatgta aggttacggt tgtgttttgg gatagagaag taccgtcaaa aattgcgtat
      541 ttgaacacat ccgtaccttt aaatccttta cgaggaatgt aagaaaacga accatcaacg
      601 tttatttcga cgaaaccgtt ggcaggtgca tcggttaaaa cagcagaaat gttccaacca
      661 tccatgtcaa aatcattttt tagtacacct ttgtattcat cgattttaaa aatactttct
      721 tctccctcca gctcataatt gtcgggaaaa gcaatggccg gagtattgaa ttcggtgaga
      781 ttttcgtaca tagtccagta ggaagatttg aatttttcct ccacatcgtt atcggtaact
      841 ttgttccatt tttcccagtc gaagcgcgta tcgctgaaat aggttttatt tttgtaaaaa
      901 atgctcaccg caataccatc cgattctctt gtacgtagct caggatttaa actgtcaacg
      961 tggaaaactt ctttttgaga cggatcgaca aattgcaata aagtccaggg caatcggatt
     1021 gaaattttgt cgtcataaat tgtaacggca tcttttgaag tttccggctg aaaggattta
     1081 ttcagtttta attctccaat gtattgaaca tccgaataaa aagagttgtt tttccagcgg
     1141 acaatgttcc atgtagcgcc atctgttgcc acagaacgca acttttgttt tggtgtgagt
     1201 attttgtgcc acaatccgaa taaatcataa gcttccgtaa catacaaggt tgctgcatat
     1261 tgagtgattt tgagcaaaaa ttcggcacgt gaggggatgg tttgtccgtt tggcatcatg
     1321 gattctccca aacctgcatc gtacgtatcg attgctatcc aacattctcc caacaaatcc
     1381 attttatctt tcagattcaa ctgaatttca aaaaaatcgt aattttgttt tgctgacaaa
     1441 gtcgttatgt catcagcagc atcaaaattt ttccagattt tccattcgtc ggattttaca
     1501 aattttttca ggccaaagtt ttgttcggca gcggttatgt tgtgccataa aattctgttg
     1561 ccaaaatcaa taggatcggt aatccatgtt tttttgaacc attcgtcaat ccacgcaaat
     1621 tgtattcctc cggccagttg actggatgaa atgtttctga gaagacgcaa atcggtttcg
     1681 ccttgctgct gttcatcaaa tccaccgtga ttcatacctg aagaagtata gtgagctatg
     1741 ccccaactcg aaggaacgcc atattcggca ataagcaaag gatattttcg atagtggttt
     1801 tttaaatctc tgatatagcc caaataactg ttgctgccaa aattatccga tgcaagttta
     1861 taatcgggct gagcccctac aaaatccgga taatagggat aggcatgata actaataaaa
     1921 agtccggccg gagcatccag tatattgatt ttcgataagt ctacagttgc cgtgtcttcc
     1981 gttctatttg gttcaaaagc atgatgcagg ggatcgaggg tgggccagga agaaatgctg
     2041 acaggtcttt cggtgttgta tgtatttttt tcgtattcta ccaaatgatt caaattttct
     2101 gtaaaccaaa cttcggatgg tgaagcgtta ttaatttcaa aatgggtacc tgtgaagtga
     2161 ttattgtccg cgtgaatact gtcggtagtc agaatttctt caggatatac ctctcgaccg
     2221 acaatataac cgatgttcca ttgcgaaacg tcggcagtat agttcccata agcttttccg
     2281 taacgctgcg caatcagttt gtttccatga acgcagtcaa tgttttccct gatttcggct
     2341 ctgaaggtgt cggtaatttg atacaaatcg tttttgtatc cttccatttc ttcgtttagc
     2401 catacgcctt gaaaaaagaa taaaggatgc tgcggatggt tggtattgta tttgagcaat
     2461 tcgtcataaa attgtggaaa atgaagtgtg taaaggcgaa tacaattgaa tccggcatct
     2521 tttatttctt caaaccattg gcgatattgt tcgggcttca catccagctc gcccggatac
     2581 gaaccgggtt tggcaacacc caaattgact cctttgataa aaatgggaat atattttttt
     2641 ccgttccaaa ccgtcagata gttgctgtct gtggaaaagg gaacttctgt tttttgcgct
     2701 gaaagagctt gaaaagattg aatgaacaaa agtaggatag cgtaattttt cagttgtttt
     2761 attatgaaac tattcatgta atattttttc atttttcttc attttcggag tttaaatttt
     2821 ttttaaaggt atccacgtat ttggcatcat attccaatat gtggttgaga aaccacttgc
     2881 gtagaaagcc ggcaatttct tcatttaaag taatactttg aaataattga gaagttttaa
     2941 aatcgagcat tttttgtata aaaatttcat gttgaacaat gtgctcattt atttcctggt
     3001 agtcgaattt tttcatcaga tcttcttcgg tactgaaatg ataatgggta tattcttcca
     3061 gttcttcgat aatttgttcc gtatttccag atttttgttc aatgttatcc aagattcttt
     3121 gaaatatacg aaacagtttt tggtgttggc tgtcaattgg ttcaatatca aacttaaatg
     3181 gaattacagg gtcggaattt tttttatccg aactttctgc gcttttcata acgattgttt
     3241 ttttaatgtg cttttatttt ctttttcata taaattcaag aaatctaaaa aataatcaga
     3301 tttttaagta aaatactttc cttaattctg ctttgaattt tgatcggtat ttcccgaaaa
     3361 accttttctg gtcatgtttc cccagatatg tctgcttttt ttcttaaaga aataatcgat
     3421 gtttcccgac aaggaaaata gcatattaat cggctgataa acaaacattt ccattacaga
     3481 caagagtaaa atattgccca aataggtaac atttttatat ttgttgtaat aaattgcttc
     3541 taaaagtacg gaaaaaaatg aatacatgac agcaaaagaa agaacaaaaa gtgtcataat
     3601 caggaatact tttacatcca acagcccgaa aatagtcagc aaaattacgt agccaatgcc
     3661 caaaaactga ataattggtg ccaaccattc gaaaaatatc caataaggga agctgatcat
     3721 tccgattcgt ccatattttg gattaaaagc cattttcatg tgttttcgta tggtttcgat
     3781 ggctccgcgt gtccatcggt ttctttgtcg cgaaagcatt tttaatgatt cgggaacttc
     3841 cgtccagcac aatggatcag ggataaaggc tattttgtat ttcttttttt ctacttcgtg
     3901 catgtaacgg cgcattctga caaccagttc caaatcctcg cctacagtgg aagtatcata
     3961 gcctcctgct gccaatgctg tttggcggtc gaacatgccg aaagcgccag aaattatgag
     4021 caatccgttg attctgctcc aagccatacg ccctaaggta aatgcccgga aatattcgat
     4081 ggtctgatat tttgcccatt ggtttttcgg gaaattcacc ttttttattc tgccgttttc
     4141 tatttcgcag gagttggcta cacgaattac tccgcccgaa gcaattacct tgactttggt
     4201 ttcttcaata aaaggtttta ccattcgcag caatgcatcg ggttcgagaa tggaatcaac
     4261 atcaacggcc agaaacaaat cattgtgcgc ataattcaag ccggcattca gtgcatcggc
     4321 ttttcctcca ttttccttgt cgatgatggt caaatgattg taagcgttgt ttcgtgattt
     4381 gtaaattccc ctgatatttt ttgttataat ttgtgattcg taagccatgt cagttttaat
     4441 caggtcgaaa tattgaataa ttttattgag tgtttggtct ttactgccgt cgtttacaat
     4501 gataatttca aaatccttgt atttcaagct taaaaggcag tttatattat ccacaattgt
     4561 tttttcttcg ttgtaagccg gagctataat tgaaactgaa ggcaatttct gaaaactcaa
     4621 cattttcaaa aaatctgaat atctgtttct tgaatgatat ttttttagtt ctgatgttga
     4681 caaaagcgct atcagaaggt aggagataaa aagggcacat gcataaacaa agatgagcca
     4741 gttcacaatt tcgataatga tgggaaattc cataattttt aaagataaaa gtccattaat
     4801 tgacggagtg cttttctgat attttcgtca gggtggtttt tgtatttcaa aaccaaattc
     4861 atgtccattg ttagcagttt tgacattgcc ctgattttca tttccagcgg tttgttttcg
     4921 atgatccatt caaaaaaatt gtacgaactt gttttatctt cattattgat cagaagatta
     4981 ataactttat tttgtaaatt ttttgtcatg caactgaact gatacattaa agtcaggtaa
     5041 tcgttttttt ctttggctaa tgccataaat gccagataag cttcctcttt caccattggg
     5101 tcggcatcat tcatcagttt aaaaataatc ggtttaagtt cctctttttt ttgtaggttg
     5161 gcaagtctta atcccaaact ctttattaat gggttgtgag atttcaaaag agcggaaaaa
     5221 tttatatttg ttctgtcttt tgctgcattt atgataatgt taaagtccca gagagaaatt
     5281 ttttcctgat aatccgacag aaaactgagg tcgttatcgg aattggcttt tacataagcc
     5341 tgcagtgctt ccgatcgtaa catctcgttt ttactgtgca tatagcggac aagatatcgg
     5401 ttgtattttg tcagttgaaa atcacctgcc gttttcagag caaataattt gtttctgagg
     5461 tatggagaat gaaaatgact ttttataagc ggatcaaaat gcaacgcatc atacaaagaa
     5521 atacaggttt cgtgaacttc gcctttggtg aggataagaa ttttacgcag ttgattgata
     5581 aaaactcttt gggcatgatt ggatttcaag tagggttgag cttttatacg aaaatcattg
     5641 atgtctcttg ttttgttatc ctgaatcaga tagttgaaaa tcacttcacg aatcctttca
     5701 tggaggagtt ggttcttttt ttcggcagaa gatcttcgag ttgtaaccaa aacaatcgaa
     5761 atcagttcag caacaatcag caacaggaag ataacaatca gcacctgaac cacataaagc
     5821 catgaaggcg actgttcgat aatggaatac aaaggattgc caatggaata gttaaacagg
     5881 ataaattctt tctgtttcag aaagaaaaac aaaaatacgg ccagacacaa ggctatgata
     5941 aaaatcaacc ttgaaaaaag gaatttcaga tataagctga ttgttctcat ttttcagaat
     6001 cggtatttgt agccaacttc cacaagatag cggttgcgga actgttttgt ggcattttga
     6061 ttgatttgtt cgtacatata acccaacccg atattaacat ctgaaattct gttcacctta
     6121 aagttctttt ccaacctgat gcgataggac attaattcgt taaatgaact ttgtgaagtg
     6181 gtcagtatat cgtcgggaga attgcctacg ccggcttcca agccccaaaa attcctgttg
     6241 tcgcttccgt acagacgata tttgaatacg accgaaagcg attgcaaatt gttttgtacc
     6301 acataaaaag gccttaatga agcccaatta tttccgaaat aatgtcccac attgccggta
     6361 aagataacaa catttttgtt ttctgcgttt ggataaaaca tgtatcgacc gcccagtgag
     6421 ccatcccaat gcttgcttat ggcaaaataa tattccagtc ccgttcggtg acggggaaac
     6481 aaatcgccag taagagcata tccataattg aaataaagat agtttttttt gtaaataacg
     6541 gaataatcat tcaattcgat ttgcacatca ttttttccga aacgatgtgc atagttggca
     6601 ttaagtccga tatttctgtt tttaaactgc atggcattgc ccaaagaaaa aagatgttgc
     6661 ggatcgttgt tgtcgaacat gtcgagcgaa taatttaccg aaagcaaatt ggtgcgctgt
     6721 ttcatcagca gataagtgta aagttcatca atggtttttt cttgcagata aactttgttt
     6781 ttttcgtctt taaacaaacc gattcctttt tcgtatgcac cggtttgagc ataggcaatg
     6841 agtttttttg tcaacaggtt tttagtatcg ccataattgt tcttttctgc ttcttcgcag
     6901 gcagcttcta attcttcata tttccctgaa cgcagcaaaa tattgaggta actgtcataa
     6961 aattcttcat tgagatagtt caattttttt gccttgtcta taaacagtaa agcagaatcg
     7021 tttttttgcg accacgagta aacgttagcc aaaaaaacac aaatatcacc ccgagcagta
     7081 tcagcagaca aagccgtttt agcttcccga atggctgctg cataagctcc accatgagct
     7141 ttttctatag caacggcgaa tatggaatcg acattggttt gagcttgcag aaaaccagtt
     7201 gaggcaagca tgatcagaaa aacaatcagt tgaaattttt ttttcatatt gttggttcgt
     7261 ttgtataact ttcaattttt tacagagact ttattgtgca gaaattcgtg aatttgtgag
     7321 gcaatttcta tgggatcgaa tggcctttca ataataacat tggcaccaac ctgaagtgct
     7381 ttgttgtaat attcgggcgt ttgaataacc gtaaaaaacc agataggagt aaaaattttc
     7441 agcttgaatc gaatgatata caacaagtca atggcagagt tttctgaaaa gttgccatct
     7501 acaataatta aatcgaagct attttctttc agttctttat acagcatgtt gagcgaatcg
     7561 gtttcactga tttgaatatc caattcaatt tttttgataa agtttctgat taatgtcaag
     7621 aaaacgattt cgtttgaaat tatattgatt cggtatgcca tttaataatt ctttttttga
     7681 ttctgaattt taaaaattgt gtttattctg ttcgctgata ttcttcgttt aaaatttgt
//
LOCUS       DAOTAX010000073         7717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8079_length_7717_cov_31.153876, whole genome shotgun sequence.
ACCESSION   DAOTAX010000073 DAOTAX010000000
VERSION     DAOTAX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8079_length_7717_cov_31.153876"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..608
                     /locus_tag="PLM04_07300"
     CDS             78..608
                     /locus_tag="PLM04_07300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HON02915.1"
                     /translation="MINTTAFHSACFGFASTPFGKCCVAKDDRGIFSLTFPFDEEFAI
                     NELFGKFPDKKFYHDDKEIGIMAQLIFQRNICPALNFYGSDFQKTVWEALLKIPFGGR
                     VSYEEIAISIGMPKAVRSVAHAIACNPIAFLVPCHRVILKNGSIGEFRWGRERKVAML
                     EWEEKILNLEKFQEIE"
     gene            792..1076
                     /locus_tag="PLM04_07305"
     CDS             792..1076
                     /locus_tag="PLM04_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02916.1"
                     /translation="MKQVNSIYRFQLQIGAIKTTSLIEGFIIGVWCGDNQFLHIEFTR
                     NDAAQGKILVEKIHRLKTFINASSPSSHRLPTNFEEEEVYKDYCYSYSAL"
     gene            complement(1510..2619)
                     /locus_tag="PLM04_07310"
     CDS             complement(1510..2619)
                     /locus_tag="PLM04_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HON02917.1"
                     /translation="MENKFVTLAIRTYKRAQMIKTTLEENGIETRIESVNLEHPEMSV
                     GVRIRIKENDLPRALKIVEEMEIAWEEKGETLKRAEKMILLPIDFSDQLPKIIDFGFY
                     FAESLNAKVELLYVYYSPPFTISSVNNEVNTYTLSDSELLRQIISNANREVTSMTNTI
                     NERIAKGELPNVPFHFELREGVPEDQIVDFCKKYQPALVIMGTRGKKISSELIGSVTA
                     EVIESCVSPVLALPVDMKINTPSEIKRIAFLTNFEQKDLIAIDNTISLFNWNSLEMFF
                     IHAAQKKEVWDEVMLAGIKEYFSKHYPKIITHYELIDSTETMKQIDDYLVKHDINLLA
                     FNSPRRNLIRRLFNPGLAFKMVLHSDTPLFVTHIS"
     gene            2981..4288
                     /gene="gldE"
                     /locus_tag="PLM04_07315"
     CDS             2981..4288
                     /gene="gldE"
                     /locus_tag="PLM04_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HON02918.1"
                     /translation="MIAFHSFSVSAAIALLLSILLLMCSAIISGSEVAFFSLTPQMKN
                     EVEESNRKNAKNLMHLIGNPQQLLATILVGNNFVNVAIIMLLTYFTGSVFDFSTFPLL
                     GFIFETVIITFVLLLFGEIIPKVYASQYPEKMALLFTPFILVMQKVFSRVSKLLISST
                     AFLTRQIEKYKKAEISIDELSHALELTTDEQDDDKEMLEGIIKFGNIRVAEIMTSRVD
                     MIAVDTKTNFKDLLKIIIQTGYSRIPVYSENRDNIKGILYSKDLLPHLDKPANFRWQS
                     LIRQAYYVPETKKIDDLLSEFQKNKVHLALVVDEYGGISGLVTLEDILEEIVGDISDE
                     YDDEEKLYTKVDNHTYIFEAKILLNDFFKVTGINEEEFSKVTEEVETLAGLVLELKGE
                     MPAKNEMIEYGRYVFEILSVDNRRIRKIKLHMKDNFDRNKEEN"
     gene            4349..4954
                     /gene="gldD"
                     /locus_tag="PLM04_07320"
     CDS             4349..4954
                     /gene="gldD"
                     /locus_tag="PLM04_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HON02919.1"
                     /translation="METKKIKYLQTIFYKTTLLIVITASLFSCGKNYVPRPYGYFRVD
                     LPPHNYRYLDTLQLPYRFEISSYAKVEKREAEGEKCWIDIKYPFLNAAIYCSYKPIHG
                     NLKELLEDTRQMAYKHSIRADAINEKLYQHPEKKVYGILYDLKGNTASSVQFFVTDSI
                     HHFFRGSLYFDNVPNKDSIAPMSDYIRQDIIRLMESFEWKQ"
     gene            4951..5595
                     /locus_tag="PLM04_07325"
     CDS             4951..5595
                     /locus_tag="PLM04_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HON02920.1"
                     /translation="MIHRKIETSGAKILVWQSTETFDELVTFFDKNETEELNTLHSEK
                     RKIEYLSVRLALKTLLNREIKICYNDDGKPFLSDYFAEIGISHSGQWIAVITHPTCKV
                     GIDIERFQPKISAISKRFLSKEELEKIFEKDENRLKKLTLLWSAKESLFKVIGKEAVD
                     FSRQLCILPFEMGKTGKIKALHIPRNKLYELNYELSEEYAMVYLIDKNVEPNKL"
     gene            5647..6399
                     /locus_tag="PLM04_07330"
     CDS             5647..6399
                     /locus_tag="PLM04_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HON02921.1"
                     /translation="MKNLYKQILINREYNKKMLAVLLDPDRCQGKLMSSTIAALKLSA
                     PDFVFVGGSYVNSPIDSFIELLHEEVQIPVVLFPGNASQFSPKADALLFLSLISGRNP
                     EFLIGQHVTSALAIKQSGIEVIPAGYILIDGGKVSAVEYMSHTRSIPREKKEIVLATA
                     VAGELSGQHVVYLEAGSGAIQPVPKELINHVTSHLSIPVIVGGGMKTTQHLNDCFDAG
                     ADLAVVGNAVEQNPDLIVEFTNFVKNYNTIHR"
     gene            complement(7354..>7717)
                     /locus_tag="PLM04_07335"
     CDS             complement(7354..>7717)
                     /locus_tag="PLM04_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HON02922.1"
                     /translation="KLEKLPGSDQAKWLNISPKEMEEKIYKIQGPQPEVQVTQESRQR
                     GFDNYQTTDDHGMLIFGIAKDQNGTKYYLVKNSWGTDNKYKGIWYASESFVKDKTINI
                     LVHKNAIPKEIKTKLGIK"
BASE COUNT         2420 a         1359 c         1438 g         2500 t
ORIGIN      
        1 actacaaaaa tatgaaattc tcaggcattt ttaatttcta aagtttcgga tagtaatgaa
       61 cttttcacat ttgcttgatg ataaatacaa cagctttcca tagtgcttgt tttggttttg
      121 cttcgacccc ttttgggaaa tgttgcgtgg ctaaagatga tcggggaatt ttttcgctta
      181 cttttccttt tgatgaagaa tttgcaataa atgaattgtt tgggaaattt ccagataaaa
      241 aattttatca tgatgataag gaaataggaa taatggctca gctcattttc caaagaaata
      301 tttgtccagc attaaatttt tatggatcag attttcagaa aaccgtctgg gaagccttgc
      361 tgaagattcc ttttggaggt agagtttctt atgaagaaat tgccataagt attggcatgc
      421 ctaaagcagt tcgctcagta gctcatgcta tagcgtgcaa tccgatagca tttctggttc
      481 cctgtcatag agtgattctt aaaaacggaa gtattggaga atttcgttgg ggaagggaaa
      541 gaaaagttgc tatgttggag tgggaagaga aaattttaaa tctcgaaaaa tttcaggaaa
      601 tcgagtaaat gttctcggta aaaaactctt ttttagattt tttatcgcaa cacgatcacg
      661 taaccgttct gtcaaagact ttgcaggtgt ccattttttt aagcacagaa ttcacagtag
      721 gaaaataaag ttttaattta attttgtaaa aaactcaaat gcccggcaat ataaaatgac
      781 gtttatgaac tttgaagcaa gtaaactcaa tttatcggtt tcagcttcaa atcggagcta
      841 taaaaacaac ttctcttatt gaaggattca taatcggtgt atggtgtgga gacaatcaat
      901 ttttacatat agaatttacc cgaaacgatg cagcacaggg gaagattttg gttgaaaaaa
      961 tacaccggct caagacattt ataaacgctt cttccccaag ttcacacagg ttaccaacca
     1021 attttgagga ggaagaagtt tacaaggatt attgttattc ttattctgct ttgtaaccac
     1081 catgaaactt tcagcaccag agagatatgg agaatatacc gaagaagaga aaagtgctga
     1141 agacaaaata aagaaattaa aatatgactt tgtctttgac agttttaacc taaaagtctt
     1201 ttttatgtaa ctgaagcagc tttaattttt gtgaggatag cccatcatct tatgtcgctg
     1261 tttagaatgc ttatttggca gggaaaggcg caaaaaacta tctccatact gctgatttga
     1321 acttttgccg taagtgcata ttttgaaaaa tcaacagcag accgtttctc aaaatcgctt
     1381 taaaataaaa aagcgagtct ggtttttcca gagtgtggaa cagttgttct gaacttgact
     1441 ttccctttaa attggctaat gaataatctg ggttaaaatc taacaacaaa cattgcaaat
     1501 tgaaaatgtt tatgaaatgt gcgttacaaa caaaggtgta tccgaatgta aaaccatttt
     1561 gaaagctaaa ccgggattaa atagccgtcg gataaggttg cggcgtggag aattgaatgc
     1621 cagcaaatta atatcgtgtt tgaccaaata atcgtcaatt tgtttcatgg tttcggtaga
     1681 atctattaat tcataatgag taataatttt agggtaatgt ttggagaagt attcttttat
     1741 tcctgcgagc atcacttcgt cccatacttc ctttttttgt gcagcgtgaa taaagaacat
     1801 ttccagggaa ttccaattaa aaagtgaaat ggtattgtca atggctataa ggtctttttg
     1861 ttcgaaattt gtcaagaaag ctatccgttt gatttcggaa ggtgtattaa ttttcatgtc
     1921 aacagggaga gccaataccg gagatacgca agattctata acttctgctg ttacacttcc
     1981 gataagctca ctgctgatct tcttccctcg tgtccccatg atcaccaaag caggttgata
     2041 ttttttacaa aagtcaacaa tttgatcttc cggtacgcct tccctgagtt caaagtggaa
     2101 agggacatta ggcaattctc ctttagcaat tcgttcgtta atggtattgg tcatggaagt
     2161 tacttcacga ttagcatttg aaatgatttg acggagtaat tcgctatcgc tcagagtata
     2221 agtgttgact tcgttgttta ccgaagaaat tgtaaaagga gggctatagt aaacataaag
     2281 cagttcgacc ttggcattta aagattctgc aaaataaaaa ccaaaatcga taattttagg
     2341 caactgatca gaaaaatcta taggcagcag tatcattttt tctgctcttt taagagtttc
     2401 ccctttttct tcccacgcaa tttccatttc ctctacaatt ttcagagcac ggggaaggtc
     2461 attttcttta attctgatac gaacacctac cgacatttcg ggatgttcca gattcacact
     2521 ttctatccgg gtttctatcc cgttttcttc gagcgttgtt ttgatcattt gagccctttt
     2581 gtaagtgcga atagcgagcg ttacaaattt attttccatg atttctaaga ttttagtttt
     2641 taataggaaa ctttcctttg tcctatcctt tcattttcgt gtaaagttaa taatttttat
     2701 gatatgtatc atccagtttt tgtttttttt ctaaaaacag aaattaacct tatatttgca
     2761 tttaaaataa acttttttaa aacagcttta aagcgtttcc ctttacataa aacgcttcta
     2821 taagcaaatt tgttttaaga atttttttgt ttgttaaggc ttagttgcaa gaaattatat
     2881 ttgaaataga caagttcttt ggtatgagag taaaaaaatt tctaccacta actgttttag
     2941 aattaagata ataactattc ttttatcaca tattaatgca atgatagcat ttcattcttt
     3001 ttctgtttcg gccgcaattg cacttttgtt gagtatatta ttgctaatgt gttcggcaat
     3061 tatttcaggt tcggaggtag cttttttttc tcttactcca cagatgaaaa atgaagttga
     3121 agagagcaac agaaaaaacg caaaaaatct gatgcactta attggcaatc ctcaacagct
     3181 gttggcaacc attttggttg ggaataattt tgttaatgtg gccattatca tgttgctgac
     3241 gtattttaca ggctccgtgt tcgatttttc tacttttcct ttgctgggat ttattttcga
     3301 aacggtgatc ataacttttg tattgcttct ttttggtgaa atcataccta aggtttacgc
     3361 atcgcaatat cccgaaaaaa tggcattatt gtttactcca tttattttgg ttatgcaaaa
     3421 agtgtttagt cgggtttcca aattgctgat ttcttctact gcttttctta cccgtcagat
     3481 agaaaaatat aaaaaagccg aaatatcgat cgatgaactc tctcatgcgc tggaacttac
     3541 cactgatgag caagatgatg acaaagaaat gctcgaaggt attataaaat ttggaaatat
     3601 cagggtggcg gaaattatga cttcgcgagt ggatatgata gctgtagata caaagacaaa
     3661 tttcaaggat ttattgaaaa ttatcatcca aacgggttat tctcgtattc ctgtttattc
     3721 tgaaaacaga gacaatataa agggcattct ttacagtaag gatttgctgc cccatcttga
     3781 caaacctgcc aatttcagat ggcaaagttt aattaggcaa gcttactacg tacccgaaac
     3841 aaaaaaaatt gacgatctgc tcagcgaatt tcagaaaaac aaagttcatc ttgctttggt
     3901 ggtcgacgaa tatggtggaa tttccggact ggttacgctg gaagatattt tggaagaaat
     3961 tgttggcgac atcagtgatg aatacgacga cgaagaaaaa ctttatacca aagtggacaa
     4021 tcacacctat atttttgaag cgaaaatttt gctgaacgac ttttttaaag ttacgggtat
     4081 caacgaagag gaattttcaa aagttactga agaagtggaa actttggccg gcttggtact
     4141 tgaactgaag ggagaaatgc ctgcaaaaaa tgaaatgatc gaatatggac gttacgtttt
     4201 tgaaatcttg tcggtggaca accgcagaat ccgaaaaatt aaacttcata tgaaagataa
     4261 tttcgatcgc aacaaagaag aaaattaaaa caactttttg atttgaatat caagctgttt
     4321 ttttgatgaa aaaacaatcg gcaacttcat ggaaacaaag aaaatcaaat acttgcaaac
     4381 aatattttat aaaacgacgc tccttatcgt tatcacggct tctttgttct cctgcggaaa
     4441 aaattatgtt ccccgtccct atggatattt cagggtagat ttacctcctc acaattatcg
     4501 ttatctcgat acgcttcaac taccttaccg atttgaaatt tcgagctacg ccaaagtgga
     4561 aaaaagagaa gctgaaggag aaaaatgctg gatcgatata aaatatcctt ttctgaatgc
     4621 ggccatttac tgcagttaca aacccattca tggaaatttg aaggaattgt tggaggatac
     4681 acgacaaatg gcttataaac acagcattcg tgccgatgcc attaatgaga aactttatca
     4741 gcatcccgaa aaaaaagttt acggaattct ttacgacttg aaaggaaata ccgcttcttc
     4801 ggttcagttt tttgtaaccg acagcattca tcactttttc aggggctcac tttatttcga
     4861 caatgtaccc aacaaggatt ccatagctcc catgtcggat tacattcggc aagacattat
     4921 caggttgatg gaaagttttg aatggaaaca atgattcaca gaaaaattga aacatccggc
     4981 gcaaaaattc tggtttggca aagtactgaa acgttcgacg agctggtgac tttctttgat
     5041 aaaaacgaaa ctgaagagtt aaacacctta cactccgaga aacgaaaaat cgaatatctt
     5101 tcggtacgtt tagcattgaa aactttgctg aacagagaaa taaaaatctg ttacaacgac
     5161 gatggcaaac cttttctttc ggattatttt gccgaaatcg gtatttccca cagtggtcaa
     5221 tggatcgccg tgataacaca tcctacttgc aaagttggaa ttgatattga acgctttcaa
     5281 cccaaaatat cggcaatttc caagcgattt ttgagcaaag aggaattgga gaaaatcttt
     5341 gaaaaagatg aaaacagact taaaaaactg accttgttgt ggtcggcaaa ggaatcgctg
     5401 tttaaagtta ttggaaaaga agcagtcgat ttttctcgtc agttgtgtat tcttccattc
     5461 gaaatgggaa aaaccggaaa aatcaaagcc ttgcacattc ctcgtaataa gttgtatgaa
     5521 ctcaattacg aattatcgga agaatatgca atggtttatc ttattgataa aaatgttgag
     5581 ccaaacaaat tatgaaaatt tatcacgata aaggataaaa gacaaaaatc atacacaaag
     5641 agaatcatga aaaatttgta caaacaaata ttgataaatc gggaatacaa caaaaaaatg
     5701 ctggccgttt tgctcgatcc ggatcgttgt cagggaaaac tgatgtcgtc aaccattgct
     5761 gcattaaaac tttcggctcc cgattttgta tttgtaggtg gcagttacgt caattctccg
     5821 atagattcgt ttattgagct tctccatgaa gaagtacaaa ttcctgttgt actttttccg
     5881 ggcaatgctt cccaattttc tccgaaagcc gatgcattgc ttttcctttc actaatttcg
     5941 ggcagaaatc ctgaattttt aatcggacag catgtaacct cggcgcttgc tatcaaacaa
     6001 tcgggaatcg aagtgattcc tgccggttac attcttatcg acgggggaaa agttagtgcc
     6061 gtagaataca tgagtcatac acgttccatt cctcgcgaaa aaaaggaaat tgttctggca
     6121 acagcggtgg ccggcgaatt gtcgggtcag catgttgttt atctggaagc cggcagcgga
     6181 gcaattcaac ccgttccaaa agaactgatt aaccatgtaa catctcacct ttccataccg
     6241 gtgattgtag gcggaggcat gaaaaccaca caacatctta atgactgctt cgacgcaggt
     6301 gccgacttgg ctgtcgtggg caacgctgtt gaacaaaatc ccgatttaat tgtcgaattt
     6361 accaattttg taaaaaatta taatacaata catcggtaaa taatgggtca gtaaagtggt
     6421 aattaataaa ttaaacaaaa atttttatga taaacttgta aaagagccca ttgagtttgc
     6481 tgcaattgct gtttgactct acattttatt tttaacccaa ctttccattc attaagcgga
     6541 actcattact aaacaagtaa atatactgtc tggcggatga atttgggtac tacaaatttt
     6601 tcgtagtttg aatggttgtt gtttgtactt tagctcttcg taaatttctg cagcttgagt
     6661 ttggggcata gtacaatatt ctaaaagcta cttgttctcc aagtttattt tcggcagtag
     6721 tggtcagcac tttttcagtt gacctgattc ttgttatttt ggtcataaag tacttgatgc
     6781 catgcctttt gagtctgttt ccaagaggtc actaattgat ctacccgctt tcgttggttg
     6841 gcatccaatt cgatgatgaa gcccgaaacc aagagaatac gactgcgaac tacttccgct
     6901 atatcgcggt acttttcctt gatacgaaaa taactcttgg gcatttttgt atccggtctg
     6961 tggcatactg ataaatcgaa atttattccg caaagttctg taattcaaat taaaccacca
     7021 aaatccaggg atactacaaa tgtgttctca gaaacaacaa ctattggaat ccaatatgtt
     7081 acaattttaa tcgactgaat ttgactatca aactaaccaa tatcagcctg tttttaacta
     7141 aactttaacg ttatgttatt catgtttgga aagttgggtt aatcttactt tcaaaaaaat
     7201 aaaaagaatt tcgcccaaag aatttcgtaa aaactctttg agcgcttagt aaccacgacc
     7261 attgatttgt ttcatcaatc aaggccattt gttctcaaat cttattttgc agcaaactca
     7321 gtaacagcaa aatgtctgga taaatccgtc gatttacttg atgcccagtt tagttttgat
     7381 ttcctttgga atagcattct tatgaaccaa aatattaatg gttttgtctt ttacaaatga
     7441 ttcgctggca taccagatac ctttgtattt gttatcggtt ccccacgaat tttttaccag
     7501 gtaatatttt gttccgttct gatctttggc aataccaaaa atcagcatcc cgtgatcgtc
     7561 ggtagtctga taattatcga atccacgctg acgactttcc tgtgttacct gcacttcggg
     7621 ctgaggtccc tgaattttgt aaattttttc ttccatttct ttgggtgaaa tattcagcca
     7681 ttttgcctga tccgaaccgg gcagcttttc cagtttt
//
LOCUS       DAOTAX010000074         7655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8160_length_7655_cov_29.234737, whole genome shotgun sequence.
ACCESSION   DAOTAX010000074 DAOTAX010000000
VERSION     DAOTAX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8160_length_7655_cov_29.234737"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..288
                     /locus_tag="PLM04_07340"
     CDS             121..288
                     /locus_tag="PLM04_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02923.1"
                     /translation="MSDNGDLLYKSKDGNLLQINHYDTRQLKLTVRYNFNTTKSKYKG
                     TEAGEKEMKRL"
     gene            complement(314..835)
                     /locus_tag="PLM04_07345"
     CDS             complement(314..835)
                     /locus_tag="PLM04_07345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HON02924.1"
                     /translation="MKFVRVLFSGLIVLCFLNGCRKTPPQLPANKNLDTTNNEVVALV
                     ELNEKMALKEDSVLKQFVKKLKGNFVKSPVGIWYFIENQTNGTLLENSDSCFISWQMF
                     SLEGKLLDEKSHEKVIIGKKQIPVGLEEGLRLMHKGESAVFVVPWYLAYGMKGLPPDV
                     PPYTSLIYKVKFE"
     gene            complement(839..3274)
                     /gene="thrA"
                     /locus_tag="PLM04_07350"
     CDS             complement(839..3274)
                     /gene="thrA"
                     /locus_tag="PLM04_07350"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HON02925.1"
                     /translation="MKVMKFGGTSVGSTEAVLNVKKIVESQKESVVVVVSALSGVTDQ
                     LYRISQLAASGNETYLDEYNQMFARHMQMIAETVLPEKQAEVELQIQKIFTDLLNIFR
                     GIFLIKDTSVKITDTVVSYGEMISSVLIANIIQGAVHFDSRRFIKTQNQFDKHIVDFK
                     TTNELIQQIFKDAPSIVVCGGFIATDVYTNQVTNLGRGGSDYTAAILAAELNASILEI
                     WTDVDGFMTADPRIINNAYVIDRLSYSEAMELSNFGAKVIYPPTIFPAFHRNIPIVIK
                     NTFRPEASGTYISQEKSGEKSKAIKGISSINDTSLITIRGLGMVGVIGVNYRIFRTLA
                     KNGISVFFVSQASSENTTSIGVKNADSALAVEVLKKEFANELELGEIDEIFSEDDLAT
                     VAIVGDNMKRTPGIAGKLFGTLGRNGINVVACAQGASETNISFVTDRSSLRKALNVIH
                     DSFFLSEYQVLNIFVVGIGKVGSHLLHQIKQQQPKLMHQKGLKLNVVGIASSKKMLFS
                     REGIDIEHYQDELKLHGVDSTPERIADEIINMNIFNSVFVDCTANAQIADLYQTLLDH
                     NISVVAANKIATSSSYDKYMKLKETARQKGIKFLFETNVGAGLPIISTMNNLTNSGDH
                     ILKIEAVLSGTLNFIFNTLSSDIPFSKAVLMAKEYGYSEPDPRIDLSGKDVVRKLVIL
                     AREAGYQVEQDDVKKNLFIPEKFFKGSLEQFWEQIPELDEAFELRRQELEKQGKRLRF
                     VASMDKGDCQVGLQEIDNTHPFYDLQGSNNIILLTTERYNEFPMMIKGYGAGASVTAA
                     GVFSDIISIANIR"
     gene            3429..3502
                     /locus_tag="PLM04_07355"
     tRNA            3429..3502
                     /locus_tag="PLM04_07355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3463..3465,aa:Arg,seq:tct)
     gene            3964..4653
                     /locus_tag="PLM04_07360"
     CDS             3964..4653
                     /locus_tag="PLM04_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HON02926.1"
                     /translation="MLIFVTPLHQYLPGYGDMNLRSKIIQESIRADSLTHAMNLQAAY
                     LEIVKDIIKGNIKPDSVVGVDSAFLQQQAQKLIETSKREKEFTKKFEEEEKFNLTSME
                     SSSTNKKTYVFFRPATGNIVSSFNISKKQYGVALQTLPDAAVSSVLNGIVVFADFTFD
                     NQWVIAVQHEDNYLSIYKNNNRLLKNIGEVVRTGEPIAITGSSKGKNGQLYFELWQQG
                     KPINPQNIIIF"
     gene            4699..5871
                     /locus_tag="PLM04_07365"
     CDS             4699..5871
                     /locus_tag="PLM04_07365"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HON02927.1"
                     /translation="MSNTTKQIAVLGSTGSIGKQALQVITDYPDRFSVYALTANNNID
                     LLIKQARKFQPEIAIIANENHFLQLKEALSDLPIKVFAGEESIAQLVEMQAVDIVLIA
                     LVGYAGLKPTMNAIKAGKRIALANKETLVVAGELIEEMVACYHPSIIPVDSEHSAIFQ
                     CLNGEGEKTEVEKIILTASGGPFRTKTSKELESVTIAEALKHPNWEMGAKITIDSASM
                     MNKGFEVMEAKWLFGVAPEQIEVVVHPQSIIHSMVQFIDGSIKAQLGVPDMRLPILYA
                     FTYPERISTSFPRIDFNLLNSLTFEKPDLERFHNLALAYYALDKGGNMPCALNAANEI
                     VVDAFLNSKLRFLQMSEVIETILSKTFYIGHPSYEDYVQTDIITRQITRELIAQVH"
     gene            5996..7486
                     /gene="rseP"
                     /locus_tag="PLM04_07370"
     CDS             5996..7486
                     /gene="rseP"
                     /locus_tag="PLM04_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HON02928.1"
                     /translation="METFLIRALQLILSLSILVVLHELGHFAFARLFKVRVEKFYIFF
                     NPGFSLLRAKKINGKWQFKFFSKNVPSNERPKKDAEGDIVMGEKGKPQTEPIPLSELS
                     DDDWRKYPESTEWGIGWLPLGGYCKISGMIDESMDTSQLKSDPQPWEYRSRPVWQRMP
                     IITGGVLVNFILAMVIYSAILFTWGKEYLPLENAKYGLHFTSVFIEQGFKNGDKILSV
                     DGKSCADLGEVIEKILIDNAKSVTVLRGDSQILTISLPPDFPQKVLASGESNLISYRF
                     PFVVQDVSPESPAKQAKLQPGDSIVGINGKELTIFQDVVSVLTNYRDSLIQLAYYRNG
                     QKEQVTIQLDQTGKLGVAVRPFTEFLETEHVKYGLLASIPAGISLGWETLTSYIKQFK
                     LVFTKEGSKQLGGFGSIGKLFPQTWNWQIFWSMTALLSVILAFMNFLPIPALDGGYVL
                     FLLYEIFTGKKPSDKFLEQAQTIGMLLLLALLIYANGNDIYKAIFK"
     gene            complement(7465..>7655)
                     /locus_tag="PLM04_07375"
     CDS             complement(7465..>7655)
                     /locus_tag="PLM04_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02929.1"
                     /translation="LLSDLQSPATPLPFANVSYQLGTYRNPHPASLIPCPTYKKETVS
                     TFETASLIFLFFYLKMAL"
BASE COUNT         2473 a         1438 c         1442 g         2302 t
ORIGIN      
        1 gcttttcacg gctcagttaa acttaaacct gcaattttga aggatggtct gttttgaaaa
       61 ttaaaacaaa aaagttatat tataggggtt acaagtataa gtatcgactg aataaaaatt
      121 ttgtcagata atggtgattt gctctacaaa agcaaagacg gtaatttgct tcaaatcaat
      181 cactacgata cacgtcaatt aaagttaact gtccgctaca atttcaatac gacaaaaagc
      241 aaatacaaag gaactgaggc cggtgagaaa gaaatgaaac gattgtagaa gtgtacagga
      301 aaatctttta ttattactca aattttactt tgtaaatcaa cgaagtataa ggagggacat
      361 cgggcggaag tcctttcatg ccataagcca aataccaggg aacaacaaaa acagcgcttt
      421 cgcctttatg catcaaacgc aatccttcct ccagaccaac aggaatttgc tttttcccga
      481 taattacttt ttcatgtgat ttttcgtcaa gtaactttcc ttcgagtgaa aacatttgcc
      541 aagaaatgaa gcaactgtcc gaattttcca gcagagtgcc attggtttga ttttctatga
      601 aataccaaat tccaacaggc gatttgacaa aatttccttt taatttctta acaaattgtt
      661 tcagcacact gtcttccttc aacgccattt tctcgtttaa ttcgaccaac gccacaactt
      721 cattattggt tgtgtccaaa tttttattgg cgggcaattg aggtggcgtt tttctgcaac
      781 cgttcaaaaa gcacaaaaca attaatccgg aaaataaaac acgaacgaat ttcattcttc
      841 aacgaatatt ggctatgctg ataatatccg aaaaaacacc tgctgccgta acacttgctc
      901 ctgccccata accttttatc atcatgggaa attcattata acgttccgta gtcagcagaa
      961 taatgttgtt gcttccctgc aagtcgtaga agggatgggt attgtcgatt tcctgaagtc
     1021 caacctgaca atcaccttta tccatagaag ccacaaaacg aaggcgtttc ccttgttttt
     1081 cgagttcctg ccggcgaagt tcaaaggctt catccagttc gggaatttgc tcccaaaatt
     1141 gctccaatga acctttaaaa aacttttccg gtataaacag attttttttc acatcatcct
     1201 gttccacctg ataaccggct tcacgagcca ggatcaccaa tttcctgacc acatccttcc
     1261 cgctcagatc gatacgcgga tcgggttcgg aatatccata ttcctttgcc atcaatacag
     1321 ctttactgaa aggaatatcg ctactcaaag tattaaaaat gaaattcaac gttccggaaa
     1381 gaacggcttc tatttttaag atatgatcgc cgctgttggt gaggttgttc atagtggaaa
     1441 taatgggtaa tcctgcaccg acattggttt caaaaagaaa ttttatacct ttctgacgag
     1501 cagtttcctt taatttcata tacttgtcgt aagaagatga agtggcaatt ttattggcag
     1561 ctacaaccga aatattatga tcaagcaaag tctggtacaa atcggcaatt tgtgcattgg
     1621 cagtacaatc cacaaaaacg gaattaaaaa tattcatatt gataatttcg tcggcaatac
     1681 gttcaggagt cgaatcaacg ccatgcaatt tcaattcgtc ctgataatgt tcaatatcaa
     1741 ttccttcgcg cgaaaaaagc atttttttgc tggaagcaat tccaaccaca ttcagtttca
     1801 agcctttctg gtgcatcaat ttaggctgtt gctgttttat ttgatgcaaa agatggctgc
     1861 ccaccttgcc aatacccacc acaaaaatat tcaacacctg atattcggac aggaaaaagg
     1921 aatcgtgaat gacgttcaaa gccttacgca acgaactgcg atcggtaaca aaagaaatat
     1981 tggtttcgga agcaccttgt gcacaagcaa ccacatttat tccatttcgt cccagcgtac
     2041 caaaaagttt tccggcaatt ccgggtgtac gtttcatatt gtcaccaact atggcaacgg
     2101 tcgccaagtc gtcttcagaa aaaatttcgt caatttctcc aagttccagt tcattggcaa
     2161 attctttttt cagtacttct actgccagcg cactatctgc attttttacc ccaatggaag
     2221 tcgtattttc agaagaagcc tgagaaacaa aaaatacact gataccgttt tttgccagtg
     2281 ttctgaaaat tcggtaattt acaccaatga cgcctaccat tcccaatcca cgaatggtaa
     2341 tgagcgaagt atcgttgatg gaagaaattc ctttaatggc ttttgatttt tcgccggatt
     2401 tttcctgaga aatataggtt ccgctggctt cggggcgaaa ggtatttttt ataacaatgg
     2461 gaatattcct gtgaaaagcc gggaaaatgg taggcggata aatcactttt gcaccaaaat
     2521 tgctcaattc cattgcttcg ctgtaactaa gacggtcgat tacgtaagca ttgttgataa
     2581 tacgtggatc ggcagtcata aatccatcaa catccgtcca aatttccaga atggaagcat
     2641 tcaactccgc agcaagaata gctgccgtat aatccgaacc tccccgaccc aaattagtta
     2701 cctgattggt atacacatcg gtagcaataa atccgccaca aactactatg gatggagcat
     2761 ccttaaaaat ctgctgaatg agttcattgg tagttttaaa atctacaatg tgtttatcga
     2821 actgattttg agttttaata aatcgacgcg aatcgaaatg aacagctccc tgaattatat
     2881 tggcaatcag aacggaggaa atcatttctc cataactgac aaccgtatcg gtgattttga
     2941 cggaagtatc cttaattaaa aagattcccc tgaaaatatt caacaaatcg gtaaaaattt
     3001 tctgtatttg cagttcaact tccgcctgct tttcaggcaa aaccgtttcg gcaatcattt
     3061 gcatgtgcct ggcaaacatt tggttatatt cgtcgagata ggtttcgttg ccggaagctg
     3121 ccagttggga aatacgataa agctgatcgg taacgcctga aagcgccgaa acaaccacta
     3181 ccacagattc tttctgcgat tcgacaattt tttttacgtt caaaaccgct tcggtagaac
     3241 ctacggatgt tccaccaaat ttcattactt tcattcgtca ggattttatt ttttcttcaa
     3301 aaaattttaa gttgcaatat tagtgatttt ttggcaaata aattgcacct taccacgatt
     3361 ttgagtaaaa gtgttgcatc ttaatcaaaa ttaaagtact tttgcatcgc ctttaacaaa
     3421 aggaatccgg tcccgtagct cagctggata gagcaacagc cttctaagct gtgggtcgtg
     3481 ggttcgaatc ccgccgggat cacgaaaaaa gcaggtaaaa ataacaataa attacctgct
     3541 ttctttttac aaaaaaaatc ggccatcatg gcaaagcaaa ctttttggag aataaatttt
     3601 ctaaaaaaac caaaaaacat gttttgagac aacatcatgc tttgttcgtt tcacaagatt
     3661 ccatgaatgg ggattcagag ttttatcaca aaactttttt acggcaggtt tacatgaatg
     3721 ggcaaaggag ttttatgtaa aatgaaaata atctcctatt tttgtattta ttttgtgagc
     3781 gattgaactt caagaatgaa aagaaaaaaa tttttctctt tttttcaaaa aatccggttt
     3841 aaatatcgtg tttccatatt aaatgaaaac acactcgaag aatcgtggca tattcattta
     3901 tcgaggttga gtgtttttat atatcttttt gtttttacta taataacttt tgcgttgttg
     3961 tctgtgttga tatttgtaac accattgcat caatatttgc ccggttatgg agatatgaac
     4021 ttgcgatcaa aaattattca ggaatcgatc cgagccgatt ctttaactca tgcaatgaat
     4081 ttgcaggcgg catatctgga aattgttaaa gatattatta agggaaatat taaacccgac
     4141 tctgttgttg gcgttgattc tgcttttttg cagcaacaag ctcaaaaatt aatagagaca
     4201 tcgaaaagag aaaaagaatt tacaaaaaaa tttgaagaag aagaaaagtt taatttgaca
     4261 agcatggaaa gttcttcgac caataagaaa acctacgtat ttttccgtcc tgcaacaggt
     4321 aatattgttt cctcattcaa tatttccaaa aaacaatacg gggtagcctt acaaacttta
     4381 cctgatgctg ctgtgtcaag tgttttaaat ggaatcgtag tatttgcaga ttttactttt
     4441 gacaaccaat gggtaatagc agttcaacat gaagacaatt atctttcaat ttataagaac
     4501 aacaatcgtt tactaaaaaa cataggcgag gtagtgagga caggcgaacc gatagccatc
     4561 actggttcgt ccaaaggaaa aaacggacaa ttgtatttcg agttgtggca acaaggtaaa
     4621 cccattaacc cacaaaatat cataattttt taaactacca gatttttatt cctaaaaaca
     4681 taaacaaatt gaaataaagt gtccaacaca actaaacaaa tagcagtttt aggctccaca
     4741 ggatctattg ggaagcaagc cttgcaagtg ataaccgatt atcccgacag gtttagtgtt
     4801 tatgcactca cagccaataa taatatagac ttacttataa aacaggctcg caaatttcaa
     4861 ccagaaattg ctattatagc aaacgagaac cattttctgc agctgaaaga agctcttagc
     4921 gaccttccca taaaagtttt tgctggagaa gaatccattg cgcagctggt agaaatgcag
     4981 gcagtggata tcgtattaat agctttggtt ggttacgcag ggctaaaacc aaccatgaat
     5041 gccataaagg caggcaagag gattgcattg gcaaataaag aaacattggt tgtagccggc
     5101 gaactaatcg aagaaatggt ggcttgctac catccttcaa ttatacccgt cgactctgaa
     5161 cattcagcta tttttcaatg tttaaatggg gaaggggaaa aaacggaagt tgaaaaaatt
     5221 atattgactg cctccggtgg acctttccgt accaaaacca gcaaagagtt ggaatcggta
     5281 accattgcag aagccttgaa acatcctaat tgggaaatgg gagcaaaaat aactattgat
     5341 tcagcctcca tgatgaataa aggttttgaa gtaatggaag caaaatggtt gtttggtgtt
     5401 gcaccggagc aaatcgaagt ggtagtacac ccacaatcca taatacattc aatggttcaa
     5461 ttcattgatg gatcgataaa agctcaattg ggagttcccg atatgcgttt gcccatcttg
     5521 tatgctttta cataccctga aagaatttca accagttttc cacgtataga ttttaacctg
     5581 ttaaacagtt tgacattcga aaaacccgat ttggagcggt ttcacaatct tgctcttgcc
     5641 tattatgctt tagacaaagg agggaatatg ccttgcgcat taaacgcagc aaacgaaata
     5701 gtggttgatg catttttaaa cagcaaactt agatttttac aaatgagcga agtcattgaa
     5761 actattttat caaaaacttt ttatataggt catccctctt atgaagatta tgtacaaaca
     5821 gatataataa ccagacaaat aaccagagag ttaattgcac aagtacattg aaacttcaga
     5881 tgagagaaaa acaaaaattt aatttataag taaacatttt aataatttag acgataaaca
     5941 agaaaattcg catgttgaaa ttcgttatcg gatgaagttg aatatttaga tcaatatgga
     6001 aacattttta attagagctt tacaacttat acttagtttg tcaattttag tagtactgca
     6061 tgaattgggg cattttgcct ttgcccgttt gtttaaagtt cgtgtggaaa agttttatat
     6121 tttctttaat ccgggtttct ctctattacg agcaaaaaaa attaatggca aatggcaatt
     6181 taaatttttt tcgaaaaatg tcccttcgaa tgagcgtccc aaaaaagatg ccgaaggaga
     6241 tatcgtaatg ggagaaaagg ggaaacctca aactgaacct ataccgcttt cagagctttc
     6301 agatgatgat tggagaaagt atcccgaatc aactgaatgg ggaataggct ggttaccttt
     6361 agggggttac tgcaaaattt cgggtatgat tgatgaatca atggatacat cacaactaaa
     6421 atccgatcca cagccatggg aatatcgttc acgtccggtt tggcaacgaa tgcctattat
     6481 tactggcgga gtattggtta attttatcct tgcaatggta atatattccg ccattctttt
     6541 tacttggggc aaagaatatc tgcctttgga aaatgctaaa tacggcctac attttacatc
     6601 ggtctttatt gagcaaggat tcaaaaatgg agataaaatt ttatcggtcg acggtaaatc
     6661 atgtgctgat ttaggagagg taattgaaaa aatactgatt gacaatgcca aaagtgttac
     6721 tgttctaaga ggcgacagcc aaattttgac tatttcattg ccccccgatt tccctcaaaa
     6781 agttttggct tcaggagaaa gcaatctgat ttcgtatcga ttcccattcg tggtacaaga
     6841 tgtttcaccg gaatcacctg caaaacaggc aaaacttcaa ccgggcgata gcattgtagg
     6901 tataaacggt aaagaattaa cgattttcca agatgtggta agtgttttaa ccaattatcg
     6961 agacagttta atacaacttg cctattatcg aaatggacaa aaagaacagg taaccattca
     7021 gcttgatcaa acaggtaaat tgggtgtcgc tgttcgtcct tttaccgaat ttttggaaac
     7081 cgaacacgtg aaatacggat tgctggcttc catacctgcc ggtatttctc tgggttggga
     7141 aacgttaacc agttatataa aacaatttaa attagtattt accaaagaag gttctaaaca
     7201 attgggtgga ttcgggtcaa ttgggaaatt gtttccacaa acatggaatt ggcaaatttt
     7261 ttggtctatg acagccctgc tttcagtcat tctggctttt atgaactttt tgcctattcc
     7321 tgctttggat ggaggctatg tgttatttct tctgtatgaa atatttacgg ggaaaaagcc
     7381 aagtgacaaa tttttagagc aagcccaaac tataggaatg cttctgttat tggctttgtt
     7441 gatttatgcc aacggaaacg acatttataa agccattttt aaataaaaaa atagaaaaat
     7501 cagggaggct gtctcaaaag tagagacagt ctcttttttg tatgtcgggc atggtattaa
     7561 gcttgcagga tgcgggttcc tgtatgtgcc gagttgataa gaaacattag cgaatggcaa
     7621 gggtgttgcg ggtgactgca aatctgaaag aagcc
//
LOCUS       DAOTAX010000075         7595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8236_length_7595_cov_29.542706, whole genome shotgun sequence.
ACCESSION   DAOTAX010000075 DAOTAX010000000
VERSION     DAOTAX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8236_length_7595_cov_29.542706"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..2035)
                     /locus_tag="PLM04_07380"
     CDS             complement(11..2035)
                     /locus_tag="PLM04_07380"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HON02930.1"
                     /translation="MNSIELLNKAADNIRILAAAMVEKAKSGHPGGAMGGADFMNVLF
                     SEFLEYDPENPTWESRDRFFLDPGHMSTMLYSVLAFSGKFTLDELAQFRQWGSPTPGH
                     PELDVMRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGDVMNQTIYAYISDGGVQEEI
                     SQGAGRIAGHLGLDNLIMFYDSNNIQLSTETKAVTSENTAEKYKAWGWKVIEIDGNDA
                     AQIRQALTEAKAEKERPTLIIGKTVMGKGIRKADGSSFENQTSTHGMPISECGASFEE
                     TIRHLGGDPEHPFVIFEETKALYEKRKNELKEIVARKKTAKEAWAKEHPDLAKKLESF
                     FSGKAPEVDWEAVVQQPNKSTRAASATVLGELAKQVENMITASADLSNSDKTDGFLKK
                     TKAFSKNDFSGSFLQAGVSELTMACLCIGMSLHGGVVPACGTFFVFSDYMKPAVRMAA
                     LMEQPVKFIWSHDAFRVGEDGPTHQPVEQEAQIRLMEKLKNHHGQNSMLVLRPADVEE
                     TTVAWKMALENIKTPTGLILSRQDIKDLPSTNGRKEEAKKAEKGAYVVQCDGNPDIIL
                     LASGSEVSTLNEAAVLLRNDGIRVRIVSVPSEGLFRLQSKEYQEMVLPQEVKKFGLTA
                     GLPVTLQGLINNNGKVFGMESFGFSAPYKVLDQKLGFTGENIYHQIKLML"
     gene            complement(2082..2786)
                     /locus_tag="PLM04_07385"
     CDS             complement(2082..2786)
                     /locus_tag="PLM04_07385"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HON02931.1"
                     /translation="MFQPSMQNIYRYFIISLFVAVAVNFSTAQNKTPASAKSPEVKWW
                     NGFLVKMDVASPVITGFSDTEHFGMEGSVQFNLKRKIFPVYEIGFGGADKLSSNEVRF
                     NTNGIFQRIGIDFGLLKQSSDKELINNMLTGGVRLGWSNFSYDLTNLSLSDDYWGGSQ
                     TLNFEGQSATKTWYEIVVGIQVQVYKNFYLGWNIRNRHFFKEDTSGNITPWYIPGYGK
                     TGSSNTGFNYTIGYHF"
     gene            complement(2740..3306)
                     /locus_tag="PLM04_07390"
     CDS             complement(2740..3306)
                     /locus_tag="PLM04_07390"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HON02932.1"
                     /translation="MIFINFKNLFDTLKNFLHNNFTKSTVFIWITAVLFFSTSCNVSD
                     EVCRKEKNSRLKVGFYTAVFNSSTGKYTSSSLAIDSLTVNGVDSLGNIVDSILYNNSK
                     NLSVIKLPLNKFSNESKFIIRFNDSIQDTLTVFHTNYDQYLSFECGMIRNHLIDTVLI
                     TNHFVDSVKIEEHNVSTIDAEHLQIFHN"
     gene            complement(3340..3858)
                     /locus_tag="PLM04_07395"
     CDS             complement(3340..3858)
                     /locus_tag="PLM04_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02933.1"
                     /translation="MAEILKLLIPALLVLLTAYLLLDRLLKNEEKRRNFELFKSNYSV
                     ITPIRLRAYERLMLVLERTIPNNLILSTIKPGMTNLELQSQLLNTIRHEFAHNLSQQI
                     YVSDELWNYISSAQESLLRLINMCASQCNPNEPASLLAEKILEIYAASEETPTEQAIR
                     KLKEEVRKYFPS"
     gene            complement(3887..4240)
                     /locus_tag="PLM04_07400"
     CDS             complement(3887..4240)
                     /locus_tag="PLM04_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HON02934.1"
                     /translation="MKKNENSILKIYASSTDKMGNELLYEHLVFLAKENGIAGVTVYR
                     GIMGYGLSSQKISTSRFWELTEKLPVVVEMMDTTDKLESFYQTIEEKLKNIPNGCLVT
                     LQPVEVKLHKFGNKK"
     gene            complement(4242..4616)
                     /gene="crcB"
                     /locus_tag="PLM04_07405"
     CDS             complement(4242..4616)
                     /gene="crcB"
                     /locus_tag="PLM04_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HON02935.1"
                     /translation="MKQLLIVGLGGFFGTMARYLVSRINLLENFLSLPLGTLTVNVSG
                     SFLIGILTGLASKSELLSADMRLFLMAGFCGGFTTFSSFTNENLQLIQNGQFLSAAVY
                     TVISLLLGFLAVFLGYAVTNFL"
     gene            complement(4687..5640)
                     /locus_tag="PLM04_07410"
     CDS             complement(4687..5640)
                     /locus_tag="PLM04_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON02936.1"
                     /translation="MDISVVVPLYNEEESIRPLYEWIERVMNENGFSYEVIFVNDGST
                     DRSWEIIESLKASSPEHVHGIKFRNNYGKSVALHFGFQAAKGDVVITMDADLQDSPDE
                     IPSLYDMIKNQNYDLVSGWKKKRYDAKLTKNFPSKIYNATARRVTKLQLHDMNCGLKA
                     YRNEVVKSIELYGEMHRYIPYLAKIAGFTKIGEKVVEHRKRQFGTTKFGWNRFVNGYL
                     DLMTLWFLSKFGKQPMHFFGLIGTLIFLLGLIAVIVVGAKKLIALSAHVSAPLVTSSP
                     YFYLAILAMILGTQLFLAGFVAELITRNSSERNNYLIEKEI"
     gene            complement(5765..6271)
                     /locus_tag="PLM04_07415"
     CDS             complement(5765..6271)
                     /locus_tag="PLM04_07415"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HON02937.1"
                     /translation="MQGNIFKSSMIYGLMMGILFSVNFIISIPQGAIFRIVSFFITIV
                     IVIVMYRYSVKYRDNECGGYITYGKSFSFIVLTFFFASIISAIVKFVYFQFINPEYLS
                     NVLQQSMKVVESMNLPLDDEYYTQMEKMMKPSVYTLQSIWVNVLLGALLALVMSAFVK
                     KDKDIFEE"
     gene            6620..7480
                     /locus_tag="PLM04_07420"
     CDS             6620..7480
                     /locus_tag="PLM04_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HON02938.1"
                     /translation="MDKTQKQILEAQRTELTEHLIYKKLADDCKDENNAKILYEIGQQ
                     ELEHAHYWEKKTGIKVKPDYARIRRTVFMARLLGLSFVLKQMEKREGTGSKLYDELSA
                     VYPETKLFSEQENAHEQQLLAMLDEERLQYVGSIVLGLNDALVELTGALAGFTLALGN
                     TRTISLAGLVTGISAALSMAASDYLSTKAEGDTRAAKSAAYTGVTYFLTVILLILPFL
                     LMPNKFGALALTLSIAVLIIFCFNYYISVAKNLSFKPRFIEMTTISLGVAAFSFFIGY
                     LLNQILGVNV"
BASE COUNT         2338 a         1560 c         1369 g         2328 t
ORIGIN      
        1 tttttcgctt ttaaagcatc aattttatct gatgataaat attttctccg gtaaatccaa
       61 gcttttgatc caaaacctta tatggagcag aaaagccgaa agattccatt ccgaatactt
      121 ttccattgtt gtttatcagc ccttgcaaag ttacgggcaa ccccgcagtg agtccgaatt
      181 tttttacttc ctgaggcaaa accatttcct gatactcttt tgactggaga cggaacaatc
      241 cttcggaagg aacggaaaca atccgcaccc gaattccatc attgcgaagt aaaacggcag
      301 cttcattcaa agtcgaaact tccgatccgg aagcaagcag aatgatatcg ggatttccgt
      361 cgcactgaac aacataggca cctttttcag cttttttggc ttcttcttta cgaccatttg
      421 tggacggaag atctttaata tcctgacgtg aaagtatcaa gccggtaggc gttttgatgt
      481 tttccagtgc cattttccaa gctactgttg tttcttcaac gtcagccgga cgaagaacca
      541 gcatggaatt ttgtccgtga tgatttttaa gtttttccat taagcgtatt tgtgcttcct
      601 gctccaccgg ctgatgagtt gggccatctt cacccacacg aaaagcatca tgcgaccaga
      661 taaacttcac cggttgctcc attaatgcag ccatgcgaac ggcaggcttc atgtagtcgg
      721 aaaatacgaa aaaggttccg caagccggaa caacaccgcc gtgtaaactc atgccgatac
      781 aaagacaagc catagtcaat tccgacacgc cggcctgtag aaatgaacct gaaaaatcgt
      841 tttttgaaaa agcttttgtt tttttcaaaa atccatctgt tttgtcggaa ttggaaagat
      901 cggcactggc agtaatcata ttctctactt gtttggccaa ttcgccaaga actgttgccg
      961 aagctgcacg agtggatttg ttaggttgtt gaacaacagc ctcccagtca acctcaggag
     1021 cttttcccga aaagaatgat tcgagttttt ttgccaaatc aggatgttct tttgcccaag
     1081 cttctttggc tgtttttttt ctggctacta tttctttcaa ttcgtttttg cgcttttcat
     1141 aaagtgcttt tgtctcttca aaaattacaa acggatgctc agggtcgcca cccaaatgtc
     1201 taatggtttc ttcaaaagaa gcaccacatt cactgatcgg cataccgtga gtagaagtct
     1261 gattttcgaa actactgccg tcagccttcc gaattccctt gcccatgacg gttttaccga
     1321 ttattaacgt aggccgctcc ttttctgctt tggcttccgt caaagcttga cgaatttgcg
     1381 cagcatcatt tccatctatt tcgataactt tccagcccca agctttgtat ttttcagccg
     1441 tattttcact ggtaactgcc tttgtctcgg tagaaagttg aatattattg gaatcgtaaa
     1501 acataatcag gttgtccaat cccaaatgtc cggcaattcg ccctgctccc tgtgaaattt
     1561 cttcctgaac accgccatcg gaaatatagg cataaatggt ttgattcatt acatcgccaa
     1621 agcgtgcttt taaaaatttt gctgcaatgg ctgctccaac agcataagta tgcccctgtc
     1681 ccagtggtcc ggaagtgttt tcgatgccgc gcattacatc caattcgggg tgacccggag
     1741 ttggacttcc ccattgacga aactgtgcca attcgtccaa tgtaaatttt ccggaaaaag
     1801 ccagaacgga atacaacatg gtggacatgt gtccgggatc aagaaaaaac ctatcgcgac
     1861 tttcccatgt aggattttca ggatcgtact ccagaaattc ggaaaaaagt acgttcataa
     1921 aatcggcacc gcccattgct ccgccgggat gacccgattt ggccttttct accatagctg
     1981 ctgccaaaat acgtatattg tcggctgctt tgtttaaaag ttcaattgaa ttcataataa
     2041 aaaaattttg ttgttaggtt gtgtttgtta ttcatgtttt tttagaagtg atatccgatg
     2101 gtataattaa agcctgtgtt ggaacttccg gttttcccat agcccggaat gtaccacgga
     2161 gtgatgttgc cggaagtgtc ttccttaaaa aaatgccggt tcctgatatt ccaccccagg
     2221 taaaaatttt tgtaaacctg tacctgtatt cctactacaa tctcgtacca cgttttggtt
     2281 gcggactgac cctcaaaatt taatgtttga ctgccacccc aataatcatc cgaaagggaa
     2341 aggttagtta aatcgtagct gaagttggac catcccaaac gaactccgcc cgtaagcata
     2401 ttgttaatta attctttatc actactttgt ttcaataaac cgaaatcaat gcctattctt
     2461 tgaaaaattc cgttggtatt aaaacggact tcattggatg aaagtttatc ggctccacca
     2521 aaacctattt cgtaaacagg aaaaattttt cttttcaaat tgaactgaac actcccctcc
     2581 atgccgaaat gttcggtatc ggaaaatcct gtaattaccg gagacgccac atccattttg
     2641 acaagaaacc cattccacca cttcacttca ggtgatttgg ccgatgccgg ggttttattc
     2701 tgtgcggtag aaaaatttac agccacagca acaaacaaac taattatgaa atatctgtaa
     2761 atgttctgca tcgatggttg aaacattatg ttcttcaatt tttaccgaat caacaaaatg
     2821 attggttatc agaaccgtat ctatcaaatg atttctgatc attccacatt caaacgacaa
     2881 atactggtca taattggtat ggaaaacggt aagtgtatcc tgaatgctat cgttgaaacg
     2941 aataataaat ttcgattcgt ttgaaaattt gttgagcggc aatttaatga cagaaagatt
     3001 tttagaattg ttgtaaagaa tagaatctac gatattgccg agagaatcaa ctccgtttac
     3061 cgtaaggctg tcgatggcaa gcgacgacga agtgtatttg cctgttgaac tgttgaaaac
     3121 agcagtataa aaaccaacct tgagacggga atttttttct tttcgacaaa cttcatcgga
     3181 aacattacat gatgtcgaaa aaaacaaaac agcagtaatc caaatgaaaa ctgttgattt
     3241 ggtaaagttg ttatgaagaa aatttttcaa agtgtcgaat aaattcttaa agttaataaa
     3301 aatcattgtt accgctttct cttttcaaag ttgtttgcat taggatggaa aatattttcg
     3361 cacttcttcc ttcaacttcc gaatagcttg ctcggtgggc gtttcttcac tggctgcata
     3421 aatttccaga attttttcgg ccagcaaaga agcaggctca ttcggattgc attgcgaagc
     3481 gcacatatta atcaaacgaa gtaaactttc ctgagctgaa gaaatataat tccaaagttc
     3541 atcactcacg taaatttgtt gcgaaagatt atgcgcaaat tcatggcgga tagtgttcaa
     3601 caattgcgac tgaagttcca agttggtcat gccgggtttt atggtgctta gtatcaaatt
     3661 atttggaatt gtacgctcca aaacaagcat taatctttcg taggctctca aacgaatggg
     3721 cgtaataaca gaataattgc ttttaaacaa ttcgaaattt cgccgttttt cttcgttttt
     3781 caaaagtctg tccagcaaaa ggtaagctgt cagcaaaacg agcagcgcag gaatcagcaa
     3841 tttcaatatt tcggccatga agattggtat gtttttagaa aatattctat tttttattcc
     3901 caaatttgtg cagtttcacc tcaaccggct gcagagtaac cagacaacca ttcggaatat
     3961 ttttcaattt ttcttctatc gtttgataaa agctctcaag tttgtcggtc gtatccatca
     4021 tttcaaccac aacggggagt ttttccgtca actcccaaaa acgcgaagtt gaaatttttt
     4081 gactgctcaa cccataaccc ataattccgc gataaaccgt tacgccggca atgccgtttt
     4141 cctttgccag aaaaaccaga tgttcgtaca gcagctcgtt tcccatttta tccgtactgc
     4201 tggcataaat tttcaatatg ctgttttcgt tttttttcat tttacaagaa attggttact
     4261 gcatagccaa gaaaaaccgc aagaaaacct aacaaaagac tgatgaccgt ataaacggct
     4321 gcactcagaa attgtccgtt ttgaatcagc tgcaaatttt cattggtaaa ggacgaaaaa
     4381 gtcgtaaaac ctccacaaaa tcctgccatc agaaacaatc gcatgtcagc cgataataat
     4441 tcacttttgc tggccaaacc cgttaaaatt ccaatcaaaa aactgccgga aacatttacc
     4501 gtgagcgtac ccaacggtaa ggataaaaaa ttttccagca aattgattct ggaaacaaga
     4561 tagcgagcca ttgttccgaa aaaaccacct aatccgacaa tcagcaattg cttcatattc
     4621 taaatatttc cttttttgca aggtactaaa aagctccctg tatccgagcc aatgaaagcc
     4681 attttcttaa atttcctttt cgatcagata gttgtttctt tccgacgaat ttcgtgtaat
     4741 cagttcggca acaaaacctg ccaggaagag ctgcgttcct aaaatcatag ccagaatggc
     4801 cagataaaaa tacggcgaac ttgtaaccag tggcgcagaa acatgtgccg acaaggcgat
     4861 aagtttttta gcacctacca caatcaccgc tatcaaaccc aacaggaaaa tcaacgtccc
     4921 tatcaggcca aaaaagtgca tgggctgctt cccgaatttt gacagaaacc aaagtgtcat
     4981 caaatcgaga tatccgttta caaaacgatt ccagccaaat ttggtcgttc cgaactgacg
     5041 cttacgatgt tccaccactt tttcgccaat ttttgtaaaa ccggcaattt tggcaagata
     5101 aggaatatat cgatgcattt cgccatacaa ttcgatactt tttaccactt cattgcgata
     5161 agctttcagt ccgcaattca tgtcgtgaag ttgaagtttc gtaacacgtc gagctgttgc
     5221 attatatatt ttagaaggaa aatttttggt aagtttggca tcataccgct tctttttcca
     5281 acccgaaacc aaatcataat tttgattttt tatcatatcg tacaaagacg gaatttcgtc
     5341 ggggctatcc tgcaaatccg catccatagt aatcaccaca tctcctttgg ctgcctgaaa
     5401 tccaaagtgc aaagctaccg atttaccgta attgtttctg aattttatcc cgtgaacatg
     5461 ctcgggagaa gaggctttca acgattcgat aatttcccat gaacgatcgg tacttccatc
     5521 attcacaaaa attacttcgt aactaaagcc attttcattc attactcgct caatccactc
     5581 gtaaagagga cgaatcgatt cttcttcgtt atataaaggt acaacaactg atatgtccat
     5641 ttttaaattg agttttataa attggctttt taaatcacta ccggatcaaa taatgcaatt
     5701 ttaaaaaaat cactcaagca tctgatataa aagaaacaat ttgataaaaa tcaatagaat
     5761 ttctttattc ttcaaaaata tccttatcct ttttcacaaa tgccgacata accagcgcca
     5821 gcaaagctcc cagcaatacg tttacccaaa tgctctgcaa agtataaacc gaaggtttca
     5881 tcattttttc catttgggta taatattcat cgtccaaagg caaattcata ctttctacta
     5941 ctttcatgct ttgctgcaaa acattggaaa gatattcggg attgataaac tgaaaataaa
     6001 caaatttaac aatggccgaa ataatggaag caaagaaaaa agtaagtaca ataaaggaaa
     6061 acgatttccc gtaagtgata taaccgccac actcgttatc gcgatacttg accgaatagc
     6121 gatacataac aatgacgatc acaatggtta taaaaaatga gacaattctg aaaatggcac
     6181 cctgagggat ggaaataatg aaattgacgg aaaaaagtat tcccatcatt aatccatata
     6241 tcatggaaga tttgaaaata tttccctgca tgattttatt ttttacattt actcatgcaa
     6301 aagtacaaaa aaagacctga atcggaaata ttaaattttt cagttcaatt ataagtcgag
     6361 aaaacaacac ttaaattcaa tttgtattag cccgtaataa ccgagcacat tgattgtatt
     6421 tttttttatt attttttact attcggtttt tttgagggct aatatagaac ataatgatga
     6481 ttttgttgtt aaagaaatat gatgtttatg cagaaaaata caatttggaa agagaattcc
     6541 aacaaaatta tataatctct aatgaggatt tttgttttaa ttttaatgat aagaaatcaa
     6601 attttagtac tcaaaaatta tggacaaaac acaaaagcaa attttagaag ctcaaaggac
     6661 tgaactgacc gagcatttga tttacaaaaa actcgccgat gattgtaaag atgagaataa
     6721 tgcaaaaatt ttgtatgaaa tcggacaaca ggaactcgag catgcccatt attgggaaaa
     6781 gaaaaccggt atcaaagtga aacctgatta tgccagaata cgtagaactg tatttatggc
     6841 gcgtttgttg ggactctctt ttgtgctgaa acaaatggaa aaaagagaag gaacgggttc
     6901 aaaattgtat gatgaattgt cggctgttta ccccgaaacc aaattgtttt ccgagcagga
     6961 aaatgctcat gaacaacaat tactggccat gctggatgaa gaaagactgc aatatgtcgg
     7021 ttctattgta ttgggattaa atgatgcgtt ggtagaattg actggagctt tggccggctt
     7081 tacgctggct ttgggaaata caagaactat ttcgctggca gggttagtta cgggaatttc
     7141 tgctgcgctt tctatggctg cttccgatta tctttctaca aaagccgaag gagatactcg
     7201 tgcagcaaaa tcggcagcat ataccggcgt tacctatttt ttgacggtaa ttttgctaat
     7261 ccttccattt ttgctgatgc ccaacaaatt tggagcctta gcgctaacgc tttcgattgc
     7321 cgttttaatt attttctgtt ttaattatta tatttctgta gcaaaaaatc tttctttcaa
     7381 gccacgtttc atcgaaatga caaccatcag cttgggcgtt gctgcttttt ctttctttat
     7441 cggttatctg ctgaatcaaa ttttaggggt aaatgtttaa aattcattta ctcgcttttt
     7501 gagataaacc gtaagtaaga ccatgtctat attcgtaaaa aaagggaatg cctcttctga
     7561 agcatcccct tttttaatag gttataaatt caatt
//
LOCUS       DAOTAX010000076         7534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8316_length_7534_cov_31.098275, whole genome shotgun sequence.
ACCESSION   DAOTAX010000076 DAOTAX010000000
VERSION     DAOTAX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8316_length_7534_cov_31.098275"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1150)
                     /gene="pheS"
                     /locus_tag="PLM04_07425"
     CDS             complement(131..1150)
                     /gene="pheS"
                     /locus_tag="PLM04_07425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HON02939.1"
                     /translation="MKDRILQLQEEISHLTAQTLEEVEALRIKYLSKKGEISQLFNDF
                     RNVSNEEKREIGQLLNELKNNAHDQINQLKEKLEANRGNVEKIDLTRTAEPIQIGTRH
                     PLSIVRNEIATIFSGIGFTIAEGPEIEDDWHVFEALNFPADHPARDMQDTFFIEKNPD
                     ILLRTHTSSVQTRVMTQQKPPIRMIFPGRVYRNEAISYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALIYFAKEMFGMDTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCPFCKYTGWV
                     EILGCGMVDPNVLENCGIDSKIYSGYAFGMGVERITNLKYQVKDLRMFSENDIRFLRQ
                     FESAY"
     gene            1362..2033
                     /locus_tag="PLM04_07430"
     CDS             1362..2033
                     /locus_tag="PLM04_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HON02940.1"
                     /translation="MTIQKILDFLSVLSKNNNREWFAQNKSWYDEVRSGYLDMSNSLI
                     MKIAEFDPEIKHVDIKDCIFRIYRDIRFSPDKSPYKTHFGAYIAAGGGRKSQRGGYYF
                     HLQPESSFVSVGIWSPEPNVLKALRKSVYENIDEFNDILSQPNFKRFFGGFFEDGKLK
                     KVPAGFPGDFPQADLLKLKHYATSSPLDGSILTDENPVPHIAEILEAGYPLNRFLNFT
                     LDELF"
     gene            2046..2492
                     /locus_tag="PLM04_07435"
     CDS             2046..2492
                     /locus_tag="PLM04_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HON02941.1"
                     /translation="MILDTLKNAELYESAHPLFKKAFDFLKTTDLKNLPLGKIELEGS
                     DLVVSVVDISGKTVQDARMEIHRKFIDIQLPLDGEEIMGWIPAEKLTKVLQPYDAEKD
                     IAFFADEAENLLKVRAMEFAVFFPSDGHQPGIGFGNHKKIIVKVRV"
     gene            2562..4562
                     /locus_tag="PLM04_07440"
     CDS             2562..4562
                     /locus_tag="PLM04_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HON02942.1"
                     /translation="MEILPIVKIDPWLEPYAGSIYGRYEYFLQTEKKLTGGKQSLSDF
                     ATGYLYFGLHRVADGWVFREWAPNATAIFLIGDFNNWQRHPDYQLKKLQNGIWEIHLP
                     EYAMGHTQLYKLLVEWEGGSGERIPAWAQRVVQDTQTKIFSAQVWNPFQPYEFKNRHF
                     KPQRDPLLIYECHIGMSSQEEKIATYDEFRRNVLPRIAALGYNAIQIMAIQEHPYYGS
                     FGYHVSSFFAPSSRFGTPEELKQLIDEAHGMGIAVIMDIVHSHAVRNEVEGLGRFDGT
                     PYQYFHGGPRREHPAWDSLCFDYGKPEVLHFLLSNCKYWLDVFHFDGFRFDGVTSMIY
                     LSHGLGEDFTSYDCYYNLNQDGDAICYLTLANKLVHQVNPNAISIAEEVSGMPGLAAK
                     IDDGGIGFDYRMAMNIPDFWIKIIKEEKDEDWKVGRIFWEMTNHRAEEMTISYAESHD
                     QALVGDKTIIFRLIDADMYWHMRRGDNTLTVDRGVALHKMIRLVTASTMNGGYLNFMG
                     NEFGHPEWIDFPREGNGWSYKYARRQWDLVDNKNYLYYCLNEFDRAMIMLIRSVPRFN
                     KLPIDQLWIKEDDQVLVFQRGKLVFVFNFNPSKSFTDYGILAEKGAYRTILNSDNSLF
                     GGYDLIDESIVHVTLPEPTDPGGKEWLKLYLPARTAFVLRKD"
     gene            complement(4660..5055)
                     /locus_tag="PLM04_07445"
     CDS             complement(4660..5055)
                     /locus_tag="PLM04_07445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HON02943.1"
                     /translation="MATIFSKIAAGEIPSYKIAENDLYFAFLDIHPLTKGHTLVIPKK
                     EEDYIFRLDDENYQGLMLFAKKIALAIEKAIPCKRIGMAVIGLEVPHAHVHLIPIQQE
                     NDMNFRNPKLELSSEEMDKIAASIRKYLE"
     gene            complement(5082..5555)
                     /gene="greA"
                     /locus_tag="PLM04_07450"
     CDS             complement(5082..5555)
                     /gene="greA"
                     /locus_tag="PLM04_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HON02944.1"
                     /translation="MATVTYMTAEGYKKLVDELHQMENVQRPAISRQIAEARDKGDLS
                     ENAEYDAAKEAQGMLEMKIAILKDTIASARLIDESKIDTSEVQILTKVTIKNTKTNQV
                     MTYTIVSESEANIRAGKLSVTTPIAKGLLGKKVGEKTSISVPNGVQEFEILDISL"
     gene            complement(5702..6064)
                     /locus_tag="PLM04_07455"
     CDS             complement(5702..6064)
                     /locus_tag="PLM04_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02945.1"
                     /translation="MKKKKKSDTPKPEEKKHYEQATWQELKPVVERREFEKVVKAPTS
                     KPVEEYMENTYATYDTVGNDISELRIKKPVKVSIENNHHLIEEDRYEESSSIPLELDS
                     PEAAKKAFIYSVIFERKY"
     gene            6495..>7534
                     /locus_tag="PLM04_07460"
     CDS             6495..>7534
                     /locus_tag="PLM04_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HON02946.1"
                     /translation="MKYGFFVVVFSIFAFFFGYVGFRGWQSLALYPSARPTYIVSFLL
                     LFICSMAGMIFGDDMPASIAKVLTFTGYSFLLIFAYLFIVFLLVDVVRIANHFFHFAQ
                     SNMVSFRFWSSIVGLSVIIIAMIIGNYKFNHPEVVQLELQSNKPFQNKKIRIVAASDI
                     HLGTYIDKNYLKKYVQFINDQRPNIILLAGDICDRSLKPLIHQKMYEELHQLKAPLGV
                     YAILGNHEYYSQNLQNTVLFYKDAGIVVLADSISFVNNDFYLVGRKDRTDKNRQPLST
                     LLTTLEQDKPIILLDHQPLHLVEAAKNNVDLQISGHTHEGQVFPANLLVKSLFENGYG
                     YLKKGNTHIYVSS"
BASE COUNT         2174 a         1377 c         1511 g         2472 t
ORIGIN      
        1 cgcaacaccc ttgccattcg ctaatgtttc ctatcaactc ggcacataca ggaacccgca
       61 tcctgcaagc ttaatgccat gtcagcaata ttcttaaaag cgctctctgt tttttctgaa
      121 tactatcaaa tcagtatgcc gattcgaatt gtcgcaggaa acggatgtca ttttcagaga
      181 acatgcgcaa atccttaacc tgatatttta aattggtaat tcgttcaact cccattccaa
      241 aggcataacc ggaataaatt tttgaatcga taccgcaatt ttcgagtaca ttgggatcta
      301 ccattccgca acccaatatt tctacccagc ctgtatattt gcaaaacgga caaccttttc
      361 cgccacaaag attgcacgaa atatccattt cggcgcttgg ttcggtaaaa gggaagtagg
      421 aaggacgcag ccggatttgt gtatccatgc caaacatttc ttttgcaaaa taaatgagtg
      481 cctgtttcaa atcggcaaac gacacatttt tatcgatata caaaccttct acctgatgaa
      541 agaaacaatg tgcgcgatac gagatagctt catttctgta aacacgtccc ggaaaaatca
      601 ttctgatggg cggtttttgc tgtgtcatca cacgggtttg aaccgaagaa gtatgagtgc
      661 gcaagagaat atctggattt ttttcgataa aaaatgtatc ctgcatgtct ctggcgggat
      721 gatcggcagg aaagttcaag gcttcaaaaa catgccagtc gtcttcaatt tccggtcctt
      781 cggcaatggt gaacccgata cctgaaaaaa tcgtcgcaat ttcgtttctg acaatggaaa
      841 gaggatgacg tgttcctatc tgaatcggtt cagcggtgcg cgtcaaatca atcttttcca
      901 catttccccg attggcttcc aatttttcct tgagctggtt gatttgatcg tgtgcattgt
      961 ttttcagttc gttgagcaat tgcccgattt cccgtttctc ttcattactt acatttctga
     1021 aatcgttgaa aagctgtgaa atttctccct ttttgctcag atacttgatt cttaaagctt
     1081 caacttcttc gagcgtttgg gctgtaagat ggctgatttc ttcctgcaat tgtagaattc
     1141 ggtctttcat tttttatatt ggaatttgca aaaaaattga ataagtcctg agaacagaaa
     1201 taacctgtta ttattttttt aaattaactg caaaagtaat tattttttac caaaatagga
     1261 ggaatttata ccggaaaaag gaattacttc aaaaaaatga ggataaatgg caaagattaa
     1321 ataaatttta cattatattt gtcgtgctta aaatataaat tatgaccata caaaaaatac
     1381 tcgattttct gagtgttttg agcaaaaaca ataaccgtga gtggtttgct caaaataaaa
     1441 gttggtatga tgaggtgcgt agcggttatt tggatatgag taattcactg ataatgaaaa
     1501 tagccgaatt tgatcccgaa atcaagcatg ttgacataaa agattgcatt ttccgaattt
     1561 acagggatat acgtttttct cccgataaat cgccatacaa aactcatttt ggtgcttaca
     1621 ttgctgcagg cggtggtaga aaaagtcagc ggggtggtta ttatttccat ttacaaccgg
     1681 aaagttcttt tgtttcagtg ggaatttgga gccctgagcc caatgtgctg aaagcgttac
     1741 gaaaaagtgt gtacgaaaat atcgacgaat ttaacgatat tctgtctcaa ccgaatttca
     1801 aacgtttttt tggcggtttt ttcgaagatg gaaaattaaa aaaagtaccg gcaggttttc
     1861 ctggtgattt tccgcaggcc gacttgctca aattaaagca ttatgcaact tcctcaccat
     1921 tggacggatc cattttaacg gatgaaaatc ctgttccgca tattgcagag attttggaag
     1981 ccggctatcc gctcaacagg tttttaaatt ttactttgga cgaattattt taaacacata
     2041 aagccatgat actcgatacc ttaaaaaatg ccgaattata cgaatcggca catccacttt
     2101 tcaaaaaagc tttcgatttt ctgaaaacaa ccgacttgaa aaatttgcct ctcggaaaaa
     2161 ttgagttgga gggcagtgat ttggttgtca gcgtggtgga tatttccggg aaaacggttc
     2221 aggatgcccg catggaaata catcggaagt ttatcgacat tcagcttcca cttgatggtg
     2281 aagaaataat gggctggatt cctgccgaaa agttgacaaa agttttacaa ccttacgatg
     2341 ctgaaaaaga tattgctttt tttgccgatg aggcagaaaa tctccttaaa gtgagagcga
     2401 tggaatttgc tgtttttttc ccttccgatg gtcatcaacc gggaattggc tttggaaacc
     2461 acaaaaaaat tatagttaaa gttcgtgttt aggagtacaa ttttatttcc aaccagaaaa
     2521 tttattttgt tttgcacaaa accacattaa gctaaaaaat tatggaaatt cttccaattg
     2581 ttaaaattga tccgtggctc gaaccttatg cgggttctat ttatggaaga tatgaatatt
     2641 ttcttcagac tgaaaaaaaa ctgacgggag gcaaacagag tttgtccgat tttgcaacag
     2701 gatatttata ttttggactt catagagtgg cggatggttg ggtttttcga gaatgggcac
     2761 ccaatgctac ggccattttt ttaattggcg atttcaataa ttggcaacgt catcccgact
     2821 atcagttaaa gaagttgcaa aacggaattt gggaaataca tttgcctgaa tacgcaatgg
     2881 gccatacaca actttacaaa cttttggtag aatgggaggg cggaagcggc gaaagaattc
     2941 cggcttgggc tcagcgtgtg gtacaggata cgcaaaccaa aattttcagt gcgcaggtgt
     3001 ggaatccttt ccaaccctat gagttcaaaa atcgacattt caaaccacaa agagacccgc
     3061 tgttgattta cgaatgccat atcggcatgt cgagccagga agaaaaaatt gctacttatg
     3121 acgaatttcg ccggaatgta cttcctcgca ttgctgcgct gggttacaat gccattcaaa
     3181 ttatggctat tcaggagcat ccttattacg gttcgttcgg ctatcatgtt tccagctttt
     3241 ttgctccttc atcgcgtttt ggtacgcctg aagaactcaa acaactgatt gatgaagctc
     3301 atggaatggg aattgccgtt atcatggata ttgttcattc tcatgcagtg agaaatgaag
     3361 tggaaggatt gggcagattt gacggaacgc cttatcagta ttttcacggg ggcccgcgac
     3421 gtgaacatcc tgcttgggat tctttgtgtt tcgactacgg caaacccgaa gttttacatt
     3481 tccttttgtc caattgcaaa tactggctgg atgtttttca tttcgacggt ttccgtttcg
     3541 atggcgtaac atcgatgatt tatctcagtc atgggcttgg tgaagatttt accagttacg
     3601 attgttatta taatctgaat caggatggcg atgccatttg ttatcttaca ctggccaaca
     3661 aactggttca tcaagtaaat cccaatgcca tctccattgc ggaagaagtg agtggaatgc
     3721 cgggattggc tgcaaaaatt gatgatggcg gaattggctt cgattaccga atggccatga
     3781 atattcccga tttctggatt aaaattatca aggaagaaaa agatgaagac tggaaagttg
     3841 gacggatttt ttgggaaatg accaatcatc gtgccgagga aatgaccatc agttatgccg
     3901 aatcgcacga tcaggctttg gttggcgaca aaacgattat tttcagattg atagatgccg
     3961 atatgtattg gcacatgcga cgtggcgaca acactttgac tgtcgatcgc ggagtggcgc
     4021 tgcacaaaat gattcgtctg gttacggctt caaccatgaa tggtggttat ctgaatttta
     4081 tggggaacga gttcggacat cctgaatgga tcgattttcc gcgcgaaggc aacggttggt
     4141 catacaagta tgcccggcgt cagtgggatt tggtggacaa caagaattat ctatattatt
     4201 gtttgaacga atttgacaga gccatgatta tgctgattcg ttccgtgcca agatttaata
     4261 aacttcccat tgaccagtta tggataaaag aagacgatca ggtgctggtt tttcaaaggg
     4321 gaaaattggt gttcgtattt aattttaatc cttcaaaatc gtttaccgac tacggtattt
     4381 tggcggaaaa aggtgcttac agaaccattt taaattccga caattcctta tttggaggtt
     4441 atgatctgat tgatgaaagt attgtgcatg ttaccttgcc cgagccaaca gatcccggcg
     4501 gaaaagaatg gctgaaatta tatctccccg cccgaacagc ttttgttttg agaaaagatt
     4561 gaaaagtttt ttgatttgga gctactccat ttgaatacga caccccaaaa gcttcgagta
     4621 acttttgggg tgttgcatgt aaagttggaa caaggaaact cactccaaat attttcggat
     4681 agaagctgca attttgtcca tttcttcgga tgacaattcc aatttcgggt ttctgaaatt
     4741 catgtcgttc tcctgttgaa tgggaataag atgaacgtga gcatgaggca cttccagccc
     4801 aatgactgcc attcctatcc gtttgcaggg aatggctttt tcgatggcaa gggcaatttt
     4861 ttttgcaaaa agcatcaatc cctgatagtt ttcatcgtcc aagcgaaaaa tgtaatcttc
     4921 ttcttttttg ggaatcacca aagtatgtcc ttttgtcaaa ggatgaatgt cgaggaaagc
     4981 aaaataaagg tcgttttccg caattttata actgggaatt tctccggctg caattttact
     5041 gaaaatggtt gccatgattt taaaagttta actttccgtt attatagtga aatatcgaga
     5101 atttcaaatt cctgtacgcc attgggtact gaaatggaag ttttttcgcc cactttttta
     5161 cccagcaaac ctttggctat cggagttgta accgataatt tgcctgctct gatattggcc
     5221 tcactctctg aaacgatggt gtaggtcatt acctgattgg ttttggtatt ttttatggtt
     5281 actttggtta aaatctgcac ttcactggta tcgatttttg attcgtcaat caaacgtgcc
     5341 gatgcaatgg tgtccttgag aatggcaatt ttcatttcga gcattccctg agcttctttt
     5401 gctgcatcgt attcggcatt ttccgacaaa tcgcctttgt cgcgagcttc agctatttgc
     5461 ctcgagatgg ccggacgctg gacgttttcc atttgatgca gttcatccac cagtttctta
     5521 taaccttcag cagtcatgta tgttactgtt gccatagttg tatttttttt agtttgaata
     5581 aaaatataaa aacaaaaaga attccgaatc atgtaaagat cggaactctc tctgttatgt
     5641 ttttcgatgt ttatttgact acaaatatag tattttttat gataacaaaa aaattttgat
     5701 tttaatattt tctttcaaaa attaccgagt aaataaaagc tttttttgca gcttccggtg
     5761 aatccaattc caaaggaatt gagctggatt cttcgtagcg atcctcctct atgagatgat
     5821 ggttattttc aatggaaact ttgacgggtt ttttgattcg gagttctgaa atatcgttgc
     5881 cgacggtatc gtatgttgca taagtatttt ccatgtactc ttcaaccggc ttgctggtag
     5941 gagcttttac caccttttca aattcccttc tttcaactac gggcttaagc tcttgccaag
     6001 tcgcttgctc gtaatgtttt ttttcttccg gtttcggtgt atcgcttttt ttcttcttct
     6061 tcatattgct cagtagcacc acgactacga aaataagaaa tatgtaattg ccaaagtctt
     6121 ccataattta ttgttgatgt tattgaaaag caaatttaca gtaaatcttt gaatatttta
     6181 aaaaaaatag agaaaaaatc gatttattta aaacatcaat ttcatcagaa tataatttat
     6241 ttttttaaag gtctgatgga gcgcacatgt ctgaatatta tattcattcg tgtttgtgta
     6301 tgtaatacct gtgggtttca taattaatta tcaaaaaact attttctcgg tatagggtca
     6361 tgaacaattt tcgtaaaaat acgtttttat gagcaattga taattcaaaa tttaaagtgt
     6421 cctatttaat tttttttgaa ccaaagccgc taaaagtaaa cgcacagctt tgattaatta
     6481 aaccaaaata tattatgaag tacggatttt ttgttgttgt tttttctatt tttgcgtttt
     6541 tttttggata tgtcggtttt cgtggttggc aatcgctggc cttgtatcca tcagcacgac
     6601 ctacctatat agtcagtttt cttttgttgt ttatttgttc aatggctggc atgattttcg
     6661 gcgatgacat gccggcatca attgccaaag ttttaacatt tactggatat tcgtttcttc
     6721 tgatttttgc ttatttattc attgtttttt tgttggttga tgtggttcga atagccaatc
     6781 atttttttca ttttgctcaa agcaatatgg tttctttcag attctggagc tcaattgttg
     6841 gtttatcggt aattataata gccatgatta tagggaatta caagtttaac catcctgaag
     6901 tagttcaact cgaattgcaa agtaataaac cttttcaaaa taaaaaaatc agaattgtgg
     6961 ctgccagtga tatacatttg ggcacttata tcgataaaaa ttatctaaaa aaatatgttc
     7021 aattcatcaa tgatcagcga ccgaatataa ttttgttggc aggcgatatt tgtgatcgct
     7081 ccctcaaacc tttgattcat caaaaaatgt acgaagaatt gcaccaactg aaagctcctt
     7141 taggtgtcta tgccattctg ggcaaccacg agtattattc tcagaatttg caaaatacgg
     7201 ttttatttta taaagatgcc ggaattgtag tactcgccga tagcatttca tttgtcaaca
     7261 atgattttta tcttgtagga cgaaaggaca gaaccgataa aaacaggcaa cccctttcaa
     7321 cattgttgac aacgcttgaa caagataagc cgataatctt acttgatcat cagccgttgc
     7381 atttagtaga agcagcaaaa aacaatgttg atttgcaaat ttcgggacat actcacgaag
     7441 gacaggtttt ccctgccaat ttgcttgtaa aatctttatt tgaaaacggc tatggatatt
     7501 tgaaaaaagg aaacactcat atttatgttt cgtc
//
LOCUS       DAOTAX010000077         7368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8563_length_7368_cov_30.893614, whole genome shotgun sequence.
ACCESSION   DAOTAX010000077 DAOTAX010000000
VERSION     DAOTAX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_7368_cov_30.893614"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PLM04_07465"
     CDS             <1..167
                     /locus_tag="PLM04_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tat (twin-arginine translocation) pathway signal
                     sequence"
                     /protein_id="HON02947.1"
                     /translation="FMSQDIVAVDTAATNFFNQVRDMPLESVGHLVKAQELKVGTMNL
                     NQLNIKRIKM"
     gene            174..1700
                     /locus_tag="PLM04_07470"
     CDS             174..1700
                     /locus_tag="PLM04_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HON02948.1"
                     /translation="MLKKLRVVVSLLFFIPITLYFLDFRGVLPQQFHWLAKIQLVPAL
                     LSLNLIILTIIAGITFIFGRIYCSSVCPLGIFQDIVSWVSKQINKKKKFKFSTAKNIL
                     RWSVLGVIVITFFLGFSFLTGFFDPYSIYGRMATHLFKPVYMEGNNLLAGIFNAAGFH
                     SLYKVQIYLLSLGTFLITLAMMLIIGYLSWKNGRLYCNTICPVGTTLGFLSKYSLFKI
                     RFDQEQCNSCGLCSMNCKSSCIDAKEKTIDYSRCVNCFNCMEACKRNGFKFSTKSALR
                     RKSTDNQTTTDIDNNKRRFLSTLAVSSFAVAGLPQEGIKINGFHKVKRETPVSPPGSK
                     SAENLMSACTSCHLCVSKCPSQIIRPSLFEYGLGGIMQPMLSFEKGFCNYDCTVCSEI
                     CPSGALSALTKEEKHKNQMGQVHFILENCIVYKDETSCGACSEHCPTQAVSMVPYKNE
                     LTIPHTDTGICVGCGACEYACPAFPDKAIYVEGLKVHQQAAIKKEKSNHKIEIEDFGF
                     "
     gene            1736..1930
                     /gene="tatA"
                     /locus_tag="PLM04_07475"
     CDS             1736..1930
                     /gene="tatA"
                     /locus_tag="PLM04_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HON02949.1"
                     /translation="MTLTTILLAIGTPEILIIALVILLLFGGRKIPELMRGLGKGVSQ
                     FKKGMKDVEDEIKEDDNKKE"
     gene            2058..2864
                     /gene="tatC"
                     /locus_tag="PLM04_07480"
     CDS             2058..2864
                     /gene="tatC"
                     /locus_tag="PLM04_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HON02950.1"
                     /translation="MSLNNKANEQETTFWWHLEKLRWVILRTLAVVGLLMVLFFSNKD
                     FLFNKIILAPLNSDFYVYRWFNEIAKLLNFSDLTIDSFHLQLININLAGQFMAHLSIS
                     FAFALIVSVPYLLFEIWRFIFPALYPHERKNIELIFFFSSLLFYLGAIVSYLIIFPLT
                     IRFLGTYQVSSLVPNQIALSSYISALYILVFSLGLTFEIPVLSYFLSRLGIITRQMLK
                     KGRGLALVIILIAAAVITPTTDPFTMMVVTVPLYLLYEVSILVCKKDTGQ"
     gene            3270..3647
                     /locus_tag="PLM04_07485"
     CDS             3270..3647
                     /locus_tag="PLM04_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HON02951.1"
                     /translation="MKKVIHTSDAPAAIGPYSQAVESSNILFISGQIPICPQEGKIIS
                     TTIDGQTEQVFSNIEAILKSAGYSFGQIVKTTVFLTDMSHFAAMNEIYKRFFTEDCPA
                     RSTIAVKELPQGALVEIETIAMK"
     gene            complement(3652..4608)
                     /gene="fmt"
                     /locus_tag="PLM04_07490"
     CDS             complement(3652..4608)
                     /gene="fmt"
                     /locus_tag="PLM04_07490"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HON02952.1"
                     /translation="MRKSKEELRIVFMGTPEFAVESLKILVEGGYNVVGVVTMPDKPA
                     GRGYKVQFSPVKQYALENNLLLLQPEKLKDEIFLENLQSLKADLQIVVAFRMLPEIVW
                     NMPPLGTFNLHASLLPQYRGAAPINWAIINGEKETGVTTFFLTHEIDTGKIILQEKIP
                     ILPSDNAGTLHDKLMMLGASTVKKTVDLIISDAVQPIDQSELLKNTIELRSAPKIFRE
                     NCEIDVSWDVERAHNFVRGLSPYPGAWMNLVFPHTNEKTVLKIFQAEKEFSSHQLPLG
                     SLKTDGKKFAKIALKDGFLVLKEVQAPNKKRMEIGEFLRGLR"
     gene            4864..7161
                     /locus_tag="PLM04_07495"
     CDS             4864..7161
                     /locus_tag="PLM04_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HON02953.1"
                     /translation="MKKLYLLSFAFILCFSLSSTAQKVGLVLSGGGAKGVAHIGVIKA
                     LEENGIPIDYIAGTSMGAIVGSMYAMGLTPEEMLNIIKSDDFRFWLTGQNEPEYVFYF
                     RNGNVKPTFMEISFRFNPHDTVRVKSNVLPTHIVPTHQLNYAFMALFSQATAASEQNF
                     DRLFVPFRCVASDIEEKVPVVFSKGSLGDAVRASMTFPLVFKPIVIDNKLLFDGGIYN
                     NFPVDVMRNDFNPDIMIGSVVAKNPQKPTEDNLLLQIENMVMSQTDYVIPKEEGFMLR
                     FDLKNFNMFDFTKADQLYQMGYDSTLQKIEEIKSAISRRISEEELNKNRQDFRDKFPE
                     LKFQNVYIEGIDSLQKLYVEREFHTGNEVFDLKSFKKSYFNLLSDNQISEITPAAVYN
                     KTSGLFDLHLKVKTEDHLKVMMGADISSMASNQAYIGLSYQNLKEYAQTAYVDAQFGR
                     MYNALGVGVRMDAAAKTDFYVKFGFVTHKFDYFDGISLFYNDNRVSYFNQQETYGKLS
                     IGFPLTKKGRIEFGLGGGYLADHYFQNKSLITSGVKNDKSSYSLGSVFVDAESNTLNR
                     PNYPTGGSYYAASLRLTGGNHNEYLNYPVVRELEGQELWLQFLGKYDRYINLSKNVTL
                     GTYGELAISNRKLLPNYTATMIQSPAFRPTLHSTTIFNEGYNANQYAAFGLKPIYSFN
                     NQLHFRTEAYCFVPYKTIYRLPDESAAYSKPFSDVEFISEAAMVLSFKKATLSAFVNY
                     YSAGSNHWNVGFNIGVLLFNKKFLQ"
BASE COUNT         2280 a         1335 c         1383 g         2370 t
ORIGIN      
        1 tgttcatgtc gcaggatatt gtagctgttg atacggctgc caccaatttt ttcaatcagg
       61 tgagagacat gccgctggaa agtgtgggac atctggtaaa agctcaggaa ttgaaagttg
      121 gcaccatgaa cctcaatcag ttaaatataa aacggattaa aatgtaaaca gagatgttga
      181 agaaattaag agtcgtcgtt tctctcctgt ttttcatccc gattacttta tattttcttg
      241 attttcgagg tgttttgcca caacagtttc attggctggc aaaaattcag ctggtaccgg
      301 cattgctctc gcttaatctc atcattctga cgataatagc cggaattact ttcatttttg
      361 gacggattta ttgctcatct gtctgtccac tgggcatttt tcaggatatt gtttcatggg
      421 tttccaaaca gatcaataaa aagaaaaaat tcaaattcag tacagcaaaa aacattctcc
      481 gttggtcagt tttgggagtt attgttatta cattttttct tggattctct tttctaaccg
      541 gttttttcga cccttacagc atttatggac gtatggccac ccatttgttt aaaccggtgt
      601 atatggaagg aaacaacttg ctggcaggga ttttcaatgc agcaggtttt cattctctct
      661 acaaagttca gatttacctg ctcagcctcg gcacttttct gattactttg gctatgatgc
      721 tgataattgg ttatttgtca tggaaaaatg gacgtcttta ctgcaatacc atttgtccgg
      781 ttggaaccac attgggattt ctcagcaaat attccctttt taaaatcaga ttcgatcaag
      841 aacagtgcaa ttcgtgcgga ctttgcagca tgaactgcaa atcttcctgt atcgatgcaa
      901 aagaaaaaac aatcgattac tcgcgttgcg taaattgctt caattgtatg gaagcctgca
      961 agcgtaacgg ttttaaattt tccaccaaat ctgctttacg aagaaaaagc acagacaatc
     1021 agaccacaac ggatattgat aataacaaac gacgttttct gagcacactg gctgtttcgt
     1081 cttttgccgt agctggattg cctcaggaag gaatcaaaat aaatggtttt cacaaggtaa
     1141 aaagagaaac tcctgttagt cctcccggat caaaaagtgc cgaaaattta atgagtgcct
     1201 gcacatcgtg tcatctttgc gtcagcaagt gcccatcaca aatcattcgg ccatcacttt
     1261 ttgaatatgg tttgggaggc atcatgcagc caatgctttc tttcgaaaaa ggattttgta
     1321 attatgattg tactgtttgc tccgaaattt gtcccagtgg agctctctca gctttgacca
     1381 aagaagagaa acacaaaaat cagatgggac aggtgcattt tattttggaa aactgcattg
     1441 tatataaaga tgaaacaagt tgtggtgcct gttcggaaca ttgtccgact caagcggtaa
     1501 gcatggttcc ctacaaaaat gaattgacca ttccacatac cgataccggt atttgcgttg
     1561 gttgcggcgc ttgtgaatat gcttgtcccg cctttcccga caaagcaatt tatgtggaag
     1621 gactcaaagt tcatcagcaa gctgccataa aaaaagaaaa atcaaaccac aaaattgaaa
     1681 tagaagattt tggtttttaa gaataaaatt tttgaactat taatttatta tcgacatgac
     1741 acttacaaca attttattag ctatcggaac acccgaaatt ttaatcattg ccttagttat
     1801 tctactgctt tttggaggaa gaaaaattcc agaattgatg cgcggattgg gaaaaggagt
     1861 cagccagttt aaaaaaggaa tgaaagatgt agaagatgaa attaaggaag atgacaacaa
     1921 aaaggaatag ttatcttata tttcaagtta ttcattcttt tcccaaacaa tttttttgag
     1981 taaaaatacc aaaattaata tatggataga actgatttgc aaatgacatc catataacat
     2041 aacgataaca attcggtatg tcgttaaaca ataaggcaaa tgaacaggaa actactttct
     2101 ggtggcattt ggaaaaactt cgctgggtca ttttacgaac attggcagtt gtaggactgt
     2161 taatggtgtt attcttttcc aacaaagatt ttctgtttaa caaaattatt ttagcaccct
     2221 taaattctga tttttatgtc tatcgctggt ttaacgaaat tgcgaagttg ttaaatttca
     2281 gcgatttgac aatagacagt tttcatcttc agcttatcaa tatcaatctg gcagggcaat
     2341 tcatggccca tttgtctatt tcgtttgctt ttgccctcat tgtctcggtt ccatacctgc
     2401 tattcgaaat ttggcgtttt atcttccctg ctttgtatcc gcatgaacga aaaaatatag
     2461 aattaatttt ctttttttct tctttgttgt tttatctggg tgcaatcgtc agttatctga
     2521 ttatttttcc gttgaccatc cgatttttgg gaacctatca ggtttcttcg ctggtaccca
     2581 atcagattgc tctctcttct tacatcagcg ccttgtatat tctcgtcttt tcattgggac
     2641 ttacttttga aatacctgtt ctctcgtact ttttatctcg attgggaata attactcggc
     2701 aaatgttgaa aaaaggacga ggacttgcac ttgtgattat tttaatagct gccgcagtta
     2761 ttactcctac aaccgatcct tttaccatga tggttgtaac tgttccgctt tatttactct
     2821 atgaggtcag tattttggtt tgtaaaaaag acacgggaca ataacgtaat agtttcggag
     2881 ctgataacgt ttcaaatact gtaaagaaat gcatagatac attagtcgaa ttgacaaccg
     2941 atgatgatac tcctttttaa aaactatcaa aacatgtcaa atcgcattaa gtacaaattg
     3001 taaaggcaac aaaatctgca aaagatattg attcgggtta catattaatt ttggcaaggt
     3061 taagttgttg gtgttggatg tattcacaac ttactaacga aaattttggt aatcaatata
     3121 aatgagttct gttataaatt caatagaaga tattttaaaa caacagacca agaaaaaatt
     3181 aatttcttaa caataaatga atttttattg cgaaatttgt aaaaacaaaa agttgcattc
     3241 cttacttgaa tgctctaaca ctactaatta tgaaaaaagt tattcatacc tctgatgcgc
     3301 ccgcagctat tggaccttac agccaagcag tagaaagcag taacattctt tttatttcgg
     3361 gtcaaattcc aatttgccca caagaaggaa aaattatttc aactaccatt gacggacaaa
     3421 ccgagcaggt tttttccaat atcgaagcca ttttgaaatc ggcaggatat tcgttcgggc
     3481 aaatagttaa gactacggtt ttcctgaccg atatgagtca ttttgcagcc atgaacgaaa
     3541 tttacaaaag attttttacg gaagattgtc cggcccgctc aacgattgcc gttaaggaac
     3601 ttcctcaggg agctttggtt gaaatagaaa cgatagccat gaaataaatt tttatcgcag
     3661 gccgcgaaga aattcgccaa tttccattcg ttttttgttg ggggcctgta cttcttttaa
     3721 caccagaaaa ccatctttca acgcaatttt agcaaacttt ttcccatcgg ttttcaaact
     3781 tcccaaaggc aactgatgag aagaaaattc tttctctgcc tgaaaaattt tcaaaaccgt
     3841 tttttcgttt gtatgcggaa aaactaagtt catccatgct ccaggatacg gagagagtcc
     3901 gcgtacaaaa ttatgcgcgc gttcaacatc ccacgaaacg tcgatttcgc aattttccct
     3961 gaaaattttg ggtgccgatc tcaactcaat cgtatttttc aacaattccg actgatcaat
     4021 gggttgcact gcatcagaaa ttatcagatc aacagttttt ttaacggtgg aagccccaag
     4081 catcatcaac ttgtcatgta aagtgccggc attatcggaa ggaagaatag gaattttttc
     4141 ctgaagaata atttttcccg tatcaatttc gtgggtgaga aaaaaagtcg ttaccccggt
     4201 ttccttttcg ccattgataa tcgcccagtt gatgggtgcg gcacctcgat attgcggcaa
     4261 caaggaagca tgaagattaa aagttcccaa tggaggcatg ttccacacta tctccggcaa
     4321 catacggaaa gcaacaacta tttgcaaatc tgctttcaga gattgtagat tttccagaaa
     4381 aatttcatct ttgagttttt ccggctgaag aagtaacaga ttattttcaa gtgcatactg
     4441 ttttacaggc gaaaactgaa ccttgtatcc acgtcctgca ggcttgtcgg gcatagtaac
     4501 tactcctacg acattgtatc cgccttcgac aagaattttt aaactttcaa ccgcaaactc
     4561 gggtgtcccc ataaaaacaa tacgaagctc ttcctttgat tttctcatgt cattttttta
     4621 aaatattgca gcaattttta attcggtctc aacacaaaaa taataaaaaa attttcatgc
     4681 tacggtcatc aaaaaataaa agcattaaca acgtggagaa attttacagt ttatcttcaa
     4741 aaaatctaat tttttcactc ttttcgaaaa aaaaaggttt taatagggaa cccaaatttt
     4801 taagcaaaaa ttatcattaa ctcaattttt ttttgtaact ttatcctgta aatctcgatt
     4861 ttaatgaaga aattatattt attatccttc gcatttattt tatgtttttc tttaagttct
     4921 accgctcaaa aagttgggtt agtattgagt ggtggcggag ccaaaggcgt tgcccatatt
     4981 ggagttatta aagcgttgga agaaaacggt attccaatcg attacatagc aggaacttct
     5041 atgggggcga tagttggcag catgtatgca atgggactta caccagagga aatgttgaat
     5101 attatcaagt ccgacgattt tagattctgg cttaccggac agaatgaacc cgaatatgtt
     5161 ttctattttc gtaatggtaa tgtaaagcca acatttatgg aaatcagttt tcgtttcaat
     5221 ccacatgata cagtgagggt taaaagcaat gtcctcccta cgcatattgt tcctacccat
     5281 caactcaatt atgcttttat ggctttattt tcacaggcaa cagccgcttc cgaacagaat
     5341 ttcgatcgac tttttgtccc gtttcgttgt gtagcttccg acattgagga aaaagttccg
     5401 gtagttttca gcaagggctc attgggcgat gcagtaaggg cttccatgac ttttccattg
     5461 gtttttaaac ccatcgtaat agacaacaaa ttgctttttg atggaggtat ttacaataat
     5521 ttccctgtag atgtgatgcg gaatgacttt aatcccgata ttatgattgg gagcgttgtg
     5581 gcaaaaaatc cgcaaaaacc cactgaagac aatcttcttt tacagattga aaatatggtg
     5641 atgtctcaaa ccgattatgt tattccaaaa gaagaaggct ttatgcttcg tttcgattta
     5701 aagaatttta atatgtttga ttttacaaaa gctgatcagc tttatcaaat gggttacgat
     5761 tccacacttc agaaaattga ggagataaaa tcggccattt caagaagaat ttcagaagaa
     5821 gaactaaata aaaaccgaca ggactttcgg gataaatttc ctgaacttaa attccaaaat
     5881 gtttatatag aaggaataga ttctttgcaa aaactttatg tggaacgcga atttcatact
     5941 gggaatgaag ttttcgattt gaaaagtttc aaaaaaagtt atttcaatct cctttccgat
     6001 aatcagattt cggaaatcac gcctgcagcg gtgtacaata aaacttccgg tttatttgat
     6061 ttacatttga aagtcaaaac cgaagatcat ttgaaggtta tgatgggagc cgacatttca
     6121 tctatggcaa gcaatcaggc atatatcgga ctttcatatc aaaatttgaa ggaatatgcg
     6181 caaacggctt atgtggatgc tcagtttggt agaatgtaca atgcgctggg agttggtgtt
     6241 agaatggatg cggcagccaa aaccgatttt tatgtgaagt ttggctttgt aacgcataag
     6301 tttgattatt ttgatggaat cagcttgttt tataacgata atcgcgtgtc gtatttcaat
     6361 cagcaggaaa catacggtaa attgagcatt ggatttcctc tgacaaaaaa aggtagaata
     6421 gaatttgggc tgggaggagg ttatttagcc gatcattatt ttcagaacaa aagcttgatt
     6481 acttcgggag taaaaaacga caaaagcagt tattcattgg gaagcgtttt tgttgatgcc
     6541 gaaagtaaca cgctaaatcg tccaaattat cctactggcg gatcttatta tgctgcttcg
     6601 ttacgtttga ccggtggtaa tcacaatgaa tatcttaatt atcctgttgt gagggaactc
     6661 gaagggcaag agttgtggct gcagtttctt ggaaaatatg atcgatatat caacctttcg
     6721 aaaaatgtta cattgggaac ttatggggaa ctggccattt ctaaccgaaa attgctaccc
     6781 aattatacgg ctactatgat tcagtcgcct gcattccgtc cgacgttgca cagtactacc
     6841 attttcaacg agggttacaa cgccaatcaa tacgcagcat tcggactgaa acccatttac
     6901 agtttcaaca atcaattgca ttttaggacc gaagcctatt gttttgttcc atataaaacc
     6961 atttatcgtc ttcctgatga atcggcagca tattccaaac ctttttccga cgtggaattt
     7021 atttcggaag ctgctatggt tttaagcttc aaaaaagcaa cactttctgc attcgtgaat
     7081 tattacagcg ctggttcgaa tcattggaat gtaggtttta atattggcgt tcttcttttt
     7141 aacaaaaaat ttctgcaata aaatctgagt gtaatgattt gtaggcaaag agcaaaaaat
     7201 taatttcatc tttccgaaaa aatgatgaaa tatcttttta gaagtagatt aaaataatgg
     7261 aaatacctat tccaataggg aagagaaagt aaatttattt ttcgggacaa atataaaaaa
     7321 gaggctgtct caaaagtaga gacagtctct tttttattgg atattgcg
//
LOCUS       DAOTAX010000078         7264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8716_length_7264_cov_29.046747, whole genome shotgun sequence.
ACCESSION   DAOTAX010000078 DAOTAX010000000
VERSION     DAOTAX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8716_length_7264_cov_29.046747"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..522)
                     /locus_tag="PLM04_07500"
     CDS             complement(196..522)
                     /locus_tag="PLM04_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02954.1"
                     /translation="MDISEQYKDLTEKIEKNLSLTAMLIRELVQIFRDKENSITLKTE
                     LTITNVHNSGVISGIMCTVQNNNENVIICPLSDLIFSTNCLLYREISDYQKKREKRIK
                     KLNQMG"
     gene            complement(534..848)
                     /locus_tag="PLM04_07505"
     CDS             complement(534..848)
                     /locus_tag="PLM04_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02955.1"
                     /translation="MDTNDLSRETHTAVIDTAERFHHDLTLQFGVLASDCKTDNEYLD
                     QSESMIKEWLTDWDLEEVIMDIFFDDAPRKQDFKKVLDKILTNIEKVRRIPIEQRKFD
                     LW"
     gene            complement(1018..1575)
                     /locus_tag="PLM04_07510"
     CDS             complement(1018..1575)
                     /locus_tag="PLM04_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02956.1"
                     /translation="MKKILFIVLTTLTLTTYGQDKYNYVHFNKLTEVVGTEFVIASIE
                     NWGKMLETKTRYLLFVNTKTGETNQVDFPNDAGIGKIEQIIIDSLNLNVIVVSARTVD
                     LDGKNGIDWNDPTQIIILSTDGKTKTQLTDNKFFVRTWTVNNLTGTLVVTGHYDTNNN
                     NKYDKTDKNEIHIYDLKTLKLINKI"
     gene            complement(2256..3026)
                     /locus_tag="PLM04_07515"
     CDS             complement(2256..3026)
                     /locus_tag="PLM04_07515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HON02957.1"
                     /translation="MKNEKQTIHEFDLNIIYDFFSKTERQGPGSPEETLKALSFIDGL
                     TEKSKIADIGCGTGGQTMVLGENTSCEIIGVDVWPDFINQFNQNAQIKNCQDRVKGIV
                     GNMENLPFQEEELDLIWSEGAIYNIGFKRGLNEWRKFLKQGGYIAVTENTWFSEERPA
                     EIQDFWQKAYPEIDTIPNKVAQMQKAGYLPIATYILPETIWTDYYSWQALRLEPFLKK
                     HNGNKIVEEFVASQRYEAELYYKYKAYYGYMFYIGKKM"
     gene            complement(3477..4925)
                     /locus_tag="PLM04_07520"
     CDS             complement(3477..4925)
                     /locus_tag="PLM04_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HON02958.1"
                     /translation="MEKTTILSALLIVYSFCFGQKDETLLENYPLKINHYNLDLSFDF
                     QEGYLNGICDVTIENKSDKPVNEIPLLLYRLMKVASIQNQQGEELEFVQGVVSFSDFE
                     KLQANSVTINENIKPQSATTVRIKYEGYLLGYQETGMRYIKDKISPDFTIIRNDAYSY
                     PILAKPSIAFLRKNIASNNFSYRINITVPDSLMVANGGLLLLKTSNNKNTTYKYESKK
                     PNWRIDIAIAPYHFETTDRLSIFYFNDDEEIAKTLLNKGNEVIRLYTKWWGNLKNQNS
                     ITLIETEKHSGGQADETTILLPQESFSSDSYAQLYHELSHLWNVTISEKEGLSPRWEE
                     GLASFCQYYVDEYFNPEKTGLLDKVVNGTIKRLKRSFDANPEMYNIPMCEFGNKGETG
                     YSYNQGMVMFAVLHKWLGKEKFDLAVRSFYERYYGMGATTKNFTELWVKEANVKGLKE
                     FFNDWVYTTNYTSFIKKENTLDEIVKYYNDKK"
     gene            complement(5254..5625)
                     /locus_tag="PLM04_07525"
     CDS             complement(5254..5625)
                     /locus_tag="PLM04_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02959.1"
                     /translation="MNVKNPDLVHIRGIYSDWFKENFSQLWIGFGGSPQTTPKEDAYY
                     VRLYMEAPTSAIMHIGIVDYIERYENGADFYSKAIIKLKNPVNPGHAIIKHENWTLSQ
                     LGLNQNEMDNIRNQLFLIYFD"
     gene            complement(5627..5860)
                     /locus_tag="PLM04_07530"
     CDS             complement(5627..5860)
                     /locus_tag="PLM04_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02960.1"
                     /translation="MSDFRDEYNKVKPKVILIFKRIYVLAFNIYAWYWLYRQIFIKHS
                     TCFEDYLLWFFATFGMYVFILEHQEIFTKPKKK"
     gene            complement(6022..7143)
                     /locus_tag="PLM04_07535"
     CDS             complement(6022..7143)
                     /locus_tag="PLM04_07535"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HON02961.1"
                     /translation="MRKCIIFLILSIFSIFVFGQETTQVNQAEINSFPIPKVDKRVEL
                     LSIVFRLAGNREYNDENYQVYINDIHKHFDKYQGHELIAFATQLRNKNGVSYDAVMKM
                     AIYIEQPPSLNPIVDFTSDIPEGRWGQENATRFIKLLQQFYVDAKCDEFFKAHEELYK
                     NIPKRFKKVYDALDINWFNQYYGVQPKGSLNIILGLGNGGGNYGPKVILPDGQEDSYA
                     IIGTWPLDSLGEPEYNVNDNLPTLIHEFNHSYVNHLTNINMSKFEASGVKLFDIVRES
                     MEKQSYGNWQIMMNEALVRASVIRYYLRHNSNSAVAKTEMIRQFNKGFFWIKELVDCL
                     GEYENKRDKYPTLESYMPVLIDFYNRIAKNTESMFEIKN"
BASE COUNT         2119 a         1401 c         1145 g         2599 t
ORIGIN      
        1 tattttaaat taactatata catagactag ttcgttatca tcgccttgtt gccaaggttc
       61 cggtggtttg ggctgctggg gagttaagag ccaacggcgt catcccacgt taaaaccatg
      121 ctaaattact aaaaactttc gtatctgcaa atctacctca gtaggctaaa ccacctgttg
      181 taggctgggc gtttttcatc ccatttggtt taatttctta attctttttt ctcttttttt
      241 ctgataatca ctaatctctc ggtatagtag acaattagtt gaaaaaatca aatctgatag
      301 agggcaaatt ataacatttt cattattgtt ttgaacagta cacattatgc ctgatataac
      361 accactatta tgtacattcg taattgtcaa ttctgtcttt agtgttattg aattctcttt
      421 gtctcgaaaa atctgcacca gttcccttat tagcatagca gtcaatgaaa gatttttctc
      481 aattttttct gtcaaatcct tatattgttc tgaaatatcc attatttatc agtttaccaa
      541 aggtcaaatt tacgttgttc aattggtatc cttctcactt tttctatgtt tgtcaatatt
      601 ttatcaagta cttttttaaa atcctgcttt cttggtgcat cgtcaaagaa aatatccatt
      661 attacttctt ctaaatccca atctgttaac cattctttaa tcattgattc tgactggtca
      721 agatattcat tgtcagtctt gcaatcactt gccaatacgc caaattgtaa agtcaaatca
      781 tgatgaaatc tctctgctgt atcaataact gcagtatgtg tctctcgtga taaatcatta
      841 gtatccatat gctgtgtcgt ttttctcgcc ttgcctacaa cggtttgcaa tatgaccagt
      901 ggcggattgc gggtgacatc cctgtcaacc gttacaaaac tttgcgcggg gcagacgctt
      961 tcatgctacc actgcgtccg acattggtta tatttagtgt tatgcggtcg gctttttcta
     1021 tatcttgtta atcagcttta gtgttttcag gtcgtaaatg tgtatttcgt ttttatcagt
     1081 cttgtcgtat ttgttattat tgtttgtgtc atagtgtcct gtaactacta aagttccagt
     1141 caaattgtta actgtccatg ttcttacaaa aaacttgttg tctgtcagtt gagtttttgt
     1201 tttgccgtct gtggataaaa taatgatttg cgtcgggtcg ttccagtcaa ttccgttttt
     1261 tccgtccagg tcaacagttc tggcagaaac aacaattacg ttaaggttta aactgtcaat
     1321 tattatttgt tcaatttttc ctattcctgc gtcatttggg aagtcgactt gattagtctc
     1381 ccctgtcttt gtattaacaa ataacagata tctggttttt gtttcaagca ttttccccca
     1441 gttctcaatt gaagcaatta caaattccgt cccgactact tctgtcagtt tattgaagtg
     1501 aacgtaatta tacttgtcct gtccgtaagt ggtcaaggtc aaagttgtca gaacgataaa
     1561 tagtattttc ttcattatca gtctattttt ttgttgttgc tgtcgtctta agctgccgca
     1621 taactcgttt atatatgtga taaaactaca taaagtcatc catttggggt gtatatatgt
     1681 aactgatatc acatttattt ttttcaaaga taaatcgagt ctcatccaaa aaccctgttt
     1741 taaaatttta caaaaataat atttttttgg acataaacaa attttttttc aaatttgtat
     1801 tacaaatttc aaaatttgga caaaatttat ccaaagacaa agtacatttg ctcatattat
     1861 atgctttatc aattcttggg agctactttt gtttggataa tggtatccta attgaaaaat
     1921 ggtagattca acattatttc gtttttgtaa tatttctatg ggagttgttc aaatctgtgg
     1981 agtggtcgtt acacttgcca ctaacgttca gaggtatgaa acgtagcgga ttgcgtggct
     2041 gcaaacctat ccctcgttac tgaccttgcc agcgggaacg gaacttgcaa attacactga
     2101 aaccactatg ttttatgact gcttattatc agctggcatt tgtcccctgt cattgaaatt
     2161 agaaaaaaac ctttcgtgtg ttaaagctcc cacacgaaag gaattttcaa attcacattt
     2221 taaacaaacc aggaatttac agctggcttc attctctaca ttttctttcc aatataaaac
     2281 atatagccgt aatatgcttt gtacttgtaa tataactcag cttcatatcg ctgggatgct
     2341 acaaattctt ctactatttt attgccgtta tgctttttta gaaagggttc taatctcaag
     2401 gcttgccaac tatagtaatc tgtccaaata gtttcgggca agatataagt ggcaatcgga
     2461 agatagcctg ctttttgcat ttgagcaacc ttattgggaa ttgtatctat ttcgggatag
     2521 gctttttgcc aaaaatcctg aatttcggca ggccgctctt cgctaaacca ggtattttct
     2581 gtaaccgcga tatatccacc ctgtttcaag aatttccgcc attcgttcag tccacgttta
     2641 aatccaatgt tataaatcgc accttccgac caaatcaagt ctaattcttc ttcttgaaag
     2701 ggaagatttt ccatattgcc gacaatgcct ttcactctat cttgacaatt tttgatttgg
     2761 gcattctggt tgaattgatt tataaaatcg ggccagacat caacaccgat aatttcacac
     2821 gatgtgtttt ctcctaatac catagtttga ccacctgttc cacaaccgat atcggcaatt
     2881 ttggactttt cggtaagacc gtctataaaa ctcaatgctt taagagtttc ttcgggactt
     2941 ccgggacctt gacgttctgt ttttgaaaaa aaatcataaa taatattcaa gtcaaattcg
     3001 tgaattgtct gtttttcgtt tttcattttc atttattttg ttgcatacac aaaaagcatc
     3061 ataatgcttt catcattgta cgcaaattaa accctaaatt aattttatag aaaactgctc
     3121 tcgaaaagga ttatacgaga acaaacaaag acacaatcta tacaaaaagt acagattgtt
     3181 tacagaacaa tatccgaatt aaaaataaac atccaattgt tttaatgtgc gcaaaggtat
     3241 aaaaaataat gaaacttgaa acttttttca aatctatttt tcggaagtcg ttatcttcga
     3301 aaccgtttta tgcttgctgc taacgttttc gggctttgcg ttcgggcggg tttagcttcg
     3361 ctgactttca gttcggagca caaaactgtc tgccagcata acacttgaat agaagcacaa
     3421 agctccaagt ttgcacgtca ccccgcttga tgcaaaaccc gtgttagcgg ctgtttttat
     3481 tttttatcgt tatagtactt cacgatttca tcaagcgtat tctctttttt aataaacgac
     3541 gtataatttg tagtataaac ccaatcattg aagaactcct ttaatccttt tacatttgct
     3601 tcttttaccc ataattccgt gaaattttta gtcgttgctc ccataccgta atatctttca
     3661 taaaaggaac gaacagccaa atcgaatttc tcctttccga gccacttgtg tagaaccgca
     3721 aacattacca ttccctgatt ataagaatag cctgtttctc ccttattccc aaactcacac
     3781 attggaatat tatacatctc aggattagcg tcgaagctcc tttttagtct ttttatcgtt
     3841 ccattcacaa ctttgtccaa cagtcctgtt ttttcaggat taaaatattc atccacatag
     3901 tattggcaaa aagaagccag cccctcttcc catcttggcg acaatccttc tttttcgctt
     3961 atggttacat tccaaagatg tgaaagttcg tgatacagtt gagcataact atccgaagaa
     4021 aatgactctt gaggcaaaag aattgttgtt tcgtcagctt gaccaccgct gtgcttttca
     4081 gtttcaatta gagtaataga gttttggttt ttcaaatttc cccaccattt cgtatagagt
     4141 cgtataacct catttccctt gttcaataat gttttagcta tttcttcgtc atcgttaaag
     4201 taaaagatgc ttaatctatc ggtagtttca aaatggtacg gggcaatggc tatatcaatt
     4261 ctccaattag gttttttact ttcatattta tatgtggtgt ttttattatt tgaagttttt
     4321 aacaagagga gtccgccatt agcaaccatt aaagaatcag gaacagttat atttatccta
     4381 tatgaaaagt tgttagatgc gatatttttt cttaaaaacg caatactcgg ttttgcaagt
     4441 ataggatagg aataggcatc atttctaatt atcgtgaaat ccggcgatat tttgtctttg
     4501 atataccgca ttcctgtttc ctgatacccc aataaatagc cttcatactt tattctaacc
     4561 gtagtagcac tttgcggctt tatgttttca tttattgtaa cggagtttgc ttgcagcttt
     4621 tcaaaatcag aaaacgaaac aacaccttga acaaattcaa gctcttctcc ctgttgattt
     4681 tggatagagg caactttcat cagtctataa agcaaaagcg gaatctcatt taccggttta
     4741 tctgatttat tttcgatggt aacatcgcag attccattca aatacccttc ttgaaaatcg
     4801 aatgataagt ctaaattgta atgattaatt ttcagtggat aattttcgag taaagtttcg
     4861 tctttctgcc caaaacagaa actgtaaact attagcaatg ctgaaaggat agttgttttt
     4921 tccatttttc tttgtgttta agtgctgata ttatggatgt tgctatttaa aattctgact
     4981 aacgtcaaat tctgttactg ataaccagtt gtttataatt tttgttaaat ctaaatttcc
     5041 atcacatttt tagtttctac atttgtcgtt aacgcgagaa attaatcaga tcgtccgagg
     5101 tggtaaatta ctgctaacgg tggcggtatg aaacgtgccg gattgcggga ctgcgaccgt
     5161 atcccccgtg acaaacctac gggggggacg aaacgctcga atacccactg aacccggcat
     5221 gttttatgaa cgcgtgttgt gtgccgtttt tttttaatca aaatatatca aaaacaactg
     5281 atttcgaatg ttatccattt cgttttggtt taatcctaat tgagataaag tccaattttc
     5341 atgttttata atcgcatgtc caggatttac tggatttttt aattttataa tcgcctttga
     5401 ataaaaatca gcaccatttt cataacgctc aatgtagtca actattccga tatgcattat
     5461 tgccgatgta ggtgcttcca tataaagtct tacataataa gcatcctcct tgggtgtcgt
     5521 ttgtggcgaa cctccaaatc caatccaaag ttgactaaaa ttttctttaa accaatcaga
     5581 gtatattcct ctaatgtgaa ctaaatcagg attctttaca ttcatcttat ttcttttttg
     5641 gtttggtaaa aatctcttgg tgttctaata tgaatacata catgccaaat gtcgcaaaga
     5701 accataataa ataatcctca aagcaagtgg aatgttttat aaaaatctgt cgataaagcc
     5761 aataccaagc ataaatatta aaagctaata cgtaaatcct tttaaaaatt agaatcactt
     5821 ttggttttac tttattatat tcatctctga aatcactcat ggcagtctat tttcaaaata
     5881 acacacaacg tcggtggtgt agccagtaag ggattgcgga gcgacgttcc tatcaaccga
     5941 gacaaaagtt tgagcgggct acaacgctta aatacccact gaaaccctta tggcatatac
     6001 actttgttgg gcgttcgttt tttagttttt aatttcaaac attgactcgg tattcttggc
     6061 aattctatta taaaaatcaa ttaaaactgg catataactt tcgagtgttg gatatttatc
     6121 tcgtttattt tcatattccc ctaagcaatc aacaagttct tttatccaaa aaaatccttt
     6181 attaaattgc cttatcattt ctgttttggc tacagcacta ttggaattat gccttaaata
     6241 gtagcgtata acagaagctc tgactaaagc ctcattcatc attatctgcc aattaccata
     6301 cgattgtttt tccatacttt ctctaacaat atcaaaaagt tttaccccag aggcttcaaa
     6361 ttttgacata tttatattcg tcagatggtt aacataagaa tggttaaact catgtattag
     6421 tgtcggcaga ttatcattta cattatattc tggttcacct aaactgtcta gcggccaagt
     6481 accgattatc gcataactat cttcttgccc atctggaagt attactttag gaccatagtt
     6541 gcctcctcca tttcctagac ccaaaataat atttaaactg cctttaggct gaacgccata
     6601 atattgatta aaccagttaa tatccaaagc atcataaacc tttttaaacc ttttaggtat
     6661 attcttgtaa agctcctcat gtgctttgaa gaactcatcg catttggcat caacataaaa
     6721 ttgttgaagc agttttataa atctagttgc gttttcctga ccccatctgc cttcaggaat
     6781 atctgatgta aaatcgacaa ttggattcaa actcggtggt tgttcgatgt aaattgccat
     6841 tttcataaca gcatcatatg agactccatt tttgtttctt aattgagtgg caaaagctat
     6901 caattcgtgg ccctggtatt tatcaaaatg cttgtgaata tcatttatgt aaacttgata
     6961 attctcatca ttatactccc tattacccgc taatctgaac acaatactta ataactctac
     7021 acgtttatca actttcggaa tgggaaaact attaatctct gcctgattga cttgagttgt
     7081 ttcttgtcca aaaacaaata ttgaaaatat tgataaaatc aagaatataa tacactttct
     7141 cataatacca aagtttaatc ttttattgtc tgaatataag ttgctcgctg ttcaatcaaa
     7201 atgacgccca acgtgccgcc gctaaacaca gtaaacgaca atcgatatat gaaaaccgta
     7261 acag
//
LOCUS       DAOTAX010000079         7132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_8912_length_7132_cov_30.284584, whole genome shotgun sequence.
ACCESSION   DAOTAX010000079 DAOTAX010000000
VERSION     DAOTAX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8912_length_7132_cov_30.284584"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..2573
                     /locus_tag="PLM04_07540"
     CDS             75..2573
                     /locus_tag="PLM04_07540"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HON02962.1"
                     /translation="MNKSLFQKLLPHFVALILFFCLSFWYFSPVLEGKQLMGHDTQSW
                     LYSAKETIDYNQTHDDKTLWTNSMFGGMPTYQISMTQPNNIIKYVDQLIKSLPSPVFF
                     LVLYLLCFYILLLAFGINPWLAIIGSFAFSFASYNFIILSVGHNSKAITIAYIAPLIG
                     SIYMAFRSKRMLGSLLTALFLSLAIYANHIQILYYTLLMVLVFGLVELIFSIKEKKAS
                     SYFKTLGLLIIAALLAAGMNATSLLTTYEYNQYTMRGKSTGLTLDEQNAQHGLNKDYI
                     TQWSYGIDETLTLLIPNFKGGASAGTLSSESKTAQRLSSYGVPNVERMMKEMQLPLYW
                     GNQPGTAGPVYVGAIVCFLFVLGIFLVDKRTKWWLLPLIVLSIMLSWGKNFMPLTDFF
                     IRYIPFYNNFRTVSMILVIASFGMALMAILALKEVFNPATDKAKLLRPVSISAAIVGG
                     ICLIFAVVPSLAGNFVSPADGQFTGDYAFLKETLPLDREALLRSDALRSLTFVILCAG
                     LIWLYAKNLVKKNLAYVLLGCLMLGDLVPVAKRYLNDDNFTRKRSFEKFIQPSVADKI
                     ILQDKSQFRVLDATVDIFNDASPSYFHKNIGGYHAAKLRRYQELINVHLTKELGQLFG
                     AFGRAKTIEEIVQVMDSLGVLNMLNMKYVIYNKEAPPLVNPYANGNAWLVTKVRIAEN
                     ADEEMRLLGEINTKTEVVVDKSFSALLPAAIIPDSSAHIQLVSYEPNHLIYNFSSKTD
                     QLAIFSEIYYDKGWNAYINGKKVPYIRANYLLRAMPLKAGSYQIEFKFEPDSYRIGNS
                     IALVSSILFVLWLVGYIYLRWKKPSTAKKIDQWE"
     gene            2636..3517
                     /locus_tag="PLM04_07545"
     CDS             2636..3517
                     /locus_tag="PLM04_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HON02963.1"
                     /translation="MGKNNNLHGKRSIKNIHFISTFSMSLVLFLVGLVILLLLTARNL
                     GINVRENINLSIILDDEIKNTEIQRIQKYLLSSPYAKSVRFISKEEALQEHIESLGED
                     PQKFLGYNPLLASFEVKLKAEYANNDSVEMIESKLKRFDHINRIAYQKDVVDLVNRNI
                     QKLSYILLGIALVLLLVSIALINNTIRLLIYSNRFLINTMKLVGATWWFIRKPYIKQG
                     MINGLVASILSLLYLTATIYYVKYEFGISGIVIPFGTALMVSSIILFLGVTLTAISSY
                     LAVGRYLRMGTNNMYFV"
     gene            3554..3979
                     /locus_tag="PLM04_07550"
     CDS             3554..3979
                     /locus_tag="PLM04_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HON02964.1"
                     /translation="MKELTLITPTFLFSAVSLILLAYTNRFLSYAQLVRTLKDRYMED
                     HSAVTRAQILNLRKRLSLTRLMQLFGVASLLLCVATMFLIYVGFQTVSAYVFGIALVF
                     LIVSLSFSVWEIQISTGSLKIYLEEMENEEIREEEKLKT"
     gene            3984..5891
                     /locus_tag="PLM04_07555"
     CDS             3984..5891
                     /locus_tag="PLM04_07555"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HON02965.1"
                     /translation="METNFGFVRIAAAVPELKVSDILFNTEKIIELIHVAEENRAQVV
                     CFPELCITGYTCADLFFQQQLQEEVLKALSEIQMATLSVSCAVIVGAPLRFRNRLFNT
                     AVVLQKGRIIGIVPKTFLPNHGEFYEKRWFVSGKNLRGQTIVCNEQEVPFGVDLIFFS
                     PSFSFGIEICEDLWAPLPPSTELCMAGAEIIFNPSASNELIGKHEYRLQLIEQQSARC
                     HAGYVYSSSGMYESTTDLVFSGSAVIAENGKILETSKRFSLDSQLIFNEIDVEILRYE
                     RMHNSNFGEDALQQTYRKIEMQGQPLEQKEIKRTVSPYPFVPPVSKREESCSEIFSIQ
                     TSGLAKRWLHTQAKSLIVGISGGLDSTLALLVCVKTADKLGFDRKTVIGVTMPGFGTT
                     NRTYGNALNLMKSLEITGLEISIKEACLQHFKDIGHDPDVHDVIFENTQARERTQILM
                     DLANKYNGLVIGTGDLSELALGWATYNGDHMSMYAVNSGIPKTLVRYLVEYVAQSMEE
                     KTKTILEDILKTPVSPELLPADEYGEIHQKTEDLVGPYELHDFFLYYFVRFGFSPKKI
                     LFLAQKAFGDKYSEAILRKWLKVFLRRFYSQQFKRSCMPDGPKVGSINLSPRGDWRMP
                     SDAGFHWDDII"
     gene            5933..7000
                     /locus_tag="PLM04_07560"
     CDS             5933..7000
                     /locus_tag="PLM04_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HON02966.1"
                     /translation="MLLFFERIILGNRLSDWGISLLIIVGAFLLNKGISLLNKNVIQK
                     ITAKSKNRLDDLLVKTLEAPILLGIALIAVWVAARRLDLGTQVDQGFYRAYQILTVLN
                     ITWFLVKLTGALINEYLLPHSKEEEDNKRLDESMVRILRRISVGIIWAIGIVMALNNA
                     GVNVGALLASLGIGGLAVALAAQDTLKNFLGGLTIFIDRPFRIGDRIVTGAYDGFVED
                     VGIRSTRIRNFDRRIISIPNYKIVDAAIENISIEPMRRVVLKIGLTYNTPPEKMKEAI
                     TILKNIPQKVDEVFPDEIYAYFTDFSDSALTITFIYFIKKEANVMETPSKVNMNILED
                     FNAAGLNFAFPTRTVYIENQN"
BASE COUNT         2012 a         1334 c         1519 g         2267 t
ORIGIN      
        1 agcttttgaa aaaaaataat gtatttttgt actcaaattt cctcatttag ctgaaaatat
       61 aaaccatcat tcttatgaac aaaagtttat ttcaaaaatt gctgcctcat tttgtagcac
      121 ttattttgtt tttttgtttg tcgttttggt atttttctcc cgttttggaa ggcaaacaac
      181 tgatgggaca cgatacccaa agctggttgt actcagccaa agagaccatt gattataacc
      241 agacacatga tgataaaaca ttatggacta actccatgtt tggtgggatg ccgacttatc
      301 aaatttcaat gacgcaaccc aacaatatta ttaaatacgt cgaccaactt atcaaatcct
      361 tgccttcacc ggtatttttt ctggttcttt atcttttgtg tttttatatt ttgcttttag
      421 cttttggtat taatccctgg cttgccatta ttggttcatt tgccttttcc tttgcctctt
      481 acaattttat tattctgtct gtcggacata attccaaagc cattacgata gcttacattg
      541 caccgcttat cgggagcatt tacatggctt tccgttccaa gagaatgttg ggaagcctgc
      601 tgacggcact tttcctgagt ttagccattt atgccaacca tatccaaatt ctttattata
      661 ctttgcttat ggttttggtt tttggattgg tggaactgat cttcagtata aaggaaaaga
      721 aggcaagttc ttattttaaa acgttgggat tgttgattat tgctgctttg ctggcagcgg
      781 gaatgaatgc cacgagttta cttactacct acgaatacaa tcagtacacc atgcgcggaa
      841 aatcgacagg acttaccctt gatgaacaaa atgcgcagca cggactgaac aaggattata
      901 ttacacaatg gagctacgga attgatgaaa cgctgacatt gcttatcccg aattttaaag
      961 ggggtgcaag tgccggcaca ctttcatccg aaagcaaaac agcccagcga ctgtcaagtt
     1021 atggggttcc caatgtggaa aggatgatga aggagatgca attacctctg tattggggta
     1081 atcagccggg aactgccgga ccggtgtatg ttggagccat cgtctgtttt ctgtttgtgt
     1141 tgggaatttt tttggtggac aagcgtacaa aatggtggct gttgccatta attgttcttt
     1201 ctattatgct ttcgtggggg aaaaatttca tgccccttac cgactttttc atccgatata
     1261 ttccctttta caataatttc cgcacggttt ccatgatttt ggtcattgcc agttttggta
     1321 tggcattaat ggcaatactc gccttaaagg aagtattcaa tcccgcaaca gacaaggcaa
     1381 aacttttgcg tcctgtttcc atcagcgccg caatagttgg tggtatttgt ctgatttttg
     1441 ctgttgttcc ttctttggcc gggaattttg tttcgcctgc cgatgggcaa tttacgggtg
     1501 attatgcttt tttgaaagaa acacttcccc tcgacagaga agctttactc cgcagcgatg
     1561 ctttgcgttc attgactttt gttattcttt gtgccggctt gatttggctg tatgccaaaa
     1621 atctggttaa aaagaatctt gcctatgttt tgttgggatg cttgatgctg ggagacctgg
     1681 tgccggttgc caaacgatat ttgaacgatg ataattttac gcgaaaaaga agctttgaaa
     1741 aatttattca gccatcggta gccgataaaa ttattcttca ggacaagtcg caattcaggg
     1801 tactcgatgc cacagtggat attttcaacg atgcttcacc ttcatatttt cacaaaaata
     1861 taggcggata ccatgcggca aaattgcgtc gttatcagga acttatcaac gttcatctca
     1921 ctaaggagct cggacaattg tttggagcgt ttggaagagc caaaacaatt gaagagattg
     1981 tgcaggtaat ggattctttg ggcgttttaa atatgctgaa catgaaatat gtaatttaca
     2041 acaaggaagc gcctccattg gtcaatcctt atgccaacgg caatgcatgg ttggtgacaa
     2101 aagttagaat agcggaaaat gcagatgaag aaatgcgttt gttgggtgaa atcaatacca
     2161 aaacagaagt ggtggtggac aaatcttttt ctgcgctatt gcctgctgcc attattcccg
     2221 attcgtctgc tcacattcaa ctggtaagct acgaacccaa tcacctgatt tacaatttca
     2281 gttccaaaac cgatcagctc gctatttttt cggaaatata ttacgataag ggatggaatg
     2341 cctatatcaa cggcaagaaa gttccctata ttcgtgccaa ctacttgctt agagcaatgc
     2401 cactgaaagc gggttcttat caaatagaat tcaaatttga acccgattct tacagaattg
     2461 ggaacagtat agcccttgtt tcttccatat tgtttgtgct ttggttggtg ggttatattt
     2521 atttaagatg gaaaaaacct tctacggcaa agaaaatcga ccaatgggaa taatttctgg
     2581 aataaggatt tgtaaagggt tcaaagagat aattatttga aaaagaatat cggaaatggg
     2641 taaaaacaat aatcttcacg gtaaacgaag tataaaaaac atacatttta tttctacctt
     2701 cagcatgtcg ttggtgctgt ttttggttgg actggtgatt ttattgcttt tgacggcaag
     2761 aaatctgggc ataaacgtaa gagagaacat taatctttcc attattttgg acgatgaaat
     2821 taaaaacaca gagattcaga gaattcaaaa atatcttctt tcttctcctt atgccaaatc
     2881 ggtgaggttt atctccaaag aggaagcttt gcaggagcat atcgaaagtt tgggcgaaga
     2941 tccgcaaaag tttttgggat acaacccttt gctggcttct ttcgaggtga aactgaaagc
     3001 cgaatatgcc aataacgaca gcgttgaaat gattgaatct aaactaaaaa gatttgacca
     3061 tatcaatcgc atagcctatc agaaagatgt agtagatttg gtaaatcgga atattcagaa
     3121 attgagttac attctgctcg gaattgcttt ggtattgttg cttgtttcta tagcattgat
     3181 aaacaacacc attcgactgc tgatttattc caatcgtttt cttatcaaca ccatgaaatt
     3241 ggtgggtgct acctggtggt ttatccgcaa accttatatt aagcagggaa tgatcaatgg
     3301 acttgtggct tctatccttt ctctgcttta tttgactgct acgatttatt atgttaagta
     3361 tgaatttggt atttcgggaa tagtaattcc ctttggtaca gctttgatgg tatcctctat
     3421 tattttgttt ttgggtgtta cgcttactgc tatttcatcc tatttggcgg taggcagata
     3481 tttgcgtatg ggcaccaaca atatgtattt tgtataattt ttttcggttt tttaatttgt
     3541 aactgtcttt accatgaaag aactcacctt aattaccccg acttttcttt tttcggcagt
     3601 ttcgcttatt ttgctggctt ataccaatcg ttttctgtcg tatgcccagt tggtgcgaac
     3661 acttaaagat cgttacatgg aagatcattc ggcagttacg cgggctcaga ttttaaatct
     3721 ccgtaaaaga ctttcgctta cgcgactgat gcaattgttt ggcgtggcta gtttattgct
     3781 ctgtgtagct accatgtttc ttatttacgt cggttttcaa accgtttccg cctatgtttt
     3841 cggtattgca cttgtattcc tgattgtttc tctttccttt tctgtttggg aaattcagat
     3901 ttctaccggt tcgctcaaga tttatttgga ggaaatggaa aacgaagaaa tcagggagga
     3961 agaaaaactt aaaacttgaa gaaatggaaa caaattttgg ttttgtccgc atagcagctg
     4021 cggttcccga actcaaagtt tcggatattt tgtttaacac tgaaaaaata atagaactga
     4081 ttcatgttgc cgaagaaaac cgagcccaag tggtctgttt ccccgaattg tgtatcaccg
     4141 gttacacgtg cgccgattta ttttttcaac aacagttgca ggaagaagta ttgaaagccc
     4201 tgtctgaaat tcagatggcc accttgtccg tttcctgtgc tgttatcgtg ggagcaccgc
     4261 ttcgttttcg caatcggctt ttcaatacgg cggtcgtctt acaaaaaggg cggattatcg
     4321 gcatcgttcc taaaactttt ttgcccaatc atggcgaatt ttacgaaaag cgatggtttg
     4381 tttccggaaa aaatcttcgt ggacaaacta ttgtgtgcaa tgaacaggaa gtgccttttg
     4441 gcgtcgattt aatatttttt tctccttcat tttcttttgg aatcgaaatt tgtgaagatt
     4501 tatgggcacc gcttcctccc agtaccgaat tgtgcatggc aggggcagaa attattttta
     4561 atccttcggc cagcaatgaa ttgattggaa aacacgaata tcgactgcaa cttattgaac
     4621 aacagtcggc tcgttgccat gccggatatg tttattcttc ttccggaatg tacgaatcga
     4681 ctaccgactt ggtcttttca ggaagtgcag tcattgcaga aaacggaaaa attctcgaaa
     4741 cttccaaaag attttcgctc gattctcagc tgattttcaa cgaaatagat gtcgaaatat
     4801 tgcggtacga aagaatgcac aacagtaatt tcggtgaaga tgctttacag cagacttaca
     4861 ggaaaattga aatgcagggg caacctctcg aacagaagga aattaaaaga acggtttcac
     4921 cctacccgtt tgttccacct gtatcgaaaa gagaagaaag ctgcagcgag attttttcca
     4981 ttcaaacgtc ggggttggcc aaacgctggc tgcatacaca agccaaatcg ctgatagttg
     5041 gaatttccgg agggctggat tctacgcttg ctttgctggt gtgtgtaaaa actgccgaca
     5101 aattgggttt tgacagaaaa actgttattg gggttaccat gcccggtttt ggcacaacca
     5161 accgaacgta tggcaatgcg ttgaatttga tgaaatcttt ggaaattacc ggtttggaaa
     5221 tttccatcaa ggaagcttgt ctgcaacatt tcaaagacat tggacacgac ccggatgttc
     5281 acgacgtgat ttttgaaaat acacaggcac gtgaacgaac gcaaatttta atggatttgg
     5341 caaataaata caacggattg gtgattggaa ccggcgacct ttcggaattg gcgctgggat
     5401 gggcaacata caacggcgat cacatgtcga tgtatgccgt taacagcggt attcccaaaa
     5461 ctttggttcg ttatctggta gaatatgtgg ctcaaagtat ggaagaaaaa accaaaacaa
     5521 ttttggaaga tattttgaaa acgcctgtta gtcctgaatt attaccagct gatgagtatg
     5581 gagaaattca tcaaaagacg gaagatttgg tgggtcctta cgagttgcac gatttctttc
     5641 tttactattt tgttcgtttt ggcttcagtc caaaaaaaat tctttttttg gcgcaaaaag
     5701 catttggcga taaatattcg gaagcaatcc tgcggaaatg gctaaaagtt tttttacggc
     5761 gtttttattc gcaacaattc aagcgttcgt gtatgcccga tggcccaaaa gtcggctcca
     5821 tcaatctttc gccgcgtggc gactggcgga tgccgagcga tgccggtttt cattgggacg
     5881 atataatttg atttctttat aaacactgtt tcatttcata aatttaaaat tcatgttatt
     5941 attttttgaa agaataattt tgggaaaccg actgtccgat tggggaatct ctctgttgat
     6001 aattgtgggt gcatttttgt tgaataaagg tatttcactt ttgaacaaaa acgtaattca
     6061 gaaaattacg gccaaaagta aaaatcggct ggacgattta ttggtaaaaa cactcgaagc
     6121 gcctattcta cttggtattg ctttgattgc cgtatgggtg gctgctcgcc gtctcgattt
     6181 gggtacacag gtcgaccaag gcttttatcg cgcttatcag atattgacgg ttcttaatat
     6241 cacatggttt ttggtaaaat taaccggtgc cctgatcaat gaatatcttc tgccgcactc
     6301 aaaggaagag gaagataaca aaaggctcga tgaaagcatg gtcagaattt tacgacgcat
     6361 ttcggtgggc attatttggg ctattggcat tgtgatggca ttgaacaatg caggtgtcaa
     6421 tgtgggagct ctgctggcca gtttgggaat tggaggtttg gcagtggctt tggcggcaca
     6481 ggacacgtta aaaaattttc ttggcggact caccattttt atagatcggc cctttcgcat
     6541 aggcgatcgg attgttaccg gagcctatga cggttttgtt gaagatgttg gcattcgcag
     6601 caccagaata cgaaatttcg acagaagaat catatccatt cccaattaca aaattgtaga
     6661 tgccgccata gaaaatattt ccattgaacc catgcggcgc gtagtgctga aaatcggcct
     6721 tacttacaac actcctcctg aaaagatgaa agaagccatt actattttga aaaatattcc
     6781 gcaaaaagtt gatgaggtgt ttccagatga aatttatgct tatttcaccg acttttccga
     6841 ttcggcactg acaataacat ttatttactt tattaaaaaa gaggcaaatg tgatggaaac
     6901 accctcaaag gtaaatatga atattttgga agatttcaat gcagcaggtc taaactttgc
     6961 tttccctacg cgtactgtgt acattgaaaa tcaaaactga caatcggaat ccaaaagacc
     7021 ggtaattttg tggtagctta atggtgaact cgtttaagat acaaataaga acatgaaaaa
     7081 aacggaaacg gcacagaagc atctttagaa aaacaatcgc tcggcaagct cg
//
LOCUS       DAOTAX010000080         7055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9025_length_7055_cov_28.477429, whole genome shotgun sequence.
ACCESSION   DAOTAX010000080 DAOTAX010000000
VERSION     DAOTAX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9025_length_7055_cov_28.477429"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..712
                     /locus_tag="PLM04_07565"
     CDS             188..712
                     /locus_tag="PLM04_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HON02967.1"
                     /translation="MAHIQSVRGIDPKIGKNCFLAETAVVIGDVTMGEGCSIWFNAVL
                     RGDVNSIKIGNHVNIQDGSVLHTLYQKSIIEIGDYVSIGHNVTIHGAKINDYALIGMG
                     AVVLDYAEIGEGAIVAAGALVLSNTVVPPYTLWAGVPAKFIKNVEPKQTNEINHRIAQ
                     NYSLYASWYQDKDK"
     gene            complement(973..1764)
                     /locus_tag="PLM04_07570"
     CDS             complement(973..1764)
                     /locus_tag="PLM04_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HON02968.1"
                     /translation="MRKYIFLSCLTLLLVSCSSVLITGRKQLNFVPEREILSMSSQSY
                     KEFIASAPLSSDKTNTALVKKIGSNIASAVEIYLRNNGLASEIANLSWEFNLVKDTSK
                     NAFCMPGGKVVVFEGILPIAKNEDGLAVVMGHEIAHAIAKHSNERLSQQMLAQFGAEL
                     TNILLSNKSEATRIGINTLYGIGVEMGVMLPYSRKQEYEADRLGLIFLAMAGYNPQES
                     VAFWQRMAAASSGSNIEFLSTHPSDQNRIKKLQEFIPEAMKYYNK"
     gene            1872..2300
                     /locus_tag="PLM04_07575"
     CDS             1872..2300
                     /locus_tag="PLM04_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HON02969.1"
                     /translation="MQHFQKKKIFFVKYWKSLLVLFVILYLSFASPSTFKDIPTFQHA
                     DKVAHFLMFALFTGVLINDFRHDKIVFTSKTEFLILSLAFPLFTGAIIEYLQGSVFQP
                     RSAEWLDFFSDTMGVVIGFLLMSALFHFLKKIKKIIRKDG"
     gene            2293..3408
                     /gene="dprA"
                     /locus_tag="PLM04_07580"
     CDS             2293..3408
                     /gene="dprA"
                     /locus_tag="PLM04_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HON02970.1"
                     /translation="MDDSLKYKIALTMIHGIGNILAKNLIAYIGSVEGIFKEKRQNLA
                     KIPGIGEVLSKEIVSHSLIERAEKEIEFISKNQIKAIFFTEKDYPYRLKECPDSPILL
                     YCKGNTNFNEGKFVGIVGTRSATQTGKENCTKLVSDLAAMVPQLTIVSGLAYGIDIYS
                     HKAAVENHLPTIGVLGHGLDRIYPAAHRSTAIKMLAHGALLTEFISETNPDKPNFVKR
                     NRIIAGLCDALVVVESGIKGGALITAELANDYNRDVFAFPGRVDDIWSKGCNSLIKSN
                     KAFLMETAEDLLQIMNWEKLDSGSEIAPQVSMYFDLAEEEKEILFQLRQHADGLQVNE
                     LAIQMSKPFSKISSLLLQMEFKGLVKCLPGNIYRIIK"
     gene            3706..4140
                     /locus_tag="PLM04_07585"
     CDS             3706..4140
                     /locus_tag="PLM04_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HON02971.1"
                     /translation="MALVKFSDQLPSLFDRLMNNDLFDWTFRNFSETNTTLPSVNIKE
                     TNDAYRLEMAAPGLKKEDFKIEVKDGVLSISSEKVGEELKEGEEFTHREYSYQSFVRS
                     FTLPDNVEEDKIEAKYENGILCVNIPKKEEAKAKPVKQIEIQ"
     gene            4229..5170
                     /locus_tag="PLM04_07590"
     CDS             4229..5170
                     /locus_tag="PLM04_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HON02972.1"
                     /translation="MEYKDYYKTLGVDKNATQEEIKKAYRKLAVKYHPDKNPGNKEAE
                     NQFKMINEAYEVLSDPEKRKKYDELGTNWNKFGEGNEYRGSNPFEGFGNRGGSQFYYQ
                     DNFNDLFGGKNTGFSDFFEAFFGKGATSGFGKSNRQTEFKGQDYETEINLTLEEAYHG
                     TSRIIQIDNEKIRIKIKPGVNDGQVLRIKGKGAKGSNPNYNGDLLIKVKVLPHPIFER
                     IGNDLKMIHQINLFDAILGGKTIIETLNGKIIIDIPEGTQNNKILRIKGKGMPVYGKS
                     GEYGDLLIKLQVIIPENLTPEQKNMFRRLKENLHSQK"
     gene            5188..5544
                     /locus_tag="PLM04_07595"
     CDS             5188..5544
                     /locus_tag="PLM04_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02973.1"
                     /translation="MYDLNRFFPEDNEMLTNFVEALFSANLQYSAMDLFRAGAESWKE
                     IDGAVKLSIQTLIKANLNPQMHFKFRFLTDIASGNIVQDWKMSKTGFVLTLLNIQGKN
                     PLLNKLKVEIIERYIN"
     gene            6126..>7055
                     /gene="argC"
                     /locus_tag="PLM04_07600"
     CDS             6126..>7055
                     /gene="argC"
                     /locus_tag="PLM04_07600"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HON02974.1"
                     /translation="MKKIKVGIVGGAGYTAGELLRILIYHPEVEIVFVNSSSNAGNPL
                     AEVHSGLIGETDLKFTAELPLQQIDVLFLCTAHGDSKKFLVEHDVPFSVKIIDLSTDF
                     REKRTDHDFVYGLPELNRKEILSARHVANPGCFATAIQLGLLPLAKNGLLKDEIHVNA
                     ITGSTGAGVKLSSTSHFSWRNNNVSIYKAFTHQHLHEINQSLHQLQPSFAHAVNFIPV
                     RGNFTRGILAASYTKCDLTLKEATEIYREFYSEAAFTFVVDKNPDLKQVVNTNKALIY
                     LEKHEDKLLVVSVIDNLLKGASGQAVQNMNLMFG"
BASE COUNT         2449 a         1141 c         1336 g         2129 t
ORIGIN      
        1 aacagctctt ctgagtttga cttttctttt aaattggcta atgagtaatc tgggttgaac
       61 aacagattaa gaaaaaaatt aaaattatta acatcaaatg aatttttatt acgaaatttg
      121 tctaacgcta aagttgcatt aataaattga attcagtttg aaatttcact tgaaaaataa
      181 aatatacatg gcacatattc aatcagtcag ggggattgac ccaaaaattg gaaaaaattg
      241 ttttttggca gaaacggcag tagtgattgg cgatgttaca atgggagaag gttgtagcat
      301 ttggttcaat gcagttttac gtggcgatgt caattccatt aaaattggaa atcatgttaa
      361 tatccaagat ggatcggttt tgcacacgct gtatcaaaaa tcgattattg aaatagggga
      421 ttatgtttca attggacata atgtaaccat tcatggggca aaaatcaacg attatgcatt
      481 aataggaatg ggagcggtgg ttttggatta tgctgaaata ggagagggag ccatagttgc
      541 tgccggagct ttagttttaa gcaatactgt tgttcctcct tacaccttgt gggctggcgt
      601 tcctgctaag tttataaaaa atgtggaacc taaacagact aacgaaatca atcatcgcat
      661 tgcccaaaat tattcactct atgccagctg gtatcaagat aaagataaat aataatctct
      721 cttgccataa agctacaaaa aaaatttcag catttggcaa cttttaagct catcaaaatg
      781 ccgtattaat gttcaaatag ttaataattt tttttggaat ttgaaagatg agcaaacatc
      841 taagaattta aagaaaaaaa ttttttgaga tatccatcga aaggtgcatc aaacactaaa
      901 ttctttcttt tcagattaac caagtatctg cgcttaaata cataatttta ctggtgaatc
      961 atgatatcac aatcatttgt tgtaatattt catggcttca ggaataaatt cctgaagttt
     1021 ttttattcga ttttgatcgg aagggtgcgt acttaaaaat tctatgttgc tgccagaaga
     1081 agcagcagcc attctttgcc agaaagccac agattcttga ggattgtatc cggccatagc
     1141 caaaaaaatc aaaccaagcc tgtcagcttc atattcctgt tttcttgaat aaggcaacat
     1201 cacacccatt tctaccccga ttccataaag ggtattaatg ccaatccgtg tagcttccga
     1261 tttattggac aacaaaatat tggttaattc ggctccgaat tgtgccagca tttgctggct
     1321 gagacgttcg ttgctgtgct tggctatggc atgtgcaatt tcgtgtccca ttactacagc
     1381 caacccatct tcattttttg ctatcggtaa aatgccttca aataccacca cttttcctcc
     1441 cggcatacaa aaagcatttt ttgaagtatc ctttaccaaa ttaaattccc aagaaaggtt
     1501 tgcaatttca gacgccaaac cgttatttct caaatatatt tcaacagcag atgcaatgtt
     1561 gctgcctatt ttttttacta atgcagtatt tgttttgtcg gatgacaaag gagctgaagc
     1621 tataaattct ttataacttt ggctactcat tgataaaatt tctctttccg gaacaaaatt
     1681 caattgcttt ctgccggtga tgagaacact tgaacaggaa accagcaaaa gagtcaaaca
     1741 acttaaaaaa atatattttc tcataataat gatatttgaa aaacaaattt taatattttg
     1801 agaacaggtt ttatctttgc aaagataatc aaaatttaat tattttttct tgataaaatt
     1861 attttagttg tatgcaacat ttccaaaaaa agaagatatt tttcgttaaa tattggaaat
     1921 ctttattggt attgtttgta attttatatc tgtcgtttgc cagcccctct acctttaagg
     1981 atattcctac atttcaacac gccgataagg ttgctcattt tcttatgttt gccctattta
     2041 caggggtttt gataaatgat tttcgacacg ataaaattgt tttcacttcc aaaaccgaat
     2101 ttttaatatt gagtttggca ttcccattat tcaccggagc tattattgaa tacctgcaag
     2161 gttcggtttt tcaaccgcgc tcagcagaat ggctcgactt tttttctgat acaatgggag
     2221 tagttatcgg atttctttta atgtctgcat tgtttcattt tttgaaaaaa ataaaaaaga
     2281 ttatccgaaa agatggatga ttccttaaaa tataaaattg ccctaacgat gattcatggc
     2341 ataggaaaca ttcttgccaa aaatctgatt gcatacattg gaagtgtgga aggaattttt
     2401 aaggaaaaaa gacaaaacct ggcaaaaatt cccggtattg gagaggtttt gtccaaagaa
     2461 attgtatcac acagccttat tgaacgagca gagaaagaaa ttgaatttat ttctaaaaat
     2521 caaatcaaag ccattttctt cactgaaaag gattatccct atcgattgaa ggaatgtccc
     2581 gattctccaa ttttgttgta ctgtaaagga aacaccaact ttaacgaagg aaaatttgtt
     2641 ggaatagttg gaacacgaag tgccactcaa acaggaaaag aaaattgtac caaacttgta
     2701 tccgatctgg ccgcaatggt acctcaactc accatagtga gcggactggc ttatggtatt
     2761 gacatttatt ctcataaagc ggctgtagaa aatcatttgc ctaccattgg tgttctgggt
     2821 cacggattgg ataggatata ccctgctgca catagatcta cggctatcaa aatgcttgct
     2881 catggagctt tgctgaccga atttattagt gaaacaaatc cagataaacc aaattttgta
     2941 aagcgtaacc ggattatagc cggactctgc gatgctttgg tggtagtaga atcgggtatt
     3001 aaaggcgggg cattgataac ggccgagttg gcaaacgatt ataacaggga tgtatttgct
     3061 tttcccggcc gtgtcgatga tatttggtcc aaaggatgta acagtctgat aaaatcgaat
     3121 aaggcatttt taatggaaac agcagaagac ttgcttcaaa taatgaattg ggaaaaactg
     3181 gattccggtt cggaaatagc tccgcaggtt tccatgtatt tcgatctggc agaagaagaa
     3241 aaagaaattt tgtttcagtt gagacaacat gccgatggac ttcaagtaaa tgaactggct
     3301 atccaaatgt ccaaaccatt cagtaaaatt tcttcgctgt tgctgcaaat ggaatttaaa
     3361 ggattggtaa aatgtttacc gggtaatatt tatcgaataa tcaaataatt ctgataaaaa
     3421 agagaagaaa ttagtggttg tggtgaaaca agtaattgtc tatcagatat agtcaagtaa
     3481 aaaacaaaca ttgacagttt ggcattacag cgtaaaaata tgtcattctg tcaataattt
     3541 tattcccttc ttcgattact tctgcaacca taagataaaa aattactctg aaatacagtt
     3601 atttaccggg caaattaaat tattggaaat gctctggtat tgaatttgaa tagcttgagg
     3661 tgtagagtaa aatttagaat tataaaacga aaggagggtt taactatggc attagtaaaa
     3721 ttttcagatc aattgccatc attatttgat cgtttaatga ataatgattt gttcgactgg
     3781 acttttcgca acttttctga aacaaacaca actcttccat cagttaacat taaagaaacc
     3841 aacgatgcat acaggttgga aatggcagct ccaggtctaa aaaaagaaga cttcaagatt
     3901 gaagttaaag atggggtgct ttcaatttct tcagaaaaag ttggtgaaga actgaaggaa
     3961 ggagaagaat ttacccacag ggaatacagc tatcaatcat ttgtacgttc ttttactttg
     4021 cccgacaatg tagaagaaga caaaatcgaa gcaaagtacg agaatggaat attatgcgta
     4081 aatattccca aaaaggaaga agctaaagct aagccggtta agcaaatcga aattcaataa
     4141 attttacttt aacaaatgaa ggggaactat tgtgttcccc tttattaaat ttattttaac
     4201 aatttgttgc ttgaaatatt tgttttatat ggaatataaa gattactata aaacacttgg
     4261 agtagacaaa aatgccactc aggaggaaat taaaaaagct taccggaaac ttgccgtaaa
     4321 ataccatcct gataaaaatc ccggtaacaa ggaagcggaa aatcagttta aaatgattaa
     4381 tgaagcttac gaagtactgt ccgatccgga aaaaaggaaa aaatatgatg aattgggaac
     4441 caactggaac aaatttggtg aagggaacga atatagagga agtaatcctt ttgaaggttt
     4501 tggcaatcgg ggcggaagtc aattttacta tcaggataat ttcaacgatt tatttggtgg
     4561 taaaaacact ggattttcgg attttttcga agcatttttc ggtaaaggag caacgtcagg
     4621 atttggtaaa agtaatcgtc aaaccgaatt caaagggcag gattacgaaa cagaaatcaa
     4681 tcttaccctg gaagaggcat atcatggtac aagcagaatt attcaaatcg ataacgaaaa
     4741 aatacgtata aaaatcaaac caggagttaa cgatggacaa gtattaagaa taaaaggaaa
     4801 gggagcaaaa ggaagcaatc caaactataa tggcgatttg ctcataaaag tcaaagtact
     4861 accccatcca attttcgagc ggataggcaa tgacttgaaa atgattcatc aaattaactt
     4921 gtttgatgca atactgggtg gaaaaacaat tattgaaaca ctgaatggaa aaataataat
     4981 tgacattccc gaaggtacac agaacaataa aattctgcgt atcaaaggaa aaggtatgcc
     5041 tgtgtatgga aaaagtggag aatacggcga tttgctgatt aaattgcaag tgattattcc
     5101 cgagaacctt acacccgaac aaaaaaatat gtttcgtaga ttaaaggaaa atttgcattc
     5161 acaaaaataa taattaatag atggattatg tatgatttga acagattttt ccccgaagac
     5221 aatgaaatgt tgacaaattt cgtagaagct cttttttctg caaatcttca gtatagtgca
     5281 atggatttgt ttcgagcagg agctgaatca tggaaggaaa tcgatggggc agtaaaacta
     5341 tccatacaaa cgcttataaa agccaatctg aatccgcaga tgcattttaa atttcgattt
     5401 ttaaccgata tagcaagtgg caacatagtg caagactgga aaatgagcaa aacgggtttt
     5461 gtattgacat tactcaatat tcagggtaaa aatccacttt taaacaagtt aaaagtagaa
     5521 attattgaac gctatatcaa ttaaatgaag aaggaaaaac agatgttttt tcgacattgc
     5581 gaataaaaaa ccgatatcta aaaattggaa aactgtatca tatttaacgc atatcaattg
     5641 aggataagta tttgtggaaa aattcgaaat ttctgaaaaa acggataaag ttagctcatg
     5701 acagatattg ccaatatatt ttttaagtgt ggatggaatt tgcactatag attattaaaa
     5761 aagttcatac tatcaatgca ttttgactga atattattac caagaaaaaa agtgttggga
     5821 acataaatca gatggcaatg tcttaatttc catatctccc ctaaataaaa aatcgtaaat
     5881 tcaagtgaaa attaaacttt ttggtgcatc attaccaata aaatgttaac tttgaaacct
     5941 gttttaaaac ggtttttcag gtaaacagag aggaataaag caaatttttt aagaacaaaa
     6001 attttgtaca tgacaaaaaa gtaagtaggg tttggggcaa agccccgtta ttaaatattt
     6061 gaaaataaaa aatataaatt tttctacaat ttttttgtca tgtactaagg aacaaaaatt
     6121 tcaatatgaa gaaaattaaa gtcggcatag tgggaggggc aggatacacg gcaggcgagt
     6181 tgttgcgcat tctgatttat catcctgaag ttgaaattgt gtttgtaaac agcagcagca
     6241 atgccggtaa tcctttagca gaagtgcata gcggattaat aggcgaaacg gatttgaagt
     6301 ttactgcgga gttgccacta cagcaaatag atgttctgtt tttgtgcaca gcacacggcg
     6361 acagtaaaaa attccttgta gagcatgatg ttcctttttc ggtgaaaata atcgatcttt
     6421 ctaccgattt tcgtgaaaaa agaaccgatc acgattttgt ttacggttta cccgagctga
     6481 accgaaagga aatactttct gcccggcatg tagccaatcc cggttgtttt gctacagcca
     6541 ttcaattggg actgttgcca ttggcaaaaa acggcttgtt gaaagatgaa attcatgtca
     6601 atgccattac cggttctacc ggagccggcg taaaactttc ctccacttct cattttagct
     6661 ggagaaacaa caatgtttcc atttacaaag cttttacgca tcagcatttg cacgaaatca
     6721 accagtcgtt gcatcagttg cagccctctt ttgcacatgc tgtaaatttc attccggtgc
     6781 gcggaaattt tacgcgcggc atactggcgg caagctacac caaatgcgac ttgacactca
     6841 aagaagccac tgaaatttat cgcgaatttt acagcgaggc cgcttttaca tttgtcgtag
     6901 ataaaaatcc ggatctcaaa caagtggtaa acaccaataa agcattgatt tatctggaga
     6961 aacatgaaga taaattactt gttgtatcgg ttatagacaa tttgctgaaa ggggcttcgg
     7021 gacaggcggt gcaaaatatg aatctgatgt tcgga
//
LOCUS       DAOTAX010000081         7027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9080_length_7027_cov_31.095955, whole genome shotgun sequence.
ACCESSION   DAOTAX010000081 DAOTAX010000000
VERSION     DAOTAX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9080_length_7027_cov_31.095955"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1437
                     /gene="nusA"
                     /locus_tag="PLM04_07605"
     CDS             181..1437
                     /gene="nusA"
                     /locus_tag="PLM04_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HON02975.1"
                     /translation="MSKKETVNLIDTFSEFKELKNIDRSTLISVMEESFRNIIAKNYG
                     TDKNFDVIINPDKGDFEIYRNRTVVADDEVSDPNLEISLSEAQKIDEDYQIGEEVTDT
                     VDISDFGRRAILTLRQTLQSKILELQKDNVYTKYSKMVGQIVTAELYQIWKKEMLLMD
                     EEGNELYLPKSEQIPTDFYRKGDMVKAVVAMVENKNNNPRVILSRISPVFLERLFENE
                     IPEIHDGLITIKKIARIPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNEN
                     IDVINYTNNISLFISRALSPAKISSIRINEAEKKAEIYLKPEEVSQAIGKGGLNIKLA
                     SMLTGYTLDVFRELDETETEDIYLDEFNDEIDQWVIDTLKSIGCDTAKNVLAIPREDL
                     IRRTDLEEETIDEVLDILRSEFEDEN"
     gene            1502..4555
                     /gene="infB"
                     /locus_tag="PLM04_07610"
     CDS             1502..4555
                     /gene="infB"
                     /locus_tag="PLM04_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HON02976.1"
                     /translation="MPIRLSKACKDLNVGMSTAVEFLAKKGHKLSADPNLKLEDELYL
                     LLAKEFNKDMALKIESERISKERQEKEKAETVGLEEYVHPKTAPKPAEIIPTVIPEEQ
                     KPHIKSVGHIDLEETGKKVKTKEKEKTEIVDSIPVEPIKEPVVAEKEKIEPDATFTHE
                     VKAVVTEEPVKPEKPEEIIQEQKSQPEEFEVTEKILPDSTEIKAEKIEEKIETQVITS
                     PEISKHRTTQESESSNLEEVNVNEAEIEISELTEEVFYTEIPVLDTNITVVGSIDLDA
                     LNQRVRPKPKSKEQRKREKEEKIQLEAEAKKIAQKGKKVIKLPGEAEDAEDTKDHHKK
                     KRERFTKQKVDISKVSGNANPEELERPKKLNLSPKGTKDKFKKSIKPAISEEDIQKQI
                     KETLARLATSNKKTKGSKYKKDKREIINARQQQLAEMEQSNEKVLKLTEFVTVNELAN
                     MMDVPVNDVISTCMNLGLIVSINQRLDAETINILADEFGFTTEYVSHDVIEALAENEP
                     DDEENLQPRPPIVTVMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIGAYNVVLDNGQ
                     RITFLDTPGHEAFTAMRARGAKVTDLAIIIIAADDNVMPQTVEAINHASAANVPIVFA
                     INKVDKPDANPEKIKEQLANMNYLVEDWGGKYQSQEISAKKGIGVPELLEKVLLEAEL
                     LDLKANPNRRAIGSIIESSLDKGRGYIATVLVQNGTLHQGDVVLAGTHFGKVKAMFNE
                     RNQRISEARPSEPVLILGLNGAPQAGDNFVVMESEHEARELALKREQLQREQTIRTKK
                     HFTLDELGRRIALGDFKELNVIVKGDVDGSVEAISDSLLKLSTEKIQVNIIHKAVGAI
                     SDSDVLLAAASNAIIVGFQVRPTPSARKMAEKEEIDIRLYSIIYDAIEEIKSAMEGML
                     SPEIKEEITATLEVRDVFKITKVGTVAGCIVKEGKIKRTNKVRLIRDGIVIFTGEIES
                     LKRFKEDVKEVAVNYECGVSIHNFNDIHVGDLIESFEEMEVKSEL"
     gene            complement(4824..5462)
                     /locus_tag="PLM04_07615"
     CDS             complement(4824..5462)
                     /locus_tag="PLM04_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HON02977.1"
                     /translation="MNIKTFIFNPFRENTYIVSDVTGEAIIIDAGCLTNREKQAVKNY
                     IENNKLVLKHLLNTHLHIDHQFGNRYIYDNYHISPEACKEDEFLIETMHEAAKFFGID
                     LHEEPPAIGSYIIENQEIKFGNSILKALHVPGHSPGSMAFYSEQNGLVFTGDVLFYGT
                     IGATHFEKGDHDLLIRSIWEKLMVLPDETLVYSGHGQPTTIGNEKNFNPYLV"
     gene            complement(5501..6123)
                     /gene="rsmG"
                     /locus_tag="PLM04_07620"
                     /pseudo
     CDS             complement(5501..6123)
                     /gene="rsmG"
                     /locus_tag="PLM04_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
     gene            6480..>7027
                     /locus_tag="PLM04_07625"
     CDS             6480..>7027
                     /locus_tag="PLM04_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HON02978.1"
                     /translation="MNHIPKLSHILVLILVVQLSSCITARKINYLQQPNASIPAYKDT
                     FSFNDYRLKKGDRVFIKIYSWDDKTNALLNGSSNIEQLVMSGSSSGNELYTYLIQPSG
                     KVNLPMVGEIYLEGQTVREAKETLEKFIQPVFDISSVDVRIAGRFFSVIGGGATGRYP
                     LNREKINIFEALAMAGDIDTYG"
BASE COUNT         2472 a         1185 c         1403 g         1967 t
ORIGIN      
        1 aaaaaaggag aaaaattttc cggaatactc acagaggtaa attttgataa ttgtacgctt
       61 caaattgaaa aattaatcaa acccgaaggt gcaaaaagga aaatcaaagt tacagaaaaa
      121 gttccttttc gatatgaaga tattaaaact acaaaatata ttttaagatt taaataagcc
      181 atgagcaaaa aagaaactgt taatttaatt gatacttttt cagaatttaa agagcttaaa
      241 aacattgatc gaagtacttt aatcagtgta atggaagaat cgttccgcaa tattatagca
      301 aaaaattatg gaaccgataa gaattttgac gtcatcatca accccgataa gggagatttt
      361 gaaatatatc gaaaccgcac tgtagtggca gatgatgagg taagcgatcc aaatttagaa
      421 atatcgctca gtgaagccca aaaaattgat gaagattatc aaataggaga agaagttacc
      481 gataccgttg acatttcgga tttcggtcga cgtgccatct tgactttgcg gcaaacgctg
      541 caatcaaaga ttctggagtt gcaaaaggat aacgtttata ccaaatacag caaaatggtt
      601 ggtcaaattg ttacggccga actatatcag atttggaaaa aagaaatgct tctcatggat
      661 gaagaaggaa atgagcttta tttgccaaaa tcagaacaga ttccgacgga tttttatcga
      721 aaaggcgaca tggtgaaagc tgtagtggct atggtggaaa ataaaaacaa caatccgcga
      781 gtcattcttt cccgtatttc gccggtattt ttggaacgac tttttgaaaa tgaaattcct
      841 gaaatacatg atggactgat aaccataaaa aaaatagcca gaattcccgg cgaacgcgct
      901 aaagttgctg ttgaatccta tgacgaccgc attgaccctg ttggagcttg cgtaggtatg
      961 aagggttcaa gaattcacgg aattgtgcgt gaattgcgaa atgaaaatat agatgtcatc
     1021 aattatacga ataacatcag tttgttcatt tccagagcat tgagtccggc caaaatttca
     1081 tctatccgca tcaatgaagc tgaaaagaaa gccgaaattt atctgaaacc cgaagaagtt
     1141 tctcaagcca tcggaaaagg agggctcaat attaagcttg ccagcatgct taccggttat
     1201 acactcgatg ttttccgcga attggatgaa accgaaacag aagatattta cttggatgaa
     1261 ttcaatgacg aaatcgatca gtgggttatt gataccttaa aatctatcgg ttgcgatacg
     1321 gctaaaaatg tattggccat tccacgtgaa gatttgattc gtcgcaccga tttggaagaa
     1381 gaaactattg atgaagtgct ggacatttta cgttcggaat ttgaagacga aaattgagaa
     1441 ttttttcaat aaatgaacaa aagttcaaag cttttaaagt taataattgt aaaataaata
     1501 tatgcctata agattaagta aagcatgcaa ggatttgaat gtcggtatga gtacggccgt
     1561 cgaatttctt gcaaaaaaag gtcataaatt gtcagccgat ccgaatttaa aattggaaga
     1621 tgagctgtat cttttactcg caaaagaatt taataaggat atggcgttaa aaattgaatc
     1681 ggaacggatc agcaaagaac ggcaggaaaa agaaaaagcc gaaactgtcg gattggaaga
     1741 gtatgttcat cccaaaacgg caccaaaacc tgccgaaata atacctactg tcatccctga
     1801 agaacaaaag cctcatatca aatctgtggg acacattgac ttggaagaaa cagggaaaaa
     1861 agttaaaacg aaagaaaaag aaaaaacgga aattgttgac tcgattcctg ttgaaccaat
     1921 aaaggaacct gttgttgccg aaaaagaaaa aattgaaccc gatgctactt tcactcatga
     1981 agtaaaagcg gttgttaccg aagaaccagt aaaaccggaa aaacctgaag aaataattca
     2041 ggaacaaaaa tctcaacctg aggaatttga agttacagag aaaattttgc ccgattcaac
     2101 agaaattaag gccgagaaaa tagaagaaaa aattgaaact caagtaataa cttctcccga
     2161 aatttccaag catcggacaa ctcaggaatc agaatcttcc aatttggaag aagttaatgt
     2221 taatgaagca gaaattgaaa tttcggaact aacggaagaa gttttttaca ccgaaatacc
     2281 tgttttggac accaatatta ctgtggtggg aagcatcgac cttgatgcac tcaaccagag
     2341 ggttagacca aaaccaaaat cgaaggaaca gagaaagagg gaaaaagaag aaaaaattca
     2401 gttagaagct gaagcaaaaa aaattgctca aaagggtaaa aaagttatta aacttcccgg
     2461 tgaagcagaa gatgcggaag atacaaaaga tcatcacaaa aagaaacgcg aacgttttac
     2521 aaagcaaaaa gttgatattt ctaaagtttc cggcaatgcc aatccggagg aattggaacg
     2581 tccaaaaaaa ttaaatcttt ctcccaaagg aaccaaagat aaatttaaaa aatccatcaa
     2641 acctgccatt tcggaagaag acatacagaa acaaatcaag gaaacccttg ctcgtttggc
     2701 aacttccaat aaaaagacaa agggttcaaa atacaaaaaa gacaaaagag aaattatcaa
     2761 tgcccgtcag caacagctgg cagaaatgga gcagagcaac gaaaaagtac tgaaactgac
     2821 ggaattcgta actgtgaatg aactggctaa catgatggat gttccggtca acgatgtgat
     2881 ttctacctgt atgaatcttg gtttgatcgt ttccatcaat caaaggttgg atgctgaaac
     2941 tattaatatt ttagccgatg aatttggctt cactaccgaa tatgtcagcc atgatgtgat
     3001 agaagcactt gctgaaaacg aacccgacga tgaagaaaat cttcaacctc gtccacctat
     3061 tgtaacggta atgggacatg tcgatcatgg taaaacatct ttgctggact acatccgcaa
     3121 aacaaatgtg atagccggag aagccggagg aattacccag cacattggcg cttacaatgt
     3181 ggtgttggat aatgggcagc gaattacttt tctcgacaca ccgggtcatg aagcgtttac
     3241 ggccatgcgt gccagaggtg caaaagttac cgacctggcc atcattatta ttgctgccga
     3301 cgataacgtg atgcctcaga ctgtggaagc cataaatcat gcttcggcag ccaatgtccc
     3361 cattgtattt gccatcaaca aagtggataa gcccgatgcc aatcccgaaa aaataaaaga
     3421 acaattggcc aatatgaatt atttggtgga agattggggc ggtaaatatc aatcgcagga
     3481 aatttcagca aaaaaaggaa tcggagtgcc cgaattgctt gagaaggtat tgctcgaagc
     3541 cgaattgctc gatttgaaag ccaatcccaa cagaagagca ataggttcta ttattgaatc
     3601 atcgttggat aaaggtagag gatatattgc tacggttttg gtacaaaatg gaacgctcca
     3661 tcaaggcgac gtagttttgg caggaacaca tttcggaaaa gtaaaagcca tgtttaatga
     3721 aagaaatcaa agaatctctg aagcacgacc ttcggaacct gttttgattt taggattgaa
     3781 cggagcacct caggccggag ataattttgt ggtaatggaa tcggaacatg aagcgcgtga
     3841 attggcattg aaacgtgaac agttgcaaag agaacaaaca attcgtacta aaaaacattt
     3901 tacactggac gaattgggaa gaagaattgc attgggcgat tttaaggaat tgaatgtcat
     3961 tgtaaaaggt gatgttgacg ggtcagttga agctatttcc gactcgctgt taaaactttc
     4021 taccgaaaaa attcaggtaa atattattca taaggctgtg ggtgccattt cggattccga
     4081 tgtgttgctg gcagcagctt ccaatgccat cattgtgggt tttcaggtac gtcctactcc
     4141 ttctgcccga aaaatggcag aaaaggaaga aatcgatatt cgactctatt ccatcatata
     4201 tgatgccatt gaagaaatta aatcggcaat ggaaggcatg ctctcgcctg aaattaagga
     4261 agagattacg gctactcttg aagtcagaga tgtgtttaaa attacaaaag tgggaactgt
     4321 agccggttgt atagtgaaag aaggaaaaat aaaacgaacc aacaaagttc ggttgattcg
     4381 cgatggtatt gttattttta ccggcgaaat cgaatcgtta aaaagattta aagaggatgt
     4441 aaaagaagtt gctgtcaact atgaatgcgg tgtaagtatt cacaatttca acgatattca
     4501 tgtaggagat ttgatagaaa gttttgaaga aatggaagtg aaatcggaac tgtaagccgg
     4561 caatatttct ctttaatgct gtcgaacact cagacaagaa tcatgaaaat tttgaaaaag
     4621 tcgccatgag ttagagaccg aaattaataa gataccgcag gtaagccgat gaaattatag
     4681 gacaaacaaa aaattcgaaa cgaagatagt ttcaaaaata gcagtctttt cctgcaattg
     4741 tcataaaatc aattgatgaa aaatctgcat tgaactgaaa aattttccgg aagatttgat
     4801 ttttttattg gaacaaactc ttattaaacc aaatacggat taaaattctt ttcgtttcca
     4861 atggtagtgg gttgtccgtg tccgctgtag accaatgttt catcgggtag aaccatcagc
     4921 ttttcccaaa tggaacggat taataagtcg tgatcgcctt tttcaaaatg ggtggctcca
     4981 attgtgccgt agaacaagac atctccggta aataccaaac cattttgttc tgaataaaaa
     5041 gccatgcttc cgggtgaatg gcccggaaca tgtaatgctt tcaaaattga gtttccaaat
     5101 tttatttcct gattttcaat aatataagag ccaattgcag gaggttcttc atgcaagtcg
     5161 ataccaaaaa attttgctgc ttcatgcatg gtttcgatta aaaattcatc ttccttgcat
     5221 gcttcaggcg aaatgtgata gttgtcgtaa atataacgat ttccaaattg gtggtcgata
     5281 tgcaaatggg tattgagtaa atgttttaaa acaagcttat tattttcaat ataattcttg
     5341 acagcttgtt tttcgcggtt tgtcagacaa cccgcatcaa taatgatggc ttcaccagtt
     5401 acatccgaaa caatataggt gttttctctg aaaggattga aaataaaggt ttttatgttc
     5461 atttttatat tctgtaaagg ttttgcctga ttaatttttt tcacacaaca ggcaaataaa
     5521 caattttttt gtctttaaaa tattcttctt taaaaaaatc ggaaatactg gctacttcaa
     5581 ttatcttttt gaaaggctta atttcttgtt ccaaactacc accctttaaa gaaataattc
     5641 cattgggtaa cgcattgatg gaggttttcc gaatattttt ttggtcattt tcagtaaatc
     5701 ttctagtggc atgactgcac gactgacaat aaaatcgtat tttccctttt cttcttctgc
     5761 tcgtaaatat ttgaattcgg aatttttcaa ctcaatggcc tcagcaaccg acattgcaac
     5821 tttaattttt tttcctatgg aatctatcat tacaaattta acttcgggaa aaagtatagc
     5881 aagcggaata ccgggaaatc ctcctccggt acccacatcc aaaatacttg agttcggctt
     5941 gaaacggata tattgagcaa tagcaagaga atgaagaaca tgatgttcgt acaggttttg
     6001 aatatctttt ctcgaaatca catttatttt ttcattccaa tccttataaa ggggatacag
     6061 cgcttcaaac tgagctttct gatgagatga caagtcggga aaatatttat tgatcaattc
     6121 cataaactca ttttttggca tacaaagtta agcgttttta caaaagtata aaatttcatt
     6181 tgatcgattc ttaatattca aggcaaaaaa ccaattgaaa aaataaataa gctcatctga
     6241 caatatatta acaaaaaggc ttcattgaaa ggatcaggat atcaaaatat ttagaaaaat
     6301 cttaaccgaa tacctcgaga taaattgaat tttaattggc tttaaaaaag agtttttcga
     6361 acatttttta atttcgaact tgccggttga ctctactaat ttctaatttt tttgtaatgt
     6421 tatatttctt ttgtaatttt gattaacttt gttgagtgtt ttaaaaagta gattttagaa
     6481 tgaatcatat tccgaagtta tcccatatac ttgttctgat tttggttgta caactttctt
     6541 cgtgtattac tgcccgcaaa attaattacc tgcagcagcc caatgcttcc attcctgcgt
     6601 ataaggatac tttttcattc aacgattaca gactgaaaaa gggggacagg gtttttataa
     6661 aaatctattc atgggatgat aaaacaaatg cattgctaaa tggtagcagc aatatagaac
     6721 agcttgtcat gtcgggttct tcatccggca acgaactgta cacttatctg attcagccct
     6781 ccggaaaagt aaatttgcca atggtaggag aaatatattt ggaaggtcaa actgtaagag
     6841 aagccaaaga aacgctcgaa aaatttatac aaccggtttt tgacatcagc agcgtcgatg
     6901 ttagaattgc tggaagattt ttcagtgtta ttggtggagg tgcaaccggc aggtatcctt
     6961 taaatcggga aaaaataaat atttttgaag cgctggcaat ggccggcgac attgatactt
     7021 atggcga
//
LOCUS       DAOTAX010000082         6976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9170_length_6976_cov_30.905360, whole genome shotgun sequence.
ACCESSION   DAOTAX010000082 DAOTAX010000000
VERSION     DAOTAX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9170_length_6976_cov_30.905360"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLM04_07630"
     CDS             complement(<1..779)
                     /locus_tag="PLM04_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON02979.1"
                     /translation="MKKTSVLRSVAIILATFFSSLLFAQGTIEGKVLDKKTKEPLVGA
                     SILIEGTSRGTTTDIDGNFKISELDPGKYKLIVSYVSYKKLEFNEIKIQKNIPTILNI
                     EMEEAELMLQSVQVVAQHRTDTELSLLKSVRTAVQVVNGISSQQIGKTLDRDAAEVVK
                     RIPGVTVQDDRFVVVRGLNQRYNNVWLNNASTPSSETDVKAFSFDAVPSNMIDNIMIY
                     KTGSPELSAEATGGFVKIFTKNIPEENFQQIEYSFGYNDQTT"
     gene            1245..2885
                     /locus_tag="PLM04_07635"
     CDS             1245..2885
                     /locus_tag="PLM04_07635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HON02980.1"
                     /translation="MKNFTQFGKFLLFLFFFFLVLLLIQAKATDFYFYVQFADKNNSP
                     YRLENPQEFLSERAIERRNFFSLSVDSTDLPVNPSYLSQVMNLGGEIHSQSRWLNGAT
                     VVMQDSALIEQVVSLPFVKWVQYTGKKATVSNVPKQPSKFSPAVDYGLSGSQIAQVKG
                     NFLHELGYFGKNIVVGVLDAGFLNVDINVAFDSLRLHNRLLGTKDFVGGSDLYRGNFH
                     GGNVLSIMAGNLPQNYLGTAPDASYWLIRTEDVDTEYLKELDFWVAGIEFADSVGSDV
                     VNSSLGYFTFDDPAMNFTYADMNGEVSRAAKAASMAAQKGMIVVCSAGNEGDKTWKYI
                     STPADAKGIITVGSVTADGTPSIFSSFGPSADGRVKPEICARGTQTSLVNSCGNIVFG
                     NGTSYSSPLICGLMACYLQYFKEQHKLISIPQLLQSVFVTGSLYSSPTDQMGYGIPDF
                     QLAFLSTAFNGLNNQTQNQPQWLVDEKNKTIQLNFAGKTDNSAISVQIHAMDGKLLLS
                     RFYRSSSIVLSIENLSSGIYLVSVENGGKIFSKKILVP"
     gene            2940..4187
                     /gene="xseA"
                     /locus_tag="PLM04_07640"
     CDS             2940..4187
                     /gene="xseA"
                     /locus_tag="PLM04_07640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HON02981.1"
                     /translation="MNENQNMTSLSLSELAAAIEETIRLNFDRPIWMRAEISELRENS
                     NGHCYMELIEKDENSDNLLAKVKATCWASVYRLLKPYFENATGQTFRAGLKVLLSVTV
                     EFHPVYGLSLTVRDIEPTFTIGEMAARRMQIIRRLEADGIADMNKQIPLPRLVQRLAV
                     VSSPTAAGYGDFCDQLKNNSGKFVFYTRLFPALMQGEQAESSIIAALEKIFRHAELFD
                     AVVIIRGGGATTDLSCFDSYELAVNCAQFPLPIITGIGHQRDVSILDLVAHSSLKTPT
                     AVAEFLIDRLQEAENLVKYAASDIYSFSKNYLDASLREINEKKLLIRQVLRNSLMRKQ
                     NEINRRISRLKTAVRMKLLREQNRLTLMEKSIETHSPAFMLKYGYSFTTLHGKKILSV
                     HEVKPGDRVQTYLHDGSFESELI"
     gene            4233..4601
                     /gene="folB"
                     /locus_tag="PLM04_07645"
     CDS             4233..4601
                     /gene="folB"
                     /locus_tag="PLM04_07645"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HON02982.1"
                     /translation="MAFIYLENMEFYSFHGCLEEERVKGNTFLVSVCMEVDTEKAGKT
                     DELSDTVNYQQVYDIVKKEMEIPSHLIEHVAQRIYDRIFEQIPQISGLHIRLSKLNPP
                     LGGKVEKASIEISGKRSDHC"
     gene            complement(4608..6527)
                     /locus_tag="PLM04_07650"
     CDS             complement(4608..6527)
                     /locus_tag="PLM04_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HON02983.1"
                     /translation="MKKILLITGIICGCNVFRFSGQVTTDTLSVQLSDVEINATRTKL
                     YPEMGRILTVIDKNEIERSAVRSIDELLDYVSGLDIRQRGKNGVQADISVRGGSFDQA
                     LILLNGINITDPQTGHFNLDIPIQLSDVSKVEILKGSSARMLGPNAFSGAINIITEEG
                     TKPFLKAGLEAGSFGYGEQSVTTAFIQKPYTLLSLSHKSSDGYTNNTDFENSNIFLHS
                     KLNSPKFGNIQFQAALQKKGFGANGFYSLAYPHQFESTQTLMAGLRWELIRRNISYHA
                     QSYWRQHHDRFELFRNFEGAPSSYTQHNYHLTDVVGGKFDASVANSWGKITFGIEGRN
                     EHIFSTVLGNEMNKTKPDPYENGINFNHSANRLLGTAFIDYAKIFDRWYVSAGGALSH
                     ATVFGTNPYGGFDIAFHPNNMTKIFFSSNTAMRLPTFTDLYYRSPTQISNPNLKPEHS
                     TTWELGAGIQQKHWQADATTFYRKGKNIIDWVKEPDSTKWESRNLTEVNAVGLDMNLN
                     YSFSNSFLQKAGLSYSFLQMDKNAENFDSKYALDYMKHKLIATLQHKTVKNCSLNWKL
                     SFLDRAGNYTDFYTQQPVNYQPYFLLDMKGLWEVEKYAVYLDLNNIFDKTYADYGGIV
                     QPGFNFTAGIKWKFK"
BASE COUNT         2007 a         1311 c         1380 g         2278 t
ORIGIN      
        1 gtagtctggt cattgtatcc aaaactgtat tcgatttgtt gaaaattttc ttccgggata
       61 tttttggtaa aaatttttac aaatcctccc gttgcttcgg cagaaagttc aggagaacct
      121 gttttataaa tcataatatt gtcaatcatg ttgctcggaa cagcgtcgaa ggagaatgct
      181 ttcacatctg tttcgctact tggtgtggaa gcattattga gccaaacatt attgtaccgc
      241 tgattgagac cgcgtactac tacaaatcgg tcgtcctgta ccgtaacgcc agggattcgc
      301 ttcactactt ctgccgcatc ccgatccagt gtttttccga tttgctggga agaaataccg
      361 ttaaccacct gaacggccgt gcgtacactt ttcagcaagg ataattccgt atcggtgcgg
      421 tgttgtgcca ctacctgtac actttgaagc attaattcgg cttcttccat ttctatgttt
      481 aaaatcgtag gtatattttt ttgaatttta atttcgttaa attcaagttt tttataagaa
      541 acgtacgaca ctatcagttt atattttccg ggatcaagtt cgcttatttt gaaatttccg
      601 tcaatgtcgg tagttgttcc ccgtgaggtt ccttcaatta gaatactggc acctaccagt
      661 ggttctttgg tttttttgtc taaaactttt ccttctatcg taccttgagc aaacagaagc
      721 gaggagaaaa atgttgctaa aattattgca acagagcgta atactgaagt ttttttcatc
      781 tgatttttct tctgaatttt tatgctgcaa aattaattca caactgtttc aaaattgata
      841 aaatgatatt acaatgcggt tataatgatt ttttgaaaga atattgaaat accttttcta
      901 ttttaacatg agtttgagat aagtatattt tataagcaat tttaaataag ctataaaggt
      961 tgttgaaaag taagtattca cattttatct aaggttagtt gtttataaat aaggttttta
     1021 ttttcagaaa gtgaacctta tttttttaaa taaaacctta gtaacgataa tcaacaacag
     1081 gaataaacct tattacctta ttaattatca atttattata tgattaaaac aggtaattgt
     1141 tctgtcgaac ttactataat taaaattttt ttgcaaagaa gcactttcaa aattcgtttt
     1201 attgaattat tccggtattt ttgtacataa aaaatccgta acatatgaaa aattttaccc
     1261 aatttggcaa attccttctt tttttgtttt ttttcttttt ggtacttcta ttgattcagg
     1321 caaaggcaac cgatttctat ttctatgtgc agtttgccga taaaaataat tctccttatc
     1381 gattggaaaa tccgcaagaa tttctttctg aacgcgcaat tgagcgacgc aatttctttt
     1441 cgctttccgt tgattcaacc gatttgccgg ttaatccttc ctatctctca caagtgatga
     1501 atctgggtgg cgaaattcat tctcaaagcc gatggttgaa tggagcaact gtggtcatgc
     1561 aggattctgc actgattgag caagtagttt cccttccgtt tgtaaaatgg gttcaataca
     1621 ccggaaaaaa agctacggtt tcgaatgttc cgaaacagcc ttcaaagttt tctccggccg
     1681 tcgactatgg actttccggt tctcaaatag ctcaggtaaa aggaaatttt cttcacgaat
     1741 tgggatattt tgggaaaaat attgtggttg gggtgctgga tgccggattt cttaatgtag
     1801 atatcaatgt tgctttcgac agccttcggt tgcataaccg attgcttgga acgaaagatt
     1861 ttgttggtgg ttcagatctg tatcgtggaa attttcacgg aggaaatgtg ctttcgatta
     1921 tggcaggaaa tttacctcaa aattatttgg gaacagctcc cgatgcttct tattggctta
     1981 tcaggaccga agatgttgac accgaatacc tgaaagaact cgatttttgg gttgccggaa
     2041 tcgagtttgc cgacagcgtc ggctcggatg ttgtcaattc gtctttgggt tattttacct
     2101 ttgatgatcc ggccatgaat tttacttatg ccgatatgaa cggagaggtt tcgcgtgcgg
     2161 ctaaagctgc ttcaatggct gctcaaaaag gtatgatagt agtttgtagt gccggcaacg
     2221 aaggcgacaa aacttggaaa tatatttcta caccggccga tgccaaaggc attattacgg
     2281 ttggaagcgt tacagccgat gggacaccca gcatcttttc ttcatttggc ccatccgccg
     2341 acggaagagt aaaacccgaa atttgtgcac gcggtaccca aaccagtcta gtaaattcgt
     2401 gtggcaacat cgtgttcggt aacggaactt cctattcttc tccacttatt tgcggattga
     2461 tggcttgtta ccttcaatat ttcaaagagc agcataaact gatttcgata ccgcaacttc
     2521 tacagtcggt tttcgtaacc ggaagcttgt atagttcgcc aacagatcag atgggatacg
     2581 gcatacccga ttttcaactg gcttttctta gtacagcgtt caacggattg aacaaccaaa
     2641 cccaaaatca accacaatgg ttggtggatg aaaagaacaa aaccattcag cttaattttg
     2701 cgggaaaaac cgataattca gctataagcg ttcaaattca tgcaatggat ggaaagttgt
     2761 tgctcagcag attttatcgt tcttcttcca ttgtcctttc cattgaaaat ctgagttcag
     2821 gtatttatct tgtttcggta gaaaatggcg ggaagatttt ttcaaagaaa attcttgttc
     2881 cctaaatttg tcaagaaaaa tatttctgtt gtatctttga agtgaaaaat tctcaatgaa
     2941 tgaacgaaaa ccaaaatatg acttctcttt ccttatctga actggctgct gctattgaag
     3001 aaaccattcg tctgaatttt gatcgcccca tatggatgcg tgccgaaatc agtgaattgc
     3061 gtgaaaattc caatggacac tgttacatgg aattaataga aaaggatgag aattccgaca
     3121 atttgctggc aaaagtcaaa gccacatgtt gggcttcggt gtatcgattg ttgaaacctt
     3181 actttgaaaa tgctaccggt cagacatttc gcgctggatt gaaggttttg ttgtccgtta
     3241 cggtagaatt tcatccggtt tatggtttga gccttacggt acgcgacatt gaaccgacgt
     3301 ttaccattgg cgaaatggca gcgcgcagaa tgcagattat tcgtcgtttg gaagctgatg
     3361 gcattgccga catgaacaaa cagattcctt taccgcgatt ggttcagcgt ctggctgttg
     3421 tttcttcacc cactgctgcc ggttatggag acttttgcga tcagctgaaa aacaattccg
     3481 gtaaatttgt gttttatacc cgccttttcc ctgccttaat gcagggcgag caagccgaaa
     3541 gctccattat cgcagctttg gaaaagattt ttcgtcatgc ggaacttttt gatgcagtgg
     3601 tgattattcg aggtggcggt gcaacaaccg atttgtcctg tttcgattcc tacgaattgg
     3661 cagtgaattg tgctcagttt cccttgccca tcatcaccgg catcggccat cagcgtgatg
     3721 tttctattct tgatttggtg gcacattcga gtttgaaaac acccaccgcc gttgccgaat
     3781 ttctcataga tcgcctgcag gaagccgaaa atctggttaa atatgcagct tccgatattt
     3841 attctttttc taaaaattat ctcgatgctt cacttagaga aatcaacgaa aaaaagctgt
     3901 tgattcgcca agttctgaga aacagtttaa tgcgcaaaca aaatgaaatc aatcgtcgga
     3961 taagtcgttt gaaaacggca gtacggatga aattgctcag agaacaaaat cgattgactt
     4021 taatggaaaa atctattgaa actcattctc cggcctttat gttgaaatat ggttacagtt
     4081 tcactaccct tcacggaaag aaaattcttt ccgttcatga agtgaaaccc ggcgaccgtg
     4141 ttcaaactta tctgcatgat gggagttttg agagtgagtt gatttgacgc gaaaaaataa
     4201 agggaattta ttaaatactc aattcttaac ttatggcatt catttatctc gaaaatatgg
     4261 aattttattc ctttcatggt tgcttggaag aagaacgtgt aaaaggaaat acttttctgg
     4321 tttcggtttg tatggaagtt gatacggaaa aggctggaaa aacggacgaa ctttcggata
     4381 cggtgaatta tcagcaggtg tacgatattg taaaaaaaga gatggaaatt ccttcccatc
     4441 tcatagaaca tgtcgctcaa aggatttacg accggatttt tgaacaaatt ccgcaaattt
     4501 ccggattgca tatccgactt tccaaattaa atcctccttt gggtggaaaa gtagaaaaag
     4561 cttcgataga aatttccggg aaacgttctg atcattgttg atcgttgtta tttgaacttc
     4621 cattttatcc cggcagtgaa gttaaatccc ggttgtacaa ttccgccata atccgcgtaa
     4681 gttttgtcga aaatattatt cagatcaagg taaactgcat atttctctac ttcccataat
     4741 cctttcatat ccagtaaaaa ataaggttga taatttacgg gttgttgcgt ataaaagtcg
     4801 gtgtagtttc ctgctcgatc caaaaaggag agtttccagt tgagagaaca gtttttcact
     4861 gttttatgtt gtaaagttgc tatcagcttg tgtttcatat aatccaacgc atatttggaa
     4921 tcgaaatttt cggcattttt atccatctga agaaaagaat aggaaagccc ggctttttga
     4981 aggaaagaat ttgaaaaaga atagttcaaa ttcatatcca gccccacagc gttaacttca
     5041 gtcaaatttc ggctttccca ttttgtcgag tcgggttcct ttacccagtc gataatgttt
     5101 ttcccttttc ggtagaaggt cgttgcatca gcttgccagt gtttttgttg aatgccggct
     5161 cccaattccc atgttgtgga atgttcaggt tttaaattgg gattgctgat ttgcgttggg
     5221 cttcggtaat ataaatcggt aaaagttggt aacctcatgg ccgtatttga tgaaaaaaat
     5281 atcttggtca tgttgtttgg atgaaaagca atgtcaaatc cgccatacgg attcgttccg
     5341 aagacagtgg catgtgacaa tgcccccccg gccgaaacat accatcgatc aaaaattttt
     5401 gcataatcaa taaatgccgt ccccagtaaa cgatttgcac tatgattaaa atttattccg
     5461 ttttcgtaag ggtctggttt cgttttattc atctcatttc ccaaaactgt gctgaaaata
     5521 tgttcatttc ttccttcaat accaaaagta atttttcccc atgaatttgc caccgaagca
     5581 tcaaattttc ctcccacaac atctgtcagg tgataattgt gttgtgtgta cgacgaagga
     5641 gccccttcaa agttgcgaaa cagttcaaat cggtcgtgat gttgtcgcca atagctctga
     5701 gcatggtagc tgatgtttct tcggataagt tcccatctca accccgccat cagggtttgc
     5761 gtactttcaa actgatgcgg ataagccaac gaataaaatc cgtttgcacc aaaacctttt
     5821 ttttgtaaag ctgcttgaaa ctgtatatta ccaaatttcg ggctattcag tttcgagtgc
     5881 agaaaaatgt tgctattctc aaaatctgta ttgttggtat aaccatcgct gcttttgtga
     5941 gataaagaaa ggagcgtata aggtttttgg ataaatgcgg tagtaaccga ctgttctcca
     6001 taaccaaaac ttccggcttc caaacctgct tttaaaaaag gttttgttcc ctcttcggtt
     6061 attatgttaa tggcaccgct gaaagcattc ggccctaaca ttcgggctga agaacctttt
     6121 aaaatttcaa ctttactcac atcggagagt tgaatgggaa tatcaaggtt aaaatgtccc
     6181 gtttgagggt cggtaatgtt gatgccattc agcaaaatta gcgcctggtc aaatgagcct
     6241 ccacgaaccg agatatcggc ttgaacgcca tttttgccac gttgacgaat atctagaccg
     6301 gaaacataat cgagcaattc atcaatgctg cgtactgccg agcgttcgat ttcattcttg
     6361 tcgataacag ttaggatacg acccatttcg ggataaagtt ttgttcgggt ggcattgatt
     6421 tcaacgtctg aaagttgtac cgacaaggtg tcggtggtaa cttgtcccga aaaacgaaaa
     6481 acattgcatc cgcaaataat tcctgtgatt aaaagaattt tcttcatttg aaaaaatttt
     6541 agttaaacaa tatttatatg aattcgatat aagaatattt tataactaat tataaataag
     6601 ccattaaaat tgaggaaaaa agtatttacc tgttgttatg gttagttgtt cttaaagtaa
     6661 taagaatcag ccttatcaat tctcaacttt ttatatgatt aatacaggta atagccgtat
     6721 cgtacttacg taataattat gctttgattc attttttttc cccgatttgt ttaatatggc
     6781 ctactaacaa aaaaaatcga ctgaaagaaa tgaattcctt tcagtcgaca acaacatata
     6841 taagtacatg aaagacagca ttttacgtct tcagcagaga atataacagg agtattgagt
     6901 tgaatatttt caagaatttc ctccgctcgg aatttttctt ccttgcactg aactaaagaa
     6961 ttaatggatg tagtat
//
LOCUS       DAOTAX010000083         6902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9331_length_6902_cov_27.352125, whole genome shotgun sequence.
ACCESSION   DAOTAX010000083 DAOTAX010000000
VERSION     DAOTAX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_6902_cov_27.352125"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLM04_07655"
     CDS             <1..203
                     /locus_tag="PLM04_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02984.1"
                     /translation="GLMENTHIKITDLNGNLVYETTSNGSLATWNGKNLHGQKVSTGI
                     YLVIGASKDGTQSTITKILVIN"
     gene            474..1418
                     /locus_tag="PLM04_07660"
     CDS             474..1418
                     /locus_tag="PLM04_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02985.1"
                     /translation="MLVLTGIFIVFIQNKINPVDINVDRWSAINNFILNLFQGISPYS
                     AQTHLGGYGSPFPVWQIFHIPFFLLGDIALAMVFCFLCFIITIKWFTDSNKVALSSLF
                     MLTLSPAFWYEVLVRSDLMYNFLCVLIALLIIRKLNINIQSNTVRLGIICGLFLSTRL
                     SIFIPFALYFFYEFKSINFKKQIIFLFTILLVFLLTFLPFAIWDFKTLFLSRHNPFVL
                     QTSQSYGLEIIIVLFLIIYFSLRAKSNFDLTVSYISISFFILVGLTFLIRAFDNKFIW
                     SIFSSAYDISYFNMALPFTIFSLSLFYYQKTENNNSLN"
     gene            1430..1672
                     /locus_tag="PLM04_07665"
     CDS             1430..1672
                     /locus_tag="PLM04_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02986.1"
                     /translation="MNFLEVWLHAGDSEPSHRPACIVWCSEKFEKKYPDKVEALRMNK
                     NKYYRSNFVFHSALDAANIQSKVMDKKLSIFNLPAQ"
     gene            1672..2829
                     /locus_tag="PLM04_07670"
     CDS             1672..2829
                     /locus_tag="PLM04_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02987.1"
                     /translation="MEKELIHKQSSLSFLLLNKYVWAACIVLGLIVKAYFLPVKTGDY
                     VVYLKPWIDFIKTHGYFSALKYDFSNYTPAYLYFLTLVAKIPIEPVFAIKIFSIAFEF
                     LAAYFLGKIVCLKYHNSLIILISLAIIPWLPTVMLNSSYLSQCDAIYASFVMGSIFYA
                     LKKRPFLSVLFLGIAFAFKMQAVFVLPFFFVFLLRKEIKWYYFFIVPAVYLVSILPAW
                     FNGRSFVELISLYFSQADYYRLLTMNFPNTYIWFSGADFDTFSLAGILFTVLLTLVAG
                     FWLRTPKITFTLETWIKFLFLSFIVVPFVLPGMHERYMYLGDIFGLAYLMFFPKKWYL
                     PFGILLVSTYSYIRCSRFSEVLPMQPAFFLYLLVILLTFYDFVESLKFSRK"
     gene            2826..4286
                     /locus_tag="PLM04_07675"
     CDS             2826..4286
                     /locus_tag="PLM04_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON02988.1"
                     /translation="MNAVHKVNFTFLGVIIFVYVLLTAGCLNNCYFWDVVQQVSKEAH
                     WYYQTNFKYLIIPSQNSLSDIVATGYHPPLMGMLTAALWKIFGVHLWISHLLTLICFF
                     VLLYQTLKLVEKFFPAHQAGWVMLVVFLEASLLAQFSIASPDFILLTAFIASLRGLLE
                     RNTWLSSIALIFLFGINMRGIFAGIALWMSFQLFLYLNSEKKFSFTLLAASIRPFLPA
                     IILSLIYFIFYFRMNGWFSGVSEEGGHYSLPENISMIGKHFFEFLLRSVENGRIIVWV
                     FSLFAFYWIAKKKVPISNELKMLICCFLMLFSIYFLFVFITRMPFSARYFMPHFFLLT
                     LITLSVFSFRLPETKWKATVLVFLLFELTGHLWIYPEKMAKSWDCTLAHLPYYELRKQ
                     CFDYIDRQNMDYERISAGFCLYGDRGFTELNQAGKKVGTQLDNQYFIYSNISNLPDEW
                     IDELHNPAHWKPLRTFEKWPVHITVFENISFKQTEK"
     gene            4283..4678
                     /locus_tag="PLM04_07680"
     CDS             4283..4678
                     /locus_tag="PLM04_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HON02989.1"
                     /translation="MNYPKTLVQFLKFGIVGISNTLLTAVTIWLLLRVFHSSNYLANI
                     VGYVVGLVNSFIWNRRWTFENHAQIGATIFKFIVTFAVSYLLQLGFLWLLLHQTEIDD
                     YFCQLLSIVVYTVVNFFMNKYYTFKTEIK"
     gene            4675..5664
                     /locus_tag="PLM04_07685"
     CDS             4675..5664
                     /locus_tag="PLM04_07685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HON02990.1"
                     /translation="MNSSLSVIVPCFNEEEVISESYHRLKKVLQELPLASQIIFVNDG
                     SIDGTEKILSEIAQADKTVKVISFSRNFGHQNAVTAGLNHCLSDLAVVIDADLQDPPE
                     LIPAMIETLEKNNAQVVYCVRKHRKGEGFFKKISAKLFYRFLNFFSEVRLPVDTGDFR
                     LVDKNVIREFNKLHEKGKYIRGLIVWLGFKQVPFYYEREPRFAGETKYPFSKMMKLAT
                     TSLLYFSKKPLKIATYLGFFTVIIALLLALWAIFGKFFGFTNADPGWTSLMVAVVFFG
                     GVQLLTVGILGAYIGNLFDEIKDRPEYVIKDMMNFETGNSTQIADEQTGLQNG"
     gene            5674..6840
                     /locus_tag="PLM04_07690"
     CDS             5674..6840
                     /locus_tag="PLM04_07690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON02991.1"
                     /translation="MIKTLFISAWYPNRYDEMFGLFVRKHAEAVSLYCDVQVLYVHPS
                     SDIRHFETEIRQNGKVKETIVYFPTSKSGVFSKLLKQINFLRAYLKGWRMLQKSGFRP
                     DIVHANILTRTGLVAYGIHLFTRIPYVITEHWTRYLPLRKSYNGLLRKKITEFVVKHA
                     AAVFPVSENLKKAMISNKLYNKNYFVVNNTVDDVFLTKVPKVSETKKIILHVSCFDEQ
                     QKNVKGLLNSLKALSINRSDFKMIFIGTGVDFKEVVDYSVKLNFPEDVIHFVGEKNPA
                     EVAEYMKNADFFVLFSNYENSPVVISESLVCGKPVISTDVGGIAEHINETNGILIPAR
                     DEKMLLESLNFMLDHFQDYDAEKLSQEAVEKFSMKNVGKTIVEQYEKILGKNSE"
BASE COUNT         1917 a         1105 c         1294 g         2586 t
ORIGIN      
        1 cgggattaat ggagaatacc catataaaaa ttaccgattt gaatgggaat ctggtctacg
       61 aaacgacatc gaacggcagc ttggctacat ggaacggaaa aaatcttcat ggacagaaag
      121 tcagtaccgg catttacttg gtgattgggg ccagtaaaga tggtacccaa agtaccatta
      181 cgaaaatatt ggttatcaat taaatttaat aaatttccaa ttatagtggt tgtagcttta
      241 tttaaagtaa ttattttatg attaaaaatt tgagattaaa caaatatttg atattcaatt
      301 tttttatata tattttcatt aattctttat tcctttttaa atatacaact cgaatagatt
      361 ttttatctcc ggaaattttt tttgttgcct atattttttt tataactgtt ttttttcttc
      421 tttgtaaatt tattaatgtt cgttctaact ttcaaaaagt tcttttagtt tttttgctgg
      481 tacttaccgg aatttttata gtttttattc aaaataaaat taatcctgtc gatatcaatg
      541 ttgacagatg gtctgcaata aataatttta ttttaaattt atttcaagga atttctcctt
      601 attcggcaca aacccattta ggtgggtatg ggtctccttt ccctgtttgg caaatttttc
      661 atataccttt ttttttattg ggggatattg cacttgcaat ggttttttgc ttcctatgtt
      721 tcatcataac tatcaaatgg tttacagatt caaataaagt tgcattatcg tcgcttttta
      781 tgttaacact ttctcccgcc ttttggtacg aagttcttgt aagaagtgat ttaatgtata
      841 acttcttatg tgtgttaatt gctcttttaa tcataagaaa attaaatatc aatattcaat
      901 ctaatactgt tagattggga attatttgtg gcttgttttt atctacacgg ttgtcaatat
      961 ttattccttt tgctttgtat tttttttatg agtttaaatc aataaatttt aagaaacaga
     1021 ttattttttt gtttaccatc ctcttggtct ttctacttac ctttttgcct tttgccatat
     1081 gggatttcaa aaccttgttc ttgagcagac ataatccttt tgttttacaa acaagtcaaa
     1141 gttatggttt agaaattatt attgtacttt ttctaattat ttatttttca cttagagcca
     1201 aaagcaattt tgatttaact gtttcataca tctcaatatc attttttatt ttagtaggat
     1261 taaccttttt aattcgagct tttgacaata aatttatttg gagcatattt agttcagcat
     1321 atgatatttc ttattttaac atggcattac cttttactat ttttagtttg agtttgtttt
     1381 attatcagaa aaccgaaaac aataactcct taaattaatt gttgttttca tgaatttttt
     1441 agaagtttgg cttcatgccg gcgatagtga accttctcat cgtccggcct gtattgtttg
     1501 gtgttccgaa aaatttgaaa aaaaatatcc cgataaggtg gaagcgttga gaatgaacaa
     1561 aaacaaatat tatcgcagta attttgtttt tcacagtgct ttggatgcag cgaatattca
     1621 aagtaaagtg atggataaaa agcttagtat tttcaatttg ccggctcaat aatggaaaag
     1681 gaattaattc ataaacaatc atcgttgtct ttcttattgc tgaacaaata tgtttgggca
     1741 gcatgtattg tgctggggtt aattgtgaag gcgtatttct tacccgtaaa aaccggcgat
     1801 tacgtggtgt atctcaaacc ctggattgat ttcataaaaa ctcatggtta tttttcagct
     1861 ttaaaatatg atttttccaa ttatacgccg gcttatcttt attttctgac gcttgttgcc
     1921 aaaatcccaa ttgaacctgt ttttgccatt aaaatttttt ccatagcttt tgaatttctg
     1981 gctgcttatt ttttgggaaa aattgtttgt ttgaaatatc acaattcttt gattatcttg
     2041 atttcattgg ctattatccc ctggttacca accgtgatgc tcaacagtag ttatttgtct
     2101 caatgtgatg ccatttatgc ttcttttgtg atgggcagca tcttttatgc tttaaagaag
     2161 cgaccatttt tatcggtgct ttttctggga atagcttttg catttaaaat gcaggctgtt
     2221 tttgtattgc ctttcttctt tgtttttctt ttgagaaaag aaattaaatg gtattatttt
     2281 tttattgttc ctgccgttta tttggtaagc attttaccgg catggttcaa tggccgaagt
     2341 tttgtcgaat taatttctct gtatttttct caagccgatt actatcggtt gttgaccatg
     2401 aattttccta atacttatat ctggttttca ggagcagatt ttgatacttt ttcgttagcc
     2461 ggcatattat ttaccgtttt gctgactttg gtggcaggct tttggctgcg cactccaaaa
     2521 ataactttca ccctcgaaac atggataaaa tttctatttc taagtttcat tgtggttccg
     2581 tttgttttgc ccggtatgca cgagcgctac atgtatctgg gagatatttt cgggttggcg
     2641 tatttgatgt ttttccccaa aaaatggtac ttgccgttcg gaattttatt ggtttccact
     2701 tattcgtata tccgatgttc gcgctttagt gaagttttac ccatgcaacc ggcttttttc
     2761 ttatatttgt tggtgatttt acttactttt tacgactttg ttgaaagttt aaaattttcc
     2821 cgaaaatgaa tgctgttcat aaagttaact ttactttttt aggcgtcatc atttttgttt
     2881 atgtcttatt gacagccgga tgtttgaata attgttattt ttgggatgtt gtccagcagg
     2941 tttcaaagga agcccattgg tattatcaaa cgaatttcaa atacttaatc attccttccc
     3001 aaaattcttt gtccgatatt gtagcaacag gttatcatcc gcctttgatg ggaatgttga
     3061 cggcagcgtt gtggaaaatt tttggtgtgc atctttggat ttctcatttg ctgacattga
     3121 tttgtttttt tgtgttgctg tatcagacat taaagctggt cgaaaagttt ttccctgctc
     3181 atcaggctgg atgggtcatg ctggtcgttt ttctggaggc ttcacttttg gctcaatttt
     3241 ccatagcttc acccgatttt attttgttaa ctgctttcat agcttcgctc aggggattat
     3301 tagagcgaaa tacatggctt tcgtccattg ccttgatttt tcttttcgga atcaacatgc
     3361 gcggaatttt tgcaggtatt gccttatgga tgagttttca gctttttctt tatctcaatt
     3421 cggagaaaaa attcagtttc acattgttgg ctgcatcgat tcggccattt ttgccggcta
     3481 ttattttatc actgatctat tttatttttt actttcggat gaatggctgg ttttccggag
     3541 tttctgaaga gggaggacat tattcgttgc ccgaaaatat ttctatgatt gggaaacatt
     3601 tttttgaatt tttgcttcgt tcggttgaaa acggtcgcat catcgtgtgg gtgttttcgc
     3661 tattcgcttt ttattggatt gccaaaaaga aagttccaat ttccaacgaa ttgaaaatgt
     3721 tgatttgctg ttttctgatg ctcttcagta tctactttct gttcgttttt atcaccagaa
     3781 tgcctttttc ggcaagatat ttcatgcctc attttttcct gttgacgctg attacccttt
     3841 cggtattttc tttcaggttg ccagaaacca aatggaaagc aacagtactt gtttttcttt
     3901 tgtttgaatt gaccggccat ttatggattt atcccgaaaa aatggcaaaa tcgtgggatt
     3961 gtacgttggc acatttaccc tattacgaat taagaaagca gtgttttgat tacatcgacc
     4021 ggcaaaacat ggattatgag cgcatttcgg cgggtttttg tctgtatggc gacagaggct
     4081 ttacagaact gaatcaggca gggaaaaaag tcggtacaca gctcgacaat cagtatttta
     4141 tttattccaa tatttccaat ttgcccgatg aatggataga tgaattgcat aatccggctc
     4201 attggaagcc tttacgtact tttgaaaaat ggccggttca cattactgtt tttgaaaata
     4261 tttctttcaa acaaacagaa aaatgaatta tcccaaaaca ttggttcagt ttctgaaatt
     4321 cggaatagtc ggaatttcca atacgttgct taccgcagta acgatatggc tgttgttgcg
     4381 ggttttccat tcttccaatt atttggccaa tattgttggt tacgtggttg ggttggtaaa
     4441 cagctttatt tggaatcgac gctggacttt tgaaaatcat gcgcaaattg gcgcaaccat
     4501 tttcaaattt attgttacat ttgctgtttc ctatttgctt caattgggat ttttatggct
     4561 tttgctgcat caaaccgaaa tcgatgatta tttttgtcaa ttattgtcga ttgtggttta
     4621 tacggttgtc aattttttca tgaacaaata ttatactttt aaaaccgaaa taaaatgaat
     4681 tcatccttga gtgttattgt tccgtgtttc aacgaggaag aggtaataag cgaatcatat
     4741 catcggttga aaaaggtttt acaggaactg cctctggctt cacaaataat ttttgtgaat
     4801 gatggaagta tagatggaac ggaaaaaatt ttatcggaaa ttgcccaagc cgacaaaacg
     4861 gttaaagtca tcagtttttc cagaaatttc ggtcatcaaa atgccgttac ggcaggttta
     4921 aatcattgtt tgagcgattt ggccgtagtt attgatgccg atttgcagga tcctcccgaa
     4981 ttgattccgg ccatgattga gacgctggag aaaaataatg cccaagtggt ttattgtgta
     5041 agaaaacatc gaaagggaga aggttttttt aaaaaaatct ctgcaaaatt gttctatcgt
     5101 tttctgaact ttttttccga agtcagactt ccggtcgata cgggcgattt cagactggtt
     5161 gacaaaaatg tgatcagaga attcaataag ttgcacgaaa aaggaaaata tattcgcgga
     5221 ctgatcgttt ggttgggctt taaacaggtg ccgttttact acgagcgcga accccgtttt
     5281 gcgggtgaaa ccaaatatcc tttttccaaa atgatgaagc tggcaactac ttccttgctt
     5341 tatttttcta aaaagccttt gaaaattgca acctatttgg gctttttcac ggtaattatt
     5401 gctttgctgc tggcattatg ggcaattttc ggaaaattct tcggtttcac caacgccgat
     5461 ccgggttgga cgtcactgat ggttgcggtg gtattttttg gaggcgttca attactgacg
     5521 gtcgggattc ttggcgctta tatcggtaat ttgtttgacg aaattaaaga tcggcctgaa
     5581 tatgttatca aggatatgat gaattttgag acaggaaaca gtacacaaat cgctgatgaa
     5641 caaacaggtt tacaaaacgg ataatttgaa aaaatgataa aaacgctttt catatcggca
     5701 tggtatccca atcgatacga cgaaatgttt gggctatttg ttagaaaaca tgccgaagct
     5761 gtgagtttgt attgtgatgt tcaagtactt tatgtgcatc cttcgtccga tattcggcat
     5821 tttgaaaccg aaattcgcca gaatggaaaa gtgaaagaaa ccattgttta ttttccgact
     5881 tccaaatcag gcgtgtttag caaactttta aaacagataa attttttacg ggcatatctc
     5941 aaaggatggc gcatgttgca aaaatccggt ttccgtcccg acattgttca tgccaacata
     6001 cttacgcgta ccggcttggt ggcttacgga atacatttat ttacccgaat tccgtatgta
     6061 attacggaac attggacgag gtatttaccc ttaagaaaaa gttataacgg gttattgaga
     6121 aaaaaaatta ccgaatttgt ggtcaaacat gctgctgcag ttttccccgt gtctgaaaat
     6181 ctaaaaaaag cgatgatcag caataaattg tacaacaaaa attattttgt tgtaaacaat
     6241 actgttgatg atgtatttct gactaaagtt cccaaagtat cggaaacaaa aaaaattatt
     6301 cttcatgttt cctgttttga tgaacagcaa aaaaatgtaa aaggtttgtt gaattcgttg
     6361 aaagctttgt ctataaatcg aagtgatttc aaaatgattt ttatcggaac aggcgttgat
     6421 tttaaggaag tggtagatta ttccgtcaaa ttaaattttc cggaagacgt gattcatttt
     6481 gtgggagaaa aaaatcctgc tgaagttgcc gaatacatga aaaatgccga tttttttgta
     6541 ttgttttcaa attacgaaaa ttcccctgtt gttatttctg aaagtttggt ttgcggtaag
     6601 cccgttataa gtacggatgt tggaggtatt gcagagcata ttaatgaaac aaacggcatc
     6661 ttgattcccg caagggacga aaagatgctt ttagaaagtt tgaatttcat gcttgatcat
     6721 tttcaggatt acgacgctga aaaattgagc caggaagctg tcgaaaaatt cagtatgaaa
     6781 aacgtaggaa aaacgatagt tgaacagtac gaaaaaattt taggaaaaaa ttccgaataa
     6841 taaatttttc aatcaacaag ttttgttgcc tgattaagtt ttttcattgt aaattcattt
     6901 ta
//
LOCUS       DAOTAX010000084         6840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9445_length_6840_cov_30.470597, whole genome shotgun sequence.
ACCESSION   DAOTAX010000084 DAOTAX010000000
VERSION     DAOTAX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9445_length_6840_cov_30.470597"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..3922
                     /gene="purL"
                     /locus_tag="PLM04_07695"
                     /gene_synonym="purI"
     CDS             206..3922
                     /gene="purL"
                     /locus_tag="PLM04_07695"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HON02992.1"
                     /translation="MIYFFQSSEKKIFVVQSAQLLETDISKLKWLFSGAELLDALWVE
                     GNFIGPRREMITPWSTNAVEITQNMGINGIVRIEEFREIPANIPLAEAEFDPMLERSY
                     NGLNQEIFNIDKVPEPIVYIDDIAAYNEKEGLALSKEEIDYLNEVSRKIGRQLTDSEV
                     FGFSQVNSEHCRHKIFNGKFIIDGEEKQLSLFQLIKKTSKENPNKLVSAYKDNVAFIK
                     GPDIEQFAPVKANVPDFFELKKISSVLSLKAETHNFPTTVEPFNGAATGTGGEIRDRL
                     AGGKGSLPLAGTAVYMTSYPRCHEENEKERQWENAVKPRKWLYQSPEQILIKASNGAS
                     DFGNKFGQPLICGSLLTFEHEENGKKYGYDKVIMLAGGVGFANERDALKEAAEPGEKV
                     VLMGGDNYRIGMGGGAVSSVETGQYDNAIELNAVQRANPEMQKRVANVIRALVEAPEN
                     PVVSIHDHGAGGHLNCFSELVENTGGKINIASLPIGDPTLSAKEIIGNESQERMGLLM
                     KEKDIEETKKIAQRERAPFYVAGETTGDMRLVFEQADGQKPLDLKLEYLFGKPPRTVI
                     TDKTLKDEFEPVKFDEEKLQEYLELVLQLESVACKDWLTNKVDRSITGKVARQQCVGE
                     IQLPLSDTGVVALDYRGTSGMATSIGHAPLAALIDPAAGSVLSVAEALTNIVWANLVN
                     GLDSVSLSANWMWPGKNEGEDARLYKAVEACSDFVCSLGINIPTGKDSLSMTQNYGKE
                     KVFAPGTVIISAAGEVADVKKTVSPVLVNDVDSAIYYVDFSFDSFKLGGSALAQVLNK
                     IGTETPTVKDAEYFKTAFDTIQELIEKNLILAGHDISAGGMIVALLEMCFPNTSGGLK
                     IQLDTLVEKSLITILFSENPSVLIQVQNAKTKVVEKILIDNGIGFARIAFPIPENRIE
                     ICKKKLSYCFSIAQLRDVWYRTSYLLDRKQSGKNCALQRYENFKNQPLTFSFPQTFNG
                     KLSQFGLSMHHAKTGAKAAIIREKGTNGDREMAFALYLAGFDVKDVHMTDLVSGRETL
                     EDVNMIVFCGGFSNSDVLGSAKGWAGGFLYNEKAKLALDNFYRRDDTLSLGICNGCQL
                     MIHLGLITPEHEQKPQMLHNDSHKFESGFVSVEIPENHSVMLETLTGSKLGVWIAHGE
                     GKFSLPKAKDDYHIAAQYVYPEYPGNPNGSDYNVAALCSSDGRHLAMMPHPERAIFPW
                     QWAYYPANRKNRDEISPWLEAFVNARKWIEKRKK"
     gene            4916..6616
                     /gene="sulP"
                     /locus_tag="PLM04_07700"
     CDS             4916..6616
                     /gene="sulP"
                     /locus_tag="PLM04_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HON02993.1"
                     /translation="MHFQPKIFHTLKNYKKQQFYADLMAGVIVGIVALPLAIAFGIAS
                     GVTPEKGLFTAIIAGFIISLLGGSKFQIGGPTGAFIVIVYGIVQEFGISGLAIATMIA
                     GVMLICMGIFKLGSIIKFIPFPVVVGFTSGIALTIFTTQIKDLLGLQTGELPGDFIHK
                     WITYGKYIGTINYWALGVSLLSILIIVITPKFSKKIPGSLLAIVIVTLIVYFLREKFG
                     ITGIETIGDRFTINASLPHAETPTISFAVIQSLLPTAFTIAMLGAIESLLSATVADGV
                     IGDSHNSNTELIAQGIANVITPIFGGIPATGAIARTMTNINNGGRTPVAGIIHAIVLL
                     LILLFLGNLTRHIPMSCLAGVLVIVSYNMSEWRTFKALLRNSKSDVAVLVTTFLLTII
                     FDLTIAIEIGLLLAVVLFLRRISETTSISVFKNDINSAEYVEGNADTEKLTLPKGVEV
                     YEIEGPFFFGVASKFEETMKQIGDKPMIRIIRMRKVPFIDSTGLHNLTNLIKLSKKDK
                     IHILLSGVNPQVRESLEKSGIAEMLSEENICENITEAIEKAEKYVAAHEKKKSKKTEA
                     "
BASE COUNT         2228 a         1228 c         1430 g         1954 t
ORIGIN      
        1 aaacacgaat gaatataata ttcagacatg tgggttccat cagaccttta aaaaacaaat
       61 tatattctga tgaaaaagtt tttttgccga aaaacggctt actttgttag acatcgtgat
      121 ttgaattttt agaaatattc tctgaaaatt accgacaatt aaatatttta cgcttaataa
      181 ggaaaacaac taatcctcaa aaaatatgat ttacttcttt caatcttcag aaaaaaaaat
      241 ctttgtcgtt cagtctgctc aattgctgga aaccgatatc tccaagttaa aatggctgtt
      301 ttccggagct gagctgctgg atgccctttg ggtagaagga aattttattg ggccacgccg
      361 tgaaatgata accccttgga gtacaaatgc cgttgaaata actcaaaata tgggaatcaa
      421 cggcattgtt cgtatagaag agttcaggga gattccggct aatattccac ttgccgaagc
      481 ggagtttgat cccatgctcg aacggagcta caatggtttg aatcaggaaa ttttcaacat
      541 cgacaaagtt cctgaaccaa tcgtttacat cgacgatatt gcagcctaca acgaaaagga
      601 aggtttggca ttgagcaagg aagaaattga ttatttgaat gaagtgagtc ggaaaattgg
      661 cagacaactt acggacagtg aagtttttgg attctcgcag gtaaattccg agcattgtcg
      721 ccacaagatt ttcaacggaa aatttattat tgatggagaa gaaaagcaac tatctctatt
      781 tcagctaata aagaaaactt caaaagaaaa tcccaacaaa ctggtttcgg cctacaagga
      841 taacgtagct ttcatcaaag ggcccgacat cgagcagttt gcacctgtca aagcaaatgt
      901 tccggatttt tttgaattga aaaaaatttc atctgttctt tcgctaaaag ccgaaacaca
      961 taattttccc accaccgttg aaccatttaa tggggctgct accggaacag gcggagaaat
     1021 tcgcgaccga ctggcaggag gaaaaggaag tttgccattg gcgggtacgg cagtatatat
     1081 gacaagttac cccagatgcc acgaagaaaa tgaaaaggag cgtcagtggg aaaacgcggt
     1141 aaaacccaga aaatggttgt atcagtctcc ggaacaaata ttaattaaag cttcaaacgg
     1201 agcttccgat ttcggaaata aatttggtca accgcttatt tgtggctctc tgctgacttt
     1261 tgaacacgaa gaaaacggga aaaaatacgg ttacgacaaa gtaatcatgc tggccggagg
     1321 tgtaggtttt gccaatgagc gggatgcact gaaagaagcg gccgaaccgg gagaaaaagt
     1381 tgttttgatg ggtggagaca attatcgcat cggaatgggt ggaggagccg tttcatctgt
     1441 tgaaaccgga caatatgaca atgctataga attgaatgct gttcagcgtg ccaatcccga
     1501 aatgcagaaa agagtcgcca atgttattcg tgctctggtg gaagcgccgg aaaatcctgt
     1561 tgtttcaatt cacgatcatg gcgccggcgg tcatctcaac tgcttttccg aattggttga
     1621 aaataccgga ggaaaaatca atattgcgtc gcttcccata ggcgatccga cactaagtgc
     1681 aaaagaaatt atcggcaacg aaagtcagga acgaatgggt ttattgatga aagaaaaaga
     1741 tattgaagaa acaaaaaaaa tagcccagcg cgaacgtgct ccgttttatg tggccggcga
     1801 aacaaccggc gatatgcgtc tggtttttga gcaagcagac gggcaaaaac cactggattt
     1861 aaaattggaa tatcttttcg gtaaaccacc gcgaaccgtt atcaccgaca aaactttgaa
     1921 agatgaattt gaaccggtaa aatttgatga agaaaaatta caggaatatc tggaacttgt
     1981 tttacaattg gaatcggtag cctgcaaaga ttggctcacc aacaaagtgg atcgttccat
     2041 caccggcaaa gttgcccgtc aacaatgcgt tggagaaatt caacttcctt taagcgatac
     2101 gggagttgta gcattggatt atcgaggcac ttcgggcatg gcaacttcca tcggacatgc
     2161 gcctttggct gcgctgattg atccggcagc aggttcggtg ttgtctgtgg cagaagcctt
     2221 gaccaatatc gtttgggcaa atttggtgaa tggattggac agtgtgtcgt tgagtgcaaa
     2281 ctggatgtgg ccggggaaaa acgagggaga agatgcacga ctttataaag ctgtagaagc
     2341 ttgcagtgat tttgtctgtt cgttgggtat caatatcccg acagggaagg atagcctttc
     2401 catgactcaa aattacggaa aagaaaaagt ttttgcaccc ggaacagtca ttatttcggc
     2461 tgccggcgaa gttgccgatg ttaaaaaaac cgtttcgcct gtattggtaa acgacgtcga
     2521 ttcggctatt tattacgtcg atttttcttt cgacagcttt aaattgggtg gctcggcact
     2581 ggcacaggtt ttaaacaaaa ttggcaccga aacgcccacc gtcaaagatg cagagtattt
     2641 taaaacggca ttcgatacca tacaggaatt gatcgaaaaa aatttaattt tggccggaca
     2701 cgatatttct gccggcggaa tgatagttgc tttattggaa atgtgtttcc ccaatacaag
     2761 cggaggcttg aaaattcagt tggatacgct ggttgaaaaa tcgttgataa ccattctttt
     2821 ttcggaaaat ccaagcgttt tgattcaggt ccaaaatgcc aaaaccaaag tagttgaaaa
     2881 aattcttata gacaacggaa tcggttttgc acgcatagca tttcccatac cggaaaaccg
     2941 aatagaaatt tgcaagaaaa aattatccta ctgcttttct attgcccaat tgcgagatgt
     3001 ttggtatcgt acttcctact tgctcgaccg caaacaaagt ggcaaaaact gtgctttgca
     3061 acggtatgaa aattttaaaa atcagccatt gactttttct tttcctcaaa ctttcaacgg
     3121 aaaactttcg cagttcggct tgtccatgca tcatgcaaaa accggtgcaa aagctgccat
     3181 aattcgggaa aaaggcacga atggcgatcg tgagatggct tttgcgctat atctggccgg
     3241 atttgatgtc aaagatgtgc acatgaccga tttggtttcc gggcgcgaaa cattggaaga
     3301 cgtcaatatg attgtatttt gcggaggatt ttccaattcc gatgtgttgg gctctgcaaa
     3361 aggttgggca ggcggatttc tgtataacga gaaagccaaa ttggcgctgg ataattttta
     3421 tcgaagagac gatacattga gtctcggtat ttgcaatggt tgccagctga tgatacattt
     3481 gggactgata acgcctgaac atgaacaaaa gccacaaatg ctgcacaacg attcccataa
     3541 atttgagtcg ggttttgtaa gtgtagaaat tccagaaaat cattcagtca tgttggaaac
     3601 attgaccgga agcaagctgg gagtttggat cgcacacgga gaaggcaaat tttcattgcc
     3661 aaaagcaaaa gatgattacc acattgcagc acaatatgtt tatcccgaat atcccggtaa
     3721 tccgaatggt tcagattata acgtggccgc tctttgttct tcggatggac gacatttagc
     3781 catgatgcca cacccggaac gggcaatctt tccctggcaa tgggcttatt atcccgccaa
     3841 cagaaaaaac agagacgaaa tttcgccctg gctggaagct tttgtcaatg ccagaaaatg
     3901 gattgaaaaa agaaagaaat aaatttgaac tcgttcggta taaaaatttg acccccatcg
     3961 aaaaaattat tattttaacg tgagttcata aaataagctt attatgttga tgaaaagatg
     4021 atattcacat gttgctaagg ttagttatct ctaaataaag tctttatatt caagaaacga
     4081 accatatttt taaataaaac cttagtaacg ataatcacaa aaacaaaaat aaaccttatt
     4141 accttattaa ttatcaaatt tttatatgaa taaaacatgt aatcgttata tcgagctcac
     4201 gttattttac ggaaatactc gagtggagta cagaaagcaa aaaaccaatg acaagtttat
     4261 cgaatgacca atttttttat tccttttttc agtacaggag aaaaattgag acatttgtat
     4321 atctgtaaaa caatacttat aagccgatct cttcgggtcg accttgtcca gtgagccgaa
     4381 aaaaagtgag aaaagccttt ataaatcttt ttagtttgag ttccgacgta cttcgtcgga
     4441 atgagtttgg gcgatttcag cttttcgagc ctaagttttt cgagtgaatt ggacacacta
     4501 ttgaaagtag cgtttaagtg aaaacaaaat caaatttatt tgatttatgt tgagcgtagc
     4561 tgcttcaact aagtaaattt ttttgcttac ttatttgttt caagacaaaa aagtaagtgg
     4621 ggtttgaggc aaagccccgt tattaaatat ctgaagataa ataatataaa tatttgtaca
     4681 attttttctc ttgtactaag tgaatctata cataaatttt tgctgataaa atttgaatga
     4741 caaaattaca tttaaatttg aaattaaaag gataattgac tcaattgttc tgaagattaa
     4801 agtcattaaa ggcaacgatt cagtttgttt ttatgctaaa aaacatattt agaaggataa
     4861 agttcgttga aatgcagtaa ctttgcactt caaattaaaa aaaaaatttc atgttatgca
     4921 ttttcagcct aaaatttttc atacgctgaa gaattacaaa aagcaacagt tttacgctga
     4981 cctcatggca ggtgtaatag taggaatcgt ggccctgccc cttgctattg cttttggtat
     5041 agcatcggga gttacacccg aaaagggttt gtttactgcc ataattgcag gttttattat
     5101 ttcgcttcta ggaggcagca aatttcagat tggcggccct accggtgcat ttattgttat
     5161 agtgtatggc attgtgcagg aatttggcat atccggactt gccattgcta ccatgattgc
     5221 cggtgtgatg cttatatgta tgggtatttt caagcttggc agtattataa aattcatacc
     5281 atttcctgta gtagtgggtt ttaccagtgg tatagcgctc accattttca ccactcaaat
     5341 caaagatttg ctcggacttc aaaccggtga acttccgggc gattttattc acaaatggat
     5401 tacctacgga aaatatattg gtactattaa ctactgggcg ctcggggtga gtttgctgtc
     5461 aatactcatt attgtgataa caccaaaatt ttcaaaaaaa attcccggat cactgctcgc
     5521 aatcgtcatt gttacactga ttgtttattt tctcagggag aaatttggca taaccggtat
     5581 cgaaactatt ggagatcgtt ttactattaa tgcatcactg cctcatgctg aaactccaac
     5641 aataagtttt gcagtaatac aatcgttatt gcccactgca tttacaattg ccatgcttgg
     5701 tgccatcgaa tcattgcttt ctgctacagt tgctgatgga gttatcggcg atagtcataa
     5761 ctcaaacaca gaattgatag cacagggaat agcaaatgtt ataactccca ttttcggcgg
     5821 aataccggct accggagcca tagcaagaac aatgacaaat attaataacg gtggacgaac
     5881 tccggtggca ggaatcatac atgccattgt gttgttgctc atattacttt ttttaggcaa
     5941 tctcacccgt catataccca tgtcttgtct ggcaggagta ctcgttattg tttcgtacaa
     6001 catgagcgaa tggcgcactt ttaaagcatt gctccgaaac tcaaaatcag atgtagctgt
     6061 attggttacc acttttttac ttacaataat tttcgatctg accatagcca tcgaaatcgg
     6121 gttgttattg gctgtagtac tttttttgcg tcgtatttct gaaactactt ccatatcggt
     6181 tttcaaaaat gacatcaatt cagccgaata tgtagaaggg aatgctgata cagaaaaact
     6241 aactttacca aaaggtgtgg aagtgtatga aattgaggga ccgttctttt ttggagttgc
     6301 aagtaaattc gaagagacca tgaagcaaat tggggataaa ccaatgataa gaataatacg
     6361 tatgcgaaag gtgccattta ttgattctac aggcttgcat aaccttacta acctgataaa
     6421 actttcaaaa aaagataaaa ttcatatcct tctttcaggt gtaaatcctc aagtgcgtga
     6481 gtcacttgaa aaatcgggta ttgctgaaat gctgtctgaa gaaaacattt gtgaaaatat
     6541 cacagaagca atagaaaagg ctgaaaaata cgtggctgcc catgaaaaaa agaaatcaaa
     6601 aaaaacagaa gcgtaagcag actttgcatt caaaaaataa aaatatagtt ataacagata
     6661 aaaaatttac taaagtttcg cagttcagaa agtaatgaaa ataaagggaa aagaagagaa
     6721 aaaatgaata aaaagcagcg tattttatgt aatacatcat taaaaatgag tatattagat
     6781 gttaattaca aaaacagaaa aatatgctac cggacaaaat taaggaaaaa atttctgaaa
//
LOCUS       DAOTAX010000085         6716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9675_length_6716_cov_33.345594, whole genome shotgun sequence.
ACCESSION   DAOTAX010000085 DAOTAX010000000
VERSION     DAOTAX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9675_length_6716_cov_33.345594"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLM04_07705"
     CDS             <1..645
                     /locus_tag="PLM04_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HON02994.1"
                     /translation="MFGIGFYLWRNDNSPQTVKISGFEMGFYGFMTAVVILLLQSRIG
                     MIMFPFGIFLTLLYSLRKKKKLVLSVIVFSAFLVLSAGFYIYKNYRGYFYDRPREIQT
                     DIVLSFLKDHYLLGTGTGGMKVLIPWFPTAHNQFLGDLFHLGIPGLLLLLSLMGSSFY
                     FACKDKNFLLLYFLLIYFVMMQIEMPLSLQKGITYFTLFTGLLLKPRPFGKETK"
     gene            complement(647..1288)
                     /locus_tag="PLM04_07710"
     CDS             complement(647..1288)
                     /locus_tag="PLM04_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HON02995.1"
                     /translation="MSKNKFFVVWKGKQPGIYSSWEECKRQVHQFEGALFKGFATEEE
                     AKKAFESLPWKYIDIRNNAQNKSSGKKGKKTSIGQPIMESISVDAACSGNPGLLEYRG
                     VYTKTGKEIFHQGPFRNGTNNIGEFLAIVHALALMKQNNSQLPLYSDSRTALKWVQQK
                     KAKTKLEKNEENKSLFELIERAENWLQNNDYSTPLLKWETEAWGEIPADFGRK"
     gene            1387..1458
                     /locus_tag="PLM04_07715"
     tRNA            1387..1458
                     /locus_tag="PLM04_07715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Arg,seq:cct)
     gene            complement(1421..2131)
                     /locus_tag="PLM04_07720"
     CDS             complement(1421..2131)
                     /locus_tag="PLM04_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HON02996.1"
                     /translation="MIEASNIHKSFGNLEVLKGVDLSVDKGEVVAIVGPSGAGKTTLL
                     QILGTIDKPNDGVVSINGVTFSKLSEKELSAFRNRNIGFVFQFHQLLPEFTALENVMF
                     PALIAGKDVKKTTNKAKKILEELQLADRMQHKPNELSGGEKQRVAVARALINDPLLIL
                     ADEPSGSLDSKNKTELHKLLFQLRDKLGLTIVIVTHDMELALQTDRVIELKDGMVLKT
                     IEKEKFVVSPGIEPESKV"
     gene            complement(2213..3574)
                     /locus_tag="PLM04_07725"
     CDS             complement(2213..3574)
                     /locus_tag="PLM04_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HON02997.1"
                     /translation="MAKKERINFSKIGAIAAATGSAVGLGNIWRFPYLLGESGGGAFL
                     LVYILCVVFLGLPVMISEFSIGRMAQANAAGSFRTLAPKSKWWLVGLMGVISAFLILG
                     FYVVVSGWTLEYIVQALSNKFANASTAQLNQVFTDFSTNTYRPVGWMLLFTALTCFII
                     IAGVKNGIEKSTKLMMPLLFLIIIILGIRSITLPTGTEGLKFLFKADFGKINSEVILS
                     AMGQAFFSLSLGMGCLITYGSYINKDNNLSSTALQVTIFDTLVAVLASIAIFPAVFSF
                     GINPAQGPELVFITLPNIFSRLHGGYIWAVMFFVLLALASLTSTISLLEVIVAFLTEE
                     LHISRAKAAIVSSLGIALLGILSSFSLGIWKDFSLFGYGFFALFDSITSKILMPVGGI
                     FISLFVGWRIDKQKILLELSSNGKYKIPFLNIFLLITRYLAPLAILLVLLNQLGLTKW
                     LGF"
     gene            3689..4309
                     /locus_tag="PLM04_07730"
     CDS             3689..4309
                     /locus_tag="PLM04_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HON02998.1"
                     /translation="MSLKIQKNINIYLKVEKLVFATNNLHKLQEVKAILDGKVQIQSL
                     SEIGCLDEIPETADTLEGNALLKAKFVYDRFQLDCFADDTGLEIEALGGEPGVFSARY
                     AGLEHDFYKNRMKVLRLMEQQTNRKAQFRTVIALVLQGKEYLFEGKVEGIITREPRGE
                     GGFGYDSIFVPNGYEQTFAELSAEQKNQISHRAHAVKKFADFIVSR"
     gene            4522..>6716
                     /locus_tag="PLM04_07735"
     CDS             4522..>6716
                     /locus_tag="PLM04_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HON02999.1"
                     /translation="MKNKIFYISVFCSFFACFVQADNIGMGKWRMHLAYNSVGQIAQS
                     DKKIYAVSDGALFSVDKLDGSMEFYSKISGLHDTSVSRIEYDQNTRQLLVIYANGNID
                     FINEGGVINLPDLYNKQMTFSKDVQHITFFGNRAYLSCHFGVVALNMVKKEIAETYFI
                     GADAAAVDVLCTAVHGQTIYALSKSAVYKADVTNPYLVNYEVWSTVANLPGSGNFQSV
                     VSFANQLFLLRNGKIYQMNAAGQWSPFLSQVTASNLLVSDGSLFILSTNNNIYLLDNQ
                     LVSKTISGLGAVADAEYDSENNTCWFAANGNGIISYRQEGSLLPVVSTYKPSGPAVNI
                     PWQMRFSGQKLFVVPGGRSSAQYNQQGYVMMFENGVWTNIDGKTIETQTGKRVLDFMD
                     IAVDPNDPTHFLVPSYGTGIYEFKNNQFSKWYNFTNSLIETIFPNDPNGSYLYMRMDG
                     GVFDSEGNVWFTNSDVASGIKILKADKQWEKLTFSGVANKPTLGQILIPSNNPNQKWV
                     LSVRSTPGICVFDDNGTIDDQRDDKSVFFPYFRNTDEAASNISPTYYNCIVEDKNHVI
                     WVGTDMGPLLFYNPSNAFDADFTCSRVKIPRNDGTNLADYLLADETILAIAVDGANRK
                     WIGTKSSGVYLMSENGQETLQHFTVSNSPLLSNNIMSIAINPVSGEVYFGTGKGIVSY
                     QSDAAEAGSSFGDVHAYPNPVRETYNGVITITGLMENTHIKITDLNGNLV"
BASE COUNT         1987 a         1232 c         1375 g         2122 t
ORIGIN      
        1 atgtttggaa tcgggtttta tttgtggcga aacgacaatt caccgcaaac agttaaaata
       61 tccggtttcg aaatgggttt ttatgggttc atgacagccg tagtcatttt attgttgcaa
      121 agtcgtattg gaatgattat gtttccgttt ggcatttttt taactttgct ttacagtctg
      181 cgaaaaaaga aaaagcttgt tctttcagta attgtttttt cagctttttt agttttgtca
      241 gccggatttt atatttataa aaattatcgc ggttattttt acgaccgtcc tcgcgaaatt
      301 caaaccgaca ttgtactttc gtttttaaaa gatcattatt tgttgggtac cggtaccggt
      361 ggaatgaaag tcctaattcc ttggttccca acagctcaca atcaattttt aggagacttg
      421 tttcatttgg gcattcccgg cttgctcttg ttactgtctt taatgggaag ttcgttttat
      481 tttgcctgca aggataaaaa ttttttgctg ctctattttc tgttgattta ttttgtaatg
      541 atgcaaatag aaatgccgct ttccttgcaa aaaggaataa cctattttac tttattcacc
      601 ggattattgc tcaaacctcg accttttgga aaggagacaa agtaagttat tttctcccga
      661 aatcggccgg aatttcgccc caagcttcag tttcccattt cagcagcgga gtagaataat
      721 cattgttttg cagccagttt tctgcacgtt ctatcagttc aaacaaagat ttgttttctt
      781 cattcttttc aagtttggtc tttgcctttt tttgctgtac ccatttcagt gcggtgcggc
      841 tgtcggagta gagtggcaac tgactgttgt tttgtttcat tagagccagt gcgtggacaa
      901 tagccaaaaa ttcgccaata ttgtttgttc cgttccgaaa tggtccctga tgaaaaattt
      961 ccttgcctgt tttggtataa actccgcgat attcgagcag tcccggattg ccgctgcagg
     1021 ctgcatctac cgaaatactt tccattatcg gttgcccgat agatgttttc ttccctttct
     1081 tccctgaaga tttgttttgc gcattatttc gtatgtcgat atatttccaa ggaagggatt
     1141 cgaatgcttt ttttgcttct tcttcagtag caaaaccttt gaatagggcg ccttcaaatt
     1201 ggtgaacttg ccgtttgcat tcttcccatg aagaataaat accgggctgt tttcctttcc
     1261 agacaacaaa aaatttgttt ttgctcatac ggttgatagt tttcaggatg tgaaagatag
     1321 agaaggggaa agtaaatttt tatttatcgc cgaaaaaatg taattttgca ctactttcaa
     1381 aaaaatggtc ttgtagttca atggatagaa cgaaagtttc ctaaacttta gattcgggtt
     1441 cgattcccgg cgagactaca aatttttctt tttctatagt tttcagtacc attccatcct
     1501 tcagctctat taccctgtcg gtttgtaaag ccagttccat atcgtgtgta acaatgacaa
     1561 tggtcaatcc taatttgtcg cgtagttgaa ataataattt gtgaagttcc gttttgtttt
     1621 tggagtcgag gctacctgaa ggttcatcgg ccagaatcag caagggatca ttgattaaag
     1681 cgcgggcaac ggcaacgcgt tgtttttcgc cacccgacaa ttcgttaggc ttgtgttgca
     1741 ttcgatctgc aagctgaagt tcttccaaaa ttttttttgc tttattggtg gtttttttta
     1801 cgtcttttcc ggcaattaag gcgggaaaca ttacattttc caaagccgtg aattcgggaa
     1861 gaagttgatg aaactgaaaa acaaaaccga tatttcgatt gcgaaaagcc gatagttcct
     1921 tttctgataa tttactgaaa gttaccccgt tgatggatac cacgccgtcg tttggtttgt
     1981 cgatagttcc caaaatttgc aacagtgtgg tttttcccgc accacttggt cccactatgg
     2041 ccaccacttc tcctttgtca accgataaat ctacgccttt cagcacttcc aagttaccaa
     2101 aacttttgtg tatgttgctg gcttcaatca ttttttcgtg cgttttgatg ctcacacggt
     2161 cattttcaaa tgtattcatt gttattttta ttcggaataa tgtctcaatt ttttagaacc
     2221 ccaaccattt ggtcaatcct aactgattca gtagaacgag tagaatggca agtggtgcaa
     2281 ggtatcgggt tatcaacaaa aaaatattca gaaaaggtat cttgtatttt ccgttgctgc
     2341 tgagttcgag taaaattttc tgcttgtcga ttctccagcc tacaaataat gaaatgaaaa
     2401 tacctcccac cggcatgaga attttggaag taatactgtc gaacagagca aagaaaccat
     2461 atccaaacaa tgaaaaatct ttccatatcc ctaacgaaaa agatgaaagt atgcccaata
     2521 atgcaatgcc taaggaagaa acaattgcag ctttcgctcg tgatatatgt aattcttctg
     2581 tgagaaaagc aacaattact tccagcagcg agatggtgga agttaaagag gctaaagcca
     2641 gtaaaacaaa gaacataact gcccagatgt aacctccgtg caaccgtgaa aaaatattgg
     2701 gcagcgttat aaataccagt tcggggcctt gcgctggatt gataccaaaa gaaaaaactg
     2761 ccgggaaaat ggcaatggaa gcaaggacgg ctaccagcgt gtcgaaaatg gtaacttgaa
     2821 gcgctgtaga actaaggtta ttgtctttat ttatgtaaga cccgtaagta atcaagcaac
     2881 ccattcccaa actcaaagaa aagaaagcct gtcccattgc acttaaaatg acctcagaat
     2941 tgatttttcc gaaatcggcc ttaaaaagaa atttcaatcc ttctgtgccg gttggtaaag
     3001 taatggaacg aattcccaaa atgatgataa tcagaaacag caatggcatc atcaatttgg
     3061 tgcttttttc aattccgttt tttacaccgg caatgataat aaaacacgtt aatgcagtga
     3121 aaagcagcat ccatcccact gggcggtaag tatttgtact gaaatcggta aatacctgat
     3181 taagttgggc agtggatgca ttggcaaatt tgttgctcaa tgcctgtaca atatactcaa
     3241 gagtccatcc cgaaacgaca acataaaaac ccaaaatcaa aaaggccgaa atcacgccca
     3301 taagtcccac cagccaccat ttgctttttg gcgccaatgt gcgaaacgaa cctgcagcat
     3361 ttgcctgcgc cattcggcca atggaaaact cggaaatcat aaccggcagt cccagaaaaa
     3421 caacacaaag aatgtacacc aacaggaaag ctccacctcc ggattctcca agcagataag
     3481 gaaaacgcca aatgtttccc aatcctacgg cagagcctgt ggcagccgct atggcgccaa
     3541 ttttgctaaa attgattctc tccttttttg ccatattttt cttgttttcg atgcatattt
     3601 gttgcaaaag tagagaaaaa aaataaaaat cgaaatgaat gggagaaagc tgacttgata
     3661 attatttttg taaaatagtt atctttgcat gagtttgaaa attcaaaaga atatcaacat
     3721 ttatcttaaa gtggaaaaat tagtttttgc tacaaataat cttcataaat tgcaggaagt
     3781 taaagcaatt ttggatggga aagtacaaat acaaagttta tcggaaatag gttgcttgga
     3841 cgaaatacca gaaaccgccg atacgcttga aggcaatgct ttgttgaagg caaaatttgt
     3901 ttacgacaga tttcagttgg attgttttgc agatgacaca ggcttggaaa ttgaagcatt
     3961 gggaggcgaa cccggcgttt tttcggcaag gtatgcaggg ttggagcatg atttttacaa
     4021 aaaccgaatg aaagtattgc gtttgatgga acaacaaacc aatcgcaaag ctcaattcag
     4081 aactgttatt gcccttgttt tacaggggaa ggaatatctt tttgaaggaa aagtagaagg
     4141 tataattact cgagaaccga gaggtgaagg tggctttgga tacgattcga tatttgttcc
     4201 caatggctat gaacagactt ttgccgagtt gtcggcagag caaaagaatc aaatcagcca
     4261 tcgagcccat gcagtgaaaa aatttgccga ctttatcgtt tcaaggtgaa agtgaaacca
     4321 aagtttttca ttggtttcat ttttcatcaa tcattgacaa aacaaaataa atcaggaagt
     4381 tttcagttta attttatgga ttaatgtttt acattcaaca acttttttga ggataaataa
     4441 atttttgaaa aataaagatt tccaatacag cgtaatttag taatttaata cgctatttcg
     4501 ttaaaatcaa atatttaact tatgaaaaat aagatttttt acatttcggt cttttgtagt
     4561 ttttttgctt gtttcgttca ggcagacaat attggcatgg gaaaatggag aatgcatttg
     4621 gcttacaaca gtgtcggtca gattgcgcaa tccgataaaa aaatatatgc agtaagtgat
     4681 ggtgctttat tttccgtaga taaactcgat ggcagtatgg aattttacag taaaatcagc
     4741 ggattgcacg atacttctgt cagccgaatc gaatatgatc aaaatactcg acaactgctt
     4801 gtaatttatg ccaatggaaa tatagatttt attaatgaag gcggcgttat taatcttccc
     4861 gatttatata ataagcaaat gactttcagc aaagacgttc agcatattac ttttttcgga
     4921 aatcgggctt atttgtcctg tcattttggt gttgtagcat tgaatatggt gaaaaaagaa
     4981 attgccgaaa cctattttat cggtgccgat gcagcagctg tggatgtgct ttgtacggca
     5041 gttcacgggc agactattta tgcgctttcc aaaagtgcgg tttataaggc agatgttacc
     5101 aatccgtatt tggttaatta tgaagtttgg tcgactgttg caaatttgcc gggaagtggt
     5161 aactttcaat ctgtggtatc ttttgccaat cagctttttt tgcttcgtaa cggaaagatt
     5221 tatcaaatga atgcggcggg acaatggtct ccttttcttt cacaggttac agcatcaaat
     5281 ttgctggttt ctgatggaag tctgtttatt ttgagtacca ataataatat ttatttgctt
     5341 gacaaccagt tggtttcaaa aactatttct ggacttggtg cggttgcgga tgctgaatac
     5401 gacagtgaaa acaatacctg ttggtttgcg gccaatggaa atggaattat ttcctatcgt
     5461 caggaagggt cgctgttgcc ggttgtatct acttataagc cttccggccc ggcggtgaat
     5521 ataccctggc aaatgcgatt ttccggacag aaattgtttg tagtgccggg tggaagatct
     5581 tcggctcaat ataatcagca gggttacgtc atgatgtttg aaaatggtgt ttggacaaat
     5641 attgacggaa aaacaattga aacccaaaca ggaaaaaggg tgttggactt tatggacatt
     5701 gcggttgatc cgaatgatcc tactcatttt ttggttcctt cttatggcac gggtatttat
     5761 gaatttaaaa ataatcagtt cagtaaatgg tataatttca ctaacagcct cattgaaact
     5821 atttttccga atgatccgaa tggttcttat ctttacatgc gaatggatgg cggtgttttt
     5881 gattcggaag ggaatgtgtg gtttacaaac tcagatgttg caagtggaat caagatttta
     5941 aaagccgata aacaatggga gaaattgaca ttttcaggtg tagcaaataa acccacctta
     6001 ggtcagatac ttattccttc taataatccc aatcaaaaat gggtactttc tgtaagatct
     6061 acaccgggca tctgtgtttt cgacgacaat ggaactattg acgatcaaag agatgataaa
     6121 tcggtatttt ttccctattt ccgaaatacg gatgaagcag caagcaatat ttcaccaact
     6181 tactacaatt gtatagtgga ggataaaaat catgtgattt gggtgggaac agatatgggg
     6241 cccttgctgt tttataatcc ttccaatgct tttgatgccg attttacctg ttcgcgcgtt
     6301 aagattccga gaaatgatgg taccaacctg gcagattatt tgctggccga cgaaacaatt
     6361 ctggctattg ctgtagatgg agccaaccga aaatggattg gcacaaaatc atccggcgta
     6421 tatttaatgt cggagaacgg gcaggaaacc cttcaacatt ttacagtttc caacagtcct
     6481 ttgctttcca acaatattat gtctattgcc ataaatcctg taagtggtga ggtctatttt
     6541 ggaacaggga aaggaatcgt ttcatatcaa agcgatgcag cagaagccgg aagcagtttt
     6601 ggcgatgtac acgcctatcc caatccggta agagaaactt acaatggagt catcaccatc
     6661 acgggattaa tggagaatac ccatataaaa attaccgatt tgaatgggaa tctggt
//
LOCUS       DAOTAX010000086         6668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9760_length_6668_cov_30.866173, whole genome shotgun sequence.
ACCESSION   DAOTAX010000086 DAOTAX010000000
VERSION     DAOTAX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9760_length_6668_cov_30.866173"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1109
                     /locus_tag="PLM04_07740"
     CDS             30..1109
                     /locus_tag="PLM04_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HON03000.1"
                     /translation="MSLQLRKNAKYALLTPTSMGVRITPVNGQPVHSSQLFEMQATSA
                     ETNVASISSFLGLPVKVLTTFVKDSPIASFIKSDLHRRNIDFEGPEIPQGGPWGYRHQ
                     FNIADSGYGLRGPRVLNDRAGEVGRTLNVKDFDLERIFSKEGVKIIHLSGLIAALSPE
                     TSNFCLEIARYAKKEGTLISFDLNYRATFWKGREAELRTTFSEIASLSDILIGNEEDF
                     QLCLGIKGPAEGGKGLENAIDSFKEMIMNAQVTFPNVSVFGNTLREVISANEHLWGAI
                     VYNGKDWQVIKPRPIYVLDRIGGGDGFVGGLLYGILKEWNIEKCTQFAWASGALATTF
                     LTDYAQPADEEQIWSIWEGNARVKR"
     gene            1180..2634
                     /gene="gnd"
                     /locus_tag="PLM04_07745"
     CDS             1180..2634
                     /gene="gnd"
                     /locus_tag="PLM04_07745"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HON03001.1"
                     /translation="MKKLADIGLIGLAVMGENLVLNMESKGFTVAVYNRTVEKVDKFI
                     NGRGAGKNFIGAHSLKELVDSLERPRKVMMLVKAGQPVDDLIEQLIPLLDKGDIIIDG
                     GNSHFPDTIRRTKYVESKGLLYIGTGVSGGEEGALKGPSMMPGGSPAAWPYVKDIFQA
                     VAAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQVINEAYFLMKELLGMIDDEQH
                     EVFKKWNTGKLDSYLIEITRDILAFKDEDGTPLVEKILDTAGQKGTGKWTVDAALDLG
                     IPLTLIGESVFSRCLSAQKDLRVEASKAISGPKKAFKGDRQQFINDLENAIYASKIVS
                     YAQGYDLMMQAAKEYGWNLNYGGIALMWRGGCIIRSVFLGDIKKAFDQNPNLRHLLLD
                     PFFKEQVESAQEGWRRVCAVAMENGIPVPAMASALSYFDGFRTERLPANLLQAQRDYF
                     GAHTYERIDRPRGEFFHTNWTGRGGDTASTTYNV"
     gene            2749..4035
                     /locus_tag="PLM04_07750"
     CDS             2749..4035
                     /locus_tag="PLM04_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HON03002.1"
                     /translation="MIYFEKGSATTDLTPEDLKNGLYEALEKLGKRQKVLAIPPDFTR
                     YPSHAGELTEFAWQYYGEKLTDVLPALGTHTGMTDEQIATMFGQVPKTIFRVHDWRHD
                     VITLGYVPGSYVSEVTEGALSFDWPAQVNKLLVEGNFDLILSIGQVVPHEVVGMANYN
                     KNIFVGTGGQEGINKSHFIGAVYGMEKMMGRANTPVRKVFNYASEHFAAHLPIVYVQT
                     VVALNKETGKLQTYGLFIGDDFEVFDKAAGLSMQVNFEMVEKPLNKVLVWLDPAEFKS
                     TWLGNKAIYRTRMAIADGGELIVLAPALREFGEDKEIDRLIRKYGYFGTPKTLKATNE
                     NEELQQNLSAAAHLIHGSSEGRFTITYCPGKNPENLTKEEIESVGFRWGDIDEVTAKY
                     HVSTLKEGWNVMPDGEEIFYISNPALGLWAHKDRFR"
     gene            4659..5123
                     /locus_tag="PLM04_07755"
     CDS             4659..5123
                     /locus_tag="PLM04_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HON03003.1"
                     /translation="MALPKVFRSGSNRTPSLPSLIDRFFDTDLLDWDAFNFAGENMTL
                     PAVNVKETKDEYCIEVAAPGLNKEDFKVSYDDGMLTISSEKQQEKEEKEGEKVTRREF
                     SYESFQRSFTVPEEVVNADKISAKYENGILYVTLPKHEKAKKEKNVKEIKIS"
     gene            5598..6251
                     /gene="rpe"
                     /locus_tag="PLM04_07760"
     CDS             5598..6251
                     /gene="rpe"
                     /locus_tag="PLM04_07760"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HON03004.1"
                     /translation="MNRKIISPSILAADFSNLQAVCELINQSEAQWIHLDVMDGVFVN
                     NISFGFPVLEAVRKHCRKMMDVHIMIVHPEKYVTRFAKAGAEMLTFHFEAAENPQKVI
                     DQIHEEGMKAGITINPDVPVKVLEPFLNQVDMVLLMSVFAGYGGQPFIEETYERMDEL
                     KKMMELKKATCLLEVDGGVNLENAPKLFSHGANVLVAGSSIFKAENPLEIIQQMKNC"
     gene            6343..>6668
                     /locus_tag="PLM04_07765"
     CDS             6343..>6668
                     /locus_tag="PLM04_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03005.1"
                     /translation="MNFLQRTPFIRLLLAYICGIVVYHYAELFPATLWSLFFLSIALL
                     LIPFFIRETRLQFNFRWLFGTGVFLFLFITGYIFSFERDKASKFYPLNQKGIYVVELT
                     SAPVEKA"
BASE COUNT         2017 a         1221 c         1509 g         1921 t
ORIGIN      
        1 cattaattta ataaaaaaat aatattgata tgtcgcttca attacggaaa aatgcaaaat
       61 atgccctgct gacacctacg agcatgggtg tacgtattac tcctgtgaat ggacaacctg
      121 ttcatagcag tcaattgttc gaaatgcagg ctaccagtgc cgaaaccaat gtggcaagta
      181 tttcctcatt tttaggtctt ccggtcaaag tgttgactac tttcgtaaag gacagtccga
      241 ttgcttcgtt tatcaagtcc gacttgcatc gtcggaacat cgattttgaa ggaccggaaa
      301 tccctcaagg tggtccttgg ggctatcgtc atcagttcaa cattgccgac agcggttatg
      361 gattgcgagg gccgcgtgtg ctcaacgatc gtgccggtga agtgggacga acgttgaacg
      421 tgaaggattt tgatctggaa cgaatttttt ccaaagaagg agtgaaaata attcatttgt
      481 cgggattgat tgctgcgctt tcgccggaaa cgagcaattt ttgtcttgaa attgcacgct
      541 atgcaaaaaa agaaggaacg ctcatttctt ttgatttgaa ttatcgggct actttttgga
      601 aaggacgcga agccgaatta aggacaactt tctccgaaat tgcttctttg tcggatattt
      661 taatcgggaa tgaagaagat ttccaacttt gtctgggcat aaaaggaccg gccgaaggag
      721 gcaaagggtt ggaaaatgcc attgattctt ttaaggaaat gattatgaat gcacaggtaa
      781 catttccgaa tgtcagcgtt tttggaaata cgcttcgtga agtcatcagt gcaaatgaac
      841 atttgtgggg tgctattgtt tataatggaa aagattggca ggtcatcaaa ccacgaccca
      901 tttacgtact cgaccggatt ggcggtggtg atggattcgt aggcggttta ctttacggga
      961 ttctgaaaga gtggaatatc gaaaaatgta cccaatttgc gtgggcaagc ggtgcattgg
     1021 caaccacttt ccttactgac tatgctcaac cggccgatga agaacaaatt tggagtattt
     1081 gggaaggaaa tgcccgcgta aaaagataaa agctatagaa attcgacaga agaaacctct
     1141 ttttataaaa cagaatattg aaataaaaac agaaaaaata tgaaaaaatt ggcagatatt
     1201 ggcttgatag gattggccgt catgggtgag aatttggtac tcaacatgga aagtaaaggc
     1261 ttcactgtag ccgtttataa tcgtacggtc gaaaaggtgg ataaatttat caacggacgt
     1321 ggagcaggga aaaattttat cggcgctcat tcgttaaaag aattggtcga ttcgctggaa
     1381 aggccgcgta aagtaatgat gctggtgaaa gccggacaac cggtagatga tttgattgaa
     1441 cagcttattc cgctgctcga taaaggcgat attatcattg atggcggaaa cagtcatttc
     1501 cctgatacca tacgtcgtac taagtatgtc gaaagcaaag gcttgcttta tattggtacc
     1561 ggcgtttccg gaggcgaaga aggagcatta aaaggtccat ccatgatgcc cggaggttct
     1621 cctgcagctt ggccttatgt gaaagatatt tttcaggctg tggccgcaaa agtggaagat
     1681 ggaacgccat gctgcgattg ggttggcgaa aatggtgccg gacattttgt aaaaatggtt
     1741 cacaacggca ttgaatatgg cgacatgcaa gtaatcaacg aagcttactt tttaatgaaa
     1801 gaattgcttg gcatgatcga tgacgagcaa catgaagttt tcaaaaaatg gaacaccggc
     1861 aagttggatt cctatctgat tgaaataacg agagacattc tcgcatttaa agatgaagac
     1921 ggaacgccgc tggtggaaaa aatcctcgac acggccggcc aaaaaggaac cgggaaatgg
     1981 acagtggatg cagcgctcga tttgggcatt ccgttgactc tgattggtga atcggttttt
     2041 tctcgttgct tgtcggcaca gaaagattta cgcgtggaag cttcaaaagc catttcagga
     2101 ccgaagaaag catttaaagg cgaccgccag caatttatca acgatctgga gaatgccatt
     2161 tatgcttcca aaatagtttc gtatgcacaa ggatacgatt tgatgatgca agctgccaaa
     2221 gagtacggct ggaatctgaa ttacggcggc attgcactga tgtggagagg aggctgtatt
     2281 attcgttccg tatttttggg agatatcaaa aaagcatttg accagaatcc gaatctacgt
     2341 catttgctgc tcgatccatt tttcaaagaa caggttgaat cggctcagga aggttggcga
     2401 agagtttgtg ccgttgcaat ggaaaacgga attccggttc cggctatggc ttctgctctt
     2461 tcctatttcg acggattccg caccgaacga ctaccggcaa atcttttaca ggcacaacgc
     2521 gattatttcg gagcacatac ctacgaaaga attgaccgtc cgagaggtga atttttccac
     2581 accaactgga caggaagagg cggcgacact gcttcgacta catacaatgt ttaatgaata
     2641 atgttttttg aatttgatcc attctgacag atgccttcca aaagctctgt cagaattgtt
     2701 tttacagtat gtttctacat cccagaattt tcattatcaa caaccattat gatttatttt
     2761 gaaaaaggtt cggcaacgac cgaccttaca cctgaagatt tgaaaaacgg actttacgaa
     2821 gctttggaaa agttaggcaa gcgacaaaag gtgctggcca ttccgcccga tttcactcgc
     2881 tatccaagtc atgccggaga gttgacggaa tttgcatggc agtattacgg cgaaaagctc
     2941 acggacgttt taccggcatt gggcacgcat accggcatga cggacgaaca aatcgcgacc
     3001 atgttcggtc aagtgccgaa aaccattttt cgtgttcacg actggcggca tgatgtcata
     3061 acgctgggtt atgtgcccgg atcgtatgtt tcggaagtaa ccgaaggagc gttaagtttc
     3121 gactggccgg cccaggtgaa taagctgctg gtggaaggta atttcgattt gattctttcc
     3181 atcggacagg ttgtgccgca cgaagtggtg gggatggcca attacaacaa aaacatcttt
     3241 gtgggaacag gcggacagga gggaatcaac aaaagtcatt ttatcggagc ggtttacggc
     3301 atggaaaaaa tgatgggacg agccaatacg cccgttcgga aagtattcaa ctatgcttcg
     3361 gagcattttg cagcacattt gccgattgta tatgtgcaaa cggtggtcgc tttgaacaaa
     3421 gaaaccggga aactgcagac ttacgggctt ttcattggcg acgatttcga ggtttttgac
     3481 aaggcagcgg gtttatccat gcaagtaaac tttgaaatgg tggaaaaacc cttaaataaa
     3541 gttctggttt ggctcgatcc ggctgaattc aagagcacat ggctcgggaa taaggcgatt
     3601 taccgaaccc gcatggctat tgccgacgga ggtgaactga ttgtgctggc accggcattg
     3661 agggaatttg gcgaagacaa ggaaattgac cgactgattc gaaaatacgg ttattttgga
     3721 acacccaaaa cattgaaagc aaccaatgaa aatgaagaat tgcaacaaaa cctgagtgcg
     3781 gctgcccatt taatacatgg ttcttctgaa ggacgattta ccattactta ttgtccgggc
     3841 aaaaatccag aaaatctaac caaagaagaa atagaaagcg ttggttttcg ctggggtgat
     3901 attgacgaag ttacagcgaa atatcacgtt tctactctga aagaaggctg gaacgtgatg
     3961 cccgacggag aagaaatttt ctacatttcc aatcctgctt tgggactttg ggctcacaaa
     4021 gacaggtttc ggtaattcgt ctgtttgaat tttcagcgtt tctgctcgag agagttttct
     4081 ttaaaattat tacaggaaat cggtcatagg aaaatgattg gatttcgttg atcgacgtaa
     4141 caaggcgaac ttgaaatcac agaaaattca tttacaaaat tgcttttacg tactttattt
     4201 tatctatccg caacctttta ctcagatgtt aaagaaagct tcaaaaaagt cgtcagtcca
     4261 tataaaaatt tgtagaaggt tgtatttcaa tttgaatggt agaaaaaata tttgacaatt
     4321 tttccttgtt ggataatttc taaagtgtaa ttggttttat taattgaaaa ccttctcaaa
     4381 ctaaaaatac tcagcgaata atttagtcct gtcatattgg cagtttttta tacatttcga
     4441 cagatattat tcaattatta cgctttaaag gtgctttgtt tttttaaatc cataaattct
     4501 attggtcaat tagctctgtt tttcaatggt atagatatga tgtcaataat ttttattttt
     4561 gctggtacgc gttttgaaca ataaaatatg aaatttatca aaaaagattt caaaaagcaa
     4621 ttgatggttt aacttataaa atataggagg tgttaattat ggcattacca aaagttttca
     4681 gatctggaag taatcgaact ccttcacttc catcgttgat tgacagattt tttgatactg
     4741 acttactgga ttgggacgca ttcaactttg caggtgaaaa tatgacgctg cctgcagtga
     4801 atgtaaaaga aacaaaagat gaatattgca tagaggttgc tgctcccggt ttgaacaaag
     4861 aagatttcaa agtaagttat gacgacggga tgttgaccat atcttccgaa aaacagcagg
     4921 aaaaagaaga aaaagaagga gaaaaggtta ctcgtcgcga gttcagttat gaatcgttcc
     4981 agcgtagttt taccgttcct gaagaagttg tgaatgcgga caagatttct gcaaaatatg
     5041 aaaacggtat tttgtatgta accttgccaa aacatgaaaa agctaagaaa gaaaaaaatg
     5101 taaaggaaat caagatttca taattttcta ctttcaatcc aataaaaaca ggaggatttt
     5161 caggaaagga agatcttcct gtttttttga caacttaaaa aaattcagtt agaaccttac
     5221 aaaaaaatta aattcattga agaagttccg ctcaacataa atcaaataaa tttgattttg
     5281 ttttcgctta agcgctgctt tcaagactgc tcccgcttca ctcgaaaaac ttacgctcga
     5341 aaagctgaaa tcgcccaaac tcattctgac gaaatacgtc ggaacccaaa caaggacgat
     5401 ttttaaacgc ttttctcact tatttttcgg ctcaccggac aaagtaaacc ccaagtgttt
     5461 ggcttatatg tcctgtttta cagatatata aaacaaatgt tacaattttt gtcatgtgct
     5521 aaacttgtta aattacattt tttgttattt ttgcaaaagc acagctgtta attttctaaa
     5581 tttataaggg acatcgcatg aacagaaaaa tcatttctcc ttcaattttg gcggccgact
     5641 tcagcaattt gcaggctgtc tgcgaactga tcaaccaaag tgaagcacaa tggattcact
     5701 tggacgtgat ggacggagtt tttgtcaaca acatttcttt tggttttcct gtattggaag
     5761 ctgtacgcaa acactgtcga aaaatgatgg atgttcatat tatgatcgtt catcccgaaa
     5821 aatacgtaac acgctttgca aaagcaggcg ccgagatgtt gacttttcat tttgaggctg
     5881 ccgaaaatcc acaaaaagta attgaccaaa ttcatgagga agggatgaaa gccggcatca
     5941 ccatcaatcc tgatgtgcct gtcaaggtgc ttgagccttt tctcaatcaa gtggatatgg
     6001 ttttgttgat gagtgttttt gcgggttatg gcggtcagcc gtttattgaa gaaacttacg
     6061 aacgcatgga tgagctgaag aaaatgatgg aactcaaaaa ggctacttgc ttgctggaag
     6121 ttgacggagg tgtgaatctg gaaaatgccc ccaagctttt ctctcatgga gcaaatgtgc
     6181 tggtggcagg aagttcgata ttcaaagccg aaaatccttt ggaaataata cagcagatga
     6241 aaaattgttg aaaagtcttt ctcgcctttg gcggcacgat tttaaaattg aaaaattttc
     6301 cggccggagg gtttcaaata aaacattttt caggtatttt gcatgaactt tttacagcga
     6361 acccctttca ttcggttact gcttgcctac atctgcggca ttgtagtgta ccattatgcg
     6421 gaattgtttc ccgcaacgct atggtctcta ttttttcttt caatcgcatt actgctgatt
     6481 ccttttttta tccgcgaaac gcggcttcaa ttcaatttca gatggctatt cggcactggc
     6541 gtattccttt ttctttttat tacgggttat atttttagtt ttgaacgcga taaagcatcg
     6601 aagttttatc ccctgaacca aaaagggatt tatgtggttg aattgacttc ggctccggta
     6661 gaaaaagc
//
LOCUS       DAOTAX010000087         6547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_9999_length_6547_cov_31.609365, whole genome shotgun sequence.
ACCESSION   DAOTAX010000087 DAOTAX010000000
VERSION     DAOTAX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_6547_cov_31.609365"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLM04_07770"
     CDS             <1..294
                     /locus_tag="PLM04_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03006.1"
                     /translation="EVGLFDERFFLYPEDIDLTRRIRRKFRSVFYPHVSVVHYHERGS
                     YKNLRMLWIHIINMIKYFNKWGWFCDAERKRVNRETMKQYFSQCHEIKIKSIN"
     gene            558..1307
                     /locus_tag="PLM04_07775"
     CDS             558..1307
                     /locus_tag="PLM04_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HON03007.1"
                     /translation="MTSWTQRTELLIGKSGMEALQQASVLVVGLGGVGGMAAEMICRA
                     GIGKMTIVDHDTINPTNLNRQILASVNTISQYKTDVMAQRLLQINPDLKLKTFNQWAE
                     VSNIEELLETEKYDFVVDAIDTVTPKVFLIKSCLERRIDIVSSMGSGGRMDPSKVRIT
                     DISNTHTCPLAKAVRQRLSKMGITKGLAVVFSDELPNKNAVLPVEDEKFKKSTVGTIS
                     YMPAIFGCMMASHVIREILRKNHFQKTLDRI"
     gene            1331..4174
                     /locus_tag="PLM04_07780"
     CDS             1331..4174
                     /locus_tag="PLM04_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HON03008.1"
                     /translation="MDERDDREEITGAENEEDFVHQADIEDNSETKPSGHSSYKIPKI
                     ENSEVKHHLSGMYQNWFLDYASYVILERAVPDIYDGLKPVQRRILHSMRRLEDGRYNK
                     VANIVGHTMQFHPHGDASIGDALIQLGQKDLLIDCQGNWGNILTGDSAAAPRYIEARL
                     SKFALETVFNPKTTEWKLSYDGRNKEPVNLPVKFPLLLAQGVEGIAVGLNSKILPHNF
                     NELIDACIAYLKGEDFELYPDFQTGGLIDVSHYNDGERGGVVKIRAKINKVDNKTLSI
                     TEIPYGTNTSKLIDSILKANDKGKIKIRKVDDNTASSVEILVHLQPGTSPDKTIDALY
                     AFTDCEISISPNCCVIHDRKPRFMNVSEMLRVSCDHTKDLLEAELNIQKQELLEQLFF
                     TSLEKIFIENRIYKDKGFEDSSSQDAVVNYIDQRLEPFKENFIRAVTREDILRLLDIK
                     MVRITKFDSDKANDTLIAIQKKIDEVDFHLNHLIDYTISWYQGLKDRYGKNYPRRTEI
                     RNFETIIATKVAEANEKLYVNREEGFIGTSLKKDEFVCNCSDIDDIIVFFKDGRYKVT
                     KVAEKMFVGKNILYVNVFKKNDKRTIYNVVYRDGKAGPYYIKRFAVNGITRDKEYDLT
                     QGSAGSKILYFSANPNAEAEVIRVTLKPKPRMTKLVFEKDFGEIAIKGRQAMGNILTK
                     NEIHKITLKQKGGSTLGGRQVWFDHDVLRLNYDKHGEYLGEFQSDDLILIVSKNGEYY
                     TTNFDAANHYENNILIIEKFDENKVWSAVVYDGELKNYYLKRFQLTPSQKKNSFVGEH
                     PESRLILLSSVDFPRFEVVFGGHDARRNPLIVDAKEFISVKSYKAKGKRLTTFEVETV
                     NELEPVRFKEKNNEESTVINSDEEEDNENASENDRISNSSDAQESPEKATDVERKTNV
                     NSQTKEDAVSNAINEDRPEIHSTDEEGSQLTLF"
     gene            4963..5511
                     /locus_tag="PLM04_07785"
     CDS             4963..5511
                     /locus_tag="PLM04_07785"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HON03009.1"
                     /translation="MKKFIFLFLGFLPVILIFSSCNNTKTYAELLKDEKAIIASYIKR
                     SNIQVVTTFPADGEWDNKYVKTSSGLYFHLVSPGDTAANADTVAKNDRVVIRYRQYTL
                     TEYPDTIYYWNTIDYPYPISFTYLDYTQACTAWHEAVSYMKRNNSEAKLIVPSKIGLS
                     EYINSVTPLGYDFKIVGIQKSY"
     gene            5542..>6547
                     /locus_tag="PLM04_07790"
     CDS             5542..>6547
                     /locus_tag="PLM04_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HON03010.1"
                     /translation="MALIKSISGIRGTIGGRAGEGLTPLDIVRFSAAYAKFMKQNAGN
                     DSNKIVVGRDARISGDMVYHLVAGTLSGQGLEVVNIGLATTPTTELAVIYEKALGGII
                     LTASHNPMQWNALKLLNEKGEFLNAKEGEEVLKIAAEENFDFASVEQLGKISENFTFL
                     QKHIDNILSLNLVEVDAIKKADFTVAIDAVNSVGGIAVPALLEALGVKKVIKLNCEPT
                     GIFAHNPEPLPEHLSEISSLVKESKADVGFVVDPDVDRLAIICEDGSMFGEEYTLVAV
                     ADYVLQHTPGNTVSNLSSSRALRDITQKRGGKYSAAAVGEVNVVTLMKQTNAVIGGEG
                     N"
BASE COUNT         2157 a         1119 c         1369 g         1902 t
ORIGIN      
        1 gaagttggcc tgtttgacga acgttttttt ctgtatcctg aagatatcga tctgacgcgt
       61 agaatacgcc gaaaatttcg gtcggttttt tatccacatg tttcggtggt acattatcac
      121 gaacgcggtt cttataaaaa tttgcggatg ttgtggattc acattatcaa tatgataaaa
      181 tatttcaaca aatggggttg gttttgtgat gccgaacgaa agcgagtaaa tcgggaaact
      241 atgaagcagt atttttcgca atgccatgag attaaaatta aaagtataaa ttaatttatg
      301 gtatctatcg atattttttg gaaaacttaa tttctaaatt caaaaactaa aatttaactc
      361 tgtaaacatg cataaaagaa tgtaacaaat aacatataac tactataacc ttctaaccca
      421 aattctttca atttgaggtt attggtctca aacttgcaac tctactccat tttattcaaa
      481 ccgatcaaaa aatttctctt ccaaaagtat ggatttgtcc tcgcaaaagt ttaactttgt
      541 atgacaatta tacccatatg acttcgtgga cacaacgtac agagttactg attggaaagt
      601 cgggcatgga agccttgcaa caggcatcgg tgctcgtagt gggtttaggc ggtgtgggtg
      661 gcatggctgc agaaatgatt tgccgtgccg gtataggcaa aatgacaatt gtggatcacg
      721 ataccatcaa tcctaccaat ttaaacaggc agatactggc gtctgtaaac acgatttcgc
      781 aatacaaaac cgatgtgatg gcccaaaggt tgttgcaaat caatcctgat ttaaaattaa
      841 aaacttttaa tcaatgggca gaagtatcga atatcgaaga attgcttgaa acagagaaat
      901 acgattttgt agtagatgcc attgatacgg ttacgcctaa agtttttcta ataaaaagct
      961 gtttggaacg gcgtatcgat attgtttcct caatgggttc gggaggcaga atggatcctt
     1021 caaaagtcag aattacggat atttccaata cgcatacttg tccattggca aaagctgtac
     1081 ggcagcggct gtcgaaaatg ggcataacaa aaggccttgc cgttgttttt tcggatgaat
     1141 tgcccaacaa aaatgccgta ttacctgttg aagatgaaaa gtttaaaaaa tctactgtgg
     1201 gaaccatttc gtatatgcct gccatttttg gctgtatgat ggcttctcat gtaattcgtg
     1261 aaattttaag aaaaaatcac ttccagaaaa ctttagatag aatttaggaa aaaatggagt
     1321 aaattaatat atggacgaaa gagatgacag ggaagaaata accggagcgg aaaatgagga
     1381 agatttcgtt catcaggccg atattgaaga caattcagag acgaaacctt ccgggcattc
     1441 ttcttataaa ataccgaaaa tagaaaacag tgaagtaaaa catcatcttt ccggcatgta
     1501 tcagaattgg tttctggatt acgcatccta cgtaattctt gaacgtgccg tacccgatat
     1561 ttacgatgga ctgaaacctg tgcagcgacg tattcttcat tccatgagaa gactcgaaga
     1621 cggtcgttac aacaaggtgg ccaatattgt ggggcatacc atgcaatttc atccgcatgg
     1681 agatgcttcc attggagatg cgcttattca gctgggacag aaagatttgc ttatcgactg
     1741 tcagggaaac tggggaaaca tcctcacggg agactctgca gctgcacctc gttatatcga
     1801 ggcacgtctt tccaaatttg cactcgaaac cgttttcaac cctaaaacaa cggagtggaa
     1861 actatcttac gacggtcgga acaaggaacc ggtgaatttg ccggtgaaat ttccattact
     1921 actggctcag ggagtggaag gaattgcagt tggattgaat tctaaaattt tgcctcacaa
     1981 ttttaacgaa ttgattgatg cttgcattgc ttatctgaaa ggtgaagatt ttgaactgta
     2041 tcccgatttt caaacaggcg gacttatcga cgtcagccat tacaatgacg gcgaacgcgg
     2101 aggagtggtt aaaatcagag ctaaaatcaa taaggttgac aataaaacat tgtctatcac
     2161 cgaaatacct tacgggacaa atacgtcgaa actgattgat tccattttaa aggccaacga
     2221 caagggaaaa ataaaaatca ggaaagttga cgacaatact gcgtccagcg tcgaaatatt
     2281 ggttcatttg caacccggaa cttcacccga caaaaccatt gatgcgctgt atgcctttac
     2341 cgattgtgaa atcagcatat cgcccaactg ctgcgtgatt catgaccgga aaccgcggtt
     2401 tatgaatgtc agcgaaatgc ttcgcgtcag ctgcgatcat accaaagatt tattggaagc
     2461 cgaacttaat attcagaagc aggaactttt ggagcagctt ttttttacat cactggaaaa
     2521 aatttttatt gaaaacagaa tttacaagga taaaggtttt gaagacagct caagtcagga
     2581 tgccgtagtc aattatatcg accaacgttt ggaacccttc aaggaaaatt ttatcagagc
     2641 cgtaacaagg gaagatatat tgagactact cgacattaaa atggttcgca ttaccaaatt
     2701 tgattccgac aaagctaacg atacgctgat agctattcag aaaaaaattg atgaagtgga
     2761 ttttcactta aatcatttaa tcgattatac catctcttgg tatcagggtt taaaggatcg
     2821 ttacgggaaa aattatcccc ggcgcacgga gattcgtaat tttgaaacca ttatcgcaac
     2881 caaagtggcc gaagccaacg aaaaactata tgtaaaccgt gaagaaggtt ttattggaac
     2941 ttcgctgaaa aaggatgaat ttgtttgcaa ttgttcagat attgacgata ttattgtgtt
     3001 tttcaaggat ggaagataca aggttaccaa agttgccgaa aaaatgtttg tagggaaaaa
     3061 tattctctat gttaatgttt tcaaaaaaaa tgacaaacga acgatttata acgtggtgta
     3121 cagagatggg aaagccggac catattacat caaacgtttt gccgtcaacg gcattactcg
     3181 cgacaaggaa tacgatttga cgcagggttc tgccggttca aaaattttgt atttttcagc
     3241 caatcccaat gccgaagccg aagtaattcg tgtaacgttg aaacccaaac cgcgcatgac
     3301 caagctggta tttgaaaaag attttggaga aattgccata aaaggccgcc aggcaatggg
     3361 aaacattctg accaaaaacg aaattcataa aattacgctc aaacagaagg gaggttctac
     3421 gctcggagga cgtcaggttt ggttcgacca tgatgtttta cgattgaatt acgacaaaca
     3481 tggagaatat ttgggagagt ttcaaagcga tgacctgatt cttattgttt caaaaaacgg
     3541 cgaatactac accacgaatt ttgatgctgc caatcattac gaaaacaata ttctgattat
     3601 tgaaaaattt gacgaaaata aggtttggtc ggcagtggta tatgacggag aattaaaaaa
     3661 ttattatctg aaaagatttc aacttactcc atcgcagaaa aaaaacagct ttgttggtga
     3721 acatccagaa tctcgattga ttctgctttc gagtgtcgac tttccaagat ttgaggttgt
     3781 ttttggtgga cacgatgccc gaagaaatcc cttaatagtt gatgctaaag aatttatcag
     3841 tgtaaaaagt tacaaagcca aaggaaaacg tttgacaact tttgaagtag aaactgttaa
     3901 cgaattggag cctgttcggt ttaaagaaaa gaataatgaa gaaagtacgg taatcaacag
     3961 cgatgaggaa gaagataacg aaaacgcttc ggaaaacgat cgaatatcaa attcttctga
     4021 tgcacaagag tcgccggaaa aggcaactga tgttgaaagg aaaaccaacg taaattccca
     4081 aacaaaagaa gatgccgttt caaatgctat aaacgaagac aggccggaga ttcactcaac
     4141 tgacgaagag ggcagtcagt tgaccttgtt ttgatttaat atacagtcaa ccataggcaa
     4201 taacttatgc ttccttattt caaatttttt caaaaacgtt agttttaaga aacccacatg
     4261 tattgcacac acaaacacaa atgaatataa tattcagaca tgtgggttcc atcagacctt
     4321 taaaaaataa attatattct gatgaaaact gctattaaat ttgccataaa aatgagatga
     4381 ataaacagga aagcggtaat agcatataca gtttacaagt tcatattcat aaagtgtgat
     4441 tacaaagatg tttatgcagt atttcaacgg aaaaccactg cgttgttgag atattttacg
     4501 ctaaatatgc aacgggaaca agtagagatt ttcaaagaag tggtgagtaa ggaagatgtt
     4561 tttttaagcc agccatttca aataagttta agcgatataa aaagcgatta aaagggaaaa
     4621 gttcagggat gttgttgcag gaaatttcag gaacggcata gtcggggttg gattaatcat
     4681 ttttatacgc aaggttgtgg atgttcgagt acagacaagg taacggatga gatgataaat
     4741 tcctatttgg atcacaaaaa acaatccaat tctaatgaaa attacatttt taaataagtt
     4801 tatgaacatt aatttaacat tttaattgac ttatagccaa aattaaagcg aattttattt
     4861 gcaatccaac ctatggattt tcaatccata gatttgagtt tcttaacttt ttctttctac
     4921 ttttttatgg tacatttgta taaaattaat ttatacggtt ttatgaaaaa attcattttt
     4981 ttgtttctgg gttttttgcc tgttatccta attttttctt cgtgcaacaa cactaaaact
     5041 tacgccgagc ttttaaagga tgaaaaagcc attatagcaa gttatattaa acgtagtaat
     5101 atacaggtag ttacaacttt tcctgctgat ggagaatggg ataataaata tgtgaaaact
     5161 tcaagtggtc tatattttca tttggtcagt ccgggcgata ctgctgccaa cgccgacacg
     5221 gtagcaaaaa atgatcgggt tgttattcgt tatcgacaat atacactgac cgaatatccc
     5281 gataccattt attactggaa taccattgac tatccttatc cgatttcttt cacttatctt
     5341 gattatacgc aagcctgtac ggcttggcac gaagccgttt cctatatgaa acgcaacaat
     5401 tctgaagcaa aacttattgt tccttcaaaa ataggcttga gcgaatatat aaacagcgta
     5461 actccactgg gatacgattt taagattgtt ggcattcaaa agagctatta attttttaac
     5521 aaacttctaa ttaagtttta tatggcactg ataaaatcta tttccggaat tcgtggaact
     5581 attggtggcc gagcaggaga gggattaaca cctctggata tcgttcggtt ttcggctgct
     5641 tatgccaaat ttatgaaaca aaatgcaggt aatgattcaa ataaaattgt agtgggacgt
     5701 gatgcccgaa tttcgggaga tatggtatat catttggtag ccggtacctt atcgggtcaa
     5761 ggactggagg tggttaatat tggattggcc actacgccta ctacagagtt ggctgtcatt
     5821 tacgagaaag ctttgggtgg cattattctt actgccagcc acaacccaat gcagtggaat
     5881 gctttgaaac tgcttaacga aaaaggtgag ttcctgaatg cgaaggaagg tgaagaagta
     5941 ttgaaaatag cggccgagga aaatttcgat tttgcttctg ttgagcagtt gggaaaaatt
     6001 tccgaaaatt ttactttttt gcagaaacac attgataata ttctgtcgct gaatttagta
     6061 gaagttgatg ctataaaaaa agctgatttt acggttgcca tagatgcagt aaattcggtg
     6121 ggaggaattg cagtaccggc tttgctcgaa gcgttgggcg ttaaaaaagt tattaaactc
     6181 aattgcgaac cgacaggaat ttttgctcac aatcccgaac cgcttcccga acatttgtct
     6241 gaaatctcgt cgttggtaaa agaatctaaa gccgatgtag gttttgtggt tgatcctgat
     6301 gtggatcgtt tagccattat ttgtgaagac ggcagcatgt ttggtgaaga atatacgttg
     6361 gtggctgtgg cagattatgt attgcagcat acaccgggaa ataccgtttc caatcttagt
     6421 tcgtcgagag ctttacgcga tattacacaa aaacggggtg gaaaatattc agctgctgct
     6481 gttggtgaag tgaatgtggt taccttaatg aaacaaacga atgctgtaat tggtggtgaa
     6541 ggtaatg
//
LOCUS       DAOTAX010000088         6512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_10055_length_6512_cov_30.201951, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000088 DAOTAX010000000
VERSION     DAOTAX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10055_length_6512_cov_30.201951"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1241
                     /locus_tag="PLM04_07795"
     CDS             204..1241
                     /locus_tag="PLM04_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON03011.1"
                     /translation="MLKKRSGKKIYDTSYTHFETEGIDYEYLSIKRNIFNVFKERLQR
                     EKTFLEYNELIFNQIYNNVKRENFQLIHAHGMYEPPAGLIAKYFSEKLKIPYIITCHG
                     SDVNYKMPLTPKLYIDTFEQAAKVIFVSNAILNKAKSYGYSGKNAGIIPNGVNIDLFK
                     MLDKEKIKQELHFTKKVVGFVGNLLPVKRADKLPEIFEEINQNFETDFLIIGEGRLKN
                     KIEKNCRKKNLNITFTGKLPHEKISYYLNAMDVLILPSRNEGFPCVVLEAQACGVPVV
                     GSNNGGIPEAIGEGGIVVNEGENFEKRFAEGVIKMLKNPIDKNFLRNRATEYSWNNIT
                     KMEFDIYQNFF"
     gene            complement(1226..2698)
                     /locus_tag="PLM04_07800"
     CDS             complement(1226..2698)
                     /locus_tag="PLM04_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HON03012.1"
                     /translation="MGDNLKQKTLSAFTWTTIDRFGQQLVQLIIGIILARLLSPGDYG
                     LMGMVMIFAALSYVLVESGFGQALIRKTEANETDYNSVFYFNIGIALVLYALLYFLSP
                     AIANFFKQPELVSVNRITFLAIIFNAFYLVPYSKSAKILDYKTIAKVNIISVSLSGLL
                     GLTLAFFHYGIWVLVIQHVSYHFFRMIFFYVFVRWQPKLIFSFRVIRSFWKFSIHLLN
                     TSILNIIFNYLFVIILGKFYKKSEVGYYMQGNKLSETFNFTFQSILLGSTFSLFSHIQ
                     HDDARFRRIFGELSNKISIITFPIILCLIAAGKPLIEIVWSAKFLPSVPYFRLLCLAS
                     LFLPLYTLNISALNARGQSRITFRIEMIKKILILIAIFITFSKGIIPMLWGYAVANIA
                     AYFLSIFYIKSELKHDLKQQILAFIQSLGWGLVLGLMVASLSLLIKPSYFLLPVQIFL
                     AAAVYLLIIKIFYKELYDKGMDFLKNRLLSLKQGLHQKKF"
     gene            complement(2720..3607)
                     /locus_tag="PLM04_07805"
     CDS             complement(2720..3607)
                     /locus_tag="PLM04_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HON03013.1"
                     /translation="MAKFNPFTKKNWIFIRQYILITIGLFMYAAAWKGFLLPHQITGG
                     GVTGIGAIVYYATGVPIFITYFVINALLIAVALRIIGFQFSLRTIYGVAMITLFFAVL
                     PQADPGTFVAPNDNFMACLLGGLISGTGLGIVFLNNGSSGGTDIIAKIVNKYYNITLG
                     RILLYCDVLIICSSYFFEFGTIEKIVYGLTNMAISNLAIDMVINGVRQSVQFFIFSKE
                     YEQIANRINTEVHRGVTILDGTGWYSQQPVKVITVIARKNESSMIFRIVKEIDPDAFV
                     SQSSVIAVYGKGFDVIKSK"
     gene            complement(3648..6404)
                     /gene="leuS"
                     /locus_tag="PLM04_07810"
     CDS             complement(3648..6404)
                     /gene="leuS"
                     /locus_tag="PLM04_07810"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HON03014.1"
                     /translation="MEYNFREIERKWQTYWKDNQIYKTVENPSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGFDAFGLPAEQYAIQTGQHPAITTKN
                     NIARYRQQLDKIGFSYDWDREIRTCDPDYYHWTQWAFIQMFHHYYDNNLQKAQPISRL
                     VEHFEKKGTENLHASGTRELSFTAADWKALSEKQQQEILLNYRIAYLADTKVNWCPAL
                     GTVLANDEVSEGVSIRGGYPVEQRIMRQWCLRVSAYAQRLLDGLETIDWTDSLKETQR
                     NWIGRSEGAEIRFSIKGREETFEVFTTRADTIFGVTFMVLAPESELVKICSTPQQAAE
                     VEKYLENTKKRTERERLADRRITGVFSGSYAIHPVTGKEIPIWISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIIPLIEGCDVSEESFDAKEGIMINSGFLNGLTVKEAIEA
                     TKKYITEKGIGRVKVNYRLRDAIFSRQRYWGEPIPIYYKDGMPYTLSEEELPLELPEV
                     DKFLPTEKGEPPLARAKNWKTKEGYPLEVCTMPGFAGSSAYYLRFMDPKNDQALVSPE
                     ANQYWRNVDLYLGGTEHATGHLIYSRFWNKFLYDLGIVCEEEPFKKLINQGMIQGRSN
                     FVYRIKDTNTFVSYHLKDQYETTPIHVDVNMVSNDVLDLEKFKNWNPEYKNAEFILED
                     GKYICGWAIEKMSKSMFNVVNPDDIVENYGADTLRLYEMFLGPLEQSKPWDTNGIDGV
                     HRFLKKLWNLFYKNDELCVTEEKPTAEELKVLHKTIKKISYDIENFSYNTAVSAFMIC
                     VNELFTLKCNKKAILEPLLILLAPFAPHISEELYHALGNSDTVFNASFPQYEEEFLKE
                     NTVSYPVSFNGKVRFTLEMPTDVSKEEIEKTALSHPQTIKYMEGKTPKKIIIVPGKII
                     NIVF"
BASE COUNT         2100 a         1205 c         1234 g         1973 t
ORIGIN      
        1 agcaaaactc taattttttg agaaattagt ttcaaaaacc caatatattg aataagaaag
       61 tattatattt taccaatcta ttcccttttt tcgatgattt acaaggaggc tctttcctaa
      121 ctaatcgatt aaaggaaatg aaagaaaaaa atctttcttt caaggtttat ggcattattc
      181 ccgatgacac tgttttggtc aagatgctaa aaaaaagaag tggaaaaaaa atttacgata
      241 cttcctatac ccattttgaa acagaaggaa ttgattatga atatctttcc attaaaagaa
      301 atatttttaa cgttttcaaa gaaagattac aaagagaaaa aactttttta gaatataatg
      361 agttgatttt taatcaaata tataataatg ttaaaagaga aaattttcag ttgattcatg
      421 cccacgggat gtacgaacca ccggcaggat taattgcaaa atacttctcg gaaaaactaa
      481 aaatcccata cattattact tgtcatggta gcgatgtcaa ttataaaatg cctttgacac
      541 ctaaacttta tatagatacc tttgaacagg cagcaaaagt tatatttgta agtaatgcca
      601 tattaaataa agccaaaagt tatggttatt ctggaaaaaa tgccggtatt attcctaatg
      661 gggttaatat agatttattt aaaatgctgg ataaagaaaa aattaaacaa gaacttcact
      721 tcacaaagaa agtagttgga tttgtaggaa atctcttgcc ggtaaaaaga gcggataaat
      781 tgccagaaat atttgaggaa ataaatcaaa attttgagac tgattttttg attattggcg
      841 aaggtcgatt gaaaaacaaa attgagaaaa attgtagaaa aaaaaatttg aatatcactt
      901 ttactggaaa attacctcat gaaaaaattt cttattatct gaatgcaatg gatgtgttga
      961 tattacccag cagaaatgaa ggttttcctt gcgtagtgtt ggaagctcag gcttgcggag
     1021 tgcccgtagt tgggagcaac aatggaggaa ttcctgaagc aattggagaa ggtggaatag
     1081 ttgtgaatga aggtgaaaac tttgaaaaac gatttgccga gggagtgata aaaatgttaa
     1141 aaaatccgat agataaaaat tttttaagga acagagctac tgaatactca tggaataata
     1201 ttactaaaat ggaatttgat atatatcaaa attttttctg atgtagccct tgttttaaac
     1261 tgagcagtct attttttaga aaatccatac ctttatcata cagttctttg taaaaaattt
     1321 tgataattaa cagataaaca gctgctgcaa gaaagatttg aacgggcagc aaaaaatagg
     1381 agggttttat cagcaaactt aaggaagcaa ccatcaagcc taaaacaagt ccccaaccca
     1441 gactctgaat aaatgccagt atttgttgtt tcaaatcatg tttcaattct gattttatgt
     1501 agaatatcga aagaaaatag gctgcaatat ttgcaactgc gtagccccag agcatgggaa
     1561 ttattccttt gctgaacgta ataaaaatgg caatcaagat aagtattttt tttatcattt
     1621 ctattctgaa agtgattcgc gactgacctc ttgcattcaa tgcactgata ttgagtgtgt
     1681 aaagtggtaa aaacaaggaa gccagacaaa gaagccggaa ataagggaca gacggaagaa
     1741 attttgccga ccaaacaatt tctatcaatg gttttccggc agcaatcaga cacaaaatta
     1801 tgggaaaagt aataatggag attttgttcg acagctcacc aaaaattctc ctgaaacggg
     1861 catcatcatg ctgaatatga gagaaaaggg aaaatgtact gcccaataaa atggattgaa
     1921 aagtaaaatt gaaagtttca cttaatttgt ttccttgcat gtagtatcca acttccgatt
     1981 ttttgtaaaa tttccccaaa ataataacaa acaggtaatt gaaaataata tttaaaatgg
     2041 aagtattcag taaatgaatg ctaaatttcc agaaactacg aataacccgg aatgaaaaaa
     2101 tcaattttgg ttgccatcgg acaaagacat aaaaaaatat cattctgaaa aaatgatagg
     2161 aaacatgttg aattaccagc acccagatgc cataatgaaa aaaagcaagt gtaaggccca
     2221 gcaaaccgct gagcgaaaca gaaataatat tgactttggc aatggtttta taatcaagaa
     2281 tttttgccga ttttgaatag ggaaccagat aaaaagcatt gaaaataatg gccagaaaag
     2341 taattctatt caccgaaaca agttcgggtt gcttaaaaaa attggcaata gccggcgaaa
     2401 gaaaatacaa aagtgcatac aaaaccaaag caataccgat gttgaaataa aatacactgt
     2461 tgtagtccgt ttcattggct tcggtttttc tgatcaaggc ctgtccaaat ccactttcaa
     2521 cgagcacata cgaaagtgcc gcaaaaatca tgaccatgcc cattaatcca taatcgcccg
     2581 gagaaagaag cctagccagt ataatgccaa taatcaactg aaccagctgt tgaccgaagc
     2641 ggtcaatggt cgtccaagta aaagcgctga gggttttttg tttcaggtta tcgcccatgt
     2701 ggtaatggta caataaaaat tatttgctct tgatcacatc gaagcctttt ccgtaaacgg
     2761 caatcacgga tgattgggat acaaaggcat cgggatcaat ttcttttaca attctgaaaa
     2821 tcatggaaga ttcatttttt cgggcaatga cggtaataac ttttaccggc tgttgcgaat
     2881 accatcccgt cccatccaga atggttacgc cccgatgaac ttccgtattg attcggttgg
     2941 ctatttgttc gtattctttt gaaaaaataa aaaattgaac agactgtcgt actccattga
     3001 taaccatatc gatggccaaa tttgaaatag ccatgttggt caagccgtac actatttttt
     3061 caatggttcc aaattcaaaa aaataggaag agcaaataat cagcacatcg caataaagca
     3121 gtatcctgcc caatgtaatg ttgtagtatt tgttgacgat ttttgcgata atatccgttc
     3181 cgccggaact gccgttattg aggaaaacga tacccaatcc cgttcccgaa atcaaacctc
     3241 ccagcaaaca tgccataaaa ttatcgtttg gagcaacaaa agttcccggg tctgcctgtg
     3301 gaagcacggc aaaaaacagg gtaatcatgg caaccccgta aatggtgcgc aagctgaact
     3361 gaaatccgat aatacgcagt gctacagcta tgagcagggc attaataaca aaataagtga
     3421 taaaaatcgg aacccccgtt gcataataaa ctattgctcc tatacctgta acacctccac
     3481 cggtaatttg atgcggcagc aaaaaacctt tccatgctgc agcatacata aataaaccga
     3541 tagttatcag gatgtattgt cggataaata tccagttttt ttttgtgaaa ggattgaatt
     3601 ttgccattat ttatttggga ttgaatgttt ttcctgaatt tttcttgtca gaaaacaata
     3661 ttaataattt ttcccggaac aataataatt ttcttcggcg tttttccttc catgtatttt
     3721 atggtttgtg gatgtgataa agcagttttt tcaatttctt ctttgctgac atcggtcggc
     3781 atttccaacg taaatcttac ttttccgtta aaagaaacag ggtaagaaac cgtattttct
     3841 ttcaaaaatt cctcttcata ttgtggaaaa gaagcattga aaacagtatc gctattgccc
     3901 aatgcatggt aaagttcttc cgaaatgtga ggggcaaaag gtgccagcag tatcagtaaa
     3961 ggttcaagta tggctttttt attgcacttc agggtaaaca attcgttcac acaaatcatg
     4021 aacgccgaaa cagctgtgtt ataggaaaaa ttttcaatgt cgtagctgat ttttttaatg
     4081 gttttgtgca aaacttttaa ttcttctgca gttggttttt cttctgttac acaaagttca
     4141 tcgtttttgt aaaagagatt ccacaacttt ttcaaaaagc gatgcacgcc gtcaattcca
     4201 ttggtatccc aaggttttga ctgttccagt ggacccaaaa acatttcgta caaccgaagg
     4261 gtgtctgcgc cgtaattttc gacaatatca tcagggttga caacattgaa catcgatttc
     4321 gacatttttt ctatagccca accacaaatg tactttccat cttcaagaat aaattcggca
     4381 tttttgtatt cggggttcca gtttttaaat ttttccagat cgagaacatc gttggaaacc
     4441 atgttgacat caacatgaat cggagttgtt tcgtattggt cttttaaatg ataggaaaca
     4501 aaggtattcg tgtcttttat tcggtacaca aagttgcttc ttccctgaat catgccttga
     4561 ttgatgagtt ttttaaatgg ttcttcttcg caaacgatac ctaaatcgta caaaaatttg
     4621 ttccagaaac ggctgtaaat caaatgaccc gttgcatgtt cggttccacc gagataaaga
     4681 tccacgtttc tccagtattg gttggcttca gggcttacaa gtgcctggtc gtttttcgga
     4741 tccataaatc gtaaataata agctgaagaa cctgcaaaac caggcatagt acaaacttca
     4801 agcggatagc cttctttggt tttccagttt ttagcgcgag ccaatggcgg ttcacctttt
     4861 tcggtaggca aaaatttgtc cacttcgggc aattcaagcg gtagttcttc ttcgctaagt
     4921 gtgtagggca ttccgtcttt gtaataaatg ggaatgggtt cgccccagta acgttggcgg
     4981 ctgaaaatag cgtcgcgtag acgataattg acttttaccc tgcctattcc tttttccgta
     5041 atatattttt ttgtggcttc aatggcttct tttacggtca gtccattcag aaatcccgag
     5101 ttgatcatga taccttcttt ggcatcgaaa ctttcttcac tgacatcaca cccttcgata
     5161 agcggaatga tgggcaaatt gaaatgtttg gcaaaagcgt agtctctgct gtcgtgcgcc
     5221 ggaacagcca taatagcccc tgttccgtaa ccagcaagca cataatcgga aatccaaatg
     5281 ggaatttctt tcccggtaac aggatgaatg gcataagaac cggaaaaaac tcccgtaata
     5341 cgccgatctg ccaagcgttc acgttctgta cgctttttgg tgttctcgag gtatttttca
     5401 acttcagctg cctgttgggg agtggaacat atttttacca attcgctttc aggagcaagt
     5461 accataaatg taacgccaaa tatggtatcc gcacgtgtgg taaatacttc aaaagtttct
     5521 tctctgcctt ttatggaaaa acggatttcg gcaccttcac ttcttcctat ccaattcctt
     5581 tgtgtttctt tcagggagtc ggtccagtcg atagtttcca aaccatccag caaacgttgg
     5641 gcataagccg aaacgcgcag acaccattgc ctcatgatac gttgctctac aggatatcca
     5701 ccgcgaatgg aaacgccttc gctcacttcg tcgttggcca gcaccgtacc caaagccgga
     5761 caccagttca cttttgtgtc ggccaaatag gcaatgcgat agtttaacaa aatttcctgt
     5821 tgttgttttt cgctcaacgc tttccagtcg gcagccgtaa acgacagttc acgagtgccc
     5881 gaagcatgaa gattttcagt accttttttc tcaaaatgtt caacaagacg actaatgggt
     5941 tgagctttct gcaaattgtt gtcataataa tgatgaaaca tttgaatgaa ggcccattgt
     6001 gtccagtggt aataatcggg atcgcaggtt ctgatttctc tatcccaatc gtaactgaat
     6061 ccgattttat caagttgttg gcggtagcga gctatgttgt ttttggtcgt aatggccgga
     6121 tgttggcctg tttgaatggc atattgttct gccggcagtc cgaaagcatc aaagcccatt
     6181 ggatggagta cattaaaacc tttcagtctt ttgtaacggc tgtaaatatc cgatgcaatg
     6241 taaccgagcg gatggccgac atgcagccct gctcccgaag gatagggaaa catatccaat
     6301 acataatatt tgggtttgct tgggttttca acggttttat aaatctgatt gtctttccaa
     6361 tatgtttgcc attttcgctc tatttctctg aaattgtact ccatgtatca ctgaaattta
     6421 ataaattcaa ccttatcttt tctcttttac agtattcgca aattaaattt gcaaagttaa
     6481 tcattttgcc aaaataaaag aaagcttctt ct
//
LOCUS       DAOTAX010000089         6419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_10239_length_6419_cov_33.077938, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000089 DAOTAX010000000
VERSION     DAOTAX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10239_length_6419_cov_33.077938"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..589
                     /locus_tag="PLM04_07815"
     CDS             194..589
                     /locus_tag="PLM04_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03015.1"
                     /translation="MKAFKALKLRWSIEVFFKEAKQLLNPGGCQSNDFDAQIADTTIS
                     MIAYILLSFRYRYEHYESMGELFRVMNAECLQQTLDERLWKLFLDLLREICEAFEKDI
                     DELFELIMNCPKTAELVSRMLAPPLQEAV"
     gene            complement(740..2629)
                     /locus_tag="PLM04_07820"
     CDS             complement(740..2629)
                     /locus_tag="PLM04_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HON03016.1"
                     /translation="MANYLQVENLTKSFGDRLLFENISFTVTENQRVALIAKNGTGKT
                     TLLNILTGGEDYDSGNIVYRSDLRIGYLLQNPDFRKDLTVMDACLTSDSEIVRTIAQY
                     ELCLSSHNEQKMNEIVARMDALRAWDYELRVKQILTKLKITDFDQKIGELSGGQLKRV
                     ALAQVLITEPDLLLLDEPTNHLDLEMVEWLEDFLKRSSMSLLMVTHDRYFLDRICTDI
                     LEIDFQKLFHYKGNYTYYLEKRQERLDAFNAETDRTRQLYRTELEWMHRQPQARGTKA
                     KSRIDRFHEIEQRARQRFDDKVIKLNVKASYLGSKIFEAKNVSKSYGELKILENFNYV
                     FSRYEKLGITGNNGTGKTTFLKMLLGEVQPDSGTFDIGETVVFGYYSQEGLNFDEQMK
                     VIDVVQNIAEVIDLGNGKKMSASQFLQYFLFSPESQYDYVYKLSGGERRRLYLCTILM
                     KNPNFLILDEPTNDLDIVTLNVLEDYLQNFKGCLIVVSHDRYFMDKVVDHLLVFKGNG
                     ILKDFPGNYTDYREWNDLMEEQERENEKKLKQNLFGGLSQKNETNEKNRPKKLTYKEQ
                     KELETLEKEIPILENEKREIEIRLGSGEPNSEEMVQLSHRFSELMQLIDEKSFRWLEL
                     MENID"
     gene            2762..3445
                     /gene="mnmD"
                     /locus_tag="PLM04_07825"
     CDS             2762..3445
                     /gene="mnmD"
                     /locus_tag="PLM04_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HON03017.1"
                     /translation="MDSELILTQDGSHTLFVPDIDECYHSTFGAIGESTLIFIQSGFN
                     CCEKDKINVLEVGFGTGLNAFLTLLEAENRNKKVFYMAFELFPLSVEKAKQLNYPEKV
                     SPEKRFFFEKLHTSIWGEEVPITPFFKLLKINADFIVSSLPSVYDLIYFDAFSPEKQP
                     EMWSQKIFRKIFDHCNENAILTTYCVKGVVRRALQNTGFVVEKLPGPPGKREFLRAVK
                     PSNADFTEK"
     gene            3551..3820
                     /gene="rpsO"
                     /locus_tag="PLM04_07830"
     CDS             3551..3820
                     /gene="rpsO"
                     /locus_tag="PLM04_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HON03018.1"
                     /translation="MYLTQEKKKEIFGQYGKSNTDTGSPEAQIALFSYRINHLTEHLK
                     SNKKDYSTERALIKLVGKRRRLLDYLKEKDINRYREIIKELGIRK"
     gene            3908..5116
                     /locus_tag="PLM04_07835"
     CDS             3908..5116
                     /locus_tag="PLM04_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HON03019.1"
                     /translation="MRYLYSIGIHIYRFLIFLASFFNPKARELYKGQKNALRYLKSNI
                     EENARYVWFHAASLGEFEQGRPIMEALKRQHPDLKILLTFYSPSGYNVRKDYRGADVV
                     AYLPPDTRRSARAFVKTVKPLKAIFIKYEFWPNFLLTLRAEGVPTYAVSAIFRPNQIF
                     FKWYGKWYLQLLTTFRRLFVQDQDSAVLLEKHGIHQVSVSGDTRFDRVFALFSEAKKL
                     PLVEAFVAGKPVIVAGSTWPEDEDLLVKYLKQHPKIKLILVPHEIHKNHLEQISKLLD
                     GKFIRYTEASENNIADYNCLVVDVVGILSSIYQYAKVAYVGGGFGVGIHNILEAAVWN
                     VPVVFGPNFQKFKEARDLIQLKGAFSINDYTSLEENLDKLLCDEKAGQMAGNYVRENI
                     GATELICNNL"
     gene            5213..>6419
                     /gene="hisS"
                     /locus_tag="PLM04_07840"
     CDS             5213..>6419
                     /gene="hisS"
                     /locus_tag="PLM04_07840"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HON03020.1"
                     /translation="MNQKPSIPKGTRDFSPEEMTKRNYIFDTIKSVFRLYGFQQIETP
                     AMENLSTLLGKYGEEGDKLLFKVLNSGDFLSGISDAELLERNAARLSGKICEKGLRYD
                     LTVPFARFVVQNRDKISFPFKRYQIQPVWRADRPQKGRYREFYQCDADIIGSNSLFNE
                     LELIQIIEEVFCRLKINVCIKINNRKILSGIAEYIGEPEKLTDITTAIDKIDKIGIEN
                     VNEELLSKGINREALGKLQPLLQLKGSNQEKLAQLKALLFESAAGMKGITELETIFDL
                     CEKANVCVPIELDLTLARGLNYYTGAILEVKALDVEIGSISGGGRYDNLTGVFGWEGV
                     SGVGVSFGADRIYDVLYQLQLFPEILAEQTKILFVVFGEKELKFSLQWAQALRKENIN
                     TEIYAEPSKL"
BASE COUNT         1945 a         1183 c         1252 g         2039 t
ORIGIN      
        1 acaaaaacag aaaaatatgc taccggacaa aattaaggaa aaaatttctg aaacaggaac
       61 agatttcaat gaaacccaca tgtattacat acacaaacac gaatgaatat aacatttaga
      121 catgtgggtt ccatcagacc attaaaaaat taaattatat tctgatgaaa ataaattaga
      181 gagcatacct attttgaaag ctttcaaggc gttgaaacta aggtggtcga tagaagtatt
      241 tttcaaagaa gcgaagcaat tgttgaatcc gggaggatgt caatccaatg atttcgatgc
      301 acagatagcc gataccacga taagcatgat agcctatatt ttactgtctt ttcgatatcg
      361 gtatgaacat tatgaatcga tgggagaact cttcagggta atgaatgcgg aatgtttgca
      421 gcagactctt gacgaacgtc tttggaaatt atttttggac ttgctgcgtg aaatatgcga
      481 agcatttgag aaagatattg acgaattgtt tgagttaatc atgaattgtc caaaaacggc
      541 agaattagtt tcaaggatgc ttgctcctcc gcttcaggaa gcagtttgac gaaaatggaa
      601 ttattaatat gttgacaatc agacgggaaa tgactaaatc gcacttcttt attttctgtc
      661 tttcaaatca ttaaaacgaa gtgcgaaact ttagcaatat taaattcaat tttgaatttt
      721 tctttttctc gcaatcttat cagtcgatat tttccataag ttcgagccag cggaaagatt
      781 tttcatcgat caattgcatt agttccgaaa atcgatggga taattgtacc atttcttcac
      841 tgttcggttc tcccgatccc agccggattt ctatttctct tttctcattt tctaaaatcg
      901 ggatttcttt ttccaaagtc tccaattctt tttgttcctt gtaggtcaat ttcttaggcc
      961 gatttttttc atttgtttcg tttttttgag acaagccacc gaacaaattt tgctttaact
     1021 ttttctcatt ttctctttcc tgctcttcca tcaaatcgtt ccattcccgg taatcggtat
     1081 aatttcccgg aaaatccttc agaatgccat ttcctttaaa taccagcaaa tgatctacca
     1141 ctttgtccat aaaatagcgg tcgtggctta ccacaatcaa gcagccctta aaattctgca
     1201 aataatcttc caatacattc agcgtaacaa tatcgaggtc gtttgtgggc tcgtccagaa
     1261 ttaaaaaatt gggatttttc atcaagatgg tacagagata caagcgcctt ctttcacctc
     1321 cactaagttt gtaaacataa tcgtactgac tttcgggaga aaagaggaag tactgtaaga
     1381 actgagatgc cgacattttt ttaccgttac ccaaatcaat cacttcggca atattctgaa
     1441 ccacatcaat gactttcatt tgttcgtcaa aattcaatcc ttcctgacta tagtaaccaa
     1501 aaacgacagt ttctccgata tcgaaagttc cactgtcggg ctgaacttct cccagcagca
     1561 ttttcaaaaa agttgttttt cccgttccgt tgtttcctgt aattcccagt ttttcataac
     1621 gggaaaacac ataattgaaa ttctccagta ttttaagttc gccataagat tttgaaacat
     1681 tttttgcttc gaaaattttt gaacccaaat agctggcttt aacattgagt tttattactt
     1741 tgtcatcaaa acgttgacgt gcgcgctgtt caatctcatg aaaacgatcg atgcgcgatt
     1801 ttgcctttgt tccgcgagct tgaggttggc gatgcatcca ttccaattct gttcgataca
     1861 gttgtcgggt tcggtcggtt tcggcattaa aagcatcgag cctttcctgt cgtttttcca
     1921 aatagtaagt ataatttcct ttgtaatgaa agagtttttg aaaatctatt tccaaaatgt
     1981 cggtgcaaat tcggtcgaga aaataacgat cgtgggtaac cataagtagg gacattgaag
     2041 atcgtttcaa aaaatcttcg agccattcta ccatttccaa atccaagtgg ttggtcggtt
     2101 catcgagcaa aagcaagtcc ggctccgtaa tcaatacttg agccagtgcc actcttttaa
     2161 gttgtccacc ggaaagttca ccgatttttt ggtcaaaatc ggttattttc aactttgtaa
     2221 gaatttgttt gacacgcagt tcgtagtccc aagctctcaa agcatccatg cgcgcaacaa
     2281 tctcattcat cttctgctca ttgtgtgaag aaaggcaaag ttcatattgt gctatcgttc
     2341 tcactatttc gctgtcggaa gttaagcagg catccatcac ggttaagtct ttcctaaaat
     2401 ccggattttg aagtaaatat cctatgcgca agtcgcttcg gtaaacaata tttccgctgt
     2461 cgtaatcttc gccaccggta aggatattca ataaggttgt ttttcctgtt ccatttttag
     2521 ctatcagtgc aacccgctga ttctcggtaa cagtaaaaga aatattttca aaaagcagtc
     2581 gatcgccaaa tgatttggtg agattttcca cttgcagata gttcgccata ttcaataatt
     2641 tgatacaaag ataacacttt ccgagcattg ggtcataaat gtttgccatt tcgcgtgttt
     2701 cactcctcgt gaaaaattat taattttact gccaataaat tgtaaattct gttttcaata
     2761 aatggatagc gaactcattt taacacaaga tggttcacat acgttgtttg ttcccgatat
     2821 agatgaatgt tatcattcta ctttcggtgc gataggtgag tccaccctga tttttataca
     2881 aagtggtttc aactgttgtg agaaggataa aatcaatgtg cttgaagttg gttttggcac
     2941 gggtctgaat gcttttctga cattgttgga agcggaaaat cgaaacaaaa aagtttttta
     3001 tatggcgttt gaactttttc cattgtcggt tgagaaagcc aaacaactga attatcccga
     3061 aaaagtttca ccggaaaaac gtttcttttt cgaaaaactg catacttcta tttggggtga
     3121 agaagtaccc attactccct tttttaagtt gttgaagatt aacgctgatt ttatcgtttc
     3181 ctctttgccg tcagtttacg accttatcta ttttgatgct ttttcgccgg agaaacagcc
     3241 tgaaatgtgg tcgcagaaaa tattcaggaa aatttttgac cactgcaacg aaaatgccat
     3301 attgaccacc tattgtgtaa aaggtgtggt cagaagagca ctacaaaaca cgggatttgt
     3361 tgtggaaaaa cttcccgggc cgccggggaa acgtgaattt cttcgggctg tgaagccttc
     3421 aaatgctgac tttacagaaa agtaacgaat taattctcaa ttttttcaaa agaatttgaa
     3481 aaaagagtgt tcgttaatta aaagaaaatt tatacctttg cagttcatta ttaaacataa
     3541 aactttcatt atgtatttaa ctcaagaaaa gaagaaagaa atctttggtc aatacgggaa
     3601 gtctaatact gatactggtt cgcctgaagc ccagatagca ttgttttctt accgtatcaa
     3661 ccatttgact gagcatttga agtcaaacaa aaaagattat agtactgaac gcgctttgat
     3721 taaattggta ggtaaacgtc gtcgcttgtt ggactacctg aaggaaaaag acatcaaccg
     3781 ttaccgcgaa attatcaaag agttgggcat tcgtaagtaa ttcaggcatt tgtcaacttt
     3841 taaaaggcgt ttcaaaacta cttgaaatgc ctttttaatt tagaaacctc tttccgcaaa
     3901 ttaaaggatg cgttatttat attccattgg tatacatatt tacagatttc ttatttttct
     3961 tgcttcattt ttcaatccca aagctcgtga actttacaaa gggcagaaaa atgcgctccg
     4021 atatttaaaa agcaatattg aagaaaatgc ccgttatgtg tggtttcatg ctgcctcttt
     4081 gggcgaattt gagcaaggaa gaccgataat ggaagcattg aaacgccaac atcccgattt
     4141 aaaaattctc ctcacttttt attctccttc aggttataat gtcagaaaag attatcgagg
     4201 tgccgatgtg gtagcatact tgccacctga tacgcgtcgt tcggcacgtg catttgtaaa
     4261 aacggtgaaa ccactgaaag ccatttttat taaatatgaa ttttggccaa attttttgtt
     4321 gacgcttcgg gcagaaggtg ttcccactta tgctgtttcg gcaatttttc gccccaatca
     4381 gattttcttc aaatggtacg gaaaatggta cttgcaattg ttgaccactt tccgacgttt
     4441 gtttgttcag gatcaagatt cagcagtttt acttgaaaaa cacggcattc atcaagtcag
     4501 cgtcagtggt gatacgcgtt tcgatagagt ttttgctctt ttttccgaag caaaaaagct
     4561 tccgttagtt gaagcgtttg ttgctggaaa accggtaatt gtggcaggta gtacttggcc
     4621 agaagacgaa gatttgttgg tgaaatatct taaacaacat ccgaaaatca aattgatatt
     4681 ggttccccat gaaattcata aaaatcattt ggaacaaatc tcaaaacttt tggatggaaa
     4741 attcatacgc tataccgaag catccgagaa taatattgct gattataact gtttggtggt
     4801 cgatgtagtg ggaatacttt cttccattta tcagtatgca aaagtggctt atgtaggcgg
     4861 gggctttggt gtaggtattc acaatattct tgaagcagcg gtttggaatg tgccggttgt
     4921 atttggccca aattttcaaa aattcaagga agcccgcgat ctgattcaac tgaaaggtgc
     4981 tttttccatc aatgattata cttcgctgga agaaaatttg gataagttgc tttgcgacga
     5041 aaaggcagga cagatggcag gcaattatgt tagagaaaat attggcgcta cagaattgat
     5101 ttgtaataat ttgtgatttt tccggaatca ttcaacagaa aagatgaaga aaagcacaca
     5161 gcatcagtta taacttattt ttcacaattt acagaagaaa aatttttaaa cgatgaacca
     5221 gaaaccttcc atcccaaaag gaacacgcga tttctcgccc gaagaaatga caaaacgcaa
     5281 ttacattttt gataccatca aaagtgtttt tcgattgtat ggatttcagc aaatagaaac
     5341 tcctgctatg gaaaacctat ccacattact gggaaaatat ggagaagaag gcgataaatt
     5401 gttgttcaaa gttctcaatt ctggagattt tctttcggga atttccgatg cagaactgtt
     5461 ggaaagaaat gctgcccgtc tgtcgggtaa aatttgcgaa aaagggcttc gctatgattt
     5521 gactgttcct tttgcccgtt ttgtggtcca aaatcgggat aaaatttcgt tcccgttcaa
     5581 aaggtaccaa atccaacccg tttggcgtgc cgatcgtcca caaaaagggc gttatcgtga
     5641 attttatcaa tgcgatgcag atattatcgg tagcaattct ctatttaatg aattggaact
     5701 gatacaaatc atagaagaag ttttctgccg gttgaaaatc aatgtctgca ttaaaatcaa
     5761 caacagaaaa attctttcgg gtattgccga atatattgga gaacctgaaa aactgacgga
     5821 tataactacg gctatcgaca aaatagacaa gattggtatc gagaatgtga atgaagaatt
     5881 gctttcaaaa ggaatcaatc gcgaagctct cggcaaattg cagcctttgt tgcaattgaa
     5941 aggctctaat caggaaaaac ttgctcagct gaaagcttta ctttttgaat cggctgcagg
     6001 aatgaaagga attaccgagt tggaaacaat tttcgatctt tgtgaaaaag caaatgtttg
     6061 tgtcccgata gagctcgatt tgacgttggc tcgtggttta aattattata caggagctat
     6121 tttggaagtg aaggctctcg atgtggaaat tggaagcatt tcgggtggag gtcgttacga
     6181 taatttgaca ggtgttttcg gctgggaagg agtttcgggt gtaggagttt cctttggtgc
     6241 cgacaggatt tatgatgtac tttaccagtt acagcttttc cctgaaattt tggccgaaca
     6301 aaccaaaatt ctgtttgtgg tttttggaga aaaggaattg aagttcagct tgcaatgggc
     6361 acaggcatta cgcaaggaaa atatcaatac cgaaatttat gccgaaccct ccaaactga
//
LOCUS       DAOTAX010000090         6154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_10767_length_6154_cov_31.062141, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000090 DAOTAX010000000
VERSION     DAOTAX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10767_length_6154_cov_31.062141"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1397)
                     /gene="ffh"
                     /locus_tag="PLM04_07845"
     CDS             complement(78..1397)
                     /gene="ffh"
                     /locus_tag="PLM04_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HON03021.1"
                     /translation="MFESLSERLERSFKILKGEGRITEINVAETLKDVRKALLDADVN
                     YKTAKTFTDTVKEKALGQNVLTSIKPSQLMIKIVHDELAQLMGGSSVDINLKGSPAVI
                     LMAGLQGSGKTTFSAKLANLLKTKRGKNPLLVAGDVYRPAAIEQLKVLGEQLKIPVYS
                     EENNKNPVSIAEAAIKYAKLHGYDVVIVDTAGRLAVDETMMNEIAAIKKAIQPQEILF
                     VVDAMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVSLVERAQEQYNEEEARRLQKKIAKNQFDFNDFLS
                     QISQIKKMGNIKDLASMIPGMGKALKDVDIDDSAFTGIEAIIFSMTPEERENPKLLNG
                     SRKLRIAKGSGRSIVEVNRLLKQFDQVGKMMHMATVGQGKLKMKKRK"
     gene            complement(1442..3436)
                     /locus_tag="PLM04_07850"
     CDS             complement(1442..3436)
                     /locus_tag="PLM04_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HON03022.1"
                     /translation="MEEDKITGLPENAHRELKEGEEYKPILPAEKVFPEVNFRSVSTG
                     LLMAVLFSAAAAYLGLKVGQVFEAAIPIAIIAVGLSGAAKRKNALGENVIIQSIGASS
                     GVIVAGAIFTLPALYILQAKYPDISVNFMQVFLSSLLGGVLGILFLIPFRKYFVSDMH
                     GKYPFPEATATTQVLVSGEKGGDQAKPLIVAGIIGGLYDFIVATFGAWAEVFTSRVLP
                     FGAQIAEKAKLVFKVNVGAAVLGLGYIVGLKYAAIICAGSALIWFVVVPVLPLLNDQV
                     IHLLNPAFQGTLAVMSPEIIFRDFARHIGIGGIAMAGIIGVAKSWKIIMDAVGLAAKE
                     LKGGAKSLEQQKIKRTQRDISMKIIIFGIIGALLATFLFFYFGVVGNLTHAVVGILVI
                     GIIAFLFTTVAANAIAIVGTNPVSGMTLMTLILSSVIMVAVGLKGTAGMTASLIMGGV
                     VCTALSMAGGFITDLKIGYWIGTTPAKQESWKFLGTLVSAATVGGVIMILNKTYGFTG
                     ENALVAPQANAMAAVIEPLMSGVGAPWLLYGMGALIALVLNFFNVPALAFALGMFIPL
                     ELNVPLLVGGLVSWYVSGRSSNKELNRLRQEKGTLLASGFIAGGALMGVVSAALRFGG
                     IQLTNESWLQQPGSEWLALFMYVLLILYFVWDSMRIKDNK"
     gene            complement(3495..5492)
                     /gene="pulA"
                     /locus_tag="PLM04_07855"
     CDS             complement(3495..5492)
                     /gene="pulA"
                     /locus_tag="PLM04_07855"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HON03023.1"
                     /translation="MKKHLIFMMLVTGWFCSCQKTPKYASYDDYPVYDGSDLELTYTS
                     EASTFRVWAPTAAEVKLLLYDDGIDDGASRVVNMKRSENGTWKLRIKEDLKGKFYTFQ
                     VKVNDRWLDETPGMWVKATGVNGKRAAIIDMIETNPAGWEMDVRPPLKDEADIILYEV
                     HVRDFSMSPNSGMKNKGKFLAFTEHGTKNTMGQATGIDHLKELGVTHVHLLPSFDFAS
                     IDETKLNENHYNWGYDPLNYNVPEGSYSTNPYDPAVRIKEFKQMVQAFHQSGIRVVMD
                     VVYNHTFTGENSHLNLLVPGYFYRMNPDGTWSNASGCGNETASERAMMRKFIVESVVY
                     WATEYHIDGFRFDLMGVHDIETMNAVRLALDKVDPTICIYGEGWTAGPSVYPEEKRAL
                     KKNAKQLDRIAVFSDDIRDALKGSWQHPEIPGFVSGVDSLEESVKFGVVGSIQHPQID
                     YSRLIYSKEPYVNDPTQVISYVSCHDDLCLADKLRSSKPLGATNDEILRFDKLAQTIV
                     FTSQGIPFIYAGEELFRTKKGVHNSYQSPDSINQINWDNKTVFQDLYQYYKGLIALRK
                     AHPAFRLGTQENVQQYLHFLDWGIPNVVAFTLTDNAKGDTWRNILVIYNGNRKSVVVE
                     IPEGEWNLVCHDGRINLTGIAQVKTTAFVVAPSSASILYQQ"
     gene            complement(5516..>6154)
                     /locus_tag="PLM04_07860"
     CDS             complement(5516..>6154)
                     /locus_tag="PLM04_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HON03024.1"
                     /translation="EFGHSLHGMLSDCTYPSLSGTNVYRDFVELPSQIMENWASQKEF
                     LDQFAFHYQTGEKIPVELIQKIKDAENFNVGYSCLRQLGFGYLDMAWHTLSSPFEGDV
                     VDFEKQAVAKTQILPYVENTAISPTFNHIFSGGYAAGYYSYKWAEVLDADAFSVFEKN
                     GIFDQATAQSFMENILKKGGTEHPMILYKRFRGHEPSIDALLRRNGIISTNN"
BASE COUNT         1816 a         1442 c         1118 g         1778 t
ORIGIN      
        1 aaattaaaac agacaacacg gtttaggtgc aagtttgagt ttaactgata aaatcaacaa
       61 aatgtaaact ataatcgtta ttttcttttc ttcattttca attttccctg ccctactgtt
      121 gccatgtgca tcattttacc gacttggtcg aattgtttca acaatcggtt cacttccaca
      181 atacttcttc cactaccttt ggcaatgcgc aattttctgc ttccgttcag caatttcggg
      241 ttttcgcgtt cttcaggtgt catggagaaa attatggctt caataccggt aaatgcgcta
      301 tcgtcaatgt ccacatcttt caaggctttt cccatgccgg gaatcatgga agccaaatcc
      361 tttatgtttc ccatcttttt gatttgggaa atttgggaca gaaaatcgtt aaaatcaaat
      421 tgatttttgg caattttttt ctgtaatctt cgggcttctt cttcattata ctgttcctga
      481 gctctttcta ccaacgatac aatatctccc atgcccaaaa tacggtcagc cattcgttcg
      541 ggatgaaaca catccaaagc atccatcttt tcacctgttc caacaaattt aatgggcttg
      601 tcgacaaccg agcgaatgga aagtgcagct ccgccacgtg tatcgccatc caatttggtc
      661 agcaccacac catcaaaatt aagtcgatcg ttgaattcct ttgccgtatt cacagcatcc
      721 tgacccgtca tggcatccac aacaaataaa atttcctgag gctgaatggc tttttttatg
      781 gctgctattt cgttcatcat cgtctcatca actgccaaac gcccagcagt atccacaatg
      841 accacatcat aaccgtgaag tttggcatat tttatggctg cttcggcaat tgaaacagga
      901 tttttattgt tttcttcaga atacacagga attttaagct gctcacccaa aactttcaat
      961 tgctcgatag ctgcaggtcg atatacgtcg ccggcaacca acaagggatt ttttcctcgt
     1021 tttgttttca acagattggc aagtttggcc gaaaaagtag ttttacctga accttgcaaa
     1081 ccggccatca ggattacagc cggcgaacct ttcagattaa tgtccacact tgagccgccc
     1141 ataagttgag ccagttcatc gtgaacaatt tttatcatca actgactggg cttaatcgaa
     1201 gtcaatacat tttgacccaa tgctttttcc tttaccgtat cggtaaaggt tttggctgtc
     1261 ttataattga catcggcatc aagcaaagct tttctaacgt ctttcaatgt ttcggccaca
     1321 ttgatttcgg ttatccgtcc ttcacccttt aagattttaa aagatctctc aagtctttca
     1381 gaaagtgatt caaacatttt taattatcct cgtttatgaa ttaattttat tccttaaatt
     1441 tttatttatt gtcctttatt cgcatgctgt cccaaacaaa atacaaaatg agcaatacat
     1501 acataaagag cgccaaccat tccgaaccgg gctgttgcag ccaactttca ttggtgagtt
     1561 gaataccgcc aaaacgcaag gcagcgctga ctacgcccat taatgcacct ccggctataa
     1621 atcccgaagc aagcaatgtt cctttttcct gacgcaaacg gtttaattcc ttattgctgc
     1681 ttcggcccga aacataccag ctaacaagtc caccaaccag caagggaaca tttaattcca
     1741 gcggaataaa cattccgagt gcaaaagcca gcgcaggaac attgaaaaaa ttcagtacca
     1801 aagcaatcag ggctcccatg ccatacaaga gccacggcgc accaactccc gacatcaggg
     1861 gctcaattac cgctgccatc gcattggctt gaggtgcaac caaagcattt tcacctgtaa
     1921 atccgtaagt tttgttcaga atcataataa caccacccac tgtagcagcc gaaacaagcg
     1981 ttcccaaaaa tttccagctt tcctgtttgg ccggcgttgt tccaatccaa taaccaattt
     2041 tcaaatcggt gataaagcct cctgccatag acagggctgt gcaaaccaca cctcccataa
     2101 tcaatgatgc cgtcatacct gccgttcctt ttaagcccac tgctaccata atcacggaag
     2161 aaagaatcag cgtcatcaac gtcatgcccg aaacgggatt ggtacccaca atagcaatgg
     2221 cattggcagc taccgtagta aacagaaagg caataatacc tatcaccagt atcccaacca
     2281 cagcatgtgt caaattgcca actacaccaa aataaaaaaa cagaaaagta gccaacaatg
     2341 ctccgattat tccaaagata ataattttca ttgaaatatc gcgctgggta cgtttgattt
     2401 tttgctgctc gagtgatttt gctccgcctt tcagttcttt tgcggccaaa ccaaccgcat
     2461 ccataataat tttccatgat tttgccacgc cgataatacc tgccatcgca attcctccaa
     2521 tgccgatatg tcgagcaaaa tctctaaaaa taatttcagg actcatgaca gctaaagttc
     2581 cctgaaacgc cggattcagc aggtgaatta cctgatcgtt caaaagaggc aatacaggaa
     2641 caactacaaa ccaaatcaga gctgaacccg cacaaataat ggcagcatat tttaatccga
     2701 cgatatatcc caatcccaat acggcagctc cgacattaac tttgaaaacg agtttggctt
     2761 tttcggcaat ttgcgcgcca aaaggcaaaa cccgcgaagt aaaaacttca gcccaagcgc
     2821 caaaagttgc gacaataaaa tcatacaagc caccaattat gccggctaca atcaaaggtt
     2881 tggcctgatc gccacccttt tcgcccgaaa caagtacctg agtagtagct gttgcttccg
     2941 gaaaaggata cttgccgtgc atatccgaaa caaagtattt acgaaatggt atcagaaaga
     3001 ggatacccaa tacgccaccc aatagcgaac tgagaaaaac ctgcataaaa ttcaccgaaa
     3061 tatcgggata tttggcttgc agaatatata aagcgggtaa agtaaaaata gcgcctgcca
     3121 caataacgcc cgaacttgcc ccaatggact ggataatgac gttttctccc aaagcatttt
     3181 ttcgctttgc agctcccgaa agtccaactg cgataatggc tatgggaata gctgcttcaa
     3241 aaacctgtcc gacttttaaa cccagatagg ctgcagctgc cgaaaaaaga acagccatta
     3301 gtaatccggt cgaaacagag cgaaaattta cttccggaaa aaccttttcc gcagggagaa
     3361 tcggtttgta ttcttcccct tctttcaatt cccggtgtgc attttccggt aatcctgtaa
     3421 ttttgtcctc ttccatttta tgatttcgtt acgttcgttt tttcaactga aattaattct
     3481 caattctgtt atttttactg ctgatacaaa atacttgcag aagaaggagc cacaacaaaa
     3541 gcggttgttt tcacctgagc aatgccggtc aaattaatcc gtccgtcgtg gcaaaccaaa
     3601 ttccattctc cttcgggaat ttcgaccaca acagattttc gattgccatt gtaaataacc
     3661 agaatattgc gccaagtatc tcctttggca ttatcggtaa gcgtaaacgc aactacattg
     3721 ggaattcccc aatccaaaaa atgcaaatat tgttgaacat tctcttgtgt gccaagtctg
     3781 aaagcaggat gagctttgcg cagagcaatt aatcctttat agtattgata caaatcctga
     3841 aaaacggtct tgttgtccca attgatttgg ttgatggaat cgggcgactg ataagaattg
     3901 tgaacccctt tttttgttcg gaacaattct tctccggcat aaataaaagg aatgccttga
     3961 gaagtaaaaa caatggtttg agccagtttg tcgaatcgca atatttcatc attggttgct
     4021 cctaaaggtt tggaggaacg caacttgtcg gccaagcaca aatcgtcgtg gcaggaaaca
     4081 tagctgatta cctgggtagg atcatttaca tacggctctt tagaataaat cagtctggaa
     4141 taatcaattt ggggatgctg aatggaaccg actactccaa attttacgct ttcctccaaa
     4201 ctgtccactc ccgaaacaaa gccgggaatt tcgggatgct gccagctacc tttgagtgca
     4261 tcgcgtatgt cgtcgctaaa aacggcaatt ctatccaatt gcttggcatt ttttttcaga
     4321 gctctttttt cttctggata taccgatgga ccagctgtcc aaccttctcc ataaatacag
     4381 atagtaggat ctactttgtc gagtgccaac cgtacggcat tcatggtttc aatatcgtga
     4441 acgcccatta aatcgaacct gaaaccatcg atatggtatt cggtagccca ataaacgact
     4501 gattcgacga taaatttccg catcatggca cgttcggaag ccgtttcatt tccacatccg
     4561 gatgcatttg accatgttcc gtcagggttc atgcgataaa aatatcccgg aaccagcaaa
     4621 ttgagatgcg aattttctcc ggtaaaagta tggttgtaaa ccacatccat taccacgcga
     4681 attcctgatt gatgaaaagc ttgaaccatt tgcttgaatt ctttaattcg cacagccgga
     4741 tcataaggat tggtggaata acttccttcg ggtacattgt aatttaatgg atcgtatccc
     4801 caattataat gattttcatt aagttttgtc tcatcaatgg atgcaaaatc aaagctgggc
     4861 aataaatgaa catgagtaac tccaagttct tttaaatggt ctattccggt agcctgaccc
     4921 atcgtatttt ttgtcccatg ttcggtaaaa gcaagaaatt tacctttatt tttcattccc
     4981 gaattggggg acatggaaaa atcacgaaca tgtacctcat acagtatgat atcggcttcg
     5041 tctttcaagg gtggtcgtac atccatttcc cagccggcag gattagtttc tatcatatcg
     5101 ataatggcag ctcttttgcc gttaacaccg gttgctttta cccacatacc gggagtttca
     5161 tccaaccatc gatcattaac ttttacttgg aaagtgtaaa atttaccttt caaatcttct
     5221 tttatccgca atttccatgt tccattttca ctgcgcttca tgtttactac tcgcgatgct
     5281 ccgtcatcaa tgccatcatc ataaagcaaa agcttgactt ccgcagccgt aggagcccat
     5341 accctgaacg tggaagcttc ggaagtataa gtcagttcaa ggtcagaacc atcatagacc
     5401 ggataatcgt cgtaagaagc atattttgga gttttttggc aagagcaaaa ccatccggtt
     5461 accaacatca taaatatcaa atgttttttc attgttcgtt atactttatt taaaatcaat
     5521 tatttgtact aattattccg tttcttctga gtaaagcatc gatagaaggt tcatgtccgc
     5581 gaaaacgttt gtataaaatc atgggatgct cggtaccacc tttctttaag atattttcca
     5641 taaaagattg agctgtagcc tgatcgaaaa tgccattttt ttcaaaaaca gaaaaagcat
     5701 cggcatcgag tacttcagcc catttgtaac tgtaatatcc ggcggcatac ccgcccgaaa
     5761 atatgtgatt gaaagtagga ctgatagccg tattttccac gtaaggcaaa atttgggttt
     5821 tcgccacagc ctgtttttca aagtctacca cgtcgccttc aaaaggtgaa gaaagtgtat
     5881 gccaagccat atccaaatat ccaaaaccaa gttgacgcaa gcaggaatag ccgacattaa
     5941 aattttcggc atcctttatt ttttgaatca actcaaccgg aattttttca ccggtctgat
     6001 agtgaaaagc aaactgatcc agaaattcct tttgggaagc ccaattttcc atgatttgtg
     6061 agggcaattc cacaaaatcg cgataaacat ttgttcccga aagcgatggg taagtacaat
     6121 cggaaagcat tccatgtagc gaatgtccaa attc
//
LOCUS       DAOTAX010000091         6086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_10931_length_6086_cov_32.057702, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000091 DAOTAX010000000
VERSION     DAOTAX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10931_length_6086_cov_32.057702"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLM04_07865"
     CDS             complement(<1..181)
                     /locus_tag="PLM04_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03025.1"
                     /translation="MIQKFRGKYKVVKTIGCATTQHEIERLEQLVREEMNRYKIIVET
                     FIFYIFRYLITGLCPK"
     gene            312..668
                     /locus_tag="PLM04_07870"
                     /pseudo
     CDS             312..668
                     /locus_tag="PLM04_07870"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1032..3788)
                     /locus_tag="PLM04_07875"
     CDS             complement(1032..3788)
                     /locus_tag="PLM04_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HON03026.1"
                     /translation="MKKAFLFNLIVLLFFLPSKALPIKKEFLIGVSQCSDDAWRRTMN
                     EEILREASFYPNLKVKIKTAFDSNQKQIRDIESFIEDKVDILIVAPNEAIPLTPVIEN
                     AMKCSIPVILVDRKINSDNYTAFVGADNYQIGKEVGIYVANLLKRKGNIVEITGLKGS
                     TPAVERHKGFRSVIDNYPQIKIVYENDGGWLRDEAREKMKNAIRLNIPIDLVFAHNDE
                     MATGVYEAYQVYNEKEKPKIIGIDALPGTNGGIQKVLDGILDATFIYPTGGEKAVQIA
                     VRILRHEPYDKENILYTAVVDKTNARVIKLQTDQIIQHQNRIAQLNQVLNKNLSQYST
                     QRIFLVLSLTVLFVILLLLALLFKSYKHKNKVNRELEIRNTAINRQKEELAEQRDQLL
                     MLSKNLEEATQAKLVFFTNISHEFKTPLTLILGPLDRLQEKENLSEEGKQLILLMRKN
                     VHLLLKLIDQIIDFRKTETGKMQMTFTFGDLKLFISDICQSFRFLAQKKHIHFNFFSD
                     GDDFVLWFDSDKMEKICYNLISNAFKFTPENGKIEVKLSKYALNDEFFAKIIVSDSGI
                     GMPNSQLDKIFDRFFKIGNNTQNSGIGLHLTKMLVELHNGIIEVQSVEGKGSTFTVTI
                     PFKQKDIAMSEQYPDINFERNYQEKINYMDVSDEINDEYNAEKPLILLVDDNADVRNF
                     IKTLLQNEYTIIEAKDGQSGLLKAMKYVPDLIVSDVMMDKMNGFELCKAVKENISTSH
                     IPVILLTAYAMDEQRSLGFESGADAYIPKPFNENLLKIRIRKTIENRKKLKEYFQNHL
                     AFGDKKELLNEVDNNFLERFRRIIEENLMDTELNIDDIGKSMGLSRVQLYRKVKSLTN
                     YSPNEFIRIIRLKTAEQMMITGEKSISEIAYDTGFTSPSYFTKCFKEYFNENPTDFLK
                     RIKN"
     gene            complement(3864..4754)
                     /locus_tag="PLM04_07880"
     CDS             complement(3864..4754)
                     /locus_tag="PLM04_07880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HON03027.1"
                     /translation="MKDIIVGIGEILWDVLPTGKVLGGAPANFAYHISQLGFDGFAVS
                     AIGNDELGEEIIENLHRKNMNFIIEKVSFPTGTVQVTLNDAGIPCYEICENVAWDNIP
                     FTEEMKKLAQETKAVCFGSLAQRHPVSRQTIQQFLDIVPQEALKIFDVNLRQHFYSKE
                     LIDYSLKKCNILKINDEEILLLSQLFDWNEKSDIDICKRLLRDYSLDMIVLTKGTNGS
                     CVVTPHEISSKPTPIVEVADTVGAGDAFTAGFVAGILKGKSVPDAHELAVEISAFVCT
                     QQGAMPTLPQELINKISSPV"
     gene            complement(4782..>6086)
                     /locus_tag="PLM04_07885"
     CDS             complement(4782..>6086)
                     /locus_tag="PLM04_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HON03028.1"
                     /translation="VAINGGLLFGLNMAGISGAVDMIKGEFSLTDSGLGTVAGLLTLG
                     CLLGALFTGNFAEKYGRKKVMISTAIIYIVSALGCSFAESSGMLIVFRVLSGLAVGAS
                     SVVCPMYISEIAPAYNRGRLVSFNQFAITIGILLAYVFDYILAGLGENSWRYMLLIPA
                     IFGVLYLIFLLLKFPESPRWLLSKNKKEEAVKIMKAIGGDELIATELPEMEKMINADI
                     RKEKVKFIELFQGKIGKIVLIGTLLAAFQQITGINAVIMFAPDIFQAAGSAKMDSLMQ
                     SMIVGLVNFLMTVVALWLVDKSGRKSLLFWGAIGMILSLAYLTFEFSRPLQNSTGVLI
                     ALLVYISFFAASFAPVMWVVISEIYPARIKGLAMSFSTAVSWLCTFLTVYFAPVIQGK
                     LGFEYLFGIFCAFSLLAAVFVKFWIPETKGKSLEIIEKELGL"
BASE COUNT         2023 a         1190 c          936 g         1937 t
ORIGIN      
        1 gtttggggca aagccccgtt attaaatatc tgaaaatata gaatataaat gtttctacaa
       61 ttattttgta acgattcatt tcttctctga ccaattgttc caacctttca atttcgtgct
      121 gcgtggtggc acaaccaatg gttttcacta ctttatactt tcctcgaaac ttctgtatca
      181 cttgtacaga ttgacttcca gatcgatttt ttacttttcg gataaacata ataataattt
      241 tgcaacaccc aaaattaaaa tattattatt gatattcaaa tacttataag ggtgttgcaa
      301 aaaaatgtgg aaaacaggaa aaacgaagta tgatatacga gaaacgggga acaatcccca
      361 atcgtacaga tatctctcaa agaccgcctg aagttgaaca acgagaacgt tttgatgatt
      421 tggaaattga cctgattata ggcaaaaatc acaaagacac taccttaacc atcaatgaca
      481 gagctaccgg attagggaaa cttcgtaagt tagaaagcaa agatgccgaa caacttgcaa
      541 tggctaccat tgattgttta atggaatggg aaccactcct taaaacaatc actccagaca
      601 acggaaaaga gtttgccgct catcagatgg atagcccaac agttaaatat cgatttcttc
      661 tttgctaaac catatgcaag ctggcaaaaa agaggatcga atgaaaatta taatcgcctc
      721 gttaggcaat atataccgaa aaaagtcgat tttgaatgta tctctcagga ttatattatt
      781 tgtcgaaacg caaacgtaaa ttcaagtgat taatgcgact ttttaagggg aaatgaaagg
      841 aaagggaaat aatttttttg aagacaaaga aaaaaactct tcttccccct tccctgttga
      901 aataaatgct ttactgcgcc ctcttccatt ttaaaaagtt acaagatgaa acatttttat
      961 agtataacaa aagtacacaa tttgggcaat gatacaacag gtgtgcaaac aaaaattgaa
     1021 ataatttaaa atcaattttt aatacgttta aggaaatcgg taggattttc attgaaatac
     1081 tccttaaaac atttggtgaa ataggaaggt gaagtaaaac cggtatcgta agcaatttcc
     1141 gaaatacttt tttcacctgt aatcatcatt tgctcggcag tctttaacct tatgattcta
     1201 ataaattcat tgggagaata gtttgtcaat gatttaactt ttcggtaaag ctggacgcgt
     1261 gataaaccca tagatttgcc aatatcatca atatttaatt cggtatccat caaattctcc
     1321 tcaataattc ttctgaatct ctccaaaaag ttattatcaa cttcattcaa caattctttt
     1381 ttatccccaa aagccaagtg gttctgaaaa tattccttta atttttttct gttttcaatg
     1441 gtttttctaa ttctgatttt caaaagattt tcattaaaag gcttcggtat gtaagcatcg
     1501 gcgccacttt caaatcccaa agagcgttgt tcgtccatag cataagctgt caataaaata
     1561 acgggaatat ggctggtaga aatattttct tttactgcct tgcacaattc aaatccattc
     1621 attttatcca tcatcacatc gctaacaatc aaatcgggta cgtatttcat tgctttgagc
     1681 aatccgcttt gtccatcttt ggcttcaatg atggtatatt cattttgcaa caatgttttg
     1741 atgaagttac gaacgtcagc attatcatct accaataaaa tcaaaggttt ttcagcattg
     1801 tattcatcat taatttcatc agacacatcc atgtaattaa ttttctcctg ataatttctt
     1861 tcaaagttaa tatccggata ctgttcggac atagctatat ctttctgttt gaaaggaatg
     1921 gttacagtaa aagtagaacc tttaccttcg acactttgta cttctatgat tccattgtgc
     1981 aattcgacca acatttttgt caaatgcaaa ccaattccgg aattctgagt gttgttcccg
     2041 attttgaaaa atctatcaaa aattttgtcc aactgcgagt ttggcattcc tatgcccgaa
     2101 tcggacacaa ttatttttgc aaaaaattca tcgttgagag catattttga taattttact
     2161 tcaattttac cattttccgg agtaaatttg aaagcattag aaatcaagtt ataacaaatt
     2221 ttctccattt tatcgctatc gaaccacaaa acaaaatcat caccgtcaga aaagaaatta
     2281 aaatgaatgt gcttcttttg ggctaaaaaa cggaaagact gacatatatc tgaaataaaa
     2341 agttttagat cgccaaaagt aaaagtcatt tgcatttttc cggtttcggt ttttcggaaa
     2401 tcaataattt gatcaattaa tttaagaagc aagtgcacat ttttgcgcat taaaagaatc
     2461 aattgtttac cttcctcaga taaattttct ttttcctgta atcgatccaa agggccgaga
     2521 ataagcgtta aaggcgtttt gaattcgtgt gagatgttgg taaagaaaac cagttttgcc
     2581 tgtgtagcct cttccagatt tttagaaagc atcagtaatt ggtcacgttg ttcggccagc
     2641 tcctctttct gtctgtttat agcagtattt ctgatttcca attctctatt cactttattt
     2701 ttgtgcttat atgacttgaa aagtaatgcc agcaacaata aaataacaaa aagcaccgtg
     2761 agcgacagaa caagaaaaat tcgttgcgta ctgtattgtg acaaattttt atttagcacc
     2821 tgattaagtt gcgcaatacg attctgatgc tgaataattt gatctgtttg aagttttatt
     2881 actcttgcat ttgttttatc aaccacagcc gtgtagagaa tgttttcctt gtcataaggt
     2941 tcatgacgga gaattcttac agcaatctgg actgccttct cacctccggt aggataaata
     3001 aaagttgcgt ccagtatgcc atccaaaact ttctgaatac caccgttggt acccggaagt
     3061 gcatcaattc cgataatttt tggtttttct ttttcgttgt aaacctgata agcttcataa
     3121 actcctgttg ccatttcatc attgtgagca aaaaccaaat ctatgggaat gtttaaacgg
     3181 atagcatttt tcattttttc tcttgcttca tctcttaacc aacctccatc attttcatat
     3241 acaattttta tttgaggata gttatcaatc acgcttctga aacctttatg gcgctcaaca
     3301 gctggggtag agcctttcag ccctgttatt tcaacaatat tcccttttct tttcagtaaa
     3361 ttggcaacat atattccgac ttctttccca atttgataat tatcggcacc aacaaatgcg
     3421 gtataattgt cggaattgat tttccggtca accaatatta ccggaatact acatttcatg
     3481 gcattttcaa ttactggtgt taaaggaatt gcctcattgg gagcaacaat taaaatatca
     3541 actttatctt caataaaact ttcgatatcg cgtatctgtt tttgattgct gtcaaaagcc
     3601 gttttaattt tgactttcag attgggataa aaagaagctt ccctcaatat ttcttcattc
     3661 atcgttcgtc gccatgcatc atcgctgcac tgagacacac caatcaaaaa ttcttttttt
     3721 atcggtaatg cctttgatgg taaaaagaat aataaaacga ttaaattaaa aagaaaagct
     3781 tttttcatga aacagccaaa actttcatta taggtatcaa aatgatttac aacacaaaaa
     3841 tatgattttt taccaaataa aaatcaaact ggggaagaaa ttttgttaat caattcttga
     3901 ggaagagtgg gcatggcacc ttgttgggta caaacaaacg ccgatatttc cacagccaat
     3961 tcatgagcat cgggaacgct ctttcccttg agaattcctg ccacaaaacc ggcagtgaaa
     4021 gcatcaccgg cacctaccgt atcggcaacc tcaactattg gagttggttt ggatgaaatc
     4081 tcgtggggtg taacaacaca actgccattt gttccttttg tgagcacgat catatcgagt
     4141 gaataatctc tcaaaagcct tttacaaata tctatatccg acttttcgtt ccaatcgaat
     4201 agttgagaca aaagcaaaat ttcttcatca ttgattttca gtatgttgca ttttttcaat
     4261 gaatagtcta tcaactcttt agaataaaaa tgttggcgca aattaacatc aaaaattttc
     4321 aatgcctctt ggggtacgat atctaaaaac tgctgaatgg tttgtcttga aacaggatga
     4381 cgttgagcca gagaaccaaa gcatacagct tttgtttcct gtgccaattt tttcatttcc
     4441 tccgtaaaag gaatattatc ccaagccaca ttttcacaaa tttcataaca tggaatgccg
     4501 gcatcgttca gtgtaacttg cacggtaccg gttggaaaag aaactttttc gataatgaaa
     4561 ttcatatttt ttctatgaag attctcaatg atttcttcac ccaattcatc atttccaata
     4621 gcactcacgg caaagccatc gaatcccaat tgagaaatgt gataagcaaa atttgcaggc
     4681 gctcctccta aaacttttcc tgttggtaaa acgtcccaaa gtatttctcc aattccaaca
     4741 ataatatctt tcatgttttt ttgataattt ccaaattaat tttatagacc caattccttt
     4801 tcaataattt ccagtgattt tcctttggtt tccggtatcc aaaacttcac aaaaactgct
     4861 gccaataagc taaacgcaca aaatattcca aaaagatatt caaatccaag ttttccttga
     4921 ataactgggg caaaatatac ggttagaaaa gtacataacc aacttactgc agtagaaaat
     4981 gacatggcta aacctttaat tcttgccgga taaatttcag aaataacaac ccacataact
     5041 ggcgcaaaag aagcagcaaa aaaagaaata taaaccaata aagctatcaa tacacccgtg
     5101 ctattttgta aaggacgtga aaattcaaat gtcaaatatg cgagagaaag gatcataccg
     5161 attgcgcccc agaataaaag agatttccga ccacttttat ctactaacca aagagcaaca
     5221 actgtcatta aaaaattgac caaccccaca atcatcgatt gcatcaatga atccattttg
     5281 gctgaacctg ccgcctggaa aatatcaggt gcaaacataa taactgcatt tattcctgta
     5341 atttgttgaa aagcagccaa taaagttcca attaaaacaa tttttccaat ttttccctga
     5401 aacaattcaa taaacttgac tttttcttta cgaatgtcgg cattaatcat tttttccatt
     5461 tcgggaagtt cagtagcaat caattcatct cctccaattg ctttcattat tttcacagct
     5521 tcttctttct tattttttga caataaccaa cgaggacttt caggaaactt taaaagaaga
     5581 aaaattagat acaacacacc aaaaattgca ggaatcaaga gcatatagcg ccagctgttt
     5641 tcacctaaac ctgctaaaat atagtcaaat acataagcca ataagatacc tatggttata
     5701 gcaaactgat tgaaagaaac cagtcgacct ctattgtagg ccggagcaat ttccgatata
     5761 tacatgggac aaactacaga acttgcacct acagccaatc ctgacaaaac acgaaaaaca
     5821 atcaacatgc ctgaagattc ggcaaaggaa caccccaagg ctgaaacaat ataaataata
     5881 gctgtactaa tcatcacttt ttttcgacca tatttttctg cgaaatttcc ggtaaacaaa
     5941 gctcccaaca aacatccaag tgttaacaat ccggcaactg ttccaagtcc actgtcagtg
     6001 agtgaaaatt cacccttaat catatctact gctccagaaa ttccggccat atttaagccg
     6061 aataataatc ctccattgat agcaac
//
LOCUS       DAOTAX010000092         5999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_11130_length_5999_cov_29.964334, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000092 DAOTAX010000000
VERSION     DAOTAX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11130_length_5999_cov_29.964334"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..431
                     /locus_tag="PLM04_07890"
     CDS             267..431
                     /locus_tag="PLM04_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03029.1"
                     /translation="MKKLFLLCAVVFAFVACGPKENKEAAATEDSVVVVDTVVAPVDT
                     AAVDTTVAPM"
     gene            606..1457
                     /gene="hisG"
                     /locus_tag="PLM04_07895"
     CDS             606..1457
                     /gene="hisG"
                     /locus_tag="PLM04_07895"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HON03030.1"
                     /translation="MLRIAVQTKGRLFDDSMSLLNEAGIKIELGKRLLLIPAQNFPLE
                     VLFLRDDDIPESVADGVADIGIVGENEFAEKQKNVKLIRRLGFSKCRLSLAVPKEAEY
                     SDICWFEGKTIATSYPVILSNFLKEKGIRAEIHVITGSVEIAPSIGLADAIFDIVSSG
                     STLVTNHLKEVEVVMKSEALLIANPKLSEAQKKILDELLFRIEAVKMAEDKKYVLMNV
                     PTSQLEKIIEIIPGMKSPTVMPLAKEGWNSVHAVIAEKEFWDIIGKLKACGAEGILVL
                     PIEKMIL"
     gene            1472..2197
                     /locus_tag="PLM04_07900"
     CDS             1472..2197
                     /locus_tag="PLM04_07900"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HON03031.1"
                     /translation="MNLCIDQGNSSIKMGIFDQSKLVKTFVYKNFDRKSVLSMLRNYP
                     VDRCIYSSVINKNASAVAFLKKNIAQVLLLTHETPLPIENKYQTPKTLGKDRLAAVVG
                     AGFLEPNRDVLVIDAGTAITFDFMDAKRNYWGGNIDVGLELRLMALHTFAKKLPLVKP
                     VQEVPLMASNTEMALAAGALNGIIFEIDGYINALKIEYPDLSTFLTGGSLIFFDKKLK
                     NSIFVEKNLVLIGLNYILQYNVQ"
     gene            2235..3437
                     /locus_tag="PLM04_07905"
     CDS             2235..3437
                     /locus_tag="PLM04_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03032.1"
                     /translation="MQDTSSQNNTNSPYTRFGFGEINDNHNSEQRAMGGVALGVRSVS
                     SINSINPASYSVVDSMTFMFDFGVSALRSQFKDPSGKSSNFTANLDYITIQFPLSKNI
                     ALSAGIQPYSFSGYNFYTTEILTIPNNSNTPDSLNVTKSFTGSGGFSEVYMGLSIDFL
                     QHWALGVNAYYMFGTINNYRNISFSSTNYTSTTELNYIEANNFRFRYGMQYFTGFNKK
                     NDLNIGLIFEPKKNFNVKSSKINSGVLTDTTNYDTDFDLPMLLGGGIYYTYNKKLSLG
                     IDYTFQNWANTRFYGKTDSLVNRNKISIGAEYLPNSSGRKYSDHIRYRLGLNVNNPYY
                     KIDGKSLDNNFGITFGIGLPLPYSRTMLNTSFEYGKIGNYSSLREDYFKFTFNVCVNE
                     NWFFKRKL"
     gene            3468..4841
                     /locus_tag="PLM04_07910"
     CDS             3468..4841
                     /locus_tag="PLM04_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03033.1"
                     /translation="MKTKILVLLLAAIFSTNTFAQKSKNVQTPATTENSSEQVTDSMC
                     LIYISLFNEAAKNKQYADAIGPWTLAYNECPGANRVIYTRGREILQWKLSQAKDLTSY
                     KEAFDELMAMYDKRIKYFGDDSRYPTPWILGVKALDYITYVKGDDLKKPAYEWLEQSI
                     NGLKENSELEVLRQFIMLSYNLYKANPEHAEKFIADYLKVNDILETQIANPEYKSNEL
                     ANQIKQGLDVLFAQSGAADCQMLDKMYASKVTQNSTNLEFLNNVLSFYNRIGCIQSDV
                     YFSAALSAHKIQPSTESANALAEMSYKKKDYSGAIKFYEQATQLANNNKEKATYQYKI
                     AQIYYGDLKNYSRAREYALKSLEFNPNNGSAYLMIGIMYANSNIYDDPVLQKSVYWAA
                     VDKFIKAKQVDNDPKIIEEANKLIQTYINYFPSKEDIFFHPQLEEGKSFFVGGWIGES
                     TICRASN"
     gene            4930..5529
                     /gene="lptC"
                     /locus_tag="PLM04_07915"
     CDS             4930..5529
                     /gene="lptC"
                     /locus_tag="PLM04_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HON03034.1"
                     /translation="MFIVVMLFLSFACNRPTKPEIVQIESDPSLIPTLHATDITTVIS
                     DSGITRYRISAPQWDIYDKVPDPYWEFPYGIHLERFDMNLQVDANIHSKYARFFVNKK
                     LWELKNDVKATNLKGELFETEQLFWDQNSQKIYSDSLIKVTQATQIINAIGFESDQSM
                     NYYVFRNVKGIFPVDESKDTVPTSQSSNAGTPIRAMNAK"
     gene            complement(5561..>5999)
                     /locus_tag="PLM04_07920"
     CDS             complement(5561..>5999)
                     /locus_tag="PLM04_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HON03035.1"
                     /translation="VTLMKQTNAVIGGEGNGGVIYPASHYGRDALVGIALFLSYLAKS
                     KKTVSALKNEYPQYFISKNKIELTPSIDVDKILATIKNNYQLQYEVNDIDGVKIDFPT
                     GWVHLRKSNTEPIIRIYAEAKTKEQAEAFANAIMEEIRRIAAA"
BASE COUNT         2047 a         1059 c         1075 g         1818 t
ORIGIN      
        1 aaaaaatttt tttttcacac tgcaactaaa agtaattatt caaaagtcca acaaaactca
       61 ggcaaaaaca agaaactaat aattttactt tattgaatac gaagtattta attgtttttg
      121 agagggcaca aaatatttag aaaaataaaa atgaaaaaaa ttgaaaaaaa tatcgcaaat
      181 acttgcattt ataaagaaaa aaagatgtac ctttgcatcg attttgaatt gtaaacaaac
      241 atgtaaaact tcaaaaaatt aagaaaatga aaaaattatt tttattgtgt gctgttgtat
      301 ttgcattcgt tgcatgtgga ccaaaagaaa acaaagaagc tgctgctact gaagattctg
      361 tagttgttgt tgatactgtt gttgctccgg ttgatactgc agcagttgac acaactgtgg
      421 ctcctatgta attttaaaac aaccaaaatt tacagagctg aagtccgtta gtgttagcgg
      481 acttttttcg tttatacatg tagcgaatta taaaaaactc aaatcccact tttacttctt
      541 tacctgaata agaaattata tgtaaatttg taaaccgatt gtcataataa agaaaaaaat
      601 tgtttatgct aagaattgca gttcaaacca aaggaagact ttttgatgac agcatgtcgt
      661 tacttaacga agccggtatt aaaatagaat tgggaaaacg tcttttgctt attccggcac
      721 aaaattttcc tttagaagtt ttgtttttac gtgatgacga tattcccgaa tctgttgccg
      781 atggcgtagc cgatattggt attgttggag aaaacgaatt tgctgaaaag caaaaaaatg
      841 tcaaacttat tcgtcgattg ggattcagta aatgccgcct ctcgctggca gttcccaaag
      901 aagcagaata ttctgatatc tgctggtttg aaggcaaaac catagcaact tcttatcccg
      961 taattttgag taattttctg aaagaaaaag ggatacgtgc tgagattcat gttataacag
     1021 gttcagtaga aattgctccc agcataggtc ttgcagatgc cattttcgac attgtcagct
     1081 caggaagtac actggtaacc aatcatctga aagaagtaga ggttgttatg aagtcagaag
     1141 ccttgctgat tgcgaatcca aaactctctg aagcgcaaaa aaagatactg gacgagttac
     1201 ttttccgcat agaagccgta aaaatggctg aagacaaaaa atacgttttg atgaatgttc
     1261 caacctctca gctggaaaaa attatcgaaa taattcccgg tatgaaaagt cctaccgtca
     1321 tgccattggc taaagaagga tggaactctg ttcacgcagt aattgccgaa aaagaatttt
     1381 gggatattat tggaaaactg aaagcttgcg gtgctgaagg aattctggta ttacccattg
     1441 aaaaaatgat cctgtaaatt tctatcgaaa tatgaatctt tgcatcgatc agggaaactc
     1501 aagtatcaaa atgggaattt tcgaccaaag caaactggta aaaacttttg tatataaaaa
     1561 ttttgatcga aaatctgttt tatcaatgct ccgtaattat cctgtcgatc ggtgtattta
     1621 ctccagcgta atcaataaaa atgcttcagc cgttgcattt ttaaagaaaa atattgcgca
     1681 agtattactg ctgacacatg aaactccctt gcctatcgaa aacaaatacc aaacgcccaa
     1741 aactctgggg aaagaccggt tggcagctgt tgtcggtgca ggttttctcg agcccaatcg
     1801 tgatgttttg gtaatcgatg caggtactgc cataactttc gatttcatgg atgccaagag
     1861 aaactattgg ggtgggaaca ttgatgtcgg cttggaattg agattaatgg cgctgcatac
     1921 atttgcaaaa aaattgcctt tggtgaaacc ggttcaggaa gttcccttaa tggcctccaa
     1981 tactgaaatg gcattggctg caggcgctct gaacggaata atatttgaaa tcgacggtta
     2041 tattaatgct ttgaaaatcg aatatcctga cctttcaaca tttttaacag gcggaagctt
     2101 gatttttttt gataaaaaat taaaaaatag catctttgta gaaaaaaatt tggtgttaat
     2161 cggactcaat tatatacttc aatataatgt acaataaaaa gattttaatt gcatttttaa
     2221 tattgttttt atctatgcag gatacttcca gtcaaaacaa tacaaactca ccctatacgc
     2281 gttttggttt tggtgaaatc aacgacaacc acaacagcga acaaagagca atgggaggtg
     2341 tggctttggg ggttcgttct gtttcgagta tcaattctat caatcctgca tcctacagtg
     2401 tggttgacag tatgactttc atgttcgatt tcggagtttc ggcgttacgc tcgcaattta
     2461 aagatccaag tggaaaaagt tcaaacttta ctgccaatct cgattatatc accattcaat
     2521 ttcctctttc aaaaaacata gctttaagtg ccggcattca gccttattcg ttttcgggct
     2581 acaatttcta tactacagaa atacttacca taccaaataa tagtaatact ccggacagcc
     2641 ttaatgtaac gaaaagtttt accggatcag gtggattcag tgaagtttat atgggattgt
     2701 ctatcgattt tttgcaacat tgggcattgg gggtcaatgc ttattacatg tttgggacaa
     2761 ttaacaatta tagaaacata agcttttctt ccactaatta cacctctact acagaattga
     2821 attacataga agccaataat ttccgttttc gttatgggat gcagtatttt actggtttca
     2881 acaaaaaaaa cgatctaaat atcggtttaa tattcgaacc taaaaagaat tttaacgtca
     2941 agtcttccaa aattaattcg ggagtattga ccgacacaac caattatgac acagattttg
     3001 atcttcccat gctactggga gggggaattt attataccta caacaaaaag ctttcactcg
     3061 gcatcgacta tactttccaa aattgggcca atacaagatt ctacggcaaa acagacagtt
     3121 tggtcaatcg aaacaaaatt tccattggag cggaatattt gcccaactct tccgggagaa
     3181 agtacagcga ccatatacgt tatcgtttag gattaaatgt aaacaaccct tattataaaa
     3241 ttgacggaaa atcgcttgat aataattttg gtataacttt tggtattgga ttaccgttac
     3301 cctacagccg aacaatgcta aacacttcct ttgaatatgg aaaaattggt aattacagtt
     3361 ctttgaggga agactatttt aaatttacat ttaatgtttg cgtcaatgag aattggttct
     3421 ttaaaagaaa attataaaac aaacaaaatt ttaaaagtca taaaaaaatg aaaacaaaaa
     3481 tattagtgtt gttgttggct gcaatattct caaccaacac gtttgcccaa aaaagcaaaa
     3541 atgttcaaac accggctact acagaaaaca gttcagaaca ggtaaccgat tccatgtgtt
     3601 tgatttacat tagtcttttt aacgaagctg caaaaaacaa acagtatgca gatgccatag
     3661 gcccttggac tttggcttat aatgaatgcc ccggagcaaa cagggtaatt tatacacgag
     3721 gaagagaaat tttgcaatgg aaactttcac aagccaaaga cttaacttct tacaaggaag
     3781 cttttgatga attgatggcc atgtatgaca aaagaataaa gtattttggt gatgacagcc
     3841 gatatccaac tccttggata cttggggtaa aggctttgga ttatattact tacgtaaaag
     3901 gtgatgactt gaaaaaaccg gcttacgaat ggttggaaca atccatcaac ggactcaagg
     3961 aaaattctga attggaagta cttcggcaat ttataatgct ttcctataat ctttataaag
     4021 caaatcctga acatgccgaa aaatttattg ccgattattt aaaagttaac gatatacttg
     4081 aaactcaaat tgctaatcca gaatataaat ccaacgagct ggccaatcaa atcaagcagg
     4141 ggctggatgt gttgtttgca caaagcggtg cagcagattg tcaaatgctg gacaagatgt
     4201 atgcctcaaa agtaacacaa aattctacca atctagaatt tttaaataac gttttaagtt
     4261 tctacaatcg cataggatgc atacaatcag atgtttattt cagtgctgca ttgtctgctc
     4321 ataaaattca accttcaact gaatcggcaa atgcattggc agaaatgagt tataaaaaga
     4381 aagattattc cggtgccatt aaattttatg aacaagccac tcaactagca aacaacaaca
     4441 aagagaaagc tacctatcag tataaaattg ctcaaatata ctatggcgac ctcaaaaact
     4501 attctcgagc cagagaatat gcactgaaat cgttagaatt caatcccaat aacggaagtg
     4561 cctatttgat gataggcatc atgtacgcca attccaatat ctatgatgat ccagtattac
     4621 aaaaatcggt gtattgggcg gctgtcgata aattcataaa agcaaaacaa gttgataatg
     4681 accctaaaat tattgaagaa gccaataaat tgattcaaac ttacattaat tatttcccat
     4741 caaaagaaga tattttcttc catcctcaac tggaagaggg taaatctttt tttgtaggcg
     4801 ggtggattgg cgaatcaacc atttgcaggg caagcaatta aaatcattcg aataaataaa
     4861 ataaaggcat cccttaggga tcttatgaaa cagatattaa aaaattattt ttcaaaaagc
     4921 ataacaacta tgtttatagt tgttatgctt tttttatctt ttgcatgcaa tcgaccaact
     4981 aaacctgaaa ttgttcaaat tgaatctgat ccttctttga ttccgactct tcatgctacg
     5041 gatattacta ccgttatttc cgattcagga attacccgct accgtatttc tgctccgcaa
     5101 tgggatattt acgataaagt tccggatccc tattgggaat ttccttatgg cattcacttg
     5161 gaacgtttcg atatgaatct gcaagtagat gccaatattc acagcaagta tgcccgtttt
     5221 tttgttaata aaaaattgtg ggaactgaaa aatgatgtaa aagccaccaa tctgaaaggt
     5281 gaactttttg aaaccgaaca acttttttgg gatcagaaca gtcaaaaaat ttattccgac
     5341 agtttgatta aagttactca ggcaacccaa atcattaatg ccattggatt tgaatcggat
     5401 caatccatga attactatgt tttccgcaat gttaagggaa ttttccctgt ggatgaaagc
     5461 aaagatactg ttccgacttc ccagagcagt aatgccggca caccgattag agcaatgaac
     5521 gccaagtaaa aattttccaa aaatttgtta ttcggaaaat tcaagcagca gctatccgcc
     5581 gaatttcttc cataatggcg tttgcaaaag cttcagcctg ttctttggtt ttggcttcgg
     5641 cgtaaattct gataatcggc tcagtattcg atttgcgtaa atgtacccaa ccggtaggaa
     5701 aatcgatttt tacaccatca atatcattaa cttcgtactg aagttgataa ttgtttttaa
     5761 tggttgcaag aattttatcg acatcaatag acggcgtcaa ttcaatttta tttttagaaa
     5821 taaaatactg tggatactca tttttcaatg cagacacagt ttttttcgac tttgccagat
     5881 aagaaagaaa aagtgcgata cctaccaacg catcgcggcc ataatgactt gcaggataaa
     5941 taactccgcc attaccttca ccaccaatta cagcattcgt ttgtttcatt aaggtaacc
//
LOCUS       DAOTAX010000093         5957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_11233_length_5957_cov_47.518977, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000093 DAOTAX010000000
VERSION     DAOTAX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11233_length_5957_cov_47.518977"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PLM04_07925"
     CDS             complement(<1..1501)
                     /locus_tag="PLM04_07925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HON03036.1"
                     /translation="MTKKNGNGTEASLEKQSLGKLEATIWKAADKLRKNIDAAEYKHV
                     VLGLIFLKYISDAFEELYARLKAEEELGADPEDRDEYKAENVFFVPQEARWSFLLSKA
                     KQPDIGKFVDHAMDAIEKENPSLKGVLPKVYARQNLDPTGLGELIDLIGNIALGDTKA
                     RSADVLGHVFEYFLGEFALAEGKKGGQFYTPRSVVELLVEMLEPYHGRVFDPCCGSGG
                     MFVQSEKFVTEHQGRANDISIYGQESNQTTWRLCKMNLAIRGIDSSQVKWNNEGSFLN
                     DAHKDLKADYILANPPFNVSDWGGELLRNDARWQFGVPPVGNANFAWLQHFIYHLAPT
                     GQAGVVLAKGALTSKTGGEGEIRKALIENGLIDCIVNLPAKLFLNTQIPAALWFLSRN
                     RNNGKFRDRHDEILFIDARNLGHLINRRTRELSREDIQQIANTYHNWRNPDGNYEDVP
                     GFCASASIEKVKELDYVLTPGRYVGLPDNDDDFDFNERFTALKAELEEQL"
     gene            complement(1970..2707)
                     /locus_tag="PLM04_07930"
     CDS             complement(1970..2707)
                     /locus_tag="PLM04_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03037.1"
                     /translation="MTSQLNDIPTVTENKKLEELFLAIYFNDLEKVIEFKNQFPEFYT
                     KKDKFQVVENTTFDLTNLTFFNETIWFSGDWIDNIKTLVVKHRQRTMQMLDFWLKELG
                     QQEIHRKIEYHQYHEHFYCDDPNDYDEIIMEPITTYLKKGFREIDLRLYNRAQCFDFK
                     EVKKLLKLGAKSDIHFENDGDSSAYTRISQEVSYLATFNVIPEFEAFERKGYNQNFDT
                     TKMFGDLLGLAAHIEMCHLLDKYDSNQ"
     gene            complement(3078..3788)
                     /locus_tag="PLM04_07935"
     CDS             complement(3078..3788)
                     /locus_tag="PLM04_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON03038.1"
                     /translation="MKRILIILVVNLLFSHFLFAQENSRITTFFNDTLKGKWILESNC
                     LLEYQRCDSIGKGIEYIIFTSINSQDFVKVVTNGSWSFENGDSAKLYLPEKTIFGETW
                     VMDDYIFYSYNKICVINENIDKLFLSTNSFIGKAKSYIRDKSYSGIHNVINEPVTIYP
                     NPFVDNISLSYEIPNSIKEAAFKLYSVTGQLLKSIEIAERGQITKAFNLKELNSGIYI
                     GVVTFNNKAIKAIRLFKE"
     gene            complement(4168..5397)
                     /locus_tag="PLM04_07940"
     CDS             complement(4168..5397)
                     /locus_tag="PLM04_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HON03039.1"
                     /translation="MEVKIDTKKIQILEEISGTKTTYFRLLKEDEQQDLIKIYEKEGD
                     KITYFFYGGNSIKEIILIGYTKLPNILNLFGFGFEEKSMTNFFKYKFENQGYDKIQIS
                     DTLPRRKAKNTLYFKLDELEDLKSRIFQEQRACNDTKTILVKNFLIERYPNLSFDFQE
                     TNNNKELVLRNLNSKLIDKLTAEEVEKIGEFYVQAAKKYSRPDVVKRMTRGLQKNAQL
                     LTLQEIINKYEKLLKDNPKESIWQEFFDEYITLFDNRYYKKLDYKNIATGITKYPDLV
                     LVDIYGYVDFYELKKSSTPILQYDKSHKTFYWSKDFSMVIAQVADYLQKAKENSLSYS
                     KSIKEQTEDENNDGFEINIINPRAIIVAGTTKELNTKTKRNHFKNLRESLKDIEFVLY
                     DELLERLKNLLETVKLE"
BASE COUNT         1693 a         1148 c          975 g         2141 t
ORIGIN      
        1 tcaactgctc ttccagttcg gctttcaggg cggtgaaacg ttcgttgaaa tcgaagtcgt
       61 cgtcgttgtc gggtaaaccg acataccgac cgggcgtaag cacataatcg agttctttta
      121 ctttctctat ggatgcggaa gcgcaaaaac cgggaacatc ttcataattg ccatcgggat
      181 tccgccaatt gtggtatgta ttcgcaattt gctgaatatc ttcgcgagaa agttcacggg
      241 tgcggcggtt tatcaaatgc cccagattcc gggcatcgat aaacaatatc tcgtcatgac
      301 gatcgcgaaa tttgccgtta ttgcggtttc gactcaaaaa ccaaagtgca gccggaattt
      361 gtgtatttaa aaacagcttg gcaggaagat ttacaataca atcaatcaaa ccgttttcaa
      421 tcaaggcttt tcgtatttca ccctctccgc cggtttttga agtcaatgct cctttggcca
      481 ataccacccc cgcctgtccg gtgggcgcca agtggtaaat aaaatgctgc aaccaggcaa
      541 agttggcgtt gccaaccggc ggaacaccaa attgccatcg ggcgtcatta cgcagtaatt
      601 ctccgcccca atcgctcaca ttaaacggtg gattggcaag aatataatcg gctttcaggt
      661 ctttgtgggc atcgttgaga aacgaaccct cgttgttcca tttcacctgc gaactgtcaa
      721 tgccgcgaat cgccaggttc atcttgcaca accgccaggt tgtttggttg ctctcctgtc
      781 cgtaaatgga aatgtcgttt gcccttccct gatgttcggt tacaaatttt tccgattgaa
      841 caaacatacc gccggaaccg caacaggggt caaacacccg cccgtgatag ggctccaaca
      901 tttcaaccaa caattcaaca acactccgag gcgtgtagaa ttgcccgcct ttcttgcctt
      961 ccgcaagcgc aaattcaccc agaaaatatt caaacacatg tcccagtaca tcggcactgc
     1021 gggctttcgt atcgccaagg gcaatgttgc caatcaggtc gatcaattct cccaaaccgg
     1081 tgggatcaag gttttgtcgg gcatatactt tgggcaaaac accttttagt gaaggatttt
     1141 ccttttcgat ggcatccatt gcatggtcta caaactttcc aatgtcaggt tgttttgctt
     1201 tggataataa aaaactccaa cgtgcttcct gcggtacaaa gaaaacgttt tccgctttgt
     1261 attcgtctct gtcttcagga tcggctccca gctcttcttc ggctttcagt cgggcatata
     1321 attcttcaaa agcatcagag atgtatttca gaaaaatcaa tcccaacaca acatgtttat
     1381 attcggcggc gtcaatattc tttctcagtt tgtctgcagc tttccaaatt gttgcctcga
     1441 gcttgccgag cgattgtttt tctaaagatg cttctgtgcc gtttccgttt ttctttgtca
     1501 tttatatttt ttctttttta aaattcaaca agtttcaatt agcactcaaa agtaacaatt
     1561 tagtacggat ggtaaacaaa attgcagctt ttttgtaagc atttttcatg tgagtctgtt
     1621 cgtgcgggct tacaaatgta ctgcaacgtg cgagagattt ccttcttctt ttttgcagaa
     1681 ggaagaaaaa caaaataatt ttccatcaaa tttgcacagt cctaccaaaa accaaatctt
     1741 ttctttcgtt tttatttatc gctttcttat ctgaatatca tctgtctttt ttacacttgt
     1801 tgttaacggc tggcgcttgg cgcagtggcg ggttcacaga gcacaaaact gtcaagctgc
     1861 actgaatcac gcagggcgtg acacaaagct tcaagtttgc actgcctgtc ccgccttagc
     1921 gggatcaccc cgcctgaagg aaaacccatg ttacagatag ccaatttttt cattggttac
     1981 tgtcatattt gtctaacaag tgacacattt ctatgtgagc tgctaagcca agcaggtcgc
     2041 cgaacatttt tgttgtgtca aaattttgat tataaccttt tctttcaaac gcttcaaatt
     2101 caggaataac attgaaagtt gctaaatatg aaacttcttg tgaaattcgt gtatatgcac
     2161 tactgtcacc gtcattttca aagtgaatgt cagacttagc tcccagtttt aatagttttt
     2221 ttacttcctt aaagtcaaaa cattgggcac ggttgtataa tcttaaatca atttcacgaa
     2281 accccttttt caagtatgtt gttattggtt ccataattat ttcgtcatag tcattcgggt
     2341 catcacaata aaaatgctcg tgatattgat ggtattcaat ttttctatga atttcttgtt
     2401 gtccaagttc tttaagccaa aagtctaaca tttgcatagt tcgttgtctg tgctttacaa
     2461 ctaatgtctt aatgttgtca atccaatctc cgctaaacca aattgtctcg ttgaaaaatg
     2521 tcaagtttgt caagtcaaaa gttgtgtttt caacaacttg aaatttgtcc ttttttgtat
     2581 agaattcggg aaactgattt ttgaactcaa taaccttttc taagtcgttg aaataaatcg
     2641 caagaaataa ttcttccaat ttcttgtttt cggttactgt tggtatgtca ttcaactgac
     2701 ttgtcattac ttgttttacg tttttggttt aattttagtg tggtgtcgct tggttgctaa
     2761 cgaccgaggc tatgagaagt ggctacattg cgggggactt tcctgtcgta ctagcacaca
     2821 gttcgtgcgg gctgcaaaac ctcgaaaacg cagtattgta gccatttttc ataggcgtgt
     2881 gttaccaaac gtgcattggt tgtgaagaag ttgttttcat tgttttcatt ttctttgtaa
     2941 tattcaaact ttcgactacc caaattgtat atactttcaa aaaaaaaaag cacgtttggt
     3001 attcctttta attgatatgt ttccaaacgt gcaactttct tgttcgtttt gtttttggta
     3061 aatgatttgt tttaaattta ctctttaaaa agtctaatcg cttttatagc tttattattg
     3121 aatgttacta ctccaatata aatacccgaa ttcaattctt ttaaattgaa ggctttggta
     3181 atctgtcctc tttcggcaat ttcaatgctt ttcagcaatt gcccagttac agaatacaat
     3241 ttgaatgcag cttcttttat tgaattcgga atttcgtacg acaatgaaat attatcaaca
     3301 aatggattgg gataaattgt taccggctca tttattacat tgtgaatacc agaataggat
     3361 ttatcacgaa tatacgattt tgctttccct ataaaactgt tagtagacaa aaataatttg
     3421 tcaatattct cattgataac acatatttta ttataggaat aaaatatata atcgtccata
     3481 acccatgttt cgccaaaaat tgttttttca ggaagatata attttgcaga atctccattc
     3541 tcaaagctcc atgagccatt tgttacaact tttacaaaat cctgtgaatt tattgaagtg
     3601 aatattatat actcaattcc ttttccaata gaatcacatc gttgatattc taaaaggcaa
     3661 ttactttcta aaatccattt acccttcaaa gtgtcattga aaaatgttgt tattctactg
     3721 ttttcctgag caaaaagaaa atgtgaaaac aataggttaa cgactaatat aataagaatt
     3781 cttttcatgt tttttttaga tttagattat tgaccacacg ttacaatatt tacatcaagt
     3841 tgacctcctt gttgaacttc aaatccagca tttaattgta tcccttgacc tgcttcaaaa
     3901 ttaatactat tatttgtttt acagtaacca aaccagctgt ttgaatgcta ttaattgcat
     3961 ttgaaacttt aggtaattgt gttttattac tcaatgttat atctgaattt gtgcacaacg
     4021 cattttcgct aacgttttgc ggcttgacgc aggtagcgat ttagaagtac tatactgtca
     4081 acctgcactg aagctgaata gtagcacaaa ctttcactta accacgtcac cgccacttgc
     4141 ggcaaacggc tgttatgatg cgttttttta ttcaagtttc actgtttcaa gtaagttttt
     4201 aagtctttca agtaattcgt cataaagcac aaattcaatg tcctttaggc tttctcttaa
     4261 atttttaaaa tgatttctct ttgttttggt atttaattcc ttagttgttc ctgcaacaat
     4321 gattgcccgt gggttgatta tattaatttc aaaaccatca ttattttcat cttctgtttg
     4381 ttctttgata gattttgaat agctcaaact attttcttta gctttttgca gataatctgc
     4441 aacttgtgct attaccattg aaaagtcttt tgaccaataa aaagtcttat ggcttttgtc
     4501 gtattgtaga attggagtgc tacttttctt caattcataa aaatcaacat agccataaat
     4561 atcgacaagg acaaggtcag ggtatttggt tatccctgtt gctatgtttt tgtaatctaa
     4621 ttttttgtaa tatctattgt caaacaaagt aatgtattca tcaaaaaact cttgccaaat
     4681 gctttctttg ggattatctt ttaatagttt ttcatacttg ttaattattt cttgaagtgt
     4741 aagaagttga gcgttctttt gtagccccct agtcattctt ttaacaacat ccggacggct
     4801 atacttttta gctgcttgca cgtaaaattc cccaatcttt tctacttcct ccgctgttag
     4861 tttatcgatt aattttgaat ttaagtttct taaaactaac tctttattat tgtttgtctc
     4921 ctgaaagtca aaactaaggt ttggatatct ttcgattaaa aagtttttta caagtatggt
     4981 ttttgtgtca ttacaagctc tttgttcttg aaaaatcctt gattttaaat cttctaattc
     5041 gtctaattta aaatataagg tgtttttcgc ttttctacgg ggaagtgtgt ctgaaatttg
     5101 aattttatca tatccttgat tttcaaattt gtatttaaaa aaattggtca ttgatttctc
     5161 ttcgaaacca aaaccaaaca aatttaatat gttaggaagt tttgtgtagc cgattagaat
     5221 tatttctttt attgagtttc caccataaaa gaaatatgtg attttatctc cttccttttc
     5281 atatatcttt attaagtcct gttgctcgtc ttcttttaaa agtcgaaagt aagtagtttt
     5341 agtccctgaa atttcttcaa gaatttgaat ctttttggtg tctattttta cttccatttt
     5401 taaatcgtgt tgtgtcggtt tcgtctttta aaatgcacca caacgtctta tttacgcaat
     5461 aaatatacaa cattcgtaaa tacaaaattt aaaacatgca taaaccactt ttaagttttt
     5521 gcttattatt aatggtaaaa agcatgatat caataggata ggttattcaa aaaaattaca
     5581 tttacgaata ggtattgacc ttccttaatt gtgaggttgg aatgtcattt cacattcatt
     5641 ttctgaatca gctatttcag caaaatctca ttccttcttt ttctttctct accagcttac
     5701 tattttttaa ataatttcac aaatatacac aacaatttta ttcattcaaa acaaagaatc
     5761 aaaaattaat ctgtatttgc cggttcacat tttacatgta ctttccccaa aaggcaagag
     5821 taacataatt tcacgtacca gacctgcgaa ccggaacaat aagaagaaaa acagaacttc
     5881 catttttcac aaaatacttt tttacttagt acatgacaaa aaaattgtag aaacatttat
     5941 atttattatt ttcatat
//
LOCUS       DAOTAX010000094         5930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_11303_length_5930_cov_32.274894, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000094 DAOTAX010000000
VERSION     DAOTAX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11303_length_5930_cov_32.274894"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..591
                     /locus_tag="PLM04_07945"
     CDS             97..591
                     /locus_tag="PLM04_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HON03040.1"
                     /translation="MKKGYMYILECSDGSYYTGSTTNLELRLQQHQSGEGANHTKKRL
                     PVTLVYYEEYSRIDEAFYREKQVQGWSRKKKEALINGTPQLLSQLAVTYRDLKSSGGF
                     EKDFNCGFECLNHKGDTASSSASDTEVLRTSDTRAISLPGVLEGSEREALEETVSPSK
                     NEYK"
     gene            588..800
                     /locus_tag="PLM04_07950"
     CDS             588..800
                     /locus_tag="PLM04_07950"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HON03041.1"
                     /translation="MSEWKEIKLEEVLTFGNGNVKPKIEGTIPIYSVNGILGFGNEFN
                     YNNDETIIIGRVGAYCGSVYFENKPI"
     gene            811..1128
                     /locus_tag="PLM04_07955"
     CDS             811..1128
                     /locus_tag="PLM04_07955"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HON03042.1"
                     /translation="MLYLHNQNVKIMRNFSIIKYINLNQWAGGSSHPLVTQSLLNSLE
                     FSICINPQTQTAIASILSSLDDKIDLLHRQNATLEKMAETLFRQWFIERCPEPDEGEA
                     KEE"
     gene            1461..2132
                     /locus_tag="PLM04_07960"
     CDS             1461..2132
                     /locus_tag="PLM04_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HON03043.1"
                     /translation="MWLIDSSVGRKFIMSISGVFLILFLIFHSLMNIVVIISPESYNQ
                     ICAFLGANWYAVVGSLVLALGFVIHILYALYLTFLNLRARGHEKYAVKDTPKGVEWSS
                     QNMFVLGLIIFGFLVLHLYNFWFKMQFAELTGIHTGSFDPANGAAYVSALFNHWIYCV
                     LYIVWLAALWFHLTHGMWSAMQTLGLNNRKWLPRLKAISNVLSTIIVLVFISVPLYYL
                     AINIF"
     gene            2203..4173
                     /locus_tag="PLM04_07965"
     CDS             2203..4173
                     /locus_tag="PLM04_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HON03044.1"
                     /translation="MKQQEKPLEEIKIDPRIPEGPLAEKWTNYKAHQKLVNPSNKRRL
                     DIIVVGTGLAGASAAASLGELGFNVLSFCIQDSPRRAHSIAAQGGINAAKNYQNDGDS
                     VYRLFYDTIKGGDYRAREANVYRLAEVSGNIIDQCVAQGVPFAREYGGLLDNRSFGGA
                     QVSRTFYAKGQTGQQLLLGAYSAMSRQIAKGTVKLFPRREMVDVVIIEGRARGIIVRN
                     LVTGKIERYSAHAVVIASGGYGNAYFLSTNAMASNGSAAIQIYKKGAYFANPSFVQIH
                     PTCIPVHGDYQSKLTLMSESLRNDGRIWVPKRKEDAEAIRAGKLKPTDLKEEDRDYYL
                     ERRYPAFGNLVPRDVASRAAKERCDAGYGVGNTGLAVYLDFSDAISRLGINVIKERYG
                     NLFQMYEKIVDDNPYETPMMIYPAIHYTMGGIWVDYELQTSVKGLFCIGEANFSDHGA
                     NRLGASALMQGLADGYFVLPYTIQNYLSDQIQVPRFSTDLPEFEDTEKSVNDRVKRIM
                     NIKGKRSVDSIHRELGLIMWEYVGMSRNKEGLQIALDKIKEVRKEFWTNVYVPGEEYD
                     FNTELAKALRLSDFIEIGELIARDALMREESCGGHFREEYQTPDGEAKRNDEEFSFVS
                     CWKYNGEGEEPTLLKEPLIYEFVQRQQRNYKE"
     gene            4230..4985
                     /locus_tag="PLM04_07970"
     CDS             4230..4985
                     /locus_tag="PLM04_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HON03045.1"
                     /translation="MEKFINITLKIWRQAGPKAKGRFETYQLEKVSTDSSFLEMLDIL
                     NEKLISEHIDPIAFDHDCREGICGMCSLYINGHPHGPDTEITTCQLHMRKFKDGDTIV
                     VEPWRSAGFPVIKDLVVDRGAFDKIIQAGGYISVNTGGVPDGNAIPIPKEHADEAMDA
                     ATCIGCGACVAACKNGSAMLFVSAKVSHLALLPQGKVEAARRVKAMVAKMDELGFGNC
                     TNTGACEAECPKNISISNIARLNRQFLSAKFKD"
     gene            complement(5175..5330)
                     /locus_tag="PLM04_07975"
     CDS             complement(5175..5330)
                     /locus_tag="PLM04_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03046.1"
                     /translation="MARVLRVTANLKEAISKADVLTNRNLIEGSNERVTEQWIGNARN
                     STVTQEE"
     gene            complement(5505..>5930)
                     /locus_tag="PLM04_07980"
     CDS             complement(5505..>5930)
                     /locus_tag="PLM04_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON03047.1"
                     /translation="AIELTKQDGTFTTTLTNDNQKVPANTEYKYFTNEPFGQNWECNL
                     DMTPSSNRWTIAPVMNDEIQRFTTRLVTETEVPALAPRLMRTPTGIRVELNEPAAIEL
                     YNINGSLIEKTKATGTYTRDLNNGIYIIRINGKSTKFVK"
BASE COUNT         1705 a         1173 c         1343 g         1709 t
ORIGIN      
        1 caccgccctg aaagccgaac tggaagagca gctgaaagaa gaagcacgat tgaaccaaat
       61 cattctggaa aatttatcaa aaataaaact taccgaatga agaaagggta catgtacata
      121 ttggaatgca gcgacgggag ctattatacg ggaagcacga ccaatttgga attgcgattg
      181 caacaacatc aaagcggcga aggggcaaat cacacgaaga aacgactgcc tgtaacactg
      241 gtgtattacg aagaatattc gagaattgat gaagcatttt atcgcgaaaa acaggtgcag
      301 ggatggagcc gaaagaagaa agaagcattg ataaatggaa ctccccaatt gttgtcgcaa
      361 ttagccgtca cctatagaga tttgaaaagt agtggaggtt ttgagaaaga ttttaattgt
      421 ggtttcgagt gcctcaacca caaaggggat acggcgtctt cgagtgcctc agacaccgaa
      481 gtattgagaa cctcagacac cagagcaatt tcgctgcctg gggttctcga aggcagcgaa
      541 agagaggctc tcgaagaaac cgtatcccca tctaaaaatg aatacaaatg agtgagtgga
      601 aggaaataaa attggaagaa gtattaactt ttggtaatgg aaatgttaag cctaaaattg
      661 aaggtacaat tccaatttat agtgttaacg gtattttagg atttggaaac gaatttaact
      721 ataataatga tgaaactata attattggaa gggtaggcgc atattgtggt tcagtttatt
      781 ttgaaaacaa gcctatttag atttcggata atgctatatc tgcataacca aaacgtgaaa
      841 ataatgcgaa atttctctat tataaagtat atcaatttaa accaatgggc gggtggttca
      901 agtcacccgc tggttactca atctctatta aattctcttg aattttcaat ttgtattaat
      961 cctcaaaccc aaacggccat agcctccatt ctttccagcc tagatgataa aatagacctg
     1021 cttcaccgcc aaaatgccac gctcgaaaaa atggccgaaa cacttttccg ccagtggttt
     1081 attgagcgct gccctgagcc tgacgaaggg gaagcaaaag aggagtgaga aatgggaaat
     1141 tagatgatct attatcagta atagaatctg ggaaaagatc gaaaggatgt attgattcgg
     1201 aattaacatt tggaatccca agtattggag caaaaaacat taatggaatt ggtgtatacg
     1261 atttggcaaa aacgagttta tcaccgaaga atatggtaaa aagaagaaat taaagaattt
     1321 tttccgtatt tttttgctac tttgcattct ttgctttact tttgcagaag atttgaggga
     1381 catattaatt gtttattcaa gcaatttatt ttcaatgaaa taactgattt ataaccctac
     1441 taaattaaaa caaaaaaatt atgtggttaa ttgactcctc tgtcggacgg aaattcataa
     1501 tgtcgatttc gggtgtattt ctcatccttt ttttaatttt tcacagcttg atgaacattg
     1561 tggtgatcat atcacctgaa agttacaatc agatttgcgc ttttttggga gccaactggt
     1621 atgccgtggt tggttctttg gtcctggcgt tgggttttgt cattcatatt ttgtatgcgc
     1681 tttatctgac atttttaaat ttgcgggcac gcgggcatga aaaatatgcg gtaaaagata
     1741 cacccaaagg cgtagaatgg tcgtcgcaga atatgtttgt gttggggctg attatatttg
     1801 ggtttcttgt acttcatttg tacaatttct ggttcaaaat gcagtttgcc gagctgacgg
     1861 gaattcatac cggttccttt gaccctgcca atggagcagc ttatgtaagt gctcttttta
     1921 atcactggat ttactgtgtg ctctacattg tttggctcgc ggcattgtgg tttcatttga
     1981 cacacggaat gtggagcgct atgcagacgt tgggcttaaa caaccgcaaa tggttgccac
     2041 gattgaaagc catttctaat gttctctcta ccattattgt attggtattc atatcggtac
     2101 cactttatta tttggcaatc aatattttct gaaaattttt tatgatcgca agttgttgaa
     2161 aattttcatt ttcttttgta agcagttaaa attacactca atatgaaaca acaagaaaaa
     2221 ccattggaag aaattaagat agatccccga attcctgaag ggccattggc tgaaaaatgg
     2281 accaactaca aagcacatca gaaactggtc aatccttcca acaaacgtcg tctggacata
     2341 attgtggtcg gcaccgggct ggctggagct tcagcagctg cttcgctggg tgaattggga
     2401 ttcaacgtgt tgagtttctg cattcaggat tctccccgtc gtgcacactc cattgcagct
     2461 cagggcggta tcaatgcagc caaaaattat cagaatgatg gtgattctgt ttatcggctt
     2521 ttttacgata ccattaaggg aggcgattat cgtgctcgcg aagccaatgt ttaccggttg
     2581 gccgaagttt cgggaaatat catcgatcag tgtgtggctc aaggtgtgcc ttttgcacgc
     2641 gaatatggag gtttgctcga caatcgttct tttggtggcg ctcaggtttc acgtactttc
     2701 tatgccaaag gtcagacggg acagcaattg ttgctgggag cctattcggc catgagccgg
     2761 caaatcgcca aaggtacggt aaaactcttt ccacgccgcg aaatggtgga tgtagtcatt
     2821 attgagggtc gagcacgtgg aattattgta cgcaatctcg ttaccggaaa aatagaacgt
     2881 tattcggctc atgctgtagt gattgcttcc ggcggctacg gaaatgctta tttcctttcc
     2941 accaatgcta tggcttccaa cggttctgca gccattcaaa tatacaaaaa aggcgcttat
     3001 tttgccaatc cttcttttgt tcagattcat cctacctgca ttcctgttca cggcgattat
     3061 cagtcgaaac tgactcttat gtcggagtcg ctgcgtaacg atggaagaat ttgggtgccg
     3121 aaaagaaaag aagatgctga agccattcgt gccgggaaat taaaaccaac cgacttgaag
     3181 gaagaagatc gggattatta tctggaacgt cgttatccgg cattcgggaa tctggtgccg
     3241 cgcgatgtag cttcccgtgc cgcaaaagaa agatgcgatg caggttatgg tgtcggcaat
     3301 acaggtttgg ctgtttatct cgacttttcc gatgccattt cacgtttggg gatcaatgtc
     3361 atcaaggaac gatatggaaa tcttttccaa atgtacgaga aaattgtgga cgacaatccc
     3421 tacgaaactc ccatgatgat ttatccggcc attcattaca caatgggtgg aatttgggta
     3481 gattacgagc tgcaaacttc cgtaaaaggt cttttctgta ttggggaagc caacttttcc
     3541 gatcacggag ccaatcgttt gggcgcttcg gctttgatgc agggcttggc tgatggatat
     3601 ttcgttcttc cttataccat tcagaattat ctttccgatc agattcaggt tccgcgtttc
     3661 agtaccgatt tgcccgaatt tgaagatacc gaaaaatcgg ttaatgatcg tgtgaagaga
     3721 attatgaaca tcaagggaaa acgttctgta gattccattc atcgcgaatt ggggctaatc
     3781 atgtgggaat atgtcggaat gtcgcgcaat aaagaagggt tacaaattgc attggacaaa
     3841 atcaaggaag tgaggaaaga attttggacg aatgtttacg tgccgggaga ggagtacgac
     3901 ttcaataccg aattggcaaa agctttgcgt ttgtccgatt tcattgaaat aggagagtta
     3961 attgcacgcg atgcgttgat gcgtgaagaa tcgtgcggcg gacatttcag agaagaatat
     4021 cagacgcctg atggggaagc aaaacgtaac gacgaagaat tttcttttgt ttcctgttgg
     4081 aagtacaacg gtgaaggaga agagcccacg ttattgaaag aaccgctgat ttacgaattt
     4141 gttcagcgtc agcagcgcaa ttacaaagaa taagggaaca atttccccat acaacagatg
     4201 attcaacagt atattccaat ctcaacaata tggaaaaatt cataaatatc accttaaaaa
     4261 tctggcgaca agccgggcca aaagcaaagg gaagatttga aacctatcag ctcgaaaaag
     4321 tttcaaccga cagttctttt ctcgaaatgc tggatattct gaacgaaaaa ctgatcagtg
     4381 agcacattga tcccatcgct ttcgatcatg attgccgcga aggaatttgc ggcatgtgca
     4441 gcctctatat taacggacat ccacacgggc ccgatacaga aataaccact tgtcagttgc
     4501 acatgcgaaa atttaaagat ggcgatacca ttgtggtaga accctggcgt tcggccggat
     4561 tcccggtaat caaggatttg gtagtggaca gaggcgcatt cgacaagatt attcaggcgg
     4621 gaggttatat ttccgtcaat accggtggag tacccgatgg caatgctatc ccgattccca
     4681 aagaacatgc cgatgaagca atggatgcgg ctacctgcat aggctgtggt gcttgcgtgg
     4741 cagcttgcaa aaacggttcg gccatgttgt ttgtttctgc taaagttagt catttggcat
     4801 tgttgccaca gggaaaagtc gaagctgcaa gacgcgtaaa agcaatggtt gcaaaaatgg
     4861 acgaactcgg tttcggcaat tgcaccaata ccggagcttg cgaggccgaa tgcccaaaaa
     4921 atatttcaat ctccaacatt gcccgcctca accggcaatt tctttcagcc aaattcaagg
     4981 attgacattc tcgtttatta ctcgatagat ttcaattgag taggtagggg gtactcccct
     5041 accctctcac accactgtgc atgctctcgc acacggcggt ttcttttaat tgttttaacc
     5101 cctcctaatt gaatgtgaga tttgacaaac ctcctatcaa atgtgggtta aactttcgct
     5161 gcgtcttgcc tgatttactc ttcttgagtt acggttgaat ttcgggcgtt cccaatccat
     5221 tgctcggtta ccctctcatt agagccttct atcagatttc tgttcgtcaa aacgtcagct
     5281 ttgctgatgg cttctttcag atttgcagtc acccgcaaca cccttgccat tcgctaatgt
     5341 ttcctatcaa ctcggcacat acaggaactc gcatcctgaa agcttaatac catgcccgac
     5401 atactaaata accgcccctc tcggtaaaaa gaaaggcggt taaaaaatga aagtccgttt
     5461 tttctaattt ctattcctta acggactaat gaaagaacca agacttattt gacaaatttg
     5521 gttgacttgc cattgatgcg gataatgtaa ataccgttgt tcaggtcgcg ggtatacgtt
     5581 ccggtagctt tggtcttttc aatcaaactg ccattgatat tgtagagttc gattgcggcc
     5641 ggttcgttca gttctacgcg aataccggtt ggtgttctca tcaagcgtgg tgccaacgct
     5701 ggaacttccg tttccgtaac cagtctggtg gtgaatcgtt gaatttcatc attcattacc
     5761 ggagcaatgg tccagcgatt actgctaggc gtcatatcca aattgcactc ccaattttgt
     5821 ccgaacggtt cgttggtaaa atatttatat tctgtattgg cgggaacttt ttggttatca
     5881 ttggtcaagg tagtagtaaa agtaccatcc tgtttggtca attcaatagc
//
LOCUS       DAOTAX010000095         5670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_11953_length_5670_cov_31.261086, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000095 DAOTAX010000000
VERSION     DAOTAX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11953_length_5670_cov_31.261086"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..2442
                     /locus_tag="PLM04_07985"
     CDS             400..2442
                     /locus_tag="PLM04_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HON03048.1"
                     /translation="MKKSLLPAFMLAFVTCCGIFAQNSPSKFGNVGKEELEQNVCPID
                     SNAHAYFIFNCGLSRFTYPQTHVREDDAGENQGFVLETTKHFRIKILDESAFDWANIK
                     IPLYVSGKKKEKLSSFKASTFNINNGKVEETKFNKGNLIFESTSENWETAKAPLPNVK
                     KGSIIEVVYTVTSVFFYNLDKWNFQYSIPVLFSDFEVEIPEYFNFHQNIAGYYAIDRK
                     TERKNESLDLTYHWQDFNGRYSNTQRINFYVDISEYIGRNIPAFKEDSYLKTPKNYLS
                     AVEFELAYTKFPNSTLKYYTTSWENVIEQYLKYNDLENSIKVGFLDDIAKEINNKAKN
                     PYSKMELAFYNIANHYKWNEKNSIFTSKGFKDSYTNATGNCADINLCLVGLLQKLGLE
                     AYPVILSTQNNGFIKQTHPSLSDFNYLVALAKIDGNDYLMDATEPLSIINQLPVRCLN
                     DKGLIVKKGQANWVDMLKDASYKSQIILTYELDENLEAKGTYSSILNNYAALEIRKNI
                     KSYTSNDEYFKEIAKNNPGIETNEYQIEGLDSLNIPVQMKFDFSYNNAVEQAGDLIML
                     KPLLFEAMKQSPFKLSERTYPVEFNYQNKDKTTCQIKIPNGYAVESLPSSINLTLPDK
                     SAQFLFFTSIKDDYILIVSNYTRNKLLYLPEEYEALKEFFRNMVVKQNEKIVLKKL"
     gene            2514..4337
                     /locus_tag="PLM04_07990"
     CDS             2514..4337
                     /locus_tag="PLM04_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HON03049.1"
                     /translation="MANIPDSLKENADAILRYSNVSYEIKENKLIKQKSFAITLMNDN
                     AKENAYLIIPYDSYTNIKKIEGYIYNANGIKTEKLSKENIKDYAAYPSFTLFSDNRIK
                     VVGFSAKNYPVTIEYIYELESKGLLMPESWVPINDYRRSLEKAQLQIIVPDSIIQYKY
                     QNIRPTFTKETVNNNIIYSWKINGIKAKESEPFSLPFQLIMPKIFVNASKTKYFEYPV
                     EFNDWKSYGKMVFNFLTGREIVPTKSVEEINSLCKNANTELEKVKIVYAYVQNKVRYV
                     NVTKGIGGFQPLPAAEVDKYGYGDCKALSNYTRALLNAVGIKSYYAEIGNGNNKRIQF
                     PDFASVDQTNHVILFVPLKSDSLWLECTSNTIPFGYIGSGNCNRQALVVTEEGGKLID
                     TPSYNEFDNLLSISGEIFVEETGNAHLKIKKTYSNELFDEVMPFLSLSKQETEKYINK
                     SIPINGFHLTGYSFINKSSYKNIKGELAFEGDILQYAELMGKRIALKPNIISRSYFGL
                     NKKRNNNVYFSQSFTQTDTIAIHVPSGYKLEFEYPEISFSCPAGYYSTKISQKKADSF
                     LMIRKLTIKKGIYGKENYPEIYDFFKNSALADNKSCIFTKL"
     gene            4488..5516
                     /locus_tag="PLM04_07995"
     CDS             4488..5516
                     /locus_tag="PLM04_07995"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HON03050.1"
                     /translation="MISKIIAEDLINKVYSSLQDAEKIAIVVHVNPDGDAMGAALGLW
                     HYLMTIEKEPTVISPTAYPNFLSWMPGANEVLIYENDKEKADNVLASAELIFTLDFNS
                     LHRLEEMAEAVASSPAPKILVDHHLHPESFAKITISYPEISSTSEMIFRLICRMGDFA
                     NINLACAECIYTGMMTDTGSFTYNSNDPEIYIIISELIKIGIDKDEIYRKVFNTYSEN
                     RLRLMGFCLYEKMQTFPEFRTAIISLTQEELSQFNYVNGDAEGFVNIPLSVKGIDFSV
                     FLREEKNRIKISLRSQGKFPANKVAADLFGGGGHLNAAGAESYASMQETIHTLLNALP
                     DYQVFFEE"
BASE COUNT         2010 a         1040 c          950 g         1670 t
ORIGIN      
        1 caacaatgcg agaaacatgg gattgagttt tattctgtag ctgatcaagg tgcctatatc
       61 ttggatattc ccaacaaaaa aagtctttaa aacggcaatt cagccaaatt aactcaccaa
      121 cctttcaaat aatttcgccc ttatgcgtcc ccaaaaatta gaaatatctc tgaggaaaaa
      181 attttttgtt gctatttcta aaaatgtgta ggaaaattta aaattaaatt ttaactttgc
      241 tctatattaa aattttgaac ttattttaac ttttttgatg gaagagtatt catcctgtgt
      301 attccatcaa tgctcagaga aattttttct ctgggggata attattttcc aatttttcca
      361 taaaaacgac attgaattat atcaatttac aataatatca tgaaaaaaag tttattacct
      421 gccttcatgt tggcattcgt tacttgctgc ggtatatttg cacaaaattc tccttcaaaa
      481 tttggcaatg ttggcaaaga agaactggaa caaaatgttt gtcccattga ctcaaatgcc
      541 catgcgtatt ttattttcaa ttgtgggctt tctcgtttta cctatccaca gacccacgtg
      601 agagaagatg atgccggcga aaatcagggc tttgttttgg aaaccaccaa acacttcaga
      661 ataaaaattc tcgacgaatc tgccttcgac tgggcaaaca taaaaatacc tctttatgta
      721 tcaggcaaaa aaaaagaaaa actttcttct ttcaaagctt ctacttttaa tataaataac
      781 ggaaaagttg aagaaacaaa attcaacaag ggaaatctga tttttgaaag cacttcagag
      841 aattgggaaa cagccaaagc tccacttcca aacgtaaaaa aaggaagtat tattgaagtt
      901 gtttatacgg ttacttccgt atttttctat aatctcgaca aatggaattt tcagtattca
      961 attcccgtcc ttttctctga tttcgaagtt gagattccgg aatatttcaa ttttcatcaa
     1021 aatattgccg gctactatgc catcgataga aaaaccgaaa gaaaaaacga atcattagac
     1081 ttgacctatc actggcagga ttttaacgga agatacagta atacacaaag aataaatttt
     1141 tatgtggata tttccgaata tatcgggcga aacattccgg ctttcaaaga agacagctat
     1201 ctgaaaactc caaaaaacta tctttcggct gtagaatttg aactggctta cacaaaattt
     1261 cccaattcca cgcttaaata ctataccacc agttgggaaa atgtaattga acaatattta
     1321 aaatataatg acttggaaaa ttccattaaa gttggttttt tggacgatat agcaaaagaa
     1381 atcaacaata aagccaaaaa tccatattca aaaatggaac ttgcctttta taacattgcc
     1441 aatcattata aatggaacga aaaaaacagc atttttacct caaaaggctt taaggatagc
     1501 tataccaatg ctacgggaaa ttgcgccgac atcaatcttt gccttgtagg acttttgcaa
     1561 aaactcggcc ttgaggccta tccggtaata ctttccacac aaaacaacgg attcatcaaa
     1621 cagactcatc catcactttc cgattttaat tatctggtag cattggcaaa aatcgatggg
     1681 aatgactact tgatggatgc aaccgaacct ttgagcatta tcaatcaatt acctgtcaga
     1741 tgccttaacg acaaaggatt aatagtcaaa aaagggcagg caaactgggt tgatatgctt
     1801 aaagatgcaa gctacaaaag tcagattatt cttacctatg aacttgatga aaacttggaa
     1861 gcaaaaggaa catattcttc catattaaac aattatgcag cattggaaat tcgtaaaaat
     1921 attaaatcgt acacatccaa tgacgagtat tttaaagaaa tcgccaaaaa taatcccggc
     1981 atcgaaacga acgaatatca aatagaaggg ctggattcgc tgaatatacc cgttcaaatg
     2041 aaatttgatt tctcctacaa caatgcagta gaacaggccg gtgatttaat catgctaaaa
     2101 cccttgttgt ttgaagccat gaaacaatct ccgttcaaat tgtcggaaag aacctatccg
     2161 gtcgaattca attaccagaa caaagacaaa acaacctgtc aaataaaaat tccaaatgga
     2221 tatgcagtag aatccctgcc atcttctatc aaccttacct tgcctgacaa atcggcacaa
     2281 ttccttttct tcacctcgat aaaagatgac tacatactga ttgtatcgaa ttataccaga
     2341 aacaagttgc tttatttgcc ggaagaatat gaagcgttaa aagaattttt cagaaacatg
     2401 gtagtaaaac agaatgaaaa aattgtttta aagaaattgt aatcatgcga tttgtttttt
     2461 tagtcttagc ctttttttcg acattttttg tcttctctca ggaaaatctg aatgtggcaa
     2521 acatacccga ttcattgaaa gaaaatgccg atgctatcct ccgttacagt aacgtgagtt
     2581 acgaaataaa agaaaataaa ttgataaaac aaaaatcttt tgccattacc ttgatgaacg
     2641 ataatgcaaa agaaaatgct tatttgatta ttccctacga cagctataca aacattaaaa
     2701 agattgaagg ttatatttac aatgcaaatg ggataaagac cgaaaaatta tccaaagaaa
     2761 acattaaaga ttatgcggct tatccttcgt ttacactttt cagcgacaac aggataaaag
     2821 ttgtgggttt ctccgccaaa aattatccgg ttaccataga atatatttac gaattggaaa
     2881 gcaaaggcct cctcatgccc gaaagttggg tgccgataaa cgactaccgg cgatcgcttg
     2941 aaaaggcaca actgcaaatc attgttcctg atagcattat tcaatataag tatcaaaata
     3001 ttcgcccgac ctttacaaaa gaaaccgtaa ataataacat catctattct tggaaaataa
     3061 atggaataaa agcaaaggaa tcagaacctt tttctttgcc atttcagttg attatgccaa
     3121 aaatatttgt aaatgccagt aaaacaaaat attttgaata tcccgtagaa tttaacgatt
     3181 ggaaatccta tggtaaaatg gtattcaatt ttttgaccgg cagagaaata gttccaacca
     3241 aaagtgtaga agaaatcaat tctctttgta aaaacgcaaa tactgaactg gaaaaagtta
     3301 aaattgttta tgcctatgtt caaaataaag ttcgatatgt gaatgttact aagggcattg
     3361 gtggttttca gcctttaccg gctgccgaag tagacaagta tggctatgga gattgcaagg
     3421 ctctttcaaa ctataccaga gctttactga atgctgtggg gatcaagtct tactatgccg
     3481 aaatcggaaa tggaaacaat aaaagaatac agtttcccga ctttgcaagc gtagaccaaa
     3541 ccaatcatgt cattctcttt gttccattaa aatccgatag tttgtggttg gagtgtacca
     3601 gcaataccat acctttcgga tatattggct cgggaaattg caacagacaa gcattggtcg
     3661 taacagaaga aggaggaaaa ttaatcgata cgccttccta caatgaattc gacaatttgc
     3721 tttccatttc cggagaaatc tttgtagaag aaacgggaaa cgcacatttg aaaatcaaaa
     3781 aaacctattc caacgaactt ttcgacgaag taatgccatt tttaagtctg tcgaaacagg
     3841 aaacagaaaa atatatcaat aaatcgatac caataaatgg ttttcatctg acaggctaca
     3901 gctttatcaa taaaagcagc tataaaaaca taaaaggaga acttgctttt gaaggagata
     3961 tacttcaata tgcagaatta atgggtaaaa gaatagcctt gaagcccaat ataattagcc
     4021 gaagctattt tggtctgaac aaaaaaagaa acaacaatgt ttatttttcc cagtcgttta
     4081 ctcaaaccga taccatcgct attcacgttc cctcgggtta taaattggaa tttgaatatc
     4141 ccgaaatttc tttttcttgc cctgccggtt attattccac aaaaataagt caaaaaaagg
     4201 cagacagctt tctgatgata agaaaattaa caataaaaaa aggcatttat ggaaaagaaa
     4261 attatccgga aatttatgat ttctttaaaa actctgcgtt agccgacaat aaaagttgta
     4321 ttttcaccaa attgtaatcc gcgacacaat ttttttcttc agaattaaaa tatcccattc
     4381 cgagcattca tgtattttga aaaaatcgag aatgggattt ttctttggct gtgtgtaaat
     4441 tgaaaattat cacgtaaatt tgtaatttaa aatcaaaaac cgaaacaatg atttcaaaaa
     4501 ttatagctga agaccttatc aataaagttt actcgtcgct tcaggatgcg gaaaaaattg
     4561 ccatagttgt tcatgttaat cccgacggag atgcaatggg agcagcgctt ggactttggc
     4621 actatctgat gacgatagaa aaagagccaa ctgttatttc acccactgct taccccaact
     4681 tcctttcctg gatgccggga gcaaacgaag tgctgattta tgaaaacgac aaggaaaaag
     4741 ccgataacgt tcttgcttcg gcagaattaa ttttcacgct cgatttcaac agtttgcatc
     4801 gactcgaaga aatggccgaa gcagttgcaa gttcgccggc ccctaaaatt ttggttgacc
     4861 atcatctgca tcccgaatct tttgcaaaaa ttaccatttc ctatccggaa atttcttcta
     4921 ccagcgagat gatttttcgc ctgatttgcc gaatgggcga ctttgcaaat attaatcttg
     4981 cctgcgccga atgtatttat acaggcatga tgaccgatac cggaagtttt acctacaatt
     5041 ccaacgatcc ggaaatttat atcattattt ccgaactgat taagatagga atcgataaag
     5101 atgaaattta ccgaaaagtt ttcaatactt attctgaaaa ccgattgcga ttgatgggat
     5161 tttgccttta cgagaaaatg caaacttttc ctgaatttcg tacggccatc atttccctca
     5221 cgcaggaaga gttatcacaa ttcaactatg tcaacggaga tgcagaagga tttgtaaata
     5281 ttccgctttc ggttaaagga atcgattttt cagtgttttt gcgtgaagaa aaaaacagaa
     5341 ttaaaatttc gttgcgttcg caaggaaaat ttcctgccaa taaagtagct gccgaccttt
     5401 ttggcggagg cggccatctg aatgctgccg gagccgaaag ttatgcctcc atgcaagaaa
     5461 ccattcatac tttgttgaat gcattgcctg attatcaggt gttttttgaa gaataaaaaa
     5521 attattttca ctatttgaaa aacaaccata acttcttgaa gttataaatc aaaatgtttt
     5581 tcaaaaaaaa tttagtttga acaaataaaa gaccctatga aacccacatg tattgcatac
     5641 acaaacacga atgaatataa tattcagaca
//
LOCUS       DAOTAX010000096         5226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_13252_length_5226_cov_35.221814, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000096 DAOTAX010000000
VERSION     DAOTAX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_5226_cov_35.221814"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1772)
                     /locus_tag="PLM04_08000"
     CDS             complement(3..1772)
                     /locus_tag="PLM04_08000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HON03051.1"
                     /translation="MKKSNNFFKTIYQKYPYLFVLFLSLLLTIPWTMVSDFYTKGEPR
                     EAAVAQCILNTGNWILPTDYADETAYKPPLMHWMIALFSLPAGKVSETTARLPSALGL
                     IGITVSFFVFLSKRTSKKRALLATLLMLTSFEMHRSGIEARVDMLLSFFMIASLICFY
                     IWEEKGLRKFPWFISLLLAGASLIKGPVGIVLPCLVFIVYLLLLQKYSFWKIIWKNIV
                     VALPAVFILLCWYYLAYRQGGEKFLNVVYAENFGRFLGQKSETLDIAYNLGHENPFWY
                     YIPAILTGFLPWSLVAIFSLFAVSYKKLFKKLQFRKLKESFKNLKNADKLVLFSTITL
                     IVVVGFYSIPSSKRSVYIMPVYPFAAYLLVFLYEWAMKQKPQFINWTANIIFILVGFL
                     LLLMAFSRFVNLSAITSPLIKDKTLNHDIGLYSKAFQHPGVAGVLFWLFLATAFVWCL
                     KWKRTRIVQNILFTCIGLFIGLQVFLEGFAYPIYKNGYSVRPFAEKLQNKYSLKNNVF
                     VMNNLKEYPNLYALNFYLGNRFKNFEKEKPKEGYFLTGEKSLKKIRKVYGTNYQFTVL
                     ENSGNRFNDIKDVILFCRLKRIK"
     gene            complement(1863..2168)
                     /locus_tag="PLM04_08005"
     CDS             complement(1863..2168)
                     /locus_tag="PLM04_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HON03052.1"
                     /translation="MKILRRIFLNKYTLVFLIFAGYVTFFDKHNLISRWKIYQKNKEL
                     EKEIKYYDEKIKSDRTKLFELKSSNENLEKFAREHYFYKKDNEDVFIINKKDSNKNR"
     gene            2534..3406
                     /gene="fabD"
                     /locus_tag="PLM04_08010"
     CDS             2534..3406
                     /gene="fabD"
                     /locus_tag="PLM04_08010"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HON03053.1"
                     /translation="MKAFVFPGQGAQFVGMGKDLYEQSTLAKEYFEKANEILGFRITD
                     LMFEGTPEDLRQTKVTQPAVFLHSVISAFVLGEEFNPDMVAGHSLGEFSALVAARALT
                     FEDGLTLVSKRAVAMQKACELEESTMAAIIGLDDEIVEEVCDSIKDMIVVPANYNCPG
                     QIVISGTLQGVDMACEMLKERGAKRALKLSVGGAFHSPLMEPARLELSEAISQTTFSI
                     PICPVYQNVNAYPQTDPVNIKQNLIAQLTAPVRWTQTIKNMITDGADEFIELGPGDVL
                     KGLIKKINPNVIVH"
     gene            3773..4240
                     /locus_tag="PLM04_08015"
     CDS             3773..4240
                     /locus_tag="PLM04_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HON03054.1"
                     /translation="MHTWFECKIKYEKVIEEGKTLTVNETYLIDALSFTEAEKRIIQE
                     MEPYISGDFLVANIRRARINEIFAHDGGDRWYRCKVFFISLDEEKSVEKRTAVTMLVQ
                     ATSVKEAIEVLTSEMKNSLVDYELVSVSETPIMEVYPYVAEEKKVVQETSDFQ"
     gene            4269..4949
                     /locus_tag="PLM04_08020"
     CDS             4269..4949
                     /locus_tag="PLM04_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HON03055.1"
                     /translation="MSIEKNLKKIRVQIPENVQLVCVSKFQPNKAILEAYHLGERIFG
                     ESRVQELCEKQKSLPGDIQWHFIGHLQTNKVKYIVPFVDLIHAVDSLRLLEEIDRNAR
                     KIGRVVRCLLEIHIARESSKFGFLPEQLIEMLKGEGWKNLHNVQICGLMGMATYTDNQ
                     TQIRQEFRNLKLLYDQLKDSFFSNEASFRELSIGMSNDFQIAIEEGSTIVRLGTCIFG
                     ERNCTVTT"
BASE COUNT         1774 a          848 c          980 g         1624 t
ORIGIN      
        1 gtttatttta ttcgtttcaa gcggcaaaaa agaatcacat ctttaatatc attaaatctg
       61 ttccctgagt tttccaaaac agtgaattgg taatttgtac cataaacttt tcttatcttt
      121 ttcaaacttt tttctccagt cagaaaatat ccttctttcg gtttttcttt ttcaaagttt
      181 ttgaaccgat tgcccaaata aaagtttagt gcgtatagat ttggatattc cttcaaattg
      241 ttcatcacaa aaacattgtt ttttaaagaa tatttatttt gaagcttttc ggcaaaagga
      301 cggacagaat agccattttt ataaatagga taggcaaatc cttccagaaa aacttgtaag
      361 ccaataaata atcctatgca agtaaaaaga atgttctgca caattcttgt ccgtttccat
      421 ttcagacacc aaacaaaagc tgttgccaga aaaagccaga acagaacacc tgcaactccg
      481 ggatgttgaa atgcttttga atatagtccg atatcgtgat tcaaggtttt atcttttatg
      541 agtggggagg ttatggcact taaatttaca aatcgggaga aggccattaa cagtaataaa
      601 aatcccacta aaatgaaaat gatgtttgct gtccaattga taaattgagg tttttgcttc
      661 attgcccatt cgtaaagaaa aacaagcaaa taggctgcaa acggatatac aggcattatg
      721 taaacacttc gctttgaact cggtatagag taaaagccaa caaccacaat taaagttatg
      781 gtgctgaata aaaccagttt gtctgcattt ttcaaattct tgaatgattc tttcaatttt
      841 cgaaattgca attttttaaa aagctttttg tacgaaacgg caaacaaact gaaaattgcc
      901 acaagtgacc agggtaaaaa acctgtgaga attgcaggaa tgtaatacca aaaaggattt
      961 tcgtggccaa gattgtatgc aatatccaat gtttcgcttt tttgacccaa aaatcgaccg
     1021 aaattttcgg catagacaac attcagaaat ttttctcctc cttgccgata tgccaaataa
     1081 taccaacata aaaggataaa aacagccgga agagccacaa caatattttt ccagataatt
     1141 ttccagaaag aatatttctg aagcagcaaa aggtaaacaa taaaaaccaa acacggtaaa
     1201 acaatgccga ccggcccttt tatcagcgaa gctccggcca gcagcaggga aataaaccaa
     1261 ggaaattttc gcaacccttt ttcttcccaa atataaaaac aaattaatga tgcaatcata
     1321 aaaaatgaaa gtagcatgtc aactcgtgct tcaatgcccg aacgatgcat ttcaaagctt
     1381 gtaagcatta aaagtgtggc aagtaaagct ctttttttcg atgtgcgctt ggaaagaaac
     1441 acgaaaaagg aaacagtaat accaatcaat cccaatgctg aaggcaagcg ggctgtggtt
     1501 tccgatactt ttccggccgg aagtgaaaat aaggcgatca tccaatgcat caagggaggt
     1561 ttgtaggctg tttcgtctgc ataatcggtt ggtagaatcc aattgcccgt attgaggata
     1621 cattgcgcta cagcggcttc tcgtggttcg cctttggtgt aaaaatcgct caccatagtc
     1681 cacggaatag tcaacagcaa gctgagaaac aaaacaaata agtagggata tttttgatag
     1741 atggttttga aaaaattgtt cgattttttc atagatgtat gcagaaaaat tttttaaaca
     1801 tttggttttg aactgtgaaa tagtcgaaag atatttcttc aaaaagcaat tttattgttt
     1861 ttttatctgt ttttatttga atctttctta ttaataataa atacatcctc attgtctttt
     1921 ttatagaaat aatgttctct ggcaaatttt tcaagatttt cgttactgga tttgagctcg
     1981 aaaagtttgg tacgatccga ttttattttt tcatcgtaat atttaatttc tttttccagt
     2041 tctttgtttt tttggtaaat tttccagcgt gaaatcagat tgtgtttgtc gaaaaaagtt
     2101 acgtatccgg caaaaatcaa aaaaacaagg gtgtacttgt tcagaaaaat tcgacgaagt
     2161 attttcaatt tttttttgcc ggttgcagac ataaaattgt agagaaatta tggtgttgat
     2221 tgcagaaaat tatatttctt gctcttaaac ttatttagat gacaaaaata gtgaattttg
     2281 tgaatagaat ttgttaggag aaaattattt gtgtggaata aggaataatt tttttttgat
     2341 tatatttcct ttatagatca aaaaagtagc agaaatcatt cgaactgtta aatcagcata
     2401 gaagtgcgaa tttatttctt ttcggtactt ttttattaac tttgcagtcc gtttgaagaa
     2461 agttttatat ttaataagtt tattttcaat tatatcgtta tttaaaaatt taaaattatt
     2521 aaacagaaag cacatgaaag catttgtttt tccgggtcag ggggctcaat ttgtaggaat
     2581 gggcaaagat ttatacgaac agtctactct ggcgaaggaa tattttgaaa aggctaacga
     2641 aatacttgga tttcgtatta ccgatttaat gtttgaagga actccggaag acttacgaca
     2701 gactaaagtt acacaacctg ccgtttttct gcactcggta atttcggctt ttgtactggg
     2761 agaagaattt aatcccgata tggttgcagg acattcattg ggcgaatttt cagcattggt
     2821 ggcagctcgt gccctgactt tcgaagatgg attaactttg gtgtcgaaac gtgctgtggc
     2881 catgcaaaaa gcctgcgaac tcgaagaatc tacaatggcc gccattattg ggttggatga
     2941 tgaaattgtc gaagaagttt gtgattcgat taaggacatg attgtggtgc ctgccaatta
     3001 caattgcccg ggacaaatcg tcatttccgg tacattgcaa ggtgtggaca tggcttgcga
     3061 aatgcttaag gaaagaggtg cgaaacgagc actcaaactt agtgtgggtg gagcttttca
     3121 ttctccgttg atggaaccgg ccagattgga attgagcgaa gctattagtc aaactacctt
     3181 ttctattcct atttgccctg tttatcaaaa tgtgaatgct tacccacaaa ccgatcccgt
     3241 taatatcaaa caaaacttaa ttgctcaact cacagctcct gtaagatgga ctcaaaccat
     3301 caaaaacatg attactgatg gagctgatga atttattgaa ttgggacccg gagatgtctt
     3361 gaaaggattg atcaaaaaga ttaatcccaa tgttatcgtt cactaattta tagaaaagta
     3421 aattaaactt atcgaaaaag atgtatgctg taaagggttt acatcttttt ttgtaagtaa
     3481 aaatccaatt tacgtaaatc aaatgttatt taaagcaatg gcaagctgtg atagaggaaa
     3541 aaattgtttt ttgggaaaaa taaaaattaa tgaattaata aggaatgaaa cccacatgta
     3601 ttgcatacac aaacacgaat gaatataata ttcagacatg tgggttccat cagaccttta
     3661 aaaaataaat tatattctga tgaaaatcaa aatttatccc ttaaatttgt agaaactgca
     3721 acagtaagca atttaataac taaatttatt catatttaaa caattagtta agatgcacac
     3781 ttggttcgaa tgcaaaatca aatacgaaaa agtgattgaa gaaggaaaaa ccctcaccgt
     3841 gaacgaaaca tacttaatag atgctttaag ttttacggaa gctgaaaagc ggattattca
     3901 ggaaatggaa ccttatattt ctggagattt tttggtggcc aacattcgca gagcgcgcat
     3961 taacgaaatt tttgcacatg atggcggtga cagatggtac cgttgcaaag ttttctttat
     4021 ttcattggat gaagaaaaaa gtgtagaaaa gcgtacagct gttaccatgc ttgttcaggc
     4081 tacttccgta aaagaagcta tagaagttct tacttcagaa atgaaaaatt ctttggtcga
     4141 ttacgaatta gtttccgttt ccgaaacgcc cataatggaa gtttatcctt atgtggcgga
     4201 agagaaaaaa gttgttcaag aaacttcgga tttccaataa ggaaaaattt acaaaagaat
     4261 acataaacat gtccatcgaa aagaatttaa aaaaaattcg tgttcaaatt cccgaaaacg
     4321 tgcaattggt atgtgtttcc aagtttcaac ccaacaaagc catactcgaa gcgtatcact
     4381 tgggtgaaag aatttttgga gaaagcaggg tacaggaact ttgtgaaaaa caaaagtctc
     4441 ttcccggtga tattcaatgg cattttatag gacatcttca aaccaacaag gtgaagtata
     4501 ttgttccgtt tgtggatttg attcatgccg tagattcttt gagacttttg gaagaaattg
     4561 accgtaacgc ccgtaaaatt ggaagagttg ttcgttgctt gttggaaatc catattgctc
     4621 gtgaatcatc taaatttgga tttctacctg aacaacttat agaaatgttg aagggcgaag
     4681 ggtggaaaaa tttacacaat gttcaaattt gtgggttgat gggcatggca acctatacag
     4741 ataatcaaac tcaaatccga caggaattca ggaatttaaa attactatac gaccaactga
     4801 aagattcatt tttcagtaac gaagcatcct ttcgagaact ttcaataggt atgtctaatg
     4861 atttccagat tgcaatagaa gaaggcagta caatcgtcag gttgggaact tgcatttttg
     4921 gcgaaagaaa ttgtactgtt accacttaaa aaattatggc attcactccg aaaagctttt
     4981 taaaattagt ttgtttttaa acagcagtat agagtcggtc aagaaaaagc tgtgatagca
     5041 aggcagaatg aagaacttaa tccccaattt gtttgtgcgc tacatcaggc cgattgtcgc
     5101 tacaagggca aaaccgactc aacaaaaaaa ggccagaaat ggacaatcaa aatcaaaaag
     5161 gcggatattg ttatttcaat atttagaaac ccctaatggc tgatttttct gcaaaagttg
     5221 ctgttt
//
LOCUS       DAOTAX010000097         5141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_13503_length_5141_cov_32.617381, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000097 DAOTAX010000000
VERSION     DAOTAX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13503_length_5141_cov_32.617381"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..1641
                     /gene="carA"
                     /locus_tag="PLM04_08025"
                     /pseudo
     CDS             577..1641
                     /gene="carA"
                     /locus_tag="PLM04_08025"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            1684..4947
                     /gene="carB"
                     /locus_tag="PLM04_08030"
     CDS             1684..4947
                     /gene="carB"
                     /locus_tag="PLM04_08030"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HON03056.1"
                     /translation="MEKKQVAKHQYQSPKKGEIKKVLVLGSGALKIGEAGEFDYSGSQ
                     ALKALKEENIQTVLINPNIATVQTSEGIADKVYFLPVTPYFVEKVIEKERPDGIFLSF
                     GGQTALNCGVALYQQGVFEKYNVTVLGTPVQSIIDTEDRQLFNQKLSEIDVKYIKSHA
                     VTNVNDAVSAAHDLGYPVIVRAAYALGGLGSGFCNNDEELHALVEKAFSYSPQVLVEK
                     SLKGWKEVEYEVVRDRYDNCITVCNMENFDPLGIHTGESIVVAPSQTLTNSEYHKLRE
                     LSIRIIRHIGIIGECNIQYALDPFSEDYRVIEVNARLSRSSALASKATGYPLAFIATK
                     LGLGYGLPELKNSVTKTTSAFFEPALDYIVCKIPRWDLSKFNGVSHQLGSSMKSVGEV
                     MAIGRCFEEVFQKGLRMIGLGMHGFVANREYFSDSLETALSQPTDKRVFYLAQALHEG
                     YSIDKLYELTKIDRWFLFKLQNIVRLEHELQEYDSLEKLPNELLLEAKKAGFSDFQVT
                     RLITKCLPEEIEEKMKLTRVHRQRHNILPSVKQIDTLAAEYPALTNYLYLTYNGCGND
                     VQHEDDQRSVIVLGSGAYRIGSSVEFDWCGVNALTTIRKEGYRSVMINYNPETVSTDY
                     DMCDRLYFDELTFERVMDIIEMEMSKGVVVSTGGQIPNNLAMKLANAKVNILGTQAEF
                     IDMAEDRHKFSAMLDQLGIDQPRWKELTTFEEVNEFVERIGYPVLVRPSYVLSGAAMN
                     VCYDASQLEEFLKLATNVSKQHPVVISEFLERCKEIEIDAVADKGEIIVYAISEHIEF
                     AGVHSGDATTQFPPQKIYVETIRRIKNIARQIARSLNISGPFNIQFLARDNYIKVIEC
                     NLRSSRSFPFVSKVLKINFIEIATKIMLGIPVEKPEKSAFDLDYVGVKASQFSFARLQ
                     QADPILGVDMASTGEVGCLGDDSSEALLKSLLSVGITIPKKNILISSGEPKSKVDLLD
                     ACRLLYRKGYQIYATRGTHQFLMDNGVASIPVQWPDEEGEYNVKDMIAAKKFDLVINI
                     PKNVTRRELANDYTIRRSAIDFNIPLLTNARLASAFIFAFCNLTMEDVKIKSWDEYH"
     gene            5066..5141
                     /locus_tag="PLM04_08035"
     tRNA            5066..5141
                     /locus_tag="PLM04_08035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5102..5104,aa:Pro,seq:ggg)
BASE COUNT         1578 a         1036 c         1092 g         1435 t
ORIGIN      
        1 gtggaaatgg aataaatgtt gtaaaattga ccattaccat tattctctat ccacacggca
       61 gaggcataat gaaagaaatt atcctgagca cttatgttgg ataaaatgca aagaacaatg
      121 aaaagacagc caatttttcg tttcatccga aaataaagtt atgtttgttc aacaaaaaat
      181 catttagtaa taatcaaaag tattaccttc aacattttca ttgcaaaaca ctcatgagct
      241 gtcaggttaa aaacaggctt gttttgggtt ggaaagtatt tttgactcat gacactacat
      301 ttaaggtaaa acttctcaaa gtaaagccat gatttttcta cttgcaaaca aattgcaata
      361 aaaattgaaa aaaatcgatg caatcgtttg caaataaaac aaattattgt tatttaaaca
      421 ccgttcttca tccatcgagt ttcaattttt tcctctgcca atttttgctc caaccaagaa
      481 aaatcagtat ctttgcaaaa atttggatgt accatttttt caggcggatt tttttatagt
      541 tttgcctgct acttttcaaa agtttataag gaatttatgc aacaattcaa acctgtccac
      601 cttattttgg aagatggaac catcttcgag ggaaaatctt tcggttacga aaaatcggcc
      661 gcaggcgaag tagttttcaa taccgctatg gttggctatc ccgaaagttt gaccgaccca
      721 tcctacaagg gtcagttact aacgctcact tacccgctcg taggtaatta cggtgtcccg
      781 atcgacaaaa aacaaaacgg attatccacc ttttacgaat cggaaaaaat tcaggcatca
      841 gctttaatta tttccgattt ttcttttcat tacagtcatt ggaatgccgg aaaaagcctc
      901 agcgattggc tgatagaaaa tgaagttccc ggaatttttg gtgtggatac ccgcgaattg
      961 accaaactgg tacgtgaaaa aggaaccatg ttgggaaaaa tagtttttcc cggtgaagat
     1021 attgattttg tggatcccga caccgaaaat caggttgcca aagtcagctg caaagaagtg
     1081 ataacttatg gcaacgggaa gcaccgtatt ttgctggtcg attgcggagt gaaacacaac
     1141 ataattcgct gcctgctcaa acgcgacacc accgttattc gtgtgccgtg gaattatgat
     1201 tttcatcaat tggattatga tgcattgttt atttccaatg gaccggggaa tccggaattt
     1261 tgtgatgcaa cggtaaacaa tatccgaaag gccatgcaga ccgacaagcc aatattcggg
     1321 atttgcatgg gaaaccaact gctctcaatg gccggaggcg ccaaaatata caaactgaaa
     1381 tacggacaca gaagccataa ccaaccggtt caactggcag gaacacaaag agcttttatc
     1441 acctcccaga atcacggttt tgcagttgac aattcaacct taagcgacga atgggaaccg
     1501 ttgtttgtaa acatgaatga tggaacaaac gaaggaatac ggcataaaac aaagtcttgg
     1561 ttttcctgtc aatttcatcc cgaagcctct tcaggaccaa ccgatacgga atttcttttt
     1621 gatgtactca tacgtaccct gaccgaaaaa aataaatcca ttcctcaact gatagaagaa
     1681 gaattggaga aaaaacaggt tgccaagcat caataccaaa gtccaaaaaa aggcgaaata
     1741 aaaaaagtgc tggttttggg ttcgggagca ctgaaaattg gtgaagccgg cgaattcgac
     1801 tattccggtt cacaagcttt gaaagcatta aaagaggaaa acattcaaac ggtgctcatc
     1861 aatcccaaca ttgctaccgt tcaaacttca gaaggaattg ccgataaagt atatttttta
     1921 ccggttactc cctactttgt agaaaaagtt atcgaaaagg aacgtcctga tggaattttt
     1981 ctttcctttg gcggacagac ggcgctcaac tgcggcgtgg cactttatca gcagggtgtt
     2041 tttgaaaaat acaatgtaac agtgctgggt acgccggttc aatcgattat tgataccgaa
     2101 gatcgtcaac ttttcaatca aaaactttcg gaaattgatg taaaatatat caaaagccac
     2161 gccgttacca atgttaatga tgcagtatcc gcagctcacg atttgggtta tccggtaatt
     2221 gtacgggcag cttatgccct gggtggtttg ggaagcggtt tttgcaacaa cgatgaagaa
     2281 ctgcatgcat tggtagaaaa agctttttcc tattcacctc aggttttggt agaaaaatcg
     2341 ctgaaaggct ggaaagaagt cgaatacgaa gtagtaagag accgttacga caactgcatt
     2401 acggtgtgca acatggaaaa tttcgatccg ctgggcattc acaccggaga aagtattgta
     2461 gtagctcctt ctcaaacgct taccaacagc gaatatcaca aactgcgcga actttccatc
     2521 cgcatcatcc gtcacattgg tatcataggc gaatgcaaca ttcagtatgc gctggatccg
     2581 ttttcggaag attatcgagt gattgaagtc aatgcccgct taagtcggtc ttcagcgctt
     2641 gcttcgaaag ctacaggtta tccgctggca tttattgcta ctaaacttgg cttgggttac
     2701 ggacttccgg aattaaaaaa ttcggttacc aaaacaacct ccgcattttt tgagcctgca
     2761 ctcgactaca tcgtatgtaa aattcctcgt tgggatttaa gcaaatttaa cggtgtatcc
     2821 catcaactgg gcagcagtat gaaatcggtt ggtgaagtga tggccattgg tcgttgtttt
     2881 gaagaagtct ttcagaaagg attgcgcatg attggattgg gtatgcacgg ttttgttgcc
     2941 aacagagaat atttcagcga cagtcttgaa acggctctct cccagcctac cgacaaacgt
     3001 gtattttatc tggctcaagc gctacatgaa ggttattcca tcgataaact gtacgaatta
     3061 acaaaaatag atcgctggtt cctttttaag ctacaaaata tcgtccggct tgaacatgaa
     3121 cttcaggaat atgattcact cgaaaaactt ccgaatgaat tactgttgga agcaaaaaaa
     3181 gcaggctttt ccgattttca ggttacacgt ttgataacca aatgtctgcc ggaagaaatt
     3241 gaggaaaaaa tgaaactcac ccgcgttcat cgacagagac acaatatttt accatcggtg
     3301 aagcaaattg acacgctggc agccgaatat ccggctttga cgaattatct ttacctaacc
     3361 tacaacggct gcggcaacga tgttcagcac gaagacgatc aacgttcggt tattgtattg
     3421 gggtcgggag cttaccgaat cggttcttcc gtcgaattcg actggtgtgg cgtgaatgca
     3481 ctgaccacca ttcggaagga aggttatcgc tcggtaatga ttaattataa tcccgaaacg
     3541 gtttccaccg attatgacat gtgcgaccgg ctttatttcg atgaactgac ctttgaacgt
     3601 gtcatggaca ttatcgaaat ggaaatgtcg aaaggcgttg tggtgtccac aggtggacaa
     3661 attcccaata atctggccat gaagcttgcc aatgcaaaag tcaatattct tggtacgcaa
     3721 gccgaattta tcgacatggc agaagacaga cacaaatttt cagccatgct cgaccagctc
     3781 ggtatcgacc agccacgctg gaaagagttg accacttttg aggaagtaaa cgaatttgtc
     3841 gaacgaattg gttaccccgt tttggttcga ccttcgtatg tactttcggg cgctgccatg
     3901 aacgtttgtt acgatgcttc gcaattggaa gaatttttaa aactggctac caatgtttcc
     3961 aagcagcatc cggtagttat ttcggaattt ttggaacgtt gcaaggaaat agaaatagat
     4021 gctgttgccg acaaaggaga aattattgtt tatgccatat cggaacatat cgaatttgca
     4081 ggtgttcatt caggagatgc tacaacacag tttcctccgc aaaaaattta tgtggaaacc
     4141 attcgccgaa ttaaaaatat tgcccgtcag atagctcgct cactcaatat ttcgggtcca
     4201 ttcaatattc agttcctggc tcgcgacaac tatataaaag tgatagaatg caatttgcgc
     4261 tcatcgcgca gctttccgtt tgtatcgaaa gtactgaaaa tcaattttat agaaattgcc
     4321 acaaaaatca tgttgggtat tccggtagaa aaacccgaaa aatcggcttt cgatctcgat
     4381 tatgtagggg taaaggcatc gcagttctcc tttgcacgct tgcagcaagc cgacccaatt
     4441 ttgggagtcg atatggcttc taccggtgaa gttggctgtt tgggtgacga ttcgtcggaa
     4501 gctctcctaa aatccttact gtcggttgga ataaccattc cgaagaaaaa cattttgatt
     4561 tcttcaggcg aacccaagtc gaaagtagat ttgttggatg cttgtcgatt gctttacagg
     4621 aaaggatacc aaatttatgc cacacgggga actcatcagt ttttgatgga caatggcgtg
     4681 gcgagtatcc ctgttcagtg gcccgacgaa gaaggtgaat acaatgtaaa agacatgatt
     4741 gcagccaaaa aattcgattt ggttatcaat attccaaaaa atgttacgcg cagagaattg
     4801 gctaacgatt acaccattcg gcgaagtgcc attgatttta atattcctct gctcaccaat
     4861 gcacggctgg caagtgcctt tatttttgct ttctgcaacc tgacaatgga agatgttaaa
     4921 ataaaaagtt gggatgaata tcattaaaat attttcattt tcaagcaatt aaggaaatag
     4981 ggaaaagaac agttcaaaat aagcagaaaa acatttttca aatacaggga aaattgctta
     5041 attttgtatt ccgttttcaa ccattcggga tgtagctcag tccggtttag agtacgcgtc
     5101 tggggggcgt gtggtcgctg gttcgaatcc agtcatcccg a
//
LOCUS       DAOTAX010000098         4980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14053_length_4980_cov_29.308629, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000098 DAOTAX010000000
VERSION     DAOTAX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14053_length_4980_cov_29.308629"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLM04_08040"
     CDS             <1..978
                     /locus_tag="PLM04_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HON03057.1"
                     /translation="SYLSFSRAINFIRFMGSYISTFLGINRSTKLSPFFISVEPADFC
                     NLQCPECPVGVKNSLLVERGNFFDLELYRNLIEEMKGTLLHVQFYFQGEPFLNKQLTE
                     MIRYAHDRKLFTSVSTNGQFLTDETAREIVRSGLDKLIVSVDGTTQEVYEMYRKGGNL
                     QKVFDGINHVIAWKRQLKSLFPMIEMQFLVLKSNEHQMQEMKQIAKNLNVDKLSFKSA
                     QFYDFENGNSQIPANSRYVRYRLNKSGKYELKFSQPNRCSRLLGGAVINAKGEVLPCC
                     YDKQSQYSFGNIRSASFYACWHSDKANSFREQLLLDRHQFDICRNCTGR"
     gene            complement(1059..3908)
                     /locus_tag="PLM04_08045"
     CDS             complement(1059..3908)
                     /locus_tag="PLM04_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HON03058.1"
                     /translation="MNLKTILENRILILDGAMGTMIQRYRLDESAYRGERFSHWNKQL
                     KGNNDLLSLTQPDIISSIHKQYLQAGADIIETNTFNAQRISMEDYGMGEFVREMNLSA
                     ARIARQAADEFTLKNPEKPRFVAGSVGPTNKTASLPSDVNRPASRAVTFDQLVTAYKE
                     QMLALIEGGVDALLIETIFDTLNAKAAIFAAQEAMSELNKKVELMISATIADASGRTL
                     SGQTLRAFMASVAHAPLLSIGLNCSFGAKDLKPYLQEMSAHAPWFVSAYPNAGLPNQF
                     GEYDETPDTMAKQVKEYIVEGLVNIIGGCCGTTPEHIAKYRDLIANAAVRKPKEKTVN
                     LELSGLELLEIISPKIDDRQQKESIEATNSTKEKVSRNNSQEIIDISTDPAPKSLFVN
                     IGERCNVAGSRMFLRLINEKKYEEALHIARKQVEDGAQVLDINMDDAMLNAAEEMAVF
                     LNHITAEPEIARVPMMIDSSKWEVIEAGLKCIQGKSIVNSISLKEGEEDFLKKAKKVR
                     AYGAAAVVMAFDEKGQADSLERRIEICSRAYRLLTEKAGFPPQDIIFDPNVLAIGTGI
                     EEHNHYAVDFLESVRWIKQHLPYAKVSGGISNLSFSFRGNNTVREAMHSAFLYHAIKA
                     GLDMGIVNAGALPIYEEIEPELLEKVENVIFNRKATATEELIEYAEKIKNTVSEKKSD
                     KKDEWRSLPVAKRLEHALVKGIDEFLDTDLAEALTIYDTALEIIEKPLMDGMNIVGKL
                     FGEGKMFLPQVVKSARTMKKAVTILQPEIEKQKVAGTASSAGKFLIATVKGDVHDIGK
                     NIVSVVLACNNYEVIDLGVMVPSDKIIQTAINENVDLVGLSGLITPSLEEMSNVAAEM
                     EKAGLNVPLLIGGATTSKVHTAVKIAPHYHGPVVYVKDASVNTFIVSQLMNKKTHSHF
                     VSEIAAEYQEIREKQVQKTEVLSLEEARKRKPNFF"
     gene            4122..4868
                     /gene="gpmA"
                     /locus_tag="PLM04_08050"
     CDS             4122..4868
                     /gene="gpmA"
                     /locus_tag="PLM04_08050"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HON03059.1"
                     /translation="MKKLVLVRHGESVWNKENRFTGWTDVDLSEKGMQEAAKAGRLLK
                     EKGFFFDKAYTSYLKRAIKTLNIILDVMDLDWIPVEKSWRLNEKHYGMLQGLNKAETA
                     EKYGEEQVQIWRRSYDVPPAPLDMNDPRSPFLDPRYKNVDKKDLPLTEALKDTVARIL
                     PYWENNIFPSLKENNEILVVAHGNSLRGIVKVLKNISDEDIVGLNLPTGVPYVFEFDD
                     NYQVVNDYFLGDPEEIKKLMEEVANQAKKK"
BASE COUNT         1406 a          973 c         1010 g         1591 t
ORIGIN      
        1 tcttatttaa gcttttctcg tgcaatcaat ttcattcgat ttatgggttc ctatatttcg
       61 acttttttgg gtataaaccg atctactaaa ctgagccctt tttttatatc ggttgagccg
      121 gccgattttt gtaatttgca atgtcctgaa tgtcctgtgg gtgtgaaaaa ttccttattg
      181 gtggaaagag gaaatttttt tgatttggag ctttacagaa atttgataga agaaatgaaa
      241 ggaactttgc tgcatgtaca attttatttt cagggagagc cttttttgaa caaacaactc
      301 actgaaatga ttcgatatgc ccatgataga aaattattta cttccgtgtc gaccaacgga
      361 caatttttga ctgacgaaac agcacgtgaa atagtgaggt cgggattgga taaactcatc
      421 gtttcagtcg atggcaccac ccaggaagtt tatgaaatgt acagaaaagg gggaaatttg
      481 cagaaagtat tcgatggtat caatcatgtt atagcttgga aaaggcagtt gaaatcttta
      541 tttccaatga ttgaaatgca gtttttggtt ctaaagtcca acgaacatca aatgcaggaa
      601 atgaaacaaa tagcaaaaaa cttgaatgtg gacaaactta gttttaagtc cgcacagttt
      661 tacgattttg aaaatggaaa ttcgcaaatt cctgccaaca gtcgttatgt gaggtatcga
      721 ctcaacaagt caggaaaata cgaacttaaa ttttcgcagc ccaatcgatg cagtcgtttg
      781 ctgggaggag cagtaattaa tgcaaaagga gaggtgttgc cgtgttgcta tgataagcag
      841 tcgcaatact catttggaaa tatccgttct gcttcctttt atgcctgttg gcattcggat
      901 aaggcaaatt cttttcggga acagttgctc ttagaccgtc atcagtttga tatttgcaga
      961 aattgtacgg gaagataaag aattgcggct gaaaagggtt acgagttgtg agttctaagt
     1021 cgttcatgcc tgaaatacgt tgaccatttt ggttattttc agaaaaaatt tggtttacgt
     1081 tttcgagctt cttcaagtga gagcacttca gttttctgaa cttgcttttc gcgaatttcc
     1141 tgatattcgg cagctatttc ggaaacaaaa tggctatgtg tttttttgtt catcagctga
     1201 gaaacgatga aagtattgac ggatgcatct ttaacatata ccaccggtcc atgataatga
     1261 ggcgcaattt ttactgccgt atgaactttg gatgttgttg ctcccccaat aagtaaggga
     1321 acgttgagcc cggctttttc catttcggca gcaacgttgc tcatctcttc cagcgaagga
     1381 gttatcagtc cacttaatcc aaccaaatcg acattttcgt tgattgctgt ctgaataatt
     1441 ttatctgagg gtaccataac acccaagtcg ataacttcat aattgttgca agccaatacc
     1501 acagatacaa tattttttcc aatatcgtgc acatcacctt ttacggttgc aataaggaat
     1561 ttgcctgccg atgaggctgt tccggcaact ttttgttttt caatttcggg ctgaagaatg
     1621 gttacggctt ttttcatggt tcgagccgac ttcaccactt ggggcaaaaa cattttacct
     1681 tctccgaaaa gttttccaac aatgttcatc ccgtccatca agggtttttc gatgatttcc
     1741 agtgcggtat cgtaaatagt cagtgcttct gccaaatcgg tatcgagaaa ttcatcaatt
     1801 ccctttacaa gtgcatgttc aaggcgttta gcaaccggaa gcgaacgcca ttcatctttt
     1861 ttgtccgatt ttttttcgga caccgtattc tttatttttt ctgcatattc tatcagttct
     1921 tccgtagctg ttgctttccg gttgaagata acattttcca ctttttccaa aagttcaggc
     1981 tctatttctt cataaattgg cagcgctccc gcgttaacaa ttcccatatc caatccggct
     2041 tttatggcgt gataaagaaa agcggaatgc atggcctcac gaaccgtatt gtttcctctg
     2101 aaggaaaaag ataaattgct gattcccccg ctaacttttg cataaggcaa atgttgtttg
     2161 atccatcgta ccgactccag aaaatctaca gcgtaatgat tgtgttcttc tattccggta
     2221 ccaatagcca aaacattggg atcaaaaatt atatcctgtg gtggaaatcc tgctttttcg
     2281 gttagcagac gatatgctcg ggagcaaatt tcgattcttc gctccaagct gtcggcttgg
     2341 cctttttcgt cgaaagccat cacaacggca gccgcaccat aagcccgcac cttttttgct
     2401 tttttcagaa aatcttcttc tccttccttt aaactgatgg aattgacaat gctttttccc
     2461 tgaatacatt tcaaaccggc ttcaatgact tcccattttg acgaatcaat catcatggga
     2521 acacgtgcaa tttcgggctc ggcagtaatg tgattcaaaa aaacagccat ttcttctgct
     2581 gcattcagca tggcgtcatc catgttgatg tcaagtactt gcgcaccatc ttcaacttgt
     2641 tttcgggcaa tgtggagcgc ttcttcgtat tttttctcgt tgattagcct taaaaacatg
     2701 cgcgaaccgg ccacgttgca tcgttcacca atgttcacaa ataaagattt tggagccgga
     2761 tcggttgaaa tgtcgataat ttcctgacta ttatttcttg aaactttttc ttttgtcgaa
     2821 tttgtggctt caatgctctc cttttgttgc ctatcgtcaa ttttaggaga aatgatttcc
     2881 aatagttcca aaccgctcag ttccaggttt acggtttttt ctttgggttt tctgaccgct
     2941 gcattggcaa tcagatcccg atatttggca atatgttccg gagttgtacc gcagcaaccc
     3001 ccgataatgt tgaccaaacc ttcgacaata tattctttta cttgttttgc cattgtgtcg
     3061 ggcgtttcgt cgtattcacc aaactgattc gggaggccgg cattggggta tgcactgaca
     3121 aaccaaggtg catgagcgct catttcctga aggtaaggtt ttaaatcttt ggctccaaaa
     3181 gaacagttta atccgataga aagtagcgga gcatgtgcga cggaagccat aaaagcccga
     3241 agtgtttgcc cggaaagtgt acgtccgctg gcatcggcaa tggtagccga aatcattaat
     3301 tccacttttt tattcaattc cgacatagct tcttgagccg cgaaaatagc ggcttttgca
     3361 tttaaagtgt cgaaaatggt ttctatcaac agcgcatcta ctccaccttc tatcaaagcc
     3421 agcatttgtt ccttgtaagc tgttaccagt tgatcgaagg ttacggcgcg cgaagcggga
     3481 cgatttacgt cggaaggtaa ggaagccgtt ttattggttg ggcctaccga accggcaaca
     3541 aatcttggtt tttcggggtt tttgagagtg aattcatctg ctgcctgtct ggctatgcgc
     3601 gctgcactca aattcatctc tcttacaaat tctcccattc cataatcttc catcgaaatt
     3661 cgttgtgcat tgaaagtgtt ggtttcgata atatcggcac ctgcttgcaa atattgctta
     3721 tggatggaac taataatatc cggttgagtt aaagaaagaa ggtcgttgtt tcctttcaat
     3781 tgcttgttcc aatgagaaaa acgttcacct ctgtatgcgg attcgtcgag cctgtaacgc
     3841 tgaatcatgg tgcccattgc tccatccaga atcaatatgc gattttcgag aattgttttt
     3901 aaattcatag tgttgtaaaa taggaaggaa attttcaaat atatctgtcg ttttatttct
     3961 gcaaaattaa tgaatatggg tcaattctca aaaacagcaa cttatcctta taaatttcca
     4021 ttctatgatt tttattttcg cacgtttgga aatacttcga ttttcatgta attttgtcat
     4081 caataaaaaa agtgaatcac taacctctta ataagaagaa aatgaaaaaa ttagttttag
     4141 taagacatgg cgaaagcgtt tggaataagg aaaatcgctt tactggttgg acagatgttg
     4201 atttgtctga aaaaggaatg caagaagcag ccaaagcagg cagattgctg aaggaaaagg
     4261 gtttcttttt cgataaagcc tacacttcct atttgaagag agccattaaa actttgaata
     4321 tcattcttga tgtaatggat ttggattgga ttccggtaga aaaaagctgg cgtttgaacg
     4381 aaaaacatta tggaatgctt cagggtttaa acaaagcaga aacagccgaa aaatacggcg
     4441 aagaacaagt tcagatttgg agacgcagct atgatgttcc gcctgcaccg ctggacatga
     4501 atgatcctcg ttcaccgttt ttggatccac gctacaaaaa tgtggataaa aaagatttgc
     4561 ctctgacaga agctttgaaa gataccgtag ctcggattct tccttattgg gaaaacaaca
     4621 tcttcccaag tttgaaagaa aacaacgaaa ttctggttgt tgctcacggt aacagcttga
     4681 gaggtattgt aaaagttttg aaaaatattt ccgacgaaga tatcgtgggt ttaaatctcc
     4741 ccacaggtgt gccctatgtt tttgaatttg atgataatta tcaggttgta aacgattatt
     4801 ttctgggcga tccggaagaa atcaaaaaat tgatggaaga agtggccaat caggcaaaaa
     4861 agaaataaga agaattcttc tcttctaagc tataataaag cagtgcacaa ccgtactgct
     4921 tttttattat atacattaca ttaaattatt gcacaaataa attattatct ttgtctcata
//
LOCUS       DAOTAX010000099         4921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14257_length_4921_cov_29.924784, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000099 DAOTAX010000000
VERSION     DAOTAX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14257_length_4921_cov_29.924784"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1765)
                     /gene="groL"
                     /locus_tag="PLM04_08055"
     CDS             complement(137..1765)
                     /gene="groL"
                     /locus_tag="PLM04_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HON03060.1"
                     /translation="MAKEILYNHDAREQLKKGVDELANAVKVTLGPKGRNVIIEKKFG
                     APHITKDGVTVAKEIELEDPFKNLGAQLLKEVASKTSDDAGDGTTTATVLAQAIVSVG
                     LKNVTAGANPMDLKRGIDKAVSQVVKHIASQSKKVDDDYNKIEQVATVSANNDSEIGK
                     LIADAMRKVSKDGVITIEEAKGTETTIELVEGMQFDRGYISAYFVTNTEKMLVEMEKP
                     YILLYDKKISNFKELLPILEPVAQSGRSLLIIAEDVDSEALTTLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTNGVVISEEKGLQLEKATMDMLGTAEKVTVDKDNTTIVN
                     GAGSKEAIAARVSQIKSQIANTTSDYDREKLQERLAKLAGGVAVLYVGAPSEVEMKEK
                     KDRVDDALHATRAAIEEGIIPGGGVAYIRAIEALENLKGENEDEETGIEIVKRAIEEP
                     LRQIAFNAGKEGAVIVNKVKEGKGDFGYNARNEKFENFFETGVLDPAKVARIALENAA
                     SVAGMFLTTECVITEKKEENPVPQMPGGGMGDMM"
     gene            complement(1839..2111)
                     /locus_tag="PLM04_08060"
     CDS             complement(1839..2111)
                     /locus_tag="PLM04_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HON03061.1"
                     /translation="MNIKPLADRVLVKPAPAEEKTSSGIIIPDSAKEKPLKGEVVAVG
                     KGTKDEEMIVAVGDTVLYGKYAGTELEWDGEKYLIMRQSDIMGIID"
     gene            complement(2284..3192)
                     /locus_tag="PLM04_08065"
     CDS             complement(2284..3192)
                     /locus_tag="PLM04_08065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON03062.1"
                     /translation="MLSILIPVFNYSVSRLVGELHRQALQLFIDFEIIVMEDGSNEYL
                     EENRQIDKLSRCSYIVLEKNIGRSAIRNRLADMSKYGHLLFLDCDAEVCSAYFVEKYV
                     SFCREESVVVGGTAYDENFNDFAYSLRLKYGRLREARPATLRNKLELSGKSNFSAFNF
                     LIAKSIFNRIRFDETISGYGHEDTLFGHRIHELGFNVFHIDNPLIHKGLDDNPTFLFK
                     TEEGIRNLYYLYKTGRYPFLAKESSLLNSFLTLKKLSLIEFFSFLYRIFRKSIYANLC
                     SSNPSLILFDFYKLLYFCNYSQKMTR"
     gene            complement(4010..4831)
                     /locus_tag="PLM04_08070"
     CDS             complement(4010..4831)
                     /locus_tag="PLM04_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03063.1"
                     /translation="MKKHFFIVLFCWAGAFSLLTAQDTIVFKKGEIKACKITQIKAGE
                     LTCKDFSYLDGPDYVYELKMIQEIHFSNGVVQIYNLPDVSEIKEEKGSITVSRGRIRN
                     LTQDSWTPPISFKTYKEKENYYKDQLYFPKFGDPYQPWVAGIASYLIPGLGQITCGEV
                     GRGFAFMGGAAASFFVFMIGALGSSGYYNDYYYRSDADFYPFLALSGVVSLFVIDIWS
                     VVDAFHVAKINNLYQRDLHSQEQVKLQLTPYIDKNKLDFSWNHQHTMGLSLQVNF"
BASE COUNT         1567 a          985 c          787 g         1582 t
ORIGIN      
        1 ttttattacg aaatttgtat aaccaaaaag ttgcatttgt gacttgaatt cagcataaga
       61 aaaatttcag acacaaaaaa atctccgaaa actatacagt ctccggagat ttttaaccta
      121 tcgagaattt ttaaaattac atcatatcac ccattccccc acctggcatt tgaggaaccg
      181 gattctcttc tttcttttcg gtgatcacac attcggttgt caggaacata ccggccacag
      241 aggctgcatt ttccaaagca atacgggcaa ctttggcagg atcgagtact cccgtttcaa
      301 agaaattttc aaatttttcg ttgcgagcat tgtacccaaa atcgcctttg ccttctttaa
      361 ctttgtttac aattacggcg ccttcttttc cggcattgaa tgcaatctga cgaagcggtt
      421 cttcgatagc ccgtttaaca atttcgatac ctgtttcttc gtcttcgttc tcacctttca
      481 ggttttccag cgcttcgatg gcgcgaatat aagcaacacc accacccggg ataatacctt
      541 cttcgatggc tgcacgagta gcatgtaatg catcatcaac gcggtctttc ttttccttca
      601 tttcaacttc cgatggagct cccacataca acacagccac tccgcctgcc aatttggcca
      661 aacgttcctg caatttttcg cgatcgtaat cggaagtagt gttggcaatc tgcgatttta
      721 tctgactcac gcgtgctgca atagcttctt ttgaaccggc tccgtttaca atagtagtat
      781 tgtccttgtc aaccgtaact ttttcggctg ttccgagcat gtccatagta gctttttcaa
      841 gctgcaaacc tttctcttcg ctaattacaa caccattggt aagaattgcg atatcttcga
      901 gcatttcttt ccggcgatca ccaaatcctg gagcttttac ggcgcaaact ttcaaagaac
      961 cacgcaaacg gtttacaacc aacgttgtaa gtgcttcact gtcgacatct tcggcaataa
     1021 tcaataacga acgacctgat tgtgccacag gctcgagaat aggaagtaat tctttgaaat
     1081 tggatatttt tttgtcgtaa agcaaaatat atggtttttc catttcaacc aacatttttt
     1141 cggtattggt aacaaagtaa gccgaaatat aaccccgatc gaattgcatt ccttccacca
     1201 gttctatggt agtttcggtt cctttggctt cttcaatggt aataacacca tctttggaga
     1261 ctttacgcat ggcatcggca atcagtttac cgatttcgga gtcgttgttg gccgaaacgg
     1321 tagcaacttg ttcaattttg ttgtaatcgt cgtctacttt tttcgattga gaagcaatat
     1381 gttttacaac ttgagacacg gctttatcga ttccgcgttt caaatccatt ggattggccc
     1441 cggctgtaac gtttttcagt ccaacactta caatggcttg agccaacact gtagctgttg
     1501 tagttccatc accggcatca tcgcttgttt tggaagctac ttcctttaaa agctgagcgc
     1561 ccaggttttt gaatggatct tccagctcaa tttcttttgc taccgtaaca ccatctttag
     1621 ttatatgagg tgcaccaaat tttttttcga ttatgacatt tcttccttta ggtcccaatg
     1681 ttacttttac tgcattagcc aattcatcaa cacctttctt gagttgctca cgagcatcgt
     1741 ggttatataa aatttcttta gccatattct ttatgtttta atttaataaa cgttttttta
     1801 aatttatatt taagttatta attatcaagc tttcttgctt aatctattat tcccataata
     1861 tccgactggc gcataataag atatttttct ccatcccact ccagttcggt acctgcatat
     1921 ttgccatata acacagtatc tcccacagca acaatcattt cctcatcttt tgttcctttc
     1981 ccaacagcta ccacttcacc tttcagtggt ttttctttag ctgaatcggg aataataatt
     2041 ccacttgaag ttttttcttc tgccggagca ggttttacca acacacggtc tgctaaaggt
     2101 ttaatattca tatcacacta atttttaaat ggttaataaa taaatttata ttttttgtcg
     2161 tatttttcga tacaaataaa taaattcgct actgcctgaa tcaaaatgtg tgcctgataa
     2221 aaaaatagat gatattgaca tttttgacag aaagatgatg gaagaaaaaa attactaata
     2281 ctgtcatctt gtcattttct gagaatagtt acaaaaataa agaagcttgt aaaaatcgaa
     2341 taaaatcagg gaaggatttg aagaacaaag atttgcataa atagattttc gaaaaattcg
     2401 atatagaaaa gaaaagaatt cgatgagcga caattttttc aatgtcagaa acgaattcag
     2461 caaactggac tctttggcca gaaaaggata gcgaccggtt ttatacaaat aataaagatt
     2521 tcgaattcct tcttcagttt taaacaaaaa ggttggattg tcatccaatc ctttatgaat
     2581 caacggattg tcaatatgaa aaacattaaa acccaattca tgaatccgat gtccgaaaag
     2641 cgtgtcttcg tgcccgtagc cggaaatggt ttcatcaaag cgaattctgt tgaaaattga
     2701 ttttgcaatt aaaaaattaa atgccgaaaa atttgatttc ccgctcaatt cgagtttgtt
     2761 gcgtaaagtt gccgggcggg cttcgcgcaa tctgccatac tttaatcgca aactataggc
     2821 aaaatcatta aaattttcgt cataggctgt accacccacc accacagact cctctcgaca
     2881 aaaggaaacg tatttttcga caaaataggc ggaacaaact tcggcatcac aatcgagaaa
     2941 aagcaaatgc ccatattttg acatgtcagc caagcgattt ctgatagctg aacggccgat
     3001 atttttttct aaaacgatat aactgcaacg actcaattta tcgatttgcc tgttctcttc
     3061 caagtattca ttagaaccgt cttccattac aatgatctca aaatcaataa aaagttggag
     3121 agcctgccgg tgtaattcac caacaagacg ggaaactgaa taattgaata ccggaatcag
     3181 gattgaaagc attataaaag aaattgaaaa cttatcagaa atttttaacg ctcacaaaat
     3241 taattaaaaa tcaactgtat tcaaaaaatt tgattcgata acaaattttt tgacttcgcc
     3301 tctgataaaa tggaatttga atatttaaca aaaagcaaaa ttgaagttga ggaaggtaaa
     3361 ttttaattgt tacgatacct ttctataatt tacacaaaag ccaatttaac agaaaaagaa
     3421 actgtttggg cacgcttttg caattttcga gtcatttgtt ttaatatttc tatatcctaa
     3481 taaagtttat atgcagtgat cgggttttta taaatcacta cggcgtgtct tgccattgta
     3541 gcaatgatat cctcactaaa aaatgacagc ttctacattg tttgggtagt tcagtttcaa
     3601 aagcgaattt tttacctgtt caagattgaa taacaatgta tgcaattttt gcaaactgac
     3661 tatgtaagac ttagaattga agaccgggat gaagtatata gagaagtttt atctcctccg
     3721 acagttaaaa tttagtttat cattaattgt tcatcaaaag catcttcaga aagattatag
     3781 aagaattttc aaaaaaagca acggccagat taggacaaac aaaaccgatt gaataatttt
     3841 gaaatgtttt tttcgaaaaa taaagccgac taccataccg gccaacatac aacctaatac
     3901 agtaaacatt tgaaaatcaa taaaaatcaa cagtttcaaa caagaaaaaa tttcccatca
     3961 atatgggaat aactcagtat ttttcggtta aaaaaagaaa aaatcaaaac taaaaattta
     4021 cctgcaatga taatcccatt gtatgttgat gattccatga aaagtcaagt ttatttttgt
     4081 cgatataagg tgtaagttgt aatttaacct gttcctggct gtgcaaatcc ctttgataca
     4141 aattattgat tttggcaacg tgaaaagcat caacaacaga ccagatatcg attacaaaaa
     4201 gactaacaac accgctcaga gctaaaaagg gataaaaatc agcatccgac ctataataat
     4261 agtcattata atatccacta gagccaagtg caccaatcat gaaaacaaaa aaactcgctg
     4321 cagctccacc cataaaagca aaaccccttc caacttctcc gcaagttatt tgtcccaaac
     4381 ctggaatcaa ataagaagct atccctgcaa cccaaggctg ataaggatct ccgaatttag
     4441 gaaaatacaa ttgatcttta tagtaatttt ctttttcctt gtaagtttta aaactgatgg
     4501 gaggtgtcca tgaatcttga gttaaattac ggatccttcc gcgagatact gtaatacttc
     4561 ctttctcttc ttttatttcc gaaacatcag gtagattata aatttgcacc acaccgtttg
     4621 aaaaatgaat ttcttgtatc atcttaagtt cgtacacata atcgggacca tccaaatacg
     4681 aaaaatcctt acacgtcagt tctcccgctt ttatttgtgt gattttgcac gctttgattt
     4741 ctcctttttt gaaaacgatg gtatcttgag ccgtcaatag actaaatgct ccggcccagc
     4801 agaaaagtac aataaaaaaa tgttttttca tgttattgtt atattattaa tgatgcaata
     4861 atttttttat ataatttcta ataattaatt gcacataata ttttcaaagt ctcattcggc
     4921 t
//
LOCUS       DAOTAX010000100         4879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14411_length_4879_cov_30.058250, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000100 DAOTAX010000000
VERSION     DAOTAX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14411_length_4879_cov_30.058250"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1521
                     /locus_tag="PLM04_08075"
     CDS             334..1521
                     /locus_tag="PLM04_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HON03064.1"
                     /translation="MSLLSTVMGQYDEPQKVKSLGIYPYFRPIQSEQDTVVTINGKPV
                     LMFGSNSYLGLTNHPKLKEAAIRAIEKYGTGCAGSRFLNGTLDIHIELENRLAKFVHK
                     DAALVYSTGFSVNSGVIPCLTGRNDYLIFDERDHASIIEGKRLSFSKQFKFRHNDMDS
                     LEDVLKRCDYDKIKVIVVDGVFSMEGDIAKLPEIVELSKKYNASIYVDEAHTLGVFGK
                     TGAGICEHYGHTNDVDLIMGTFSKSLGTIGGFIASDNNIINYLKHNSRTLIFSASITP
                     ASAGCVLAALDVLEEETWRKEALWKNTARAKDGFMKAGFEIGHTESPIIPLYVRDNLK
                     TFQLTSLLMEEGVFVNPVVSPAVPSEDTLIRYSLMATHTFEQIDESIEKITKVARKLG
                     IIE"
     gene            complement(1723..2148)
                     /gene="ssb"
                     /locus_tag="PLM04_08080"
     CDS             complement(1723..2148)
                     /gene="ssb"
                     /locus_tag="PLM04_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HON03065.1"
                     /translation="MSVNKVILVGNVGKDPEVRYLEKNVAVARFPLATTERGYTMQNG
                     TQVPDRTEWHNIVAWRGLAEIVEKYVKKGSQLYIEGKIQSRSWEKDGIKRYTTEIYAE
                     NIQLLGKRSDTSEEPAAASTPPAETFQEGADIVEDDLPF"
     gene            complement(2292..2918)
                     /locus_tag="PLM04_08085"
     CDS             complement(2292..2918)
                     /locus_tag="PLM04_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HON03066.1"
                     /translation="MKKRIALSGCLLFFLAMNFFSQFSLQGGLNRASEIYVGSSASDA
                     NLPALKNLTGYNIGMMYQTPQKGFGFATGIALSQKGSIVNSDSLTDVYRQFNFLEIPL
                     HLRYCLDFGNMGLYGFGGVYGAYALSGQKISETPETQNNENFTYSDFNCRVDYGYTFG
                     AGIQFFKKIQIQATWNQGWKNLTNYLNGEKEPFSTKNKIFAVNLSYLF"
     gene            complement(2974..4791)
                     /gene="typA"
                     /locus_tag="PLM04_08090"
     CDS             complement(2974..4791)
                     /gene="typA"
                     /locus_tag="PLM04_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HON03067.1"
                     /translation="MQKIRNVAIIAHVDHGKTTLVDKMLLAGHLFRNNEKVGELIMDN
                     NELERERGITILAKNVSINYNGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIQMGLIPIVVVNKVDKPNCRPDEVQEAVFDLMYHLDANEEQLNFH
                     TIFGSAKQNWMSDDWKKPTDNILPLLDAIIKYIPAPPYLEGSPQMLITSMDYSPYVGR
                     IAVGRLHRGTLREGMEVSLVSRDGKVTKSRIKELHTFTGLGRTRTEEVKSGDICALVG
                     IEGFEIGDSIADALQPEPLKPIAVDEPTMSMVFTINNSPFYGKEGKFVTSRHIHERLM
                     RELEKDVALRVEKNPDSDIWHVYGRGVLHLSILIETMRREGYELQVGQPQVIFKEING
                     VRCEPVEQLSINLPEEYAGKIIEMVAARKGEMLSMEIVNDRVQLEFQIPSRGIIGLRN
                     NVLTASTGEAVMSHRFLEYQPYKGEIEKRNNGSLIAMESGTAFAYAIDKLQDRGRFFI
                     FPQEEVYAGQVVGESAKEGDIVVNVTKSKKLTNMRASGSDDKIKLTPPVVFSLEEALE
                     YIKEDEYVEVTPTKIRLRKIYLDENERKRMSKKSNVLSE"
BASE COUNT         1427 a          958 c          931 g         1563 t
ORIGIN      
        1 gactaaaaaa aataatccta atatcatatt gttctaatgg acaatttggg tttattttat
       61 aacctttgat ttgctgttaa gataatcaca ttagaccttt caaaaaaaat tcagcatact
      121 tttttaacta tgaaacccac aagtattgca tacacaaaca cgaatgaata taatatttaa
      181 tattcagaca tgtgggttcc atcagacctt aaaaaaataa attatattct gatgaaatat
      241 gaatctaaaa ttttctgttt ttaaaaagca ttaattatta atcaaaaaga tgtatctttg
      301 tgccgttttt aataaaaata taaactataa cttatgagtt tgttaagtac agtaatgggg
      361 cagtatgatg aacctcagaa agtaaaatca ttgggcattt atccttattt tcgtcctatt
      421 cagtcggaac aggatacagt tgttactata aacgggaaac ctgtattaat gtttggatcc
      481 aatagttatc tgggccttac caatcatccg aaactgaaag aagccgctat cagagcaata
      541 gaaaaatacg gcacaggttg tgcaggttcg cggtttctga atggaacact tgatattcat
      601 atcgaattgg aaaaccgttt ggcaaaattt gttcataagg atgctgcttt ggtatattcc
      661 actggttttt cggtaaattc gggtgtaatt ccatgtttga cgggacgaaa cgattatctg
      721 atttttgacg aaagagatca cgcttctatt attgagggaa agcgtctttc gttttcaaaa
      781 cagtttaaat tccgtcacaa cgacatggat tcgctggaag atgtgctaaa acgttgcgat
      841 tatgacaaaa taaaggtgat tgtagtggat ggcgttttca gtatggaagg cgacattgcc
      901 aaattgcctg agattgtgga attatccaaa aaatacaatg cttccattta tgttgatgaa
      961 gctcatactc tgggcgtttt cggaaaaaca ggagcaggaa tttgcgagca ttacgggcat
     1021 accaatgatg ttgatctgat tatgggaact ttcagcaaat cgttgggaac cattggcggt
     1081 tttatcgctt ccgacaacaa tattatcaat tatttgaaac acaattcgag aacattgata
     1141 ttcagcgctt ccattacacc tgcttctgca ggttgcgtgc tggcagcttt ggatgtattg
     1201 gaagaagaaa cgtggagaaa agaagcgttg tggaaaaaca cagcccgcgc gaaagatgga
     1261 tttatgaagg ccggttttga aattgggcac actgaatcac ccatcattcc tttatatgtg
     1321 cgggataatc ttaagacttt tcaacttacc agtttgctga tggaagaagg agtttttgtt
     1381 aatcctgttg tttcgccggc tgttccctcc gaagatacac tgatcagata ttcgttgatg
     1441 gctacccata cgtttgaaca gattgacgaa tcaatagaaa aaattacaaa agttgcccgc
     1501 aagttgggaa ttatagaata attacacgaa agttttttca ttcaattgtc aacagaatgt
     1561 gtcaaattat tgatacattc tgttttgctt ttaaagaaat agactgacga taaccattta
     1621 tatattttgt gcaagtttta aaatatggta ggataaaaac aggtagaaca aaaaaagcac
     1681 acaacaatag cgtcatgtgc tttggagaaa ataagaattt tttcagaaag gcaaatcgtc
     1741 ttctactata tcagcaccct cctgaaaggt ttctgcaggg ggagtagaag ctgcagcagg
     1801 ttcttcagat gtgtccgaac gttttcctaa cagttggata ttttcagcat aaatttccgt
     1861 agtgtaacgt ttaattccat ctttctccca tgagcgactt tgaatttttc cctcgatgta
     1921 aagctgactt ccttttttta cgtatttttc tacaatttct gccagccccc tccaagctac
     1981 aatattgtgc cattcagttc gatcgggaac ttgcgttccg ttttgcatgg tataaccccg
     2041 ttcagtggta gccaaaggaa aacgggctac tgcaacattt ttttccaagt aacgcacttc
     2101 gggatctttt cctacattcc ctactaaaat aactttatta acagacataa tttcaatgat
     2161 taaaatggtt tgaattgagt tgaactgccc gtaaaaatac attttttttt atacgtatca
     2221 aatttttttc aaaaattttg aaaaaaatga gctcgattct ctcgtcgatg attattaaat
     2281 agaatggttg tttaaaacaa atatgacaaa ttgacggcaa atattttgtt tttagtagaa
     2341 aaaggttcct tctccccatt caaataattg gttagatttt tccatccctg attccaagta
     2401 gcctgaatct gaattttttt gaagaattga attccggctc caaatgtgta gccatagtcg
     2461 actcgacaat taaaatcgga ataagtaaaa ttttcgttgt tttgggtttc aggtgtttca
     2521 gaaatttttt gtccactcaa ggcataggcg ccgtaaactc ctccaaatcc atacagcccc
     2581 atatttccaa aatccagaca atagcgcaaa tgcagaggga tttccagaaa attgaattgg
     2641 cggtaaacat ctgtcaagct gtcggaattt acaatactgc ctttctgaga aagggcaata
     2701 cctgtggcaa aaccaaagcc tttttgtgga gtttggtaca tcatcccaat gttgtatccc
     2761 gtcaggtttt ttaaagccgg caggttggca tcagaagcac tactgccgac gtaaatttct
     2821 gaagctctgt tgaggccgcc ttgtagggag aattgcgaaa aaaaattcat tgcaaggaaa
     2881 aataacaaac atcccgacaa ggctattctt tttttcataa attcttgtgt accgctcttt
     2941 tttaatggaa tcgttttgtt ccgaaataaa ttcttattca ctgaggacat tcgacttttt
     3001 cgacatgcgt ttacgttcat tttcatcaag atagattttc cttaagcgaa ttttggttgg
     3061 agtaacttcc acatattcgt cttctttgat atattccaaa gcttcttcga gactgaaaac
     3121 taccggtggt gtaagtttaa ttttgtcatc agaacctgat gcccgcatgt tggttaattt
     3181 tttcgacttg gtaacattca ccacaatgtc gccttcctta gcgctttcac ccacaacctg
     3241 accggcataa acttcctcct ggggaaaaat gaaaaatctg cctctgtcct gtaatttgtc
     3301 gatggcataa gcaaatgccg tgccactttc cattgcaata agcgatccat tgtttctttt
     3361 ttcgatttca cccttgtaag gttggtattc caaaaatcgg tgcgacataa cggcttctcc
     3421 tgttgaagct gttagtacat tattccttaa tccgataata cctcgtgaag gaatttgaaa
     3481 ctccagctgt acacggtcgt ttactatttc catgctcagc atttcgcctt tgcgggctgc
     3541 aaccatctca atgatttttc cggcatattc ttcagggaga ttgatgctca actgttcgac
     3601 cggttcgcaa cgaacgccgt ttatttcttt gaaaattacc tgtggctgtc ctacctgaag
     3661 ttcgtaacct tctctgcgca tggtttctat cagaatggaa aggtgtaaaa cacctcttcc
     3721 atagacgtgc caaatatccg agtctggatt tttctccacg cgtaaagcga cgtctttttc
     3781 caattctcgc atcaatcgtt catgaatgtg tcgtgaagta acgaattttc cttcttttcc
     3841 gtaaaaaggc gaattattga tggtgaaaac catactcatg gtaggctcat ccactgcaat
     3901 gggtttcaac ggttccggct ggagtgcgtc ggcaatggaa tcgccaattt caaaaccctc
     3961 tattcccacc agcgcacaaa tatcacccga tttcacttct tccgtgcgcg ttcttccaag
     4021 tccggtaaaa gtatgcaatt ccttaatgcg ggattttgta accttgccat cacgacttac
     4081 caggcttact tccattcctt cgcgaagtgt tccacgatga agtctgccaa cggcaattct
     4141 tcccacatac ggagaataat ccatcgaagt gataagcatt tggggagagc cttccaaata
     4201 aggcggagca ggaatatact tgataatggc atccagcaag ggtaaaatat tgtcggttgg
     4261 ttttttccaa tcgtccgaca tccaattctg tttggctgat ccaaaaatag tgtgaaaatt
     4321 caactgttct tcattggcat ccaaatgata cattaagtcg aagacggctt cctgaacttc
     4381 atcgggacgg caattgggct tgtcgacctt attgacgacc acaatgggaa tcagtcccat
     4441 ttgaatggct ttttggagaa caaaacgggt ttgtggcatc ggaccttcaa aagcatctac
     4501 caaaagtaac actccatcag ccatatttaa aaccctttct acttctcctc cgaaatcggc
     4561 gtgtcccggt gtgtctataa tgttgatttt atatccgttg taattgatgg aaacattttt
     4621 agccaatatg gtgattcctc tttcgcgttc gagttcatta ttgtccataa tgagttcacc
     4681 aactttttcg ttgttgcgga aaaggtgtcc tgccagtaac atcttgtcta ccaatgtagt
     4741 tttcccgtgg tcgacatggg caatgatggc tacatttcta attttttgca taagttaaag
     4801 agctaatttt ttagcctgca aagatagcca aaaaaattga atttttattg cccgtttttt
     4861 tgaatttagc taaagcaaa
//
LOCUS       DAOTAX010000101         4828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14592_length_4828_cov_31.662057, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000101 DAOTAX010000000
VERSION     DAOTAX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14592_length_4828_cov_31.662057"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..218)
                     /locus_tag="PLM04_08095"
     tRNA            complement(145..218)
                     /locus_tag="PLM04_08095"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Ala,seq:ggc)
     gene            305..619
                     /locus_tag="PLM04_08100"
     CDS             305..619
                     /locus_tag="PLM04_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HON03068.1"
                     /translation="MLIFNTTYLVENEFYPQWINWVQESLIPFMMSTAGFSEPQIAKV
                     LSGESVKETSYSVQFKISDKNALNDWMRQYGEDFAKLCSANFGNHVLFFSTMLELISD
                     DK"
     gene            609..1193
                     /gene="ruvC"
                     /locus_tag="PLM04_08105"
     CDS             609..1193
                     /gene="ruvC"
                     /locus_tag="PLM04_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HON03069.1"
                     /translation="MINDQIILGIDPGTTIMGYGVLNIRAHQPELMAMGILDLKKYAN
                     HYVKLQRIFARTLSLIDEFHPACMAIEAPFFGKNVQSMLKLGRAQGVAMSAALYRDIP
                     ITEYAPLKIKMAITGNGNASKEQVADMLRRFLHIPQENMFPQLDATDGLAAAYCHFLQ
                     LGKPESQKVYSGWKDYISKNQEKVKNPIQKKREK"
     gene            1284..2384
                     /locus_tag="PLM04_08110"
     CDS             1284..2384
                     /locus_tag="PLM04_08110"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HON03070.1"
                     /translation="MKKSFLLVLCLLFLFQSGEGKIYRFLVGTYTQNTVSEGIYSLSF
                     DAESGVCKIQLAATNVDNPSFLTFSPDKNFVYSVNESGTENKIASFRFDATSGKLFFI
                     NRVEANGSDPCFITASKQHVITANYSSGNACVFKRNSDGSIGETVQKIQFNGHSIDSM
                     RQTSSHVHQTIFSPNGHFLLINDLGTDLIHSFRYVPNRSENCLLPYDSLKVKPGSGPR
                     HLTFSKDGKFVYLLHELDGTISIIGFNKGKLLFYGENSVVTNKKLKTGAADIHISPDG
                     KFLYATNRGEANNITCFKILKNGLLEFVEQNSTQGKGPRNFAITKDGHFLLVGHQYSD
                     EIVVFKRDRENGKLMDTKIRIKIGAPVCLLEY"
     gene            complement(2394..4667)
                     /locus_tag="PLM04_08115"
     CDS             complement(2394..4667)
                     /locus_tag="PLM04_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HON03071.1"
                     /translation="MADEKSIQEQEREEERMIQQEFEGLLEDYRNSNHRQKVEIITKA
                     FNFANTAHKGMKRRSGEPYIMHPLAVARIVVKEIGLGSTSICAALLHDVVEDTEYTVE
                     DIRHHFGDKIAQIVEGLTKISGGVFAEKASEQAENFRKLLLTMSDDIRVILIKIADRL
                     HNMRTLGSLPPAKQYKISGETQYIYAPLAHRLGLFRIKTELENLSFKYEHPDIYKSIE
                     NKLLMDEEARNAFYKEFSAPIREKLTEMGYEFTLKERVKSVYSIWHKMSTRNIPFEEV
                     YDILALRIIFKPKEGMPEKDQCWMIYSAITALYKPHPERIRDWLSTPKANGYEALHVT
                     VMGPNGRWVEVQIRTERMNEIAEKGLAAHWKYKLGENDESELDNWLKTIKEVLEHPDT
                     DAINFLETFKLTLFASEIFVFTPKGEIKTIAQGATALDFAFMIHSDLGMHCIGAKVNH
                     KLVPLNHRLQSGDQVEILTSQKQTPKEEWLNYVTTAKARSKILELLKKEEKNIAAEGQ
                     KMIENALAEINLPASNENIMKILQHYNLTQRNELYVQAGKGALNLDDIKKVIFKPKTQ
                     NVFSKYLKIPFIGSSSSENKEIIIPTQQIDRKATLKLTEENTGKIFKLAECCHPIPGD
                     DVLGYLDENGIIYIHKRQCPVALKLKSNFGERILSAEWATHKILSFEETIEIKGIDRK
                     GILIEILKVISEAYGVNISKINIETESGIFIGKFQVLVHDTEELNNMCENLTKIPEVQ
                     SVQRIEQTNLQPQEQKT"
BASE COUNT         1384 a          857 c          979 g         1608 t
ORIGIN      
        1 caaatgtatt tagtaattga cgacattggt ctgcaaaagg taaaaaaaaa taagcaaaag
       61 gtaaaaaagt gataaatatt tcttttttca atggatggaa aggaggaaaa ataaaaaaag
      121 cttaccaaag ataagctttt cgagtggaga ttaccggagt cgaaccggtt acctcgacac
      181 tgccagtgtc gcgctctagc caaatgagct aaacccccaa aatgtatatt ttttcactgc
      241 aaagttaaag aactttttca aattatgctt acttttgttc aaattattcc ataaaaaatt
      301 acaaatgctg atttttaata caacttattt ggttgaaaac gaattttatc ctcagtggat
      361 aaattgggtt caagaatcat tgattccatt tatgatgagc actgccggtt tctcggagcc
      421 tcaaattgcc aaagtgcttt ccggtgaaag cgtgaaggag acttcctatt cggttcagtt
      481 taaaatttcc gataaaaatg ctttaaacga ttggatgagg caatacggag aagattttgc
      541 aaaattatgt tcagctaact ttggaaacca tgttcttttc ttttcaacca tgctcgaatt
      601 gattagtgat gataaatgac cagattatat tgggtattga tccgggaact accattatgg
      661 gatatggagt gctgaatatc agggctcatc aacccgaatt aatggctatg ggaattcttg
      721 atttaaaaaa gtatgccaat cattatgtaa aattacaacg tatttttgct cgaacattgt
      781 cgcttataga tgaatttcat ccggcctgca tggccataga agctcctttt ttcgggaaaa
      841 atgtgcagtc gatgctgaaa cttggaaggg cgcagggcgt agccatgtcg gcagctctgt
      901 atcgtgatat tcccattacg gaatatgcac cgttgaaaat aaagatggct ataacgggca
      961 acggaaatgc ttcaaaagaa caggttgccg atatgttgcg caggtttttg catattcctc
     1021 aggaaaacat gtttccacag ctggatgcta ctgatggctt ggcagcagcc tactgtcatt
     1081 ttttacagtt gggaaaaccc gaatctcaga aagtttattc cggctggaaa gattatattt
     1141 caaaaaatca ggaaaaagtg aaaaatccca tacagaaaaa aagagaaaaa tagatttttc
     1201 agtcaagaaa gaaacgttta gagctttcaa acagatttat ataacatgtt gattaacaat
     1261 atactgattt ataaatattc ataatgaaaa agtcattttt attggttttg tgtttgttgt
     1321 tcctttttca gtcgggagaa ggaaaaatat atcgtttttt ggttggaact tatacgcaga
     1381 atactgtgag cgaaggaatt tattctttaa gttttgatgc tgaaagtgga gtttgtaaga
     1441 ttcaacttgc tgcaactaat gtggataatc caagtttttt gacattttcg cccgacaaga
     1501 attttgttta ttccgtcaat gagtcgggta ctgagaataa aatcgcatct tttcgttttg
     1561 atgccacttc aggcaaactg ttttttatta accgtgtgga agcaaatggc tcggatccct
     1621 gttttattac tgcttcaaaa caacatgtta tcacagccaa ttattcaagt ggcaatgctt
     1681 gcgtttttaa gagaaactca gatggttcga taggtgaaac ggttcaaaaa attcagttca
     1741 atggacacag cattgattcc atgcgtcaga caagttctca tgtacatcaa acgatatttt
     1801 caccaaacgg acatttttta ttgatcaacg atttgggaac cgatttgata cattcttttc
     1861 gttatgttcc caatagatca gagaattgtc ttctacctta tgacagttta aaagttaagc
     1921 cgggtagtgg acctcgacat ttgactttct ccaaagacgg aaaatttgtt tatttgcttc
     1981 acgaattgga tggaacaatt tccattatcg gatttaataa aggaaaattg ttgttttatg
     2041 gcgaaaattc tgtagtaacg aataaaaaat tgaaaaccgg agcggccgac attcatattt
     2101 ctcccgatgg taagtttctg tatgccacca atcgtggaga agccaacaat attacctgtt
     2161 ttaaaatact gaaaaacggt ttgttggaat ttgttgagca aaattcaact cagggcaaag
     2221 gaccgcgtaa ttttgcaatt accaaagacg gccatttttt gttggttggc catcagtatt
     2281 ccgacgaaat tgtggttttt aaaagggaca gggaaaacgg gaaactgatg gatacgaaaa
     2341 tacggataaa aattggagcg cctgtttgcc tgctcgaata ttgagaggtt ttattaggtt
     2401 ttttgttcct gaggttgcag gttggtttgt tcgattcgtt gaacagactg aacttcggga
     2461 attttggtca gattttcgca catgttgttg agttcttcgg tgtcgtgtac caacacttga
     2521 aatttgccga taaaaattcc actttccgtt tcgatattta ttttgctgat gttgacgccg
     2581 taagcttcag aaattacttt taaaatttcg atcagaattc ctttgcggtc aattcctttt
     2641 atttcgatag tttcttcgaa agatagaatt ttatgtgtgg cccattcggc agaaagaatt
     2701 ctttcgccaa agttactttt taatttcaat gccaccggac attgtcgttt gtgaatgtag
     2761 ataatgccgt tttcgtcgag atagcccaac acatcatcac ccggaatggg atgacagcat
     2821 tctgccagtt tgaatatttt tcccgtgttt tcttcggtca gtttcagggt agcctttctg
     2881 tctatttgct gagtgggaat aataatttcc ttgttttcgg aagaagagct tccaataaag
     2941 ggaatcttta aatatttgga aaaaacattt tgcgtttttg gcttaaagat aactttttta
     3001 atatcgtcaa ggtttagagc tcctttacct gcctgaacat aaagttcatt tcgttgggta
     3061 aggttgtaat gctgaagaat tttcatgata ttttcgttgc tggcgggcag gtttatttcg
     3121 gccaacgcat tttctatcat tttttgacct tccgccgcaa tgtttttttc ttcttttttc
     3181 aggagttcga gaattttgct tctcgctttg gctgtagtaa cgtaattcaa ccattcttcc
     3241 ttaggagttt gtttctgcga agttaggatt tcaacctgat cgccgctctg caaacggtgg
     3301 ttcaagggca caagtttgtg attcactttg gctccaatgc agtgcatacc caaatcagaa
     3361 tgaatcataa aggcaaagtc gagagcggta gctccctgag caatggtttt gatttctcct
     3421 tttggtgtaa aaacaaaaat ttcggatgca aataaggtta atttgaaagt ttcgagaaaa
     3481 tttatggcat ccgtatccgg gtgttcaagt acttctttaa tggttttcag ccaattgtcc
     3541 aattcagatt catcgttttc tcccagtttg tatttccagt gggcagccaa ccctttttcg
     3601 gcgatttcgt tcattctttc cgtacgaatt tgtacttcca cccaacgacc gtttggcccc
     3661 attaccgtta cgtgaagtgc ttcatatccg ttggctttgg gtgtactcag ccagtctctg
     3721 atgcgctcgg gatggggttt gtaaagtgca gtaatggcgg aataaatcat ccagcactga
     3781 tctttttcag gcataccttc tttgggcttg aaaataatgc gaagtgccag aatatcatat
     3841 acttcttcga aagggatatt gcgggttgac attttgtgcc aaatggaata gactgatttt
     3901 acacgttctt tcagggtaaa ttcatatccc atttcagtca atttttccct gataggagca
     3961 gaaaattcct tatagaaagc atttcttgct tcttcgtcca tcaacagctt gttttcgatg
     4021 gatttgtaaa tatctggatg ttcgtatttg aaactgagat tttcgagttc ggttttaatg
     4081 cgaaaaaggc caagtctgtg ggcgagtgga gcataaatgt attgggtttc gcccgaaatt
     4141 ttgtattgtt tggccggagg aagtgatccc aaagtgcgca tattgtgcag tctgtcggca
     4201 attttaatca ggatgacgcg aatatcgtcc gacatggtga gaagaagttt tctgaaattt
     4261 tcggcttgtt cagaagcttt ttcggcaaaa acacccccgg aaattttggt aagtccttct
     4321 acaatttgtg caattttgtc accaaaatga tggcgaatat cttcaacagt atattcggta
     4381 tcttctacca catcgtgcaa caaggcggca caaatggaag tagagcccaa gcctatttct
     4441 tttactacaa ttcgtgccac agccagtgga tgcatgatgt atggctcgcc ggaacgtcgt
     4501 ttcatgcctt tatgagcagt attggcaaaa ttaaaggctt ttgtaatgat ttcgactttc
     4561 tgccggtgat tggaatttcg atagtcttcg agcaaaccct caaattcctg ttgaatcatt
     4621 ctttcttctt ctctttcttg ttcttgaata ctcttttcgt cggccataaa cttgcatcct
     4681 attttatgca aatataaata atttattcaa tttcgatttg tttttttaca acaaaaactc
     4741 atttcaatcc attattgaaa acaattttca gattgaaaga gttcgacaaa gtcaatcaga
     4801 atgaatttta ttctaataac ctgctaac
//
LOCUS       DAOTAX010000102         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14599_length_4826_cov_39.543911, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000102 DAOTAX010000000
VERSION     DAOTAX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14599_length_4826_cov_39.543911"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2367
                     /locus_tag="PLM04_08120"
     CDS             <1..2367
                     /locus_tag="PLM04_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HON03072.1"
                     /translation="KTNDIEVVIIDEAHRFRNEDTKDYEYLKNICRNKIVILLTATPF
                     NNKPADIFSLLKLFIVPKKSSITLDDDLENKFRTFKSEFDKLSYIKRYHQSSDRQKQN
                     RALSYYNDLFGDEKIVDSTKIKKRTEILAKQIRDVIEPVIIRRNRLDIQNHPYYKNEV
                     NELSIVESPKEWFYELNKEQSVFYDKIIQQYFALPDDGGRFKGAIYKPFEYELQKVKK
                     GELGKEENFQYLQQFNLYDFMRRLLVKRFESSFGAFEQSVKNFINIHQIVLEFIQKTD
                     KYILDRNLLEKIYELDADEIENYLQEYSEKIKNNVYPKNHKVYELKKFERQTEFIQDI
                     QSDIALFQQILNELHKLNLVAKDPKAECLIEEVKTILDAPPENLHEPKRKVIIFSEYT
                     DTVNYLAPLLTQVFNARVLVVAGDLSASKIKEINKNFDASATQQADDYDILLTTDKIS
                     EGFNLNRAGMVINYDVPWNPVRVIQRVGRINRISRKVFDKLYIINFFPTEKGSDLVHS
                     REIAAQKMFMIHNTLGEDAKIFDIDEEPTPSGLYNKILQSPDAIEGESSYTKISKEFE
                     AWKEKYPDMVASLKNFPSRIKVAKDGAENELFVVIKKQKLFVHQQLYDAKEGSENHFI
                     KEVEDILEKIKADINEPPLPLSNQFWEEYEKIKNYQENPKNYKPIPDNNLQTRAINVL
                     RNFLKINDNQSVTILKPFVRMLLEDLLDYGTLSDYTIRRIANIDIDKNFTKAVDELKS
                     IEKELGSDYLYKEKQRISDLSKEIIIAIENRKTNELPTQNTFNDNISK"
     gene            2375..2566
                     /locus_tag="PLM04_08125"
     CDS             2375..2566
                     /locus_tag="PLM04_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03073.1"
                     /translation="MEFFVQFNKWIIMPNPIHGIIIINKNNAAKQINKLQPSKNQLIW
                     QRNDAEHIIRNDNELNRIR"
     gene            2635..>4826
                     /locus_tag="PLM04_08130"
     CDS             2635..>4826
                     /locus_tag="PLM04_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03074.1"
                     /translation="MDDKKEILTILEKIINDFSIDNLVRLFQNKTTKFRMLQSPASQP
                     LFTDGLLLGEFGCGDESVCIFTFKTHQPLTERSGKKAQYELAKKMLKEQIRYDGGFFI
                     FYDEQGNFRFSLIYDIPLSSGTREWSNFKRYTYYVSKEQTNKTFIQQMAEADFSSLNS
                     IIKAFSVEKVTREFYNDIANWYFWALQKVQFPKDAEQQSNGRNIALIRFITRIIFVWF
                     MKEKKLISPQLFDFDHIKNILKSTDDNESTYYKAILQNLFFATLNTPIPERKFRTENR
                     FQGKNEDYMNHEYFRYQELFSDPKQDINLLFNEIPFLNGGLFECLDKPKNDPSNDTGD
                     EIRMDGFSDKKENQPIFPNELFFSKEKNVDLTEYYGDTKYKNTRVQGLISILNAYNFT
                     VDENTPLDEEVALDPELLGKVFENLLASYNPETATTARKATGSYYTPREIVDYMVTES
                     LKYYFKTKFENTFPPEELDEKLTSLFSYSHEEQPFNEKETDFIIEKINELKIIDPAVG
                     SGAFPIGVLNKLVFLLSKLDPHNEKWKAQQFRALDYITDPTLKNELREKIENNFKRNE
                     LDYGRKLYLIQNCIYGVDIQPIAIHIAKLRFFISLLVDEKINPNIDDNFGIEPLPNLE
                     TKLVAANTLIGLPTSKQLVIKSDETKQIEAELFQIRKQHFTANYEKKKKEIQKRDREL
                     RNRLKELLEESGFPSDYTEKIASWDLYDTNRSAEWFDPEWMFGVKNGF"
BASE COUNT         1773 a          940 c          821 g         1292 t
ORIGIN      
        1 aaaaccaacg atatcgaagt ggtaattatt gacgaagccc atcgattcag aaatgaagat
       61 accaaagatt acgagtacct gaaaaatatt tgtcgcaaca aaatcgttat cttgctcact
      121 gctactcctt tcaacaacaa accagccgat atattttcgc tgctcaaact ctttattgtt
      181 cccaaaaaat caagtattac ccttgatgat gatttggaaa acaaatttag aacattcaaa
      241 tcggaatttg ataaactttc ctacatcaag cgatatcacc aatcgtctga ccggcaaaag
      301 caaaacagag ccctttctta ttataatgat ttatttggcg acgaaaagat agtcgattcg
      361 actaaaataa aaaaacgaac agaaattctg gcaaaacaaa ttcgcgatgt tattgagccg
      421 gtgattattc gccgaaatcg gctggacatt caaaatcatc cctactacaa aaatgaagta
      481 aatgagctct caattgtaga atctccaaaa gaatggttct atgaactgaa caaggaacaa
      541 tcggtatttt acgacaaaat cattcaacaa tactttgcct tgcctgatga cggaggacgt
      601 tttaaaggag ccatttacaa accttttgaa tatgagttgc aaaaagtaaa aaaaggtgag
      661 ctgggcaaag aagaaaattt ccaatatttg caacaattca acttgtacga ttttatgcgg
      721 cgattgctgg taaaacgttt cgaaagttca tttggcgctt ttgaacaaag cgtgaaaaac
      781 ttcatcaaca tccatcaaat tgtactggaa tttatccaaa aaaccgataa atatattctc
      841 gacaggaatt tgctggaaaa aatttatgaa ttggatgccg acgaaattga aaattatttg
      901 caagaatatt ctgaaaaaat caaaaataat gtatatccta aaaaccataa agtgtatgaa
      961 ctgaaaaaat ttgaacgtca aaccgaattt attcaggata ttcaatcaga tattgcatta
     1021 ttccagcaaa ttcttaacga attgcacaaa ctgaatctgg tagcaaaaga ccccaaagcc
     1081 gaatgcctca tcgaagaagt aaaaacaata ctcgacgctc cgcccgaaaa cctccatgaa
     1141 ccgaaaagaa aagttatcat tttttcggaa tataccgata cggtcaatta tctggctccc
     1201 ttgctcaccc aagttttcaa cgcacgtgta ctggtggttg ccggagattt gtcggcaagt
     1261 aaaatcaaag aaatcaataa aaatttcgat gcctccgcta ctcaacaggc tgacgattat
     1321 gatatcttat tgactaccga taaaatttcg gaaggattca atttgaatcg tgccggcatg
     1381 gtaatcaatt acgatgttcc ctggaatccg gtacgcgtga ttcagcgtgt aggaagaatc
     1441 aaccgcatca gcagaaaagt ttttgataaa ctttacatta tcaacttttt ccctaccgaa
     1501 aaaggttcgg atctggttca ttcaagagaa attgcggcac aaaaaatgtt catgattcac
     1561 aatacgctgg gcgaagatgc caaaattttc gatatcgacg aagaacctac gccatcagga
     1621 ctttataaca aaattctgca aagtccagat gcaatcgaag gcgaaagttc ctatacgaaa
     1681 atatcgaaag aatttgaagc atggaaagaa aaatatcctg acatggtggc ttcgctgaaa
     1741 aattttcctt cgcgcataaa agttgccaaa gatggtgcag aaaatgaatt gtttgtggtt
     1801 ataaaaaaac aaaagctttt tgttcatcaa cagctctatg atgcaaagga aggaagtgaa
     1861 aatcatttta ttaaagaagt ggaagatatt ctcgaaaaaa taaaagccga cataaacgaa
     1921 cctcctttac ccttgagtaa ccaattctgg gaagaatacg aaaagattaa aaattatcag
     1981 gaaaatccaa aaaactataa acctattcct gataataacc tgcaaacgcg tgctattaat
     2041 gtgttgcgta atttcttaaa aataaatgat aatcaatcgg ttaccatact gaaacctttt
     2101 gtaagaatgc tgctggaaga tttgctcgat tacggtacgc tttccgatta caccatcaga
     2161 agaattgcca atattgacat tgataaaaat ttcacaaaag cagttgatga actgaaatcc
     2221 attgaaaaag aattggggag cgattattta tacaaagaaa aacaacgtat ttccgattta
     2281 tcaaaagaaa tcatcattgc catcgaaaac agaaaaacga atgaattgcc aacacaaaat
     2341 acattcaatg acaacatttc aaaataaata ttgaatggaa tttttcgttc aattcaataa
     2401 atggattata atgccaaacc ctattcacgg catcatcatt atcaataaaa acaatgccgc
     2461 aaaacaaatc aataaattgc aaccatcaaa aaatcaattg atttggcaac ggaatgatgc
     2521 cgaacacatt attcgcaacg acaatgaatt aaatcgcatt cgttaatata tttttgagaa
     2581 tcccttgaaa atgggaaaac gataaaaatt tcataacaat aaaccaaaaa acgtatggat
     2641 gataaaaaag aaattctgac cattttagaa aaaatcatca acgatttttc aatcgataac
     2701 cttgttcgtt tgttccaaaa caaaaccacc aaatttcgca tgctgcaatc gcctgcatcc
     2761 caaccattat ttactgacgg tttattgttg ggagaatttg gttgcggcga tgaaagtgtt
     2821 tgtattttta ccttcaaaac ccatcaacct ttaaccgaac gctcgggcaa aaaggcacaa
     2881 tacgaattgg caaagaaaat gctgaaagag caaatacgtt atgatggcgg atttttcatt
     2941 ttttacgacg aacagggaaa ttttcgtttc tctttgattt atgacattcc tttatcgtcc
     3001 ggaacaagag aatggagcaa ttttaaacgc tacacctact acgtcagcaa agaacaaacc
     3061 aacaaaacct ttatccagca aatggccgaa gccgattttt cttcgctcaa ctccatcatc
     3121 aaggcattca gcgtagaaaa agttacccgt gaattctaca acgatattgc caactggtat
     3181 ttctgggcac tgcaaaaggt gcaatttcca aaggatgcgg agcagcaatc caatggaaga
     3241 aacattgctc ttattcgttt tattacccgc atcatttttg tatggtttat gaaggaaaag
     3301 aaattgatca gtcctcaact tttcgatttt gaccacatta aaaatatcct gaaaagcaca
     3361 gacgacaacg aaagtactta ctacaaagcc attcttcaaa atttattttt tgcgacactt
     3421 aatacgccca ttcccgaaag aaaatttaga acagaaaata gatttcaggg taaaaacgaa
     3481 gattacatga atcatgaata ttttcgttat caagaacttt tctctgatcc caaacaggat
     3541 atcaaccttc tgttcaacga aattcctttt ctcaacggcg gactgtttga atgtctggat
     3601 aagccaaaaa acgatccttc caatgatacg ggcgacgaaa ttcgcatgga tggattttcg
     3661 gataaaaaag aaaatcaacc catctttccc aacgaacttt tcttttcaaa agaaaaaaac
     3721 gtggacttga cggaatatta tggtgataca aaatataaaa ataccagagt acaagggtta
     3781 atttctattt taaatgccta caatttcacg gtagatgaaa atactcctct tgacgaagaa
     3841 gtggcactcg atcctgagtt gttgggaaaa gtattcgaaa acctgctggc ttcctacaat
     3901 cccgaaacag caaccacagc acgcaaagct accggaagtt attatacgcc acgcgaaatt
     3961 gtggattata tggtaaccga atcgctcaag tattatttta aaaccaagtt tgaaaacact
     4021 ttccctcccg aagagcttga cgagaaacta acttcacttt tttcatacag ccacgaagag
     4081 caacctttca acgaaaaaga aaccgatttc attattgaaa aaatcaatga gctcaaaatc
     4141 attgaccctg ctgtcggttc aggtgctttc cccattggcg ttttaaataa gctcgttttt
     4201 ctgctttcaa aactcgatcc gcataacgaa aaatggaaag cccaacaatt ccgggcactc
     4261 gactacatta ccgaccccac attaaaaaat gaacttcgcg aaaaaattga aaacaatttc
     4321 aaacgaaatg aactcgacta cggacgaaaa ctctatctga tacaaaattg tatttacgga
     4381 gtggatattc aacctattgc cattcacatt gccaaacttc gattttttat ttctttgctg
     4441 gtggacgaaa aaataaaccc gaatattgac gacaatttcg gcatcgaacc tttgcccaat
     4501 cttgaaacaa aattagtggc tgccaatacg ctcattggat tgcccacatc taaacaactg
     4561 gttattaaaa gtgatgaaac aaaacaaatt gaagcagaac tttttcagat tcggaagcaa
     4621 cattttacag ccaattacga aaagaaaaag aaagaaattc aaaaaagaga ccgcgaattg
     4681 agaaatcggc tgaaagaatt gctcgaagaa agtggtttcc cttccgatta taccgaaaaa
     4741 atagcctcgt gggatctata cgacaccaac cggtcggccg agtggttcga tcccgaatgg
     4801 atgtttgggg taaaaaacgg attcga
//
LOCUS       DAOTAX010000103         4807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14670_length_4807_cov_31.329756, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000103 DAOTAX010000000
VERSION     DAOTAX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14670_length_4807_cov_31.329756"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1870)
                     /gene="gldG"
                     /locus_tag="PLM04_08135"
     CDS             complement(182..1870)
                     /gene="gldG"
                     /locus_tag="PLM04_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HON03075.1"
                     /translation="MRNIIQHKQLFFWGVFLLLSLVLSFVHYRVDLTSEKRYSLSEPS
                     KKLMKKLDFPLKVKIYLDGSLNSGFLRLKKSTVEMLEELKVYSSKPIAIEFEDPSSVV
                     KPEEREKHYAELESRGLTPTNVYERDNDGKIIRKTIFPWLEMEYKGRKVLVNLLKNIP
                     GNSGDENLNISIIELEYEITDAVRRLVNTSVDKIAFLEGHDELSEAATYDISKSLSRY
                     FQIDRGRIGNDAPILHPYKVVIVAKPQLPFSEKEKLIIDQYIMNGGKVLWLIDGVRLA
                     EENFSVTGTSPAIPLDLNLSDLLFRYGIRIKPVILQDVQCAQMPVNVAKPGKAPHFEP
                     VPWFFAPLLLTSYAHPVTRNLATVRSEFCSAIDLVGENQQVSAVLLLATSDNTHVIGT
                     PTTVDLRDMPSANDKNYFNTGYVPVGVLLEGNFESVFTNRMLPDSVVNISSIRPVSVN
                     TKQIFVADGDIIKNEISIVQGDSIYLPLGFDRLTGLQYGNRDFIENAVLYLADDEGWM
                     QLKNKTLKLRLLNRQVVYDQKLKWQLVNVLLPLVILAIFGFVFQLIRKRKFTRS"
     gene            complement(1967..2851)
                     /locus_tag="PLM04_08140"
     CDS             complement(1967..2851)
                     /locus_tag="PLM04_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HON03076.1"
                     /translation="MKKKIAFIINPMAGVRTKSFIPQHIHDELNRDLFDPTVAFTDYR
                     GHGSLLARDFAAKGFDSVVAVGGDGTINEVARGLVQTSVSLGILPIGSGNGLARHLEI
                     PMNMRKAIQMLNFSEEISIDYGLVNQRPFFCTCGTGFDAYIAAEFAKGHRRGIVAYLE
                     KIVKGYFAYHSENYRIIGKNIDFTTKTFLMTVANASQWGNNVYIAPKASVQDGRLDVS
                     IMSNFPIIALPTLALQLFTKTISNDLFINTFRMSEITVFRPAPGPFHYDGEPYEEGKE
                     INIRVVPAGLKVLVKKRF"
     gene            complement(3294..4586)
                     /locus_tag="PLM04_08145"
     CDS             complement(3294..4586)
                     /locus_tag="PLM04_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HON03077.1"
                     /translation="MKTKKNFHSFPFKLWSLVLLSLPVCLPSQNNFQKNFQKNDSDYV
                     EFSGKIVDVKNGDVLPFATLSVEGSNITTVSNSEGEFSLKIPKEFQDKTLTVSYIGYK
                     NRILKISELKQENSRITLEPMIVSLPEINVISTDAENLVLSVLENRYKNYPEEELLMT
                     AFYRETIKKNRSYVSLSEAIVDILKQPYSSFRQDQAELYKSRKQSDYKKMDTLVFKLM
                     GGPYNSLYMDVMKNPDYLFTESILDNYEFSFDRTTYIDHQLIYIVNFKQRPFVNIPLY
                     YGKLYIDAESLALRSAIFHLDLTDKEKSASMLIRKKPLNAKVFLTNASYRMDYIEKNG
                     KWYYNYSRIELDFKIDWKRKLFNSNYYTVVEMAVTNWEKATEEKKLKNKLRPTVIIAD
                     EASGFSDPEFWGSYNVIEPEKPIESAIKKIQKQLEKRK"
BASE COUNT         1566 a          927 c          875 g         1439 t
ORIGIN      
        1 attaacggga aagtgttttt gcagtcctat tacaaaggat tattggaacg ttagcaatta
       61 ttttcatttc ccaaaattaa ttttacacta acaatttttt ggaaagaatt gatttttcac
      121 aatcgacaaa tagctgaata tcagtcaaaa aaattgaaat aaaattaatg gaataatttt
      181 ctcaggagcg cgtaaacttt ctttttctga taagctgaaa tacaaagccg aaaatcgcaa
      241 gaataacaag cggtagcaac acgttgacaa gttgccattt tagtttttgg tcgtaaacaa
      301 cctgcctgtt gagcaaacga agttttaatg ttttgttttt cagttgcatc catccttcat
      361 catcggcaag gtagagcact gcattttcaa taaaatccct attgccgtat tgcaaacctg
      421 tcaatctgtc gaaacccaaa ggcaagtaga tactgtcacc ctgaactatg gaaatttcat
      481 ttttgataat atctccatct gccacaaaaa tttgttttgt gtttacactt acaggacgaa
      541 tactcgaaat gttcaccacg ctatcgggca acattcggtt ggtaaaaacc gattcaaaat
      601 tgccttccaa aagaacacca actggaacat atccagtatt gaaataattc ttgtcgtttg
      661 cagatggcat atcccgaaga tcaacagtgg ttggagttcc aatcacatga gtattgtcgg
      721 aagttgcaag tagcaaaacg gctgaaactt gttgattctc accaaccaaa tcgatggcag
      781 aacaaaattc ggagcgaacc gtcgcaaggt tacgggtaac gggatgagca taagaagtga
      841 gcaaaagtgg agcaaaaaac cagggaacag gttcgaaatg aggagctttt ccgggttttg
      901 ccacattaac aggcatttgt gcacattgaa catcttgcaa aataaccggt ttgatacgaa
      961 ttccatatcg aaaaagcagg tcggaaagat tcaaatcgag cggaatggca ggtgatgtgc
     1021 cggtaacaga aaaattttct tcagccaggc gcaccccatc aatcaaccaa agcacttttc
     1081 ctccattcat aatgtattga tcgataatca gcttttcttt ttcactgaaa ggaagttgag
     1141 gtttggcaac aattaccact ttatagggat ggagaatggg cgcatcattt cctatgcgac
     1201 ctcgatcgat ttggaaataa cggctcaacg atttgctaat gtcataagtc gcagcttcgg
     1261 acagttcgtc atgtccttcc agaaaagcaa ttttatcaac tgacgtgtta accaaacgac
     1321 ggacagcatc cgtaatttca tactccagtt cgataatgga aatattcaga ttctcgtcgc
     1381 ccgaattgcc gggaatgttt ttcaacagat ttaccaaaac cttgcgtccc ttgtattcca
     1441 tttccaacca cggaaaaatt gttttcctga ttattttgcc gtcgttatcg cgttcatata
     1501 cattggttgg tgtcaatccg cgcgattcca attcggcata atgcttttct ctttcttcag
     1561 gcttgacaac agatgaagga tcttcaaatt caattgctat gggcttggaa ctgtaaactt
     1621 tcagttcttc caacatttcg accgtagatt ttttcaacct taaaaagccg gaattgagac
     1681 tgccatcgag ataaattttt actttcagcg gaaagtcgag ctttttcatc agttttttgg
     1741 atggttccga caaactgtag cgcttttcgg acgtcagatc tactctgtaa tgaacgaagg
     1801 aaagcaccag cgacaacagc aaaaaaactc cccaaaaaaa taattgcttg tgttgaatta
     1861 tattacgcat gttacagaag attcttcagt aaaaatcgag aagaaattcg gatgcaaaat
     1921 taatcatttc ctttgagcaa aaacgcatcc tgaaaaaatt tggtattcag aatcttttct
     1981 ttaccaatac tttcaaaccg gcaggtacaa cccggatatt tatttcctta ccttcttcat
     2041 agggctcgcc atcataatga aaagggccgg gcgcaggtcg aaaaaccgta atttcactca
     2101 ttcggaaagt attgataaac aagtcgttgc ttatagtctt ggtaaaaagt tgcagtgcaa
     2161 gagtaggaag agcaataatg gggaaattac tcataatgga aacatcgagc cgtccatcct
     2221 gaacgctggc ttttggggca atgtacacat tatttcccca ttgagaagca ttagcgacgg
     2281 tcataagaaa agttttggtg gtaaaatcga tatttttccc aataatacgg taattttcag
     2341 aatgataggc aaaataacct ttcacaattt tttccagata agccacaatc cctctgcgat
     2401 ggcctttggc aaattcggca gcaatgtaag cgtcgaaacc tgttccacag gtacagaaga
     2461 aaggtcgttg atttaccaat ccgtaatcga tggaaatttc ttccgaaaaa ttgagcattt
     2521 gaatggcttt tcgcatattc attgggattt ccaaatgtcg cgccaaacca tttcccgaac
     2581 caatgggtaa aattcctaac gaaaccgaag tttgaaccag tccgcgggca acttcgttaa
     2641 tcgttccatc accaccaact gccaccacgc tatcgaaacc cttagcagca aaatctctgg
     2701 ccaataagct tccatgacca cggtagtcgg taaacgccac agttggatca aacaaatctc
     2761 tgtttaattc gtcgtggata tgttgcggga tgaaggactt tgtccttaca ccggccatag
     2821 gattgataat gaaagcaatt ttctttttca tggcagaaaa acgtgcgttc aaaaaacggc
     2881 tgcgaagtta caaaaaacta ttaaactgca aaattcatca ataattattc aacattatag
     2941 aatccggtta agttcaactt agaagtgcga attaagcaaa aagttttaca ttgtttttta
     3001 ataaacccaa agcaccacag ctgataggca aacgactgga aggaagtaaa agcttgaaag
     3061 agcattaaaa tgaaccgaca acataattca ccaaaaaagg agaccgaagt cttcctaaaa
     3121 ttaatactgt ttatgaaacc cacatgtatt gcatacacaa acacgaatga atataatatt
     3181 tagacatgtg ggttccatca gacctttaaa aaataaatta tattctgatg aaagacaaaa
     3241 atttgagcaa tcggtagtcg caaagtttct cggcagagaa aatgacgaaa aaattacttt
     3301 cttttttcga gttgtttctg aatctttttt attgctgatt ctatgggttt ttcgggttca
     3361 atcacattgt aactgcccca aaattcagga tcagaaaagc cggaagcttc atctgcaata
     3421 attaccgtcg ggcgcaactt gtttttcaat ttcttttctt cagtagcttt ttcccaattg
     3481 gtaacggcca tttccactac cgtatagtaa ttggaattga ataattttct cttccagtca
     3541 attttaaaat ccaattctat ccgactgtag ttatagtacc atttcccatt tttttcaatg
     3601 taatccattc gatacgaggc atttgttaaa aacacttttg cattaagggg ttttttgcgt
     3661 atcagcatgg aagctgattt ttctttgtcg gtaagatcaa gatgaaaaat ggcacttctc
     3721 aaggcaagac tttcagcatc aatgtaaagt tttccataat ataacggaat gttgacgaat
     3781 ggccgttgct taaagttgac aatataaata agctgatgat cgatgtaagt agtccgatca
     3841 aaagaaaatt cataattatc caagatactt tcagtaaaaa gatagtcggg atttttcatc
     3901 acatccatgt aaagcgaatt gtaaggacca cccatcaact taaaaaccaa tgtatccatt
     3961 tttttatagt cggattgttt cctgctttta tacaattctg cctgatcctg ccggaatgaa
     4021 gaataaggct gtttaagaat atccacaatg gcttcagaaa gcgatacata agagcgattt
     4081 tttttgatgg tttctctgta aaaagctgtc attaagagtt cttcttccgg ataatttttg
     4141 tagcgatttt ccaatactga cagtaccaaa ttttctgcat ctgttgaaat cacatttatc
     4201 tcgggcaaag aaacaatcat gggttccaaa gttatgcgag aattttcctg ttttagctcg
     4261 gaaattttaa gaattctgtt tttgtatcct atataactga ccgttagtgt tttatcttga
     4321 aattcttttg gaattttcaa ggaaaactct ccttcggaat tggaaaccgt agtgatattg
     4381 cttccttcta ccgaaagagt ggcaaatggc aatacatctc cgtttttaac atctacaatt
     4441 ttacctgaaa attccacata atcagaatcg ttcttctgaa aatttttttg aaaattattt
     4501 tgcgatggca agcaaacagg gagcgataac aaaactaagc tccataattt aaacgggaaa
     4561 gaatggaaat ttttttttgt tttcatggcg gcaagttata aagagtagaa ccggataaat
     4621 tgttgaatct acgtgaaaaa ttcttacaaa caaagataac acattcaaca tggaattgtg
     4681 ctatatttca cttaaaaata acaatttggc tggacaaaag tttgagacat caaatgccgc
     4741 aaaaacaaat aagaaaccca catgtattgc atacacaaac acgaataaat ataatattca
     4801 gacatgt
//
LOCUS       DAOTAX010000104         4751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14870_length_4751_cov_31.055153, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000104 DAOTAX010000000
VERSION     DAOTAX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14870_length_4751_cov_31.055153"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLM04_08150"
     CDS             complement(<1..1253)
                     /locus_tag="PLM04_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HON03078.1"
                     /translation="MKEELLIYKLSETIKKTSQIDKELFTKYNVKRGLRNEDHTGVLV
                     GLTRIGDVVGYERTEDGQLKAIPGKLYYRGIDVEELVHGIQSENRLGFEETAFLLLSG
                     YLPTKDELETFKKILNEAMPLQHQATMNIMALKGHDIMNILARCVLEQYIYDEDPDNI
                     SRDNLIRQSINLVSKFPTIIAYAYNVLRHHEKGRSLHVRHPDENFSFAENFLFMIKGK
                     GQYTPLEIKILDLALILHAEHGGGNNSTFSVRVTSSTETDTYSSIAAGIGSLKGPLHG
                     GANLKVMGMIKEMRENINDWTDADEIDQYLKKLLNKEAYDKTGLIYGIGHAVYTISDP
                     RAILLKDMARELAKEKGLEKEFAFQELIEQRAVEVFMNYKNGNNNKQVCVNVDFYSGF
                     VYEAIGIPKEVYTPIFAMARIVGWCA"
     gene            complement(1344..3593)
                     /locus_tag="PLM04_08155"
     CDS             complement(1344..3593)
                     /locus_tag="PLM04_08155"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HON03079.1"
                     /translation="MIFDEAMIQQFYESFPEKIEQIKNRLGRPLTLTEKILYTHLSAD
                     ETLKNFVRGIDYVNFSPDRVAMQDATAQMALLQLMNSGRSKTAVPATVHCDHLIQAHV
                     SAENDLDTAIKTNSEVYNFLRAVSNNFGIGFWEPGSGIIHQVILENYAFPGGLMIGTD
                     SHTPNAGGLTMLAVGVGGADAVDVMSGLPWELKMPKIIGVKLTGKLSGWTSPKDVILK
                     LTGMLTVKGATNAVIEYFGEGTASLSATGKATICNMGAEVGATSSIFPFDNKSAVYLE
                     ATGRNSIAETAKKLAEHLQADPEVVQHPEKYYDRLIEIDLSTLEPHINGPFTPDAAYP
                     ISIFGKVVREKNYPQKLEVGLIGSCTNSSYEDLSRAVSVLRQAREKNLKVRSQMIINP
                     GSEKIRSTAERDGMLDEFKSSGATIMANACGPCIGQWKRDIDVADRPNSIITSFNRNF
                     AKRNDGNPNTHTFLSSPEIVAALSIAGDLCFNPLTDELENEKGEKIKLDEPQGFELPL
                     LGYEIKESGYIAPSEEKMEINIDPKSERLQLLQPFPAWDGKDMIELPLLIKVKGKCTT
                     DHISMAGPWLRFRGHLDNISNNLLMGAVNACNDKTNAVLDPETKQYTTVSALARKYKA
                     KGTGSVVVAEENYGEGSSREHAAMEPRYLNVKAIIAKSFARIHETNLKKQGMLALTFI
                     DKSDYDKIREDDRISILGLSDFKPGKNLLIQLHHSDGSVEQFEVAHSFNKTQIEWFKA
                     GSALNAMRK"
     gene            4433..>4751
                     /locus_tag="PLM04_08160"
     CDS             4433..>4751
                     /locus_tag="PLM04_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03080.1"
                     /translation="MKKFFYSFGSCLPAFSLAMFLFNFSLILQVSAQGAGKALNFNGT
                     HKYVSVANSSTLNVSNAMTLEAWMKSAEENFSYRVPITLNPATPIADYQVKVELTTSN
                     FAYA"
BASE COUNT         1370 a          929 c          875 g         1577 t
ORIGIN      
        1 gcacaccagc caacaatgcg ggccattgca aaaataggcg tgtaaacttc tttcggtatt
       61 ccaatggctt cgtaaacaaa tccagaatag aagtcgacat ttacacaaac ctgcttgttg
      121 ttgttcccat ttttataatt cataaatacc tctacggcac gctgttctat cagttcctga
      181 aatgcaaatt ctttttcaag gcctttttct ttggcaagtt cgcgagccat atctttcaac
      241 aatatggcac gcggatcgct gatggtataa acggcgtgtc caattccgta aatcagtccg
      301 gttttatcgt aagcttcctt gttcaataac tttttcagat attgatcaat ttcatcagcg
      361 tcagtccaat cattaatgtt ttctctcatt tctttgatca ttcccattac tttcaggttg
      421 gcacctccgt gaagcggccc tttcaacgaa ccaatgccgg cagcaatgga agaataggtg
      481 tcggtttcgg tagagctggt tacacgtacg ctaaaagtgg aattgtttcc tccgccatgt
      541 tcggcatgaa gaatcaaagc caggtcgaga attttaattt ccaggggtgt atattgtcct
      601 tttcctttta tcatgaacaa gaaattttca gcaaaagaaa aattttcatc cgggtggcgg
      661 acatgaagcg atcgtccttt ttcgtgatgg cgaagaacgt tataagcata ggcaatgata
      721 gttggaaatt tggaaaccag attgatggat tgccgaataa gattgtcgcg tgatatgttg
      781 tcgggatctt catcgtaaat atattgttcc aaaacgcagc gcgccaaaat attcatgatg
      841 tcgtgtcctt tcagtgccat aatgttcatg gttgcctgat gttgaagagg catagcttca
      901 ttgagtattt tcttgaaagt ttccagttcg tctttggtag gcagataccc tgataacagc
      961 aaaaaagcag tttcttcaaa accaagacga ttttcggact gaataccatg aaccaattct
     1021 tctacatcaa ttccgcgata gtaaagttta cccggaattg cctttaattg gccatcttcg
     1081 gtacgttcgt agcctactac gtccccaatg cgggtcaaac ctaccagtac tcccgtatga
     1141 tcttcgtttc ttaaaccacg ttttacgttg tatttggtaa acaattcttt gtctatttgg
     1201 ctggtttttt tgatagtctc agataatttg taaattaaaa gttcttcttt cattttttac
     1261 ctctattttt tattgataag tttctcttat attaatttgt gcttttggaa aataattttt
     1321 cttgagtttt ggaacaaaaa gtttcatttt ctcatggcat tcagtgcact accggcttta
     1381 aaccattcaa tctgagtttt attgaaactg tgtgccactt caaattgttc gacacttcca
     1441 tcggaatgat gaagttgaat caacaaattt tttcccggtt tgaagtctga aagtccaaga
     1501 atgctaatgc gatcatcttc gcgaatttta tcgtaatcgg acttgtcgat aaaggttaaa
     1561 gccagcatac cctgcttttt caggtttgtt tcatgtattc gggcaaatga tttggcgatg
     1621 atggctttaa cattcagata gcggggttcc attgctgcat gttcacgaga cgaaccttcg
     1681 ccgtagtttt cttcagcaac taccacagag cctgtacctt ttgccttgta tttgcgtgcg
     1741 agagccgaaa ctgtggtata ttgtttagtc tccggatcga gtacggcatt ggttttatca
     1801 ttacaagcat tgacggcacc catgagcaaa ttgttggaaa tattgtccaa atgtccccga
     1861 aaacgaagcc acggacctgc cattgaaata tgatcggtag tacatttgcc tttaactttt
     1921 atcagcaatg gcaattcaat catatctttg ccatcccatg ccggaaaagg ttgcaatagt
     1981 tgtaatcttt ctgatttggg atcgatattg atttccattt tttcttccga aggcgctata
     2041 taacccgatt cttttatttc gtatcccaac agaggaagtt caaaaccttg tggttcatcc
     2101 agtttgattt tttctccttt ttcgttttcc agctcgtcag tcaacggatt gaaacacaaa
     2161 tcgccggcaa tagaaagagc tgccactatt tcgggagaag aaagaaaagt atgggtattg
     2221 ggattgccat cgtttcgttt ggcaaaattt ctgttaaaag aagtgataat ggaatttgga
     2281 cgatccgcta catctatgtc gcgtttccat tgcccaatgc aagggccgca agcatttgcc
     2341 ataattgtgg ctcctgaact tttaaattca tccaacatgc catcgcgttc ggctgtactt
     2401 cttatttttt cggatccggg attaataatc atttgcgacc gaactttcag atttttttcc
     2461 cgtgcctgac gcaataccga aacagctcgt gaaagatctt cataggaaga attggtacat
     2521 gaaccgataa gcccgacctc caatttttga ggataattct tttctctcac cactttccca
     2581 aaaattgaaa tcggataagc tgcatccgga gtaaagggcc cgttgatgtg aggttccaat
     2641 gtgcttaaat caatttcaat cagtcggtcg taatattttt cgggatgttg tacaacttcc
     2701 ggatcggcct gtaaatgttc ggccaatttc tttgcagttt cagcaattga attacgtcct
     2761 gtagcttcga gatagacagc cgatttgtta tcgaaaggaa aaatagagct ggttgctcca
     2821 acttctgcgc ccatattgca aatagtagct tttcccgtag ccgaaagtga agccgttcct
     2881 tctccaaaat attcgattac ggcattggtt gcacctttta cggttaacat gccagtaagt
     2941 ttaagaataa catctttcgg agatgtccag ccggaaagtt ttcccgttaa tttcacaccg
     3001 attattttag gcattttcaa ttcccacggc agtcctgaca tgacgtcaac agcatcagca
     3061 ccaccgacgc ccacagccaa catggttaat cctccggcat tcggtgtatg cgaatcggta
     3121 cctatcatca acccgccggg aaatgcataa ttttccaaaa ttacttgatg gataattccc
     3181 gaaccgggtt cccaaaaacc gataccgaaa ttgttggaaa cagcccgtaa aaaattgtac
     3241 acttcgctat tcgtttttat tgcggtatcc aaatcatttt cagccgaaac gtgagcttgt
     3301 atcaaatgat cgcaatgaac tgtagccgga actgccgttt ttgaacgtcc cgaattcatg
     3361 agttgcaaca gagccatttg agccgtagcg tcctgcatgg ctactctgtc gggtgagaaa
     3421 ttgacataat cgatgccacg aacaaaattt tttaatgttt cgtcggccga gaggtgagta
     3481 tagagtattt tttctgttaa agtcaatggt ctgccaagcc ggttttttat ctgttcaatt
     3541 ttttcaggaa aggactcgta gaattgttgg atcattgctt catcaaaaat catattttct
     3601 gtatttttac tcatatttga atattttgtt ttgcaaaagt aataaaaaaa gaacaaaata
     3661 atactttttg tccaataata attaaatttt taaataatca tttgcatttc ccgaacgaaa
     3721 tgtatattta tacaaacaaa tcgacatgtc ataatgtttt ccaataacat attttattaa
     3781 atgataagtc ataaagtttt ctatatcaaa cgggataaat aattgacaac attcataatt
     3841 ttagaaatga gttattaaaa aatctatcat tagtcgattt ttacgatatg tactgcaata
     3901 aattcacatt tatccctaca ctcaaaatct gcaacaagga aaagatttta gttttggagg
     3961 aaatggcaat gtttaaaaat acaaaacagt tgtttgtgaa tctcgatata tccattacaa
     4021 acaaagagat aacaccatat aacggagtat cattttgaaa caaatgttag aatctgctta
     4081 aatacaattg agacaagctc taactatgga gcactgcgac tttgactttt cctttgaatt
     4141 ggctaatatt tctggtttaa actatcacat tcgaagcatt gataaaaaac aattattttg
     4201 ttttatttta ttgtaaaaat ttgactaaat cggtacaatt tctattattt attcatatat
     4261 ttgttattta atacgtaata gagttaatat ttgattattg aaatggtttt aagcgaaatg
     4321 aagaaaattg cctctattaa aagagttgga tttgtatctt actattttgt ttgttatcaa
     4381 aatacgaatc agaaaacttt acacattacc aatcttaaac aattgataaa ttatgaaaaa
     4441 atttttttac tcatttggct cttgtttgcc tgcattttcg ctagccatgt ttttgtttaa
     4501 tttttccctt attttgcagg tttcagctca gggagcaggc aaagctttga attttaacgg
     4561 gacacacaag tatgtttctg tggccaactc ttcaacatta aatgtttcga atgccatgac
     4621 actggaagca tggatgaaat cagcggaaga gaatttcagt tatcgagtac cgattacttt
     4681 gaatccggca acacctatag ccgattatca ggtaaaagtt gaattaacaa cttccaattt
     4741 cgcttatgct c
//
LOCUS       DAOTAX010000105         4733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_14937_length_4733_cov_34.806327, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000105 DAOTAX010000000
VERSION     DAOTAX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14937_length_4733_cov_34.806327"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /gene="tig"
                     /locus_tag="PLM04_08165"
     CDS             <1..1386
                     /gene="tig"
                     /locus_tag="PLM04_08165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HON03081.1"
                     /translation="KNYKYQKTNSMNIVKKDLDQNNAVVTLHVEKADYAENVEKNLRD
                     LRKKANIPGFRPGMVPMSLLKKMYGKSVMADEINKLIAEKLYDYIRENDLNILGDPLP
                     NETEQPEIDFNTQEEFDFVFDLGLAPDFDIELSKEIKINYYNIAVTDQMIEDRIKSYS
                     QRFGRYEEVETVEENDMLRGEVIELENGEEKQNGLQVSEAILTPAYIADPEQKALFLN
                     AKKGDTVIFNPAKAFGNETELASFLKISKEQAKEITSDFSFKINDITRYFDSEINQEL
                     FDKVFGENAVTNEQEFREKVKETIATNLKENSDYRFGIDAKKTLVEKFKNLSFPDAFL
                     KRWLLASNKNLTNEQLESDYPKMIEDLTWQLIKEKLIKKFDIKVNEVEVENYAKEFAR
                     AQLAQYGISGADEELISNYAKSLLEKEDTLRNFIERAGDNKVIVTIKDSVEVEVKEIS
                     PEEFDKLFEEN"
     gene            1504..2202
                     /gene="clpP"
                     /locus_tag="PLM04_08170"
     CDS             1504..2202
                     /gene="clpP"
                     /locus_tag="PLM04_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HON03082.1"
                     /translation="MNKKNIRSDFRNYATRHLGMNGLAIDQYAGFINDSYISPTILEE
                     RQLNVTQMDVFSRLMMDRIIFLGTEIDDYASNVIEAQLLYLDSAEPGKDISLYLNTPG
                     GSVYAGLGIYDTMQYISSDVSTICTGMAASMGAILLVAGTKGKRFALKHSRIMIHQPM
                     GGAQGQASDIEITAREIQKMKQELYNIIAEHSGNPYEKIEKDADRDYWMTSQEALEYG
                     MIDKVLINERKSKK"
     gene            2213..3442
                     /gene="clpX"
                     /locus_tag="PLM04_08175"
     CDS             2213..3442
                     /gene="clpX"
                     /locus_tag="PLM04_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HON03083.1"
                     /translation="MPKNVRHCNFCGRSEAEVEFMILGQDGVQICNECAIQAAEIVRE
                     SARKSKSSHLHIEKIPKPKEIKQYLDQYVIGQEEAKKYLSVAVYNHYKRLMQKTEEDE
                     VEIEKSNIIMVGPTGTGKTLLARTIAKKLHVPFTIVDATVFTEAGYVGEDVESILTRL
                     LQVADYDVKAAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSVVNVP
                     PQGGRKHPEQKMIPVNTQNILFICGGAFDGIEKKIAARLNTRVVGYGASEENKLIDRN
                     NLLQYIAPQDLKSFGLIPEIIGRLPILTYLEPLDRDALRRILTEPKNSIIKQYVKLFK
                     MDNIELIFEDEVLDYIVDKAIEFKLGARGLRSITETIMMDSMYEMPSKKSTKWVVTLP
                     YAQSKIEKTNVHRLKIA"
     gene            3715..3927
                     /locus_tag="PLM04_08180"
     CDS             3715..3927
                     /locus_tag="PLM04_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03084.1"
                     /translation="MIFAKEKPEDAKKIIGEKRWNEIHKETGLEGGLGHKFYEEYRST
                     SKISKEGRKRRNELIDLSTKYYNKFR"
     gene            3924..4154
                     /locus_tag="PLM04_08185"
     CDS             3924..4154
                     /locus_tag="PLM04_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03085.1"
                     /translation="MNNTDYIYENFQKGNEIYIMDDIEEVAIRYSYSKDGYKTFAKFK
                     GGREYKIDETSNIVTRADMGGTILTKEQYKKF"
BASE COUNT         1767 a          811 c          928 g         1227 t
ORIGIN      
        1 aaaaattata aatatcagaa aactaacagt atgaacattg ttaaaaaaga cctcgatcaa
       61 aacaatgcag tagtaactct gcacgttgaa aaagccgatt acgccgaaaa tgttgaaaaa
      121 aatcttcgcg atttacgcaa aaaagctaat attccaggct ttcgtcccgg aatggttccg
      181 atgagccttt tgaaaaaaat gtatggcaaa tctgtcatgg ctgacgaaat aaacaagctg
      241 attgcagaaa aactttatga ttatattcgc gaaaacgatt tgaatatatt gggcgatcca
      301 ctgcccaacg aaactgagca gcccgaaata gatttcaata ctcaggaaga gtttgatttt
      361 gttttcgatc tggggctggc tcccgatttt gatatagaat tgtcgaaaga gatcaaaata
      421 aattattaca acattgctgt aaccgatcaa atgatagaag accgcataaa aagctatagt
      481 caaagatttg gtcgctacga agaggtggaa acggtagaag aaaacgatat gcttcggggc
      541 gaagtcattg aattggaaaa cggtgaagaa aagcaaaacg gcttgcaagt ttcggaagct
      601 atcctcacac cagcctacat cgccgatcct gaacaaaaag cgttgttttt aaatgcaaaa
      661 aaaggcgata ccgttatctt caacccagcc aaagcattcg gcaatgaaac agaactggct
      721 tcattcctga aaatttcaaa ggaacaggct aaagaaatta cttccgattt cagtttcaaa
      781 attaacgata ttacccgcta cttcgatagc gaaatcaatc aggaattgtt cgataaagtt
      841 ttcggagaaa atgcagtaac caacgaacag gaatttcgcg aaaaagtcaa ggaaactatc
      901 gccaccaatt taaaagaaaa cagcgactat cgatttggca ttgatgccaa gaaaacattg
      961 gtggaaaaat tcaagaatct ttctttccct gatgctttcc tgaaacgctg gttgttggct
     1021 tccaacaaaa atttgaccaa cgaacaattg gaatcggatt accctaaaat gattgaagac
     1081 ctcacttggc aacttatcaa ggaaaagctg ataaaaaaat tcgacatcaa agtgaatgaa
     1141 gtagaagttg aaaattacgc caaagaattt gccagagcac agcttgctca gtacggtata
     1201 agcggagccg acgaagaatt aatttccaac tatgccaaga gcttgcttga aaaggaagat
     1261 accctgagaa atttcatcga gcgcgcaggc gacaataaag ttattgtaac cataaaagat
     1321 agtgttgaag tagaggtaaa agaaatcagt cctgaagaat ttgacaaatt atttgaggaa
     1381 aattaaaaaa attttcattg aaaacacttc attgaaaaca aaaacctttt taatttgttt
     1441 atatcagaac tttttcaggt agatattaaa aaaaaacctt aaattaaaaa gaaaggaaac
     1501 aatatgaaca aaaaaaatat aagaagcgat tttcgcaatt atgcaacccg tcatctggga
     1561 atgaacggtt tagctatcga tcagtatgcc ggttttatta atgacagcta tatttctccc
     1621 actattctgg aagaacgtca gctgaatgtt acacaaatgg acgttttctc ccgattaatg
     1681 atggacagaa ttatttttct gggcaccgaa atcgatgatt acgcttcaaa cgttattgaa
     1741 gcacagttgc tttaccttga ttcggccgaa ccggggaaag atatttctct ttacctgaat
     1801 actccgggcg gatccgttta tgccggattg ggaatttacg acaccatgca gtatatcagt
     1861 tccgatgttt caaccatttg cacgggtatg gcagcgtcta tgggcgccat tctgctggta
     1921 gcaggaacca aaggcaaacg gtttgccctg aaacactcgc gtatcatgat tcatcaacct
     1981 atgggcggag ctcagggaca agcttccgat atagaaatta cggcacgcga aattcaaaaa
     2041 atgaaacagg aactttacaa cattattgcc gaacattcag gaaatcctta cgaaaaaatt
     2101 gaaaaagatg ccgaccgcga ttattggatg acttcacagg aagcacttga atacggaatg
     2161 atagataaag tgttgattaa cgagcgaaag agtaaaaaat aatattacaa gtatgccaaa
     2221 aaacgttaga cattgcaatt tttgcggacg ctctgaagct gaagtagaat ttatgatttt
     2281 aggtcaggat ggcgtgcaaa tttgtaatga atgcgccatt caagcagctg agattgtcag
     2341 ggaaagtgcc cgaaagtcca aatcttccca tttgcatatc gaaaaaatac ccaaaccaaa
     2401 agagattaag cagtatctcg accaatatgt tatcggtcag gaagaagcaa aaaaatatct
     2461 ttcggtggct gtctacaatc attataaacg tttgatgcag aaaacggaag aagatgaagt
     2521 tgaaatagaa aaatcaaaca ttatcatggt tggccccaca ggaacgggta aaacgttgct
     2581 ggcacgaacc attgccaaaa agttgcatgt acctttcacc atcgttgatg caacggtttt
     2641 caccgaagca ggttatgtgg gcgaagatgt agaaagtatt ttgacccgat tgttgcaggt
     2701 tgccgattat gatgtaaaag cggccgaaag aggaattgtt tttatcgacg aaatcgacaa
     2761 aatcgcccgc aaaagtgata atccgagtat tactcgcgac gtaagcggag aaggcgttca
     2821 gcaaggtttg ctgaaattgt tggaaggatc ggtggtgaat gttccgcctc aaggtgggcg
     2881 aaaacatccg gaacagaaaa tgattcccgt caacacgcaa aacattttgt ttatttgtgg
     2941 tggcgctttt gatggaatag aaaagaaaat tgctgccaga ttgaatacaa gagtggtcgg
     3001 atacggagct tcggaagaaa acaaactcat cgacagaaac aatcttcttc aatatattgc
     3061 tccgcaagac ttgaaatctt tcggccttat tccggaaatt atcggacgtt tgcccattct
     3121 aacttatctg gagccgctcg atcgcgatgc gttgcgtcgc attcttaccg aaccaaaaaa
     3181 ttcgattatc aaacaatatg tcaaactatt caaaatggat aacatagaat tgatttttga
     3241 agatgaagtg ttggactaca ttgtggataa agctatagag ttcaaattgg gcgccagagg
     3301 tctgcgttcc attaccgaaa ccatcatgat ggattcgatg tacgaaatgc catccaaaaa
     3361 atctaccaaa tgggtggtta cgctccctta cgctcaaagt aaaatcgaaa aaacaaatgt
     3421 tcaccgatta aaaattgcgt aatgtaactt tctgaaaaaa tagataaata gaaaaataca
     3481 gaatatttta ttggcaacat tcaagtagaa aatgcaactt tttgattaaa tataaaaata
     3541 ggcgattcgt aattaaatct ttttcttggt tgattattga taatataaaa aaatactatc
     3601 tttatacgtg taaaaaatca aacaccagaa aaatttcaag gctattcagg agtgttagca
     3661 aatgtaagaa caaaagatgg aatcgtcgga gagattcaat ttaataccga ccgaatgata
     3721 tttgcgaaag aaaaaccaga agatgcaaaa aaaattattg gtgagaaaag gtggaatgaa
     3781 atacacaaag aaacaggtct tgaaggtgga ttggggcata aattttatga ggaatacaga
     3841 agtacatcta aaatatctaa agaaggcaga aaaagaagaa atgagctaat agatttgtca
     3901 acaaaatatt ataacaaatt tagatgaaca acacagatta catttacgaa aacttccaaa
     3961 aagggaatga gatttacatc atggacgata ttgaagaagt cgcaatacga tattcctatt
     4021 caaaagacgg ttacaagacg tttgcaaaat ttaaaggagg aagggaatat aagattgatg
     4081 aaacttcaaa tattgtaacc agagccgaca tgggaggtac gatattaact aaggagcaat
     4141 ataagaaatt ctgattagcg gacaaggcta tttgcgcatg aaacaaggcg gtgggatgcg
     4201 agtggctaat cggatgttta ggcgggtaac accgcctttt tttattgcaa caaaattcca
     4261 tcatttttta tgcaaataca tgaaagacaa aacgttaatt aatcataaaa caaagtttca
     4321 ggtcgaaaat ttaacactaa aaatttctat ataacaaata tatctattat ccttatgata
     4381 tcaaaataac cactaaaaat aacagaaaat ggaaacaata aaattagaaa gcccagtaat
     4441 catgcacaag aaatttcaaa aagagctgtt attaaagcag gcgaacagtt tgttaacatg
     4501 tacggcatcc ttattgaaaa agcaattaat acgaaaaaaa ttgctataaa aaagaatggt
     4561 agccacgcct gaccaccaat cttccttatt aaccttaaat ctaataccat gaaaaaaatc
     4621 acactgcaaa gataaatgtt tttaattatt ttttccaggt ctttctcaaa aatttttcat
     4681 aaaaaaggaa aatataacaa gtttttaatc aaatgaaacc cacatgtatt gca
//
LOCUS       DAOTAX010000106         4702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_15044_length_4702_cov_30.716376, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000106 DAOTAX010000000
VERSION     DAOTAX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15044_length_4702_cov_30.716376"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLM04_08190"
     CDS             complement(<1..737)
                     /locus_tag="PLM04_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HON03086.1"
                     /translation="MIRKFFVLFFGFNIFIDSLLWAIDPVTALPIPKQVVFANETVSL
                     ERIDMRERYDREQTVIAYNHSTSILLLKRANRFFPVIEPILKNNGIPDDFKYLAVVES
                     NLDLRAFSPAQAAGIWQLMPKTAEQYGLEVNDQIDERYYLEKATVAACQFLKSAYAKY
                     HSWVTVAASYNAGMAKISGEQEKQQVEDAYDMLLSTESSRYIFRILAMKQFFENPKLF
                     GYQLKKDDFYPYIPTKEIVVSTSVEDWT"
     gene            1096..1548
                     /gene="coaD"
                     /locus_tag="PLM04_08195"
     CDS             1096..1548
                     /gene="coaD"
                     /locus_tag="PLM04_08195"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HON03087.1"
                     /translation="MKRAIFPGTFDPFTIGHYSIVKRGLKLFDEIIIGIGVNQAKKTL
                     FSIEKRQDIISQTFKSEPRVKVSIYDSLTVDFARSVDAGFILRGLRSVSDFEYEHNIA
                     DANRKISGIETVILFTDYEYSYISSTVVRDLIVYGKDISIFLPPNVKI"
     gene            1758..3410
                     /locus_tag="PLM04_08200"
     CDS             1758..3410
                     /locus_tag="PLM04_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON03088.1"
                     /translation="MKKKKFFLFLLIFCSPFFLVAQQDNQRTFRISKNLNIYNSLLRE
                     LDMFYVDTLNYDKLIKTSIDEMLNTLDPYTIYLPEEETEDLTFMTTGEYAGIGALIMK
                     NGNEIVVSEVYEGMPAQRNGIRPGDIIVEVDGKKTDGFAVNDVSNLLRGTPNSQINIK
                     IKRYGEKKIISKTFLREKIQINPIGYAAEVDDKTGYLLLNEFTENAALGVKSAVNDLI
                     KNHHIESLVLDLRNNGGGLVDEAVQILGYFLPKGTTVVTTKGKNNILDRIYKTPTEPV
                     FPNLKLAILVNRSSASASEILAGAIQDLDRGVIVGERTFGKGLVQNIRNISYGGHLKV
                     TTAKYYIPSGRCIQAIDYSHRNEDGSVGYVPDSLTSEFLTKNGRKVRDGGGIIPDKQT
                     ESKGKLNVAHYIFAQNLYFDFATRYASLHPTIASPKDFKLSEDDFNNFVDYLHEKNFT
                     YSYQTNKYFQQLYQVAKIEGLDSIAQSEFEALKTKLTPNVDADIRENKDEIEALLSLE
                     IIKRYYYQKGQIEFSLRTDDDLKEAIQILKSEETYRKILSAI"
     gene            3527..4123
                     /locus_tag="PLM04_08205"
     CDS             3527..4123
                     /locus_tag="PLM04_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HON03089.1"
                     /translation="MAVITPFIMEKKNIGEKILALCKSRNLSIQDLVDTTGLSEVQIN
                     RVIESETIPSLAPLIKIARALGVRLGTFLDDAEELGPVVHRSGEVQQPASFSSQLSDA
                     NSHLDFYALASRKTNRHMEPFFIDIQPSSTHEPIFSSHEGEEFIFVLEGAAKIDYGNQ
                     TFFLHPGDSIYYDSIINHLVSSADEKPAKILAVVYTPL"
     gene            4227..>4702
                     /locus_tag="PLM04_08210"
     CDS             4227..>4702
                     /locus_tag="PLM04_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HON03090.1"
                     /translation="MELFEKTIGEWLEYWANETPEREYLVYADRNLRFTWKSFNERVD
                     NMAKGLMAIDVKKGDHVGIFAQNVPDWLTFLYACAKIGAIAVTLNTSCKEHELAYIIE
                     NSDMHTLCMTEGDNGNDFIKTIYSLIPELKTSERGRLKSNRFPVLKNVVFIGQEKH"
BASE COUNT         1578 a          935 c          853 g         1336 t
ORIGIN      
        1 gtccaatctt caacggaagt acttacaaca atttcttttg tgggaatata cggataaaaa
       61 tcatcctttt tcaattgata accaaaaagt tttggatttt caaaaaactg tttcatggcc
      121 agaattctga aaatatatcg cgaactttcg gttgaaagca acatatcgta ggcatcttct
      181 acctgctgtt tctcctgttc acccgaaatt tttgccatac ctgcattata ggaagctgcc
      241 accgtaaccc aactgtgata ttttgcatag gcactcttca aaaattggca ggccgcaaca
      301 gtagcctttt ccaagtaata acgctcatca atttgatcgt tgacttccaa cccgtactgt
      361 tcggcggtct tcggcattaa ttgccaaata ccggcagcct gagccggaga aaaagcacgc
      421 agatcgagat tgctctctac aacagccaaa tatttaaaat catccggaat accgttgttt
      481 tttaaaatag gttcgattac ggggaaaaac cgattggcac gcttcagcaa taaaatgctg
      541 gtggaatggt tataagctat caccgtctgt tctctatcgt aacgttcacg catatcgatt
      601 ctttccaaag aaacagtttc attggcaaaa acaacctgtt tgggtattgg cagtgccgta
      661 accggatcaa tagcccacaa aagggaatca ataaaaatat taaaaccaaa aaacaaaaca
      721 aaaaattttc gtatcatcac aaaatctttc aattaattgc agcaaagtta ataaaccatt
      781 ttaaaactga aaataaaatg caaaaaaatg cacttcgatg cagaatttag ttggccataa
      841 aagaattgaa aaaataaaac ctgaaaccaa tgaacgacgt ctgaaaattc tgtaagggga
      901 actcgagaaa aaccaatttt atcagcgata tacagaaatt gaaaattatc atgtaaattt
      961 gcacataaat aaatttaatc aaaaatgaat ggacatccct tgttgcaaaa aatggtttcc
     1021 ttagtagcaa aaaagtgcga aatgagggca actttccttc aaccaagttc ttgtcgattc
     1081 aaaataattt agatcatgaa acgagccatt ttcccgggaa cgtttgaccc atttaccatt
     1141 ggacactaca gcattgtcaa gcgaggattg aagcttttcg atgagataat tattggcatt
     1201 ggagtcaatc aggcaaaaaa aaccttattc tccatcgaaa agaggcagga cataattagt
     1261 caaacattta aaagtgagcc aagagtgaag gtttctattt acgacagcct cacagtggat
     1321 tttgcccgtt cagtcgatgc aggtttcata cttcggggat tgcgctctgt tagcgatttt
     1381 gaatatgaac acaatattgc cgacgccaac cgaaaaattt ccggaattga gacagtcatt
     1441 ctgtttaccg attacgaata ttcgtatatt tcttcaactg tggtgcgaga tttaattgtt
     1501 tacggaaaag acatttcgat ttttctgccg cctaatgtaa aaatttaata aattcatttt
     1561 cgatcattta tattcattca aacgaaaaga aataaaaata atttccctaa gaattttgtt
     1621 ttcctacaaa atccattcaa gaaaaaaatt aatgacaaac gcatgaaacc cacatgtatt
     1681 gcatacacaa acacgaataa atataatatt cagacatgtg ggttccatca gacctttaaa
     1741 aaataattta tattctgatg aaaaagaaaa aattcttcct gttcctccta attttttgct
     1801 ctccattttt tcttgtggct cagcaagata atcaacgtac atttcgtatc agcaaaaatc
     1861 tgaatattta caattcattg ctgcgtgagt tggacatgtt ttatgtcgat accctgaatt
     1921 acgacaaact gataaaaact tctatcgacg aaatgttgaa cacactggat ccttatacaa
     1981 tttatctgcc cgaagaagaa accgaagacc ttacctttat gacgacaggc gaatatgccg
     2041 gtattggtgc acttatcatg aaaaacggaa atgaaattgt tgtttccgaa gtttatgaag
     2101 gcatgccggc gcaacgtaac ggtattcgac ccggggacat cattgtggaa gtagatggga
     2161 aaaaaaccga tggctttgcc gtcaacgacg ttagcaattt attgagaggt actcccaata
     2221 gccaaatcaa cataaaaatc aaacggtacg gagagaaaaa aatcatcagc aaaacttttc
     2281 tgcgtgaaaa gattcaaatc aatcccattg gctatgcggc agaagttgat gacaaaacag
     2341 gctatctgct gctcaacgaa ttcaccgaaa acgcagcgtt gggagtaaaa tcggctgtaa
     2401 acgatttgat aaaaaatcat catattgaat ctcttgtact cgatttacgc aacaacggag
     2461 gcggattggt ggacgaagcc gttcaaattt tgggatattt tttgcccaaa ggcacaacag
     2521 tggttaccac caaaggaaaa aacaatatac tcgacagaat ttataaaact cccaccgaac
     2581 ctgtttttcc caacctcaag ctggcaattt tggtcaaccg atcttcagcc tctgcctccg
     2641 aaatactggc cggagccatt caggatctcg atcgcggtgt aatagtcgga gaacgtacat
     2701 ttggcaaagg attggttcaa aacattcgca acatcagtta cggcggacat ttgaaagtta
     2761 cgacagcaaa atattacatt ccgagtggca gatgcataca agccatcgac tacagtcatc
     2821 gcaatgaaga tggaagtgtg ggatacgtac ccgacagcct gacttccgaa tttctgacaa
     2881 aaaatggtcg aaaagttcgc gacggtggcg gaattatccc cgacaaacag accgaaagta
     2941 aaggaaaatt gaacgttgcc cactatattt ttgcacaaaa cctgtatttt gattttgcga
     3001 cccgctacgc cagtttacat cctactattg cgtctccaaa ggattttaaa ctttctgaag
     3061 acgactttaa caattttgtg gattacctgc acgaaaagaa tttcacttac tcatatcaga
     3121 ccaataaata ttttcagcaa ctctatcagg ttgccaaaat agaaggctta gattcaatag
     3181 cacaatctga gtttgaagca ttaaaaacca aattaacacc caatgtagat gccgatattc
     3241 gggaaaacaa ggatgaaatt gaggcattgc tcagtctgga aataattaaa agatactatt
     3301 atcaaaaagg acaaattgag ttttcacttc gaactgatga cgatttgaaa gaagcaattc
     3361 aaattttaaa gtccgaagaa acctatcgaa aaattttatc ggccatttaa aaaaacagtt
     3421 gatattttat aagttaagta aaggtttttg attggaaata aaatattttt ccttcaaaaa
     3481 ccttcatttt ataaattttt atcgtacttt tgttcccgtt ttaaatatgg cagttataac
     3541 accttttatc atggagaaaa aaaatatcgg agaaaaaatt cttgcccttt gtaagagtag
     3601 aaatctaagc attcaagatt tggttgacac cacaggttta tctgaagttc aaattaacag
     3661 agtgatcgaa agcgaaacta ttccttcgct ggccccactt ataaaaattg cacgcgcatt
     3721 gggcgttcgg ttgggaactt ttcttgatga cgcagaagaa ttgggacctg ttgtccatcg
     3781 ttccggcgaa gttcagcaac cggcttcttt ttccagtcag ctttcggatg ccaattctca
     3841 tctcgatttt tatgcgttgg catcacgcaa aaccaaccga cacatggaac ccttcttcat
     3901 cgatattcag ccttcatcga cacacgaacc cattttttct tcccacgaag gagaagaatt
     3961 tatttttgtt ctggaaggag cagcaaaaat agattatgga aaccaaactt ttttcctcca
     4021 tcccggcgac agcatttact acgactctat tatcaaccat ctcgtcagct cggctgatga
     4081 aaaaccggcc aaaattctgg ctgtcgttta cacacctctt taatcttttc accttgttga
     4141 attattgaga attttattgc tgttttttcc ggtctgaaaa aattttattt ccatcaagca
     4201 ccataaagct ttatacaaat cttatcatgg aattatttga aaaaaccata ggcgaatggc
     4261 tcgaatattg ggcaaatgaa actcccgagc gtgaatacct tgtttatgcc gatcgaaatt
     4321 tgcgtttcac atggaaaagt tttaacgaac gtgtggacaa tatggccaaa ggtttaatgg
     4381 ccatcgatgt aaaaaaaggg gatcatgtcg gtattttcgc tcaaaacgtg cccgactggt
     4441 tgacttttct ttatgcctgc gccaaaattg gagcaatagc cgtaacactc aataccagtt
     4501 gcaaagagca cgaacttgcc tatattattg agaattcgga catgcataca ttgtgtatga
     4561 cagaaggcga caacggaaat gactttatta aaaccatcta ttcgttgatt cctgagctaa
     4621 aaacttcgga acgcggaaga ctgaaatcaa accgctttcc tgttttgaaa aatgttgtct
     4681 ttatcggaca ggaaaaacac ag
//
LOCUS       DAOTAX010000107         4521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_15740_length_4521_cov_21.020152, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000107 DAOTAX010000000
VERSION     DAOTAX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15740_length_4521_cov_21.020152"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..3294)
                     /locus_tag="PLM04_08215"
     CDS             complement(187..3294)
                     /locus_tag="PLM04_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HON03091.1"
                     /translation="MTLKKIQIAIFLCISSSFSLFAQTPDWENPRVFGVNNEPTRASF
                     LPYQSESMAIEDDYLRSSYYMLLDGNWKFNFSPRPDLRPVDFYNQEYNVSGWKEIKVP
                     GNWELQGYGKPIYTNIIYPHPKNPPYIPHTDNPVGSYRRDFHLPESWDGRRVFLHFEN
                     GTAAMYVWINGKKVGYNQGAKSPVEFDITNYVQKGKNSIACEVYRWSDGSYLEDQDFW
                     RLSGFERSIYLYSTDQSRIFDFFVKTDLDKAYKNAMLSLDIKLKNYTNTKKKQIVEIK
                     LLDSNMKAVFTQQKVVQIEGENSVESSFVQKVSAPKLWTAETPNLYTLVLTLKNENGK
                     AIEFTSTKVGFRKIEIKDGQLLVNGKRVLFKGVNIHEHNHLTGHTINREIMMQDIRLM
                     KQFNINAVRTSHYPQPVLWYKLCDEYGIYLIDEANIESHGMGYGKESITHFPEWWDAH
                     LDRTIRLVERDKNHPSVIVWSLGNESANGKAFEITYDWVKNRDNTRPVQYEQAHEERN
                     TDIICPMYPDFEHMRKFASRKLDRPYIMCEYAHAMGNSIGNFQEYWDIIRSSKHFQGG
                     YIWDWVDQGLLQKDENGREYWAYGGDFGVGFEYHNDANFCANGLVAPDRTPHPHLYEV
                     KKVYQNVLINAKDLSKGIVTVTNDYRFTNLKDILYFEWHLLKNGVKVSQGKFDVDLAP
                     EASKDVRLALPELKKQQGDEYFLQIYGYTLNKTELIPANFELVREEFALSGNDYFTLT
                     KLSTSKPQFVAKDNVIHVTAGDLLAEFHKSVAGLVELSYKGIQLLKGKSPELNFWRAP
                     TDNDWGEGAHKKMNIWRNAGNSVVLKDVVLVEGENKLQINYHLFSTDIQSDIFINYTV
                     SGDGSIVCDIEYKTQNNFLPEMPRFGIMFTLPEEFENLSWYGRGPWENYIDRNVSSFM
                     GIYESKVKDQYYPYVRPQENGNKTDIRWITLTNNEGVGLKVEGLQPISFSALNYRPED
                     FDPGFSKKQQHISDMYPRREVVLSVDLFQRGLGGLNSWGAGPLKQYRFTEKIYSYSFK
                     ISGVE"
     gene            complement(3365..4426)
                     /locus_tag="PLM04_08220"
     CDS             complement(3365..4426)
                     /locus_tag="PLM04_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03092.1"
                     /translation="MKKNYLVLLALLFIGGFIRLLAENPEYVLQKQQPGKKYLNAFYY
                     RAHMYTMVPRQIREDMKWMARAGTNAITISVLEQDFDAADENIRFIISEANKQGMEVF
                     AAPSRWGGIVAGSPKVPSLFTIRNPQTWVLRKDGTAVDSSVSGRISSINSEETYRFVE
                     SLLDKLFRKWNFKGVIWDEPKTLSLDYSPAAIQKFGNNPTMDQQIQANVDFYSRLNKY
                     VKTKYPDKTTCLFIYSSYEDEIINKMAAIEYLDYFGADGRPWHSADGGQLEGNGKTLL
                     GTQGQRFIDAAHANNKKSLFLMENHNLKKADIHLLEKGMPQLLQMDVDQLIYYYYPRN
                     VEEPDKTMEIIKWNLKKYR"
BASE COUNT         1308 a          989 c          776 g         1448 t
ORIGIN      
        1 attatgtgca attaattatt agaaattata taataagtta gttgattaat ctataaagat
       61 acttattttc agtgaattaa ccaaataaaa attgaaataa tttattgatt ttcaattatt
      121 taattaaaaa tataacacac tattgaacat cttataaatt ttaaataatg caaattctta
      181 tggcatttat tctaccccag aaattttaaa ggagtatgaa taaatttttt cagtaaaccg
      241 atactgtttc aatgggcctg ctccccatga atttaatcca cccaaacccc gttgaaacaa
      301 atcgactgac aaaaccactt cgcggcgcgg atacatatcg ctgatatgct gttgtttttt
      361 cgaaaacccg ggatcgaagt cttccggacg ataatttaaa gcactgaatg aaatgggctg
      421 aagaccttct accttcaatc caaccccttc gttattggta agtgttatcc aacggatatc
      481 agttttattg ccattttcct gtggacgaac atagggatag tattgatctt ttactttact
      541 ttcatatatt cccataaaac tcgaaacatt gcggtcgata taattttccc atggtccgcg
      601 tccataccaa ctcagatttt caaattcttc gggcaaggtg aacatgattc caaaacgggg
      661 catttcaggt aaaaagttat tttgagtttt atattctata tcacaaacaa tacttccatc
      721 accggaaacg gtataattaa tgaaaatatc gctttgaata tcggttgaaa acagatgata
      781 gttaatctgt aatttatttt ctccttcgac taaaactaca tctttgagta ccacgctatt
      841 gcctgcattg cgccaaatat tcattttttt gtgagcacct tcgccccaat cgttatctgt
      901 aggagcacgc cagaaattta gttccggtga ttttcctttt aacaactgaa ttcctttata
      961 tgacaattcg accaaacctg caaccgattt atgaaattca gccaaaagat ctccggccgt
     1021 aacatgaatc acgttgtctt ttgcaacaaa ttgaggtttt gaagtcgaaa gttttgtaag
     1081 agtaaaataa tcattccccg ataaagcaaa ttcctcccga accaattcaa aatttgcagg
     1141 aatcagttct gttttattta aggtgtagcc ataaatctga aggaaatatt catcaccctg
     1201 ctgctttttt aattcaggta aagccaatct gacatctttc gatgcttcgg gagcaagatc
     1261 aacatcaaat ttcccctgcg atacttttac tccgtttttg agtaaatgcc attcgaaata
     1321 caaaatatct ttcaggttag taaacctgta atcgttcgtt acagttacaa ttcctttcga
     1381 caaatcctta gcattgataa gcacattttg atatactttt ttcacttcgt acagatgtgg
     1441 gtgaggagta cgatcggggg caaccaagcc gttggcacag aagttagcat cgttgtgata
     1501 ttcgaatcca acaccaaaat cgcctccata agcccaatat tcccgtccat tctcatcttt
     1561 ttgcaacaat ccctggtcaa cccagtccca gatatagcca ccctgaaaat gtttactgct
     1621 tcgaatgata tcccagtatt cctgaaaatt ccctatactg ttgcccatgg cgtgagcata
     1681 ttcgcacata atgtaaggac gatcgagttt acgggatgca aatttcctca tgtgttcaaa
     1741 gtccggatac atgggacaaa taatatcagt attacgttct tcgtgagctt gctcgtattg
     1801 taccgggcgg gtattatccc tgtttttaac ccaatcgtaa gttatttcaa aggcttttcc
     1861 attggccgat tcgttgccca acgaccacac aataaccgat gggtgatttt tgtcgcgttc
     1921 aaccaggcga attgtacgat ccaaatgcgc atcccaccat tcgggaaaat gtgtaattga
     1981 ttcttttcca taacccattc cgtgagactc aatattggct tcatcaatca gataaattcc
     2041 atattcgtcg cacagtttgt accaaagcac aggctgagga taatgactcg tacgaacagc
     2101 attgatatta aactgcttca tcagccggat atcctgcatc ataatttctc tgtttatagt
     2161 atgtccggtt aaatggttgt gctcatgaat attcactcct ttaaacaaaa cacgttttcc
     2221 atttaccagc aactgaccgt ctttgatttc aattttccgg aaaccaacct tggtcgatgt
     2281 aaattctatc gcttttccat tctcattttt taaagtcaac accaatgtgt acaaatttgg
     2341 tgtttcagcc gtccagagtt ttggtgcact tactttttgc acaaaactgg attcaaccga
     2401 attttcaccc tctatctgaa ccaccttttg ttgcgtgaaa accgctttca tattggaatc
     2461 caacaactta atttcaacaa tttgcttttt cttcgtattg gtatagtttt tcaacttaat
     2521 atccaatgat agcattgcat ttttatatgc cttatccaaa tctgttttaa cgaaaaagtc
     2581 aaaaatgcgg ctctgatcag tgctatacaa ataaatactt ctttcaaagc ccgataaccg
     2641 ccagaaatcc tgatcttcga gatacgatcc atcgctccag cgatatactt cgcaggcgat
     2701 tgaatttttg cctttttgca cataattggt tatatcgaat tcaaccggac tttttgctcc
     2761 ctgattatat cccacctttt tgccattaat ccaaacatac attgccgcag ttccattttc
     2821 gaaatgcaga aagacccgtc gtccatccca actttcaggc aaatgaaaat cccgtcggta
     2881 tgatcccaca ggattatcgg tgtgcggaat atacggagga tttttggggt gcggataaat
     2941 aatgttggta taaataggtt taccataccc ttgcaattcc cagtttcccg gaacttttat
     3001 ctctttccaa ccggacacat tgtattcctg gttataaaaa tcgacagggc gcaggtcggg
     3061 acgtggcgaa aagttgaact tccagttgcc atccaacaac atatagtacg atgaacgcaa
     3121 atagtcatct tcaattgcca tactttcgct ttgatatgga agaaaacttg ccctggtggg
     3181 ttcattattc actccaaaaa ctcgcggatt ttcccaatcg ggggtttgtg caaaaagaga
     3241 aaaagaactt gagatgcaca agaaaatagc aatctgaatt ttttttaatg tcatgttata
     3301 tgatttttaa tttttttatt gtccggtaaa agcttgcatt gatctgacta agaccttatg
     3361 ttccttatct gtacttcttt aaattccatt ttattatttc cattgtttta tcaggctcct
     3421 caacattccg cggataataa taatagatga gttgatccac atccatttgc agcagctgtg
     3481 gcattccttt ttccagcaaa tgaatatctg ctttctttaa attatgattc tccatcagga
     3541 aaagtgactt tttattgttg gcatgtgcag catctataaa acgttgtccc tgcgttccca
     3601 gtaaggtttt cccattacct tcgagttgac cgccatccgc cgagtgccaa ggccttccat
     3661 cggctccaaa atagtcaaga tactcaatcg cggccatttt attgattatc tcgtcctcat
     3721 aactggaata aatgaacaaa caggtggtct tatcgggata tttggttttc acatacttat
     3781 tcagccggga ataaaaatcc acattagcct ggatttgctg gtccatggtc gggttattcc
     3841 cgaacttctg gattgcagcg ggcgagtagt ccaggctcaa tgttttgggt tcgtcccata
     3901 tcacgccctt aaagttccat ttccggaata acttatccaa taagctttcg acaaaccgat
     3961 aggtttcttc gctgtttata ctgcttatcc tgcccgaaac tgaagagtcg accgcagtac
     4021 catctttgcg caaaacccat gtctgtggat tacgaatggt aaaaagactg ggaacctttg
     4081 gcgagccggc aactatccct ccccaccgtg aaggcgctgc gaatacctcc atgccctgct
     4141 tgttggcctc acttataata aatcgtatat tctcatcagc agcatcaaaa tcctgttcaa
     4201 gcaccgatat cgtgatggca ttcgttccgg cacgagccat ccatttcata tcttccctaa
     4261 tctggcgagg aaccatggtg tacatatgcg ccctgtaata gaatgcattc aggtactttt
     4321 tacccggctg ttgtttctgc agcacgtatt cggggttttc agcaagtaat ctaatgaaac
     4381 cacctataaa aagtaatgcc agtaagacta agtaattctt tttcatccct aaataattat
     4441 taaattaaac aatatcagca tattgaaaaa acagataagg agttaaaatt tcatccgcga
     4501 actcgaggtt catgtgtttg t
//
LOCUS       DAOTAX010000108         4496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_15848_length_4496_cov_26.762666, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000108 DAOTAX010000000
VERSION     DAOTAX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15848_length_4496_cov_26.762666"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..2054)
                     /locus_tag="PLM04_08225"
     CDS             complement(216..2054)
                     /locus_tag="PLM04_08225"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HON03093.1"
                     /translation="MKATITGQAQQIVQTRKEKSGRLQGDNPKIGIRPTIDGRQGGVR
                     ESLEDQTMRMALNLAKFLSSNLKYSDGTPVECVIADTTIGRVDEAAACAEKFKKEGVS
                     ITITVTPCWCYGSETIDMDPHTIKAVWGFNGTERPGAVYLAAALAGYNQKGLPAFGIY
                     GRDVQDAGDTSIPADVQEKLLRFSKAAIAAASMKGKSYLSIGSVSMGIAGSIVDPNFF
                     QEYLGMRNEYVDMSEINRRVQLGIFDQEEFARAMEWTSKYCKVNEGEDKNDEDKKKTR
                     EQLDKEWEFVVKMTIIIRDLMIGNPKLREAGFLEEALGHNAIASGFQGQRQWTDFMPN
                     GDFSEAILNTSFDWNGIREPFVVATENDSLNGVAMLFGKLLTNTAQVFADVRTYWSPE
                     AVERVTGYKLEGIASNGLIHLINSGSAALDGSGQQTKDGKPAMKPFWEITDEEVQKML
                     DATTWYPADCGYFRGGGFSSKFVTKGGMPVTMARVNLVKGLGPVLQLAEGWTVEIPAE
                     VHKILDDRTDKTWPTTWFVPRLTGKGNFKDVYSVMNNWGANHGAFSYGHIGADLITLA
                     SILRIPVCMHNVEEDKIFRPSAWAAFGMDAEGSDYRACATYGAMYK"
     gene            complement(2177..>3174)
                     /locus_tag="PLM04_08230"
     CDS             complement(2177..>3174)
                     /locus_tag="PLM04_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HON03094.1"
                     /translation="KRIIMKVNFSHSKSKINTLIDAISNDIASGVLKLGDNLPSINET
                     SLKFKISRDTVFKAYKELKRRNLIDSNPMKGYFVKGEVNHVLLLLDTYSPFKQNLYNR
                     FAQNLPKSWKVDLIFHQYNKNLFDTIIRESIGRYSMYVVMNFSNTEFSESLKSIPEGK
                     LLLLDFGDFDKKQYPYLCQNFDQALYDCLSEQKPIISKYKKIVMIFPTENNHPKSSIK
                     FVKEFCSDSNIAFELIEDIYLLNNIEENVLYFIMSHTDLVSIILASKNKNLKIGQDIG
                     ILVYNDEPLLEVIQEGIAAISIDFGLLGEKAAQFIKDKMFVQEFLPTRFIFRPSI"
     assembly_gap    3175..3274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3275..4383)
                     /gene="fucP"
                     /locus_tag="PLM04_08235"
     CDS             complement(<3275..4383)
                     /gene="fucP"
                     /locus_tag="PLM04_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HON03095.1"
                     /translation="MKSDTKIPLLKGSDGTNYLIPFILVTSLFFFWGVANNMTDTLLS
                     AFKNIMEMSDFQTSFIQMAFYGSYACFAIPAALLIRKYTFKHGIVVGLSLYVVGTFLF
                     YPAAQLASYGFYLFALYVLAGGCSILETAANPYILTMGRKENMTRRLNFAQSFNPIGS
                     ISGIIISQIFILSQLSQNKSTMNDAELLAVQHKELSAVTLTYMGLGAVLVALLLIIIF
                     YKKMPATKDTTGLSFGATVGKLLKRKHYTLGVVAQFFYVGAQIGVWSYTIRYVMLALG
                     LVDPATSEIFPAKVQGFIAEHNLWSSIWQLDENSTAENIGNSFYLFSLILFTVSRFIF
                     TALMRYFRPGILLSVAATGAIISAAIAIFATGMTGV"
BASE COUNT         1360 a          878 c          756 g         1402 t
ORIGIN      
        1 atatgaattt tcaagaacaa catttttaaa aactttcgta cggcagtgat aaaaaatgtg
       61 ggttaaggtt tgactaactt aagaataatt atccaactaa tataaaaaga tttgaaggca
      121 acaataatta atagtatgcc ttcaaatcag atattaattt tttctcagaa attgattgat
      181 taattatctg agactccctt tagggttggg gtcatttact tatacattgc tccgtaagtt
      241 gcacatgccc gatagtctga accttcggca tccatcccga atgcagccca tgcgcttggt
      301 cggaaaattt tatcttcttc cacattgtgc atgcaaacag gaatacgtaa tatcgaagcc
      361 agtgtaatta aatcagcgcc gatatgtccg taactgaaag cgccgtgatt tgcaccccag
      421 ttgttcatta ccgaataaac atccttgaaa tttcctttac cggtcaatcg aggtacaaac
      481 caagttgtag gccatgtttt atcggtacgg tcatctaaga ttttgtgaac ttcggcagga
      541 atttccaccg tccaaccttc agccaattga agtacaggac caagaccttt cacaagattt
      601 acacgagcca tagttaccgg cattccgcct tttgttacaa attttgaaga aaatcctccg
      661 ccacggaaat aaccacaatc tgcaggatac caggttgtag catctaacat tttctgaact
      721 tcttcatcgg ttatttccca gaaaggtttc atggcaggtt ttccatcttt ggtttgctgt
      781 ccgctgccat ctaaagccgc cgagcctgaa ttaatcaggt gaattaaacc gttcgatgcg
      841 ataccttcca gcttatatcc ggttacccgt tcaacagctt caggactcca gtacgtacga
      901 acatcggcaa atacctgtgc tgtattggta agtaattttc caaaaagcat ggctacgccg
      961 ttcagtgagt cgttttcagt agctactaca aaaggttcgc gaataccatt ccagtcgaat
     1021 gaagtattca gtatcgcttc tgagaaatct ccattgggca taaaatctgt ccattgacgt
     1081 tgaccctgaa aacctgaagc aatggcattg tgtcctaaag cttcttcgag aaatcctgct
     1141 tcacgcagtt tcggattgcc aatcatcaaa tcgcggataa taatggtcat ttttacaaca
     1201 aattcccatt ctttatccaa ttgctcgcgt gttttctttt tatcctcatc gtttttatct
     1261 tctccttcat ttactttgca gtattttgat gtccattcca tggctcttgc aaattcttcc
     1321 tgatcgaaaa ttcccagttg aacccgacgg ttaatttcgc tcatatcaac atattcattg
     1381 cgcatgccaa gatattcttg aaagaaattg ggatcgacaa ttgaacctgc aattcccatc
     1441 gaaaccgaac cgatagacaa ataagatttt cctttcatcg aagctgcagc aatggcggct
     1501 tttgaaaaac gtagtaattt ttcctgaaca tctgcaggaa ttgaagtgtc accagcatcc
     1561 tgcacatcac gtccgtaaat accaaatgca ggtagtcctt tttggttata accggctaaa
     1621 gcagctgcca gatatacagc tcccggacgt tctgttccgt tgaaacccca gacggcttta
     1681 atagtgtgcg gatccatatc gatggtttcg ctgccataac accaacaggg cgttacagtt
     1741 atggtaattg aaacaccttc ttttttaaat ttttcggcac aagcggcggc ttcgtccact
     1801 cttccgattg tagtgtcggc aataacacat tcaaccggag tgccatcgct gtattttaag
     1861 tttgacgata aaaatttagc aagattcaat gccattctca tagtctgatc ttcgagcgat
     1921 tcacgcactc cgccctgccg accatcaatt gttggacgaa ttcctatttt aggattatcg
     1981 ccctgtagac gtcctgattt ctcttttctg gtttgcacaa tctgttgcgc ttgccctgta
     2041 attgttgctt tcattctgat attttttatt tgatttttaa atatattgtt tggtacttat
     2101 ataaaatggt aaatggtaaa tggtaaatca ttaaattctt ctgtcctatt ctatgcagat
     2161 tatttgctaa aaaaaattat atcgatggtc tgaaaataaa tcttgtaggt aaaaattcct
     2221 gaacgaacat tttgtctttt ataaactgag ctgctttttc gcccagcaat ccaaaatcaa
     2281 tcgaaatagc ggcaattcct tcctgaatta cctccaaaag aggttcatcg ttatacacca
     2341 aaataccaat atcctgtcct attttcagat ttttattttt acttgctaaa attatgctca
     2401 ccaaatcggt atgcgacatt ataaagtaca atacattttc ttctatattg tttaaaagat
     2461 aaatatcctc gattaattca aatgctatat ttgagtctga acaaaattct ttcacaaatt
     2521 tgattgaact tttaggatga ttgttttcag ttggaaaaat cattacaatt ttcttgtact
     2581 ttgaaattat tggcttttgt tcagataaac aatcatataa agcttgatca aaattctgac
     2641 ataaatacgg atactgtttt ttatcgaaat ctccaaaatc aagcaatagc aattttcctt
     2701 cgggtatgga tttaagtgat tcagaaaatt cagtattcga aaaattcatc accacataca
     2761 tgctgtaacg tccgatgctt tccctgataa ttgtatcaaa taaatttttg ttatactgat
     2821 gaaaaattaa atcaactttc cacgatttgg gtaaattctg tgcaaaacga ttgtatagat
     2881 tttgtttaaa aggtgaatat gtatcgagca gcaataaaac atgatttact tcacccttta
     2941 caaaataacc tttcatcggg ttcgaatcaa tcagatttct tctttttaat tctttatacg
     3001 ccttgaaaac cgtgtctcga gaaattttaa actttaagct cgtttcattg atcgatggta
     3061 aattatctcc caacttcaac acacccgatg caatatcatt cgatattgca tcaatcagcg
     3121 tgttgatttt cgatttagag tgagagaaat ttactttcat tattattctc tttannnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacacct gtcattccgg ttgcaaaaat
     3301 tgcgattgca gcagaaatta ttgcacctgt cgcagctact gacagtaaaa ttccgggacg
     3361 gaaataacgc attaaagcag tgaaaataaa tcttgaaaca gtgaataaaa tcagcgagaa
     3421 aagataaaag gaattgccaa tattttcggc agtagagttt tcatcgagtt gccagatact
     3481 gctccataaa ttatgttcag caataaatcc ctgcactttt gccggaaaaa tttcagaagt
     3541 tgcaggatcg acaagcccga gtgcaagcat tacgtaacga atggtgtaag accaaacgcc
     3601 gatttgagct ccgacataaa aaaactgagc aaccacacca agtgtataat gttttctttt
     3661 tagcaatttg ccaactgtag cgccaaaact taatcctgta gtatctttgg tggcaggcat
     3721 ttttttgtaa aaaattataa ttagaagtaa tgctacaaga actgcaccca agcccatata
     3781 cgtaagtgta actgctgata attctttgtg ctgaacggca agcaattcgg catcgttcat
     3841 tgtgctttta ttctggctta actgtgataa aataaatatc tgactgatga taattcctga
     3901 aattgaacca atcggattaa aagattgggc aaaatttaac ctgcgggtca tgttttcttt
     3961 ccgacccatg gttaaaatat aaggattggc agcagtttcg aggattgagc aacctccggc
     4021 taaaacatac aatgcaaaca aataaaagcc gtaggatgca agttgcgcag ccggataaaa
     4081 caggaaagtt ccaaccacat aaagcgagag ccctacaact attccatgtt taaatgtata
     4141 ttttctgatt agcaaagctg ccggtatcgc aaaacatgca taagagccat aaaatgccat
     4201 ttgtatgaac gatgtctgaa aatcggacat ctccataatg tttttaaacg cagacagaag
     4261 tgtgtcagtc atgttgttag caacccccca aaaaaagaaa agagaagtaa ctaaaataaa
     4321 aggaattaaa taatttgtac catcgctgcc tttcaataaa ggaattttag tgtctgattt
     4381 catgatattt tatttgtttg tattttttga tacagacaca acacattgca tctgtgcatg
     4441 ttttacattt ttgtctataa tttgtaaata gtaaatgatt aaatggtaaa tggtaa
//
LOCUS       DAOTAX010000109         4423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_16148_length_4423_cov_30.433150, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000109 DAOTAX010000000
VERSION     DAOTAX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16148_length_4423_cov_30.433150"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1184)
                     /gene="wecB"
                     /locus_tag="PLM04_08240"
     CDS             complement(114..1184)
                     /gene="wecB"
                     /locus_tag="PLM04_08240"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HON03096.1"
                     /translation="MRKIVTVVGARPQFIKAATLSRQFPKVGVEEIIIHTGQHFDNNM
                     SDVFFEEMEIPRPAYHLEIHNLTHGAMTGRMLEGIEKILLKEKPDGMLVYGDTNSTLA
                     GALAAAKLNIPVIHVEAGLRSFNMEMPEEINRILTDRVSSLLFCPTDVAVENLKKEGF
                     EHFPARIIKNGDVMQDAALYYASKAEEKSDILQKIGGEKFVLATIHRQENTDHPEKLR
                     NIVEGLNQIHRQVRVIIPLHPRTRKILQQLSIIPNFMIIEPVGYFDMIVLEKSCELII
                     TDSGGVQKEAFFFGKHCITLREQTEWTELVENGFNILVGSDVEKLLQAFATFQNKKSD
                     FSIDLYGNGHAAEKAAKDIAAF"
     gene            complement(1172..2473)
                     /locus_tag="PLM04_08245"
     CDS             complement(1172..2473)
                     /locus_tag="PLM04_08245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON03097.1"
                     /translation="MKKVLIITYYWVPSGGAGVQRWVKFAKYLRSFGWEPVVYTPQNP
                     EYPALDHSLEKDVPPDMEVIKTPIWEPYNIYRNLMGKKNEPINAGFISENKKSGWKEQ
                     FSIWIRGNFFIPDPRRFWVKPSVKFLKNYISENQIDAVVTTGPPQSMHLIGLGLKKNF
                     PTLTWLADFRDPWTHIDFYDDLYLTKIADRIHHRLEKKVVSSADAVVVVSKSMKEEFL
                     RLQPKRIEMIPNGFDEEDFRTNKTELDEKFTISHIGTLNAARNPTIFWKVLNELCAEN
                     EQFKSDLLIQLIGKVDFSILEEINENNLKDNFLKIDYLPHNEAIAKQMSSQVLLLLIN
                     QTNNAKGIVTGKLYEYLAARRPILGIGPTNGEVGEILKSSQAGIMIDYIDAKKMKATI
                     LDYYQQYKNHSLTVPSNLVENFSRKNLTRDLANLLDSLCEK"
     gene            complement(2533..4059)
                     /gene="gltX"
                     /locus_tag="PLM04_08250"
     CDS             complement(2533..4059)
                     /gene="gltX"
                     /locus_tag="PLM04_08250"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HON03098.1"
                     /translation="MRRVRVRFAPSPTGPLHIGGVRTALYNFLFARKNNGDMLLRIED
                     TDSSRFVPGAEDYIVESLKWLGITIDEGIGAEKEGAFGPYRQSERKHIYRKYVDQLLN
                     DGKAYIAFDSPIELESKRAEIPNFQYDAQTRLSMNNSLTLPEEEVRKRLENDEQYVVR
                     IKIEPGEDIVVNDLIRGEVSINSSVIDDKVIFKSVDELPTYHLANVVDDYLMQITHVI
                     RGEEWLPSSPLHVLLYRYLGWENEMPQFAHLPLLLKPDGKGKLSKRDGDRLGFPVFPL
                     NWKDPRTGEIYNGFREAGYFPEAVVNFLALLGWNPGTDQEIFSMDELIEQFSLERVSK
                     SGAKFDYEKAKWFNHKYLQMKPDEEVADIFQRILIEKGFIEDTDKVIKIISLIKDRAD
                     FITDLWEQSHYFFEAPEHYDAKAVQKRWKPEIPEQLEELGKMLLSLEAASADEMEKSI
                     KEWISGKNYHLGNLMNALRLALVGELKGPSIFEIVEVLGKEEAARRIKKAIEVLSGES
                     "
BASE COUNT         1334 a          936 c          766 g         1387 t
ORIGIN      
        1 ctttaccttt tatttcccct gaaaaagttg cattaattac ttgaatttac gcttttttaa
       61 aagatgaagc agataaaatc aaaaaaagat gatattgcaa aacaatataa cattcaaaaa
      121 gcagcaatat cttttgctgc cttttcggct gcatgaccat tgccatacaa atctatggaa
      181 aaatcagatt ttttattttg aaaagtcgca aaagcttgca aaagtttttc tacatcgcta
      241 cctaccaaga tattaaatcc attttctacc agctcagtcc actcggtttg ctcgcgaagc
      301 gtgatacaat gtttaccaaa gaagaaagct tccttctgca caccaccgct atcggtgata
      361 atcagttcgc acgatttttc gagcacaatc atgtcaaaat aaccgaccgg ctctattatc
      421 ataaagttag gaataatgct caattgttgt aaaattttgc gggtacgtgg atgcaaagga
      481 ataattacac gtacttgacg atggatttga tttagtccct cgacaatatt tctcaatttt
      541 tcgggatgat cggtattttc ctgacggtga atggtagcca gcacaaattt ttccccaccg
      601 attttctgta aaatatcgga tttttcttct gcttttgaag cataatacaa agctgcatcc
      661 tgcatgacgt caccgttttt tataattcgt gcaggaaaat gctcaaagcc ttcttttttc
      721 aaattctcaa cagccacatc cgttgggcaa aaaagcaggc tggaaacacg atccgtaaga
      781 atacggttga tttcttccgg catttccatg ttgaacgaac gcaaaccggc ttctacatga
      841 atcacgggaa tgttcaactt agcagcagcc aatgcgccgg caagcgttga attggtatca
      901 ccgtaaacca acattccatc aggtttttct ttcaacagaa ttttttcaat tccttccagc
      961 attcggccgg tcatggctcc atgtgtcaga ttgtgaatct ccagatgata cgccggacgc
     1021 ggaatttcca tctcctcaaa aaaaacatcg gacatgttgt tgtcgaaatg ctgtccggta
     1081 tggataatta tttcttccac gccaactttt ggaaattggc gactcaacgt ggctgctttg
     1141 ataaattgtg gacgagctcc aacaactgta actatttttc gcataatgaa tccaataagt
     1201 ttgctaaatc acgagtcaaa ttttttcgag agaaattttc cactaaattg cttggaactg
     1261 ttaatgaatg atttttatat tgctgataat agtcaagaat tgttgccttc atttttttag
     1321 catcgatata atcaatcatg atgccagctt gagagctctt caaaatttca ccaacttctc
     1381 catttgttgg tccaattccc aaaatggggc gacgagcagc cagatattca tataatttgc
     1441 ccgtaacaat tccctttgca ttgtttgttt gattaatcaa caacaaaagc acctgagatg
     1501 acatttgttt tgcaattgct tcattgtgag gcaaataatc aatctttaaa aaattatctt
     1561 ttaaattatt ttcattgatt tcttctaaaa tagaaaaatc tactttgcca attagttgaa
     1621 tgagtaagtc ggacttaaat tgttcatttt cagcgcaaag ttcgttcaaa actttccaga
     1681 atattgttgg atttctggca gcattcaggg tgccaatatg cgaaatggtg aatttctcgt
     1741 ccaattctgt tttgttagta cgaaaatctt cctcatcaaa accattggga atcatttcaa
     1801 ttcttttggg ctgtaacctt aaaaattctt ccttcatgct cttggaaacc acgacaactg
     1861 catcggccga actgaccact tttttttcca atcgatgatg gatgcggtcg gcaattttcg
     1921 ttaaatacaa atcgtcataa aaatcgatat gggtccaggg atcacgaaaa tcggccagcc
     1981 acgttaaagt tggaaaattt tttttcaatc ccagtccaat caaatgcatg gattgaggag
     2041 gtccggtagt aacaaccgca tcaatctggt tttcggaaat atagtttttt aaaaatttca
     2101 ccgaaggttt tacccaaaag cgacgtgggt cgggaataaa aaaattgcct cgaatccaaa
     2161 tggaaaattg ttccttccag cccgattttt tgttttcgga aataaatccg gcattgatcg
     2221 gctcattttt cttgcccatc aagtttcgat aaatattgta aggttcccaa ataggcgttt
     2281 taatcacttc catatcgggc ggaacatctt tttcgagcga atggtcaagc gcaggatatt
     2341 ccggattttg aggcgtatat acaaccggtt cccaaccaaa acttcgcagg tactttgcaa
     2401 acttcaccca tcgctgcacg ccggcacctc cactcggtac ccaataataa gtaatgatca
     2461 gtactttttt cattcgttat tttaagaacc tttttactat gagagctttt ttagctaaaa
     2521 aacttccgtt tattaacttt caccgctcaa cacttcgatg gctttcttaa ttcgtctggc
     2581 cgcctcttct tttcccagca cttcaacaat ttcaaaaatg gacgggccct tcaattcccc
     2641 taccaaagcc agacgaagag cattcattaa attgccgaga tggtaatttt ttcccgaaat
     2701 ccattcctta atgctttttt ccatttcatc agccgaagca gcttccagcg atagcaacat
     2761 tttacccagt tcttcgagtt gttcaggaat ttcgggtttc cagcgctttt gcacggcttt
     2821 ggcatcataa tgttccggtg cttcaaaaaa gtaatgagac tgttcccaca aatcggtaat
     2881 aaaatcggct cgatccttga tcagagagat aattttaatc actttgtcgg tatcctctat
     2941 aaaacctttt tcaatgagaa tacgttgaaa aatatccgcc acctcttcat ccggtttcat
     3001 ttgcagatat ttatgattga accatttggc tttttcataa tcgaattttg caccactttt
     3061 gctgacacgc tctagcgaaa actgttcaat gagttcatcc attgaaaaaa tttcctgatc
     3121 ggtaccggga ttccagccca gcaaagccaa aaaatttact accgcttcag gaaaatatcc
     3181 ggcttcgcga aaaccattgt agatttcgcc ggtgcgggga tctttccaat tgagaggaaa
     3241 aaccgggaac ccaagcctgt cgccgtcgcg tttactgagt tttccctttc catcgggttt
     3301 aagcagcaaa ggaaggtgag caaattgcgg catttcgttt tcccacccaa gatatcgata
     3361 cagcaaaaca tgcaaaggcg aagacggtaa ccattcttct ccacgaatta cgtgagtgat
     3421 ctgcatcagg taatcgtcca caacattggc caaatgatag gttggcaatt catcaaccga
     3481 tttaaaaata actttgtcgt caatcaccga agaattaatg ctgacttcac cacgaatcaa
     3541 atcattcact acaatatctt ctccgggttc aattttgatg cgcaccacat attgttcatc
     3601 gttctccaat ctttttctga cttcctcttc gggcaaagtc agagaattgt tcatcgaaag
     3661 acgtgtctgt gcatcgtact gaaaattggg tatttcagcc cttttacttt ccagttcaat
     3721 gggcgaatca aaagcaatgt aggcttttcc gtcatttaaa agctgatcta cgtattttcg
     3781 gtaaatatgt tttctttcgc tttgccgata agggccaaat gcgccttcct tctctgcccc
     3841 aataccctca tcaatagtta ttcccaacca tttcagcgac tctacaatat aatcttcagc
     3901 tccgggaaca aagcgtgaag aatcggtatc ttcaatgcgc aaaagcatgt cgccattgtt
     3961 ttttcgggca aaaagaaaat tgtacaaagc cgtacgaaca ccaccaatgt gcaaaggccc
     4021 cgtaggactt ggcgcaaaac gaactctcac tcttctcatg aaaaggaaat ttaatgtttg
     4081 aagctttaaa actgatatat gttatcggtt tgcaaaaata agaaatttaa ctgagatata
     4141 aacttaaaat gttcattagg agtttaagga aaataattat ttttaaatcc aactcaaaat
     4201 ctaagttttc attaacaacc ttgagaaact ttacatctaa aattaattga tgagggaatt
     4261 aattaaaaaa taaaagaaga gcaaaataga tataaaaccg gctaaagaaa atcttataga
     4321 aaaaaattat atgttttgca taaaaatttt aatttttaca aaatttttaa tgattatccg
     4381 caatgattcc tattgtaaat tcttgacttg aaatcggagt tgt
//
LOCUS       DAOTAX010000110         4410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_16218_length_4410_cov_31.827325, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000110 DAOTAX010000000
VERSION     DAOTAX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16218_length_4410_cov_31.827325"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..87
                     /locus_tag="PLM04_08255"
     tRNA            1..87
                     /locus_tag="PLM04_08255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Ser,seq:cga)
     gene            259..3420
                     /locus_tag="PLM04_08260"
     CDS             259..3420
                     /locus_tag="PLM04_08260"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HON03099.1"
                     /translation="MLKQYLNSLQATFNQGDAREESYYGHLKDLLLNYTDERQTNKVD
                     ITVLPKPTEAGNPDFRIWDGKNHITGYIEAKDPSVTNLDRIEVSGQLKRYRETFPNVI
                     LTNFYEFRLYRNGELIKQVLVGRPELARKLKTAPPVENETVFFDLLDTFFSFSLPHVR
                     NAQSLATELAKRTRFLRDEVVSIELDEEEKRGKKAILGFYEAFRKYLIGTLTKEAFAD
                     LYSQTLTYGLFAARTRSDNSFNRELAFKYIPNTIGILRDVFRFISLEDPPKPLQVIVD
                     DIAEVLSVTDVRKILEDYFEEGKGQDPIVHFYETFLTAYDPSIREKRGVYYTPEPVVR
                     YIVRSVNELLKTHFDMPDGLASKNVTLLDPAAGTLTFPAEAVKLAVKEFTEKYGSGGR
                     NNFIRNQVLENFFAFELMMAPYAIGHLKMSFLLEELGYKLNDEERFKLYLTNTLEMDE
                     LEQISIPGLSSLSEESHLAGKIKKEQPVLVILGNPPYSGISANVNEWTEKLLKQNIDG
                     AQSYYEVDGKTLGEKNPKWLQDDYVKFLRFAQWKIQKAGFGVVGMITNHSYLDNPTFR
                     GMRQSLMKTFNEIYILDLHGNSLKKETAPDGGKDENVFDIRQGTAIAIFVKQKEKSDC
                     KVYHAELFGKRQSKYDWLDAHQLEIKNYQLLKPESPWYFFIPRNTQNIKYYLKWKQIN
                     EIFPVNGVGITTARDNFVIDFRKEALLNKIRLFKNSKFSDNELYQFFHINKKIGWNIR
                     KAWNMLQDIPDNELENFIFDFIYRPFDHRYIFWHDSLVWRTVKQIMSHILKENLSITT
                     VRQVKSGETWQHSLVSDKLIESCYISNKTSEICYAFPLYLYNEHTPKKSRTQYMMMLF
                     EPETEYGNSNKEPNIDKKIYQTLNQNYGHELTPEEILYYIYAVFYSNIYREKYAEFLK
                     IDFPRVPFTTDYKVFSKMAALGNELVDLHLMKSSRLNTLVSKFQGEGENDVIEEIIFR
                     PEESRIYINSEKYFDNVAPQVWDYQVGGYRVLQKYLKDRKGRKMDDFVHYSQIITALN
                     YTIEIQQEIDTFYSEAEKEVIEF"
     gene            3607..>4410
                     /locus_tag="PLM04_08265"
     CDS             3607..>4410
                     /locus_tag="PLM04_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HON03100.1"
                     /translation="MKTKITVLLTLMLFCMQAIFSQNQDTLSSHLILFYELNGNGKNS
                     VSDNNHLKLKSQPGNYYYKWTTGKDGKDSTAVVFDGNAQGLKTHLDISPQNLPEITYT
                     AWVFGRPDGFLFGSTLPVNNEKKFSSRSLQISKGAVICGFNKLDSLKNPYFASLRSAT
                     LKKDKWNFIAMTANANDSTLRLYVNDEYYESKPVVFTKKSNFLIIGNTQNSPYNRAFK
                     GQVDEIRVYNKALSSDELSSISGISFADAQKRMERNETIQKVLLAGLIIF"
BASE COUNT         1412 a          761 c          879 g         1358 t
ORIGIN      
        1 ggaaagttgc cggagtggtc gatcgggacg gtctcgaaaa ccgttgtacg ggtaactgta
       61 ccgagagttc gaatctctca ctttccgctt tttttcaaaa gttttttatt ttagtggttc
      121 atgaaaccct aatttttctc ttcatttctt tgtctttaca agcaacacgc tatatttcat
      181 ttcttttaca catgtttttt tccaatctct ttgagtgatt gagtttttta attgtgtttt
      241 tcccatgtta tttattttat gctgaaacag tatttaaaca gccttcaagc gacttttaat
      301 caaggtgatg cacgtgaaga gagttattac gggcatttga aagatttgtt gctgaattat
      361 actgatgaac gtcagacaaa taaggttgac attactgttt tacccaaacc aaccgaagcc
      421 ggcaatcccg attttcgtat ttgggacggg aaaaatcata ttaccggcta tattgaagcc
      481 aaagaccctt ctgttacaaa tcttgacaga atcgaagttt caggccagtt gaaacgttac
      541 cgcgaaactt ttccgaatgt tatccttacc aatttttacg agtttcggct ttatcgaaat
      601 ggagagttga taaaacaagt tctggttgga cgacctgagt tggcaagaaa gctaaaaact
      661 gctcctccgg tagaaaacga aacggttttt ttcgatttgc tcgatacgtt tttttccttt
      721 tcgctgcctc acgtacgcaa tgctcaatcg ctggcaacag agttggcaaa acgcacccga
      781 ttcttgcgtg atgaagtggt aagtatcgaa ctcgatgaag aagaaaaacg tggtaaaaaa
      841 gctattcttg gtttttatga agcttttcgg aaatatctga ttggaacttt gacaaaagaa
      901 gcttttgccg atttgtattc tcaaacattg acgtacggat tgtttgctgc ccgtacgcgt
      961 tctgataatt ccttcaatcg cgagctcgct ttcaaatata ttcccaatac cattggcatt
     1021 ctacgtgacg tttttcgatt tatttcactg gaagatccac ccaaaccttt acaagtcatt
     1081 gtagacgata ttgctgaagt tttgagcgtt acggatgtaa ggaaaattct ggaagattat
     1141 tttgaagaag gcaaggggca agatccaatt gttcattttt acgaaacttt tttgacagct
     1201 tacgatcctt ccatccgtga aaaacgaggt gtttactata cgcctgaacc tgttgttaga
     1261 tatattgtgc ggtcggtgaa cgaacttttg aaaacgcatt tcgatatgcc tgacggactg
     1321 gcttccaaaa atgttacctt gcttgatccg gcagcaggaa cgcttacttt ccctgctgaa
     1381 gccgtaaagt tggctgtaaa agaatttacc gaaaaatatg ggagtggtgg acgaaacaat
     1441 tttatccgta atcaggtttt ggagaacttt tttgcttttg aactaatgat ggcgccttac
     1501 gccattggac atttaaaaat gagttttttg ctggaagagc tgggttacaa actcaatgat
     1561 gaggaaaggt ttaaacttta tttgactaat acgttggaaa tggatgagct ggagcaaatt
     1621 tccatacccg gcctcagttc gttgagtgaa gaaagtcatt tggcaggcaa aattaaaaag
     1681 gaacagcccg ttctggtaat tcttggaaat cctccttaca gcggaatttc ggccaatgtc
     1741 aacgaatgga cggaaaaact gcttaaacaa aatattgatg gagctcaaag ctattatgaa
     1801 gtggacggca aaacgctcgg agagaaaaat ccgaaatggt tacaggacga ttatgtcaaa
     1861 tttttgcgtt tcgctcagtg gaaaattcaa aaagccggtt ttggtgtagt gggtatgatt
     1921 accaaccaca gttatctcga taatccgact tttcgcggaa tgaggcaaag tttgatgaaa
     1981 actttcaatg aaatatacat tctcgacttg catggcaaca gtctgaaaaa agaaacagcg
     2041 ccggatggag gaaaagatga aaatgttttt gatattcgtc agggaacggc aatagccatt
     2101 tttgtaaaac aaaaggagaa atccgactgc aaggtttatc atgccgaact ttttggcaaa
     2161 cggcaaagta aatacgactg gctggatgcc catcaattgg aaattaaaaa ttatcagttg
     2221 ctgaaacctg aaagtccgtg gtatttcttt attccgagaa atacgcaaaa catcaaatat
     2281 tatttgaaat ggaagcaaat aaacgaaatt tttcctgtaa atggtgttgg cataacaact
     2341 gccagagata atttcgttat agatttcaga aaagaagctc ttttgaacaa aataagactt
     2401 tttaaaaact caaaatttag tgataacgaa ctctatcaat tttttcatat taacaaaaaa
     2461 ataggttgga acattcgtaa agcatggaat atgttacaag atattcctga taatgagttg
     2521 gaaaatttta tttttgattt tatttaccga ccatttgatc atcgatatat tttttggcac
     2581 gattcattgg tttggagaac tgtgaagcag attatgtctc atatactgaa agaaaatctt
     2641 tcaataacga cagtaagaca agtaaagtcc ggagaaacat ggcaacatag tttagtttcc
     2701 gataagttaa tcgaaagttg ctatatatca aataaaacaa gcgaaatttg ttatgctttt
     2761 cccctttatc tttacaatga gcatactcca aaaaaatccc gcacgcaata catgatgatg
     2821 ttgtttgaac cggaaaccga atacgggaat agcaacaaag agcccaatat cgacaaaaag
     2881 atttatcaaa cattgaacca aaactacggg catgaactta cgcccgaaga aattctgtat
     2941 tatatttatg ccgtctttta cagcaatatt tatcgtgaaa aatatgccga gtttttgaaa
     3001 atcgattttc cacgtgtgcc tttcacaact gattacaagg ttttcagcaa aatggctgct
     3061 ttgggaaatg aattggtgga tttacatttg atgaaaagca gtcgactcaa tacgcttgtt
     3121 tccaaatttc agggcgaagg cgaaaatgac gttatagaag aaataatttt tcgcccggaa
     3181 gaaagtcgga tttacatcaa ttcggaaaaa tatttcgata atgtagcacc tcaggtttgg
     3241 gactatcagg ttggaggtta tcgggtactt caaaaatatc tgaaagacag aaagggaaga
     3301 aaaatggatg attttgttca ttattcgcaa ataattacgg cattgaatta taccattgaa
     3361 attcaacagg aaatagatac tttttattct gaagctgaaa aagaggtaat cgaattttga
     3421 tattacagct ttttgaaaat tttcttgact gtgagagggc atttcgacag aatttgaaaa
     3481 ttagtgttgt ttttgtaagt cagtatgaaa aatcaattca aataagaaac tgcgtgtaga
     3541 taaattgttt ttttgtttgt ttacaggcaa aatgcaaata ttttttttaa caattaaatt
     3601 taaaccatga aaactaaaat cactgtttta ctgacgctca tgcttttttg catgcaggca
     3661 attttttccc agaatcagga cacgctgtcg tctcatctga ttctttttta cgaattgaat
     3721 ggaaacggca aaaattcggt ttccgataat aatcacttaa aattgaaaag ccaacccggc
     3781 aattactatt acaaatggac gactggcaag gatggtaaag acagcacggc tgtggtattt
     3841 gatggaaatg ctcaaggact taaaacacat ctggatattt cacctcaaaa ccttcccgaa
     3901 attacctata cggcatgggt tttcggacgt ccagatggat ttctttttgg ctcgacactt
     3961 ccggtaaata atgaaaaaaa attcagttcc cgatcgcttc aaatttctaa aggtgccgta
     4021 atatgtggat tcaataaatt ggattctctg aaaaatcctt attttgcatc gcttcgaagt
     4081 gcgacattaa agaaagacaa atggaatttt attgccatga cggccaacgc aaatgattct
     4141 accttacggc tttatgtcaa tgacgaatac tacgaatcga aaccggtagt ttttacaaaa
     4201 aaaagcaatt tcctgattat tggaaacacg caaaattcgc catacaaccg ggcttttaaa
     4261 ggacaggtgg acgaaattcg ggtttacaac aaggcgcttt cttccgatga gttgtcgtcg
     4321 attagtggaa tttcctttgc tgatgcacaa aagagaatgg aacgaaatga aactattcag
     4381 aaagttttgc ttgccggatt gattattttc
//
LOCUS       DAOTAX010000111         4352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_16466_length_4352_cov_28.375844, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000111 DAOTAX010000000
VERSION     DAOTAX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16466_length_4352_cov_28.375844"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..805
                     /locus_tag="PLM04_08270"
     CDS             152..805
                     /locus_tag="PLM04_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HON03101.1"
                     /translation="MSDIKFNDELRVPEPTLRRLPWYLAYAKLMMEKGETYLSSTQIS
                     NNIAVDASIVAKDLSYVQISGKTRVGYETHELIIVLERFLGFTESHKAFMFGVGNLGG
                     ALMHDNGLEQFGLKIVAGFDVKYELAGTSINRIPIHHIEKFPELQRQTGVIIGILTVP
                     VDKAQEVSELMIEGGIRAIWNFTPFRIRVPDNVVVQNTSIYAHLAVMFNRLNAIVNK"
     gene            837..1448
                     /locus_tag="PLM04_08275"
     CDS             837..1448
                     /locus_tag="PLM04_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HON03102.1"
                     /translation="MKIFAIGQNYPEHNKELKNDNPVEPVVFMKPDTALLKNNKPFYI
                     PDFSNELHYETELIIRFNRIGKNIQSKFAHRYFDEIGLGIDFTARDLQRKLKAEGKPW
                     EICKAFDNSAVVGDFLPASDFRDIQDIQFHLDINGITVQKGDSKDMIFSVSELIAYVS
                     RFFTLKIGDILFTGTPAGVGCVHPGDRLEGYIGNRRMFNFLVK"
     gene            1602..>4352
                     /gene="porU"
                     /locus_tag="PLM04_08280"
     CDS             1602..>4352
                     /gene="porU"
                     /locus_tag="PLM04_08280"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HON03103.1"
                     /translation="MAQNTITTSHKLIWKGIEKWYADSSSVQVISFQDAHYPYENRLP
                     YFNYRIPAENYSYKAVLTNTVFEAVNTEEEAILSDKAVYSSEPVLETRYLVDRGSSFF
                     DVQMLPFVFQNGKFLKLISFDLSISKTALPQQAKNAFTHSFASSSVLAQGKFVKVRLA
                     DSGIYKLTYEDLVAMGINPSNVRVFGYGGGVLDQNFTQTKPDDLPEVPIWVEKGSDGI
                     FNAGDYILFYAQGVVRWEYDNGKSMFVHKNNSYSQYGYYFITSDAGIGKKIESKPIEL
                     PANATIYQVDEFTDYQLYEKDIISLTKSGKEFYGETFSTKLSYSFAFYFPNVVKNNTL
                     KFAIDVAASSMSNSSFSINLNGSQSKTLSVTKNSGDNYEIAKSASSVYTFTPDNETLT
                     FNITYNKSTSTSTGYLNYLEVNARRKLVMQGAAMRFQNVDYLGKSSYNNYTLSNVNGN
                     VQIWDITDPQNIYRVATTTLPDNKLSFVASGNELHQYIAIDPTAGSSFPKPEIVGVVA
                     NQNLHALQPSDLVIITHPNFLSQAETLAQAHREKDGLSVVVATTEQVYNEFSSGTPDA
                     TSYRWFVKMLYDRAIETNNTADLPKYLLLFGRGSYDNRGIIPNSGDNLILTYQAENSL
                     VLTSSYVTDDYFGFMDDSEGTQVPADLMDVAVGRFPVSTVQQATDVVNKTINYIDNKG
                     KGIWKNQICFLADDGDGALHMRQADSIAVAVAKKVPAFQINKIYLDAFVQEVNASGQS
                     YPLARSRFHNLLRSGMFLLDYTGHAGYAGWTNENILTTADVKSLSNKNLPVWMAATCD
                     FLQFDVKPVSAGEHVILNPLGGGIGLFSAARPVYASQNFFINKYFVENLFKKNGDYLR
                     VGDAVRAAKNSIGIEINKLSYIYLGDPAVRLNYPDQYNVVTSKINDNVALGKDTLKAL
                     STA"
BASE COUNT         1270 a          794 c          860 g         1428 t
ORIGIN      
        1 gcaaattaaa tttgcaaagt taatcatttt gccaaaataa aagaaagctt cttctttttc
       61 cgtttcatct ttcgctcatt tttataaagt ataagtataa atatttcaat aatttgatta
      121 tttttgcagg tgattactaa tctccagaaa gatgtccgat attaaattca atgatgaatt
      181 aagagttccc gaaccaacat tgagacgact gccatggtat ttggcctatg caaaacttat
      241 gatggaaaag ggtgaaacct atttgtcttc tactcaaata tcgaacaaca ttgcagtaga
      301 tgcttccatt gttgccaagg atttatctta tgttcaaatt tcgggaaaaa caagagtcgg
      361 ttatgaaacc catgaattga ttatcgtttt ggaacgcttt cttggcttta cggaatcgca
      421 caaagctttt atgtttggag tcggaaattt gggaggtgct ttgatgcacg acaacgggct
      481 ggaacagttt gggttaaaaa tagtggcagg ttttgatgta aaatacgaat tggcaggtac
      541 tagtatcaac cgcattccca ttcatcatat tgaaaaattt ccagaactac agcgacaaac
      601 aggtgtaatc attggcattt tgactgtccc tgtggacaag gcacaggaag tgtcggaatt
      661 aatgattgaa ggaggaattc gtgcaatttg gaactttact ccttttagaa taagagtgcc
      721 cgacaatgtg gtggtgcaaa atacttctat ttatgctcat ttggcggtaa tgttcaatcg
      781 cttgaatgcc attgttaaca aataattttt ttatcattca ttagttaaag gaaaaaatga
      841 aaatttttgc tatcgggcaa aactatcccg aacacaataa agaattgaaa aatgataatc
      901 ctgttgaacc tgttgtgttt atgaaacccg acacagcgct gttgaagaac aacaaacctt
      961 tttatattcc cgatttttcc aatgaattgc attacgaaac ggaattgatt atacgtttta
     1021 accgcatagg taaaaatatt cagtctaaat ttgcccatcg ttatttcgat gaaattggtt
     1081 tgggaattga ttttactgcc cgcgatttgc agagaaaact caaagccgaa ggaaaacctt
     1141 gggaaatttg caaggcgttt gacaattcgg ctgttgtagg cgattttctt cctgcttcag
     1201 attttaggga tattcaggat attcaatttc atttggatat caatggaata actgtacaaa
     1261 agggtgattc caaagatatg attttttcgg tcagtgaact tattgcttac gttagtcgtt
     1321 tctttacttt aaaaattggt gatattttat ttaccggaac acctgccggt gttggctgtg
     1381 ttcatcccgg cgatcgtttg gaaggctata tcggcaaccg aagaatgttc aattttcttg
     1441 ttaaataatt gagcaactaa tccctgaata ataacttttt gttttgaacg tttttaaatt
     1501 atctgttttt ttgtattaaa ttttatttct cccttgaggg cgcttttatg aaaattaaat
     1561 ctttattttt ttcttttttc ctttctgtca gtggttggtg tatggctcaa aatactatca
     1621 caacttctca taaactgatt tggaaaggaa ttgaaaaatg gtacgctgat tcatcttccg
     1681 ttcaggtcat ttcgtttcag gatgcgcatt atccttacga aaatcgtttg ccttatttca
     1741 attacagaat tccggccgaa aattattcgt acaaagcggt tttgaccaat actgtttttg
     1801 aagctgtcaa cactgaagag gaagcaattt taagcgataa agctgtttat tcttccgaac
     1861 cggtgctgga gactcgttat cttgtcgata gaggaagcag cttttttgat gttcagatgt
     1921 tgccttttgt ttttcaaaac ggcaaatttc tcaaattaat ctctttcgat ttatcaattt
     1981 ccaaaactgc tcttccacaa caggcaaaaa atgctttcac acacagtttt gcttcttctt
     2041 cggtgctggc acagggaaaa tttgttaaag tcagactggc tgattcagga atttacaaac
     2101 ttacttatga agatttggtt gcgatgggta ttaatccatc caatgtcagg gtatttggtt
     2161 atggtggtgg ggttttggat caaaacttta ctcaaaccaa acctgacgat ttgcccgaag
     2221 ttccaatttg ggtggaaaaa ggaagtgatg gaattttcaa tgccggcgac tatatattgt
     2281 tttatgcgca aggcgttgta agatgggaat atgacaacgg caaatcaatg tttgtccata
     2341 aaaataattc ttattcgcaa tatggttatt attttattac atccgatgcc ggcattggta
     2401 aaaaaatcga atcgaaaccc attgaactcc ctgccaatgc aacgatttat caggttgatg
     2461 aatttacaga ttatcagctc tacgagaagg atatcatcag tcttaccaaa tcgggaaaag
     2521 aattttatgg agaaacattt agcaccaagc tttcttatag ttttgctttc tattttccca
     2581 atgttgtaaa aaataataca ttaaaatttg ctatcgatgt tgcagcttcc tcaatgtcca
     2641 actcttcctt ttctataaat cttaatggat cacaaagcaa aacgctttcg gtaacaaaaa
     2701 attcgggaga taattatgaa atcgcaaaat ctgcttcttc ggtttatact ttcactcccg
     2761 ataacgagac attaactttc aatattactt acaacaagtc aacttcaact tccaccggct
     2821 atttgaatta tctggaggta aatgcccgac gaaaattagt tatgcagggt gctgccatgc
     2881 gttttcaaaa tgttgattat ttaggaaaat cttcttacaa caactatact ttgagtaatg
     2941 tcaacggtaa tgtgcaaatt tgggatatta ccgaccctca aaatatttat cgtgttgcaa
     3001 ccacaacact tcctgacaat aaactttctt ttgtagcttc aggcaacgaa ctccatcagt
     3061 acattgctat tgatcctacg gccggtagtt cctttcccaa gccagaaatt gttggggtgg
     3121 ttgccaacca gaatcttcat gcattgcagc cttctgattt ggttattatt acccatccca
     3181 attttctttc acaggccgaa acattggcgc aggctcatag agagaaagat ggactttcgg
     3241 ttgtagtagc aaccaccgaa caggtttata acgaattttc gtccggtaca ccggatgcta
     3301 cttcctatcg ctggtttgta aaaatgttgt atgatcgggc tatcgaaaca aacaatactg
     3361 ccgatttgcc gaaatatctc ctgctgtttg gtcgtggctc ttacgacaac agaggtatta
     3421 ttcccaactc aggtgataat ttgatattga cctatcaggc ggaaaactcg ttggtactca
     3481 cttcttccta tgttacggat gattatttcg gttttatgga cgacagcgaa ggaacgcaag
     3541 ttcctgctga tttgatggat gtagctgtcg gccgttttcc tgttagtact gtacagcagg
     3601 ctaccgatgt ggtgaataaa acaattaatt atatcgataa caagggaaaa ggaatttgga
     3661 aaaatcaaat ttgttttttg gccgatgatg gcgacggagc tcttcacatg cggcaagctg
     3721 acagcattgc agtagcagtg gcaaaaaaag ttccggcttt tcaaatcaac aaaatttatc
     3781 tcgatgcttt tgtccaggaa gtcaatgcca gcgggcaaag ttatcctttg gcaagaagtc
     3841 gctttcacaa tttgttgcgt tccggtatgt ttttgctgga ttacacaggt catgccggtt
     3901 atgcgggttg gacaaacgaa aacatcctca ctacagccga tgtaaaatct ttgtcgaata
     3961 aaaatttgcc cgtttggatg gctgccactt gcgattttct tcaatttgat gttaagccgg
     4021 tttcggcggg agaacatgtc attttgaatc ctcttggcgg tggaattggc cttttttcgg
     4081 ctgcgcgccc ggtttatgct tcgcaaaact ttttcatcaa caaatatttt gtcgaaaacc
     4141 tttttaagaa aaatggcgat tatctgcgtg tgggtgatgc cgtgagagct gctaaaaact
     4201 ctattgggat agaaatcaat aaactttctt acatttattt gggcgacccg gctgtcagat
     4261 tgaattatcc ggatcagtac aacgtggtta cttccaaaat caatgataac gtagctttgg
     4321 gaaaagatac gctgaaagct ctttctactg ct
//
LOCUS       DAOTAX010000112         4265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_16854_length_4265_cov_27.562945, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000112 DAOTAX010000000
VERSION     DAOTAX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16854_length_4265_cov_27.562945"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..922)
                     /locus_tag="PLM04_08285"
     CDS             complement(89..922)
                     /locus_tag="PLM04_08285"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HON03104.1"
                     /translation="MIERFSEDVDVAINREYFGFTGENLSRREVSKKLRKAACAFCRE
                     TLQFDLAKQMNADGIPETLFSVTMEITDVTTVDPEKIFINYRSLFENEQIATEDGYIQ
                     PIVILEVNGRSMSEPLETVAVKSLVDEVFADRPFIDKAFPVATVVPERTFLEKICLLH
                     EEFSKKEQDKIRVNRMSRHLYDIMRMLDTPIAEKALNDIELYKHIIAHRKVFIGLTEF
                     DYNTLVPQSINIIPPESVISKWEDDYNKMQTMIYGNSFSFNELIDRIKQLNERIKDME
                     W"
     gene            complement(954..1118)
                     /locus_tag="PLM04_08290"
     CDS             complement(954..1118)
                     /locus_tag="PLM04_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03105.1"
                     /translation="MNNLIILSDKDKIEIFTTVATLKNLSKEATEKDWWVTAVLRSLF
                     ALPLRRTDFV"
     gene            complement(1111..1716)
                     /locus_tag="PLM04_08295"
     CDS             complement(1111..1716)
                     /locus_tag="PLM04_08295"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HON03106.1"
                     /translation="MQSTNNQIVHKINKSKRGKIFFLKDFAFLGTNIAIRHSLSRLCQ
                     DGMLIRLSAGVYLYPKISELIGTVYPSIEDIAKAIAKSEKARLIPTGLYALNKLGLST
                     QVPMRVIFLTDGTPRNIKIGGKTSIIFKKTIPKYLALKGKITTLVIFALKEIGKNQVL
                     PEELEKIKSVLKHETKENIIHDSLLAPEWIAEIILKLTEDE"
     gene            complement(1868..3271)
                     /locus_tag="PLM04_08300"
     CDS             complement(1868..3271)
                     /locus_tag="PLM04_08300"
                     /inference="COORDINATES: protein motif:HMM:NF037531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03107.1"
                     /translation="MPSAKKQRISGTLYYDVYKGITLELFGDFNEMLFAPISNDSEYD
                     IILGLTSTSKEITLYKTFVSSRGGLNLVQNQEAGIPTSKYIVNYIFEGVHIDKPEDIK
                     FDKLVSEIIDLDEWVGISGFIPVDFEKAKDFCQNKEYQVQYKLPELIIFPLSNELVGQ
                     FNFVMTPSDIKFYYQKEVSLKQKIQFVLKYSKEKPVEDLLNDLFKFQTFLILALYKKT
                     YPKSILLYNANFQKDYGNENIVTREIKLYIHIPDRNKKQEKQKIFMDMLFCYRNIKDN
                     FPAIIKKWFEKYNELESVFNLLFEQFYNDKRFTENTFLNLAQAAESFHAHTRDNTRMP
                     KEEYKKMKKEILSVVNEKYHQWLKEQFNFGNHLNLHTRLDEIISICSCEIVDKIIGDK
                     EIFIKQVKNSRNYYTHYSSDLKKKALKGSDLFYLTQKLKIVLVCAFLLEIGFDKNILN
                     QMLDDRKHRFFHNLANW"
     gene            complement(3310..>4265)
                     /locus_tag="PLM04_08305"
     CDS             complement(3310..>4265)
                     /locus_tag="PLM04_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03108.1"
                     /translation="PTTSVNFGDIERNSTKWYNGYETYYSVLQFTVSNSTPLNTEIPI
                     KIDMSDANGNTWTDTCKITVSTTGANLSYNSYYVYSENNDDGQINKGETVKLNVSLKN
                     TGSSTAKAVKATFSTTSSYITGFSPTTQVSYGDIASNSVKWYNGYETYYSVLQFTVSG
                     STPANTQIPITISITDESGNTWTSTFNVTVVATDANISYNSFYVYSENNDDGQINKGE
                     TVKLNVSLKNTGSSTAKAVKATFSTTSSYITGFSPTTQVSYGDIASNSVKWYNGYETY
                     YSVLQFTVSSSTPANTNIPINISIVDESNNTWSSSFNVIVQ"
BASE COUNT         1275 a          806 c          665 g         1519 t
ORIGIN      
        1 aaccgggatg aaatgctgaa gcgagggaaa accttcgcaa accactgccg cccttatggc
       61 ttatacactg tgttgtatgc aggtttttct accattccat atcctttatt ctttcattca
      121 gttgttttat cctgtcaatc aattcattga acgaaaacga attaccgtaa atcattgttt
      181 gcattttatt gtaatcatct tcccacttag aaatcacgct ttcaggtgga ataatgttaa
      241 tactttgtgg tacaagcgta ttgtaatcaa actctgtcaa tccgataaat actttgcggt
      301 gggcaataat atgcttgtat aactctatat cattcaacgc cttttcagca atgggcgtgt
      361 cgagcatacg cataatatcg tacaaatgac ggctcattct attaacacga attttatcct
      421 gctctttctt tgaaaattcc tcgtgcaaga ggcaaatttt ttccaaaaat gtgcgttctg
      481 gaacaacggt agcaaccgga aatgccttgt caatgaaagg tctgtctgca aaaacctcat
      541 caaccaacga cttaactgct accgtttcga gcggctcgct cattgagcga ccattcactt
      601 caagaattac aattggctgg atgtaaccgt cttcggtggc aatttgttca ttttcaaaaa
      661 gcgaccggta atttataaaa atcttttcgg ggtcaacggt tgtaacatcg gtaatttcca
      721 ttgttacgga aaacaacgtt tcaggtattc cgtctgcatt catttgtttt gccaaatcaa
      781 actgcaacgt ttcgcggcaa aatgcacacg ctgctttccg cagtttttta ctcacttccc
      841 gccttgataa attttcgccc gtaaaaccaa aatattctct atttattgca acatcaacat
      901 cttccgaaaa ccgctcaatc acattccaac acttgcttaa cgaagtacca cctttaaacg
      961 aaatctgttc ggcgtaatgg caaggcgaac aaagaacgca aaacggctgt aacccaccaa
     1021 tccttttcag tagcttcttt cgacaagttt ttcagtgtgg caactgtcgt aaaaatttcg
     1081 attttatctt tgtctgacaa tatgattaag ttattcatct tctgtaagtt ttaaaattat
     1141 ttcggcaatc cattcgggcg caagtaacga atcgtgaatg atattttcct tggtttcgtg
     1201 cttcaaaacc gattttattt tttccaattc ttctggcaac acctgatttt ttccgatttc
     1261 tttcaaagcg aaaattacca aagttgtgat tttacctttc aatgccaagt attttggaat
     1321 ggtttttttg aaaattatac ttgttttccc gccgattttt atgtttcgcg gcgtaccgtc
     1381 tgtcagaaaa atcacgcgca taggcacttg tgtagaaagt cccaatttgt tgagagcgta
     1441 cagacctgtc ggaataagcc gtgccttttc gcttttggcg attgctttgg caatatcttc
     1501 gattgaagga tagaccgtgc ctatcaattc actgattttc ggataaagat aaacgcctgc
     1561 cgaaagtcga atcagcatac catcttgaca caaccgtgat aaagaatggc ggatagcaat
     1621 attcgttccc aagaaagcaa aatctttcag gaaaaatatt tttccacgtt tgcttttatt
     1681 tatcttatga actatttgat tatttgttga ttgcatacta ttttactttt tatttttggc
     1741 acaaaataaa cctttttttg taccaaagtt cgactgcaaa tgtacaattt ttttttgaaa
     1801 taaacgagcg aacaaaaaaa cagtggaaga ataaatttcc gctgttttgt aaggaataaa
     1861 taacatctta ccaatttgct aaattgtgaa aaaatcgatg ttttctatca tcaagcattt
     1921 gatttaaaat attcttgtca aaacctattt cgagcaaaaa ggcacaaact aaaacaattt
     1981 tcaatttctg tgtcaaataa aataaatcac tgccttttaa tgctttcttc tttaaatcag
     2041 aagaataatg agtataataa ttccttgaat ttttaacttg cttgataaat atttctttgt
     2101 cgccgattat cttatcaact atctcacaag aacagataga aataatctcg tctaaacgag
     2161 tatgcaaatt caaatgattg ccaaaattaa actgttcttt cagccattgg tgatattttt
     2221 cattaacaac agataaaatt tcttttttca tttttttgta ctcttctttt ggcattcttg
     2281 tattatctct tgtatgtgcg tgaaatgatt ccgccgcctg tgccaaattc aaaaatgtat
     2341 tttcggtaaa tcttttatcg ttataaaact gttcaaataa caggttaaaa acagattcca
     2401 actcattgta cttttcaaac cactttttta ttattgcagg aaaattatct tttatatttc
     2461 tgtaacagaa taacatatcc ataaatattt tttgtttttc ctgtttttta tttcggtctg
     2521 gaatatggat ataaagtttt atctctcttg taacgatatt ctcattcccg taatctttct
     2581 gaaaattagc attataaagc aatatacttt tagggtatgt ctttttgtat aatgctaata
     2641 tcagaaaagt ttgaaatttg aataaatcat tcaacaaatc ttcaaccggt ttttctttgc
     2701 tgtattttag aacaaattga attttctgtt ttaatgaaac ttccttttga tagtaaaatt
     2761 ttatatcgga aggtgtcatt acaaaattaa actgtcccac taattcattc gacaacggga
     2821 atataataag ttcgggaagt ttatattgta cctgatattc tttgttttgg caaaaatctt
     2881 ttgccttttc aaaatcgaca ggaataaaac cacttatgcc aacccattca tctagatcaa
     2941 ttatttcaga caccaatttg tcaaatttta tatcttccgg cttatcaata tgaactcctt
     3001 cgaaaatata attcactatg tatttacttg tcggtattcc tgcttcctga ttttgaacta
     3061 aattaagccc acctcttgaa cttacaaaag ttttatacaa agttatttct ttgcttgtgc
     3121 ttgttaatcc taaaataata tcatattcac tatcattgga aattggcgca aatagcattt
     3181 cattaaaatc gccaaacaat tctaatgtaa tgcccttata tacatcataa tataatgttc
     3241 cacttattcg ctgttttttt gcagagggca aaaatcattc gcctttatat tcaaaatttt
     3301 ctgtcatatt tattgcacta ttacattaaa actgcttgac cacgtattat tgctttcatc
     3361 aactatactg atatttattg gtatgtttgt atttgcaggc gtggaactcg aaacggtaaa
     3421 ttgcaaaaca gagtaatatg tttcgtaacc attataccat ttcacagaat ttgaagcaat
     3481 atcgccataa ctcacttgcg ttgtaggcga aaaccctgta atatacgaac ttgttgttga
     3541 gaatgtcgct ttaactgctt ttgccgtact cgaacctgta tttttcagac tgacattcag
     3601 tttaacagtt tcgcctttat tgatttgtcc gtcatcgttg ttttccgaat agacataaaa
     3661 actgttgtac gaaatattcg catcggtggc gactaccgtc acattgaaag tgcttgtcca
     3721 agtgttacca ctttcgtctg taatactgat tgttatcggt atttgagtat ttgcaggcgt
     3781 agaacccgaa acggtaaatt gcaaaacaga gtaatatgtt tcgtaaccat tataccattt
     3841 cacagaattt gaagcaatat cgccataact cacttgcgtt gtaggcgaaa accctgtaat
     3901 atacgaactt gttgttgaga atgtcgcttt aactgctttt gccgtactcg aacctgtatt
     3961 tttcagactg acattcagtt taacagtttc gcctttattg atttgtccgt catcgttgtt
     4021 ttccgaatag acataataac tgttgtacga taaatttgcc cccgttgtag atacagttat
     4081 tttacaagtg tcagtccaag tattaccatt agcatcagac atatctattt taatagggat
     4141 ttccgtattt aacggagttg agtttgagac tgtgaattgc aggactgaat aataggtttc
     4201 gtatccgttg taccattttg tagaatttct ttcaatatct ccaaaattca cagaagttgt
     4261 aggag
//
LOCUS       DAOTAX010000113         4164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_17357_length_4164_cov_34.465563, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000113 DAOTAX010000000
VERSION     DAOTAX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17357_length_4164_cov_34.465563"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PLM04_08310"
     CDS             <1..1144
                     /locus_tag="PLM04_08310"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HON03109.1"
                     /translation="ILLSVSNLQAQPRIWNLNQINSAKNTNSEVVKAVIKEADKHLTT
                     TIATVMDKPLTPASGDKHDYMSMGRYWWPNPDTKDGLPYIRKDGVSNPEIEKLDRTSL
                     SKMARSVMILSLAYHLTNNDEYAQKAVENLKKWFLDKKTRMNPNMEFGQTIPGRNNGK
                     GRGEGVLDSYSFVEMLDAVELLKSSKKFPKEDQTALKDWFSSYLNWMLTSEIGQEEYN
                     AKNNHGTAFDVQVVRYALFTENNEVADKFVKTFPERRIFSQIEPNGAQPLELQRTKAF
                     GYSVYNLTHFMDMAFIAKSMNQDIFHAISNDGRSITKAIEFLLPYLGKSPTEFPYQQI
                     EEWDKVQIDFCWVLKRVDILMGTDNYKNFYSKYLPDNGKDLNFLIY"
     gene            1177..2211
                     /locus_tag="PLM04_08315"
     CDS             1177..2211
                     /locus_tag="PLM04_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03110.1"
                     /translation="MKKLILLLSLCVAIFTANAQIFTEYFQTAPIGGNLEGYNDWHVS
                     PKSSEAYGVSPKIAEGALFYTGYAGSNIGNVAVLDSAVGATSATQRISTKIVKFGSDT
                     LKAVVGEKIYTAFLANISSHSYRSYRDFFTYEGSKTSSMTRGRVFAKVSADGSELFVA
                     VTKNSSTAADYVESTSIPGLTLYPDVNHLFVLVYETIAGDSNDKITLYINPDLTKSEA
                     EQTNKLVAIDTQTDYSATAGLGINLRQRGISAQIGGIRVGKSWDEVLVEGTNAVNTPN
                     NSRAAIWTSGKNILTNTRGTIKIFNLAGAELISAQTDGCLTTNLSKGLYIAQFNGIDG
                     HTAVSKISLN"
     gene            2367..4055
                     /locus_tag="PLM04_08320"
     CDS             2367..4055
                     /locus_tag="PLM04_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HON03111.1"
                     /translation="MSFTIVNARQTTVTSASEINNGSWSAGDTIVMKNGTWTNQSISF
                     RGTGNESQPIVLMAETPGNVILNGTSKLQFSGKYIVVSGLYFKDGNLSGSDVISFRTS
                     SSQLAENCRLTNTAIENYNPLDSTVDSKWVSIYGKNNKVDHCSFVNKTNSGTLMVVWL
                     TSGITVNHEISENYFGYRNANLDANGNELNGQEIIRIGDSSTSMTIAAVTVKNNFFEH
                     CNGEIETISNKSCGNTYYNNLFYECKGMLTLRHGNGCTVKGNYFFGNGISNTGGVRII
                     GENHNVYNNYFENLRGTNYRAALCIVRGKENSALNEYFQVKNATVVFNTFVNCSQAFR
                     INYNSSSSLTMPPIGTVIANNHVYNTSSSYYNVSIDLTNATGMDVTWSNNLMNIGRYT
                     NFTYDENQVITGKDAKMIPVSTPIPMYEPSIGSELVNYVTADYPEIINDIRGRERNST
                     AKLPGASEINGTVSSSMPVKTLVGASFYNNPNTGIKNTNTSFKPFTWLIQQHQIIFNS
                     FETGNLYLVDMSGKVLLNWLVPLNTQLITKTLEKGIYLMVFKTPNKQYTEKIIF"
BASE COUNT         1417 a          719 c          801 g         1227 t
ORIGIN      
        1 gattttgctt tcagtgtcga atttacaggc tcagccgaga atttggaatt tgaatcaaat
       61 aaacagtgct aaaaatacca attcggaagt tgtgaaagca gtcataaagg aagccgacaa
      121 acatctgact acaacgattg ctactgtaat ggataaacca ttaactccgg caagtggaga
      181 caaacatgac tatatgagca tgggacgtta ttggtggccc aatccggaca caaaagacgg
      241 attgccatac attcgtaaag acggcgtttc caatcctgaa atagaaaaac tcgatcgaac
      301 ttcgctttcc aaaatggctc gaagtgtcat gattctttcg cttgcctatc atcttacgaa
      361 taatgatgaa tatgcccaaa aagcagtgga gaatttaaaa aaatggtttc tcgataaaaa
      421 aacaagaatg aatccgaaca tggaatttgg gcaaaccatt ccgggacgca acaatggaaa
      481 ggggcgcgga gaaggtgtct tggacagtta ttcatttgtc gaaatgctcg atgcggttga
      541 attgctgaaa tcttccaaaa agtttccgaa ggaagatcaa acagctttga aagattggtt
      601 ttcgtcgtac ttgaactgga tgttgacttc cgaaatcgga caggaagaat acaatgccaa
      661 aaacaatcat ggaactgctt ttgacgtgca agtagtgaga tatgctttat ttacagaaaa
      721 taacgaagtt gcggacaaat ttgtaaaaac ttttccggaa agaaggattt tctcccaaat
      781 agagcccaat ggtgcacaac ccttagaatt acaacgaaca aaagcatttg gttattcggt
      841 atataatctg acccatttta tggatatggc tttcatagca aaatccatga atcaagatat
      901 ttttcatgct atttctaacg atggaagaag tatcacaaag gcaatagaat ttttgctccc
      961 ttatttagga aaatctccta ctgaatttcc ctatcagcaa attgaagagt gggataaagt
     1021 tcaaatagac ttttgctggg tattgaaaag agtcgatatt ctgatgggaa cagataatta
     1081 taaaaatttt tattctaaat atttgccgga taatggaaaa gacttgaatt ttttgattta
     1141 ttaaataata atgtttcact tttaaattta ttcattatga aaaagttaat tttactactt
     1201 tcgctctgtg ttgccatttt tacggcaaat gcacaaatct ttacggagta ttttcaaact
     1261 gcacctattg gtggtaattt ggaagggtat aatgattggc atgtttctcc taaatcttca
     1321 gaagcttacg gtgtgagtcc caaaattgct gagggggctt tgttttacac cggttatgca
     1381 ggttctaata ttggtaatgt agcagtattg gattctgcag tgggtgcaac aagcgctaca
     1441 caaagaatct ctacaaaaat tgtaaaattt gggagcgata ctttgaaagc tgtagttgga
     1501 gaaaaaatct acacggcatt tttggccaat atttccagcc acagctatcg ctcttatcgc
     1561 gattttttta cttatgaagg ttcaaagact tccagtatga cacgtggaag agtttttgca
     1621 aaagtaagtg cagatggctc agaacttttt gtagctgtta caaagaattc ttctactgct
     1681 gctgattatg tagagtctac ttctattccg ggattaactt tgtatcccga tgttaatcat
     1741 ttatttgttt tagtttatga aacaatcgca ggtgatagca atgacaaaat tacgctgtac
     1801 atcaatccgg atttaacaaa atcggaagcc gaacaaacca acaagttggt tgctatagat
     1861 actcaaacag attatagtgc cactgccggt ttgggaatca atctgcgcca gcgaggaatt
     1921 agtgctcaaa ttggtggaat aagggttgga aaatcttggg atgaggttct tgtggaaggc
     1981 accaatgcag tgaatacacc caataatagt agagctgcca tttggaccag cggtaaaaat
     2041 attctgacca acacaagagg aacaataaaa atattcaatc ttgccggagc agaattaatt
     2101 tcagcccaaa cggatggctg cttgacgacc aacctgtcaa aaggtttata tatcgctcag
     2161 tttaacggta ttgatggaca tacagctgta tcaaaaattt ctttgaattg agtttaattc
     2221 aatgatattt aatttatttt tcattgctta agcattctat cagggaaaac agaaaaaaac
     2281 aatctgtttt ccctacttaa aaaaatttta tgattttaac ttcatcgaac ccaagcggca
     2341 gactattttt actaataact ttctttatga gttttaccat cgtaaatgct cgacaaacga
     2401 cggttacttc agcttcggaa atcaacaatg gcagttggtc ggcgggagac acgattgtta
     2461 tgaaaaacgg cacatggacc aatcaatcca tatctttcag aggtaccggg aatgaatcgc
     2521 aaccaattgt actgatggca gaaactcctg gtaacgtaat tttgaatgga acttcaaaac
     2581 tccaattttc cggtaaatac atagttgtca gcggacttta tttcaaagat ggaaacttga
     2641 gtggttcgga tgttatttca ttcagaacct cttcttctca acttgccgaa aattgcagac
     2701 taacgaatac agccatagaa aattataatc ctttagacag taccgttgac agcaaatggg
     2761 tttctattta tggaaaaaac aataaagtgg atcattgttc atttgtaaat aagaccaatt
     2821 ccggaacttt aatggtagtt tggcttacca gtggaataac agtgaatcat gaaatttcag
     2881 agaattattt tgggtatcga aatgccaatc tcgatgcgaa tggcaacgaa ctgaacggac
     2941 aggaaatcat tcgcataggc gatagctcta cctcaatgac aatcgccgca gtaacagtca
     3001 agaataattt ttttgaacat tgtaatggcg aaattgaaac gatttcgaat aaatcgtgcg
     3061 gaaatacgta ttataacaat cttttttacg aatgcaaagg tatgcttaca ttacgtcatg
     3121 ggaatggatg taccgtgaaa ggaaattact tttttggcaa tggaatttct aataccggag
     3181 gggtaagaat tattggagaa aatcacaacg tttataataa ttattttgaa aatttgcgtg
     3241 gtacaaatta tcgggcagca ctttgcattg ttcgtggaaa agaaaattcg gcactgaacg
     3301 aatattttca agtaaaaaat gcaaccgtag ttttcaatac tttcgtcaac tgctcacagg
     3361 ctttcagaat caattacaac agcagctcat cactgactat gcctccaatc ggaacggtca
     3421 ttgccaacaa ccacgtttat aatacctctt catcgtatta caacgtaagc attgatttga
     3481 caaatgcaac tggaatggat gtaacttggt caaacaattt gatgaatatt ggacgataca
     3541 caaattttac ttatgacgaa aatcaagtaa taacaggtaa agatgcaaaa atgattcctg
     3601 taagtactcc tattcccatg tatgagccat caataggttc ggaattggta aattatgtaa
     3661 cggccgatta cccagaaatc atcaacgata taagaggcag agaaagaaac agcaccgcaa
     3721 aattgccggg tgcaagcgaa ataaatggta cagtttcttc atccatgcca gttaaaactt
     3781 tagtaggtgc ttctttttat aataacccaa acaccggtat aaaaaatacc aatacttcat
     3841 tcaagccatt tacatggctt atccaacaac atcagataat tttcaatagt tttgagactg
     3901 gaaatctata tttggtcgat atgtcgggaa aagtactgtt aaattggttg gttccactta
     3961 acacacaact tattaccaaa accttagaaa aagggattta cttgatggtg tttaaaactc
     4021 cgaacaaaca atacaccgaa aaaattattt tttgaagaag taaaacacaa gaggaattaa
     4081 aatatttaaa aaaatttgga tactgcaatt aaaacagtaa ttttgtcgac actaattggt
     4141 cgaccaatct atttcgttat gaaa
//
LOCUS       DAOTAX010000114         4035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_18027_length_4035_cov_29.933668, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000114 DAOTAX010000000
VERSION     DAOTAX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18027_length_4035_cov_29.933668"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..2945
                     /locus_tag="PLM04_08325"
     CDS             483..2945
                     /locus_tag="PLM04_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HON03112.1"
                     /translation="MKRFFFSFFLSILLHTGYSQFNDSLFSYSCSKNTVVVQNDNSII
                     KVEAWCDNIFRISWFPDMHNIEIDSSHSVILNQAPEVKFSVKEKHNELIIKGRLIVAK
                     ITKIPFNISFYKPDGSILLQPAGAFECDGSSKKASFKIRETDHFYGMGQKSIAVDRRG
                     EAFEVFNQHIGGYTKPYGTMQINIPYIYSTTGFGVYFDNQYPARFDLGKTNPTEWYYE
                     VENGSFTYYLVAAPNEQSLLKSYFDLTGYPPLQPKWIYGLIQSKCSYENDTMVYNTLQ
                     KFEQRKLPVDAIVLDIPWFGGLKPTSPHFMGNFSWNLQNFPDPIKYINTLRGKNVKTI
                     LINEPYINLDSYNYKFLKEKRWLVSKKNQSEPYVFSPFWAGDVSLFDITHPDAQKWLW
                     QQLKAEIEFGADGLWSDLVEPESPVVDGQFYLGDWRKVHNIYSLLWAKNIYDGFSTDF
                     PNKRITNLSRAGFGGIQRYGALCWSGDASKTWFALKLQIPMMTGTVLSGIPYFSSDIG
                     GFTFAHDRRDGNTIFTNIVDGRMKTEPELYIRWFQHGVFSPMLRPHSGEEQFCEVFAF
                     DSLTESITTKYLHLRYRLIPYMYSYSYKTYKKGETLIRPLFFEFSDSLARKQDYQYMF
                     GKEMLVAPVLDEGKTHQQVYLPSENQTWIDFWSNKTYRGGQTIDYNAPLDIIPVFVKA
                     GSIIPLAKNKMRAEEPDDTLTLRIYPDKTAKFEIYEDDNKTKNYQKGEFGITNITSWL
                     CNHQLTINIDAMQGEFENKITERTWIIEIPVSAECKSIKLNNKKLTNFTFDKKNGMLH
                     FEFKADTRVKQKIQIDKFRTTY"
BASE COUNT         1377 a          665 c          703 g         1290 t
ORIGIN      
        1 aagcaataac gtaccaatac gacctgtttg attcaaaggg tcaggatgtt attcgttctt
       61 tcgcagggag tgaagccgta agcggagctc aggcgataaa ggcgttgcaa gtaaaagaag
      121 caggcgaaca ggaacgagcc ttaattcacg atttgatggg aggtttgtaa aatctcacat
      181 taaattacga gaggttaaac agttaaaaga aaccgccgtg tgcgagagca tgcacattga
      241 tgtgagagag gggcgattcc cctacctact tgattataca ttacttcagc ccctcataaa
      301 acttgatttt ttctgccttt agaaaaatta agttttatga ggggattcaa ttttacataa
      361 atatcttcag ggccagaaaa tgtacataaa ttgttagaat agtattattc tctgaaaatt
      421 taaagttata tatttgtaga cccaactgat tcatccgtca gacaagaata aatttaatcc
      481 aaatgaaaag attttttttt agcttttttt tatcaatcct tttacacaca ggttattcac
      541 agttcaacga cagcttgttt tcatactcct gttcaaaaaa tacagtcgta gtccaaaatg
      601 ataactcaat tatcaaagtg gaagcctggt gcgataatat atttcgcatt tcgtggtttc
      661 ccgacatgca taatatagag atagattcat cgcattcagt tattttaaat caggcgcccg
      721 aagtcaaatt ttctgtgaaa gagaaacaca atgaacttat tatcaaaggc agactgattg
      781 ttgcgaaaat taccaaaata ccatttaata ttagctttta caagcctgac gggtctatat
      841 tgctccagcc agctggtgct tttgaatgtg atggtagttc caaaaaggct tcgtttaaaa
      901 tacgggaaac agatcatttt tatggaatgg gacagaagag catcgccgtc gatcgtcgtg
      961 gcgaggcttt cgaagttttt aatcagcata ttggtggcta tactaagcct tatggtacca
     1021 tgcagataaa tattccatat atatattcta caaccggatt tggagtttat tttgacaatc
     1081 agtatccagc cagatttgat ttaggcaaaa ctaatccgac agaatggtat tatgaggtcg
     1141 aaaacggttc tttcacatac tatctggtag ccgcacctaa tgaacaatca ctgctgaaaa
     1201 gttattttga tctcaccgga tatccaccct tgcagcctaa atggatttat ggtttgattc
     1261 agagcaaatg ttcgtacgaa aatgacacaa tggtttataa tacgcttcag aaatttgagc
     1321 agagaaaact gcctgtcgat gctattgtac ttgatatacc atggtttggc gggctcaaac
     1381 caacttcgcc acattttatg ggtaattttt cgtggaacct acaaaatttt cccgatccta
     1441 tcaaatatat aaatacatta cgtggaaaaa atgtcaagac gattttaata aatgagccat
     1501 atatcaacct cgattcatat aattataaat ttctcaagga aaaaagatgg ctcgtttcaa
     1561 aaaaaaatca atccgaacct tatgttttta gtccgttttg ggcaggcgat gtttcacttt
     1621 ttgatataac tcaccccgat gcccaaaaat ggctttggca acaactgaaa gcggaaattg
     1681 aattcggtgc cgatggactt tggagcgacc tcgttgaacc cgaaagtcct gttgttgacg
     1741 gacaatttta tttgggcgac tggagaaaag tacataacat ctatagttta ttgtgggcaa
     1801 aaaacattta tgatggtttt tcaactgatt ttccaaacaa aagaatcaca aatttaagca
     1861 gggcagggtt tggcggcatt caacgttatg gagcattgtg ctggagtggc gatgcatcta
     1921 aaacctggtt tgctcttaag ttacaaatac ccatgatgac aggtactgtg ctttcaggga
     1981 taccttattt ttccagcgat ataggtggat ttacctttgc ccacgacagg cgtgatggca
     2041 ataccatttt tacaaatatt gttgatggac gaatgaaaac cgaacctgaa ttgtacatac
     2101 gttggtttca acatggtgta ttttcgccaa tgttacgtcc acacagtggt gaagaacagt
     2161 tttgtgaagt ttttgcattc gacagtctga ccgaaagtat aacaacaaaa tatttacacc
     2221 tgagatatcg gttaatacct tatatgtatt cgtattcata caagacctac aaaaaaggcg
     2281 aaactctaat ccgtccgtta ttttttgaat tttcagactc cttagctcgt aaacaggatt
     2341 atcaatatat gtttggcaaa gaaatgcttg tagctcctgt acttgacgaa ggtaaaaccc
     2401 atcaacaggt ttacttgcct tcagaaaacc aaacatggat tgatttctgg agtaataaaa
     2461 cttatcgggg aggacaaact atcgattata acgctccact tgacattatt ccggtttttg
     2521 ttaaagccgg ttctataatt ccattagcta aaaataaaat gcgcgctgaa gaacccgatg
     2581 acacacttac tttacgtatt tatcctgata aaacagctaa gtttgaaata tacgaagatg
     2641 acaacaaaac caaaaattat caaaaaggag aatttggaat tacaaacata accagttggt
     2701 tgtgcaatca tcaacttaca attaatattg acgcaatgca gggagaattt gaaaataaga
     2761 ttacagaacg tacatggatt atagaaattc ccgtctccgc tgaatgtaaa agtataaaac
     2821 tcaacaataa gaaattaaca aatttcactt ttgataaaaa aaatggaatg ttgcatttcg
     2881 aattcaaggc cgatacaaga gtaaaacaaa aaattcagat tgataaattt agaacaacct
     2941 attaaatttc acattagaaa tccgtttcga ttttactgta tggtttaaag aatctaatca
     3001 gaaagaatcc aaatcttagc agcttttata aacaaatggg ttttgcttag atttgcgtat
     3061 caaattctcc aacaaccatt tctgagtaac ttcagagaaa cctactacaa caacgaaaaa
     3121 ccctttgaac atctattgga tattcataaa agaagttttt tgttaaaacg tcgatcctta
     3181 aagaccccat ttttgtctta aaatattgtt ggcttggtaa aacatagggt ttatcaagtc
     3241 aacaatttgt attttaaatt cgagccaagt agataattcc catttattca tcattctttt
     3301 gaagatacat gcagctgcag caagcattac attttatcgc ctgtaattcc tttgtaaaaa
     3361 ttacgattta agcaatggtc tgtttttaaa tgacctcaca aaaaagtaag tgaggtttgg
     3421 tgaaagcccc gttattaaat atctgaaaat aaaaaatata aacgttttta caattttttt
     3481 gttatgtcct aaggaaaaaa acataaagaa gtgttataaa atttattttc taacgttttc
     3541 gagccaactg ttagatatac tttcaatttt attgattaaa aattaacata aaatgtagtg
     3601 atttttgtat caactgtcat ttttttgctg agtaaaatgt taaatatttg attttaacaa
     3661 aaaatttttt gttgaatgaa atttgtttat ttatggcaat atttttttaa cagaaaattt
     3721 taagaaatca gtaaaacatt tattcccaga catttgtaaa gatattttta tttgtaaaat
     3781 tatcgatggt acttaaaaag ggatacgaat tttggcaata aactgcaaaa tgaaagggaa
     3841 aaattgaata ttttttctat ttgtcatcaa aaacacttgt tttttatttt ttaatatttt
     3901 atttttgcca gcacgttttc cctgctatag gtgaaaataa taaataataa gattaataaa
     3961 caaaaatgta aaatttatga gaaaactaac attcttattt gcctgcctat ttatgatagg
     4021 agttggtttg gtcaa
//
LOCUS       DAOTAX010000115         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_18671_length_3920_cov_33.243984, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000115 DAOTAX010000000
VERSION     DAOTAX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18671_length_3920_cov_33.243984"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..399)
                     /locus_tag="PLM04_08330"
     CDS             complement(19..399)
                     /locus_tag="PLM04_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HON03113.1"
                     /translation="MNNKSGYPVKKFSQPVKRYCQTLKLKDDATLIEEYKFWHAAENR
                     WPEISEGIKAVGILDMELYIHGNLLFMIVETPLDFDWDKAFGKLANLPRQAEWEAFMA
                     KFQMADPNAGSAEKWTLMERIFSL"
     gene            complement(855..2876)
                     /locus_tag="PLM04_08335"
     CDS             complement(855..2876)
                     /locus_tag="PLM04_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON03114.1"
                     /translation="MLFAISMVLSLSAAEKSAAVGGSLQIKNCIVLNDKAYLELSSTV
                     TASNSFIQGTPPSGNSTISSIETLKDVVAPADTVSFYLSPLATTVNAGSNSAAGSLPD
                     KDINGNPRIYDTTVDVGAVEYSLIYEKGINNWNTASHWNIGRVSTENDMVTIRSRCKV
                     TTANAVCKKIISIGDTAYLSIEPNQFLTVKETIQNTNPSCLIVRASTDGSSSNGSLVF
                     HNAESNQVYGTVEMYSKAWIDPNGVTNNKYFWQYFGLPLRSLQANPSFYGAYVRRWDE
                     TGTTISNHWVQLGNNDVIQSFLGYEICQSSPTTYALQGILENRDFSSGELAYTTTALY
                     PGQHIFGNSYTAAIDITQINFGSNMEATVYLYNTGSYNNWVTNNGETSHSPSNTNPGT
                     YAAIPKNQAGQGGIQGQIPSMQGFLVKAMSGSSGNTISIPYSSVAVKNTTLQRAKAAE
                     ENDSEKKQYLRIDLKTDTVIVDRMWIFNEENCTRSFDNGWDGPKMLPASGTPVLCAIE
                     KDGLYQVNSVNDMDETKLGFRKGTETEYSLTFYPKNLQQRYPAVYLLDEAVNQAVKIE
                     NETVYSFTATGTENLQQRFRIVTRYYEKGEPEEASQIKVFSSKGHIVIDNLSAENGEA
                     MIYDMAGRKVGQEKFFGNNITVLNNYPTGVYVVRAFSSKETVTKRIIVQ"
     gene            complement(2910..>3920)
                     /locus_tag="PLM04_08340"
     CDS             complement(2910..>3920)
                     /locus_tag="PLM04_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03115.1"
                     /translation="TLFSLVFRLTGQGQPQELGIYVLFYRYIKQMSQFLSCTKMKRIN
                     LLLVSISLLPFFSFSQNGEGGGAYLQNNGKLYNSIVTHNSSVQGFGVSGTSGEIANSN
                     IVYNTCLPYDVMNIGDLLFDDGTLYRLSYDTNGNIVFPTGYSASRVVGVCCWRNKLNR
                     YENSRNWFIAVDEAQTYWCPNGYVNGGGWNPPDIPNLYNFSDPAAALVDESGKSNTQT
                     IVNCSCFIFRPNGESDTLNTSNCAAKYCTEYKKGGFQWYLPSLGQMYQLEKNLTVINN
                     VLNKLGKIQISNSLYWTSTEISSQQAWQYSFPSSSMRPQYGTKKSNFKVRPMTDLPLP
                     QQ"
BASE COUNT         1141 a          819 c          713 g         1247 t
ORIGIN      
        1 taaatggtaa atggtaattc acaagctaaa aatccgttcc atcaaagtcc atttttctgc
       61 tgaacctgca ttcgggtctg ccatctgaaa ttttgccata aatgcctccc attctgcttg
      121 ccgtggtaag ttggcaagtt ttccgaaagc tttatcccaa tcaaaatcta gaggagtctc
      181 cacaatcata aaaagcaaat tgccatgtat atataattcc atatcgagga tcccaaccgc
      241 ttttattcct tccgatattt caggccagcg attttcagcc gcatgccaga atttatattc
      301 ttcaattaaa gttgcatcat cctttaattt taaagtctga caataccgtt ttaccggctg
      361 tgaaaatttt ttcaccggat atcctgattt attattcata atttgttcat tattaagaaa
      421 atataaaatt aaaaatctgg ttgttattta cgatattcaa ttttcacttt ttctctcctt
      481 ctactcttga tatgaaaccc acatgtattg catacacaaa cacgaatgaa tataatattt
      541 agacatgtgg gttccatcag acctttaaaa aataaattat attctgatga aaactaagta
      601 aagaaaattc atctgtgctg aactgtgctg caaaaatagt caaaaatatg agacgatatt
      661 actttatcat aaaaaattag atttaatcgt taatcgtttg aaacaagtat aaattagcta
      721 cttttgctat aatacaattt tgaatcccct ctcaacccac attgcagaaa gaaaaggcag
      781 aaaatcattc ccagccctcc aaccactaat atcaacgaga gttttatttc ttcctttttc
      841 attgctctcc gcttttactg aacaatgatt cgttttgtta ccgtttcctt gcttgaaaaa
      901 gctcttacca cataaactcc cgtggggtaa ttgttgagaa cggttatgtt atttccaaag
      961 aatttctcct gacccacttt tcgtcccgcc atatcgtaaa tcattgcttc tccgttctct
     1021 gcactcaagt tgtcaatcac aatatgccct ttggaagaaa ataccttgat ttgcgatgcc
     1081 tcttccggtt ctcctttttc gtaataacgc gttacaatac gaaaacgctg ttgcaaattt
     1141 tccgttcctg tagccgtgaa tgaataaacc gtttcatttt ctatttttac cgcttggttt
     1201 actgcctcgt ccagcagata aacggccgga tagcgctgct gtagattttt cggataaaat
     1261 gttaaggagt attctgtttc ggttcctttc cggaatccga gtttggtttc gtccatatcg
     1321 tttaccgagt tcacttgata caaaccgtcc ttttctatgg cacacaatac cggtgttcca
     1381 cttgccggaa gcatttttgg cccgtcccag ccattgtcga aagaacgggt acagttttct
     1441 tcgttgaaaa tccacatgcg gtctacaatt acggtatccg tttttaagtc tatgcgcaga
     1501 tattgttttt tctccgaatc gttttcttca gcagcctttg cacgttgaag ggtggtgttt
     1561 ttcacggcta cggaagaata gggaatggaa atggtatttc ctgacgaacc gctcatggct
     1621 tttaccagaa atccctgcat ggagggaatt tgtccctgaa tgcctccctg accggcttga
     1681 ttcttgggaa tggcggcata tgttcccggg ttggtgttgc tcggagaatg actcgtttca
     1741 ccattattgg ttacccagtt gttgtaagaa cccgtgttgt acagatagac agttgcttcc
     1801 atattgctgc caaaattaat ttgggtgata tcgatggctg ccgtatagga atttccaaaa
     1861 atgtgctgac cggggtacaa ggccgtagtg gtgtaagcca attcgcctga actgaaatcc
     1921 ctgttttcca aaattccctg caaggcgtag gtggtagggg aagactgaca aatttcataa
     1981 cccagaaacg actgaatcac atcgttgttg cccaattgta cccaatgatt ggaaatcgta
     2041 gttcccgttt cgtcccagcg tctgacataa gcgccataga aagaaggatt ggcttgcaac
     2101 gaacgcaaag gcaaaccgaa atattgccag aaatacttgt tattggttac tccattggga
     2161 tcaatccatg ctttggaata catttctact gtgccataaa cctgattact ttctgcattg
     2221 tggaatacga gcgagccatt ggatgaactc ccatcagtgg aagctcttac aatcaaacag
     2281 gatggatttg tattttgtat agtttccttg acagtcaaaa actgattagg ctcgatagac
     2341 aggtaggcag tatcgccgat actgataatt tttttacaaa cagcattagc agtagtaact
     2401 ttgcagcggg aacgaatggt aaccatatca ttttcggtag aaactcttcc aatgttccag
     2461 tgtgaagccg tgttccaatt gttgatacct ttttcgtaaa tcaaggaata ttcaacggct
     2521 cccacatcaa cggtagtatc ataaatacgg ggatttccgt ttatgtcttt atcaggtaac
     2581 gaaccggctg cgctgttaga accggcattt actgtcgtgg caagcggtga caggtaaaaa
     2641 gaaacggtat ccgcaggagc tactacatct tttaatgttt caatgctgct aatggtactg
     2701 ttccctgaag gaggcgttcc ctgaataaaa gaattactgg cagtaaccgt gctggaaagt
     2761 tccaaataag ctttatcatt taaaacaatg caatttttga tctgcaaaga accacccaca
     2821 gcagccgact tttcagcagc agatagtgac aataccatac taatagcaaa taacattcct
     2881 atgtttttga aaaataccat attcttgctt tattgttgag gcaatggtaa gtccgtcatc
     2941 gggcgaactt tgaaattact tttttttgtt ccatattgtg gacgcatact ggaagaagga
     3001 aaactgtact gccatgcctg ttgagaactt atttctgttg atgtccagta taaactattg
     3061 cttatctgta tttttcccag tttattaagc acattattaa ttacagttag atttttctcc
     3121 aattgataca tttgccctaa ggaaggcaaa taccattgaa atccaccttt tttatattct
     3181 gtacaatatt tggcggcaca attggaagta tttaatgtgt ccgattctcc atttgggcga
     3241 aaaatgaagc atgaacaatt tactatggtt tgtgtgttag attttccact ttcatctacc
     3301 aaagctgctg cgggatcact aaagttatat aaatttggta tatcgggagg attccatcca
     3361 cctccattta cgtaaccatt aggacaccag tatgtttgtg cttcatcaac agcaataaac
     3421 caattcctgc tattttcgta acggttaagc ttatttctcc agcagcaaac gcctactacc
     3481 cgtgaagcac tgtaacccgt aggaaacact atatttccat ttgtgtcata cgacaatcga
     3541 tataaagtac catcatcgaa caataaatct ccaatattca ttacatcata aggtaagcat
     3601 gtattataaa caatattact gtttgcaatt tctccactgg ttccagaaac cccaaatcct
     3661 tgcaccgatg aattatgagt aacaatactg ttataaagtt ttccgttatt ttgcaaataa
     3721 gcgccaccac cttcaccatt ctgggaaaag ctgaaaaacg gcaacaaact gatacttacc
     3781 aaaagtagat ttatcctttt cattttagta catgacaaaa attgagacat ttgttttata
     3841 tatctgtaaa acaagacata tatgccaagc tcttggggtt gaccttgtcc ggtgagccga
     3901 aaaacaagtg agaaaagcgt
//
LOCUS       DAOTAX010000116         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_19683_length_3757_cov_27.900864, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000116 DAOTAX010000000
VERSION     DAOTAX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19683_length_3757_cov_27.900864"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLM04_08345"
     CDS             complement(<1..1019)
                     /locus_tag="PLM04_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HON03116.1"
                     /translation="MIEKQKYCTLKRKSRKALLSLLLLFIVEITVWGGTQQNFTVPST
                     MKAEAWPAHVELTFENYQCFDYEIFRSKDGGKNFIKCGVTSTGQYMDFFGKPVSKEET
                     FIYRILPKGMDVKNKNAYKFEQKVTVKHSDDEQLLNMVQHYTTRYFYEFAEPVTGMAR
                     ERSNDRNGEIVTTGGTGFGIMALIAGAERNYFSRQDACRSITKIVSFLEKAERFHGAW
                     AHWYDARTGKPFSFSKYDDGGDLVETAFLVQGLLAAREYFNTENVEEHLLSNRISQLW
                     ETVEWNWYTQGTDSLYWHWSKNYGWKMNHRIKGFDETLITYVLAASSPTYPIEKKVYD
                     ACFKNS"
     gene            complement(1033..3261)
                     /gene="bglX"
                     /locus_tag="PLM04_08350"
     CDS             complement(1033..3261)
                     /gene="bglX"
                     /locus_tag="PLM04_08350"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HON03117.1"
                     /translation="MKNYLSSLVIISFLLMGCTTATQTSTADKKIDAKVDSVLKLMTL
                     DEKIGQMVLYTSDWDVTGPTLKSDYIDDIRSGKCGNIFNAHTVEYVRKLQKIAVEESR
                     LGIPLLFGYDVIHGHKTIFPISLGESASWDLQAIEKSARIAATEAAASGLNWTFAPMV
                     DISVEPRWGRVSEGAGEDPFLGSQIAAARVRGFQGNDLADTTTVLACVKHFAAYGAPL
                     AGRDYNTVDMSQRVFLDVYLPPYKAAIDAGALSVMSSFNEFDGVPATGNKYLLTDILR
                     TQLGFKGFVVSDYTSVNEMVHHGIVANEEEAALLAIKAGLDMDMQGEVYAKYLKGLIE
                     KGLVDEKTIDQSVRRILKVKFKLGLFDDPYRYCNEEREKRTIYAPEHLEAAFDVAKRS
                     MVLLKNENNVLPLKKGEKIAVIGELAASKRDLLGSWKAAGDWNFMKSVLDELKEYNGE
                     KNVLYAEGCKKMGDDRTGFAQAIATARRADKIVMVIGEDAEWTGEAASRSNINVPGVQ
                     TELLEQLKLLNKPLVVVLMNGRPLVLTKENKLADAILEAWFPGTMGGKAITYVLYGEY
                     NPSGKLTMTFPQNLGQVPIFYYEKNTGRPIYLSNDKYKSKYLDCPNDPLFPFGFGLSY
                     TTFEYSDITLSTKELQQNGSIKATVTVTNTGKTDGEEVVQCYIRDMVGSVTRPVKELK
                     GFEKIFLKAGESKEVSFTITPDLLAFHRLDMTYGTEPGDFKLFIGGNSRDVKETSFKL
                     ID"
BASE COUNT         1100 a          797 c          672 g         1188 t
ORIGIN      
        1 gagtttttaa aacaggcatc atacactttt ttttcgattg gataagtggg tgaggaagct
       61 gccagtacat aagttatcag cgtttcgtca aagcctttga tgcgatgatt cattttccaa
      121 ccgtagtttt tcgaccagtg ccagtaaagc gaatcggtac cttgtgtata ccaattccat
      181 tctactgttt cccaaagttg tgagatgcga ttcgacaaca agtgttcttc aacattttcg
      241 gtgttgaaat attcccgtgc agccagcaga ccttgtacca gaaatgcggt ttctaccaaa
      301 tcgcccccat catcatattt tgaaaagctg aagggttttc ccgttcgtgc gtcataccaa
      361 tgcgcccatg ctccgtgaaa tctctcagct ttttccaaaa aggaaacaat ttttgtgatg
      421 gaacgacagg catcctgccg actgaaataa tttcgttctg caccggcaat caatgccata
      481 ataccaaaac cagttccgcc ggtagttaca atttctccat ttcggtcatt gcttctttcg
      541 cgcgccatgc cggtaacagg ttcggcaaat tcataaaaat atcttgttgt gtagtgttgt
      601 accatgttca gtaattgttc gtcatcacta tgctttacgg ttactttctg ctcaaatttg
      661 tatgcgtttt tattttttac atccattcct tttggcaaaa ttctgtaaat aaaagtttct
      721 tcctttgaaa ctggtttgcc aaaaaaatcc atgtattggc cagtagaagt tactccacat
      781 ttgataaaat tttttcctcc atctttcgac ctgaaaattt cgtaatcgaa acactgataa
      841 ttctcaaagg ttaattcaac gtgtgcgggc cacgcttctg ctttcattgt agaaggtact
      901 gtaaaatttt gttgagtgcc accccacact gttatttcaa cgataaataa taacaataat
      961 gaaagtagcg cttttctact tttccttttc aacgtgcaat atttctgctt ttctatcatg
     1021 aaaaaagatt ttctaatcaa ttagtttgaa tgaagtttct tttacgtctc tcgaatttcc
     1081 accaataaac agtttaaaat ctcctggttc ggttccgtaa gtcatatcca gtctgtgaaa
     1141 agccagcaaa tcgggcgtaa tggtaaaaga aacctcttta ctttcgccgg ctttcagaaa
     1201 aattttctca aaacctttca actcttttac tgggcgagtt acactaccca ccatatcgcg
     1261 aatatagcat tgcactactt cttcgccgtc tgttttgccg gtattggtta cggtaacagt
     1321 tgccttgata cttccgtttt gttgcaattc tttggttgac aaagtaatgt ctgaatattc
     1381 aaaagtggta tagctcaatc cgaaaccaaa aggaaacagt ggatcattag ggcaatcgag
     1441 atattttgat ttgtatttat cgttactcaa atatatggga cgtccggtat ttttttcgta
     1501 ataaaatatg ggaacttgac ctaaattttg tggaaaggtc atggtcagtt tgccggaagg
     1561 attatattct ccgtaaagaa cataagttat ggcttttcca cccatcgttc cgggaaacca
     1621 agcttccaga atagcatctg ccaatttgtt ttcttttgtg agaacaagag gacgaccgtt
     1681 catcaatact acaacgagag gtttatttaa caatttcagt tgctcgagta attctgtttg
     1741 cacacccggc acattgatat tcgatcggct ggcagcttct ccggtccatt cggcatcttc
     1801 gccaatcacc atcacaattt tatcggctct gcgggctgta gctattgctt gagcaaatcc
     1861 ggtacggtcg tcccccattt ttttgcagcc ttctgcgtag agtacatttt tttctccatt
     1921 gtattctttc aattcatcca gaacagattt catgaaattc caatctccgg cagctttcca
     1981 cgaaccaagc aaatcgcgtt ttgacgccgc caattcgccg attactgcaa ttttttctcc
     2041 ttttttaaga ggaagaacgt tgttttcgtt ttttagcaaa accattgaac gtttagctac
     2101 gtcaaaagca gcttccagat gttcgggggc ataaatcgtt cgtttttcac gttcttcgtt
     2161 acaataacga taagggtcat caaaaagacc caatttgaat ttcaccttta aaattcgacg
     2221 tactgactga tcaattgttt tttcatccac caagcctttt tcgatcagtc cttttaaata
     2281 tttggcatat acttctccct gcatatccat atcgagacct gccttgatgg ccagcaacgc
     2341 agcttcttcc tcattggcaa caatcccgtg atgaaccatt tcgtttaccg aagtataatc
     2401 gcttacaaca aagcctttga atccgagttg ggttctcaat atatcggtaa gcaaatattt
     2461 gtttcccgta gcaggaacac cgtcaaattc gttgaaagaa ctcataacac tcagagcgcc
     2521 agcatcaatg gcagctttgt aaggaggcag ataaacatcg agaaatacac gttgcgacat
     2581 gtccactgtg ttataatcgc gtcccgccaa cggtgctcca taagcagcaa aatgttttac
     2641 acaggctaaa actgttgtcg tatcggctaa atcatttccc tgaaaacctc tgacacgggc
     2701 cgcagcgatt tgagaaccca gaaaaggatc ttcgcccgcc ccctcagaaa ctcttcccca
     2761 gcgcggctct acagaaatgt caaccatggg cgcaaaagtc cagtttaatc cggatgcagc
     2821 agcttcggtg gccgcaatac gtgctgattt ttcaatagct tgcaaatccc acgaagccga
     2881 ttcccccaac gaaataggaa aaatagtttt gtgcccatga atgacatcgt aaccaaacaa
     2941 caacggaata cctaaccgtg attcttctac ggcaattttt tgaagtttgc gtacatattc
     3001 aaccgtatgt gcgttgaaga tgttgccgca tttgccactc cgtatatcgt ctatgtagtc
     3061 gctttttaat gttggaccgg taacatccca atcactggta taaagcacca tttgtccgat
     3121 tttctcatcc aaagtcatca attttaatac agaatcaact ttggcatcga tttttttgtc
     3181 agctgtggaa gtttgagttg cagtagtaca acccataagc aggaaagaaa taattaccaa
     3241 tgatgaaagg tagtttttca tggttcaacg ttttaaaatt tcgatttcaa ataacgttgc
     3301 aaagaaggag ataattaaaa gcgtttgcaa atttttatac cctataacaa ccccactggt
     3361 gaaaatattt ttaatttata cccctatttt tcccctataa tgagaaaata ttattattta
     3421 aacctttaaa aaacaaataa atatatttta ttttaatttt aaaaaatatt ttgtagaatt
     3481 gcttgaatta aggcaaacta taacctaaaa atttgttaaa ttatggaaag ggagaagaat
     3541 aaatgagtga aacaatatct gttgagtgta atttgacaat ttctaaactg atattagctt
     3601 ataaaaaaaa ccgagcagta aaaaataata gttattttta caactgcgat ggaaaagaga
     3661 aaaaatttga catcaaaaat caaggctgaa attgttttat ccttgttgcc acagggaaaa
     3721 gtcgaagctg caagacgcgt aaaagcaatg gttgcaa
//
LOCUS       DAOTAX010000117         3708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_20001_length_3708_cov_30.694224, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000117 DAOTAX010000000
VERSION     DAOTAX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20001_length_3708_cov_30.694224"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLM04_08355"
     CDS             <1..371
                     /locus_tag="PLM04_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HON03118.1"
                     /translation="NFAFDHNEVILHSAPEGKHQLLLQKDNRVCITFCSDRKLMYQHP
                     DVACSYSMNSKSVLVKGKVIFIENLEEKEQAMNLFMKKYTQRSDFKYSLPALRNVKVW
                     KVKVESITAKAFGQNFKTQQ"
     gene            805..3240
                     /gene="lon"
                     /locus_tag="PLM04_08360"
     CDS             805..3240
                     /gene="lon"
                     /locus_tag="PLM04_08360"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HON03119.1"
                     /translation="MKHSFDDFNIEELTQPSEAFPIISFEEEKNGDVSLESGDILPIL
                     PLRNMVLYPGVLLPVSVARPKSLKLVREAKESNHYIGVCTQKDKNIDDPTPDQLYSMG
                     TVAKVVRILEMNDNTTTVILEGKSRFFLGEVLTLKPYMKARINLAPEIIPSAEDGVFP
                     ALVATIKDMAAKIIANAGIMPPEAVFAIRNIDNPVFLINYVCVNFGLNVQEKQRLLEI
                     DDILDRGYQLLDLLNKEWQLLEIKISIENKAKEGIDQQQKQYFLQQQMKTIQEELGGA
                     SPEQDILDFREKAKQKKWNENVAALFEKELKKLERMSPHSPDYTTQLNYVETLLELPW
                     NEYTKDNFNLKNARKILEQDHFGMETVKERILEHLAVLKLKGDMKSPIICLYGPPGTG
                     KTSLGKSIARALNRKYVRISLGGLHDEAEIRGHRRTYIGALPGRIMQNIQKAQSANPV
                     FVLDEIDKVAADYKGDPSSALLEVLDPEQNTSFHDNYLDVDFDLSKVMFIATANNLNT
                     IPRPLLDRMELIEVSGYTQEEKVEIARRHLVPKELENHGLTKEQLILTKPVIEHLIEN
                     YTRESGVRELDRQIASLMRKIAKKVASNEPYNVQLTVDDLKTFLGVPRYTKEKYQGND
                     YAGVVTGLAWTQVGGEILYIESSLSKSKSPQLTLTGNLGEVMKESAVIALEYIKAHAE
                     QLGIDSEMFEKWNVHIHVPEGAIPKDGPSAGITMLTALVSAFTKRKVRKNLAMTGEIT
                     LRGKVLPVGGIKEKILAAKRAGITELVLCEENKKDVEEINASYLKGLHFHYVNDIMEV
                     INFALLDEKVRME"
BASE COUNT         1233 a          615 c          752 g         1108 t
ORIGIN      
        1 tgaattttgc ttttgatcat aacgaagtta ttttacattc tgcacctgaa ggaaaacatc
       61 agctattact ccaaaaggat aatcgtgtct gtattacgtt ttgttcggac aggaaattaa
      121 tgtaccaaca tcctgatgtg gcatgcagtt acagcatgaa ctccaaaagt gtgctggtga
      181 aagggaaggt gatttttatc gaaaatttgg aagaaaaaga acaggccatg aatttgttta
      241 tgaaaaaata tacacaacgc agcgatttta aatactcgct tccggcactt agaaacgtga
      301 aagtatggaa agtaaaggtg gaatcgatta cagctaaggc ttttggtcaa aactttaaaa
      361 cccaacaata agtaattttt taacctctta atatatttaa ttatttgatt tcctgaacta
      421 tatatttaat ttccttttcg tattttttca atcatttgtt ttctttttct cccacacgaa
      481 aaaaaatttt ataataagaa aacccacatg tagtgcatac acacgaatga atataatatt
      541 cagacatgtg tgtcccatca gacctttaaa aaaataaatt atattctgat gaaagattta
      601 aatgggtatt ttgtgaaaaa ccatttttag aactgccggt ttttaataca gattttttgg
      661 cacaacaatt gaaaaaaata tcgtcgaccc attttctgaa gattttttag aaaagaagga
      721 aacccatttt gttgaatttg aataaattta tgtcattctg acattaaaga tactaatttt
      781 gtcattaaaa ttttaaatag cgcaatgaaa cattcatttg atgattttaa tattgaagaa
      841 ttaacacaac cttcggaagc ttttcccatt atttcgtttg aagaggaaaa aaatggagat
      901 gtttctctcg agtcgggcga tatattgcct attctaccct tgagaaacat ggttttgtat
      961 ccaggtgttt tattgccggt atcggttgct cgaccgaagt cgctgaaact tgtgcgggaa
     1021 gcaaaagaaa gcaatcatta tataggcgtt tgcacacaaa aagataaaaa tattgatgat
     1081 ccaactcccg accagttgta ttctatggga actgtggcca aagtagtacg gattttggaa
     1141 atgaacgata acactactac ggttattctt gaaggaaaat cacgtttttt tctgggtgaa
     1201 gttttgacgc tgaagcctta tatgaaagct cggattaatt tggctcccga aataattcca
     1261 agcgctgaag atggtgtttt tccggcactg gttgctacca ttaaggatat ggctgcaaaa
     1321 attatagcca atgcaggaat aatgcctcca gaagcagttt ttgccatacg taatattgat
     1381 aaccccgttt ttcttattaa ttatgtatgt gtgaattttg gattgaatgt acaggaaaag
     1441 caaagactgc ttgaaattga cgatattctg gatcggggct atcaattgct cgatttgttg
     1501 aacaaggaat ggcaattgct ggaaataaaa atttctattg aaaataaagc caaagaaggt
     1561 atcgatcagc agcagaagca gtatttcctg cagcagcaaa tgaaaaccat tcaggaagaa
     1621 ttgggtggag cttctcccga acaggatatt ctggatttca gagaaaaagc aaaacaaaaa
     1681 aaatggaatg aaaatgtggc tgctttgttt gagaaggaat tgaaaaaact ggaacgtatg
     1741 tcgcctcatt ctccggatta tactacgcag ctgaattatg tggaaacttt attggaattg
     1801 ccatggaatg agtacacaaa agataatttt aatttgaaaa atgcacgtaa aatccttgaa
     1861 caggatcatt tcggcatgga aaccgtaaaa gaacgtattt tggaacattt ggccgtgctg
     1921 aaattgaagg gagacatgaa atcgcccatt atttgtcttt atggcccgcc gggaaccgga
     1981 aagacttcgt tgggcaaatc catagccaga gcgttgaaca gaaaatatgt aagaatttcg
     2041 cttggtggtt tgcatgacga agccgaaatt cgcggtcatc gaagaactta tatcggagcg
     2101 ttgccgggaa gaattatgca gaatattcag aaagcacagt cggccaatcc ggtttttgtg
     2161 ttggatgaaa ttgacaaggt tgctgccgat tataaaggtg atccgtcgtc tgctttgctt
     2221 gaagttctcg accccgagca gaataccagt tttcacgata attatctcga cgtcgatttc
     2281 gatttgtcga aagtgatgtt tattgctaca gccaacaatc tgaataccat tcctcgtcct
     2341 ttactcgaca ggatggaatt gatagaagta agtggctata cacaggaaga aaaagtggaa
     2401 attgcccgac gccatcttgt tcccaaagag ctcgaaaatc acggtttaac aaaagaacag
     2461 ttgattttga cgaagcctgt aatcgaacat ttgatcgaaa attatacgcg tgaatcgggc
     2521 gttcgcgaat tggacagaca aattgcttcg ctcatgcgga aaattgcaaa aaaagtggct
     2581 tccaatgagc cttataatgt gcaacttact gtagatgatt tgaaaacttt tttgggagtt
     2641 ccgcgatata caaaagaaaa atatcaggga aacgattatg ccggggttgt taccggttta
     2701 gcatggacac aagtgggggg cgaaattctc tatattgagt cgagtctaag caaaagtaaa
     2761 tctcctcagt tgactttaac gggaaatttg ggcgaagtga tgaaagaatc tgctgtgatt
     2821 gcattggaat acataaaagc acacgccgaa cagttaggaa ttgatagtga aatgtttgaa
     2881 aaatggaatg ttcacattca tgttcccgaa ggagctattc ccaaagatgg tccgtcggcg
     2941 ggaattacaa tgcttacagc gcttgtttcg gcatttacca aaagaaaagt tcggaaaaat
     3001 cttgccatga ccggcgaaat caccttacgt ggaaaagtat taccggttgg cggaattaaa
     3061 gaaaaaatac ttgcggccaa aagagcaggc ataaccgaat tggttctttg cgaagagaac
     3121 aaaaaagatg tggaagaaat taatgcgtct tatttgaaag gtcttcattt tcactacgta
     3181 aacgatataa tggaagtcat caatttcgct ctattggatg agaaagttag gatggagtaa
     3241 atttaaagat ttgtcaagta tgattagtca atccttaaaa gtttgtttta gtggtaagat
     3301 tataaaattt ttgttgaaat gaaacaaaaa actcttcttt gagttttcac atcatttttt
     3361 tcaaattcca agcttgagta gccaataaag cgttaatttg tatgccgtct tcgccgattg
     3421 gtttgccgaa aatggacaaa atcgttctga tcaaacggca aacggtattc aaaaaacaca
     3481 cttctgcaaa aatgctgaaa ttaaacattc cgtattcaat aattcagtta tcgtttatct
     3541 cgaaattgta caaatgtttg agcaacatcc actctaccct tctccatttg aaacactctg
     3601 gggccattgg caaaatatag ataccaattc cttctcaaag taagaaagta acttcagtct
     3661 attttgtccc ataattgaat taattcatgg ttcatatcta ttataaag
//
LOCUS       DAOTAX010000118         3657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_20355_length_3657_cov_28.912549, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000118 DAOTAX010000000
VERSION     DAOTAX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20355_length_3657_cov_28.912549"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..591)
                     /locus_tag="PLM04_08365"
     CDS             complement(154..591)
                     /locus_tag="PLM04_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03120.1"
                     /translation="MANKINTQINPIFQFTELTPKEYEIMPEEGLYLLRCKLPEDDWA
                     CICKGDMKSELSKQIRHCGIGNWGKDGGFQIYSVAVSDNFKEGNGVSPLFILHMLYCG
                     EGRFAIKKQRQEIAPYKKPKVCIRDGRSRDYPLQNKKPHLLAV"
     gene            1377..1661
                     /locus_tag="PLM04_08370"
     CDS             1377..1661
                     /locus_tag="PLM04_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03121.1"
                     /translation="MERTVRIGADEIILWLRKNRLAAETPNDGITGLGVKILDLIVNK
                     LHGRKVEDSAPSFWDATDTVVQQFGLPKTSAQYEINVNRLTDLYLEISNW"
     gene            1679..3394
                     /locus_tag="PLM04_08375"
     CDS             1679..3394
                     /locus_tag="PLM04_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03122.1"
                     /translation="MNTSTKAKKSPLISFGKFSIIFLMVGLLAHSFDVKAQQNLYDYN
                     DFTPYEKLRCDLATEAMEKIVKVMESLSPYDQMTLGLTYAKSIENSDNEYDLMLAHEN
                     FMTTLFGVSSLTEPETYYEYHVMEEFKSIAKWYFPQRAQVEKKRTSLDDAREKERNSG
                     KETLHRNIKSAFISWAKKGEYEKRDEYLLRLQEQAIGKFDSICMWHCVSQFFNNLDSK
                     GLGYDADEEAYSLRIFHKINNNDDKEQAPVVLTAHMPVEEAKILGKMESGTIVPLNLY
                     RYQSYLCPKTMLFPYRYQGKQYNYYFKGDFESNDPIMVAYNDLNCGNSDLDGIMAGYV
                     FDYNRYMDEMVSLTDSINLMALQTKKKIECLRGVCRRIKTSFQDQNPWTGFVKQVDNA
                     YKNRDFGLSPIEYIQMNYVKEIPLLTSKTDFNDRNVVLSEFMFLAEEFVRTTFSEYSC
                     LFASDKDFIDLFLNENGFDESKLVEIVNMKFSGFISNLGDGQALKGSYDSPIGKEMIN
                     YCNLGYFEEKRTHMEHFEFLPSVSQTIEQLLAVFVSQNRSLEKAKNRQNGSYRIFMLR
                     YIYGK"
BASE COUNT         1181 a          629 c          722 g         1125 t
ORIGIN      
        1 ggggaaacta aagaacgaat agtaattacc caaataccca aagaactaat aggccatggc
       61 ctgttagttc tttttatttg cgcatttatt tgccgtttac catgcgaact ctatattacc
      121 ttgcagtatg taaggtttgt ttgagaactc gcattatacg gcaagtaaat gcggtttttt
      181 attttggagg ggataatcac ggcttctccc gtccctaata catacctttg gtttcttata
      241 aggggcaatc tcctgtcgtt gtttcttaat tgcaaatctc ccttctccgc aatacagcat
      301 atggagaatg aaaaggggag atactccatt tccttctttg aagttgtctg acaccgcaac
      361 ggaatagatt tgaaatccac catccttgcc ccaattccca atgccgcaat ggcgtatctg
      421 cttggataat tcggacttca tatccccttt acaaatacac gcccaatcgt cctcgggcag
      481 tttgcatcga aggagataaa ggccttcttc gggcattatt tcatactctt tcggggtcaa
      541 ttctgtgaat tgaaagatgg ggttgatttg ggtattgatt ttgtttgcca taactttgaa
      601 cttttgatgg attattgttt cttttacgca tatgtataaa tgaaaccctt caaaagttaa
      661 gtggcaaaat gtaaattagt gttatgtacc aaagtgtgta tccacgagac tgaaaacgtt
      721 aatcaatcct ccattgaaat ggcaattata ggcatataaa gactttgaag gttgggaagt
      781 gtattacaaa ctctttacaa cattggatga atggtgtatg gaaaatctga aagatgagca
      841 attggaatac tttttggaaa caatagataa aaaggtgtac ctttgtcgca tctcatagtc
      901 attgttggtc gagatacaaa tgaccattta cgttcgcaaa atcctagaaa accgagtgcc
      961 agccaatagg ttggcactct ttgtatggga tcaaaaagac catccataat atattatacg
     1021 tggcacatag tgagtgaact tatcttgacc gattttgaga aaatgttgcg cttaatcaaa
     1081 aaatgcctat ttttgccata gttcttttgg cggattctat tccgctgaca tgattatacg
     1141 aagcgttatg ctcaacttgc gaatggaaac gtgagaaact tctattgata agcaagataa
     1201 gcgtaatggc tcacgcatat agcgtgggct gattgcgatt tctgcttatc agggttatct
     1261 cacgacctcc attcgaagaa gtagcgtatc agtgccacgc ttcttttatt gttatccgtc
     1321 ctttaggtgc tgaaagggct attaaattgc accctaaaca gataaacaga aaaaacatgg
     1381 aaagaacagt tagaattgga gctgatgaaa taatattatg gcttcgcaaa aatagacttg
     1441 cagcagaaac gccaaatgat ggtataacag gtctaggcgt taaaatactt gacttgattg
     1501 tgaataaatt acatggaaga aaagttgagg actctgctcc gtcgttttgg gatgcaacag
     1561 acacagtcgt gcagcaattt ggtcttccaa agacttccgc acaatatgaa ataaatgtaa
     1621 atcgattaac cgatttgtat ttggaaattt cgaattggta acattaaatc tttttattat
     1681 gaatacttca actaaagcaa aaaaaagccc cttaattagt tttggtaaat tttccatcat
     1741 ctttttgatg gttggattac ttgcccattc atttgatgta aaagcacaac aaaatttgta
     1801 tgattacaac gattttacac cttatgagaa attgcgttgt gatttggcaa ctgaagcgat
     1861 ggagaaaata gttaaagtaa tggaatctct gtcgccttat gaccaaatga cattaggatt
     1921 aacatatgca aagtccattg aaaattctga taatgaatat gatttgatgc tcgctcacga
     1981 aaacttcatg accactcttt ttggagtgtc atccttgaca gagccagaaa catattacga
     2041 atatcatgtg atggaggaat ttaaatctat tgcaaaatgg tactttccgc aaagggcaca
     2101 agttgaaaaa aagcgaactt ccttagatga tgctcgtgaa aaagaaagaa attcgggtaa
     2161 agagactttg caccgaaata tcaaatccgc atttatttcg tgggcaaaaa aaggcgaata
     2221 tgagaaaaga gacgaatacc ttttgcggct tcaagaacaa gcgataggga aatttgactc
     2281 catttgcatg tggcattgtg taagtcagtt ctttaacaat ttagatagta aaggattagg
     2341 ctatgatgca gatgaagaag cctactcatt aaggattttc cacaaaatta ataataatga
     2401 cgataaagag caagcgcctg ttgtattgac cgcacacatg cctgttgagg aagcgaaaat
     2461 tcttggaaaa atggaaagtg gcaccattgt tcctttaaat ctctatcgct accaaagtta
     2521 tttgtgtccc aaaacaatgc tgttcccata tcgttatcaa ggaaaacaat acaactatta
     2581 ttttaagggt gattttgagt caaacgaccc aataatggtc gcttataatg acttgaattg
     2641 tggaaattca gatttggatg gaataatggc tggatatgtt tttgactaca atcggtatat
     2701 ggatgaaatg gtttcattga cggattcaat aaatttaatg gcattacaga caaagaaaaa
     2761 aattgaatgt ttaagggggg tgtgtcggag aatcaaaacc tccttccaag accaaaaccc
     2821 ttggactggt tttgttaagc aggttgataa tgcatataag aatcgagatt ttggattaag
     2881 tcctattgaa tacattcaga tgaattatgt taaagaaatc ccacttttga ctagtaaaac
     2941 agacttcaac gatcgtaatg tagttctttc ggaattcatg ttcttggcag aagagtttgt
     3001 cagaacgact ttttcagaat atagttgtct attcgcttcc gacaaagact tcattgacct
     3061 tttcctcaac gagaatggtt ttgacgaatc caaacttgtt gagattgtca atatgaaatt
     3121 tagtggtttt atctcaaatc ttggagacgg tcaggcatta aaaggttcct atgattcacc
     3181 catagggaaa gaaatgatta attattgcaa cttgggatat tttgaagaaa aaagaacaca
     3241 catggagcat ttcgagtttc ttccttcggt gtcacaaacg atagaacaat tgttagcggt
     3301 ttttgtttca caaaacaggt ctttggagaa agcaaaaaat cgccagaatg gttcctatcg
     3361 tatttttatg ttacgatata tatatggcaa atagattgtt cttaaacaat ccaaatatct
     3421 catcaaaaca aaggcaggaa ttgcagtttt ttgtcctctg taacatatgt tgctctaaga
     3481 attagttcaa aaagatatgc aaagaaatgc tccccgcaga ttccacgggg agcattattt
     3541 caattgccaa aggaatggga gaatctctat tatatcgcaa ataacctctc aaaaaaagga
     3601 tgtttgagga ctacaatccc atacgtttcc tttcttttaa tatctcgtcc tcggtct
//
LOCUS       DAOTAX010000119         3601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_20728_length_3601_cov_32.736323, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000119 DAOTAX010000000
VERSION     DAOTAX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20728_length_3601_cov_32.736323"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLM04_08380"
     CDS             <1..285
                     /locus_tag="PLM04_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HON03123.1"
                     /translation="HSLRMIYNKNSIKAAARLLLAKWYNNVAERQFKSFNTIAATVYE
                     HHDEVLNFFIDRSTNASAESFNAKIKSFRASLRGVADIKFFLFRLTNIYA"
     gene            complement(553..1041)
                     /locus_tag="PLM04_08385"
     CDS             complement(553..1041)
                     /locus_tag="PLM04_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HON03124.1"
                     /translation="MKKNGILILTILLLASFRAGAQTIDKFFDRYANDDRFTYVSVGK
                     GMMNLASTFGANKNMDKNQKEVLDRLTGIKILTLENSRDEKLMNSVLDELDKVIRAGK
                     YETAVRVKDKDETVNIFFRTTGKDQSDMIIVSKEKNEMSIIWMNGKMTKEEMSDIFSQ
                     NS"
     gene            complement(1079..1564)
                     /locus_tag="PLM04_08390"
     CDS             complement(1079..1564)
                     /locus_tag="PLM04_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03125.1"
                     /translation="MKEIDDLLNKYFEGKTTLEEENYLKSYFRNEEIQPEHEVYKTLF
                     HVFDEKAKEKIPQPVDEKNAQPNVKRFWKLTAVIAGIAAAFLMIIWVMPIEKNQDFAI
                     VNGEKIENTQFAQNYALEKLNHVSYKLETNLKPLEAIATVKSRMEPVKKIPEIKKEWK
                     K"
     gene            complement(1614..2123)
                     /locus_tag="PLM04_08395"
     CDS             complement(1614..2123)
                     /locus_tag="PLM04_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HON03126.1"
                     /translation="MDAAQFNSVILPLADQLFRVAKSIVCNDEAAKDAVQELNAKLWE
                     NRSQLDKVQNLTAFAMKSMRNLCLDWLRTRKSDYDVPLDENLIASAPNPHQLMEQKDL
                     TNYVKQLIDKLPELQRTIIHLRDVEGLEISEIAAITSISENAISVNLSRARQKLRTEL
                     MEKSKKEKI"
     gene            complement(2272..3048)
                     /locus_tag="PLM04_08400"
     CDS             complement(2272..3048)
                     /locus_tag="PLM04_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HON03127.1"
                     /translation="MNGIVNLSLVPLRAEDNECSEMISQLLFGETVEIMEWKERWLHI
                     RNLSDNCCGWADRKMITLLSEDEYENLKNNENFRILKAVSFCRNLTLGTTFLLPGGSI
                     LRKFQNGNFTDGTYQYAIHPSDAGMPKPLTGGNIVSLARQYHFTPYLWGGKSALGIDC
                     SGLVQVVFSLCGVLLPRNASQQAEIGEKINSLTQAEAGDLAFFANSAHHIVHVGILMN
                     SHQIIHASGWVKIENIDEHGIISEQTGNYSHTLYSIKRMI"
BASE COUNT         1063 a          673 c          608 g         1257 t
ORIGIN      
        1 cactccttga gaatgattta caacaaaaac agtatcaaag ccgctgcaag acttttattg
       61 gctaaatggt acaataatgt ggctgaaaga caattcaagt cttttaatac aatagctgct
      121 acagtttatg aacatcacga tgaagttctt aatttcttta tcgatagaag taccaatgcc
      181 tctgccgaaa gtttcaatgc taaaattaaa tcctttagag cttctttgag aggagttgcc
      241 gatattaaat tctttctctt cagattgact aatatctatg cttagacaca tgtttttgca
      301 ggtgagccat tacaggcgat aatactaatg taatgcttgc agcagctgca tttaacttca
      361 aaaggatgat gaataaatgg aaaccatctt tttagctcaa acttaaaata caaattgttt
      421 ctcaaaatcg ctttaaataa aaaaacaagc ctggttttcg agcctatgga acatatcttc
      481 agagtttgac tttccttata aattggctaa tgagtaatct gggttaataa acaaaaatat
      541 aaaataagtt tttcaggaat tttgcgaaaa aatatcggac atttcttctt ttgtcatttt
      601 tccgttcatc catattatcg acatttcgtt tttctctttt gacacaataa tcatatcgga
      661 ctgatccttg ccggttgtcc tgaagaagat gttcaccgtt tcatccttat ctttcactct
      721 gacagccgtt tcgtatttcc cggcccgaat taccttgtcc aattcatcaa gaaccgagtt
      781 cattaatttt tcatcgcgtg aattttccaa agttaaaatt ttgattcccg tcaaacggtc
      841 taaaacttcc ttctggttct tgtccatatt tttatttgct ccgaatgttg aagcaaggtt
      901 catcattcct ttgccgacgg aaacgtaagt gaatcgatcg tcattggcat atcgatcaaa
      961 aaatttatcg atggtttgtg cacctgctcg gaaagatgca agcagcaaaa ttgtcaaaat
     1021 taatattcca ttctttttca tattcgtgat tgttaattgt ttaaatgaat tgtttcttct
     1081 attttttcca ttcttttttt atttccggaa tttttttcac cggttccatt ctcgatttaa
     1141 cggtcgcaat tgcttccaaa ggttttaaat ttgtttccaa cttgtagctg acatggttca
     1201 atttttccaa agcataattt tgagcgaatt gtgtattttc aattttttcc ccgttcacaa
     1261 tggcaaaatc ctgatttttc tctatcggca taacccaaat aatcatcaga aaagctgcag
     1321 caattcccgc aatgacggca gttaatttcc aaaaccgttt cacattgggt tgtgcatttt
     1381 tttcgtcaac cggttgaggt attttttctt tggctttttc atcaaacaca tgaaatagag
     1441 ttttgtagac ttcatgttcg ggttgtattt cttcattcct gaaataagat ttcaggtaat
     1501 tttcctcttc aagggtagtt tttccttcaa aatatttatt taataaatca tcaatttctt
     1561 tcattgcaag tcgtttttta ataaattgat aaaacgatta ttcaaaattt ccttcatatt
     1621 ttttcctttt tcgatttctc catcaattct gtccttaatt tttgccgtgc tctcgagaga
     1681 ttaacactta tggcgttttc cgaaattgaa gtaattgctg ctatttccga aatttccaaa
     1741 ccttccacat cgcgaaggtg aataatggtt ctttgcaatt cgggtaattt gtctatcaat
     1801 tgtttcacat agtttgtcag atctttttgt tccatcaact gatgagggtt gggcgcagat
     1861 gctatcagat tttcatccag aggaacatca taatctgatt ttcttgttcg caaccagtcg
     1921 agacataaat tgcgcatgga cttcatggca aaagccgtaa ggttttgcac tttatccagt
     1981 tggctgcgat tttcccacaa ttttgcgttc aactcctgta cggcatcttt ggcagcttca
     2041 tcattgcaga caatggattt tgccacccga aacagttggt cagcaagtgg caaaataacc
     2101 gaattaaatt gtgctgcatc catacattac tgaattctca ctaagttacc aaatttgatt
     2161 catacaccta taatgacgaa aaaccggatg atttcttaca tcaaatactc gatttttttt
     2221 aaaggtaagg agaatttttt ttgaagagag tctgacgatt ttgaaaatga tttaaatcat
     2281 tcttttgatg gagtaaaggg tatgggaata gtttcctgtt tgttcggaaa taattccatg
     2341 ttcatcaata ttttcgattt ttacccagcc tgatgcatga ataatttggt gagaattcat
     2401 tagaataccc acatgaacaa tgtgatgagc agaattggca aagaaagcca aatctccggc
     2461 ctcagcctga gtaagagaat ttattttttc accgatttcg gcctgctgag aagcattgcg
     2521 cggtaacaag acgccgcaaa gcgaaaaaac aacctgaacc aaacctgaac aatcaattcc
     2581 caatgcactt tttccgcccc acaaataggg tgtaaaatga tattgtcgag ctaaagaaac
     2641 gatattgcct ccggttaaag gttttggcat tccagcatcg gaaggatgaa tggcatattg
     2701 atatgtccca tctgtaaaat tcccgttctg aaattttctc agaatgcttc ctccgggcag
     2761 gagaaaagta gttcccaatg tcagattgcg acaaaatgaa accgctttca gaatgcggaa
     2821 attttcgtta ttcttgaggt tttcgtattc atcttccgaa agcaaagtaa tcatttttct
     2881 gtcagcccag ccacaacaat tgtccgaaag attgcgaatg tgcagccatc gttccttcca
     2941 ttccattatt tcaactgttt ctccaaacag aagctgagaa atcatttcgc tgcattcgtt
     3001 gtcttctgcc cttaaaggaa caaggctcaa gttcacaatt ccattcataa atacaataat
     3061 ttatacgttt gatattagta catggcaaaa attgagactt ttatgttata tatctgtaaa
     3121 acagaacaat tatgctaagt tttgggtttg actttgtccg gtgagccaaa aaacaagtga
     3181 gaaaagcgtt taaaaatcgt ccttattcga gctccgacgt atttcgtcgg aatgagtttg
     3241 ggcgatttca gcttttcgag cttaagtttt tcgagtgaag tggacacagt cttgaaagca
     3301 gcatttaagc gaaaacaaaa tcaaatttat ttgatttatg ttgagcgtag ctgcttcaac
     3361 taagtaaatt tttttgctta cttatttgtt tcaagacaaa aaagtgagta gagtttgaga
     3421 caaagcccta ttacgttatt aaatgtctga aaataaagaa tataaatgtt tttacaattt
     3481 ttttgtcatg tactaaggtt taataattaa tatgttagca aaccaaactc catttaatac
     3541 attgaagctt gtttcgttgt gatttatttt ccgaggaaaa tatctctctt ctcttcgttt
     3601 t
//
LOCUS       DAOTAX010000120         3527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_21227_length_3527_cov_26.668491, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000120 DAOTAX010000000
VERSION     DAOTAX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21227_length_3527_cov_26.668491"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1470)
                     /locus_tag="PLM04_08405"
     CDS             complement(124..1470)
                     /locus_tag="PLM04_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03128.1"
                     /translation="MNNMQEIEPVFDFISDEAKIPIHKGQAEVCINKNTYTGNGEVCL
                     ELLPRPNLYFYGYFKGIPANNAVAASLERNKISSFSFNGRQIKGFRLSSGGNIDSQEF
                     NLKWCPESEPIYGVGDESTQISSLVFHLFNFVDLFGTRHSIKQGGSTMHRIEHIDLIC
                     DQWNIALKSLLSTRENIKKLNEEGGCRLTHVGGIKRADSLSFSGKDAKECLEALLFFL
                     SFAKGGWCEPTCAVGFDESDNRVWESWSSPREPWYEPPSWFDRHNGSQLNIFYPGFMK
                     KWKNDDWREALHEVLYWYLNANYSPRGIEAGIILTQAAIERLSYEYTVKDKRLLVLNG
                     FKDLWASDKFRILFSSLGIPLEIPNETPEILRLAKDLNWLDAPHALTEIRNSLVHPEH
                     KRRRQLDSIYYEAWNLGLWYLEMGLLAVCDYSGTYGNRLKQRYVGQVENVPWIKKQ"
     gene            complement(1476..2360)
                     /locus_tag="PLM04_08410"
     CDS             complement(1476..2360)
                     /locus_tag="PLM04_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HON03129.1"
                     /translation="MEILNQYSNALMAIMSTLLVIITLLYVAFTWRMVKEMQITREAD
                     LRPYIIIDILLADQKFHLLVKNIGKTVAESVTFNIDKNIENMWKNTIGEMPLFKEGVA
                     FFPPGREFVVTLGPTWLFLNKKEEIKYPLIFTIVVKYKYFGNISKSEPTTINLDEYMN
                     TMSFPDSFAKSIVNIGASISNGLNSIAKNTEKISKIEEISSPTGIDVSQGTLFRLADI
                     LRENSKHKIKFDLNLTTLMELVELLGVDLKFAEKIIMRRYSDGYFRSFDDLKNIEGIT
                     DELIDNLKKRTFICTPKF"
     gene            complement(2367..>3527)
                     /locus_tag="PLM04_08415"
     CDS             complement(2367..>3527)
                     /locus_tag="PLM04_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HON03130.1"
                     /translation="DAVIGNPPYSAKKSLETKILMKFFSNVEYKCDPYAFFIEQGMNK
                     LKIKGYLGFIVPVTWMTNYYYMKIRKMLIDSDSLDKIVLIDGLVFENANVDTCMLFLN
                     KNGVLTGKFKWLQTDSAHLDKSLSERDYSTVRAEERYDIVPVADDDWNKVRNKIDKLS
                     VKLGSIAKISLGMKLRANDEFVAFEKGTKHPDGIYFGADISRYGLCTPRRFFDFSKAV
                     IVGGTKNPKIQRATPKILIQAIRNLSLKRRIVAAFEKEGDCFVGTVNAVTLLSQSYNI
                     KYILGIINSTLANTYFRKRFTTISLTSAFLGQIPIPLISISDSINQKRHDKIIQLVDQ
                     MLNLNKQLAEAKTDHEKTLIQRQIDATDKQIDKLVYELYELTPEEIKIVEEK"
BASE COUNT         1049 a          699 c          601 g         1178 t
ORIGIN      
        1 tctaggatgg cgatttcttc ggagttaaat cgtatagctc gtaaaccagt ttgtcgattt
       61 gtttgtcggt agcgtcaagc tggcgttgga ttaatgtttt ttcatggtcg gttttggcgt
      121 cggctattgt tttttaatcc acggaacatt ctcaacttgc cccacatatc tttgttttaa
      181 tctattccca taagttcctg agtagtcgca aactgccaga agacccattt ccaaatacca
      241 caaaccaaga ttccaagcct catagtaaat agaatcaagt tggcgacgtc tcttatgttc
      301 ggggtgcacc agagagttac ggatttccgt caatgcatgt ggagcatcca gccaatttaa
      361 gtctttagca agcctaagaa tttcaggtgt ttcgtttggt atttcgagcg gtattccgag
      421 tgacgaaaat aaaatacgga atttatcgga tgcccaaaga tctttaaacc cgttcagcac
      481 aagaagcctt ttgtctttga ctgtatattc gtatgaaagc ctttcgattg ctgcttgtgt
      541 taaaatgatt cccgcctcta ttccgcgcgg tgaataattt gcgtttaaat accaatacaa
      601 cacttcatgt aatgcttcac gccagtcatc atttttccat tttttcatga atccggggta
      661 aaaaatattc aactgtgaac cattatggcg atcgaaccaa gaaggtggct cataccatgg
      721 ctctctcggt gacgaccaag attcccaaac acgattatct gattcgtcaa atcctacggc
      781 gcatgtcggt tcacaccaac cgccctttgc gaaagataga aaaaaaagaa gtgcctccaa
      841 acattctttc gcatctttcc cagaaaaaga taaactgtca gccctcttga tgcctccaac
      901 atgggttaaa cgacatccac cttcttcatt tagctttttg atgttctcgc gtgttgaaag
      961 cagcgacttc aatgcaatgt tccattgatc gcaaatcaaa tcgatatgct caatgcgatg
     1021 cattgtcgaa ccgccttgtt ttattgaatg ccgagtccca aacaaatcaa caaaattgaa
     1081 taagtggaaa acaagtgaag atatttgcgt ggattcatct ccaaccccat aaatcggttc
     1141 cgactctggg caccatttga gattaaactc ctgagaatct atgttgcctc cgctacttaa
     1201 gcgaaaacct ttaatttgac gtccattgaa ggaaaaagaa gagattttat ttcgttcaag
     1261 actagcggcc acagcattat ttgcaggaat acccttaaaa tatccgtaaa aatataaatt
     1321 aggacgtggc aataattcta aacaaacctc accgttacca gtatatgtat ttttattaat
     1381 gcatacctca gcctgacctt tatgaattgg tatcttcgct tcatcgctga taaaatcaaa
     1441 gactggctct atttcttgca tattattcat acttcttaaa attttggtgt acaaatgaaa
     1501 gttcgttttt ttaagttatc aataagctcg tccgtgatcc cttctatgtt cttaagatca
     1561 tcaaaagacc gaaagtatcc atcagaatac cttcgcatta taattttctc tgcaaactta
     1621 agatcaactc cgaggagttc aaccaattcc ataagcgtag taagattcag atcaaactta
     1681 attttgtgct tcgagttttc tctgagaata tcggcaaggc ggaatagtgt cccttgggaa
     1741 acatctattc ctgttgggct tgatatttct tcaatcttgg aaatcttttc ggtgttttta
     1801 gctatagaat taaggccatt gctaatggaa gcgcctatgt ttacgataga tttagcaaag
     1861 ctatccggaa aagacatagt attcatgtac tcgtctaaat taatagtagt tggttcggat
     1921 ttcgagatat taccaaaata tttatatttg actacgatag taaaaattag cggatatttt
     1981 atttcttctt ttttgttaag gaataaccat gttggcccca aagttacgac aaactcccgt
     2041 ccaggaggaa agaatgcaac tccttcttta aaaagaggca tttcacctat cgtgtttttc
     2101 cacatatttt caatattttt atctatatta aaggtgacac tttcggctac ggtttttcca
     2161 atatttttaa caagaagatg gaatttctgg tcagcaagaa gaatgtcaat tatgatgtac
     2221 ggacgcagat cagcctcgcg agttatttgc atttccttta ccatgcgcca cgtgaacgca
     2281 acgtagagca atgttatgat taccaacaac gttgacatta tagccatcag cgcattggaa
     2341 tattggttca aaatttccat ttcagtttat ttttcttcta caatttttat ttcttcaggt
     2401 gttaactcat agagttcgta aaccagctta tctatttgct tgtcggtggc gtcgatttga
     2461 cgctggatta atgttttttc atggtcggtt ttggcttccg ctaattgctt attcaaattt
     2521 agcatttggt caacgagttg tatgatttta tcatgtcgtt tttgattaat agaatcagaa
     2581 atacttatta gcggaattgg aatttggcct aaaaatgctg atgttaaaga tattgttgta
     2641 aatcttttcc taaagtatgt attagctaat gtcgagttta ttattcccaa aatatactta
     2701 atgttataac tttgtgacaa caaagtaact gcgttaaccg ttccaacaaa acaatcacct
     2761 tctttttcaa aagccgcaac aattctacgt tttaaactta aatttcttat tgcttgaatt
     2821 aaaatctttg gagtagccct ttgaatttta ggattttttg ttcctccaac aataactgct
     2881 ttagaaaaat caaaaaatcg cctaggggta cataaaccat aacgagatat atctgctcca
     2941 aaataaatac cgtcagggtg ttttgtgcct ttttcaaaag caacaaactc gtcgtttgct
     3001 cttaatttca taccaagaga tatttttgca attgaaccta attttacgga taatttatca
     3061 attttatttc gcactttatt ccaatcatca tccgcgacag gcacaatatc ataacgctct
     3121 tccgcacgaa cagttgagta atctcgctca ctaagggatt tgtcaagatg agcagagtct
     3181 gtttgaagcc atttaaattt tccggtcaaa actccatttt tatttagaaa caacatacaa
     3241 gtatcaacgt tagcattttc aaaaactaat ccgtctatta atacaatttt gtcaagtgaa
     3301 tcactgtcaa ttagcatctt tcgtattttc atatagtaat agttagtcat ccatgttacc
     3361 ggaacaataa atcctaaata acccttgatt ttcaatttat tcattccttg ctcaataaaa
     3421 aatgcatatg ggtcacattt atattcaaca tttgaaaaga atttcataag tattttagtt
     3481 tctagagact ttttcgcgct atacggcgga ttgccgatga ccgcgtc
//
LOCUS       DAOTAX010000121         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_21569_length_3474_cov_33.996490, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000121 DAOTAX010000000
VERSION     DAOTAX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21569_length_3474_cov_33.996490"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..1661
                     /locus_tag="PLM04_08420"
     CDS             393..1661
                     /locus_tag="PLM04_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HON03131.1"
                     /translation="MKFFLRLNKLLSEISAKGFFHLLSANVLIQLVAFASQLFVAGIL
                     LPEDVGRIKIITTFLSVFSIIGGMGLHASTLKLCSENRSQKEISELFSSGVVFTIVST
                     IVFYGLALVLNSFGFFSSDAIIRRLIPLGLFPLITNSAFMVLVAYFQASKQIKIMSRI
                     TVANKLIAIVAIVLFTWWFGINGYYVAYNLSFIVMFFVGFRLLRKDFTFSKDRESVKH
                     NFKTHLVYAKPSLMSNLLSEISAYADIFVINYLLKDMKEIGYYSFALTLTVVLRVFPF
                     TVQQIASPYFSGLAHDRSHYLQVFKKYNRQLYLVVGVTLLLAILAGPFLIRIIFAGKY
                     DPAIPYFVLLSVGWSIRLLNQLQSAAIFGLGKIQYNAWSSFISLIFNVAVYIVFVRLY
                     GVIGAAWASIPAGVVMTLTSGLFLRKAMRE"
     gene            complement(1753..3153)
                     /locus_tag="PLM04_08425"
     CDS             complement(1753..3153)
                     /locus_tag="PLM04_08425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HON03132.1"
                     /translation="MNKRKLLYRYLISDTLASLVVWVVFMIFRKIVNDAAIFENVQIF
                     FPNYNYLSSLILFPLSCITVHYLSGFYLYPEKHSGIKIFFTTFVASLIISIVIFFVLM
                     LDDIVVSYKYYYYSLAVLFGLLFVFTYLPRLIIYSNVKSNYRSKKWTIPTVIIGTGQN
                     AIKIGNELAKAAPEKTVVGYVETNHGKLISQEMILGNLHQIEKIIEKYNIQEAIIALD
                     QTDDYQLFSIINSLYRFNIDIQFTPRLYEILTGSARIDNLSISPLVSITDIKMPDWEQ
                     SVKRTFDIGFSLLALVLLSPVLLYFMIRIKTDSKGPVFYLQERIGRYGRPFKIIKFRT
                     MFEGAENGIPRLSNPNDNRITNIGKKLRKYRIDEIPQFINVLLGHMSIVGPRPERPYY
                     IQQIVQEAPYYCLLYKIRPGLTSWGPIRIGYSDSIEKMIERLNYDIIYLESMSLITDL
                     KILLLTLEIIFKGKGV"
BASE COUNT          990 a          646 c          633 g         1205 t
ORIGIN      
        1 tcagacatgt gggttccatc agacctttaa aaaataaatc atattctgat gaaaactata
       61 accactcctt ttcaaatgct tgaactccga aaatccttct gtcagtctca acgccttgaa
      121 acctttcaaa atatttatgc tctctaattt attttcattg aaatctgttc ctgtttcaga
      181 aattttttcc ttaattttgt ccggtagcat attttttctg ttttgtaatt atacagctaa
      241 tatactcatt tttaatgaga tattagataa aatatgctac ttttttactc ttttttctct
      301 tttttctctc ttattttcag tcctttctga aatgcgaaac tttagtaaat aaatgtatgt
      361 gtcagaaaga atataggcaa aagatgaaaa acatgaagtt ttttctccgg ctcaataaac
      421 ttttatccga aatttctgca aaaggctttt tccatttgct gtcggccaat gttttgattc
      481 aactggtggc tttcgcttcg cagctttttg tagccggtat ccttttgccc gaagacgttg
      541 gacgaataaa aattatcacc acttttcttt ctgttttttc catcatcgga ggaatgggtt
      601 tgcatgcttc taccttgaaa ctttgttccg aaaaccgttc acaaaaagaa atcagtgaac
      661 ttttcagttc cggtgtagtg tttaccattg tttccaccat tgttttttac gggttggcac
      721 ttgtattgaa ttcttttgga tttttttctt ccgatgccat catccgcagg cttattccgc
      781 tgggactttt ccctctgatt acgaattcgg ctttcatggt tttggttgct tattttcagg
      841 ccagcaaaca aatcaaaatc atgtcccgca taactgttgc caataaactg attgccattg
      901 ttgccattgt gttgttcacg tggtggtttg gaattaatgg ctattatgtg gcttacaacc
      961 tgagttttat tgttatgttt tttgttgggt tccggttgtt gcgaaaagat tttacttttt
     1021 ccaaagacag ggaaagtgtc aaacataatt ttaaaacaca tttagtttat gcaaaacctt
     1081 cattgatgtc caatctgctt tccgaaattt ctgcttatgc cgatattttt gtgatcaact
     1141 atttgttgaa agacatgaaa gaaattggtt actacagttt tgcattgacg ttgactgtag
     1201 tgctgcgtgt ttttcctttt acggtgcaac aaatagcatc accctatttt tccggacttg
     1261 cccacgaccg cagccattat ttgcaggttt ttaaaaaata caaccgacag ctttatttgg
     1321 ttgttggcgt tacattgctg ctggccatat tggcagggcc ttttcttatc aggattattt
     1381 ttgcgggaaa atacgatccg gccattcctt atttcgtcct gttgtcggta ggttggagta
     1441 tccgcttgct gaaccaactt cagagtgcag ccatttttgg attgggaaaa attcagtaca
     1501 acgcttggag cagttttatt tcgttgatat tcaacgtagc cgtttatatt gtttttgttc
     1561 gcttgtatgg cgttattgga gcggcatggg caagtattcc ggctggagtt gtcatgaccc
     1621 tcacatcggg tttgtttctc agaaaagcaa tgcgggaata actggttcgg tattttttgt
     1681 ttattgattt acatcgacta ggcgaaaaac tttgacaaat tttggttctt ttattcaaat
     1741 ttttgtacaa gtttacactc cttttccctt aaaaataatt tccaaagtaa gaaggagaat
     1801 tttcaggtcg gtaatcaatg acatgctttc caagtagata atgtcgtaat tcagccgttc
     1861 gatcattttt tcgatactgt cggaatagcc aatgcggata ggtccccacg aggtgagtcc
     1921 cggccgaatt ttgtataaca gacagtaata gggagcttcc tgaactattt gctgaatata
     1981 gtatggcctt tcgggacgcg gaccaacaat gctcatgtgt cccaataaaa cgttgataaa
     2041 ttgtggaatt tcgtcaatgc gatactttct caactttttc ccaatattcg ttatccgatt
     2101 atcgttggga ttgctcaatc ggggaatgcc gttctccgca ccttcaaaca tggttcggaa
     2161 cttaataatt ttgaatggcc gaccataacg tccgattctt tcttgaagat aaaaaacagg
     2221 gcctttggaa tccgttttaa tccgaatcat aaaatacagc aaaacgggcg aaagcaagac
     2281 caaagccaac agcgaaaagc caatgtcgaa tgtccgtttg acgctttgtt cccaatcggg
     2341 catttttatg tcggtaatac ttaccaacgg actaatgctt aaattgtcga tgcgggcact
     2401 tccggttaaa atttcataca gtcgtggcgt aaactgaata tcgatattga agcgatagag
     2461 ggaattgatt attgaaaaaa gctgataatc atcagtttga tcgagtgcaa taatggcttc
     2521 ctgtatgttg tatttttcga taattttttc gatttgatgt aaattgccaa gtatcatttc
     2581 ctgtgatatc agctttccat gattagtttc cacatatccc accacagttt tttcaggagc
     2641 tgcctttgcc agctcattcc ctattttgat ggcattttgt cccgttccaa taataaccgt
     2701 cggaatagtc catttttttg aacgatagtt gcttttaaca ttggaataaa taataagacg
     2761 aggcaaataa gtaaacacaa acaacaatcc gaacaacacg gcgagagaat aatagtagta
     2821 tttgtaagat accacaatat cgtcgagcat caacacaaaa aaaatgacaa tggaaataat
     2881 caatgaagct acaaaagtgg taaaaaatat ttttatgcct gaatgttttt caggataaag
     2941 ataaaatccg gaaagataat gaactgtaat acaactcaac ggaaaaagta tcagactgga
     3001 aagataattg taattcggaa agaaaatttg aacattttca aaaatagctg catcattcac
     3061 tatttttcgg aaaatcatga acactaccca caccaccagt gaagccagtg tatccgaaat
     3121 caaataacga tataacagtt ttctcttgtt catccgctcg ctgatgagga aatttctaaa
     3181 ttttgaattc tattacttta taattgtttt caatattttg cgacacttat catctctcaa
     3241 ttttttataa acgaaaatta gtattcttta ttttcttttc accgctttat aattattact
     3301 ttaatacatg gcaaaaattg aaatatttgt tttatatatc tgtaaaacac gacatatcag
     3361 ccaagcactt gggttgacct tgtccggtga gccgaaaaac aagtgagaaa agcgtttaaa
     3421 aatcgtcctt gtttgagttc cgacgtattt cgtcggaatg agtttgggcg attt
//
LOCUS       DAOTAX010000122         3326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_22704_length_3326_cov_35.180373, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000122 DAOTAX010000000
VERSION     DAOTAX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22704_length_3326_cov_35.180373"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..249)
                     /locus_tag="PLM04_08430"
     CDS             complement(61..249)
                     /locus_tag="PLM04_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03133.1"
                     /translation="MFLNNTFSGREKKPYLNYGDTFKFQYEKIERLTFTDLFEKINND
                     IQNINDEINNIKGQIPSS"
     gene            complement(784..1068)
                     /locus_tag="PLM04_08435"
     CDS             complement(784..1068)
                     /locus_tag="PLM04_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03134.1"
                     /translation="MNNIDTTIEKIKASPLFYLSLSSRELFHSNFWFWLSTLNKIETA
                     KLFSDKTITNDITFKRGHNQINGEFKASVDLYVADKIAIENKVKDFPTSS"
     gene            complement(1201..1722)
                     /locus_tag="PLM04_08440"
     CDS             complement(1201..1722)
                     /locus_tag="PLM04_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HON03135.1"
                     /translation="MKKILFLASLIIISLQIGNCQKRFDSIPSIEIETLSGAKTNLQV
                     CYGIEKNAMIVFSWGCCWGPPCQRVLDSLEKYFLDWEKKYNIKIFSINYKVCKGNELH
                     ECIKKVLDDKRWTFPFYWDNDDHSAYHYFNVNYNMCPQFTIIDYRNKTFETSSSSNSF
                     FVEDVERNLSKLK"
     gene            complement(1887..2582)
                     /locus_tag="PLM04_08445"
     CDS             complement(1887..2582)
                     /locus_tag="PLM04_08445"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HON03136.1"
                     /translation="MKNILIIIVVAVAFLSSCTTTAPIAKSVAYKGMYDEKPLTVLLM
                     PPINRSTNVEAKEYFYSTLYIPIANAGFYVISPFLSMEILKKESAYDAELFLNSPLTK
                     FGEVFGADLALFTIIHKWDKSGLAAKVYIQVEYIIKSTKTNEVVYTRTGDVTYDASVS
                     TGVGGAWSGLADIAASAINTAATKYVNVARACNAYTFKDLPAGKYSPIYGTDGEQLAG
                     EKLFKLNLNSKYK"
     gene            complement(2579..2923)
                     /locus_tag="PLM04_08450"
     CDS             complement(2579..2923)
                     /locus_tag="PLM04_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HON03137.1"
                     /translation="MKKILFISITVLMLASCTTQKPLYTWGKYEITSYNYLKNSDEKS
                     TQQLIETYQQIIEKQKGSRGVVPPGVYADYGFILLQANKTEEGKAMLLKEVALYPESK
                     IFIDRILKIIEE"
     gene            complement(2920..3252)
                     /locus_tag="PLM04_08455"
     CDS             complement(2920..3252)
                     /locus_tag="PLM04_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03138.1"
                     /translation="MKKILFVIAIGLIVLTGCTGVKTLSTGLENEAFIEFIGKPSNYS
                     GGVDVNIDDKTTFKAEVNKDHADRPKGKVYAISTGSHMITVSYNNNVIFKKQIFVSAQ
                     ETKKIMLP"
BASE COUNT         1004 a          565 c          527 g         1230 t
ORIGIN      
        1 aatagtattg aagggacaag aaatactgct taacagaggt gctttacata ggaacgcctg
       61 ttagctgctg ggtatttgtc ctttaatgtt gttgatttcg tcattaatgt tttgaatgtc
      121 gttgtttatt ttttcaaaca agtcagtaaa agttaatctt tcaatttttt catattgaaa
      181 tttaaaagtg tcgccatagt ttaggtaagg cttcttttct ctacccgaaa atgtattgtt
      241 gaggaacaaa ttatttccaa ttaaattttc tgcgaatttt cctgctttcc ttccaattac
      301 gaattttctg tattgtatat cctcaatctg aacacctaac ttataaccgt cttgtaagtc
      361 aataacaaag tcaatagttg ctttttgatt gttgatgtcg tatgctgttg ctacgttata
      421 agtgtttgat ttgccaaaat ggattagtaa gtgactagct cgtaatttct gatagccctc
      481 tcaaagtttt atttcgttta gttttccgta tagaactttg tttaatgata ttgcaaaatc
      541 gtattcctgc tttatttcca actgttcgga taattcggat aaattaaatg caaactgttt
      601 gtagtctgat attaagcttt ggtagtaagg gtcattagag aatttgtttg gttctatata
      661 atcagtaata gttctatacg ttttgacagt ccaaccttca aatgataagt tatttgtcca
      721 aaataaagtt gttagcacaa actcaatatc cgtttttcca aatgattgtt taattcgttc
      781 gagttatgat gacgttggaa aatctttgac cttattttct attgcaattt tgtctgccac
      841 atacaagtca acactagctt taaattctcc gttgatttgg ttgtgtcctc ttttgaaagt
      901 gatgtcgtta gttattgttt tgtcgctaaa aagttttgct gtttcaattt tattcaaagt
      961 cgataaccaa aaccaaaaat ttgaatggaa taactctctg gaagaaaggg aaagataaaa
     1021 tagcggactt gctttaattt tttctattgt cgtgtcgata ttattcattt ttcttattgt
     1081 gtcgttgttt taccttgcag ctaatgggtg gctgctaaat gcagcgaagg cagcgacttt
     1141 gcttgcaact tgccgcaggt tttgttgcgt tttgcagctc gttagtggca gaaatattaa
     1201 ttattttaat tttgataaat ttcgctcaac atcttctaca aaaaaactat tggaactact
     1261 agatgtttca aatgttttat tcctataatc aataattgta aattgagggc acatattata
     1321 atttacattg aaataatgat atgcggagtg atcatcatta tcccaataaa atgggaatgt
     1381 ccatctttta tcatctaaca ccttcttaat acattcatgc aattcatttc ctttacagac
     1441 tttataatta atcgaaaaaa ttttaatgtt atatttcttt tcccaatcca aaaaatattt
     1501 ttctaacgaa tctaaaactc tttggcatgg cggcccccaa caacatcccc aagaaaatac
     1561 aatcattgca tttttttcaa ttccatagca aacttgcaaa tttgtttttg caccagaaag
     1621 agtttcaatt tcaatagatg gaattgagtc aaatcttttt tgacaattac caatctgaag
     1681 agatatgata attagtgaag ctaaaaataa aatttttttc ataatgttta tatttaatgt
     1741 ttttgccact aacggtcgag tgtatgtgcc gtaagggact gcagagcagg ttcctgtcca
     1801 ccgacacaaa agtttgagcg ggttacaacg cttgaatacc caccccttat ggcacataca
     1861 ctttgttagg caacgtaatt cattgtttat ttgtactttg agtttagatt tagtttaaat
     1921 aatttctcac ctgcaagctg ttctccgtca gtgccgtata ttggactgta tttaccagct
     1981 ggcaaatctt tgaaagtata ggcattgcaa gctcttgcaa cattaacgta ctttgttgct
     2041 gctgtgttta tagctgatgc agcaatatca gctaacccac tccaagcgcc accaacaccc
     2101 gttgatacgg aagcatcata agttacatca ccagttcttg tataaaccac ttcatttgtt
     2161 ttagttgatt tgataatata ttcaacttgt atatacactt ttgctgcaag tcctgatttg
     2221 tcccatttat ggattattgt aaataaagca aggtcagcac caaatacttc accaaactta
     2281 gtcagaggtg aatttaaaaa aagttcagca tcatacgcac tttctttttt tagaatctcc
     2341 attgaaagaa aaggagaaat aacgtaaaaa cctgcgttag cgattggaat atataaagtt
     2401 gaataaaaat actcctttgc ttcaacattt gtactcctat ttattggagg cataagcaaa
     2461 acagttaaag gcttttcatc atacattcct ttataggcaa cagattttgc aattggggca
     2521 gttgttgtac aacttgacaa aaatgcaact gcaactacga ttataataag gatatttttc
     2581 attcttcaat tatttttaag attctgtcaa taaatatttt tgattctgga tataaagcaa
     2641 cttcctttaa caacattgct tttccttctt ctgttttatt agcttgaaga agtataaaac
     2701 cataatcagc gtaaacacca ggaggtacaa cacccctaga acctttctgt ttttcgataa
     2761 tttgctgata ggtttcaatt aattgctgag tagatttttc atcactgttt tttaaatagt
     2821 tgtaactagt gatttcatat ttaccccaag tatatagggg tttttgtgtt gtgcacgatg
     2881 ccaacattaa aacagttatc gaaatgaata gtattttttt catggcagca ttattttttt
     2941 agtttcttgt gctgaaacaa aaatttgttt cttgaaaatt acattattgt tgtaggacac
     3001 tgtaatcata tgcgaccctg tggatatagc ataaactttg cccttaggtc ggtctgcgtg
     3061 gtctttgttt acttctgctt taaaagtagt tttgtcatca atattaacat caacaccacc
     3121 agaataatta cttggtttcc caatgaattc aatgaatgcc tcattttcta accctgtcga
     3181 caatgttttt actcctgtgc aaccagttag aactatcaaa ccaattgcta ttacaaaaag
     3241 tatctttttc attacttaat ctcccttata aaatagttac ttaaattttg tttgtcaatg
     3301 ctaccttcag aaaatgaaac catgga
//
LOCUS       DAOTAX010000123         3235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_23516_length_3235_cov_29.613208, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000123 DAOTAX010000000
VERSION     DAOTAX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23516_length_3235_cov_29.613208"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLM04_08460"
     CDS             complement(<1..785)
                     /locus_tag="PLM04_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HON03139.1"
                     /translation="MKKIALFTLLCLSINLFSEVITWQGPVGFSSTKYYEVKVNGIPV
                     PVYDTPIASYAIFDFTGKVDVEVSTMFDVRWVDIRPLRTQIKPHYTSDNSFRFNLDKP
                     QNLSLELNGRIRQQPLFIFAGKPETDIPSKSDPNVIWFEGGKLYKNVNLELNDNQTVY
                     IEGGAVVQGIIIANGKKNIKICGKGILDGTYNRQTAGNDNRFIKLVDCENIDINDITL
                     HNGTTWQVHLRHCNNSRINGIRIVSENGGDDGMDIVRCTNVVI"
     gene            complement(1062..1340)
                     /locus_tag="PLM04_08465"
     CDS             complement(1062..1340)
                     /locus_tag="PLM04_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03140.1"
                     /translation="MMQQLKVDKNHFSAWNKNYATGFSALPAGLAYDNVLGRKGDWAI
                     FWASNSSGEHYAWSVEMDNFYPVLSGNSHLMLTNTYLKKCFFSSLRKE"
     gene            complement(1337..1531)
                     /locus_tag="PLM04_08470"
                     /pseudo
     CDS             complement(1337..1531)
                     /locus_tag="PLM04_08470"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
     gene            2093..2344
                     /locus_tag="PLM04_08475"
     CDS             2093..2344
                     /locus_tag="PLM04_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03141.1"
                     /translation="MDTDDLSGEAYWAILGEAEKLTENLTLRYGLLSYECKNETEYLE
                     KAKKLSYQIMKLNDNALYDLFFGDPPEKKDLQATLKKNY"
     gene            2389..2889
                     /locus_tag="PLM04_08480"
     CDS             2389..2889
                     /locus_tag="PLM04_08480"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HON03142.1"
                     /translation="MTSKEEIKQKEQQLLEMTKAFCSKKLNGVYAQLCEKLIKKMGRK
                     RDVPFQRGKLEIWAAAVIQAIGSINFLFDKSFEPYLSTDEINQYFGTKNSTVSSKARE
                     IKEMFRLQYYDPEFSTKSMTDSNPFNNMVMVNGMIVPLDILPENLQQEVKAARARGED
                     IEFYTE"
BASE COUNT         1063 a          554 c          571 g         1047 t
ORIGIN      
        1 tcaattacta catttgtaca gcgtacaata tccatcccgt cgtcgccacc gttttcactc
       61 acgatacgta ttccattgat tcgactattg ttacaatgcc gcagatgtac ttgccaggta
      121 gttccgttgt ggagagttat atcgttaata tcaatatttt cacaatcgac taactttata
      181 aaacgattgt cgtttccggc ggtttggcga ttatatgttc catctaaaat tcctttgccg
      241 caaattttaa tatttttttt gccattggca ataataatac cctgtaccac agcgccccct
      301 tcgatataaa cggtttgatt atcgttcaat tcaagattta catttttata gagttttcca
      361 ccttcgaacc atattacatt cggatctgac tttgaaggga tatctgtttc gggctttcct
      421 gcaaaaataa ataatggttg ctgacgaata cgcccgttta gctcaaggct aagattttgt
      481 ggcttatcca ggttgaatcg aaaggagtta tcactcgtgt aatggggttt aatttgtgtg
      541 cggagaggtc gaatatccac ccaacgaaca tcaaacatgg tgcttacttc aacatcaact
      601 tttccagtaa aatcgaatat ggcgtatgat gcaattggcg tatcgtaaac gggaacaggg
      661 attccattta cttttacctc gtagtatttg gtagatgaaa aaccaaccgg accttgccat
      721 gtgatgactt cactaaacaa attgatgctt aagcatagaa gtgtgaataa tgcaattttt
      781 ttcatttttt tgtattgtaa gatttctgat acaaaaataa gtcatttgtt ttttaaaaaa
      841 ctattttttt ttcacatatc atgatacttt tacgactatt ttttttattt atttgtgttg
      901 ctttttgttg ttttagtatc ataattttcg ggtctgatta ctgtatattt cctctgcgaa
      961 tttcagtctt ttttctatag aagcttataa taggcactaa aggatggttc aaggacaaag
     1021 agccatttag cagatgagat aagacttttg aaaattattt cttactcttt acgcaacgaa
     1081 ctgaaaaagc attttttcag atacgtattt gtcaacatca aatgggaatt tccactcaaa
     1141 accggataaa aattatccat ttcaacactc catgcataat gttctcctga ggaatttgac
     1201 gcccagaata tcgcccagtc gcctttacgc ccaagcacat tatcgtaagc caatccggct
     1261 ggaagtgcac taaaacccgt agcataattt ttattccacg cagaaaaatg atttttgtca
     1321 actttaagtt gttgcatcat gtccagattg tcgtacaatt tgaagagctc ttcccagtct
     1381 cttttgtagc tacatgccag cagtcaggac aaattccctg attggacaaa cagtcgtaca
     1441 tcattacttc gtcgaatgta taaagtccac cataaacgat acagtttgtt gaatcgttat
     1501 tgtagcaggt tttttccaca atgctgttat tttgttgctt ggtgttttga acagacctac
     1561 attcatgttt ttagtcatcc acgaagttcc gtttattgtg actgtaggat atttaattca
     1621 agtacgagca tataagtaat cgtgtagtct tttgtaatct gggctttagt atatgactaa
     1681 aattgagaca tttattatct gtaaaacagt acatataagt caagttctcg ggttgacctt
     1741 cactggtgaa ccgaaaaatc agtgaagttg gcgttaaaaa atcttctctg tttgagcgac
     1801 ggaggagcgg gtgtagaaga ttttagccaa caaacgtcca tttttcttgt agaaactttg
     1861 catgcaacgt ctcgctgaaa acaataaaaa agaacatgaa gtttagaaca aagtcccgtt
     1921 attaaatatc tgaaaataaa aaacataaat gtttctacaa tttttgtgtc atgtactaag
     1981 aaaaatactt tatttttgtt tgaaaattaa atgtatttta gttctcataa gctaatattt
     2041 tttgaaaaaa agcgtgtata aatagtttta acgagtaaaa taaatctttt ttatggatac
     2101 agatgattta tcgggagaag cctattgggc tattttagga gaagcagaaa aactgactga
     2161 aaatttaact ttgcgatatg gcttactgtc ttatgaatgc aaaaatgaaa ccgagtacct
     2221 tgaaaaggca aaaaaattat cttatcaaat aatgaaactc aacgataatg cattgtatga
     2281 tctctttttt ggggatccgc cggaaaaaaa agatttgcaa gccaccttaa aaaaaaatta
     2341 ttgacaacat agaaaaagtt caaaaaacac ccattcaaaa tcgacattat gacttctaaa
     2401 gaagaaatta aacaaaaaga gcagcaattg ctggaaatga cgaaagcatt ttgttcgaaa
     2461 aaattaaatg gcgtgtacgc acaactctgc gaaaaactga ttaagaaaat gggacgaaaa
     2521 agagatgtcc cctttcaaag aggaaaactc gaaatttggg cagccgccgt gattcaggcc
     2581 atcggttcca tcaattttct gttcgacaag tcgtttgaac cttacctgtc aacggatgaa
     2641 atcaatcaat actttggaac gaaaaattca acggtatcca gcaaagctcg cgaaataaaa
     2701 gagatgttcc gcttgcaata ttatgatccg gaattttcta caaaaagcat gacagactcc
     2761 aatccgttca acaatatggt tatggttaat ggtatgattg tgccattgga tatattgccc
     2821 gagaacctgc agcaagaagt caaagcagcc cgagccagag gcgaagatat tgaattttat
     2881 acggaataaa aaggataggg attcatagtt ttatcggcag gtgttggggt gtacaataag
     2941 cgaatgtttt ccttttttac tttccaatat atcaatcaat tgacaactta taaatatttt
     3001 agtgatttgt tttgaattat aaaattgttc tgtaaatttg tgaaataaac ttttgtttca
     3061 taaaaaaaca tatacgcacg cgtatcacaa aaaagtttac agaaaataaa atcgccgttt
     3121 ggtgatttat aagaatttga taaagttgtt attaaaaata gcacactatt ttatatcgcc
     3181 gatacaccca aaaatggaaa tagagagaaa taatgtagcg cctataaaca agtta
//
LOCUS       DAOTAX010000124         3180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_24005_length_3180_cov_29.523840, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000124 DAOTAX010000000
VERSION     DAOTAX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24005_length_3180_cov_29.523840"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(944..2080)
                     /gene="nspC"
                     /locus_tag="PLM04_08485"
     CDS             complement(944..2080)
                     /gene="nspC"
                     /locus_tag="PLM04_08485"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HON03143.1"
                     /translation="MIDFSKIPSPCFVLDEHALRRNLELIRYVKEESGAEIILAFKAF
                     AMWSVFPIIKEYGITKSAASSLAEARLAYEEMGNKAHTYSPVFSQKEFSEILSYSSHI
                     TFNSLLQFHQFHSQAKAKGVSCGLRINPEYSEVKTDLYNPCSPGSRLGVTFDLLGKKL
                     PDGIEGLHFHNLCESNSYELEKTLEEVKKKFGKYFSQVQWINMGGGHLMTKEGYDIEH
                     LISIIRSFKKSYSHLKVILEPGSAFAWQTGVLLATVMDIVENHGIKTAMLDASFACHM
                     PDCLEMPYKPAIIGATDEQKGKPTYRMGGNSCLAGDFFGNWSFENELKIGDKIIFEDM
                     MHYTMVKTTMFNGVTHPSIGIWTKENKFKLIKKFGYEDYKSRLS"
     gene            complement(2189..2869)
                     /locus_tag="PLM04_08490"
     CDS             complement(2189..2869)
                     /locus_tag="PLM04_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HON03144.1"
                     /translation="MKKQVSIFFMLCGLLFTVCLITANIVEQKLIALGPIEATAGLLI
                     FPVSYIVNDLIAEVWGYRKARLIIWSGFAMNFLAVVIFRLSMLIPPSHNFQNQEAFAL
                     IMGNTLRITIASFVAFLVGSFLNAFVMSKMKILQHGRHFSVRAIVSTLVGEGCDSLVF
                     FSLAFAGVIPTRDLFLLMLTQTAMKTGYEIVVLPLTNFVVNWMKKKEETDVFDYDISY
                     SPIKLKEI"
BASE COUNT         1071 a          568 c          538 g         1003 t
ORIGIN      
        1 gtacgcgtct ggggggcgtg tggtcgctgg ttcgaatcca gtcatcccga caaaatataa
       61 agtctttgat aatcaaatag taatatattt gtttcaaagg cttttttgta tttttaattc
      121 aatattattg taaaaacaag ttggactttt tcccaaattc taaaagtttt ctccgatttt
      181 tgaccaattt tactgcaaat ctactgcaaa tttataaaca cacttttggc ttctgaaaaa
      241 taatacctcg acaaaaggag tataaaaaaa agggttctta tcctttgaaa ttttccgtta
      301 cgcacggtta gttttttaaa attccgttcg atgtgaacat acaggcagaa aattggatcg
      361 aaaaaagaat taagggggaa tattaaaaat aaaaattttt ttgaacaact attgattaac
      421 tccttataaa ttgggtatct atatattttt aaactgaaaa ttgataaacc ttttgttgaa
      481 aaaataattg aagagccccc ctctgtttct caaagagaat tagtttttaa agacattgag
      541 ataaaaaagg gtaaaaaaat attcaaaaag atttttgaaa atttccatat aatacttcag
      601 taccagttct gacgtatttc gttggaatga gtttgaacga tttcagcttt tcgaacgtaa
      661 gttttttgag tgaagtgggc acagtgttga aaggaacgtt taagcgaaaa caaaatcaaa
      721 tttatttgat ttatgttgag cgtagctact tcaactaagt taattttttt tcttactttt
      781 ttgtttcaag acaaaaaagt aagtgaagtt tggaacaaag cccctttatt aaatatctga
      841 aaataaaaaa tataaatgtt tttaaaattt ttttgtcatg tactaagaca aagaatatat
      901 tttggcagat actgatatgg taagaaactt aggcaatcat attttacgac aaccgactct
      961 tatagtcctc atatccaaat ttttttataa gcttaaattt attttctttc gtccatatcc
     1021 cgatgctggg atgtgtaact ccattaaaca tagtggtttt caccattgta taatgcatca
     1081 tatcttcgaa aataattttg tcgccaattt tcaattcgtt ctcgaatgac caatttccaa
     1141 aaaaatcacc tgccagacaa ctgttccctc ccatacgata ggtaggtttc cccttttgct
     1201 catctgtggc tccaataatt gcaggtttat aaggcatttc caaacaatcg ggcatgtgac
     1261 aggcaaaaga agcatccagc attgcggttt ttattccgtg attttcgaca atatccatta
     1321 ctgtggcaag gagaactcct gtttgccagg caaaggcact tccgggctca agaatcactt
     1381 tcaaatggga atacgatttt ttgaaggaac gaataattga aatcaaatgt tcgatatcgt
     1441 aaccttcttt tgtcatcaaa tgtccaccac ccatattgat ccattgtacc tgcgaaaaat
     1501 atttgccaaa ttttttcttt acttcttcga gcgttttctc caattcatag gaattagatt
     1561 cacataagtt gtgaaaatgc aagccttcta ttccatccgg tagttttttt cccagtaaat
     1621 cgaaagttac tccaagtctt gaccctggcg aacaaggatt gtataaatcg gtttttactt
     1681 cggaatactc cggatttatt cgtaaaccac aagacacacc ttttgcttta gcctgagaat
     1741 gaaattgatg aaactgtagc agggaattaa aagttatatg gctactgtaa gataaaattt
     1801 cggaaaattc tttttgggaa aaaactggag aataagtatg agccttattt cccatttctt
     1861 cgtatgccaa tctggcttct gccagagaac tggcagccga tttagtaata ccgtattcct
     1921 taataatagg gaacacgctc cacatagcaa aagccttaaa agccagtatt atttccgctc
     1981 ccgattcttc cttcacatat cgtattaatt caagatttcg tcgaagtgca tgctcatcca
     2041 aaacaaagca gggagaagga atttttgaaa aatctatcat gcagtgtgat cgttaatttt
     2101 tttacaaagt tacaggatta ttttctattt acatagatac agtttgatgc aaagatggtt
     2161 tgagaacaac ttctacacaa ttttattttt atatttcttt cagcttaatc ggactgtaag
     2221 aaatatcata gtcaaatacg tctgtttcct cctttttctt catccaattg accacaaaat
     2281 tagtaagggg caaaacaaca atttcgtatc ccgttttcat agccgtctga gtaagcatta
     2341 agagaaacaa gtcgcgggtt gggatgacac cggcaaatgc cagcgaaaaa aacaccagcg
     2401 agtcgcatcc ttctcccaca agcgtggaaa cgatagctcg aacagagaaa tgacgaccat
     2461 gttgtaaaat tttcatcttg ctcataacaa aagcattcag aaaagaacca acgagaaaag
     2521 caacaaagct cgcaatcgta atgcgtagcg tatttcccat aatcaaagca aaagcttcct
     2581 gattttgaaa attatgtgaa ggtggaataa gcatgcttaa cctgaaaatg accacagcca
     2641 aaaaattcat ggcaaatccg ctccaaataa tcaaacgtgc tttccgatag ccccaaactt
     2701 cggcaatcaa atcgttcaca atataggaaa cagggaaaat aagcaaaccg gcagtggctt
     2761 cgatagggcc taaggcaatc agtttttgtt caacaatgtt agcggtaata agacagacgg
     2821 taaacagcaa accgcacaac ataaagaaaa tggaaacttg ttttttcata tgtcttgttt
     2881 tttacgtggt gttcaagcac acgttctgca agaaaagcag cagaaacttt ttgcaaaatt
     2941 aacgcttatt tctttaaaaa acaaaagcag gtagtcccca aaagtgtact tttttaaaaa
     3001 tacaatattc aaattgttca gtagagacgc agtatgctac gtctctacaa attaattcat
     3061 aatcaacgag ttataaaaat aaaaaatatt gttcggtatt gcctccaggc tatttcgcag
     3121 ttaaaaataa gctaataagg ttttagatgg gaaggaagtt ttgccttgtt actaaggtta
//
LOCUS       DAOTAX010000125         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_24148_length_3164_cov_36.895143, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000125 DAOTAX010000000
VERSION     DAOTAX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24148_length_3164_cov_36.895143"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..2903
                     /locus_tag="PLM04_08495"
     CDS             6..2903
                     /locus_tag="PLM04_08495"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HON03145.1"
                     /translation="MQSLNNMEFSDGEWTRFVEEYLDKPSDNLVEKTRKIHNDYIYDF
                     VFDDGHIQNIYLVDKKNVTRNKVQVISQFEQKGYHANRYDVTILVNGLPLVQVELKKR
                     GVAIREAFNQVHRYSKESLNSQNSLFKYIQLFVISNGTDTRYFANTIKRDKNSFDFTM
                     NWAKADNMLIKDLKDFTATFFEKRVLLNVLLTYSVFDTSDTLLIMRPYQIAATERMLW
                     KIESSYKAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATQLDFIDKVFFVVDRKDLD
                     YQTMKEYQRFSPDSVNGSESTAGLKRNIEKDDNRIIVTTIQKLNNLMKSESDLAIYQK
                     QVVFIFDECHRSQFGEAQKNLKKKFKKYYQFGFTGTPIFPQNALGAETTASVFGRELH
                     AYVITDAIRDEKVLKFKVDYNDVRPKFKNIESEQDEKKLNAAENKKALLHPERIKEIS
                     QYILQNFRIKTHRTQGSNKGFNAMFAVSSVDAAKCYYDELNDLQKESDKPLKIATIFS
                     FAANEEQSAIGEIADETFEPSAMDVTAKEFLTKAINDYNAMFKTSYSVDSNEFQNYYR
                     DLAKRVKNKEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLIQAFSRTNRIYDATK
                     TFGNIVTFRDLEQATIDAITLFGDKNTKNVVLEKSYKEYLEGFTDIITGEARRGYVEV
                     VKELNEKFPNPDEIVKEKDKKEFVKLFGEYLRIENILQNYDEFNHLKTLQTIDINNPE
                     AVEEFKKTHFISDEDFATMQQIELLKERTVQDYRSTYNDIRDWLRREKSGKEPEKSTI
                     DWDDVVFEVDLLKSQEINLDYILELIFEHNKKNKNKTDLIDEIRRVIRASVGNRAKES
                     LVVDFINETDLDTLQDKANVIESFFMFAQRKQKEDAQALIREENLNEEAAKRYITSSL
                     KREYASENGTELNALLPKMSPLNPQYLTKKQSVFQKLVSFVEKYKGVGGQL"
BASE COUNT         1178 a          522 c          624 g          840 t
ORIGIN      
        1 tgcaattgca atcgcttaac aatatggaat tttcggatgg tgaatggact cgtttcgtgg
       61 aagaatattt ggacaaacca agcgataacc ttgttgagaa aacaagaaaa atccacaacg
      121 actatatcta cgatttcgtt tttgatgatg ggcatattca aaatatctac ttggttgata
      181 aaaaaaatgt cacacgaaat aaagtacagg taatatcaca atttgaacag aaaggttatc
      241 atgccaatcg ctatgatgta accattttag taaatggttt gccattggtt caggttgaat
      301 taaaaaaacg aggtgttgct attcgtgaag catttaatca agttcaccga tacagcaagg
      361 agagtttaaa tagccaaaat tcattattta aatacataca gctttttgtt atttcgaacg
      421 gtacggatac ccgttatttt gcgaacacta taaaacgtga caagaacagc tttgacttta
      481 ccatgaattg ggcaaaagct gacaatatgc tgataaaaga tctaaaagat tttacagcaa
      541 cattttttga aaaaagagta ttgttgaatg tattgctaac ctattcggtt tttgatacaa
      601 gcgatacact tttaattatg agaccgtatc aaattgcagc tacggaaaga atgttgtgga
      661 aaattgaaag ttcatataag gctaaaaact ggtctaagcc agaaagtggt ggttatatat
      721 ggcacacaac aggttccggc aaaacgctta ctagttttaa agcggcacgt ttagccacgc
      781 aattggattt tattgataaa gtattctttg tagttgaccg taaagacttg gactatcaaa
      841 ctatgaagga atatcagcga ttttctcccg atagtgtgaa cggttcagaa agtacagcag
      901 gtttaaagcg aaatatagaa aaagacgata ataggattat agtaacaact atacaaaagt
      961 taaataacct catgaagagt gagagtgatt tagcgattta tcaaaagcaa gttgttttca
     1021 tttttgatga gtgtcatcgt tcgcagtttg gtgaggctca aaagaattta aagaagaaat
     1081 tcaaaaagta ttatcaattt ggttttacag gaacacctat tttcccgcaa aatgctttag
     1141 gtgcagaaac taccgcaagt gtttttggtc gtgaattgca cgcttatgta ataacagatg
     1201 ctattagaga tgaaaaagta ctaaaattca aagttgacta taatgacgtt cgtcctaagt
     1261 ttaaaaacat cgaaagcgaa caagacgaaa aaaaactgaa tgcggcagaa aacaaaaaag
     1321 cattgcttca tccggaacgc atcaaagaaa tatctcagta cattttacag aatttcagaa
     1381 taaaaacgca taggactcag ggaagcaaca aagggtttaa tgcaatgttt gccgtaagca
     1441 gtgtggatgc agccaaatgc tattatgatg aattaaacga cttgcaaaaa gagagcgaca
     1501 aacctttaaa aattgcgaca atcttctctt ttgcagcaaa cgaagagcag agtgcaatag
     1561 gtgaaatagc tgatgagact tttgagcctt cggcaatgga tgttactgcc aaggagtttt
     1621 taactaaagc aatcaatgac tataacgcaa tgttcaaaac aagttatagc gttgatagca
     1681 atgaatttca gaattattat cgcgaccttg ccaaacgtgt aaaaaacaaa gaggttgact
     1741 tactcattgt agtcggtatg ttcttaaccg ggtttgatgc tccaacactc aacactttgt
     1801 ttgttgataa aaatttacgc tatcacgggt taattcaagc cttttcacga acaaacagaa
     1861 tttatgatgc cacaaagacc tttggtaaca ttgtaacatt ccgtgattta gaacaagcaa
     1921 ctatcgatgc tattacactt tttggcgaca agaataccaa gaatgtagtt cttgaaaaga
     1981 gttacaaaga atacctggaa ggctttacag atattattac aggtgaagca cgcagaggtt
     2041 atgtggaagt agtcaaagag ttaaatgaga aatttcctaa ccctgacgaa atcgtaaaag
     2101 agaaagacaa aaaggaattt gtaaaattat tcggggagta tttgagaatt gaaaacatac
     2161 tccaaaacta cgatgagttt aaccatctca aaacactaca aacgattgac atcaacaatc
     2221 cagaagctgt cgaagaattt aaaaagactc atttcatatc ggatgaggac tttgcaacta
     2281 tgcagcaaat tgaattgctc aaagaacgga cagtgcagga ttatcgctct acttacaatg
     2341 atattcgtga ttggttaaga cgagagaaaa gcggtaaaga gccggaaaaa tctaccatcg
     2401 actgggatga tgttgtgttt gaagttgact tgctgaaatc gcaggaaata aaccttgatt
     2461 acattcttga attgattttt gagcataaca agaaaaacaa aaacaaaact gacttaatcg
     2521 atgaaattcg tagggtgata cgtgcaagtg tagggaatag agcaaaagag agtttggttg
     2581 ttgatttcat aaacgaaacc gaccttgaca ctcttcaaga caaagcgaat gttatagagt
     2641 cattttttat gtttgctcaa agaaagcaaa aggaggatgc tcaagcatta atcagagaag
     2701 aaaatctaaa tgaagaagca gcaaaacgtt acataacatc ttctttgaag cgggaatatg
     2761 caagcgaaaa cggaacagaa ctcaatgcac ttttaccgaa aatgagccct ttaaatcctc
     2821 aatacttgac aaaaaaacaa agtgtatttc aaaagttagt atcgtttgta gagaagtata
     2881 aaggagttgg aggtcaatta taaataatga caaagaaaga aaaatgaata gccagtccaa
     2941 cagacacaca cattaccaag ccgcaccagc caacgcacaa accaaagctt ggcaaagagt
     3001 gtgtctgtcc caccctgaca aaattgaatt aaaaaaacct tccctccctt ctgaaaataa
     3061 aaaatacgca accgcacaaa ccgacacgac aaggacggtg aagcgtacta ttgacaatgg
     3121 tttgcaagga cggacgacag aacaccagcc ggtaacatcg gttt
//
LOCUS       DAOTAX010000126         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_24435_length_3131_cov_31.601105, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000126 DAOTAX010000000
VERSION     DAOTAX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24435_length_3131_cov_31.601105"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLM04_08500"
     CDS             <1..632
                     /locus_tag="PLM04_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HON03146.1"
                     /translation="DKTVLGTLAGLFIIVGSILYFFSKATQHQIALYSNFIASFVLIS
                     IIISFILAAMIHKVNVYDAFIEGAKDGFQVAIGIIPYLIAILVAIGMFRAAGAMDMLI
                     RGFTWFFGLIGWNTDFVGALPTALMKPLSGSGARGMMVDAMKLYGADSFVGRLSCIIQ
                     GSTDTTFYILAVYFGSVGIKNTRYAVICGLLADFAGIVAAILIGYLFFH"
     gene            694..2016
                     /locus_tag="PLM04_08505"
     CDS             694..2016
                     /locus_tag="PLM04_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HON03147.1"
                     /translation="MNEIIEYIIKFLLGDNVSDDIVQKIGYTDNEEEFGRYRLVIYSS
                     GFFKPEMYGSIHSIPSLPLKIWDDTPLLYGEPEIEEVGNVRIIHADIIASTYFLISRY
                     EEMVRRGCRDVHGRFYGKESLSYKAGFIDRPLVEEYGKLLRQQLRELGIDIPEPEKKI
                     KKIFLTHDVDQLAHYRRFRGMMGAVARGIKSPKKRAEARRAIKTFFKGIRFDPWYTFP
                     WLFKIDQSLTEAIGQERCKTVVFIKAGGGNKKEDKPNSNLAHPDYKNFIRYCKRKNIV
                     IGLHASYEAGRYPNLIAEEKKKLEHAAKTKITYNRHHFLCSREPEDMEALIEAGLKDD
                     FTLSYADMAGFRLGTCRPVQWINPATRQLRPLILHPFTIMDSTLSDKRYMNMNVFDAY
                     EYCVQLINNVENYGGEFVILWHNTMVEKKSPSYHRKLYKNIIDFLKTK"
     gene            2018..>3131
                     /locus_tag="PLM04_08510"
     CDS             2018..>3131
                     /locus_tag="PLM04_08510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON03148.1"
                     /translation="MQKIAIVCVTNDLVTDQRVHKTCLTLQKCGFFTIEVGRMLPESL
                     PLQRPYVTKRLKLCFRKGPQFYAEYNIRLFFYLLFAKVDLIYANDLDTLPAAFLAAKI
                     RRKKIIYDTHEYFTETPELVNRPKVQAVWEKIEHFIFPKLSDIITVNDSIAELYKKKY
                     KKNLQVVRNIPPTYLPPRLKTRKELGLPEDKHILILQGTGINKDRGAEEIVTAMQYLQ
                     NFILLIIGSGDMLPTLQKMTMELQLEDKVIFKPKMPFEELRQYTCNSDLGLAVDKDTN
                     LNYRFSLPNKLFDYIHAGIPVLASNLPEIKKIINRYDIGYFLENHDPKNIASTIERIF
                     ENDERYKILKKNTEKARKELCWENEENNLLQILQQYS"
BASE COUNT         1006 a          584 c          618 g          923 t
ORIGIN      
        1 tggacaaaac cgttttagga actttggccg gtttatttat tattgtaggc agtattttgt
       61 attttttcag caaagctact caacatcaaa ttgctttgta ttcaaatttc attgccagtt
      121 ttgtactgat ttccataatc atttctttta ttttggcagc catgattcac aaggtaaatg
      181 tgtatgacgc ttttatcgaa ggtgcaaaag acggatttca ggtagcaata ggaatcatac
      241 cttatttaat tgccattttg gttgccattg gtatgtttcg tgctgcggga gctatggata
      301 tgctgattcg tggtttcacg tggtttttcg gactgattgg atggaatacc gattttgttg
      361 gtgccttgcc aaccgctttg atgaaaccgc tgagcggaag tggagcacgc ggtatgatgg
      421 tcgatgccat gaaactatat ggggccgact cgttcgttgg gcggttgtcg tgtatcattc
      481 aaggctcgac cgatacaact ttttacattt tggctgtata ttttggctca gtaggaataa
      541 aaaacactcg atatgccgtt atttgtggac ttttagctga ttttgcaggt atagtagctg
      601 caattttaat cggttatctg ttttttcatt agtttttcta ttagcaactt gatatttgat
      661 aagtgaacag atttttttga aaataatttt tccatgaacg agataattga atatattatt
      721 aaattcctgt tgggcgacaa cgtatcggac gacattgttc agaaaatcgg atataccgac
      781 aatgaagagg aatttggtcg ttatagactg gttatttatt cttccggatt tttcaaaccg
      841 gaaatgtacg gctcaataca ttccattcca tccttgccat taaaaatttg ggacgatact
      901 cctttgcttt atggagaacc ggaaatagaa gaagtaggaa acgtacgtat tattcatgcc
      961 gatataattg caagcaccta ttttcttatt tcgcgttacg aagaaatggt acgtcgtgga
     1021 tgccgcgatg tacacggacg attttacgga aaagaatcgc tttcctataa agccggcttc
     1081 attgaccgac cgttggtgga agaatacgga aaacttttgc gtcaacagtt gcgtgaattg
     1141 ggtattgata ttccggaacc cgagaaaaaa atcaaaaaaa tttttctaac tcacgatgtc
     1201 gaccagctgg ctcattacag acgctttagg ggaatgatgg gagccgtagc tcgaggtata
     1261 aaatcgccaa aaaaacgtgc agaagcacga agagccataa aaacattttt caagggcatc
     1321 cgattcgacc cttggtacac tttcccttgg cttttcaaaa tagatcaatc actgacagaa
     1381 gctatcggcc aagaacgatg caaaacggtt gtatttatca aagcgggagg cggaaataaa
     1441 aaggaagaca aaccaaactc caacctggca caccccgatt acaaaaattt tattcgctac
     1501 tgtaaaagaa aaaatattgt tattggcctt catgcaagtt acgaggcagg gcgataccca
     1561 aatctgattg cagaagaaaa aaagaaactc gaacatgcag caaaaaccaa aattacttac
     1621 aaccgacatc attttctgtg ttcgcgcgaa ccggaggata tggaagcact tatagaggca
     1681 ggcttaaagg atgactttac actatcatat gccgatatgg cgggattcag gctgggaact
     1741 tgtcggcctg ttcaatggat taatccggcc acccgacaac tcagaccgct gatcctccat
     1801 ccttttacca ttatggacag cacattgagt gataagcgct acatgaacat gaatgtattt
     1861 gacgcttacg aatattgcgt tcaattgatt aacaacgtag aaaattacgg cggtgaattt
     1921 gtcattcttt ggcacaatac tatggtagaa aaaaaatcgc cctcctatca tagaaaatta
     1981 tacaaaaaca ttatcgattt tctgaaaaca aagtaagatg caaaaaattg ccattgtatg
     2041 cgtaacaaac gatttggtta ccgatcaacg cgtacacaaa acctgcctga ctttgcagaa
     2101 atgcggtttt tttaccatcg aagttggcag aatgttacct gaaagtttac cattgcaacg
     2161 tccttatgtt acaaaacgac ttaaactttg ctttcgcaaa gggccgcaat tttacgctga
     2221 atataatatc aggctgtttt tttatttact ttttgccaaa gttgacttga tttatgcgaa
     2281 tgatttggat acacttccgg cagctttctt ggcagccaaa attcgcagaa aaaaaataat
     2341 ttacgatact cacgaatatt ttacggaaac acccgaattg gtgaatcggc caaaagttca
     2401 agctgtatgg gaaaaaattg aacatttcat atttccgaaa ctttcggata taatcacagt
     2461 aaacgattcg attgcagaac tttacaaaaa aaaatacaaa aaaaatctgc aagtggttcg
     2521 caatatccct cctacttacc ttcctcctcg attgaaaacc agaaaagaat tgggtttacc
     2581 tgaagacaaa catattttga tacttcaggg aaccggtatc aataaagatc gaggagcaga
     2641 agaaatagtt acagctatgc aatatttaca aaactttata ttgcttatta ttggaagtgg
     2701 agatatgctg ccgactctgc aaaaaatgac aatggaactg caattggaag ataaagttat
     2761 ttttaagccc aaaatgccgt ttgaagaatt acgtcaatat acctgcaaca gcgatttggg
     2821 actggctgtc gataaagata ccaacctgaa ttaccgattt tctttaccaa ataaactttt
     2881 cgactatatt catgccggca ttcccgtgct cgcttccaac ttaccggaaa taaaaaaaat
     2941 tattaaccgt tacgatatcg gctatttttt ggaaaatcac gatccaaaaa acattgcttc
     3001 caccattgaa cgtatttttg aaaatgatga acgctacaaa atactgaaaa aaaatacgga
     3061 aaaagctcga aaagaattgt gctgggaaaa cgaggaaaat aatttgcttc aaattctaca
     3121 gcaatattca t
//
LOCUS       DAOTAX010000127         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_24868_length_3088_cov_31.350478, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000127 DAOTAX010000000
VERSION     DAOTAX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24868_length_3088_cov_31.350478"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..111)
                     /locus_tag="PLM04_08515"
     tRNA            complement(37..111)
                     /locus_tag="PLM04_08515"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Val,seq:tac)
     gene            complement(308..2665)
                     /locus_tag="PLM04_08520"
     CDS             complement(308..2665)
                     /locus_tag="PLM04_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HON03149.1"
                     /translation="MSRIKLRTWLYYVLMLLLFCVGAYLLIQKGSDVEANYHLTTQSV
                     STQVLSPEPNLSNWEQFKVSVSHNMGEPITHLLLQIIAILLVSRLFGLFFSKIDQPIV
                     IAEILAGIVLGPSLLGHFFPNIFNFLFAADSLPNLYILSQIGLILFMFVIGMELNMKA
                     LKEKVSQVLVISHASILIPYFLGMSLAYVYYEEFAVNQTKFLPFALFIGISMSITAFP
                     VLARIVQEKGLTKTHLGTIAIASAAIDDVTAWCLLALVIAISKTGDSVSALYTVGLSV
                     AYILLMFFVIRPFLKKIGEIYQNIETLNKSIVAFLFLVLISSAFITQFIGIHALFGAF
                     LAGVVMPPISNFRKLVIEKVEDVSLTLLLPLFFVFTGLRTEIGLLNSPYLWGICGIFI
                     ATAIVGKFVGGAFSARFLGESWHDSLALGTLMNTRGLMELIVLNIGYEMHILPPSIFV
                     MLVIMAIVTTFMTTPILNLIEKVFPPKNVEEEYIRKQSMGIFKVLIAVGNPENGRMLL
                     RVAKNVLEGVKNTLSVTVLHITPGTDTNPIYGDQFAEESFHKVKQEAEQLNIPIETEY
                     KVVDSIENAIVRMANTNNFDFLLVGAGLSLSGIPFFKERAVFKNIGWLNRLINNISKQ
                     QVVFYPGTLIKDKTKYFIEQSNCTVGVFVNRGFSEISSAAVLLYSEQDEFLLHYARRL
                     LRNNNNASVTIFDLNRVVASSEKVARYVEDLEKQFPASVKLNKQNKINSNSFSKFSLM
                     FISYQTWNALLEKDKSYLSNIPSTLIINKKNSRFHSDGRRRQVLP"
BASE COUNT         1055 a          610 c          539 g          884 t
ORIGIN      
        1 aaaaaaagag ctacatttct gtaactctta gttttgtggg cgttgacgga ttcgaaccgc
       61 cgaccctctg cttgtaaggc agatgctctg aaccagctga gctaaacgcc cttgtttgtt
      121 tatgtacctg ctttcggtaa aagcgttgca aagatacatt gttttttcaa attgacaaaa
      181 aatttgttta atttttcgca tataaaattt ttagatgctg taaattggtt cttattaata
      241 atctcatcat ttaaaatcct gaaaaaatta ataaaactgt ctattcatct acattctctt
      301 ctgaaattta aggtaatacc tggcgtcgtc tgccatcaga atgaaaacgg ctgtttttct
      361 tgttgataat aagtgtagaa ggtatattcg acagatacga tttatccttt tccaacaatg
      421 cgttccatgt ctgatagctg ataaacatca gactaaattt ggagaaactg ttggaattga
      481 ttttattttg cttgttgagc ttgacagaag cggggaattg cttttccaaa tcttcaacat
      541 accgggctac tttctcgctg gaagccacca ctctgttcaa atcgaaaatg gtaaccgagg
      601 cattgttgtt gttgcgcaag agacgtcgtg cataatgtag cagaaactcg tcttgctcac
      661 tgtagagcaa aacagcagct gagctaattt ccgaaaatcc acgattaaca aacactccca
      721 ccgtacaatt actctgttct atgaaatact tggtcttgtc cttaatcaaa gttcccggat
      781 aaaacaccac ctgttgtttt gaaatattat tgatcagtct gttcagccat cctatatttt
      841 taaaaacagc tctttcctta aagaatggaa tacccgacaa ggaaagtccg gctcccacca
      901 gaaggaaatc gaaattgttg gtatttgcca ttcgtacaat ggcattttct atgctgtcaa
      961 ccactttata ttccgtttcg atagggatat tcagttgctc agcttcctgt tttaccttat
     1021 ggaaactttc ctctgcaaac tgatctccat aaatgggatt ggtatcggtg ccgggcgtaa
     1081 tatgaagtac agtaacggaa agagtatttt ttactccttc caaaacattt ttggcaacac
     1141 gtaacaacat tctcccattt tccggatttc ctacagcaat caacaccttg aaaattccca
     1201 ttgactgctt gcgaatgtat tcttcttcca cattctttgg agggaaaact ttctctatca
     1261 aattcagaat cggagtggtc ataaaggttg ttactatggc catgattacc agcattacaa
     1321 aaatggaagg aggaagaatg tgcatttcat aaccaatatt caacacaatc aattccatca
     1381 atccacgcgt attcataagt gtccccaatg ccagactgtc gtgccacgac tcgcccagaa
     1441 aacgtgcgga aaaagcccct ccgacaaatt taccaacaat ggctgtagca ataaaaattc
     1501 cgcaaattcc ccataaatag ggagaattga gcaaccctat ttcggtacgc aaaccggtga
     1561 atacaaaaaa cagaggcaaa agcaaagtca acgaaacatc ttcaactttt tcgatcacca
     1621 attttctgaa attggaaatg ggaggcatta ccacgccggc cagaaaagca ccgaacaaag
     1681 catggatacc gataaattgt gtaataaagg cagaagaaat caaaaccagg aaaagaaaag
     1741 caacaatact tttattgagg gtttcaatat tttgataaat ttcgccaatt tttttcaaaa
     1801 aaggtctgat aacgaaaaac ataagtaaaa tatacgcaac agacaatcca accgtataaa
     1861 gtgcgctaac ggaatcaccg gtttttgaaa tggcaatgac cagtgccagt aaacaccatg
     1921 ctgtaacatc atcaatggct gcactggcta tggcaattgt tcccaaatgt gtcttggtaa
     1981 gacccttttc ctgaacaata cgcgccaaaa cggggaaggc ggtaatactc atggaaattc
     2041 ctataaacag cgcaaaagga agaaatttag tctgattaac ggcaaactct tcataataaa
     2101 cataggccaa cgacattccc aaaaaataag gaatgagaat actggcatga ctgataacca
     2161 gcacttgact taccttttct ttcaacgctt tcatattcaa ctccataccg ataacaaaca
     2221 taaaaagaat caaccctatc tgacttagaa tatacaaatt gggtaaagaa tcggcagcaa
     2281 agagaaaatt gaaaatattg ggaaagaaat gccccaacaa agaaggtccg agcacaatgc
     2341 cggctaagat ttctgctatt acaattggtt gatctatctt ggaaaaaaat agtccaaaaa
     2401 gtcttgaaac caaaagtatg gcaatgattt gaagcaataa atgggtgatg ggctcgccca
     2461 tattgtggga cacagaaact ttaaactgtt cccagttgct gagattgggt tccggagaaa
     2521 gaacttgggt agaaacactt tgtgtggtta gatgataatt tgcctccacg tcgctccctt
     2581 tttgtatcaa caaataagct ccaacacaaa aaagaagtaa catgagtaca taataaagcc
     2641 atgttcgcaa tttgattctg gacatagaat ttactttatc atgctatgca gtgaaaattc
     2701 attttttaaa ttctctgaca gcctcccaca gttaggaaaa gtaaggaaat ccgctacaaa
     2761 aatacaaaaa attaaaaaaa caatgcaaaa ctttctgcta aatcagcacc tttatgttga
     2821 acttagcata gtgttagcta tcgtaaaaga taaatcggtt gtatggcgtg aactatatgt
     2881 attacattaa attattgtac aaataaatta ttatctttgt ccttagtaga tgataaaaaa
     2941 aattgtaaaa acatttatgt tttttatttt cagatattta ataacgaagc tttgccccaa
     3001 acctcactta cttttttgtc ttgaaacaaa aacgtaagca aaaaaattaa cttagttgaa
     3061 gcagctacgc tcaacataaa tcaaataa
//
LOCUS       DAOTAX010000128         3084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_24915_length_3084_cov_19.753714, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000128 DAOTAX010000000
VERSION     DAOTAX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24915_length_3084_cov_19.753714"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..843)
                     /locus_tag="PLM04_08525"
     CDS             complement(202..843)
                     /locus_tag="PLM04_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03150.1"
                     /translation="MFQGLIIQRNAQQIDLAKPLNLPIATIIDLLSKHFDTKYSSEGA
                     SRLPVLALYAAYQCLINETKRFEGKVLLPMESHTSADSRSGRIGDIDIIDEKERAFEA
                     VEVKHGIPITAQLVKDAFEKFKATQVNRYYLLSTANIDTSQADEINKEIERIKNIHGC
                     HVIANGLTNSLKYYLRLLSDTSEFIENYVNLIEIDTALKFEHKTEWNKIISQM"
     gene            complement(758..1303)
                     /locus_tag="PLM04_08530"
     CDS             complement(758..1303)
                     /locus_tag="PLM04_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03151.1"
                     /translation="MEPIEFLEQIYNESMAVVGSDNEIKSDLDTSIANHLLEILGRSE
                     SAKAVITVILTSAVYKRLNPDQDIRNHQTSIPNGYSGRTFDSKFITPFLKSVKFPAMA
                     ESGWLTRSLEQKVPYDSNYSGAIKPDSLKSAFLQTIDFIQQGKDIDKTIKFFVSRTNH
                     PKKRTTNRLSKASKLTHCHYY"
     gene            complement(1305..2525)
                     /gene="dcm"
                     /locus_tag="PLM04_08535"
     CDS             complement(1305..2525)
                     /gene="dcm"
                     /locus_tag="PLM04_08535"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HON03152.1"
                     /translation="MIKYSKLRDKLKVETLNGNAEDLAFFTHYLHNYTNGVSKSFEKR
                     AKEYFETINEELNIVAEPELQAMLPIKWDIPFPAPENPEFTFIDLFAGIGGFRIPLQE
                     LGGKCVFSSEFNYHAQRAYEYNFGEVPFGDITKLNLDIVPKHDILCAGFPCQPFSISG
                     KMKGFEDTRGTLIYHVFELIEKRQPKVVFLENVKHLVYHDNKRTLSTIVQHLEELGYV
                     VSNKVLNASDFGLPQNRERIIIIGHKETKFDFAKIKKKSKPALKDFLDKENEFEYLDE
                     PYTILDDQKMQDSGLVFAGYRNKTIRKAGVRPGTEHLSRVHKQPNRIYSTEGVHPALP
                     SQESSGRFWIWHEGAVRKLTIQECYRIMGFPNKFKLLNNKAELYKQIGNSVAVPMIKA
                     VGEQIKKQLLKIEK"
     gene            complement(2529..2855)
                     /locus_tag="PLM04_08540"
     CDS             complement(2529..2855)
                     /locus_tag="PLM04_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HON03153.1"
                     /translation="MKETFGEYIHKLRADNGLTLTKLAAALDIDQSTLSKIENGKRNV
                     PVDVLPKLLKIFDLDLKQLEHEYFSEKIAEIIYPQEEPSQLLKAAEEKAKYMRIKNLQ
                     QGKINF"
BASE COUNT          831 a          553 c          556 g         1144 t
ORIGIN      
        1 atccatctat gcgatttttt tatgttgtgg ggctcttctg caattgcacc caacgttttg
       61 cggcttggcg tagtggcgga tttttagcac aaatgtttaa ccgaagaact gcactcgaac
      121 ctaccacaaa actgtcatac gaagcacttc acccgccatt acgccaaacc gctgttagcc
      181 gttcgccctt ctattctgtc gtcacatttg agagattatt ttgttccatt ctgtcttatg
      241 ttcaaacttt aatgcggtgt caatttctat aagatttaca tagttctcaa tgaactctga
      301 tgtgtcagaa agaagtcgta aataatactt tagcgaattt gtcaagccgt ttgcaattac
      361 gtggcaaccg tgaatatttt tgattcgctc aatttctttg ttgatttcgt ctgcttgtga
      421 agtatcaata tttgcagtag aaagcaaata atagcggttt acttgagtgg ccttaaattt
      481 ctcaaatgcg tctttaacaa gctgtgctgt tataggaata ccgtgtttaa cttctacggc
      541 ttcaaatgct ctttcttttt cgtctataat gtctatgtct ccaatgcgtc cgcttcggct
      601 gtcagcagaa gtatggcttt ccattggtaa caatactttt ccttcaaatc tttttgtttc
      661 attgattaag cattggtaag ctgcatacaa ggctaaaaca ggaagccttg atgctccttc
      721 tgatgaatat tttgtgtcaa aatgtttact caacaaatca ataatagtgg caatgggtaa
      781 gtttagaggc tttgctaagt ctatttgttg tgcgtttctt tggatgatta gtccttgaaa
      841 caaaaaactt aatagtttta tcaatgtctt tcccttgctg aataaagtca atggtttgca
      901 gaaatgcaga ctttaatgaa tctggtttaa ttgcacctga atagtttgaa tcataaggaa
      961 ctttttgctc taacgaacgt gtaagccaac cgctttctgc cattgcaggg aattttacac
     1021 tctttagaaa tggtgtaata aactttgaat caaatgttct tcctgaatat ccatttggaa
     1081 tactggtttg gtggtttcta atatcttggt caggattaag tcttttgtaa actgcacttg
     1141 tcagaattac tgtaataacg gcttttgctg attcagacct tcctaagatt tcaagaaggt
     1201 ggtttgctat agatgtatct aagtcggatt ttatttcatt gtcagaacca acaactgcca
     1261 ttgattcatt atagatttgt tctaaaaact ctattggttc cattttattt ctcgattttt
     1321 aaaagttgtt ttttgatttg ttcacctacg gctttaatca ttggaactgc aacagaatta
     1381 ccaatttgtt tgtaaagctc tgctttgtta tttaggagtt tgaatttgtt tgggaagccc
     1441 ataattctgt agcactcctg aatagttagt tttctaactg ccccttcgtg ccaaatccaa
     1501 aatctacctg atgattcctg agagggtagt gcaggatgca ctccttctgt tgaataaatt
     1561 ctattcggtt gtttgtgaac tcttgataga tgttctgttc ctggtcttac tcctgctttt
     1621 ctaattgttt tatttctgta acctgcaaat accaaacccg aatcctgcat tttttggtcg
     1681 tccaaaatag tatatggttc gtccaaatat tcaaactcat tctctttatc taaaaagtct
     1741 ttaagtgcag gttttgattt ctttttgatt tttgcaaagt caaatttggt ttccttgtgt
     1801 ccaataataa ttatgcgttc tctgttttgt ggtaaaccaa agtcagatgc atttaatacc
     1861 ttatttgaaa caacatagcc taattcttca agatgctgaa ctattgttga aagtgttctt
     1921 ttattatcat ggtaaactaa gtgcttaaca ttttcgagga atacaacttt aggttgacgt
     1981 ttttcaataa gttcaaaaac gtgataaatc aatgttcccc gtgtatcttc aaagcctttc
     2041 atttttcccg aaatactgaa aggttggcaa ggaaatcctg cacaaagaat atcgtgcttt
     2101 ggaacaatgt ctaaatttag ttttgtaatg tcgccaaatg gaacttcacc aaagttgtat
     2161 tcatatgctc tttgtgcgtg atagttgaac tccgatgaaa aaacacattt accgccaagt
     2221 tcttgtaatg gtattctaaa accgccaatt cctgcgaaaa ggtcaataaa agtaaattca
     2281 gggttctcag gagcaggaaa aggaatgtcc cattttattg gtagcattgc ctgtaattca
     2341 ggctctgcaa caatgtttaa ttcttcatta attgtttcaa agtattcttt tgctcttttt
     2401 tcaaaagatt ttgaaactcc attagtatag ttatgtaggt agtgcgtgaa aaaagctaaa
     2461 tcttctgcat taccatttaa ggtttcaact tttagcttgt cccgaagttt tgaatatttt
     2521 atcatagcct aaaagtttat tttaccttgt tgtagatttt tgattcgcat atactttgcc
     2581 ttttcttcag ccgcttttaa aagctggctt ggttcttctt ggggatagat tatttcagca
     2641 attttctcac tgaaatattc gtgttccaat tgtttaaggt caaggtcaaa aatttttaaa
     2701 agcttcggta aaacgtccac tggcacattt ctttttccgt tttcaatttt ggaaagtgtt
     2761 gattggtcaa tgtccaaagc tgctgcaagt ttggtcagtg tcaaaccgtt gtctgccctt
     2821 agtttgtgaa tgtattctcc gaatgtttct ttcattttct tgaaaatttc gccttgacaa
     2881 ttttggcaaa tttacaaata agtttgattc tttgctgtca aagtctgcaa aatttaagtc
     2941 ggtttatttt tggtgatgtc gtcttttagg gtgacggcta acgttttgca gctacaagaa
     3001 gttggcgatt tttaccacaa atgttgatgc ggagaaccaa actttgatta accacaaatg
     3061 tgtctgcgga gcactgaacc gcca
//
LOCUS       DAOTAX010000129         3048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_25268_length_3048_cov_35.588707, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000129 DAOTAX010000000
VERSION     DAOTAX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25268_length_3048_cov_35.588707"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1150)
                     /locus_tag="PLM04_08545"
     CDS             complement(161..1150)
                     /locus_tag="PLM04_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03154.1"
                     /translation="MKLEEAYSWIDPLYQFASLLVYPMYYIYFRLLTVDRKFSFKKHF
                     VYLLPSTILFVLYSIAVFFTPQEEYKAFLFGEEHHSLNHLSYIEIVSFLIRIVFLIQV
                     ILTVIGNFRLIRKYGHKAAQYYSNMEDSSISKVRLLNYTLIITAISSFILGMLGRGFF
                     ASEITYLAIASVVFSSMLFAIGWLGNKQKSLNPAYETDSITETKQKSEELSLIRQEEL
                     LNKIEYFFNDRKIYLNSSLTILDVARLTGTNRTYVSSLINHHFHQNFSAFVNSYRLKE
                     LEEKILFNPEYPHQILAETCGFGSIDSMKRAINLRYKLSLQEFKSEVLLKKVV"
     gene            complement(1668..1880)
                     /locus_tag="PLM04_08550"
     CDS             complement(1668..1880)
                     /locus_tag="PLM04_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HON03155.1"
                     /translation="MLEKSLKIKGMSCSHCATRVEKALNSLDGVEAKVDLASNSAKLT
                     LAKELSNEEIKKAVENVGYEVTEISE"
     gene            complement(1956..2867)
                     /locus_tag="PLM04_08555"
     CDS             complement(1956..2867)
                     /locus_tag="PLM04_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HON03156.1"
                     /translation="MGITQLVDIDLKEIVPFIDWNFFFLAWRLPGKYEGIENLCNCTS
                     CEVGWLQQFPENNRTKAEEALKLFRDAQQMLQDFLHEKSISIHAIFGFFRAVSQGDDL
                     LISFGDQTYILPTLRQQHASSDGFCYSLADFINTENDYMGAFATTVEGAETMAENFER
                     EDDLYRAIMVKTLSDRLAEAAAEWLHYKVRTEFWGYAPDEPLEIKNILKGKYQGIRPA
                     VGYPSLPDQSIIFELQSLLGLEKIGIRLTENGAMFPNASVCGLYFSHPKSRYFMIGKI
                     NDEQLEDYARRRGKTVQEMKKWLAANI"
BASE COUNT         1008 a          540 c          496 g         1004 t
ORIGIN      
        1 cagacatgtg ggttccatca gacctttaaa aaataaatta tattctgatg aaattgtgaa
       61 aaatttaggt ctcagtaaat tatttcgatt gattgttgag aatttttaaa aactttgatg
      121 cttgagaata gtaaagtatt tttattgttt gtttaacccg ttaaaccact tttttgagga
      181 gaacctctga tttgaattcc tgcaacgaca gcttgtatct aagattaatg gcacgtttca
      241 tagaatcaat cgatccgaaa ccacaagttt ctgccaaaat ctgatgggga tactccggat
      301 taaataaaat tttttcttcc agttccttta agcgataact atttacaaat gcactgaaat
      361 tttggtgaaa atgatgatta atgagtgagg aaacgtacgt tcgattcgtg ccggttaatc
      421 gggctacatc caaaatagta aggctgctgt tcaaatatat ttttctgtcg ttgaagaaat
      481 attcaatttt atttagtaat tcttcctggc ggatcaggga cagttcttcg gatttttgtt
      541 tggtttcagt gatgctgtct gtttcataag caggatttaa agatttttgt ttattcccaa
      601 gccatccgat tgcaaacagc atggacgaaa aaactactga agctatagcc agataagtta
      661 tttcggaggc gaaaaatcca cgacccagca ttcccagaat aaaggaagaa atagcggtga
      721 tgataagcgt ataattcagt aatctgactt tggaaatgct gctgtcttcc atattggaat
      781 aatattgtgc agccttgtgt ccatatttcc taatcaaacg aaaattgcct attaccgtta
      841 atattacctg aatcaaaaaa actatccgta tcaggaaact aacgatttca atatagctta
      901 aatgattcag tgagtgatgt tcttcgccga aaagaaatgc tttgtattct tcttgaggag
      961 taaaaaacac agctatcgaa taaagcacaa acaaaatagt ggatggcagc agataaacaa
     1021 aatgtttttt aaatgaaaat tttctgtcga cggtgagtaa acgaaaatat atgtaataca
     1081 tgggataaac cagcaaagaa gcaaattgat atagaggatc gatccagcta taagcttcct
     1141 ctaatttcat aaagtacaag aaatgagatg aatatacaat aaaacaaaaa aacatgaact
     1201 tccccaaaaa agacttggga gcagaataat tatctccttg agtgtttaaa actatacacc
     1261 aaaaaagagt aacataaacc ggagagagca acaaaataat ttttagcata attttatttc
     1321 atctgaaaca aaattaaact aaagaaatga aataaaaaaa caaatttgct acaaaatttt
     1381 accttttact gataaaattt taccatctgc gtagccaaaa attttcgaat gaactatctt
     1441 tgaataattc ttcaagcttc ttataaaatt aacatgtttt tgacgtcaat cttgattgaa
     1501 taaatctttg cagaatgaaa ttttcacaat caaaactatt caaaatttat ttgtctgttg
     1561 attttctaaa gttttgattt tttatctgtt ttgttttaca ttctcgcagc atagggttgt
     1621 ccgtttttcg ggcaacccta tttttaaaac tttttgacaa gccgaaatta ttccgaaatt
     1681 tctgtcactt cataaccaac attttccaca gcctttttta tttcttcgtt gctcaattct
     1741 ttggcaagtg taagttttgc cgagttggaa gccaaatcaa ctttagcttc aacgccatct
     1801 aaggaattaa gtgccttctc tacgcgagtt gcacaatgag agcaactcat tcctttgatt
     1861 ttcaatgatt tttctaacat gattttatct ccttttcaag aaatttttaa taatgaaatt
     1921 atatttagct attttttgtt gttaatcaat taaatctaaa tatttgcagc cagccatttt
     1981 ttcatttcct gaaccgtttt tccccgcctt cgggcataat cttccagttg ttcatcattt
     2041 attttcccaa tcataaaata tctcgacttt ggatgagaaa aatacaaacc acacactgaa
     2101 gcattgggaa acatggctcc attttctgtc aggcgaattc caattttttc gagtcccaac
     2161 aaagattgta actcaaaaat tatagactga tcgggaagag aaggataacc aactgctggt
     2221 ctgataccct gatattttcc tttcaatatg ttttttatct ccaatggttc gtcgggtgca
     2281 tagccccaaa attcggttct aaccttataa tgcaaccatt cggcggcagc ttctgccagt
     2341 cgatcggaga gcgttttaac cataatggca cgatataaat cgtcttctct ctcaaaattt
     2401 tcggccatcg tttccgcacc ttctacagtc gttgcaaaag ctcccatata atcattttct
     2461 gtatttataa aatcggccaa cgaatagcaa aaaccgtctg atgaagcgtg ttgctggcga
     2521 agtgtaggca aaatatacgt ttggtcgcca aaacttatta ataagtcatc gccttgtgaa
     2581 acagcacgaa agaaaccaaa aatggcatga atggatatcg acttctcatg aagaaaatcc
     2641 tggagcattt gctgagcatc gcggaacaat ttgagtgctt cttcagcttt ggtacgatta
     2701 ttttccggaa attgctgcaa ccaccctact tcacaggagg tacaattgca aagattttcg
     2761 attccttcat atttgccagg taatctccat gctaaaaaga aaaagttcca gtcgataaaa
     2821 ggaacaattt cttttaagtc aatatcaact aattgagtta ttcccatttt ttttggtgct
     2881 gccactaacg tattcatttt ctataaataa atcgaattaa tcatttgact ttaacaacaa
     2941 aagcctcaaa attgttgtaa atttacagaa acaaaaggaa tttttttcaa gaattctttt
     3001 gttcggggaa aatttaatta taagattttt gccagtcggt ttaaaata
//
LOCUS       DAOTAX010000130         2996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_25786_length_2996_cov_24.998980, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000130 DAOTAX010000000
VERSION     DAOTAX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25786_length_2996_cov_24.998980"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="PLM04_08560"
     CDS             <1..1998
                     /locus_tag="PLM04_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HON03157.1"
                     /translation="YLLSADTANTTISLKLFDCYWNMREYGEAEKIMENFPVQNFMQT
                     LPEIRIRLAELQARKGNYSQAASLLDGIAGYEERAAFYLNESAQNYIREDSADWLVSP
                     LSFNTSFREYSPALHDGKLFFTANLPLQDKEKSYLWDGRSFSRLWQIPLQELTVEDST
                     EHFLTGTNKISNVTETKYYAPVYEGSDTRPLWSEVSHKFQTDYVTRTGSPKGSPVFLS
                     EKWKYNVGPASFDSLGNIYFSANSNRFKGKKNTVNILQAKFSNGQLLDAQAIFPDDHY
                     SYLHPAISADGTLLIFSSNRPGGKGAYDLYYVTRQNLTSGWSEIKSFADSINTPGNEV
                     FPSLSKDGYLYFSSDGKAGFGGLDIYRIPLQEALNGTGKTELIGYPVNSSYDDFGWAQ
                     GMDTTRGFFTSDRRGDNDDIFAFRYQPKPKISYITGMVLDKKTRTPMPGATVFLLNEK
                     TGEVLVDKADSTGRYTFVINNMGDYIVKAVEINCKDDCLSMDVSSAKPKYVTFPAPRP
                     LLLELTFKPVWVINNVLYDFDKWHIRDDARPILDSVVTILKTYPIKVELGSHTDCRGS
                     FKYNDLLSQRRAESAVAYIVSQGIDPSRITAKGYGEHKLVNHCADGVDCTEEEHQQNR
                     RTEITVLYNPSPANSIDPTPYHKGDRLRKENFPENFFTGCR"
     gene            2695..>2996
                     /locus_tag="PLM04_08565"
     CDS             2695..>2996
                     /locus_tag="PLM04_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03158.1"
                     /translation="MKTKTRKNLAAIARIFSCRVKFLVFAGLICGFSTMVLADHPLPV
                     YHPPRSYPAPVYRYATPYSVTPESSERSFFSVPDYTQRKPFEETDVSMLSSTTKPMR"
BASE COUNT          905 a          557 c          618 g          916 t
ORIGIN      
        1 tatcttttaa gtgccgatac ggcaaacaca acgatttcgc tgaagctgtt cgactgttat
       61 tggaatatgc gcgaatatgg agaagcggaa aaaattatgg aaaattttcc cgttcaaaat
      121 ttcatgcaga cccttcccga aataagaatt cgcttggctg aattgcaggc acgaaaagga
      181 aattattccc aagctgcatc cttgttggac ggaattgcag ggtatgaaga aagagcggca
      241 ttttatctca atgaatctgc tcaaaactat atcagagagg attccgccga ttggttggtt
      301 tcacctttga gcttcaacac atcattcaga gaatattcgc ccgcattaca cgatggaaaa
      361 ttgtttttta cggcaaatct gccgcttcag gacaaggaga agtcttattt atgggatggc
      421 agaagttttt cgcgactgtg gcaaattccc ttgcaggagc tgactgtgga agattctaca
      481 gagcattttc tgacaggtac caataaaatt tctaatgtta cggaaaccaa atattatgcc
      541 cccgtttatg aaggaagtga tacacggcca ttgtggagcg aagtttcaca caaatttcaa
      601 accgattatg taacacgtac cggctcacct aaaggaagtc ctgttttttt atctgagaaa
      661 tggaaataca atgtcggacc ggcaagtttc gattccttag gtaatattta tttttcagcc
      721 aattccaatc gcttcaaggg taaaaaaaat acagtcaaca ttctgcaagc taaattttcc
      781 aatggtcaat tgttagatgc acaggctatt ttccctgatg atcattattc ctatcttcat
      841 ccggccattt cagcggatgg aactttgttg atttttagca gcaatcgacc gggaggaaaa
      901 ggggcttatg atttatatta tgtaacacgc caaaatttaa cctccggttg gagtgaaata
      961 aaatcatttg ccgattccat taatactccg ggtaacgaag tttttccttc cttgagtaaa
     1021 gatggttatc tttatttcag cagcgacgga aaagctggtt ttggcgggtt ggatatttat
     1081 cgcatccctt tacaggaagc attgaatgga acaggtaaaa ctgaattaat tgggtatccc
     1141 gtcaacagct cttatgacga ttttggttgg gcacaaggaa tggatacaac acgaggtttt
     1201 tttacttccg acagacgagg ggacaatgat gatatttttg cttttcggta tcagcccaaa
     1261 cctaaaattt cctatattac gggtatggta ctggataaga aaacacgcac ccccatgccc
     1321 ggtgctaccg tttttttatt gaatgaaaaa acaggagaag tgttggtgga taaagccgat
     1381 tctacaggaa gatacacttt tgttattaac aacatgggcg actatatagt aaaagcagtt
     1441 gagataaatt gcaaggacga ttgcttgtcg atggatgtgt cgtcagcaaa accgaaatat
     1501 gttactttcc ctgctccgcg tccgttgttg cttgagttga ctttcaaacc ggtgtgggtg
     1561 atcaacaatg tgttgtacga tttcgacaag tggcatattc gcgacgatgc acgtcctatt
     1621 ctcgacagtg tggtaactat cttaaaaact tatcccatca aagtagaatt gggctctcat
     1681 accgattgtc gtgggtcgtt caagtacaat gacctacttt cgcagcgtcg ggccgaatcg
     1741 gcagtagcgt atatcgttag tcagggtatc gacccttccc gtatcactgc taaaggttac
     1801 ggagagcata aacttgtcaa ccattgtgcg gatggagtag attgtacgga agaagagcat
     1861 cagcaaaaca gaagaaccga aatcacagtg ctttacaatc cttcaccggc caacagcatt
     1921 gatcccacac cctaccacaa gggcgacagg ctgcgaaagg aaaatttccc cgaaaatttc
     1981 tttaccggtt gtcggtgaaa agcgtttgta agttatgttt ttaagaagga aacatgaatt
     2041 tgttcatcga ctgtttcggg tgcttgttga cgtgtggtat caaccgattg gaaaaatttg
     2101 aaaaataatt aacatttttc aatcgtttgc ggagaaataa taaaaaattt atatagaatt
     2161 gtgaacaatt tctcgtttta tggattatat ttgtatatat tattatttta atgtgagttg
     2221 gatataagaa tattttataa gtaattataa ataagctaat aaggttgatg aaacggaagt
     2281 attcacatat tactaaggtt agtcgtatct aaataaggtt tttctattca aaaaaatgaa
     2341 ccttactttt ttaaataaaa ccttagtaac gataatcaac aacaagaaga aaccttatta
     2401 tcttattaat tatcaacttt ttatattatt aaaacaggta aatcgttata ccgaactcac
     2461 gttaagtaac atagttcatt gagttgcaga aatgaaattt actatttttg aatgcgtaat
     2521 ctgtgttcat gtcaatatac gaagcaatga tttgcggaaa gggagaaaag cctgatggtc
     2581 aggtactctg aaaattgtcg gaaacattca aatgtcattt ggttttataa aaaaacagaa
     2641 caatttcgtt aagataatgt tatatttgta ttgcattttt aacctatgat ggccatgaaa
     2701 acgaaaacac gaaaaaatct tgctgcaatt gcaagaatat tctcttgcag ggtgaagttc
     2761 cttgtatttg cagggttgat ttgtggtttt tctacaatgg ttttagccga tcatcccttg
     2821 ccggtgtatc atccgccccg ttcttaccct gctccggttt atcggtacgc tactccctat
     2881 tccgttacac cggagtcctc cgaacgctcc tttttttcgg tcccggatta tacgcagcgc
     2941 aagccttttg aagaaacgga tgtttctatg ttgtcttcca ctaccaagcc catgcg
//
LOCUS       DAOTAX010000131         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_27661_length_2828_cov_32.229354, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000131 DAOTAX010000000
VERSION     DAOTAX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27661_length_2828_cov_32.229354"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="argG"
                     /locus_tag="PLM04_08570"
     CDS             complement(<1..1175)
                     /gene="argG"
                     /locus_tag="PLM04_08570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HON03159.1"
                     /translation="MKKKVVLAFSGGLDTSFCVKYLSEEKGFDVYTAVANTGGFSDAD
                     LQNIEKRAYQLGAVKHVVLDVTKDYYEKCIRYMVFGNVLRNGTYPISVSSERTFQAIA
                     IINYAKEIGADYVAHGSTGAGNDQVRFDLTFSVLAPEIKILTPIRDLALSREYEINYL
                     RQHGFEADFKKMEYSINKGLWGTSIGGKETLKSDQTLPEEAYPSKVEKQGEEILTIEF
                     EEGEICGVNGQKNSDKIALINLVEKIGSQYGIGRDMHVGDTIIGIKGRVAFEAAAPML
                     IINAHKLLEKHVLTKWQQYWKEQLGNWYGMFLHEAQYLEPVMRNMEKFLESSQQNVTG
                     TVILKLRPYCFTLVGVDSPFDMMKTDMGEYGEVNKGWTADDVKGFTKILSNQLKIYYT
                     "
     gene            complement(1269..1865)
                     /locus_tag="PLM04_08575"
     CDS             complement(1269..1865)
                     /locus_tag="PLM04_08575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON03160.1"
                     /translation="MENNFSMDEITVQVADESYIKYVDTILKTIEEAAKIRGTGIARR
                     SPEYIEQKMKEGKAIIALHGEEFAGFCYIETWSDKKFVANSGLIVVEKFRGHGLAKRI
                     KQKAFELSRERYPDAKIFGLTTGLAVMKINSELGYKPVTFSELTNDEAFWAGCQSCVN
                     YDILQRTGRKYCLCTGMLFDPAAEQKKSSTPTSEKEEK"
     gene            complement(1936..2418)
                     /locus_tag="PLM04_08580"
     CDS             complement(1936..2418)
                     /locus_tag="PLM04_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HON03161.1"
                     /translation="MKNKRNRLKIISDILRSNVVGSQEELLKLLKEQQCEITQATLSR
                     DLKVLKVAKTPLSNGTYKYVLPSYNKPLPPPSANTLNGSLANGVILSLEFSQQLAVLK
                     TKPGYASAIAWDIDNKASDYILGTIAGDDTILVVPREGVEREVVVEIMNDIFGEPKAV
                     "
BASE COUNT          799 a          604 c          466 g          959 t
ORIGIN      
        1 gtgtaataga ttttcaactg attggaaagt attttggtaa atccctttac atcgtcggct
       61 gtccagcctt tattcacttc gccatattct cccatatcgg ttttcatcat gtcgaaggga
      121 ctgtcaacgc ctaccaaagt aaagcaataa ggccgaagtt tcaaaataac ggttcccgta
      181 acgttttgct gtgaactttc aagaaatttt tccatgttgc gcatgaccgg ttcgagatat
      241 tgggcttcgt gtaaaaacat gccgtaccag tttcccaact gttctttcca gtattgctgc
      301 catttggtca acacatgttt ttcgagcaat ttatgagcat tgataataag catgggtgct
      361 gcagcttcaa aagctacccg tcctttgatg ccgataatgg tatcgcccac atgcatatcg
      421 cgaccaatgc cgtattgaga accaattttt tcaaccaaat tgattaatgc aattttatcg
      481 gaattttttt ggccattcac tccgcaaatt tctccctctt caaattcgat agtgagaatt
      541 tcttcgccct gtttttccac tttgctcgga taagcttctt ccggcagcgt ctgatccgat
      601 ttcagagttt cctttccgcc aatgcttgtg ccccacaatc ctttgttgat ggaatattcc
      661 atttttttga aatcggcttc aaatccgtgt tgtcttaaat aattgatttc gtattcgcga
      721 ctcaaggcaa gatcacgaat aggtgtcaga atttttattt ccggtgccag tacactgaaa
      781 gtcaaatcga aacgaacctg atcgttgccg gctcctgtac tgccatgtgc cacgtaatcg
      841 gctccaattt ctttggcata attaattatg gctatggcct gaaaagtacg ctccgaactc
      901 actgaaattg gataggttcc atttcttaac acattgccga aaaccatgta tcgaatacat
      961 ttttcgtaat aatctttggt tacatcgagc accacatgtt tcactgctcc cagctgataa
     1021 gctctcttct cgatgttttg caaatcggca tcactaaatc cgcctgtatt ggcaacagcg
     1081 gtatatacat caaatccttt ttcttccgaa agatacttta cgcaaaacga agtatccaat
     1141 ccaccactaa aggctaaaac aacttttttt ttcatttttc tttgaatatc ttattttttt
     1201 attgattttc tgattttttt cttttttaaa cgggatttaa aacccattta attcgatttt
     1261 aaaagggatt atttttcttc tttttccgat gtcggggtcg aagatttttt ttgttcagca
     1321 gcagggtcaa acaacatgcc ggtacaaagg caatattttc gtccggttcg ttgcagaata
     1381 tcatagttta cacaactttg gcacccggcc caaaaagctt catcattggt aagttcagag
     1441 aaagttaccg gtttgtaacc caattcggaa tttattttca tcacagccaa tcctgtagta
     1501 agaccaaaaa tcttggcgtc aggataacgt tcgcgcgaaa gttcaaaagc tttctgctta
     1561 attcttttgg ccaatccatg tcctctgaat ttttctacca caatcaaacc ggaattggct
     1621 acaaattttt tgtcgctcca agtttcaatg tagcaaaaac cggcaaactc ttctccatga
     1681 agagcaataa tggcttttcc ttccttcatt ttttgctcaa tatattcggg cgaacgacgt
     1741 gcaatgcctg ttcctctgat ttttgctgct tcttcgatgg ttttcaatat ggtatccaca
     1801 tatttgatgt aactttcatc ggcaacctga acggtaatct catccatgct aaagttattt
     1861 tccataattt ttttggattc ctcctatctt cttcatttat aaatttttat ctacgaattt
     1921 catctatcaa aaaaatcaaa ctgcttttgg ctcgccaaaa atatcgttca taatctctac
     1981 gactacttcc cgctccactc cttcacgcgg cactaccaaa atggtatcgt cgccggctat
     2041 ggttcccaaa atgtaatccg atgctttgtt gtcaatatcc caagctatcg cgctggcata
     2101 tcccggtttc gtcttcaata cggccagttg ctgcgaaaat tccaaactta gaatcactcc
     2161 attggccaac gaaccattca gcgtattggc cgaaggtggt ggcaggggct tgttgtacga
     2221 aggcaacacg tatttgtatg tcccattgga aagcggagtt ttggccactt tcaacacttt
     2281 caggtcgcgc gacagagttg cttgcgtaat ttcgcactgc tgttccttca gtaatttcaa
     2341 caattcttcc tggctgccaa ccacatttga acgaagaatg tcggaaataa tttttaagcg
     2401 gtttcgtttg tttttcataa aattttaata tataagtatt tgtttgtaat tacttttctg
     2461 atgaaaccca catgtatttc atacacaaac acaaatgaat ataatattca gacatgtggg
     2521 ttccatcaga cctttaaaaa ataaattata ttctgatgaa aacgaatgtc ttatatgaaa
     2581 ccaaactgat tctctactca aatattttta gtatatttat aagaatattt tgcaaatata
     2641 cgaaatttat tccaaaatca cgaataaata actaataaat atccttatta atgccattat
     2701 ttaacaatta ttcgattaaa caggtatatt tatgcaggaa aaatctaaat aattactcaa
     2761 aacataaaag aatattaaat gaataaaaag tgggtaacac tatcagtatt atgttttttg
     2821 aagtatta
//
LOCUS       DAOTAX010000132         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_28240_length_2782_cov_34.261459, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000132 DAOTAX010000000
VERSION     DAOTAX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28240_length_2782_cov_34.261459"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..1800)
                     /gene="mnmE"
                     /locus_tag="PLM04_08585"
     CDS             complement(403..1800)
                     /gene="mnmE"
                     /locus_tag="PLM04_08585"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HON03162.1"
                     /translation="MNEASTICAISTPPGTGGIAVIRVSGQNAFSCVNKFFVPRKKDT
                     DIFQQSPYTLNLGCFMNARKETIDDVLIGIFRAPHSYTGEDVVEISCHGSVYIQQQIL
                     QTLIDAGCSLAKPGEFTQRAFLNKKMDLSQAEAVADLIAADSAASHRMALNQMRGGIS
                     EELKNLRTRLLDFVSLTELELDFSEEDVEFADRSQLMALANEIEELILKLSNSFKVGN
                     ALKNGIPVAIVGETNVGKSTLLNVLLNEDKAIVSDIHGTTRDAIEDSVNIDGITFRFI
                     DTAGIRETKDEIETLGIERTYQKIEQASIILWLIDCTQLSERIETLTEKIAKKIQDKK
                     FILIFNKIDKITDEEREVLNQFFEHYSGERIYLSAKKRINIHQLQKLLVKAAQLPEVQ
                     PGDVVVSNVRHYEALQNALKAIQRVKEGIQNGISGDFLSQDLRECMFFLGEITGEISS
                     DEILGNIFSKFCIGK"
     gene            complement(1819..2691)
                     /locus_tag="PLM04_08590"
     CDS             complement(1819..2691)
                     /locus_tag="PLM04_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HON03163.1"
                     /translation="MMKYFAPSELLINDDGSIFHLHLKPEHLADKIILVGDPGRVEKV
                     AAHFDSKECSVSNREFNCITGTYQNKRITVVSTGIGTDNIDIVMNELDALANIDLKNR
                     TEKTDFRQLTIVRIGTSGGMQPEIPLGSFLISEKSIGIDGLLSFYARRDEVCDLDFEK
                     AFKKHTKWNPQLASPYVVDNDMELIERIGKDDMLKGVTISANGFYGPQGRVLRLDLHD
                     SHLNDKIESFRYKDYKITNFEMESSAIAGLSKLMGHKAMTVCCIIANRRVESANTNYQ
                     PHIEKLIQTVLDRI"
BASE COUNT          855 a          528 c          476 g          923 t
ORIGIN      
        1 agcatgctac gtctctggaa acagccgtta tcaaatgctc gtatcgtaaa ttttttcggc
       61 ttgaaaagta ggagagaggt tatttaatat gaaacccaca tgtattacat acacaaacac
      121 gaataaatat aatattcaga catgtgggtt ccatcagacc tttaaaaaat aaattatatt
      181 ctgatgaaaa ttgtaccaaa gtgtaaactt tgtaaaccat ttgaggtaca aaatcagtga
      241 aaaaatacct tttatagttc gaggtacaaa taaggtacaa gtatatgata aaaattcaaa
      301 taaaaaacaa tcaacaaaaa taaatttact tttactaaaa tgctaattat atgcatttta
      361 atatgttttt tgtttgtttt gttatttgtt tgtttagggg ggttactttc cgatacaaaa
      421 cttactgaaa atattgccca aaatttcgtc actggagatt tcgccggtaa tttctccaag
      481 aaaaaacata cattcgcgta aatcttgtga aagaaaatcg ccggaaattc cgttttgaat
      541 gccttctttc actcgctgaa tggcttttaa agcattttgc agtgcttcgt aatgtctgac
      601 gttactgacc accacatcgc cgggttgtac ctcgggcaac tgggcagctt ttaccagtaa
      661 tttttgtaat tgatgaatgt tgattctttt ttttgcggaa agataaatgc gttcgcccga
      721 ataatgttca aaaaattgat ttaaaacttc acgttcttcg tctgtaattt tgtctatttt
      781 attgaaaatc agtataaatt ttttatcttg aatttttttt gcaatttttt ctgtaagcgt
      841 ttcgatgcgt tcactaagtt gagtgcaatc aattaaccat agaataatcg aagcttgttc
      901 aattttttga tacgttcgtt caatgccgag tgtttcaatt tcatctttgg tttcacgtat
      961 cccggctgta tcaataaagc gaaaagttat tccgtctata ttcaccgaat cttcgatggc
     1021 atctcgtgtt gttccgtgaa tatcggaaac aatagccttg tcttcattga gcaaaacatt
     1081 tagcagcgta gatttgccta catttgtttc gccaacaatg gctaccggaa tgccgttttt
     1141 tagagcattt ccaactttga acgaattaga aagttttaga attaattctt ctatttcatt
     1201 ggcaagtgcc attaattgcg aacgatcggc aaattctaca tcttcttccg aaaaatcgag
     1261 ctcaagctca gtcaatgaaa caaaatcgag caaccgtgta cgtaaatttt tcaattcctc
     1321 cgaaatacca cctctcattt ggttcaatgc cattcggtgc gaggccgccg aatcggcagc
     1381 aatcaaatcg gcaaccgctt cagcttgcga caaatccatt tttttattca aaaaagctct
     1441 ttgcgtaaat tctcccggct tggctaatga acagcctgcg tcgattaatg tctgtaaaat
     1501 ttgctgttgt atgtacacag aaccatgaca cgatatttcc acaacatctt caccggtgta
     1561 ggagtgaggt gctcgaaaaa ttccaatcaa cacatcgtca attgtctctt ttcgagcatt
     1621 cataaaacat cccagattga gcgtataggg cgattgttga aaaatatcag tatctttttt
     1681 tcttggaacg aaaaatttat tgacacaaga aaaagcattt tgacccgaca cacggatgac
     1741 agcaatgcct cctgtgccgg gtggagtaga aattgcacaa attgtagatg cttcgttcat
     1801 taagaaataa aaatttcgtt aaattctgtc taaaacagtc tgaatgagtt tttcaatatg
     1861 tggctgataa tttgtattgg ctgattctac cctgcgattg gcaataatgc agcacaccgt
     1921 catggcctta tgtcccatga gtttggagag accggcaatt gcagaacttt ccatttcgaa
     1981 attggtaatt ttataatctt tgtatcggaa actttcaatt ttatcattca gatgactgtc
     2041 gtgcaaatcg agacggagta ctcttccctg tggcccataa aatccattgg cagaaatggt
     2101 tacacctttt aacatgtcat ctttccctat tcgttcgatt aattccatat cattatctac
     2161 aacgtaggga gaagccaatt gcggattcca tttcgtatgt tttttaaaag ccttttcaaa
     2221 atccaaatca caaacttcgt ctctgcgggc ataaaaactc aataaaccat ctattccgat
     2281 ggatttttca gaaataagaa aacttcccaa aggaatttcg ggttgcattc cgccggaagt
     2341 tccaatccga acaatggtga gttgtctgaa atctgttttt tcggttctat ttttcaaatc
     2401 aatgttggcc aaagcatcca gctcattcat gacaatgtca atgttatccg ttccaatgcc
     2461 ggtagaaaca accgtaatac gcttattttg ataagtacct gtgatacagt taaattctct
     2521 gtttgaaact gaacattctt tcgagtcaaa atgtgcagca actttttcta cacgtcccgg
     2581 gtctccaacc aaaataattt tatcggccaa atgttcgggt ttcagatgca gatgaaaaat
     2641 acttccatca tcattgataa gaagttcaga aggagcaaag tatttcataa tatttctgtt
     2701 ttaaaaaatt aattcgtttt attgttgcag aagcaattcc aaaggcaaat ataacttatt
     2761 tttaattcaa aactcatttt tt
//
LOCUS       DAOTAX010000133         2747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_28672_length_2747_cov_23.199108, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000133 DAOTAX010000000
VERSION     DAOTAX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28672_length_2747_cov_23.199108"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PLM04_08595"
     CDS             <1..1829
                     /locus_tag="PLM04_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HON03164.1"
                     /translation="GTFKSINTNATKNPVALLEQKRDVSKANDFIGNAEVDYKFFFLP
                     ELKAHLSLGIESSYGKQDLHIDSTATSDYPWGRTGWDKIEKTNRLLNFYFQYAKEINK
                     HSFDIMGGYEWQHYYREGQNEYRSLEKIDSNNDGDIDENDGYYNHITPDQSLWKTENY
                     LVSFFGRLNYSLAGKYLLTATLRDDGSSRFAPENRWALFPSVALAWKISEEHFISERG
                     TFSDLKLRLGYGITGQQDISQGDYPYIPVYTISKEHALYPFGNEYYYTSRPDAYNKLL
                     KWEQTTTWNAGLDAGIFNNRLTGSLDYYFRKTNDLINVVSVPAGTNFRNKVISNIGSL
                     ENKGIEFMISGKPISNKNCTWDISYNVTYNKNRITKLTTGNNEGYFVATGGIFQGSTQ
                     AHTVGYPSNTFYVYQQIYDENGKPIEAGAPKPDGSGDVYKDIEAFVDRDGNNTITEDD
                     KYFYKSAHPDYTMGLSSKFIYKSFDIGFSMHASIGNYVYNAVAAERLNVGDNGIYSLG
                     YLSNKPKSALKTNFQGTSPITYLSDYFVENGSFLRCDNITIGYSFNNLFKAISSLRIY
                     AAVQNPFLITGYSGLDPEIFGGIDNNIYPRPMTTLLGLSVNF"
     gene            1843..>2747
                     /locus_tag="PLM04_08600"
     CDS             1843..>2747
                     /locus_tag="PLM04_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HON03165.1"
                     /translation="MNNKYLNIFGVLLVAFTFHACINDLDVTPINPQVTQTFNQDQVF
                     AKVYAAYALTGQEGPAGNNDIDIVDEGRFSLFRCLWNCNELSTDEAACAWGDAEVIDL
                     NQNTWTAGNFSTEALYARLYFIVSISNHFLEETAKITDEKTLKQRAEVKFLRALAYYY
                     LMDNFGNVPFTTIVGTEAPHQIKRADLFAWIRGELKAIENDMYEPRQAPYYRVDKAAN
                     WLLQARLYLNSEVYTGTAKWDSAAIYAKKVIDSGYQLAPTYRHLFMGDNAGTLDGSNV
                     NCAPQEIIFPLAADGIKTQSWGLSL"
BASE COUNT          892 a          517 c          549 g          789 t
ORIGIN      
        1 atggtacatt caaatccatc aataccaatg caacaaaaaa tccggtggca ttacttgaac
       61 aaaaaagaga tgtttccaaa gcaaatgact ttattgggaa tgcagaagta gattacaaat
      121 tcttcttttt gcccgaatta aaagctcact tgagtctggg tatagaaagt tcttatggca
      181 agcaggattt gcatatcgat tccacagcta caagcgatta tccgtggggt cgtacaggtt
      241 gggacaaaat tgaaaaaacc aatcgtttgt taaattttta tttccaatat gccaaagaaa
      301 taaacaaaca tagttttgat attatgggtg gctacgaatg gcaacattat tatagggaag
      361 gacaaaacga gtatcgaagc ttggaaaaaa ttgacagcaa caacgatgga gatatagatg
      421 aaaatgatgg ttattataac catattacac cggatcaaag tctttggaaa acagaaaatt
      481 atttggtatc cttctttggt cgattgaatt attcccttgc cgggaagtac ttgcttacgg
      541 caactttacg cgatgacggt tcttcaagat ttgcgcccga aaatcgctgg gctttattcc
      601 catcggttgc tttggcatgg aaaattagcg aagaacattt tattagtgag cgaggtactt
      661 tttctgacct gaaactcaga ttgggttatg gaatcacagg tcaacaggat atcagtcagg
      721 gcgattatcc atatattccg gtttatacca tcagtaaaga acatgcttta tatccgtttg
      781 ggaatgagta ttattatact tcccgtcctg atgcatacaa caaactccta aaatgggaac
      841 aaaccactac ttggaatgcc ggtttggatg caggcatatt taataaccgc ctcactgggt
      901 cgttagatta ctatttcagg aaaaccaatg atttgatcaa tgtagtttct gtacccgccg
      961 gaaccaattt cagaaataaa gttatcagta atataggatc ccttgaaaat aaaggaatcg
     1021 aatttatgat cagtggcaaa cctatctcca ataaaaactg tacgtgggat ataagttata
     1081 atgtaaccta caataaaaac agaattacaa aattgacaac tggcaacaat gaaggatact
     1141 ttgtagctac aggaggaatt ttccaaggct ccacgcaggc tcatactgta ggttatcctt
     1201 ccaatacttt ttatgtgtac cagcaaattt atgatgagaa cgggaaacct atagaagcag
     1261 gtgctccgaa acctgatgga agcggagacg tttacaaaga tatcgaagct tttgttgaca
     1321 gagatggaaa caacacaatc acggaagatg acaagtattt ttacaaaagc gctcatcccg
     1381 attacacgat gggactttct tccaaattta tttacaagtc atttgacatt ggattctcaa
     1441 tgcatgcgag cataggcaat tatgtttaca atgctgtggc tgcagaaaga ctaaacgtgg
     1501 gagacaacgg catttattca ttgggttatc tttccaacaa acccaaatcg gcattgaaaa
     1561 caaactttca gggaacttct cccatcacct atttgtcgga ttattttgtg gagaacggtt
     1621 cttttttgcg ttgcgacaac ataaccattg gatatagttt caacaatctg tttaaagcca
     1681 tctcaagcct aaggatttat gctgctgtac aaaatccttt cctgatcacg ggttattccg
     1741 gtctcgatcc cgaaatcttt ggcggcatcg acaataatat ttatccgcgt cccatgacaa
     1801 cattactggg tttatctgtt aacttttaaa aaaatgaaaa acatgaataa taaatattta
     1861 aacatttttg gcgtgttatt ggtggcattt acttttcatg cctgcataaa cgatttggat
     1921 gtaactccca ttaatcctca ggttactcaa acctttaatc aggatcaagt gtttgcaaaa
     1981 gtatatgccg cttatgccct gacaggacag gaaggacctg caggcaacaa tgacatagat
     2041 attgtggatg aaggaagatt ttctcttttc cgctgcttgt ggaattgcaa tgaactctcc
     2101 acagatgaag ctgcttgtgc ttggggagac gccgaagtaa tcgatttaaa ccaaaacaca
     2161 tggacagccg gtaatttctc aaccgaagca ctgtatgccc gtttatattt tattgtttcc
     2221 atcagcaacc attttttgga ggaaacagca aaaattaccg atgaaaaaac cctgaaacaa
     2281 agagcagaag tgaaatttct cagagcactg gcttattact atttaatgga taattttggg
     2341 aatgtgccat ttactactat tgtaggaaca gaagcgcctc atcaaattaa aagagccgat
     2401 ttgtttgcat ggattagagg ggagctgaaa gccatcgaaa acgatatgta cgaaccgcgc
     2461 caggctccat attatcgagt agataaagct gccaactggc ttctacaggc aagattatat
     2521 ttaaactcag aagtatatac aggaacagct aaatgggact ctgctgccat ttatgcaaaa
     2581 aaagttattg attcgggata ccaattagca cctacttatc gacatctctt tatgggagat
     2641 aatgccggaa cattggatgg ctcgaacgtg aactgtgctc ctcaggaaat aatttttcct
     2701 ctcgcagccg acggaatcaa aacacaaagt tggggattat ctttatt
//
LOCUS       DAOTAX010000134         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_28943_length_2726_cov_31.320854, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000134 DAOTAX010000000
VERSION     DAOTAX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28943_length_2726_cov_31.320854"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1216)
                     /gene="prmA"
                     /locus_tag="PLM04_08605"
     CDS             complement(377..1216)
                     /gene="prmA"
                     /locus_tag="PLM04_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HON03166.1"
                     /translation="MDYLEVNFTLSPYEEFIADLLMETLGEIGFESFQLADQQFFAYI
                     QESNFNQKILDDKLSKFEYPVSIDYNILLLENKDWNEEWEKNFFQPILIDNDCVVHSS
                     FHQKVPEAKYDIVINPKMAFGTGHHETTAMMLGELLKMNLGKKNLLDMGCGTAVLSIL
                     ASLRGASSVVAVDIDDWCIENSMENIRLNKIKNIELRKGGAEVLKDVHVDIVLANINR
                     NILLKDMKFYAACLPTGGELYLSGFYVEDIPMIEAEAEKNGLQLAYFKEKNNWALVKT
                     IKK"
     gene            1447..>2726
                     /locus_tag="PLM04_08610"
     CDS             1447..>2726
                     /locus_tag="PLM04_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03167.1"
                     /translation="MKPAKLCILFLSVLLALPGYGQKNKASIDDVYFSWDDAKNTAKM
                     STESNKKPNTKNGAKEIIFIDRNEDAQNNSSRLLIAKANDSTTVDSDTLAADEGYYLR
                     DFNGSESDYEYAERIRRFHNPKFTIHISDPEYMDIYFLDDSQWNVYVGGSYAWITPTW
                     TNPAWFDYYYRPFSYNSWYWRSSLYYPYSFYGYHSWAYSPWYYDPWYYDPWFYYPAYY
                     RPYYGYPYYGYYGWSRPFYKSVDPSHNEAYRRQVANGSRNSNTNSSTRAGILSGVAGG
                     SSSTGRSGNPYTVVSGSGSRSSVSSDINSTGRRSSTSSYDNSRSSINSGRTTTGTRSQ
                     SEVTGTTGTRSTETYSGNSSRRTTETINTRPRTSSGNSGNSVATPQTGSRSSYTPSSG
                     SSERRSSYTPSSSDNSSRSSGSSYSGSSRSSGSSS"
BASE COUNT          864 a          545 c          475 g          842 t
ORIGIN      
        1 gaaacccacc tgtattgcat acacaaacac aaatacagac atgtgggttc catcagacct
       61 ttaaaaaata aattatattc tgatgaaagt taagaaagat aaggcttgat agccgagaaa
      121 tgaaaggcaa aaattcattt tagagcgaag atataaaggt ttgaatatag ttaaaacaat
      181 tattgctccg agaatcagac tgcccaaaag tgataaaaca ttgatcgaga tatgcaaatt
      241 actctaaaaa aattaatcgt tacccatttt tgatgatatg ttgtggaaaa tttttagggt
      301 gaagcattgt tgaagtcagg aatttatatt ctcaagcttt caatattaat aaatattttt
      361 atgtgtaaat agatgtttac ttttttattg tttttaccaa tgcccagttg tttttctctt
      421 tgaaatacgc caactgcaat ccattttttt cggcttctgc ctcaatcata ggaatatctt
      481 ccacataaaa tccgcttaga tagagttctc ctcctgtcgg caaacaagct gcgtaaaatt
      541 tcatatcttt cagcaaaata tttctgttta tgttggcaag cactatatca acatgtacat
      601 ccttaagcac ttcggctcca ccttttctga gttcaatgtt ttttatttta ttcaatcgaa
      661 tgttttccat tgaattttca atgcaccagt cgtcaatgtc aaccgctacc actgatgaag
      721 ccccgcgcaa ggatgccaga atggacaata ccgccgttcc acaacccatg tccaaaagat
      781 ttttctttcc cagattcatt ttcaacaact cacccagcat cattgcggtc gtttcatgat
      841 gacccgtacc aaaagccatt tttggattga taacgatatc gtattttgct tcgggaacct
      901 tctggtgaaa agaactgtga accacacaat cgttatcaat caatatcggc tgaaaaaaat
      961 ttttttccca ttcttcgttc cagtccttat tttccaacaa taatatattg taatcaatgg
     1021 aaacaggata ttcaaatttg gacaacttat catcgagtat tttctggttg aaattgcttt
     1081 cttgaatata agcaaaaaac tgttggtcag caagttgaaa actttcaaat cctatttcac
     1141 ccaatgtctc catcaaaaga tcggcaataa actcttcata tggagacaat gtgaaattca
     1201 cttcaagata atccataata tttgacgcgt taaaatcgct aagaaaccat aaacgaatta
     1261 aaccttttca aaaaaagtaa atctaaaata taaaactcaa caaaattaca tatttttgtt
     1321 gagttcttca attgactaac tttttagtaa aaccgtaaaa ataagtatgc ttttttctta
     1381 caatggtaca attattgatg cttatataat tgcaaaatct atctacaaaa cacaggaggt
     1441 aatatcatga aacccgcaaa attatgtatt ttatttttat ctgttctcct tgccttgccg
     1501 ggatatggac agaaaaacaa agcatcgatc gacgatgtgt atttttcgtg ggatgatgca
     1561 aaaaataccg ccaagatgag tactgagtcc aataaaaagc ccaataccaa aaatggagca
     1621 aaagaaatta tttttatcga ccgaaatgaa gatgcacaga ataattcttc acgattattg
     1681 attgctaaag ccaacgacag tacaacagtt gactctgata cacttgctgc agatgaaggt
     1741 tattatttga gagattttaa tggaagcgaa tcggattacg aatatgctga aagaattcgc
     1801 cgttttcaca acccgaaatt taccattcat atttctgacc ccgaatacat ggatatttat
     1861 tttttggacg atagtcaatg gaatgtttat gtaggcgggt cttacgcatg gattactcct
     1921 acatggacca atcctgcatg gtttgattat tactatcgtc cgttcagtta taatagctgg
     1981 tattggcgaa gcagtcttta ttatccgtat tctttttacg gataccattc gtgggcctat
     2041 tcaccatggt attatgatcc atggtattac gatccatggt tttattatcc cgcttattac
     2101 aggccttatt acggttatcc ttattatgga tattacggct ggagtcgacc tttttacaaa
     2161 tctgttgatc caagtcataa tgaagcctat cgccgtcagg ttgctaatgg ttcgagaaat
     2221 tcgaatacca atagttctac aagagcaggc attctctccg gcgtagccgg cggaagctct
     2281 tcgactggcc gaagtggtaa tccttatacg gttgtaagtg gtagcggttc cagaagttct
     2341 gtttcgtccg acataaattc gactggccgc agaagttcaa cttcaagtta tgataattca
     2401 agatcttcta tcaattcggg cagaaccaca accggaacca gaagtcagag tgaagttacc
     2461 ggtacaacag gaactcgttc gacggaaact tatagtggaa attcttcgcg cagaaccaca
     2521 gaaacaatta atacccgacc gagaaccagc tccggaaata gcggaaattc ggttgcaacg
     2581 cctcaaaccg gttcacgaag ttcttacact ccatcttccg gttcttcaga aagaagatct
     2641 tcttacacgc ccagcagttc cgacaatagt tcgcgaagtt ccggcagttc ctattccggt
     2701 agctcgcgca gttcgggcag cagttc
//
LOCUS       DAOTAX010000135         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_30293_length_2628_cov_35.407307, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000135 DAOTAX010000000
VERSION     DAOTAX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30293_length_2628_cov_35.407307"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1817
                     /locus_tag="PLM04_08615"
     CDS             354..1817
                     /locus_tag="PLM04_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HON03168.1"
                     /translation="MNSNDIRQLQPQQLWQNFYSLTQIPRPSGKKEVISQFLENFGKS
                     LGLETLRDEIGNVLIRKPATSGMEKRKPVILQAHMDMVPQKNSNVSHNFDTDPIQAVV
                     DGDWVRAQETTLGADNGIGLAAALTVLQSDNIAHPALEVLVTVDEETGMHGAFALKPN
                     FLKGEILLNLDSEDEGELYIGCAGGLDADITFRYQEVPVPESDVALKISLTGLKGGHS
                     GVDIHLQRANANKLLFRFLKKAVAEYEARLAWVEGGNLRNAIPREAFALITIPADGVD
                     DLIELLKDFENLFVEENQFVEDNIRLQVEKVKLPTGLMPEEIQDDLINGVTACPNGVF
                     RFIPEVFDVVETSNNLAVVKSDGSTIRLRCLLRSSVESRKEELASMVESVFSLAGAHI
                     DFSGGYPGWKPNLDSPILKVMTEVYEKQFGSKPKIKIIHAGLECGIIGMHYPDMDMIS
                     FGPTIRYPHSPDEKVNIASVEKFWDFLLATLQNIPEK"
     gene            1950..>2628
                     /locus_tag="PLM04_08620"
     CDS             1950..>2628
                     /locus_tag="PLM04_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HON03169.1"
                     /translation="MMKKLTIISILILSILQLYAQKDTTAVDSFRFTTIKENKITPIK
                     DQASSGTCWSFSGLAFIESELLRMGKPETDLSEMFIVHHTYSDKADKYVRMNGTINFA
                     AGGSFFDDLYALKNYGIVPEAIMNGLNYGEEKHRHGELDALTEAYVKVIVTNPNKKLS
                     TAWKQGFDGILDAYLGKCPEKFIWEGREYTPLTYFQSLGINPDDYVSLTSFTHHPFYT
                     RFAIEIPD"
BASE COUNT          823 a          509 c          550 g          746 t
ORIGIN      
        1 tttgatcagc ttgtgggatg tttatgaatt gatctgggaa caaaaagacc tttaaaaaag
       61 aatcaggagg ctacaccttt cacagatcag ttaaccttga acccgcaatt ttgaggtgaa
      121 gtctcttttg aaaaaacaat atgctttatt cagaagggtt acaagtataa atatcttcta
      181 aatgaaagtt tttctgataa tggtgatttt caacttcttt ttttaccaat ctgtagaata
      241 ttttgtaaaa aatcttttca actgttcatt tatctgaaaa atttgagcat atttgtattc
      301 taaaaattgg aataaatatt cagaatttac aaaactctaa taataaacat attatgaata
      361 gcaatgatat tcgtcagctt caaccccagc aattgtggca aaacttttac agtctgacac
      421 agattccgcg tccttcaggg aaaaaagaag ttattagtca atttctcgaa aattttggaa
      481 aatcactcgg gttggaaaca ttgcgggatg aaatcggcaa tgtattgatt cggaaaccgg
      541 cgacttccgg catggaaaaa agaaaacccg tcattctgca ggcacacatg gacatggttc
      601 cacaaaaaaa cagcaatgtt tcacataatt ttgataccga tccaattcaa gcggttgttg
      661 atggcgattg ggtaagagct caagaaacta cgctgggagc cgacaacggt atcgggctgg
      721 ctgcagcact tactgttttg caatcggaca atattgctca tccggcgctg gaagtgctgg
      781 ttacagtgga cgaagaaacc ggcatgcatg gtgcttttgc tttgaaacca aattttctga
      841 agggagaaat tctcctcaat ctcgattcgg aagatgaagg agaattgtat atcggttgcg
      901 cgggcggatt ggatgccgac attacgttcc gctatcagga agttcctgtt cccgaaagcg
      961 atgtcgcttt aaaaatttct ctgacggggc tgaaaggtgg acattctggt gtggatattc
     1021 acttgcaacg agccaatgcc aacaaattgc tgtttcgttt cctgaaaaaa gccgtagccg
     1081 aatacgaagc tcgcctggcc tgggtggaag gtggaaattt gcgtaatgcc attccacgcg
     1141 aagcttttgc tctcattact attcctgccg acggtgtgga cgatttaatt gaactgttaa
     1201 aagattttga aaatttgttt gtagaagaaa accaatttgt ggaagacaat attcgattgc
     1261 aagtggagaa ggtaaaattg cccaccggct tgatgcccga agaaattcag gatgacctga
     1321 ttaatggtgt aacagcttgc cccaatggtg ttttccgatt catccccgaa gttttcgatg
     1381 tagtggaaac gtccaacaac ctggcagtgg taaaatctga tggctcaacc attcggttac
     1441 gttgtctgct tagaagttcc gtagaatcga gaaaggaaga gttagcttca atggtagaga
     1501 gcgtcttttc actggcaggt gcgcacattg atttttccgg cggttacccc ggctggaaac
     1561 ccaatctgga ctcgccaatt ttaaaggtaa tgaccgaagt ttacgaaaaa caattcggtt
     1621 cgaaaccgaa aatcaaaatc attcatgccg gtttggaatg tggcattatt ggcatgcatt
     1681 atcccgatat ggatatgatt tctttcggac caaccattcg atacccgcat tcgcccgatg
     1741 aaaaagtcaa tattgcttcc gtcgaaaaat tctgggactt tttgcttgca acactgcaaa
     1801 atattccgga aaaataaatt tcagaaaaaa aataaaaaac tcatttcaat aaaacatatg
     1861 aaacccacat gtattgcata cacaaacacg aatgaatata atattcagac atgtgggttc
     1921 catcagacaa ttaaaaaata aattatattc tgatgaaaaa attgacaata atttctatac
     1981 tgattttaag cattttacaa ctttacgccc aaaaagatac gaccgcggtg gacagttttc
     2041 gcttcaccac catcaaagaa aataaaatca cgccaatcaa agaccaggca agctccggaa
     2101 cttgctggag tttttccggc ttggctttta tagaatcgga attgttgaga atggggaaac
     2161 ctgaaacgga tttgtccgaa atgtttattg tgcatcatac ctattccgac aaagccgaca
     2221 aatatgtacg catgaacgga accattaact ttgctgccgg aggttccttt ttcgacgatt
     2281 tgtatgcgtt gaaaaattat ggcattgttc ctgaagccat tatgaacggg ctaaactatg
     2341 gcgaagaaaa gcacaggcac ggtgaattgg atgctctgac ggaagcttat gtaaaggtaa
     2401 tcgttaccaa cccgaacaaa aaattatcta ctgcatggaa gcagggcttt gatggaattc
     2461 tggatgctta tctgggaaaa tgtcccgaaa aattcatctg ggaaggcaga gaatatacac
     2521 cgcttactta ttttcaatca ttgggaatca atcccgacga ttatgtttcg ctcacttcat
     2581 ttacgcatca tcctttttac acccgatttg ccatcgaaat tcccgata
//
LOCUS       DAOTAX010000136         2596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_30785_length_2596_cov_18.953955, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000136 DAOTAX010000000
VERSION     DAOTAX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30785_length_2596_cov_18.953955"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="PLM04_08625"
     CDS             complement(<1..2087)
                     /locus_tag="PLM04_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03170.1"
                     /translation="MKTYTFPQKRGLIFLCLCIGFFLSSAYGTNPDFSNYIKNLSGEV
                     NTENLNDIVPEIAISGNTIHLVWMEYKYNDVNKIYYRRSTDLGKTWEPPKLLLSIRNF
                     DYVYDGCSIKKLSVEGNNVHICIPDYMYSESGTGRLYYIRSTDNGSTFEAPKEIANTG
                     GWYKKIFTSFIKCVNNHIGIAYTSEVDGIHFIYSTDNGDHFKDTLVVKNSEYHSYVVD
                     FDYDGNQAFILHNYTYYSSGFAVGKMYITTSFDNGETFTTTKISKPYTSGDYTYEKSC
                     TTAQTSSTYFRDYRKKMARWDENIYIVFEELKDGNWHTVLVTSNDNGHTFQSPIVFED
                     TNFTQYSDATTRGSTVEARRNHVYIATQIKNSAYLKISDDGGNTFTPAKNYFATIPVE
                     LYAEDKPYLFYLMIDPADSTGETVHFVGNTHFLATSYDGGHTFSKIINYTNCFNGFSM
                     GTQVLIDQFNVKHWFLVHNPYGGTDTDIYYRQTGNEPAPGNENQYFSVVEPKSDNSKI
                     SLGIGHSPTLNFDSAMTAEMWVKFYPQVTHDAPLLLKIKGCCYSDYNPRGYDIAFRNS
                     NGTIALNSGLQTDKGNFVNWCPSEIKDTLWHHVAFTYDANAGLKNFITYVDGVVKAKQ
                     TVTGKIDAGEGMLMLGPNREANHYYELAYKIDEVRLWNTALTQSQLQQNMVRTDFEGE
                     NHLKMYLNFNGTF"
     gene            complement(2198..>2596)
                     /locus_tag="PLM04_08630"
     CDS             complement(2198..>2596)
                     /locus_tag="PLM04_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON03171.1"
                     /translation="TNAEFTAAEKGSTYKFYSICMDSVGYRENAPENFDQEIFYTRVP
                     ETKISYEWKIYPNPVNDKLFIQLVDAPFVDNYIIELVSMNGSRYAVGKYQTQQIREGI
                     SVPVNNLPAGQYLLRIIFDKNNYSEKVTIQ"
BASE COUNT          815 a          473 c          457 g          851 t
ORIGIN      
        1 ttaaatgtgc cgttgaagtt caaatacatt ttcaaatgat tttctccttc aaaatcggtt
       61 cgtaccatgt tttgctgaag ctgactttgc gtaagtgccg tattccaaag gcgaacttca
      121 tcaattttat aagctaattc ataataatga tttgcttctc tgttgggacc caacatcaac
      181 attccttctc cggcatcgat ttttcccgta acagtttgtt tggcttttac tacaccgtcc
      241 acataagtta taaaattttt cagcccggca ttcgcatcgt atgtaaaagc tacatgatgc
      301 cagagcgtat ccttaatttc ggaagggcac cagttgacaa aatttccctt gtcggtttga
      361 agtccgctat taagggctat tgttccattg ctgttccgaa aagcaatatc ataacctctt
      421 gggttataat cagaataaca gcaaccttta attttcaata aaagaggagc atcatgagta
      481 acttgtggat aaaattttac ccacatttcg gccgtcatgg cagaatcgaa atttaatgtg
      541 ggagaatgtc caatgcccag cgaaatttta ctgttatcac ttttaggctc taccacacta
      601 aagtattgat tttcattacc gggagcaggt tcgttgccgg tttgccggta ataaatatcc
      661 gtatcggttc cgccataagg attatgtacc aaaaaccagt gtttgacatt aaattgatca
      721 atcagtactt gcgttcccat cgaaaagccg ttaaaacaat tggtgtaatt gattatttta
      781 ctgaaagtat gaccaccatc atacgaagtt gcaagaaaat gtgtatttcc tacaaaatga
      841 acggtttctc cggtggaatc agcgggatcg atcatcaaat aaaaaaggta gggtttatct
      901 tcagcataca actctacagg aattgttgca aaataatttt ttgcaggtgt gaatgtattt
      961 cctccatcgt ctgaaatttt taaatacgca ctatttttaa tttgtgtggc aatatatacg
     1021 tgatttcttc tggcttcgac tgtagaaccg cgagttgtag catctgaata ttgcgtaaaa
     1081 ttggtatctt caaatacaat aggagactga aaagtatgtc cgttgtcatt ggaagttact
     1141 aaaacagtat gccaatttcc atctttcaat tcttcaaaaa caatataaat attctcgtcc
     1201 catctggcca ttttttttcg gtaatctcta aaatatgtcg aggaagtttg agcggtggtg
     1261 caggattttt catacgtata atcgccactg gtataaggtt ttgaaatctt ggtagttgta
     1321 aaagtttcac cattatcgaa agaggttgtg atgtacattt taccaactgc aaaaccggaa
     1381 gaataatacg tataattatg gaggataaat gcctggttac catcgtaatc aaaatcaact
     1441 acataagaat gatattcaga atttttcact accaatgtat ctttgaagtg atctccatta
     1501 tcggtggaat agataaagtg aattccatca acttcactag tgtaagcaat gccaatatgg
     1561 ttgtttacgc actttataaa acttgtaaaa atttttttgt accaacctcc cgtattggca
     1621 atttcttttg gagcttcaaa tgtgctgcca ttatcggtag aacgaatata atacagtcga
     1681 ccggttccac tctcggaata catataatcc ggaatacaaa tgtgaacatt attaccctct
     1741 accgacagtt tttttataga acatccatca taaacgtaat caaaattccg aatacttaaa
     1801 agcaattttg gtggctccca agttttaccc agatctgtgg atcgacgata ataaattttg
     1861 ttgacatcat tgtacttata ttccatccat accagatgaa tggtattgcc actgatagca
     1921 atttcgggta caatatcgtt caagttttcc gtattcactt ctccagacag atttttaata
     1981 taattggaaa aatctggatt tgtcccataa gcagaagaga gaaaaaagcc tatgcacaag
     2041 cacaaaaaga taagacccct tttttgtgga aaagtgtaag ttttcatgat tgcttgtttt
     2101 aaataagtta agaaaactat tcagtaaaaa atagaacagc agatgaaagg gttagatgaa
     2161 acagaatttt aattcatcta tttagcacag gaatccatta ttgaattgta actttttcag
     2221 agtagttgtt cttgtcaaaa ataattctca acaaatattg tcccgcaggt aaattattga
     2281 ctggcacaga aataccttct ctgatttgtt gggtttgata tttacctaca gcatatcgac
     2341 tgccattcat actaaccagt tcaatgatat aattatctac aaaaggagca tctaccagtt
     2401 gaatgaaaag tttgtcgtta actggattag gataaatttt ccactcatac gaaatttttg
     2461 tttcaggaac gcgtgtgtag aaaatttcct ggtcaaaatt ttcaggtgca ttttcccgat
     2521 aacctacgct atccatgcaa atgctgtaaa atttgtaggt tgaacctttt tcggcagcag
     2581 taaattctgc attggt
//
LOCUS       DAOTAX010000137         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_30933_length_2584_cov_34.465006, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000137 DAOTAX010000000
VERSION     DAOTAX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30933_length_2584_cov_34.465006"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..>2584)
                     /locus_tag="PLM04_08635"
     CDS             complement(150..>2584)
                     /locus_tag="PLM04_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HON03172.1"
                     /translation="NDDSNFKVLLTSSDQMYKDITPEIQAKLPVYSGDLLLIEHSSGS
                     LTSQAFMKRMNRKNELLASSAELASVAADWMGTAAYPFGKLNNGWDLVLGSQFHDILP
                     GTSIPKAYEYAWNDEFIAANCFAEALKNSLSSIAGKMNTQTEGKAIVVYNPVSRARQD
                     VVTALLEYKSLPQTVGVFDKNGKEVPSQIIERNGNGIKILFLADMPSASIKVYDVREI
                     SNAVKQTALSISENSLENEYYKVKVAPNGDILSIYDKKNTKELLAKPARLEFLAETPD
                     EWPAWNMDWKDRQKPAIDFMDKNAKISVVEQGPVRVTLKVERAGQNSTITQFYSLAAG
                     EAGKRLEVSNKIDWQSRAVSLKAAFPLSVSNKNSTYNLGVGTISRGNNEEKKFEVPSK
                     QWFDLTDQSGKYGVTILEDCKYGSDKPDDNTLRLTLLYTPGIHLPQWQRTMYQSTQDW
                     GIHDVKYAVYGHQGDWRKGESAWQAQFFNQPLMAFESTKHDGTLGNSFSMISISRPEV
                     GLMAFKKMESGEGYVIRVNELSGKDIKNAKITLPDEILQAYEVNGQEKRIGDVNFTKN
                     SIQFDLSHYTIKSFAVKLKKANEAVLPQQPVTIPYNGDVMSYDENRDDGVMFDGQSFP
                     AELLPEYVESEGIRFKIGNKQDEQNNAVACNAQTIALPAGNFTKLYLLAAAANDVKAD
                     FYVDGNPINLSIQKWTGFVGQFYNRILSLDQNKVLKMNEPFTKTDNIAWFASHCHNNY
                     PMKNEAYQYSYLYKYEIPIVAGAKNLTLPNNRNIKIVAITVAAPKADNLKPLQAMYDD
                     FENNPTFHLREK"
BASE COUNT          715 a          543 c          463 g          863 t
ORIGIN      
        1 agacatgtgg gttccatcag acctttaaaa aataaattat attctgatga aatttctctg
       61 taaatctttt gacaagtttt aaagagaaat ttatcaattc ggtttttgtg ctatttatcg
      121 aaattatcaa tagcaatcac ctttaaaaat tatttttctc tcaaatgaaa tgtcggatta
      181 ttctcgaaat cgtcatacat tgcctgaaga ggttttaaat tgtcagcttt tggagcagcc
      241 accgtaatgg caacaatttt tatatttctg ttgtttggta aagtcaaatt cttggctcct
      301 gcaacaattg gaatttcgta tttatacaga taactgtact gataagcttc gtttttcatc
      361 ggataattgt tgtggcaatg cgaagcgaac catgcgatat tatccgtctt tgtaaaaggc
      421 tcattcattt tcagcacttt attctgatcc aaactcagaa tacgattgta gaattgaccg
      481 acaaaacctg tccatttttg aatcgacaga ttgattgggt taccatccac ataaaaatcg
      541 gctttcacat catttgcagc agcagccaaa agataaagtt tggtaaaatt tccggcaggc
      601 aaagcaatgg tttgagcgtt gcaagcaaca gcattatttt gttcgtcctg tttattacca
      661 attttgaaac gaatgccttc gctttcgaca tattccggca aaagttcggc aggaaacgac
      721 tgaccatcga acatgactcc gtcgtcgcga ttttcgtcgt aactcattac atccccattg
      781 taaggaatag ttaccggttg ctgtggcaat acagcttcat ttgctttctt aagtttgacg
      841 gcaaaacttt ttatcgtata atgacttaaa tcgaattgaa tgctgttttt tgtaaaattt
      901 acatcgccta ttcgtttttc ctgtccgttc acttcataag cttgcagtat ttcgtccggc
      961 aatgtgattt tcgcattttt tatatctttt cccgacaatt cattcacacg aataacgtat
     1021 ccttcgcccg attccatttt tttgaaagcc attaaaccta cttcgggacg actgatcgaa
     1081 atcatcgaga aactgttgcc caatgttcca tcgtgtttgg tggattcaaa agccattaaa
     1141 ggctgattga aaaattgagc ttgccatgct gattctcctt ttctccaatc accttggtgt
     1201 ccataaacgg cgtatttcac atcatgaata ccccaatcct gagtactttg atacattgtc
     1261 ctctgccact ggggtaaatg aataccgggc gtgtaaagta aagtcagacg caaggtattg
     1321 tcatccggtt tgtcggaacc atatttgcaa tcttccaaaa tcgttacgcc atattttccc
     1381 gattgatcag tcaagtcgaa ccattgtttg gagggaactt caaatttttt ctcctcattg
     1441 tttcctcgag aaatggtacc aacacctaaa ttgtaggttg aatttttatt ggaaacggat
     1501 agaggaaaag ctgctttcaa actgacagca cgtgattgcc agtcaatctt gttgctcact
     1561 tccaaacgtt tacctgcttc accggcagcc aaactgtaga attgcgtgat ggtcgaattt
     1621 tgtcctgctc tttccacttt caaagttacc cttaccggcc cttgttcgac aacggatatt
     1681 tttgcatttt tatccataaa gtcaatagcc ggtttttgtc gatctttcca gtccatgttc
     1741 catgccggcc attcgtcggg tgtttctgcc aaaaattcaa gacgagccgg ttttgccaac
     1801 aattctttcg tatttttttt atcataaata ctgagaatat cgccattggg agctactttc
     1861 actttgtagt actcgttttc aagtgaattt tcggaaatag aaagtgcagt ttgttttact
     1921 gcatttgaaa tttcacgtac atcatacact tttatgcttg cagaaggcat gtcggccaaa
     1981 aacaaaattt taataccatt tccatttctt tcaataattt gcgaaggaac ttcctttcca
     2041 tttttatcga aaacaccgac ggtttgtggt aacgatttgt attccaatag agcggtaaca
     2101 acatcttgtc ttgcccgact taccggattg taaactacaa tggcttttcc ttcagtttgt
     2161 gtattcattt taccggcaat ggaactcagg ctgtttttga gcgcttcagc aaaacaattg
     2221 gcagcgataa attcatcatt ccatgcatat tcataggctt ttggaataga cgttcccggc
     2281 aaaatgtcat gaaattggct tcccagcacc aaatcccaac cattgtttag tttaccgaac
     2341 ggataagctg ctgttcccat ccaatcggca gcgacacttg caagttctgc cgacgaagcc
     2401 aaaagttcgt ttttacggtt cattcttttc ataaaagctt gagaagtcaa tgaacccgaa
     2461 ctgtgctcta tcaaaagtaa atcgcccgaa tataccggaa gttttgcctg aatttcaggc
     2521 gtaatatctt tgtacatttg atcggaagaa gtcaacaaaa ccttaaaatt actgtcgtca
     2581 ttgt
//
LOCUS       DAOTAX010000138         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_31081_length_2574_cov_29.522827, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000138 DAOTAX010000000
VERSION     DAOTAX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31081_length_2574_cov_29.522827"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..646
                     /locus_tag="PLM04_08640"
     CDS             200..646
                     /locus_tag="PLM04_08640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HON03173.1"
                     /translation="MKTESLKKNIFVRRAHPTDVDAIHHILDMEPFKYDENLPYDRSW
                     LVQLVENERCLTLVYESENTVKGFISGEKMVSNAVMIWFCAVKKEFQNSIIGIRLYME
                     FEKICRENGVTGILAYGYKTSAGMLKRLNFSTDGKMYQEFFKILKE"
     gene            complement(778..>2574)
                     /gene="secF"
                     /locus_tag="PLM04_08645"
     CDS             complement(778..>2574)
                     /gene="secF"
                     /locus_tag="PLM04_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HON03174.1"
                     /translation="AKTWARMTRDNIGKSIAIVLDGYVYSYPTVQNEITGGSSQITGN
                     FTVQEAKDLANVLKSGKMPAPAHIVQEDVIGPTLGKEAIQAGLISFIIAFILILLYMQ
                     IYYGLIPGLVVDTALLLNVFLILGIMASFQAVLTLPGMAGIVLTLGMAVDANVLIYER
                     IKEELRAGKNMKNALVSGYRNSLSAIIDGNVTTLLTGIILYIFGTGPIRGFATTLIIG
                     LITSVFTSVFVTRIIFESFHAKDKWLDLKFYTKLTKSLLLHPKIDFIGKRKYGYIFSG
                     AILTLFVIALFTKGLTQGVDFSGGRNYVVRFDQPVRTDEIKNSLEDYFQNASLSVITI
                     GNDKQVRINTNYKIDDDNAAVDDEISDLLYNGLKTYLPAGTTKDQFVHENIVNSQKVG
                     PSIADDVKTSAYIAVFIAIIVIGLYILMRFRNVAFSVGTIVALFHDVLFIIGCYCVFS
                     GLVPFSLEVDQNFVAALLTIIGYSVNDTVVIFDRIRENLGYYPKRNKKRLMNEALNQT
                     LVRTFSTSLTVFITIGTIFLFGGETIRGFSFAMLIGTVIGVYSTLFIACPIAYEIQKK
                     QLKITDDDYDDEKPLLKKPEIKSQPDRKKKDK"
BASE COUNT          822 a          496 c          492 g          764 t
ORIGIN      
        1 tgaacaacag accaagaaaa aaaattaaat ttcatcagaa tataatttat tttttaaagg
       61 tctgatggaa cccacatgtc tgaatattct attcattcgt gtttgtgtat gcaaaacctg
      121 tgggtttctt aatgcccaat gaatttttat tgctaaattt gtctaaccaa aaagttgcat
      181 ttgtaatttg tattcagcca tgaaaaccga aagtctaaag aaaaacatat ttgtccgtcg
      241 tgcacaccca actgatgtgg atgctattca ccatattttg gatatggagc cattcaaata
      301 cgacgaaaat ttaccctacg accgttcgtg gctggtgcag ttggttgaaa acgaacgttg
      361 tttgacgctc gtttacgaaa gcgaaaatac ggtaaaagga tttatcagcg gggaaaagat
      421 ggtgagtaat gcggtaatga tttggttttg tgccgtaaaa aaggaatttc aaaacagcat
      481 cataggcatt aggctttata tggaatttga aaaaatttgt cgggaaaatg gtgtaaccgg
      541 tattttggca tacggataca aaacttccgc aggtatgttg aagcgactga atttttccac
      601 agatggaaaa atgtatcagg agtttttcaa aatattgaaa gaataaagaa caaatgcatt
      661 taacccatcc ctaatttttt tttgtacatc cctcaaaaaa ttttccttct ctttgtgatc
      721 gggtaaaatg aataaccccg agtgaagttc tcggggttat tcattgaaca atgattgtta
      781 tttgtctttt ttctttctgt cgggctggct tttgatttca ggttttttaa gcaacggttt
      841 ttcgtcatca taatcgtcat ctgtaatctt caactgcttc ttctgaattt cataagcaat
      901 aggacaagca ataaacaatg tagaataaac cccaatgaca gtacctatca gcatagcaaa
      961 cgaaaaaccg cgaatggttt caccgccaaa caggaagatg gtgccgatgg taataaacac
     1021 cgtcaaggaa gtgctgaagg tacgaaccaa tgtctgattg agagcttcgt tcatcaatcg
     1081 tttcttgttt cgtttgggat aatatccgag attttcacga atacggtcga agataaccac
     1141 cgtatcgtta accgaatatc cgataatggt cagcaaggca gcaacaaagt tctggtcgac
     1201 ttccagggag aatggtacca acccactgaa tacgcaataa catccaatga taaagagtac
     1261 atcgtggaaa agggcgacaa tggttccaac actgaaagcc acatttctaa atcgcatcaa
     1321 aatgtataga ccgataacta tgatagctat gaatactgca atgtatgcag aagtttttac
     1381 gtcatccgca atggatggcc ccactttttg agaattgaca atattctcgt gtacaaattg
     1441 gtctttagta gttcctgccg gcagatatgt tttcaaaccg ttgtacagta aatcgctgat
     1501 ttcgtcatcg acagctgcat tgtcatcatc aattttgtaa ttcgtgttga tacgcacttg
     1561 tttgtcgttg ccaatcgtaa ttacagataa agaagcattt tggaaataat cttccaaact
     1621 gttttttatt tcgtcggtac gaaccggttg atcaaaacgc acaacatagt ttcttcctcc
     1681 ggagaaatcg actccttgtg tcaatccctt agtaaacaat gcgataacga acaaagttaa
     1741 aattgctcct gaaaaaatgt aaccatattt gcgtttgcca ataaaatcga ttttgggatg
     1801 caggagcaag ctctttgtca atttggtata aaacttcaaa tcgagccatt tgtcttttgc
     1861 atggaacgat tcaaagataa ttcttgttac aaaaacgctg gtaaataccg aagtaatcaa
     1921 accaataatc aaagtggtag caaatccacg aataggtcca gttccgaaaa tatacaaaat
     1981 aattccggtc aacagcgtgg taacatttcc atcaataatg gccgataaag aatttctgta
     2041 accactgacg agcgcatttt tcatgttttt acccgctcga agttcttcct ttatacgctc
     2101 atatatcaat acattggcat ccacagccat tcctaaagtg agcacaatac cggccattcc
     2161 aggcaacgtg agtacagctt gaaatgaagc cataatgcca agaatcaaga aaacgttcaa
     2221 aagcagagca gtgtcgacta caagtccagg aattaatccg taatagattt gcatgtaaag
     2281 caaaatcagg ataaacgcaa taatgaaaga aataagtccg gcttgaatcg cttcttttcc
     2341 caaagttgga ccaatgacat cttcttggac aatgtgagcg ggtgcaggca tttttcctga
     2401 tttcaatacg ttggccaagt ctttggcttc ctgaactgta aagtttccgg ttatttgaga
     2461 actgcctcct gtaatctcgt tttgaacagt aggataagaa tatacatatc cgtccagaac
     2521 tatcgcaata gacttaccaa tattgtcccg cgtcattctt gcccatgttt tggc
//
LOCUS       DAOTAX010000139         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_31364_length_2556_cov_36.615354, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000139 DAOTAX010000000
VERSION     DAOTAX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31364_length_2556_cov_36.615354"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1033)
                     /locus_tag="PLM04_08650"
     CDS             complement(380..1033)
                     /locus_tag="PLM04_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03175.1"
                     /translation="MKKEEIKLSLILVVGGLGMIFCLFFLDIPFLPNLKYKSEVLDIQ
                     KYGRPFDRSSYQPHPLPKSSYKSNRTYSSDELMTEQISADIPGRGFQSEANGYSLGNG
                     STTVGSTFSQKLRSNTSSQGMGSGGGYYAYGKRGSSESRVSGQVSKGSMFAASELREP
                     FSNSTPAPQRAPYAPNQGGTDPGGDPLGNPIPVGEGWGVLLLLAAGYAFIMKLRAKR"
     gene            complement(1046..>2556)
                     /locus_tag="PLM04_08655"
     CDS             complement(1046..>2556)
                     /locus_tag="PLM04_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HON03176.1"
                     /translation="ATNKKLIIPANKSLTVNNTITISDAQNPNLIQIQSGGSSTPQGT
                     LIFHNDAAHPVYATVEMYSKAWIDPNGVTNNKYFWQYFGLPFRSLQANPSFYGAYVRR
                     WDETGTTISNHWVQLGNNDVIQSFLGYEICQPSAKTYTLQGILENRDFSSGELAYTTT
                     ALYPGQHIFGNSYTAAIDITQINFGSNMEATVYLYNTGSYNNWVTNNGETSHSPSNTN
                     PGTYAAIPKNQAGQGGIQGQIPSMQGFLVKAMSGSSGNTISIPYSSVAVKNTTLQRAK
                     AAEENDSEKKQYLRIDLKTDTVIVDRMWIFNEENCTRSFDNGWDGPKMLPASGTPVLC
                     AIEKDGLYQVNSVNDMDETKLGFRKGTETEYSLTFYPKNLQQRYPAVYLLDEAVNQAV
                     KIENETVYSFTATGTENLQQRFRIVTRYYEKGEPEEASQIKVFSSKSHIVIDNLSAEN
                     GEALIYDMAGRKVGQEKFFSETITMFGDYPTGVYVVKAVSNKETVTKRIIVQ"
BASE COUNT          722 a          530 c          511 g          793 t
ORIGIN      
        1 gtaatacatg tgggtttcat acgaaaaaga tttatcaaaa aaatttaaaa acagagtaag
       61 ataaggcaat tgatataatg aaaatttttt gaaagcaaaa tttcgatttc aaaacaggaa
      121 aaagttcagt caaaatgtaa ttatataaaa ttctgtgttc agttagaatt tttttaaaca
      181 acacaagtag aaaaaattaa aaagtaaaaa ttaatgtcct taaaaatgtt taggagagca
      241 gcaactttat ttttttacaa acacattatt atacaggcga gttggaaaaa tttttaaaaa
      301 acgatttgaa atcgaaatag aaagaaaaaa tttcgggata ggtatgttgg agcttgatat
      361 tcgaacattc aaacaaaaac tatctttttg ctcttaactt cataataaaa gcataaccgg
      421 cagccagcag caacagtact ccccatcctt caccaaccgg tatgggatta cccaatggat
      481 cgccgcccgg atctgtacca ccctggtttg gtgcataagg agctctttgt ggagccggtg
      541 tactgtttga aaacggttct ctcaactcgc tcgccgcaaa catacttcct tttgatactt
      601 ggccggaaac acgactttcg gaagaacctc ttttcccata agcgtaatat ccaccaccgc
      661 tgcccattcc ttgtgaggaa gtatttgatc tgagtttttg ggaaaatgtg gaacccactg
      721 ttgtggatcc attgcctaag ctgtatccat ttgcttcgct ctgaaaacct ctgcccggaa
      781 tatcggcact tatttgttcg gtcattaatt catcagaaga ataagttcta ttacttttat
      841 agcttgattt tggaagtgga tgaggttgat aggaagaacg atcgaaaggt ctgccatatt
      901 tttgtatatc cagcacctca gacttgtatt tcaagttggg caaaaaggga atatccagaa
      961 agaaaaggca aaaaatcatt cccagccctc caaccactaa tatcaacgac agttttattt
     1021 cttccttttt catttctctc cgcttttact gaacaatgat tcgttttgtt accgtttcct
     1081 tatttgaaac ggcttttacc acataaactc ccgttgggta atcgccaaac atggtaatgg
     1141 tttcgctaaa gaatttctcc tgacccactt ttcgtcccgc catatcgtaa atcaatgctt
     1201 ctccattttc tgcactcaga ttgtcgatca caatatggct tttggaagaa aataccttga
     1261 tttgcgatgc ctcttccggt tctccttttt cgtaataacg cgttacaata cgaaaacgct
     1321 gttgcaaatt ttccgttcct gtagccgtga atgaataaac cgtttcattt tctattttta
     1381 ccgcttggtt tactgcctcg tccagcagat aaacggccgg atagcgctgc tgtagatttt
     1441 tcggataaaa tgttaaggag tattctgttt cggttccttt ccggaatccg agtttggttt
     1501 cgtccatatc gtttaccgag ttcacttgat acaaaccgtc cttttctatg gcacacaata
     1561 ccggtgttcc acttgccgga agcatttttg gcccgtccca gccattgtcg aaagaacggg
     1621 tacagttttc ttcgttgaaa atccacatgc ggtctacaat tacggtatcc gtttttaagt
     1681 ctatgcgcag atattgtttt ttctccgaat cgttttcttc agcagccttt gcacgttgaa
     1741 gggtggtgtt tttcacggct acggaagaat agggaatgga aatggtattt cctgacgaac
     1801 cgctcatggc ttttaccaga aatccctgca tggagggaat ttgtccctga atgcctccct
     1861 gaccggcttg attcttggga atggcggcat atgttcccgg gttggtgttg ctcggagaat
     1921 gactcgtttc accattattg gttacccagt tgttgtaaga acccgtgttg tacagataga
     1981 cagttgcttc catattgctg ccaaaattaa tttgggtgat atcaatagct gccgtatagg
     2041 aattgccaaa tatgtgctga ccgggataca aggcggtagt ggtgtaagcc aattcgccgg
     2101 aactgaaatc tcgattttcc aaaattccct gcaaggtgta ggtttttgct gaaggctgac
     2161 aaatttcata gcccaaaaac gactgaatca catcgttgtt gcccaattga acccaatgat
     2221 tggaaatcgt tgttcccgtt tcatcccagc gtcttacata agcgccatag aaagaaggat
     2281 tggcctgcag cgaacggaaa ggcaaaccga aatattgcca gaaatacttg ttattggtta
     2341 ccccattcgg atcaatccat gctttggaat acatttctac cgtagcatat acaggatgag
     2401 cagcatcatt atggaaaatc aacgttcctt gcggagtgga tgaaccaccc gactggatct
     2461 gaatcaaatt cggattctgt gcatcactga tagtaattgt gttgtttacc gttaagcttt
     2521 tgttggccgg aataatcaac ttcttgttgg tagcat
//
LOCUS       DAOTAX010000140         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_31886_length_2522_cov_25.832590, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000140 DAOTAX010000000
VERSION     DAOTAX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31886_length_2522_cov_25.832590"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLM04_08660"
     CDS             <1..761
                     /locus_tag="PLM04_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03177.1"
                     /translation="GMLENGKTYVLVHKSCSNNDLKAKANLLTDSLLSFNGNDAVALL
                     RNGVFIDVVGIANAGADVYWGQDLTLKRKATVTHPSSQFRDIEWNFYGIDDFQMLGNH
                     QMNLQPSSNYLLNDFETGKTDHFTISQLLPENTYTYSVTAIYPDEEKKALNTMQIHTS
                     KLEVPVIMNATDVRANSFVAGWEDCLYTSDYLLDVFQIQSGKDTTETESFDNIGSNGK
                     PLPEGWSGTASGNYTTAASSGKNPNALAFKNDGE"
     gene            807..1427
                     /locus_tag="PLM04_08665"
     CDS             807..1427
                     /locus_tag="PLM04_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HON03178.1"
                     /translation="MYRFPSGATGSSLIVEAVKNGNYVRIDSIGSVNTSKNYPMYTFQ
                     ESDDIHSFRFIYKKVSGNLALDDVEATYKQQDTVFVLKNFQVSGSQFKVEGLNPNSVY
                     FYRVRSTIHPYTSDFSETIQVKTGFPSALENIPASQYKIYISSGDIKLSNLQGNERVQ
                     LFNIAGVCLFDQHSSGSEMSIRIPHRGIFIVRISNKDFTFARKLIK"
     gene            1442..>2522
                     /locus_tag="PLM04_08670"
     CDS             1442..>2522
                     /locus_tag="PLM04_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HON03179.1"
                     /translation="MKFSFCFIKKISVVVLTFFSLTLSAEIPEGYYRTAEGKTGAELK
                     TALHNIIRQHVILNYDTSTTVWWYTYFKTTDWHPDGYFWDMYSNNKRSTYNSSVMNRE
                     HCMPRSWWGTSSNYSTFDANGDLVNLSPSDAEANSAKSNYPLGIVGTASFDNGVVKVG
                     NNTYPGYTGLVFEPANQYKGDFARTYMYMVTCYEDYHSYWRGTGTSSMLDRNTYPVFK
                     QWAIKMLLEWSRNDPVSDKEINRNDGVYRIQQNRNPFIDHPELAEYIWGTSKGEVWNG
                     TAGNNDLYVNLSVYPNPASTSVLVNLNKPESSYYRIYDLSGISVKIGNFSSDGKLDVS
                     ELQIGSYLLVVYYGNQRKTIKLMIAH"
BASE COUNT          784 a          467 c          530 g          741 t
ORIGIN      
        1 atggaatgct ggaaaacgga aaaacttatg tgttggttca caaaagctgt tcaaacaacg
       61 atttgaaagc aaaagccaat ttgttaacgg attcattact cagttttaat ggaaacgatg
      121 ccgttgcttt gttgagaaat ggtgttttta ttgatgttgt aggcatcgcc aatgcaggtg
      181 ctgatgtata ttggggtcag gatttaactt tgaaacgaaa agctacggtt acgcatccaa
      241 gttctcaatt cagagatata gaatggaatt tttacggaat agatgatttt caaatgcttg
      301 gaaatcatca aatgaactta caaccgtcct ccaactatct gttaaatgat tttgagaccg
      361 ggaaaacgga tcatttcacc atatcgcaat tgttacccga aaatacttat acttattccg
      421 taacggcaat atatcccgat gaagaaaaaa aagctttgaa caccatgcag attcatactt
      481 ccaaacttga agtgcctgta attatgaatg caacggatgt tcgagccaac agttttgttg
      541 ccggttggga agattgcctg tacacgtccg attatctgct ggatgttttt caaatacagt
      601 ccggcaaaga tacaactgaa accgaatcgt ttgacaatat tggaagcaac gggaaacctt
      661 tgcctgaagg gtggagtgga acagcaagcg gaaattatac taccgctgcc agctccggta
      721 aaaatcccaa cgcgttggca tttaaaaatg atggagaata gttacaaacc aaaacttatc
      781 cgagctgggt ttcgcaacta aaatttatgt atcgtttccc ttcaggagca accggttcat
      841 cgcttattgt cgaagcagta aagaatggaa attatgttcg tatcgacagc attggatcgg
      901 taaatacttc aaaaaattat ccgatgtaca cttttcagga atcggatgat attcattcct
      961 ttaggtttat ttacaaaaaa gtatcaggca acctggcact tgacgatgtg gaggcaactt
     1021 acaagcaaca ggacacagtt tttgtgttga aaaattttca ggtttcggga agtcaattta
     1081 aagtggaagg gctgaatccg aattcagttt atttttatcg ggttcgttca accatacatc
     1141 catatacatc ggatttttct gaaactattc aggtgaagac cggatttcct tcagctttgg
     1201 aaaatatacc tgcaagccaa tataaaattt acatatccag tggcgatatt aaattgtcga
     1261 acttgcaagg caatgagcgt gttcaacttt ttaatattgc cggcgtatgt ttgtttgatc
     1321 aacattcatc aggatcggag atgagtataa gaattccgca cagagggatt tttattgttc
     1381 gtatctcaaa taaagatttt acttttgcac gaaaattaat aaaataaatt tttcttagta
     1441 catgaaattt tctttttgct ttattaaaaa aatttcagta gttgttctga cgtttttcag
     1501 ccttactctt tcggccgaaa tacctgaagg ttattaccgg actgctgaag gaaaaacagg
     1561 ggctgaatta aaaactgcct tgcacaatat cattcgacaa cacgttattc tgaattacga
     1621 caccagtact accgtttggt ggtacactta ttttaagaca accgactggc atccggacgg
     1681 ttatttctgg gacatgtatt ccaacaacaa acgttccact tataatagca gcgttatgaa
     1741 tcgtgaacac tgcatgccgc gaagctggtg gggaacaagt tcgaattatt caacattcga
     1801 tgccaatggc gatttggtga atctttctcc ttcggatgcc gaagccaact cggccaaatc
     1861 taattatcca ttgggaattg ttggtactgc cagtttcgat aatggggtgg taaaagttgg
     1921 aaacaatact tatcccggtt acaccgggtt ggtttttgag ccggctaatc aatacaaagg
     1981 cgattttgca cgaacttaca tgtatatggt tacctgttac gaagattatc attcgtactg
     2041 gagaggaacc ggaacttcga gcatgttgga cagaaatact tatcccgtat tcaaacaatg
     2101 ggccataaaa atgttgctgg agtggagcag aaatgacccg gtcagcgaca aggaaatcaa
     2161 ccggaatgat ggtgtttatc gaattcaaca gaatcggaat ccatttattg accatcctga
     2221 actggcagaa tacatttggg gcacatcaaa aggcgaggta tggaacggaa cagccggcaa
     2281 caatgacctg tatgtgaacc tgtcggttta tcccaatccg gcatcgactt ctgtgttggt
     2341 taacctcaac aaacccgaaa gttcatatta tcgtatttac gacctcagcg gaataagtgt
     2401 gaaaatcggt aatttctctt cagatgggaa attggatgtt tccgaacttc aaattggttc
     2461 gtaccttttg gtggtatatt atggaaatca gcgaaaaaca ataaagttaa tgatagctca
     2521 ct
//
LOCUS       DAOTAX010000141         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_32774_length_2466_cov_29.804645, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000141 DAOTAX010000000
VERSION     DAOTAX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32774_length_2466_cov_29.804645"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..2082
                     /locus_tag="PLM04_08675"
     CDS             439..2082
                     /locus_tag="PLM04_08675"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HON03180.1"
                     /translation="MRKLLLLIVSVLAYFNSMACTNFLVGKAATVDGSTLISYSADSY
                     FMFGSLYYSPAATYPDNAMLDIYEWDTGEYLGKIKQAERTYSVVGNMNEFQVSIGETT
                     FGGRPELVDTTAILDYGSLIYIALQRSRSAREAIKVMTDLVEQYGYRSEGESFSIADP
                     NEVWIMEMVGKGNRNKGAVWVAQRIPDDCVSAHANQARITTFPFDDKNNCLYAEDVIA
                     FAREKGYFKGKNKDFSFSDTYAPLDYLGLRICEARVWTFFRKVDPSMDSYISYIKGET
                     AQRMPLWIKPNRKISAQDVKEFMRDQYEGTELDITKGIGAGPFHSKLRCSPLTFYVDS
                     VEYVHERPVATQQTGFSFVAQMRSWLPDYIGGILWFGVDDAASSIYVPMYCGINSVPE
                     CYRTGNGSLVEYSPTSAFWIYNQVANFAYSKYSFMMQDIKKVQSKWEQDFNSLVPTID
                     KVALGMSEESAKSFLTNFSNYQAEASTAAWKKLGEYLLVKYMDGNVKMEKDGKFLQND
                     KGIPPGIIRPGYPEDFLRMMVKENPGLRVKTAEELKNRK"
BASE COUNT          758 a          427 c          504 g          777 t
ORIGIN      
        1 agaaaaagaa aaacacacaa acgaggctaa cttttgtctc aaactattta gttaaaactg
       61 tccaaagaat agggggtcaa accacttttg ttgaaaaacg atcaaaaaaa ggaaaaaact
      121 tttccttgaa tttttctatc attctcttta cagattacct attttattaa ttatttaccg
      181 aggtatcact atcttaaaaa tgttattata gaattaatac tttgactatt aacaaattgt
      241 ttaacaaaat attttacttc catatactgc attttttatt gctaaccatt gatttgatgc
      301 gaaaaatatt agaattgatt tctcagtaga ttttagcctt ctttttgatc tataagtttc
      361 ttgtgtcctc tctccctgat gattttgaat ttacatttat ataaactaaa tttgtaattc
      421 ttaaaattta acacaaaaat gagaaaactc cttcttctaa ttgtttccgt attagcttat
      481 ttcaattcaa tggcttgtac caattttctg gttgggaaag ctgcaacggt tgatggttca
      541 acccttatca gttactctgc cgattcttat ttcatgtttg gttctctgta ctattcaccg
      601 gcagcaactt atcccgacaa tgccatgctc gatatttatg agtgggacac gggtgagtat
      661 ttgggaaaaa tcaaacaggc cgaaagaacc tattccgttg ttggcaatat gaatgaattt
      721 caggtttcaa ttggcgaaac tacttttggc ggacgtcccg aactggtcga tacaactgca
      781 attctcgatt atggaagttt gatttatatt gcgttgcaac gttcccgatc tgcccgtgaa
      841 gccataaagg taatgaccga tttggttgag caatatggtt atcgaagtga aggagaatct
      901 ttttccattg ccgacccgaa tgaagtttgg attatggaaa tggtgggaaa aggaaatagg
      961 aataagggtg cagtatgggt tgcacagcgt attccggacg attgtgtttc ggctcatgcc
     1021 aaccaggcca gaatcactac ttttcctttc gatgataaaa acaattgtct ttatgcggaa
     1081 gatgtcatag cttttgctcg tgaaaaaggt tattttaagg gaaaaaacaa ggatttcagt
     1141 ttctcggata cctatgctcc tttggattat ttgggactga gaatttgtga agcgcgggtt
     1201 tggacttttt tccgaaaagt tgatccgtca atggacagtt atatttctta catcaaggga
     1261 gagactgctc agcggatgcc tctgtggata aaacccaacc gtaaaatcag tgctcaggat
     1321 gtaaaggaat ttatgcgtga tcagtacgaa ggaaccgaat tggacattac caaaggaatt
     1381 ggtgccggac ctttccacag caagttgcga tgcagtcctc ttacttttta tgtggacagt
     1441 gtggaatatg tgcacgaacg tcctgtagct actcaacaaa caggtttttc gtttgtggct
     1501 caaatgcgta gttggctgcc cgattatatt ggcggaatat tgtggtttgg cgttgacgat
     1561 gctgccagtt ccatatatgt gcccatgtat tgcggtatca acagtgtacc ggaatgttat
     1621 cgtacaggaa atggaagtct ggtagaatat tccccaactt cggcattctg gatttacaat
     1681 caggttgcca attttgctta cagcaagtat tcttttatga tgcaggatat taaaaaagtt
     1741 cagtcgaaat gggaacagga ttttaattca cttgttccca ctattgacaa ggtggctttg
     1801 gggatgtctg aagaatcggc taaatcgttt ttgaccaatt tcagcaatta tcaggcagaa
     1861 gcttcaacgg cagcatggaa aaagctgggt gaatatttgc ttgtaaaata catggatggt
     1921 aacgttaaaa tggaaaagga cggaaaattt cttcaaaatg acaaaggtat tcctcccgga
     1981 attattcgcc ccggttatcc tgaagacttt ttgcgtatga tggtgaagga gaatccaggg
     2041 ttgagggtga aaacggcaga agaattaaaa aaccgaaaat aaacctcctc ttactttttc
     2101 ttatcctgaa aatccctgct gtccgaaaaa aacaaaataa gttataataa aaaggaaagt
     2161 tacccaaatt tgatgacttc accggcacat aatttttttg ttttaccaca aacataaaaa
     2221 atatagaggc aaaaagcgga aataaatttg gtcttacggc gggactttca gattaaattt
     2281 tatgaagttc ttttgattga ttctaacaca ataaggctgt ttagcaatat tctcaaagga
     2341 gttggacgac cccccccaaa gagccgatat gaaaaagacc ttttccattt ggcaacatta
     2401 gtacggatag atttgatcag cttgtgggat gtttatgaat tgatctggga acaaaaagac
     2461 ctttaa
//
LOCUS       DAOTAX010000142         2442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_33180_length_2442_cov_26.055300, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000142 DAOTAX010000000
VERSION     DAOTAX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33180_length_2442_cov_26.055300"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLM04_08680"
     CDS             complement(<1..641)
                     /locus_tag="PLM04_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03181.1"
                     /translation="MPKTKSFIFISILSFVAIFALLSFLSRSTLAIESYGDEEVCLVN
                     GCDWDDVNNRCICPNVSPTRTPTATPSGTRTPAPTFTLPPETITPFPTPTPTPSSNLS
                     DWCKKSYCGGCNQSECISTALLPQINMPINEVCEWRDNFCYTQMMSSDKIPRISNRKI
                     NPPPARAEINSDGSQYFWNNNYSVQRDRAWILAPSQVVTFTETGPKEYLCTNP"
     gene            complement(764..1903)
                     /locus_tag="PLM04_08685"
     CDS             complement(764..1903)
                     /locus_tag="PLM04_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03182.1"
                     /translation="MKKKKKRFLLFLIVMLLGFSLAWAGDNPKEKKVYFRFSVGYGLP
                     LGTDSVRPSWDLGYTTVYVGQTGYSWPLDPLNEVWFCERWFQCRPQMAPAPTDYYYFV
                     LNGEVPDFYFKPGDRTVYNGYSRSDYKNVIPQINLGIDLRITKGVFFAVDVFARQDQL
                     KVQEEGYILYVKQVWGPYYELPAPDGHYQDTDWYLDTVMEHPKVEMVMDLWNFGINPS
                     LKFEIPISRNFSIFPRFGVALFMSKAKVQSDWTLNFLYPYDYVFMYHQKPNYFGDISW
                     QIKQNKTEFSLAPVVGLDFGNNIFFNVELQLGSGKNFSVTNDDSEHLYKGLLLYNNNS
                     QKMRFPDIDPGYERVRHKNYGKPMLDDGIHLNKLKLLKISAGFRF"
     gene            complement(1998..2384)
                     /locus_tag="PLM04_08690"
     CDS             complement(1998..2384)
                     /locus_tag="PLM04_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03183.1"
                     /translation="MTFTETGPKEYLCTNPQTPHETTCPPANPPLTKRDDSNANVTSG
                     VGRLWQSAVSFFKNNVISHLFGEKTLAKRSYSDQDSCVANGCDWIPNAQSNYPPYEFT
                     GKCECPGICYVIPADCGFKTPWFIRD"
BASE COUNT          789 a          515 c          417 g          721 t
ORIGIN      
        1 tgcggattgg tacataaata ttccttcggt ccggtttcgg taaatgtcac cacttgcgat
       61 ggcgccaaaa tccatgccct atccctttga acgctgtaat tattattcca gaaatattgc
      121 gatccatcgg agttgatttc ggcccttgcc ggcggaggat ttatttttct gtttgatatt
      181 ctcggaattt tatcgctact catcatttgc gtataacaaa aattatcccg ccattcgcaa
      241 acttcattaa tgggcatatt aatttgaggt aataaagccg tactgataca ttcgctttgg
      301 ttgcaaccgc cgcaatagga ttttttacac cagtcagata aattagaact cggcgtcgga
      361 gtcggagttg ggaaaggcgt tattgtttca ggcggtaaag taaaagttgg tgctggcgtc
      421 ctggttccac ttggcgttgc agttggcgtt cgcgttggcg aaacgtttgg acaaatacaa
      481 cgattattaa catcatccca gtcacagcca ttgactaaac agacctcctc atcaccataa
      541 ctttcaatgg ctaaggtact ccgacttaaa aaagataata aagcaaaaat agcgacaaaa
      601 gataaaatag agataaaaat aaaagatttt gttttaggca taaaacaaga actaaaccga
      661 cttcaaggtt attatacaaa aaaataacca atcaagcaac ttatccacaa ataaaaaagg
      721 accgctaaaa agcagtcctc tattttatta aaaaaataaa tcattagaat cgaaaccctg
      781 cagaaatttt caagagcttc agcttgttca aatgaattcc atcatcaagc attggtttgc
      841 catagttttt atgacgaact ctttcatagc cgggatcaat atcaggaaat ctcatttttt
      901 gagaattgtt attatagagc aacagtcctt tgtaaagatg ttcgctgtcg tcatttgtaa
      961 cagaaaaatt ctttccactt cccaattgta actctacatt gaaaaatata ttattcccaa
     1021 agtctaatcc tactactgga gcaagactaa attctgtttt gttttgtttg atctgccaag
     1081 atatatcgcc aaaataattc ggcttctgat ggtacataaa tacgtaatca tacgggtaca
     1141 agaaattgag cgtccaatca gattgaactt tagctttgct cataaacaat gctacaccaa
     1201 atcgaggaaa aatcgagaag tttctggaaa taggaatttc aaatttcaga gatggattaa
     1261 taccaaaatt ccataagtcc attaccattt caacttttgg atgttccatc acagtatcaa
     1321 gataccaatc agtatcttga tagtgtccat ctggagcagg aagttcataa tatggtcccc
     1381 acacttgttt gacatataga atgtatccct cttcttgaac ctttaactga tcctgtcgtg
     1441 caaaaacatc aaccgcaaag aaaacgcctt tcgtaatacg caaatcaata cctaaattaa
     1501 tttgcggaat aacattcttg tagtcacttc tggaataacc gttataaact gttctatcac
     1561 ccggtttaaa ataaaaatcc ggaacttcgc cattcaatac aaaataataa taatcagtag
     1621 gagcgggagc catttgcggt cggcattgaa accatctctc acagaaccac acttcattta
     1681 aaggatcgag aggccaagaa taacctgttt gaccaacata aacagtcgta tacccaagat
     1741 cccaactcgg cctgaccgag tcagtaccca gcggcaatcc ataacccaca gaaaacctga
     1801 aataaacttt cttttctttt ggattatcgc cagcccaagc cagcgaaaat cccagcaaca
     1861 ttacaatcag aaacagcaaa aatcttttct ttttcttttt catgacacac ctcctctgcc
     1921 gtctcgtttg gatttgcttc gcctttcagc aaaacccgag acgcgcagat ctttatttca
     1981 ttattaaaga tctactccta atctctaata aaccacggcg ttttgaaacc acaatccgcc
     2041 ggaataacat aacagattcc cggacattcg cactttcctg taaattcgta aggaggataa
     2101 ttggactggg cattaggaat ccaatcacaa ccattggcaa cacaggaatc ttgatcagaa
     2161 taagatcttt ttgctaaagt tttttcgccg aataaatgtg aaatcacgtt attcttaaaa
     2221 aatgatacgg ctgattgcca caatcgtccc actcctgaag taacattcgc attcgaatca
     2281 tctctttttg ttaaaggtgg attagctggt ggacaagttg tttcatgcgg tgtttgcgga
     2341 ttggtacata aatattcctt cggtccggtt tcggtaaatg tcaccacttt tattattatt
     2401 gaaaaaattt aagccttatt ttaaggaaaa acccgaattg gt
//
LOCUS       DAOTAX010000143         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_33834_length_2404_cov_33.206045, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000143 DAOTAX010000000
VERSION     DAOTAX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33834_length_2404_cov_33.206045"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..2335)
                     /locus_tag="PLM04_08695"
     CDS             complement(182..2335)
                     /locus_tag="PLM04_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HON03184.1"
                     /translation="MRQLIFSILILVLSNNLFGQHSQPDTLIAMPFVGQIKLDGELNE
                     PCWSDAVPIENFTQREQNEGMSATEKTRIAVVYNKNEMYIGIWCFDSEPNKISAQQMS
                     RDFSWGSDDNIEIMISPFNDNRNGYLFVTNPNGALADIWVGDEGKDFNKDWNGVWDVA
                     VQKNEQGWFAEMVIPFSTLKFTKDSTQVWGINFERNIRRKKEQLMWQGWSRLYDIEKI
                     SQGGKISGLHNISQGTKIEIKPYLLAGTQLSSDKPTNTAKIGGEVNFDITPTFKLNFT
                     ANTDFAQVESDRKQINLSRFSLYYPEKRQFFLEGKNYYDLSIGSVRLFYSRKIGIDQN
                     SVVPIIGGARLFGKLNQTNIGVMSLQTYAKDSIPTTNYSIIRVKQDIFKQSSIGLIST
                     QKYSDKGYNRVYGADFTYSTSEIFDNKNLIIGGEFALSETKVFANNDNLNKDNLSYNV
                     FLSYPNDVIEYDCGFTTVEKGFNPEMGFTNRNNYQMLYTELQFNPRFKKLPFFRNLIF
                     KPIDINYYINEETKETESVFYEWRPFGFESKSGEFMEFNVQHIFDKPTEDFELIDSIY
                     IPAGEYWDNRFEIQASTFRGRKISAETAVNVGEFYTGHRQEFSLATFFNFNKHLNVSL
                     DWQRNYISLPEKTFTTDEIGGRVDYAFNPKLQTSLFAQWNNEDNNILLNYRINWIPKI
                     GSFFYFIINQEYNTENTIKLERTTIIGKLIWRFAI"
BASE COUNT          696 a          451 c          402 g          855 t
ORIGIN      
        1 aaatcgttgg ggattgcggg ctacaaacct gtccagttac accgaacttg atgcggggaa
       61 gaacgctaga ataaccactt aaaccccaat gttttatacc gcttgttatg cactggcggt
      121 tcttgttttt atctgtatat ctgtcaatta tgcaattttt aataaacata atcttactgt
      181 tttaaattgc aaaacgccat atgagttttc cgataatagt tgttcgttcc aatttgattg
      241 tattttcagt attatattct tgattgatta taaaatagaa gaaactccca atttttggta
      301 tccagttaat gcgatagttc agcaagatat tattgtcttc attattccat tgggcgaaca
      361 gactggtttg tagtttagga ttgaaagcat aatcaacacg tccgccaatt tcatcagttg
      421 taaaggtttt ctcgggtaaa cttatgtagt ttcgctgcca atccagactt acattaagat
      481 gtttattgaa attgaaaaaa gtagcgagtg aaaattcctg cctgtgtccg gtatagaatt
      541 caccaacatt aacagccgtt tctgcagata tttttcgtcc tctgaaagtg cttgcttgaa
      601 tctcaaaacg gttatcccag tattcacctg ctggaatata aatactgtca atcagttcaa
      661 aatcttcagt aggtttatcg aatatatgtt gtacgttaaa ctccataaat tctccacttt
      721 ttgattcgaa accgaatgga cgccattcat aaaaaactga ctctgtttct tttgtttctt
      781 cattgatata atagttaata tcaattggtt tgaagattaa atttctgaaa aacggaagtt
      841 ttttaaagcg cggattgaat tgaagttcgg tataaagcat ttgatagttg tttcgattag
      901 taaaacccat ttccggatta aacccttttt caaccgttgt gaacccacaa tcatattcaa
      961 ttacatcatt tgggtaactt aaaaaaacat tataggaaag gttgtctttg tttaaattgt
     1021 cattattagc aaaaactttg gtttcagaca aagcaaattc tccgcctata atcaggtttt
     1081 tattatcgaa aatctcggat gtggaatatg tgaagtccgc accataaaca cggttatatc
     1141 ctttgtcgga atatttttgc gtgcttatta aaccaatact tgattgtttg aaaatatctt
     1201 gttttacacg aattattgaa tagttggttg taggaataga atcttttgcg taggtctgta
     1261 atgacattac acctatattg gtctggttta atttaccaaa aaggcgcgct cccccaatta
     1321 ttggaactac gctattttgg tcaattccta tttttcggct atagaaaagc cttacgcttc
     1381 caatgcttaa atcgtaatag tttttccctt ccagaaagaa ttgtcgtttt tcggggtagt
     1441 aaagagaaaa tctcgacaga tttatttgtt tcctgtccga ttcaacttgt gcaaaatcgg
     1501 tgttggcagt gaagtttaat ttgaaagtag gggtaatatc aaaattaacc tcacctccaa
     1561 ttttagcagt attagtaggt ttatcgctgc ttagttgagt gcctgcaagt aggtatggct
     1621 ttatttcaat ttttgtccct tgactaatat tatgaagtcc tgaaattttc cccccttgag
     1681 atattttttc tatatcgtac aatcttgacc atccttgcca catcaattgc tcttttttcc
     1741 ttcggatgtt cctttcaaaa tttatacccc aaacctgtgt gctgtctttg gtgaatttca
     1801 gcgtagaaaa aggaataacc atttcggcaa accaaccttg ttcgtttttt tgcacggcta
     1861 catcccatac gccgttccag tctttattaa aatctttgcc ctcatcgccc acccaaatat
     1921 cagccaaagc cccgttggga tttgttacaa ataagtaacc gtttcgattg tcattgaaag
     1981 ggctaatcat aatttctata ttatcatcac ttccccaact aaaatcacgg ctcatttgct
     2041 gtgctgaaat tttattgggt tcactatcaa aacaccaaat tccgatatac atttcgtttt
     2101 tattataaac aacagcaatc cgtgtttttt cggttgcaga cattccttca ttttgttccc
     2161 tttgagtaaa attttcaatc ggaactgcat cagaccagca aggttcattt aattctccgt
     2221 caagtttaat ttgaccaaca aaaggcattg caataagtgt atcgggctgt gaatgttgtc
     2281 caaacaggtt attgctcaat accaaaatta aaatactgaa aataagttgt ctcatgatct
     2341 aatatagtct atttcccgat tcattgtcat ttagctgtgt cttttctccg cttgtgcata
     2401 acgt
//
LOCUS       DAOTAX010000144         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_33895_length_2401_cov_27.130861, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000144 DAOTAX010000000
VERSION     DAOTAX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33895_length_2401_cov_27.130861"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLM04_08700"
     CDS             complement(<1..227)
                     /locus_tag="PLM04_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03185.1"
                     /translation="MIKAKRVSIFFLIFIFLYPISLSAKNPLKEKWVQTAIQRSEEQL
                     SAAIVQFRDSNKTPRTFENGKIVLVSYKDWT"
     gene            complement(258..>2401)
                     /locus_tag="PLM04_08705"
     CDS             complement(258..>2401)
                     /locus_tag="PLM04_08705"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase 6 family protein"
                     /protein_id="HON03186.1"
                     /translation="ESCLRMSGNWLYVSGLVFRNGHTPRKTVIEFRTGSKEYAFNSVL
                     AECVVDQFNQKVKDSTDHWIALYGQKNTIRNCYFAGKTNLGTTLIVCPDDENSINNQH
                     LIYRNYFGFRPRLGSNGGETIRIGTSEVCTNSSETIVKGNYFEHCSGETEIISNKSTD
                     NSFLNNTFYECEGSLVLRHGNNATVAGNWFIGNGKAFTGGIRVINEGHKIFNNYFYRL
                     AGDDFRSSLAIMNGIPNSPANGYAPVKNVIVANNTYYDCTSPWSFGVGFGFRNRIAKP
                     ENTLLLNNLVYCPNTNELIKYYDQTDGIMLDNNILVNSKGNMEMKGTVKGEIFQSKMN
                     DLEIVYTNTAAKRLAFIKEDILGQVRTTPVIGAFQNNIVNPTIEMATKHNCGPEWYRN
                     KITETTKTEKRFGKATVVEAGLDLLSKAVKNAGDNDTLLLMPGEHVVTKKMAINQNLV
                     IRSADNKVKPIIIMKADKENAYMFEISNNVKLQLEGVKIDGNSESNFPTKYAFISSRE
                     GAVHFSLIVNQCEIFGFNVKTGSIYRAYKGSYADSIVVTNTVMKDCFRGFSLSEEKDD
                     KGTYNAEIVKFENSVFDNFTQHALDYYRGGNDESTLGGFLFVNHCVFNNIGQDEKQSM
                     LKLNGIVNVSIANSIFSNSMAKTSVKLSGNKNIIQNCCFYNCPAPKVENGAISQNLYF
                     DNPRFEKKSFLLSKKSPLIGKATDGGNIGLR"
BASE COUNT          715 a          467 c          393 g          826 t
ORIGIN      
        1 cttgtccagt ctttgtagga cactaaaact attttcccgt tttcgaatgt tctgggtgtt
       61 ttattggaat ccctgaattg tacaatagca gccgacaatt gttcttcgga tctttgaatt
      121 gcggtttgca cccatttctc tttcagcgga tttttcgctg aaagagaaat gggatacaga
      181 aaaatgaata ttaagaaaaa aatcgaaact cgtttagctt ttatcataat gaatatttct
      241 cctgaaataa caaaaattta tctcaaacca atatttcctc cgtcagtagc tttccctatt
      301 aaaggagatt tctttgaaag gagaaaactt tttttctcaa atcggggatt gtcaaaataa
      361 agattttgtg aaatagctcc gttttctact tttggtgccg gacaattata aaaacagcag
      421 ttctgaatta tgtttttatt acctgaaagt ttgacacttg tctttgccat tgagttggaa
      481 aaaatagaat tggcaatgga aacattcaca ataccgttta gctttagcat ggactgtttt
      541 tcgtcttggc cgatattgtt aaaaacacaa tgatttacaa aaagaaaacc tcccaaagta
      601 gattcgtcat tccctcccct gtaatagtcc aaagcgtgtt gggtaaaatt gtcgaaaacc
      661 gaattttcaa attttactat ttctgcattg tatgtccctt tatcatcttt ttcttcgctc
      721 agactaaaac cacgaaaaca gtctttcatc actgtattgg ttaccacaat agagtcagca
      781 tacgaacctt tgtaagcgcg ataaatgcta ccagttttta cgttaaaacc gaaaatttcg
      841 cattgattca ctattaagga aaaatgcaca gcaccttcac gagaagaaat aaaggcgtat
      901 ttggttggga aattcgattc actgttacca tcaatcttaa caccttcaag ttgtagcttt
      961 acattatttg agatttcgaa catgtaagca ttttccttgt cggctttcat aattatgatt
     1021 ggttttacct tattgtcagc tgagcgaata accaaatttt ggttaatagc catttttttt
     1081 gtaactacat gttctcctgg catcagtaac aaggtatcgt tatcgccggc attttttaca
     1141 gctttggaaa gtaaatcaag tcctgcttct acaactgtgg ctttcccaaa acgtttttct
     1201 gtttttgtag tttcggttat cttatttcta taccattcag gaccacaatt atgttttgtt
     1261 gccatttcta tagtcggatt tacgatattg ttctgaaaag cgccgataac aggagttgtt
     1321 ctaacttgtc ccaaaatgtc ttctttaata aaagcaagtc ttttagctgc tgtattggta
     1381 tatacaatct ctaaatcgtt catttttgat tgaaaaattt caccttttac tgttcctttc
     1441 atttccatgt ttcccttgct attcaccaaa atattgttgt ccaacatgat accgtccgtc
     1501 tggtcgtaat atttaatcaa ttcgtttgta tttgggcaat aaaccaaatt attcaacaac
     1561 aaggtatttt caggttttgc aatacggttt cgaaaaccaa atcctactcc aaatgaccaa
     1621 ggggatgtac aatcataata cgtattattg gcaactatta cattttttac aggagcataa
     1681 ccattggctg gtgaattagg aatgccattc ataatagcca aactcgaacg gaaatcatct
     1741 ccagccaaac gataaaaata attgttgaaa attttatgtc cttcattgat aacccttatt
     1801 cctccggtaa aagctttacc attaccaata aaccaattac ctgctactgt agcattgttc
     1861 ccgtgacgaa gcactaaact cccctcacac tcgtaaaaag tattattaag gaatgaatta
     1921 tctgtagact tgttggatat aatttccgtt tctccactgc aatgttcaaa ataattccct
     1981 tttactattg tctctgaact gttggtgcaa acttcactgg taccaatacg aatggtttct
     2041 ccaccattgg aaccgagacg tggccgaaaa ccaaagtaat ttctataaat caaatgttga
     2101 ttgttgatgg agttttcgtc atcgggacaa acgattaaag tggttcccag atttgttttg
     2161 ccggcaaaat aacaatttct tatcgtgttt ttttgtccat aaagggcaat ccaatggtcg
     2221 gttgaatctt tcactttttg gttgaattgg tcaacgacac attcggctaa aactgaatta
     2281 aaggcatatt ctttggagcc ggttctaaac tcaattaccg tctttcttgg agtgtgtcca
     2341 tttcgaaata caagaccgga aacatacaac cagtttcccg acattcttaa gcacgattct
     2401 c
//
LOCUS       DAOTAX010000145         2356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_34720_length_2356_cov_22.375489, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000145 DAOTAX010000000
VERSION     DAOTAX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34720_length_2356_cov_22.375489"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1092
                     /locus_tag="PLM04_08710"
     CDS             118..1092
                     /locus_tag="PLM04_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009230531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HON03187.1"
                     /translation="MEKIKFIDLFCGIGGFRIAMEEACRENDLIPECVFSSDIDTFCQ
                     DSYEANFGHRPTGDITKVDEKDIPDHDILFAGFPCQPFSIIGQMQGFNDIRGTLFFDI
                     ARILKHKRPKAFVLENVKQLVGHDKGRTLKIIIKTLQELGYHVQYAVLNALDYGLPQK
                     RERVIIVGHREPIMFSFPSPVRPFKPLSEILEKKVDKKYYASEYIVNKRKAKHKSAYK
                     LSIWHENKAGNICSYPYSCALRAGASYNYLLVNGERRLTPREMFRLQGFPDTYKIVVN
                     DSQARKQAGNAVPVNLVKAVILKLLPYVASSLDMTSVLREYEVEYGKG"
     gene            1079..1831
                     /locus_tag="PLM04_08715"
     CDS             1079..1831
                     /locus_tag="PLM04_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03188.1"
                     /translation="MAKDKSPKLRTVNKSVSAFPLNEFPKDFPFLLGKELVYLLASKG
                     KAELEGSEWENIFANCIGADWKPSNVGLDDVVMGNTAWGAKTVKSSKPSNQKKVRLIS
                     GRNSPVYSFGERIDTSADPNLIGKLVLDIWNERVSAIREKFKHLRTVVLIKSNDLSEV
                     VVFEFETIRYDLELYKWEWNKNNNLIGIDKKTEEHRFTWQPHGSQFTIIEEVPEKSLV
                     IRIKQPKTLDKEQILKALGFDKSWITVIQKNG"
     gene            1824..2276
                     /locus_tag="PLM04_08720"
     CDS             1824..2276
                     /locus_tag="PLM04_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HON03189.1"
                     /translation="MADVFSKEQRSAVMRQVKSSRNKSTELKLIDFFKTNNIKGWRRN
                     YKLFGKPDFTFPTLRTVVFVDGCFWHGHNCRNTKPQVNKEYWNKKIERNKQRDKEVTL
                     TLKQKDWTVIRLWECELKNEKLLTKRLKEKLPTTAPIRKWGFCASNEK"
BASE COUNT          835 a          407 c          474 g          640 t
ORIGIN      
        1 gtaggcaatt ttattagagc ggacaccact aacaaaaacg gttgaaaaaa tgtgtacttt
       61 aacgacttcg caaaccgaca aaaaacttaa atttggcgaa atcttaattg tacgacaatg
      121 gaaaaaataa aatttattga cctattttgt ggcattggcg gttttcggat agctatggaa
      181 gaagcgtgca gggaaaacga cctcattcct gaatgtgttt tttcatcgga catagacaca
      241 ttttgtcaag atagttatga agccaacttc ggacatcggc ctacaggcga catcacaaaa
      301 gttgacgaga aagatattcc tgaccacgat attttgttcg caggattccc gtgtcaaccg
      361 ttcagtatta taggacagat gcaaggtttc aatgacatac gtggaacttt gttttttgac
      421 attgcaagaa ttctcaaaca caaacgtcct aaggcatttg tgctagaaaa cgtaaagcaa
      481 cttgttggac acgacaaggg tagaacttta aagataatta ttaaaacttt acaagaactt
      541 ggttatcatg ttcagtatgc agttttaaat gcactagact acggactacc acaaaaacgt
      601 gaaagagtaa taattgttgg acaccgagaa cctattatgt tttcatttcc atcaccagta
      661 agaccattca aaccgctttc tgaaatactt gaaaagaaag ttgacaagaa atattacgca
      721 tcggaataca tagtaaacaa gcgtaaagca aagcataaat cagcatacaa actttctatt
      781 tggcacgaga acaaagcagg taacatttgc agttatccct attcgtgtgc attgagagca
      841 ggtgcatctt acaactattt gctagtgaac ggtgagagaa gattaacacc aagagaaatg
      901 tttcgtttgc aaggttttcc agatacttac aaaattgttg tgaatgacag ccaggcaaga
      961 aaacaagccg gcaatgcagt tcctgtgaat ttagttaaag cagttattct gaaactatta
     1021 ccgtatgttg cctcctcatt ggatatgact tcggtattaa gagaatacga agtagaatat
     1081 ggcaaaggat aaatcaccga aacttcggac agtaaacaaa tctgtttcag catttccgct
     1141 aaatgaattt ccaaaagact tcccatttct tttaggaaaa gagttggttt atcttttggc
     1201 ttcaaaaggg aaagctgaat tagaaggttc tgaatgggag aatattttcg ccaattgcat
     1261 tggtgcagat tggaaaccat caaatgttgg gttagatgat gttgtgatgg gtaacacagc
     1321 ttggggtgca aaaactgtaa aatcatcaaa gccctcaaat caaaagaaag taagactaat
     1381 ttcggggcgt aactctcctg tttattcgtt cggtgagaga attgatacat cggcagaccc
     1441 aaatttaatc ggcaaacttg ttttagatat ttggaacgag agagtttctg caatcagaga
     1501 gaaatttaaa catttgcgta cagttgtttt aatcaaatca aatgaccttt cagaagtagt
     1561 agttttcgag tttgaaacta ttcgctatga cctcgaactt tataaatggg aatggaacaa
     1621 aaataacaat ctgattggca ttgacaagaa aacggaagag catcgtttta cttggcaacc
     1681 gcacggttca cagtttacaa tcattgaaga agttcctgaa aaaagtttgg taattcgtat
     1741 caagcaaccg aaaacactcg acaaagaaca gattttaaaa gcgcttggat ttgataaatc
     1801 ttggataact gtaatccaaa aaaatggctg atgttttttc caaagaacag cgtagcgcag
     1861 taatgcgaca agtaaaatca agtcgcaaca aatccaccga gttaaaactt attgactttt
     1921 tcaaaactaa caatatcaaa gggtggcgaa ggaattacaa actctttggc aaaccagatt
     1981 ttacatttcc gactttgaga acagttgttt ttgttgatgg ctgtttttgg cacggacaca
     2041 attgccgaaa tacaaaacct caagtcaaca aagagtattg gaataaaaaa attgagagaa
     2101 acaaacaacg agacaaagaa gtaacactga ctttaaaaca aaaagattgg actgttattc
     2161 gactttggga atgcgaactg aaaaacgaaa agctattgac caaacgactg aaagaaaaac
     2221 tgcctacaac agcacctata cgcaagtggg ggttctgtgc ttcgaatgaa aaataagtgc
     2281 taaatatgaa gagtagttct tcgtaggaaa gtttggttcg agaaagcccc acctgcgtat
     2341 agctgcaaac cgttgt
//
LOCUS       DAOTAX010000146         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_35901_length_2292_cov_58.085382, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000146 DAOTAX010000000
VERSION     DAOTAX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35901_length_2292_cov_58.085382"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1841)
                     /locus_tag="PLM04_08725"
     CDS             complement(138..1841)
                     /locus_tag="PLM04_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HON03190.1"
                     /translation="MEASTTVNKMLAGNKIFVPTYQRAYSWDTEFDKSKTQKHVNVFL
                     TDLEDYNKSLASTPYYFGHFLFEEKSESHFGVIDGQQRLTTIVIFLSALFSKLKSIRT
                     LTENEEVLFENMIKRKSNYIFSTVDYDNQLFKDYIVDQTKKNKNGLETESARRIVNAF
                     DFFTKALKDKEEVYLTKMLKTVNEASCSTHPVKNESEAIQMFIFQNNRGKKPSNLEII
                     KAQFMFNVHLYGGEEKDSLIEEIKNRFEKIYKSISSIEYYINEDDVLVYTLRVHFNSL
                     WETNAIEKINKLLSDGNPIPFIKSFTHSLAISFEHLTTFFGKDEREQLEVHSLITLGG
                     IGIAIPFVIKAYKFDLPTDTINQLCSKLESIVLRHRLIGTRAELTSRINDVYQKFTTE
                     QPDIKPIVDRIEWMKNVSNDSWWWSYWNRSEFEKSIQGGMHHSIAKFLLWKYENYLEN
                     QGKNGYSLTRFDKIVSPELEHIAPQTPTDGEPIAAGYCEYDEEFRNQYIDCLGNYLLL
                     SKSHNCSVGNKPFSEKRNSYNHLEQQREIQKMTAESEIWDKEKISLRKDKIVKFILEN
                     F"
BASE COUNT          665 a          393 c          347 g          887 t
ORIGIN      
        1 tgaggctata tgcagtaagg gattgcgggc tactttcctg tccacacgca ccgaagtttg
       61 agcggggcag aacgcttgaa ttaccactga aacccttatt gcatatagcc tttgttagag
      121 ccatgtgcat tattttctta gaagttttca agtataaatt tcacaatttt gtctttacgc
      181 agactgattt tttctttgtc ccaaatttcc gattccgctg tcattttttg aatttctctt
      241 tgttgttcaa gatggttata cgaatttctt ttctcgctaa atggtttgtt tccaaccgaa
      301 caattgtgtg atttagatag aagtaaataa tttcctaaac agtcaatata ttggtttcta
      361 aactcttcat catattcaca ataacctgct gcaattggtt ctccgtctgt cggtgtttgt
      421 ggtgcaatgt gttctaattc agggctaaca attttgtcaa aacgagtcaa tgaatatcca
      481 ttttttcctt gattttcaag ataattttca tacttccaaa gcaagaattt tgctattgaa
      541 tggtgcattc cgccttgaat tgatttttca aactcacttc tattccaata tgaccaccac
      601 caagaatcat ttgaaacatt tttcatccat tcaattctgt caactatcgg tttaatgtca
      661 ggttgttctg ttgtaaattt ttgatataca tcattaattc ttgatgttaa ttctgctctt
      721 gttccaatta agcgatgtcg taaaacaatt gattcgagtt tactgcaaag ttgatttatc
      781 gtatctgtcg gcaaatcgaa tttgtaagcc ttgattacaa aaggaattgc gattccaatt
      841 ccacctaatg ttattagtga atgaacttct aattgctctc tttcgtcttt gccgaagaat
      901 gttgttaaat gctcaaaact gattgctaaa gaatgtgtga atgactttat aaatggaatt
      961 ggatttccgt ctgaaagcag tttgtttatt ttttcaattg catttgtttc ccaaagtgaa
     1021 ttgaaatgaa ctctcaaagt gtaaaccaaa acatcatctt cgttgatata gtattctatt
     1081 gatgaaattg atttgtagat tttttcaaag cgatttttta tttcttcaat caaactgtct
     1141 ttttcttcac caccataaag atgaacgtta aacatgaatt gtgcttttat gatttccaaa
     1201 tttgatggtt ttttcccacg gttgttttgg aaaataaaca tttgaattgc ttcggattca
     1261 tttttaactg gatgtgtcga acatgatgct tcatttacag ttttcagcat tttcgtcaaa
     1321 tatacttctt ctttgtcttt taatgctttt gtgaaaaagt cgaacgcatt aactattcgt
     1381 ctcgctgatt cggtttccaa accattttta ttttttttag tttggtcaac aatgtagtct
     1441 ttaaataact gattgtcata atcaacggtt gaaaaaatgt aatttgattt acgcttaatc
     1501 atattttcaa aaagcacttc ttcattttca gttaaagtcc gaattgattt caattttgaa
     1561 aataaagcgg aaaggaaaat aacaatagtt gttagtcttt gctgtccgtc tattactcca
     1621 aaatggcttt ctgatttttc ttcaaacaga aaatgaccaa aataatatgg tgttgatgcc
     1681 aaacttttat tataatcttc taaatctgtc aaaaaaacat ttacgtgttt ttgagtttta
     1741 cttttgtcaa attctgtatc ccaagaataa gcacgctgat aagtggggac aaaaatctta
     1801 ttccctgcta acattttatt taccgttgtt gatgcttcca ttatatcaaa ttataaaatt
     1861 tatactttaa atttaaaaga agtaacggca tagccgttac ttctttttgt tcattagatt
     1921 gcaggaacat ggtattttcc attaggattt ttactacatg tcgaagacgt taaacttgaa
     1981 atacttgaac gctttgttcc acagtattta caagtatatt cctttttttc tgaaccttca
     2041 tacaacgcat gatatttacc actagggttt ttactgcaag ttgaagatgt taaacttgaa
     2101 acacttgaac gttctgttcc gcaatacata caaaaaaact tagccataac aattatattt
     2161 aatttttgcc tactctgatt agttttcggc ttccctattt tcaaatttca aaaatatgtc
     2221 gagcctacgt caaaatatgt cgttattcca aaaatctgtc tgcgtgtctt tttttcttgc
     2281 attggctcta ac
//
LOCUS       DAOTAX010000147         2283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_36075_length_2283_cov_39.918312, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000147 DAOTAX010000000
VERSION     DAOTAX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36075_length_2283_cov_39.918312"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1334)
                     /gene="metK"
                     /locus_tag="PLM04_08730"
     CDS             complement(54..1334)
                     /gene="metK"
                     /locus_tag="PLM04_08730"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HON03191.1"
                     /translation="MAYLFSSESVSEGHPDKIADQISDALLDNFLALDRDSKVACETL
                     VTTGQVIIAGEVNTTAYVDVQSVARRVINRIGYTKSDYMFDGNACGILSALHDQSPDI
                     QRGVEREDPIEQGAGDQGMMFGYACNETSNYMPVALDLAHALVGELAKIRKEGKQMTY
                     LRPDAKSQVTIEYDDSNRPLRVHTIVISTQHDEFIQPSLTKSQDEADREMLLIIRQDV
                     QNILIPRVKLLDKQFAELFKDNFTLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEVLVQVAYAIGVADPINLYID
                     TFGTSKVKFSDSEIAEKINNIFDMRPKAIEERLKLRYPIYEETASYGHMGRTPMEVRK
                     VFKDGNGNQITKTVELFTWEKLDKVKEIKSIFQL"
     gene            1988..2269
                     /locus_tag="PLM04_08735"
     CDS             1988..2269
                     /locus_tag="PLM04_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HON03192.1"
                     /translation="MNSLQIVGSLASLLSILNQFPQAIKVFKTKDTHSISLTMYCIVV
                     VCITLWLVYGIMLKDGPLIWANGLSLFPITYIFIVKLTNTIKGKDKLSI"
BASE COUNT          702 a          464 c          358 g          759 t
ORIGIN      
        1 attttgaatc ttaaaaattt acacagttta tgaaatattc tccattatac tttttaaagc
       61 tgaaaaatcg actttatttc tttcaccttg tcaagttttt cccatgtaaa taattcaact
      121 gtttttgtaa tttggtttcc atttccatct ttaaaaacct ttctcacttc cattggtgtt
      181 ctgcccatgt gtccatacga agctgtttct tcataaattg gatatctcaa ttttaatcgt
      241 tcctcgatgg ctttaggtcg catatcaaaa atattattta ttttttcggc aatttcacta
      301 tcagaaaatt ttacttttga agtgccaaaa gtatcaatgt aaagatttat cggatcagca
      361 acaccaatgg cataagctac ctgtaccaga acttcgtcgg cgacgccggc agctaccatg
      421 tttttagcta tgtgtcgagc ggcataagct gctgagcgat ccactttaga tggatcttta
      481 cccgaaaaag cgccaccacc atgtgctccc ttacctccat aggtatctac aattattttt
      541 cttcctgtca atccagtgtc gccatgagga cctccgataa caaattttcc cgtcggattg
      601 accagtaatg taaaattatc cttaaataat tcggcgaact gtttatcaag cagtttaacg
      661 cgaggaataa gaatgttttg cacatcctga cggataatca acaacatttc cctgtcggct
      721 tcgtcttgac ttttggtcag cgaaggttgt ataaactcat cgtgttgagt agaaataaca
      781 attgtatgaa ccctcaaagg gcggttgctg tcgtcgtact caatagttac ttgtgacttg
      841 gcatcgggac gaagataagt catttgctta ccttccttgc gtattttagc cagctctccc
      901 accaaagcat gtgctaagtc aagtgcaaca ggcatgtaat tggaagtttc attgcatgca
      961 tatccaaaca tcataccctg atcaccagct ccttgttcta tggggtcttc tcgttctact
     1021 cctctctgaa tatcaggcga ttgatcgtgt agtgcagata aaatgccaca agcatttcca
     1081 tcaaacatat aatcgctctt tgtataacca atccgattaa ttacacgacg cgcaaccgat
     1141 tgtacatcaa cataagctgt ggtatttact tctccagcta taattacctg accggtagta
     1201 accaatgttt cacaagccac tttagaatca cgatctaaag ccaaaaaatt atccaacaaa
     1261 gcatctgaaa tctgatcggc tattttatcg ggatgacctt ctgacaccga ttcagacgaa
     1321 aataaataag ccatatcatt aaattattaa attacttaaa aatcaaatgt ctgaatttac
     1381 tttgtaagtt gaagagaagg cagctaaaaa aggagaaaga aaattgaatc attttagcat
     1441 ttttttacgt ggttgcaagc agccaaatct ttccacttat aaataaattc tgtgcaaaat
     1501 taatcaaaat tctttgtttt tactaaattt gtacttatgt atatgacaaa aaaattgtat
     1561 aaacatttat gttttttatt ttcagatatt taataacggg gctttgcccc aaaacccact
     1621 tacttttttg tcttgaaaca aaaaagtaag caaaaaaatt aacttagttg aagcagctac
     1681 gctcaacata aatcaaataa atttgatttt gttttcgctt aaacgctgct ttcaagactg
     1741 tgtccacttc actcgaaaaa cttacgctcg aaaagctgaa atcgcccaaa ctcattccga
     1801 cgaaatacgt cggaactcaa acaaggacga tttataaagg cttttctcac ttttttcggc
     1861 tcaccggaca aggtcaaccc gaagtgtttg gcttatatgt gctgttttac agatatataa
     1921 aacaaatgtt tcaatttttg tcatgtgcta aaaaatttgt atctaaatat ttattaaact
     1981 tattcgtatg aattctcttc aaattgtagg ttctcttgct tctcttttgt cgatattaaa
     2041 ccaatttccg caagccataa aagtttttaa gactaaggac acccacagta tttcactaac
     2101 catgtattgc attgtggtag tatgtatcac attatggctg gtttatggga tcatgttaaa
     2161 agacggccca ttaatttggg caaatggact ttctcttttt cccatcactt acatttttat
     2221 agttaaactt accaacacca tcaaaggaaa agacaaactc tccatttgat tattgttgtt
     2281 ccg
//
LOCUS       DAOTAX010000148         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_36654_length_2255_cov_31.085000, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000148 DAOTAX010000000
VERSION     DAOTAX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36654_length_2255_cov_31.085000"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..223)
                     /locus_tag="PLM04_08740"
     CDS             complement(53..223)
                     /locus_tag="PLM04_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03193.1"
                     /translation="MKFQDMLRILLEKQRQWDSLTPEQQEAEKEKHVAYDECILSESG
                     YYDYELDEDEND"
     gene            complement(241..519)
                     /locus_tag="PLM04_08745"
     CDS             complement(241..519)
                     /locus_tag="PLM04_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03194.1"
                     /translation="MEWIIKQKEFERIFGGDMVQQNRFISSSKDVNVALDFAIRNQSL
                     MKKNEIQVVFEITSKNGRNISDISKLEIMFTSNTSFRIEKTETTDNDV"
     gene            complement(488..616)
                     /locus_tag="PLM04_08750"
     CDS             complement(488..616)
                     /locus_tag="PLM04_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03195.1"
                     /translation="MGKNTLTEFNQAAKTLIERGLEKLPVHNGMVNRGMDYKAKRI"
     gene            complement(625..807)
                     /locus_tag="PLM04_08755"
     CDS             complement(625..807)
                     /locus_tag="PLM04_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03196.1"
                     /translation="MHFDLSVRCSPDRKMLESYFRIKENYRDIAGVVRSYILLVPGSI
                     LELDPNQRKQVAIIGN"
     gene            1368..>2255
                     /locus_tag="PLM04_08760"
     CDS             1368..>2255
                     /locus_tag="PLM04_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HON03197.1"
                     /translation="MHKEIQITLPPEKVNNKSVLEKEIIKIINIEIQQITRWRIIRKS
                     IDARSYLPKFNLSVEVYWDEIPPEDTAYNFHYNFVGNKPSVLIIGSGPAGLFAALKLI
                     ELGLKPVILERGKQINQRKKDIASICRNECIDPNSNYCFGEGGAGTFSDGKLYTRSKK
                     RGDKLHVLQVFHHHGANENILYEAQPHIGSDKLPLIVENICKTIIDCGGEIHFETRIT
                     DILLKNGHITGCKTADNNIFEAASLILATGHSARDVYEMLYHQGVLLENKGFAMGVRV
                     EHPQQLIDSIQYHKKYRGDF"
BASE COUNT          720 a          411 c          384 g          740 t
ORIGIN      
        1 aattgaactc taattgagca ggtaggggga ttactccccc gccctactcg atttagtcgt
       61 tttcatcttc atcaagttca taatcatagt aacccgattc ggaaagtatg cattcatcat
      121 acgctacatg tttctctttt tcggcttctt gttgttccgg cgtaagcgaa tcccattgac
      181 gttgcttttc caaaagtatt cttagcatat cttgaaattt cataattctg tcaatttacc
      241 tcaaacatca ttatcggttg tttccgtttt ttcaatgcga aacgatgtgt tactggtaaa
      301 cattatttct aattttgaaa tgtcagatat gttgcgaccg tttttactcg ttatctcaaa
      361 tactacttgt atttcattct ttttcataag gctttgattt cttatggcaa aatccaaagc
      421 aacattaaca tccttgcttg aagatataaa tcggttttgt tgaaccatat ctccgccaaa
      481 tattcgttca aattcttttt gctttataat ccattccacg atttaccatt ccgttgtgaa
      541 caggcagttt ttccaagcca cgttctatta acgttttagc cgcttggtta aattcggtaa
      601 gcgtattttt tcccatctgc ttgttcaatt gccgataatt gcaacctgct ttcgttggtt
      661 ggggtccaat tcgagaatgg agcccggaac caacagaata taactgcgaa ctactcccgc
      721 tatgtcgcgg tagttttcct tgatacgaaa atagctctcg agcatttttc tatcgggact
      781 gcagcgaact gataaatcga aatgcattcc gcaatgttct gtaatgcaaa ttaaacaacc
      841 accaaaatcc cgggatacta caaatgtgtt ctcagaaaca aaaactattg aaatccaaaa
      901 tattacaatt tcaatcgact gaatttgact accaaactaa ccaaaatcag tcttttttta
      961 ctaaacttta acgttatgtt attcatgttt gcaaagttgg gtgaaatagt caattgttaa
     1021 acagcaagta atctgcattt ttttaaaaaa aattaatgtt gtccgataaa aataaaggag
     1081 caaatagtgt cgctcctacg gagctctaat gatatttgta acttattgac taccataata
     1141 tcgtcccgat gggacttaag agaaagctgc attgcagcga aattatggta gaaaaattca
     1201 acccacataa tctaagcccc attaggggcg atattatagt aagacatttt ctataatggt
     1261 tttcactaat ttttataaat gtttaccgaa caacaatgaa aaaaataatt ttttcttttg
     1321 agattaatcc tatacttttg tgataaagaa aaatttactt tataaatatg cataaggaaa
     1381 tccaaattac tttaccaccg gaaaaagtaa ataataaatc agtactcgaa aaagagataa
     1441 tcaaaattat aaatatagag atacagcaga ttacaagatg gagaattatt cgcaaatcca
     1501 ttgatgcccg ttcttatcta ccaaaattca acttgtctgt tgaagtttat tgggacgaaa
     1561 ttccaccgga agacacagcc tataattttc attataattt tgttggcaac aagccatcgg
     1621 tcttaattat cggttcgggg ccggcagggc tttttgcagc cttaaaactt atagaacttg
     1681 gccttaaacc tgttatattg gaaagaggca agcaaataaa tcaaagaaaa aaagatattg
     1741 cttccatttg tcgaaatgag tgtattgatc caaattccaa ttattgtttc ggagaaggag
     1801 gtgccggcac cttttctgat ggtaagcttt acactcgctc aaaaaaaaga ggagataaac
     1861 ttcatgtttt acaggttttt catcatcatg gcgcaaatga aaatattctt tatgaagcac
     1921 agccccacat tggttccgac aaattgccct taatcgttga gaatatatgt aagaccatta
     1981 ttgattgtgg aggagagatt cattttgaaa caagaattac cgatatttta ttaaaaaatg
     2041 gacatataac aggttgtaaa acggctgata ataatatttt tgaagctgca tccctgattt
     2101 tggctaccgg tcattctgcg cgcgatgttt atgaaatgct ttatcatcaa ggagtactgc
     2161 tcgaaaataa gggttttgca atgggtgttc gtgtggaaca cccccaacaa ttgattgata
     2221 gtattcagta tcataaaaag tatcgaggag atttt
//
LOCUS       DAOTAX010000149         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_37702_length_2206_cov_33.338447, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000149 DAOTAX010000000
VERSION     DAOTAX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37702_length_2206_cov_33.338447"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1930)
                     /locus_tag="PLM04_08765"
     CDS             complement(158..1930)
                     /locus_tag="PLM04_08765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HON03198.1"
                     /translation="MKNIKMTFKELNLKDELLSAIEELGFEQPMPIQERTIPFMQEKS
                     ADLVALAQTGTGKTAAFGLPILNMLDAAQKQTQALILAPTRELCIQISNDLKNYSKYL
                     KGIKVVAVYGGEDIRTQLRQLDTPPQIIVATPGRLIDLTERGKVSLQNIHYLVLDEAD
                     EMLNMGFKEDIETILKQTPENRRTMLFSATMPKEISNIVHQFMKDYEEIIVGQKNSGA
                     SNVEHIYYLTPAKYRYLTLKRIVDLNPDIYGIVFCRTRQETKEVAEKLMQDGYNADAL
                     HGDLSQAQRDNVMQKFRIRNVQLLVATDVAARGLDVSDLTHVINYNLPDDPEVYTHRS
                     GRTGRINKKGISISIIHSKEKHKIKELEKMLKKDFIQQQIPNGLEVCKKQLYHLINRM
                     EHVDVNEEQIQSYLPQIMKQLEYLSKEEIIKRFVSLEFNRFLDYYKDAEDLNEIHFYE
                     DRNNKSKNEKFSKKSGKRIRLKLNVGGREGIDTRRFLGIFNEVVGDKSINVGSIEITN
                     KFTFFDVYADQIDKVMNAFALEPQMDVCEVKGNKQFFEEKPSKFSNHSKKRETYPNIN
                     EGSGRKERTANSEKPWRQSQRTRN"
BASE COUNT          611 a          402 c          406 g          787 t
ORIGIN      
        1 aaacaaaaaa ttattttgca agacaaagga ggaaaaacat tccctcctta tgtcttgatg
       61 gaataaaggc tttactttgg tctctttctt ttaaaaaagt tacgaaaata aaacaaattt
      121 tgtaggtgat caggaattgt ggaaaaaaat caaaaattta attacgtgtc ctttgggatt
      181 gtcgccacgg tttttcggaa tttgcggttc tttctttcct tccggatcct tcatttatat
      241 tgggataagt ttcccttttc ttggaatgat tggagaattt cgaaggcttt tcttcaaaaa
      301 attgtttgtt tcctttcact tcacaaacgt ccatttgagg ctctaaagca aacgcattca
      361 ttactttatc tatctgatcg gcatagacat cgaaaaatgt aaacttattc gtaatttcta
      421 ttgaccctac attgatggat ttgtctccga ctacttcatt gaaaattccc aaaaaacggc
      481 gggtatcaat tccttcgcgt cctcccacat tcaatttcag acgaatacgc tttccgcttt
      541 tctttgaaaa tttttcgttt ttcgatttgt tatttctatc ttcgtaaaaa tgaatttcat
      601 tcagatcttc cgcatctttg taataatcga gaaaacggtt gaattccaat gaaacaaagc
      661 gtttgataat ttcctcctta ctcagatatt ccaattgctt cattatctga ggaaggtaag
      721 attgaatttg ttcttcgttt acatccacgt gttccattcg attaatcaaa tgataaagtt
      781 gttttttgca aacttccaaa ccattgggta tttgctgctg aataaaatct tttttcagca
      841 ttttttccag ttccttaatt ttatgttttt ctttcgaatg gatgatggaa atggaaatac
      901 cttttttgtt gattctgcct gttcttccac ttcggtgtgt gtacacttcg ggatcgtccg
      961 gcaaattgta gttgatgaca tgcgtgagat cgctcacgtc caatcctctg gcagcaacgt
     1021 cggtagccac cagcagctga acattgcgaa ttctgaattt ttgcatcaca ttgtcgcgtt
     1081 gtgcttgaga aagatctcca tgcaaagcat cggcattata accatcctgc ataagttttt
     1141 cggcaacttc ttttgtttcc tgacgggttc tgcaaaacac aatgccataa atgtcgggat
     1201 tgaggtctac aatccgtttt aaagtcaaat aacgatattt ggccggagtg aggtagtaaa
     1261 tatgttctac attggaagct ccggaatttt tttgtcccac tattatttct tcataatctt
     1321 tcatgaattg gtgaacgata ttcgaaattt ctttgggcat ggtggctgaa aaaagcatgg
     1381 tacgtctgtt ttcaggcgtt tgtttcagaa tggtttcaat atcttcttta aaacccatgt
     1441 tcagcatttc gtcagcttca tcgagcacca aataatggat gttttgaaga gaaacttttc
     1501 cgcgttcggt gaggtcgatt aatcggcccg gagtagctac aataatttga ggtggtgtat
     1561 caagctgtct taattgggta cgtatatctt ctccaccata aacagctaca actttgattc
     1621 ctttcaaata tttggagtaa tttttcagat cgttgctgat ttgaatacat aattctctgg
     1681 taggagccaa aatcagtgcc tgagtttgtt tttgagcagc atccagcatg ttgagaatag
     1741 gaaggccaaa agcagctgtt tttcctgtcc ctgtctgcgc caaagccacc aaatcggcag
     1801 atttttcctg cataaaaggg atggtacgtt cctgaatggg catgggttgc tcgaatccga
     1861 gctcttcaat agccgaaaga agctcgtctt ttaaattcaa ttctttaaat gtcattttaa
     1921 tgtttttaat acacgttttt gagcattcat ccgatgtaaa aattttattg aaagataaaa
     1981 cgtgtacttt gagatgcaga tttcaaaaat caaaaatata atgtcttgaa tttcggatat
     2041 ttactcgaaa cgtatagttg ttttttaatt cgggtgcaaa gatagtcatt tattttcata
     2101 tttgtatggt attgtcgatt ttgtttttgt tctatagttt gtcatgaaac gcacatgtat
     2161 tgcatacaca aacacgaatg aatataatat tcagacatgt gggttc
//
LOCUS       DAOTAX010000150         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_38178_length_2184_cov_24.263974, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000150 DAOTAX010000000
VERSION     DAOTAX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38178_length_2184_cov_24.263974"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLM04_08770"
     CDS             complement(<1..283)
                     /locus_tag="PLM04_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HON03199.1"
                     /translation="MLSQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVEL
                     KNNIKKYWWDSMVLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNK"
     gene            549..1433
                     /locus_tag="PLM04_08775"
     CDS             549..1433
                     /locus_tag="PLM04_08775"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HON03200.1"
                     /translation="MIKHSSCRFGLIKPSLIAGILIIVCFSINAQSSNQSEKALTTST
                     NSFGFSTINMLDSYLSPLKYSGSGFRYENDNRKFVSPENISLSKENHFKMNFGLTENP
                     AQTSEMWYLGMNFSWGMHHHFYVGENLLLLAGGLWDLDFMGKTNSRNQNNPFNIDLAT
                     NLNLSVEAVYRIRTPKRMLRLGFSMQAPFIGVMFVPEQGASYYEIFELQNFSNTLHFT
                     SLHNKLSLTENLSLDIPFNHTTLRFGFRNENLKFEANQMIFKRNDLSFHVGYIYDFYK
                     FEGTKNRAPSDFNSTYNY"
     gene            1449..>2184
                     /locus_tag="PLM04_08780"
     CDS             1449..>2184
                     /locus_tag="PLM04_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HON03201.1"
                     /translation="MKKHIILLSLLGMLFHSCMDEPVIQPNSYEGNFNALWEIIDTKY
                     CYLDYKNIDWDSIYTAYHQRIPYIKTDLDFFDFLGKMLEELKDGHVNLYSGFDVSRYW
                     KWYTDYPENFSSDIIYSDRYLGENYRIAGGMRYQKIDNGNIGYIYYGDFTSDFSDTNI
                     GYIFKYFSNCKGIILDVRNNGGGYMSGAEQLASYFFTEKTLTGYLCHKTGPGHTDFST
                     PIKLFTPANKNIQWLRPVVILTNRMTY"
BASE COUNT          698 a          412 c          362 g          712 t
ORIGIN      
        1 ctttgttgtc aatcagcgga tcattgaaag catccacatg tcccgaagct ttccacgttg
       61 tgggatgcat aaaaatagcc gcatcgatac ccactacatt ttcgtgaagc aagaccatac
      121 tgtcccacca atattttttg atattgtttt taagttctac accattttgt ccatagtcat
      181 aaacagcact caatccgtcg taaatttcgc tcgacggaaa aacaaaacca tattccttgc
      241 aatgagcaat cagttttttg aaaacatctt cttgtgaaag catatcaaca ttattttagt
      301 tatcattcct tcaaatcgta attcgatttt tttatccaaa acaaaacctt ttctcggtga
      361 aaaaaatatt ttcaagttta aaagattccc tgaaagaaat gcaaaattac agcttttttt
      421 atatttttgt tgattcaagc cgataaaaaa gtttattaat tcgctaaatt cattctgaaa
      481 attaacgaaa cagatattta tcaacgcaca tgatacaaaa tacaggtatt ttcctcaaac
      541 aaaaaaatat gattaaacac tcttcatgta gattcggctt aataaagcct tctctaattg
      601 ctggaatttt aattattgtc tgtttttcga taaatgcaca aagttcaaat caatccgaaa
      661 aagcgttgac aacttccact aacagctttg gattttctac catcaacatg ttggattcgt
      721 acctttcgcc attgaaatac agcggatcgg gatttcgtta cgaaaacgac aacagaaaat
      781 ttgtttctcc ggaaaatatc agtctttcaa aagaaaacca tttcaagatg aattttggtt
      841 tgacggaaaa ccctgctcaa acgagcgaaa tgtggtattt gggaatgaat ttttcttggg
      901 gaatgcatca tcatttttat gtaggtgaaa atctcttatt gcttgccgga ggtctttggg
      961 atctcgactt tatgggaaaa accaatagtc gcaatcagaa taatccgttc aatattgatc
     1021 ttgctaccaa cctgaatctt tcagtggaag ctgtttatcg tatcagaaca ccgaaacgca
     1081 tgctccgttt gggattttcc atgcaggcac cttttatcgg agtgatgttt gtacctgaac
     1141 agggagcgtc gtattatgaa atttttgaat tgcaaaattt ttctaataca ttgcatttca
     1201 cctcattaca caacaaactg tcgctcactg aaaatctatc gctggatatt cctttcaatc
     1261 acactacttt acgttttggt tttagaaacg aaaatttaaa gtttgaagcc aatcaaatga
     1321 tatttaaaag aaacgattta agttttcatg tcggatacat ttacgatttt tacaagtttg
     1381 aaggaacaaa aaatcgggcg ccttcggatt tcaacagcac ttataactac taatcaacaa
     1441 gttttttcat gaaaaaacat atcattttgc tttcactact cggaatgctt tttcattcgt
     1501 gtatggatga acctgttatt caacccaata gttatgaagg caattttaat gctttatggg
     1561 aaattatcga taccaaatac tgttatctcg attacaaaaa tattgactgg gattccatct
     1621 acactgctta tcatcaaagg attccgtaca taaaaacaga tttggatttt tttgattttc
     1681 ttggcaaaat gctggaagaa ttaaaggatg gacacgtaaa cctttattcc ggttttgacg
     1741 tcagccgtta ctggaaatgg tacaccgatt atcctgaaaa tttcagttcg gatattattt
     1801 attcagatcg ttatttaggt gaaaactata gaattgccgg cggaatgcgt tatcagaaaa
     1861 tcgataatgg aaatatcggg tacatttatt atggagattt caccagcgat ttcagcgata
     1921 ccaacattgg ctacatattc aaatattttt ccaattgcaa gggaattatt ctggacgtac
     1981 gaaacaatgg tggcggatac atgagtggag ccgaacaatt ggcttcctac ttttttactg
     2041 aaaaaacgct caccggttat ctttgccata aaaccggtcc ggggcatacc gatttttcta
     2101 cgcccatcaa attgtttact cctgccaaca aaaatattca atggttgaga ccggttgtga
     2161 ttcttaccaa ccgcatgacc taca
//
LOCUS       DAOTAX010000151         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_39692_length_2117_cov_30.751212, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000151 DAOTAX010000000
VERSION     DAOTAX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39692_length_2117_cov_30.751212"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLM04_08785"
     CDS             complement(<1..656)
                     /locus_tag="PLM04_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HON03202.1"
                     /translation="MVLNYIWIGFFLIAFVIACIQLLVFGNVQVFSDMLNSTFDSAKS
                     GFEISLGLTGVLSLWLGIMKVGERGGVIRLFSKAVAPFFEKIFPDLPKNHPAYGSMFM
                     NIAANMLGLDNAATPLGLKAMKEMQETNPVKDTASNPMIMFLVLNTSGLTLIPVTIMV
                     YRAQMGATNPSDIFLPILISTFISTVSGLFIVAAYQKINILDKTVLGTLAGLFIIVGS
                     "
     gene            complement(804..1994)
                     /locus_tag="PLM04_08790"
     CDS             complement(804..1994)
                     /locus_tag="PLM04_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HON03203.1"
                     /translation="MNRKKNKRIFGYPRNIFFTGLTSFLTDTSLKMVYSVMPMFLLSI
                     GASKTSLSLIEGVAESTASLIKTITGFWSDKIGKNKPFLLIGYGVSAVIIPLYSLVVS
                     PIQVLILRFVERVGKGIRTAPRDSIVAGSVENGETGKSFGLQKAMDNSGAILGPLIAF
                     ILLSAFKDNYRLIFLLAGIPSILGILVIIFGITEAKKNKESLVKKFRFGDFSKKFYFF
                     LAIVFIFTLGNSTDALLLVKANEIGIKVAFIPLVYLLTNVVSVLLSIPVGSLSDRVGK
                     EKILIVGYIIYALVYFGFGASSHIVVIASLFALYGVYSAATDGIQKAFVSDIVDENKK
                     GTGLGLYNALLGITLLPASLIAGILYDKVNSHVPFYFGAVTALIAAVLMFWFQRSFKK
                     QVVE"
BASE COUNT          715 a          421 c          377 g          604 t
ORIGIN      
        1 atactgccta caataataaa taaaccggcc aaagttccta aaacggtttt gtccagaatg
       61 ttgatttttt ggtaagcagc aacgataaat aaaccactta cagtggaaat gaatgttgaa
      121 attaggatgg gaagaaaaat atcggaaggg tttgttgctc ccatttgagc cctgtaaacc
      181 atgatggtta ccggtattaa tgtcaatcct gatgtattca gcaccagaaa cataatcatg
      241 ggattggaag cagtatcttt tacgggattg gtttcctgca tttctttcat ggcttttagc
      301 cctaatggag tagccgcatt gtcgagtccc aacatgttgg cggcaatatt catgaacatg
      361 ctgccataag cgggatgatt tttcggaaga tcaggaaaaa ttttttcaaa aaaaggcgcc
      421 acagccttag aaaacaatcg aattacgcca ccgcgttcgc caactttcat tatacccagc
      481 cacagcgaca atacgcctgt aagtcccaat gaaatttcaa aacctgactt ggccgaatcg
      541 aaagttgaat tgagcatgtc ggaaaaaacc tgaacatttc cgaaaaccaa cagttgaatg
      601 catgcaataa cgaatgcaat cagaaaaaaa cctatccaaa tgtaattcaa taccatcttt
      661 taaagttttt cgggcttaat gtggaaaacc ggatttttaa atattccgac aacttgcaaa
      721 attacaagat ttttccgatt tgcactcttt ttgacatgtg gagacagcat aaaaatgaaa
      781 aatcagatta aatctttgat ttttcattca acaacttgct ttttaaagct tctctgaaac
      841 caaaacatta aaacagcagc aattaaagca gttactgctc cgaaataaaa aggaacatgt
      901 gaatttactt tgtcgtacag tatgccggct ataaggctgg caggtagcaa ggttattccc
      961 aataatgcat tgtagagccc caaacccgtt ccttttttat tctcatcaac aatgtccgaa
     1021 acaaatgctt tctgtatgcc atcagtggca gcagaataga ctccatataa ggcaaagagt
     1081 gaagcaatta ccacgatatg actggaagct ccaaaaccaa aataaaccaa agcatatatt
     1141 atatacccga caatcaaaat tttttctttc cccactctgt cggataaact gccaactgga
     1201 atagacagca atacggaaac cacgttggtg agtaaataaa caagcggaat gaaggcaact
     1261 tttattccta tttcgttggc ttttaccagc agcaaggcat cggttgaatt tccaagcgta
     1321 aaaataaaaa ctatagccag aaagaaataa aattttttgg aaaaatcgcc aaaacgaaat
     1381 tttttcacca aactttcctt atttttctta gcctccgtta tgccgaaaat tatcaccaaa
     1441 attcccaata tggaaggaat gccggcgagc aaaaaaatca atctgtaatt gtctttgaat
     1501 gccgaaagca agataaatgc tatgagtgga cctaaaatgg caccactgtt gtccatagct
     1561 ttttgcagtc cgaaactttt tcctgtttcg ccattttcta ccgaaccggc tacaatgctg
     1621 tcgcgtggag ctgtgcgaat gcctttcccc actctttcca cgaaacgaag aatcagcacc
     1681 tgaatgggtg aaacaaccag cgagtacaaa ggtataataa cagccgaaac tccatagcca
     1741 atcagtaaaa atggtttgtt tttgcctatt ttgtcgctcc aaaaacccgt aattgttttg
     1801 atcagtgaag cagtactttc agcaactcct tcaataagcg ataaactggt ttttgatgcc
     1861 ccaatggaaa gcaaaaacat gggcatgacc gaatagacca tctttagtga agtgtccgtg
     1921 agaaaactgg ttaatccggt aaaaaaaata tttcgtggat atccaaaaat tcgtttattc
     1981 tttttcctgt tcatttttta atctttaata gaactaataa tttcaggaat ttgagatgca
     2041 aagatataaa ttttaactaa tcaaatcgag tagagattta aattcaatag caattttttg
     2101 gggattagtt tttcttc
//
LOCUS       DAOTAX010000152         2079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_40545_length_2079_cov_25.694664, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000152 DAOTAX010000000
VERSION     DAOTAX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40545_length_2079_cov_25.694664"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..832)
                     /locus_tag="PLM04_08795"
     CDS             complement(158..832)
                     /locus_tag="PLM04_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03204.1"
                     /translation="MNKDFFIKQLDDILNDYNRIKSKAQYDDLSGNTTIEEITTVLTK
                     AKAAIVRIVGDKSEYYKDTEATLKRTNIYEGTKLRNIIGTITALKSDLQNDYLKSFSD
                     IVQSEVFSDYLEMAEHLLNEGYKDPAAVLVSSTLEVHLRELCISNGIDIEVKNSKGNL
                     VAKKADLMNSDLAKANIYSSVYQKQIIAWLGIRNSAAHGKYSDYKTEEVSLMLQGIRQ
                     FILTTK"
     gene            complement(1028..1975)
                     /locus_tag="PLM04_08800"
     CDS             complement(1028..1975)
                     /locus_tag="PLM04_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03205.1"
                     /translation="MRKTTFTVLLTAFATIAFGQITTTKVTPKTDQIDTTPYDSTQNF
                     LGKDVYKYQGQELYLNAKSESLRQYGYDNFVLDYTQDKFTNKSNVYKCCDGYNSKYDE
                     LAGKYFKVLEVIKHPKAEQSEYLYGKKFYLKLQEKESNDIVYYEYDSEFEHSFPFIVV
                     GFFEKQKKFFVGREFVFNDSEFTDATDIQTGKTVTVKTGQKWKCIDLTIEDKYYNLSL
                     IFENSLGEKVADECDRVLNVAYTAKEADSYKKKFGETIWNTILASKVKIGMTKEMCKL
                     SWGEPKDINKTTTSGKTSEQWVYSDNYLYFDNGILTAIQ"
BASE COUNT          585 a          382 c          337 g          775 t
ORIGIN      
        1 aaacaaactc ttgcgaagca ggaaggctta tcccaatgag ctttccgctt caaaaaaaat
       61 agtattgaag ggacaagaaa tactgcataa cagaggtgct ttacatagga accgctatta
      121 gcggtagtgg ttattatatt tcttgtcgtg cctgtcatta tttagttgtc aagatgaatt
      181 gcctaatacc ttgaagcatc aacgatactt cttcggtctt gtagtcagaa tattttccgt
      241 gtgccgcaga attacgaata ccaagccaag caattatttg tttttgataa actgatgaat
      301 aaatatttgc cttggctaaa tcagagttca ttaagtccgc ttttttagca actaaatttc
      361 ctttactatt ttttacttca atgtcaatcc catttgaaat gcaaagttct cttaaatgaa
      421 cttctaatgt cgagctgact aaaactgccg ctgggtcttt gtatccttcg ttcaataaat
      481 gttctgccat ttccaaatag tcagaaaaaa cttctgattg aactatgtcg ctgaaagatt
      541 ttaaatagtc attctgcaag tcgcttttta aagctgtaat tgtcccaatt atatttctca
      601 gttttgttcc ttcgtaaatg ttcgttcgct tcagggtagc ttctgtgtct ttataatatt
      661 ccgatttgtc tcctacaatg cgaacaatgg ctgcttttgc tttagtcaaa actgttgtaa
      721 tttcttctat tgtcgtattt ccagataagt cgtcatattg tgcttttgac ttaattctat
      781 tatagtcatt cagtatgtcg tcaagttgtt ttatgaaaaa atctttgttc atttcttgtt
      841 gtcaatttat gtttatgtag gtcgtcctac cattaccgct aacggacaag tgtatgaaac
      901 gtggcaggtt gcgtagagcg tcattgtcag ccgacaatga cggttggagc gggctgatac
      961 gtttcgggca cgcacttacc ctgccatgtt ttatacatag tgttagcacc agtttttctg
     1021 tttgtcgcta ttgaattgca gtaagaatac cattatcaaa atacaaataa ttgtcggaat
     1081 acacccattg ttcacttgtc ttaccagaag ttgtggtttt gtttatgtct tttggttcac
     1141 cccaagaaag tttacacatt tcttttgtca taccgatttt tactttacta gccaaaatag
     1201 tattccaaat ggtctctccg aacttctttt tataactgtc agcttctttg gctgtataag
     1261 caacatttaa aactctatca cattcatctg ctactttttc tccgagtgaa ttttcaaaaa
     1321 tcaatgaaag attatagtat ttatcttcaa tggttaaatc aatgcacttc catttttgtc
     1381 ctgtttttac cgtaactgtt ttacccgttt gaatatcagt tgcatctgta aattcactat
     1441 cattgaaaac aaactctcta cccacaaaga attttttttg cttttcaaaa aagccaacga
     1501 ctataaatgg gaaagaatgt tcaaattcac tatcatactc gtaataaacg atatcattgc
     1561 tttctttttc ttgcaattta aggtaaaact ttttgccata taaatattca ctttgttcag
     1621 cctttgggtg ttttattacc tccaatacct taaaatattt tccagcaagt tcatcgtact
     1681 tagagttata gccatcacaa cacttatata cattggattt attagtaaat ttgtcttgtg
     1741 tgtaatctaa tacaaaattg tcataaccat attggcgaag actttcagac tttgcattca
     1801 agtagagttc ttgaccttga tatttataga catcttttcc caaaaaattt tgagtactgt
     1861 cataaggagt ggtgtcaatc tggtcggttt tcggagtgac ttttgttgtt gtaatttgtc
     1921 caaatgcaat tgtggcaaat gccgtcagca aaactgtgaa tgttgttttt ctcatattct
     1981 tgtctttttt ttaaaattgg tgccaactcg tttatatatc taccaaaagt aaggttacac
     2041 gcccaaaatg gacttgtata tccacttgat gatagttta
//
LOCUS       DAOTAX010000153         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_41120_length_2055_cov_32.919000, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000153 DAOTAX010000000
VERSION     DAOTAX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41120_length_2055_cov_32.919000"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLM04_08805"
     CDS             <1..363
                     /locus_tag="PLM04_08805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HON03206.1"
                     /translation="AEKIRVNQRERKKKISRRWAQHKSSTRKTKIVLAVMAAGIPVLA
                     VAPKNSEIALIVSESECGIVVEPGDVQALVSAIIFLKHNPERRKQMAINSRVAFENRY
                     TIENGLKKYLAIFNEVLR"
     gene            360..1634
                     /locus_tag="PLM04_08810"
     CDS             360..1634
                     /locus_tag="PLM04_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HON03207.1"
                     /translation="MKGKYKGILNQLYYFSLCLLAFTIPVSYRISLYVIGLALVLSVW
                     IASITGKLKMIYQNKVNLGFILFWFIHAVSLIYSSDLNYGLNDVFQKLSMVLLPIIFL
                     PFVPEAQVTERIKKWFLTGLFGASFFFLVRALILSTHFTPEGVTFKPNPSGVPWENYF
                     FYVRFVEPFHPTYFSMYLSLGIVFIASYVKKIKEIKSRALYYLAWIFLLVIVFFTSSR
                     IGILVSVLISLIATFWIIKRRGKLVVAVVLTFFTVVGGLILHKNERVGTSINQLDEYF
                     QGKEMENELARNSFVRFDIWRNFTDVFQDNSIFTGVGVGDVKRALVQVYEKKGIESAY
                     QNQLNAHNQFIQTLVACGVLGLLILLTIIGYAFWLAYKSRDWVLLLFMLIISINFMFE
                     SVLERVFGVIFFVFFHLFFTSNFMKEPKNRVD"
     gene            complement(1876..>2055)
                     /locus_tag="PLM04_08815"
     CDS             complement(1876..>2055)
                     /locus_tag="PLM04_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03208.1"
                     /translation="NINSEPITVVQDFTPLSKAENPYTKYAISLGLLFAVLGFVISIF
                     WQYKRDIYDLIFNRD"
BASE COUNT          595 a          307 c          413 g          740 t
ORIGIN      
        1 gcagagaaaa tccgcgtaaa tcagcgagaa agaaaaaaga agatttcacg cagatgggcg
       61 cagcacaaat catcaactag aaaaactaaa atagttctcg cagttatggc cgctggcata
      121 ccagtattag cagttgcccc taaaaattct gagattgctt tgattgttag cgagtcggag
      181 tgcggtattg tggttgaacc tggcgatgta caagctttag tttctgccat tatttttctg
      241 aaacacaatc ctgaaaggcg caagcagatg gctatcaatt caagggtggc atttgaaaat
      301 aggtacacca ttgaaaacgg tttaaaaaaa tatttggcaa tatttaatga ggtgttaaga
      361 tgaagggaaa atataaagga attctaaatc agctatacta tttttcgtta tgccttttag
      421 catttactat tcctgtaagt tataggattt cgttgtatgt tataggcctg gctttggttt
      481 tatcggtttg gatagcttcc attactggta agttaaaaat gatttaccag aataaggtta
      541 atttgggttt tattttgttt tggtttatcc atgctgtcag tctaatctat tcttcagatt
      601 tgaactatgg gttaaacgat gtttttcaga agctatcaat ggttttactg cctattatct
      661 ttttgccctt tgtgccagaa gcgcaagtga ctgaacgcat aaagaaatgg ttcttgacag
      721 gtttgtttgg tgcctcattt ttctttttgg ttcgtgctct cattctttca acccatttta
      781 ctcccgaggg ggttaccttt aaaccaaatc cgtccggagt tccctgggaa aactatttct
      841 tttatgtacg tttcgttgag ccgtttcacc ccacctattt ttcaatgtac ttatcgctgg
      901 gtattgtttt tattgccagt tatgtaaaga aaataaaaga aattaagtca agagcacttt
      961 attatttggc ttggatattc ttgcttgtta tagttttttt tacatcttca aggattggta
     1021 ttctcgtttc tgtattgatt tcattgattg caactttctg gattataaaa agaagaggga
     1081 agcttgtagt tgcagtggtt ttgacttttt ttactgttgt tggggggcta attttacata
     1141 aaaatgaaag agttgggaca tcaattaacc agttagatga atattttcaa gggaaggaaa
     1201 tggaaaatga gttggcaaga aatagctttg tgaggttcga tatctggcga aatttcactg
     1261 atgtttttca ggataatagt atttttactg gtgttggtgt aggcgatgtt aaacgggctc
     1321 ttgtgcaggt gtacgaaaag aaagggatag aatcagctta tcaaaaccaa ctgaatgccc
     1381 acaaccaatt cattcaaact ctagtggctt gtggtgtttt agggttactg attttgttaa
     1441 caattattgg ctatgcattt tggcttgcct ataagagtag ggattgggtt ttgttgctct
     1501 tcatgttaat tatttctatc aattttatgt ttgaatccgt acttgaacga gtttttggtg
     1561 tgattttctt tgtctttttt catttatttt ttacttcaaa ctttatgaaa gaacccaaga
     1621 atcgagttga ctaatcttca atttgtaagt tataagaaaa aagccaggtg tttcctggcc
     1681 ttttttgtat caatatatta gttgttagta gttttttgtt caacccaaat ccgtcaattg
     1741 aaaaaaacaa ttgacgaatc gattaacaaa ttctactcat tccgaaagtt tggaatgata
     1801 aattttgata agtaggggtt acctcgataa gtcaatagaa acaaagtatt tatatgtttt
     1861 ttgtttttct attgactaat ctcggttgaa tataagatcg taaatatccc ttttatactg
     1921 ccaaaaaatt gaaatgacaa atcctaatac agcaaagagt aaaccaagag atatagcgta
     1981 ctttgtgtat gggttttcgg cctttgaaag cggagtaaaa tcctgaacca ctgtaattgg
     2041 ctcactatta atgtt
//
LOCUS       DAOTAX010000154         2024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_41945_length_2024_cov_33.942610, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000154 DAOTAX010000000
VERSION     DAOTAX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41945_length_2024_cov_33.942610"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1928)
                     /locus_tag="PLM04_08820"
     CDS             complement(219..1928)
                     /locus_tag="PLM04_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HON03209.1"
                     /translation="MHGFNVLISKKAIKQQPHFCWKKPFDFQQNLALRTFSDEHIVVE
                     QFTGTKFISEKLWLDNEDFFIVTDGVITNLKALCLEHNAANYDALLRKIVKEKSFFKN
                     FRGHFVGFVYFKKEKTCIAFNNHTASKKLFYFADQNTIVIGTDLYTLSKTLETEKIKR
                     TLDKEAAVFLLTSGFMHENLTLLEEVKQLRAGEFLQIGNFNLKTDFYFHLQDIQENHD
                     AKSDIVEQLDFLFKKSIDNEFSLDKEHQLISLTTLSGGLDSRMVALTALQLGFSNQMF
                     LNFSAKGYADEIIARQISEQYQVPLETFSIEAESLKPLEEVVLVNDGLTIYTGASAVF
                     DAIKRHRITNTGMIHTGMLGDALLGSYLISDHKTKPQISDGVYSTKLLNKYAPVFQKY
                     IQNYSDIEMYKFYNRGFSGMNNGFLYFDLCGESDAAFLNPDFLSYALSIPAKYRYKEQ
                     IYIDWIKIKHPEFAGFTWESIGGKPTNNSILRCYYRYRRALVKRMPFQTMWKNNMNPE
                     QLWYDKNLSLRQTLDQYFLENIHLLSSCSDLFDDCQFLYKKGNITEKAQVLTLLAACK
                     LLL"
BASE COUNT          644 a          380 c          348 g          652 t
ORIGIN      
        1 ccaattttca attcgatttt tctacctgat gatgctaatg gacaatttgg gattattttc
       61 ttaacttact gtaatacaaa tatatgaatt ttcaaaaaca acatttttaa aaactttcgt
      121 acggcagtga aattttgtat ccttttttat ctcaatgtct taaaaaaact aattctcttt
      181 gagtaacttt tcggagaaga cttatttatt tatttattct acaacaacaa tttacaggca
      241 gccagtaaag taagcacttg cgctttctcc gtaatgtttc ctttcttata aaggaattga
      301 caatcgtcga ataaatcgct gcaggatgag agcaaatgaa tattttccaa aaaatattga
      361 tccagcgttt gccgaagcga caagttttta tcgtaccaaa gctgctcggg attcatgttg
      421 tttttccaca tggtttgaaa aggcatccgt ttcactaaag cacgacgata acgataataa
      481 caccgcaata tactgttgtt ggttggtttc ccacctatgg attcccatgt aaaaccggca
      541 aattcgggat gtttgatttt tatccagtcg atataaattt gttctttata tcggtatttt
      601 gcgggtattg aaagagcata agacaaaaaa tcgggattta aaaaagcagc atccgattct
      661 ccacacaaat cgaaatacaa aaaaccgtta ttcatgccgg aaaaacctct gttgtaaaat
      721 ttatacattt caatgtcaga ataattttga atgtatttct gaaaaacggg cgcatattta
      781 ttcaataatt tggtagaata gacaccatca gaaatttgcg gtttggtttt atgatccgaa
      841 atcagataac ttcccaacag agcatctccc agcataccgg tatgaatcat gcccgtattg
      901 gtaattctgt gccgttttat ggcatcaaac actgcacttg ctccggtata aatggttagt
      961 ccatcgttca ccagcacgac ctcttcaaga ggtttcagac tttcggcttc aatagaaaaa
     1021 gtttccaacg gaacttgata ttgttcagaa atttgccgcg caattatttc atcagcatag
     1081 ccctttgcag aaaaatttaa aaacatctgg tttgaaaagc ccaactgcaa agccgtgagt
     1141 gccaccattc gcgaatcgag gcctccgctc agagtagtca gagaaatcaa ttgatgttcc
     1201 ttgtcaagag agaattcatt atcaatcgac ttcttaaaaa gaaaatcgag ttgttcgaca
     1261 atatcggatt ttgcatcgtg attttcttga atatcctgaa gatgaaaata aaaatctgtc
     1321 tttaagttga aattgcctat ttgcaaaaat tctccggccc gcaattgctt cacttcttcc
     1381 aacaaggtta agttttcgtg cataaatcca cttgtgagta aaaaaacggc ggcttccttg
     1441 tctaaagttc tttttatctt ttcggtttcg agagttttgc tcagtgtgta caaatcggta
     1501 cctatcacaa tggtattttg atcggcaaaa taaaaaagtt ttttcgatgc cgtgtgatta
     1561 ttgaaagcga tacatgtttt ctcttttttg aaatatacaa atccgacaaa atgtccccga
     1621 aaatttttaa aaaaagactt ttcttttact attttcctga gcaacgcatc ataatttgct
     1681 gcattatgtt ccaagcaaag ggctttcagg ttggtaatga cgccatcggt aacaataaaa
     1741 aaatcttcat tgtccagcca caacttttcc gaaataaact tcgtcccggt aaattgttcc
     1801 accacaatat gttcgtcaga aaaagtgcga agcgcaaggt tttgctgaaa gtcaaaaggc
     1861 ttcttccaac agaaatgggg ttgctgcttt atggcttttt ttgaaataag aacattaaat
     1921 ccgtgcatag aaccgtttct gaaataattg ttacgaactg cgttggcaat aaaatgaatt
     1981 tacaatgaaa aaacttaatc aggcaacaaa acttgttgat tgaa
//
LOCUS       DAOTAX010000155         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_42008_length_2022_cov_37.162176, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000155 DAOTAX010000000
VERSION     DAOTAX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42008_length_2022_cov_37.162176"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..499
                     /locus_tag="PLM04_08825"
                     /pseudo
     CDS             383..499
                     /locus_tag="PLM04_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            929..1135
                     /locus_tag="PLM04_08830"
     CDS             929..1135
                     /locus_tag="PLM04_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HON03210.1"
                     /translation="MATARKPKTGIQKPLNLQRHGHSYASHLKDGGTDLGYIQSLLGH
                     LSSNTTVIYTHVYTKKLQKIRSPD"
     gene            1509..1943
                     /locus_tag="PLM04_08835"
     CDS             1509..1943
                     /locus_tag="PLM04_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HON03211.1"
                     /translation="MTSTTTDIVKEIAEQLDCGFRAFIHKTTGQLLFIPDNYNYPDID
                     LSSWDKELEQLENNFTDYHEIEKWTSSEAFEIMSEFAVHLTDKDLQSRLFDTLRKNKP
                     FKEFKFVIDNSGDFRQQWFDFKNKWQEDFVARQLNRLKTTDE"
BASE COUNT          693 a          374 c          387 g          568 t
ORIGIN      
        1 tgatgaaaag ttttggaaaa atttttaggg tgacgcattg tcgaagtcag gagatttgtt
       61 ttaagctcta gaggttgtat tttcttctaa ataatttttt catcaactat tttcaataca
      121 aaatgtaaag caatattgat tacagttaat tatgtatttt gagcaacgaa tgggcaacaa
      181 aaaatcaact gaaatcgtaa tgccgtaatg taaagatagg aagaattttg ctgaattttg
      241 ggcaataaaa aacccctaat tttcaccaag ggctctgttc aaattaaagt ttctgctatt
      301 taaccttgct ggctaattca ttccctgtga gatggtctaa aaaaactgga caggaattta
      361 gtaattttct aatttctgtc atataaaaaa gtcaagaaga acatttacgg ctgaatttag
      421 agcaaagatt gccatagagg cgataaaaga gaacaagacc ctatctgagc ttgctcagat
      481 ttaccaggtg catcctaatt taatgattca tgaatgagga agttttaggg tcaatgttgt
      541 gcttattctc tcgaagctta ttgtggcttg gaaagatgga aaaagtgaag ttgtgtcaga
      601 ggcacaggta ataaatgaca ggtgacggag agattgggaa taaattgctg gaatcaaatt
      661 ccataatatt ttacgcctga ctttgggtga ggtgaggtat agccagggct gatgagggtg
      721 tgccagttgc ggccgaaacc actcattgtc ctttcaccat cgcatggccg cccatcacaa
      781 aaaaattttt caaagacctt gcaaaattca aaattttcac tatttttaca tcgttcgtca
      841 gccaagctaa ttgtgataga aagtctctgg gaaaattcaa gggctgacgt aaagaaagcg
      901 tgttcgatat aggttcaaaa cttcggcaat ggctacagct cggaaaccaa aaaccggtat
      961 tcaaaagccg ttaaacttgc aacggcatgg gcacagttat gcttctcatt tgaaggatgg
     1021 cggaaccgat ctaggttaca tacaatccct gcttggccac ctttcgagca atactaccgt
     1081 gatctacacc catgtttata ctaaaaaact tcaaaaaatc agatccccag attgatgata
     1141 tgtaacaaaa tcataccttt aaaaggaaat aagaaacgat taaaaccgta cttatacagc
     1201 caaattggga aatattgtat atttgtttcg tacatataaa cgagttaggt gaaaccataa
     1261 tgagacgaca gttcaactat ctaccaagac ttttgaagac tctgaatgac tgtccccctg
     1321 ttcgactttt ttcggctgac tttgacaact tttcaacttt gtgcttccta cccacttact
     1381 gtttcggagg acagtaattc aggtctttaa aaacgtaata aacggtcgga caggcaactc
     1441 actatgataa ctactaactc ttcggaacag aaaagaaatt tttaaaattt gacaggtaac
     1501 aaaaatatat gaccagcacg acaacagaca tagtaaaaga aattgcagag caacttgact
     1561 gcggttttcg tgcattcatt cacaagacaa caggacaact ccttttcata cctgacaact
     1621 acaattatcc cgacattgac cttagctcgt gggacaaaga acttgaacaa ttagaaaaca
     1681 acttcaccga ctatcacgaa attgaaaagt ggacttcaag cgaagcgttt gaaataatga
     1741 gtgagtttgc cgtccatctt accgacaaag atttgcaaag ccgactattt gacacgttga
     1801 gaaagaataa accgtttaaa gaatttaaat tcgtaattga caactcaggc gacttcagac
     1861 agcaatggtt tgactttaaa aacaagtggc aggaggactt tgtagcaaga caattaaacc
     1921 gactaaaaac aacagacgaa tgaaaaaaat ggcagcacct aacagctgtt cctatgtaaa
     1981 gcacctctgt taagcagtat ttcttgtccc ttcaatacta tt
//
LOCUS       DAOTAX010000156         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_42364_length_2009_cov_26.926305, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000156 DAOTAX010000000
VERSION     DAOTAX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42364_length_2009_cov_26.926305"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PLM04_08840"
     CDS             <1..1193
                     /locus_tag="PLM04_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HON03212.1"
                     /translation="VMHKAKTTTNNANITTMKLKAKIAILIATILPLLSFAQKTIELP
                     YVETNSHVQSLLIKSIEISNDYTVVNIRYVNSLGAGWIRTYKPGDKKAYYISDQYGKR
                     IAELVSAKNIPFAPKKYIMRSYNEIVDFTLVFSSIDKNIKKIDIIEGNADDQGNFNFY
                     GVYLDGTVAKGEKPKGMEINGKKEQVGSGTGFILNTDGFIATNYHVIDEAKEIEVVFP
                     SEEGIKTYKVSVFLADKTNDIAILKINDSTFKKLTYLPYELSDDYEVGEEVFTIGYPQ
                     PDIMGTESKLTTGIINSLSGIDNDNTCMQISVPIQPGNSGGALFNSKGNIVGITTSSL
                     NSIFMAKYKGNIPQNVNYAVKTEYLKVLTKSVYLDGKNIVKDMSLKDKAKIIKNYICL
                     IKTY"
     gene            1199..1822
                     /locus_tag="PLM04_08845"
     CDS             1199..1822
                     /locus_tag="PLM04_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03213.1"
                     /translation="MECTTVNMIFQIIGAIGSLATFGAFVFLFRRDKDKQTQIDKLTG
                     IATILEAQNETMKLQNDLIAQQVEIFRNTSILKGQDDKALGQLKEIEERKLKLSVKPN
                     LWLNGAGYKGFDGELTIDLNNKGEDAKLIEFKLTSDDIILHSLSLPYDLDKGQRRYIF
                     GRQKGNKHIKDCDYEIDVIYQDKLENKYLSKIKGTGANAKIIETREI"
BASE COUNT          798 a          308 c          359 g          544 t
ORIGIN      
        1 ccgttatgca caaggctaag acaacaacaa acaacgctaa tattacgaca atgaaattaa
       61 aagcaaaaat cgcaatccta attgcaacaa ttttaccctt attgtccttt gctcaaaaga
      121 caattgaatt accatatgtt gagacaaatt ctcacgttca atctttgctt ataaaaagca
      181 tagaaatctc taacgactat acagtagtaa acataaggta tgttaactca ttgggagctg
      241 gttggatacg gacttataaa ccgggcgaca aaaaagcgta ttacatctct gaccaatatg
      301 gaaaaagaat tgcagaatta gtgagtgcga aaaacatccc atttgcaccg aaaaaataca
      361 taatgcgttc atataacgaa atagttgatt tcactctagt ttttagttca attgacaaaa
      421 acataaagaa aatagatata attgaaggta atgcggacga ccaaggtaat tttaattttt
      481 atggagtata tcttgatggg actgtagcga aaggtgaaaa gccaaaagga atggaaatta
      541 atggaaagaa agaacaggtt ggttctggaa caggttttat cctgaataca gatggtttta
      601 ttgctacaaa ttatcatgta atagatgaag caaaagaaat tgaagttgtt tttccttcag
      661 aagaaggaat aaaaacatat aaagtaagtg tgtttttggc agacaaaacg aatgatattg
      721 caattctaaa aatcaacgat agtacgttca aaaaattaac ttatctgcca tatgaattat
      781 cagatgatta tgaagtggga gaagaagtat ttacgattgg ctatccacaa ccagacatta
      841 tgggtacaga atcaaaactt acaacaggca ttattaattc attatcaggg atagataatg
      901 ataatacttg tatgcagatt tctgttccaa tccaaccagg caatagcggt ggagctcttt
      961 ttaatagtaa agggaatatt gttggaatta caacaagttc attaaactca atattcatgg
     1021 caaaatataa aggcaacatt ccacaaaatg taaattatgc tgtaaaaacc gagtatctga
     1081 aagtcttaac caaatccgtt tatttggacg ggaaaaatat agttaaggac atgtccttga
     1141 aggacaaggc taaaataatt aagaactata tctgccttat caaaacatat tagtaacaat
     1201 ggaatgcacc acagttaata tgattttcca aattattggg gcaattggct cacttgctac
     1261 ttttggagca tttgtttttc ttttccgtag agacaaagac aaacaaaccc aaatagacaa
     1321 acttactggt atagctacaa ttttagaagc tcaaaacgag acaatgaaat tgcaaaatga
     1381 tttgattgca cagcaagttg aaatatttag aaatacgtca atcctgaaag gacaggatga
     1441 caaagcatta ggtcaattaa aagaaatcga agagagaaaa ttaaaattga gtgtaaaacc
     1501 caatctttgg ctaaatggtg caggctacaa aggatttgac ggggaattga caattgactt
     1561 aaacaataaa ggagaagacg ctaaacttat agagttcaaa ttaacttcgg atgacattat
     1621 tctacatagc ttatcattac cctacgactt ggacaaggga caaagaagat atattttcgg
     1681 gcgacaaaaa ggaaacaaac acattaagga ttgtgattac gaaatagatg taatatacca
     1741 agacaaactt gaaaataaat atttgagtaa aataaaagga actggagcaa atgcaaaaat
     1801 cattgaaaca agggaaatat aaaacagccc agtgcataac aaggtattac caaaagcggg
     1861 gctgaacagc ttcgattgga catttgtgca aagtttaaca ttcattcttc aattgaactt
     1921 ttgtactaaa aattctcacc ttcggcaata ccagaaccgt tacctgcaac tctaagcaaa
     1981 acattttaca ggttaaaagg aacaataat
//
LOCUS       DAOTAX010000157         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_44118_length_1946_cov_34.835537, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000157 DAOTAX010000000
VERSION     DAOTAX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44118_length_1946_cov_34.835537"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..704
                     /locus_tag="PLM04_08850"
     CDS             129..704
                     /locus_tag="PLM04_08850"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HON03214.1"
                     /translation="MEKLLQEEIRKAVEVLRQGGTILYPTDTVWGIGCDATSAEAVKK
                     VYEIKRRADSKALLVLVDSASRILSYVDEVPDMAWQLIEITDKPLTIIYPEAKNLAAN
                     LIAADRSIGIRVTRELFSKNLCEQFRKPIVSTSANISGNPTPSNFSQIDEAIKSAVDY
                     VVNWRQDDISEPKPSSIVKLSKGNVIQIIRE"
     gene            complement(1217..1816)
                     /locus_tag="PLM04_08855"
     CDS             complement(1217..1816)
                     /locus_tag="PLM04_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HON03215.1"
                     /translation="MSSEIKLTITGLVYNQNIVGTYGLVLSEESGNRRFSVMIGEPEA
                     QSIALKLNNKKLPRPLTHDLIKTILDVFEAKLVKVLISDMINDVFYSELHILKDEKIL
                     VVDARTSDAVALAVRCNSPIYIKSDILDIVGVVVEENEIEETTDNEENIEELSDQELD
                     NLSLNELEELLSMAVNEEKYELAVHIRDAIQRKKTKEKF"
BASE COUNT          605 a          338 c          332 g          671 t
ORIGIN      
        1 aggaaatctc agtttcggta gaagtaaaac ttgggtggaa taatttatcc ttttttgaag
       61 taaggcgtat ttttgttatt tttgcttgga gttttcattg aaggtttgga ttaaactatc
      121 attacaaaat ggaaaaatta ttacaggaag aaatcagaaa agctgttgaa gtgttgaggc
      181 aaggaggtac tatactttat cccaccgata cggtatgggg aattggttgc gatgccacca
      241 gcgcagaggc cgttaagaaa gtttacgaaa taaagcggcg agccgatagc aaagccttat
      301 tagttttggt tgattccgcc tcgagaatac tttcttatgt ggacgaggtg cccgatatgg
      361 cttggcagtt gatcgaaatt accgataaac ctctcaccat tatttatccc gaagccaaaa
      421 atctggctgc caatttgatt gctgctgatc gttcaatagg catcagggta acaagggaat
      481 tgttttcaaa aaacttgtgt gaacaattca gaaaacccat tgtttccacg tctgccaata
      541 tcagcgggaa ccccacgccg tcaaattttt cgcaaataga tgaagccata aaatcggctg
      601 tcgattatgt ggtaaattgg cgacaggatg acatttccga acccaaacct tcttccattg
      661 tcaagttgag taaaggaaac gtaatccaaa ttatcaggga ataaaatcgc aaaaagtttt
      721 ttgcatccgt actttttctg ttgtttatac taaatacatt aaggttacat tttttatttt
      781 gatgaaagaa ctttgatact atgacttttt cctgtaagtt tttatagcca acgaatatcg
      841 aaaaaattct tcatatgata aagaatagtt gaataaccag gtctaatttg ttgtaaaaag
      901 ttcacaagtt gtacattaat ttcatcagaa tataatttat tttttataag gtctgatgga
      961 acccacatgt ctgaatatta tattcattcg tgtttgtgta tgcaatacat gtggctttca
     1021 taacatcatt cttgattaat tttacaatat catttgctat gcatactaac agaaaaaaat
     1081 tataattaaa ctttccaata gtatcgaccc attgcttttg gtttatagta ttttaatttt
     1141 caattcaata attcgataaa gtgaagcaat tccgtagtca tcaacttatg attgaaacaa
     1201 atttattttg agaaaatcaa aatttttctt ttgttttttt gcgttggata gcatctcgga
     1261 tgtgtacagc cagttcatat ttttcttcgt ttacagccat agaaagtaat tcttccaatt
     1321 cgttgagcga aaggttgtcc aattcctgat cggataactc ttcgatattt tcttcgttgt
     1381 cggttgtttc ttctatttcg ttttcttcca caacaacacc tacaatatcc agaatatccg
     1441 atttgatgta tattggactg ttacatctaa ccgctaatgc cacagcatct gaagttcgtg
     1501 catcaaccac caatattttc tcatctttga gaatatgcaa ttcagagtaa aaaacatcat
     1561 ttatcatgtc gctaatcaac actttcacca gttttgcttc aaaaacgtct aaaatagttt
     1621 ttatcagatc gtgagtgagt ggacgtggaa gttttttatt gttgagcttc aatgcgatag
     1681 attgcgcttc gggttctcca atcatcacgg aaaatcgtcg attgcccgat tcttcactaa
     1741 gaaccaagcc gtaagtaccc acaatgtttt gattataaac caaccctgtt atcgttaatt
     1801 ttatttctga actcatacag actatttagt tgcaattagt tgacaaaatg acttttatca
     1861 ttaaactcga attgttattc tttctttatg acaaaggtac acaatttttt taaaaacgat
     1921 aaccaaaaat gaatacatag tttttc
//
LOCUS       DAOTAX010000158         1923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_44810_length_1923_cov_27.808887, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000158 DAOTAX010000000
VERSION     DAOTAX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44810_length_1923_cov_27.808887"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1923)
                     /locus_tag="PLM04_08860"
     CDS             complement(<1..>1923)
                     /locus_tag="PLM04_08860"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HON03216.1"
                     /translation="EENFQQIEYSFGYNDQTTFKDSYHLPTQTLDVFGLGSLQRDLPA
                     DFPANLNSLSIQQRDAAALKLNNHWVAQPYTALPNQKLTFSIGRKWNLKKGNKIGNLT
                     SLTYSNSSSSRFNMENNMYERFDPIAETPVYLYNYAADIYSTDFKIGLMHNWAYIKTN
                     GTKIEFKNLLNQIGVDKTSNTFGWNNYRQGNFKYFSNQYSARTTYSGQLSGNHKLKNN
                     EDNKLDWNVGYAYANRIEPDRQNWSMKETQGGIYEYVLPDVPSINELGRLYMNNHENV
                     MVSGINHERKFQFLNTLPVLKSGLYGEFKSRNFSERSITYRKAYGPLGNDEINALDFE
                     SLFTETYLGQGKVISVDEQTNVANTYKAQNWLGAGYLSMTLPAGKFNLSGGIRAEYNH
                     LLLDGYYNPTSPVHVDNPTFNIFPSINTSYNLSDKSLIRLAYAHTINRPEFREVSPLS
                     YYDFTEKNSVRGNPNLQDASIQNIDLRFENYPSPTETFSLAAFYKHFTHPIEMVNNGV
                     GNDFSFGNAKAAVNYGLELEAKKSLEKLIGLKNFTLQVNASYIFSKVEFADKANEQNR
                     PLQGQSPYVVNAALFYQNDKAGLSTSVMYNVMGKRILAAAQLNQGEVVIPDIYEMPRH
                     VVDFTLNKKLGNQMEL"
BASE COUNT          521 a          371 c          391 g          640 t
ORIGIN      
        1 ttaactccat ctgattaccc agctttttgt ttaatgtgaa atcgaccaca tgacgaggca
       61 tttcataaat atcggggatt actacttctc cctgattcaa ctgtgctgca gccaaaattc
      121 tttttcccat cacattgtac atcacagaag ttgacaaacc ggctttatca ttctggtaaa
      181 ataacgctgc gttaactaca tacggcgatt gtccctgcaa tggtcgattt tgttcgtttg
      241 ccttatcggc aaattccact ttactgaaaa tgtaggaggc attcacctga agagtaaaat
      301 tctttaaccc gataagtttt tccagcgatt tttttgcttc cagttccagt ccgtaattaa
      361 cagccgcttt ggcgtttccg aaagagaaat cgtttcctac gccattgttt accatttcga
      421 tcgggtgcgt gaaatgcttg tagaaagctg ccagcgaaaa agtttctgtc ggagatggat
      481 agttttcaaa tcgcaaatcg atattttgaa tggaagcatc ctgcaaattg ggattacctc
      541 ttactgaatt tttttcggta aaatcatagt aactcaaagg cgaaacttca cgaaattcgg
      601 ggcgattgat ggtatgagca taagctaacc tgataagaga tttgtcggat aaattgtaag
      661 aagtatttat ggaaggaaaa atattgaaag tgggattatc aacgtgcact ggtgaagttg
      721 gattgtagta gccatccagc agcaaatgat tgtattctgc tctgattcct ccactcaaat
      781 tgaattttcc tgccggcaat gtcatggaca aatatccagc accaagccaa ttctgtgcct
      841 tgtaggtgtt agcaacgttg gtttgttcat ctacgctaat aacttttcct tgtccgaggt
      901 aagtttcagt gaaaagactt tcaaagtcga gtgcattaat ctcatcatta cccaatggtc
      961 cgtatgcctt gcgataggta atgcttcgtt ccgaaaaatt gcgcgatttg aattccccat
     1021 agagtcctga tttcaaaaca ggcaacgtat tgagaaattg gaattttctt tcgtgattta
     1081 tcccagacac cataacattt tcgtgattgt tcatgtaaag ccttcccaat tcgttaatgc
     1141 tgggaacatc aggcaacaca tattcgtaaa ttcctccttg agtttctttc atgctccaat
     1201 tttgacgatc cggttcgatt cggttggcgt aagcataacc cacattccaa tccagtttgt
     1261 tgtcttcatt gtttttcaac ttgtggttac cgctcaactg tcctgaatag gtggtacgtg
     1321 cagaatactg attgctgaag tatttgaaat ttccttgacg gtaattgttc cagccaaaag
     1381 tattggaagt tttatctact ccaatttggt tcaacaggtt tttaaattct atttttgttc
     1441 catttgtttt aatgtaagcc caattgtgca tcagtccgat tttaaaatcg gtactgtaga
     1501 tgtcggctgc atagttgtaa agataaacag gtgtttctgc tatggggtca aagcgttcgt
     1561 acatgttatt ctccatattg aaacgtgaag aagatgagtt gctgtaggtg agtgaagtaa
     1621 ggttacctat tttgttccct tttttcaaat tccattttct tccaatagaa aaagtcaatt
     1681 tttgatttgg caatgccgtg taaggttgtg caacccagtg gttgttcagt tttaaagctg
     1741 cagcatcccg ctgttgaata ctgagggagt tcagatttgc ggggaagtcg gcaggaagat
     1801 ctctttgcag gctacctaaa ccaaaaacgt ccaatgtttg agtcggtaaa tgatagctgt
     1861 ctttaaaagt agtctggtca ttgtatccaa aactgtattc gatttgttga aaattttctt
     1921 ccg
//
LOCUS       DAOTAX010000159         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_45301_length_1907_cov_28.807235, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000159 DAOTAX010000000
VERSION     DAOTAX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45301_length_1907_cov_28.807235"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLM04_08865"
     CDS             <1..207
                     /locus_tag="PLM04_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03217.1"
                     /translation="RHVVDFTLNKKLGNQMELKFGIKDLLAQNYKTQQTYEYEKDGTT
                     KKATLTNQSYNLGRTFSLALNWKF"
     gene            441..1829
                     /locus_tag="PLM04_08870"
     CDS             441..1829
                     /locus_tag="PLM04_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03218.1"
                     /translation="MKRNFLNYFVISSVMVSGLIFFTACDDKSDPDPEPTPTSNEVIL
                     NSDITGTRNLVNDSVYILQGQVNVSGTINIEKGTIIKGDKLTKGCLVVLRGGVINAIG
                     TPTEPIVFTSRMEPGLRNAGDWGGIIIVGNAPVNQSNPSVEGLTREVTYGGGTNPADN
                     SGSLRYVRIEFAGIALQPDKEINGLTLCAVGSGTTIDHIQVSYSGDDSFEWFGGTVNA
                     KYLIAYCGLDDEFDTDFGYSGHVQFALGVRNPRVADVSTSNGFESDNDGSGTGATPQT
                     SAVFSNVTLIGPYKTTADKNVNSMFGAGMHIRRNSSISVFNSVFAGWNTGLLLDGTST
                     YANATAGNLVIQNCVLSGTKGTAVNGAGGVTADQALTFFNSGNNQIIADNATTGIASS
                     FYDATKTGAEAAPSSFLPAAGSPLLSGASFTHAKLADNFFDKTPTFKGAFGTTDWTKE
                     QWTNFDPQNTVY"
BASE COUNT          576 a          366 c          386 g          579 t
ORIGIN      
        1 cgtcatgtgg tcgatttcac attaaacaaa aagctgggta atcagatgga gttaaaattt
       61 ggtatcaagg acttgcttgc tcaaaattac aaaactcagc aaacctatga gtatgaaaaa
      121 gatggtacaa caaaaaaagc aacgctgacc aatcaaagtt ataatcttgg cagaaccttt
      181 tcgctggcac tcaattggaa attttaattg tgtctgtcaa attatatgga atcggcaaac
      241 tgtttgagaa ttttgagttt ttcgaaacgt atgacatcat gattcctgta atacattctt
      301 catatctttt acatataacc atcattgttt cggaataatc ttgtaacagt caaccattac
      361 ttttgtccta taaaaaaaat tttagaataa gccgagcatc tacggtaagt ttcaatatgt
      421 aaaatttaat caacacgttt atgaaaagga attttttaaa ttatttcgtt atttcttctg
      481 tcatggtttc cggtttaatt ttctttacgg cttgtgatga caagtcagat cccgatccgg
      541 aaccaacccc tacttcaaat gaagtaatct taaattcgga tatcaccgga acacgcaatt
      601 tggtaaacga ttccgtttat attcttcaag gacaggtcaa tgtaagtgga accatcaata
      661 ttgaaaaagg aaccattatt aaaggtgaca aattgaccaa aggttgtttg gttgttttgc
      721 gtggtggtgt aatcaatgct attggaactc ccacagaacc tatagttttc acttcacgca
      781 tggaacccgg attacgcaac gcaggtgatt ggggtggaat cattatagta ggaaatgcac
      841 cggttaacca atccaatcca agtgtagaag gtttgactcg ggaagttact tacggtggtg
      901 gtacgaatcc agccgataat tccggttcac ttcgttatgt tcgcattgaa tttgccggaa
      961 tagccctgca gccagataag gaaattaacg gactgacttt gtgtgcggta ggaagtggaa
     1021 caaccattga ccatatccaa gtttcctatt caggagatga ttctttcgaa tggtttggtg
     1081 gtacggtaaa tgcaaaatat ttgatagcct attgcggatt ggatgatgaa tttgacaccg
     1141 atttcggtta ttcggggcat gttcaatttg ctttaggagt tcgtaatccc cgcgtcgccg
     1201 atgtttcaac ttccaacggt ttcgaatcgg ataacgatgg ctctggtact ggcgcaacac
     1261 ctcaaacttc tgcagtattt tcaaatgtta ctctgatagg accttacaaa acaacagccg
     1321 ataaaaatgt aaacagcatg tttggagcag gaatgcatat tcgtcgcaac agctcaattt
     1381 cagtctttaa ttcggttttt gcaggatgga atacaggcct gttactggat gggacatcta
     1441 cttatgccaa tgcaactgca ggcaatttgg taattcaaaa ttgcgtattg tccggtacaa
     1501 aaggaacagc tgtgaacgga gcaggaggag taactgctga tcaggctctg acattcttca
     1561 acagcgggaa taaccaaatc attgcagata atgctacaac aggaattgcc agttctttct
     1621 acgatgcaac caaaacaggc gctgaagctg ccccttcctc tttcctccct gctgcaggtt
     1681 cgccattgtt gagtggagca tcatttaccc atgcaaaact ggctgacaat ttcttcgaca
     1741 aaactccaac ttttaaaggt gcctttggta caacagattg gacaaaagaa caatggacca
     1801 attttgatcc acagaataca gtttattaat cattttttcg tctgtttttt taagggcatt
     1861 ctaaaatcgg gatgcccttt ttaattctat gtaagtgttt taaacac
//
LOCUS       DAOTAX010000160         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_45995_length_1884_cov_20.704210, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000160 DAOTAX010000000
VERSION     DAOTAX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45995_length_1884_cov_20.704210"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1794)
                     /locus_tag="PLM04_08875"
     CDS             complement(139..1794)
                     /locus_tag="PLM04_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HON03219.1"
                     /translation="MRKKYFVFILMSIIFCSCKTEEPQVNTINAYIQNVSINGISVSS
                     GGTVYNIPVDSIVFTIDFSEAIDKSKINPQHLYLNNGIDTSFMILNDTSSNKLIFRIK
                     NRLEHLTRYTLYIAEGENLGVNLIDSYQFSFITQLDSTPKFSEISDDSLLTLVQKQTF
                     RYFWDYGHPVSGLARERTGSGETVTIGGSGFGVMATLVGIERGFITRQQGFERLSTIV
                     NFLNTKADRFHGVFSHWLNGTTGKVQPFSEKDNGADLVETAFLMEGLLTVQQYFKNGN
                     REEKNMCDTIQKIWENVEWNWFQQNGQQKLYWHWSPNYGWAMNMPITGWNEALIIYIL
                     AAASPTHSIEKTVYDNGWARNGAIRNGKTFYDILLPLGEDRGGPLFFTHYSFLGLDPR
                     ILSDAYANYWTQNTAHAQINYRYCVANPKKNFGYSRDCWGLTASDAPNGYTASSPNND
                     NGTIAPTAALSSFPYTPEESMQALHFFYYILGDKIWGEYGFMDAFNLNKQWFSNSYLA
                     IDEGPIIGMIENYRTGLLWNLFMQNEDVQRGLEKLEFDVNRIF"
BASE COUNT          578 a          371 c          337 g          598 t
ORIGIN      
        1 agacataagg agggaatgtt tttcctcctt tgtcttgcaa aataattttt tgtttacctt
       61 ttgcctcccc cctgaaaaag ttgcattaat tacttaaatt tacgtttcat aatttcaatt
      121 accctgttga aaaattactc aaaaaatcct gttaacgtca aactccaatt tttccaatcc
      181 tcgttgtaca tcttcatttt gcatgaataa attccaaagt aatccggttc ggtaattttc
      241 aatcattccg ataatgggac cttcgtcaat ggctaaatag gaattggaaa accattgttt
      301 gttcaaattg aacgcatcca taaaaccata ttcgccccag attttatctc ccaatatata
      361 ataaaagaaa tgtaaggctt gcatactctc ttcaggtgtg tagggaaagg atgacaaagc
      421 tgccgtaggt gcaatagttc cattgtcgtt attcggtgag gaagcagtat aaccgtttgg
      481 agcgtcgctg gcagtcagac cccagcaatc tcggctgtaa ccaaaatttt ttttcggatt
      541 ggctacgcaa tagcgataat tgatttgagc atgagctgta ttctgcgtcc aataattggc
      601 ataagcatcc gataaaattc ttggatcaag tcccaaaaat gaatagtgag taaaaaatag
      661 cggaccacct cgatcttcac ccagtggaag cagaatgtcg taaaaagttt ttccgtttcg
      721 tatagctccg tttcgtgccc agccattgtc ataaactgtt ttttcgattg aatgagttgg
      781 agacgcagct gccagaatat agataatcag agcttcgttc caacctgtga tgggcatgtt
      841 cattgcccag ccatagtttg gcgaccaatg ccagtaaagt ttttgctgtc cgttttgttg
      901 gaaccagttc cattctacat tttcccatat tttttgaatg gtatcacaca tatttttttc
      961 ttcgcgattt ccatttttaa aatactgttg aaccgtcaat aacccttcca tcaaaaaagc
     1021 cgtttctact aaatcggcac cgttatcttt ctcgctgaat ggttgaactt ttccagttgt
     1081 tccattcaac caatgagaaa aaacaccgtg aaaacgatcg gcttttgtgt tcaggaaatt
     1141 tacaatggtg gacagtcttt caaaaccctg ctgacgggta ataaaacctc tttcaatgcc
     1201 cacgagcgtt gccatcactc caaaaccaga accgccaata gttaccgttt cacccgaacc
     1261 agtgcgttct cttgcaagcc ctgaaacagg atggccgtaa tcccaaaagt atcggaacgt
     1321 ctgtttttga accaatgtta acaagctatc atctgatatc tcagaaaatt taggtgtaga
     1381 atcaagttgg gtaatgaatg agaattgata gctgtctatc aaattgacgc ccaaattttc
     1441 tccttccgca atataaagcg tatagcgggt taaatgttca agtcgatttt ttatcctgaa
     1501 aatcaattta ttagaagatg tatcgtttaa tatcataaaa gaagtatcga taccattatt
     1561 caaatacaaa tgttgtggat tgattttaga tttatcgatg gcttcggaaa aatcaattgt
     1621 gaaaacgatc gaatctactg gtatattgta aactgttcca ccagaggata cagaaatgcc
     1681 gttaatactc acattttgaa tatatgcgtt gattgtatta acttgaggct cttcggtttt
     1741 gcagctgcaa aaaataattg acattaagat aaaaacaaaa tattttttcc gcatggaata
     1801 cattgctaaa aaattcaggg aagatgaatt tcatcttccc tgaatacagt aagagataaa
     1861 ttaattaatt tgtgtaactt cggc
//
LOCUS       DAOTAX010000161         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_47087_length_1850_cov_23.961003, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000161 DAOTAX010000000
VERSION     DAOTAX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47087_length_1850_cov_23.961003"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1261)
                     /gene="buk"
                     /locus_tag="PLM04_08880"
     CDS             complement(143..1261)
                     /gene="buk"
                     /locus_tag="PLM04_08880"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HON03220.1"
                     /translation="MSENIINLHKNVDTNPTPMFKILSINPGSTSTKFAVYEDEKILF
                     LHTILHSTEELKSFEKIIDQLDFRKNLILNSLKHDGVDLQTLSAIVGRGGLIRPVASG
                     VYLVNEKMIADLREAKKGEHASNLGGIIAYEISRDIPECKPFIVDPVVVDEMQDVARI
                     SGLPQFPRRSMFHALNQKAVARKYAASVSAGYENLNLVIAHLGGGISVSAHAKGKVID
                     TNQGLGGYGPFSPERSGTIDAGLLVDFCFQGQASKEEIKKMLVGRGGLIAHLGTNDAY
                     LAEQKAVNGDTHAKLIMEAMAYNVAKEIGAMCTVLKGKVDAIILTGGISRSEWFVNYV
                     KEMVSSFGKLVVYPGENEVEALAMGALRVLRSESYKEY"
     gene            1505..>1850
                     /gene="rimP"
                     /locus_tag="PLM04_08885"
     CDS             1505..>1850
                     /gene="rimP"
                     /locus_tag="PLM04_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HON03221.1"
                     /translation="MIEQTTILQIVENFVSGTDFYIVEVKVSPENQILVVFDSFEGIS
                     IDDCEALNNFIESKLDREQEDYELEVSSPGLTEPFKVLKQYEKNKGNEVEIVTKKGEK
                     FSGILTEVNFDNC"
BASE COUNT          564 a          348 c          334 g          604 t
ORIGIN      
        1 tgaatatgcc tctgctgccg cacgattgca tcgtgtggca gcgtggggtt tggtgagaag
       61 aaaaatttgt aactgaaaaa gaaaagaatg aaaatttatc gatttccgaa tgattttttt
      121 aagtattgaa ttgattgatc gattaatatt ctttgtaact ttcactgcga agtacgcgca
      181 aagctcccat ggccaatgct tccacttcgt tttcgcccgg atatacgact aattttccga
      241 atgaggaaac catttctttc acgtaattta caaaccattc acttctactg attcctcccg
      301 ttagaattat ggcatcaact tttcctttta aaacggtgca catagctcca atttcctttg
      361 caacattata tgccatagct tccattatca gtttggcatg ggtatctcca ttcacagctt
      421 tttgttcggc caaataagca tcattggttc caagatgtgc tatcagtcca cctcttccga
      481 ccagcatttt ttttatttct tctttggaag cttgaccttg aaaacaaaag tccaccagca
      541 atccggcatc aatcgttcct gatctttctg gcgaaaacgg cccataaccg cccaagcctt
      601 gattggtgtc tatcactttt ccttttgcat gtgccgaaac actgattcct ccacccaaat
      661 gagcaattac caaattcaaa ttttcgtaac cggcgcttac tgaagcagca tattttcttg
      721 ctacagcttt ttggttgagc gcatgaaaca tggaacgacg aggaaattga ggcaatccac
      781 tgatgcgggc aacatcctgc atctcgtcca ccaccacggg atcaacgata aagggcttgc
      841 attctggaat atcgcgggat atttcatagg caatgatgcc gcccaaatta cttgcatgtt
      901 ctcctttttt ggcctctctc aaatcggcaa tcattttttc gttcaccaaa tatactcctg
      961 aagctaccgg tcgaatcagt cctcctctgc caacaatagc ggaaagtgtt tgtaaatcta
     1021 caccatcatg cttcagactg ttaagaatta aattttttct gaaatcgagc tgatcaatta
     1081 ttttttcaaa cgattttaat tcttccgttg aatgtaaaat ggtatgaaga aataaaattt
     1141 tttcgtcctc atagacggca aattttgtag aagtagaacc gggattgatt gaaaggattt
     1201 taaacatggg tgtaggattt gtgtcaacat ttttatgcaa atttatgata ttttctgaca
     1261 tattctaaat atttgatttc tttaaaggaa aaatctttgt tttaagtaat gtggttgcaa
     1321 taccttaaat aattcataat cagtcataaa gttataattg gttgttttat tgtaaaaaga
     1381 gaaaatgttt atttgataaa accttggatt ttatataatt attttgtaat tttgcaacgt
     1441 gaaaagaaat tgtgagaggg gacaaaattg tccccttgtt ttttagataa atgtgtactt
     1501 tccgatgatt gaacagacaa ccatattgca aatagtagaa aattttgttt caggcacaga
     1561 tttttacatt gtcgaagtta aagtgagccc tgaaaatcag attcttgtcg tttttgattc
     1621 atttgaaggt atttccattg acgattgcga agctttgaac aatttcatcg aatccaaact
     1681 ggacagagaa caggaagatt atgaattgga agtcagctct cccggactga cagaaccctt
     1741 taaagtactg aaacagtacg aaaaaaacaa aggtaatgaa gtggaaattg ttacaaaaaa
     1801 aggagaaaaa ttttccggaa tactcacaga ggtaaatttt gataattgta
//
LOCUS       DAOTAX010000162         1837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_47508_length_1837_cov_29.984287, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000162 DAOTAX010000000
VERSION     DAOTAX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47508_length_1837_cov_29.984287"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..462)
                     /gene="raiA"
                     /locus_tag="PLM04_08890"
     CDS             complement(163..462)
                     /gene="raiA"
                     /locus_tag="PLM04_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HON03222.1"
                     /translation="MKSRIQAINFDATEELVAYVEKKISKFEKLIDGILNVEVYLKLV
                     KPKISADKEAGIKVSIPNVDFFSSKACETFEEAIDLCVDAIEKQIKKHKEKISKK"
     gene            complement(477..1364)
                     /locus_tag="PLM04_08895"
     CDS             complement(477..1364)
                     /locus_tag="PLM04_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HON03223.1"
                     /translation="MVIMNEFLEYLRYEKNYSPYTVLSYQTDLSQFIEFMHETPETFT
                     PSQVTPQQIQQWIMLLMSEKKSARTLSRKISTLKSFWHFLLSRGYVAKNPVSNIILPK
                     KSKPLPAFFKKEEMAAALEETFLTDDFEKLRNHLMLELFYFSGIRRSELISLRDSDVD
                     FISRQLSVIGKRNKERIIPLNKKLCEDIRHYIQVRNKTIENKSQWLFVRKNGDKLYPK
                     LVYNMVHQTMSAYSSQSKRSPHVIRHSFATHLLNEGADINAVKELLGHSSLAATQVYT
                     HTSFEELYKIYKQSHPRGK"
     gene            complement(1431..1622)
                     /gene="rpsU"
                     /locus_tag="PLM04_08900"
     CDS             complement(1431..1622)
                     /gene="rpsU"
                     /locus_tag="PLM04_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HON03224.1"
                     /translation="MIVVPVKEGENIDKALKKFKRKFEKTGVVKELRRRQCFDKPSVI
                     KRNQKIRAVYLQKLQQEEK"
BASE COUNT          540 a          330 c          322 g          645 t
ORIGIN      
        1 caacgacccc gagattacaa atcacgtgct ctggccagct gagctaaaga ggcaattgtc
       61 taaaaaacac cattttcgtg tttaaaaagc cgcttaaata ttcctactta aacggcttgc
      121 aaatttactg aattattttt aatttcaaaa cctttttgag aattattttt tagaaatttt
      181 ttccttgtgc ttttttattt gtttttcgat ggcatcaaca caaagatcta ttgcttcctc
      241 aaaagtttcg caggccttag aggaaaagaa atctacattt ggaatagata cttttatccc
      301 tgcttccttg tcggccgaaa ttttcggctt aaccaacttc aaatagactt ccacgttcaa
      361 aattccatca atgagtttct caaattttga gattttcttt tctacatacg ctactaattc
      421 ttctgtcgcg tcaaagttaa tggcttgaat tcttgatttc ataatgacct ccttttttat
      481 tttccacgtg gatgagattg tttgtaaatt ttataaagtt cctcaaaact ggtatgggta
      541 tacacttgtg tagcagccaa actgctatgt cccaagagtt cttttactgc attgatgtct
      601 gcgccttcgt tcagcaagtg ggttgcaaag ctatgacgaa tgacatgcgg actccgtttg
      661 gactggctgc tatatgcaga catggtttgg tgtaccatgt tataaacaag tttaggataa
      721 agtttgtctc cattctttct aacaaaaagc cactgacttt tgttttcaat agttttgttt
      781 cttacctgaa tataatgccg aatatcttcg cacaattttt tatttaacgg gataatgcgt
      841 tctttattcc gttttccgat aacgcttagt tgccgactga taaaatcgac atcggaatcg
      901 cgaagtgaaa tgagctctga tcggcgaatt cccgaaaaat aaaaaagctc cagcatcaag
      961 tgatttcgaa gtttttcaaa atcgtcagtc aaaaaagttt cttccaaagc tgcagccatt
     1021 tcttcttttt tgaaaaaagc tggaagcggt tttgactttt ttggtaagat aatgtttgaa
     1081 accggatttt tcgccacata acctctcgaa agcaaaaaat gccagaaaga ttttagggtg
     1141 gagattttgc gcgaaagagt gcgcgccgat ttcttttcgg acattaacag cataatccat
     1201 tgctgaattt gctgaggagt aacctgagaa ggtgtaaaag tctcaggcgt ttcgtgcata
     1261 aattcaatga attgcgagag gtcggtctga tacgataaaa cggtataagg agaataattc
     1321 ttctcatacc gtaaatattc taaaaattcg ttcattatga ccataagttc aattatgcaa
     1381 cgaaaataga atgaaaaaac cggaaaagca aactttttaa cggatttttt ctatttttct
     1441 tcctgctgta atttttgcag ataaactgca cggattttct gattccgttt gataacagaa
     1501 ggtttatcga aacactgacg acggcgaagt tccttaacaa cacctgtttt ctcaaatttg
     1561 cgtttgaatt ttttcaaagc tttgtcaatg ttttctcctt ctttaactgg aactacgatc
     1621 ataattttta attataaatt tggttttgaa tagtaatttt atggtttcta aaaatggtgt
     1681 gcaaagataa tcatttgttc tgaaaaatca aatatttttt taatgttgat atttttggaa
     1741 gtttattttt ccttctgcgc gggagaaaat tacatattta atttttcaac cgctgaatga
     1801 gctcttccaa cgaaatcatt tgttgctcac cggtagc
//
LOCUS       DAOTAX010000163         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_50004_length_1767_cov_27.528621, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000163 DAOTAX010000000
VERSION     DAOTAX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50004_length_1767_cov_27.528621"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1767
                     /locus_tag="PLM04_08905"
     CDS             <1..>1767
                     /locus_tag="PLM04_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HON03225.1"
                     /translation="NQKGIYVVELTSAPVEKAKSVFYTAEIKKFLHNRHWESGHGKAY
                     IYLQKDSLSGNLVFGDRLMLETEFKSPEKLLNPDGFDYATYLKRQGVGATAYIASQHW
                     QKIGHNQAFSIRRMADQCRNYLLGIYRKFNIQGNEFGVLAALTLGYTEDLQPDLRKSF
                     SASGATHILSVSGLHVGTVYAVLLFIFGFLNHNQRQKISKIIIITLCMWGYAFITGLS
                     PSVVRATLMFTFVALANCLERKSQIYNTIFMSAFFMLLVNPNFLFDIGFQLSYSAVLS
                     IIFFQPPLTKLLPTNHRFLKWGRDLLLVSVAAQIGTAPFTLYYFQQFPNYFLLTNLLV
                     LPLSTLVIYLAMGLLAVSFIPYLSTAVAFLLKISVGLLNFCIVQIEHLPYSLSYISLD
                     IKQTILIFMVIFSLSAYYYNKKFVPLALGLISLLLVCVVDLQIKYNTLRNQKIIVFAG
                     QKSTHVNFIDHGKNYVYTTDSVELEKIASNYWRKNKLQNPEYLSNSNWFENGFASFYG
                     IKILILTDEQLKRKVTETPLNIDLLIIGNHLKPRMDQILSCVQPQKIIVDKSISKWYT
                     NDIRQQCEKHGIEFYSVADQGA"
BASE COUNT          517 a          339 c          332 g          579 t
ORIGIN      
        1 tgaaccaaaa agggatttat gtggttgaat tgacttcggc tccggtagaa aaagcaaaat
       61 cggtttttta tacggccgag ataaaaaaat ttcttcataa ccgacattgg gaatcgggac
      121 atggcaaagc ttacatttat cttcaaaaag acagtttgtc aggcaatttg gtttttggcg
      181 accgtttgat gcttgagact gaatttaaaa gccctgaaaa gttgctgaat cctgatggtt
      241 ttgattatgc tacttatctt aaaagacagg gagtgggagc aactgcctat attgcttcac
      301 aacactggca aaaaattggt cataatcaag ctttttcgat tcgtcgcatg gcagatcaat
      361 gcaggaatta cctgctggga atttacagga aatttaatat tcaaggaaac gaatttgggg
      421 ttttggcagc tttgacgctg ggttatactg aagatttaca acccgattta cggaaaagtt
      481 tcagcgcttc gggagcaaca catatacttt cggtaagtgg acttcatgtg ggaacagttt
      541 atgccgtttt gctttttatt ttcggatttc tgaatcataa tcagcggcaa aaaatttcca
      601 agataattat tatcacttta tgtatgtggg gctatgcctt tattacgggt ttgtctcctt
      661 ctgtagtgcg ggcaacactc atgttcacat tcgttgcttt agcaaactgt ctggaaagaa
      721 aatcgcagat ttacaacacg attttcatgt cagccttttt catgctgctg gtgaatccga
      781 attttctttt cgatattggt tttcagctca gctactcggc cgttctgagc attattttct
      841 ttcaaccgcc cctgactaaa ttgttaccta ccaatcatcg atttttaaaa tggggaagag
      901 atttgcttct cgtttcagta gcagctcaaa ttggaaccgc tcctttcaca ttgtattatt
      961 ttcaacagtt ccccaattat tttctgttga ccaatctatt ggttttaccg ctctccacac
     1021 tcgtaatata tttggcaatg ggtttgctgg cagtttcatt tatcccctat ttgtccacag
     1081 ctgtggcttt tttgctgaaa ataagtgttg ggcttctcaa tttttgtatt gttcaaattg
     1141 aacatctccc ctactctctt tcttatattt ctctggatat aaagcaaacc attctaattt
     1201 tcatggtaat tttcagtcta tcggcatact attacaacaa gaaatttgta cccctcgcgt
     1261 tgggactcat ttcgctgctg ctggtttgtg tagtggattt acaaataaaa tacaatactc
     1321 tacggaatca gaaaataatt gtttttgccg gacagaaatc aacacatgtc aactttatcg
     1381 accacggaaa aaattatgtc tatactacag attcggtaga attggaaaaa attgcatcca
     1441 actactggcg gaaaaacaaa ttacaaaatc ctgaatacct ttcaaacagc aactggtttg
     1501 aaaatggttt tgcttctttc tacggcataa agattctgat tttaacagac gaacaactga
     1561 aaagaaaagt tacagaaact ccattaaaca ttgatttact gattattggc aatcatctga
     1621 aaccccgaat ggatcaaatt ctttcttgtg tgcaaccaca aaaaataatt gtcgacaaaa
     1681 gcatctcgaa gtggtatacc aacgacatcc gacaacaatg cgagaaacat gggattgagt
     1741 tttattctgt agctgatcaa ggtgcct
//
LOCUS       DAOTAX010000164         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_50418_length_1756_cov_33.323927, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000164 DAOTAX010000000
VERSION     DAOTAX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50418_length_1756_cov_33.323927"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1756
                     /locus_tag="PLM04_08910"
     CDS             159..>1756
                     /locus_tag="PLM04_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4980 domain-containing protein"
                     /protein_id="HON03226.1"
                     /translation="MKKFLIPLFAVLVILLAVGFKASKDKQQKKIKITTAYLLLPVEE
                     NADEVKTTIKYPDKKLPDIFWIRFAQRKVDYWVKLEVGKYKGKSLELIFDGPPASKFG
                     IKRIRPADQFKINYAEPYRPDFHFSPEYGWMNDPNGLTYFEGEYHLFYQYNPFGTRWG
                     NMSWGHAVSTDLTSWTYLPVALWPDTLGAIFSGSVVIDKNNTAGFGKNAMIAIYTSAG
                     KVQSQSLAYSTDKGRTFNKFEKNPIIPNPGITDFRDPKVSWYQPENKWIMALATKQTV
                     TFYSSLDLKNWNRESEFGEGIGSHAGVWECPDLFPLNYDGKTKWVLLVSINPGGPNGG
                     SATQYFVGDFDGKKFQADPLPYPLWIDYGRDNYAGVTFSNIPEIDGRRIFLGWMNNWD
                     YANQTPTKIFRSSNTVPRELQLVNNGNHCVLSSYPVKEVDLLRGETVDFPDQIVNNEK
                     SLKEILKQNDGSFELNMVLKPKKGNVFGFSLQNSKGETLDFIFDLSTQFFKIQRNKSG
                     LIDFSDRFPLGMNAPLASKRSYEIR"
BASE COUNT          562 a          279 c          359 g          556 t
ORIGIN      
        1 aaattttttg ttcagaagta attgattttt actgtttctc ccgttgtaaa ttcatttaat
       61 gggagaaaca gttatttgtt gcaattcatt gaaaattaat tccaaaaaaa gtattcttac
      121 taattgttag aaatttaagg taaccccaga aaacttttat gaaaaaattt cttattcctc
      181 tatttgctgt tttggttatt cttttggcgg taggattcaa ggcttcgaaa gataagcagc
      241 aaaagaaaat taaaattact actgcctatt tgttgttacc cgttgaagaa aacgccgacg
      301 aagttaaaac cacaataaaa tatcctgata aaaaattgcc ggatattttt tggattcgtt
      361 ttgctcagcg taaggtggat tactgggtaa aattagaagt tgggaaatat aagggcaaaa
      421 gcttggaatt aatttttgat ggtcctcctg catccaagtt cggaataaag aggataaggc
      481 cagccgatca attcaagatt aattacgctg aaccttatcg tcctgatttc catttttctc
      541 ctgaatatgg ttggatgaac gatcccaacg gtttgactta ttttgaaggc gaataccatc
      601 ttttttatca gtataatccc tttggaacaa gatggggaaa tatgagctgg ggtcatgccg
      661 taagcaccga tctaacctca tggacttact tgcctgtggc tttatggccc gatacattgg
      721 gggcaatttt ttcgggaagc gtcgttattg ataaaaataa tactgccggt tttgggaaaa
      781 atgccatgat tgctatttat accagtgccg gaaaagttca atcgcaatcg cttgcttaca
      841 gtacagataa aggaagaacc tttaataagt ttgagaaaaa tcccattatt ccaaatcccg
      901 gtataactga ttttcgcgat ccgaaagtga gttggtatca gcctgaaaat aaatggataa
      961 tggcattagc aacaaaacaa acggtaactt tttattcttc acttgacctt aaaaactgga
     1021 atcgggaaag tgaattcggt gaaggaattg gctcacatgc aggagtgtgg gaatgtcctg
     1081 atttatttcc attaaactat gatggtaaaa caaagtgggt cttattggta agcattaatc
     1141 ccggaggacc taatggggga tctgctactc aatatttcgt tggagatttt gatggcaaaa
     1201 aatttcaggc tgatcctttg ccttatcctc tttggataga ttatgggcga gacaactatg
     1261 caggcgtcac ttttagcaat attcccgaaa tagatggtcg tcgaattttc ctcggttgga
     1321 tgaacaactg ggattatgcg aatcaaacac caacaaaaat ttttagaagt agtaacactg
     1381 ttccccgaga attgcagctt gtaaataatg gtaatcattg cgtgttaagt agttatccag
     1441 taaaagaggt cgatttgctc agaggcgaaa cagttgattt cccagatcaa attgtaaata
     1501 atgagaaatc gctgaaagaa atattgaagc agaatgatgg cagttttgaa cttaatatgg
     1561 ttttaaaacc caaaaaagga aatgtttttg gattttcgct acagaattca aaaggtgaaa
     1621 cattggattt tatttttgat ttgtccactc aattttttaa aattcaaagg aataaaagcg
     1681 ggcttattga tttcagcgat cgttttccat tgggaatgaa tgctccatta gcgtctaaaa
     1741 gaagttatga aattcg
//
LOCUS       DAOTAX010000165         1676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_53568_length_1676_cov_34.417027, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000165 DAOTAX010000000
VERSION     DAOTAX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53568_length_1676_cov_34.417027"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1207)
                     /locus_tag="PLM04_08915"
     CDS             complement(398..1207)
                     /locus_tag="PLM04_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03227.1"
                     /translation="MKRTTLLVSLFLATVAFTNAQTNLLENPGFETWGETLPDPWYIV
                     SKSVTGVTPAASTTIFAEGTKSLKIDATGASSTFNMAQGVTIVPEHSYSFSAKCYVES
                     GDGTDARLWCNFKKDATTYFTEDELIATGLYATLRSGNENSSGSSYLATTIGSWFTAT
                     GTFTAPADAVGFDFQFRTYKKAVVYWDDMQLIDNSTSGIISPKAESLKVWTDAGKIKF
                     HAVSGEKVEIFNTVGQSLHRALATEGINSVSIAERGIMIVKVGNRIGKVVL"
     gene            complement(1356..>1676)
                     /locus_tag="PLM04_08920"
     CDS             complement(1356..>1676)
                     /locus_tag="PLM04_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03228.1"
                     /translation="GLTPATTYYYTVTAKNGSYVSSASDEASASTLGTATSNVAVRGI
                     FVRNGQVVVTAPAAGERIEIFNAVGQRLVSKLSTEGENTISVSAKGVLVVKFGSEVTK
                     VILP"
BASE COUNT          511 a          354 c          312 g          499 t
ORIGIN      
        1 tttttaaagg tctgatggaa cccacatgtc tgaatattat attcattcgt gtttgagtat
       61 gttatacatg tgggtttcat aaaattttca tcaaaacatt ttgcacaaat atacaggata
      121 attctgaatt gaaccagcag agaaagataa attgaactta gaacaaaagt aaaatcttta
      181 cctgcagcaa atcaataaat ttttcctttt ggattaaaaa aataccataa aaaatttttt
      241 aaaccggttt tcagcatttc catactttta taaataattc aatatcatca ttaatcgtat
      301 aatttttggt gcttaaatgc aaaaaaaacg aggcactgtc ccgacgacaa cgccccgctt
      361 ttaaaaaact gtcaaacaaa atgcaacagc tgcgaattta caaaacaacc ttgccgattc
      421 gattgcccac ttttactatc atgatacctc gttcagcaat gcttacggaa ttgataccct
      481 cggttgccaa agctctgtgg agagattgtc ccacagtatt gaaaatttcc actttttcgc
      541 ccgaaacagc atggaactta attttccctg cgtctgtcca tactttcagc gattcggctt
      601 tcggtgaaat gataccggaa gtggaattgt cgatcaactg catgtcgtcc caataaacaa
      661 cggctttttt gtaggtacgg aactgaaagt caaaacctac agcatcagcc ggcgctgtaa
      721 atgttccggt agcggtgaac cacgaaccta ttgtggttgc taaataggat gaaccggagg
      781 agttttcgtt tccacttctc aaggttgcat ataaacccgt agctattaat tcatcttcag
      841 taaaatacgt tgttgcatcc tttttgaaat tgcaccacaa tcgggcatct gtgccatcac
      901 ctgattctac ataacatttg gccgaaaagc tatagctatg ctcgggtaca atggtaacac
      961 cctgcgccat gttgaacgta ctgcttgctc cggtagcatc aattttaagc gatttggttc
     1021 cttctgcaaa tatggttgta ctggcagcag gtgtaacgcc cgtaacactt tttgaaacga
     1081 tataccacgg atcgggcaat gtttctcccc atgtttcaaa tcccggattt tcaagcaagt
     1141 ttgtttgtgc atttgtaaag gctacggttg ccaaaaataa cgaaaccaat aaagtagttc
     1201 ttttcatgtc tgtactgttt ttaaaatgaa taaataatta tgtttataat agaatgtcca
     1261 aaagtaacaa aaatttttat taaaagaaaa attcggaagt gataaaccta acaggtttcg
     1321 gaaacctgtt aggttttatc tgttttatcg gttttttacg gcagtatcac tttggtaact
     1381 tccgaaccga attttaccac caatacgccc ttagccgaaa cggaaatggt gttttcgcct
     1441 tccgtagaaa gtttagaaac caatcgctga cccacagcat tgaaaatctc tattctttcg
     1501 ccggcagcag gggctgttac cactacctga ccattgcgta caaaaatacc ccgaacggca
     1561 acattggaag tggcggtgcc cagcgtggaa gcagaagcct catccgatgc ggacgaaaca
     1621 taactgccgt tctttgccgt taccgtataa taatacgtgg tggcaggcgt caaacc
//
LOCUS       DAOTAX010000166         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2022
            NODE_53610_length_1675_cov_36.967284, whole genome shotgun
            sequence.
ACCESSION   DAOTAX010000166 DAOTAX010000000
VERSION     DAOTAX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864089
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,749
            Genes (coding)                    :: 1,731
            CDSs (with protein)               :: 1,731
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53610_length_1675_cov_36.967284"
                     /isolate="AMR_MDS_2022"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLM04_08925"
     CDS             <1..318
                     /locus_tag="PLM04_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HON03229.1"
                     /translation="EENLNDLCASLQATVIDILMDKLKKAAKDYNIHQIALAGGVSAN
                     SALRQAFNDLATIDGYEVFIPPFAFTTDNAAMIAMTGYFKYLDHDFSSFDDVPFARVT
                     NTF"
     gene            326..919
                     /locus_tag="PLM04_08930"
     CDS             326..919
                     /locus_tag="PLM04_08930"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HON03230.1"
                     /translation="MKKELSRIKKNLTPYNGVIYFVFILLICHFAWKFLVRGDDSNRQ
                     VTFLGNDISAPFQFMANHVAQTTASVLNFLGFHIQLFANNVLKQENGFGIQIVWSCTG
                     LKQAYIFFCIIAFYCGPMRKKLWFIPLGLAVVYVFNIFRIAAITALVQQHSSWFTFLH
                     EYFFKYLFYAVIFALWVLWEEKFVTKKEKNETHMTQT"
     gene            930..1106
                     /locus_tag="PLM04_08935"
     CDS             930..1106
                     /locus_tag="PLM04_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HON03231.1"
                     /translation="MNAVKATDISSQVEESVKTLVRKIQGTRSTAKKTETQKADMTAE
                     GKEVKEISTLQDVL"
BASE COUNT          517 a          277 c          319 g          562 t
ORIGIN      
        1 gaggaaaatc tcaatgattt gtgtgcttct ttgcaggcaa ccgttatcga tattttgatg
       61 gataaactga aaaaggccgc caaggattat aacattcacc aaattgcact ggctggaggt
      121 gtttcggcta attcagcctt gcgtcaggct tttaacgatt tggcaactat tgatggttac
      181 gaagttttta ttcctccttt tgcttttact accgacaatg ctgccatgat tgccatgacc
      241 ggttatttca agtatctcga tcacgatttc agttcctttg acgatgtacc gtttgcaaga
      301 gttaccaata ctttttaaga atatcatgaa aaaggagctt tccagaataa aaaaaaattt
      361 gactccttac aacggtgtaa tttattttgt ttttattttg ctgatttgtc attttgcatg
      421 gaaatttctg gtgcggggtg atgattcaaa tcgtcaggtt acgtttttgg gaaatgatat
      481 ttcggcgcca tttcaattta tggccaacca tgttgctcaa actactgcaa gcgttttaaa
      541 ttttttagga tttcatattc agctttttgc caataacgtt ctgaaacagg aaaatggctt
      601 cggtatccaa attgtatggt cgtgtaccgg cctgaaacag gcttatattt ttttctgtat
      661 catagctttt tattgtgggc cgatgagaaa aaaattgtgg ttcatcccgc tcggactggc
      721 tgtggtttat gtgtttaata ttttcagaat cgccgcaata acggctcttg ttcagcaaca
      781 ctcttcttgg tttacttttt tgcacgaata ttttttcaaa tacttatttt atgctgtaat
      841 ttttgcttta tgggttttgt gggaagaaaa atttgtaact aaaaaagaaa agaatgaaac
      901 ccacatgaca caaacttgac acccgagtaa tgaacgccgt taaagccaca gatatctctt
      961 cccaagtaga agaaagcgta aaaacattgg ttcgaaaaat tcagggaaca agatccactg
     1021 caaagaaaac agaaacgcaa aaagcggaca tgacagccga aggaaaagaa gtcaaagaaa
     1081 tatccaccct acaggatgtt ctttaaatga ctaaagttgg cgttcgtcgc aggttgtctc
     1141 ataatgacca aaattagaaa attgactttc aatctgttag taattgtatg aaaaatcccc
     1201 aaaacagaat aaattcaaca aaattatttt gctgtattgg gagttttctt tgtcagtagg
     1261 ttttaaattg taagtttttg cattttgaga attccttttg agacaccctc ttcttgtatt
     1321 ataaggattt ataatccgct aagttaaata tttagattaa aaagttatct ttgctcaata
     1381 aatatatttt gccgtggaaa gtgtttcgga ttacaaacct ttataataca ttacgaggtg
     1441 attgcaatta ttctcttctg tcatgaccgt tggttcacct cagacgcccg caaagctttg
     1501 tatattgaat gaggatatcc ggtaggacgg ggattgtgaa aaaaacttca taaattttgt
     1561 ttattaaatg aaaagttttt gtaattttga atcgaaagat aaaacgcaac ataatagcgt
     1621 ctatccatcc aaatatggaa ataaggtgct atgaagtagc gtgtataaat gagtt
//
